Office of Water

Office Science and

Technology

EPA 820-D-14-021 

May 2014

Draft Update of Human Health 

Ambient Water Quality Criteria:

Bromoform

75-25-2 

Br

Br

Br
EPA 820-D-14-021

May 2014

Update of Human Health 

Ambient Water Quality Criteria:

Bromoform

75-25-2 

Draft

Office of Science and Technology

Office of Water

U.S. Environmental Protection Agency

Washington, DC 20460
Bromoform 75-25-2 

Table of Contents

Introduction: Plan and Scope of Update

Problem Formulation

Criteria Formulas- Analysis Plan

Exposure Factors

Body Weight

Drinking Water Intake

Fish Consumption Rate

Bioaccumulation Factor

Hazard Identification and Dose Response: Cancer Slope Factor

Criteria Derivation- Analysis

Criteria Characterization

Chemical Name / Synonyms

References

Introduction: Plan and Scope of Update

Human health ambient water quality criteria (AWQC) are numeric values for pollutant 

concentrations in ambient waters that the U.S. Environmental Protection Agency (EPA) 

considers to be protective of human health. EPA periodically revises water quality criteria to 

ensure that they reflect the latest scientific knowledge. The current revisions of the criteria for 

bromoform, contained in this document, incorporate updated information regarding cancer 

risk, body weight, drinking water intake, fish consumption rate, and bioaccumulation. Updated 

cancer risk information for bromoform is identified in EPA’s Office of Water 2005 toxicology 

assessment (USEPA, 2005). Updated body weight and drinking water intake data are identified 

in EPA’s ‘Exposure Factors Handbook: 2011 Edition’ (USEPA, 2011). The bioaccumulation factor 

data is updated using EPA’s Estimation Program Interface (EPI) Suite modeling program (USEPA, 

2012a). The overall fish consumption rate and trophic level breakdowns are updated using 

EPA’s ‘Estimated Fish Consumption Rates for the U.S. Population and Selected Subpopulations 

(NHANES 2003-2010)’ (USEPA, 2014). Unless otherwise specified, all sources of information 

used in this update are from peer reviewed sources.

Note that the term "water quality criteria" can carry one of two possible meanings, 

depending on the section of the Clean Water Act (CWA) associated with the particular criteria 

at issue. “Water quality criteria” may refer to national water quality criteria recommendations 

issued under CWA § 304. “Water quality criteria” may also refer to water quality criteria 

components of water quality standards adopted by states, territories, or authorized tribes 

under CWA § 303.

National ambient water quality criteria recommendations for human health are issued 

by the EPA under CWA §304. They are based on the latest scientific information on the 

relationship between the effects of a constituent concentration and human health. Protective 

assumptions are made regarding the potential human exposure intakes. These criteria do not 

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Bromoform 75-25-2 

reflect consideration of non-human health endpoints or economic impacts. Nor do they reflect 

the technological feasibility of meeting the chemical concentrations in ambient water. National 

ambient water quality criteria recommendations for human health are not automatically 

incorporated into water quality standards adopted by states, territories, or authorized tribes. 

Rather, they provide scientific information to states, territories and authorized tribes to assist 

them in adopting ambient water quality criteria for human health in water quality standards 

that meet CWA requirements. National ambient water quality criteria recommendations for 

human health are not regulations themselves and they do not impose legally binding 

requirements on EPA, states, territories, authorized tribes, or the public. States, territories, and 

authorized tribes have the discretion to adopt, where appropriate, other scientifically 

defensible water quality criteria that differ from EPA’s national ambient recommended criteria 

for human health.

Ambient water quality criteria components of water quality standards are generally 

adopted by the states, territories, and authorized tribes themselves, under §303(c)(2). (In 

certain circumstances EPA also may promulgate this type of criterion itself, pursuant to 

§303(c)(4)). State ambient water quality criteria for human health represent a quality of water

that sufficiently protects human health to support a designated use of the state, territory, or 

authorized tribe. Such criteria may be expressed in terms of constituent concentrations, levels, 

or narrative statements. Once ambient water quality criteria for human health are adopted by a 

state, territory, or authorized tribe into their water quality standards, they provide a basis for 

controlling discharges or releases of pollutants, for developing permit limits, assessing waters, 

and developing total maximum daily loads (TMDLs) for waters that do not meet the water 

quality standard. Ambient water quality criteria for human health have a regulatory impact 

once they have been adopted into water quality standards by the state, territory, or authorized 

tribe under § 303(c)(2) (or alternatively issued by EPA pursuant to § 303(c)(4)).

The water quality criteria at issue in this document are national ambient water quality 

criteria recommendations for human health issued under CWA § 304. Unless expressly 

indicated otherwise, all references to “criteria,” “water quality criteria,” “ambient water quality 

criteria (AWQC) recommendations,” or similar variants thereof, are references to national 

ambient water quality criteria recommendations for human health.

Problem Formulation

Problem formulation provides a strategic framework for water quality criteria 

development by focusing on the most relevant endpoints and increasing the transparency of 

the effects assessment process. The structure of this criteria document is consistent with U.S. 

EPA’s ‘Framework for Human Health Risk Assessment to Inform Decision Making’ (USEPA, 

2012b).

In the development of AWQC, EPA currently follows the deterministic assessment 

methodology outlined in EPA’s ‘Methodology for Deriving Ambient Water Quality Criteria for 

the Protection of Human Health (2000)’ (USEPA, 2000), hereafter known as the 2000 

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Methodology. For the development of criteria for carcinogens that express a non-threshold, 

linear dose response, the 2000 Methodology takes into consideration exposure factors (body 

weight, drinking water intake, fish consumption, and bioaccumulation), the increased cancer 

risk due to exposure to the pollutant, and a 10-6 (or 1 in 1,000,000) risk level for the general 

population. The 10-6 risk level utilized in the derivation of the AWQC represents the water 

concentration that would be expected to increase an individual’s lifetime cancer risk from 

exposure to the particular pollutant by no more than one chance in one million, regardless of 

the additional lifetime cancer risk due to exposure, if any, to that particular substance from 

other sources. States and authorized tribes may consider adjusting exposure assumptions and 

related model inputs according to guidance in the 2000 Methodology, to assure that 

subpopulations are adequately protected if data are available. 

Criteria Formulas- Analysis Plan

The following formulas are used to develop EPA’s CWA Section 304(a) human health 

ambient water quality criteria. EPA develops criteria for ambient waters typically considering

two routes of exposure. The first formula can be used to derive a human health criterion that 

assumes exposure through both the consumption of water and the consumption of aquatic 

organisms. The second formula can be used to derive a criterion that assumes exposure 

through the consumption of aquatic organisms, but not water. The use of one criterion over the 

other depends on the designated use of the water bodies in question (i.e. drinking water source 

and/or fishable waters). 

EPA recommends inclusion of the drinking water exposure pathway for ambient surface 

waters where drinking water is a designated use for the following reasons: (1) Drinking water is 

a designated use for surface waters under the CWA, and therefore, criteria are needed to 

assure that this designated use can be protected and maintained. (2) Although rare, there are 

some public water supplies that provide drinking water from surface water sources without 

treatment. (3) Even among the majority of water supplies that do treat surface waters, existing 

treatments may not necessarily be effective for reducing levels of particular contaminants. (4) 

In consideration of the Agency’s goals of pollution prevention, ambient waters should not be 

contaminated to a level where the burden of achieving health objectives is shifted away from 

those responsible for pollutant discharges and placed on downstream users to bear the costs of 

upgraded or supplemental water treatment (USEPA, 2000).

EPA recommends the organism only criterion in those cases where the designated uses 

of a water body include only supporting fishable uses under Section 101(a) of the CWA, and

thus, fish or shellfish for human consumption, but not as a drinking water supply source (e.g., 

non-potable estuarine waters) (USEPA, 2000).

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The formulas for deriving the criteria values are as follows (USEPA, 2000):

For consumption of water and organisms:

 AWQC (μg/L) = [10-6* / CSF (kg·d/mg)] × BW (kg) × 1000 (μg/mg)† 

 DI (L/d) + ∑ (FCRi (kg/d) × BAFi (L/kg)) 4

i=2

 (Eq. 1)

For consumption of organisms only:

 AWQC (μg/L) = [10-6* / CSF (kg·d/mg)] × BW (kg) × 1000 (μg/mg)† 

∑ (FCRi (kg/d) × BAFi (L/kg)) 4

i=2

 (Eq. 2)

Where:

AWQC = ambient water quality criteria (at the 10-6 risk level)

CSF = cancer slope factor

BW = body weight

DI = drinking water intake

∑ 4

i=2 = summation of values for aquatic trophic levels (TL) where the letter “i” stands for the 

trophic levels to be considered, starting with TL 2 and proceeding to TL 4

FCRi = fish consumption rate for aquatic trophic levels 2, 3, and 4

BAFi = bioaccumulation factor for aquatic trophic levels 2, 3, and 4

The following sections identify each of the components of the formulas for the human 

health ambient water quality criteria and provide reference sources, previously used values 

when available, and the values to be used in the updated criteria.

Exposure Factors:

Body Weight

The updated recommended body weight (BW) is 80 kg which represents the mean 

weight for adults 21 years of age and older. This recommendation is found in EPA’s ‘Exposure 

Factors Handbook: 2011 Edition’ in Chapter 8. It was based on data derived from the National 

Health and Nutrition Examination Survey (NHANES) 1999−2006 (USEPA, 2011). This body 

weight replaces the recommended standard weight for adults of 70 kg that was described in 

the 2000 Methodology which was approximated from the mean body weight of adults from the 

National Health and Nutrition Examination Survey (NHANES) III database (1988-1994) and a 

1989 study by the National Cancer Institute (USEPA, 2000). 

* 10-6 or 1 in 1,000,000 risk level for the general population † 1000 μg/mg is used to convert the units of mass into micrograms from milligrams.

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Bromoform 75-25-2 

Drinking Water Intake

The updated drinking water intake (DI) is 3 L/day, rounded from 3.091 L/day for 

consumer-only estimates of direct and indirect water ingestion based on NHANES 2003-2006 

data for all sources of water at the 90th percentile for adults (≥21 years of age) (USEPA, 2011). 

Direct water is defined as water ingested directly as a beverage (from all sources); indirect 

water is defined as water added in the preparation of food or beverages, not including indirect 

consumption of bottled water. This recommended value is found in EPA’s ‘Exposure Factors 

Handbook: 2011 Edition’ in Table 3-36. It replaces the drinking water intake standard of 2 L/day 

described in the 2000 Methodology which represented the 86th percentile for adults 20 years 

and older in the US Department of Agriculture’s 1994-96 Continuing Survey of Food Intake by 

Individuals (CSFII) analysis or the 88th percentile of adults in the National Cancer Institute study 

of the 1977-78 Nationwide Food Consumption Survey (USEPA, 2000).

Fish Consumption Rate 

The updated fish consumption rate (FCR) for the general adult population is 22 

grams/day (0.022 kg/day) (USEPA, 2014: see Table 9a). It represents the 90th percentile per 

capita consumption rate of freshwater and estuarine fish for the U.S. adult population 21 years 

of age and older based on the Centers for Disease Control and Prevention (CDC) National 

Health and Nutrition Examination Surveys (NHANES) conducted from 2003-2010. It replaces the 

FCR default of 17.5 grams/day, which represented an estimate of the 90th percentile per capita 

consumption rate of freshwater and estuarine fish for the U.S. adult population based on the 

U.S. Department of Agriculture’s (USDA’s) Continuing Survey of Food Intake by Individuals 

(CSFII) 1994-96 data (USEPA, 2002a). 

As recommended in the 2000 Methodology, this update to the criteria distinguishes a 

trophic level (TL) breakdown of the fish consumption rate to provide a better representation of 

dietary exposure to fish at multiple trophic levels. An organism’s trophic position in the aquatic 

food web can have an important effect on the magnitude of bioaccumulation of certain 

chemicals. The trophic levels are numbered 2, 3, and 4 and account for different categories of 

fish and shellfish species based on their position in the aquatic food web. TL2 accounts for 

benthic filter feeders, TL3 accounts for forage fish, and TL4 accounts for predatory fish. 

In order to derive the trophic level breakdown of the 22 grams/day freshwater and 

estuarine FCR, the trophic level specific freshwater and estuarine FCR data sets for adults 21 

years of age and older were identified (USEPA, 2014: see Tables 16a, 17a, and 18a). In each 

trophic level specific data set, the ratio of that trophic level’s 90th percentile FCR compared to 

the summation of all three trophic level specific 90th percentile FCRs was applied to the 22 

grams/day FCR used in this update. The trophic level ratios were calculated as follows: TL ratio 

= 90th percentile FCR for the TL divided by the sum of the 90th percentile FCRs for all TLs. TL2 = 

7.6/21.3 = 0.3568 (35.68%), TL3 = 8.6/21.3 = 0.4038 (40.38%), and TL4 = 5.1/21.3 = 0.2394 

(23.94%). Applying these ratios to the updated FCR of 22 grams/day result in trophic level 

breakdowns of TL2 = 8 grams/day (0.008 kg/day); TL3 = 9 grams/day (0.009 kg/day); and TL4 = 5 

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Bromoform 75-25-2 

grams/day (0.005 kg/day). These resulting trophic level FCRs are well within the confidence 

intervals for each of the trophic level specific distributions’ 90th percentiles and add to the total 

of 22 grams/day (USEPA, 2014).

Bioaccumulation Factor

Several attributes of the bioaccumulation process are important to understand when

deriving national BAFs for use in setting national 304(a) criteria. First, the term

“bioaccumulation” refers to the uptake and retention of a chemical by an aquatic organism 

from all surrounding media (e.g., water, food, sediment). The term “bioconcentration” refers to 

the uptake and retention of a chemical by an aquatic organism from water only. For some 

chemicals (particularly those that are highly persistent and hydrophobic), the magnitude of 

bioaccumulation by aquatic organisms can be substantially greater than the magnitude of 

bioconcentration. Thus, an assessment of bioconcentration alone may underestimate the 

extent of accumulation in aquatic biota for these chemicals. Accordingly, EPA’s guidelines 

presented in the 2000 Methodology emphasize the measurement or estimation of chemical 

bioaccumulation by aquatic organisms (USEPA, 2000). 

The bioaccumulation factors (BAF) have been estimated using EPA’s Estimation Program 

Interface (EPI) Suite (USEPA, 2012a). The BCFBAFTM program within EPI Suite estimates fish 

bioaccumulation factors using octanol-water partition coefficients (KOW) and biotransformation 

data from a model designed by Arnot and Gobas (2003). The model includes mechanistic 

processes for bioaccumulation such as chemical uptake from the water at the gill surface and 

from the diet, chemical elimination at the gill surface, fecal egestion, growth dilution and 

metabolic biotransformation. Other processes included in the calculations are bioavailability in 

the water column (only the freely dissolved fraction can bioconcentrate) and absorption 

efficiencies at the gill and in the gastrointestinal tract. The model requires the octanol-water 

partition coefficient (KOW) of the chemical and the normalized whole-body metabolic 

biotransformation rate constant as input parameters to predict BAF values. Model predictions 

may be highly uncertain for chemicals that have estimated log KOW values > 9. The model is not 

recommended at this time for chemicals that appreciably ionize, for pigments and dyes, or for 

perfluorinated substances (USEPA, 2012a). 

For bromoform, BAFs have been estimated using the EPI Suite model (USEPA, 2012a) as 

described above for trophic levels 2, 3, and 4. The estimated lower (TL2), mid (TL3), and upper 

(TL4) trophic level BAFs for bromoform (log KOW = 2.40) are 13.85 L/kg, 15.18 L/kg, and 19.49

L/kg wet-weight, respectively. These estimated BAFs replace the bioconcentration factor (BCF) 

for chloroform of 3.75 L/kg used in the 2002 criteria derivations, which was calculated from a 

measured steady-state bioconcentration of chloroform from a study of bluegills and 

represented all trophic levels (USEPA, 1980).

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Bromoform 75-25-2 

Hazard Identification and Dose Response: Cancer Slope Factor

Bromoform is characterized as “likely to be carcinogenic to humans” following the 1999 

EPA Guidelines for Carcinogen Risk Assessment (USEPA, 1999; USEPA; 2001; USEPA, 2005).

The cancer slope factor (CSF) is an upper bound, approximating a 95% confidence limit, 

on the increased cancer risk from a lifetime oral exposure to an agent. The previously used CSF 

for bromoform was 7.9 × 10–3 per mg/kg·day (USEPA, 2005). In 2005, EPA’s Office of Water 

updated the toxicology assessment for bromoform. That assessment (USEPA, 2005), which has 

been subject to extensive internal and external peer review, provides the basis for the updated 

CSF of 4.6×10–3 per mg/kg·day (0.0046 per mg/kg·day) used to update the AWQC.

The principle study by the National Toxicology Program (NTP, 1989) chosen to calculate 

the cancer slope factor for bromoform was based on development of tumors of the large 

intestine in female rats with oral exposure to bromoform (USEPA, 2005).

Criteria Derivation- Analysis

Table 1 summarizes model inputs used to derive the 2014 updated human health 

ambient water quality criteria for bromoform. Criteria calculations are presented below. These 

updated bromoform criteria recommendations are based on the 2000 Methodology and 

updated exposure assumptions as described above (Exposure Factors).

Table 1. Summary of input parameters for 2014 human health ambient water quality criteria 

for bromoform

Component Value Source Input Characterization

CSF 0.0046 per mg/kg·day USEPA, 

2005

an upper bound, approximating a 95% 

confidence limit, on the increased cancer risk 

from a lifetime exposure to an agent by 

ingestion

BW 80 kg USEPA, 

2011 mean weight for adults (≥21 years of age)

DI 3 L/day USEPA, 

2011 90th percentile for adults (≥21 years of age)

FCR

TL2 0.008 kg/day USEPA, 

2014

90th percentile consumption rate for the U.S. 

adult population (≥21 years of age) TL3 0.009 kg/day

TL4 0.005 kg/day

BAF

TL2 13.85 L/kg

USEPA, 

2012a

EPI Suite KOW model estimated steady-state 

BAF values for non-ionic organic chemicals in 

3 general trophic levels of fish in temperate 

environments (10°C)

TL3 15.18 L/kg

TL4 19.49 L/kg

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For consumption of water and organisms:

AWQC (μg/L) = [10-6 / CSF (kg·d/mg)] × BW (kg) × 1000 (μg/mg)

 DI (L/d) + ∑ (FCRi (kg/d) × BAFi (L/kg)) 4

i=2

 = 10-6 / 0.0046 kg·d/mg × 80 kg × 1000 μg/mg

 3 L/d + ((0.008 kg/d × 13.85 L/kg) + (0.009 kg/d × 15.18 L/kg) + (0.005 kg/d × 19.49 L/kg))

 = 5.199 μg/L (rounded to 5.2 μg/L because of significant figures, see 2000 Methodology)

For consumption of organisms only:

AWQC (μg/L) = [10-6 / CSF (kg·d/mg)] × BW (kg) × 1000 (μg/mg)

 ∑ (FCRi (kg/d) × BAFi (L/kg)) 4

i=2

 = 10-6 / 0.0046 kg·d/mg × 80 kg × 1000 μg/mg 

 (0.008 kg/d × 13.85 L/kg) + (0.009 kg/d × 15.18 L/kg) + (0.005 kg/d × 19.49 L/kg)

 = 50.428 μg/L (rounded to 50 μg/L because of significant figures, see 2000 Methodology)

The updated magnitude of the human health ambient water quality criteria for 

bromoform at the 10-6 risk level are 5.2 μg/L (water and organism) and 50 μg/L (organism only) 

(Table 2). These updated criteria replace the previously published values (USEPA, 2002b).

Table 2. Summary of EPA’s preciously recommended (2002) and updated (2014) human health 

ambient water quality criteria for bromoform

2002 Human Health AWQC 2014 Human Health AWQC

Water and Organism 4.3 μg/L 5.2 μg/L

Organism Only 140 μg/L 50 μg/L

These AWQC are meant to be protective of human health for the general adult 

population from an increased cancer risk due to exposure to bromoform at a 10-6 or 1 in 

1,000,000 risk level. The 10-6 risk level associated with the AWQC represents the concentration 

that would be expected to increase an individual’s lifetime cancer risk from exposure to the 

particular pollutant by no more than one chance in one million, regardless of the additional 

lifetime cancer risk due to exposure, if any, to that particular substance from other sources. 

 

Criteria Characterization

The updated 2014 human health AWQC for bromoform take into account current data 

on health effects and exposure input parameters, consistent with the 2000 Methodology. The 

updated 2014 human health AWQC for bromoform are about one-third higher or lower than 

EPA’s previously recommended 2002 criteria; i.e. the water-and-organism criterion increased

from 4.3 to 5.2 μg/L and the organism-only criterion decreased from 140 to 50 μg/L (Table 2). 

The following paragraphs describe the individual influence of each of the revised model inputs 

and exposure assumptions on the overall change in value. 

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Bromoform 75-25-2 

Body Weight

EPA’s updated AWQC assume a higher body weight compared to the previously

recommended 2002 criteria, reflecting a recent rise in average adult body weight among the 

U.S. population. The updated body weight assumption of 80 kg based on recent survey data 

from the 1999−2006 NHANES data is 10 kg greater than the previous recommendation of 70 kg.

Assuming all other input parameters remain constant, a higher average body weight in the 

AWQC calculations (Eq. 1 and 2 above) results in higher AWQC. That is, as body weight 

increases, the level of a contaminant in water at or below which negative health effects are not 

anticipated from a lifetime of exposure also increases. 

Drinking Water Intake

The updated drinking water intake assumption is 3 L/day, which is higher than the 

previously recommended rate of 2 L/day. Assuming all other input parameters remain constant, 

a higher drinking water intake assumption in the AWQC calculations (Eq. 1 and 2 above) results 

in lower AWQC. That is, as drinking water intake increases, and thus overall exposure increases, 

the level of a contaminant in water at or below which negative health effects are not 

anticipated from a lifetime of exposure decreases. 

Fish Consumption Rate

The updated fish consumption rate is 22 g/day, divided into trophic level rates of 8 

g/day, 9 g/day, and 5 g/day for trophic levels 2, 3, and 4, respectively, which is higher than the 

previously recommended rate of 17.5 g/day. Assuming all other input parameters remain 

constant, a higher fish consumption rate assumption in the AWQC calculations (Eq. 1 and 2 

above) results in lower AWQC. That is, as fish consumption increase, and thus overall exposure 

increases, the level of a contaminant in water at or below which negative health effects are not 

anticipated from a lifetime of exposure decreases.

Bioaccumulation Factor

The updated AWQC rely on EPI Suite model-estimated BAFs rather than the previously 

recommended BCF of 3.75 L/kg. The lower (TL2), mid (TL3), and upper (TL4) trophic level BAFs 

assumed in the updated criteria equations (Eq. 1 and 2 above) are 13.85, 15.18, and 19.49 L/kg 

wet-weight, respectively. Assuming all other input parameters remain constant, the higher

ratios of fish tissue concentrations to water concentrations of bromoform by aquatic organisms 

result in lower AWQC. That is, as bioaccumulation or bioconcentration of a contaminant in fish 

and shellfish increases, and thus overall exposure increases, the level of a contaminant in water 

at or below which negative health effects are not anticipated from a lifetime of exposure 

decreases. The utilization of a bioaccumulation factor rather than a bioconcentration factor 

better represents the amount of a contaminant accumulating in an organism because it 

accounts not only for the organism’s exposure to the pollutant in the water column, but also 

from the food chain and surrounding environment as well as biotransformation of the pollutant 

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Bromoform 75-25-2 

in the organism due to metabolic processes. The utilization of the three trophic levels of fish 

and shellfish consumed, as opposed to representing all trophic levels of fish and shellfish 

consumed by a single value, allows for better exposure representation, especially when 

pollutants bioaccumulate in significantly different amounts for organisms in different trophic 

levels at different positions in the food chain.

Cancer Slope Factor

The updated CSF of 4.6 × 10–3 per mg/kg·day replaces the higher value of 7.9 × 10–3 per 

mg/kg·day. The change in the CSF was due to a reevaluation of the key study utilizing the 

linearized multistage (LMS) method and an animal-to-human conversion factor of body 

weight3/4 rather than body weight2/3 as was done previously (USEPA, 2005). Assuming all other 

input parameters remain constant, a lower CSF in the AWQC calculations (Eq. 1 and 2 above) 

results in higher AWQC. That is, as the CSF decreases, the level of a contaminant in water at or 

below which the number of individuals at a specified risk level of developing cancer above the 

background rate increases. 

Additional routes of exposure to a particular pollutant besides exposure from water 

intake and fish consumption are possible and may be considered when setting criteria for a 

state or tribe. Possible additional routes include, but are not limited to, dermal exposure, 

inhalation exposure, marine fish and shellfish consumption (when not included in the fish 

consumption rate), and non-fish dietary exposures (fruits, vegetables, grains, meats or poultry). 

If scientific evidence exists which indicate that one or more of these routes pose a significant 

risk of exposure, states and authorized tribes are encouraged to ensure that subpopulations are 

adequately protected.

States, territories, and authorized tribes have the flexibility to develop criteria, on a sitespecific basis, that provide additional protection appropriate for highly exposed populations. 

EPA is aware that exposure patterns in general, and fish consumption in particular, vary 

substantially. EPA understands that highly exposed populations may be widely distributed 

geographically throughout a given state, territory, or authorized tribal area. EPA recommends 

that priority be given to identifying and adequately protecting the most highly exposed 

populations. Thus, if a state, territory, or authorized tribe determines that a highly exposed 

population is at greater risk and would not be adequately protected by criteria based on the 

general population, and by the national 304(a) criteria in particular, the state, territory, or 

authorized tribe may adopt more stringent criteria using alternative exposure assumptions 

(USEPA, 2000). Subpopulations that may be considered include, but are not limited to, 

recreational fishers, subsistence fishers, women of childbearing age, and children. When 

scientific data exist showing one of these subpopulations is at risk of greater exposure to the 

pollutant or are biologically more sensitive, then the relevant inputs should be considered in 

setting criteria. This could entail raising the fish consumption rate to a level more reflective of 

the subpopulation based on collected data or adjusting the body weight and drinking water 

intake.

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Bromoform 75-25-2 

Chemical Name / Synonyms

• Bromoform (CAS Number 75-25-2)

• Methane, tribromo- 

• Methenyl tribromide

• Tribromomethane

References

NTP (National Toxicology Program). 1989. Toxicology and carcinogenesis studies of 

tribromomethane (bromoform) in F344/N rats and B6C3F1 mice (gavage studies). 

Technical Report Series No. 350. Research Triangle Park, NC: U.S. Department of Health 

and Human Services.

USEPA (U.S. Environmental Protection Agency). 1980. Ambient Water Quality Criteria for 

Chloroform. Office of Water. Washington, DC. EPA-440-5-80-033.

http://water.epa.gov/scitech/swguidance/standards/upload/2001_10_12_criteria_ambi

entwqc_chloroform80.pdf.

USEPA (U.S. Environmental Protection Agency). 1999. Guidelines for Carcinogen Risk 

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http://www.epa.gov/raf/publications/pdfs/CANCER_GLS.PDF. 

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EPA-822-B-00-004.

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Additional Information and Comment. Federal Register 66: 59593-59594.

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in the United States. Washington, DC. EPA-821-C-02-003.

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on_report.pdf. 

USEPA (U.S. Environmental Protection Agency). 2002b. National Recommended Water Quality 

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http://water.epa.gov/scitech/swguidance/standards/upload/2008_04_29_criteria_wqct

able_nrwqc-2002.pdf.

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Bromoform 75-25-2

USEPA (U.S. Environmental Protection Agency). 2005. Drinking Water Criteria Document For 

Brominated Trihalomethanes. Office of Water. Washington, DC. EPA-822-R-05-011.

http://water.epa.gov/action/advisories/drinking/upload/2006_05_04_criteria_drinking_

brthm-200605-508.pdf.

USEPA (U.S. Environmental Protection Agency). 2011. Exposure Factors Handbook: 2011 

Edition. Office of Research and Development. Washington, DC. EPA-600-R-09-052F.

http://www.epa.gov/ncea/efh/pdfs/efh-complete.pdf.

USEPA (U.S. Environmental Protection Agency). 2012a. Estimation Programs Interface SuiteTM 

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Washington, DC, USA. http://www.epa.gov/oppt/exposure/pubs/episuite.htm.

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EPA-601-D-12-001. http://www.epa.gov/raf/files/framework-document-7-13-12.pdf.

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the U.S. Population and Selected Subpopulations (NHANES 2003-2010). Office of Water. 

Washington, DC. EPA-820-R-14-002.

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