# Patent

## Title
Macroporous material in the form of plastic pearls

## Abstract

The invention relates to a macroporous plastics bead material with an average particle diameter from 10 to 1000 μm, containing a copolymer of a) 5-60 wt. % vinylically polymerizable monomers with a water solubility of at least 1% at 20° C., b) 1-40 wt. % vinylically polymerizable monomers with an additional functional group, which can enter into covalent bonds with the nucleophilic groups of the ligands in a polymer-like reaction, c) 10-40 wt. % hydrophilic, crosslinking radical-polymerizable monomers with two or more ethylenically unsaturated polymerizable groups and d) 10-60 wt. % vinylically polymerizable monomers with a water solubility of at most 1% at 20° C. are contained, as a rule with the monomers a) to d) adding up to 100%.

## Background
The invention relates to the field of polymeric support systems and in particular a macroporous plastics bead material containing a copolymer of crosslinked (meth)acrylate plastic.
PRIOR ART
Polymeric support systems containing copolymers of crosslinked (meth)acrylate plastic are known, e.g. from EP-A 0 328767, EP-A 424 130, EP-A 579 928 or from WO 99/33964.
WO 99/40122 (DE-A 19804518) relates to a method of production of a bead-like, crosslinked hydrophilic copolymer with binding activity with respect to ligands with nucleophilic groups, by inverse suspension polymerization of a monomeric phase. The invention further relates to the polymeric support materials obtainable therefrom, with high binding capacity for penicillin amidase and a low swelling index, and their uses.
The copolymer of crosslinked (meth)acrylate plastic is obtained by inverse suspension polymerization of the following monomers:

    a) 5-40 wt. % hydrophilic radical-polymerizable monomers with a vinyl group, which form at least 10% aqueous solutions at room temperature
    b) 30-50 wt. % radical-polymerizable monomers with a vinyl group and an additional functional group which, in a polymer-like reaction, can enter into covalent bonds with the nucleophilic groups of the ligands
    c) 20-60 wt. % hydrophilic, crosslinking radical-polymerizable monomers with two or more ethylenically unsaturated polymerizable groups


The copolymer is suitable as support material for various types of enzymes.
OBJECT AND SOLUTION
On the basis of WO 99/40122, other macroporous plastics bead materials were to be prepared, which are suitable as efficient support materials especially for enzymes that are able to convert relatively hydrophobic substrates, preferably for lipases. If possible, the advantageous properties of the known materials according to WO 99/40122, e.g. their low swelling indices, were not to be impaired. Furthermore, macroporous plastics bead materials were to be prepared which, after binding of lipases, display comparatively high activities in test systems such as tributyrin hydrolysis, triacetin hydrolysis, phenyl ethyl acetate hydrolysis and/or phenyl ethyl acetate synthesis.
The object is achieved by means of a macroporous plastics bead material with an average particle diameter in the range from 10 to 1000 μm, containing a copolymer of

    
    
        a) 5-60 wt. % vinylically polymerizable monomers with a water solubility of at least 1% at 20° C.,
        b) 1-40 wt. % vinylically polymerizable monomers with an additional functional group, which can enter into covalent bonds in a reaction with nucleophilic groups of ligands,
        c) 10-40 wt. % hydrophilic, crosslinking radical-polymerizable monomers with two or more ethylenically unsaturated polymerizable groups, characterized in that, additionally,
        d) 10-60 wt. % vinylically polymerizable monomers with a water solubility of at most 1% at 20° C. are contained, with the monomers a) to d) as a rule adding up to 100%.
    
    


IMPLEMENTATION OF THE INVENTION
Average Particle Diameter V50 
The macroporous plastics bead material has an average particle daimeter V50 from 10 to 1000, preferably from 50 to 600, particularly preferably from 100 to 500, and especially frim 200 to 400 μm.
The average particle diameter V50 can be determined for example with a particle analyzer. For this, as a rule a little detergent is added as wetting agent to a sample with a particle concentration in the range 103-106, additionally treating with ultrasound prior to measurement in order to seperate the particles.
Porosity
The macroporous plastics bead material can have a porosity that can be characterized by a KPS10 value from 0.3 to 0.9, especially from 0.35 to 0.6, which can be measured e.g. by means of reverse size-exclusion chromatography in tetrahydrofuran.
Swelling Index in Water
The macroporous plastics bead material can have a swelling index in water from 1 to 1.5, especially from 1.05 to 1.2.
Monomer Composition
The copolymer consists according to the invention of the radical-polymerized units of monomer types a) to d), which are in the stated quantitative proportions and as a rule add up to 100 wt. %. The ability to convert hydrophobic substrates is determined essentially by the actual choice of monomers and their quantitative proportions. In particular the balance of the more hydrophilic monomer types a) and the more hydrophobic monomer types d) seems to have an important influence on the conversion of hydrophobic substrates.
The enzyme binding property is influenced in particular by the choice and the proportion of monomer type b). Monomer type c) influences, as crosslinking agent, the size and porosity of the plastics beads. The size and porosity, in their turn, influence the binding capacity for enzymes and their catalytic behavior in the bound state.
It will be evident to a person skilled in the art that the copolymers can also contain small proportions of other vinylically polymerizable monomers, without this necessarily impairing their essential properties in an individual case. As a rule, however, the copolymers consist to 100% of radical-polymerized units the monomers a) to d).
Monomers a)
5-60, preferably 10 to 50 wt. % vinylically polymerizable monomers with a water solubility of at least 1% at 20° C. are contained as monomers a) in the copolymer.
The following are suitable in particular as monomers a): acrylamide and/or methacrylamide, hydroxyalkyl esters of unsaturated polymerizable carboxylic acids, such as hydroxyethyl acrylate, hydroxyethyl methacrylate, hydroxypropyl acrylate, and hydroxypropyl methacrylate. Also suitable are for example N-vinyl pyrrolidone, methyl methacrylate or 3-allyloxy-1,2-propanediol. Methyl methacrylate and methoxypolyethyleneglycol methacrylate (MPEGMA), polyethyleneglycol methacrylate (PEGMA) are preferred, especially methoxypolyethyleneglycol methacrylate 350 (MPEGMA350) and polyethyleneglycol methacrylate (PEGMA).
Good results are obtained in particular when the copolymer contains methoxypolyethyleneglycol methacrylate, e.g. MPEGMA350, and methyl methacrylate simultaneously. In total, it can contain e.g. 30 to 45 wt. % of the two monomers.
Monomers b)
1-40, preferably 10 to 30 wt. % vinylically polymerizable monomers, which are provided with an additional functional group, preferably an oxirane group (epoxy group), which can enter into covalent bonds in a reaction with nucleophilic groups of ligands, are contained as monomers b).
The term ligands preferably means biologically active molecules, especially macromolecules, e.g. amino acids, peptides, proteins, especially enzymes such as lipases, but also nucleic acids or polysaccharides. In particular oxirane groups are suitable, for binding ligands while preserving their biological activity.
Suitable monomers b) are glycidyl methacrylate and/or allylglycidyl ether and/or vinylazlactone.
Glycidyl methacrylate is preferred, and is especially preferred in an amount from 15 to 25 wt. %.
Monomers c)
10-40, preferably 20 to 35 wt. % of hydrophilic, crosslinking, radical-polymerizable monomers with two or more ethylenically unsaturated polymerizable groups are contained as monomers c).
Those that are suitable are e.g. hydrophilic di(meth)acrylates, such as ethyleneglycol-di(meth)acrylate and polyethylene-oxide-di(meth)acrylates. Other suitable monomers c) are N,N′-methylene-bis-acrylamide or N,N′-methylene-bis-methacrylamide.
The preferred monomer c) is 1,4-butanediol dimethacrylate.
Monomers d)
10-60, preferably 10 to 30 wt. % vinylically polymerizable monomers with a water solubility of at most 1% at 20° C. are contained as monomers d).
Suitable monomers d) are e.g. isobutyl methacrylate, n-butyl methacrylate, cyclohexyl methacrylate, benzyl methacrylate and 2-ethylhexyl methacrylate.
Method of Production
The macroporous plastics bead material consisting of the copolymers can be produced in a known way by suspension polymerization (bead polymerization). A stirred reactor is charged with an aqueous phase, which in particular contains distributors, e.g. precipitated aluminum hydroxide. The monomers are then added together with a polymerization initiator, e.g. dilauryl peroxide, in an organic phase, e.g. cyclohexane. The phases are dispersed with stirring, and polymerization of the monomers can take place e.g. in a temperature range from 60 to 80° C. The macroporous plastics bead material can be obtained from the charge by filtration and drying.
Variants of the Copolymers/Test Systems
Variants of the copolymers on which the enzyme lipase from Candida antarctica was immobilized should show good results in the following test systems. The enzyme can be immobilized e.g. by incubation of the macroporous plastics bead material with a commercial enzyme solution (e.g. Novozym® 525F) in aqueous solution for 1 to 2 days at room temperature. Then the plastics bead material coupled to the enzyme is washed and dried. The immobilizates thus obtained can be tested in the following test systems.
Hydrolysis of tributyrin (aqueous system and water-soluble substrate). The immobilizate of macroporous plastics bead material and lipase from Candida antarctica can for example have a tributyrin-hydrolysis activity of at least 150 [U/g], preferably at least 300 [U/g], especially of at least 500 [U/g].
Hydrolysis of triacetin (aqueous system, water-insoluble substrate). The immobilizate of macroporous plastics bead material and lipase from Candida antarctica can for example have a triacetin-hydrolysis activity of at least 50 [U/g], preferably at least 100 [U/g], especially of at least 150 [U/g].
Hydrolytic racemate cleavage of 1-phenylethyl acetate (aqueous system, water-insoluble substrate). The immobilizate of macroporous plastics bead material and lipase from Candida antarctica can for example have a phenylethyl acetate-hydrolysis activity of at least 80 [U/g], preferably at least 100 [U/g], and especially of at least 150 [U/g].
Racemate cleavage of 1-phenylethanol by transesterification (nonaqueous system). The immobilizate of copolymer and lipase from Candida antarctica can for example have a phenylethanol-racemate cleavage activity of at least 100 [U/g], preferably at least 120 [U/g], and especially of at least 180 [U/g].
Uses
The macroporous plastics bead material can be used for immobilizing peptides, proteins, nucleic acids or polysaccharides, preferably for immobilizing lipases from Rhizopus, Aspergillus, Mucor, Alcaligenes, Candida, Pseudomonas, Thermomyces, Chromobacterium, pig pancreas, and for immobilizing phospholipases from Streptomyces and Actinomadura, as well as for immobilizing esterases from pig's liver and orange-peel.
The macroporous plastics bead materials according to the invention can be used as support materials for covalent bonding of ligands by means of the oxirane groups that are present, in stirred or flow reactors. This can take place e.g. by deposition of proteins, especially enzymes, from concentrated solutions via covalent bonding, preserving their biological activity. Furthermore, it is also possible for peptides, amino acids, lipids, nucleotides, polynucleotides, low-molecular nucleophilic compounds or organometallic compounds to be converted with the oxirane groups of the support beads.
The polymer beads laden with ligands can be used in a known way for stereospecific synthesis of chiral substances, such as amino acids (d-phenylalamine, p-hydroxy-d-phenylalanine, 1-tert-leucine) or pharmaceuticals, e.g. β-lactam antibiotics, ibuprofen.
Another area of application includes syntheses of fine chemicals or basic products for chemical syntheses (e.g. malic acid). The polymer beads can also be used in separation technology for adsorption chromatography or gel-permeation chromatography. For specific adsorption, the polymer beads can be loaded with immunoglobulin fractions from antisera or with monoclonal antibodies.
Another field of application is the use of the polymeric support material laden with enzymes or antibodies as an adsorbent in extracorporeal therapy, in which pathogenic or toxic substances are removed from whole blood.


EXAMPLES
1. Examples of Copolymer Variants










Synoptic table of the copolymer variants











Copolymer variants No. + Proportion


Monomer

of monomer, wt. %


















type
Monomer
13
60
66
61
67
15
27
30
37
39





















a)
Methyl methacrylate
27.5
20.0
17.5
15.0
12.5
22.5
27.5
22.5
25.0
25.0



MPEGMA350

15.0
20.0
25.0
30.0



3-Allyloxy-1,2-





10



propanediol



Hydroxyethyl methacrylate







10


b)
Glycidyl methacrylate
20.0
20.0
20.0
20.0
20.0
20.0
20.0
20.0
20.0
20.0


c)
1,4-Butanediol
25.0
25.0
25.0
25.0
25.0
25.0
25.0
25.0
30.0
30.0



dimethacrylate


d)
Isobutyl methacrylate
27.5
20.0
17.5
15.0
12.5
22.5

22.5



Cyclohexyl methacrylate








25.0



Benzyl methacrylate






27.5



2-Ethylhexyl methacrylate









25.0





MPEGMA350 = Methoxypolyethyleneglycol methacrylate 350






Example 1
Production Instructions for Copolymer Variant 13
Proportions of the Phases:


    Water:monomer (+solvent)=2:1 (can be varied), e.g. 1:1 or 1:2.
    Monomers:solvent=2:1 (can be varied), e.g. 1:1 or 1:2


The whole amount of water [810 g] and the aluminum sulfate [5.4 g] are placed in a 2 liter stirred reactor, comprising thermocouple, bath thermostat, reflux condenser, and nitrogen inlet pipe, with stirring and nitrogen feed, and heated to 70° C. When the specified internal temperature is reached, the soda solution [24 g] is added in one go for precipitating the aluminum hydroxide. Then the auxiliary distributors are added: C15-paraffin sulfonate, sodium salt and polyethyleneglycol 5000/6000 [0.05 g each]. The pH value of the aqueous phase is approx. 5.5.
Then


    
    
        74 g isobutyl methacrylate,
        74 g methyl methacrylate,
        54 g glycidyl methacrylate,
        67.5 g 1,4-butanediol dimethacrylate and
        5.4 g dilauroyl peroxide,

dissolved in 135 g cyclohexane, are added. In the next 20 minutes the internal temperature rises from 70° C. to 72° C. After the temperature maximum, heating continues at 70° C. for a further 2 hours. Then it is cooled to 40° C. and acidified with 10 ml of 50% sulfuric acid. The charge is cooled further, drained on a porcelain suction filter (cloth filter) and washed with 5 L deionized H2O. The moist beads are dried in a fluidized-bed dryer at 50-60° C. for 90 minutes.

    
    


Example 2
Production Instructions for Copolymer Variant 60
The whole amount of water [810 g] and the aluminum sulfate [5.4 g] are placed in a 2 liter stirred reactor, comprising thermocouple, bath thermostat, reflux condenser, and nitrogen inlet pipe, with stirring and nitrogen feed, and heated to 70° C. When the specified internal temperature is reached, the soda solution [24 g] is added in one go for precipitating the aluminum hydroxide. Then the auxiliary distributors are added: C15-paraffin sulfonate, sodium salt and polyethyleneglycol 5000/6000 [0.05 g each]. The pH value of the aqueous phase is approx. 5.5.
Then


    
    
        54 g isobutyl methacrylate,
        54 g methyl methacrylate,
        54 g glycidyl methacrylate,
        67.5 g 1,4-butanediol dimethacrylate
        40.5 g methoxypolyethyleneglycol methacrylate and
        5.4 g dilauroyl peroxide,

dissolved in 135 g cyclohexane, are added. In the next 20 minutes the internal temperature rises from 70° C. to 72° C. After the temperature maximum, heating continues at 70° C. for a further 2 hours. Then it is cooled to 40° C. and acidified with 10 ml of 50% sulfuric acid. The charge is cooled further, drained on a porcelain suction filter (cloth filter) and washed with 5 L deionized H2O. The moist beads are dried in a fluidized-bed dryer at 50-60° C. for 90 minutes.

    
    


Example 3
Polymer Analysis of the Copolymer Variants
















Variant
V50
KPS10
Swelling



No.
[μm]
value
index





















13
356
0.46
1



60
227
0.51
1



66
243
0.51
1



61
294
0.52
1.1



67
269
0.53
1.1



15
340
0.52
1.1



27
384
0.51
1



30
316
0.56
1.1



37
392
0.55
1



39
388
0.4
1










Determination of the Average Particle Diameter V50:

The average particle diameter is measured with a CIS1 particle analyzer from the company L.O.T. GmbH. Sample preparation: a sufficient amount of the sample is placed in a 400 ml glass beaker to give a concentration of 103-106 particles. Then a few drops of a detergent are added to the sample to aid wetting. Then it is topped up with 350 ml of fully deionized water. The suspension obtained is treated with ultrasound for approx. 1 minute and then measurements are taken in the CIS1 instrument, measurement range 5-600 μm.
Determination of the Distribution Coefficient KPS10:
Using reverse size-exclusion chromatography (SEC), the proportion of the pore volume accessible to the probe molecule employed is determined. The distribution coefficient thus obtained is a measure of the porosity of the sample. A Merck Superformance glass column 300×10 mm is filled according to the balanced density principle with THF and tetrachloroethylene with the bead polymer. Then the column is packed and equilibrated, by supplying 150 ml THF at a flow rate of 15 ml/min. Then the column is sealed and a further 150 ml tetrahydrofuran (THF) is supplied at a flow rate of 10 ml/min. Pressures>20 bar are to be avoided, as the column is then compressed too much and suitable column packing cannot be achieved. Then the probe molecules are supplied to the column successively and eluted at a flow rate of 0.2 ml/min. The exclusion limits are determined with o-dichlorobenzene and polystyrene 6770000 (molecular weight 6770000 dalton), with polystyrene 10200 (molecular weight 10200 dalton) as probe molecule.
The distribution coefficient is calculated from the elution volumes as follows:

KPS10=VE(PS10200)−VE(PS6770000)/VE(o-dichlorobenzene)−VE(PS6770000)

where:

    KPS10=distribution coefficient for polystyrene 10200,
    VE(PS10200)=elution volume of the probe molecule polystyrene 10200,
    VE(PS6770000)=elution volume of the exclusion marker polystyrene 6770000,
    VE(o-dichlorobenzene)=elution volume of the exclusion marker o-dichlorobenzene.

Determination of the Swelling Index:



3 g of support are weighed in a 25 ml graduated cylinder with 0.5 ml divisions. The surface is leveled by light tamping of the graduated cylinder and the filled volume VT in ml is noted. Next, 9 ml of 0.01% Polysorbate 80 solution in ultrafiltered water is added and the graduated cylinder is sealed. Within the space of one hour, the graduated cylinder is shaken vigorously four times, at 15 minute intervals. Then beads adhering to the wall are rinsed down with 2 to 3 ml of 0.01% Polysorbate 80 solution in ultrafiltered water. The graduated cylinder is left still for 2 hours, then the volume of the settled and of the floating material is read and added to VQ. The swelling index is defined as the ratio VQ/VT.
Example 4
Immobilization Methods
Method M2:
Add 4 ml of enzyme solution Novozym 525F to 16 ml of a 10 vol. % solution of tert-amyl alcohol in distilled water. Add this solution to 2 g of dry support. Swirl the preparation at 20° C. for 1.5 d. Then rinse the beads onto a Por 2 glass frit and wash with 6×50 ml distilled water. After the last rinse, filter with strong suction. Dry over xerogel in a desiccator.
Method M3:
Mix 4 ml of enzyme solution Novozym 525F and 6 ml of ultrapure water. Add the mixture to 2 g of dry support. Swirl, then shake while inverted at room temperature for 1.5 d. Then rinse the beads onto a Por 2 glass frit and wash with 3×50 ml distilled water and 3×50 ml 0.1M potassium phosphate buffer pH 7.5. After the last washing, filter with strong suction and dry over xerogel in a desiccator.
Method M3-aMEK:
Mix 4 ml of enzyme solution Novozym 525F and 6 ml of ultrapure water. Add the mixture to 2 g of dry support. Swirl, then shake while inverted at room temperature for 1.5 d. Then rinse the beads onto a Por 2 glass frit and wash with 6×50 ml distilled water. For drying, filter with strong suction and wash chromatographically with 6×50 ml methyl ethyl ketone (MEK) on the glass frit, then filter with suction until dry (approx. 30 min). Instead of MEK it is possible to use other, partially or completely water-miscible solvents such as THF, tert-amyl alcohol, isopropanol, and acetone.
Method M4:
Mix 4 ml of enzyme solution Novozym 525F and 6 ml of 1M potassium phosphate buffer pH 7.5. Add the mixture to 2 g of dry support. Swirl, then leave to stand at 23° C. for 1.5 d. Then rinse the beads onto a Por 2 glass frit and wash with 3×50 ml distilled water and 3×50 ml 0.1M potassium phosphate buffer pH 7.5. After the last rinse, filter with strong suction. Dry over xerogel in a desiccator.
Example 5
Activity Tests
5.1 Tributyrin Hydrolysis
In the activity test, the release of butyric acid is measured by titration with 1M NaOH solution in conditions of constant pH.
Equipment:


    pH-stat Titrator, e.g. Mettler DL 50
    100 ml glass vessel (similar to the standard vessel from Mettler-Toledo)
    pH electrode DG-111-SC
    10 ml burette
    Mettler propeller stirrer, speed 80%

Preparation Size:

    48.5 ml buffer pH 7.0 Fluka 82571
    5 mmol tributyrin (100 mM)
    50 mg enzyme immobilizate

Procedure:



1.47 ml (5 mmol) of tributyrin were emulsified in 48.5 ml of 0.5 M phosphate buffer pH 7.0 using a propeller stirrer for 5 min at room temperature. The pH value of the emulsion was adjusted to pH 7.0 by adding 1M sodium hydroxide solution (pre-titration). Then 50 mg of immobilizate is added, starting the reaction. The connected titrator records the consumption of sodium hydroxide solution as a function of the hydrolysis time. The activity of the enzyme is determined from the linear slope of the curve. The activity in U/g immobilizate can be calculated directly from the value obtained from that in ml/min per amount of enzyme used.
5.2 Triacetin Hydrolysis
In the activity test, the release of acetic acid is measured by titration with 0.1 M NaOH solution in conditions of constant pH.
Equipment:


    pH-stat Titrator, e.g. Schott Titroline Alpha
    100 ml double-jacketed glass vessel
    Water bath with thermostatic control
    Radiometer TTA 80 stirrer stand
    pH electrode Schott Blue Line
    10 ml burette

Preparation Size:

    16 ml 0.05M potassium phosphate buffer pH 7.0
    400 μl Triacetin Merck No. 108238
    100 mg enzyme immobilizate

Procedure:



The reaction vessel is thermostated to 25° C. It is charged with 16 ml of 0.05M potassium phosphate buffer pH 7.0, and 100 mg of the immobilizate to be investigated is added. Then stirring is started. Reaction is initiated by adding 400 μl triacetin. The connected titrator records the consumption of sodium hydroxide solution as a function of the hydrolysis time. The activity of the enzyme is determined from the linear slope of the curve. The activity in U/g immobilizate can be calculated directly from the value obtained from that in ml NaOH/min per amount of enzyme used.
5.3 Phenyl Ethyl Acetate Hydrolysis
In the activity test, the release of acetic acid from (±)-1-phenyl ethyl acetate is measured by titration with 1M NaOH solution in conditions of constant pH.
Equipment:


    pH-stat Titrator, e.g. Mettler DL 50
    25 ml semimicro vessel made of glass (standard vessel from Mettler-Toledo)
    Micro-pH electrode Mettler N 6000 A
    5 ml burette
    Mettler micro-propeller stirrer, speed 100%

Preparation Size:

    19.2 ml buffer pH 7.0 Fluka 82571
    5 mmol (±)-1-phenyl ethyl acetate (250 mM)
    100 mg enzyme immobilizate

Procedure:



800 μl (5 mmol) of (±)-1-phenyl ethyl acetate was emulsified for 5 min at room temperature in 19.2 ml of 0.5 M phosphate buffer pH 7.0 using a micro-propeller stirrer. The pH value of the emulsion was adjusted to pH 7.0 by adding 1 M sodium hydroxide solution (pre-titration). Then 100 mg of immobilizate is added, starting the reaction. The connected titrator records the consumption of sodium hydroxide solution as a function of the hydrolysis time. The activity of the enzyme is determined from the linear slope of the curve. The activity in U/g immobilizate can be calculated directly from the value obtained from that in ml/min per amount of enzyme used.
After approx. 50% conversion of the acetate, the reaction is stopped, the enzyme is filtered off and then washed with methyl-tert-butyl ether (MTBE). The hydrolyzate is extracted with MTBE three times and the organic phases are combined and dried over MgSO4 and the solvent is concentrated by a rotary evaporator. The conversion and the enantiomer excesses for educt and product are determined in the residue by HPLC on a chiral column (Daicel Chiralcel OD-H).
HPLC Method:


    
    
        Coating: hexane: t-butanol 98.0:2.0
        Flow 0.5 ml/min>1.0 ml/min
        Detection 254 nm
        Column furnace temp. 20° C.
        (±)-1-Phenyl ethyl acetate (R) or (S)Rt=11.1 min; Rt=11.9 min
        Phenylacetone Rt=14.9 min
        (±)-1-Phenylethanol (R) or (S)Rt=25.6 min; Rt=39.1 min

5.4 Phenyl Ethyl Acetate Synthesis

    
    


The esterification of (±)-1-phenylethanol with vinyl acetate to (R)-1-phenyl ethyl acetate is determined. The progress of the reaction is determined by HPLC, in which the synthesis of (R)-1-phenyl ethyl acetate and the decrease in (R)-1-phenylethanol are observed. The specific activity of the enzyme is then calculated from the slope of the synthesis curve.
Equipment:


    25 ml and 10 ml screw-cap glass vessels
    Ika orbital shaker (500 rpm)

Preparation Size:

    20 ml and 8 ml MTBE
    2 mmol and 0.8 mmol (±)-1-phenylethanol
    6 mmol and 2.4 mmol vinyl acetate
    50 mg enzyme immobilizate

Procedure:



0.8 mmol (±)-1-phenylethanol and 2.4 mmol vinyl acetate were shaken on an orbital shaker at 500 rpm in 8 ml MTBE in 10 ml screw-cap glass vessels at 40° C. reaction temperature. In defined time intervals of 10 min, 20 μl samples were taken up to the first hour, 400 μl acetonitrile was added and analysis was carried out on an HPLC column. The conversion for determination of enzyme activity is determined on an RP-18-HPLC column (column Lichrosphere RP-18 (250 mm×5 μm), water:acetonitrile 50:50, flow 0.5 ml; 254 nm; room temperature). The enantiomer excess is determined by HPLC on a chiral phase (Daicel Chiralcel OD-H). For HPLC method, see above.
Example 6
Activity of the Immobilizates







TABLE A







Examples of polymer supports made according to


the invention, immobilization method M3-aMEK.












Copol-
Immo-
Tributyrin
Triacetin
PEAc-Hyd.
PEAc-Syn.


ymer
bilization
activity
activity
activity
activity


No.
method
[U/g]
[U/g]
[U/g]
[U/g]





13
M3-aMEK
463
 96
106
184


60
M3-aMEK
861
172
183
184


66
M3-aMEK
793
192
101
159


61
M3-aMEK
457
114
 82
189


67
M3-aMEK
188
 55
 19
198









For the two columns on the right: enantiomeric purity of the products in all cases >99% ee.







TABLE B







Examples of polymer supports made according to


the invention, immobilization method M2.












Copol-
Immo-
Tributyrin
Triacetin
PEAc-Hyd.
PEAc-Syn.


ymer
bilization
activity
activity
activity
activity


No.
method
[U/g]
[U/g]
[U/g]
[U/g]















13
M2
345
n.d.
136
85


15
M2
369
n.d.
114
97


27
M2
282
n.d.
66
72


30
M2
281
n.d.
73
82


37
M2
401
n.d.
93
86


39
M2
270
n.d.
126
110





n.d. = not determined






For the two columns on the right: enantiomeric purity of the products in all cases >99% ee.







TABLE C







Comparative examples (immobilization methods


M3 and M4).













Immo-
Tributyrin
Triacetin
PEAc-Hyd.
PEAc-Syn.



bilization
activity
activity
activity
activity


Polymer
method
[U/g]
[U/g]
[U/g]
[U/g]















A
M3
23

19
1


A
M4
48
85
6
1


B
M3
55
40
54
59


B
M4
114
203
171
22


C
M3
70

44
6


C
M4

155









For the two columns on the right: enantiomeric purity of the products in all cases >99% ee.
Polymer A: Commercially available macroporous crosslinked plastics bead material consisting of

    
    
        10 wt. % methacrylamide
        20 wt. % glycidyl methacrylate
        20 wt. % allyl glycidyl ether
        50 wt. % N,N′-methylene-bis-methacrylamide,
    
    


Polymer B: Commercially available macroporous crosslinked plastics bead material consisting of

    
    
        30 wt. % methacrylamide
        20 wt. % glycidyl methacrylate
        20 wt. % allyl glycidyl ether
        30 wt. % N,N′-methylene-bis-methacrylamide,
    
    


Polymer C corresponds to polymer A in the monomer composition, but has a lower swelling index (<1.5) (production according to DE-A 19804518).

## Claims

1. A macroporous plastics bead material with an average particle diameter from 10 to 1000 μm, comprising a copolymer of:
a) 5-60 wt. % vinylically polymerizable monomers with a water solubility of at least 1% at 20° C. selected from the group consisting of acrylamide, methacrylamide, hydroxyalkyl esters of unsaturated polymerizable carboxylic acids, N-vinylpyrrolidone, methyl methacrylate, 3-allyloxy-1,2-propanediol, methoxypolyethyleneglycol methacrylate and polyethyleneglycol methacrylate,
b) 1-40 wt. % vinylically polymerizable monomers with an additional functional group, which can enter into covalent bonds in a reaction with nucleophilic groups of ligands,
c) 10-40 wt. % hydrophilic, crosslinking radical-polymerizable monomers with two or more ethylenically unsaturated polymerizable groups, and
d) 10-60 wt .% vinylically polymerizable monomers with a water solubility of at most 1% at 20° C. selected from the group consisting of isobutyl methacrylate, n-butyl methacrylate, cyclohexyl methacrylate, benzyl methacrylate and 2-ethylhexyl methacrylate, wherein the wt. % of monomers a) to d) add up to 100%.

2. The macroporous plastics bead material as claimed in claim 1, wherein the macroporous plastics bead material has a KPS10 porosity value of said macroporous plastics bead material is 0.3 to 0.9 as measured by reverse size-exclusion chromatography in tetrahydrofuran.

3. The macroporous plastics bead material as claimed in claim 1, wherein the macroporous plastics bead material has a swelling index in water of 1 to 1.5.

4. The macroporous plastics bead material as claimed in claim 1, wherein said vinylically polymerizable monomer (a) is selected from the group consisting of methoxypolyethyleneglycol methacrylate and methyl methacrylate.

5. The macroporous plastics bead material as claimed in claim 1, further comprising immobilized lipase from Candida antarctica having a tributyrin-hydrolysis activity of at least 150 [U/g].

6. The macroporous plastics bead material as claimed in claim 1, further comprising immobilized lipase from Candida antarctica having a phenyl ethyl acetate-hydrolysis activity of at least 80 [U/g].

7. The macroporous plastics bead material as claimed in claim 1, further comprising immobilized lipase from Candida antarctica having a phenylethanol-racemate cleavage activity of at least 100 [U/g].

8. A method for production of the macroporous plastics bead material as claimed in claim 1 comprising providing a monomer combination of:
a) 5-60 wt. % vinylically polymerizable monomers with a water solubility of at least 1% at 20° C. selected from the group consisting of acrylamide, methacrylamide, hydroxyalkyl esters of unsaturated polymerizable carboxylic acids, N-vinylpyrrolidone, methyl methacrylate, 3-allyloxy-1,2-propanediol, methoxypolyethyleneglycol methacrylate and polyethyleneglycol methacrylate,
b) 1-40 wt. % vinylically polymerizable monomers with an additional functional group, which can enter into covalent bonds in a reaction with nucleophilic groups of ligands,
c) 10-40 wt. % hydrophilic, crosslinking radical-polymerizable monomers with two or more ethylenically unsaturated polymerizable groups, and
d) 10-60 wt. % vinylically polymerizable monomers with a water solubility of at most 1% at 20° C. selected from the group consisting of isobutyl methacrylate, n-butyl methacrylate, cyclohexyl methacrylate, benzyl methacrylate and 2-ethylhexyl methacrylate, wherein the wt. % of monomers a) to d) add up to 100%, and
suspension polymerizing said monomer combination in the presence of an initiator to obtain said macroporous plastics bead material.

9. A method for immobilizing ligands, comprising:
covalently bonding ligands to said macroporous plastics bead material as claimed in claim 1.

10. The method of claim 9, wherein said ligand is a lipase.

11. The method of claim 9, wherein said ligands are selected from the group consisting of amino acids, peptides, proteins, enzymes, antibodies, nucleic acids, polysaccharides, and mixtures thereof.