**Exhibit 8.1**

 

LIST OFSUBSIDIARIES OF CRUCELL N.V.

 

**Listof consolidated companies**

 

The Company’s mostsignificant subsidiaries as of December 31, 2009 were:

 
| **Name**                                            | ** ** | **Legal seat  ownership** | ** ** | **Country**     | ** ** | **2009  ownership** | ** ** | **2008  ownership** | ** ** | **2007  ownership** | ** ** |
|-----------------------------------------------------|------------|---------------------------|------------|-----------------|------------|---------------------|------------|---------------------|------------|---------------------|------------|
| Crucell Holland B.V.                                |       | Leiden                    |       | Netherlands     |       | 100%                |       | 100%                |       | 100%                |       |
| U-BiSys B.V.                                        |       | Utrecht                   |       | Netherlands     |       | 100%                |       | 100%                |       | 100%                |       |
| ChromaGenics B.V.                                   |       | Amsterdam                 |       | Netherlands     |       | 100%                |       | 100%                |       | 100%                |       |
| CrucellSwitzerlandAG(prior:BernaBiotechAG)          |       | Bern                      |       | Switzerland     |       | 100%                |       | 100%                |       | 100%                |       |
| CrucellSpainSA(prior:BernaBiotechEspañaSA)     |       | Madrid                    |       | Spain           |       | 100%                |       | 100%                |       | 100%                |       |
| Crucell Italy Srl (prior: Berna Biotech Italia Srl) |       | Milano                    |       | Italy           |       | 100%                |       | 100%                |       | 100%                |       |
| Etna Biotech Srl                                    |       | Catania                   |       | Italy           |       | —              |       | —              |       | 100%                |       |
| BernaRhein B.V.                                     |       | Leiden                    |       | Netherlands     |       | 100%                |       | 100%                |       | 100%                |       |
| Crucell UK Ltd.                                     |       | Shipley,  Bradford        |       | United  Kingdom |       | 100%                |       | —              |       | —              |       |
| Berna Biotech Korea corp.                           |       | Seoul                     |       | Korea           |       | 100%                |       | 100%                |       | 100%                |       |
| Crucell Holding Inc.                                |       | Wilmington,  DE           |       | United  States  |       | 100%                |       | 100%                |       | 100%                |       |
| Crucell Vaccines Inc.                               |       | Wilmington,  DE           |       | United  States  |       | 100%                |       | 100%                |       | 100%                |       |
| Crucell Biologics Inc.                              |       | Wilmington,  DE           |       | United  States  |       | 100%                |       | 100%                |       | 100%                |       |
| SBL Vaccin Holding AB                               |       | Stockholm                 |       | Sweden          |       | —              |       | —              |       | 100%                |       |
| Crucell  Sweden AB (prior: SBL Vaccin AB)           |       | Stockholm                 |       | Sweden          |       | 100%                |       | 100%                |       | 100%                |       |
| Vitec AB                                            |       | Stockholm                 |       | Sweden          |       | —              |       | —              |       | 100%                |       |