# Patent

## Title
Immunostimulatory oligonucleotides and uses thereof

## Abstract

Oligonucleotides containing the non-palindromic sequence motif:

X1X2X3X4X5X6X7X8,

wherein X1 is C,T,G or A (preferably T or C); wherein X2 is C,T,G or A; wherein X7 is C,T,G or A (preferably G); at least three, and preferably all, of X3, X4, X5, X6 and X8 are T; and with the proviso that, in the motif, a C does not precede a G (in other terms, the nucleic acid motif does not consist of a CpG oligonucleotide), that modulate the immune response of animals of the order Primate, including humans, are disclosed. This immune modulation is characterized by stimulation of proliferation, differentiation, cytokine production and antibody production on B-cells and cell differentiation on plasmacytoid dendritic cells.

## Background
This application is a continuation of U.S. patent application Ser. No. 10/309,775 filed on Dec. 4, 2002, now U.S. Pat. No. 7,038,029, which claims priority to Canadian Patent Application No. CA 2,388,049, filed May 30, 2002.


FIELD OF THE INVENTION
The present invention relates generally to oligonucleotides containing the non-palindromic sequence motif:

X1X2X3X4X5X6X7X8,

wherein X1 is C,T,G or A (preferably T or C); X2 is C,T,G or A; X7 is C,T,G or A (preferably G); at least three, and preferably all, of X3, X4, X5, X6 and X8 are T; with the proviso that, in the motif, a C does not precede a G, that are immunostimulatory in animals of the order Primate, including humans.

RELEVANT REFERENCES
Patent Documents


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Krug A, Rothenfusser S, Hormung V, Jahrsdorfer B, Blackwell S, Ballas Z K, Endres S, Krieg A M, Hartmann G. Identification of CpG oligonucleotide sequences with high induction of IFN-α/β in plasmacytoid dendritic cells. Eur. J. Immunol. 31, 2154-2163, (2001).
Krug A, Towarowski A, Britsch S, Rothenfusser S, Hornung V, Bals R, Giese T, Engelmann H, Endres S, Krieg A M, Hartmann G. Toll-like receptor expression reveals CpG DNA as a unique microbial stimulus for plasmacytoid dendritic cells which synergizes with CD40 ligand to induce high amounts of IL-12. Eur J Immunol. 31, 3026-3037, 2001.
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Krug A, Towarowski A, Britsch S, Rothenfusser S, Hornung V, Bals R, Giese T, Engelmann H, Endres S, Krieg A M, Hartmann G. Toll-like receptor expression reveals CpG DNA as a unique microbial stimulus for
plasmacytoid dendritic cells which synergizes with CD40 ligand to induce high amounts of IL-12. Eur J Immunol. 31:3026-37 (2002).
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Krug A, Rothenfusser S, Hornung V, Jahrsdorfer B, Blackwell S, Ballas Z K, Endres S, Krieg A M, Hartmann G. Identification of CpG oligonucleotide sequences with high induction of IFN-alpha/beta in plasmacytoid dendritic cells. Eur J Immunol. 31:2154-63 (2001).
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Decker T, Schneller F, Sparwasser T, Tretter T, Lipford G B, Wagner H, Peschel C. Immunostimulatory CpG-oligonucleotides cause proliferation, cytokine production, and an immunogenic phenotype in chronic lymphocytic leukemia B cells. Blood. 95:999-1006 (2000).
Decker T, Schneller F, Kronschnabl M, Dechow T, Lipford G B, Wagner H, Peschel C. Immunostimulatory CpG-oligonucleotides induce functional high affinity IL-2 receptors on B-CLL cells: costimulation with IL-2 results in a highly immunogenic phenotype. Exp Hematol. 28:558-68 (2000).
Jahrsdorfer B, Hartmann G, Racila E, Jackson W, Muhlenhoff L, Meinhardt G, Endres S, Link B K, Krieg A M, Weiner G J. CpG DNA increases primary malignant B cell expression of costimulatory molecules and target antigens. J Leukoc Biol. 69:81-8 (2001).
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BACKGROUND OF THE INVENTION
The Immune System
The major function of the immune system is to protect the host of invading pathogens. A number of different cell types, both antigen-independent and antigen-specific, have evolved to detect and neutralize these invading pathogens. Among them, lymphocytes have an important characteristic, which is their ability to specifically recognize antigens, a feature not possessed by any other cell. This means that any lymphocyte function stimulated by an antigen is directed solely at that antigen.
Lymphocytes may be divided into two major populations: T and B. T lymphocytes have a central role in regulating the immune response and for this they produce and secrete lymphokines (i.e.: interleukins). B-lymphocytes are the only cells that produce antibodies, which are proteins—Immunoglobulins (IgG)—that recognize and bind antigens.
Some T lymphocytes are known as helper (Th lymphocytes) because they assist B cells to produce antibody. T-lymphocytes express a characteristic membrane molecule designated CD4. Other T lymphocytes are known as cytotoxic (CTL) because they are capable of killing certain cells. They express a different characteristic membrane protein designated CD8.
Th lymphocytes, in mice, have been subdivided according to the lymphokines they produce in groups designated Th0, Th1 and Th2. In general, Th 1 lymphocytes produce lymphokines which stimulate macrophages and CTLS (IL2, IFNγ, TNF-β), Th 2 lymphocytes produce lymphokines which stimulate B lymphocytes to proliferate and produce antibody (IL 2, IL5, IL6, IL10, IL13), whilst Th 0 lymphocytes produce a mixture of lymphokines and are thought to be an intermediate stage from which Th 1 and Th 2 lymphocytes are derived. In humans, Th1 and Th2 like lymphocytes have been demonstrated, although they do seem to show a less strict division with respect to their patterns of cytokine secretion.
A third population of lymphocytes, which lack the major makers of T and B cells include the natural killer cells (NK cells), the killer cells (K cells) and the lymphokine-activated killer cells (L A K cells). NK cells can kill certain tumor cells and some virally infected cells, but unlike cytotoxic T lymphocytes they are not capable of recognizing a specific antigen. K cells are able to bind to cells, which have antibody to them via their antigen-binding regions and kill them. L A K cells do not specifically recognize an antigen but they are capable of destroying a wider range of targets a NK cells.
Macrophages and dendritic cells play a critical role in initiating immune responses, helping T cells to respond to antigens.
There are several antibody classes. The IgG class comprises most of the circulating antibodies and it has four subclasses designated IgG1, IgG2, IgG3 and IgG4.
The IgM class comprises about 10% of the circulating antibodies. These are the principal antibodies produce during the primary immunological response. The IgA class comprises most of the antibody secreted at mucous membranes and exerts its protective effect by blocking access of the antigen to the inner body. The IgD class comprises less than 1% of serum antibodies and its biological role is largely unknown. The IgE class comprises antibodies that are mainly bound to the surface of most cells and basophils. These antibodies are associated with reactions that occur in individuals who are undergoing allergic reactions.
Vaccines and Vaccines Adjuvants
Vaccines are preparations used to stimulate animals to mount an immune response against antigens included in the vaccine.
Vaccines often include adjuvants, which are substances that used in combination with specific antigen produce more immunity than the antigen used alone. (Ramon, G., 1926. Procedes pour accroite Ia production des antitoxins. Ann. Inst. Pasteur. 40, 1-10).
Many kind of compounds function as vaccine adjuvants (Edelman, R., 2000. An overview of adjuvant use, in: Vaccine Adjuvants. Preparation Methods and Research Protocols. D. T. O'Hagan, Ed., Humana Press, Totowa, N.J. References cited in this article are incorporated herein as background material).
However, currently, the only adjuvants approved for use in humans are aluminum salts (Gupta, R. K. and Rost, B. E., 2000. Aluminum compounds as vaccine adjuvants in: Vaccine Adjuvants. Preparation Methods and Research Protocols. D. T. O'Hagan, Ed., Humana Press, Totowa, N.J.) and the oil-in-water emulsion M F 59 (Ott, G. Radhakrishman, R. Fang, J. and Hora, M., 2000. The adjuvant M F 59: A 10-Year Perspective, in : Vaccine Adjuvants. Preparation Methods and Research Protocols. D. T. O'Hagan, Ed., Humana Press, Totowa, N.J.).
Nucleic Acids as Immunostimulatory Compounds
Several polynucleotides have been demonstrated to have immunostimulatory properties. For example, poly (I,C) is an inducer of interferon (IFN) production, macrophage activation and NK cell activation (Talmadge, J. E., Adams, J., Phillips, H., Collins, M., Lenz, B., Schneider, M., Schlick, E., Ruffmann, R., Wiltrout, R. H., Chirigos, M. A. 1985. Immunomodulatory effects in mice of polyinosinic-polycytidylic acid complexed with poly-L:-lysine and carboxymethylcellulose. Cancer Res. 45:1058; Wiltrout, R. H., Salup, R. R., Twilley, T. A., Talmage, J. E. 1985. mmunomodulatory of natural killer activity by polyribonucleotides. J. Biol. Resp. Mod. 4:512), poly (dG,dC) is mitogenic for B cells (Messina, J. P., Gilkerson, G. S., Pisetsky, D. S. 1993. The influence of DNA structure on the in vitro stimulation of murine lymphocytes by natural and synthetic polynucleotide antigens. Cell. Immunol. 147:148) and induces IFN and NK activity (Tocunaga, T., Yamamoto, S., Namba, K. 1988. A synthetic single-stranded DNA, poly(dG,dC), induces interferon-α/β and -γ, augments natural killer activity, and suppresses tumor growth. Jpn. J. Cancer Res. 79:682).
Bacterial DNA has also been reported to have immunostimulatory properties. These properties include the induction of cytokines (interferon gamma (IFN γ), alpha (IFN α), beta (IFN β); tumor necrosis factor alpha (TNF α), interleukin 6 (IL6), 12 (IL 12) and 18 (IL 18), as well as the direct stimulation of B cells (Yamamoto, S. et al. 1988. In vitro augmentation of natural killer cell activity of interferon α/β and γ with deoxyribonucleic acid fraction from Mycobacterium bovis BCG. Jpn. J. Cancer Res. (Gann) 79: 866-873; Yamamoto S. et al, 1992. DNA from bacteria, but not from vertebrates, induces interferons, activates natural killer cells and inhibits tumor growth. Microbiol. Immunol. 36: 983-997.; Klinman, D. M., Yi, A-K., Beaucage, S. L., Conover, J. and Krieg, A. M., 1996.
CpG motifs present in bacterial DNA rapidly induce lymphocytes to secrete interleukin 6, interleukin 12 and interferon γ. Proc. Natl. Acad. Sci. USA 93, 2879-2883. Halpern, M. D., et al. 1996. Bacterial DNA induces murine interferon -γ production by stimulation of interleukin-12 and tumor necrosis factor-α. Cell. Immunol. 167: 72-78. Sparwasser, T. et al, 1997. Macrophages sense pathogens via DNA motifs: induction of tumor necrosis factor-α-mediated shock. Eur. J. Immunol. 27: 1671-1679; Krieg, A. M. et al., 1995. CpG motifs in bacterial DNA trigger direct B-cell activation. Nature 374: 345-349).
In contrast, it has been reported that mammalian DNA has no significant immune effects (Pisetsky, D. S. 1996. The immunologic properties of DNA. J. Immunol. 156: 421-423 ; Messina et al. 1991. Stimulation of in vitro murine lymphocyte proliferation by bacterial DNA. J. Immunol. 147: 1759).
Synthetic DNA has also been reported to be immunostimulatory if contains unmethylated CpG motifs. (Yamamoto, S et al.; 1992. Unique palindromic sequences in synthetic oligonucleotides are required to induce INF and augment INF-mediated natural killer activity. J. Immunol. 148: 4072-4076; Ballas, Z. K., et al.; 1996. Induction of NK activity in murine and human cells by CpG motifs in oligodeoxynucleotides and bacterial DNA. J. Immunol. 157: 1840-1845; Hartmann, G., Krieg, A. M. 2000. Mechanism and function of a newly identified CpG DNA motif in human primary B cells. J. Immunol. 164:944; Hartmann, G., Weeratna, R. D., Ballas, Z. K., Payette, P., Blackwell, S., Suparto, I., Rasmussen, W. L., Waldschmidt, M., Sajuthi, D., Purcell, R. H., Davis, H. L., Krieg, A. M. 2000. Delineation of a CpG phosphorothioate oligodeoxynucleotide for activating primate immune responses in vitro and in vivo. J. Immunol. 164:1617; Verthelyi, D., Ishii, K. J., Gursel, M., Takeshita, F., Klinman, D. M. 2001. Human peripheric blood cells differentially recognize and respond to two distinct CpG motifs. J. Immunol. 166:2372). However, one oligonucleotide containing phosphorothioate bonds that lack CpG motifs has been found to have some immunostimulatory activity on human B cells (Liang, H., Nishioka, Y., Reich, C. F., Pisetsky, D. S., Lipsky, P. E. 1996. Activation of human B cells by phosphorothioate oligonucleotides. J. Clin. Invest. 98:1119). This particular non-CpG oligonucleotide containing phosphorothioate bonds is a poy-T chain, 20 nucleotides long. Also, Vollmer et al (Vollmer J, Janosch A, Laucht M, Ballas Z K, Schetter C, Krieg A M. Highly immunostimulatory CpG-free oligodeoxynucleotides for activation of human leukocytes. Antisense Nucleic Acid Drug Dev. 12:165-175, 2002) reported immunostimulation by phosphorothioate poly T ODNS. These authors pointed out that poly T ODNs are only active as phosphorothioate ODNs and have much lower activity than CpG ODNs.
It has now been discovered that non-CpG oligonucleotides containing the following non-palindromic sequence motif:

X1X2X3X4X5X6X7X8,

wherein X1 is C,T,G or A (preferably T or C); X2 is C,T,G or A; X7 is C,T,G or A (preferably G); at least three, and preferably all, of X3, X4, X5, X6 and X8 are T; with the proviso that, in the motif, a C does not precede a G, have potent immunostimulatory activity. Therefore, these oligonucleotides can be administered to subjects to treat “immune system deficiencies” or in conjunction with a vaccine, as adjuvants, to boost the immune system in order to have a better response to the vaccine or administered to subjects to increase the responsiveness to tumors.

SUMMARY OF THE INVENTION
It has now been discovered that non-CpG oligonucleotides containing the following non-palindromic sequence motif:

X1X2X3X4X5X6X7X8,

wherein X1, X2 and X7 are C,T,G or A; at least three of X3, X4, X5, X6 and X8 are T; with the proviso that, in the motif, a C does not precede a G, are useful as immunostimulants in animals of the order Primate, including humans.

According to a preferred embodiment, X1 consist of a C or a T and X7 consist of a G. More preferably X3,X4,X5,X6X7 X8 of the immunostimulatory motif consist of TTTTGT. Even more advantageously X1X2X3X4X5X6X7X8 consist of CNTTTTGT or TNTTTTGT wherein N is C, T, G or A. Those of X3-X6 and X8 that are not T can be any nucleotide (e.g., C, T, G, A) or can be absent so that the nucleotide preceding links directly with the nucleotide following the position of the omitted nucleotide (S). The oligonucleotides of this invention are useful as adjuvants in a vaccine formulation comprising one or more antigens. In embodiments of this aspect, the vaccine formulation can be liquid or lyophilized in dosage form. Many dosage forms are known in the art and can be applied herein. In embodiments of this aspect the oligonucleotides of this invention are present in the composition at a dose of from about 10 to 10,000 μg per dose. In these preparations the oligonucleotides of this invention may be combined with other immunostimulant compounds. Examples of well known immunostimulants are: α-interferon, β-interferon, γ-interferon, granulocyte macrophage colony stimulator factor (GM-CSF), interleukin 2 (IL2), interleukin 12 (IL12) and CpG oligonucleotide.
In certain embodiments, the invention relates to an immunostimulatory oligonucleotide having 24 to 100 nucleotides, comprising the nucleotide sequence TCTTCTTTTTGTCTTTTTGTCTTT (SEQ ID No 4). In certain embodiments, the immunostimulatory oligonucleotide is encapsulated in a slow release delivery vehicle.
In yet additional embodiments, the immunostimulatory oligonucleotide is included in a plasmid. In certain embodiments, the composition further comprises an antigen. In certain embodiments, the antigen is selected from the group consisting of viruses, bacteria, fungi, parasites, tumor cells, toxins, allergens, proteins, glycolipids, and polysaccharides. In additional embodiments, the antigen is a viral antigen, a bacterial antigen, a human or animal tumor cell antigen, or a fungal antigen.
In preferred embodiments the antigenic component of the vaccine is one or more, natural or recombinant, antigens of viruses like: Human immunodeficiency viruses, such as HIV-1 and HIV-2, polio viruses, hepatitis A virus, human coxsackie viruses, rhinoviruses, echoviruses, equine encephalitis viruses, rubella viruses, dengue viruses, encephalitis viruses, yellow fever viruses, coronaviruses), vesicular stomatitis viruses, rabies viruses, ebola viruses, parainfluenza viruses, mumps virus, measles virus, respiratory syncytial virus, influenza viruses, Hantaan viruses, bunga viruses, hemorrhagic fever viruses, reoviruses, orbiviuises, rotaviruses, Hepatitis B virus, parvoviruses, papilloma viruses, polyoma viruses, adenoviruses), herpes simplex virus (HSV) 1 and 2, varicella zoster virus, cytomegalovirus (CMV), variola viruses, vaccinia viruses, pox viruses, African swine fever virus, the unclassified agent of delta hepatitis, the agents of non-A, non-B hepatitis; of infectious bacteria like: Helicobacter pylon, Borrelia burgdorferi, Legionella pneumophila, Mycobacterium tuberculosis, Mycobacterium bovis (BCG), Mycobacterium avium, Mycobacterium intracellulare, Staphylococcus aureus, Neisseria gonorrhoeae, Neisseria meningitidis, Listeria monocytogenes, Streptococcus pyogenes, Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catharralis, Klebsiella pneumoniae, Bacillus anthracis, Corynebacterium diphtheriae, Clostridium perfringers, Clostridium tetani, Enterobacter aerogenes, Klebsiella pneumoniae, Pasturella multocida, and Treponema pallidum; of infectious fungi like: Cryptococcus neoformans, Histoplasma capsulatum, Coccidioides immitis, Blastomyces dermatitidis, Candida albicans; of infectious protists like: Plasmodium falciparum, Trypanosoma cruzi, Leishmania donovani and Toxoplasma gondii. and; of human tumoral cells.
In embodiments of this aspect one or more of the oligonucleotides of this invention and the antigen are administered simultaneously locally (by oral, rectal, intranasal or transdermal route) or systemically (by intradermic or intramuscular injection).
An aspect of this invention is a method of vaccination of a person. The person can be vaccinated prophylactically or therapeutically.
A prophylactic vaccine is designed to elicit protection from a disease caused by an infectious agent through the induction of specific immunity.
A therapeutic vaccine is designed to induce remission of an illness (i.e. a tumor and metastasis or illness associated with an infectious agent like the human immunodeficiency virus).
The method of vaccination includes administering one or more of the oligonucleotides of this invention and one or more antigens—that is, the vaccine can be designed against one disease target or a combination of disease targets.
Another aspect of this invention is a method of treatment of a person with a tumoral disease or an immunological disorder, with one or more of the oligonucleotides of this invention to stimulate his/her endogenous immune response. Examples of tumoral disease are: Chronic Myelogenous Leukemia, Precursor B-lymphoblastic lymphoma, B-cell chronic lymphocytic leukaemia, Lymphoplasmacytic lymphoma, Mantle cell lymphoma, Follicle center lymphoma, (follicular and diffuse), Marginal zone-B lymphoma, Extranodal lymphoma, Nodal marginal zone B-cell lymphoma, Splenic marginal zone B-cell lymphoma, Hairy cell leukaemia, Plasmocytoma, Diffuse large B-cell lymphoma, Burkitt's lymphoma, High grade B-cell lymphoma, Burkift like, Melanoma, Kaposi's Sarcoma, Multiple Myeloma, Renal Cell Carcinoma, Bladder Cancer, Lung Cancer, Skin Cancer, Breast Cancer, Colon Cancer and Uterus Cancer. Examples of immunological disorders are: Allergy, Severe Combined Immunodeficiency, Chronic Granulomatous disease, and Acquired Immunodeficiency Disease.
In embodiments of this aspect, one or more of the oligonucleotide of this invention is/are present in a pharmaceutical formulation that can be liquid or lyophilized in dosage form. Many dosage forms are known in the art and can be applied herein. In embodiments of this aspect one or more of the oligonucleotides of this invention is/are present in the composition at a dose of from about 10 to 10,000 μg per dose. In these preparations one or more of the oligonucleotides of this invention may be combined with other immunostimulant compounds. Examples of well known immunostimulants are: α-interferon, β-interferon, γ-interferon, granulocyte macrophage colony stimulator factor (GM-CSF), interleukin 2 (IL2), interleukin 12 (IL12), CpG oligonucleotides and Mycobacterium bovis BCG cells. Also, one or more of the oligonucleotides of this invention may be combined with an antiinfective or anticancer drug, or a surgical procedure. In all these cases the oligonucleotides of this invention may be administered before, after or simultaneously with the alternative treatment.
Another aspect of this invention is a method of treatment of a person with a tumoral disease or an immunological disorder, contacting lymphocytes or plasmacytoid dendritic cells from the subject with one or more of the oligonucleotides of this invention “ex vivo” and readministering the activated cells to the subject. In embodiments of this aspect one or more of the oligonucleotides of this invention are present in the incubation media in a concentration of about 0.10 to 100 μg per ml.



BRIEF DESCRIPTION OF THE FIGURES
FIG. 1 is a graph plotting the proliferation index of human peripheral blood mononuclear leukocytes (PBMC) cultured with the phosphorothioate ODN IMT 023 (Seq. ID NO 9), the phosphorothioate CpG ODN 2006:









5′ TCGTCGTTTTGTCGTTTTGTCGTT 3′,






the phosphorothioate non-CpG ODN IMT 021 (SEQ ID NO:1). Data represent the mean and standard deviation of five independent assays.

FIG. 2 shows the results from a flow cytometry study using human peripheral blood mononuclear leukocytes (PBMC) to determine the comparative effect of the phosphorothioate CpG ODN 2006:









5′ TCGTCGTTTTGTCGTTTTGTCGTT 3′






and the phosphorothioate non-CpG ODN IMT 021 (SEQ ID NO:1) on activation of the B cell population:

(2a) Representative flow cytometry diagrams comparing B cell activation by IMT 023, 2006 and IMT 021 (2b) Representative histograms of the flow cytometry diagrams showed in (a).
FIG. 3 shows the influence of the position of the immunostimulatory sequence motif (3a) X1X2X3X4X5X6X7X8 here disclosed on the immunostimulatory activity of non-CpG ODNs as measured by proliferation assay (3b) and IL-6 secretion assay (3c). Data represent the mean and standard deviation of three independent assays performed by quadruplicate each.
FIG. 4 shows the induction of CD40 (4a), MHC I (4b) and MHC II (4c) on CD19+ cells (B cells) by non-CpG-ODNs bearing the immunostimulatory sequence motif X1X2X3X4X5X6X7X8 here disclosed. Human PMBC were cultured for 24 hr with indicated ODNs and then stained with fluorescent anti-CD19/anti-CD40 (4a) or, anti-CD19/ anti-MHC I (4b) or, anti-MHC II (4c). Flow cytometric results are presented as histograms. Open histograms correspond to cells cultured in absence of ODN and shaded histograms to cells cultured in presence of ODN. ODN (S) means phosphorothioate ODN.
FIG. 5 shows the induction of CD86 (5a), CD40 (5b) and MHC I (5c) on purified B cells by non-CpG-ODNs bearing the immunostimulatory sequence motif X1X2X3X4X5X6X7X8 here disclosed. Human purified B cells were cultured for 24 hr with indicated ODNs and then stained with fluorescent anti-CD19/anti-CD86 (5a) or, anti-CD19/anti-CD40 (5b) or, anti-CD19/anti-MHC I (5c). Flow cytometric results are presented as histograms. Open histograms correspond to cells cultured in absence of ODN and shaded histograms to cells cultured in presence of ODN. ODN (S) means phosphorothioate ODN.
FIG. 6 shows induction of CD86 (6a), CD40 (6b) and MHC I (6c) in CD19+ cells (B cells) by phosphodiester non-CpG-ODNs bearing the immunostimulatory sequence motif X1X2X3X4X5X6X7X8 here disclosed. Human PMBC were cultured for 48 hr with indicated phosphodiester ODNs and then stained with fluorescent anti-CD19/anti-CD86 (6a) or, anti-CD19/anti-CD40 (6b) or, anti-CD19/anti-MHC I (6c). Flow cytometric results are presented as histograms. Open histograms correspond to cells cultured in absence of ODN and shaded histograms to cells cultured in presence of ODN. ODN (O) means phosphodiester ODN.
FIG. 7 shows stimulation of PMBC proliferation by non-CpG ODNs bearing the motif X1X2X3X4X5X6X7X8 here disclosed in non-human primates. Cebus apella (7a) or Macacca fascicularis PMBC (7b) were cultured for 72 hr with indicated ODNs (6 μg/ml). Data represent the mean and standard deviation of four replicates.
FIG. 8 shows the induction of CD86 (8a), CD40 (8b) and MHC I (8c) on CD 19+ (B cells) of a patient suffering B cell leukemia by non-CpG-ODNs bearing the motif X1X2X3X4X5X6X7X8 here disclosed. PMBC were cultured for 24 hr with indicated ODNs and then stained with fluorescent anti-CD19/anti-CD86 (8a) or, anti-CD19/anti-CD40 (8b) or, anti-CD19/anti-MHC I (8c). Flow cytometric results are presented as histograms. Open histograms correspond to cells cultured in absence of ODN and shaded histograms to cells cultured in presence of ODN. ODN (S) means phosphorothioate ODN.
FIG. 9 shows the induction of CD86 (9a), CD40 (9b) and MHC I (9c) on purified plasmacytoid dendritic cells by non-CpG-ODNs bearing the motif X1X2X3X4X5X6X7X8 here disclosed. More than 95% pure plasmacytoid dendritic cells were cultured for 24 hr with indicated ODNs and then stained with fluorescent anti-CD19/anti-CD86 (9a) or, anti-CD19/anti-CD40 (9b) or, anti-CD19/anti-MHC I (9c). Flow cytometric results are presented as histograms. Open histograms correspond to cells cultured in absence of ODN and shaded histograms to cells cultured in presence of ODN. ODN (S) means phosphorothioate ODN.



DETAILED DESCRIPTION OF THE INVENTION
Definitions
An “allergy” refers to acquired hypersensitivity to a substance (allergen). Examples of allergies are eczema, allergic rhinitis, asthma and urticaria.
An “immune system deficiency” refers to a disease in which the immune system is not functioning in normal capacity.
As used herein, the term “oligonucleotide” or “oligo” shall mean multiple nucleotides (i.e. molecules comprising a sugar (e.g. ribose or deoxyribose) linked to a phosphate group and to an exchangeable organic base, which is either a substituted pyrimidine (e.g. cytosine (C), thymine (T) or uracil (U)) or a substituted purine (e.g. adenine (A) or guanine (G)). The term “oligonucleotide” as used herein refers to both oligoribonucleotides (ORNs) and oligodeoxyribonucleotides (ODNs).
The term “oligonucleotide” shall also include oligonucleosides (i.e. an oligonucleotide minus the phosphate) and any other organic base containing polymer. Oligonucleotides can be obtained from existing nucleic acid sources (e.g. genomic or cDNA), but are preferably synthetic (e.g. produced by oligonucleotide synthesis)
An “oligonucleotide” refers to multiple nucleotides linked by phosphodiester bonds.
An “immunostimulatory oligonucleotide” refers to an oligonucleotide which stimulates (i.e. has a mitogenic effect on, or induces or increases cytokine expression by) a cell of the immune system (ie: a lymphocyte, a macrophage).
A “CpG” refers to an unmethylated cytosine-guanine dinucleotide.
A “CpG oligonucleotide” refers to an oligonucleotide which stimulates a cell of the immune system and its immunostimulatory activity critically depends of the presence of at least one CpG in its sequence.
A “non-CpG oligonucleotide” refers to an oligonucleotide which stimulates a cell of the immune system and its immunostimulatory activity does not critically depends of the presence of a CpG in its sequence.
A “subject” refers to an animal of the order Primate, including humans.
As used herein, the term “treating” refers to a process by which the symptoms of a disease, and more particularly infectious diseases or tumoral diseases or immunological disorders are ameliorated or completely eliminated.
As used herein, the term “preventing” refers to a process by which a disease, and more particularly infectious diseases or tumoral diseases or immunological disorders are obstructed or delayed.
In a preferred embodiment, the immunostimulatory oligonucleotides of the invention are advantageously modified into stabilized oligonucleotides. Such stabilized immunostimulatory oligonucleotide may be particularly useful to obtain a prolonged immunostimulation. As used herein, a “stabilized oligonucleotide” refers to an oligonucleotide that is relatively resistant to in vivo degradation (e.g. via an exo- or endo-nuclease). Preferred stabilized oligonucleotides of the present invention comprise a phosphate backbone modification. More particularly, the phosphate backbone modification is preferably a 5′ inter-nucleotide linkage modification, for instance, at the first two nucleotides of the 5′ end of the oligonucleotide of the invention. Furthermore, the phosphate backbone modification may be a 3′ inter-nucleotide linkage modification. In such a case, the modification may occur, for instance, at the last two nucleotides of the 3′ end of the oligonucleotide of the invention. Even more preferably, the immunostimulatory oligonucleotide of the invention may be stably modified so as to comprise a phosphorothioate-linked nucleotide (i.e. at least one of the phosphate oxygens is replaced by sulfur). In the most preferred embodiment, most if not all the nucleotides of the immunostimulatory oligonucleotides of the invention comprise a phosphorothioate-linked nucleotide.
Other stabilized oligonucleotides may alternatively include: nonionic DNA analogs, such as alkyl- and aryl-phosphonates (in which the charged phosphonate oxygen is replaced by an alkyl or aryl group), phosphodiester and alkylphosphotriesters, in which the charged oxygen moiety is alkylated. Oligonucleotides which contain a diol, such as tetraethyleneglycol or hexaethyleneglycol, at either or both termini have also been shown to be substantially resistant to nuclease degradation.
Description
The present invention provides methods to augment the immune response of animals of the order Primate, including humans, adding to vaccines one or more of the oligonucleotides of this invention or performing a treatment based in the administration of one or more of the oligonucleotides of this invention to a person with a tumoral disease or an immunological disorder or contacting white blood cells obtained from a person with a tumoral disease or an immunological disorder with one or more of the oligonucleotides of this invention “ex vivo”, and readministering these activated white blood cells to the same person.
Vaccines compositions useful containing one or more of the oligonucleotides of this invention can present antigens directly (i.e., in the form of a defined protein or polysaccharide) or as a part of a complex biological entity (i.e. complete viruses; complete bacterial cells; bacterial membranes or artificial conjugates like polysaccharide-protein conjugates). These antigens can be combined in multiple vaccines.
A vaccine composition including at least one antigen is formulated to include one or more of the oligonucleotides of this invention.
For example the antigen can be Moraxella catharralis killed cells or subcellular fractions of these cells or Hepatitis B virus surface antigen natural or produced by means of the DNA recombinant technology.
One or more of the oligonucleotides of this invention may be formulated alone or together with one or more antigens in a pharmaceutical composition, which may also include carriers, thickeners, diluents, buffers, preservatives, surface active agents, anti-microbial agents, anti-inflammatory agents, anesthetics and the like.
The formulation can be liquid or lyophilized. The pharmaceutical composition may be administered in a number of ways depending of whether local or systemic treatment is desired, and on the area to be treated. Administration may be done topically, orally, by inhalation or parenterally. Formulation for topical administration may include ointments, lotions, creams, gels, drops, suppositories, sprays, liquids and powders. Pharmaceutical carriers, aqueous, powder or oily bases, thickeners and the like may be necessary or desirable. Formulations for oral administration include powders or granules, suspensions or solutions in water or non-aqueous media, capsules, tablets and the like. Thickeners, flavorings, diluents, emulsifiers and the like may be necessary or desirable. Formulations for parenteral administration include sterile aqueous solutions, which may also contain buffers, diluents and other additives. A vaccine containing one or more antigens and one or more of the oligonucleotides of this invention can be formulated and used for prophylactic or therapeutic purposes.
Common antigens used in viral prophylactic vaccines are from Hepatitis B virus, Hepatitis A virus and Influenza virus. Common antigens used in bacterial prophylactic vaccines are from Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catharralis, Klebsiella pneumoniae and Mycobacterium bovis (BCG). Common antigens used in therapeutic vaccines are from Papilloma virus, HIV virus and melanoma cells.
A further refinement of a vaccine formulation is to incorporate one or more of the oligonucleotides of this invention as adjuvant/s and the antigen/s into a delivery vehicle to provide for delayed release of the active compounds of the vaccine over time. This can be accomplished by various means known in the art. Examples of these means are encapsulation into Poly (lactide-coglicolide) micro particles (Kersten, G. F. A. and Gander, B. 1996. Biodegradable Micro Spheres as vehicles for antigens, in: S. H. E. Kaufmann, ed. Concepts in Vaccine Development. Walter de Gruyter. Berlin-New York), liposomes (Gregoriadis, G. et al. 2000. Liposomes as Immunological Adjuvants and Vaccine Carriers, in: S. H. E. Kaufmann, ed. Concepts in Vaccine Development. Walter de Gruyter. Berlin-New York) and poly (methyl methacrylate) nanoparticles (Kreuter, J. 2000. Poly (Methyl Methacrylate) nanoparticles as vaccine adjuvants, in: S. H. E. Kaufmann, ed. Concepts in Vaccine Development. Walter de Gruyter. Berlin-New York).
Another refinement of the vaccine formulation is to conjugate the antigen/s and one or more of the oligonucleotides of this invention, by chemical means (Mier W, Eritja R, Mohammed A, Haberkorn U, Eisenhut M. 2000. Preparation and evaluation of tumor-targeting peptide-oligonucleotide conjugates. Bioconjug. Chem. 11:855).
Many vaccine formulations are known in the art and can be used by substituting one or more of the oligonucleotides of this invention for the adjuvant previously known or by simply adding one or more of the oligonucleotides of this invention to the original formulation.
Based on their immunostimulatory properties, one or more of the oligonucleotides of this invention can also be administered to a subject in vivo to treat a tumoral disease or an immune system disorder.
Examples of common tumoral diseases are: Chronic Myelogenous Leukemia, Melanoma, Kaposi's Sarcoma, Multiple Myeloma, Renal Cell Carcinoma, Bladder Cancer, Lung Cancer, Skin Cancer, Brest Cancer, Colon Cancer and Uterus Cancer. Examples of common immunological disorders are: Allergy, Severe Combined Immunodeficiency, Chronic Granulomatous disease, and Acquired Immunodeficiency Disease.
The pharmaceutical composition for these treatments may include one or more of the oligonucleotides of this invention together with carriers, thickeners, diluents, buffers, preservatives, surface active agents, anti-microbial agents, anti-inflammatory agents, anesthetics and the like. The formulation can be liquid or lyophilized.
The pharmaceutical composition may be administered in a number of ways depending of whether local or systemic treatment is desired, and on the area to be treated. Administration may be done topically, orally, by inhalation or parenterally. Formulation for topical administration may include ointments, lotions, creams, gels, drops, suppositories, sprays, liquids and powders. Pharmaceutical carriers, aqueous, powder or oily bases, thickeners and the like may be necessary or desirable. Formulations for oral administration include powders or granules, suspensions or solutions in water or non-aqueous media, capsules, tablets and the like. Thickeners, flavorings, diluents, emulsifiers and the like may be necessary or desirable. Formulations for parenteral administration include sterile aqueous solutions, which may also contain buffers, diluents and other additives. Alternatively, one or more of the oligonucleotides of this invention can be contacted with immunocompetent cells (i.e. B cells or plasmacytoid dendritic cells) obtained from a subject having a tumoral disease or an immune system deficiency “ex vivo” and activated cells can then be reintroduced in the subject.
EXAMPLE 1 
Materials and Methods
The following materials and methods were used generally throughout the examples.
1) Oligonucleotides
Oligonucleotides having phosphorothioate internucleotide linkages were purchased, purified by high-pressure liquid chromatography (HPLC), from Operon Technologies (Alameda, Calif.) or Annovis (Aston, Pa.) or Oligos Etc (Bethel, Me.). Oligonucleotides were suspended in depyrogenated water and kept at −20° C. until used.
2) Antibodies
Antibodies used in assays were purchased from Serotec (Raleigh, N.C.).
3) Peripheric Blood Mononuclear Leukocytes (PBMC)
Blood was obtained by venipuncture from healthy donors using heparin as anticoagulant. PMBC were isolated by Ficoll-Hypaque (Sigma Diagnostics Inc., St. Louis, Mo.) density gradient centrifugation. Briefly, blood samples diluted 1:2 in RPMI-1640 medium (PAA laboratories GmbH, Linz, Austria) supplemented with 2.0 mM L-glutamine and 50.0 μg/ml gentamicin and 20 mM HEPES were centrifuged at 1000×g for 40 minutes at 20° C. PMBC were isolated, washed and suspended in medium supplemented with 10% fetal calf serum.
4) Purification of Cells
B lymphocytes and plasmacytoid dendritic cells were purified from human PMBC by positive selection using the MACS magnetic cell separation systems (Miltenyi Biotec, Germany).
5) Cell Proliferation Assays
Blood was obtained by venipuncture from healthy donors using heparin as anticoagulant. PMBC were isolated by Ficoll-Hypaque (Sigma Diagnostics Inc., St. Louis, Mo.) density gradient centrifugation. Briefly, blood samples diluted 1:2 in RPMI-1640 medium (PAA laboratories GmbH, Linz, Austria) supplemented with 2.0 mM L-glutamine and 50.0 μg/ml gentamicin and 20 mM HEPES were centrifuged at 1000×g for 40 minutes at 20° C. PMBC were isolated, washed and suspended in medium supplemented with 10% fetal calf serum.
6) IL6 Assay
PBMC (3×105/well) were cultured as described above with ODNs (6 μg/ml) for 24 hr. After this, supernatants were collected and IL6 levels measured by ELISA. Briefly, 96 well micro titer plates (NUNC, Denmark) were coated with anti-IL6 antibodies and blocked with RPMI 1640 media supplemented with 10% (v/v) heat inactivated FCS. IL6 was detected calorimetrically using biotin-labeled antibodies followed by peroxidase-conjugated strepto-avidin and then peroxidase-specific colorimetric substrate. Standard curves were generated using known amounts of recombinant IL6. The detection limit of these assays was 30 pg/ml. All assays were performed in duplicate.
6) IgM Secretion Assay
PBMC (3×105/well) were cultured as described above with ODNs (1.5 μg/ml) for 72 hr. After this, supernatants were collected and IgM assayed by ELISA. Briefly, 96 microtiter plates (NUNC, Denmark) were coated with anti-IgM antibodies and blocked with RPMI 1640 media. IgM was detected calorimetrically using peroxidase-labeled antibodies followed by peroxidase-specific calorimetric substrate. Standard curves were generated using known amounts of purified IgM. The detection limit of these assays was 50 ng/ml. All assays were performed in duplicate.
6) Flow Cytometry
Staining of surface antigens was performed as described (J. Fló and E. Massouh. Age-related changes of native and memory CD4 rat lymphocyte subsets in mucosal and systemic lymphoid organs. Developmental and comparative Immunology 21: 443-453, 1997). Anti CD19 (Clone LT19), CD86 (Clone BU63), CD40 (clone LOB 7/6), CD4 (clone S 3.5), MHC class I (Clone W6/32) and MHC class II (Clone WR 18) antibodies were purchased from Serotec (Raleigh, N.C., USA).
Flow cytometric data of 10,000 cells/sample were acquired on a FACScan (Becton Dickinson Immunocytometry Systems, San Jose, Calif.). Data were analyzed using the computer program Win MDI, 2.8, Interface Flow Cytometry Application (Joseph Trotter Copyright 1993-1998).
7) Immunization of Monkeys Against Hepatitis B Surface Antigen (HBsAg) and Evaluation of the Humoral Response
Twelve monkeys of the species Cebus Apella (2.5-3.5 Kg) were immunized with a pediatric dose of AgB (Pablo Cassará, Buenos Aires, Argentina) containing 10 μg of HBsAg adsobed to alumina (25 mg of Al3+/mg of HBsAg). This was administered alone (n=3, 1 female, 2 males) or combined with indicated phosphorothioate ODN (150 μg/dose) (n=3, 1 female, 2 males). All vaccines were administered intramuscular (i.m.) in the quadriceps muscle in a total volume of 1 ml. Monkeys were maintained in the animal facility of the CEMIC (Centro Medico de Investigaciones Clinicas), Buenos Aires, Argentina. Animals were monitored daily by specialists and weighed once per week. Plasma was recovered by intravenous (i.v.) puncture before and at various times after immunization and immediately assayed for antibodies using the commercial kit AUSAB (Abbot Laboratories, Illinois, USA). Titers are expressed in milliinternational units per ml.
EXAMPLE 2 
Selection of Oligonucleotide Sequences
WO 96/02555 and U.S. Pat. No. 6,239,116 patents teach that to be immunostimulatory, oligonucleotides require sequences containing unmethylated CpG motifs (WO 96/02555, col. 13 lines 19-20 and U.S. Pat. No. 6,239,116, col. 6 lines 1-3). EP 0 468 520 teaches that to be immunostimulatory, oligonucleotides require a palindromic sequence of at least 6 nucleotides long to be satisfactory (EP 0 468 520, col. 11, lines 34-37). Therefore, several non-CpG oligonucleotides, without palindromic sequences of at least 6 nucleotides long were probed using proliferation assays, cell differentiation assays, cytokine IL6 secretion assays and IgM secretion assays performed on human peripheral blood mononuclear leukocytes (PBMC). As a positive control, the CpG oligonucleotide 2006 of composition:









5′ TCGTCGTTTTGTCGTTTTGTCGTT 3′






and phosphorothioate bonds described by Hartman and Krieg (Hartmann, G., Krieg, A. M. 2000. Mechanism and function of a newly identified CpG DNA motif in human primary B cells. J. Immunol. 164:944.) was used. As a background control the phosphorothioate oligonucleotide IMT 023 (SEQ ID NO9) or IMT 022 (SEQ ID NO8) with very low activity on human cells were used.

Also as a “presumably” negative control an oligonucleotide with the same composition of the oligonucleotide 2006 but in which all the CpG dinucleotides have been replaced by GpC dinucleotides:









5′ TGCTGCTTTTGTGCTTTTGTGCTT 3′






was used. This oligonucleotide was named ODN IMT 021 (SEQ ID NO 1). FIG. 1 shows a proliferation assay performed with the above described oligonucleotides.

It was found, surprisingly, that the non CpG oligonucleotide IMT 021 was as active as the 2006 CpG oligonucleotide in proliferation assays (FIG. 1 and Table 1) if used at 1.5 mug/ml and approximately 40-60% active if used at 0.375 mug/ml.








TABLE 1







Induction of peripheral white blood cell proliferation by



Phosphorothioate CpG (2006) and non-CpG oligonucleotides


(IMT 021)










PROLIFERATION INDEX












(ODN at
(ODN at




1.5 μg/ml)
0.375 μg/ml)














ODN (S)
SEQUENCE (5′–3′)
Avg.
N
SD
Avg.
N
SD


















2006
TCGTCGTTTTGTCGTTTTGTCGTT
17.95
4
2.39
13.83
14
1.55



 


IMT 021
TGCTGCTTTTGTGCTTTTGTGCTT
17.41
5
2.74
8.02
15
1.21





IMT 021 (SEQ. ID No 1)


Avg.: Average;


N: Number of Data;


SD: Standard Deviation;


ODN(S): phosphorothioate ODN






Immunostimulation was also evaluated by Flow Cytometry using the CD 19 general marker for human B cells and the CD 86 activation marker for human B cells.
FIG. 2 shows the activation of human B cells incubated with the non CpG ODN (S) IMT 021 as compared to the activation induced by incubation with the CpG ODN (S) 2006. As can be observed, both ODNs show similar activation pattern as compared with the ODN(S) IMT 023 background control.
IL6 secretion was also evaluated in supernatants of PBMC incubated with the CpG ODN (S) 2006 and the non CpG ODN (S) IMT 021 (Table 2).







TABLE 2







Induction of human IL-6 secretion by phosphorothioate


CpG and non-CpG oligonucleotides 2006 and IMT 021










IL-6 (pg/ml)














ODN (S)
expt. 1
expt. 2
expt. 3
expt. 4

















cells alone
0
0
0
0



2006
568
159
597
374



IMT 021
348
384
836
596







IMT 021 (SEQ. ID NO 1)






Results of this assay also indicate that the non-CpG ODN (S) IMT 021 is an effective immunostimulant. Therefore, other non-CpG sequence variants of the 2006 oligonucleotide were investigated. Table 3 shows the results for six of these variants in which, the Cs or Gs of all the CpGs of this ODN were replaced by other nucleotides. As can be observed, the Gs of the CpGs in the ODN(S) 2006 are not necessary for B cell proliferation or IL6 secretion. However, modification of the Cs of the CpGs is detrimental if the replacement is for As or Gs but not if it is for Ts. These results clearly indicate that stimulation of the B cell proliferation and IL6 secretion by the ODN(S) 2006 is not at all associated with integrity of the CpG group.








TABLE 3







Induction of peripheral white blood cell proliferation and IL6



secretion by non-CpG variants of the ODN(S) 2006 oligonu-


cleotide











PROLIFERATION





INDEX




(ODN at
IL-6 (pg/ml)



0.375 μg/ml)
(ODN at 6.0 μg/ml)














ODN (S)
SEQUENCE (5′–3′)
Avg.
N
SD
Avg.
N
SD


















2006
TCGTCGTTTTGTCGTTTTGTCGTT
13.83
14
1.55
215.8
4
83.4



 


IMT 504
TCATCATTTTGTCATTTTGTCATT
13.08
2
3.80
285.2
2
39.5


 


IMT 505
TCCTCCTTTTGTCCTTTTGTCCTT
12.98
2
4.73
218.9
2
3.1


 


IMT 506
TCTTCTTTTTGTCTTTTTGTCTTT
11.66
4
2.77
228.9
4
161.0


 


IMT 501
TAGTAGTTTTGTAGTTTTGTAGTT
5.42
2
2.03
66.8
2
54.0


 


IMT 502
TGGTGGTTTTGTGGTTTTGTGGTT
7.16
2
3.24
89.7
2
91.4


 


IMT 503
TTGTTGTTTTGTTGTTTTGTTGTT
9.87
2
1.90
334.9
2
215.5





IMT 504 (SEQ ID No 2);


IMT 505 (SEQ ID No 3);


IMT 506 (SEQ ID No 4);


IMT 501 (SEQ ID No 5);


IMT 502 (SEQ ID No 6);


IMT 503 (SEQ ID No 7)






EXAMPLE 3 
Effect of Structure Modification on the Immunostimulatory Activity of Non-CPG Oligonucleotides: Definition of the Active Motif
In order to study the influence of the primary structure on the immunostimulatory activity of the non-CpG ODNs, several variants of the 2006 and IMT 021 oligonucleotides were synthesized. Table 4 shows the primary structure of some of the IMT 021 variants and the results of a proliferation and IL-6 assays performed in order to evaluate its immunostimulatory activity.
The ODN(S) IMT 021 contains T in positions 7,8,9,10,12,15,16,17,18,20,23 and 24. Replacement of these Ts with As (ODN IMT 022) or Cs (ODN IMT 023) results in a very significant loss of activity (about 76% and 75% respectively) in the proliferation assay and also in the IL-6 secretion assay (84% and 88% respectively). These results indicate that some or all the Ts in positions 7,8,9,10,12,15,16,17,18,20,23 and 24 are critical for the ODN IMT 021 immunostimulatory activity.








TABLE 4







Induction of peripheral white blood cell proliferation and



IL-6 secretion by Phosphorothioate non-CpG oligonucleotides


derived from the inmunostimulatory IMT 021 oligonucleotide











PROLIFERATION





INDEX




(ODN at
IL-6 (pg/ml)



0.375 μg/ml)
(ODN at 6.0 μg/ml)














ODN (S)
SEQUENCE (5′–3′)
Avg.
N
SD
Avg.
N
SD


















IMT 021
TGCTGCTTTTGTGCTTTTGTGCTT
8.02
15
1.21
181.3
4
49.9



 


IMT 022
TGCTGCAAAAGAGCAAAAGAGCAA
1.24
2
0.30
0.0
2
0.0


 


IMT 023
TGCTGCCCCCGCGCCCCCGCGCCC
1.07
4
0.12
0.0
3
0.0





IMT 021 (SEQ. ID No 1);


IMT 022 (SEQ. ID No 8);


IMT 023 (SEQ. ID No 9)






The analysis of hundreds of ODNs (unshown) allowed definition of a core sequence (motif) responsible of the immunostimulatory activity as measured by B cell proliferation and IL6 secretion. This motif is the following:

X1X2X3X4X5X6X7X8,

wherein X1 is C,T,G or A (preferably T or C);
wherein X2 is C,T,G or A;
wherein X7 is C,T,G or A (preferably G);
wherein at least three, and preferably all, of X3, X4, X5, X6 and X8 are T.
Table 5 shows the effect of changes in each of the nucleotides of the two non-CpG motifs present in the ODN IMT 504 (motif: CATTTTGT). Replacement of the C in position 1 of the motif by A (ODN IMT 531) or G (ODN IMT 533) resulted in a loss of about 50% in the activity. However, replacement of this C by T (ODN IMT 532) does not change the activity. Thus, in order to obtain maximal activity the first position of the motif should be occupied by a pyrimidine nucleotide (C or T). In position 2, A (ODN IMT 504) or T (ODN IMT 535) or C (ODN IMT 534) are equivalent options.
In order to study the influence of a G nucleotide in position 2 of the motif without introduction of a CpG dinucleotide, ODNs with a T in the first position of the motif were synthesized (Table 6). As can be observed, any nucleotide in the second position of the immunostimulatory motif is equivalent.
Replacement of the G in position 7 of the immunostimulatory motif by A (ODN IMT 541), T (ODN IMT 542) or C (ODN IMT 543) is detrimental (Table 5). Thus, in order to obtain maximal activity position 7 should be preferably G.
Regarding to the positions of the immunostimulatory motif occupied by Ts, the most sensitive (as measured by a change for an A) are 4 (ODN IMT 538), 5 (ODN ITM 539) and 8 (ODN IMT 544) and less sensitive are 3 (ODN IMT 537) and 6 (ODN IMT 540). Replacement of two or more of the Ts within the motif results in more than 70% loss of activity (ODNs IMT 545, IMT 546, IMT 547 ,IMT 548, IMT 549, IMT 550, IMT 551 and IMT 552). Therefore, in order to obtain a significant immunostimulatory activity three or more of positions 3,4,5,6 and 8 should be occupied by T.








TABLE 5







Induction of peripheral white blood cell proliferation and



IL-6 secretion by phosphorothioate non-CpG oligonucleotides


of this invention derived from ODN IMT 504











PROLIFERATION





INDEX




(ODN at
IL-6 (pg/ml)



0.375 μg/ml)
(ODN at 6.0 μg/ml)














ODN (S)
SEQUENCE (5′–3′)
Avg.
N
SD
Avg.
N
SD


















IMT 022
TGCTGCAAAAGAGCAAAAGAGCAA
1.34
24
0.52
42.7
6
29.7



 


IMT 504
TCATCATTTTGTCATTTTGTCATT
13.85
16
4.36
171.6
6
39.8


 


IMT 531
TCATAATTTTGTAATTTTGTCATT
7.59
12
2.71
137.6
6
124.4


 


IMT 532
TCATTATTTTGTTATTTTGTCATT
13.80
12
3.41
124.2
6
78.4


 


IMT 533
TCATGATTTTGTGATTTTGTCATT
6.20
12
2.71
118.3
6
76.0


 


IMT 534
TCATCCTTTTGTCCTTTTGTCATT
11.67
12
4.49
173.7
6
31.4


 


IMT 535
TCATCTTTTTGTCTTTTTGTCATT
12.49
12
2.51
160.6
6
39.0


 


IMT 537
TCATCAATTTGTCAATTTGTCATT
10.17
12
4.00
183.5
6
63.6


 


IMT 538
TCATCATATTGTCATATTGTCATT
7.12
12
2.95
133.2
6
86.7


 


IMT 539
TCATCATTATGTCATTATGTCATT
9.80
12
4.22
171.7
6
53.4


 


IMT 540
TCATCATTTAGTCATTTAGTCATT
11.94
12
2.55
163.1
6
43.0


 


IMT 541
TCATCATTTTATCATTTTATCATT
9.30
12
2.20
177.2
6
40.4


 


IMT 542
TCATCATTTTTTCATTTTTTCATT
9.78
12
2.60
151.1
6
40.2


 


IMT 543
TCATCATTTTCTCATTTTCTCATT
4.75
12
2.25
137.9
6
77.6


 


IMT 544
TCATCATTTTGACATTTTGACATT
7.16
12
2.11
124.4
6
42.7


 


IMT 545
TCATCATTTAGACATTTAGACATT
4.19
12
1.81
129.4
6
53.8


 


IMT 546
TCATCATTATGACATTATGACATT
2.90
12
1.16
105.0
6
67.2


 


IMT 547
TCATCATATTGACATATTGACATT
3.34
12
1.16
110.3
6
43.5


 


IMT 548
TCATCATTAAGACATTAAGACATT
3.40
12
1.53
91.5
6
33.1


 


IMT 549
TCATCATATAGACATATAGACATT
3.51
12
1.03
121.3
6
41.3


 


IMT 550
TCATCATAATGACATAATGACATT
1.91
12
0.71
79.3
6
30.8


 


IMT 551
TCATCATAAAGACATAAAGACATT
2.17
12
0.72
60.7
6
26.4


 


IMT 552
TCATCAAAAAGACAAAAAGACATT
1.37
12
0.83
21.6
6
13.5





IMT 022 (SEQ. ID No 8);


IMT 504 (SEQ ID No 2);


IMT 531 (SEQ ID No 14);


IMT 532 (SEQ ID No 15);


IMT 533 (SEQ ID No 16);


IMT 533 (SEQ ID No 17);


IMT 534 (SEQ ID No 18);


IMT 535 (SEQ ID No 19);


IMT 537 (SEQ ID No 30);


IMT 538 (SEQ ID No 31);


IMT 539 (SEQ ID No 32);


IMT 540 (SEQ ID No 33);


IMT 541 (SEQ ID No 34);


IMT 542 (SEQ ID No 35);


IMT 543 (SEQ ID No 36);


IMT 544 (SEQ ID No 37);


IMT 545 (SEQ ID No 38);


IMT 546 (SEQ ID No 39);


IMT 547 (SEQ ID No 40);


IMT 548 (SEQ ID No 41);


IMT 549 (SEQ ID No 42);


IMT 550 (SEQ ID No 43);


IMT 551 (SEQ ID No 44);


IMT 552 (SEQ ID No 45)














TABLE 6







Induction of peripheric white blood cell proliferation and



IL-6 secretion by Phosphorothioate non-CpG oligonucleotides


with T in the first position of the immunostimulatory motif











PROLIFERATION





INDEX




(ODN at
IL-6 (pg/ml)



0.375 μg/ml)
(ODN at 6.0 μg/ml)














ODN (S)
SEQUENCE (5′–3′)
Avg.
N
SD
Avg.
N
SD


















IMT 532
TCATTATTTTGTTATTTTGTCATT
10.10
4
1.61
221.7
4
35.3



 


IMT 197
TCATTTTTTTGTTTTTTTGTCATT
10.84
4
1.06
386.1
4
123.3


 


IMT 198
TCATTGTTTTGTTGTTTTGTCATT
10.00
4
1.75
235.3
4
27.1


 


IMT 199
TCATTCTTTTGTTCTTTTGTCATT
12.36
3
1.71
235.0
4
6.0





IMT 532 (SEQ ID No 15);


IMT 197 (SEQ. ID No 10);


IMT 198 (SEQ ID No 11);


IMT 199 (SEQ ID No 12)






To investigate the effect of changes in the position of the motif within the ODN chain, the non-CpG motif CATTTTGT present in ODN IMT504 was introduced into different locations in a 24 nucleotides long poly T chain (FIG. 3). As can be seen, the poly T ODN (S) by itself has a significant mitogenic activity. On the other hand, introduction of only one CATTTTGT motif results in an increment of 1.2 to 1.8 times as measured by proliferation assays, or 2.4 to 3.5 times as measured by IL-6 secretion assays, in the immunostimulatory activity of the polyT chain depending on where the motif is located. A distance of at least two nucleotides from the 5′ end or four from the 3′ end seems to be necessary in order to reach maximal activity (ODNs IMT174 to IMT 179). Introduction of two motifs in optimal positions (ODN IMT182) results in an increment of 1.9 times as measured by proliferation assays, or 2.9 times as measured by IL-6 secretion assays, in the immunostimulatory activity of the polyT chain. This indicate that contribution of the second motif is negligible. On the other hand, the activity of the most effective ODNs like ODN IMT504 is more than 3 times larger than the activity of the poly T as measured by proliferation assays a fact that suggest that there is also a significant influence of the composition of at least some of the nucleotides surrounding the motif of this invention, in the overall activity of the ODN.
EXAMPLE 4 
Effect of Structure Modification on the Immunostimulatory Activity of Non-CpG Oligonucleotides of This Invention: Influence of the Nucleotide Composition Outside of the Active Motif
Results shown in FIG. 3 indicate that composition of the oligonucleotide outside the non-CpG core motif is important in order to reach optimal immunostimulatory activity. Therefore, a number of oligonucleotides were synthesized with changes in the composition of the nucleotides surrounding the motifs. Table 7 shows the effect of changes in the composition of the first four nucleotides of the 5′ end and of the last four nucleotides the 3′ end in the immunostimulatory activity of ODN IMT 504. As can be observed, the best choices are: C or G in position −1 respect to the two motifs, C, T or G in position −2, any nucleotide in positions −3 and −4, A or T in position +1, G in position +2, any nucleotide in positions +3 and G is the best choice in position +4 .
Of course, other nucleotide combinations not represented in this table may have equal or even better effect on the activity of the non CpG immunostimulatory oligonucleotides. One of ordinary skill in the art can empirically determine other effective combinations.








TABLE 7







Induction of peripheric white blood cell proliferation by



non- CpG ODN(S) IMT 504 with variations in the composi-


tion outside the two immunostimulatory motifs











PROLIFERATION





INDEX
IL-6 (pg/ml)



(ODN at
(ODN at



0.375 μg/ml)
6.0 μg/ml)














ODN (S)
SEQUENCE (5′–3′)
Avg.
N
SD
Avg.
N
SD


















IMT 552
TGCTGCAAAAGAGCAAAAGAGCAA
1.9
12
0.7
<39.9
4
—



 


IMT 504
TCATCATTTTGTCATTTTGTCATT
11.0
8
0.9
171
2
14


 


IMT 559
ACATCATTTTGTCATTTTGTCATT
13.4
4
1.0
128
3
3


 


IMT 560
CCATCATTTTGTCATTTTGTCATT
12.1
4
0.9
145
4
32


 


IMT 561
GCATCATTTTGTCATTTTGTCATT
9.5
4
1.1
209
4
74


 


IMT 562
TAATCATTTTGTCATTTTGTCATT
12.4
4
1.5
171
4
24


 


IMT 563
TTATCATTTTGTCATTTTGTCATT
11.1
3
0.7
172
4
63


 


IMT 564
TGATCATTTTGTCATTTTGTCATT
12.8
4
0.9
118
4
38


 


IMT 565
TCCTCATTTTGTCATTTTGTCATT
14.1
4
0.5
135
4
25


 


IMT 566
TCTTCATTTTGTCATTTTGTCATT
13.9
4
2.2
157
4
28


 


IMT 567
TCGTCATTTTGTCATTTTGTCATT
12.9
4
0.7
259
4
25


 


IMT 568
TCAACATTTTGTCATTTTGTCATT
10.3
4
1.1
153
4
35


 


IMT 569
TCACCATTTTGTCATTTTGTCATT
15.0
4
1.2
199
3
12


 


IMT 570
TCAGCATTTTGTCATTTTGTCATT
12.5
4
0.4
181
3
2


 


IMT 571
TCATCATTTTGTCATTTTGTAATT
14.2
4
0.7
163
4
26


 


IMT 572
TCATCATTTTGTCATTTTGTTATT
13.2
4
1.4
182
3
67


 


IMT 573
TCATCATTTTGTCATTTTGTGATT
8.7
4
0.7
177
4
29


 


IMT 574
TCATCATTTTGTCATTTTGTCCTT
8.1
4
0.7
179
3
58


 


IMT 575
TCATCATTTTGTCATTTTGTCTTT
10.8
4
0.8
150
3
47


 


IMT 576
TCATCATTTTGTCATTTTGTCGTT
12.6
4
1.1
202
4
55


 


IMT 577
TCATCATTTTGTCATTTTGTCAAT
10.0
4
1.4
243
3
8


 


IMT 578
TCATCATTTTGTCATTTTGTCACT
10.9
4
0.9
213
2
9


 


IMT 579
TCATCATTTTGTCATTTTGTCAGT
10.7
4
1.7
182
4
18


 


IMT 580
TCATCATTTTGTCATTTTGTCATA
11.7
4
1.4
194
4
36


 


IMT 581
TCATCATTTTGTCATTTTGTCATC
10.7
4
1.0
173
4
18


 


IMT 582
TCATCATTTTGTCATTTTGTCATG
12.1
4
0.8
242
3
231





IMT 552 (SEQ. ID No 45);


IMT 504 (SEQ ID No 2);


IMT 559 (SEQ ID No 46);


IMT 560 (SEQ. ID No 47);


IMT 561 (SEQ ID No 48);


IMT 562 (SEQ ID No 49);


IMT 563 (SEQ ID No 50);


IMT 564 (SEQ ID No 51);


IMT 565 (SEQ ID No 52);


IMT 566 (SEQ ID No 53);


IMT 567 (SEQ ID No 54);


IMT 568 (SEQ ID No 55);


IMT 569 (SEQ ID No 56);


IMT 570 (SEQ ID No 57);


IMT 571 (SEQ ID No 58);


IMT 572 (SEQ ID No 59);


IMT 573 (SEQ ID No 60);


IMT 574 (SEQ ID No 61);


IMT 575 (SEQ ID No 62);


IMT 576 (SEQ ID No 63);


IMT 577 (SEQ ID No 64);


IMT 578 (SEQ ID No 65);


IMT 578 (SEQ ID No 66);


IMT 580 (SEQ ID No 67);


IMT 581 (SEQ ID No 68);


IMT 582 (SEQ ID No 69)






EXAMPLE 5
Effect of Structure Modification on the Immunostimulatory Activity of Non-CpG Oligonucleotides of This Invention: Influence of the Size of the Oligonucleotide
Table 8 shows that ODNs with one immunostimulatory motif are active if the chain is 16 or more nucleotides long. Activity is maximal if the ODN is 20 or more nucleotides long.








TABLE 8







Effect of the phosphorothioate non-CpG oligonucleotides



size on B cells proliferation and IL6 secretion











PROLIFERATION





INDEX
IL-6 (pg/ml)



(ODN at
(ODN at



0.375 μg/ml)
6.0 μg/ml)














ODN (S)
SEQUENCE (5′–3′)
Avg.
N
SD
Avg.
N
SD


















IMT 187
TTTTCATTTTGT
0.56
8
0.20
<50
4




 


IMT 188
TTTTCATTTTGTTTTT
2.76
8
1.73
147
4
40


 


IMT 189
TTTTCATTTTGTTTTTTTTT
8.21
8
3.19
227
4
21


 


IMT 175
TTTTCATTTTGTTTTTTTTTTTTT
8.92
8
1.80
220
4
18


 


IMT 179
TTTTTTTTTTTTCATTTTGTTTTT
6.06
8
2.00
224
4
82


 


IMT 191
TTTTCATTTTGTTTTTTTTTTTTTTTTT
6.64
8
2.37
205
4
15





IMT 187 (SEQ ID No 70);


IMT 188 (SEQ ID No 71);


IMT 189 (SEQ ID No 72);


IMT 179 (SEQ ID No 73);


IMT 191 (SEQ ID No 77)






EXAMPLE 6 
Induction of Peripheric White Blood Cell IgM Secretion by Phosphorothioate Non-CpG Oligonucleotides of This Invention
Induction of IgM secretion in peripheral white blood cells is another important marker of immunostimulatory activity of oligonucleotides. Table 9 shows the stimulation of IgM secretion by several of the phosphorothioate non-CpG oligonucleotides described above. As can be observed, the most active non-CpG ODN(S)s in induction of proliferation and IL6 secretion are also the best in induction of IgM secretion.








TABLE 9







Induction of peripheral white blood cell IgM



secretion by phosphorothioate non-CpG oligo-


nucleotides of this invention










IgM SECRETION




(ng/ml)



(ODN at



1.50 μg/ml)











ODN (S)
SEQUENCE (5′–3′)
Avg.
N
SD















cells

76
9
50



alone


 


2006
TCGTCGTTTTGTCGTTTTGTCGTT
778
47
204


 


IMT 021
TGCTGCTTTTGTGCTTTTGTGCTT
850
25
264


 


IMT 501
TAGTAGTTTTGTAGTTTTGTAGTT
585
25
179


 


IMT 502
TGGTGGTTTTGTGGTTTTGTGGTT
555
19
277


 


IMT 503
TTGTTGTTTTGTTGTTTTGTTGTT
640
19
220


 


IMT 504
TCATCATTTTGTCATTTTGTCATT
912
24
244


 


IMT 505
TCCTCCTTTTGTCCTTTTGTCCTT
664
23
336


 


IMT 506
TCTTCTTTTTGTCTTTTTGTCTTT
751
43
237


 


IMT 509
AAAAAACTAAAAAAAACTAAAAAA
195
17
77





IMT 021 (SEQ. ID No 1);


IMT 504 (SEQ ID No 2);


IMT 505 (SEQ ID No 3);


IMT 506 (SEQ ID No 4);


IMT 501 (SEQ ID No 5);


IMT 502 (SEQ ID No 6);


IMT 503 (SEQ ID No 7);


IMT 509 (SEQ ID No 13)






EXAMPLE 7 
Stimulation of the Expression of CD40, MHC I and MHC II in B Lymphocytes by Phosphorothioate Non-CPG Oligonucleotides of This Invention
As previously shown (FIG. 2), the non CpG ODN IMT 021 is able to stimulate the expression of CD86 on CD19+ cells (B lymphocytes). In order to extent this observation to other important cell surface markers, human PMBC were incubated with ODN IMT 504, ODN 2006 as a positive control and ODN IMT 022 as a negative control (FIG. 4). As can be observed, ODN IMT 504 is as active as the CpG ODN 2006 for stimulation of the expression of CD40, MHC I and MHC II on B lymphocytes.
EXAMPLE 8 
Stimulation of Purified B Lymphocytes by Non-CpG Oligonucleotides of This Invention
Human CD19+ (B) cells were purified to more than 95% purity. Table 10 and FIG. 5 shows that the immunostimulatory activity on this purified cells is comparable to the one observed using human PMBC. These results indicate that stimulation by the non-CpG oligonucleotides of this invention on human cells is direct.








TABLE 10







Induction of proliferation, IL6 and IgM secretion on purified B



cells by phosphorothioate non-CpG oliponucleotides of this invention












Proliferation
IL-6
IgM




Index
(pg/ml)
(ng/ml)



(ODN at
(ODN at
(ODN at



0.375 μg/ml)
6.0 μg/ml)
1.5 μg/ml)

















ODN (S)
SEQUENCE (5′–3′)
Ave.
N
SD
Ave.
N
SD
Ave.
N
SD





















IMT 022
TGCTGCAAAAGAGCAAAAGAGCAA
1.28
16
0.80
 1019
5
 665
 469
7
182



 


IMT 021
TGCTGCTTTTGTGCTTTTGTGCTT
24.48
16
5.16
 9588
5
 729
 946
9
386


 


IMT 504
TCATCATTTTGTCATTTTGTCATT
47.95
16
6.41
11002
5
 884
1274
9
252


 


IMT 506
TCTTCTTTTTGTCTTTTTGTCTTT
25.58
16
7.84
10933
3
1628
1083
10
430


 


2006
TCGTCGTTTTGTCGTTTTGTCGTT
60.82
24
9.79
 7631
6
 997
1292
12
245





 


     15





IMT 021 (SEQ. ID No 1);


IMT 022 (SEQ. ID No 8);


IMT 504 (SEQ ID No 2);


IMT 506 (SEQ ID No 4)






EXAMPLE 9 
Immunostimulation by Phosphodiester Non-CpG Oligonucleotides of This Invention
FIG. 6 and Table 11 shows the effect of phosphodiester non-CpG oligonucleotides of this invention on human PMBC. Since phosphodiester oligonucleotides are very sensitive to nucleases they were added to the culture three times (0, 4, and 16 hs) to a final concentration of 30 μg/ml. A very potent phosphodiester CpG ODN (ODN 2080) was used as positive control in cytometric assays (Hartmann G, Weeratna R, Ballas Z K, Payette P, Suparto, I, Rasmussen W L, Wadschmidt M, Sajuthi D, Purcells R H, Davis H L, Krieg A M. Delineation of a CpG phosphorothioate oligodeoxynucleotide for activating primate immune responses in vitro and in vivo. J. Immunol. 164, 1617-1624, (2000)). As can be observed, under these conditions phosphodiester ODNs bearing non CpG motifs of this invention have immunostimulatory activity.








TABLE 11







Immune-stimulation by phosphodiester non-CpG



oligonucleotides of this invention










IL-6 Secretion




(pg/ml)



(ODN at



6.0 μg/ml)











ODN (O)
SEQUENCE (5′–3′)
Avg.
N
SD















IMT 022
TGCTGCAAAAGAGCAAAAGAGCAA
23
8
10



 


IMT 053
TTTTTTTTTTTTTTTTTTTTTTTT
24
4
24


 


IMT 504
TCATCATTTTGTCATTTTGTCATT
207
6
46


 


IMT 506
TCTTCTTTTTGTCTTTTTGTCTTT
403
3
220


 


2006
TCGTCGTTTTGTCGTTTTGTCGTT
228
4
40





IMT 022 (SEQ. ID No 8);


IMT 504 (SEQ ID NO 2);


IMT 053 (SEQ ID No 19);


IMT 506 (SEQ ID No 4)






EXAMPLE 10
Induction of Cell Proliferation in Peripheral White Blood Cells of Monkeys of the Species Cebus Apella and Macaca Fascicularis by Non-CpG ODN(S)s of This Invention
Non-CpG ODN(S)s of this invention are not very effective immunostimulants in mouse, pig and sheep. However, they are effective in monkeys. For example, FIG. 7 shows the immunostimulatory activity of non-CpG ODN(S)s on peripheral white blood cells of monkeys of the species Cebus apella and Macaca fascicularis. According to these results the most effective non-CpG ODNs in primates are those bearing the CATTTTGT motif. Therefore, monkeys can be used as animal models for research on clinical applications of these non-CpG ODN(S)s.
EXAMPLE 11 
Vaccination of Subjects
Vaccine formulations containing the non-CpG ODNs adjuvant of this invention can be used to vaccinate subjects against a variety of bacterial and viral disease agents and against tumoral cells. Table 12 shows the effect of inoculation of monkeys of the species Cebus apella with a vaccine formulation that includes recombinant Hepatitis B surface antigen (rHBsAg) and alumina in presence or absence of ODN IMT 504 or ODN IMT 021 as adjuvant.







TABLE 12







Anti-HBs responses in Cebus apella immunized


against HBsAg with IMT ODNs










Post - prime
Post - boost











ODN (S)
Pre - prime
14 days
28 days
14 days





None
0
0.4 ± 0.1
14 ± 20
190 ± 52 


IMT 021
0
23 ± 32
231 ± 287
902 ± 169


IMT 504
0
10 ± 14
113 ± 114
659 ± 296


2006
0
22 ± 24
67 ± 57
705 ± 315









As can be observed there is a dramatic increment in the title of anti-HBsAg in animals vaccinated with HBsAg plus IMT ODN(S) 504 or 21 as compared with those vaccinated with HBsAg alone. CpG ODN 2006 have a performance as adjuvant similar to the non-CpG ODNs of this invention.
In particular, a human can be vaccinated against hepatitis B by administration of a vaccine formulation that includes recombinant Hepatitis B surface antigen (rHBsAg) and alumina and one or more of the oligonucleotides of this invention as adjuvants. The amount of rHBsAg in each dose, and the administration schedule, can vary as appropriate for the age of the human. For example, for humans from about birth to about 12 years old, a three dose schedule of from about 2.5 μg to about 5 μg of rHBsAg can be administered at 0, 1-3 months afterward and 4-18 months afterward, preferably at 0, 2 and 6 months. One or more of the oligonucleotides of this invention can be present in the formulation from about 10 μg to about 1,000 μg per dose.
For humans from about 12 to about 60 years old, a three dose schedule of from about 5 μg to about 40 μg of rHBsAg can be administered at 0, 1-3 months afterward and 4-18 months afterward, preferably at 0, 2 and 6 months. One or more of the oligonucleotides of this invention can be present in the formulation from about 10 μg to about 1,000 μg per dose.
EXAMPLE 12 
Stimulation of the Expression of Costimulatory Molecules in Malignant B-Lymphocytes  by Phosphorothioate Non-CpG Oligonucleotides
The stimulation of malignant B lymphocytes recovered from blood of patients suffering chronic lymphocytic leukemia (CLL) by phosphorothioate non-CpG oligonucleotides of this invention was examined by flow cytometric (FACS) analysis. The results of a typical FACS analysis are shown in FIG. 8.
As can be seen the phosphorothioate non-CpG oligonucleotides IMT 021 and IMT 504 of this invention are able to stimulate expression of CD86, CD40 and MHC class I surface costimulatory molecules on malignant B lymphocytes as well as the CpG ODN 2006.
EXAMPLE 13 
Stimulation of the Expression of Costimulatory Molecules on Plasmacytoid Dendritic Cells by Phosphorothioate Non-CpG Oligonucleotides of This Invention
The stimulation of plasmacytoid dendritic cells recovered from blood of normal donors by phosphorothioate non-CpG oligonucleotides of this invention was examined by flow cytometric (FACS) analysis. The results of a typical FACS analysis are shown in FIG. 9. As can be seen the phosphorothioate non-CpG oligonucleotides IMT 021 and IMT 504 of this invention are able to stimulate expression of CD86, CD40 and MHC class I surface molecules on plasmacytoid dendritic cells as well as the CpG ODN 2006.
EXAMPLE 14 
Treatment of Subjects With Tumoral Disease
Pharmaceutical formulations containing one or more of the oligonucleotides immunostimulatory of this invention, can be used to treat subjects against a variety of tumoral diseases. In particular, a human with a Melanoma can be treated by administration of a pharmaceutical formulation containing the oligonucleotide of this invention as the active component. The amount of oligonucleotide in each dose, and the administration-schedule, can vary appropriate for the corporal mass of the subject and stage in tumor progression. For example, for a human of about 70 Kg. having an advance, unresectable metastatic melanoma, a dose of about 1 mg of the oligonucleotide of this invention can be administered 3 times per week during about 10 weeks.

## Claims

1. An immunostimulatory oligonucleotide having 24 to 100 nucleotides, comprising the nucleotide sequence










TCTTCTTTTTGTCTTTTTGTCTTT.
(SEQ ID No 4)

2. The immunostimulatory oligonucleotide of claim 1, wherein the immunostimulatory oligonucleotide is encapsulated in a slow release delivery vehicle.

3. The immunostimulatory oligonucleotide of claim 1, wherein the immunostimulatory oligonucleotide is included in a pharmaceutically acceptable carrier.

4. A pharmaceutical composition comprising an immunostimulatory oligonucleotide according to claim 1 and a pharmaceutically acceptable carrier.

5. A composition according to claim 4, wherein said immunostimulatory oligonucleotide is included in a plasmid.

6. A composition according to claim 4, further comprising an antigen.

7. A composition according to claim 6, wherein the antigen is selected from the group consisting of viruses, bacteria, fungi, parasites, tumor cells, toxins, allergens, proteins, glycolipids, and polysaccharides.

8. A composition according to claim 6, wherein the antigen is a viral antigen, a bacterial antigen, a human or animal tumor cell antigen, or a fungal antigen.

9. A composition according to claim 4, further comprising a plasmid encoding an antigen.

10. A composition according to claim 9, wherein the encoded antigen is a viral antigen, a bacterial antigen, a human or animal tumor cell antigen, or a fungal antigen.

11. An immunostimulatory oligonucleotide consisting of the nucleotide sequence










TCTTCTTTTTGTCTTTTTGTCTTT.
(SEQ ID No 4)