**Exhibit 8.1**

 

LIST OF SUBSIDIARIES OF CRUCELLN.V.

 

**List ofconsolidated companies**

 

TheCompany’s most significant subsidiaries as of December 31, 2008 were:

 

Legal seat2008 2007 2006

 
| ** **                    | ** ** | ** **          | ** ** | ** **      | ** ** | ** ** | ** ** | ** ** | **   Ownership** | ** ** | ** **                 | ** ** | ** ** |
|-------------------------------|------------|---------------------|------------|-----------------|------------|------------|------------|------------|---------------------------------|------------|----------------------------|------------|------------|
| **Name**                      | ** ** | **Legal seat** | ** ** | **Country**     | ** ** | ** ** | ** ** | ** ** | **   2008**      | ** ** | **   2007** | ** ** | **2006**   | ** ** |
| Crucell Holland B.V.          |       | Leiden              |       | the Netherlands |       |       |       |       | 100                             | %          |                       | 100        | %          |   | 100% |   |
| U-BiSys B.V.                  |       | Utrecht             |       | the Netherlands |       |       |       |       | 100                             | %          |                       | 100        | %          |   | 100% |   |
| ChromaGenics B.V.             |       | Amsterdam           |       | the Netherlands |       |       |       |       | 100                             | %          |                       | 100        | %          |   | 100% |   |
| Berna Biotech AG              |       | Bern                |       | Switzerland     |       |       |       |       | 100                             | %          |                       | 100        | %          |   | 100% |   |
| Berna Biotech  España SA |       | Madrid              |       | Spain           |       |       |       |       | 100                             | %          |                       | 100        | %          |   | 100% |   |
| Berna Biotech Italia Srl      |       | Milano              |       | Italy           |       |       |       |       | 100                             | %          |                       | 100        | %          |   | 100% |   |
| Etna Biotech Srl              |       | Catania             |       | Italy           |       |       |       |       | —                          |       |                       | 100        | %          |   | 100% |   |
| Berna Rhein B.V.              |       | Leiden              |       | the Netherlands |       |       |       |       | 100                             | %          |                       | 100        | %          |   | 100% |   |
| Rhein Vaccines B.V.           |       | Maastricht          |       | the Netherlands |       |       |       |       | —                          |       |                       | —     |       |   | 100% |   |
| Berna Biotech Korea corp.     |       | Seoul               |       | Korea           |       |       |       |       | 100                             | %          |                       | 100        | %          |   | 100% |   |
| Crucell Holding Inc.          |       | Wilmington, DE      |       | United States   |       |       |       |       | 100                             | %          |                       | 100        | %          |   | 100% |   |
| Crucell Vaccines Inc.         |       | Wilmington, DE      |       | United States   |       |       |       |       | 100                             | %          |                       | 100        | %          |   | 100% |   |
| Crucell Biologics Inc.        |       | Wilmington, DE      |       | United States   |       |       |       |       | 100                             | %          |                       | 100        | %          |   | 100% |   |
| SBL Vaccin Holding AB         |       | Stockholm           |       | Sweden          |       |       |       |       | —                          |       |                       | 100        | %          |   | 100% |   |
| SBL Vaccin AB                 |       | Stockholm           |       | Sweden          |       |       |       |       | 100                             | %          |                       | 100        | %          |   | 100% |   |
| Vitec AB                      |       | Stockholm           |       | Sweden          |       |       |       |       | —                          |       |                       | 100        | %          |   | 100% |   |
|                               |            |                     |            |                 |            |            |            |            |                                 |            |                            |            |            |  |  |  |  |