February 5, 2009 

~f C1 

r . ~= I~~n 

Ref: TS-C083FN 

uOt-Vany Sanitized 

VIA COURIER 

Attn . : US Environmental Protection Agency 

OPPT 

CBIC 

EPA East Bldg . Room 6428 

1201 Constitution Ave. 

Washington, DC 20004-3302 

202-564-8930 or 202-564-8940 . 

Dear Sir: 

Phone[ J 

Fax [ ] 

[ ] is submitting a PMN for 1 chemical substance, TS-C083FN . The 

appropriate fee has been paid via pay.gov marked with the same identifying numbers . 

If you have any questions, please call me at the numbers listed above 

Sincerely, 

l 

Enclosure 
p. 2 

U.S . ENVIRONMENTAL PROTECTION AGENCY 

:.FPA PREMANUFACTURE 

NOTICE 

ES 

CnLGI tile LUIAI IIUUwIe vl pu=Io 

in the Premanufacture Notice 

. 

GENERAL INSTRUCTIONS 

Form Approved . O.M .B . No. 2070-(I(112. Approval Expires 10-31-96 

koe of "-j" 

TS - $ 3 

You must provide all information requested in this form to the extent that it is known to or reasonabl_v ascertainable bv you Make reasonable estimates If you do 

not have actual data 

Before you complete this form . you should read the "Instructions Manual for Premanufacture Notification' (the Instructions Manual is available from tile Toxic 

Substances Control Act (TSCA) Information Service bv calling 202-554-1404 . or taxing 20=-554-5603) . 

If a user fee has been remitted for this notice (40 CFR 700.45) . indicate m the boxes above the TS-user fee identification number you have generated Remember. 

your user fee ID number must also appear on your corresponding fee remittance- which is sent to EPA. HQ Accounune Operations Branch (PM-226) . P O. 

360399M, Pittsburgh, PA 15251-6399 . Ann. TSCA User fee. 

Part I-GENERAL INFORMATION 

You must provide tile currently correct Chemical Abstracts (CA) Name of the lie" 

chemical substance . even d you claim ihe identity as confidential You maN authorize 

another person to submit chemical identity information for vou, but your submission will 

not be complete and the review will not begin until EPA receives this informatlon . A 

letter in support of your submission should reference vour TS user fee identification 

number You must submit an original and two copies of this notice including all test data . 

If you claimed anv information as confidential, a single sanitized copy must also be 

submitted 

Part lI - HUMAN EXPOSURE j -" RELEASE 

If there are several manufacture. ^ tt . 

sections A and B of this notice,, 

Part III -LIST OF ATTACF 

Attach additional sheets if th 

each continuation sheet will 

attachments . anv test data c 

notice . 

OPTIONAL INFORM/ 

You may include any i 

substance . On page L 

Pollution prevention 

substance . 

So-called "binding* 

willingness to be t 

production volume . t, . _ 

that routtnelc accompan)" the oc . __ 

Rules Except m the case of exemption app . ._ . 

where certain information provided in such notification 

the Agency approves the exemption application . checking a binding 

does not by itself 'prohibit the submrtter from later deviating from the mfonnanl,. . , 

chemical identity) reported in tile form 

CONFIDENTIALIT)' CLAIMS 

You mar claim anv mformation in thls nouce as confidenttal To assert a clalm on the 

form, mark (X) the confidential box next to the information that you claim as 

confidential . To assert a claim in an attachment, circle or bracket the information VOU 

claim as confidential . _If you claim information in the n otices as confidential you must 

also n rovide a sanitized N-erslon of the notice_ (including attachments) For additional 

instructions on claiming information as confidential, read the Instructions 'vlanual . 

® Mark (x) if any information in Ihis notice is claimed as confidential . 

TEST DATA AND OTHER DATA 

You are required to submit all test data in your possession or control and to provide 

a description of all other data known to or reasonably ascertainable bv you, if these 

data are related to the health and environmental effects on the manufacture. 

processing . distribution m commerce . use. or disposal of the new chemical 

substance. Standard literature citations mav be submitted for data in the open 

scientific literature Complete test data (tNTttten in English) . not summaries of data . 

must he submitted if they do not appear ill tile open literature You should clearl\ 

tdenufv whether test data is on the substance or on an analog Also . tile chemical 

composition of the tested material should be characterized FollowinE are 

examples of test data and other data Data should be submitted according to the 

requirements of §720 .50 of the Prematmfacture Notification Rule (40 CFR Part 

720) . 

(Check Below all\- Included in this notice) 

mimental fate data 

Ith effects data 

,vlronmental effects data 

hyslcaliChelntcal Properties* 

0 

® 

® 

® 

Yes 

Yes 

Yes 

Yes 

0 

" Other data 0 Yes 

Risk assessments 

Structure/actrvity relationships 

Test data not in the possession 

or control of the submitter 

A phvslcal and chemical properties worksheet is located on the last page of this form . 

YPE OF NOTICE (Check Onlv One) 

PMN (Prentanufacture Notice) 

INTERMEDIATE PMN (submitted m sequence with final product PMN) 

SNUN (Significant New Use Notice) 

TMEA (Test Marketing Exemption Application) 

LVl : (LoH Volume Exemption) n 40 CFR 723 .50(c)(I ) 

LOIZEX (Lott Release/Low Exposure Exemption) Ca 40 CFR 723 .50(c)(2) 

LVE Modification 

IS TtIIS A CONSOLIDATED PMN? 

LOREX Modification 

Yes 

PI of chemicals 1 

(Pienotice Communication r rc(Iuned, entei = on page 3) 

AGENCY USE ONLY 

1 i -1 EP 1 u 1 1 

r 

EPA FORM 7710-25 (Rev . 5-95) j I C? ~/ (~ c~ Replaces previous editions of 1?PA Form 7710-25. 
Part 1 -- GENERAL INFORMATION 

Section A --SUt;n-nrr>'aR tDcN~rtFICATION 

Mark () the "Confidential" box next to am Subsection Vou claini as confidential 

I a Person Name of authorized official Position 

Submitting 

Notice (in U.S .) 

Company 

Mailin2 address (number and street) 

Cit~ . State- ZIP Code 

b. Agent (if Name of authorized official Position 

applicable) 

Company 

Mailing address (number and street) 

Citti ., State. ZIP Code Telephone Area Code Number 

c . If you are submitting this notice as part of a joint submission . mark (X) tills box. 

------ 

Joint Submitter (if Name of authorized official Position 

applicable) 

Company 

Mailint_ address (number and street) 

City, State. ZIP Code Telephone Area Code Number 

2. Technical Name of authorized official Position 

Contact (in 

X U.S .) 

Compam 

Mailing address (number and street) 

City . State. ZIP Code Telephone Area Code Number 

3 If you have had a prenouce communication (PC) concerning this notice 

and EPA assigned a PC Number to the notice . enter the number Mark (X) -~ ® 

if none 

4 If you previoush submitted an exemption application for the chemical 

substance covered bv this notice . enter the exemption number assigned b% Mark (X) -~ ® 

EPA. lf you previouslNsubmttted a PMN for this substance enter the If none PMN number assttmed bc EPA (t .e withdrawn or incomptetei----i 

5 . If you have submitted a notice of Bona fide intent to manufacture or import 

for the chemical substance covered b_v this notice- enter the notice number 

i d b EPA Mark (X) -~ ® ass tnie t - if none 

I . ® Manufacture 2. ~ Import 

6 . Type of Notice - Mark (X) Only Only 3. ~ Both 

Binding Option ~ Binding Option 

Mark (X) Mark ( X) 

Confidential 

EPA FORM 7710-25 (Rcv . 5-95) Pa~e 3 
Part 1 -- GENERAL INFORMATION -- Continued 

Section B -- CHEMICAL 1DGNTIT1 INFORMATION: lbu must providc a cunenth-correct Chemical Abstracts (CA) name (it ILe substancc based on 

the ninth Collecuvc hides (4CI i (if CA nomenclature rules alid conventions 

Mark (X) the "Confidential- box next to anN item vou claim as confidential 

Complete either item I (Class I or 2 substances) or 2 (Polvmers) as appropriate. Complete all other items. 

If another person will Submit chemical identity information i2br vou (for either Item I or 2)_ mark (X) the box at the ri2hL Conflldentiiv the name . compam . and address of that person In it continuation shect El dermal 

I . Class I or 2 cherntcal substances (for definitions of class I and class'_' substances_ see the instructions Manual) 

a Class o1 substance - Marl. (X) I [_] Class I or '? [:] Class 2 

b. Chemical name (Current)\, correct Chemical Abstracts (C'A) Name that is consistent with 'hSCA Inventor \, for similar substances . 

For Class I substances a CA Index Name must be provided. For Class "_' substances either a CA Index Name or CA Preferred Namc must 

be provided which ever is appropriate based on CA 90 nomenclature rules and conventions) 

c. Please identifj which method you used to develop or obtain the specified chemical identm information reported in this notice (check oil(;) . 

Method 1 (CAS Inventory Expert Service - a copy, of the Identification ~ Method 2 (Other Source) 

report obtained from the CAS inventory Expert Services must be 

submitted as an attachment to this notice) 

d. Molecular formula and CAS Re ;istn Number (if a number already exists for the substance) I 

I 

I 

CASt;' 

e . For a class I substance. provide a complete and correct chemical structure diagram. For a class 2 substance - (1) List tile immediate 

precursor substances with their respective CAS Registn Numbers. (2) Describe the nature of the reaction or process. (3) Indicate the I 

range of composition and the typical composition (where appropriate) . (4) Provide a correct representative or partial chemical structure 

diasram. as complete as can be known. if one can be reasonabl\ ascertained . 

~fI G 1"rG l~Y J f..f FJ staE f 4 e F o7 Gi polymer. 

~ Mark (X) this hox if you attach a continuation sheet. 

hPA FORM 7710-25 (Rev . 5-95) Paee 4 
Part 1 -- GENERAL INFORMATION -- Continued ' 

Section ti -- Ci-tEa~1CAL tuc.N"rlTIt!vrOliN-tA~rION -- Continued 

2. Polvrners (hot a definition of polymer_ see the Instructiuns Manual .) Confidential 

a. Indicate the number-average weight of the lowest molecular wei2ht composition o1 the polymer you intend to manufacture 

Indicate maximum weight percent of low molecular weight species (not mcludin,_ residual monomers . reactants. or solvents) below 500 and 

below 1 .000 absolute molecular weight of that composition. 

Describe the methods of measurement or the basis for your estimates: G PC Other ®. (Specify) estimate based on GPC data 

for [ lderiv . and calculation 

i) lowest number averaec molecular weight 

ii) maximum wei,,,ht % below 500 molecular weight 

iii) maximum weight % below 1000 molecular weight 

Mark (XI this box ifvou attach a continuation sheet 

b. You must make separate confidentiality claims for monomer or other reactant identttN . composition information. and residual information. Mark (X) the 

"Confidential" box next to am item vou claim as confidential 

(1) - Provide the specific chemical name and CAS Re=istr) Number (if a number exists) of each monomer or other reactant used in the manufacture of 

the polymer. 

(2) - Mark (X) this column if entr\ in column (1) is confidential 

(3) - Indicate the typical weight percent of each monomer or other reactant in the polymer 

(4) - Mark (X) the identitv column if you want a monomer or other reactant used at two weight percent or less to be listed as part of the polymer 

description on the TSCA Chemical Substance InventorN 

(5) - Mark (X) this column if entries in columns (3) and (4) are confidential . 

(6) - Indicate the maximum weight percent of each monomer or other reactant that may be present as a residual in the polymer as manufactured for 

commercial purposes . 

(7) - Mark (X) this column if entrn in column (6) is confidential . 

Monomer or other reactant and CAS Resistrn Number Confi- Typical Identit` Confi- Maximum Confidential composition Mark (X) dential residual dential 

(1) (?) (3) (4) (5) (6) (7) 

X X X X 

X X X X 

X X X 

X X X 

°io °iu 

°~o °io 

Mark (X) this boa if vou attach a continuation sheet . 

c. Please identify which method you used to develop or obtain the specified chemical identit~ information reported in this notice (check one) . 

® Method I (CAS Inventory Expert Service - a cop), of the identification report 1:1 Method 2 (other source) 

obtained from CAS lnventor~~ Expert Service must be submitted as 

as attachment to this notice) 

d. The currently correct Chemical Abstracts (CA) name for the polymer that is consistent with TSCA Inventorv listings for similar polymers . 

e. Provide a correct representative or partial chemical structure diasram, as complete as can be known. if one can be reasonablv ascertained. 

X 

® Mark (X) this box if you attach a continuation sheet. 

EPA FORM 7710-25 (Rev . 5-95) [) ;I,-,c 5 
Part I -- GENERAL INFORMATION -- Continued 

Section B -- CHEMICAL IDENTITI INhORI11ATI0N -- Continued 

3 . Impurities 

(a) - Identil'N each unpurnv that mav be reasonablN anticipated to be present m the chemical substance as manufactured for commercial purpose. 

CAS Registr\ Number if available. Il there are unidentified impurities . enter '111videntified ." 

(h) - Estimate the maximum wet0ht °,o of each im purm If there are Unidentified impurities. estimate their total weight °/o 

Provide the 

Impurity and CAS Registn Number 

(a) 

Maximum 

percent 

(b) 

Confidential 

None known 

Mark (X) this box if you attach a continuation sheet. 

4 . Svnonvms - Enter anv chemical synonyms for the new chemical identified m subsection 1 or 2. 

- J ~ ~ 

Confi- , 

dential ' 

Mark (X) this box if you attach a continuation sheet. 

~ . Trade identification - List trade names for the neNN chemical substance identified in subsection 1 or 2. 

Mark (X) this box if vou attach a continuation sheet. X 

6. Generic chemical name - If vou claim chemical identifi as confidential . you must provide a generic name for vour substance that reveals 

the specific chemical identity of the new chemical substance to the maximum extent possible . Refer to the 

TSCA Chemical Substance Inventor), 1985 Edition. Appendix B for guidance on developing generic names. 

Alcohol Ethoxylate 

Mark (X) this box if vou attach a continuation sheet. 

7 . Byproducts - Describe anv byproducts resulting from the manufacture. processing. use. or disposal of the neNN chemical substance. Provide the CAS Reatstn 

Number if available. 

Byproduct 

(1) 

CAS Resistr} Number 

~ (2) 

Confidential 

Mark (X) thi, 

-- 

hux if you attach a continuation sheet. ---- 

- -- 

--~ 

------------- --- - 

I ;PA FORM 7710-25 (Rev . 5-95) Page 
Part I -- GENERAL INFORMATION -- Continued 

Section C -- PRODUCTION, IMPORT, AND USE INFORMATION: 

Mark (X) the "Confidential" box next to anv item vou claim as confidential . 

1 . Production volume -- Estimate the maximum production volumc during the first 12 months of production . Also estimate the mzLximum 

production volume for anp consecutive 12-month period during the first three years of production . Estimates should be on 1()0%b ,-tev~ chemical 

substance basis . For a Low Volume Exemption application . if you choose to have ~~our notice reviewed at a lower production volume than 

10 .(10(1 k<`/vr . speciiy tile volume and mark w in the binding box. lf eranted . Vou are bound to this volume 

Maximum first l2-month production (kg/yr) Maximum 12-month production (kc-,iyr) Cor,fi- Binding 

(100% new chemical substance basis) (100°rb new chemical substance basis) dential Option 

Marl: (x 

X 

2 . Use Information -- You must make separate confidentialit\ claims for the description of the categon of use . the percent of production volutne 

devoted to each categor} . the formulation of the neN substance . and other use information . Mark (X) the "Confidential" Boa next to anNitem 

you claim as confidential . 

a. (1) -- Describe each intended category of use of the nevN chemical substance bN function and application . . 

(2) -- Mark (X) this column if entrv column (I ) is confidential business information (C131) . 

(3) -- Indicate your willingness to have the information provided in column (I ) binding . 

(4) -- Estimate the percent of total production for the first three years devoted to each categor\ of use . 

(5) -- Mark (X) this column if entry in column (4) is confidential business information (CBI). 

(6) -- Estimate the percent of the new substance as formulated in mixtures . suspensions. emulsions . solutions . or gels as manufactured for 

commercial purposes at sites under your control associated with each category of use . 

(7) -- Mark (X) this column if entry in column (6) is confidential business information (CBI). 

(8) -- Indicate % of product volume expected for the listed "use" sectors . Mark more than one box if appropriate . Mark (X) to indicate 

your willingness to have the use type provided in (8) binding . 

(9) -- Mark (X) this column if entr_v(ies) in column (8) is (are) confidential business information (CBI). 

Cate2on of use (1) CBI ffindmg Produc- CBI % m CBI % of substance expected per use CBI 

Opuon 

tlon °o Form- (8) 

(bN function and application i .e . a dispersive dve nla't (') ulation Site- Con-* Indus- Com- Binding 

for finishing polyester fibers) (2) (') (4) (~) (6) (7) limited sumer trial mercial Option - (Q) 

Site limited, isolated, chemical 

intermediate 

X % X n/0 X X X 

o- a . 

o, o, 

0 0 

o- o 

n n 

* If you have identified a `consumer use. please provide on a continuation sheet a detailed description of the use(s) of this chemical substance m consumer products . 

III addition include estimates of the concentration of the neN chemical substance as expected in consumer products and describe the chemical reactions bN which this 

substance loses its identit-v in the consumer product. I, 

E]Mark (X) this box if v_ ou attach a continuation sheet. 

b. Generic If you claim anc category of use description in subsection 2a as confidential . enter a generic description of that categorN . Read the 

use Instructions Manual for examples of generic use descriptions . description 

~ Mark (X) this box if vou attach a continuation sheet. 

3. Hazard Information -- Include in the notice a copy of reasonable facsimile of am hazard warning statement. label. material safet\ data sheet- or other BindmL 

information which will be provided to anv person who is reasonably likely to be exposed to this substance regarding protective equipment or practices optnlark (c) 

for the safe handing. transport. use, or disposal of the new substance. List in part III hazard information you include. 

® Ivlark (X) this boa if you attach hazard information. 

EPA FORM 7710-25 (Rcv . 5-95) Pagc 7 
Part II-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE 

~ Section A -- INDUSTRIAL SITES CONTROLLED BY THE SUf3MITTER Marl. (X) the -Confidential- box next to am Item vou 

claim as confidential 

Complete section A for each tvpc of manufacturc_ processing . Or use operation involvinc the nc\\ chemical substance at industrial sites vou 

control . Importers do not have to complete this section for operations outside the U.S . : however. you mm still have reporting requirements if 

there are further industrial processing or use operations after import . You must describe these o perations . See instructions manual 

1 . Operation description Confia. Identity -- Enter the identits of thc site at which the operation Will occur . dential 

Name 

Site address (number and street) 

City, County, State, ZIP code 

If the same operation will occur at more than one site . enter the number of sites . ldentifN the 

additional sites on a continuation sheet. and if am of the sites have significantlN different 

production rates or operations. include all the information requested in this section for those 

sites as attachments . 

® Mark (X) this box if vuu attach a continuation sheet. 

b . Type -- 

Mark (X) ®Manufacturing E]Processinc Ouse 

c. Amount and Duration -- Complete 1 or 2 as a propriate 'i 

Maximum IC-,/batCll (100°.o new Chermcal Hours/batch Batches/year X 

substance) 

l . Batch 

Maximum kg/bateh (loo% ne« chemical Hours/batch Batches/year 

substance) 

2. Continuous 

d Process description ® Mark (X) to indicate vour willingness to have ~~our process description binding. 

(1) Diagram the major unit operation steps and chemical conversions. Include interim storage and transport containers (specify- e.g . 5 gallon pails. 55 2allon 

drum . rail car. tank truck. etc.) . 

(2) Provide the identity . the approximate weight (b) k2/dav or kg/batch on a 100n/o neN\ chemical substance basis) . and entn point of all startima materials and 

feedstocks (including reactants. solvents . catalysts. etc.) . and of all products . recycle streams. and wastes . Include cleaning chemicals (note frequency if not 

used dail~ or per batch.) . 

(3) Identifi by number the points of release. including small or intermittent releases . to the environment of the neN chemical substance. 

Su,ne attached manufacturing did-gram. 

® Mark (X) this box it )()u attach a continuation sheet. 

EPA FORM 7710-25 (Rev . 5-93) Page 8 
p. 9 

Part II-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE -- Continued 

Section A -- INDUSTRIAL SITES CONTROLLED BY THE SU(3MITTGR -- Continued 

'' . Occupational Exposure--You must make separate confidentialtt\ claims for the description of worker activm . phvsical form of the ne« chemical substance. 

number of works exposed. and duration of actuvrn Marl. (X) the -Confidential'" box next to am nem vou claim as confidential . 

(1) --Describe the activities (i .e baL dumping. tote filling. 

unloading drums . samplinW1 . clcantnL. ctc. ) In Which workcrs mm ~ be exposed to the substance . 

(2) --Marl. (X) this column it entrN in column ( I ) is confidential business information With 

(3) --Describe am protective equipment and enameerin~s controls used to protect workers 

(4) and (6) -- Indicate vour Nvillin=ness lo have the Information provided m column (3 ) or (5 ) binding 

(5) --Indicate the phvstcal fonn(s) of tile nev+ chemical substance (ca . solid . cr~~stal . Lranule, powder. or dust l and °/b new chemical substance (ifpart of a mixture) 

at the time of exposure . 

(7) --Mark (X) this column if entn in column (5) is confidential business information (CB11. 

(8) --Fstmiate the maximum number of workers involved in each activrt\ lot all sites combined 

(9) --Marl. (X) this column if entry in column (b) is confidential business information (C131) . 

10) and (1 I ) -- Estimate the maximum duration of the activith_ for am worker in hours per da\ and davs per year 

(12) -- Mark (X) this column if entries in columns (1(1) and (1 1) arc confidential business information (CBI). 

Worker actrvm CBI Protecuvc Equipment n,nd~n_' Phystcal formstsr u,nd .n, CBI # of CBI Maximu 

m 

duration C131 

(i .e . . bau dumptn(1 . filling, drums) Lncineermg Controls opnon and % . nekN Option 

M rk (,) 

Worker Hrsidan DaYs,Yr 

Mark (x) substance a 

(I ) (2) (3) (4) (,) (6) (7) Expose (`t) (lO) (11) (t_) 

d 

(F) 

X glasses, apron and Liquid X 

rubber gloves 

X glasses, and rubber Liquid 1 X 

gloves 

~ Mark (X) this box if vou attach a continuation sheet. 

3. Environmental Release and Disposal -- )'Oil must make separate confidentiaht` claims for the release number and the amount of the new chemical substance 

released and other release and disposal information. Mark (X) the '*Confidential"" box next to each item ,,on claim as confidential . 

(I ) --Enter the number of each release point identified in the process description. part 11. section A. subsection 1 d(3) . 

(2) --Estimate the amount of the new substance released (a) directlN to the environment or (b) into control technolo-LN(in kg/da\or kg/batch) . 

(3) --Mark (X) this column if entries in columns (1) and (2) are confidential business information (CBl) . (4) -- ldentifj' the media (stack air. fugitive air (optional-see Instruction Manual) . surface water . on-site or off-site land or incineration. POTVr'_ or other (specify)) lo 

which the nev, substance will be released from that release point. 

(5) --a. Describe control technoloa) . if anc_ and control efficiencc that 'will be used to limit the release of the nevN substance to the environment. For releases 

disposed of on land . characterize the disposal method and state whether it is approved for disposal of RCRA hazardous waste . On a continuation sheet . for 

each site describe am additional disposal methods that will be used and whether the waste is subject to secondan or tertian on-site treatment. b. Estimate 

the amount released to the environment after control technolo=v (in kelda~~) . 

(6) --Mark (X) this column if entries in columns (4) and (5) are confidential business information (CB1). 

(7) --Identifj' the destination(s) of releases to water. Please supplv NPDES (National Pollutant Discharge Elimination System) numbers for direct discharges or 

NPDES numbers of the POTNA(Pubhcly Owned Treatment Works) . Mark (X) if the POTVt' name or NPDES #I is confidential business information (C BI). 

Release Amount of new substance CB l Media of Control technolon and efficiency (you ma\ wish to optionall\ attach efficiencN data) Cut 

Number released release 

(1) (2a) - (2b) e_,, sack- Binding 

(3) (4) (5a) Mark (X) (5b) (6) 

1 a 0 85 water Onsite 1NWTP X 8.5 

1 b 0 8.5 water Offsite WW-FP X 0.85 

(7) Mark (X) the ® pOTW provide name(s) below: CBI El Navigable ~ Other - Specifj provide NPDES ir CBI 

destination(s) of waterway X 

releases to water. 

Mark (X) this box if Nou attach a continuation sheet. 

EPA FORM 7710-25 (Rev . 5-95) PaLe 9 
p. 10 

Part II-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE -- Continued 

Section B -- INDUSTRIAL SITES CONTROLLED BY OTHERS 

Complete section B for typical processtng or usc operations involving the nc%\ Chemical substance at sites vou do not control importers do not have to complete tills 

section (of operations outside tile U.S . : howeve . you must report am processing or use activities after import . Scc the instructions Manual. Coniplete a separaic 

section B,1or each llipe qJprocessm ;, or usc operarrnn urrolvur,> the Iletr chenucal .cuhstance If the same operation is performed at more than one sttc describe the 

typical operation common to these sites . Identlf\ additional sites Oil a Continuation sheet 

1 . Operation Description --'1 o claim information in this section as confidential . circle or bracket life specific mfonnadon that v_ ou claim as confidential . 

(I ) -- Diagram the major unit operation steps and chemical Conversions. Including interim storage and transport containers (specit) - c .= . 5 -gallon oails. 55 gallon 

drums. rail cars . tank trucks. etc) On the dta~ranl_ tdentifv b_v letter and briefl~ describe each worxer activtt\ (2) -- Provide the identnN . the approximate weight 

(bN kJda\ or kg/batch . on an I()()°~~ nesV chemtcal substance basis)_ and entn point of all teedstocks (including reactants_ solvents and catalysts_ etc) and all 

products . recycle streams. and wastes . Include cleaning chemicals (note lrequencv if not used dail) or per batch) . (3) -- identify by number the points of release. 

Including small or intermittent releases . to the environment of the ne%\ chemical substanec. (4) Please enter the # of sites (remember to identilj the locations of 

these sites on a continuation sheet) : 

(I 

9 of sites 

hl©t applicable, site limited interme late. 

® Mark (X) this box if vou attach a continuation sheet. 

2. Worker Exposure/Environmental Release 

(I ) --From the diagram above. provide the letter for each worker activity . Complete 2-8 for each worker activitv described. 

(2) -- Estimate the number of workers exposed for all sites combined . 

(4) --Estimate the itypical duration of exposure per worker in (a) hours per day and (b) days per year . 

(6) --Describe plivsical fonn of exposure and % new chemical substance (if in mixture) . and am_ protective equipment and ensineerin_ controls . if any. used to 

protect workers . 

(7) --Estimate the percent of the new substance as formulated when packaged or used as a final product. ' 

(9) --From the process diagram above. enter the number of each release point. Complete 9-13 for each release point identified . 

(10) -- Estimate the amount of the new substance released (a) directlv to the environment or (b) into control technology to the environment (in kJday or kg/batcll). 

(12) -- Describe media of release i .e . stack air . fitizitive air (optional-see Instructions Manual) . surface water . on-site or off-site land or incineration . POTIA' . or other 

(specify) and control technology. if anm . that will be used to limit the release of the new substance to the environment. 

(14) -- identifj byproducts which may result from the operation. 

_ (3). (5). (S)_ (11) . (13) and (15) -- Mark (X) this column if anv of the proceeding entries are confidential business information (CBI). 

Letter r of CBI Duration CBI Protective Equip . / °o In CBI Release Amount of CBl Media of Release CBI 

of Workers of Eneineeritta Controls/ Fonn- Number New &. Control 

Act- Exposed Exposure ~ ~ Physical Foml and ro o- ulauon Substance Technology 

ivitv nexN substance Released 

(1) (2) (3) (4a) (4b) (5) (6) (7) (8) (91 (IOa) (IOb) (I11 (12) (13) 

(14) -- Byproducts : (15) ', 

NONE ' 

E]Mark (X) this box if vou attach a continuation slleet_ 

EPA FORM 7710-25 (Rev . 5-95) Pa2e 1 0 
p. 11 

OPTIONAL POLLUTION PREVENTION INFORMATION 

To claim information in this section as confidential circle or bracket the specific information that you claim as confidential . 

In this section you may provide information not reported elsewhere in this form regarding your efforts to reduce 

or minimize potential risks associated with activities surrounding manufacturing. processing . use and disposal 

of the PMN substance. Please include new information pertinent to pollution prevention . including source 

reduction, recycling activities and safer processes or products available due to the new chemical substance. 

Source reduction includes the reduction in the amount or toxicity of chemical wastes by technological 

modification . process and procedure modification . product refonnulation. raw materials substitution ; and/or 

inventory control . Recycling refers to the reclamation of useful chemical components from wastes that would 

otherwise be treated or released as air emissions or water discharges . or land disposal . Descriptions of pollution 

prevention . source reduction and recycling should emphasize potential risk reduction subsequent to compliance 

with existing regulatory requirements and can be either quantitative or qualitative . The EPA is interested in the 

information to assess overall net reductions in toxicity or environmental releases and exposures. not the shifting 

of risks to other environmental media or non-environmental areas (e .g ., occupational or consumer exposure) . In 

addition. information on the relative cost or performance characteristics of the PMN substance to potential 

alternatives may be provided. 

All information provided in this section will be taken into consideration during the review of this 

substance. See Instructions Manual and Pollution Prevention Guidance manual for guidance and 

examples. 

Describe the expected net benefits . such as (1) an overall reduction in risk to human health or the environment: (2) a reduction in the 

volume manufactured; (3) a reduction in the Qeneration of waste materials through recycling. source reduction or other means; (4) a 

reduction in potential toxicity or human exposure and/or environmental release: (5) an increase in product performance, a decrease in 

the cost of production and/or improved operation efficiency of the new chemical substance in comparison to existing chemical 

substances used in similar application: or (6) the extent to which the new chemical substance may be a substitute for an eaistina 

substance that poses a areater overall risk to human health or the environment. 

EPA FORM 7710-25 (Rev . 5-95) PaLe I 1 
Part III -- LIST OF ATTACHMENTS 

Attach continuation sheets for sections o[ the form and test data and other data (including physical/chemical properties and 

structure/activitv information) . and optional information after this page . Clearly identiiN the attachment and the section of the form to 

which it relates . if appropriate . Number consecutivel\ the pages of the attachments . In the column belo\~~ . enter the inclusive page 

numbers of each attachment. 

Mark (X) the "ConfidentiaF box next to an\ attachment name Vou claun a, confidential . Read the Instructions Manual for guidance on 

hov< to claim am information in an attachment as confidential . YOU must include with the sanitized cop~ of the notice form a sanitized 

version of an\ attachment in which \10u claim information as confidential . _ 

Attachment name Attachment 

page number(s) 

Confidential 

1-Material Safetv Data Sheet MSDS _ 

14-19 X 

2-CAS IES Documents (from previous ) 20-25 X 

3-Industrial Sites Controlled by Submitter- Manufacturing Diagram 26-27 X 

4-Industrial Sites Controlled by Submitter-Processing Diagram 28-29 X 

5-GPC Chromatograms (based on [ ] deriv.) 30-33 X 

6-Fish, Acute Toxicity Static 96hr 34-48 X 

7-Biodegradability OECD 301 E 49-55 X 

8-COD/DOC 56-68 X 

9-Bacterial toxicity 69-73 X 

~ Mark (X) this box if vou attach a continuation sheet. Enter the attachment name and number . 

lil'A FOR1A 7710-25 (Rev . 5-95) Pagc 1 2 
p. 13 

PHYSICAL AND CHEMICAL PROPERTIES WORKSHEET 

'to assist EPA's revicxN of physical and chemical properties data . please complete the following workshect lor data vou provide and 

include it in the notice. ldentifN ' the propert\ measured the page of the notice on which the propem appears. the valuc of tile propertN . 

the units in which the propem is measurcd (as necessarv) . and whether or not the properh is claimed as confidential . The plivsical 

state of the neat substance should be provided These measured properties should he lor the ncat (10(1°  pure) chemical substance. 

Properties that arc measured for mtxtures or formtdauons should be so noted (°- PMN substance m _) You are not required to 

subnut this worksheet ; however. EPA stron2l\ recommends that vou do so . as It Will sunplif.N revien and ensure that confidential 

information is properlv protected. )'Oil should suhmit this worksheet as a supplement to YOU- submission of test data This worksheel 

is not a substrlutc for submission of test data 

---- Properth Marl. Page Value Measar-,-d oi C-11- 

(X) nlnlber Estimate denual 

MarA (X) 

(al pI m ided 

(bl (c) (MorE) 

M 

Phvsical state of neat substance (s) X (1) - (g) 

Vapor pressure 

(a,; Temperature °C 

Densitv/relative density U/ctn3 M X 

Solubility 

g Temperature °C 

Solvent g/L 

Solubilitv in water ca Temperature °C E/L 

Melting temperature °C 

Boilina / sublimation temperature Ca,- ton- pressure OF 

Spectra 

Dissociation constant 

Particle size distribution 

Octanol / water partition coefficient 

Hem-v's Law constant 

Volitalization from water 

Volitalization from soil 

H(2 concentration 

°F M X 

Flammabilitv 

Ex lodability 

Adsorp tion / coefficient 

Other - Specify 

mPa*s @ ° C M X 

Viscosity 

Thermal Decomposition Temperature 

ATTACHMENT 

PAGE 13 
MATERIAL SAFETY DATA SHEET 

L J 

Page 1 

Substance key: SXR004863 Revision Date : 01I06/2009 

Version : - I USA Date of printing :01I0612009 

Section 01 - Product Information 

Identification of the 

company : 

Trade name: 

CAS number: 

F . - 

Information of the substancelpreparation : 

Emergency tel . number: +1 800-424-9300 CHEMTREC 

1 

Primary product use: 

Chemical family : `Fatty alcohol polyglycol ether 

Section 02 - Composition information on hazardous in 

Hazardous ingredients : 

redients 

Component CAS-no. (Trade secret Concentration 

_ no .) 

100% 

Section 03 - Hazards identification 

Emergency overview : Clear liquid . 

Severe eye irritant, may cause loss of sight. 

Toxic to aquatic organisms 

Expected Route of entry : 

Inhalation : Vapors and/or mists are probably irritating to 

respiratory passages, nose, and throat . 

Skin contact: Not expected to be irritating to skin. 

The acute dermal toxicity of this product is greater 

than 1000 mg/kg. This product is not toxic by skin 

absorption . 

Eye contact: Causes moderate to strong eye irritation . 

Ingestion : The acute oral toxicity of this material is between 

500 and 5000 mg/kg . Relative to other materials, 

this material is classified as slightly toxic by 

ingestion . 

Skin absorption : no 

Known effects on other illnesses : None known. 

Listed carcinogen : [ARC: No 

NTP: No 

OSHA: No 

Other: No 
~IATERIAL SAFETY DATA SHEET 

Page ? 

Substance key: SXR004863 Revision Date: 01106/2009 

Version : - l USA Date of printing :01/06/2009 

HMIS: 

Health : 2 Flammability : 1 Reactivity : 0 Personal protection : D 

~ Section 04 - First aid measures 

After inhalation : 

Get victim to fresh air . Give artificial respiration or oxygen if breathing has stopped. Get 

prompt medical attention . Do not give fluids if victim is unconscious. 

After contact with skin : 

Wash thoroughly with soap and water for 15 minutes. If skin irritation occurs, seek medical 

attention . 

After contact with eyes : 

Flush thoroughly with water for 15 minutes . Get immediate medical help . 

After ingestion: 

Seek medical attention immediately . 

Advice to doctor / Treatment: 

None known. 

Section 05 - Fire fi hg ting measures 

Flashpoint: 374 °F 

Method : DIN/ISO 2592 (open cup) 

Lower explosion limit : not tested. 

Upper explosion limit: not tested. 

Self ignition : Not applicable 

Ignition temperature : not tested . 

Hazardous combustion products : 

In case of fires, hazardous combustion gases are formed: Carbon monoxide (CO) 

Extinguishing media: water spray jet 

foam 

carbon dioxide 

dry powder 

Special fire fighting procedure : 

Use self-contained breathing apparatus and full protective clothing . 

~ Section- 06 - Accidental release measures 

Steps to be taken in case of spill or leak : 

Contain spill . Ensure adequate ventilation and wear appropriate personal protective 

equipment. Collect onto inert absorbent . Place in sealable container. Do not allow to 

contaminate water sources or sewers . 
MA i F.Ri A T , q A EETY DATA SHEET 

L 

Substance key: SXR004863 

Version : - /USA 

Section 07 - Handlin and stora e 

Page 3 

Revision Date: 01/06/2009 

Date of printing :01/06/2009 

Advice on safe handling: 

Wash thoroughly after handling. 

Sensitive to frost : If product becomes opaque, thickening, or frozen it can be slowly thawed 

at room temperature and ready for use after a short stirring time . Avoid skin and eye contact . 

Further info on storage conditions : 

Keep containers closed. 

Section 08 - Exposure controls / personal protection 

No level has been established by OSHA, NIOSH, ACGIH . 

Respiratory protection: 

Hand protection: 

Eye protection: 

Other protective equipment : 

Section 09 - Ph 

Form : 

Color: 

Odor: 

pH: 

If airborne concentrations pose a health hazard, become 

irritating, or exceed recommended limits, use a NIOSH approved 

respirator in accordance with OSHA respiratory protection 

requirements under 29CFR1910 .134. 

PVC, Nitrile, Or Neoprene. 

Chemical splash goggles. 

Clothing suitable to prevent skin contact . 

Y sical and chemical 

Solubility in water: 

Soluble in . . . : 

Density: 

Pourpoint : 

Boiling temperature : 

Vapor pressure: 

Bulk density : 

Relative vapor density : 

Partitioning coef. 

octanol/water: 

prop erties 

Liquid 

cloudy 

slight, original odour 

6 - 8 (20 °C, 50 g/1) 

Method : DIN 53996 

Ethanol/Water 1 :1 

(20 -C) 

soluble, turbid 

fat 

not tested . 

0.95 g/cm3 (50 °C) 

Method : DIN 51757 

approx . 73 °F 

Method: DIN/ISO 3016 

not determined 

not tested . 

Not applicable 

not tested. 

not tested. 
p. 17 

MATFRIAL SAFETY DATA SHEET 

-J 

Page 4 

Substance key : SXR004863 Revision Date: 01/06/2009 

Version : - / USA Date of printing :01/06/2009 

Viscosity / (dynamic) : approx. 25 mPa.s (50 °C) 

Method : DIN 53015 

Viscosity / (kinematic) : not tested . 

Section 10 - Stability and reactivit~ 

Thermal decomposition : No decomposition if used as prescribed . 

Hazardous Polymerization : Will not occur. 

Conditions to avoid : None known. 

Section 11 - Toxicolo g ical information 

Product information: 

Acute oral toxicity : LD50 500 - 2,000 mg/kg (rat) 

Source: CESIO 

Acute inhalation toxicity: not tested . 

Acute dermal toxicity : not tested . 

Skin irritation : non-irritant (rabbit) 

By analogy with a similar product. 

Eye irritation : irritant - risk of serious damage to eyes (rabbit eye) 

By analogy with a similar product. 

~ Section 12 - Ecological information 

Product information : 

Biodegradation : > 80 % 

Method : OECD 302 B 

Biodegradation : 96 % (28 d) 

readily degradable 

Method : OECD 301 E 

Fish toxicity : LC50 1 .6 mg/1 (96 h, zebra fish) 

Method : OECD 203 

Bacteria toxicity : EC50 98 mg/l 

Method : OECD 209 

Dissolved organic carbon 650 mg/g 

(DOC) : 

Chemical oxygen demand 2,275 mg/g 

(COD): 

Section 13 - Disposal considerations 
MATERIAL SAFETY DATA SHEET 

-J 

Substance key: SXR004863 

Version : - / USA 

Waste disposal information : 

Recommended disposal is by incineration in approved facilities . 

RCRA hazardous waste : 

No -- Not as sold. 

Section 14 - Trans p ort information 

DOT Regulation : 

Proper shipping name: 

Hazard class: 

Packing group: 

UN/NA-number : 

Primary hazard class : 

Technical Name : 

IATA 

Proper shipping name: 

Class: 

Packing group : 

UN/ID number: 

Primary risk : 

Remarks: 

Hazard inducer(s) : 

IMDG 

Proper shipping name: 

Class : 

Packing group: 

UN no. : 

Primary risk : 

Hazard inducer(s): 

Marine pollutant: 

EmS : 

_-_J 

Page 

Revision Date : 01/06/2009 

Date of printing :01/06/2009 

Environmentally hazardous substances, liquid, n.o.s . 

9 

III 

U N 3082 

9 

Fatty alcohol polyglycolether 

Environmentally hazardous substance, liquid, n .o .s . 

9 

III 

U N 3082 

9 

Shipment permitted 

Fatty alcohol polyglycolether 

Environmentally hazardous substance, liquid, n .o .s . 

9 

III 

UN 3082 

9 

Fatty alcohol polyglycolether 

Marine Pollutant 

F-A S-F 

Further information : 

Not regulated for surface or air transportation in 

gallons (liquid) or 882 pounds (solid) . Section 15 - Re ulator information Y 

non-bulk containers under 119 

TSCA Status : 

One or more of the components of this product is not listed on the Toxic Substances Control 

Act (TSCA ) Inventory . The product is thus sold under the restriction that it is only for use in 

research and development . This product must be used under the supervision of a technically 

qualified individual capable of understanding its potential hazards . 

SARA (section 3111312) : 

Reactive hazard : 

Pressure hazard : 

Fire hazard : 

I mmediate/acute : 

Delayed/chronic: 

no 

no 

no 

yes 

no 
p. 19 

r 

O -J 

MATERIAL SAFETY DATA SHEET 

t Page 6 

Substance key: SXR004863 Revision Date : 01/06/2009 

Version : - /USA Date of printing :01/06/2009 

SARA 313 information : 

This product is not subject to SARA Title III Section 313 reporting requirements under 40 CFR 

372. 

Clean Water Act: 

Contains no known priority pollutants at concentrations greater than 0 .1 %. 

[Section 16 - Other information 

Label information: 

WARNING! 

CAUSES EYE IRRITATION THIS MATERIAL IS TOXIC TO AQUATIC ORGANISMS. 

This product is not in the TSCA inventory . It is provided only for research and 

development under the supervision of an individual technically qualified to understand 

its potential hazards . 

Avoid breathing vapor. Avoid contact with eyes, skin and clothing . Keep container 

closed, when not in use . Use ventilation and/or respiratory protection in order to keep 

exposure at a minimum. Wash thoroughly after handling . Avoid run-off into sewers 

leading to water ways. 

In case of EYE CONTACT, flush with water for 15 minutes while holding eyelids open. 

Seek immediate medical attention . SKIN CONTACT: Wash thoroughly with soap and 

water. If IRRITATION develops, call a physician promptly . Wash clothing before reuse. 

If INHALED, remove individual to fresh air . If NOT BREATHING, give artificial 

respiration . If breathing is difficult, give oxygen . Call a physician . INGESTION: Seek 

medical attention immediately . 

This information is supplied under the OSHA Hazard Communication Standard, 29 CFR 

1910 .1200, and is offered in good faith based on data available to us that we believe to be 

true and accurate. The recommended industrial hygiene and safe handling procedures are 

believed to be generally applicable to the material . However, each user should review 

these recommendations in the specific context of the intended use and determine whether 

they are appropriate for that use . No warranty, express or implied, is made regarding the 

accuracy of this data, the hazards connected with the use of the material, or the results to 

be obtained from the use thereof . We assume no responsibility for damage or injury from 

the use of the product described herein. Data provided here are typical and not_~intended 

for use as product specifications . (R) and TM indicate trademarks c'~ , 

business partners or suppliers . `-- -' 
~'~Y s~r 

Inventory Expert Sel-vice 

A, division o~ the Americarn Chemical Sodety Phone: SOD-63 I-1884, 614-447-3870 

Fax:614-447-3747 

E-mai! : answersCc,ca s .ot-g 

Web: www.cas .orqiproducaslclient' 

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IFS-0:~7rR D:',P~1'3EF : =C"~ 83° 

EEGIST~° b~I!~EF : IQo~ Rey~s~e~ea 

G%; 1NDE:_ NA?~~LlPlease print the abozre CA index Name on the appropriate page of your F 

if this box is checked, CAS has made correction(s) marked .in red to 

your IE5 order . Please make the same co=rection(s) to your PPtA7 be-fore 

submittinc it to the EPA . 

CAS - 2S40 Olenangy River Road " FO. Bol< 33-13 " Co!umbus, Ohio 43210-0339 " USA 
1-~hzSF,CP_ 10 OF i-' RJCIS'?'PY CO??1F.TGI,'I 2C07 ACS Or: 

' STRUC'~'L'fiE, DI7aMIE =S NOT 
p. 22 

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Inven, tarv -Expert Serv.1-Ce 

I-, disdslon of fhe American Ghv_mical Society Phone: $OG-G'1-1gg9 61t,Wt,47_3II70 

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A CA index Name is the standard output.for, substances submi:ted to the CAS Inventory Lxpar` Service . If your ' 

substanc~e has'an er%sting CAS Fegis,nNurrber¢, It will also be provided ic you . 1T you would like to have a new CAS 

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Note : If you requesl assionment of a CAE~3 Registry Number lo,, a substa,nce not already present ii~ the 

CAS Registry SysieRl; that assignment will make your substance, publicly available in ~tYie CAS Replst{ya" File . 

Customer identity Intormation will be kept confidential . If yau Wish 10 malntaln cornfidentEaFity for your 

substance's identity, you should request only e CA index, Name and not a hew CAS Registry Number. ' . 

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CAS - 2540 Dipntangy River Road - P.C . Bo;: 2,343 -Columbus, OH 4^321o-o334 - USA 

0 /2,)()( TUE 1,i :10 J1X/HX ~~[j ~080- j~ 7 / 1 _` `
p. 23 

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6, Gcncric cncmiczIn~e - L`you ciainf cLemiaal identi:i~ as oonRd=teJ, you mmstprovide a ganenic mine for you:sabstanoe that znvceU 

tbceaeciScehr.mic~~ideutytyoftheuw~cLentical5liostavec~tbc ,~Y ;~,~*r cr.tcn:pes~h RsftrtoT21P" 

,-cn e r" p,minal 5'linStltlBp~ Tnvca~tAr~, X9Ed rci,~or~ Append'v: B fo : gtadvice oa deveiopi>k; generic na,rz~,GS . 

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7. Byproducis - Descdbc au5" 'oy" pzoducts zcnulibag fiom th~; manufaeture, nrocessiuo, usc, oz dispoosil o£tiuc ;.,cw cmc>picel snbsmace . Yraviae fac'CqS 

w~unbW ifecviinble. ' ' 

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)3ypzoduct ~ Gh.S k~egistn" Nunabor + . rn , r2 I a~s~l 

, 

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, . . . . . . I . 

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Marl: (?a This box. if von auachH ccntinuaticn shG--- 

P,1',A. ; c:,')tA4 7710 25 (Re`,. 5- .95) Pngc G 

07!1 7/~~oc.-r rui . 13 :4 ~ ~TX" ~ 1a0 ~090~ ~;; 
f)~~/ 17/200Y IUF. 'I "" .4 0 [1 :~~r, F;iS HO JoDUj PI ~)1~~~ 
p. 26 

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p. 27 

n 

n 

a: 
Part II-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE 

Section A -- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER Mark (X) the "Confidential" box next to anv item you 

claim as confidential 

Complete section A for each type of manufacture . processing . or use operation involving the new chemical substance at industrial sites you 

control . Importers do not have to complete this section for operations outside the U.S . : however . you may still have reporting requirements if 

there are further industrial processing or use o perations after import . You must describe these op erations . See instructions manual 

1 . Operation description Confia v -- Enter the identitv of the site at which the op eration will occur. . Identit dential _ 

Name 

Site address (number and street) 

City, County, State, ZIP code 

If the same operation will occur at more than one site . enter the number of sites . Identify the 

additional sites on a continuation sheet . and if any of the sites have significantlN different 

production rates or operations. include all the information requested in this section for those 

sites as attachments . 

® Mark (X) this box if vou attach a continuation sheet . 

b. Type -- 

Mark (X) OManufacturing ®Processing Ouse 

c. Amount and Duration -- Comp lete 1 or 2 as a ro riate 

Maximum kg/batch (1o0% new chemical Hours/batch Batches/year 

substance) 

1 . Batch 

Maximum kg/batch (100°., ne, chemical Hours/batch Batches/year ', 

substancel 

2_ Continuous 

d. Process description ~ Mark (X) to indicate your willingness to have your process description binding . 

(1) Diagram the major unit operation steps and chemical conversions. Include interim storage and transport containers (specify- e.- . 5 siallon pails. » gallon 

drum. rail car, tank truck. etc.) . 

(2) Provide the identity , the approximate -,ti~eight (by kg/day or kg/batch on a 100% new chemical substance basis) . and entry point of all starting materials and 

feedstocks (including reactants. solvents . catalysts . etc .) . and of all products. recycle streams . and wastes . Include cleaning chemicals (note frequency if not 

used daily or per batch .) . 

(3) IdentiN by number the points of release . including small or intermittent releases, to the environment of the neH chemical substance . 

See attached processing diagram. 

® Mark (X) this box if you attach a continuation sheet. ATTACHMENT 

PAGE 
p. 29 

Part II-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE -- Continued 

Section A -- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER -- Continued 

2. Occupational Exposure -- You must make separate confidentiality claims for the description of worker acttvit) . phvsical form of the neNc chemical substance. 

number of works exposed. and duration of activity . Mark (X) the "Confidential" box next to am item you claim as confidential . 

(1) --Describe the activities (i .e . bag dumping, tote filling. unloading drums. samphng. cleaning . etc.) m which workers mav be exposed to the substance. 

(2) --Mark (X) this column if entry m column (1) is confidential business information (CB1). 

(3) --Describe any protective equipment and engineering controls used to protect workers. 

(4) and (6) -- Indicate vour willingness to have the information provided m column (3) or (5) binding . 

(5) -- Indicate the physical forni(s) of the new chemical substance (e .g . solid: crystal. granule, powder . or dust) and °o item, chemical substance (if part of a mixture) 

at the time of exposure . 

(7) --Mark (X) this column ifentrv in column (*)) is confidential business information (CBI). 

(S) --Estimate the maximum number of workers involved in each activit\ for all sites combined . 

(9) --Mark (X) this column if entrc in column (S) is confidential business information (CBI). 

(10) and (1 1 ) -- Estimate the maximum duration of the activitN for am worker in hours per day and davs per year . 

(12) -- Mark (X) this column if entries in columns (10) and (11) are confidential business information (CBI) 

Worker activitv CBI Protective Equipment' Binding Plrvsical fornns(s) Bmdmp CBI t? of CBI Maxnmu duration CBI 

- m 

(i .e ., bag dumping, filling drums) Enaineering Controls Option and ',a neH oPim Worker HrsidaN Davs~.\ r 

Mark (r) Mark (x) substance 

(1) (') (3) (4) (') (6) (~) Expose (9) (l0) (I1) (t2) 

d 

(s) 

X Splash gogs, apron and Liquid X 

rub glvs&respirator 

X glasses, and rubber Liquid X 

gloves 

~ Mark (X) this box if vou attach a continuation sheet . 

3 . Environmental Release and Disposal -- You must make separate confidentiality claims for the release number and tile amount of the new chemical substance 

released and other release and disposal information . Mark (X) the "Confidential'- box next to each item vou claim as confidential . 

(1) -- Enter the number of each release point identified in the process description . part 11 . section A. subsection l d(3) . 

(2) --Estimate the amount of the new substance released (a) directlv to the environment or (b) into control technologv (in kg/day or kg/batch) . 

(3) --Mark (X) this column if entries in columns (1) and (2) are confidential business information (CBI) . 

(4) --Identifj~ the media (stack air, fu2itive air (optional-see Instruction Manual) . surface water- on-site or off-site land or incineration, POTR'. or other (specifi~)) to 

which the new substance will be released from that release point. 

(5) --a. Describe control technology, if an), and control efficiency that will be used to limit the release of the new, substance to the environment . For releases 

disposed of on land, characterize the disposal method and state whether it is approved for disposal of RCRA hazardous waste . On a continuation sheet . for 

each site describe all), additional disposal methods that will be used and whether the waste is subject to secondary or tertiary on-site treatment. b . Estimate 

the amount released to the environment after control technologY (in kg/day) . 

(6) --Mark (X) this column if entries in columns (4) and (5) are confidential business information (CBI) . 

(7) --Identify the destination(s) of releases to water . Please supply NPDES (National Pollutant Discharge Elimination System) numbers for direct discharges or 

NPDES numbers of the POTVt' (Publiclv Owned Treatment Works) . Mark (X) if the POTW name or NPDES #( is confidential business information (CBI). Release Amount of new substance CBI Media of Control technolo~~~ and efficiencv (you map wish to optionally attach efficiency data) CBJ 

Number released release 

(1) (2a) (2b) 

(3) 

eg aackarr 

(4) (5 a) 

Bvmding 

Mark (x) (Sb) (6) 

1 a 0 100 Water Onsite VWVTP X 10 

1 b 0 10 Water Offsite V11WTP X 1 

(7) Mark (X) the ® POTWprovide name(s) below: 

CBI 

0 Navigable ~ Other - Speciffi provide NPDES # CBI 

destination(s) of waterwav 

releases to water. 

Maik (X) this box if you attach a continuation shect. _ 
1>atutn : (t-5 .(12 .()9 

Seitr I vori Ll 

7'hc P1VIn _tic alcohol ethoxvlate nrecursor~ 

1'1VIN substance was estimated based on this data. 

Analytical Report 

Sample code sponsor : 

' Sample description : 

Sample received on : Oc) .0~ .?007 

! Date of issue : i '?9 .05?007 

~, Sponsor: 

'I Sampled b~ - -

I Responsible for the study: i 

- i 

i Record/project number 1 0T5/9-0507 

~ (internal) : 

Determination of the Avera~e Molecular ~~'eight 

and the Molecular Wei~ht Distribution of 

1 . Summary 

T1tP a-eragP t~~~~P~>>lat~a7ei`ht and the molecular weight distribution of the substance 

w s determined with gel permeationcluomato(Traphy (GPC). The 

?~agel in the caii~iatton was done with polyethylene glycol. standards from 1\~achere~~ L 

range from 20000 - 600 R/mol. . 

Sample-No . Sample description 

(gintol) ; (g mNoI) 

M < ;00 

(C_,/mol) 

M<1000 

~- (g/mol) I 

ESDB 01636? 

2. Description of the Test Method 

2.1 Principle : 

The molecular weight distribution and the average molecular weights Nil. Mw) are 

determined with GPC . GPC is a special type of liquid chromatography in which the 

sample is separated according to the hydrodynamic volumes of the individual constituents . 

Dclcction is effected by e.g. refi~active index or MI-absorption and yields a simplc 

di~t1ibutioil curvc . 

To attribute actu_!I molecular \vciQht values to tl~c ::tirve, it is necessary to calibr~,itt: the 

colutnu by passing down polynlcrs of knovvrn tnolccLilar weight- c.g . various polyethylene 

(flycol standards . 
p. 31 

Datum : (l^,-, .(12 .0() 

Scitc --' von 4 

2 .2 Experimental details 

1 Columns i .lordi Gel D\'B 10- A ?i0 mm x 10 mm ID 

{ Jordi Gel D\jB 10' A 250 mm x 10 mm ID 

I~ ,lordi Gel D \'B 10; A ?i0 mm a 10 mm ID 

~ (connected in series) 

i ElueIlt j rretrahydrofLlrall (THF) I 

( Temperature I Room temperature 

i Flow rate ; 1 .0 mL!min . 

i Pump BISCHOFF Model 2250 i 

j Iniector GILSON autoiniector Model -",4 

i Iniection volume ; 20 ~LL - I 

; Detector 

i Sample concentration 

i Evaluation 

! BISCHOFF differential refractometer Model 8120 

I 180 rrial10nIL 

j Cirrus GPC-Software ~~ersion `? .0 

~, (Polymer Laboratories Ltd .) 

2.3 Calibration curve of the PEG standards 

Clrrus GPC Calibratlon R-.- 

Gan~ratad b --p 

Uqnnoslag 2n 

vrus WorkOqqka~07-Lrpqpnli-6nala0~-Lrpqpnr:-Mar plw 

Ma . 2D07 09 OO 

GallbratrqnO . .2007 O a:03 

ra ~ c rr w Slandard Gurvc Frl Usod 3 

Galrnr ~ionGUrveNiB6r-. _~ 326x^i-OOaiitY ~- 0000<7sir ~ 

I-Upn Lrrmrl MVa 2000C~ 4 

mms 

a .07000 

RCF It 0000 

sr ai Sum OI Squams OOODOO 

Goe11 u0 rmrna r OODDOO 

Lrnoar Gorralabqn Cocn ,-O 9bB216 

600 

Cr .rn.. GvG vcr,ron 7 U 

w r VY RT 3t .<3 mrns 

FRMLN'rmc 

1=1qw Markcr R7ud_.bi mms 

orrac u Sum OI 5 u r s 

SlanqarC Y Error Esbmata i aINF00 

rn Usc RErr r 
Datum : 01.02 .09 

3 . Chrolnatobrarn 

3 .l . 

z-`-

"tE2az~--

2 ;- 

ESDB 01"c3E2 

,e_ 

Distribution Plots 

SCtlr ~ votl 4 

t o: 

sc 

- ec 

-t . 

7c 

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I)atliIll : lt~ .U .: .09 

Senc 4 voti 4 
DR U NOACK LABORATORY FOR APPLIED BIOLOGY 

(signature] 

6;"?6190 

Report : 

Test of Acute Toxicit~~ to Fish Log No. 1288 

OECD 2(13 I 

Tested substance: 

Table of Contents Page 

Abstract 1 

Test report 2-5 

Graph of mortality ? 

Test protocol of main test 6-9 

Test concentrations, measurements of water parameters in the test preparations : 

oxygen. pH, temp ., hardness, stability figures 

Solubilitti trials 10 

Testing plan 1 1 - 1? 

Certification by quality assurance staff 13 

Certificate of Analysis (from client) 14 
p. 35 

DR U NOACK LABORATORY FOR APPLIED BIOLOGY 

Report : 

Test of Acute Toxicity to Fish Loo No. 1288 

I OECD 203 

Page I of 14 

Abstract 

Tested substance 

Client L 

Lo(-, No. :1288 

Main test on :05/14/1990 

Test results 

LC51, (96 h) 1 .6 [mg/1] 

Lowest tested concentration of the tested substance 

in a test preparation with 100% mortalih~ ? .5 [ma/1] 

Hi2hest tested concentration of the tested substance 

in a test preparation without mortality : 1 .0 [MR/1] 
DR U . NOACK LABORATORY FOR APPLIED BIOLOGY 

p. 36 

- 

Report: 

Test of' Acutc Toxicity to Fish I Lov , No. 1288 

OECD 203 

PxRe 2 of' 14 

Test Report for the Substanc~ 

Code unknown 

Batch -L.1 E02-24a()9 and E012-24576 

Client 

Laboratory Log No. 12£F 

1 . Test system : Brachv_ danio rerio (zebra danio) 

1 .1 Breeder : -- 

1 .2 Source : Stumpe Zoo Supply. Hildesheim 

1 .3 Nutrition : Dry food (Manufactured by Tetra) 

1 .4 Fish upkeep : DR. U. NOACK LABORATORY . fish breeding area (KG) 

2. Number of animals in the test : 10 fish per test concentration 

2.1 Testing vessel : 16-liter-volume tanks 

2.2 Volume of test medium : 5 liters at rest 

?.3 Number of animals per test receptacle : 10 

2 .4 Test solutions for the main test : Test concentration from stock preparation 

with tap water 

Test preparations : 0.5 ; 1 : 2.5 : 5: 10 [mg/1] 

2 .4 Liahting : Diffuse liaht 

approx . 10 [pE/m- a s] with alternation even, 12 hours 

'? .6 Testing plan : see pa. 12 

3 . Reference substance : no reference substance 

3 .1 Reference toxicity : ---- 

4. Testing duration 

4.1 Solubilitv trials : 04/30/1990 

4.2 Date of preliminary test . 04/30/1990 

4.3 Beginning of main test . 05/14/1990 

4.4 Testing, duration (main test) : 96 hours 

4.5 Homo;eneity of the test preparation and test concentration by visual evaluation : homogenous 

5 . Concentration of oxygen in solution, pH and temperature in the test preparations : see p`. 7 

6 . Equipment 

6 .1 Equipment for water parameters 

Oxygen : NArTW, Oxi 191 

pH : pH 240 . manufactured by Corning Glass 

Temperature : WTVd, Oxi 191 

Conductivity : VdTW. LF 191 

6 .2 Analysis equipment: CADAS 100 photometer, manufactured by Dr. Lange GmbH 

6 .3 Spectrophotometric detection of the tested substance (UV/VIS . 200-700 nm) from the test 

preparations was not possible . 
p. 37 

DR U NOACK LABORATORY FOR APPLIED BIOLOGY 

Report : 

Test of Acute Toaicit`~ to Fish Log No. 1288 

OECD 203 

, Page 3 of 14 

Pa«e ? of test report 

7. TESTED SUBSTANCE 

General remarks on the tested substance 

Name 

Appearance : white paste 

Melting point unknown 

Boiling point unknown 

Water solubility soluble (slightly cloudy solution) 

Distribution coefficient n-octanol/water: log it, p = unknown 

8 . Test result : 

i; .l Cumulative mortality rate in the individual test preparations : 

Concentration 

[mc/1] 

Cumulative mortality [%] ', 

bv testin- duration in hours 

24 48 72 i 96 ' 

10 .0 100 100 100 100 ' 

5 .0 100 100 100 100 

2.5 j 100 100 ~ 100 100 

1 .0 0 0 I 0 0 

0.5 0 0 0 0 ' 

control 0 0 0 0 ' 

8 .2 Graph of mortalih~ rate : see p~. 5 
p. 38 

DR U. NOACK LABORATORY FOR APPLIED BIOLOGY 

Report : 

Test of Acute ToxicitY to Fish Log No. 1288 

OECD 203 

1 Page 4 of 14 

Pag e 3 of test report 

9 .3 LCS values for each observation time 

Test duration lt LC,(, m~/I P 9S%~ 

24 1 .6 1 .0 2 .5 

48 1 .6 1 .() 2 .> I 

72 1 .6 1 .0 2 . 

96 1 .6 1 .0 --- 2 .5 

8 .4 The lowest concentration of the tested substance in the test solution . in which the substance caused 

100% rnortalit-v : ?.> [mg/1] 

8.5 The highest concentration of the tested substance in the test solution . in which the substance caused 

zero mortality 1 .0 [ma/1] 

9. Statistical procedure: 

after Speatman and harber in : L. Sachs, Angewandte Statistik [Applied Statistics], pa. 117-18 1 ; 

Springer Verlag, publishers . 1984 

The qualit~~ criteria according to OECD 303 were fulfilled. 

Hildesheim . 6/13.190 

tSTAv1P] DR. U NOACK LABORATORY [signature] 

FOR APPLIED BIOLOGY 

Technology Center Dr. U . Noack 

Richthotenstratie 29 (Graduate Biologist) 

D-3200 Hildesheim Director of the Testing Facility Tel . 05121176 03 53 and 5 78 99 

Far 05121/76 03 44, Telex 51 21 926 tzhd 
DR. U NOACK LABORATORY FOR APPLIED BIOLOGY 

I Test of Acute Toxicity to Fish 

~ OECD 203 

Page 4 of test re port 

Log No . 1288 

Page 5 of 14 

Wahrscheirnlichkeitsnetz = probability grid 

HaufiL,keitssummen in °/t, der Gesarntzahl = Cumulative frequency in °rb of total 

[illegible term in right margin] 

[graph title] Mortality [%] as a function of concentration [mg/1] 

DR .'U .NOACF :-LABORATORIUM FUR ANGEWANDTE BIOLOGIE 

Outer Toxlzltdtstest an Flsc(1en Journal-Nr . : tzee 

(ICCp 203 

Sel1e 4 zllm PrUfbericUt Selte 5 Yon 14 

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Mortalltat [Z] In Abllbnglgkelt Yon der Konzentration [mg/~ 

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00 
DR U . NOACK LABORATORY FOR APPLIED BIOLOGY 

Report : 

Test of' Acute Toxicity to Fish 

OECD 203 

j Log No. 1288 

i Page 6 of] 4 

Test protocol of main test 

Tested substance 

Name : 

Appearance : 

Melting point : 

Boiling point : 

Water solubilit~~ : 

Distribution coefficient : 

2 . Preparations 

2 .1 Test system 

Name 

Source 

Len(-)rth 

Weight 

white paste 

unknown 

unknown 

soluble (slightly cloudy solution) 

n-octanol/water: lob,(, p = unknown 

Brachvdanio rerio (zebra danio) 

Stumpe Zoo Supply. Hildesheim 

approx. ?.8 cm 

approx. 0.24 ~ 

Test conditions 

Water temperature 

Disinfection conducted 

Acclimatization conducted 

Duration of test 

Volume of test tanks 

Test volume 

Number of fish per fish tank 

Tested concentrations 

Number of fish per concentration 

210C 

cleaninQ and ?4-hour rinsin2 of the tanks 

per OECD guideline 

96 hours 

16 liters 

5 liters (at rest) 

10 

0 .5 : 1 : 2.5 ; 5; 10 [m g/]] 

10 

3 . Analysis 

Detection of test chemicals in water: Spectrophotometric detection of the tested substance (UV/V1S, 

200-700 run) from the test preparations was not possible . 
p. 41 

DR U . NOACK LABORATORY FOR APPLIED BIOLOGY 

Report : 

Test of Acutc Toxicity to Fish Loc No . 1288 

OECD 203 

Page 7 of 14 

Test protocol of main test 

4 .2 Observations in the fish tank: 

Tank No Colic. Effect Time Id . 

I mg/lI ~ 1 2 3 4 

1 10 .0 letharn 

death 10/10 10/10 10'10 10/10 

2 5 .0 lethargy I 

death 101110 10"10 10/ 10 10/10 

3 ?.5 lethargy 

death 10!10 i 10/10 10!10 10/10 

4 1 .0 ~ NAD 

death 10/10 10/l0 10'10 10/10 

; 0.5 NAD 

_ 

death 10/10 10!10 10/10 10/10 

(, control NAD ' 

death 10/10 10'10 10,10 10/10 

NAD - no abnonnalit\, detected 
p. 42 

DR . U. NOACK LABORATORY FOR APPLIED BIOLOGY 

Report : 

Test of Acute Toxicity to Fish 

OECD 203 

Test protocol of main test 

4 . Measurement of water parameters : 

Log No . 1288 

Page 8 of 14 

Sample collection 

Date and time 

Beginning of main test (0 h) 

OS/14/1990, 2:30 In. 

Tank No. 

1 2 3 4 5 6 

Oxygen content mg/11 b .9 8 .8 8 .8 j 8 .9 8 .9 8.3 

8.05 8 .04 7.99 7 .94 7 .82 7 .87 

Temperature (°Cl 19 I 19 ~ 19 ~ 19 ~ 19 ~ 19 

Sample collection 

Date and time 

Day 1 of main test (24 h) I 

OS/15/1990. 1 :30 .m . 

Tank No. 1 "' 3 4 5 6 

Oxygen content mQ/11 8 .6 8 .5 8.6 8 .5 8.6 8 .3 

5.08 8.04 8 .00 8 .12 ~ 8 .08 7.98 

Temperature 1 'CJ 21 21 

Sample collection 

Date and time 

Day 1 of main test (48 h) 

05/16119 0 . 12 :30 .m . 

Tank No. 

1 2 I 3 4 5 I 6 

Ox~~aen content ma/1 I --- --- I --- 8.4 8 .3 8 .6 

--- --- --- 8 .03 7 .96 7.94 

Temperature [°C1 _ --- ~ --- I --- 2' "- 

Remarks: At the be- 

-` 

of the test . the test temperature was marginally lower than the target 

range. The course of the test, however, is plausible and was evaluated. 
p. 43 

DR U NOACK LABORATORY FOR APPLIED BIOLOGY 

Report: 

Test of' Acute ToaicitY to Fish Lob No. 1288 

OECD 203 I 

~ Page 9 of 14 

Test protocol of the maiu test 

4. Measurement of water parameters: 

Sample collection 

Date and time 

Dav 3 of main test (7? h) 

0-5/17/1990 . 11 :30 a.m. 

Tank Na. 1 2 3 4 5 I 6 I 

Oxygen content mRll - -- -- 

- 

--- 8 .4 9.3 8 .7 

H 

_ 

--- - --- --- 8.11 8 .05 7 .94 

LTemperatu re J°Cj ?1 ~ ?1 21 - 

Sample collection 

Date and time 

Day 4 of main test (96 h) 

05!1 S/1990. 10:00 um. 

Tank No. ~ _ 1 _ 2 3 4 5 6 

~ Oxygen content 1 ma/1) --- --- 

_ 

s .6 s .6 s .3 ' 

pH ~ --- ~ --- --- 8 .16 ~ 8 .06 ~ 8 .02 

Temperature (°C) --- --- --- ?2 ?2 2? 
p. 44 

DR U NOACK LABORATORY FOR APPLIED BIOLOGY 

Report : 

Test of Acute Toxicity to Fish Loo No. 12$8 

OECD 203 

PaRe 10 of 14 

Solubility trials for the Test of Acute Toxicity to Fish OECD 203 

Tested substance 

Laboratory Lo(-, No . 1''8b 

Trials on I ~ 04/30,11990 

Attributes 

Solvent K. solubilizer 

Dilution water : soluble 

Ethanol DABS Merck : --

Acetone puriss . Fluka : --

DMF puriss . Fluka : --

DMSO puriss . Fluka : --

Tween 80 puriss . Fluka . -- 

Remarks: -- 
DR U NOACK LABORATORY FOR APPLIED BIOLOGY 

Report : 

Test of Acute Toxicity to Fist, 

OECD 203 

Test plan 

I-- 

Loo No. 1288 

Page l 1 of 14 

1 . Tested substance 1 _ 

1 .1 Code : unknown 

1 .2 Batch 1 :l E02-24499 and E012-24576 

1 .3 Solubilitv : soluble (slightly cloudy solution) 

1 .4 Volatility : unknown 

1 .5 Manufacturer : see client 

?. Client : 

2.1 Address 

3 . Testing facility : DR. U. NOACK LABORATORY FOR APPLIED BIOLOGY 

3 .1 Administrator : U. Ballnus 

3 .2 Testing methods and Guidelines : OECD 203, GLP auidelines 

3 .3 Testing system : Brachydanio rerio (zebra danio) 

3 .3 .1 Source : Stumpe Zoo Supply (Hildesheim) 

3 .4 Test concentrations : The test concentrations were prepared by diluting the stock preparation. 

The concentrations were determined accordina to the results of the preliminary trials or other trials . 

;,5 Reference substance : none 

3 .6 Use of organic solvent : --

3.6 .1 Stock solution : --

3.6? Concentration : -- 

3.7 Concentration range of the tested substance [mg/fl : 0 .5-10 

3 .7 .1 Stock solution : 100 [mg/1] 

3 .7 .2 Special sample pre-treatment: -- 

3.8 Frequency and duration of application: At rest, for a period of 96 hours with ventilation 

3.9 Type and frequency of measurements conducted and observations : 

- Analvsis of test water: daily (pH, oxygen content, temperature) . 

- Oxygen saturation should not fall below 60% 

- Temperature should be 23 --'2°C 

3 .10 Preliminary trials to limit the concentration range : 

- Solubility trials : soluble (tap water) 

- Preliminan, test : 4 concentrations (4 fish each) for a period of 48 hours 

0 .1-100 [mg/1] ; factor 1/l0 

- Concentration range for the main test : 0 .5-10 [ma/1] 
p. 46 

DR. U NOACY. LABORATORY FOR APPLIED BIOLOGY 

Report: 

Test of Acute Toaicitv tu Fish Loo No. 1288 

OECD 203 

Page 12 of'14 

Testing plan 

3 .11 Description of the test progress 

- Standard operating procedure (SOP OECD 203) 

- Intended timefratne for the test : 05/14,11990 - 05/18/1990 

-Test concentrations : 0.5 : ) : ?.5 : 5: 10 [mg/1] 

Hildesheim, 05/2!9D [siRnature] 

(Date) (Administrator) 

Hildesheim, 05,'2/90 [signature] 

(Date) (Direetor of Testim-, Facility) 
DR U NOACK LABORATORY FOR APPLIED BIOLOGY 

Report: 

Test of Acute Toxicity to Fish 

OECD 203 

Certification bN Quality Assurance Staff 

Log No. 1288 

ace 13 of-l4 

Inspection for biological test Acute Toxicity in Fish . OECD 203 

LoL~ No . : 1288 

Final inspection on : 06/08/1990 

REMARKS / OBJECTIONS 

1 . Equipment calibration 

Inspection on 05115/1990 : No objections 

2 . Substance test procedure 

Inspection on OS/14/1990 : No objections 

3 . Reference substance test : ---- 

Objections and notice sent 

to the Test Director on : -noneHildesheim . 06/OS11990 

[signature] 

Quality Assurance Staff 
To: 

Dr. U Noack Laboratory 

for Applied Biology 

Richthofenstrasse 29 

3200 Hildesheim 

Dear Sirs : 

We offer the enclosed sample to determine its acute toxicity in fish by: 

-- Requirements of EC guideline C .1 . Acute Toxicity to Fish, Guideline 84/449/EEC : Guidelines 

of the Commission of April 25, 1984 on the sixth adaptation of Guideline 67/548/EEC of the 

Council on Equalization of Legal and Administrative Regulations for the classification, 

packaging and identification of hazardous materials in advanced technology, and the 

-- OECD Guideline for testing of chemicals, 203 Fish, Acute Toxicity Test, Adopted : April 4, 

1984 (48/96 hours) 

in accordance with GLP principl~ 

Trade name of the sample: 

Composition : 

Batch identification : 1 :1 E02-24499 and E012-24576 

Physical data: 

Consistency : paste Density: approx. 0 .95 glcm3 (50°C) 

Flash point: 190°C pH (10 g/I) : 6-7 

Solubility in water: soluble (slightly cloudy) 

Solubility in organic solvents : ethanol, isopropanol 

Stability : over 2 years under normal conditions 

We request that you send us the test report in triplicate along with your invoice . 

Sincerely, 

Frankfurt . 2/12/90 

(Place/Date) (Telephone) 

[stamps' 

[signature] 

(Signature and Name stamp) 

Li 

I .-!\ 
p. 49 

Department : Analytical Laboratory 

Author : Eco-Chemistry/Analysis E 

Dr. H.-'A/ . Biickin,,,' . D. Pleschke 

Summary report of the testing of biodegradability 

according to modified OECD screening test 301 E 

l . Information on the test 

The test is conducted accordinb to guideline 84/449 EEC (Part C). EC Gazette L?5 1/160 dated 9/19/84 

and also fulfills the requirements of the OECD Guidelines for testing of chemicals. Guideline 301 E. 

The purpose of this procedure is to determine the biodegradability of soluble, non-volatile organic 

compounds in an aerobic. aqueous medium with an initial concentration of 5-40 ma DOC/l 

(=dissolved organic carbon) . 

Test No. : 568(2)-68-V. HX 470-2 

Client : Dr. Jurges/Dr. Crass, ETH-1. D 56? 

Test start: 04,128!93 End of test : 05J26/93 

Information on the tested substance 

Description 

Type of test sample 

Composition 

Formula 

3 . Results 

: non-ionic tenside 

: 100% W S =tested substance 

: see graph in the appendix 

The biodegradability of the testing substance is specified and represented graphically in the appendix 

(Table 1) . 

Evaluation according to DIN 52 900 (DIN safety data sheet) 

Degree of Conclusion Testing method Analysis method 

degradability 

(28 d) 

96% easily degradable tnodified OECD DOC determination 

screeninE test 301 E 
Department: AnalNnical Laboratory Date : 06/09/93/hs 

Author : Eco-Chemistrv/Analysis E Report No : 117/93 (B) 

Dr. H.-W . Buckinb. D. Pleschke Page: '? (4) 

4 . Tested substance solution 

Mass (as is) : 0 .017 a/I 

pH value after preparation 7? 

Solution preparation agitated for : 0 .5 hours 

Temperature during agitation : 23°C 

pH value after the specified agitation period : 7 .? 

The tested substance was subsequently dissolved in water, forming a clear solution . 

. Determination of the composite parameter 

DOC was determined according to DIN 38 409 (Part 3) and SOP 285-X/90 . 

6 . Test preparation 

6 .1 Inoculum 

Applied amount : Sludge frotn the activation tank of Frankfurt's Niederrad 

wastewater treatment plant 

corresponding to : Approx. 106 colony-forming units per liter 

pre-treatment : Ventilated I day without adding anv nutrient solution 

adaptation : Adapted neither to the tested substance nor the reference substance 

in the laboratory 

6 .2 Testing conditions 

Incubation was conducted at 20 ±2°C. The composition of the test preparation corresponded to the 

requirements in OECD 301 E. The activit~~ of the inoculum was verified with the reference substance 

sodium benzoate and a preparation without any additional tested substance was examined as a control. 

Rounded glass beakers with a capacity of I liter were used as preparation vessels. The beakers were 

covered with aluminum foil . Oxygen was not supplied . Biodearadation occurred in the dark in a 

mechanical shaker . 

[signature] 

(Dr . H.-W. Buckin~) 

Enclosure 
Department : Analytical Laborator~~ Date : 06/09t93/hs 

Author: Eco-Ehemistry/AnalysisE F',eportNo : 117!93 (B) 

Dr. H.-VV . Bucl:ing . D. Pleschke PaLe: ', (4) 

Appendix to the report_ Test No . 568(?)-58-V. 1-IX 470-2 

Table 1 : Total dm- -adation accordin~~ to modified OED screening test 301 E 

Tested substance 

t 

Timc 

(d) 

Reuctivc 

pre paration 

Reference substane Tested substance 

DOC 

m c/I 

llOC 

m /I 

Uegrsidntion 

(%) 

DOC 

ntall 

' Decrease in DOC' 

J (°ro) 

0 0.6/0 .6 20 .0 _ 8 .6 I _ 

7 0.3/0.3 1 .9 91 .0 7 .7 7.~ ! 

14 0.3/0.3 2.0 912 2 .1 77 .5 

21 0.3/0 .> 19~ 92 .> 1 .5 87 .5 

27 0.3/0 .3 1 .4 94 .3 1 .2i 89 .1 

, 2b ~ 0 .5/0 .6 ~ 1 .4 ~ 9~ .6 ~ 0 .8~ I -- 96.2 - - J 

*Sodium benzoate 
Department : Analytical Laboratory Date: 06/09/93/hs 

Author : Eco-Chemistry/Anahysis E Report No. : l M93 (B) 

Dr. H.-W. Bucking. D. Pleschke Page : 4 (4) 

il., 

Graph 

Total decay according to modified OED screening test 301 E (1981) - 

Control 

sodium benzoate 

k/J 

50 

Ui/t 

40 

20 

% reduction of DOC (dissolved organic carbon) 

r-- 

" 

C 7 

~.. . . . . . . . . . .~ 

1 

100 

n 

5 30 1.5 20 2,5 30 

Duration of test in days 

Exhibit 3 
p. 53 

7b , ~6~.~AAC 

Test report : composite parameter 

Report number: (Stock No. - Trial ID) 

v-8c)-760-S 

Sample Specification: see page 1 

Paoe : ? (4) 

Specification of Composite Parameter : 

Measured Values Calculated . 

SITOX 

No. 

Parameter Test standard in the 

stock solution : (1) 

per g substance. 

calculation: (2) 

theoretical 

values : (3) 

451 DOC DN 3s 409 Part 3 3250 mQC/I 650 mgCl_~ mg C /,g 

452 COD EEC s4/44> and 

DIN 3b 409 Part 41 

11373 mg0,/l 22,'5 m-0,/1 mg0'/1 

-- -- -- - - - - -- -- L --- - - - - ------ 

Comments : 

(1) For product testing: Stock solution according to the specifications on page 1 of this report 

For Wastewater testing: Values projected based on the undiluted wastewater sample 

(?) Methods of determinim the values per gram are checked below 

[X] For solutions and stable dispersions with a known weigh-in value this figure is calculated per gram 

from the measured values per liter of stock solution . 

[] 

[] 

[ 

For pure substances that are scarce]), soluble. an indirect method is used to determine the COD, using 

the measured value of COD and the measured value of DOC of the saturated solution with the aid of 

the calculated DOC/g figure . The maanitude of DOC/g is not capable of being determined by 

measurement. 

For partially dissolved mixed preparations, no values per gratn can be calculated, because the 

concentrations of the individual components can no longer be established after filtration . The COD of 

such products are specified with direct mass (approx. 1-2 mg) in the reaction vessel . The magnitude of 

DOC/g is not capable of being determined by measurement. 

For the purpose of COD determination, filtered dispersions are treated as nearly-insoluble substances, 

if the calculation of DOC/g is possible, otherwise as partially-dissolved mixed preparations . The 

method that was chosen is designated by checking a box next to the selected method above . (The 

separation of coarser particles or droplets by filtration is necessary in order to determine COD and to 

conduct further testing, if need be). 

(3) The DOC and a theoretical COD value for 100% oxidabiGty can be reckoned from the molecularg 

formula (if known). They are determined only if they are needed as a launching pad for other 

calculations or for the analysis of specific ecological trials ; also whenever there is doubt concerning 

significant deviations from the measured, values in crams. r~ 

Comment: For multiple measurements of composite parameters when setting up various partial tests, the 

values may vary due to the range of errors for each analysis method. Significant deviations are possible 

when making saturated or other filtered solutions according to the standard methods specified on page 1 . 

[signature] 
p. 54 

'f esl report . Ialii~-\\%cllem,'l esi 

hepor( number: (Moll No. -'I rial I I)) 

700-A 

I ainplc~ ~ptcifiGation: seu paL-,,: I 

1'w_,c : ? (4 h 

( 

1 

Lxamination oi biodepradabilit, b~ rrneturs of tlrr lahr~-V~~cllens "f es! 

I General specifications Sitox No . 4~4 

The test is conducted accordinL, to Luidelmcs 8&,'302/EEC War! C1 . EC Gazette L133 . 5!3)0'1 9M,' and also 

rneets the requirements of DIN 3& 4 .2 Part 2S (static test) as ~nell as OECD Guidc)rnes for testin~~ of 

chemicals . Guideline 302 B . 

The Zahn-Wellens test is used to test whether a substance is biode~~radable in principle The result does ]lot 

enable us to reach am conclusion reLardin~~ -sliLht biodearadability .'" Data on adsorption and elimination 

app1y exclusivel~~ to the activated sludac that was used . These values are not transferable to slud`re frorn 

other sources. 

'_' . Test preparation 

/-Activated sludge: 10 :c!1 centrifuged liquid sludge ~ 870 m~~/ 1~/1 dry weight 

/ ~ ~J0 :70 bioloQical wastewater treatment plant 

t est sample : I-Ij mi rroM Me stock solutiorn (page I ) in a total volume o;°? liters . 

Calculated initial concentration : DOC = 300 tnL C/l : COD = ,' m<_ O,/l 

3 . Results : Table and g~aph on the following page 

4 . Evaluation of biodegradability (applicable statement is checked) 

In the Zahn-Vdellens test under Quideline 88,'30?/EEC Part C and OECD 30? B. the test sample is 

[?~] Easily degradable : range > 70°/0 (specifi': >70 . >75. >80 or >90°/0) 

[X] in < 7 davs ; [ ] u> > 7 to 28 days : >90~6 in 2_5 dcns 

[ ] Degradable in pruaciple: range >20°/o to 70°/0. Decay is slow but steady . 

[ ] >_°/o degradable if the test's duration is extended (>38 d), in - days 

[ ] Partially degradable : ransie >30°rb to 70°/0. Residue remained after partial decay. 

[ ] Not deQradable . (A reduction of the measured values between 0°/o and 30% is not [considered) 

si anif cant . ) 

Comment: For the ecological evaluation of chemicals. only this information should be used fo : evaluation . 

and not individual values from the folloNvinsi table . because the reproducibilit\~ of biolo-2ical test procedures 

does not (uarantee precise percentage values, but can only establish a range of values . 

Adsorption in activated sludge (after 3 hours) 1?°~0 

As a result of higher adsorption [ ) the statement on biodeyradabilitv is ne(ativelv influenced . 

[ ] no statement on biodep 'adabilit-y is possible . r 

., Projected efimination in industrial wastewater treatmertf nlantr annrnx 7>°.0 

\( 

Biodegradable as per WMG [signature) 

(l~Fashir?g and CIeaTSel's ActJ 
"f e,st No \ -fi(J-U7o(,-l ; 1'rcharauo!! No . I 1'aLC ~ t-1 I 

WIC 04 IN /()(I 

Lletet-minativn ot hmdes!'radahilit\ h\ trtc :ait : . o tlic ZtthtI-\t,%cllens 'I est 

Tes; suhstancc : 

Appiication concenu-atiot? 1 ~> ~ m~/l DC>C 

nieasured as dissolved c~rLantca)l\ bonded car6on (=DCtCt 

Ivicawrenent unic 'Iota!1 Liiudeeradtuuot, Avera_~c~t I :au2c 

aitef stt)rww ncunt euminauorn Xvrthoui aclsornuoe et!n . de ;eradauoI : 

(lttiue!c;deulated accord!n_~ W (treua avCra~cd~ 

u! . iomiuliu ~ 

(1'S . , 

t dN~ .~b`io 77'in 

-dflY: c~ea =1 ;,, 

- dav~ ti0°,, 7-7u,(, c! -1q1- 701-

^ del" 7"°r,. 74'i, i d 78`i, 7611,. 

y dav~ ~ b()c5,~ 77% 

,- davs 84',,. 829< 

14 da1,s 92 9,, v1 °, 

lu da1~s y 0`/l i SR°, 

19 darc l;b , Sb~o 

31 davs b9~<, 88111( . 

_3 dn'z 9 -16 i 

_-daVs ~?°a 9_io 

AU O":U & davs 1 no y~ i< : c, '1~ " o c q- i 

[~--aais : Biodeg-adation . 

elimination in °/'o 

10M, 

tio% , 

o°,- 

1G 3C 

[x-axis :] Time sample was taken. counted in days from 

the beRinrninR ofthe trial 

Biodem-adation in the Zalui-VJellens Tesi 

e Biodearadation 

U~01o 

Symbols [sic] : accordim: to analysis of calculated p°rcentage values 

Lines: trend. in par ; computationalk smoothed 
p. 56 

DR U NOACY. LABORATORY FOR APPLIED BIOLOGY 

[handwritten :] OIQ7901?1 

[signature] 

10/1/91 

Test of Bacterial Toxicity 

in Activated Sludge - Respiration Inhibition Test 

by 

r-` 

according to OECD Guideline No. 209 

for Testing Chemicals 

in Activated Sludge - Respiration Inhibition Test 

(04/04/1984) 

Client : 

Lou No. 

Study Director 

Administrator 

Quality Assurance Unit 

Director of the Testing Facility 

-J 

: 2080 

: M. Noack (Biol.) 

: 1 . Rautenberg 

. U. Wolf (Dipl.-Bio) .) 

: Dr. U. Noack (Dipl.-Biol .) 

DR. U. NOACK LABORATORY 

FOR APPLIED BIOLOGY 

Rich thofenstra Be 29 

D-3200 Hildesheim 

Germany 

Date: August 26, 1991 
p. 57 

OR. U NOACK LABORATORY FOR APPLIED BIOLOGY 

Report: Activated Sludge - 

Respiration Inhibition Test accordin~ to Lo~; No. 2080 

OECD Guideline 209 

Test of Bacterial Toxicitr- Page 1 of 12 

Table of Contents 

Number of pages Page C~ 

Certificates of authenticitv_ and 

quality assurance 4 

Abstract 1 6 

Methods 2 7 

Results J 11 9 

Graph of the ratio 

of concentration to efficac~~ l 1? 

Appendices 

Testing plan ; 

Client's product data sheet 1 

Certificate 1 
p. 58 

DR U NOACK LABORATORY FOR APPLIED BIOLOGY 

Report: Activated Sludhe - 

Respiration Inhibition Test according to Log No. 2080 

OECD Guideline 209 

Test of Bacterial Toxicitv I'age 2 of 12 

Statement by the Entire Staff' 

We hereby cel1ify the authenticity of this test and assure tlhat, to the best of our knowledue. all data have 

been accurately recorded and are correct . 

The test was conducted according to the procedures described herein . 

Study Director : Martina Noack [handwritten :] b/13/91 [signature] 

(Biologist) 

Dr . U. NOACK LABORATORY 

FOR APPLIED BIOLOGY 

Richthofenstrafie 29 

D-3200 Hildesheim 

Tel. : 05121/760353 

Fax: 05121/55714 

Administrator : Iris Rautenberg [handwritten :] S/14/91 [signature] 

Address same as above 

Quality Assurance 

Staff : Ursula Wolf [handwritten] 8,'14/91 by order [signature] 

(Graduate Biologist) C~ 

Address same as above 

Approval of the Test 

Report by the Director 

of the Testing Facility : Dr. Udo Noack [handwritten] 8/26/9l [signature] 

(Graduate Biologist) 

Address same as above 

[seal] 

State-accredited testin2 facility 

DR. U. NOACK LABORATORY 

FOR APPLIED BIOLOGY 

accorduig to § 19B ChemG [Chemicals Act] with GLP 

certification 
DR U . NOACK LABORATORY FOR APPLIED BIOLOGY 

Report: Activated Sludge - 

Respiration Inhibition Test according to ~ Log No . 2080 

OECD Guideline 209 

Test of Bacterial Toxicitv 

Lob No . 

Substance tested 

Page 3 of 12 

Statement by the Quality Assurance Staff 

Title : Test of Bacterial Toxicity in Activated Sludge 

Respiration Inhibition Test 

under OECD 309 

An analysis certificate of the substance tested is not available. Please see the enclosed certificate . 

Inspections conducted: 

Inspection of Inspection date 

Test procedure 

(evaluation of methods) 

Equipment calibration 

Test results 

Final report 

Objections and messages 

to the Study Director : - none - 

6125/1991 

06L24,11991 

07/10/1991 

08/141991 

Hildesheim, August 14, 1991 by order [signature] 

[stamp] DIPL.-BIOL . U. WOLF 

Qualit>~ Assurance 

DR. U. NOACK LABORATORY 

FOR APPLIED BIOLOGY 
DR U. NOACK LABORATORY FOR APPLIED BIOLOGY 

Report: Activated Sludge - 

Respiration Inhibition Test according to 

OECD Guideline 209 

Test of Bacterial Toxicity 

Loci No . 2080 

Page 4 of' 12 

Good LaboratorY Practice 

STATEMENT BY THE STUDY DIRECTOR 

Lou No . : 2080 

Substance tested 

Study Director : ~--Martina Noack 

- (Biologist) 

Dr. U. NOACh LABORATORY 

FOR APPLIED BIOLOGY 

Richthofenstraf3e 29 

D-3200 Hildesheim 

Tel. : 05121/760353 

Title : Test of Bacterial Toaicit~- in Activated Sludge 

Respiration Inhibition Test 

under OECD 209 

To the best of my knowledge. this test has been conducted in compliance with the guidelines for good 

laboratory practice (BGBI [German Federal Law Gazette] of 03'2211990 . No. 13, addendum I, 

page 539 et seq.) 

[handwritten] 8/13/91 [signature] 

Date Martuia Noack 

(Stud), Director) 
DR. U NOACK LABORATORY FOR APPLIED BIOLOGY 

Report : Activated Sludge - 

Respiration Inhibition Test accordin(i to i 

-I 

Log No. 2080 

OECD Guideline 209 

Test of Bacterial Toxicity 

Page -5 of 12 

Confidentiality Agreement 

e . the undersismed, hereby certi~v that all information related to this report . including that supplied b~~ 

,ill be treated as confidential . 

This also inclu es all data recorded in the course of conductiivt; this study. 

All reports and results related to this study are the propert~ J 

The LABORATORY OF DR. NOACK may not disclose sa~d data to third parties without express 

agreement in writing. 

Hildesheim . Ob'/13/91 [signature] 

(Date) Martina Noack 

(Study Director) 

p. 61 

Hildesheim, OS,L?6/91 [signature] 

(Date) Dr. Udo Noack 

(Director of Testina Facility) 
DR U NOACY LABORATORY FOR APPLIED BIOLOGY 

Report : Activated Sludge - ' 

Respiration Inhibition Test according to 

OECD Guideline 209 

Test of Bacterial Toxicity 

Loo No. 2080 

Page 6 of l2 

Abstract 

The bacterial toxicity of the substance tested~~ ~vas tested by means of a respiration 

inhibition test on non-adapted activated sludge . 

The test was conducted on 06r'36/1991 accordin~~ to OECD guideline 209. 

7 concentrations were tested in a concentration range of 33-1 .000 [m`=/1] with a geometric factor of 1 .8 . 

The respiration rates of the control. reference and test preparations were determined after a 3-hour contact 

period and the inhibition effect of the test and reference concentrations were determined as a function of 

control respiration rates . 

The ratio of concentration to efficacy is graphically represented on a probability grid . 

To verifj~ the inoculurn and the test conditions ; a reference substance test was conducted with copper (ll) 

sulfate pentahydrate . 

The EC values were determined to be 320 [mg/1] by means of the graph of the ratio of concentration to 

efficacy . 

Up to 320 [mg/1] there is a linear, significant and normally-distributed increase of inhibition . Starting at 580 

[mQll], a decrease of urhibiting power was observed . This decrease can be attributed (for example) to 

maskinQ effects (stimulation, etc .) . 

Substance tested 

C C values 95% confidence interval 

EC2c, . 57 [mg/11 

98 [mg/1] 41 .3 < EC50 < 232.3 ECso 

EC$ : 160 [mg/1] 
p. 63 

DR U. NOACK LABORATORY FOR APPLIED BIOLOGY 

Report : Activated Sludge - 

Respiration Inhibition Test according, lo i Log No. 2080 

OECD Guideline 209 

I Test of Bacterial Toxicitr 

Substance tested 

r 

Method 

CAS No. : unknown 

Code none 

Batch no. 24726 

Consistencv : paste (according to data sheet) 

Solubility in water soluble, sliahtly cloudy (according to data 

Stabilit~~ : > 2 vears (arrnrriinR to data sheet) ~ 

Client : 

l 

sheet) 

l . Test guidelines : OECD guideline 209 and GLP yruidelines 

(BOB] of 03!22/1990, No. 13, addendum 1 . page 539 et seq .) 

2. Test facility : DR. U. NOACK LABORATORY 

FOR APPLIED BIOLOGY 

Richthofenstr. 29. 3200 Hildesheim 

2.1 Study Director : M. Noack (Biol .) 

2.2 Administrator : I. Rautenberg 

3 . Test system : Activated sludge 

3 .1 Source : Hildesheim municipal wastewater treatment plant 

3 .2 Pre-treatment : After sedimentation, washed twice with dechlorinated tap water 

(autoclaved) 

3.3 Culture medium : Synthetic wastewater according to OECD 209 

3 .4 Dry weight of activated sludge : 2.92 [g/1] 

4. Test receptacle : Narrow-necked Erlenmeyer flask; 

500 ml rated volume . DIN 12380 

4.1 Volume of the test medium : 500 ml 

4 .2 Water : All preparations [made] with dechlorinated tap water (treated by 

autoclave) 

5. Aeration : Constant aeration with compressed air 

Page 7 of 12 

6. Solubilizer : Solubilizer was not used 
DR U NOACK LABORATORY FOR AP PLIED BIOLOGY 

Report : Activated SludgeRespiration Inhibition Test according to 

OECD Guidelinc 209 

Test of Bacterial ToaicitY 

: Copper (11) sulfate-pentahydrate, p.a . (Merck) 

: 58, 100, 180 [mg/I] . factor 1 .8 

: 58. 100, 180 [m2/11 

: Individual preparations 

3 hours 

: 6126/1991 

Method 

7 . Reference substance 

7 .1 Concentration ranLe 

7 .'_' Test concentrations 

7 .3 Stock solution 

7 .4 Test duration 

7 .5 Test date 

8. Substance tested 

Code 

8.1 Preliminarv test on 

Concentrations 

Main test 

8? Concentration range 

Test concentrations 

Control preparation 

Stock solution 

Solubilit,~ 

Dispersing procedure 

Test date 

Test duration 

Test temperature 

9 . Measurement of parameters 

10 . Measurement procedures 

11 . Measurement equipment 

: unknown 

: 6!5/1991 

: 10 ., 100 [m~/1] 

Lou , No. 2080 

Page 8 of 12 

: 32-1000 [mg/1] . factor 1 .8 

: 32, 58 . 100. 180. 320. 580. 1000 [mg/11 

: Culture medium with inoculum 

: Individual preparations of the test concentrations 

: Up to l [Q/1] dissolved in cloudy solution 

: none 

: 6L)6!] 991 

: 3 hours 

: 18-2?°C 

: Temperature of the test preparations before and during the 

main test 

: Continuous oxygen analysis by means of oxygen electrodes 

(polarographic) and voltage recorder 

: Oxygen : Oxi 530 Oximeter, (WTW) with 

temperature correction 

pH value . Corning pH-meter, pH 240 

Temperature : Oxi 530 Oximeter, (VVTW) with 

temperature correction 

12 . Evaluation 

- Calculation of respiration rates of the test and control preparations and deviation from the control 

preparation (for calculations, see page 10 . in Results) 

- Graph of the ratio of concentration to efficacy on a probability 0-1-id 

- Statistical methods according to Ausgewdhlte Metlaoden der N'asseruntersuchu»g [Selected Methods 

of Water Analysis]. volume 11 (1982), 379-382, VEB Fischer Verlag, publishers 
p. 65 

DR U NOACK LABORATORY FOR APPLIED BIOLOGY 

Report: Activated SludgeRespiration lnhibition Test accordinc to Log No. 2080 

OECD Guideline 209 

Test of' Bacterial Toxicitv 

Page 9 of' 12 

Results 

1 . Toxicity of the reference substance : Copper (if) sulfate pentahydrate, p .a . (Merck) 

Inhibition at concentration 100 (ing/1] : 46 [°~o] 

Standard of proof: Inhibition between 40 and 60°rb 

Toxicity of the substance tested 

EC values 95'I, confidence interval 

ECZo : 57 [m,-/I] 

98 [MR/1] 41 .3 < ECso < 233 .3 EC, g, 

ECSO : 160 [m~ /I] 

3 . Quality criteria 

The following test guideline quality criteria were fulfilled : 

- Deviation of control respiration rates < 1 5 [°/o] 

- Inhibition effect of reference concentration 100 [mg/1] : 40-60 [°~o] 
p. 66 

DR U NOACK LABORATORY FOR APPLIED BI OLOGY 

Report: Activated SludgeRespiration Inhibition Test according to 

OECD Guideline 209 

Test of Bacterial Toxicity 

Log No . 2(t8t1 

Page 10 of 12 

Results 

Table 1 : Calculation of respiration rates and inhibition effect of the 

su s 

Concentration Respiration rate Inhibition 

[mz/1] [inc 0,/1 " h] [°~o] 

Substance tested 1000 2 

580 17 .6 47 

320 5 .? 84 

180 9.2 72 

100 10.8 68 

58 24.0 28 

32 31 .6 5 

Reference substance 180 8 .4 75 

100 18 .0 46 

58 29.2 13 

Control K 1 -- I 34 .0 I -- I 

Control K 2 

I 

-- '2 .8 -- 

Deviation of control respiration rates (Kl/K2) : 4 [%] 

Test temperature: 22°C 

Calculation of respiration rates (R) and inhibition (H) : k = !_tng Oz / .i - s 1 

b tance tested and reference substance concentrations 

7 - 

~' R 1 

- ; 100 

RFQ = Ri:2 i . 

, 

R = respiration rate of the evaluated test concentrations 

RK, = respiration rate of control 1 

RK= = respiration rate of control 2 
DR. U NOACK LABORATORY FOR APPLIED BIOLOGY 

Report : Activated SludgeRespiration Inhibition Test according to Log No. 2080 

OECD Guideline 209 

Test of Bacterial Toxicity ~ f'~~ be ~ 1 of' 12 

Results 

4 . Remarks 

4 .1 In a departure from OECD guideline 209 . 3,5-dichlorophenol was not used as a reference substance : 

instead, copper (11) sulfate pentahydrate was used . The concentration used produced a 50°rb inhibition 

of respiration. 

4.? Note: the substance tested foamed in all test preparations . They were prepared in concentrations of 

58-1000 [mu/I] . including those from SF-320 [mg/l] together with activated sludge, in concentrations 

580-1000 [mg/1] virtually only the test substance . 

4 .3 Graph on a probability --rid of the ratio of concentration to efficacy : see page 12 

4 .4 Test plan : see appendix 

4 .5 The test was conducted in compliance with OECD 209 and the GLP auidelines (with the exception 

noted in point 4 .1). 
DR U . NOACY. LABORATORY FOR APPLIED BIOLOGY 

Report: Activated Sludge -

Respiration Inhibition Test 

according to OECD Guideline 209 

Test of Bacterial Toxicity 

Log No . 2080 

Page 12 of 12 

Wahrscheinlichkeitsnetz = probability grid 

Haufigkeitssummen in °/~ der Gesamtzahl = Cumulative fi-equency in °1b of total 

f illegible term in rig lit margin] 

DR.IANOACh:-LABORATORIUN FUR ANGEWANDTE BIOLOGIE 

8ericht : Belebtschiamm-Respirationshemmtest 

nach OECD - Guideline 209 

Prutung auf Bakterientoxizitat 

97 

W 

+s 

»rt 

Journal-Nr . 

ZOBO 

Seite tp von 12 

I I-FT77MI 

W-1 

m mp 

Mm 7 

- 

=EF 

Q 

-j10 

to too low 

100 1000 10000 

[mg/1] 
DR U NOACY LABORATORY FOR APP LIED BIOLOGY 

[stamps] 

Copy matches original 

8/23/91 [initials] 

Appendix No. 1 

Page 1 of 3 

initials 

Activated SludgeRespiration Inhibition Test according to 

OECD Guideliue 209 

Test of Bacterial Toxicitly 

1 . Substance tested 

1 .1 Batch 

1 .2 Code : 

1 .3 Solubility 

1 .4 Volatility 

1 .5 Stability 

~-24726 

Log No. 2080 

Page l of 3 

Testing Plan 

unknown 

soluble, slightly cloud), (according to data sheet) 

unknown (accordin~= to data sheet) 

> 2 years (according to data sheet) 

unknown 1 .6 Manufacturer 

2 . Client 

2.1 Address 

3 . Test facility : DR. U. NOACh LABORATORY FOR APPLIED BIOLOGY 

RichthofenstT. 29 . D-3300 Hildesheim 

3 .1 Studv director : M. Noack 

3.? Administrator : I. Rautenberg 

4 . Test guidelines : OECD guideline 209 and GLP guidelines 

4 .1 Test system and source : Aerobic, aqueous medium with inoculum: 

mixed bacterial population (activated sludge) 

from the Hildesheim municipal wastewater treatment plant 

4.2 Test vessels : 500 ml narrow-necked Erlemneyer flasks 

4.3 Test volume : 500 ml 

4 .4 Number : One test preparation per concentration 

4 .5 Culture medium : Synthetic wastewater as under OECD 209 

4 .6 Temperature : Range of 18-22 degrees Celsius 

4 .7 Aeration : Permanent aeration by compressed air 

4 .8 Water : All preparations with dechlorinated tap water (autoclaved) 

p. 69 

5 . Type of measurements : Continuous measurement of oxygen content, by polarography 
DR U NOACK LABORATORY FOR APPLIED BIOLOGY 

[stamp] 

Appendix No . 1 

Page 2 of 3 

initials 

Activated SludgeRespiration Inhibition Test according to 

OECD Guideline 209 

Loo No . 2080 

Test of Bacterial Toxicity 

1'age 2 of 3 

Testing plan 

6. Preliminary test on : 6'5/1991 

6.1 Concentrations : 100, 10 [may/1] 

7 Main test 

7 .1 Test date : Calendar weeks 26-30 . 1991 

7 .2 Reference substance : Copper (II) sulfate pentahydrate p.a ., manufactured by Merck. 

Test concentrations : 58. 100 . 180 [mg/1] 

Stock solution : Individual preparations 

7 .3 Control test At beQinnina and end of trials 

7 .4 Substance tested 

Concentration range [ma/11 : 32-1000: factor 1 .8 

Test concentrations [mo/1] : 3'_' . 58, 100. 180. 3?0. 580 . 1000 [mgll] 

Stock solution [mg/1) : Individual preparations of test concentrations 

7 .5 Procedures according to standard operating procedure SOP OECD 209 

8 . Filing : Solubilitl~ trials, weighing records. unedited minutes (prelimmary 

and main test), recorder data . test report, test report diskette, 

microfiches 

9 . The Testing Plan was submitted to the Study Director on 6,'21/1991 by the Administrator. 

Hildesheim . 06i21/91 [signature] 

(Date) Stud), Director 

Hildesheim, 6/21/91 [signature] 

(Date) Director of Testing Facility 
DR U . NOACK LABORATORY FOR APPLIED BIOLOGY 

Activated Sludge - 

Respiration Inbibition Test according to 

OECD Guideline 209 

Test of Bacterial Toxicity 

Log No . 2080 

Page 3 of 3 

Certificate 

p. 71 

[stamp] 

Appendix No. 1 

Page 3 of 3 

initials 

We. the undersigned, hereby declare that this test was conducted in compliance with the principles of Good 

Laboraton~ Practice (GLP) . ~ 

All information, including that supplied b~~ ,-~~ill be treated as confidential . 

This includes all data that were recorded in the course of cond.uetinR thie qtnrivn 

All reports and results related to this study are the property 

The LABORATORY OF DR. NOACK may not disclose sai data to third parties without express 

aareement in writing. 

Hildesheim, 06,'21/91 [signature] 

(Date) Study Director 

[stamp] DR . U . NOACK LABORATORY 

FOR APPLIED BIOLOGY 

Richthofenstraf3e 29 

D-3200 Hildesheim 

Hildesheim . 6,'21/91 [signature] 

(Date) Director of Testing Facility 

FrankfurJ am Main -80 6,C 7/91 [signature] Kahne 

Place Date Client 

[stamps] Kahne 

MTH/ATA-TH TVS 

C 655, Te. 5878 
[stamp] 

Copy matches original 

8/23/91 [initials] 

Appendix No . 2 

Page 1 of 1 

[initials] 

To : 

Dr. U Noack Laboraton~ 

for Applied Biology 

Richthofenstrasse ?9 

3200 Hildesheirn 

--------------------- 

Dear Sirs : 

r 

We offer the enclosed sample for determination of its bacterial toxicity either (depending on its solubility) 

by: 

-- Respiration inhibition test as under OECD test guideline 209 or 

-- Brinkmann-Kuhn test (DFNI 38 412 part 8) 

in accordance with GLP principles . 

Trade name of the sample : I 

Composition: 

Batch identification : 247 ,26 

Physical data : 

Consistency: paste Density: 0.95 (50°C) 

Flash point: 180°C pH value (10 R/l) : 6-7 

Solubilit~~ in water: soluble (slightly cloudy) 

Solubilitv in organic solvents : ethanol, isopropanol 

Stability: >2 years (under normal conditions) 

Please send us the test report in triplicate along with your invoice. 

Sincerely, 

Frankfurt am Main. 5/2/91 by order [signature] 

(Place/Date) (Telephone) (Signature and Name stamp) i 
I)R t NCiACK LAHC)ItAI-OPI' 1=UR APPLII :I) F31 OLOGl' 

[stamp] 

Copy matches original 

8/23/91 [initials] 

Appendix No. 3 

Page 1 of 1 

[initials] 

Certificate 

-, 

iereby certifies that the 

[substances/mixtures specified in the attachment are to be tested for "bacteria] roxx-ik~ under GLP 

requirements according to OECD Guideline 209 or DIN Guideline 3f;41-1 part 8. however no concentration 

monitoring analysis is required . 

The client waives additional substance data in the form of an information sheet to the contractor and 

likewise waives the production of analysis certificates . 

The study should ascertain toxicity data for purposes of classification into its proper category as a hazard to 

water stocks . 

Frankfurt and Hildesheim, 

(Date) (Date) 

6/24/91 6,119,11991 

[[signature] [signature] 

SiQnature Signature 

(Client/stamp) (Director of the Testing Facilit),) 

Enclosures : List dated 5/8/1991 

List dated of 5/17/1991