U N ITED STATE S ENVIRONMENTAL PROTECTlON AG E NC Y WASHI N GTON , O .C. 20460

. . OFFIC I:: OF 0 11:: : \I ICAL S AFETY

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## s:;- Aquabiotics Corporation 6075 NE Tolo Road Bainbridge Island , WA 98110 

## DEC 3 1 20J2 Attention : Mr. Stephan Paschal Subject
Purpose Label ing

Existing stocks Revisions.to Labeling 

### Fintrol® Fish Toxicant Kit EPA Reg. No . 39096-2 Addition of Webs ite Link to Standard Operating Procedure Revised Label of December 27 , 2012 You have agreed to revise your labeling to add l inks to your

.Standard Operating Procedure that the National Park Service

· hosts on its website . ·

The labeling attached to this letter, under the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA)**i~ ·**  

### acceptable . Per 40 c ·FR 156 . 1O(a)(6) , submit one copy ofyour fina l printed label i ng before you rel'ease the product for sh ipment. Follow the enclosed "Minimum Type Size Requirements for Final Printed Label" . .

.You may distribute or sell existing stocks of this product with current labeling for e ighte. en ( 18) months from the date of this

. letter.

If you wish to revise.the product labeling (outer container, two bottles, leaflet, or SOP) in the future , submit the revised :label ing to the Age ncy for review, including a list of the proposed changes , their location , and the reasons for such changes. Prior to distribution of the new label i ng , the Agency must approve any changes.

### Consequence for noncompliance Questions

Enclosure 2 

### If these conditions are not complied with, the registration will be subject to cancellation in accordance with FIFRA section 6(e). · Your release for shipment of the product constitutes acceptance

of.these conditions. Ifyou have questions about this letter, please contact Mr. Dan

. . Peacock at 703-305-5407 (phone) or peacock.dan@epa.gov (EMail).

1. Stamped Label ..- Sincerely, 

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# · · > &,M---./ '/ ':

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### .. .

/ Joh 1

### h Hebert . / Pr0duct Manager (07) 

# L**/~~ecticide-Rodenticide** "' Branch Registration Division (7505P) 2. Minimum Type Sizes for Final Printed LabelsFinfrol®**~h** Toxicant Kit . EPA Reg. No . 39096-2 December 27, 2012 

## ACCEPTED d: . 

## · DEC. 3 l 

# 2pF. Outer Can Label] 

## Underthe Federal Insecticide, Fungicide, . . I039096-oooo2.201212271 

## and 'Rodenticide Act, as amended, 

## for the [Front Panel] 

## pesticide re ist**~ ~**  

## - · EPA Reg No**~ESTRICTED** USE PESTICIDE [Type Size Requirement} · · Due to fox1c1ty 

### to flsN 

### atJCJ other aquatic organisms, the need for specialized training, acute oral toxicity, and eye irritation For retail sale to , and use only by, Certified Applicators or person under their direct supervision and only for those uses covered by the Certified Applicator's certification. FINTROL® FISH TOXICANT KIT (contains Fintrol® Concentrate, Fintrol Diluent, and Use Direction**Leaf~et)**

- . Ingredient Statements FINTROL® CONCENTRATE FINTROL® DILUENT Active Ingredients: Other Ingredients: Antimycin A .. .. .. .. .. .. .. . . 23% w/w Diethyl Phthalate Other Ingredients . Acetone .. .... .'................ · 77% w/w 100% 8 fl oz (bottle) . (surfactant)................ 30.5% w/w Nonoxynol -9- (det . ergent) 16.7% w/w Acetone .. .. .. .. .. .. .. .. .. .. .. .. 52.8% w/w 

### 1OO.Oo/o w/w 8 fl oz (bottle) ·

. KEEP OUT OF**~EACH** OF CHILDREN [Type Size Requirement] WARNING [Type Size Requirement] See Side Panels for additional precautionary statements. DIRECTIONS FOR USE IT IS A VIOLATION. OF FEDERAL LAW TO USE THIS PRODUCT IN A MANNER INCONSISTENT WITH ITS LABELING , INCLUDING THE CONTAINER LABEL AND SOP MANUAL [Standard**Oper~ting** Practices**fo~ th~**  Use of**Fintrol~** (Antimycin A) for

. . 

### . Restqration oJ. Native Fish Populations, National Park Service; USDi, November 2012] AVAILABLE ON-LINE [https ://i rma .n ps.gov/App/R .eferenceiProfile/2191334], AND.. FROM THE REGISTRANT, WITH ADDITIONAL-DIRECTIONS FOR USE . READ AND UNDERSTAND THE CONTAINER LABEL AND SOP PRIOR TO USE.

1Fintrol®. Fish Toxicant Kit EPA Reg . No. 39096-2 December 27 , 2012 · THE APPLICATOR IS RESPONSIBLE FOR FOLLOWING THE DIRECTIONS AVAILABLE FOR USE IN THE LABELING . See " USE DIRECTIONS LEAFLET' in this kit and the SOP for add itional use directions : [Side Panels] 

### FINTROL CONCENTRATE® PRECAUTIONARY STATEMENTS. HAZARDS TO HUMAN AND DOMESTIC ANIMALS WARNING. May be fatal if swallowed. Causes substantial but temporary eye injury. Do not get in eyes or on clothing . Wear protective eyewear (gqggles , face shield , or safety glasses), long -sleeved shirt, long pants , shoes , and socks . Wash thoroughly with soap and water after handling and before eating , drinking, chewing gum , using tobacco or using the . toilet. PERSONAL PROTECTIVE EQUIPMENT (PPE) Some materials that are chemical- resistant .to this product are butyl rubber, and to a lesser extent, polyethylene. If you want more options , follow the instructions for category "B' on an EPA chemical- resistance category selection chart . All mixers , loaders and other handlers exposed to the concentrate through clea ning equipment or spills must wear: • long-sleeved shirt and long pants , • chemica !-resistan. t gloves , • shoes plus socks , • protective eyewear (goggles , face shi. eld, or safety glasses) , . • a dust m ist filtering respirator (MSHA/NIOSH approval nu· mber prefix TC-21 G), or a ' NIOSH approved respirator with any N , R, P, or HE**filb~ r.**  and • a chemical-resistant apron . All applicators and other handlers must wear: • long-sleeved shirt and long pants , • chemical-resistant gloves , •**~hoes** plus socks , and • protective eyewear. In addition , applicators usi ng handheld equipment, handheld nozzles or other equipmentmust wear: • coveralls , and • a dust/mist filtering respirator (MSHA/NIOSH approval number prefix TC-21 C) ; or a NIOSH approved respirator with any N , R, P, or HE filter. 2Fintrol® Fish Toxicant Kit EPA Reg. No . 39096-2 December 27 , 2012 Exception : Applicators exposed to splashing water or walking in water that is being treated must wear waders instead of coveralls. All other applicators and handlers may substitute waders for long

_ pants if necessary.



### .User Safety Requirements Dq no wear contaCt lenses while handling this product. Ocular contact with this product can melt a contact lens onto**th~** eye , Follow manufacturer's instructions for cleaning/maintaining PPE : If no such instructions

.for washables , use detergent and hot water. Keep and wash PPE separately from other laundry. Remove PPE immediately**aft~r** handli ng this product. Discard clothing and other absorbent materials that have .been drenched or heavily · · contaminated with concentrated product. Do not reuse them . Wash thoroughly with soap and water after handling and before eating , drinking , chewing gum , using tobacco or using the toilet.. 

### User Safety Recommendations Certified Applicators applying or supervising any aspect of the application of this product should attend a training program for piscicide applications . · Users should wash hands before eating ,**~rinking ,**  chewing gum , using tobacco , or using the toilet. · Users should remove clothing/PPE immediately if pesticide gets inside. Then wash thoroughly and put on clean clothing.

· Users should remove PPE immediately after handling this product. Wash the outside of gloves before removing . . As soon as possible , wash thoroughly and change into clean clothing.

3

### Fintrol® Fish Toxicant Kit EPA Reg . No. 39096-2 December 27 , 2012

FIRST AID HAVE LABEL WITH YOU WHEN SEEKING TREATMENT ADVICE (1-800-858-7378)" IF SWALLOWED: · 

### Call a Poison Control Center; doctor. or 1-800-858-7378 immediately for treatment advice or transport the person to the nearest hospital. Do not give any liquid to ·the patient. Do not administer anything by mouth . Do not**indue~** 

### vomiting unless told to do so by the poison control center or doctor. IF IN EYES: 

### Hold eye open and rinse slowly and gently with water for 15-20 minutes . Remove contact lenses , if present, after the first 5 minutes , then continue rinsing eye. Call a poison contro l center, doCtor, or 1-800-858-7378 immediately for treatment advice . TREATMENT FOR PET POISONING 

### If an imal consumes Fintrol® Concentrate or diluted product, call veterinarian at once . .' ENVIRONMENTAL HAZARDS 

### This product is extremely toxic·to fish and other aquatic organ isms . Do not contaminate water outside the treatment area 

### by cleaning of equipment or disposal of equipment . wash waters. Do not contaminate water outside the treatment area , food , or feed by storage or disposal. Do not discharge effluent containing this pesticide into sewage systems without notifying the sewage treatment plant authority (POTW) . PHYSICAL OR CHEMICAL HAZARDS Extremely flammable. 

### Keep awa from fire , s arks , and heated surfaces.

4Fintrol® Fish Toxicant Kit EPA Reg. No . 39096-2 December 27 , 2012

STORAGE AND DISPOSAL [Type Size Requirements] Do not contaminate water, food or feed by storage or disposal. PESTICIDE STORAGE: Store only in original container, in a cool , dry place inaccessible to children and pets . Keep containers closed and away from other chemicals. Fintrol Concentrate: Product is stable for minimum of 3 years when stored in unopened , original glass bottles . Product will thicken if stored at temperatures below , 65F. Before use , store overn ight above ?OF. · Fintrol Diluent: Before use , store overnight above ?OF. PESTICIDE DISPOSAL: Pesticide wastes are acutely hazardous . Improper disposal

· of ex· cess pesticide , spray mixture , or rinsate is a violation of Federal Law. · Ifthese wastes cannot be disposed of by use according to label instructions , contact your State Pesticide or Envi ronmental. Control Agency , or the Hazardous Waste representative at the nearest EPA Regional Office for guidance. CONTAINER HANDLING: Nonrefillable container. Do not reuse or refill this container. · [Metal**Cat:~** and Glass Bottles] Tri ple rinse container (or equivalent) promptly after emptying. Triple rinse rinse as follows : Fill container %full with water and**r~cap.** Shake for 10 seconds . Drain for 10 seconds after the flow begins to drip . Follow .. Pesticide D isposal instructions for rinsate disposal. Repeat procedure two more times. Offer for recycling if**ava~lable o~**  place in trash or i n a sanitary landfill. [Note: As stated in 40CFR156 . 140(a)(4), a batch code must be included on the label or package for contai ner and containment for nonrefillables .]

Aquabiotics Corp . 6075 NE Tolo Road Bainbridge Island , WA 98110 EPA Reg. No. 39096-2 EPA Est. No. 39096-WA-01

5Fintrol® Fish Toxicant Kit EPA Reg . No . 39096-2 December 27 , 2012 [2. Fintrol® Concentrate Label]

FINTROL® CONCENTRATE Use with FINTROL ® DILUENT Active Ingredients: Antimycin A . . ... . ... :.. . . . . 23% w/w Other Ingred ients Acetone . . . . . . . . . . . . . . . . . . 77% w/w 100% 8 fl oz (bottle)

WARNING KEEP OUT OF REACH OF CHILDREN See FINTROL® FISH TOXICANT KIT label ,**U~e** Direction .Leaflet , and Fintrol SOP for add itional precautions , u· se di rections , storage and d isposal instructions, and other requirements. Aquabiotics Corp.

. P .O . Box 10576

Bainbridge Island , WA 98110 EPA Reg . No . 39096-2 EPA Est. No . 39096-WA-01

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# ~)tv Fintrol® Fish Toxicant Kit EPA Reg . No : 39096-2 December 27 , 2012 

### [3. Fintrol® Diluent Label] FINTROL® DILUENT Use with FINTROL- ® CONCENTRATE Other Ingredients: Diethyl Phthalate

.(surfactant) ......... . . .. . .. 30 .5% w/w Nonoxynol-9- (detergent) 16.7% w/w Acetone ....... . .. ... : . . . . . . . ... 52 .8% w/w · 100.0% w/w

· 8 fl oz (bottle) WARNING KEEP OUT OF REACH OF CHILDREN . \

See FINTROL® FISH TOXICANT KIT label , Use Direction Leaflet , and Fintrol SOP for additional precautions , use directions , storage and dispo'sal instructions , . and other

. requirements . ·

Aquabiotics Corp. · P :o. Box 10576

Bainbridge Island , WA 98110 EPA Reg. No. 39096'-2 · EPA Est. No. 39096-WA-01

7

# ·c··**to/~o** Fintrol® Fish Toxicant Kit · EPA Reg. No. 39096-2 December 27 , 2012 [4. Fintrol ®Fish Toxicant "Use Directions Leaflet"] FINTROL® FISH TOXICANT USE DIRECTIONS LEAFLET DIRECTIONS FOR USE IT IS A VIOLATION. OF FEDERAL LAW TO USE THIS PRODUCT IN A MANNER I NCONSISTENT WITH ITS LABELING , INCLUDING THE CONTAINER LABEL AND

. . . . SOP MANUAL

### [Standard**Op~r9tirig** Practices for the Use of Fintrol® (Antimycin 

### A) for

### Re~torat'ion pf Native Fish

### Popul~tions**,** Nation. ai'Park

### Servi~e• . l)SDI;.November 2012] AVAiLABLE : ON - LINE [https ://irma .rips .gov/App/Refererice/P.rofile/2191334], AND

. FROM THE REGISTRANT· , Wl'iH ·ADDITIONAL DIRE. CTIONS FOR USE. READ AND UNDERSTAND THE CONTAINER LABEL AND SOP PRIOR TO USE. THE APPLICATOR IS RESPONSIBLE FQ.R FOLLOWING THE DIRECTIONS · AVAILABLE FOR USE IN THE LABELING. General Applicati<?n Restrictions . for all Formulations . The Certified Applicator supervising the treatment must remain on-site for the duration of the application . ·

. Do not allow recreational access (e .g ., wading , swimming, boating, fishing) within the**tre~tment** area while antimycin A is being applied and .for 

### 7 days after treatment.

For "complete.kill'! use , do not apply this product in a way that wi ll result in treatment conc!3ntrations greater than 25 parts per billion . For 'selective kill' use in aquaculture, do not apply this product in a way that will result in treatment concentrations greater . than 10 parts per billion. Do not apply this product in a way that will contact workers or other persons, either directly or through drift. Only protected handlers may be in the area during application .

· Do not apply with any application method or equipment or in a manner not specified on this label or in the antimycin A SOP Manual. This product must not be applied to estuarine or marine environments. Where practical , users should collect and bury dead fish. 8.Fintrol® Fish Toxicant Kit EPA Reg . No . 39096-2 December 27 , 2012 Additional Requirements for Use in Aquaculture When antimycin A is applied as ·a selective kill in aquaculture , the Certified Applicator supervising the applicatiqn must inform the owner /operator of the aquaculture site being treated that surviving fish must not be .harvested for food or feed for a minimum of 12 months after treatment. When antimycin A is ·ap.plied as a complete**~ill** in aquaculture , the Certified Applicator supervising the application must inform the owner /operator of the aquaculture site being treated that the water body must not be restocked with fish for a minimum of 7 days after treatment, OR once 3 co. nsecutive monitoring samples taken no less than 4 hours apart demonstrate that antimycin A levels are below the limit of detection (0.015 ppb). Drinking Water Notification

·Ifdrinking water intakes are present within the treatment area , or a hydrologic connection to wells exist, 7-14 days prior to application , the Certified Applicator o r designee under his/her direct supervision niust provide notification to the party responsible for the public water supply or to individual private water·users. Drinking water intakes within the treatment area must be closed during treatment and until 3

. consecutive mon itoring samples taken no less than 4 hours apart demonstrate that

.antimycin A levels are below the limit of detection (0 .015 ppb) . Deta iled instructions for public involvement, notifications , and monito r:ing are presented in . the antimycin A SOP Manual.



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# c· c .· t2-} 2.0 Fintrol® Fish Toxicant Kit EPA Reg . No. 39096-2 

### December 27, 2012· Placarding of Treatment Areas, Except Aquaculture Applications 

### The Certified Applicator in charge ofthe application (or someone under his/her supervision) must placard all access areas to the treatment area. Detailed instructions for placarding are presented in the Antimycin A $0P Manual. Placards must be placed every 250 

### feet along the shoreline ofthe treated area , OR , at pub lic access points (e .g., trailheads and roads and trails) . Placards must contain the information below: • .DANGER/PELIGRO • DO NOT ENTER WATER/NO ENTRE : Pesticide Application

• . [The name of the product applied] • [The name , address , and te lephone number of the responsible agency or entity performing the application] • [The purpose of the appl ication] • [The start date and time of application] • [The end date and time of application] • [The duration of the area closure] • Recreational access (e .g ., wad i ng , swimming , boating , fishing, etc.) within the**treatm~nt** 

### area is prohibited while antimycin A is being app lied and for 7 days after treatment. • Do not swim or wade in treated water. • Do not consume water or dead fish from treated water. S igns must remain leg ible during the entire posting period and must be removed no earlier than 7 days after treatment and no later than 14 days after treatment. · Mechanism of Action 

### The active ingredient of FINTROL is antimycin A. 

### When absorbed through the gills of fish , antimycin A kills by interfering with the respiration of body·cells. Antimycin A does not repel. fish . This is an important"advant;:1ge, particularly when running waters, bo· g lakes , and the epilimnion , or upper layer of large lakes are treated. Fish make no attempt to escape contact with the toxicant by seeking to move into waters that are clear of it. FINTROL'S action is rapid arid Irreversible .

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# ( Fintrol® Fish Toxicant Kit EPA Reg. No. 39096-2 December 27, 2012 Sensitivity to FINTROL varies widely among fish species . Hence it may be employed to selectively destroy certain species , without affecting other species concurrently inhabiting the same body of water. Sensitive:

. . 

### '

.Gizza. rd shad , trouts , pikes , carp, minnows , sucl<ers , brook stickleback , white . bass , sunfishes , perches , freshwater drum , sculpins . · · Least Sensitive: 

### Shortnose gar, bowfin , goldfish , catfish . · FINTROL also may be used to selectively destroy certain age groups of species; younger fish are more sensitive to FINTROL. Providing the concentration is correctly estimated , FINTROL can be used effectively at

· any time of year in·either cold , warm , soft, hard , acid , alkaline , clear or tu rbid '(muddy) waters. (See TABLE 1 and instruction for bioassay.) FINTROL does not impart detectable taste or odor to treated waters . In the usual , recommended concentrations , it**cause~** 

### no apparent harm to aquatic plants, insects , or bottom fauna. Since FINTROL' S active ingredient degrades rap idly, the reclaimed waters may be restocked soon afte r treatment. (See HOW TO DETERMINE WHEN TREATED WATER MAY BE RESTOCKED.) There is very little interruption in availability of the waters for recreational , agricultural , industrial , or other purpose . Complete and Selective Kills 

### This product may be used to achieve a 'complete kill' or a 'selective kill .' Complete kills are intended to eliminate all fish in the treatment area whereas selective kills , used only in aquaculture , are intended to eliminate or reduce the number of only certain (more vulnerable) species . Detailed instructions for conducting complete and selective kills · are presented in the antimycin A SOP Manual and are summarized below.

A complete kill may be achieved with a concentration of anywhere from 

### 5 to 25 p.p· .b. of active ingredient (See HOW TO DETERMINE THE MOST EFFECTIVE CONCENTRATION.) FINTROL is particularly advantageous for complete kills because if detoxifies so rapidly the pond can usually be restocked in about a week , or as soon as caged fish survive 48 hours' exposure to the treated waters. Under optimal circumstaf"lces , in ponds managed for sports fishing , selective kills may be achieved at concentrations as low as 0.5 to 1 .0 p.p .b. However, because these concentrations are extremely low , there is no rule of thumb that can be relied liPOn to determine them accurately. A BIOASSAY IS ALWAYS REQUIRED TO PINPOINT THE OPTIMAL CONCENTRATION FOR SELECTIVE KILLS. 11

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## t.O Fintrol® Fish Toxicant Kit EPA Reg . No . 39096-2 December 27 , 2012 A selective kill has these advantages : It can be made without i nterrupting sport fishing for ·more than a week or so , and fishing may be gradually improved without restocking. In the past, when bluegill, minnows , or green sunfish dominated a pond managed for bass , the usual solution to the problem was the total removal of all the fish with a fish toxicant This meant restocking and little or no f ish i ng for one or two years. Now--- with FINT R 0 L --- this is no longer necessary. Low concentrations of FINTROL will affect small bluegill , green sunfish , and minnows primarily. Only a few of the very small bass will succumb. The bulk of the adult bluegill and green sunfish will not be affected . Thus FINTROL helps to bring about a ba lanced relationship between the bass and bluegill populations . This improves fish i ng without interrupting it for any appreciable length of time of time . · In catfish farming FINTROL can be used to selectively eliminate the trash fish (scale fish) that commonly redu . ce the y.ields and increase the costs of the commercial . catfish farmer. It ispossible to do this with FINTROL because concentrations that will elim inate scale fish generally will not harm adult catfish . The scale fish most often encountered by the catfish farmer will succumb to anywhere from 5 to 10 p .p .b. of active ingredient (See TABLE 1) whereas , under ordinary circumstances ,· it takes in excess of 20 p .p.b. to kill catfish. (Caution should be exercised .during stress cond itions of unusually high water temperature and reduced o)(ygen content when the sensitivity of fishes to chemical may increase .) .I

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# ( Fintrol® Fish Toxicant Kit EPA Reg . No. 39096-2 December27 , 2012 · 

### How to Determine the Most Effective Concentration

. . The nature of the water to be treated (its depth) and rate of flow) and the character of the surrounding land are factors to be taken into consideration when determining the formulation of FINTROL to. employ in 

# agiven situation. 

### 1. Complete kills The concentration of. antimycin A required to kill one or more species of fish in any given body of water depends upon: I) the sensitivity· of the species to be eradicated , and 2) the chemical and physical properties of the water at the time of application of the·toxic . ant; the pH and the temperature of the water are the most important of these · · chemical and physical factors .under ord i nary circumstances. Therefore , to determine what concentration of antimycin A will be required to kiil the undesirable fish in your pond or lake: · 1) identify the species to be eradicated , 2) determine the pH and average water temperature by measuring at various sites and depths ,

. 3) refer to TABLE 1 for approximate concentrations. 4) conduct a bioassay to pinpoint the optimal concentration. TABLE 1 provides a ·rough estimate of the concentrations required for a complete kill under various environmental conditions . However, since water chemistry is subject to sudden alteration by many variables and often unpredictable factors (pollution , heavy algae bloom , weather, drawdown, etc.) it should be realized that such changes may affect the performance of the toxicant. For this reason , · measurements of pH and water temperature**sho~:Jid** always be taken as close to the

time of treatment as is feasible .

13 .·.Fintrol® Fish Toxicant Kit EPA Reg. No. 39096-2 December 27 , 2012

Table 1. Estimate ofAntimycin A concentrations needed for complete kill offish species at different water temperature and pH ranges. Target Sensitivity Estimated Concentration (ppb) ofAntimycin A Species 1 (ppb) for Complete Kill If ph is 8.5 or Less If pH is More than 8.5 Water Water Water Water Temperature Temperature Temperature Temperature

Above 60F Below60F Above 60F Below 60F

.gizzard shad trouts pikes carp minnows suckers ' brook ·

stickleback 5-10 5 7.5 7.5 10 white bass sunfishes perches**freshwa~er**

drum sculpins short nose gar 15-25 15 20 20 25 boWfin goldfish catfish

Notes 1. Fish nomenclature according to American Fisheries Society 2. 

### 1re concentratiQ111evel**SL~f:QE!Sted** 

### bythis tci:le strud 

### be 

### cx:nfinTed byen 

### msite bioassay. . 3. Ths**~e** is appic:;aje only a complete kill is desired. Do not use it for a selective kill. (See the following section.)

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# c -**t-1-J~o** Fintrol® Fish Toxicant Kit EPA Reg. No. 39096-2 . Oecember·27, 2012 · The sensitivity of the target species determines the concentration range. To eradicate sens itive species, it is recommended thatthe appropriate formulation at FINTROL be applied so that the body of water will have a concentration of from 5 to 10 p.p.b. of antimycin A; depending upon variation on i n pH and water temperature. For more tolerant species, higher concentrations are recommended . Laboratory Studies indicate that less sensitive fish will succumb at concentrations of from .15 to 25 p.p_b of antimycin A , depending upon variations in pH and water temperature . Columns 3 to 6 show how to adjust for pH and water temperature_ Note that, in general , the lower · the pH, the less FINTROL required. The higher the water temperature , the less FINTROL required. The ideal situation for a complete kill would combine a highly sensitive species , low pH and ·high water temperature . 

### 2. Selective Kills The only way to determine the concentration of FINTROL needed for a selective kill is to perform 

# a bioassay. This involves subjectin. g both the target and nontarget fish to several concentrations of FINTROL to determine the minimum lethal dose. (A description of the bioassay procedure is available upon request.)

How to Calculate Amount of Fentrol to be Added to a .Body of Water to Obtain a . Given Concentration of Antimycin A To calculate the amount of FINTROL to be added to a body of water for eradication of undesired species , the following steps should be taken: 1 . Determine the·volume of water to be treated in acre-feet. This can be arrived at bymultiplying the surface area in acres by the average depth in feet. 2. Determine the concentration to be used from Table I. 3. Multiply the number of acre-feet by the value given in Table 2 , opposite the desired concentration. 4 . The value obtained for volume may be divided by the total kit vol ume (480 cc. or 16 oz.) to get number of Fish Toxicant Kits needed .

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### ( Fintrol® Fish Toxicant Kit EPA Reg . No. 39096-2 December 27, 2012 Table 2 , Rapid estimate of amount of .FINTROL® Concentrate needed (per acre-foot) to produce desired concentration of Antimycin ·A (in ppb). ' \ Desired Concentration of Amount of Fentrol® Concentrate Needed Antimycin A (ppb) (per acre-foot) cc1 oz . 1 12 .3 Y2 

### 2 24.6 % 

### 3 36 .9 1% 

### 4 49.2 1 Y2 

### 5 61.5 2 

### 6 73.8 2Y2 

### 7 86.1 2% 

### 8 98 .4 3 - % 

### 9 110.7 3% 10 123.0 4 1obtained by multipiying 12.3 cc by·the ppb . 

### Sample calculation: · To treat 75 acre-feet at 3 p .p .b 

### Using 

### cc·measurements: 75 x 36.9 cc 

### =2,767 cc. of FINTROL-CONCE.NTRATE 

### Using ounce measurements: 75 X 1**~fl.** OZ. = 93% fl. oz. of FINTROL-CONCENTRATE

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# "o Fintrol® Fish Toxicant Kit EPA Reg. No. 39096-2 December 27, 2012 

### Methods of Application IMPORTANT: Review guidelines in these instructions for use of Pers· onal Protective · Equi pment {PPE)

Liquid formulation: Directions for mixing: Add the Diluent (blue label] to the FINTROL CONCENTRATE (solution 23%) [Green label] in the oversize mixing container. Cap tightly and invert 2 to 3 times to mix thoroughly. Further. dilute with AT LEAST five (5) gallons ofwater to insure that the aceto11e contained in FINTROL-CONCENTRATE will not affect rubber parts on any equipment that might be used to apply it. After water has been added, apply within eight (8) hours . (Note : The solution obtained by mixing the Diluent with FINTROL-CONCENTRATE (solution 23%) retains potency for up to seven (7) days. But once water has been added to this solution , it must be used within eight (8) hours to ensure potency.] After appropriate dilution with water, the liquid formulation of FINTROL can be applied to lakes and ponds by the boat bailer method or'spray equipment. Spray methods are useful at depths to one foot. · Boat bailer and drip tubes when applied at the propeller wash are useful at greater depths . Pinpoint applications to shoal areas and small , isolated ponds can readily be made with backpack sprayers. (See CAUTION on use of PROTECTIVE GOGGLES AND PROTECTIVE GLOVES .) In streams, FINTROL-CONCENTRATE is most often applied through drip stations established to meter the toxicant at a precalculated rate. Information on the use of such equipment m· ay be obtained from state and/or.federal agencies , experienced in strearn treatment. [t is recommended that all applications of FINTROL be made at daybreak or as soon as there is enough light to work by. 

### Potency of Mixed 'Formulations Leftover portions of mixed liquid formulation retain potency for up to seven (7) days . But once water has been added to FINTROL-CONCENTRATE , it must be used within eight (8) hours toensure potency.**Ho~** 

### to Determine When Treated Water May be Restocked

. . Since antimycin A degrades rapidly following application , waters can usually be restocked about one week following treatment with FINTROL. Place l ive cars containing a 

### sensitive species of fish in the treated water. Use fingerling rainbow trout or fingerling bluegills if the water temperature is between 35 and 68F . When the water temperature exceeds 68F , use only fingerling bluegills. If the fish survive for 48 hours,

. the water may be restocked . 

### Deactivation With Potassium Permanganate (KMn04 ) 17

# c· ( Fintrol® Fish Toxicant Kit EPA Reg . No . 39096-2 December 27, 2012 Flow in a stream and outflow from a treated lake beyond the treatment area must be deactivated with potassium permanganate to minimize exposure beyond the treatment area unless unnecessary . (See the AntimyCin A SOP Manual for the definition of treatment area , examples when treatment with potassium permanganate is**unnec~ssary** 

### and detailed ·guidance for deactivating antimycin A with potassium permanganate,) · To prevent killing fish downstream, apply potassium permanganate at 1 part per million (1 p.p.m .) to the outflow . (More potassium permanganate may be needed if the stream has a high permanganate demand . Drip systems of hose-and-clamp or carburetor types can be employed to continuously dispense a solution of potassium ·permanganate into the water at the discharge outlet. · To evaluate the effectiveness of the detoxification process, place livecars contain ing f ingerling rainbow trout or fingerling bluegills approximately 100 yards downstream · from the site of KM 

## n 0 4 introduction . The water is considered detoxified if the fish survive for at least 48 hours in the livecar. · To detoxify FTNTROL-treated streams , apply KMn04 at 1 p.p.m . at detoxification stations . (More K.Mn04 may be needed ifthe stream has a high permanganate demand) . Continue the application KMn04 until all FINTROL-treated water has passed the station . The water may be considered detoxified when fingerling rainbow trout or fingerling bluegills survive for at least 48 hours in livecars place 100 yards ·. · downstream from the site of potassium permanganate (KM n04) introduction . Re-entering The Treatme· nt Area 

### For the first 7 days after treatment, handlers re -entering treated**w~ter m~st**  

### wear, at a min imum, the following PPE : • Waders and long-sleeved shirt, • chemical-resistant g loves , and • protective eyewear. Special Instructions Regarding State Government Regulatory Agencies 

### Prior to the use of a fish toxicant in either public or private waters , the Director of the State Fish aod Game Department or Conservation Department must be contacted to determine yvhether a permit is required . .Such products must be used by or under the technical supervision where required , of personnel of state and federal fish and game agencies , .trained in fisheries management, who wi ll provide any special instructions applicable to the particular geographical area . · ·

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