# Patent

## Title
Genes encoding a novel type of lysophophatidylcholine acyltransferases and their use to increase triacylglycerol production and/or modify fatty acid composition

## Abstract

Described nucleic acid molecules (and corresponding peptides) encode lyso-phosphatidylcholine (LPC) acyltransferases. Over-expression of the LPC acyltransferases in a cell may lead to enhanced production of PUFA, or other unusual fatty acids, and/or to increased oil content in the cell.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This patent application is a divisional of U.S. patent application Ser. No. 12/448,061, filed Oct. 1, 2009, now U.S. Pat. No. 8,383,886, which application is a national phase entry under 35 U.S.C. §371 of International Patent Application PCT/US2007/025650, filed Dec. 13, 2007, designating the United States of America and published in English as International Patent Publication WO 2008/076377 on Jun. 26, 2008, which claims priority, under the Paris Convention, to U.S. patent application Ser. No. 11/820,014, filed on Jun. 15, 2007, now U.S. Pat. No. 7,732,155, issued Jun. 19, 2008, which claims the benefit under Article 8 of the Patent Cooperation Treaty and under 35 U.S.C. §119(e) to U.S. Provisional Patent Application Ser. No. 60/874,497, filed on Dec. 13, 2006, the contents of the entirety of each of which are incorporated herein by this reference.


STATEMENT ACCORDING TO 37 C.F.R. §1.821(C) OR (E)—SEQUENCE LISTING SUBMITTED AS A TXT AND PDF FILES
Pursuant to 37 C.F.R. §1.821(c) or (e), files containing a TXT version and a PDF version of the Sequence Listing has been submitted concomitant with this application, the contents of which are hereby incorporated by reference.
TECHNICAL FIELD
The disclosure relates generally to biotechnology, and, more particularly, to lyso-phosphatidylcholine (LPC) acyltransferase, polynucleotides that encode LPC acyltransferases, and associated means and methods.
BACKGROUND
Phosphatidylcholine (PC) serves not only as a major component of cellular membranes, but also as a major source of fatty acyl donors for triacylglycerol biosynthesis in eukaryotic organisms. At least three pathways through which PC is generated exist: (i) the CDP-choline pathway where diacylglycerol (DAG) is a direct precursor; (ii) a pathway where CDP-DAG is a direct precursor, involving phosphatidylserine formation and decarboxylation and phosphatidylethanolamine methylation (Zheng and Zou, 2001); and (iii) a pathway with LPC as substrate. The third pathway is exerted by LPC acyltransferases (LPCAT).
LPCAT enzymes catalyze the acylation of LPC molecules to form PC and play a pivotal role in membrane biogenesis. They can also exert a reversible reaction to release the fatty acyl chain esterified to the sn-2 position of PC, thereby contributing to a continuous remodeling of fatty acyl-CoA and PC pools.
The significance of LPCAT in glycerolipid metabolism of eukaryotic systems has been noted for many years. For genetic engineering of plant-based production of very long chain polyunsaturated fatty acid (PUFA), this enzyme is believed to represent a bottleneck for acyl exchange between the fatty acyl elongation and de-saturation systems. In higher plants, the function of this enzyme is largely unknown, but it has been proposed that the enzyme is involved in the selective incorporation of fatty acids into a storage pool.
Although LPCAT relating to the synthesis of surfactant lipid located on the surface of (pulmonary) cells have been reported in mammalian systems (X. Chen et al., PNAS 2006 103:11724-11729; H. Nakanishi et al., JBC 2006 281:20140-20147), an LPC acyltransferase involved in membrane or storage lipid synthesis has not been reported.
Recently, a mitochondrial acyl-CoA independent LPCAT from Saccharomyces cerevisiae has been identified. This enzyme has been shown to function in cardiolipin metabolism (Testet et al. 2005). In addition, Shindou et al. (2007) reported that aceyl-CoA:lyso-PAF (platelet-activating factor) acetyltransferase possesses LPCAT activity.
DISCLOSURE
Novel types of LPCAT enzymes whose sequences are unrelated to any known LPCAT enzymes have been identified. Known domains for other sn-2 acyltransferases such as the mammalian LPC acyltransferases are not identifiable in the LPC acyltransferase assay disclosed herein.
Previously reported LPCAT enzymes share a substantial sequence homology to glycerol-3-phosphate acyltransferase and lysophosphatidic acyltransferase. In contrast, the LPCAT sequences disclosed herein are unrelated to any known LPCAT sequences, and belong to a new class of LPCAT. Four conserved motifs were identified in this novel class of LPCAT enzymes. The identified motifs are different from previously reported LPCAT, which contain motifs having a high degree of similarity to those in other known acyltransferases employing glycerol-3-phosphate and lysophosphatidic acid as substrates. In contrast, sequence information of the motifs identified herein is novel, and can lead to the identification of new class of LPCAT genes from a broad spectrum of species.
Thus, in certain embodiments, a lyso-phosphatidylcholine acyltransferase gene or class of genes is identified. The LPC acyltransferase gene may be expressed or overexpressed in a cell and used to modify glycerolipid biosynthesis in a cell. Such an LPC acyltransferase gene may be expressed or overexpressed in a cell and used to modulate or enhance production of fatty acids, especially polyunsaturated fatty acids (PUFA) or other unusual fatty acids, and/or to increased oil content in the cell. The LPC acyltransferase gene may be expressed or overexpressed in planta in order to modify glycerolipid biosynthesis in a plant. In certain embodiments, the LPC acyltransferase gene is expressed or overexpressed, in planta, in order to enhance the production of fatty acids in a plant.
In certain embodiments, a vector is provided having an LPC acyltransferase gene of the invention. The vector may be used to transform a cell, thus producing a recombinant cell having the LPC acyltransferase gene. The cell may comprise, for example, a bacterial cell, a yeast cell, or a plant cell. In certain embodiments, a plant, plant seed or progeny thereof includes a cell having a recombinant LPC acyltransferase gene.
In other embodiments, knock-out mutants disrupted in LPC acyltransferase gene of yeast and plants are identified.
In certain embodiments, a recombinant cell expresses an LPC acyltransferase gene and produces an LPC acyltransferase polypeptide that may be isolated or purified from the cell.
In certain embodiments, nucleotide and deduced amino acid sequences associated with an LPC acyltransferase gene are disclosed. The sequence, or a portion thereof, may be used to identify genes from other species that encode polypeptides with LPC acyltransferase activity.
In certain embodiments, a process for producing fatty acids includes transforming a cell with an LPC acyltransferase gene. The transformed cell expresses the fatty acid acyltransferase gene and produces fatty acids. The fatty acids may be isolated or purified from the recombinant cell or culture media in which the cell grows, and subsequently incorporated into a composition.
In certain embodiments, a peptide comprising one or more of the four motifs identified herein may be used as an LPC Acyltransferase. Similarly, a nucleotide sequence encoding a peptide comprising one or more of the four motifs may be used as an LPC Acyltransferase.
Provided is an isolated or recombinant nucleic acid molecule encoding an LPC acyltransferase, and a cell transformed with the isolated or recombinant nucleic acid molecule as described herein. Also provided is a process for increasing fatty acid production in a cell, the process comprising: transforming a cell with a nucleic acid molecule encoding an LPC acyltransferase; and, growing the cell under conditions wherein the LPC acyltransferase is expressed. Also provided is a use of an isolated or recombinant nucleic acid molecule encoding an LPC acyltransferase for producing an LPC acyltransferase in a cell. Also provided is a purified or an isolated LPC acyltransferase.
LPCAT enzymes play a critical role in remodeling fatty acid and PC pools as well as PC synthesis. The remodeled fatty acyl chains in the form of acyl-CoA or esterified at the sn-2 position of PC can be used for triacylglycerol synthesis. Thus, this novel type of LPCAT isolated from the organisms where very-long-chain polyunsaturated fatty acids (VLCPUFA) are present at a high level can be used to increase the production of VLCPUFA. As well, this novel type of LPCAT isolated from species containing high amount of unusual fatty acids can be used to increase the production of unusual fatty acids. For instance, LPCAT enzymes isolated from castor bean are useful in increasing the production of hydroxyl fatty acids in oil seeds.
The enzyme activity described herein provides support that the motif-based gene searching is a useful approach.



BRIEF DESCRIPTION OF THE DRAWINGS
The patent application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawings will be provided by the Office upon request and payment of the necessary fee.
FIG. 1 is a graph of LPCAT activity (nmol/mg·h) of wild-type (WT) and YOC175c mutant yeast strains.
FIG. 2 is an alignment of LPCAT sequences from different species that revealing, among other things, four conserved motifs unique for this type of LPCAT enzymes.
FIG. 3 is another alignment of LPCAT sequences from different plant species that revealed four conserved motifs (SEQ ID NOS:81-84).
FIG. 4 depicts that the expression of the TpLCAT in an 1 pcat mutant was able to complement the sensitivity of the 1 pcat mutant to Lyso-PAF.
FIG. 5 is a graph showing the expression of TpLPCAT in yeast. LPCAT assays were performed on cell lysates of yeast 1 pcat mutant strain By02431 transformed with TpLPCAT/pYES2.1 and pYes2.1/V5-His-TOPO plasmid only (control) in the presence of 14C-Lyso-PC and different acyl-COAs.
FIG. 6 comprises TLC plates of LPCAT assays on cell lysates of yeast lpcat mutant strain By02431 transformed with TpLPCAT/pYES2.1 and pYes2.1/V5-His-TOPO plasmid only (control) in the presence of 14C-Lyso-PC and different acyl-CoAs. 1, 3, 5, 7, 9, 11, and 13-TpLPCAT; 2, 4, 6, 8, 10, 12, and 14—empty vector.
FIG. 7 shows a LysoPAF sensitivity test of YOR175c mutant, AtLPCATs transformant, wherein A is VO/BY02431, B is AtLPCAT1/BY02431, and C is AtLPCAT2/BY02431.
FIG. 8 is a graph showing the Lyso-lipid substrate specificity of Arabidopsis LPCATs.
FIG. 9 is a graph comparing LPAAT and LPCAT activity of slc1Δ, lca1Δ, and congenic WT yeast strain. Cell lysates equivalent to 200 μg protein were assayed for acylation of oleoyl-LPA and oleoyl-LPC with [14C] oleoyl-CoA. The reaction mixture contained 45 μM 18:1-LPA or 18:1-LPC, 18 μM (10 nCi/nmol) 18:1-CoA. The results are presented as a mean of three assays.
FIG. 10 is graph depicting lysophospholipid acyltransferase activity in lca1Δ and its congenic WT yeast strains. Microsomal preparations were assayed for acylation of palmitoyl-LPA, LPC, LPE, LPG, LPI, and LPS with [14C] palmitoyl-CoA. The reaction mixture contained 45 μM lysophospholipid, 27 μM (10 nCi/nmol) 16:1-CoA and 50 μg protein. The results are presented as a mean of three assays.
FIG. 11 is a graph showing the substrate specificity of LCA1. The assays were performed with 3 μg microsomal protein from lca1Δ harboring an empty vector (V0) and lca1Δ expressing LCA1. The reaction contained 112.5 μm [14C] palmitoyl-CoA (5.5 nCi/nmol) and 50 μM lysophospholipid substrate (LPA, LPC, LPE, LPG, LPI, and LPS). Reaction was allowed for two minutes with 100 rpm shaking. The results are presented as a mean of three assays.
FIG. 12 shows the lyso-PAF and lyso-PC Sensitivity test lca1Δ, WT and lca1delta over-expressing LCA1. Cells were frown first in SC-URA+2% glucose media overnight then in protein expression induction media for six hours. Cultures were diluted to OD600 value of OD600 0.5, 1, 2, 3, respectively, from which 5 μl were inoculated (from left to right) onto YPD plate containing lyso-PAF or lyso-PC. The plates were incubated at 28° C. for 36 hours.
FIG. 13 depicts two graphs showing substrate preference of LCA1. A. Acyl-CoA substrate preference. Assays were performed with 3 μg microsomal protein from lca1Δ harboring an empty vector (V0) and lca1Δ expressing LCA1, with 112.5 μM [14C] palmitoyl-CoA (1.35 nCi/nmol) and 50 μM acyl-CoA species. B. LPC substrate preference. Assays were performed with 3 μg microsomal protein, 112.5 μM [14C] palmitoyl-CoA (5.5 nCi/nmol) and 50 μM lysophospholipid species. The values for lca1Δ are not shown. The results are presented as a mean of three assays.
FIG. 14 depicts a TLC pattern of choline-containing compounds in the TCA fraction for PC turnover assessment. Samples were chromatographed and visualized on MERCK® Silica Gel G60 plate as described herein. Lane 1, TCA fraction of WT yeast; lane 2, TCA fraction of lca1Δ yeast; lane 3, [14C] choline; lane 4, palmitoleoyl-LPC; lane 5, GroPC; lane 6, phosphocholine; and lane 7, CDP-choline. Dashed line indicated sample origin.
FIG. 15 is a graph illustrating lyso-lipid substrate specificity of AtLPCATs expressed in lca1Δ. The assays were preformed with 3 μg microsomal protein from lca1Δ harboring an empty vector (VO) and lca1Δ expressing AtLPCAT1 and AtLPCAT2. The reaction contained 45 μM [14C] palmitoyl-CoA (5.5 nCi/nmol) and 45 μM Lysophospholipid substrate (LPA, LPC, LPE, LPG, LPI and LPS). The reaction was allowed for ten minutes with 100 rpm shaking. The results were presented as a mean of three assays.
FIG. 16 depicts plates showing LysoPAF Sensitivity test of a yor175c mutant strain transformed with empty pYES2.1, pYES2.1::AtLPCAT1 and pYES2.1::LPCAT2. Cells were grown first in SC-URA+2% glucose media overnight then in protein expression induction media for six hours. Cultures were diluted to OD600 value of OD600=1, 2, 4, respectively, from which 5 μl was inoculated (from left to right) onto YPD plate containing Lyso-PAF or Lyso-PC. The plates were incubated at 28° C. for 36 hours.
FIG. 17 is a graph depicting the Acyl-CoA preference assessment of AtLPCATs expressed in lca1Δ. Assays were preformed with 20 μg microsomal protein from lca1Δ harboring an empty vector (VO) and lca1Δ expressing AtLPCAT1 and AtLPCAT2, with 45 μM [14C]palmitoyl-LPC (1.35 nCi/nmol) and 45 μM acyl-CoA species.
FIG. 18 is a graph depicting the LPC substrate preference of AtLPCATs expressed in lca1Δ. Assays were preformed with 20 μg microsomal protein, 45 μM [14C]palmitoyl-CoA (5.5 nCi/nmol), 45 μM LPC species. The results were presented as a mean of three assays.
FIG. 19 is a graph depicting the inhibitory effect of Zn2+ on AtLPCATs activity. The lca1Δ over-expressing AtLPCATs was used to asses Zn2+ effect. The reactions contained 25 μM palmitoyl-LPC (1.35 nCi/nmol), 20 μg microsomal proteins, 0.1 M HEPES (pH 7.4), 25 μM stearyl-CoA and indicated concentration of ZnCl2.
FIG. 20 is a sequence alignment of YOR175c with its selected human and mouse homologs. Alignment was performed with CLUSTALV from the DNASTAR package run with default multiple alignment parameters (gap opening penalty: 10; gap extension penalty: 10).



DETAILED DESCRIPTION
Preferably, the nucleic acid molecule encoding the LPC acyltransferase is derived from yeast, plant, or mammalian species. Yeast species include, for example, species of the genus Saccharomyces, for example, Saccharomyces cerevisiae. Plant species include, for example, species of the family Brassicaceae. Of the family Brassicaceae, species of genus Brassica and genus Arabidopsis are of particular note, for example, Arabidopsis thaliana. Mammalian species include mouse and human.
In particular, provided are a nucleic acid molecule encoding an LPC acyltransferase from S. cerevisiae and two nucleic acid molecules encoding two different isoforms of LPC acyltransferase from A. thaliana. There is also provided the LPC acyltransferases encoded by the herein described nucleic acid molecules.
Provided herein is an isolated or recombinant nucleic acid molecule having a nucleotide sequence encoding an LPC acyltransferase such as amino acid sequence comprising SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:11; SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17; SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39; SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:85, SEQ ID NO:86, SEQ ID NO:87, or SEQ ID NO:88. In particular, there is provided an isolated or recombinant nucleic acid molecule having a nucleotide sequence comprising SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, or SEQ ID NO:34. Preferably, the LPC acyltransferase encoded by a nucleic acid molecule comprises an amino acid sequence comprises an amino acid sequence having at least 60% homology to the aforementioned sequences. Homology is more preferably at least 70%, 80%, 90%, or 95%. It will be appreciated that this disclosure embraces the degeneracy of codon usage as would be understood by one of ordinary skill in the art.
Homologs of the LPC acyltransferase genes described herein obtained from other organisms, for example, plants, may be obtained by screening appropriate libraries that include the homologs, wherein the screening is performed with the nucleotide sequence of the specific LPC acyltransferase genes of the instant invention or portions or probes thereof, or identified by sequence homology search using sequence alignment search programs such as BLAST, FASTA.
Further included are nucleic acid molecules that hybridize to the above disclosed sequences. Hybridization conditions may be stringent in that hybridization will occur if there is at least a 90%, 95% or 97% identity with the nucleic acid molecule that encodes the LPC acyltransferase of the instant invention. The stringent conditions may include those used for known Southern hybridizations such as, for example, incubation overnight at 42° C. in a solution having 50% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5×Denhardt's solution, 10% dextran sulfate, and 20 micrograms/milliliter denatured, sheared salmon sperm DNA, following by washing the hybridization support in 0.1×SSC at about 65° C. Other known hybridization conditions are well known and are described in Sambrook et al., Molecular Cloning: A Laboratory Manual, Third Edition, Cold Spring Harbor, N.Y. (2001), incorporated herein in its entirety by this reference.
Nucleic acid molecules that code for an LPC acyltransferase may be transformed into an organism, for example, a plant. As known in the art, there are a number of ways by which genes and gene constructs can be introduced into organisms, for example, plants, and a combination of transformation and tissue culture techniques have been successfully integrated into effective strategies for creating transgenic organisms, for example, crop plants. These methods, which can be used in the invention, have been described elsewhere (Potrykus, 1991; Vasil, 1994; Walden and Wingender, 1995; Songstad et al., 1995), and are well known to persons skilled in the art. For example, one skilled in the art will certainly be aware that, in addition to Agrobacterium-mediated transformation of Arabidopsis by vacuum infiltration (Bechtold et al., 1993) or wound inoculation (Katavic et al., 1994), it is equally possible to transform other plant and crop species, using Agrobacterium Ti-plasmid-mediated transformation (e.g., hypocotyl (DeBlock et al., 1989) or cotyledonary petiole (Moloney et al., 1989) wound infection), particle bombardment/biolistic methods (Sanford et al., 1987; Nehra et al., 1994; Becker et al., 1994) or polyethylene glycol-assisted, protoplast transformation (Rhodes et al., 1988; Shimamoto et al., 1989) methods.
As will also be apparent to persons skilled in the art, and as described elsewhere (Meyer, 1995; Dada et al., 1997), it is possible to utilize plant promoters to direct any intended up- or down-regulation of transgene expression using constitutive promoters (e.g., those based on CaMV35S), or by using promoters which can target gene expression to particular cells, tissues (e.g., napin promoter for expression of transgenes in developing seed cotyledons), organs (e.g., roots), to a particular developmental stage, or in response to a particular external stimulus (e.g., heat shock).
Promoters for use herein may be inducible, constitutive, or tissue-specific or have various combinations of such characteristics. Useful promoters include, but are not limited to constitutive promoters such as carnation etched ring virus (CERV), cauliflower mosaic virus (CaMV) 35S promoter, or more particularly the double enhanced cauliflower mosaic virus promoter, comprising two CaMV 35S promoters in tandem (referred to as a “Double 35S” promoter).
It may be desirable to use a tissue-specific or developmentally regulated promoter instead of a constitutive promoter in certain circumstances. A tissue-specific promoter allows for overexpression in certain tissues without affecting expression in other tissues. By way of illustration, a preferred promoter used in overexpression of enzymes in seed tissue is an ACP promoter as described in PCT International Publication WO 92/18634, published Oct. 29, 1992, the disclosure of which is herein incorporated by reference.
The promoter and termination regulatory regions will be functional in the host plant cell and may be heterologous (that is, not naturally occurring) or homologous (derived from the plant host species) to the plant cell and the gene. Suitable promoters which may be used are described above.
The termination regulatory region may be derived from the 3′ region of the gene from which the promoter was obtained or from another gene. Suitable termination regions which may be used are well known in the art and include Agrobacterium tumefaciens nopaline synthase terminator (Tnos), A. tumefaciens mannopine synthase terminator (Tmas) and the CaMV 35S terminator (T35S). Particularly preferred termination regions for use herein include the pea ribulose bisphosphate carboxylase small subunit termination region (TrbcS) or the Tnos termination region. Such gene constructs may suitably be screened for activity by transformation into a host plant via Agrobacterium and screening for increased isoprenoid levels.
Suitably, the nucleotide sequences for the genes may be extracted from, for instance, the GenBank® (a registered trademark of the U.S. Department of Health and Human Services) nucleotide database and searched for restriction enzymes that do not cut. These restriction sites may be added to the genes by conventional methods such as incorporating these sites in PCR primers or by sub-cloning.
Preferably, a DNA construct for use herein is comprised within a vector, most suitably an expression vector adapted for expression in an appropriate host (plant) cell. It will be appreciated that any vector which is capable of producing a plant comprising the introduced DNA sequence will be sufficient.
Suitable vectors are well known to those skilled in the art and are described in general technical references such as Pouwels et al., Cloning Vectors, A Laboratory Manual, Elsevier, Amsterdam (1986). Particularly suitable vectors include the Ti plasmid vectors.
Transformation techniques for introducing the DNA constructs into host cells are well known in the art and include such methods as micro-injection, using polyethylene glycol, electroporation, or high velocity ballistic penetration. A preferred method relies on Agrobacterium-mediated transformation. After transformation of the plant cells or plant, those plant cells or plants into which the desired DNA has been incorporated may be selected by such methods as antibiotic resistance, herbicide resistance, tolerance to amino-acid analogues or using phenotypic markers.
Various assays may be used to determine whether the plant cell shows an increase in gene expression, for example, Northern blotting or quantitative reverse transcriptase PCR (RT-PCR). Whole transgenic plants may be regenerated from the transformed cell by conventional methods. Such transgenic plants having improved isoprenoid levels may be propagated and self-pollinated to produce homozygous lines. Such plants produce seeds containing the genes for the introduced trait and can be grown to produce plants that will produce the selected phenotype.
Plants that may be modified or used for fatty acid production according to the instant invention include, without limitation, borage (Borago spp.), Canola, castor (Ricinus communis); cocoa bean (Theobroma cacao), corn (Zea mays), cotton (Gossypium spp), Crambe spp., Cuphea spp., flax (Linum spp.), Lesquerella and Limnanthes spp., Linola, nasturtium (Tropaeolum spp.), Oeanothera spp., olive (Olea spp.), palm (Elaeis spp.), peanut (Arachis spp.), rapeseed, safflower (Carthamus spp.), soybean (Glycine and Soja spp.), sunflower (Helianthus spp.), tobacco (Nicotiana spp.), Vernonia spp, wheat (Triticum spp.), barley (Hordeum spp.), rice (Oryza spp.), oat (Avena spp.) sorghum (Sorghum spp.), rye (Secale spp.) or other members of the Gramineae. It will further be apparent to those of ordinary skill in the art that genomic or sequence libraries of each of these plants may be screened with the nucleotide or amino acid sequences described herein (e.g., for one or more of the hereinafter identified conserved motifs (SEQ ID NO:46 through SEQ ID NO:49) for other sequences that encode or are homologous to sequences associated with the LPC acyltransferase of the instant invention.
Plants transformed with a nucleotide sequence of the instant invention that codes for an LPC acyltransferase may be grown. Seeds of the transgenic plants are harvested and fatty acids of the seeds are extracted. The extracted fatty acids are used for subsequent incorporation into a composition, for example, a pharmaceutical composition, a nutraceutical composition or a food composition.
In certain embodiments, a peptide comprising one or more of the four motifs may be used as an LPC Acyltransferase. Similarly, a nucleotide sequence encoding a peptide comprising one or more of the four motifs may be used as an LPC Acyltransferase.
Further described herein is a lyso-PAF sensitivity screen to identify novel LPCAT. This is detailed in FIGS. 12 and 16. For example, a method of screening for an LPCAT, wherein the method comprises expressing a candidate gene in a yeast LPCAT mutant, plating the yeast on to lyso-PAF plates, and detecting yeast colonies showing higher tolerance to the lyso-PAF, wherein the colonies showing higher tolerance indicate that the candidate gene is a LPCAT gene hereof. The candidate gene may be identified by screening a gene to determine the presence of one of more of nucleic acid sequences encoding at least one motif selected from the group consisting of SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:81, SEQ ID NO:82, SEQ ID NO:83, SEQ ID NO:84, and any combination thereof.
In certain embodiments, other methods of enhancing or altering oil production may also be used with the plant to be transformed (e.g., incorporating, for expression in the plant, a nucleic acid sequence selected from the group consisting of a nucleic acid sequence encoding a peptide having, for example, Brassica pyruvate dehydrogenase kinase activity (see, e.g., U.S. Pat. No. 7,214,859 to Marilla et al. (May 8, 2007), U.S. Pat. No. 6,500,670 to Zou et al. (December 2002), and U.S. Pat. No. 6,265,636 to Randall et al. (July 2001), the contents of the entirety of each of which is incorporated herein by this reference), a nucleic acid sequence encoding a peptide having diacylglycerol acyltransferase activity (see, e.g., U.S. Pat. No. 7,015,373 and U.S. Pat. No. 6,500,670 to Zou et al. (December 2002), the contents of the entirety of each of which is incorporated herein by this reference), a nucleic acid sequence encoding a peptide having glycerol-3-phosphate dehydrogenase activity (see, e.g., U.S. Pat. No. 7,112,724, the contents of the entirety of which is incorporated herein by this reference), and combinations thereof).
Also described is a method of transforming a cell or a plant, the method comprising introducing the isolated, purified or recombinant nucleic acid into the cell or plant. A process for producing a genetically transformed plant seed comprises introducing the nucleic acid into the plant seed.
Also described is a vector comprising SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, and/or SEQ ID NO:34.
Also described is a vector comprising a nucleic acid sequence encoding a polypeptide having lyso-phosphatidylcholine acyltransferase activity, wherein the nucleic acid sequence comprises SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, or SEQ ID NO:34, or a fragment of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, or SEQ ID NO:34, or having 90% identity with SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, or SEQ ID NO:34, wherein the fragment encodes the polypeptide having the lyso-phosphatidylcholine acyltransferase activity.
Also described is a method for increasing fatty acid production in a cell, the method comprising transforming a cell with a nucleic acid molecule encoding a lyso-phosphatidylcholine acyltransferase; and growing the cell under conditions wherein the lyso-phosphatidylcholine acyltransferase is expressed. The method can further comprise isolating the fatty acid. In such a method, the lyso-phosphatidylcholine acyltransferase preferably comprises at least one motif selected from the group consisting of SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, and any combination thereof.
Also described is a method of altering oil content in a plant comprising screening for a peptide encoded by a nucleotide sequence for at least one motif selected from the group consisting of SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, and SEQ ID NO:49; selecting the peptide based upon the presence of at least one of the four motifs; and expressing the nucleotide sequence encoding the peptide in the plant to alter the oil content of the plant.
Also described is a method of changing the oil content of a plant or plant seed, the method comprising introducing a nucleic acid construct comprising a nucleic acid sequence encoding a polypeptide selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:11; SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17; SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, and an amino acid sequence having at least 60% homology to any thereof having lyso-phosphatidylcholine acyltransferase activity into a plant transformation vector; transforming a genome of a plant or plant seed with the plant transformation vector; expressing the nucleic acid sequence; growing the plant or plant seed; and extracting the oil from the plant seed.
The methods can further comprise incorporating, for expression in the plant, a nucleic acid sequence selected from the group consisting of a nucleic acid sequence encoding a peptide having pyruvate dehydrogenase kinase activity, a nucleic acid sequence encoding a peptide having diacylglycerol acyltransferase activity, a nucleic acid sequence encoding a peptide having glycerol-3-phosphate dehydrogenase activity, and any combination thereof.
The invention is further described with the aid of the following illustrative Examples.
EXAMPLES
Example 1
Identification of Yeast LPC Acyltransferase Gene
Nucleotide sequences of nucleic acid molecules of the invention were identified through yeast genetic and functional screening. Yeast (S. cerevisiae) LPC acyltransferase gene was identified based on enzyme assays of yeast mutant strains in which the gene, YOR175c, was knocked out. The enzyme activity was assessed using 14C-labeled acyl-CoA and lyso-phosphatidylcholine. The reaction product of the lyso-phosphatidylcholine and radio-labeled acyl-CoA was separated through TLC and measured through scintillation counting. Deletion of the YOR175c gene in yeast resulted in a 90% reduction of LPC acyltransferase activity (FIG. 1). Therefore, YOR175c encodes LPC acyltransferase. Details are given below for the In Vitro Assay protocol for LPCAT (lyso-phosphatidylcholine (LPC) acyltransferase) activity.
We designate YOR175c as LCA1 in following description.
Gene expression vector construction: For TOPO TA-cloning and yeast complementation, Saccharomyces cerevisiae YOR175c ORF was PCR-amplified with primers FP: 5′ GGTGATTCTAGACTGCTGCTGATCGCTT 3′ (SEQ ID NO:91) and RP: 5′ GCATCTGTCGTTTTTGGAGCTCTACTCTT 3′ (SEQ ID NO:92), and cloned into pYES2.1 vector (Invitrogen). Correctly oriented plasmids were identified by DNA sequencing and subsequently introduced into YOR175c mutant yeast strain Y02431.
Microsomal preparation: Yeast strains were first grown in 15 ml of SC-Leu-His-Ura medium containing 2% glucose. Protein expression induction was carried out as described in Invitrogen manufacturer manual for yeast expression vector pYES2.1. After 24 hours of growth in SC+2% galactose+1% raffinose induction conditions, the cells were washed, first with distilled water and then with wall-breaking buffer (50 mM Tris-HCl, 1 mM EDTA, 0.6 M sorbitol, pH 7.4, 1 mM DTT). After centrifugation at 4,000 rpm (Eppendorf Centrifuge 5145C), the cells were resuspended in 1 ml wall-breaking buffer with 10 μl yeast protease cocktail (Sigma), and shaken vigorously in the presence of acid-washed glass beads (diameter 0.5 mm). The resultant homogenate was centrifuged at 12,000 rpm for ten minutes at 4° C. The decanted supernatant was further centrifuged at 100,000×g for 90-120 minutes at 4° C. The supernatant was discarded, and the pellet was suspended in homogenization buffer containing 20% glycerol and frozen at −80° C. until use. Protein concentration was measured using Bio-Rad Protein Assay Kit for final enzyme activity calculation.
In Vitro Assay of LPCAT activity: LPCAT substrate specificity was determined by measuring incorporation of [14C] lysophosphatidylcholine or [14C] palmitoyl-CoA into phosphatidylcholine. All assays were performed at least twice. For lysophospholipid substrate specificity assessment, 400 μl HEPES buffer contained 3 μg microsomal protein, 50 μM of lysophospholipid substrates and 112.5 μM [14C] palmitoyl-CoA (5.5 nCi/nmol). For acyl-CoA substrate selectivity analysis, 400 μl HEPES reaction buffer (pH7.4, 0.1 M) contained 3 μg microsomal protein, 50 μM acyl-CoA and 112.5 μM [14C] palmitoyl-PC (1.35 nCi/nmol). Reaction was allowed for 2 minutes at 30° C. with 100 rpm shaking. The reaction products were extracted with chloroform/methanol (2/1, v/v) and separated with Merck silica G60 TLC plates. Spots corresponding to different phospholipid species products were scraped off and 14C incorporation were scintillation counted. Different concentrations of ZnCl2 were added in to reactions for Zn2+ inhibitory effect assay.







TABLE 1







Inhibitory effect of Zn2+ on LCA1 activity










ZnCl2 concentration
LPCAT activity (% control)







0 mM (control)
100 ± 7.9 









20
mM
6 ± 2.0


0.1
mM
35 ± 22.4


25
μM
149.7 ± 12.0  


10
μM
136.8 ± 3.9   


5
μM
98 ± 5.9 









Results are expressed as means±S.D. The lca1Δ over-expressing LCA1 was used to assess Zn2+ effect. The reactions contained 5.6 μM palmitoyl-LPC (1.35 nCi/nmol), 1.5 μg microsomal proteins, 0.1 M HEPES (pH 7.4), 11.25 μM stearyl-CoA and indicated concentration of ZnCl2. The reaction was stopped after two minutes by adding 2 ml of chloroform/methanol solution (2:1).







TABLE 2







Phosphatidylcholine turnover in lca1Δ, slc1Δ and BY4741 (WT) strains










28° C.
37° C.















Chase time (h):
0
1
2
0
1
2


















lca1Δ
Medium
12.1 ± 1.7
16.2 ± 1.1
17.4 ± 2.7
12.1 ± 1.7
13.2 ± 3.5
13.3 ± 1.5



Intracellular
41.1 ± 3.2
47.8 ± 2.8
53.6 ± 1.5
41.1 ± 3.2
67.9 ± 2.7
73.5 ± 2.5



Membrane
46.8 ± 2.8
36.0 ± 2.3
28.9 ± 1.5
46.8 ± 2.8
18.9 ± 3.4
13.2 ± 2.3


slc1Δ
Medium
14.5 ± 1.1
16.1 ± 1.3
17.2 ± 1.6
14.5 ± 0.3
18.3 ± 2.3
19.7 ± 0.7



Intracellular
37.4 ± 2.1
40.1 ± 2.2
42.1 ± 3.3
37.4 ± 1.5
49.1 ± 1.9
57.4 ± 4.1



Membrane
48.1 ± 1.7
43.8 ± 2.6
40.7 ± 1.7
48.1 ± 0.9
32.6 ± 2.8
22.9 ± 1.3


WT
Medium
14.1 ± 0.8
16.1 ± 2.8
17.1 ± 3.1
14.1 ± 0.8
14.9 ± 1.5
15.3 ± 0.5



Intracellular
36.5 ± 0.2
37.1 ± 1.6
41.6 ± 0.1
36.5 ± 0.2
55.9 ± 2.1
60.7 ± 4.3



Membrane
49.4 ± 0.8
46.9 ± 1.2
41.3 ± 3.2
49.4 ± 0.8
29.2 ± 0.6
24.1 ± 2.7









Yeast cells were labeled at starting OD600=1.5 for five hours in chemically defined synthetic medium containing 0.15 μCi/ml. The cells were then washed twice, cultured in medium containing 10 mM non-radioactive choline at 28° C. and 37° C. One microliter culture aliquot was removed, separated into three factions then scintillation counted. The data were presented as mean of three analyses.
Lyso-PAF sensitivity: Yeast strains Y02431 over-expressing LCA1 or harboring empty vector were first grown in 15 ml of SC-ura medium containing 2% glucose then transferred to SC-ura+2% galactose and 1% raffinose. After 12 hours LCA1 expression induction, the culture was diluted to correspond to OD600 value of 0.5, 1, 2, 3, 4. Five μl of each dilution was spotted to a YPD plate supplemented with varying concentrations of Lyso-PAF. The plates were incubated at 28° C. for two days.
PC turnover analysis: PC turnover analysis was performed according to previously described method [13] with slight modification. Briefly, Y02431 and BY4741 yeast cells were grown overnight in chemically defined synthetic media without inositol and choline. Yeast at OD600=1.5 were used to inoculate fresh chemically defined synthetic media containing 0.15 μCi/ml [14C]choline chloride (20 μM). Cells were harvested through centrifugation after 5 hours labeling, washed twice in fresh non-radioactive medium, and then inoculated into in medium containing 10 mM non-radioactive choline. At different time points, 1 ml aliquots were removed and centrifuged. The supernatant was saved as the “medium” fraction. The cell pellet was suspended in 0.5 ml 5% trichloroacetic acid (TCA) and incubated on ice with frequent vortexing. Following centrifugation at 14,000 rpm (Eppendorf), the TCA-containing supernatant was decanted as “intracellular water-soluble fraction”, and neutralized by adding 1 M Tris-HCl (pH 8.0) to avoid acid-induced luminescence in scintillation counting. The pellet was saved as the “membrane” fraction. The labeling of each fraction was measured and presented as percentage of total counts in all the three fractions. To confirm that the majority of choline-containing compounds in TCA fraction are glycerophosphorylcholine (GroPC), the fractions from WT and lca1Δ yeast cells chased for two hours at 37° C. were applied to Merck silica G60 gel and developed in solvent system methanol/0.5% NaCl/NH3.H2O (50/50/1, v/v/v)[14]. After drying, choline-containing chemicals on the plate were detected with scanner (Bioscan, Inc.) and only one major [14C]-labeled spot was clearly detected. The spot was scraped off and re-extracted into distilled water then concentrated with a vacuum refrigerator. The purified TCA fractions were spotted on Merck silica G60 plate with soluble choline-containing compound standards including GroPC, phosphocholine, CDP-choline, [14C] choline and 16:1-LPC, loaded on parallel lanes. The TLC plate was developed in the above-mentioned solvent system. [14C] choline and [14C] choline containing compound in TCA fractions were detected with scanner (Bioscan, Inc.), LPC was stained by iodine exposure, and other choline-containing standards were visualized by spraying molybdenum blue, which is specific to phosphorus present in GroPC, phosphocholine and CDP-choline [15].
Yeast culture: One colony each of wild-type (strain BY4741) and LPCAT mutants (YOR175c deletion strains Y12431, Y02431) are inoculated in 10 ml YPD media and grown overnight. After 24 hours, another 20 ml YPD media is added and growth is continued for another 24 hours.
Protein extraction: Yeast cultures are spun at 2800 rpm at 4° C. for 20 minutes. The supernatant is discarded and the yeast pellet washed with 10 ml of ice cold IB buffer (80 mM HEPES, 320 mM sucrose, 5 mM EDTA pH 8, 10 mM KCl, 2 mM DTT). The pellets are spun again and re-suspend in 500 μl of IB buffer. Yeast cells are divided and transferred into two tubes appropriate for a mini-bead beater. 0.5 mm cold glass beads are added to fill completely the tube. To break the yeast cell, three 60-second pulses of the mini-bead beater are used. The mixtures are spun again to remove unbroken cells and debris.
Protein assay conditions: A reaction is conducted using the recipe for fatty-CoA substrate specificity, as listed in Table 3.








TABLE 3





Solution
Volume added







450 μM lyso-PC (18:1)
50 μl


180 μM 14C 18:1-CoA (10 nCi/nmol)
50 μl


Microsome
400 μg protein


pH 7.4 HEPES
to make fine volume 0.5 ml









The reaction mixture is allowed to sit in a water bath at 30° C. and stirred at 100 rpm for 30 minutes. The reaction is then terminated by adding 2 ml of CH2Cl2: Isopropanol (1:2). The mixture is allowed to sit at room temperature for 15-30 minutes with occasional vortexing. Phases are separated by adding 2 ml CH2Cl2 followed by 2 ml 1M KCl in H3PO4. The lower layer is transferred to a clean tube and the upper aqueous phase is backwashed twice with CH2Cl2 and centrifuged, saving the organic phase each time. Organic phases are combined and dried under nitrogen. Dried material is taken up in 200 μl CH2Cl2: MeOH (2:1) and protein is separated by thin layer chromatography (TLC) using silica G (250 μm) commercial plate. Plates are developed to within 2 cm of top in ethyl acetate:isooctane:acetic acid (45:15:10, V/V/V), then dried and scraped. The phosphatidyl choline region is counted in 4 ml Aquasol-2 by a scintillation counter.
The YOR175c gene from S. cerevisiae has been identified as encoding an LPC acyltransferase. The coding sequence of this yeast LPC acyltransferase gene is SEQ ID NO:1:








ATGTACAATCCTGTGGACGCTGTTTTAACAAAGATAATTACCAACTATGGGATTGATAGT



 


TTTACACTGCGATATGCTATCTGCTTATTGGGATCGTTCCCACTGAATGCTATTTTGAAG


 


AGAATTCCCGAGAAGCGTATAGGTTTAAAATGTTGTTTTATCATTTCTATGTCGATGTTT


 


TACTTATTCGGTGTGCTGAATCTAGTAAGTGGATTCAGGACCCTGTTTATTAGTACCATG


 


TTTACTTACTTGATCTCAAGATTTTACCGTTCCAAGTTTATGCCACACTTGAATTTCATG


 


TTTGTTATGGGTCATTTGGCAATAAATCATATACACGCCCAATTCCTTAACGAACAGACT


 


CAAACTACCGTTGACATTACAAGTTCACAAATGGTTTTAGCCATGAAACTAACTTCTTTT


 


GCATGGTCGTACTATGATGGTTCATGCACTAGCGAAAGCGATTTCAAAGATTTGACTGAG


 


CATCAAAAATCTCGTGCTGTCAGAGGTCATCCACCCTTATTAAAGTTCCTGGCATATGCA


 


TTTTTCTATTCAACGTTGCTAACTGGCCCAAGTTTCGATTATGCCGATTTTGACAGCTGG


 


TTGAATTGTGAGATGTTCCGTGACTTGCCTGAAAGCAAAAAGCCTATGAGAAGACACCAC


 


CCTGGTGAAAGAAGACAGATTCCAAAGAATGGTAAACTTGCATTATGGAAAGTTGTTCAA


 


GGTCTTGCTTGGATGATTTTAAGTACACTAGGAATGAAGCACTTCCCCGTAAAATACGTT


 


TTGGACAAAGATGGCTTCCCAACGAGATCTTTTATATTCAGAATCCATTACTTATTCTTG


 


CTTGGTTTCATCCATAGATTCAAGTACTACGCTGCCTGGACTATTTCGGAAGGATCTTGT


 


ATTTTGTGCGGTTTGGGTTATAATGGTTATGATTCAAAGACACAAAAGATCAGATGGGAT


 


CGTGTCAGAAATATTGACATTTGGACCGTAGAAACGGCGCAGAATACGCGTGAAATGTTG


 


GAAGCATGGAATATGAATACTAACAAGTGGCTAAAATACTCTGTTTATTTACGTGTCACA


 


AAGAAGGGCAAAAAACCTGGTTTCCGCTCAACTTTGTTTACTTTCCTAACTTCCGCATTT


 


TGGCATGGTACCAGACCTGGGTACTATCTGACTTTTGCGACAGGGGCTTTGTACCAAACA


 


TGTGGTAAAATCTACAGACGCAATTTTAGACCAATTTTCTTGCGAGAAGATGGTGTCACT


 


CCTTTGCCTTCTAAAAAAATCTACGATTTAGTTGGCATATATGCAATTAAACTAGCATTT


 


GGTTACATGGTGCAACCATTTATTATCCTTGATTTGAAGCCATCTTTAATGGTATGGGGC


 


TCTGTTTATTTCTATGTTCATATTATTGTTGCTTTCTCATTTTTCCTATTCAGAGGACCA


 


TATGCTAAACAAGTTACTGAATTTTTTAAATCCAAACAACCTAAAGAAATATTCATTAGA


 


AAACAAAAGAAGTTGGAAAAAGATATTTCTGCAAGCTCTCCAAACTTGGGTGGTATATTG


 


AAGGCAAAGATTGAACATGAAAAGGGAAAGACAGCAGAAGAAGAAGAAATGAACTTAGGT


 


ATTCCACCAATTGAGTTAGAAAAGTGGGACAATGCTAAGGAAGATTGGGAAGATTICTGC


 


AAAGATTACAAAGAATGGAGAAATAAAAATGGTCTTGAAATAGAAGAGGAAAACCTTTCT


 


AAAGCTTTTGAAAGATTCAAGCAGGAATTTTCTAACGCTGCAAGTGGATCAGGTGAACGT


 


GTGAGAAAAATGAGTTTTAGTGGTTACTCACCAAAGCCTATTTCAAAAAAGGAAGAGTAG






The deduced amino acid sequence of the yeast LPC acyltransferase encoded by the gene is SEQ ID NO:2:








MYNPVDAVLTKIITNYGIDSFTLRYAICLLGSFPLNAILKRIPEKRIGLKCCFIISMSMF



 


YLFGVLNLVSGFRTLFISTMFTYLISRFYRSKFMPHLNFMFVMGHLAINHIHAQFLNEQT


 


QTTVDITSSQMVLAMKLTSFAWSYYDGSCTSESDFKDLTEHQKSRAVRGHPPLLKFLAYA


 


FFYSTLLTGPSFDYADFDSWLNCEMFRDLPESKKPMRRHHPGERRQIPKNGKLALWKVVQ


 


GLAWMILSTLGMKHFPVKYVLDKDGFPTRSFIFRIHYLFLLGFIHRFKYYAAWTISEGSC


 


ILCGLGYNGYDSKTQKIRWDRVRNIDIWTVETAQNTREMLEAWNMNTNKWLKYSVYLRVT


 


KKGKKPGFRSTLFTFLTSAFWHGTRPGYYLTFATGALYQTCGKIYRRNFRPIFLREDGVT


 


PLPSKKIYDLVGIYAIKLAFGYMVQPFIILDLKPSLMVWGSVYFYVHIIVAFSFFLFRGP


 


YAKQVTEFFKSKQPKEIFIRKQKKLEKDISASSPNLGGILKAKIEHEKGKTAEEEEMNLG


 


IPPIELEKWDNAKEDWEDFCKDYKEWRNKNGLEIEEENLSKAFERFKQEFSNAASGSGER


 


VRKMSFSGYSPKPISKKEE






Deletion of YOR175cp leads to reduced lysophosphatidylcholine acyltransferase (LPCAT) activity: YOR175c is a MBOAT family protein, and was shown to be localized in endoplasm reticulum. In a preliminary experiment, we first examined if disruption of YOR175c would have any impact on lysophosphatidic acid acyltransferase (LPAAT) and LPCAT activities using both the parental strain and slc1Δ mutant as controls. When lysophosphatidic acid (18:1) was supplied as acyl acceptor, the cell lysate of slc1Δ mutant had a LPAAT level reduced to 63% of the parental strain, but we detected no significant LPAAT reduction in the yor175cΔ mutant. In marked contrast, when LPC was provided as acyl acceptor, our in vitro assay showed acyltransferase activity reduction in yor175cΔ to a level approximately 28% of the parental strain. The slc1Δ displayed no significant decrease in LPCAT activity as compared with WT strain (FIG. 9). We further investigated sn-2 lysophospholipid acyl transferase activity in yor175cΔ by using microsomal enriched fractions with different lysophospholipid acyl acceptors and palmitoyl-CoA (16:0-CoA). In keeping with the results of total cell lysate, microsomal fractions of the yor175cΔ strain showed a striking decrease in LPCAT activity. LPE and LPG acyltransferase were also slightly decreased, but to a much lesser degree (FIG. 10).
YOR175cp displays in vitro acyltransferase activity with preference for LPC: Microsomal preparations of lca1Δ mutant expressing YOR175c and lca1Δ harboring the empty vector (VO) were used to perform acyltransferase assays with [14C] palmitoyl-CoA and various lysophospholipids substrates including LPA, LPC, LPE, LPG, LPI and LPS. As shown in FIG. 11, the highest activity was found with LPC as substrate. The activity of LPC acylation was linear at 30° C. for 20 minutes, and the conversion of LPC to PC is negligible in the absence of 16:0-CoA (data not shown). Over-expression of YOR175c also caused substantial increases in the acylation of LPG and LPE. But the rates of LPG and LPE acylation were at a level approximately 60% and 20%, respectively, of the activity registered for LPC. Activities for LPA, LPS and LPI, were all less than 1% of the activity of LPCAT. Thus, YOR175c appeared capable of accepting several major lysophospholipid classes, but under our assay conditions it exhibited the highest activity with LPC.
Correlation of YOR175c LPCAT activity with Lyso-PAF sensitivity: Although not an endogenous acyl acceptor, ether-linked glycerolipid, lyso-PAF, can be acylated in yeast, and the reaction was attributed to a LPCAT. When lyso-PAF was used as acyl acceptor, the lca1Δ strain had a rate of lyso-PAF acylation reduced to 31.1% of WT strain. Conversely, over-expression of LCA1 resulted in 86.3-fold increase in lyso-PAF acyltransferase activity. It was established previously that high lyso-PAF level exerts toxic effect on yeast cells. Consistent with in vitro results, LCA1 mediating Lyso-PAF acylation was also evident in a plate assay (FIG. 12). In our study, both the parental strain and the lca1Δ were capable of tolerating LPC at a level up to 20 μg/ml, but the lca1Δ mutant displayed hypersensitivity to lyso-PAF at a concentration above 5 μg/ml. Moreover, its sensitivity to lyso-PAF was ameliorated by the expression of LCA1. In contrast, slc1Δ A strain could survive and grew well on lyso-PAF plate without any apparent difference from WT cells, indicating SLC1 disruption did not affect lyso-PAF acylation.
Zn2+ inhibitory effect on LPCAT activity: Zn2+ caused significant reduction of LPCAT activity of LCA1 in a range between 0.1 mM to 20 mM (Table 1). Our results also suggested that a lower (10-25 μM) concentration of Zn2+ enhanced LPCAT activity. The maximum increase was observed with 25 μM ZnCl2. We did not detect significant effect of Mg2+ on LPCAT activity of LCA1, in a concentration ranging from 5 to 40 μM (data not shown).
Kinetic parameters of LCA1: Kinetics constants based on Lineweaver-Burk double-reciprocal plot analysis showed that LCA1 had an apparent Km for acyl-CoA at 0.89±0.25 μM and a Vmax of 524 pmol/min/μg protein. PC molecules are distinguished by fatty acid chain length. As shown in FIG. 13 (in graph A), LCA1 exhibited a LPC substrate preference in the order of oleic (18:1)-LPC>stearic (18:0)-LPC>palmitic (16:0)-LPC. The fatty acid substrate specificity of the LCA1 was also assessed using acyl-CoA with chain lengths ranging from 14 to 22 carbons. Based on assays using 50 μM acyl-CoAs, LCA1 could use a broad range of acyl-CoAs (FIG. 13, in graph B), but it displayed particularly high activities with 16:0-CoA, 18:0-CoA and 18:1-CoA, regardless whether 18:1-LPC or 16:0-LPC was used as acyl acceptor. Interestingly, LCA1 could also efficiently mediate LPC acylation using very long chain fatty acyl-CoAs, such as 20:0-CoA and 22:6-CoA. LPC (16:0) at concentrations above 75 μM, and acyl-CoA at higher than 10 μM, exerted inhibitory effects on LPCAT activity (data not shown).
LCA1 is involved in PC turnover: We studied PC turnover by following an established protocol [13]. We included a slc1Δ strain in the PC turnover analysis in order to differentiate the involvement of SLC1 and LCA1. The yeast cells were cultured and labeled in chemically defined synthetic medium containing [14C] choline at 28° C. Because higher growth temperature particularly accelerates the deacylation process [13], [14C] choline was subsequently chased by 10 mM exogenously added choline at 28° C. and 37° C., respectively. The 14C labels in the membrane fraction, intracellular non-membrane fraction, and in the medium were monitored at different time points. There was no significant difference with regard to the dynamics of membrane-associated labels between slc1Δ and WT. Each lost about 8% at 28° C., and 25% at 37° C. of labeling, in the membrane fraction over the course of 2 hours. In contrast, the lca1Δ strain lost 18% at 28° C., and 33% at 37° C., over the same period of time. The label was rising in the intracellular, non-membrane fraction, which was suggested to be of mainly glycerophosphorylcholine (GroPC) [13, 24], a product of PC deacylation. We attempted to separate the compounds in TCA fraction on Merck silica G60 plate and found only one [14C]-choline band detected. We then purified the choline-containing compound in TCA fractions and developed on the same TLC plate with commercial choline-containing chemical standards. The compound clearly showed the same migration rate as GroPC (FIG. 14). Since an increased GroPC level was observed in both 28° C. and 37° C., these results suggested that the metabolic impact was independent of PC deacylation, therefore strongly suggesting that lca1Δ was compromised in the reacylation process of the Lands' cycle. That slc1Δ had a similar PC turnover rate to that of the WT strain indicated that, although being a major sn-2 acyltransferase, SLC1 did not appear to play a significant role in PC turnover.
Example 2
Identification of Plant Nucleotide Sequences Encoding LPC Acyltransferase
The nucleotide sequence of the yeast LPC acyltransferase gene was used to search for homologous sequences using computer programs designed to search for homologous sequences. For instance, readily commercially available computer programs that may be used for such searches include without limitation, BLASTN, BLASTX and TBLASTX which may be used to search for nucleotide sequences, and BLASTP and TBLASTN which may be used to search for amino acid sequences. Such computer programs are readily accessible at the web-site WorldWideWeb.ncbi.nlm.nih.gov.
Two plant (A. thaliana) homologs were identified through sequence alignment searching using BLAST. The two homologs are cDNA sequences that encode two different isoforms of LPC acyltransferase.
Arabidopsis LPC Acyltransferase 1
Nucleotide sequence of Arabidopsis LPC acyltransferase 1 cDNA is SEQ ID NO:3:









1
ACCAACAACC ACACGACACG ACACGACCGA TCTATAGATT CGGCGAGATC



 


51
AGAAGAAAGC TTCCCGGAGC AACTCGGTCG TTGTGACTCA TTCCGAGTTA


 


101
AAAAAAACGG GTTTTCGACA CCATGGATAT GAGTTCAATG GCTGGTTCAA


 


151
TCGGAGTTTC GGTAGCCGTA CTCCGATTCC TCCTCTGTTT CGTTGCCACG


 


201
ATCCCTGTTT CATTCGCTTG TCGAATCGTC CCGAGTAGAC TCGGTAAACA


 


251
CTTGTATGCC GCTGCTTCAG GTGCTTTCCT CTCTTACCTC TCCTTTGGCT


 


301
TCTCCTCCAA CCTTCACTTC CTTGTTCCGA TGACGATCGG ATATGCTTCA


 


351
ATGGCGATTT ATAGACCCAA GTGTGGAATC ATCACTTTCT TCCTCGGTTT


 


401
CGCTTATCTT ATTGGCTGTC ATGTGTTTTA TATGAGTGGT GATGCGTGGA


 


451
AAGAAGGAGG AATCGATTCT ACTGGAGCGT TAATGGTGTT GACGCTGAAA


 


501
GTCATCTCAT GTTCAATGAA TTACAATGAT GGGATGTTGA AGGAGGAAGG


 


551
TCTACGTGAA GCTCAGAAGA AAAACAGATT GATTCAGATG CCGTCTTTGA


 


601
TTGAGTACTT TGGTTACTGC CTTTGTTGTG GTAGCCATTT TGCTGGTCCT


 


651
GTTTATGAAA TGAAAGATTA TCTTGAATGG ACCGAAGGGA AAGGGATTTG


 


701
GGATACTACT GAGAAAAGAA AGAAGCCATC GCCTTATGGA GCTACAATCC


 


751
GAGCTATTTT GCAAGCTGCG ATTTGCATGG CTCTGTATCT CTATTTAGTG


 


801
CCTCAATATC CGTTAACTCG GTTCACAGAA CCAGTGTATC AAGAATGGGG


 


851
ATTCTTGAGA AAATTTAGTT ACCAATACAT GGCTGGATTC ACGGCTCGTT


 


901
GGAAGTATTA CTTCATCTGG TCAATTTCAG AGGCTTCTAT TATCATCTCT


 


951
GGTTTGGGTT TCAGTGGTTG GACTGATGAT GCTTCACCAA AGCCCAAATG


 


1001
GGACCGTGCC AAGAACGTAG ATATTCTCGG TGTTGAACTA GCTAAGAGCG


 


1051
CGGTTCAGAT TCCACTTGTG TGGAACATAC AAGTCAGCAC GTGGCTCCGT


 


1101
CACTATGTGT ATGAGAGACT TGTGCAGAAC GGAAAGAAAG CGGGTTTCTT


 


1151
CCAGTTACTA GCTACACAAA CCGTCAGCGC GGTTTGGCAT GGACTGTATC


 


1201
CTGGATATAT GATGTTCTTT GTTCAGTCAG CTTTGATGAT CGCAGGCTCA


 


1251
CGGGTTATTT ACCGGTGGCA ACAAGCGATC AGTCCGAAAA TGGCAATGCT


 


1301
GAGAAATATA ATGGTCTTCA TCAACTTCCT TTACACTGTT TTGGTTCTCA


 


1351
ACTACTCAGC CGTCGGTTTC ATGGTGTTAA GCTTGCACGA AACACTTACC


 


1401
GCCTACGGAA GCGTATATTA CATTGGAACA ATCATACCTG TTGGATTGAT


 


1451
TCTCCTCAGT TACGTTGTGC CTGCAAAACC TTCAAGACCA AAACCGCGTA


 


1501
AAGAAGAATA AGCAGTTATC TTCTTCTCTT AACGGTAAGT AAGTTTCCCG


 


1551
CGCTTGCCAG CTTCTTCTTC TTCTTCTGTA ACATTTGGAA ACAAACCGAT


 


1601
CCGGTTCTTG TTTCTCTCTG ATTTTTTAGC ACCGATATTT TTTTTGTATT


 


1651
TGTTGCTTAT AAATCTTATT TTTCACACTT CTTTTTTTTA ATTAGTATTG


 


1701
GATTTGCAAT TATATAGACA ATAAGTATAA ATATGTAACT GTAAATTGCA


 


1751
AATGGGAAAA AATAGTAGTG TTTATGTTTG






The deduced amino acid sequence of Arabidopsis LPC acyltransferase 1 is SEQ ID NO:4:









1
MDMSSMAGSI GVSVAVLRFL LCFVATIPVS FACRIVPSRL GKHLYAAASG



 


51
AFLSYLSFGF SSNLHFLVPM TIGYASMAIY RPKCGIITFF LGFAYLIGCH


 


101
VFYMSGDAWK EGGIDSTGAL MVLTLKVISC SMNYNDGMLK EEGLREAQKK


 


151
NRLIQMPSLI EYFGYCLCCG SHFAGPVYEM KDYLEWTEGK GIWDTTEKRK


 


201
KPSPYGATIR AILQAAICMA LYLYLVPQYP LTRFTEPVYQ EWGFLRKFSY


 


251
QYMAGFTARW KYYFIWSISE ASIIISGLGF SGWTDDASPK PKWDRAKNVD


 


301
ILGVELAKSA VQIPLVWNIQ VSTWLRHYVY ERLVQNGKKA GFFQLLATQT


 


351
VSAVWHGLYP GYMMFFVQSA LMIAGSRVIY RWQQAISPKM AMLRNIMVFI


 


401
NFLYTVLVLN YSAVGFMVLS LHETLTAYGS VYYIGTIIPV GLILLSYVVP


 


451
AKPSRPKPRK EE






Arabidopsis LPC Acyltransferase 2
Nucleotide sequence of Arabidopsis LPC acyltransferase 2 cDNA is SEQ ID NO:5:









1
AGATGTCCGA ACTGTGAGAG TCGTCGTCGT CGTCGTAACT CAGTCCGAGT



 


51
TGACACAATC TTCCACTTCA CGCAAGATAC AACCATGGAA TTGCTTGACA


 


101
TGAACTCAAT GGCTGCCTCA ATCGGCGTCT CCGTCGCCGT TCTCCGTTTC


 


151
CTCCTCTGTT TCGTCGCAAC GATACCAATC TCATTTTTAT GGCGATTCAT


 


201
CCCGAGTCGA CTCGGTAAAC ACATATACTC AGCTGCTTCT GGAGCTTTCC


 


251
TCTCTTATCT CTCCTTTGGC TTCTCCTCAA ATCTTCACTT CCTTGTCCCA


 


301
ATGACGATTG GTTACGCTTC AATGGCGATT TATCGACCCT TGTCTGGATT


 


351
CATTACTTTC TTCCTAGGCT TCGCTTATCT CATTGGCTGT CATGTGTTTT


 


401
ATATGAGTGG TGATGCTTGG AAAGAAGGAG GAATTGATTC TACTGGAGCT


 


451
TTGATGGTAT TAACACTGAA AGTGATTTCG TGTTCGATAA ACTACAACGA


 


501
TGGAATGTTG AAAGAAGGAG GTCTACGTGA GGCTCAGAAG AAGAACCGTT


 


551
TGATTCAGAT GCCTTCTCTT ATTGAGTACT TTGGTTATTG CCTCTGTTGT


 


601
GGAAGCCATT TCGCTGGCCC GGTTTTCGAA ATGAAAGATT ATCTCGAATG


 


651
GACTGAAGAG AAAGGAATTT GGGCTGTTTC TGAAAAAGGA AAGAGACCAT


 


701
CGCCTTATGG AGCAATGATT CGAGCTGTGT TTCAAGCTGC GATTTGTATG


 


751
GCTCTCTATC TCTATTTAGT ACCTCAGTTT CCGTTAACTC GGTTCACTGA


 


801
ACCAGTGTAC CAAGAATGGG GATTCTCGAA GAGATTTGGT TACCAATACA


 


851
TGGCGGGTTT CACGGCTCGT TGGAAGTATT ACTTTATATG GTCTATCTCA


 


901
GAGGCTTCTA TTATTATCTC TGGTTTGGGT TTCAGTGGTT GGACTGATGA


 


951
AACTCAGACA AAGGCTAAAT GGGACCGCGC TAAGAATGTC GATATTTTGG


 


1001
GGGTTGAGCT TGCCAAGAGT GCGGTTCAGA TTCCGCTTTT CTGGAACATA


 


1051
CAAGTCAGCA CATGGCTCCG TCACTACGTA TATGAGAGAA TTGTGAAGCC


 


1101
CGGGAAGAAA GCGGGTTTCT TCCAATTGCT AGCTACGCAA ACCGTCAGTG


 


1151
CTGTCTGGCA TGGACTGTAT CCTGGATACA TTATATTCTT TGTGCAATCA


 


1201
GCATTGATGA TCGATGGTTC GAAAGCTATT TACCGGTGGC AACAAGCAAT


 


1251
ACCTCCGAAA ATGGCAATGC TGAGAAATGT TTTGGTTCTC ATCAATTTCC


 


1301
TCTACACAGT AGTGGTTCTC AATTACTCAT CCGTCGGTTT CATGGTTTTA


 


1351
AGCTTGCACG AAACACTAGT CGCCTTCAAG AGTGTATATT ACATTGGAAC


 


1401
AGTTATACCT ATCGCTGTGC TTCTTCTCAG CTACTTAGTT CCTGTGAAGC


 


1451
CTGTTAGACC AAAGACCAGA AAAGAAGAAT AATGTTGTCT TTTTAAAAAA


 


1501
TCAACAACAT TTTGGTTCTT TTCTTTTTTT CCACTTGGAC CGTTTTATGT


 


1551
AAAACAAGAG AAATCAAGAT TTGAGGTTTT ATTCTTCTTC TCCTTCCCAA


 


1601
TTTTCGAAAA TGATTTTATT TTTTCTGATA TATATCTAAG CTAGTCCAAA


 


1651
GTCAACTCG






The deduced amino acid sequence of Arabidopsis LPC acyltransferase 2 is SEQ ID NO:6:









1
MELLDMNSMA ASIGVSVAVL RFLLCFVATI PISFLWRFIP SRLGKHIYSA



 


51
ASGAFLSYLS FGFSSNLHFL VPMTIGYASM AIYRPLSGFI TFFLGFAYLI


 


101
GCHVFYMSGD AWKEGGIDST GALMVLTLKV ISCSINYNDG MLKEEGLREA


 


151
QKKNRLIQMP SLIEYFGYCL CCGSHFAGPV FEMKDYLEWT EEKGIWAVSE


 


201
KGKRPSPYGA MIRAVFQAAI CMALYLYLVP QFPLTRFTEP VYQEWGFLKR


 


251
FGYQYMAGFT ARWKYYFIWS ISEASIIISG LGFSGWTDET QTKAKWDRAK


 


301
NVDILGVELA KSAVQIPLFW NIQVSTWLRH YVYERIVKPG KKAGFFQLLA


 


351
TQTVSAVWHG LYPGYIIFFV QSALMIDGSK AIYRWQQAIP PKMAMLRNVL


 


401
VLINFLYTVV VLNYSSVGFM VLSLHETLVA FKSVYYIGTV IPIAVLLLSY


 


451
LVPVKPVRPK TRKEE






AtLPCAT1 and AtLPCAT2 lysophospholipid acyltransferase activity was in vitro assessed with various lysophospholipid including lysophosphatidic acid (“LPA”), lysophosphatidylcholine (“LPC”), lysophosphatidylethanolamine (“LPE”), lysophosphatidylinositol (“LPI”), lysophosphatidylglycerol (“LPG”), lyso-platelet-activating factor as acyl group acceptor and [14C]-palmitoyl-CoA as acyl group donor. Results clearly showed that lysophosphatidylcholine and lyso-platelet-activating factor were the most preferred lysophospholipid substrates (FIG. 15). Preference of LPCAT1 and LPCAT2 towards lyso-platelet-activating factor was also evidenced by lyso-PAF plate test (FIG. 16).
Acyl-CoA preference of AtLPCAT1 and AtLPCAT2 was assessed in vitro with various acyl-CoA species as acyl-group donor and [16C]-palmitoyl-sn2-lysophosphatidylcholine as acyl-group acceptor. AtLPCAT1 preferred monounsaturated 16:1 and 18:1-acyl-CoA followed by 16:0 and 18:0-acyl-CoA. AtLPCAT2 similarly preferred 16:0, 16:1, 18:0 and 18:1-CoA. Comparatively, both AtLPCATs discriminated against 18:2 acyl-CoA as acyl group donor (FIG. 17).
AtLPCAT1 and AtLPCAT2 preferences towards LPC species of different chain length were in vitro assessed with lysophosphatidylcholine of various chain lengths as acyl-group acceptor and [14C]-palmitoyl-CoA as acyl-group donor. AtLPCAT1 and AtLPCAT2 both preferred 16:0 and 18:1-lysophosphatidylcholine (FIG. 18).
Zn2+ sensitivity of AtLPCATs was investigated, activities of both AtLPCATs decreased with increasing concentration of Zn2+ added into in vitro assay reactions (FIG. 19).
Example 3
Transformation of a Plant with LPC Acyltransferase Gene
Transformation protocol is adapted from that described by Bechtold et al. (1993). Plants are grown in moist soil at a density of 10-12 plants per pot, in 4-inch square pots, and are covered with a nylon screen fixed in place with an elastic band. When the plants reach the stage at which bolts emerge, plants are watered, the bolts and some of the leaves are clipped, and the plants are infiltrated in Agrobacterium suspension as outlined below.
Agrobacterium transformed with the LPC acyltransferase gene of the instant invention is grown in a 25 mL suspension in LB medium containing kanamycin at a concentration of 50 ng/mL. The Agrobacterium is cultured for two to three days. The day before infiltration, this “seed culture” is added to 400 mL of LB medium containing 50 ng/mL kanamycin. When the absorbance at 600 nm is >2.0, the cells are harvested by centrifugation (5,000 times g, ten minutes in a GSA rotor at room temperature) and are re-suspended in 3 volumes of infiltration medium (one times Murashige and Skoog salts, one times, B5 vitamins, 5.0% sucrose, 0.044 μM benzylaminopurine) to an optical density at 600 nm of 0.8. The Agrobacterium suspension is poured into a beaker and the potted plants are inverted into the beaker so that the bolts and entire rosettes are submerged. The beaker is placed into a large Bell jar and a vacuum is drawn using a vacuum pump, until bubbles form on the leaf and stem surfaces and the solution starts to bubble a bit, and the vacuum is rapidly released. The necessary time and pressure vanes from one lab setup to the next; but good infiltration is visibly apparent as uniformly darkened, water-soaked tissue. Pots are removed from the beaker, are laid on their side in a plastic tray and are covered with a plastic dome, to maintain humidity. The following day, the plants are uncovered, set upright and are allowed to grow for approximately four weeks in a growth chamber under continuous light conditions as described by Katavic et al. (1995). When the siliques are mature and dry, seeds are harvested and selected for positive transformants.
Example 4
Selection of Putative Transformants (Transgenic Plants) and Growth and Analysis of Transgenic Plants
Seeds are harvested from vacuum-infiltration transformation procedures, and are sterilized by treating for one minute in ethanol and five minutes in 50% bleach/0.05% TweenTM 20TM in sterile distilled water. The seeds are rinsed several times with sterile distilled water. Seeds are plated by re-suspending them in sterile 0.1% agarose at room temperature (about 1 mL agarose for every 500-1000 seeds), and applying a volume equivalent to about 2,000-4,000 seeds onto 150×15 mm selection plates (1⁄2×Murashige and Skoog salts, 0.8% agar, autoclave, cool and add 1×B5 vitamins and kanamycin at a final concentration of 50 μg/mL). The plates are dried in a laminar flow hood until seed no longer flows when the plates are tipped. The plates are vernalized for two nights at 4° C. in the dark, and are moved to a growth chamber (conditions as described by Katavic et al., 1995). After seven to ten days, transformants are clearly identifiable as dark green plants with healthy green secondary leaves and roots that extend over and into the selective medium.
Seedlings are transplanted to soil, plants are grown to maturity and mature seeds (T2 generation as defined in Katavic et al., 1994) are collected and analyzed. T2 seeds are propagated. The vegetative growth patterns are monitored by measuring shoot tissue dry weights, and/or by counting the number of rosette leaves present by the time plants began to enter the generative (flower initiation) stage. Floral initiation (beginning of generative phase of growth) is analyzed by recording, on a daily basis, the percentage of plants in which a flower bud first appears and/or the percentage of plants that are bolting (as described by Zhang et al., 1997). Data are reported in terms of percentage of plants flowering/bolting on a given day after planting (d.a.p.).
Example 5
Analysis of Fatty Acids
Cells or plants transformed with the LPC acyltransferase gene of the instant invention are grown to maturity and mature seeds are harvested. Fatty acids are extracted from the cells or plants transformed with the LPC acyltransferase gene. Normal-phase HPLC analysis is used to assay for the production of fatty acids in the transformed cells or plants.
Example 6
Analysis of LPCAT from Various Species
(1) Identification of LPCAT from the Alga Thalassiosira pseudonana 
We made use of the sequence information of LPCAT from S. cerevisiae (SEQ ID NO:1) and identified a sequence coding for LPCAT from the alga T. pseudonana. This algal LPCAT shows 27% identity at the amino acid to the yeast LPCAT which is encoded by YOR175c.
The nucleotide and amino acid sequences of LPCAT from T. pseudonana 
(a) The nucleotide sequence of LPCAT from the alga T. pseudonana








(SEQ ID NO: 7)



ATGCGATTGTATTTGCAATTCAACTTATCCATCAATGATTATTGTCACTTCTTC



 


ACAGTACCATCCTTTGTCAAAGAGGGCGTCGAGTCTCTCTCTGCATCCACCGGACAA


 


GACGTCGAGACTCTCGAGTACCTCCTTGGTATGCTCATCTGCTACCCCCTCGGAATG


 


ATCATGCTCGCTCTACCCTACGGAAAAGTAAAACATCTCTTCTCCTTCATCCTCGGA


 


GCCTTCCTACTTCAATTCACCATTGGTATCCAGTGGATTCATCACTTAATCTCCTCAA


 


TGATTGCCTACGTCATGTTCCTCGTCCTTCCTGCCAAATTTGCCAAAACGGCAGTGCC


 


TGTGTTTGCCATGATCTACATCACCGCGGGACATTTGCATCGTCAATACATCAATTAT


 


CTTGGGTGGGATATGGACTTCACGGGGCCTCAGATGGTGCTTACGATGAAACTCTAC


 


ATGCTTGCTTACAACCTTGCGGATGGGGACTTGCTCAAGAAGGGAAAGGAGGATAG


 


GGCTGCAAAGAAGTGTGCGGATGTCGCTATTTCGTCTGTTCCCGGAATCATTGAGTA


 


CTTGGGCTACACGTTCTGCTTTGCCAGTGTTTTAGCAGGCCCTGCTTTTGAGTACAAA


 


TTCTACGCCGATGCATGCGACGGATCACTCTTGTACGACAAATCTGGCAAACCCAAA


 


GGAAAGATCCCCAGTCAGGTGTGGCCTACATTGCGTCCTCTTTTTGGAAGTCTCTTGT


 


GTCTCGGCATCTTTGTTGTGGGAACTGGAATGTATCCTCTTTTGGATCCCAACGATCC


 


TCAGAATGCCACTCCTATCCCTCTCACTCCAGAGATGTTGGCCAAACCAGCCTATGC


 


TCGATACGCTTACTCGTGGCTTGCACTCTTTTTCATCCGATTTAAGTATTACTTTGCTT


 


GGATGAACGCCGAAGGAGCAAGCAACATTTGGTATGCTGGATTTGAGGGATTTGAT


 


GCCAGCGGCAACCCCAAAGGATGGGAGGTATCCAATAACATTGACGTAATTCAGTT


 


CGAGACTGCACCCAATCTCAAGACTTTGAGTGCTGCTTGGAATAAGAAGACTGCGA


 


ACTGGTTGGCGAAGTATGTGTACATTCGCACGGGTGGTTCTCTCTTTGCGACGTACG


 


GAATGAGTGCTTTCTGGCATGGCTTCTACCCTGGATACTACCTCTTCTTCATGTCGGT


 


ACCCATGATGGCTTTCTGTGAGAGGATTGGAAGGAAGAAACTTACACCTCGTTTCGG


 


AAATGGAAAGAAGTGGAGTCCTTATGGCATTGTGTGCATTATCGCCACATCGTTGAT


 


GACGGAATACATGATTCAGCCATTCCAACTACTTGCGTTTGATTGGGCCTGGGAGAA


 


CTGGAGCAGCTACTACTTTGCTGGACACATTGTTTGTGTTGTGTTTTACCTCGTTGTG


 


TCCAACATGCCTACACCAAAGACGAAGGAGACTTAA






(b) The amino acid sequence of LPCAT from T. pseudonana








(SEQ ID NO: 8)



MRLYLQFNLSINDYCHFFTVPSFVKEGVESLSASTGQDVETLEYLLGMLICYPLG



 


MIMLALPYGKVKHLFSFILGAFLLQFTIGIQWIHHLISSMIAYVMFLVLPAKFAKTAVPVF


 


AMIYITAGHLHRQYINYLGWDMDFTGPQMVLTMKLYMLAYNLADGDLLKKGKEDRA


 


AKKCADVAISSVPGIIEYLGYTFCFASVLAGPAFEYKFYADACDGSLLYDKSGKPKGKIP


 


SQVWPTLRPLFGSLLCLGIFVVGTGMYPLLDPNDPQNATPIPLTPEMLAKPAYARYAYS


 


WLALFFIRFKYYFAWMNAEGASNIWYAGFEGFDASGNPKGWEVSNNIDVIQFETAPNL


 


KTLSAAWNKKTANWLAKYVYIRTGGSLFATYGMSAFWHGFYPGYYLFFMSVPMMAF


 


CERIGRKKLTPRFGNGKKWSPYGIVCIIATSLMTEYMIQPFQLLAFDWAWENWSSYYFA


 


GHIVCVVFYLVVSNMPTPKTKET






(2) Identification of LPCAT from Diverse Plant Species
Taking the same approach as described above, identified were the full-length or partial sequences of LPCAT from various plant species, including apple, barley, Capsicum annuum, castor bean, grapevine, maize, peach, rice, tomato, snapdragon, sorghum, sunflower, vaccinium corymbosum and wheat as well as Arabidopsis. 
(a) The partial nucleotide sequence of LPCAT from apple







(SEQ ID NO: 9)


TCAGGAGGCCCAAATTTCCTTTGTCAAGATTTACTGAGCCCATATACCA


 


AGAATGGGGGTTTTGGAAACGACTTTTCTACCAGTATATGTCTGGATTC


 


ACAGCAAGGTGGAAATATTATTTCATTTGGTCAATATCAGAGGCTTCTA


 


TCATTCTTTCTGGCCTCGGTTTCAGTGGCTGGACAGAGTCCTCACCACC


 


AAAACCTCGATGGGATCGTGCAAAAAATGTTGATATTATAGGCGTTGAG


 


TTTGCAAAGAGTTCAGTTCAGTTACCACTTGTTTGGAACATACAAGTCA


 


GCACCTGGCTTCGCCATTATGTTTATGATAGGCTTGTTAAACCTGGAAA


 


GAAGCCTGGTTTCTTCCAGTTGCTGGCTACACAGACCGTCAGTGCTGTT


 


TGGCATGGCCTCTATCCTGGCTACATCATATTCTTTGTTCAGTCAGCGT


 


TGATGATTGCTGGATCAAGAGTGATTTACCGATGGCAGCAAGCTGTACC


 


TCCAACTATGGATGTTGTTAAGAAGATATTGGTGTTCATCAACTTTGCT


 


TACACTGTCTTGGTTCTGAACTACTCCTGTGTTGGTTTCATTGTATTAA


 


GCCTTCGTGAAACACTGGCCTCGTATGGAAGCGTGCATTTC






The partial amino acid sequence of LPCAT from apple







(SEQ ID NO: 10)


RRPKFPLSRFTEPIYQEWGFWKRLFYQYMSGFTARWKYYFIWSISEASI


 


ILSGLGFSGWTESSPPKPRWDRAKNVDIIGVEFAKSSVQLPLVWNIQVS


 


TWLRHYVYDRLVKPGKKPGFFQLLATQTVSAVWHGLYPGYIIFFVQSAL


 


MIAGSRVIYRWQQAVPPTMDVVKKILVFINFAYTVLVLNYSCVGFIVLS


 


LRETLASYGSVHF






(b) The partial amino acid sequence of LPCAT from barley







(SEQ ID NO: 11)


EAAIIISGLGFTGWSDSSPPKAKWDRAINVDILGVELAGSAAQLPLKWN


 


IQVSTWLRYYVYERLIQKGKKPGFLQLLGTQTVSAIWHGLYPGYMIFFV


 


QSALMINGSKVIYRWQQAVKQFRPPHYPVFTKLLHTP






(c) The partial nucleotide sequence of LPCAT from Capsicum annuum







(SEQ ID NO: 12)


GGCACGAGAAACGGTTGGGTTACCAATATATGGCTGGCTTTACTGCCCG


 


GTGGAAGTATTATTTTATCTGGTCAATCTCTGAAGCTGCTATAATCATA


 


TCTGGACTGGGTTTCAGTGGTTGGACAGACTCTTCTCCGCCAAAACCAC


 


GTTGGGACCGTGCAAAAAATGTTGATGTATTGGGTGTTGAGTTAGCAAA


 


GAGCTCGGTTCAGTTGCCTGCTGTCTGGAACATTCAAGTCAGCACATGG


 


CTGCGGCATTATGTATATGAAAGGCTCATACAAAAGGGAAGGAAGCCTG


 


GTTTCTTCCAGTTACTGGCTACCCAAACTGTCAGTGCCGTATGGCATGG


 


ATTATATCCTGGGTATATCATATTCTTTGTACAGTCCGCTTTGATGATT


 


GCTGGATCAAGAGTCCTTTACAGATGGCAGCAAGCTGCTAAAGGTTCTA


 


TGTTTGAGAAGATACTGGTAGCAATGAATTTTGCATACACACTGCTGGT


 


TCTAAATTACTCCGCTGTTGGGTTCATGGTATTAAGCCTGCATGAAACT


 


CTTACTGCTTATGGAAGTGTATACTATGTTGGAACAATTATACCAATTG


 


CTCTCATCCTGCTCAGTAAAGTAATTAAGCCTCCAAGACCCTGCACATC


 


TAAAG






The partial amino acid sequence of LPCAT from Capsicum annuum







(SEQ ID NO: 13)


HEKRLGYQYMAGFTARWKYYFIWSISEAAIIISGLGFSGWTDSSPPKPRW


 


DRAKNVDVLGVELAKSSVQLPAVWNIQVSTWLRHYVYERLIQKGRKPGFF


 


QLLATQTVSAVWHGLYPGYIIFFVQSALMIAGSRVLYRWQQAAKGSMFEK


 


ILVAMNFAYTLLVLNYSAVGFMVLSLHETLTAYGSVYYVGTIIPIALILL


 


SKVIKPPRPCTSK






(d) The partial nucleotide sequence of LPCAT from castor bean







(SEQ ID NO: 14)


ATTCATTTATACTTGGTGCCCCACTATCCTTTATCCCGGTTCACTGATCC


 


TGTGTACCAAGAATGGGGCTTCTGGAAACGATTAACTTATCAGTATATGT


 


CAGGTTTAACAGCACGTTGGAAATACTACTTCATCTGGTCAATTTCCGAG


 


GCCTCCATTATTATCTCTGGATTGGGTTTCAGTGGTTGGACAGATACTTC


 


TCCACCAAAGCCACAGTGGGATCGCGCTAGAAACGTTGACATTCTAGGTG


 


TTGAGTTTGCAAAGAGTGCAGCTGAGTTGCCACTTGTGTGGAACATACAA


 


GTCAGCACATGGCTTCGCCACTATGTTTATGATCGACTTGTTCCAAAGGG


 


AAAGAAAGCTGGTTTCCTTCAGTTGTTGGCCACTCAGACTACCAGTGCTG


 


TTTGGCATGGATTATATCCTGGATACATTATATTCTTTGTCCAGTCAGCA


 


TTAATGATTGCAGGTTCGAAAGTCATATACAGATGGCAACAAGCTATACC


 


TTCAAATAAGGCTCTTGAAAAGAAGATACTAGTGTTTATGAACTTTGCTT


 


ACACAGTTTTGGTTCTAAATTACTCCTGTGTTGGTTTCATGGTTTTAAGC


 


TTGCATGAAACGATTGCAGCATATGGAAGTGTATATTTTATTGGCACCAT


 


AGTGCCCGTTGTATTTTTCCTCCTTGGCTTCATTATTAAACCAGCAAGGC


 


CTTCCAGGTCTAAACACGGAACGATGAGTGAGGTAGAAACTGTTTTTCTT


 


CTCCTT






The partial amino acid sequence of LPCAT from castor bean







(SEQ ID NO: 15)


IHLYLVPHYPLSRFTDPVYQEWGFWKRLTYQYMSGLTARWKYYFIWSISE


 


ASIIISGLGFSGWTDTSPPKPQWDRARNVDILGVEFAKSAAELPLVWNIQ


 


VSTWLRHYVYDRLVPKGKKAGFLQLLATQTTSAVWHGLYPGYIIFFVQSA


 


LMIAGSKVIYRWQQAIPSNKALEKKILVFMNFAYTVLVLNYSCVGFMVLS


 


LHETIAAYGSVYFIGTIVPVVFFLLGFIIKPARPSRSKHGTMSEVETVFL


 


LL






(e) The partial nucleotide sequence of LPCAT from grapevine







(SEQ ID NO: 16)


CTCGTCCAATCTCCACTTCCTCGTTCCCATGCTTCTTGGCTACGCGGCTA


 


TGCTTCTCTGTCGCCGTCGATGCGGTGTGATCACCTTTTTCTTGGGATTC


 


GGCTACCTCATTGGCTGCCATGTATACTACATGAGTGGGGATGCATGGAA


 


GGAAGGGGGTATTGATGCTACTGGAGCTCTAATGGTTTTAACATTGAAAG


 


TCATTTCATGTGCAATGAATTATAATGATGGATTGTTAAAAGAAGACGGT


 


TTGCGTGAGGCACAGAAGAAAAACCGATTGCTTAAGTTACCATCATTGAT


 


CGAGTACTTTGGTTATTGTCTCTGCTGTGGAAGTCACTTTGCTGGACCAG


 


TTTATGAAATAAAGGATTATCTTGAATGGACAGAAAGAAAAGGGATTTGG


 


GCCAAATCAGAGAAAGGGCCACCACCATCACCTTATGGGGCAACGATTCG


 


AGCTCTTATCCAAGCTGCCTTTTGCATGGGCTTGTATGTGTATCTAGTAC


 


CCCATTTTCCCTTGACCATATTTACTGATCCTGTATATCAAGAATGGGGC


 


TTCTGGAAACGGTTGGGATACCAATATATGTGTGGCTTTACAGCACGCTG


 


GAAATACTATTTCATCTGGTCAATCTCTGAGGCAGCTGTCATTATTTCTG


 


GCCTGGGATTCAGTGGGTGGACAGAATCTTCCCCACCAAAACCAAAATGG


 


GACCGTGCAAAGAATGTTGACATTTTAGGTGTTGAGTTGGCAAAGAGTGC


 


AGTAACACTGCCACTTGTTTGGAACATACAAGTCAGCACCTGGCTACGTT


 


ATTATGTTTATGAGAGGCTCATTCAAAATGGGAAGAAACCTGGTTTCTTC


 


CAGTTGCTGGCTACACAAACTGTCAGTGCTGTTTGGCATGGATTATATCC


 


TGGATACATCATATTCTTTGTTCAGTCTGCACTGATG






The partial amino acid sequence of LPCAT from grapevine







(SEQ ID NO: 17)


SSNLHFLVPMLLGYAAMLLCRRRCGVITFFLGFGYLIGCHVYYMSGDAWK


 


EGGIDATGALMVLTLKVISCAMNYNDGLLKEDGLREAQKKNRLLKLPSLI


 


EYFGYCLCCGSHFAGPVYEIKDYLEWTERKGIWAKSEKGPPPSPYGATIR


 


ALIQAAFCMGLYVYLVPHFPLTIFTDPVYQEWGFWKRLGYQYMCGFTARW


 


KYYFIWSISEAAVIISGLGFSGWTESSPPKPKWDRAKNVDILGVELAKSA


 


VTLPLVWNIQVSTWLRYYVYERLIQNGKKPGFFQLLATQTVSAVWHGLYP


 


GYIIFFVQSALM






(f) The partial nucleotide sequence of LPCAT from maize







(SEQ ID NO: 18)


CATTTCGTGTCTCATAAACTACAGTGATGGTATCTTGAAGGAAGAGGGTT


 


TACGCGATGCTCAGATTAAACACCGATTGACTAAGCTTCCTTCTCTAATT


 


GAATATTTTGGGTACTGTCTCTGTTGTGGGAGCCACTTTGCTGGACCGGT


 


ATATGAGATGAAAGATTATCTTGAATGGACTGAAAGGAAAGGAATATGGG


 


CTAGCCCAACTCCTTCGCCATTGTTACCTACTTTGCGTGCTCTAGTTCAG


 


GCTGGTATATGCATGGGGTTATATTTATACCTGTCACCTAAATTTCCACT


 


CTCACGGTTTAGTGAGCCCCTATATTATGAATGGGGTTTTTGGCACCGAC


 


TCTTCTATCAGTACATGTCAGGCTTTACCGCTCGTTGGAAATATTACTTT


 


ATATGGTCAATTTCAGAAGCCTCAATTATCATATCTGGTCTAGGCTTTAC


 


TGGTTGGTCGGAATCTTCTCCCCCAAAAGCCAAATGGGATCGTGCAAAAA


 


ATGTTGATGTATTAGGTGTTGAATTAGCTGGAAGTTCAGTTCAATTGCCC


 


CTTGTGTGGAATATTCAAGTGAGCACATGGCTACGATACTATGTCTATGA


 


GAGGTTAATTCAGAAAGGAAAGAAACCAGGTTTCCTTCAATTGTTGGGTA


 


CACAGACAGTCAGTGCCATCTGGCATGGACTATATCCTGGATATATCATA


 


TTCTTTTTTTCATCAGCATTGATGATNAATGGTTCACGAGTTATATACAG


 


ATGGCAGCAAGCAGCGAGCAGTTCATTCCTGAGCGGTATCCTGGCCCTTC


 


TAATTTTGCTATACATTGCTGGGGCTTACTACTCCTGCATCGGGGTCCAG


 


GTACTGAGCTTCAA






The partial amino acid sequence of LPCAT from maize







(SEQ ID NO: 19)


ISCLINYSDGILKEEGLRDAQIKHRLTKLPSLIEYFGYCLCCGSHFAGPV


 


YEMKDYLEWTERKGIWASPTPSPLLPTLRALVQAGICMGLYLYLSPKFPL


 


SRFSEPLYYEWGFWHRLFYQYMSGFTARWKYYFIWSISEASIIISGLGFT


 


GWSESSPPKAKWDRAKNVDVLGVELAGSSVQLPLVWNIQVSTWLRYYVYE


 


RLIQKGKKPGFLQLLGTQTVSAIWHGLYPGYIIFFFSSALMXNGSRVIYR


 


WQQAASSSFLSGILALLILLYIAGAYYSCIGVQVLSF






(g) The partial nucleotide sequence of LPCAT from peach







(SEQ ID NO: 20)


AAATATTATTTCATCTGGTCAATTTCAGAGGCTTCTATCATTCTTTCTGG


 


TTTGGGTTTCACTGGCTGGACAGAATCTTCACCACCAAAGCCGCGATGGG


 


ATCGTGCAAAAAATGTTGATATTCTAGGCGTTGAGTTTGCAAAGAGTTCA


 


GTTCAGTTACCACTTGTTTGGAACATACAAGTCAGCACCTGGCTACGTCA


 


TTATGTTTATGAAAGGCTTGTTAAACCTGGCAAGAAGGCTGGTTTCTTCC


 


AGTTGCTGACTACACAGACCGTCAGTGCGGTTTGGCATGGACTCTATCCT


 


GGGTACATCATATTCTTTGTTCAGTCAGCATTGATGATTGCTGGTTCAAG


 


AGTGATTTACAGATGGCAACAAGCTGTACCTCAAAACATGGATGCTGTTA


 


AGAACATACTGGTGTTCATAAACTTTGCTTACACTCTCTTGGTTCTGAAC


 


TACTCCTGCGTTGGTTTCATTGTATTAAGCCTTCGTGAAACACTTGCCTC


 


ATATGGGAGCGTGCATTTCATCGGAACCATTCTTCCGATAGCATTGATAC


 


TACTGAGTTACGTAATAAAACCTCCAAGGCCTGCAAGATCAAAGGCTCGG


 


AAGGAAGAGTGAGGTTGTCANCCGCAACAGCATTTTTAACG






The partial amino acid sequence of LPCAT from peach







(SEQ ID NO: 21)


KYYFIWSISEASIILSGLGFTGWTESSPPKPRWDRAKNVDILGVEFAKSS


 


VQLPLVWNIQVSTWLRHYVYERLVKPGKKAGFFQLLTTQTVSAVWHGLYP


 


GYIIFFVQSALMIAGSRVIYRWQQAVPQNMDAVKNILVFINFAYTLLVLN


 


YSCVGFIVLSLRETLASYGSVHFIGTILPIALILLSYVIKPPRPARSKAR


 


KEE






(h) The full-length or partial amino acid sequence of LPCAT from rice
Sequence 1 (accession number Os02g0676000 (SEQ ID NO:22))







MGLEMEGMAAAIGVSVPVLRFLLCFAATIPTGLMWRAVPGAAGRHLYAGL


 


TGAALSYLSFGATSNLLFVVPMAFGYLAMLLCRRLAGLVTFLGAFGFLIA


 


CHMYYMSGDAWKEGGIDATGALMVLTLKIISCAINYSDGMLKEEGLRDAQ


 


KKYRLAKLPSLIEYFGYCLCCGSHFAGPVYEMKDYLEYTERKGLWASPTP


 


SPLLPTLRALVQAGACMGLYLYLSPQFPLSRFSEPLYYEWGFWHRLFYQY


 


MSGFTARWKYYFIWSLSEAAIIISGLGFSGWSDSSPPKAKWDRAKNVDVL


 


GVELATSAVQLPLMWNIQVSTWLRYYVYERLVQKGKKPGFLQLLGTQTVS


 


AVWHGLYPGYIIFFVQSALMINGSKVIYRWQQAVSNPVFHAILVFVNFSY


 


TLMVLNYSCIGFQVLSFKETLASYQSVYYIGTIVPIVVVLLGYVIKPARP


 


VKPKARKAE






Sequence 2 (accession number EAY87053 (SEQ ID NO:23))







MYYMSGDAWKEGGIDATGALMVLTLKIISCAINYSDGMLKEEGLRDAQKK


 


YRLAKLPSLIEYFGYCLCCGSHFAGPVYEMKDYLEYTERKGLWASPTPSP


 


LLPTLRALVQAGACMGLYLYLSPQFPLSRFSEPLYYEWGFWHRLFYQYMS


 


GFTARWKYYFIWSLSEAAIIISGLGFSGWSDSSPPKAKWDRAKNVDVLGV


 


ELATSAVQLPLMWNIQVSTWLRYYVYERLVQKGKKPGFLQLLGTQTVSAV


 


WHGLYPGYIIFFVQSALMINGSKVIYRWQQAVSNPVFHAILVFVNFSYTL


 


MVLNYSCIGFQFVFTMLYTLRFLQVLSFKETLASYQSVYYIGTIVPIVVV


 


LLGYVIKPARPVKPKARKAE






The partial nucleotide sequence of LPCAT from snapdragon







(SEQ ID NO: 24)


GCATTAATTACAACGATGGATTACTTAAAAAGGAAGATCTACGTGAGCCA


 


CAAAAGAAAAACCGCTTGCTCAAGATGCCATCATTACTTGAGTACATTGG


 


TTACTGTTTGTGTTGTGGAAGTCACTTTGCTGGTCCTGTGTATGAAATGA


 


AAGATTATCTTGAATGGACTGAGAGGAAAGGGATCTGGCAACATACAACC


 


AAGGGACCGAAACCTTCTCCGTATTGGGCGACTCTCAGGGCTATTTTGCA


 


AGCTGCCATCTGTATGGGCTTGTATCTATATCTTGTACCACATTACCCAC


 


TTTCCAGATTCACGGAGCCAGAATACCAAGAGTATGGGTTCTGGAAACGG


 


TTAAGTTACCAGTACATGTCAGGCTTCACCGCTCGTTGGAAGTACTATTT


 


CATTTGGTCTATCTCAGAAGCTTCCATAATTATTTCTGGCCTGGGGTTCA


 


GTGGCTGGACAGATTCTGATCCACCCAAAGCACTGTGGGATCGTGCAAAA


 


AATGTTGATGTATTAGGTGTTGAGTTGGCAAAGAGTTCTGTGCAGTTACC


 


ACTTGTATGGAATATTCAAGTTAGCACCTGGCTTAAACACTATGTCTATG


 


AGAGGCTGGTTCAGAAAGGTAAGAAACCAGGCTTCTTCCAGTTGCTGGCT


 


ACCCAGACCGTGAGTGCAGTGTGGCATGGATTGTACCCTGGGTACATCAT


 


ATTCTTT






The partial amino acid sequence of LPCAT from snapdragon







(SEQ ID NO: 25)


INYNDGLLKKEDLREPQKKNRLLKMPSLLEYIGYCLCCGSHFAGPVYEMK


 


DYLEWTERKGIWQHTTKGPKPSPYWATLRAILQAAICMGLYLYLVPHYPL


 


SRFTEPEYQEYGFWKRLSYQYMSGFTARWKYYFIWSISEASIIISGLGFS


 


GWTDSDPPKALWDRAKNVDVLGVELAKSSVQLPLVWNIQVSTWLKHYVYE


 


RLVQKGKKPGFFQLLATQTVSAVWHGLYPGYIIFF






(j) The partial nucleotide sequence of LPCAT from sorghum







(SEQ ID NO: 26)


GCACGAGGCTCTCACGGTTTAGTGAGCCCTTATATTATGAATGGGGTTTC


 


TGGCACCGACTCTTCTATCAGTACATGTCAGGCTTCACTGCTCGTTGGAA


 


ATATTACTTTATATGGTCAATTTCAGAAGCCTCAATTATCATATCTGGTC


 


TGGGCTTTACTGGTTGGTCAGAATCTTCTCCCCCGAAAGCCAAATGGGAT


 


CGTGCGAAAAATGTTGATGTATTAGGTGTTGAATTAGCTGGAAGTGCAGT


 


TCAAATTCCCCTTGTGTGGAATATTCAAGTGAGCACATGGTTACGATACT


 


ATGTCTATGAGAGGCTAATTCAGAAAGGAAAGAAACCAGGTTTCCTTCAG


 


TTGTTGGGTACACAGACAGTCAGCGCCATCTGGCATGGACTGTATCCTGG


 


ATATATCATATTCTTTGTTCAGTCAGCATTGATGATAAATGGTTCACGAG


 


TTATATACAGATGGCAGCAAGCAGTGAGCAGTTCATTCCTCCGCGGTATC


 


CTGGCTTTTCTAAATTTTGCTTATACATTGCTGGTGCTTAACTACTCCTG


 


CATCGGGTTCCTGGTACTGAGCTTCAAAGAAACCTTGGCGTCCTACCAGA


 


GCGTATATTATGTTGGCACAATTGTTCCCATTGTGTTTCTCCTGCTGGGC


 


AAT






The partial amino acid sequence of LPCAT from sorghum







(SEQ ID NO: 27)


TRLSRFSEPLYYEWGFWHRLFYQYMSGFTARWKYYFIWSISEASIIISGL


 


GFTGWSESSPPKAKWDRAKNVDVLGVELAGSAVQIPLVWNIQVSTWLRYY


 


VYERLIQKGKKPGFLQLLGTQTVSAIWHGLYPGYIIFFVQSALMINGSRV


 


IYRWQQAVSSSFLRGILAFLNFAYTLLVLNYSCIGFLVLSFKETLASYQS


 


VYYVGTIVPIVFLLLGN






(k) The partial nucleotide sequence of LPCAT from sunflower







(SEQ ID NO: 28)


GAAAACCGCATACTTAAGTTGCCATCTTTAATCGAGTATGTGGGATATTG


 


CTTATGCTGCGGAAGTCACTTTGCTGGTCCGGTTTACGAAATCAAAGATT


 


ATTTGGATTGGACCGAAAGAAAGGGGATTTGGACAAAGTCCGAGAAAGGC


 


ACACCATCACCATTTTTGCCAACACTACGAGCGATTCTCCAAGCGGGTTT


 


CTGTATGGGTTTGTATTTATATCTATCGCCTTCGTATCCGCTTTCAAGAT


 


TCAGTGAGCCGATATATCAAGAATGGGGATTTGTGAAACGTCTGACCGTC


 


CAATACATGTCGGGCTTCACCGCGCGTTGGAAATACTATTTCATTTGGTC


 


TATCTCAGAAGCTTCTATCATTATTTCGGGCTTCGGTTTCAGTGGCTGGA


 


CTGATTCTTCTCCACCAAAAGCCCGATGGGACCGTGCGAAAAACGTTGAC


 


GTTTTGGGTGTTGAGTTTGCAAAGAGTTCAGTTGAGTTACCACTCGTGTG


 


GAATATCCAAGTCAGCACATGGCTTCGTCACTATGTTTATGACAGACTTG


 


TTCAAAAGGGAAAGAAGCCTGGCTTTTTCCAATTGTTAGCAACACAGACT


 


GTTAGCGCTGTCTGGCATGGATTATATCCTGGGTACTTGATATTCTTTGT


 


TCAATCTGCTTTGATGATTTCCGGGTCAAGAGCCATTTACAGATGGCAGC


 


AGGCGGTTCCGCCAACCGTTAAGAAGTTTTTGATGCTCATGAACTTTGCT


 


TACACGCTTCTTGTTCTTAACTACTCCTGCATAGGTTTTATGGTATTAAG


 


CCTACACGAAACACTGGCTGCATACGGAAGTGTATACTACGTTGGAAACA


 


TCATTCCAGTGGCGT






The partial amino acid sequence of LPCAT from sunflower







(SEQ ID NO: 29)


ENRILKLPSLIEYVGYCLCCGSHFAGPVYEIKDYLDWTERKGIWTKSEKG


 


TPSPFLPTLRAILQAGFCMGLYLYLSPSYPLSRFSEPIYQEWGFVKRLTV


 


QYMSGFTARWKYYFIWSISEASIIISGFGFSGWTDSSPPKARWDRAKNVD


 


VLGVEFAKSSVELPLVWNIQVSTWLRHYVYDRLVQKGKKPGFFQLLATQT


 


VSAVWHGLYPGYLIFFVQSALMISGSRAIYRWQQAVPPTVKKFLMLMNFA


 


YTLLVLNYSCIGFMVLSLHETLAAYGSVYYVGNIIPVA






(1) The partial nucleotide sequence of LPCAT from tomato







(SEQ ID NO: 30)


GGTATGGGGTTGTATCTCTATCTGGTGCCTCAGTTCCCACTTTCCAGGTT


 


CACTGAGTCAGTATACCACGAATGGGGTTTCTTCAAACGACTGGGTTACC


 


AATATATGGCTGGCTTTACTGCCCGGTGGAAATATTATTTTATTTGGTCA


 


ATCTCTGAAGCTTCTATAATCATATCTGGACTGGGTTTCAGTGGTTGGAC


 


AAACTCTTCTCCGCCAAAACCACGTTGGGACCGAGCAAAAAATGTTGATG


 


TATTGGGTGTTGAGTTAGCAAAGAGCTCGGTTCAGTTACCACTAGTATGG


 


AACATTCAAGTCAGCACATGGCTGCGGCATTATGTGTATGAAAGGCTCGT


 


ACAGAAGGGAAGGAAGCCTGGTTTCTTCCAGTTGCTGGCTACCCAAACTG


 


TCAGTGCCGTTTGGCATGGATTATATCCTGGATACATCATATTCTTTGTT


 


CAGTCCGCTTTGATGATTGCTGGATCAAGAGTCATTTACAGATGGCAGCA


 


AGCTACAAAAGGTACTATGTTTGAGAAGATACTGATAGCAATGAATTTTG


 


CATACACACTGCTGGTTCTAAACTACTCCGCTGTTGGATTCATGGTATTA


 


AGTCTGCATGAAACTCTTACTGCTTATGGAAGTGTATACTATATTGGAAC


 


AATTGTACCAATTCTTCTCATCCTGCTTAGTAAAGTGATTAAGCCTCCAA


 


GACCTGCGACGTCTAAAGCTAGGAAAGCAGAGTAAATCCAAGTCAGTT






The partial amino acid sequence of LPCAT from tomato







(SEQ ID NO: 31)


GMGLYLYLVPQFPLSRFTESVYHEWGFFKRLGYQYMAGFTARWKYYFIWS


 


ISEASIIISGLGFSGWTNSSPPKPRWDRAKNVDVLGVELAKSSVQLPLVW


 


NIQVSTWLRHYVYERLVQKGRKPGFFQLLATQTVSAVWHGLYPGYIIFFV


 


QSALMIAGSRVIYRWQQATKGTMFEKILIAMNFAYTLLVLNYSAVGFMVL


 


SLHETLTAYGSVYYIGTIVPILLILLSKVIKPPRPATSKARKAE






(m) The partial nucleotide sequence of LPCAT from Vaccinium corymbosum







(SEQ ID NO: 32)


GGGGTTGGGTTACCAGTACATGGCTGGCTTTACAGCACGGTGGAAGTATT


 


ATTTCATTTGGTCAATCTCAGAAGCTTCCATCATCATTTCTGGCCTGGGG


 


TTCAGTGGTTGGACAGATTCTTCTCCACCAAAACCAAAATGGGACCGTGC


 


AAAGAATGTAGATATTTTGCGGGTTGAGTTTGCAAAGACTGCAGCTCAGA


 


TTCCACTTGCATGGAACATTCAAGTCAGCACCTGGCTACGCCATTATGTT


 


TATGAGAGGCTCGTGCAGAAGGGAAAGAAACCTGGTTTCTTTCAGTTGTT


 


GGCTACCCAGACTGTCAGTGCTGTTTGGCATGGTTTATATCCTGGATACA


 


TCATATTCTTTGTGCAGTCAGCATTGATGATTGCTGGTTCAAGAGTTATT


 


TATAGATGGCAGCAAGCTGTTCCTCCTAAAATGGATCTGGTGAAGAAAGT


 


ATTCGTACTTTTAAACTTTGCTTACACAGTTCTGGTGTTGAACTACTCCT


 


CTGTCGGTTTCATGGTACTAAGCCTACATGAAACAATTGTTGCATACGGG


 


AGCGTGTATTCGTTGGAACCATTGTTCCCATACTTGTAATCCTCCTTGGT


 


TACGTAATT






The partial amino acid sequence of LPCAT from Vaccinium corymbosum







(SEQ ID NO: 33)


GLGYQYMAGFTARWKYYFIWSISEASIIISGLGFSGWTDSSPPKPKWDRA


 


KNVDILRVEFAKTAAQIPLAWNIQVSTWLRHYVYERLVQKGKKPGFFQLL


 


ATQTVSAVWHGLYPGYIIFFVQSALMIAGSRVIYRWQQAVPPKMDLVKKV


 


FVLLNFAYTVLVLNYSSVGFMVLSLHETIVAYGSVYSLEPLFPYL






(n) The partial nucleotide sequence of LPCAT from wheat







(SEQ ID NO: 34)


CACTTTGCTGGACCAGTATATGAGATGAAAGATTATCTTGAATGGACTGA


 


AAGGAAAGGAATATGGGCCGGCTCAACTCCTTCACCATTATTACCTACTC


 


TGCGTGCTCTAGTTCAGGCTGGAATATGCATGGGGTTATATTTGTATCTG


 


TCACCTATGTTTCCCCATTCATAATATAGAGGTTCACTAAATCGTGAAAG


 


GGGTTTCTGGCACCGGCTCTTCTTTCAATACATGTCAGGATTTACTGCTC


 


GATGGAAATACTACTTTATATGGTCAGTCTCAGAAGCTGCAATTATTATA


 


TCTGGCCTGGGTTTCACTGGTTGGTCTGATTCTTCTCCCCCAAAAGCCAA


 


ATGGGACCGTGCTATAAATGTTGATATTCTGGGCGTCGAGCTAGCTGGAA


 


GTGCAGCTCAATTGCCACTTAAGTGGAATATTCAAGTGAGCACATGGCTA


 


AGATACTATGTGTATGAGAGGTTAATTCAGAAAGGGAAGAAGCCTGGTTT


 


CCTTCAGTTGTTGGGTACACAGACAGTCAGTGCTATCTGGCATGGACTGT


 


ATCCAGGATATATGTTTTTCTTTGTTCAGTCAGCGTTGATGATAAATGGT


 


TCAAAAGTTATATACAGATGGCAACAAGCTGTGAGCAATCCAGGCCTCCG


 


CACTATCCTGTCTTTACTAAATTGTGCATACACCATGATGGTGCTTAACT


 


ACTCATGCATTGGCTTCCAGGTACTGAGCTTCCAGGAGACCTTAGCATCC


 


TACAAGAGCGTGTATTATGTCGGCACAATCGTTCCTATTCTATGTGTCTT


 


GCTGGGCTATGTCGTCAAGCCCACGAGACCTGTGAAGCCGA






The partial amino acid sequence of LPCAT from wheat







(SEQ ID NO: 35)


HFAGPVYEMKDYLEWTERKGIWAGSTPSPLLPTLRALVQAGICMGLYLYL


 


SPMFPHS*YRGSLNRERGFWHRLFFQYMSGFTARWKYYFIWSVSEAAIII


 


SGLGFTGWSDSSPPKAKWDRAINVDILGVELAGSAAQLPLKWNIQVSTWL


 


RYYVYERLIQKGKKPGFLQLLGTQTVSAIWHGLYPGYMFFFVQSALMING


 


SKVIYRWQQAVSNPGLRTILSLLNCAYTMMVLNYSCIGFQVLSFQETLAS


 


YKSVYYVGTIVPILCVLLGYVVKPTRPVKP






(o) The amino acid sequences of LPCAT from A. thaliana 
Sequence (accession number At1g12640 (SEQ ID NO:36))







MDMSSMAGSIGVSVAVLRFLLCFVATIPVSFACRIVPSRLGKHLYAAASG


 


AFLSYLSFGFSSNLHFLVPMTIGYASMAIYRPKCGIITFFLGFAYLIGCH


 


VFYMSGDAWKEGGIDSTGALMVLTLKVISCSMNYNDGMLKEEGLREAQKK


 


NRLIQMPSLIEYFGYCLCCGSHFAGPVYEMKDYLEWTEGKGIWDTTEKRK


 


KPSPYGATIRAILQAAICMALYLYLVPQYPLTRFTEPVYQEWGFLRKFSY


 


QYMAGFTARWKYYFIWSISEASIIISGLGFSGWTDDASPKPKWDRAKNVD


 


ILGVELAKSAVQIPLVWNIQVSTWLRHYVYERLVQNGKKAGFFQLLATQT


 


VSAVWHGLYPGYMMFFVQSALMIAGSRVIYRWQQAISPKMAMLRNIMVFI


 


NFLYTVLVLNYSAVGFMVLSLHETLTAYGSVYYIGTIIPVGLILLSYVVP


 


AKPSRPKPRKEE






Sequence (accession number At1g63050 (SEQ ID NO:37))







MELLDMNSMAASIGVSVAVLRFLLCFVATIPISFLWRFIPSRLGKHIYSA


 


ASGAFLSYLSFGFSSNLHFLVPMTIGYASMAIYRPLSGFITFFLGFAYLI


 


GCHVFYMSGDAWKEGGIDSTGALMVLTLKVISCSINYNDGMLKEEGLREA


 


QKKNRLIQMPSLIEYFGYCLCCGSHFAGPVFEMKDYLEWTEEKGIWAVSE


 


KGKRPSPYGAMIRAVFQAAICMALYLYLVPQFPLTRFTEPVYQEWGFLKR


 


FGYQYMAGFTARWKYYFIWSISEASIIISGLGFSGWTDETQTKAKWDRAK


 


NVDILGVELAKSAVQIPLFWNIQVSTWLRHYVYERIVKPGKKAGFFQLLA


 


TQTVSAVWHGLYPGYIIFFVQSALMIDGSKAIYRWQQAIPPKMAMLRNVL


 


VLINFLYTVVVLNYSSVGFMVLSLHETLVAFKSVYYIGTVIPIAVLLLSY


 


LVPVKPVRPKTRKEE






The amino acid sequences of LCPAT from fruit fly, human, mouse, S. pombe, and Aspergillus oryzae. 
The amino acid sequences of LCPAT from fruit fly
Sequence 1 (accession number AAR99097 (SEQ ID NO:38))







MLEPPKFIENDCYNGSRTFTWLADMVGLSVDLVNFLICQISALFLASLFR


 


SMLHPSKVSSKLRHTFALSIGLAFGYFCFGQQAIHIAGLPAICYIVIRTQ


 


DPRIVQRAVLLVAMSYLLCVHLMRQLYDYGSYALDITGPLMIITQKVTSL


 


AFSIHDGFVRGDEELTKAQQYHAIRKMPSALEYFSYVWHFQSILAGPLVF


 


YKDYIEFVEGYNLLSTPPGNGNLDSSKREVVLEPSPTKAVIRKVVGSLVC


 


AFIFMKFVKIYPVKDMKEDDFMNNTSMVYKYWYAMMATTCIRFKYYHAWL


 


LADAICNNSGLGFTGYDKDGNSKWDLISNINVLSFEFSTNMRDAINNWNC


 


GTNRWLRTLVYERVPQQYGTLLTFALSAVWHGFYPGYYLTFATGAVVVTA


 


ARTGRRLFRHRFQSTQVTRMFYDILTCLITRVVLGYATFPFVLLEFMGSI


 


KLYLRFYLCLHIISLVTIFILPKFIRGERRLRTSNGNGNVRLSGSGNTKD


 


AVTTSVESTAALTAGNDLNEDKEEDKHAQCKVHTPTQQQPAAGPHKTTVE


 


QPTEQPNNVNLRSRPQQQQPHLEKKAMPPTCARDAVSVPHDQCEMDQLSS


 


KLKEKIEAETKNIEEFIDKTVTETVSGIVEFKNDLMRDIEFPKLKLPGSN


 


GAISLDSSNGGGLRKRNISSVHDNGTDPGHATADLHPPLEENGAAFLKKE


 


IEVINAVVQQAVPAVLSNGHAK






Sequence 2 (accession number AAO41223 (SEQ ID NO:39))







MAEFEEDLPHNGLMDGIASGVGVPVEALRLLLTILAGYPVAALYQKFISV


 


IADKTVHHMFFAGCGAGLCYFNYGLDTYHSLIAILTTYFLVLLLRKKTQI


 


FLAINFVFHMSYLLLGYFYTSSNDYDILWTMPHCILVLRMIGYGFDITDG


 


LKEESELSKDQKETALKKPPSLLELLAFSYFPSGFLVGPQFPFRRYKAFV


 


DGEFRQHEGNVEAGVRRFGAGAFYLIVCQVGLRYLPDSYFLTPEFAQVSF


 


VKRIYLLGFWAKFSLYKYISCWLLTEGALICIGLTYKGEDKNGQPDWSGC


 


SNVKLKLLETGNTMEHYVQSFNVNTNQWVGQYIYKRLKFLNNRTISYGAA


 


LGFLAVWHGYHSGYYMTFLMEYMVVSTEKQITRFYTKVVLPQWGHILNNS


 


DIYKLLYFITLKSYNVVYMGWCLTAFVFLKYERWIVVYGAVSYYGFTFLV


 


LWAAFYHTFNHFFRSSSRKLAGEDQKLQDSNTDKLVEEKKPEDKKSE






(2) The amino acid sequences of LCPAT from human
Sequence 1 (accession number EAX01013 (SEQ ID NO:40))







MKCCFHHIIPRVNFVVCQLFALLAAIWFRTYLHSSKTSSFIRHVVATLLG


 


LYLALFCFGWYALHFLVQSGISYCIMIIIGVENMHNYCFVFALGYLTVCQ


 


VTRVYIFDYGQYSADFSGPMMIITQKITSLACEIHDGMFRKDEELTSSQR


 


DLAVRRMPSLLEYLSYNCNFMGILAGPLCSYKDYITFIEGRSYHITQSGE


 


NGKEETQYERTEPSPNTAVVQKLLVCGLSLLFHLTICTTLPVEYNIDEHF


 


QATASWPTKITYLYISLLAARPKYYFAWTLADAINNAAGFGFRGYDENGA


 


ARWDLISNLRIQQIEMSTSFKMFLDNWNIQTALWLKRVCYERTSFSPTIQ


 


TFILSAIWHGVYPGYYLTFLTGVLMTLAARAMRNNFRHYFIEPSQLKLFY


 


DVITWIVTQVAISYTVVPFVLLSIKPSLTFYSSWYYCLHILGILVLLLLP


 


VKKTQRRKNTHENIQLSQSKKFDEGENSLGQNSFSTTNNVCNQNQEIASR


 


HSSLKQ






Sequence 2 (accession number Q6ZWT7 (SEQ ID NO:41))







MATTSTTGSTLLQPLSNAVQLPIDQVNFVVCQLFALLAAIWFRTYLHSSK


 


TSSFIRHVVATLLGLYLALFCFGWYALHFLVQSGISYCIMIIIGVENMHN


 


YCFVFALGYLTVCQVTRVYIFDYGQYSADFSGPMMIITQKITSLACEIHD


 


GMFRKDEELTSSQRDLAVRRMPSLLEYLSYNCNFMGILAGPLCSYKDYIT


 


FIEGRSYHITQSGENGKEETQYERTEPSPNTAVVQKLLVCGLSLLFHLTI


 


CTTLPVEYNIDEHFQATASWPTKIIYLYISLLAARPKYYFAWTLADAINN


 


AAGFGFRGYDENGAARWDLISNLRIQQIEMSTSFKMFLDNWNIQTALWLK


 


RVCYERTSFSPTIQTFILSAIWHGVYPGYYLTFLTGVLMTLAARAMRNNF


 


RHYFIEPSQLKLFYDVITWIVTQVAISYTVVPFVLLSIKPSLTFYSSWYY


 


CLHILGILVLLLLPVKKTQRRKNTHENIQLSQSRKFDEGENSLGQNSFST


 


TNNVCNQNQEIASRHSSLKQ






Sequence 3 (accession number Q6P1A2 (SEQ ID NO:85))







MASSAEGDEGTVVALAGVLQSGFQELSLNKLATSLGASEQALRLIISIFL


 


GYPFALFYRHYLFYKETYLIHLFHTFTGLSIAYFNFGNQLYHSLLCIVLQ


 


FLILRLMGRTITAVLTTFCFQMAYLLAGYYYTATGNYDIKWTMPHCVLTL


 


KLIGLAVDYFDGGKDQNSLSSEQQKYAIRGVPSLLEVAGFSYFYGAFLVG


 


PQFSMNHYMKLVQGELIDIPGKIPNSIIPALKRLSLGLFYLVGYTLLSPH


 


ITEDYLLTEDYDNHPFWFRCMYMLIWGKFVLYKYVTCWLVTEGVCILTGL


 


GFNGFEEKGKAKWDACANMKVWLFETNPRFTGTIASFNINTNAWVARYIF


 


KRLKFLGNKELSQGLSLLFLALWHGLHSGYLVCFQMEFLIVIVERQAARL


 


IQESPTLSKLAAITVLQPFYYLVQQTIHWLFMGYSMTAFCLFTWDKWLKV


 


YKSIYFLGHIFFLSLLFILPYIHKAMVPRKEKLKKME






Sequence 4 (accession number Q6ZNC8 (SEQ ID NO:86))








    MAAEPQPSSLSYRTTGSTYLHPLSELLGIPLDQVNFVVCQLVALFAAFWFRIYLRP



 


GTTSSDVRHAVATIFGIYFVIFCFGWYSVHLFVLVLMCYAIMVTASVSNIHRYSFFVAMG


 


YLTICHISRIYIFHYGILTTDFSGPLMIVTQKITTLAFQVHDGLGRRAEDLSAEQHRLAIKV


 


KPSFLEYLSYLLNFMSVIAGPCNNFKDYIAFIEGKHIHMKLLEVNWKRKGFHSLPEPSPT


 


GAVIHKLGITLVSLLLFLTLTKTFPVTCLVDDWFVHKASFPARLCYLYVVMQASKPKYY


 


FAWTLADAVNNAAGFGFSGVDKNGNFCWDLLSNLNIWKIETATSFKMYLENWNIQTA


 


TWLKCVCYQRVPWYPTVLTFILSALWHGVYPGYYFTFLTGILVTLAARAVRNNYRHYF


 


LSSRALKAVYDAGTWAVTQLAVSYTVAPFVMLAVEPTISLYKSMYFYLHIISLLIILFLP


 


MKPQAHTQRRPQTLNSINKRKTD






Sequence 5 (accession number XP—001129292 (SEQ ID NO:87))








    MVMMMMMKVLLLLMKQRGAGLPAPAGVEPRPSSHHPKARVRLQGDESVRPRG



 


CSQLWAFTRHSPRQRGFSARSLFWFVVLPAPTFVPNFPWRWLGGVPHIVPPAATPGPFV


 


VCRLSQRGVGGRDIPGRRNRGVRGKDALPCSHPRSAPHDAGQPFSGDARHPRAEREVG


 


RALLPATAPGEGGRMGVRVCMRSLPFAAAALGSGGRVPEQPPVRMDRVVERVRKAAL


 


WGAWRGAACPARASERPPERLMHGSGDGLLGFSFVRASLTVFGEEAGPSFLLAVLCAV


 


VWGGRGEDVVSDVQACPAEQGFLLAEPSVFGVNFVVCQLFALLAAIWFRTYLHSSKTS


 


SFIRHVVATLLGLYLALFCFGWYALHFLVQSGISYCIMIIIGVENMHNYCFVFALGYLTV


 


CQVTRVYIFDYGQYSADFSGPMMIITQKITSLACEIHDGMFRKDEELTSSQRDLAVRRMP


 


SLLEYLSYNCNFMGILAGPLCSYKDYITFIEGRSYHITQSGENGKEETQYERTEPSPNTAV


 


VQKLLVCGLSLLFHLTICTTLPVEYNIDEHFQATASWPTKIIYLYISLLAARPKYYFAWTL


 


ADAINNAAGFGFRGYDENGAARWDLISNLRIQQIEMSTSFKMFLDNWNIQTALWLKRV


 


CYERTSFSPTIQTFILSAIWHGVYPGYYLTFLTGVLMTLAARAMRNNFRHYFIEPSQLKLF


 


YDVITWIVTQVAISYTVVPFVLLSIKPSLTFYSSWYYCLHILGILVLLLLPVKKTQRRKNT


 


HENIQLSQSKKFDEGENSLGQNSFSTTNNVCNQNQEIASRHSSLKQ






Sequence 6 (accession number XP—001131044 (SEQ ID NO:88))








    MVNFVVCQLVALFAAFWFRIYLRPGTTSSDVRHAVATIFGIYFVIFCFGWYSVHL



 


FVLVLMCYAIMVTASVSNIHRYSFFVAMGYLTICHISRIYIFHYGILTTDFSGPLMIVTQKI


 


TTLAFQVHDGLGRRAEDLSAEQHRLAIKVKPSFLEYLSYLLNFMSVIAGPCNNFKDYIAF


 


IEGKHIHMKLLEVNWKRKGFHSLPEPSPTGAVIHKLGITLVSLLLFLTLTKTFPVTCLVDD


 


WFVHKASFPARLCYLYVVMQASKPKYYFAWTLADAVNNAAGFGFSGVDKNGNFCWD


 


LLSNLNIWKIETATSFKMYLENWNIQTATWLKCVCYQRVPWYPTVLTFILSALWHGVY


 


PGYYFTFLTGILVTLAARAVRNNYRHYFLSSRALKAVYDAGTWAVTQLAVSYTVAPFV


 


MLAVEPTISLYKSMYFYLHIISLLIILFLPMKPQAHTQRRPQTLNSINKRKTD






(3) The amino acid sequences of LCPAT from mouse
Sequence 1 (accession number AAH24653 (SEQ ID NO:42))








    MAARPPASLSYRTTGSTCLHPLSQLLGIPLDQVNFVACQLFALSAAFWFRIYLHP



 


GKASPEVRHTLATILGIYFVVFCFGWYAVHLFVLVLMCYGVMVSASVSNIHRYSFFVA


 


MGYLTICHISRIYIFHYGILTTDFSGPLMIVTQKITTLAFQVHDGLGRKAEDLSAEQHRLA


 


VKAKPSLLEYLSYHLNFMSVIAGPCNNFKDYVAFIEGRHIHMKLLEVNWTQRGFQSLPE


 


PSPTGAVIQKLCVTLMSLLLFLTLSKSFPVTFLIDDWFVHKANFLSRLWYLYVVMQAAK


 


PKYYFAWTLADAVHNAAGFGFNGMDTDGKSRWDLLSNLNIWKIETATSFKMYLENWN


 


IQTSTWLKCVCYERVSWYPTVLTFLLSALWHGVYPGYYFTFLTGVPVTLAARAVRNNY


 


RHHFLSSKARKIAYDVVTWAVTQLAVSYTAAPFVMLAVEPTISLYKSVFFFLHIICLLIIL


 


FLPIKPHQPQRQSRSPNSVKKKAD






Sequence 2 (accession number AAH25429 (SEQ ID NO:43))








    MATTSTTGSTLLQPLSNAVQLPIDQVNFVVCQLFALLAAVWFRTYLHSSKTSSFIR



 


HVVATLLGLYLAFFCFGWYALHFLVQSGISYCIMIIAGVESMQQCCFVFALGYLSVCQIT


 


RVYIFDYGQYSADFSGPMMIITQKITSLAYEIHDGMFRKDEELTPSQRGLAVRRMPSLLE


 


YVSYTCNFMGILAGPLCSYKDYIAFIEGRASHVAQPSENGKDEQHGKADPSPNAAVTEK


 


LLVCGLSLLFHLTISNMLPVEYNIDEHFQATASWPTKATYLYVSLLAARPKYYFAWTLA


 


DAINNAAGFGFRGYDKNGVARWDLISNLRIQQIEMSTSFKMFLDNWNIQTALWLKRVC


 


YERATFSPTIQTFFLSAIWHGVYPGYYLTFLTGVLMTLAARAVRNNFRHYFLEPPQLKLF


 


YDLITWVATQITISYTVVPFVLLSIKPSFTFYSSWYYCLHVCSILVLLLLPVKKSQRRTSTQ


 


ENVHLSQAKKFDERDNPLGQNSFSTMNNVCNQNRDTGSRHSSLTQ






(4) The amino acid sequences of LCPAT from S. pombe 
Sequence (accession number CAA16861 (SEQ ID NO:44))








     MAYLIDIPFEYFSSFLGVHPDQLKLLFCFLSAYPFAGILKRLPSAPWIRNLFSISIGL



 


FYLIGVHHLYDGVLVLLFDALFTYFVAAFYRSSRMPWIIFIVILGHTFSSHVIRYIYPSENT


 


DITASQMVLCMKLTAFAWSVYDGRLPSSELSSYQKDRALRKIPNILYFLGYVFFFPSLLV


 


GPAFDYVDYERFITLSMFKPLADPYEKQITPHSLEPALGRCWRGLLWLILFITGSSIYPLK


 


FLLTPKFASSPILLKYGYVCITAFVARMKYYGAWELSDGACILSGIGYNGLDSSKHPRW


 


DRVKNIDPIKFEFADNIKCALEAWNMNTNKWLRNYVYLRVAKKGKRPGFKSTLSTFTV


 


SAMWHGVSAGYYLTFVSAAFIQTVAKYTRRHVRPFFLKPDMETPGPFKRVYDVIGMVA


 


TNLSLSYLIISFLLLNLKESIHVWKELYFIVHIYILIALAVFNSPIRSKLDNKIRSRVNSYKL


 


KSYEQSMKSTSDTDMLNMSVPKREDFENDE






(5) The amino acid sequences of LCPAT from Aspergillus oryzae 
Sequence (accession number BAE61812 (SEQ ID NO:45))








    MLPYVDLLKLIASFLLSYPLAALLKRIPDAQPWKKNAFIIAVSLFYLVGLFDLWD



 


GLRTLAYSAAGIYAIAYYIDGSLMPWIGFIFLMGHMSISHIYRQIIDDAHVTDITGAQMVL


 


VMKLSSFCWNVHDGRLSQEQLSDPQKYAAIKDFPGILDYLGYVLFFPSLFAGPSFEYVD


 


YRRWIDTTLFDVPPGTDPSKVPPTRKKRKIPRSGTPAAKKALAGLGWILAFLQLGSLYN


 


QELVLDETFMQYSFVQRVWILHMLGFTARLKYYGVWYLTEGACVLSGMGYNGFDPKS


 


GKVFWNRLENVDPWSLETAQNSHGYLGSWNKNTNHWLRNYVYLRVTPKGKKPGFRA


 


SLATFVTSAFWHGFYPGYYLTFVLGSFIQTVAKNFRRHVRPFFLTPDGSRPTAYKKYYDI


 


ASYVVTQLTLSFAVMPFIFLSFGDSIKVWHSVYFYGIVGNIVSLAFFVSPARGLLLKKLK


 


ARNKPHVPRAVSSENIRQPTLGLPNDAIQEFDDAVQEIRAEIESRQRRGSLAHMPIGDEL


 


KAAVEDKIGRGH






Alignment of the LPCAT sequences from different species that reveals four conserved motifs unique for this novel type of LPCAT enzymes (FIG. 2). They are not present in the previously identified glycerol-3-phosphate acyltransferases, lyso-phosphatidic acid acyltransferases, and known LPCAT enzymes. The sequences of these motifs are as follows. The letter “φ” represents a certain amino acid.
Motif 1: M V(I) L(I) φ φ L(V,I) φ φ φ φ φ φ φ φ D G (or Met Xaa Xaa Xaa Xaa Lys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Asp Gly (SEQ ID NO:46), wherein the Xaa at position 2 can be Val or Ile, the Xaa at position 3 can be Leu, Val or Ile, the Xaa at position 7 can be Leu, Val, Met, or Ile, while the other Xaa's in the motif may be any amino acid.
Motif 2: R φ K Y Y φ φ W φ φ φ E(D) A(G) φ φ φ φ φ Gφ G F(Y) φ G (or Arg Xaa Lys Tyr Tyr Xaa Xaa Trp Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Gly Xaa Gly Xaa Xaa Gly (SEQ ID NO:47), wherein the Xaa at position 12 is Glu or Asp, wherein the Xaa at position 13 is Ala or Gly, wherein the Xaa at position 22 is Phe or Tyr, while the other Xaa's in the motif may be any amino acid.
Motif 3: E φ φ φ φ φ φ φ φ φ φ φ W N φ φ T(V) φ φ W (or Glu Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Trp Asn Xaa Xaa Xaa Xaa Xaa Trp (SEQ ID NO:48) wherein the Xaa at position 17 is Thr or Val, while the other Xaa's in the motif may be any amino acid.
Motif 4: S A φ W H G φ φ P G Y φ φ T(F) F (or Ser Ala Xaa Trp His Gly Xaa Xaa Pro Gly Tyr Xaa Xaa Xaa Phe (SEQ ID NO:49) wherein Xaa at position 14 is Thr or Phe, while the other Xaa's in the motif may be any amino acid.
FIG. 3 depicts another alignment of LPCAT sequences from different plant species that revealed the following motifs:







Motif 5 (SEQ ID NO: 81):


E A φ φ I I(L) S G φ G F S(T) G W;


 


Motif 6 (SEQ ID NO: 82):


W D R A φ N V D;


 


Motif 7 (SEQ ID NO: 83):


W N I Q V S T W L φ φ Y V Y;


and


 


Motif 8 (SEQ ID NO: 84):


G F φ Q L L φ T Q T φ S A φ W H G L Y P G Y.






Example 7
Analysis of LPCAT from the Alga Thalassiosira pseudonana 
Materials and Methods
Isolation of the LPCAT cDNA from T. pseudonana: PCR primers were designed for nucleotide sequence of the putative TpLPCAT obtained by a BLAST search of the sequenced T. pseudonana genome using the yeast LPCAT sequence. Plasmid from a cDNA library of T. pseudonana was used as template. A 50 μl PCR reaction contained 50 ng of plasmid DNA, 20 pM of each primer: 5′-GGTATGCTCATCTGCTACCCCCTC-3′ (SEQ ID NO:89) and 5′-TTAAGTCTCCTTCGTCTTTGGTGTAG-3′ (SEQ ID NO:90) and 1 μl of BD AdvantageTM 2 Polymerase Mix (Clontech Laboratories, Inc.), and was amplified in a thermocycler during 30 cycles of the following program: 94° C. for 30 seconds, 58° C. for 30 seconds, and 72° C. for one minute 30 seconds. The PCR product was purified, and subsequently cloned into the pYES2.1/V5-His-TOPO expression vector (Invitrogen).
Expression of TpLPCAT in yeast: The TpLPCAT in pYES2.1/V5-His-TOPO plasmid was transformed into yeast 1 pcat mutant By02431 using the method provided by the producer's manual (Invitrogen). Yeast cells transformed with pYES2.1/V5-His-TOPO plasmid only were used as a control. Transformants were selected by growth on synthetic complete medium lacking uracil (SC-ura), supplemented with 2% (w/v) glucose. The colonies were transferred into liquid SC-ura with 2% (w/v) glucose and grown at 28° C. overnight. The overnight cultures were diluted to an OD 0.4 in induction medium (SC-ura+2% Galactose+1% Raffinose), and were induced by incubating at 28° C. for 24 hours. The yeast cells were collected and broken using glass beads. The protein concentrations in the lysates were normalized using the Biorad assay (Bradford 1976) and then assayed for LPCAT activity.
Identification of LPCAT from the Algae Thalassiosira pseudonana 
Isolation of the LPCAT cDNA from T. pseudonana A full-length T. pseudonana LPCAT cDNA clone was amplified by PCR from an algae cDNA library. The nucleotide sequence had an open reading frame of 1,323 bp encoding a polypeptide of 440 amino acids with a calculated molecular mass of 49.75 kD.
Expression of TpLPCAT in Yeast: To confirm the function of the protein encoded by the TpLPCAT, the full-length coding region of TpLPCAT was cloned into a yeast expression vector pYES2.1/V5-His-TOPO under the control of the galactose-inducible GAL1 promoter, and the construct was used to transform a LPCAT-deficient yeast strain By02431(a yeast 1 pcat strain). Yeast cells harboring an empty pYES2.1 vector plasmid were used as a control. We also discovered that the yeast 1 pcat strain is hypersensitive to lyso-PAF (lyso-Platelet-activating factor, 1-O-alkyl-sn-glycero-3-phosphocholine). Expression of the TpLPCAT in yeast 1 pcat mutant was able to overcome lyso-PAF the sensitivity of the 1 pcat mutant (FIG. 4).
The microsomal membrane fractions prepared from lysates of the induced yeast cells were assayed for LPCAT activity using 14C-labelled Lyso-PC as acceptor, and different unlabeled acyl-CoAs as acyl donors. Under our assay conditions, expression of the TpLPCAT in yeast lpcat mutant resulted in a restoration of LPCAT function and produced a recombinant LPCAT protein capable of incorporating a range of different acyl-CoAs into PC including 14:0-, 16:0-, 16:1-, 18:0-, 18:1-, 18:2-, and 22:6(DHA)-, with the most preference of 18:1-CoA, and efficiently utilization of the very long chain polyunsaturated fatty acid—22:6-CoA(DHA) (FIGS. 5 and 6).
Example 8
Arabidopsis Gene Assays
Experimental Procedure
TA-cloning and yeast complementation: Total RNA was prepared from Arabidopsis seedlings using RNeasy Plant Mini Kit (Qiagen). RT-PCR of the ORFs of Arabidopsis At1g12460, At1g63050 was performed with primer pairs designed based on sequences of gene annotation available at TAIR (The Arabidopsis Information Resources). The cDNA was cloned into vector pYES2.1 using pYES2.1 TOPO TA Cloning Kit according to the manufacturer's protocol (Invitrogen). Correctly-oriented positive colonies were identified through double digestion with restriction enzyme, followed by verification through DNA sequencing. The construct was introduced into yeast strain YOR175c, BY02431. Yeast extract, Yeast Nitrogen Base, Bacto-peptone, and Bacto-agar were purchased from DIFCOTM, D-glucose, D-galactose and D-raffinose were from Sigma. SC minimal medium and plates was prepared according to Invitrogen's recipe described for the pYES2.1 TOPO TA Cloning Kit.
Lyso-PAF sensitivity: Yeast strains BY02431 carrying pYES 2.1-AtLPCATs or the empty vector were first grown in 15 ml of SC-Leu-His-ura medium containing 2% glucose. Yeast transformant strains of AtLPCATs were first grown in YPD overnight. Protein expression induction were carried out by protocol described in Invitrogen manufacturer manual for yeast expression vector pYES2.1. After 12 hours induction, 5 μl cultures were inoculated onto YPD plate with 10 μg/ml lysoPAF. The plates were incubated at 28° C. for two days. The final lysoPAF is 10/ml.
In Vitro Assay:
Yeast strains BY02431 carrying pYES 2.1-AtLPCATs (or the empty vector) were first grown in 15 ml of SC-Leu-His-ura medium containing 2% glucose. Yeast transformant strains of AtLPCATs were first grown in YPD overnight. Protein expression induction was carried out by protocol described in Invitrogen manufacturer manual for yeast expression vector pYES2.1. After 24 hours of growth in the galactose induction conditions, the cells were washed first with distilled water and then with wall-breaking buffer (50 mM sodium phosphate, pH7.4; 1 mM EDTA; 1 mM PMSF; 5% glycerol) and spun down at 4,000 rpm (Eppendorf Centrifuge 5145C) to re-pellet cells. The cells, resuspended in 1 ml cell wall-breaking buffer, were shaken vigorously in the presence of acid-washed glass beads (diameter 0.5 mm) in a mini-bead beater at 5,000 rpm for three 1-minute intervals. The resultant homogenate was centrifuged at 1,500 g for five minutes at 4° C. The supernatant was decanted for in vitro assay. Protein concentration was measured using Bio-Rad Protein Assay Kit for final AtSAT1 activity calculation.
AtLPCAT substrate specificity was determined by counting incorporation of 14C-labeled lysophosphatidylcholine or 14C-labeled palmitoyl-CoA into phosphatidylcholine. All assays were performed at least twice. 200 ml reaction mixture contained 50 mg microsomal protein, 50 mM acyl-CoA and 45 mM palmitoyl-PC, pH7.4. 14C-lysophosphatidylcholine (1.4 nCi/nmol) or 14C-palmityl-CoA (5.5 nCi/nmol) was used to assess fatty-CoA or lyso-lipid substrate specificity. Reaction was allowed for ten minutes at 30° C. All radiolabel chemicals for these assays were purchased from ARC, Inc.
Lyso-PAF sensitivity test (FIG. 7): The yeast 1 pcat strain is deficient in its endogenous LPCAT and hypersensitive to lyso-PAF (lyso-Platelet-activating factor, 1-O-alkyl-sn-glycero-3-phosphocholine). The 1 pcat yeast mutant is incapable of growth in the presence of 10 ug/ml lyso-PAF (lyso-Platelet-activating factor, 1-O-alkyl-sn-glycero-3-phosphocholine). However, when the Arabidopsis LPCAT genes, At1g12640 and At1g63050, were introduced into the yeast mutant, the transformants could survive on lyso-PAF-containing YPD plate. These results indicated that the Arabidopsis genes encode for LPCAT.
In vitro enzyme characterization with the yeast cell free lysate expressing the Arabidopsis LPCATs was further conducted.
Lyso-lipid substrate specificity (FIG. 8): LPA (lysophosphatidic acid), LPC (lysophosphatidic choline), LPE (lysophosphatidylethanolamine), LPG (lysophosphatidylglycerol), LPI (lysophosphatidyl inositol) and LPS (lysophosphatidyl serine) were first tested as substrates to compare their acyltransferase activity. The results clearly showed that At1g12640 and At1g63050 both exhibited high activity towards LPC (FIG. 8).
Example 9
By NCBI-BLASTp search with default Algorithm parameters (expect threshold=10; word size=3; matrix=BLOSUM62; gap costs=existence: 11 Extension: 1; compositional adjustments=composition-based statistics) following sequences (E value<5e18) from various organisms including human and mouse were identified as YOR175cp homologs.
SEQ ID NO:87: Human_XP—001129292 protein sequence, PREDICTED: similar to O-acyltransferase (membrane bound) domain containing 2 [Homo sapiens]. 
SEQ ID NO:93: Human_XP—001129292 CDS sequence







    ATGGTGATGATGATGATGATGAAGGTGCTGCTGCTGCTGATGAAGC


 


AAAGGGGAGCCGGTCTCCCTGCGCCCGCGGGCGTCGAACCCAGGCCCAGC


 


TCTCACCACCCAAAGGCCCGGGTGCGGCTGCAGGGTGACGAAAGCGTCAG


 


ACCCCGGGGCTGCTCTCAGCTTTGGGCTTTCACCCGGCACTCTCCCAGAC


 


AAAGGGGCTTCTCAGCCAGGTCGCTGTTTTGGTTTGTCGTCCTCCCAGCC


 


CCCACCTTCGTCCCCAACTTCCCCTGGCGCTGGCTCGGCGGCGTCCCTCA


 


CATCGTCCCTCCGGCCGCCACCCCGGGCCCCTTTGTTGTCTGCCGTCTCT


 


CCCAGAGAGGGGTGGGGGGCCGCGACATTCCAGGGAGGAGAAACCGAGGA


 


GTGAGGGGCAAAGACGCTCTTCCATGCTCTCACCCGAGGAGCGCGCCCCA


 


CGACGCTGGCCAGCCGTTCTCCGGCGACGCCCGCCATCCCCGGGCCGAGC


 


GGGAGGTGGGCCGGGCGTTGTTGCCGGCGACAGCCCCCGGGGAGGGTGGT


 


CGTATGGGCGTGCGGGTGTGCATGCGGTCCCTGCCCTTCGCGGCAGCGGC


 


GCTCGGATCCGGTGGTCGGGTCCCGGAGCAGCCCCCGGTGCGCATGGACC


 


GGGTTGTGGAAAGGGTGCGGAAGGCTGCGCTTTGGGGAGCCTGGCGTGGT


 


GCTGCCTGCCCCGCGCGCGCCTCTGAGCGACCCCCGGAGAGGCTGATGCA


 


TGGGTCTGGGGATGGGCTGCTTGGCTTCTCATTTGTCAGAGCAAGCTTGA


 


CAGTGTTTGGAGAGGAAGCAGGCCCATCCTTTCTATTGGCAGTTCTCTGT


 


GCTGTTGTCTGGGGAGGAAGAGGAGAGGATGTTGTGTCTGATGTACAGGC


 


TTGTCCTGCAGAACAGGGCTTCTTGCTGGCTGAACCCAGTGTATTTGGTG


 


TCAACTTTGTAGTGTGCCAACTCTTTGCCTTGCTAGCAGCCATTTGGTTT


 


CGAACTTATCTACATTCAAGCAAAACTAGCTCTTTTATAAGACATGTAGT


 


TGCTACCCTTTTGGGCCTTTATCTTGCACTTTTTTGCTTTGGATGGTATG


 


CCTTACACTTTCTTGTACAAAGTGGAATTTCCTACTGTATCATGATCATC


 


ATAGGAGTGGAGAACATGCACAATTACTGCTTTGTGTTTGCTCTGGGATA


 


CCTCACAGTGTGCCAAGTTACTCGAGTCTATATCTTTGACTATGGACAAT


 


ATTCTGCTGATTTTTCAGGCCCAATGATGATCATTACTCAGAAGATCACT


 


AGTTTGGCTTGCGAAATTCATGATGGGATGTTTCGGAAGGATGAAGAACT


 


GACTTCCTCACAGAGGGATTTAGCTGTAAGGCGCATGCCAAGCTTACTGG


 


AGTATTTGAGTTACAACTGTAACTTCATGGGGATCCTGGCAGGCCCACTT


 


TGCTCTTACAAAGACTACATTACTTTCATTGAAGGCAGATCATACCATAT


 


CACACAATCTGGTGAAAATGGAAAAGAAGAGACACAGTATGAAAGAACAG


 


AGCCATCTCCAAATACTGCGGTTGTTCAGAAGCTCTTAGTTTGTGGGCTG


 


TCCTTGTTATTTCACTTGACCATCTGTACAACATTACCTGTGGAGTACAA


 


CATTGATGAGCATTTTCAAGCTACAGCTTCGTGGCCAACAAAGATTATCT


 


ATCTGTATATCTCTCTTTTGGCTGCCAGACCCAAATACTATTTTGCATGG


 


ACGCTAGCTGATGCCATTAATAATGCTGCAGGCTTTGGTTTCAGAGGGTA


 


TGACGAAAATGGAGCAGCTCGCTGGGACTTAATTTCCAATTTGAGAATTC


 


AACAAATAGAGATGTCAACAAGTTTCAAGATGTTTCTTGATAATTGGAAT


 


ATTCAGACAGCTCTTTGGCTCAAAAGGGTGTGTTATGAACGAACCTCCTT


 


CAGTCCAACTATCCAGACGTTCATTCTCTCTGCCATTTGGCACGGGGTAT


 


ACCCAGGATATTATCTAACGTTTCTAACAGGGGTGTTAATGACATTAGCA


 


GCAAGAGCTATGAGAAATAACTTTAGACATTATTTCATTGAACCTTCCCA


 


ACTGAAATTATTTTATGATGTTATAACATGGATAGTAACTCAAGTAGCAA


 


TAAGTTACACAGTTGTGCCATTTGTGCTTCTTTCTATAAAACCATCACTC


 


ACGTTTTACAGCTCCTGGTATTATTGCCTGCACATTCTTGGTATCTTAGT


 


ATTATTGTTGTTGCCAGTGAAAAAAACTCAAAGAAGAAAGAATACACATG


 


AAAACATTCAGCTCTCACAATCCAAAAAGTTTGATGAAGGAGAAAATTCT


 


TTGGGACAGAACAGTTTTTCTACAACAAACAATGTTTGCAATCAGAATCA


 


AGAAATAGCCTCGAGACATTCATCACTAAAGCAGTGA






SEQ ID NO:85: Human_NP—005759 protein sequence, O-acyltransferase (membrane bound) domain containing 5 [Homo sapiens]:








    MASSAEGDEGTVVALAGVLQSGFQELSLNKLATSLGASEQALRLIISIFLGYPFAL



 


FYRHYLFYKETYLIHLFHTFTGLSIAYFNFGNQLYHSLLCIVLQFLILRLMGRTITAVLTTF


 


CFQMAYLLAGYYYTATGNYDIKWTMPHCVLTLKLIGLAVDYFDGGKDQNSLSSEQQK


 


YAIRGVPSLLEVAGFSYFYGAFLVGPQFSMNHYMKLVQGELIDIPGKIPNSIIPALKRLSL


 


GLFYLVGYTLLSPHITEDYLLTEDYDNHPFWFRCMYMLIWGKFVLYKYVTCWLVTEGV


 


CILTGLGFNGFEEKGKAKWDACANMKVWLFETNPRFTGTIASFNINTNAWVARYIFKRL


 


KFLGNKELSQGLSLLFLALWHGLHSGYLVCFQMEFLIVIVERQAARLIQESPTLSKLAAIT


 


VLQPFYYLVQQTIHWLFMGYSMTAFCLFTWDKWLKVYKSIYFLGHIFFLSLLFILPYIHK


 


AMVPRKEKLKKME






SEQ ID NO:94: Human_NP—005759 cDNA sequence







    ATGGCGTCCTCAGCGGAGGGGGACGAGGGGACTGTGGTGGCGCTGG


 


CGGGGGTTCTGCAGTCGGGTTTCCAGGAGCTGAGCCTTAACAAGTTGGCG


 


ACGTCCCTGGGCGCGTCAGAACAGGCGCTGCGGCTGATCATCTCCATCTT


 


CCTGGGTTACCCCTTTGCTTTGTTTTATCGGCATTACCTTTTCTACAAGG


 


AGACCTACCTCATCCACCTCTTCCATACCTTTACAGGCCTCTCAATTGCT


 


TATTTTAACTTTGGAAACCAGCTCTACCACTCCCTGCTGTGTATTGTGCT


 


TCAGTTCCTCATCCTTCGACTAATGGGCCGCACCATCACTGCCGTCCTCA


 


CTACCTTTTGCTTCCAGATGGCCTACCTTCTGGCTGGATACTATTACACT


 


GCCACCGGCAACTACGATATCAAGTGGACAATGCCACATTGTGTTCTGAC


 


TTTGAAGCTGATTGGTTTGGCTGTTGACTACTTTGACGGAGGGAAAGATC


 


AGAATTCCTTGTCCTCTGAGCAACAGAAATATGCCATACGTGGTGTTCCT


 


TCCCTGCTGGAAGTTGCTGGTTTCTCCTACTTCTATGGGGCCTTCTTGGT


 


AGGGCCCCAGTTCTCAATGAATCACTACATGAAGCTGGTGCAGGGAGAGC


 


TGATTGACATACCAGGAAAGATACCAAACAGCATCATTCCTGCTCTCAAG


 


CGCCTGAGTCTGGGCCTTTTCTACCTAGTGGGCTACACACTGCTCAGCCC


 


CCACATCACAGAAGACTATCTCCTCACTGAAGACTATGACAACCACCCCT


 


TCTGGTTCCGCTGCATGTACATGCTGATCTGGGGCAAGTTTGTGCTGTAC


 


AAATATGTCACCTGTTGGCTGGTCACAGAAGGAGTATGCATTTTGACGGG


 


CCTGGGCTTCAATGGCTTTGAAGAAAAGGGCAAGGCAAAGTGGGATGCCT


 


GTGCCAACATGAAGGTGTGGCTCTTTGAAACAAACCCCCGCTTCACTGGC


 


ACCATTGCCTCATTCAACATCAACACCAACGCCTGGGTGGCCCGCTACAT


 


CTTCAAACGACTCAAGTTCCTTGGAAATAAAGAACTCTCTCAGGGTCTCT


 


CGTTGCTATTCCTGGCCCTCTGGCACGGCCTGCACTCAGGATACCTGGTC


 


TGCTTCCAGATGGAATTCCTCATTGTTATTGTGGAAAGACAGGCTGCCAG


 


GCTCATTCAAGAGAGCCCCACCCTGAGCAAGCTGGCCGCCATTACTGTCC


 


TCCAGCCCTTCTACTATTTGGTGCAACAGACCATCCACTGGCTCTTCATG


 


GGTTACTCCATGACTGCCTTCTGCCTCTTCACGTGGGACAAATGGCTTAA


 


GGTGTATAAATCCATCTATTTCCTTGGCCACATCTTCTTCCTGAGCCTAC


 


TATTCATATTGCCTTATATTCACAAAGCAATGGTGCCAAGGAAAGAGAAG


 


TTAAAGAAGATGGAATAA






SEQ ID NO:95: Human_NP—077274 protein sequence, leukocyte receptor cluster (LRC) member 4 protein [Homo sapiens]:








     MSPEEWTYLVVLLISIPIGFLFKKAGPGLKRWGAAAVGLGLTLFTCGPHTLHSLV



 


TILGTWALIQAQPCPCHALALAWTFSYLLFFRALSLLGLPTPTPFTNAVQLLLTLKLVSLA


 


SEVQDLHLAQRKEMASGFSKGPTLGLLPDVPSLMETLSYSYCYVGIMTGPFFRYRTYLD


 


WLEQPFPGAVPSLRPLLRRAWPAPLFGLLFLLSSHLFPLEAVREDAFYARPLPARLFYMI


 


PVFFAFRMRFYVAWIAAECGCIAAGFGAYPVAAKARAGGGPTLQCPPPSSPEKAASLEY


 


DYETIRNIDCYSTDFCVRVRDGMRYWNMTVQWWLAQYIYKSAPARSYVLRSAWTMLL


 


SAYWHGLHPGYYLSFLTIPLCLAAEGRLESALRGRLSPGGQKAWDWVHWFLKMRAYD


 


YMCMGFVLLSLADTLRYWASIYFCIHFLALAALGLGLALGGGSPSRRKAASQPTSLAPE


 


KLREE






SEQ ID NO:96: Human_NP—077274 cDNA sequence







    ATGTCGCCTGAAGAATGGACGTATCTAGTGGTTCTTCTTATCTCCA


 


TCCCCATCGGCTTCCTCTTTAAGAAAGCCGGTCCTGGGCTGAAGAGATGG


 


GGAGCAGCCGCTGTGGGCCTGGGGCTCACCCTGTTCACCTGTGGCCCCCA


 


CACTTTGCATTCTCTGGTCACCATCCTCGGGACCTGGGCCCTCATTCAGG


 


CCCAGCCCTGCCCCTGCCACGCCCTGGCTCTGGCCTGGACTTTCTCCTAT


 


CTCCTGTTCTTCCGAGCCCTCAGCCTCCTGGGCCTGCCCACTCCCACGCC


 


CTTCACCAATGCCGTCCAGCTGCTGCTGACGCTGAAGCTGGTGAGCCTGG


 


CCAGTGAAGTCCAGGACCTGCATCTGGCCCAGAGGAAGGAAATGGCCTCA


 


GGCTTCAGCAAGGGGCCCACCCTGGGGCTGCTGCCCGACGTGCCCTCCCT


 


GATGGAGACACTCAGCTACAGCTACTGCTACGTGGGAATCATGACAGGCC


 


CGTTCTTCCGCTACCGCACCTACCTGGACTGGCTGGAGCAGCCCTTCCCC


 


GGGGCAGTGCCCAGCCTGCGGCCCCTGCTGCGCCGCGCCTGGCCGGCCCC


 


GCTCTTCGGCCTGCTGTTCCTGCTCTCCTCTCACCTCTTCCCGCTGGAGG


 


CCGTGCGCGAGGACGCCTTCTACGCCCGCCCGCTGCCCGCCCGCCTCTTC


 


TACATGATCCCCGTCTTCTTCGCCTTCCGCATGCGCTTCTACGTGGCCTG


 


GATTGCCGCCGAGTGCGGCTGCATTGCCGCCGGCTTTGGGGCCTACCCCG


 


TGGCCGCCAAAGCCCGGGCCGGAGGCGGCCCCACCCTCCAATGCCCACCC


 


CCCAGCAGTCCGGAGAAGGCGGCTTCCTTGGAGTATGACTATGAGACCAT


 


CCGCAACATCGACTGCTACAGCACAGATTTCTGCGTGCGGGTGCGCGATG


 


GCATGCGGTACTGGAACATGACGGTGCAGTGGTGGCTGGCGCAGTATATC


 


TACAAGAGCGCACCTGCCCGTTCCTATGTCCTGCGGAGCGCCTGGACCAT


 


GCTGCTGAGCGCCTACTGGCACGGCCTCCACCCGGGCTACTACCTGAGCT


 


TCCTGACCATCCCGCTGTGCCTGGCTGCCGAGGGCCGGCTGGAGTCAGCC


 


CTGCGGGGGCGGCTGAGCCCAGGGGGCCAGAAGGCCTGGGACTGGGTGCA


 


CTGGTTCCTGAAGATGCGCGCCTATGACTACATGTGCATGGGCTTCGTGC


 


TGCTCTCCTTGGCCGACACCCTTCGGTACTGGGCCTCCATCTACTTCTGT


 


ATCCACTTCCTGGCCCTGGCAGCCCTGGGGCTGGGGCTGGCTTTAGGTGG


 


GGGCAGCCCCAGCCGGCGGAAGGCAGCATCCCAGCCCACCAGCCTTGCCC


 


CGGAGAAGCTCCGGGAGGAGTAA






SEQ ID NO:97: Human_NP—620154 protein sequence, O-acyltransferase (membrane bound) domain containing 2 [Homo sapiens]:








    MATTSTTGSTLLQPLSNAVQLPIDQVNFVVCQLFALLAAIWFRTYLHSSKTSSFIR



 


HVVATLLGLYLALFCFGWYALHFLVQSGISYCIMIIIGVENMHNYCFVFALGYLTVCQV


 


TRVYIFDYGQYSADFSGPMMIITQKITSLACEIHDGMFRKDEELTSSQRDLAVRRMPSLL


 


EYLSYNCNFMGILAGPLCSYKDYITFIEGRSYHITQSGENGKEETQYERTEPSPNTAVVQ


 


KLLVCGLSLLFHLTICTTLPVEYNIDEHFQATASWPTKIIYLYISLLAARPKYYFAWTLAD


 


AINNAAGFGFRGYDENGAARWDLISNLRIQQIEMSTSFKMFLDNWNIQTALWLKRVCY


 


ERTSFSPTIQTFILSAIWHGVYPGYYLTFLTGVLMTLAARAMRNNFRHYFIEPSQLKLFY


 


DVITWIVTQVAISYTVVPFVLLSIKPSLTFYSSWYYCLHILGILVLLLLPVKKTQRRKNTH


 


ENIQLSQSKKFDEGENSLGQNSFSTTNNVCNQNQEIASRHSSLKQ






SEQ ID NO:98: Human_NP—620154 cDNA sequence







    ATGGCCACCACCAGCACCACGGGCTCCACCCTGCTGCAGCCCCTCA


 


GCAACGCCGTGCAGCTGCCCATCGACCAGGTCAACTTTGTAGTGTGCCAA


 


CTCTTTGCCTTGCTAGCAGCCATTTGGTTTCGAACTTATCTACATTCAAG


 


CAAAACTAGCTCTTTTATAAGACATGTAGTTGCTACCCTTTTGGGCCTTT


 


ATCTTGCACTTTTTTGCTTTGGATGGTATGCCTTACACTTTCTTGTACAA


 


AGTGGAATTTCCTACTGTATCATGATCATCATAGGAGTGGAGAACATGCA


 


CAATTACTGCTTTGTGTTTGCTCTGGGATACCTCACAGTGTGCCAAGTTA


 


CTCGAGTCTATATCTTTGACTATGGACAATATTCTGCTGATTTTTCAGGC


 


CCAATGATGATCATTACTCAGAAGATCACTAGTTTGGCTTGCGAAATTCA


 


TGATGGGATGTTTCGGAAGGATGAAGAACTGACTTCCTCACAGAGGGATT


 


TAGCTGTAAGGCGCATGCCAAGCTTACTGGAGTATTTGAGTTACAACTGT


 


AACTTCATGGGGATCCTGGCAGGCCCACTTTGCTCTTACAAAGACTACAT


 


TACTTTCATTGAAGGCAGATCATACCATATCACACAATCTGGTGAAAATG


 


GAAAAGAAGAGACACAGTATGAAAGAACAGAGCCATCTCCAAATACTGCG


 


GTTGTTCAGAAGCTCTTAGTTTGTGGGCTGTCCTTGTTATTTCACTTGAC


 


CATCTGTACAACATTACCTGTGGAGTACAACATTGATGAGCATTTTCAAG


 


CTACAGCTTCGTGGCCAACAAAGATTATCTATCTGTATATCTCTCTTTTG


 


GCTGCCAGACCCAAATACTATTTTGCATGGACGCTAGCTGATGCCATTAA


 


TAATGCTGCAGGCTTTGGTTTCAGAGGGTATGACGAAAATGGAGCAGCTC


 


GCTGGGACTTAATTTCCAATTTGAGAATTCAACAAATAGAGATGTCAACA


 


AGTTTCAAGATGTTTCTTGATAATTGGAATATTCAGACAGCTCTTTGGCT


 


CAAAAGGGTGTGTTATGAACGAACCTCCTTCAGTCCAACTATCCAGACGT


 


TCATTCTCTCTGCCATTTGGCACGGGGTATACCCAGGATATTATCTAACG


 


TTTCTAACAGGGGTGTTAATGACATTAGCAGCAAGAGCTATGAGAAATAA


 


CTTTAGACATTATTTCATTGAACCTTCCCAACTGAAATTATTTTATGATG


 


TTATAACATGGATAGTAACTCAAGTAGCAATAAGTTACACAGTTGTGCCA


 


TTTGTGCTTCTTTCTATAAAACCATCACTCACGTTTTACAGCTCCTGGTA


 


TTATTGCCTGCACATTCTTGGTATCTTAGTATTATTGTTGTTGCCAGTGA


 


AAAAAACTCAAAGAAGAAAGAATACACATGAAAACATTCAGCTCTCACAA


 


TCCAAAAAGTTTGATGAAGGAGAAAATTCTTTGGGACAGAACAGTTTTTC


 


TACAACAAACAATGTTTGCAATCAGAATCAAGAAATAGCCTCGAGACATT


 


CATCACTAAAGCAGTGA






SEQ ID NO:88: Human_XP—001131044 protein sequence, PREDICTED: similar to O-acyltransferase (membrane bound) domain containing 1 isoform 1
SEQ ID NO:99: Human_XP—001131044 cDNA sequence







    ATGGTGAATTTTGTGGTATGCCAGCTTGTTGCTCTGTTTGCTGCTT


 


TCTGGTTTCGCATCTACTTACGTCCTGGTACAACCAGCTCTGATGTCCGG


 


CATGCGGTTGCCACCATTTTTGGCATCTATTTTGTCATCTTTTGTTTCGG


 


CTGGTACTCTGTGCATCTTTTTGTGCTGGTGTTAATGTGCTATGCAATCA


 


TGGTCACTGCTAGTGTATCCAATATTCACAGATATTCCTTTTTTGTAGCA


 


ATGGGATATCTTACAATATGCCACATCAGCCGAATATACATCTTCCACTA


 


TGGAATTCTCACTACGGATTTTTCTGGGCCTCTGATGATTGTCACTCAGA


 


AGATCACAACCTTGGCATTCCAGGTTCATGATGGATTAGGTCGAAGAGCT


 


GAAGACCTTTCTGCTGAACAACATCGACTTGCTATCAAAGTGAAACCCTC


 


TTTTTTGGAATACTTAAGTTACCTTCTCAATTTCATGAGTGTCATAGCTG


 


GTCCTTGTAACAATTTCAAGGACTACATAGCCTTCATTGAGGGGAAGCAT


 


ATACACATGAAGTTGCTGGAGGTGAACTGGAAGCGAAAAGGTTTCCACAG


 


CTTGCCAGAACCTTCTCCCACAGGAGCTGTGATACACAAGTTGGGCATCA


 


CCTTGGTGTCTCTCCTTTTGTTTTTGACGCTAACGAAGACCTTTCCTGTC


 


ACCTGCCTTGTGGATGACTGGTTTGTCCATAAAGCAAGCTTTCCGGCTCG


 


ACTCTGCTACTTATATGTTGTCATGCAAGCCTCAAAGCCCAAGTATTACT


 


TTGCATGGACATTAGCTGATGCAGTGAATAACGCAGCTGGCTTTGGGTTC


 


AGCGGAGTGGATAAGAATGGGAATTTCTGTTGGGATCTGCTTTCGAACCT


 


AAACATCTGGAAAATTGAGACTGCCACAAGTTTCAAAATGTACTTGGAAA


 


ACTGGAATATTCAGACAGCTACTTGGCTAAAGTGTGTGTGCTATCAGCGG


 


GTTCCATGGTACCCCACGGTGCTAACCTTCATCCTGTCTGCTTTGTGGCA


 


TGGTGTCTACCCTGGATACTATTTTACCTTCTTAACTGGAATTCTTGTCA


 


CATTAGCAGCTAGAGCGGTCAGGAACAACTACAGACATTACTTCCTTTCT


 


TCAAGAGCTCTCAAGGCTGTGTATGATGCAGGCACCTGGGCCGTCACTCA


 


GCTGGCTGTCTCTTACACGGTAGCACCCTTTGTGATGTTGGCAGTTGAAC


 


CGACCATCAGCTTATACAAGTCCATGTACTTTTATTTGCACATCATAAGT


 


CTCCTGATAATACTATTTCTGCCAATGAAACCACAAGCTCATACGCAAAG


 


GCGGCCTCAGACTCTGAACTCTATTAATAAGAGAAAAACAGATTGA






SEQ ID NO:88: Human_XP—001125855 protein sequence, PREDICTED: similar to O-acyltransferase (membrane bound) domain containing 2 [Homo sapiens]








    MVNFVVCQLVALFAAFWFRIYLRPGTTSSDVRHAVATIFGIYFVIFCFGWYSVHL



 


FVLVLMCYAIMVTASVSNIHRYSFFVAMGYLTICHISRIYIFHYGILTTDFSGPLMIVTQKI


 


TTLAFQVHDGLGRRAEDLSAEQHRLAIKVKPSFLEYLSYLLNFMSVIAGPCNNFKDYIAF


 


IEGKHIHMKLLEVNWKRKGFHSLPEPSPTGAVIHKLGITLVSLLLFLTLTKTFPVTCLVDD


 


WFVHKASFPARLCYLYVVMQASKPKYYFAWTLADAVNNAAGFGFSGVDKNGNFCWD


 


LLSNLNIWKIETATSFKMYLENWNIQTATWLKCVCYQRVPWYPTVLTFILSALWHGVY


 


PGYYFTFLTGILVTLAARAVRNNYRHYFLSSRALKAVYDAGTWAVTQLAVSYTVAPFV


 


MLAVEPTISLYKSMYFYLHIISLLIILFLPMKPQAHTQRRPQTLNSINKRKTD






SEQ ID NO:100: Human_XP—001125855 cDNA sequence







    ATGTTCTTTAAAAAATTATCTTGCAGGTTCTGCATCACTCTTTCTT


 


CTCTCATGCTCTTGACCCAGAGGGTCACGTCCCTCTCTCTGGACATTTGT


 


GAGGGGAAAGTGAAGGCAGCATCTGGAGGCTTCAGGAGCAGGAGCTCTTT


 


GTCTGAGCATGTGTGTAAGGCACTGCCCTATTTCAGCTACTTGCTCTTTT


 


TCCCTGCTCTCCTGGGAGGCTCTCTGTGCTCCTTCCAGCGATTTCAGGCT


 


CGTGTTCAAGGGTCCAGTGCTTTGCATCCCAGACACTCTTTCTGGGCTCT


 


GAGCTGGAGGGGTCTGCAGATTCTTGGACTAGAATGCCTAAACGTGGCAG


 


TGAGCAGGGTGGTGGATGCAGGAGCGGGACTGACTGATTGCCAGCAATTC


 


GAGTGCATCTATGTCGTGTGGACCACAGCTGGGCTTTTCAAGCTCACCTA


 


CTACTCCCACTGGATCCTGGACGACTCCCTCCTCCACGCAGCGGGCTTTG


 


GGCCTGAGCTTGGTCAGAGCCCTGGAGAGGAGGGATATGTCCCCGATGCA


 


GACATCTGGACCCTGGAAAGAACCCACAGGATATCTGTGTTCTCAAGAAA


 


GTGGAACCAAAGCACAGCTCGATGGCTCCGACGGCTTGTATTCCAGCACA


 


GCAGGGCTTGGCCGTTGTTGCAGACATTTGCCTTCTCTGCCTGGTGGCAT


 


GGACTCCATCCAGGACAGGTGTTTGGTTTCGTTTGCTGGGCCGTGATGGT


 


GGAAGCTGACTACCTGATTCACTCCTTTGCCAATGAGTTTATCAGATCCT


 


GGCCGATGAGGCTGTTCTATAGAACCCTCACCTGGGCCCACACCCAGTTG


 


ATCATTGCCTACATCATGCTGGCTGTGGAGGTCAGGAGTCTCTCCTCTCT


 


CTGGTTGCTCTGTAATTCGTACAACAGTGTCTTTCCCATGGTGTACTGTA


 


TTCTGCTTTTGCTATTGGCGAAGAGAAAGCACAAATGTAACTGA






SEQ ID NO:43: Mouse_NP—080313 deduced protein sequence, O-acyltransferase (membrane bound) domain containing 2 isoform a [Mus musculus]








    MATTSTTGSTLLQPLSNAVQLPIDQVNFVVCQLFALLAAVWFRTYLHSSKTSSFIR



 


HVVATLLGLYLAFFCFGWYALHFLVQSGISYCIMIIAGVESMQQCCFVFALGYLSVCQIT


 


RVYIFDYGQYSADFSGPMMIITQKITSLAYEIHDGMFRKDEELTPSQRGLAVRRMPSLLE


 


YVSYTCNFMGILAGPLCSYKDYIAFIEGRASHVAQPSENGKDEQHGKADPSPNAAVTEK


 


LLVCGLSLLFHLTISNMLPVEYNIDEHFQATASWPTKATYLYVSLLAARPKYYFAWTLA


 


DAINNAAGFGFRGYDKNGVARWDLISNLRIQQIEMSTSFKMFLDNWNIQTALWLKRVC


 


YERATFSPTIQTFFLSAIWHGVYPGYYLTFLTGVLMTLAARAVRNNFRHYFLEPPQLKLF


 


YDLITWVATQITISYTVVPFVLLSIKPSFTFYSSWYYCLHVCSILVLLLLPVKKSQRRTSTQ


 


ENVHLSQAKKFDERDNPLGQNSFSTMNNVCNQNRDTGSRHSSLTQ






SEQ ID NO:111: Mouse_NP—080313 cDNA sequence







    ATGGCCACCACCAGCACCACGGGCTCCACCCTGCTGCAGCCCCTCA


 


GCAACGCCGTGCAACTGCCCATCGATCAGGTCAACTTTGTAGTGTGCCAG


 


CTCTTTGCCTTGTTAGCAGCCGTTTGGTTTCGAACTTATCTACACTCAAG


 


CAAAACTAGCTCTTTTATCAGACACGTAGTTGCTACCCTTTTGGGCCTTT


 


ATCTTGCATTTTTTTGCTTTGGATGGTATGCCTTACACTTTCTTGTACAA


 


AGTGGGATTTCCTACTGCATCATGATCATAGCAGGAGTGGAGAGCATGCA


 


GCAATGTTGCTTTGTGTTTGCTTTGGGATACCTCTCAGTGTGTCAGATTA


 


CTAGAGTCTATATCTTTGATTATGGACAATATTCTGCTGATTTTTCAGGC


 


CCAATGATGATCATTACGCAGAAGATCACTAGTTTGGCTTACGAAATTCA


 


CGACGGGATGTTTCGGAAGGATGAAGAACTGACTCCGTCGCAGAGGGGAT


 


TAGCTGTGAGGCGCATGCCAAGTCTCCTGGAGTATGTAAGTTATACCTGC


 


AACTTCATGGGCATCCTGGCAGGCCCACTGTGCTCCTACAAAGACTACAT


 


TGCCTTCATTGAAGGCAGAGCATCCCACGTGGCACAGCCCAGTGAAAATG


 


GAAAAGACGAGCAGCATGGGAAAGCAGATCCATCTCCAAATGCAGCAGTT


 


ACGGAGAAGCTCCTGGTCTGTGGACTCTCCTTATTATTCCACCTGACCAT


 


CTCCAACATGCTACCCGTGGAGTACAACATCGATGAGCATTTCCAAGCCA


 


CTGCGTCGTGGCCGACTAAAGCCACCTATCTGTACGTCTCTCTCTTGGCT


 


GCCAGACCTAAGTACTATTTTGCATGGACCTTAGCTGACGCCATTAACAA


 


TGCTGCGGGCTTCGGTTTCAGAGGATACGACAAGAATGGAGTGGCTCGCT


 


GGGACTTAATTTCCAACTTGAGAATTCAGCAAATAGAGATGTCAACAAGT


 


TTTAAGATGTTTCTTGATAACTGGAATATCCAGACAGCTCTTTGGCTCAA


 


AAGGGTGTGCTATGAACGAGCAACCTTCAGTCCGACAATCCAGACATTCT


 


TTCTCTCTGCCATTTGGCATGGGGTCTACCCAGGATACTATCTGACATTC


 


CTAACGGGAGTGCTAATGACGTTAGCAGCTCGGGCTGTGAGAAATAACTT


 


TAGGCACTATTTCCTGGAGCCCCCTCAACTTAAGTTATTTTATGACCTCA


 


TAACCTGGGTGGCCACCCAGATAACAATAAGTTACACAGTTGTTCCGTTT


 


GTGCTCCTCTCCATAAAACCGTCGTTCACGTTTTACAGCTCCTGGTATTA


 


CTGCCTTCACGTCTGTAGTATCTTGGTGTTGCTGTTGCTGCCTGTGAAAA


 


AGTCTCAAAGAAGAACGAGCACACAGGAAAATGTTCATCTCTCACAGGCC


 


AAAAAGTTTGACGAAAGGGACAATCCTCTGGGACAGAACAGTTTTTCCAC


 


GATGAATAACGTTTGCAATCAGAACCGAGACACTGGCTCCAGACACTCGT


 


CACTAACACAGTGA






SEQ ID NO:101: Mouse_NP—084210 deduced protein sequence, leukocyte receptor cluster (LRC) member 4 [Mus musculus]








     MTPEEWTYLMVLLISIPVGFLFKKAGPGLKRWGAAAVGLGLTLFTCGPHSLHSLI



 


TILGTWALIQAQPCSCHALALAWTFSYLLFFRALSLLGLPTPTPFTNAVQLLLTLKLVSLA


 


SEVQDLHLAQRKEIASGFHKEPTLGLLPEVPSLMETLSYSYCYVGIMTGPFFRYRTYLD


 


WLEQPFPEAVPSLRPLLRRAWPAPLFGLLFLLSSHLFPLEAVREDAFYARPLPTRLFYMIP


 


VFFAFRMRFYVAWIAAECGCIAAGFGAYPVAAKARAGGGPTLQCPPPSSPEIAASLEYD


 


YETIRNIDCYGTDFCVRVRDGMRYWNMTVQWWLAQYIYKSAPFRSYVLRSAWTMLLS


 


AYWHGLHPGYYLSFMTIPLCLAAEGYLESALRRHLSPGGQKAWDWVHWFLKMRAYD


 


YMCMGFVLLSMADTLRYWASIYFWVHFLALACLGLGLVLGGGSPSKRKTPSQATSSQA


 


KEKLREE






SEQ ID NO:102: Mouse_NP—084210 deduced cDNA sequence







    ATGACACCCGAAGAATGGACATATCTAATGGTCCTTCTTATCTCCA


 


TCCCTGTTGGCTTCCTCTTTAAGAAAGCTGGACCTGGGCTGAAGAGATGG


 


GGGGCAGCAGCTGTGGGCCTGGGGCTCACCTTATTCACCTGTGGCCCCCA


 


CAGTTTGCATTCTCTGATCACCATCTTGGGAACCTGGGCCCTCATTCAGG


 


CCCAGCCCTGCTCCTGCCATGCCCTGGCTCTTGCCTGGACCTTCTCCTAT


 


CTCCTCTTCTTCCGAGCCCTCAGCCTGCTGGGCCTGCCCACTCCCACGCC


 


CTTCACCAATGCTGTCCAGCTGCTGTTGACACTGAAGTTGGTGAGTCTAG


 


CTAGTGAAGTCCAGGATCTGCATCTGGCTCAGAGAAAGGAAATAGCCTCC


 


GGCTTCCACAAGGAGCCTACGCTGGGCCTCCTGCCTGAGGTCCCCTCTTT


 


GATGGAGACACTCAGCTATAGCTACTGTTACGTGGGAATCATGACAGGCC


 


CATTCTTCCGCTACCGCACCTACCTGGATTGGCTGGAACAGCCCTTCCCG


 


GAAGCCGTGCCCAGCCTGAGGCCCCTGCTGCGCCGCGCCTGGCCAGCCCC


 


GCTCTTTGGCCTGCTCTTCCTGCTGTCCTCCCATCTCTTCCCACTGGAAG


 


CTGTGCGTGAGGACGCCTTCTACGCCCGCCCGCTGCCCACCCGCCTCTTC


 


TACATGATCCCGGTCTTCTTCGCCTTCCGCATGCGCTTCTACGTTGCCTG


 


GATTGCGGCCGAGTGCGGTTGCATTGCCGCGGGCTTCGGGGCCTACCCTG


 


TGGCTGCCAAAGCCCGGGCCGGGGGCGGCCCCACCCTCCAATGCCCACCC


 


CCTAGCAGTCCGGAGATTGCAGCTTCCCTGGAGTATGACTATGAGACCAT


 


CCGTAACATCGACTGCTATGGCACAGACTTCTGCGTGCGTGTGCGGGATG


 


GCATGCGATACTGGAACATGACCGTGCAGTGGTGGCTGGCACAGTACATC


 


TACAAGAGCGCACCTTTCCGCTCCTACGTTTTGAGGAGTGCCTGGACCAT


 


GCTGTTGAGTGCCTACTGGCATGGCCTCCACCCTGGTTACTACCTAAGCT


 


TCATGACCATCCCGCTGTGCCTGGCTGCTGAGGGCTATTTGGAGTCAGCC


 


TTGCGGAGACACCTGAGCCCCGGGGGCCAGAAAGCCTGGGACTGGGTCCA


 


CTGGTTCCTGAAGATGCGTGCCTACGACTACATGTGCATGGGCTTTGTGC


 


TCCTTTCCATGGCTGACACACTCCGGTACTGGGCCTCCATCTACTTCTGG


 


GTCCACTTTCTAGCCCTGGCTTGCTTGGGGCTGGGGCTGGTTTTGGGTGG


 


GGGCAGCCCCAGCAAGAGGAAGACACCATCCCAGGCCACCAGCAGCCAAG


 


CGAAGGAAAAGCTCCGGGAAGAGTGA






SEQ ID NO:103: Mouse_NP—660112 deduced protein sequence, membrane bound O-acyltransferase domain containing 5 [Mus musculus]








    MASTADGDMGETLEQMRGLWPGVEDLSLNKLATSLGASEQALRLIFSIFLGYPL



 


ALFYRHYLFYKDSYLIHLFHTFTGLSIAYFNFGHQFYHSLLCVVLQFLILRLMGRTVTAVI


 


TTLCFQMAYLLAGYYYTATGDYDIKWTMPHCVLTLKLIGLCIDYYDGGKDGNSLTSEQ


 


QKYAIRGVPSLLEVAGFSYFYGAFLVGPQFSMNHYMKLVRGQLTDIPGKMPNSTIPALK


 


RLSLGLVYLVGYTLLSPHITDDYLLTEDYDNRPFWFRCMYMLIWGKFVLYKYVTCWLV


 


TEGVCILSGLGFNGFDENGTVRWDACANMKVWLFETTPRFNGTIASFNINTNAWVARYI


 


FKRLKFLGNKELSQGLSLLFLALWHGLHSGYLICFQMEFLIVIVEKQVSSLIRDSPALSSL


 


ASITALQPFYYLVQQTIHWLFMGYSMTAFCLFTWDKWLKVYRSIYFLGHVFFLSLLFILP


 


YIHKAMVPRKEKLKKRE






SEQ ID NO:104: Mouse_NP—660112 deduced cDNA sequence







    ATGGCGTCTACAGCGGACGGGGACATGGGAGAGACGCTGGAGCAGA


 


TGCGGGGGCTGTGGCCGGGTGTCGAGGATCTGAGCCTTAACAAGTTGGCG


 


ACGTCTCTGGGCGCGTCGGAACAGGCGCTGCGGCTCATCTTCTCCATCTT


 


CCTGGGCTACCCGTTGGCTCTGTTTTACCGGCATTACCTTTTCTACAAGG


 


ACAGCTACCTCATCCATCTCTTCCACACCTTCACGGGCCTCTCAATTGCT


 


TATTTCAACTTTGGCCACCAGTTCTACCACTCCTTGCTATGTGTCGTGCT


 


TCAGTTCCTCATCCTGCGACTCATGGGCCGCACCGTCACTGCCGTTATTA


 


CTACCCTTTGCTTCCAGATGGCCTACCTTCTTGCCGGATATTACTACACA


 


GCCACCGGTGACTACGATATCAAGTGGACAATGCCACATTGTGTCTTGAC


 


ACTGAAGCTAATTGGGCTGTGTATTGACTACTACGATGGAGGCAAAGACG


 


GGAATTCCTTGACCTCTGAGCAACAGAAATATGCCATACGGGGTGTCCCT


 


TCATTGCTGGAAGTTGCTGGCTTCTCCTACTTCTATGGAGCCTTCTTGGT


 


AGGGCCCCAATTTTCAATGAACCACTACATGAAGCTGGTGCGGGGACAGC


 


TGACTGACATACCAGGGAAGATGCCAAACAGCACCATACCTGCTCTCAAG


 


CGCCTGAGTCTGGGCCTTGTCTACCTGGTGGGCTACACCCTGCTGAGCCC


 


CCACATCACAGACGACTATCTCCTCACAGAAGACTATGATAACCGCCCTT


 


TCTGGTTCCGCTGCATGTACATGCTGATCTGGGGCAAATTTGTGCTGTAC


 


AAATACGTCACCTGCTGGCTGGTCACAGAAGGAGTGTGCATTCTGTCGGG


 


CCTGGGCTTTAATGGCTTCGATGAAAATGGGACCGTGAGATGGGATGCCT


 


GTGCCAACATGAAAGTGTGGCTCTTTGAAACCACCCCTCGCTTCAATGGC


 


ACCATCGCCTCTTTCAACATCAATACCAATGCCTGGGTAGCCCGTTACAT


 


CTTCAAACGCCTCAAGTTCCTTGGAAATAAAGAGCTCTCACAAGGTCTCT


 


CCTTGCTGTTCTTGGCCCTCTGGCATGGCCTACACTCAGGATACCTGATT


 


TGCTTCCAGATGGAATTCCTCATTGTTATCGTGGAAAAGCAGGTCAGCAG


 


TCTAATTCGGGACAGCCCTGCCCTGAGCAGCCTGGCCTCCATCACTGCCC


 


TACAGCCCTTCTACTACTTGGTGCAACAGACCATCCACTGGCTGTTCATG


 


GGTTACTCTATGACTGCCTTCTGCCTCTTCACATGGGACAAATGGCTTAA


 


GGTGTACAGATCCATCTATTTCCTTGGACATGTCTTCTTCTTGAGCCTAC


 


TATTCATATTGCCTTATATCCACAAAGCAATGGTGCCAAGAAAAGAAAAG


 


TTAAAAAAGAGGGAATGA






SEQ ID NO:105: Mouse_NP—705774 deduce protein sequence, membrane bound O-acyltransferase domain containing 1 [M. musculus]








    MAARPPASLSYRTTGSTCLHPLSQLLGIPLDQVNFVACQLFALSAAFWFRIYLHP



 


GKASPEVRHTLATILGIYFVVFCFGWYAVHLFVLVLMCYGVMVTASVSNIHRYSFFVA


 


MGYLTICHISRIYIFHYGILTTDFSGPLMIVTQKITTLAFQVHDGLGRKAEDLSAEQHRLA


 


VKAKPSLLEYLSYHLNFMSVIAGPCNNFKDYVAFIEGRHIHMKLLEVNWTQRGFQSLPE


 


PSPMGAVIQKLCVTLMSLLLFLTLSKSFPVTFLIDDWFVHKANFLSRLWYLYVVMQAAK


 


PKYYFAWTLADAVHNAAGFGFNGMDTDGKSRWDLLSNLNIWKIETATSFKMYLENWN


 


IQTSTWLKCVCYERVPWYPTVLTFLLSALWHGVYPGYYFTFLTGVPVTLAARAVRNNY


 


RHHFLSSKARKIAYDVVTWAVTQLAVSYTAAPFVMLAVEPTISLYKSVFFFLHIICLLIIL


 


FLPIKPHQPQRQSRSPNSVKKKAD






SEQ ID NO:106: Mouse_NP—705774 cDNA sequence







    ATGGCAGCACGGCCGCCCGCCAGCCTCTCTTACCGTACCACCGGCT


 


CCACCTGCCTGCACCCGCTCAGCCAGCTCCTGGGCATCCCGCTGGATCAG


 


GTTAACTTTGTGGCTTGCCAGCTCTTTGCCTTGTCTGCTGCTTTCTGGTT


 


CAGAATCTACTTACATCCTGGTAAAGCCAGCCCTGAGGTCCGGCACACCT


 


TGGCCACCATTTTGGGCATCTATTTTGTTGTGTTTTGTTTTGGTTGGTAT


 


GCTGTACATCTCTTTGTGCTGGTGTTGATGTGTTATGGGGTCATGGTCAC


 


TGCAAGTGTATCCAATATTCACAGGTATTCCTTTTTTGTAGCCATGGGCT


 


ACCTTACGATATGCCACATCAGCCGCATTTACATCTTCCACTATGGAATT


 


CTCACTACAGATTTTTCTGGGCCCCTGATGATTGTCACTCAGAAGATCAC


 


GACGTTGGCTTTCCAAGTTCATGATGGATTGGGTCGAAAAGCTGAAGACC


 


TTTCTGCTGAGCAACACCGACTTGCTGTGAAAGCGAAGCCCTCGCTTCTG


 


GAATACTTAAGCTACCATCTCAACTTTATGAGTGTCATAGCCGGCCCTTG


 


CAACAATTTCAAGGACTACGTAGCCTTCATCGAAGGGAGACATATACACA


 


TGAAGTTGCTGGAAGTGAACTGGACGCAAAGGGGTTTCCAGAGTTTGCCA


 


GAGCCTTCTCCCATGGGAGCTGTGATACAGAAGTTGTGTGTGACCTTGAT


 


GTCTCTCCTGTTGTTTTTGACGCTCTCCAAGTCCTTTCCCGTCACCTTCC


 


TTATTGATGACTGGTTTGTACATAAGGCCAACTTTCTGAGTCGTCTCTGG


 


TACTTATATGTCGTCATGCAAGCCGCAAAGCCCAAGTATTACTTTGCGTG


 


GACATTAGCAGATGCGGTGCACAATGCAGCTGGATTCGGGTTCAATGGCA


 


TGGACACGGATGGGAAGTCTCGCTGGGATTTACTATCTAACCTGAACATC


 


TGGAAGATTGAGACTGCCACGAGTTTCAAAATGTACTTGGAAAACTGGAA


 


TATTCAGACATCTACGTGGCTGAAATGTGTGTGCTATGAGCGGGTTCCCT


 


GGTACCCCACAGTGCTCACCTTCCTCCTGTCTGCTCTGTGGCACGGCGTC


 


TACCCTGGATACTACTTCACATTCCTAACTGGAGTCCCTGTCACATTGGC


 


AGCCAGAGCGGTGAGGAACAACTACAGACACCACTTCCTCTCTTCCAAAG


 


CTCGAAAGATTGCCTATGACGTGGTGACCTGGGCTGTCACTCAGTTGGCT


 


GTCTCTTACACGGCAGCGCCTTTCGTCATGTTGGCAGTCGAGCCAACCAT


 


CAGTTTATACAAGTCCGTGTTCTTTTTTTTACACATCATATGTCTGCTGA


 


TAATCCTCTTTCTGCCAATCAAACCACACCAGCCTCAAAGGCAGTCTCGG


 


AGTCCAAATTCTGTAAAGAAGAAGGCAGACTGA






SEQ ID NO:107: Mouse_NP—001076810 deduced protein sequence, O-acyltransferase (membrane bound) domain containing 2 isoform b [M. musculus]








    MATTSTTGSTLLQPLSNAVQLPIDQVNFVVCQLFALLAAVWFRTYLHSSKTSSFIR



 


HVVATLLGLYLAFFCFGWYALHFLVQSGISYCIMIIAGVESMQHPMMIITQKITSLAYEIH


 


DGMFRKDEELTPSQRGLAVRRMPSLLEYVSYTCNFMGILAGPLCSYKDYIAFIEGRASH


 


VAQPSENGKDEQHGKADPSPNAAVTEKLLVCGLSLLFHLTISNMLPVEYNIDEHFQATA


 


SWPTKATYLYVSLLAARPKYYFAWTLADAINNAAGFGFRGYDKNGVARWDLISNLRIQ


 


QIEMSTSFKMFLDNWNIQTALWLKRVCYERATFSPTIQTFFLSAIWHGVYPGYYLTFLTG


 


VLMTLAARAVRNNFRHYFLEPPQLKLFYDLITWVATQITISYTVVPFVLLSIKPSFTFYSS


 


WYYCLHVCSILVLLLLPVKKSQRRTSTQENVHLSQAKKFDERDNPLGQNSFSTMNNVC


 


NQNRDTGSRHSSLTQ






SEQ ID NO:108: Mouse_NP—001076810 cDNA sequence







    ATGGCCACCACCAGCACCACGGGCTCCACCCTGCTGCAGCCCCTCA


 


GCAACGCCGTGCAACTGCCCATCGATCAGGTCAACTTTGTAGTGTGCCAG


 


CTCTTTGCCTTGTTAGCAGCCGTTTGGTTTCGAACTTATCTACACTCAAG


 


CAAAACTAGCTCTTTTATCAGACACGTAGTTGCTACCCTTTTGGGCCTTT


 


ATCTTGCATTTTTTTGCTTTGGATGGTATGCCTTACACTTTCTTGTACAA


 


AGTGGGATTTCCTACTGCATCATGATCATAGCAGGAGTGGAGAGCATGCA


 


GCACCCAATGATGATCATTACGCAGAAGATCACTAGTTTGGCTTACGAAA


 


TTCACGACGGGATGTTTCGGAAGGATGAAGAACTGACTCCGTCGCAGAGG


 


GGATTAGCTGTGAGGCGCATGCCAAGTCTCCTGGAGTATGTAAGTTATAC


 


CTGCAACTTCATGGGCATCCTGGCAGGCCCACTGTGCTCCTACAAAGACT


 


ACATTGCCTTCATTGAAGGCAGAGCATCCCACGTGGCACAGCCCAGTGAA


 


AATGGAAAAGACGAGCAGCATGGGAAAGCAGATCCATCTCCAAATGCAGC


 


AGTTACGGAGAAGCTCCTGGTCTGTGGACTCTCCTTATTATTCCACCTGA


 


CCATCTCCAACATGCTACCCGTGGAGTACAACATCGATGAGCATTTCCAA


 


GCCACTGCGTCGTGGCCGACTAAAGCCACCTATCTGTACGTCTCTCTCTT


 


GGCTGCCAGACCTAAGTACTATTTTGCATGGACCTTAGCTGACGCCATTA


 


ACAATGCTGCGGGCTTCGGTTTCAGAGGATACGACAAGAATGGAGTGGCT


 


CGCTGGGACTTAATTTCCAACTTGAGAATTCAGCAAATAGAGATGTCAAC


 


AAGTTTTAAGATGTTTCTTGATAACTGGAATATCCAGACAGCTCTTTGGC


 


TCAAAAGGGTGTGCTATGAACGAGCAACCTTCAGTCCGACAATCCAGACA


 


TTCTTTCTCTCTGCCATTTGGCATGGGGTCTACCCAGGATACTATCTGAC


 


ATTCCTAACGGGAGTGCTAATGACGTTAGCAGCTCGGGCTGTGAGAAATA


 


ACTTTAGGCACTATTTCCTGGAGCCCCCTCAACTTAAGTTATTTTATGAC


 


CTCATAACCTGGGTGGCCACCCAGATAACAATAAGTTACACAGTTGTTCC


 


GTTTGTGCTCCTCTCCATAAAACCGTCGTTCACGTTTTACAGCTCCTGGT


 


ATTACTGCCTTCACGTCTGTAGTATCTTGGTGTTGCTGTTGCTGCCTGTG


 


AAAAAGTCTCAAAGAAGAACGAGCACACAGGAAAATGTTCATCTCTCACA


 


GGCCAAAAAGTTTGACGAAAGGGACAATCCTCTGGGACAGAACAGTTTTT


 


CCACGATGAATAACGTTTGCAATCAGAACCGAGACACTGGCTCCAGACAC


 


TCGTCACTAACACAGTGA






SEQ ID NO:109: Mouse_XP—134120 deduced protein sequence, PREDICTED: similar to O-acyltransferase (membrane bound) domain containing 1 [M. musculus].








    MPHCLQGTASESDFSVNTARGENACILWFPWLRPSVGKPTFTLLISSASISFCPAG



 


LSTSYKKATESPVVTSLLQGHRLGTLGRTVGLTFRMDWLQLFFLHPLSFYQGAAFPFAL


 


LFNYLCILDTFSTRARYLFLLAGGGVLAFAAMGPYSLLIFIPALCAVALVSFLSPQEVHRL


 


TFFFQMGWQTLCHLGLHYTEYYLGEPPPVRFYITLSSLMLLTQRVTSLSLDICEGKVEAP


 


RRGIRSKSSFSEHLWDALPHFSYLLFFPALLGGSLCSFRRFQACVQRSSSLYPSISFRALT


 


WRGLQILGLECLKVALRSAVSAGAGLDDCQRLECIYLMWSTAWLFKLTYYSHWILDDS


 


LLHAAGFGAEAGQGPGEEGYVPDVDIWTLETTHRISLFARQWNRSTALWLRRLVFRKS


 


RRWPLLQTFAFSAWWHGLHPGQVFGFLCWSVMVKADYLIHTFANVCIRSWPLRLLYR


 


ALTWAHTQLIIAYIMLAVEGRSLSSLCQLCCSYNSLFPVMYGLLLFLLAERKDKRNSAFS


 


F






SEQ ID NO:110: Mouse_XP—134120 deduced cDNA sequence







    ATGCCACACTGCCTGCAAGGTACAGCCTCTGAGAGTGACTTTTCAG


 


TAAACACTGCGAGGGGAGAGAATGCCTGCATACTTTGGTTTCCATGGCTC


 


CGCCCCTCTGTTGGGAAGCCAACCTTTACATTGCTTATCTCCAGTGCTTC


 


CATTTCATTTTGTCCGGCAGGCCTTTCTACATCCTATAAAAAGGCTACGG


 


AGAGCCCAGTTGTGACTTCCCTTTTACAAGGGCACCGCTTAGGGACTCTA


 


GGAAGGACAGTGGGCCTCACATTCAGGATGGATTGGCTCCAGCTCTTTTT


 


TCTGCATCCTTTATCATTTTATCAAGGGGCTGCATTCCCCTTTGCGCTTC


 


TGTTTAATTATCTCTGCATCTTGGACACCTTTTCCACCCGGGCCAGGTAC


 


CTCTTTCTCCTGGCTGGAGGAGGTGTCCTGGCTTTTGCTGCCATGGGTCC


 


CTACTCTCTGCTCATCTTCATCCCTGCGCTCTGCGCTGTGGCTCTGGTCT


 


CCTTCCTCAGTCCACAGGAAGTCCATAGGCTGACCTTCTTCTTTCAGATG


 


GGCTGGCAGACCCTGTGCCATCTGGGTCTTCACTACACCGAATACTACCT


 


GGGTGAGCCTCCACCCGTGAGGTTCTACATCACTCTTTCTTCCCTCATGC


 


TCTTGACGCAGAGAGTCACATCCCTCTCACTGGACATTTGTGAAGGGAAG


 


GTGGAGGCCCCGAGGCGGGGCATCAGGAGCAAGAGTTCTTTCTCTGAGCA


 


CCTGTGGGATGCTCTACCTCATTTCAGCTACTTGCTCTTTTTCCCTGCTC


 


TCCTGGGAGGCTCCCTGTGTTCCTTCCGGAGGTTTCAGGCTTGCGTTCAA


 


AGATCAAGCTCTTTGTATCCGAGTATCTCTTTTCGGGCTCTGACCTGGAG


 


GGGTCTGCAGATTCTCGGGCTGGAGTGCCTCAAGGTGGCGCTGAGGAGCG


 


CGGTGAGTGCTGGAGCTGGACTGGATGACTGCCAGCGGCTGGAGTGCATC


 


TACCTCATGTGGTCCACAGCCTGGCTCTTTAAACTCACCTATTACTCCCA


 


TTGGATCCTGGACGACTCTCTCCTCCACGCGGCGGGCTTTGGCGCTGAGG


 


CTGGCCAGGGGCCTGGAGAGGAGGGATACGTCCCCGACGTGGACATTTGG


 


ACCCTGGAAACTACCCACAGGATCTCCCTGTTCGCCAGGCAGTGGAACCG


 


AAGCACAGCTCTGTGGCTCAGGAGGCTCGTCTTCCGGAAGAGCCGGCGCT


 


GGCCCCTGCTGCAGACATTTGCCTTCTCTGCCTGGTGGCACGGGCTCCAC


 


CCAGGTCAGGTGTTCGGCTTCCTGTGCTGGTCTGTAATGGTGAAAGCCGA


 


TTATCTGATTCACACTTTTGCCAACGTATGTATCAGATCCTGGCCCCTGC


 


GGCTGCTTTATAGAGCCCTCACTTGGGCTCATACCCAACTCATCATTGCC


 


TACATCATGCTGGCGGTGGAGGGCCGGAGCCTTTCCTCTCTCTGCCAACT


 


GTGCTGTTCTTACAACAGTCTCTTCCCTGTGATGTACGGTCTTTTGCTTT


 


TTCTGTTAGCGGAGAGAAAAGACAAACGTAACTGA






All of the above human and mouse YOR175cp homologs were aligned with YOR175cp sequence with MegAlign program of Lasergene7.0 software package (FIG. 20). Mouse proteins NP—660112 and NP—084210, human proteins NP—005759 and NP—077274 were characterized.
REFERENCES
The contents of the following references are incorporated herein in their entirety.

    Abbadi A., F. Domergue, J. Bauer, J. A. Napier, R. Welti, U. Zähinger, P. Cirpus, and E. Heinz (2004). Biosynthesis of very-long-chain polyunsaturated fatty acids in transgenic oilseeds: constraints on their accumulation. Plant Cell 16:2734-2748.
    Bechtold N., J. Ellis, and G. Pellefer (1993). In planta Agrobacterium-mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. C. R. Acad. Sci. Ser. Ill Sci. Vie, 316:1194-1199.
    Becker D., R. Brettschneider, and H. Lorz (1994). Fertile transgenic wheat from microprojectile bombardment of scutellar tissue. Plant J. 5:299-307.
    Chen X., B. A. Hyatt, M. L. Mucenski, R. J. Mason, and J. M. Shannon (2006). Identification and characterization of a lysophosphatidylcholine acyltransferase in alveolar type II cells. Proc. Natl. Acad. Sci. USA 103:11724-11729.
    Datla R, J. W. Anderson, and G. Selvaraj (1997). Plant promoters for transgene expression. Biotechnology Annual Review 3:269-296.
    DeBlock M., D. DeBrouwer, and P. Tenning (1989). Transformation of Brassica napus and Brassica oleracea using Agrobacterium tumefaciens and the expression of the bar and neo genes in the transgenic plants. Plant Physiol. 91:694-701.
    Domergue F., A. Abbadi, and E. Heinz (2005). Relief for fish stocks: oceanic fatty acids in transgenic oilseeds. Trend Plant Sci. 10:112-116.
    Galván E. M., H. Chen, and D. M. Schifferli (2007). The Psa fimbriae of Yersinia pestis interact with phosphatidylcholine on alveolar epithelial cells and pulmonary surfactant. Infect. Immun. 75:1272-1279.
    Huang Y. S., S. L. Pereira, and A. E. Leonard (2004). Enzymes for transgenic biosynthesis of long-chain polyunsaturated fatty acids. Biochimie 86:793-798.
    Katavic Y., G. W. Haughn, D. Reed, M. Martin, and L. Kunst (1994). In planta transformation of Arabidopsis thaliana. Mol. Gen. Genet. 245:363-370.
    Meyer P. (1995). Understanding and controlling transgene expression. Trends in Biotechnology 13:332-337.
    Moloney M. M., J. M. Walker, and K. K. Sharma (1989). High efficiency transformation of Brassica napus using Agrobacterium vectors. Plant Cell Rep. 8:238-242.
    Napier J. A., F. Beaudoin, L. V. Michaelson, and O. Sayanova (2004). The production of long chain polyunsaturated fatty acids in transgenic plants by reverse-engineering. Biochimic 86:785-793.
    Nehra N. S., R. N. Chibbar, N. Leung, K. Caswell, C. Mallard, L. Steinhauer, M. Baga, and K. K. Kartha (1994). Self-fertile transgenic wheat plants regenerated from isolated. scutellar tissues following microprojectile bombardment with two distinct gene constructs. Plant J 5:285-297.
    Potrykus L. (1991). Gene transfer to plants: Assessment of publish approaches and results. Annu. Rev. Plant Physiol. Plant Mol. Biol. 42:205-225.
    Pouwels et al., Cloning Vectors. A laboratory manual, Elsevier, Amsterdam (1986).
    Qi B., T. Fraser, S. Mugford, G. Dobson, O. Sayanova, J. Butler, J. A. Napier, A. K. Stobart, and C. M. Lazarus (2004). Production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in plants. Nat. Biotechnol. 22:739-745.
    Rhodes C. A., D. A. Pierce, I. J. Mettler, D. Mascarenhas, and J. J. Detmer (1988). Genetically transformed maize plants from protoplasts. Science 240:204-207.
    Sanford J. C., T. M. Klein, E. D. Wolf, and N. Allen (1987). Delivery of substances into cells and tissues using a particle bombardment process. J. Part. Sci. Technol. 5:27-37.
    Shimamoto K., R. Terada, T. Izawa, and H. Fujimoto (1989). Fertile transgenic rice plants regenerated from transformed protoplasts. Nature 335:274-276.
    Shindou H., D. Hishikawa, H. Nakanishi, T. Harayama, S. Ishii, R. Taguchi, and T. Shimizu (2007). A single enzyme catalyzes both platelet-activating factor production and membrane biogenesis of inflammatory cells: Cloning and characterization of acetyl-CoA: lyso-PAF acetyltransferase. J Biol Chem. 282:6532-6539.
    Songstad D. D., D. A. Somers, and R. J. Griesbach (1995). Advances in alternative DNA delivery techniques. Plant Cell, Tissue and Organ Culture 40:1-15.
    Tamaki H., A. Shimada, Y. Ito, M. Ohya, J. Takase, M. Miyashita, H. Miyagawa, H. Nozaki, R. Nakayama, and H. Kumagai (2007). LPT1 encodes a membrane-bound O-acyltransferase involved in the acylation of lysophospholipids in the yeast Saccharomyces cerevisiae. J. Biol. Chem. [Epub ahead of print]
    Testet E., J. Laroche-Traineau, A. Noubhani, D. Coulon, O. Bunoust, N. Camougrand, S. Manon, R. Lessire, and J. J. Bessoule (2005). Ypr140wp, “the yeast tafazzin,” displays a mitochondrial lysophosphatidylcholine (lyso-PC) acyltransferase activity related to triacylglycerol and mitochondrial lipid synthesis. Biochem. J. 387:617-626.
    Vasil I. K. (1994). Molecular improvement of cereals. Plant Mol. Biol. 5:925-937.
    Walden R. and R. Wingender (1995). Gene-transfer and plant regeneration techniques. Trends in Biotechnology 13:324-331.
    Wu G., M. Truksa, N. Datla, P. Vrinten, J. Bauer, T. Zank, P. Cirpus, E. Heinz, and X. Qiu (2005). Stepwise engineering to produce high yields of very long-chain polyunsaturated fatty acids in plants. Nat. Biotechnol. 23:1013-1017.
    Yavin E. (1976). Regulation of phospholipids metabolism in differentiating cells from rat brain cerebral hemispheres in culture. Patterns of acetylcholine phosphocholine, and choline phosphoglycerides labeling from (methyl-14C) choline. J. Biol. Chem. 251:1392-1397.

## Claims

1. A nucleic acid molecule comprising a first polynucleotide operably linked to a second, heterologous polynucleotide, wherein the first polynucleotide encodes at least one peptide selected from the group consisting of SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:81, SEQ ID NO:82, SEQ ID NO:83, and SEQ ID NO:84.

2. A method for increasing fatty acid production in a cell, the method comprising:
expressing in a cell a nucleic acid molecule encoding a lyso-phosphatidylcholine acyltransferase
comprising:
(i) the motifs of SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, and SEQ ID NO:49, or
(ii) the motifs of SEQ ID NO:81, SEQ ID NO:82, SEQ ID NO:83, and SEQ ID NO:84.

3. The method according to claim 2, further comprising isolating fatty acids from the cell.

4. The method according to claim 2, wherein the cell is a plant cell.

5. The method according to claim 2, wherein the lyso-phosphatidylcholine acyltransferase comprises SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, and SEQ ID NO:49.

6. The method according to claim 5, wherein the cell is a plant cell.

7. The method according to claim 2, wherein the lyso-phosphatidylcholine acyltransferase comprises SEQ ID NO:81, SEQ ID NO:82, SEQ ID NO:83, and SEQ ID NO:84.

8. The method according to claim 7, wherein the cell is a plant cell.

9. A method of altering oil content in a plant, the method comprising:
expressing a nucleic acid molecule in the plant to alter the oil content of the plant, wherein the nucleic acid molecule encodes a lyso-phosphatidylcholine acyltransferase comprising:
(i) the motifs of SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, and SEQ ID NO:49, or
(ii) the motifs of SEQ ID NO:81, SEQ ID NO:82, SEQ ID NO:83, and SEQ ID NO:84.

10. The method according to claim 9, wherein the lyso-phosphatidylcholine acyltransferase comprises SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, and SEQ ID NO:49.

11. The method according to claim 9, wherein the lyso-phosphatidylcholine acyltransferase comprises SEQ ID NO:81, SEQ ID NO:82, SEQ ID NO:83, and SEQ ID NO:84.

12. A process of obtaining seeds, the process comprising:
transforming a plant cell with a recombinant DNA construct comprising a polynucleotide encoding a lyso-phosphatidylcholine acyltransferase, and a promoter for driving the expression of the polynucleotide in the plant cell to form a transformed plant cell, regenerating the transformed plant cell into a transgenic plant, and selecting transgenic plants that have enhanced levels of fatty acids in the seeds compared wild-type strains of the same plant;
cultivating the transformed plant for one or more generations, to produce a genetically modified plant; and
harvesting seeds from the genetically-modified plant,
wherein the lyso-phosphatidylcholine acyltransferase comprises:
(i) the motifs of SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, and SEQ ID NO:49, or
(ii) the motifs of SEQ ID NO:81, SEQ ID NO:82, SEQ ID NO:83, and SEQ ID NO:84.

13. A seed produced by a genetically-modified plant comprising a nucleic acid molecule encoding a lyso-phosphatidylcholine acyltransferase comprising:
(i) the motifs of SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, and SEQ ID NO:49, or
(ii) the motifs of SEQ ID NO:81, SEQ ID NO:82, SEQ ID NO:83, and SEQ ID NO:84.

14. A process for obtaining oil comprising enhanced levels of fatty acids, the process comprising:
extracting oil from the seed of claim 13.