**Certification      of the Principal Executive Officer and Chief Financial      Officer**

**Under      Section 302 of the Sarbanes-Oxley Act**

| 1. | I                  have reviewed Amendment No. 2 to this Annual Report on Form 10-KSB                  for the                  fiscal year ended December 31, 2006 of Lixte Biotechnology Holdings,                  Inc.; |
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| 2. | Based                on my knowledge, this report does not contain any untrue statement                of a                material fact or omit to state a material fact necessary to make                the                statements made, in light of the circumstances under which such statements                were made, not misleading with respect to the period covered by this                report; |
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| 3. | Based                on my knowledge, the financial statements, and other financial information                included in this report, fairly present in all material respects                the                financial condition, results of operations and cash flows of the                issuer as                of, and for, the periods presented in this                report; |
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| 4. | I                am responsible for establishing and maintaining disclosure controls                and                procedures (as defined in Exchange Act Rules 13a-15(e) and 15d-15(e))                for                the issuer and have: |
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|   | (a) | Designed                such disclosure controls and procedures, or caused such disclosure                controls and procedures to be designed under our supervision, to                ensure                that material information relating to the issuer, including its                consolidated subsidiaries, is made known to us by others within those                entities, particularly during the period in which this report is                being                prepared; |
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|   | (b) | Evaluated                the effectiveness of the issuer’s disclosure controls and procedures and                presented in this report our conclusions about the effectiveness                of the                disclosure controls and procedures, as of the end of the period covered                by                this report based on such evaluation;                and |
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|   | (c) | Disclosed                in this report any change in the issuer’s internal control over financial                reporting that occurred during the issuer’s most recent fiscal quarter                that has materially affected, or is reasonably likely to materially                affect, the issuer’s internal control over financial reporting;                and |
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| 5. | I                have disclosed, based on my most recent evaluation of internal control                over financial reporting, to the issuer’s auditors and the audit committee                of issuer’s board of directors (or persons performing the equivalent                functions): |
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|   | (a) | All                significant deficiencies and material weaknesses in the design or                operation of internal control over financial reporting which are                reasonably likely to adversely affect the issuer’s ability to record,                process, summarize and report financial information;                and |
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|   | (b) | Any                fraud, whether or not material, that involves management or other                employees who have a significant role in the issuer’s internal control                over financial reporting. |
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| Date:                  May 14, 2007 | By:                                                                                              | /s/ John                S.                Kovach |
|                                | Name:                John Kovach                                                                           |
|                                | Title:                  Chief Executive Officer and  Chief                Financial Officer |

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