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STUDY SPECIFIC SUPPLEMENT

To General Study Plan: 900-22

D-erythro-hex-2-enonic acid, .gamma.-lactone, monosodium 

salt [CAS RN 6381-77-7]:

Daphnia sp., 48-Hour Acute Immobilization Test

Study Director: D Sacker

Test Facility: Harlan Laboratories Ltd.

Shardlow Business Park

Shardlow

Derbyshire

DE72 2GD

UK

Sponsor: Univar USA Inc. 

17425 NE Union Hill Road 

Redmond 

WA 98052 

UNITED STATES OF AMERICA

Study Monitor: Greenwich Chemical Consulting, Inc.

3300 PGA Boulevard, Suite 635

Palm Beach Gardens

FL 33410

UNITED STATES OF AMERICA

Harlan Study Number: 41401174

Proposed Schedule:

Experimental Starting Date: 30 April 2014

Experimental Completion Date: 31 May 2014

Draft Report Date: 30 June 2014
Harlan Study Number: 41401174 Study Specific Supplement Page 2

Test Item

Information as provided by the Sponsor.

Identification: D-erythro-hex-2-enonic acid, .gamma.-lactone, monosodium salt 

[CAS RN 6381-77-7]

This study is being performed based on the U.S. Toxic Substances Control Act (TSCA) Section 

4 Test Rule and the regulations set forth under 40 CFR 799.5087.

Special Conditions

Study specific parameters:

GSP Section 1.1 Guidelines / Regulations: 

The study will be conducted according to the following internationally accepted guidelines and 

recommendations:

•The OECD Guidelines for Testing of Chemicals (2004) No 202 "Daphnia sp., Acute 

Immobilisation Test"

GSP Section 3.2 Test system

Daphnia used in the test should be from a healthy stock (i.e., showing no signs of stress such as 

high mortality, presence of males and ephippia, delay in the production of the first brood, 

discolored animals, etc.).

Any pH adjustments of the exposure solutions will be conducted according to section 13 of 

OECD 202 details of which will be included in the final test report.

GSP Section 3.8.2 Water quality criteria

Temperature, pH and dissolved oxygen measurements will be taken from replicate R1 only at 

the start and end of the test, and prior to and post test medium renewal if applicable. 

If measured concentrations are ± 20% of the nominal concentrations, results will be based on 

nominal concentrations. If initial measured concentrations are not ± 20% of the nominal 

concentrations but measured concentrations of the old exposure solution are ± 20% of the 

initial measured concentrations, results will be based on initial measured concentrations. If 

measured concentrations are not ± 20% of nominal concentrations, results will be based on 

geometric mean measured concentrations.

The study report will provide a detailed description of the analytical method that will be 

developed prior to and utilized during the test.
Harlan Study Number: 41401174 Study Specific Supplement Page 3

All elements described in item 27 of OECD 202 will be included in the final test report.

GENERAL INFORMATION

Distribution List

The original study specific supplement will be retained in the study file. Copies of the study 

specific supplement and general study plan along with any amendments will be distributed as 

follows:

For the Sponsor: John V. Massingale

Greenwich Chemical Consulting, Inc.

Quality Assurance: Harlan Laboratories Ltd, Shardlow, UK
Harlan Study Number: 41401174 Study Specific Supplement Page 4

SIGNATURES

Study Director: D Sacker

..................................................

Date:

Test Facility

Management: C Mead

..................................................

Date:

For the Sponsor: John V. Massingale

Greenwich Chemical Consulting, Inc.

.................................................

Date:

Note: These signatures also acknowledge the procedures described in the corresponding General 

Study Plan.