Printing Date and Time:

 9/2/2011; 8:40 AM

k110728a.xls PFOA Page 1 of 2

Sample Name Sample ID Sample Comment Sample Type

Analyte 

Peak 

Name

Analyte 

Peak Area 

(counts)

Analyte 

Retention 

Time (min)

Analyte 

Conc. 

(ng/mL)

IS Peak 

Name

IS Peak 

Area 

(counts)

IS 

Retention 

Time (min)

IS Conc 

(ng/mL)

Use 

Record

Calculated 

Conc. 

(ng/mL)

Accuracy 

(%)

Record 

Modified

Area 

Ratio

k110728a001 11 005-59 Method Blank Unknown PFOA 45310 15.7 N/A 13C8PFOA-IS 1699356 15.7 1 0.00919 N/A 1 0.0267

k110728a002 11 005-59 Method Blank Unknown PFOA 18464 15.8 N/A 13C8PFOA-IS 1699010 15.8 1 < 0 N/A 1 0.0109

k110728a003 11 003-25-8 1.0 ng/mL FC std in Synth. Water Unknown PFOA 1637376 15.7 N/A 13C8PFOA-IS 1474998 15.7 1 1 N/A 0 1.11

k110728a004 11 003-25-8 1.0 ng/mL FC std in Synth. Water Unknown PFOA 1626052 15.8 N/A 13C8PFOA-IS 1455281 15.7 1 1.01 N/A 1 1.12

k110728a005 11 003-25-8 1.0 ng/mL FC std in Synth. Water Unknown PFOA 1620133 15.7 N/A 13C8PFOA-IS 1475256 15.8 1 0.993 N/A 1 1.1

k110728a006 11 003-25-8 1.0 ng/mL FC std in Synth. Water Unknown PFOA 1638391 15.7 N/A 13C8PFOA-IS 1464774 15.7 1 1.01 N/A 0 1.12

k110728a007 11 005-59 Method Blank Unknown PFOA 31366 15.8 N/A 13C8PFOA-IS 1771057 15.7 1 0.000989 N/A 1 0.0177

k110728a008 11 005-59 Method Blank Unknown PFOA 25497 15.8 N/A 13C8PFOA-IS 1786156 15.8 1 < 0 N/A 0 0.0143

k110728a009 11 003-25-1 0.025 ng/mL FC std in Synth. Water Standard PFOA 74908 15.8 0.025 13C8PFOA-IS 1639150 15.8 1 1 0.0266 106 0 0.0457

k110728a010 11 003-25-2 0.03 ng/mL FC std in Synth. Water Standard PFOA 80824 15.7 0.03 13C8PFOA-IS 1619851 15.8 1 1 0.0305 102 1 0.0499

k110728a011 11 003-25-3 0.04 ng/mL FC std in Synth. Water Standard PFOA 88971 15.8 0.04 13C8PFOA-IS 1419396 15.8 1 1 0.0422 105 1 0.0627

k110728a012 11 003-25-4 0.05 ng/mL FC std in Synth. Water Standard PFOA 112631 15.8 0.05 13C8PFOA-IS 1508101 15.8 1 1 0.0532 106 1 0.0747

k110728a013 11 003-25-5 0.1 ng/mL FC std in Synth. Water Standard PFOA 169690 15.8 0.1 13C8PFOA-IS 1389616 15.8 1 1 0.0966 96.6 1 0.122

k110728a014 11 003-25-6 0.25 ng/mL FC std in Synth. Water Standard PFOA 436010 15.8 0.25 13C8PFOA-IS 1498527 15.8 1 1 0.251 101 0 0.291

k110728a015 11 003-25-7 0.5 ng/mL FC std in Synth. Water Standard PFOA 737000 15.8 0.5 13C8PFOA-IS 1472276 15.8 1 1 0.444 88.7 1 0.501

k110728a016 11 003-25-8 1.0 ng/mL FC std in Synth. Water Standard PFOA 1661714 15.7 1 13C8PFOA-IS 1505817 15.7 1 1 0.998 99.8 1 1.1

k110728a017 11 003-25-9 2.5 ng/mL FC std in Synth. Water Standard PFOA 4341469 15.8 2.5 13C8PFOA-IS 1588870 15.8 1 1 2.5 100 0 2.73

k110728a018 11 003-25-10 5.0 ng/mL FC std in Synth. Water Standard PFOA 7643313 15.8 5 13C8PFOA-IS 1519360 15.7 1 1 4.65 93 1 5.03

k110728a019 11 003-25-11 10 ng/mL FC std in Synth. Water Standard PFOA 15458221 15.7 10 13C8PFOA-IS 1502764 15.7 1 1 9.66 96.6 1 10.3

k110728a020 11 003-25-12 15 ng/mL FC std in Synth. Water Standard PFOA 25929480 15.8 15 13C8PFOA-IS 1580609 15.8 1 1 15.7 105 1 16.4

k110728a021 11 003-25-13 25 ng/mL FC std in Synth. Water Standard PFOA 39149976 15.8 25 13C8PFOA-IS 1547418 15.8 1 1 24.9 99.6 0 25.3

k110728a022 11 003-25-14 50 ng/mL FC std in Synth. Water Standard PFOA 67094704 15.8 50 13C8PFOA-IS 1416930 15.7 1 1 50.5 101 1 47.4

k110728a023 11 003-25-15 75 ng/mL FC std in Synth. Water Standard PFOA 86780870 15.8 75 13C8PFOA-IS 1341564 15.8 1 1 75 100 1 64.7

k110728a024 11 003-25-16 100 ng/mL FC std in Synth. Water Standard PFOA 101094007 15.8 100 13C8PFOA-IS 1291097 15.7 1 1 99.5 99.5 1 78.3

k110728a025 11 005-59 Method Blank Unknown PFOA 31704 15.7 N/A 13C8PFOA-IS 1764847 15.7 1 0.00122 N/A 1 0.018

k110728a026 11 005-59 Method Blank Unknown PFOA 33646 15.8 N/A 13C8PFOA-IS 1791213 15.8 1 0.00197 N/A 1 0.0188

k110728a027 11 005-59 Method Blank Unknown PFOA 36789 15.8 N/A 13C8PFOA-IS 1789882 15.7 1 0.00359 N/A 1 0.0206

k110728a028 LCS-110726-1 0.2ppb LCS Quality Control PFOA 412430 15.8 0.199 13C8PFOA-IS 1666277 15.8 0.993 1 0.21 106 1 0.248

k110728a029 LCS-110726-2 0.2ppb LCS Quality Control PFOA 414795 15.8 0.199 13C8PFOA-IS 1671958 15.8 0.993 1 0.211 106 0 0.248

k110728a030 LCS-110726-3 0.2ppb LCS Quality Control PFOA 423595 15.8 0.199 13C8PFOA-IS 1634847 15.8 0.993 1 0.221 111 0 0.259

k110728a031 LCS-110726-4 2ppb LCS Quality Control PFOA 3727598 15.8 1.99 13C8PFOA-IS 1665098 15.8 0.993 1 2.03 102 0 2.24

k110728a032 LCS-110726-5 2ppb LCS Quality Control PFOA 3606350 15.8 1.99 13C8PFOA-IS 1664851 15.8 0.993 1 1.97 98.7 0 2.17

k110728a033 LCS-110726-6 2ppb LCS Quality Control PFOA 3675694 15.8 1.99 13C8PFOA-IS 1647352 15.7 0.993 1 2.02 102 1 2.23

k110728a034 LCS-110726-7 20ppb LCS Quality Control PFOA 31707235 15.8 19.9 13C8PFOA-IS 1593904 15.8 0.993 1 19.1 96 0 19.9

k110728a035 LCS-110726-8 20ppb LCS Quality Control PFOA 31945063 15.8 19.9 13C8PFOA-IS 1590940 15.7 0.993 1 19.3 97 1 20.1

k110728a036 LCS-110726-9 20ppb LCS Quality Control PFOA 31998046 15.7 19.9 13C8PFOA-IS 1595849 15.8 0.993 1 19.3 96.8 1 20.1

k110728a037 11 005-59 Method Blank Unknown PFOA 26889 15.7 N/A 13C8PFOA-IS 1820622 15.7 1 < 0 N/A 0 0.0148

k110728a038 11 005-59 Method Blank Unknown PFOA 25955 15.7 N/A 13C8PFOA-IS 1816554 15.7 1 < 0 N/A 1 0.0143

k110728a039 11 003-25-10 5.0 ng/mL FC std in Synth. Water Quality Control PFOA 7704481 15.7 5 13C8PFOA-IS 1573011 15.7 1 1 4.53 90.5 1 4.9

k110728a040 11 005-59 Method Blank Unknown PFOA 24852 15.8 N/A 13C8PFOA-IS 1825942 15.7 1 < 0 N/A 0 0.0136

k110728a041 LCS-110726-10 0.2ppb LCS ECF Quality Control PFOA 408715 15.8 0.199 13C8PFOA-IS 1679052 15.7 0.993 1 0.206 104 1 0.243

k110728a042 LCS-110726-11 0.2ppb LCS ECF Quality Control PFOA 408813 15.8 0.199 13C8PFOA-IS 1615078 15.8 0.993 1 0.215 108 1 0.253

k110728a043 LCS-110726-12 0.2ppb LCS ECF Quality Control PFOA 410789 15.8 0.199 13C8PFOA-IS 1611575 15.7 0.993 1 0.217 109 1 0.255

k110728a044 LCS-110726-13 2ppb LCS ECF Quality Control PFOA 3558803 15.7 1.99 13C8PFOA-IS 1627025 15.7 0.994 1 1.99 99.8 1 2.19

k110728a045 LCS-110726-14 2ppb LCS ECF Quality Control PFOA 3644463 15.7 1.99 13C8PFOA-IS 1645278 15.7 0.994 1 2.01 101 1 2.22

k110728a046 LCS-110726-15 2ppb LCS ECF Quality Control PFOA 3840625 15.7 1.99 13C8PFOA-IS 1634014 15.7 0.994 1 2.14 107 1 2.35

k110728a047 LCS-110726-16 20ppb LCS ECF Quality Control PFOA 33487411 15.8 19.9 13C8PFOA-IS 1597202 15.7 0.993 1 20.2 102 1 21

k110728a048 LCS-110726-17 20ppb LCS ECF Quality Control PFOA 35432370 15.7 19.9 13C8PFOA-IS 1578231 15.8 0.993 1 21.7 109 1 22.5

k110728a049 LCS-110726-18 20ppb LCS ECF Quality Control PFOA 36608567 15.7 19.9 13C8PFOA-IS 1568495 15.7 0.993 1 22.7 114 1 23.3

k110728a050 11 005-59 Method Blank Unknown PFOA 30567 15.8 N/A 13C8PFOA-IS 1845602 15.8 1 < 0 N/A 0 0.0166

k110728a051 11 005-59 Method Blank Unknown PFOA 38974 15.7 N/A 13C8PFOA-IS 1876933 15.8 1 0.00379 N/A 1 0.0208

k110728a052 11 003-25-9 2.5 ng/mL FC std in Synth. Water Quality Control PFOA 4349976 15.7 2.5 13C8PFOA-IS 1601502 15.7 1 1 2.49 99.6 1 2.72

k110728a053 11 005-59 Method Blank Unknown PFOA 27907 15.8 N/A 13C8PFOA-IS 1910739 15.8 1 < 0 N/A 1 0.0146

k110728a054 11 005-59 Method Blank Unknown PFOA 24607 15.8 N/A 13C8PFOA-IS 1937279 15.8 1 < 0 N/A 0 0.0127

k110728a055 GLP10-01-01-19-009 DAL GW BPMW121 RS 110713 Unknown PFOA 25220 15.8 N/A 13C8PFOA-IS 1662420 15.8 1 < 0 N/A 1 0.0152

k110728a056 GLP10-01-01-19-001 DAL GW TRIP1 0 110713 Unknown PFOA 25074 15.8 N/A 13C8PFOA-IS 1845379 15.8 1 < 0 N/A 1 0.0136

k110728a057 GLP10-01-01-19-002 DAL GW TRIP1 LS 110713 Unknown PFOA 528502 15.8 N/A 13C8PFOA-IS 1834147 15.8 1 0.249 N/A 0 0.288

k110728a058 GLP10-01-01-19-003 DAL GW TRIP1 MS 110713 Unknown PFOA 1936289 15.8 N/A 13C8PFOA-IS 1820247 15.7 1 0.961 N/A 0 1.06

k110728a059 GLP10-01-01-19-004 DAL GW TRIP1 HS 110713 Unknown PFOA 35362427 15.8 N/A 13C8PFOA-IS 1693055 15.8 1 20.3 N/A 1 20.9

k110728a060 11 005-59 Method Blank Unknown PFOA 31545 15.8 N/A 13C8PFOA-IS 1935284 15.8 1 < 0 N/A 1 0.0163

k110728a061 GLP10-01-01-19-005 DAL GW BPMW47 0 110713 Unknown PFOA 611160 15.8 N/A 13C8PFOA-IS 1683175 15.8 1 0.317 N/A 1 0.363

k110728a062 GLP10-01-01-19-006 DAL GW BPMW47 DB 110713 Unknown PFOA 626117 15.8 N/A 13C8PFOA-IS 1188796 15.8 1 0.468 N/A 1 0.527

k110728a063 GLP10-01-01-19-007 DAL GW BPMW47 LS 110713 Unknown PFOA 1099434 15.8 N/A 13C8PFOA-IS 1700703 15.8 1 0.578 N/A 1 0.646

k110728a064 GLP10-01-01-19-008 DAL GW BPMW47 HS 110713 Unknown PFOA 2440460 15.8 N/A 13C8PFOA-IS 1613828 15.8 1 1.37 N/A 1 1.51
Printing Date and Time:

 9/2/2011; 8:40 AM

k110728a.xls PFOA Page 2 of 2

Sample Name Sample ID Sample Comment Sample Type

Analyte 

Peak 

Name

Analyte 

Peak Area 

(counts)

Analyte 

Retention 

Time (min)

Analyte 

Conc. 

(ng/mL)

IS Peak 

Name

IS Peak 

Area 

(counts)

IS 

Retention 

Time (min)

IS Conc 

(ng/mL)

Use 

Record

Calculated 

Conc. 

(ng/mL)

Accuracy 

(%)

Record 

Modified

Area 

Ratio

k110728a065 11 005-59 Method Blank Unknown PFOA 26492 15.8 N/A 13C8PFOA-IS 1949430 15.8 1 < 0 N/A 1 0.0136

k110728a066 11 005-59 Method Blank Unknown PFOA 27410 15.8 N/A 13C8PFOA-IS 2054986 15.8 1 < 0 N/A 1 0.0133

k110728a067 11 003-25-8 1.0 ng/mL FC std in Synth. Water Quality Control PFOA 1698452 15.8 1 13C8PFOA-IS 1520767 15.8 1 1 1.01 101 1 1.12

k110728a068 11 005-59 Method Blank Unknown PFOA 25386 15.8 N/A 13C8PFOA-IS 2015195 15.8 1 < 0 N/A 1 0.0126

k110728a069 GLP10-01-01-19-010 DAL GW BPMW121 0 110713 Unknown PFOA 142264 15.8 N/A 13C8PFOA-IS 1560845 15.8 1 0.0682 N/A 1 0.0911

k110728a070 GLP10-01-01-19-011 DAL GW BPMW121 DB 110713 Unknown PFOA 145498 15.8 N/A 13C8PFOA-IS 1739522 15.8 1 0.0614 N/A 1 0.0836

k110728a071 GLP10-01-01-19-012 DAL GW BPMW121 LS 110713 Unknown PFOA 3923022 15.8 N/A 13C8PFOA-IS 1711543 15.8 1 2.1 N/A 1 2.29

k110728a072 GLP10-01-01-19-013 DAL GW BPMW121 HS 110713 Unknown PFOA 30468366 15.7 N/A 13C8PFOA-IS 1433413 15.7 1 20.6 N/A 0 21.3

k110728a073 11 005-59 Method Blank Unknown PFOA 27032 15.8 N/A 13C8PFOA-IS 2349850 15.7 1 < 0 N/A 1 0.0115

k110728a074 11 005-59 Method Blank Unknown PFOA 29911 15.7 N/A 13C8PFOA-IS 2341856 15.7 1 < 0 N/A 1 0.0128

k110728a075 GLP10-01-01-18-069 DAL GW 135S 0 110628 Unknown PFOA 35968578 15.8 N/A 13C8PFOA-IS 1725937 15.8 1 20.2 N/A 1 20.8

k110728a076 GLP10-01-01-18-070 DAL GW 135S DB 110628 Unknown PFOA 38551791 15.7 N/A 13C8PFOA-IS 1727576 15.7 1 21.7 N/A 1 22.3

k110728a077 GLP10-01-01-18-073 DAL GW 135S HS 110628 Unknown PFOA 71352722 15.8 N/A 13C8PFOA-IS 1685240 15.8 1 44.3 N/A 1 42.3

k110728a078 11 005-59 Method Blank Unknown PFOA 32435 15.8 N/A 13C8PFOA-IS 2384386 15.8 1 < 0 N/A 1 0.0136

k110728a079 11 005-59 Method Blank Unknown PFOA 33555 15.8 N/A 13C8PFOA-IS 2465577 15.8 1 < 0 N/A 0 0.0136

k110728a080 11 003-25-11 10 ng/mL FC std in Synth. Water Quality Control PFOA 16908635 15.8 10 13C8PFOA-IS 1594960 15.8 1 1 9.97 99.7 1 10.6

k110728a081 11 005-59 Method Blank Unknown PFOA 22321 15.8 N/A 13C8PFOA-IS 2360416 15.8 1 < 0 N/A 0 0.00946
Printing Date and Time:

 9/2/2011; 8:40 AM

k110728a.xls Regressions Page 1 of 1

Regressions

Peak Name: 13C8PFOA-IS

Use as Internal Standard

Q1/Q3 Masses: 421.00/376.00 Da

Peak Name: PFOA

Internal Standard: 13C8PFOA-IS

Q1/Q3 Masses: 413.00/369.00 413.00/219 413.00/169.00 Da

Fit Quadratic Weighting 1 / x Iterate No

a0 0.0166

a1 1.09

a2 -0.00307

Correlation coefficient 0.9998

Use Area
Printing Date and Time

9/2/2011; 1

k110728a.xls System Suitability Page 1 of 1

System Suitability 

Data File Sample ID Area Ratio Retention Time

k110728a003 1.0 ng/mL FC std in Synth. Water 1.11 15.7

k110728a004 1.0 ng/mL FC std in Synth. Water 1.12 15.8

k110728a005 1.0 ng/mL FC std in Synth. Water 1.10 15.7

k110728a006 1.0 ng/mL FC std in Synth. Water 1.12 15.7

Average 1.11 15.7

%RSD 0.86 0.32

Acceptance criteria: Area count RSD <5%, Retention Time RSD <2%.

PFOA
Printing Date and Time: 

9/2/2011; 8:40 AM

k110728a.xls] CCVs Page 1 of 1

CCV Recoveries

Sample Name Sample Comment Sample ID

Spiked 

Conc. 

(ng/mL)

Calc. Conc. 

(ng/mL) %Rec.

k110728a039 5.0 ng/mL FC std in Synth. Water 11 003-25-10 5.00 4.53 90.5

k110728a052 2.5 ng/mL FC std in Synth. Water 11 003-25-9 2.50 2.49 99.6

k110728a067 1.0 ng/mL FC std in Synth. Water 11 003-25-8 1.00 1.01 101

k110728a080 10 ng/mL FC std in Synth. Water 11 003-25-11 10.0 9.97 99.7

PFOA
Printing Date and Time: 

9/2/2011; 8:40 AM

k110728a.xls Method Blanks Page 1 of 1

Method Blanks

PFOA

Sample Name Sample Comment Sample ID

k110728a001 Method Blank 11 005-59 0.0267 Warm-up blanks - not used for LOQ determination

k110728a002 Method Blank 11 005-59 0.0109 Warm-up blanks - not used for LOQ determination

k110728a007 Method Blank 11 005-59 0.0177

k110728a008 Method Blank 11 005-59 0.0143

k110728a025 Method Blank 11 005-59 0.0180

k110728a026 Method Blank 11 005-59 0.0188

k110728a027 Method Blank 11 005-59 0.0206

k110728a037 Method Blank 11 005-59 0.0148

k110728a038 Method Blank 11 005-59 0.0143

k110728a040 Method Blank 11 005-59 0.0136

k110728a050 Method Blank 11 005-59 0.0166

k110728a051 Method Blank 11 005-59 0.0208

k110728a053 Method Blank 11 005-59 0.0146

k110728a054 Method Blank 11 005-59 0.0127

k110728a060 Method Blank 11 005-59 0.0163

k110728a065 Method Blank 11 005-59 0.0136

k110728a066 Method Blank 11 005-59 0.0133

k110728a068 Method Blank 11 005-59 0.0126

k110728a073 Method Blank 11 005-59 0.0115

k110728a074 Method Blank 11 005-59 0.0128

k110728a078 Method Blank 11 005-59 0.0136

k110728a079 Method Blank 11 005-59 0.0136

k110728a081 Method Blank 11 005-59 0.00946

Average of area ratio of method blanks 0.0149

RSD of area ratio 20

Average of method blanks *2 0.0299

LOQ Standard area ratio 0.0457

LOQ standard conc., ng/mL 0.0250
Printing Date and Time: 

9/2/2011; 8:40 AM

k110728a.xls Lab Control Spikes Page 1 of 1

LCS Samples

Acceptance Criteria: Average Recovery of each spike level within 100% ± 20 with a RSD <20%

Sample Name Sample Comment Sample ID

Spiked 

Conc. 

(ng/mL)

Calc. 

Conc. 

(ng/mL) %Rec.

k110728a028 0.2ppb LCS LCS-110726-1 0.199 0.210 106

k110728a029 0.2ppb LCS LCS-110726-2 0.199 0.211 106

k110728a030 0.2ppb LCS LCS-110726-3 0.199 0.221 111

Set RSD/Avg 2.7 108

k110728a031 2ppb LCS LCS-110726-4 1.99 2.03 102

k110728a032 2ppb LCS LCS-110726-5 1.99 1.97 98.7

k110728a033 2ppb LCS LCS-110726-6 1.99 2.02 102

Set RSD/Avg 1.9 101

k110728a034 20ppb LCS LCS-110726-7 19.9 19.1 96.0

k110728a035 20ppb LCS LCS-110726-8 19.9 19.3 97.0

k110728a036 20ppb LCS LCS-110726-9 19.9 19.3 96.8

Set RSD/Avg 0.55 96.6

Overall Average 102

%RSD 5.1

ECF LCS

Sample Name Sample Comment Sample ID

Spiked 

Conc. 

(ng/mL)

Calc. 

Conc. 

(ng/mL) %Rec.

k110728a041 0.2ppb LCS ECF LCS-110726-10 0.199 0.206 104

k110728a042 0.2ppb LCS ECF LCS-110726-11 0.199 0.215 108

k110728a043 0.2ppb LCS ECF LCS-110726-12 0.199 0.217 109

Set RSD/Avg 2.5 107

k110728a044 2ppb LCS ECF LCS-110726-13 1.99 1.99 99.8

k110728a045 2ppb LCS ECF LCS-110726-14 1.99 2.01 101

k110728a046 2ppb LCS ECF LCS-110726-15 1.99 2.14 107

Set RSD/Avg 3.8 103

k110728a047 20ppb LCS ECF LCS-110726-16 19.9 20.2 102

k110728a048 20ppb LCS ECF LCS-110726-17 19.9 21.7 109

k110728a049 20ppb LCS ECF LCS-110726-18 19.9 22.7 114

Set RSD/Avg 5.6 108

Overall Average 106

%RSD 4.4

PFOA

PFOA
Printing Date and Time

9/2/2011; 8:40 AM

k110728a.xls Sample Data wQC (19 data) Page 1 of 1

Sample results with QC - Internal Standard Quantitation

NC = Not Calculated; Endogenous sample is greater than 2x spiking level

MW47

Datafile 3M LIMS ID Sample Description

 Conc. 

(ng/mL)

FMS 

%Recovery

k110728a061 GLP10-01-01-19-005 DAL GW BPMW47 0 110713 0.317 NA

k110728a062 GLP10-01-01-19-006 DAL GW BPMW47 DB 110713 0.468 NA

k110728a063 GLP10-01-01-19-007 DAL GW BPMW47 LS 110713 0.578 74.2

k110728a064 GLP10-01-01-19-008 DAL GW BPMW47 HS 110713 1.37 97.8

Sample will be reported without internal standard.

Average 0.393

Low Field Matrix Spike 0.250

High Field Matrix Spike 1.00

%RPD 38

MW121

Datafile 3M LIMS ID Sample Description

 Conc. 

(ng/mL)

FMS 

%Recovery

k110728a069 GLP10-01-01-19-010 DAL GW BPMW121 0 110713 0.0682 NA

k110728a070 GLP10-01-01-19-011 DAL GW BPMW121 DB 110713 0.0614 NA

k110728a071 GLP10-01-01-19-012 DAL GW BPMW121 LS 110713 2.10 102

k110728a072 GLP10-01-01-19-013 DAL GW BPMW121 HS 110713 20.6 103

Average 0.0648

Low Field Matrix Spike 2.00

High Field Matrix Spike 20.0

%RPD 10

Trip and Equipment Blanks

Datafile 3M LIMS ID Sample Description

 Conc. 

(ng/mL)

FMS 

%Recovery

k110728a055 GLP10-01-01-19-009 DAL GW BPMW121 RS 110713 <0.0250 NA

k110728a056 GLP10-01-01-19-001 DAL GW TRIP1 0 110713 <0.0250 NA

k110728a057 GLP10-01-01-19-002 DAL GW TRIP1 LS 110713 0.249 99.6

k110728a058 GLP10-01-01-19-003 DAL GW TRIP1 MS 110713 0.961 96.1

k110728a059 GLP10-01-01-19-004 DAL GW TRIP1 HS 110713 20.3 102

Low Field Matrix Spike 0.250

Mid Field Matrix Spike 1.00

High Field Matrix Spike 20.0

PFOA

PFOA

PFOA
Printing Date and Time

9/2/2011; 8:40 AM

k110728a.xls Sample Data wQC (18 data) Page 1 of 1

Sample results with QC - Internal Standard Quantitation

NC = Not Calculated; Endogenous sample is greater than 2x spiking level

135S

Datafile 3M LIMS ID Sample Description

 Conc. 

(ng/mL)

FMS 

%Recovery

k110728a075 GLP10-01-01-18-069 DAL GW 135S 0 110628 20.2 NA

k110728a076 GLP10-01-01-18-070 DAL GW 135S DB 110628 21.7 NA

k110728a077 GLP10-01-01-18-073 DAL GW 135S HS 110628 44.3 NC

Average 21.0

Low Field Matrix Spike 0.0985

Mid Field Matrix Spike 0.985

High Field Matrix Spike 9.85

%RPD 7.2

PFOA
Printing Date and Time

9/2/2011; 8:40 AM

k110728a.xls FMS (19) Page 1 of 1

Field Matrix Spike True Values

Stock ID PFOA PFBS PFHS PFOS

10 006-147 0.100 0.100 0.100 0.100

10 006-146 1.00 1.00 1.00 1.00

10 006-145 10.0 10.0 10.0 10.0

10 006-130 100 - - -

10 006-131 - 100 100 100

Spike Volume Final Volume

PFOA PFBS PFHS PFOS Stock ID (uL) (mL)

MW-47 Low 0.250 0.250 0.250 0.250 10 006-146 50 200

High 1.00 1.00 1.00 1.00 10 006-146 200 200

MW-121 Low 2.00 2.00 2.00 2.00 10 006-146 400 200

High 20.0 20.0 20.0 20.0 10 006-145 400 200

Trip Blank Low 0.250 0.250 0.250 0.250 10 006-146 50 200

Mid 1.00 1.00 1.00 1.00 10 006-146 200 200

High 20.0 20.0 20.0 20.0 10 006-145 400 200

Final Spike Concentration (ng/mL) = Volume Spike (uL)*Spike Solution Concentration (ug/mL)/Final Volume (mL)

Concentration (ug/mL)
Printing Date and Time

9/2/2011; 8:40 AM

k110728a.xls FMS (18) Page 1 of 1

Field Matrix Spike True Values

Concentration (ug/mL)

Stock ID PFOA

10 006-141 1.97

10 006-140 98.7

10 006-139 489

Final Spike Concentration (ng/mL)

PFOA Stock ID 1

Spike Volume 

(uL)

Final Volume 

(mL)

Low FMS 0.0985 10 006-141 10 200

Mid FMS 0.985 10 006-141 100 200

High FMS 9.85 10 006-141 1000 200

Low FMS 0.985 10 006-141 100 200

Mid FMS 9.85 10 006-141 1000 200

High FMS 98.7 10 006-140 200 200

Low FMS 9.85 10 006-141 1000 200

Mid FMS 98.7 10 006-140 200 200

High FMS 494 10 006-140 1000 200

Low FMS 98.7 10 006-140 200 200

High FMS 1956 10 006-139 800 200

Trip Blanks Low FMS 0.985 10 006-141 100 200

Mid FMS 9.85 10 006-141 1000 200

Mid High FMS 98.7 10 006-140 200 200

High FMS 494 10 006-140 1000 200

Final Spike Concentration (ng/mL) = Volume Spike (uL)*Spike Solution Concentratin (ug/mL)/Final Volume (mL)

134S, 135S, 136R, 137R, 137S, 137L 

and 138L

130L, 131S, 131L, 133R, 135L, 136S, 

136L, 141R, 142R, 143R, 144R[144S]

130R, 130S, 133S, 133L, 134L, 138R, 

138S, CW26C, CW26L and 140R 

131R, 134R and 135R
Sample Name: "k110728a001" Sample ID: "11 005-59" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 1 Sample Type: Unknown Concentration: N/A Calculated Conc: 0.00919 ng/mL Acq. Date: 7/28/2011 Acq. Time: 10:00:05 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.7 minArea: 45310 countsHeight: 6.96e+003 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e4

Intensity, cps

16.14

14.50

18.12

18.79

19.47

17.48

11.60

1.31 14.04

1.77

Sample Name: "k110728a001" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 1 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 10:00:05 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1699356 countsHeight: 2.84e+005 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e5

Intensity, cps

Sample Name: "k110728a002" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 2 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/28/2011 Acq. Time: 10:21:10 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 18464 countsHeight: 3.36e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e4

Intensity, cps

14.56

16.19

19.45

18.81

18.05

16.31

11.57 14.24

Sample Name: "k110728a002" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 2 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 10:21:10 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1699010 countsHeight: 3.04e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 1 of 41Printing Time: 2:34:58 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a003" Sample ID: "11 003-25-8" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "1.0 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 3 Sample Type: Unknown Concentration: N/A Calculated Conc: 1.00 ng/mL Acq. Date: 7/28/2011 Acq. Time: 10:42:14 AM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1637376 countsHeight: 2.96e+005 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e5

Intensity, cps

15.75

14.57

Sample Name: "k110728a003" Sample ID: "11 003-25-8" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "1.0 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 3 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 10:42:14 AM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1474998 countsHeight: 2.67e+005 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e5

Intensity, cps

Sample Name: "k110728a004" Sample ID: "11 003-25-8" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "1.0 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 4 Sample Type: Unknown Concentration: N/A Calculated Conc: 1.01 ng/mL Acq. Date: 7/28/2011 Acq. Time: 11:03:24 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 1626052 countsHeight: 2.68e+005 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e5

Intensity, cps

15.75

14.55

Sample Name: "k110728a004" Sample ID: "11 003-25-8" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "1.0 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 4 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 11:03:24 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1455281 countsHeight: 2.45e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 2 of 41Printing Time: 2:34:58 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a005" Sample ID: "11 003-25-8" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "1.0 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 5 Sample Type: Unknown Concentration: N/A Calculated Conc: 0.993 ng/mL Acq. Date: 7/28/2011 Acq. Time: 11:24:29 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.7 minArea: 1620133 countsHeight: 2.73e+005 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e52.7e5

Intensity, cps

15.75

14.54

Sample Name: "k110728a005" Sample ID: "11 003-25-8" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "1.0 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 5 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 11:24:29 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.8 minArea: 1475256 countsHeight: 2.65e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e5

Intensity, cps

Sample Name: "k110728a006" Sample ID: "11 003-25-8" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "1.0 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 6 Sample Type: Unknown Concentration: N/A Calculated Conc: 1.01 ng/mL Acq. Date: 7/28/2011 Acq. Time: 11:45:32 AM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1638391 countsHeight: 2.71e+005 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e52.7e5

Intensity, cps

15.74

14.53

Sample Name: "k110728a006" Sample ID: "11 003-25-8" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "1.0 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 6 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 11:45:32 AM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1464774 countsHeight: 2.33e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 3 of 41Printing Time: 2:34:59 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a007" Sample ID: "11 005-59" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 7 Sample Type: Unknown Concentration: N/A Calculated Conc: 0.000989 ng/mL Acq. Date: 7/28/2011 Acq. Time: 12:06:36 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 31366 countsHeight: 4.33e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e4

Intensity, cps

14.54

18.71

16.14

16.23

11.64 14.46

Sample Name: "k110728a007" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 7 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 12:06:36 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.7 minArea: 1771057 countsHeight: 2.73e+005 cpsStart Time: 15.6 minEnd Time: 16.1 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e52.7e5

Intensity, cps

Sample Name: "k110728a008" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 8 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/28/2011 Acq. Time: 12:27:43 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 25497 countsHeight: 4.50e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e42.0e42.1e4

Intensity, cps

14.54

16.16

18.80

18.19

17.44

14.29

11.75

Sample Name: "k110728a008" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 8 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 12:27:43 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1786156 countsHeight: 2.84e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 4 of 41Printing Time: 2:34:59 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a009" Sample ID: "11 003-25-1" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "0.025 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 9 Sample Type: Standard Concentration: 0.0250 ng/mL Calculated Conc: 0.0266 ng/mL Acq. Date: 7/28/2011 Acq. Time: 12:48:48 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 74908 countsHeight: 1.22e+004 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e4

Intensity, cps

14.53

14.58

15.77 18.80

16.17

19.41

18.71

16.36

1.35 11.59 14.26

1.73

Sample Name: "k110728a009" Sample ID: "11 003-25-1" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "0.025 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 9 Sample Type: Standard Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 12:48:48 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1639150 countsHeight: 2.68e+005 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e5

Intensity, cps

Sample Name: "k110728a010" Sample ID: "11 003-25-2" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "0.03 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 10 Sample Type: Standard Concentration: 0.0300 ng/mL Calculated Conc: 0.0305 ng/mL Acq. Date: 7/28/2011 Acq. Time: 1:09:59 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 80824 countsHeight: 1.33e+004 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e4

Intensity, cps

14.52

15.75

16.15

18.70

18.19

16.31

1.35

14.24 10.68 11.61

Sample Name: "k110728a010" Sample ID: "11 003-25-2" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "0.03 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 10 Sample Type: Standard Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 1:09:59 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1619851 countsHeight: 2.56e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 5 of 41Printing Time: 2:34:59 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a011" Sample ID: "11 003-25-3" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "0.04 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 11 Sample Type: Standard Concentration: 0.0400 ng/mL Calculated Conc: 0.0422 ng/mL Acq. Date: 7/28/2011 Acq. Time: 1:31:06 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 88971 countsHeight: 1.56e+004 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e4

Intensity, cps

14.55

15.76

18.76

16.15

18.14

18.68

19.43

17.98

14.69

1.35

1.79 11.66 14.16

Sample Name: "k110728a011" Sample ID: "11 003-25-3" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "0.04 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 11 Sample Type: Standard Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 1:31:06 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1419396 countsHeight: 2.58e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e5

Intensity, cps

Sample Name: "k110728a012" Sample ID: "11 003-25-4" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "0.05 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 12 Sample Type: Standard Concentration: 0.0500 ng/mL Calculated Conc: 0.0532 ng/mL Acq. Date: 7/28/2011 Acq. Time: 1:52:11 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 112631 countsHeight: 1.74e+004 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e42.0e4

Intensity, cps

14.55

15.76

18.73

16.16

18.83

17.17

1.35

11.61 14.38

Sample Name: "k110728a012" Sample ID: "11 003-25-4" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "0.05 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 12 Sample Type: Standard Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 1:52:11 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1508101 countsHeight: 2.50e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 6 of 41Printing Time: 2:34:59 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a013" Sample ID: "11 003-25-5" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "0.1 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 13 Sample Type: Standard Concentration: 0.100 ng/mL Calculated Conc: 0.0966 ng/mL Acq. Date: 7/28/2011 Acq. Time: 2:13:15 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 169690 countsHeight: 3.06e+004 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2000.04000.06000.08000.0

1.0e41.2e41.4e41.6e41.8e42.0e42.2e42.4e42.6e42.8e43.0e4

Intensity, cps

15.76

14.55

18.79

16.18

19.45

18.35

17.23

1.35

Sample Name: "k110728a013" Sample ID: "11 003-25-5" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "0.1 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 13 Sample Type: Standard Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 2:13:15 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.8 minArea: 1389616 countsHeight: 2.32e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e5

Intensity, cps

Sample Name: "k110728a014" Sample ID: "11 003-25-6" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "0.25 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 14 Sample Type: Standard Concentration: 0.250 ng/mL Calculated Conc: 0.251 ng/mL Acq. Date: 7/28/2011 Acq. Time: 2:34:19 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 436010 countsHeight: 7.58e+004 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5000.0

1.0e41.5e42.0e42.5e43.0e43.5e44.0e44.5e45.0e45.5e46.0e46.5e47.0e47.5e4

Intensity, cps

15.76

14.56

19.45

16.17 18.75

17.37

Sample Name: "k110728a014" Sample ID: "11 003-25-6" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "0.25 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 14 Sample Type: Standard Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 2:34:19 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1498527 countsHeight: 2.57e+005 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 7 of 41Printing Time: 2:34:59 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a015" Sample ID: "11 003-25-7" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "0.5 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 15 Sample Type: Standard Concentration: 0.500 ng/mL Calculated Conc: 0.444 ng/mL Acq. Date: 7/28/2011 Acq. Time: 2:55:24 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 737000 countsHeight: 1.30e+005 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5000.0

1.0e41.5e42.0e42.5e43.0e43.5e44.0e44.5e45.0e45.5e46.0e46.5e47.0e47.5e48.0e48.5e49.0e49.5e41.0e51.1e51.1e51.2e51.2e51.3e51.3e5

Intensity, cps

15.76

14.54

18.74

16.13

Sample Name: "k110728a015" Sample ID: "11 003-25-7" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "0.5 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 15 Sample Type: Standard Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 2:55:24 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.8 minArea: 1472276 countsHeight: 2.41e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e5

Intensity, cps

Sample Name: "k110728a016" Sample ID: "11 003-25-8" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "1.0 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 16 Sample Type: Standard Concentration: 1.00 ng/mL Calculated Conc: 0.998 ng/mL Acq. Date: 7/28/2011 Acq. Time: 3:16:32 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.7 minArea: 1661714 countsHeight: 2.65e+005 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e5

Intensity, cps

15.75

14.55

Sample Name: "k110728a016" Sample ID: "11 003-25-8" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "1.0 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 16 Sample Type: Standard Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 3:16:32 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1505817 countsHeight: 2.52e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 8 of 41Printing Time: 2:34:59 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a017" Sample ID: "11 003-25-9" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "2.5 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 17 Sample Type: Standard Concentration: 2.50 ng/mL Calculated Conc: 2.50 ng/mL Acq. Date: 7/28/2011 Acq. Time: 3:37:37 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 4341469 countsHeight: 6.81e+005 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5.0e41.0e51.5e52.0e52.5e53.0e53.5e54.0e54.5e55.0e55.5e56.0e56.5e5

Intensity, cps

15.77

Sample Name: "k110728a017" Sample ID: "11 003-25-9" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "2.5 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 17 Sample Type: Standard Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 3:37:37 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.8 minArea: 1588870 countsHeight: 2.50e+005 cpsStart Time: 15.7 minEnd Time: 16.1 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e5

Intensity, cps

Sample Name: "k110728a018" Sample ID: "11 003-25-10" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "5.0 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 18 Sample Type: Standard Concentration: 5.00 ng/mL Calculated Conc: 4.65 ng/mL Acq. Date: 7/28/2011 Acq. Time: 3:58:42 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 7643313 countsHeight: 1.12e+006 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.00

5.00e41.00e51.50e52.00e52.50e53.00e53.50e54.00e54.50e55.00e55.50e56.00e56.50e57.00e57.50e58.00e58.50e59.00e59.50e51.00e61.05e61.10e6

Intensity, cps

15.76

Sample Name: "k110728a018" Sample ID: "11 003-25-10" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "5.0 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 18 Sample Type: Standard Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 3:58:42 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1519360 countsHeight: 2.25e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 9 of 41Printing Time: 2:34:59 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a019" Sample ID: "11 003-25-11" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "10 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 19 Sample Type: Standard Concentration: 10.0 ng/mL Calculated Conc: 9.66 ng/mL Acq. Date: 7/28/2011 Acq. Time: 4:19:48 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.7 minArea: 15458221 countsHeight: 2.50e+006 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1.0e52.0e53.0e54.0e55.0e56.0e57.0e58.0e59.0e51.0e61.1e61.2e61.3e61.4e61.5e61.6e61.7e61.8e61.9e62.0e62.1e62.2e62.3e62.4e6

Intensity, cps

15.75

Sample Name: "k110728a019" Sample ID: "11 003-25-11" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "10 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 19 Sample Type: Standard Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 4:19:48 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.7 minArea: 1502764 countsHeight: 2.51e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e5

Intensity, cps

Sample Name: "k110728a020" Sample ID: "11 003-25-12" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "15 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 20 Sample Type: Standard Concentration: 15.0 ng/mL Calculated Conc: 15.7 ng/mL Acq. Date: 7/28/2011 Acq. Time: 4:40:55 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 25929480 countsHeight: 3.95e+006 cpsStart Time: 15.6 minEnd Time: 16.1 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e54.0e56.0e58.0e51.0e61.2e61.4e61.6e61.8e62.0e62.2e62.4e62.6e62.8e63.0e63.2e63.4e63.6e63.8e6

Intensity, cps

15.76

Sample Name: "k110728a020" Sample ID: "11 003-25-12" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "15 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 20 Sample Type: Standard Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 4:40:55 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1580609 countsHeight: 2.43e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 10 of 41Printing Time: 2:34:59 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a021" Sample ID: "11 003-25-13" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "25 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 21 Sample Type: Standard Concentration: 25.0 ng/mL Calculated Conc: 24.9 ng/mL Acq. Date: 7/28/2011 Acq. Time: 5:01:59 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 39149976 countsHeight: 5.91e+006 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5.0e51.0e61.5e62.0e62.5e63.0e63.5e64.0e64.5e65.0e65.5e6

Intensity, cps

15.76

Sample Name: "k110728a021" Sample ID: "11 003-25-13" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "25 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 21 Sample Type: Standard Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 5:01:59 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1547418 countsHeight: 2.55e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e5

Intensity, cps

Sample Name: "k110728a022" Sample ID: "11 003-25-14" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "50 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 22 Sample Type: Standard Concentration: 50.0 ng/mL Calculated Conc: 50.5 ng/mL Acq. Date: 7/28/2011 Acq. Time: 5:23:03 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 1 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 67094704 countsHeight: 9.14e+006 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5.0e51.0e61.5e62.0e62.5e63.0e63.5e64.0e64.5e65.0e65.5e66.0e66.5e67.0e67.5e68.0e68.5e69.0e6

Intensity, cps

15.75

Sample Name: "k110728a022" Sample ID: "11 003-25-14" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "50 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 22 Sample Type: Standard Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 5:23:03 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1416930 countsHeight: 2.15e+005 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 11 of 41Printing Time: 2:34:59 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a023" Sample ID: "11 003-25-15" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "75 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 23 Sample Type: Standard Concentration: 75.0 ng/mL Calculated Conc: 75.0 ng/mL Acq. Date: 7/28/2011 Acq. Time: 5:44:10 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 86780870 countsHeight: 1.17e+007 cpsStart Time: 15.6 minEnd Time: 16.1 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.00

5.00e51.00e61.50e62.00e62.50e63.00e63.50e64.00e64.50e65.00e65.50e66.00e66.50e67.00e67.50e68.00e68.50e69.00e69.50e61.00e71.05e71.10e71.15e7

Intensity, cps

15.76

Sample Name: "k110728a023" Sample ID: "11 003-25-15" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "75 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 23 Sample Type: Standard Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 5:44:10 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1341564 countsHeight: 2.15e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e5

Intensity, cps

Sample Name: "k110728a024" Sample ID: "11 003-25-16" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "100 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 24 Sample Type: Standard Concentration: 100. ng/mL Calculated Conc: 99.5 ng/mL Acq. Date: 7/28/2011 Acq. Time: 6:05:15 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 101094007 countsHeight: 1.24e+007 cpsStart Time: 15.7 minEnd Time: 16.2 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.00

5.00e51.00e61.50e62.00e62.50e63.00e63.50e64.00e64.50e65.00e65.50e66.00e66.50e67.00e67.50e68.00e68.50e69.00e69.50e61.00e71.05e71.10e71.15e71.20e7

Intensity, cps

15.76

Sample Name: "k110728a024" Sample ID: "11 003-25-16" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "100 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 24 Sample Type: Standard Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 6:05:15 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1291097 countsHeight: 1.93e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 12 of 41Printing Time: 2:35:00 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a025" Sample ID: "11 005-59" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 25 Sample Type: Unknown Concentration: N/A Calculated Conc: 0.00122 ng/mL Acq. Date: 7/28/2011 Acq. Time: 6:26:19 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.7 minArea: 31704 countsHeight: 5.06e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e4

Intensity, cps

14.56

18.80

16.14

18.60

18.03

1.35

11.60 13.31 1.74

Sample Name: "k110728a025" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 25 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 6:26:19 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1764847 countsHeight: 2.81e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e5

Intensity, cps

Sample Name: "k110728a026" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 26 Sample Type: Unknown Concentration: N/A Calculated Conc: 0.00197 ng/mL Acq. Date: 7/28/2011 Acq. Time: 6:47:24 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 33646 countsHeight: 4.72e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e42.0e4

Intensity, cps

14.54

18.80

16.15

18.61

18.07 19.45

11.74 13.76

Sample Name: "k110728a026" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 26 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 6:47:24 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.8 minArea: 1791213 countsHeight: 3.14e+005 cpsStart Time: 15.6 minEnd Time: 16.1 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 13 of 41Printing Time: 2:35:00 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a027" Sample ID: "11 005-59" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 27 Sample Type: Unknown Concentration: N/A Calculated Conc: 0.00359 ng/mL Acq. Date: 7/28/2011 Acq. Time: 7:08:28 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 36789 countsHeight: 5.10e+003 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e4

Intensity, cps

14.54

18.72

16.16

18.12

17.97

1.35

11.69 14.32 1.69

Sample Name: "k110728a027" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 27 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 7:08:28 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1789882 countsHeight: 3.01e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e5

Intensity, cps

Sample Name: "k110728a028" Sample ID: "LCS-110726-1" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "0.2ppb LCS" Annotation: ""

Sample Index: 28 Sample Type: QC Concentration: 0.199 ng/mL Calculated Conc: 0.210 ng/mL Acq. Date: 7/28/2011 Acq. Time: 7:29:32 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 412430 countsHeight: 6.98e+004 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5000.0

1.0e41.5e42.0e42.5e43.0e43.5e44.0e44.5e45.0e45.5e46.0e46.5e47.0e4

Intensity, cps

15.76

14.54

16.12 18.76

16.46

Sample Name: "k110728a028" Sample ID: "LCS-110726-1" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "0.2ppb LCS" Annotation: ""

Sample Index: 28 Sample Type: QC Concentration: 0.993 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 7:29:32 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.8 minArea: 1666277 countsHeight: 2.93e+005 cpsStart Time: 15.6 minEnd Time: 16.1 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 14 of 41Printing Time: 2:35:00 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a029" Sample ID: "LCS-110726-2" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "0.2ppb LCS" Annotation: ""

Sample Index: 29 Sample Type: QC Concentration: 0.199 ng/mL Calculated Conc: 0.211 ng/mL Acq. Date: 7/28/2011 Acq. Time: 7:50:40 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 414795 countsHeight: 6.55e+004 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5000.0

1.0e41.5e42.0e42.5e43.0e43.5e44.0e44.5e45.0e45.5e46.0e46.5e4

Intensity, cps

15.75

14.53

19.45 16.15

18.73 18.13

Sample Name: "k110728a029" Sample ID: "LCS-110726-2" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "0.2ppb LCS" Annotation: ""

Sample Index: 29 Sample Type: QC Concentration: 0.993 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 7:50:40 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1671958 countsHeight: 2.66e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e5

Intensity, cps

Sample Name: "k110728a030" Sample ID: "LCS-110726-3" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "0.2ppb LCS" Annotation: ""

Sample Index: 30 Sample Type: QC Concentration: 0.199 ng/mL Calculated Conc: 0.221 ng/mL Acq. Date: 7/28/2011 Acq. Time: 8:11:47 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 423595 countsHeight: 7.52e+004 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5000.0

1.0e41.5e42.0e42.5e43.0e43.5e44.0e44.5e45.0e45.5e46.0e46.5e47.0e47.5e4

Intensity, cps

15.76

14.57

16.13

18.76

16.28

Sample Name: "k110728a030" Sample ID: "LCS-110726-3" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "0.2ppb LCS" Annotation: ""

Sample Index: 30 Sample Type: QC Concentration: 0.993 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 8:11:47 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.8 minArea: 1634847 countsHeight: 2.87e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 15 of 41Printing Time: 2:35:00 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a031" Sample ID: "LCS-110726-4" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "2ppb LCS" Annotation: ""

Sample Index: 31 Sample Type: QC Concentration: 1.99 ng/mL Calculated Conc: 2.03 ng/mL Acq. Date: 7/28/2011 Acq. Time: 8:32:54 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 3727598 countsHeight: 5.93e+005 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5.0e41.0e51.5e52.0e52.5e53.0e53.5e54.0e54.5e55.0e55.5e5

Intensity, cps

15.76

Sample Name: "k110728a031" Sample ID: "LCS-110726-4" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "2ppb LCS" Annotation: ""

Sample Index: 31 Sample Type: QC Concentration: 0.993 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 8:32:54 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1665098 countsHeight: 2.70e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e5

Intensity, cps

Sample Name: "k110728a032" Sample ID: "LCS-110726-5" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "2ppb LCS" Annotation: ""

Sample Index: 32 Sample Type: QC Concentration: 1.99 ng/mL Calculated Conc: 1.97 ng/mL Acq. Date: 7/28/2011 Acq. Time: 8:54:00 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 3606350 countsHeight: 5.61e+005 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e53.2e53.4e53.6e53.8e54.0e54.2e54.4e54.6e54.8e55.0e55.2e55.4e5

Intensity, cps

15.78

Sample Name: "k110728a032" Sample ID: "LCS-110726-5" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "2ppb LCS" Annotation: ""

Sample Index: 32 Sample Type: QC Concentration: 0.993 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 8:54:00 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1664851 countsHeight: 2.53e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 16 of 41Printing Time: 2:35:00 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a033" Sample ID: "LCS-110726-6" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "2ppb LCS" Annotation: ""

Sample Index: 33 Sample Type: QC Concentration: 1.99 ng/mL Calculated Conc: 2.02 ng/mL Acq. Date: 7/28/2011 Acq. Time: 9:15:07 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 3675694 countsHeight: 5.99e+005 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5.0e41.0e51.5e52.0e52.5e53.0e53.5e54.0e54.5e55.0e55.5e5

Intensity, cps

15.76

Sample Name: "k110728a033" Sample ID: "LCS-110726-6" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "2ppb LCS" Annotation: ""

Sample Index: 33 Sample Type: QC Concentration: 0.993 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 9:15:07 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1647352 countsHeight: 2.65e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e5

Intensity, cps

Sample Name: "k110728a034" Sample ID: "LCS-110726-7" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "20ppb LCS" Annotation: ""

Sample Index: 34 Sample Type: QC Concentration: 19.9 ng/mL Calculated Conc: 19.1 ng/mL Acq. Date: 7/28/2011 Acq. Time: 9:36:14 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 31707235 countsHeight: 4.92e+006 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e54.0e56.0e58.0e51.0e61.2e61.4e61.6e61.8e62.0e62.2e62.4e62.6e62.8e63.0e63.2e63.4e63.6e63.8e64.0e64.2e64.4e64.6e64.8e6

Intensity, cps

15.76

Sample Name: "k110728a034" Sample ID: "LCS-110726-7" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "20ppb LCS" Annotation: ""

Sample Index: 34 Sample Type: QC Concentration: 0.993 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 9:36:14 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.8 minArea: 1593904 countsHeight: 2.51e+005 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 17 of 41Printing Time: 2:35:00 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a035" Sample ID: "LCS-110726-8" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "20ppb LCS" Annotation: ""

Sample Index: 35 Sample Type: QC Concentration: 19.9 ng/mL Calculated Conc: 19.3 ng/mL Acq. Date: 7/28/2011 Acq. Time: 9:57:23 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 1 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 31945063 countsHeight: 5.16e+006 cpsStart Time: 15.7 minEnd Time: 16.1 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e54.0e56.0e58.0e51.0e61.2e61.4e61.6e61.8e62.0e62.2e62.4e62.6e62.8e63.0e63.2e63.4e63.6e63.8e64.0e64.2e64.4e64.6e64.8e65.0e6

Intensity, cps

15.75

Sample Name: "k110728a035" Sample ID: "LCS-110726-8" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "20ppb LCS" Annotation: ""

Sample Index: 35 Sample Type: QC Concentration: 0.993 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 9:57:23 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.7 minArea: 1590940 countsHeight: 2.78e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e52.7e5

Intensity, cps

Sample Name: "k110728a036" Sample ID: "LCS-110726-9" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "20ppb LCS" Annotation: ""

Sample Index: 36 Sample Type: QC Concentration: 19.9 ng/mL Calculated Conc: 19.3 ng/mL Acq. Date: 7/28/2011 Acq. Time: 10:18:28 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 31998046 countsHeight: 5.17e+006 cpsStart Time: 15.6 minEnd Time: 16.1 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e54.0e56.0e58.0e51.0e61.2e61.4e61.6e61.8e62.0e62.2e62.4e62.6e62.8e63.0e63.2e63.4e63.6e63.8e64.0e64.2e64.4e64.6e64.8e65.0e6

Intensity, cps

15.75

Sample Name: "k110728a036" Sample ID: "LCS-110726-9" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "20ppb LCS" Annotation: ""

Sample Index: 36 Sample Type: QC Concentration: 0.993 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 10:18:28 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.8 minArea: 1595849 countsHeight: 2.70e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 18 of 41Printing Time: 2:35:00 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a037" Sample ID: "11 005-59" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 37 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/28/2011 Acq. Time: 10:39:32 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 26889 countsHeight: 4.22e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e4

Intensity, cps

14.55

18.73 16.18

16.14

18.41

14.70

11.59

Sample Name: "k110728a037" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 37 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 10:39:32 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1820622 countsHeight: 2.98e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e5

Intensity, cps

Sample Name: "k110728a038" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 38 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/28/2011 Acq. Time: 11:00:40 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.7 minArea: 25955 countsHeight: 4.94e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e4

Intensity, cps

14.55

18.71

16.13

18.22

17.40

1.35 11.63 14.28

Sample Name: "k110728a038" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 38 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 11:00:40 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1816554 countsHeight: 2.97e+005 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 19 of 41Printing Time: 2:35:00 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a039" Sample ID: "11 003-25-10" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "5.0 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 39 Sample Type: QC Concentration: 5.00 ng/mL Calculated Conc: 4.53 ng/mL Acq. Date: 7/28/2011 Acq. Time: 11:21:47 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.7 minArea: 7704481 countsHeight: 1.27e+006 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.00

5.00e41.00e51.50e52.00e52.50e53.00e53.50e54.00e54.50e55.00e55.50e56.00e56.50e57.00e57.50e58.00e58.50e59.00e59.50e51.00e61.05e61.10e61.15e61.20e61.25e6

Intensity, cps

15.74

Sample Name: "k110728a039" Sample ID: "11 003-25-10" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "5.0 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 39 Sample Type: QC Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 11:21:47 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1573011 countsHeight: 2.61e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e5

Intensity, cps

Sample Name: "k110728a040" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 40 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/28/2011 Acq. Time: 11:42:56 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 24852 countsHeight: 4.04e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e4

Intensity, cps

14.53

16.17

18.80

18.17

17.40

1.35 11.64 14.29 4.89

Sample Name: "k110728a040" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 40 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/28/2011 Acq. Time: 11:42:56 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1825942 countsHeight: 3.01e+005 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 20 of 41Printing Time: 2:35:00 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a041" Sample ID: "LCS-110726-10" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "0.2ppb LCS ECF" Annotation: ""

Sample Index: 41 Sample Type: QC Concentration: 0.199 ng/mL Calculated Conc: 0.206 ng/mL Acq. Date: 7/29/2011 Acq. Time: 12:04:08 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 408715 countsHeight: 5.67e+004 cpsStart Time: 15.5 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5000.0

1.0e41.5e42.0e42.5e43.0e43.5e44.0e44.5e45.0e45.5e4

Intensity, cps

15.76

14.55

18.81 16.15

18.24

Sample Name: "k110728a041" Sample ID: "LCS-110726-10" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "0.2ppb LCS ECF" Annotation: ""

Sample Index: 41 Sample Type: QC Concentration: 0.993 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 12:04:08 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1679052 countsHeight: 2.62e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e5

Intensity, cps

Sample Name: "k110728a042" Sample ID: "LCS-110726-11" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "0.2ppb LCS ECF" Annotation: ""

Sample Index: 42 Sample Type: QC Concentration: 0.199 ng/mL Calculated Conc: 0.215 ng/mL Acq. Date: 7/29/2011 Acq. Time: 12:25:16 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 408813 countsHeight: 6.11e+004 cpsStart Time: 15.5 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5000.0

1.0e41.5e42.0e42.5e43.0e43.5e44.0e44.5e45.0e45.5e46.0e4

Intensity, cps

15.75

14.54

18.76 16.14

18.26 19.44

Sample Name: "k110728a042" Sample ID: "LCS-110726-11" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "0.2ppb LCS ECF" Annotation: ""

Sample Index: 42 Sample Type: QC Concentration: 0.993 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 12:25:16 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1615078 countsHeight: 3.00e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 21 of 41Printing Time: 2:35:01 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a043" Sample ID: "LCS-110726-12" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "0.2ppb LCS ECF" Annotation: ""

Sample Index: 43 Sample Type: QC Concentration: 0.199 ng/mL Calculated Conc: 0.217 ng/mL Acq. Date: 7/29/2011 Acq. Time: 12:46:26 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 410789 countsHeight: 5.86e+004 cpsStart Time: 15.5 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5000.0

1.0e41.5e42.0e42.5e43.0e43.5e44.0e44.5e45.0e45.5e4

Intensity, cps

15.75

14.54

18.69

16.13

17.33

Sample Name: "k110728a043" Sample ID: "LCS-110726-12" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "0.2ppb LCS ECF" Annotation: ""

Sample Index: 43 Sample Type: QC Concentration: 0.993 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 12:46:26 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.7 minArea: 1611575 countsHeight: 2.67e+005 cpsStart Time: 15.6 minEnd Time: 16.1 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e5

Intensity, cps

Sample Name: "k110728a044" Sample ID: "LCS-110726-13" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "2ppb LCS ECF" Annotation: ""

Sample Index: 44 Sample Type: QC Concentration: 1.99 ng/mL Calculated Conc: 1.99 ng/mL Acq. Date: 7/29/2011 Acq. Time: 1:07:32 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.7 minArea: 3558803 countsHeight: 5.01e+005 cpsStart Time: 15.4 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e53.2e53.4e53.6e53.8e54.0e54.2e54.4e54.6e54.8e55.0e5

Intensity, cps

15.75

Sample Name: "k110728a044" Sample ID: "LCS-110726-13" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "2ppb LCS ECF" Annotation: ""

Sample Index: 44 Sample Type: QC Concentration: 0.994 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 1:07:32 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1627025 countsHeight: 2.81e+005 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 22 of 41Printing Time: 2:35:01 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a045" Sample ID: "LCS-110726-14" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "2ppb LCS ECF" Annotation: ""

Sample Index: 45 Sample Type: QC Concentration: 1.99 ng/mL Calculated Conc: 2.01 ng/mL Acq. Date: 7/29/2011 Acq. Time: 1:28:37 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.7 minArea: 3644463 countsHeight: 4.92e+005 cpsStart Time: 15.4 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e53.2e53.4e53.6e53.8e54.0e54.2e54.4e54.6e54.8e5

Intensity, cps

15.74

Sample Name: "k110728a045" Sample ID: "LCS-110726-14" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "2ppb LCS ECF" Annotation: ""

Sample Index: 45 Sample Type: QC Concentration: 0.994 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 1:28:37 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1645278 countsHeight: 2.76e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e52.7e5

Intensity, cps

Sample Name: "k110728a046" Sample ID: "LCS-110726-15" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "2ppb LCS ECF" Annotation: ""

Sample Index: 46 Sample Type: QC Concentration: 1.99 ng/mL Calculated Conc: 2.14 ng/mL Acq. Date: 7/29/2011 Acq. Time: 1:49:43 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.7 minArea: 3840625 countsHeight: 4.94e+005 cpsStart Time: 15.4 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e53.2e53.4e53.6e53.8e54.0e54.2e54.4e54.6e54.8e5

Intensity, cps

15.74

Sample Name: "k110728a046" Sample ID: "LCS-110726-15" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "2ppb LCS ECF" Annotation: ""

Sample Index: 46 Sample Type: QC Concentration: 0.994 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 1:49:43 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1634014 countsHeight: 2.53e+005 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 23 of 41Printing Time: 2:35:01 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a047" Sample ID: "LCS-110726-16" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "20ppb LCS ECF" Annotation: ""

Sample Index: 47 Sample Type: QC Concentration: 19.9 ng/mL Calculated Conc: 20.2 ng/mL Acq. Date: 7/29/2011 Acq. Time: 2:10:50 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 33487411 countsHeight: 4.33e+006 cpsStart Time: 15.4 minEnd Time: 16.1 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e54.0e56.0e58.0e51.0e61.2e61.4e61.6e61.8e62.0e62.2e62.4e62.6e62.8e63.0e63.2e63.4e63.6e63.8e64.0e64.2e6

Intensity, cps

15.75

Sample Name: "k110728a047" Sample ID: "LCS-110726-16" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "20ppb LCS ECF" Annotation: ""

Sample Index: 47 Sample Type: QC Concentration: 0.993 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 2:10:50 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1597202 countsHeight: 2.53e+005 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e5

Intensity, cps

Sample Name: "k110728a048" Sample ID: "LCS-110726-17" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "20ppb LCS ECF" Annotation: ""

Sample Index: 48 Sample Type: QC Concentration: 19.9 ng/mL Calculated Conc: 21.7 ng/mL Acq. Date: 7/29/2011 Acq. Time: 2:31:55 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.7 minArea: 35432370 countsHeight: 4.30e+006 cpsStart Time: 15.4 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e54.0e56.0e58.0e51.0e61.2e61.4e61.6e61.8e62.0e62.2e62.4e62.6e62.8e63.0e63.2e63.4e63.6e63.8e64.0e64.2e6

Intensity, cps

15.75

Sample Name: "k110728a048" Sample ID: "LCS-110726-17" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "20ppb LCS ECF" Annotation: ""

Sample Index: 48 Sample Type: QC Concentration: 0.993 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 2:31:55 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1578231 countsHeight: 2.37e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 24 of 41Printing Time: 2:35:01 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a049" Sample ID: "LCS-110726-18" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "20ppb LCS ECF" Annotation: ""

Sample Index: 49 Sample Type: QC Concentration: 19.9 ng/mL Calculated Conc: 22.7 ng/mL Acq. Date: 7/29/2011 Acq. Time: 2:53:01 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.7 minArea: 36608567 countsHeight: 4.83e+006 cpsStart Time: 15.4 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e54.0e56.0e58.0e51.0e61.2e61.4e61.6e61.8e62.0e62.2e62.4e62.6e62.8e63.0e63.2e63.4e63.6e63.8e64.0e64.2e64.4e64.6e64.8e6

Intensity, cps

15.74

Sample Name: "k110728a049" Sample ID: "LCS-110726-18" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "20ppb LCS ECF" Annotation: ""

Sample Index: 49 Sample Type: QC Concentration: 0.993 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 2:53:01 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.7 minArea: 1568495 countsHeight: 2.58e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e5

Intensity, cps

Sample Name: "k110728a050" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 50 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/29/2011 Acq. Time: 3:14:10 AM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 30567 countsHeight: 5.99e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e4

Intensity, cps

14.53

18.82

16.15

16.20

18.17

17.41

5.01 8.77

11.61 14.37

Sample Name: "k110728a050" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 50 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 3:14:10 AM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1845602 countsHeight: 3.12e+005 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 25 of 41Printing Time: 2:35:01 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a051" Sample ID: "11 005-59" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 51 Sample Type: Unknown Concentration: N/A Calculated Conc: 0.00379 ng/mL Acq. Date: 7/29/2011 Acq. Time: 3:35:23 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 38974 countsHeight: 4.18e+003 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e42.0e4

Intensity, cps

14.53

16.14

18.79

19.46 18.15

16.33

17.46

11.75 14.35

Sample Name: "k110728a051" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 51 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 3:35:23 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.8 minArea: 1876933 countsHeight: 3.28e+005 cpsStart Time: 15.6 minEnd Time: 16.1 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e53.2e5

Intensity, cps

Sample Name: "k110728a052" Sample ID: "11 003-25-9" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "2.5 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 52 Sample Type: QC Concentration: 2.50 ng/mL Calculated Conc: 2.49 ng/mL Acq. Date: 7/29/2011 Acq. Time: 3:56:27 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.7 minArea: 4349976 countsHeight: 7.18e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5.0e41.0e51.5e52.0e52.5e53.0e53.5e54.0e54.5e55.0e55.5e56.0e56.5e57.0e5

Intensity, cps

15.75

Sample Name: "k110728a052" Sample ID: "11 003-25-9" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "2.5 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 52 Sample Type: QC Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 3:56:27 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1601502 countsHeight: 2.77e+005 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e52.7e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 26 of 41Printing Time: 2:35:01 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a053" Sample ID: "11 005-59" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 53 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/29/2011 Acq. Time: 4:17:36 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 27907 countsHeight: 4.09e+003 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e4

Intensity, cps

14.54

16.16

18.71

18.14

16.32

17.40

9.49

11.69 14.22

Sample Name: "k110728a053" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 53 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 4:17:36 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1910739 countsHeight: 3.13e+005 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e5

Intensity, cps

Sample Name: "k110728a054" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 54 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/29/2011 Acq. Time: 4:38:44 AM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 24607 countsHeight: 4.17e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e42.0e4

Intensity, cps

14.59

18.77

16.17

18.61

18.1717.41

11.66

Sample Name: "k110728a054" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 54 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 4:38:44 AM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1937279 countsHeight: 3.17e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 27 of 41Printing Time: 2:35:01 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a055" Sample ID: "GLP10-01-01-19-009" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "DAL GW BPMW121 RS 110713" Annotation: ""

Sample Index: 55 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/29/2011 Acq. Time: 4:59:49 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 25220 countsHeight: 4.01e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e42.0e4

Intensity, cps

14.56

18.76

16.16

19.43

18.50

18.13

11.59 14.35

Sample Name: "k110728a055" Sample ID: "GLP10-01-01-19-009" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "DAL GW BPMW121 RS 110713" Annotation: ""

Sample Index: 55 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 4:59:49 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1662420 countsHeight: 3.01e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e5

Intensity, cps

Sample Name: "k110728a056" Sample ID: "GLP10-01-01-19-001" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "DAL GW TRIP1 0 110713" Annotation: ""

Sample Index: 56 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/29/2011 Acq. Time: 5:20:58 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 25074 countsHeight: 4.51e+003 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e42.0e4

Intensity, cps

14.57

18.76

16.17

14.64 18.25

16.36

5.01 11.65 14.42

Sample Name: "k110728a056" Sample ID: "GLP10-01-01-19-001" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "DAL GW TRIP1 0 110713" Annotation: ""

Sample Index: 56 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 5:20:58 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1845379 countsHeight: 3.30e+005 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e53.2e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 28 of 41Printing Time: 2:35:01 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a057" Sample ID: "GLP10-01-01-19-002" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "DAL GW TRIP1 LS 110713" Annotation: ""

Sample Index: 57 Sample Type: Unknown Concentration: N/A Calculated Conc: 0.249 ng/mL Acq. Date: 7/29/2011 Acq. Time: 5:42:04 AM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 528502 countsHeight: 8.47e+004 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5000.0

1.0e41.5e42.0e42.5e43.0e43.5e44.0e44.5e45.0e45.5e46.0e46.5e47.0e47.5e48.0e48.5e4

Intensity, cps

15.77

14.59

16.15 18.84 19.46

Sample Name: "k110728a057" Sample ID: "GLP10-01-01-19-002" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "DAL GW TRIP1 LS 110713" Annotation: ""

Sample Index: 57 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 5:42:04 AM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1834147 countsHeight: 3.00e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e5

Intensity, cps

Sample Name: "k110728a058" Sample ID: "GLP10-01-01-19-003" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "DAL GW TRIP1 MS 110713" Annotation: ""

Sample Index: 58 Sample Type: Unknown Concentration: N/A Calculated Conc: 0.961 ng/mL Acq. Date: 7/29/2011 Acq. Time: 6:03:11 AM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1936289 countsHeight: 2.94e+005 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e5

Intensity, cps

15.77

14.57

Sample Name: "k110728a058" Sample ID: "GLP10-01-01-19-003" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "DAL GW TRIP1 MS 110713" Annotation: ""

Sample Index: 58 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 6:03:11 AM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1820247 countsHeight: 2.91e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 29 of 41Printing Time: 2:35:01 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a059" Sample ID: "GLP10-01-01-19-004" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "DAL GW TRIP1 HS 110713" Annotation: ""

Sample Index: 59 Sample Type: Unknown Concentration: N/A Calculated Conc: 20.3 ng/mL Acq. Date: 7/29/2011 Acq. Time: 6:24:21 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 35362427 countsHeight: 5.08e+006 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e54.0e56.0e58.0e51.0e61.2e61.4e61.6e61.8e62.0e62.2e62.4e62.6e62.8e63.0e63.2e63.4e63.6e63.8e64.0e64.2e64.4e64.6e64.8e65.0e6

Intensity, cps

15.78

Sample Name: "k110728a059" Sample ID: "GLP10-01-01-19-004" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "DAL GW TRIP1 HS 110713" Annotation: ""

Sample Index: 59 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 6:24:21 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.8 minArea: 1693055 countsHeight: 2.46e+005 cpsStart Time: 15.7 minEnd Time: 16.1 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e5

Intensity, cps

Sample Name: "k110728a060" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 60 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/29/2011 Acq. Time: 6:45:29 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 31545 countsHeight: 4.19e+003 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e4

Intensity, cps

14.59

16.17 19.44 18.69

18.60

16.58

1.37 11.70 14.08

Sample Name: "k110728a060" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 60 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 6:45:29 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1935284 countsHeight: 3.22e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e53.2e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 30 of 41Printing Time: 2:35:02 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a061" Sample ID: "GLP10-01-01-19-005" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "DAL GW BPMW47 0 110713" Annotation: ""

Sample Index: 61 Sample Type: Unknown Concentration: N/A Calculated Conc: 0.317 ng/mL Acq. Date: 7/29/2011 Acq. Time: 7:06:35 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 611160 countsHeight: 7.68e+004 cpsStart Time: 15.5 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5000.0

1.0e41.5e42.0e42.5e43.0e43.5e44.0e44.5e45.0e45.5e46.0e46.5e47.0e47.5e4

Intensity, cps

15.77

14.59

10.99 18.77 16.22

17.89

Sample Name: "k110728a061" Sample ID: "GLP10-01-01-19-005" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "DAL GW BPMW47 0 110713" Annotation: ""

Sample Index: 61 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 7:06:35 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1683175 countsHeight: 2.61e+005 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e5

Intensity, cps

Sample Name: "k110728a062" Sample ID: "GLP10-01-01-19-006" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "DAL GW BPMW47 DB 110713" Annotation: ""

Sample Index: 62 Sample Type: Unknown Concentration: N/A Calculated Conc: 0.468 ng/mL Acq. Date: 7/29/2011 Acq. Time: 7:27:43 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 626117 countsHeight: 7.99e+004 cpsStart Time: 15.5 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5000.0

1.0e41.5e42.0e42.5e43.0e43.5e44.0e44.5e45.0e45.5e46.0e46.5e47.0e47.5e48.0e4

Intensity, cps

15.76

14.59

18.75

16.16 19.44

17.45

Sample Name: "k110728a062" Sample ID: "GLP10-01-01-19-006" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "DAL GW BPMW47 DB 110713" Annotation: ""

Sample Index: 62 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 7:27:43 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1188796 countsHeight: 1.85e+005 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 31 of 41Printing Time: 2:35:02 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a063" Sample ID: "GLP10-01-01-19-007" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "DAL GW BPMW47 LS 110713" Annotation: ""

Sample Index: 63 Sample Type: Unknown Concentration: N/A Calculated Conc: 0.578 ng/mL Acq. Date: 7/29/2011 Acq. Time: 7:48:55 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 3 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 1099434 countsHeight: 1.59e+005 cpsStart Time: 15.5 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e5

Intensity, cps

15.76

14.57

18.83 16.17

Sample Name: "k110728a063" Sample ID: "GLP10-01-01-19-007" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "DAL GW BPMW47 LS 110713" Annotation: ""

Sample Index: 63 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 7:48:55 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.8 minArea: 1700703 countsHeight: 2.81e+005 cpsStart Time: 15.7 minEnd Time: 16.1 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e5

Intensity, cps

Sample Name: "k110728a064" Sample ID: "GLP10-01-01-19-008" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "DAL GW BPMW47 HS 110713" Annotation: ""

Sample Index: 64 Sample Type: Unknown Concentration: N/A Calculated Conc: 1.37 ng/mL Acq. Date: 7/29/2011 Acq. Time: 8:10:02 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 2440460 countsHeight: 3.99e+005 cpsStart Time: 15.5 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e53.2e53.4e53.6e53.8e5

Intensity, cps

15.76

Sample Name: "k110728a064" Sample ID: "GLP10-01-01-19-008" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "DAL GW BPMW47 HS 110713" Annotation: ""

Sample Index: 64 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 8:10:02 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.8 minArea: 1613828 countsHeight: 2.81e+005 cpsStart Time: 15.6 minEnd Time: 16.1 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 32 of 41Printing Time: 2:35:02 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a065" Sample ID: "11 005-59" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 65 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/29/2011 Acq. Time: 8:31:12 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 26492 countsHeight: 4.30e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e42.0e4

Intensity, cps

14.58

16.17 18.65

19.44

18.39

11.73 14.04

Sample Name: "k110728a065" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 65 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 8:31:12 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1949430 countsHeight: 3.60e+005 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e53.2e53.4e53.6e5

Intensity, cps

Sample Name: "k110728a066" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 66 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/29/2011 Acq. Time: 8:52:23 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 27410 countsHeight: 4.19e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e4

Intensity, cps

14.57

18.80

16.22

18.16 19.45

17.45

1.37

11.63 14.13

Sample Name: "k110728a066" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 66 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 8:52:23 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 2054986 countsHeight: 3.47e+005 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e53.2e53.4e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 33 of 41Printing Time: 2:35:02 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a067" Sample ID: "11 003-25-8" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "1.0 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 67 Sample Type: QC Concentration: 1.00 ng/mL Calculated Conc: 1.01 ng/mL Acq. Date: 7/29/2011 Acq. Time: 9:13:29 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 1698452 countsHeight: 3.11e+005 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e5

Intensity, cps

15.76

14.57

Sample Name: "k110728a067" Sample ID: "11 003-25-8" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "1.0 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 67 Sample Type: QC Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 9:13:29 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1520767 countsHeight: 2.67e+005 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e5

Intensity, cps

Sample Name: "k110728a068" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 68 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/29/2011 Acq. Time: 9:34:38 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 25386 countsHeight: 4.08e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e42.0e4

Intensity, cps

14.57

16.15

18.73

18.37

18.08

17.36

1.37

11.70 14.14

Sample Name: "k110728a068" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 68 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 9:34:38 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 2015195 countsHeight: 3.47e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e53.2e53.4e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 34 of 41Printing Time: 2:35:02 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a069" Sample ID: "GLP10-01-01-19-010" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "DAL GW BPMW121 0 110713" Annotation: ""

Sample Index: 69 Sample Type: Unknown Concentration: N/A Calculated Conc: 0.0682 ng/mL Acq. Date: 7/29/2011 Acq. Time: 9:55:45 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 142264 countsHeight: 2.01e+004 cpsStart Time: 15.5 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e42.0e42.1e4

Intensity, cps

15.78

14.56

16.21

18.79

18.34 19.45

17.47

1.38

10.99

0.46 1.55 11.64 14.03

Sample Name: "k110728a069" Sample ID: "GLP10-01-01-19-010" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "DAL GW BPMW121 0 110713" Annotation: ""

Sample Index: 69 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 9:55:45 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.8 minArea: 1560845 countsHeight: 2.65e+005 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e5

Intensity, cps

Sample Name: "k110728a070" Sample ID: "GLP10-01-01-19-011" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "DAL GW BPMW121 DB 110713" Annotation: ""

Sample Index: 70 Sample Type: Unknown Concentration: N/A Calculated Conc: 0.0614 ng/mL Acq. Date: 7/29/2011 Acq. Time: 10:16:55 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 145498 countsHeight: 1.96e+004 cpsStart Time: 15.5 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e42.0e4

Intensity, cps

15.76 14.55

16.12 18.73

18.58

18.25

18.11

5.01

11.64

14.25

Sample Name: "k110728a070" Sample ID: "GLP10-01-01-19-011" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "DAL GW BPMW121 DB 110713" Annotation: ""

Sample Index: 70 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 10:16:55 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1739522 countsHeight: 2.76e+005 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e52.7e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 35 of 41Printing Time: 2:35:02 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a071" Sample ID: "GLP10-01-01-19-012" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "DAL GW BPMW121 LS 110713" Annotation: ""

Sample Index: 71 Sample Type: Unknown Concentration: N/A Calculated Conc: 2.10 ng/mL Acq. Date: 7/29/2011 Acq. Time: 10:38:00 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 3923022 countsHeight: 7.20e+005 cpsStart Time: 15.5 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5.0e41.0e51.5e52.0e52.5e53.0e53.5e54.0e54.5e55.0e55.5e56.0e56.5e57.0e5

Intensity, cps

15.77

Sample Name: "k110728a071" Sample ID: "GLP10-01-01-19-012" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "DAL GW BPMW121 LS 110713" Annotation: ""

Sample Index: 71 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 10:38:00 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.8 minArea: 1711543 countsHeight: 3.25e+005 cpsStart Time: 15.7 minEnd Time: 16.1 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e53.2e5

Intensity, cps

Sample Name: "k110728a072" Sample ID: "GLP10-01-01-19-013" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "DAL GW BPMW121 HS 110713" Annotation: ""

Sample Index: 72 Sample Type: Unknown Concentration: N/A Calculated Conc: 20.6 ng/mL Acq. Date: 7/29/2011 Acq. Time: 10:59:06 AM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 30468366 countsHeight: 4.84e+006 cpsStart Time: 15.5 minEnd Time: 16.1 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e54.0e56.0e58.0e51.0e61.2e61.4e61.6e61.8e62.0e62.2e62.4e62.6e62.8e63.0e63.2e63.4e63.6e63.8e64.0e64.2e64.4e64.6e64.8e6

Intensity, cps

15.74

Sample Name: "k110728a072" Sample ID: "GLP10-01-01-19-013" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "DAL GW BPMW121 HS 110713" Annotation: ""

Sample Index: 72 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 10:59:06 AM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1433413 countsHeight: 2.38e+005 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 36 of 41Printing Time: 2:35:02 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a073" Sample ID: "11 005-59" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 73 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/29/2011 Acq. Time: 11:20:16 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 27032 countsHeight: 4.52e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e4

Intensity, cps

14.52

16.15

18.75

19.44

18.15

16.43

1.38

11.64 14.34

Sample Name: "k110728a073" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 73 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 11:20:16 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 2349850 countsHeight: 3.95e+005 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e53.2e53.4e53.6e53.8e5

Intensity, cps

Sample Name: "k110728a074" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 74 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/29/2011 Acq. Time: 11:41:27 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.7 minArea: 29911 countsHeight: 5.42e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e4

Intensity, cps

14.51

16.13

18.76

18.33 18.63

18.10

16.07

17.45

1.38 11.65 14.03

Sample Name: "k110728a074" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 74 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 11:41:27 AM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 2341856 countsHeight: 4.03e+005 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e53.2e53.4e53.6e53.8e54.0e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 37 of 41Printing Time: 2:35:02 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a075" Sample ID: "GLP10-01-01-18-069" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "DAL GW 135S 0 110628" Annotation: ""

Sample Index: 75 Sample Type: Unknown Concentration: N/A Calculated Conc: 20.2 ng/mL Acq. Date: 7/29/2011 Acq. Time: 12:02:36 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 35968578 countsHeight: 4.46e+006 cpsStart Time: 15.3 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e54.0e56.0e58.0e51.0e61.2e61.4e61.6e61.8e62.0e62.2e62.4e62.6e62.8e63.0e63.2e63.4e63.6e63.8e64.0e64.2e64.4e6

Intensity, cps

15.76

15.62

Sample Name: "k110728a075" Sample ID: "GLP10-01-01-18-069" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "DAL GW 135S 0 110628" Annotation: ""

Sample Index: 75 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 12:02:36 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Base To Base Retention Time: 15.8 minArea: 1725937 countsHeight: 2.90e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e5

Intensity, cps

Sample Name: "k110728a076" Sample ID: "GLP10-01-01-18-070" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "DAL GW 135S DB 110628" Annotation: ""

Sample Index: 76 Sample Type: Unknown Concentration: N/A Calculated Conc: 21.7 ng/mL Acq. Date: 7/29/2011 Acq. Time: 12:23:44 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.7 minArea: 38551791 countsHeight: 5.01e+006 cpsStart Time: 15.3 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

2.0e54.0e56.0e58.0e51.0e61.2e61.4e61.6e61.8e62.0e62.2e62.4e62.6e62.8e63.0e63.2e63.4e63.6e63.8e64.0e64.2e64.4e64.6e64.8e6

Intensity, cps

15.73

Sample Name: "k110728a076" Sample ID: "GLP10-01-01-18-070" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "DAL GW 135S DB 110628" Annotation: ""

Sample Index: 76 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 12:23:44 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.7 minArea: 1727576 countsHeight: 3.03e+005 cpsStart Time: 15.6 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 38 of 41Printing Time: 2:35:02 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a077" Sample ID: "GLP10-01-01-18-073" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "DAL GW 135S HS 110628" Annotation: ""

Sample Index: 77 Sample Type: Unknown Concentration: N/A Calculated Conc: 44.3 ng/mL Acq. Date: 7/29/2011 Acq. Time: 12:44:50 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 71352722 countsHeight: 8.74e+006 cpsStart Time: 15.4 minEnd Time: 16.1 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

5.0e51.0e61.5e62.0e62.5e63.0e63.5e64.0e64.5e65.0e65.5e66.0e66.5e67.0e67.5e68.0e68.5e6

Intensity, cps

15.76

Sample Name: "k110728a077" Sample ID: "GLP10-01-01-18-073" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "DAL GW 135S HS 110628" Annotation: ""

Sample Index: 77 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 12:44:50 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1685240 countsHeight: 2.80e+005 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e52.6e52.7e5

Intensity, cps

Sample Name: "k110728a078" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 78 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/29/2011 Acq. Time: 1:05:57 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 32435 countsHeight: 5.87e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e42.0e42.1e42.2e4

Intensity, cps

14.56

16.13

18.77 16.23

18.13

17.40

14.50

1.37 11.69 13.93

Sample Name: "k110728a078" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 78 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 1:05:57 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 2384386 countsHeight: 4.04e+005 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e53.2e53.4e53.6e53.8e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 39 of 41Printing Time: 2:35:03 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a079" Sample ID: "11 005-59" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 79 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/29/2011 Acq. Time: 1:27:08 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 33555 countsHeight: 5.55e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e42.0e42.1e42.2e42.3e4

Intensity, cps

14.56

16.16

19.46 18.76

18.89

18.60

17.41

11.63 14.10

Sample Name: "k110728a079" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 79 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 1:27:08 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 2465577 countsHeight: 4.43e+005 cpsStart Time: 15.6 minEnd Time: 15.9 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e53.2e53.4e53.6e53.8e54.0e54.2e54.4e5

Intensity, cps

Sample Name: "k110728a080" Sample ID: "11 003-25-11" File: "k110728a.wiff"Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "10 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 80 Sample Type: QC Concentration: 10.0 ng/mL Calculated Conc: 9.97 ng/mL Acq. Date: 7/29/2011 Acq. Time: 1:48:16 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Manual Retention Time: 15.8 minArea: 16908635 countsHeight: 2.73e+006 cpsStart Time: 15.7 minEnd Time: 16.0 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1.0e52.0e53.0e54.0e55.0e56.0e57.0e58.0e59.0e51.0e61.1e61.2e61.3e61.4e61.5e61.6e61.7e61.8e61.9e62.0e62.1e62.2e62.3e62.4e62.5e62.6e62.7e6

Intensity, cps

15.77

Sample Name: "k110728a080" Sample ID: "11 003-25-11" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "10 ng/mL FC std in Synth. Water" Annotation: ""

Sample Index: 80 Sample Type: QC Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 1:48:16 PM Modified: Yes Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 1594960 countsHeight: 2.58e+005 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 

0.0

1.0e42.0e43.0e44.0e45.0e46.0e47.0e48.0e49.0e41.0e51.1e51.2e51.3e51.4e51.5e51.6e51.7e51.8e51.9e52.0e52.1e52.2e52.3e52.4e52.5e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 40 of 41Printing Time: 2:35:03 PMPrinting Date: Monday, August 01, 2011
Sample Name: "k110728a081" Sample ID: "11 005-59" File: "k110728a.wiff"

Peak Name: "PFOA" Mass(es): "413.000/369.000 Da,413.000/219.000 Da,413.000/169.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 81 Sample Type: Unknown Concentration: N/A Calculated Conc: < 0 Acq. Date: 7/29/2011 Acq. Time: 2:09:26 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 22321 countsHeight: 4.56e+003 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 8 10 12 14 16 18

Time, min

0.0

1000.02000.03000.04000.05000.06000.07000.08000.09000.0

1.0e41.1e41.2e41.3e41.4e41.5e41.6e41.7e41.8e41.9e4

Intensity, cps

14.55

16.16

18.77

18.13

19.48

16.34

3.72 14.37 1.38 12.01

Sample Name: "k110728a081" Sample ID: "11 005-59" File: "k110728a.wiff"Peak Name: "13C8PFOA-IS(IS)" Mass(es): "421.000/376.000 Da"Comment: "Method Blank" Annotation: ""

Sample Index: 81 Sample Type: Unknown Concentration: 1.00 ng/mL Calculated Conc: N/A Acq. Date: 7/29/2011 Acq. Time: 2:09:26 PM Modified: No Proc. Algorithm: Specify Parameters - MQ III Noise Percentage: 50 Base. Sub. Window: 1.00 minPeak-Split. Factor: 2 Report Largest Peak: Yes Min. Peak Height: 0.00 cpsMin. Peak Width: 0.00 secSmoothing Width: 0 pointsRT Window: 30.0 secExpected RT: 15.8 minUse Relative RT: No Int. Type: Valley Retention Time: 15.8 minArea: 2360416 countsHeight: 4.01e+005 cpsStart Time: 15.7 minEnd Time: 15.9 min

0 2 4 6 

0.0

2.0e44.0e46.0e48.0e41.0e51.2e51.4e51.6e51.8e52.0e52.2e52.4e52.6e52.8e53.0e53.2e53.4e53.6e53.8e5

Intensity, cps

Workstation: ETSKIRK Results Name: k110728Data worked up by KJU Page 41 of 41Printing Time: 2:35:03 PMPrinting Date: Monday, August 01, 2011