GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , and PFO

S I n Groundwate r QuarterlyGroundwate rSamplin g - Sep t 201 0 

# 3 M

GLP10-01-02 : Interi m Repor t 0 9 - Analysi s o fPFBS
. PFHS
. an d PFO S i n Groun d Wate rSample s Collecte d fro m Decatur
. A L i n Septembe r201 0
Stud yTitl e Analysi s ofPerfluorooctan e Sulfonate (PFOS) , Perfluorohexan e Sulfonate (PFHS ) an d Perfluorobutan e Sulfonate (PFBS ) i n Groundwater , Soi l an d Sedimen t forth e 3 M Decatu rPhase 3 Site-Relate d Monitorin g Progra m 

### Dat a Requiremen t EPATSC AGoo d Laborator yPractic e Standard s 4 0 CF R Par t 79 2 

### Stud yDirecto r Jaisimh a Kesar i P.E. , DE E Westo n Solutions , Inc
. 140 0 Westo n Way Westchester , PA1938 0 Phone : 610-701-376 1 

### Autho r Susa n Wol f 3 M Environmenta l Laborator y 

### Interi m Repor t Completio n Dat e

Dat e ofsignin g 

### Performin g Laborator y 3 M Environmenta l Healt h an d Safet yOperation s Environmenta l Laborator

y 3 M Center , BId g 260-05-N-1 7 Maplewood , M N 55144 

### Projec t Identificatio n GLP10-01-02-0 9 

### Tota l Numbe ro fPage s

8 3

Th e testin g reported herei n mee t th e requirement

s ofiSO/lE C 17025-200

5 "Genera l Requirement

s fo rth e Competenc

e ofTestin g an d Calibratio n laboratories" , i n accordanc e wit h th e A2L

ACertificate #2052.01
. Testin g tha

t complies wit h thi s Internationa l Standar d als o operat

e i n accordanc

e wit h IS O 9001:2000
. 

### Certificat e #2052.0 1

Page 1 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0



### Thi s pag e has bee n reserve d fo rspecifi ccountr yrequirements
.

Pag e 2 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sept 201 0



### GL P COMPLIANC E STATEMEN T Repor t Title : Interi m Repor

t 0 9 Analysi s ofPFBS , PFHS , an d PFO S i n Groun d Wate rSamples Collecte d fro m Decatur , A Li n September , 201 0 Study : Analysi s o fPerfluorooctan e Sulfonate (PFOS) , Perfluorohexan e Sulfonat e (PFHS ) an d Perfluorobutan e Sulfonat e (PFBS ) i n Groundwater , Soi l an d Sedimen t fo rth e 3 M Decatu rPhas e 3 Site-Relate d Monitorin g Program
. Thi s analytica l phas e wa s conducte d i n compliance wit h Toxi cSubstance s Contro l Act (TSCA ) Good Laborator yPractice (GLP ) Standards , 4 0 CF R 792 , wit h th e exception s liste d below :

•Thes e are environmenta l sample s where there i s n o specifi ctest substance , n o specifi ctest syste m an d n o dosin g o fa test system
. •Th e reference substance s hav e no t bee n characterize d unde rth e GLP s an d th e stabilit yunde r storag e condition s at th e test sit e have no t bee n detennine d unde rGLPs
.

^ /



# 2=1 , 

# 7i \ Gar yHohenstein)Bponso rRepresentative

Pag e 3 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### QUALIT YASSURANC E STATEMEN T Repor t Title : Interi m Repor

t 0 9 Analysi s ofPFBS , PFHS , an d PFO S i n Groun d Wate rSample s Collecte d fro m Decatur , A L i n Septembe r201 0 Study : Analysi s ofPerfluorooctan e Sulfonate (PFOS) , Perfluorohexan e Sulfonat e (PFHS ) an d Perfluorobutan e Sulfonat e (PFBS ) i n Groundwater , Soi l an d Sedimen t fo rth e 3 M Decatu rPhas e 3 Site-Relate d Monitorin g Program
. Thi s analytica l phas e wa s audite d b yth e 3 M Environmenta l Laborator yQualit yAssurance Uni t (QAU) , as indicate d i n th e followin g table
. Th e finding s were reporte d t o th e principa l investigato r(P.I.) , laborator ymanagemen t an d stud ydirector
.
Inspectio n Date s 3/2/11-3/4/1 1 Phas e Dat a / Repor t Dat e Reporte d t o

Testin g Facilit y IVIanagemen t 3/21/1 1 Stud yDirecto r 3/21/1 1 

# C^>^V ^ / /(xr^-i^^eJZ/ i 

# I-^

- QA U Repre^ntativ e Date

Pag e 4 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0



### TABL E O F CONTENT S GL P Compliance statemen t 3 Qualit yAssuranc e Statemen t 4 Tabl e o fContents 5 List ofTable s 6 1 Stud yInformatio n 8 2 Summar

y9 3 Introductio n 1 0 4 Test s Contro l Substance s 1 1 5 Reference Substance s 1 2 6 Test Syste m 1 3 7 Metho d Summar

y1 3 7. 1 Method s 1 3 7. 2 Sampl e Collectio n 1 3 7. 3 Sampl e Preparatio n 1 3 7. 4 Analysi s 1 4 8 Analytica l Results 1 5 8. 1 Calibratio n 1 5 8. 2 Syste m Suitabilit y1 5 8. 3 Limi t ofQuantitatio n (LOQ ) 1 5 8. 4 Continuin g Calibratio n 1 6 8. 5 Blank

s 1 6 8. 6 La b Contro l Spike s (LCSs ) 1 6 8. 7 Analytica l Metho d Uncertaint y2 0 8. 9 Fiel d Matri xSpike s (FMS ) 2 1 9 Data Summar

yan d Discussio n 2 2

Pag e 5 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### 1 0 Conclusio n 33 1 1 Data/Sampl e Retentio n 33 1 2 Attachment s 33 1 3 Signature s 34 LIS TO F TABLE S Tabl e 1
. Summarize d PFBS , PFHS , an d PFO S Results (Decatu rGroundwater , Septembe r2010)
. .1 0 Sampl e Descriptio n Ke

yCod e 1 3 Tabl e 2
. Instrumen t Parameter s 1 4 Tabl e 3
. Liqui d Chromatograph yCondition s 1 4 Tabl e 4
. Mass Transition s 1 5 Tabl e 5
. Limi t ofQuantitatio n (LOQ ) 1 6 Tabl e 6
. Laborator

yContro l Spik

e Recover

y1 8 Tabl e 6 continued
. Laborator yContro l Spik

e Recover

y1 9 Tabl e 6 continued
. Laborator yContro l Spik

e Recover

y2 0 Tabl e 7
. Analytica l Uncertaint y2 1 Tabl e 8
. Fiel d Matri xSpike s 2 1 Tabl e 9
. DA LGW203 L10092 9 2 3 Tabl e 10
. DA LGW220 R 10093 0 2 3 Tabl e 11
. DA LGW220 L10093 0 2 4 Tabl e 12
. DA LGW222 R 10092 9 2 4 Tabl e 13
. DA LGW227 R 10092 4 2 5 Tabl e 14
. DA LGW227 L10092 4 2 5 Tabl e 15
. DA LGWGRS0 4 10093 0 2 6 Tabl e 16
. DA LG W3 1 O

R 10092 4 2 6 Tabl e 17
. DA LG W312 R 10093 0 2 7 Tabl e 18
. DA LG W317 L10092 4 2 7

Pag e 6 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### Tabl e 19
. DA L GW324 L 10092 9 2 8 Tabl e 20
. DA L G W327 R 10093 0 2 8 Tabl e 21
. DA L G W328 R 10092 8 2 9 Tabl e 22
. DA L GW328 L 10092 8 2 9 Tabl e 23
. DA L G W330 R 10092 3 3 0 Tabl e 24
. DA L GW330 L 10092 3 3 0 Tabl e 25
. DA L G W331 S 10093 0 3 1 Tabl e 26
. Tri p Blan k

1 3 1 Tabl e 26
. Tri p Blan k

2 32 Tabl e 27
. Rinseat e Blan k 32

Pag e 7 of8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### 1 Stud yInformatio n Sponso r 3 M Compan y Sponso rRepresentativ e Gar yHohenstei n 3 M EH S Operation s 3 M Buildin g 224-5W-0 3 Sain t Paul , M N 55144-100 0 Phone:(651)737-357 0 Stud yDirecto r Jaisimh a Kesari , P.E. , DE E Westo n Solutions , Inc
. Westchester , P

A1938 0 Phone:(610)701-376 1 Fax:(610)701-740 1 j.kesari@westonsolutions.co m Stud yLocatio n Testin g Facilit y 3 M EH S Operation s 3 M Environmenta l Laborator y Buildin g 260-5N-1 7 Maplewood , M N 5510 6 Stud yPersonne l Willia m K
. Reagen , Ph.D. , 3 M Laborator yManage r Clesto n Lange , Ph.D. , Principa l Analytica l Investigator , (clange(%mmm.com) : phon e (651)-733-986 0 Susa n Wolf , 3 M Analyst Chelsi e Grochow ; analys t Jonatha n Steege ; analys t Stud yDate s Stud yInitiation : Marc h 8 , 201 0 Interi m 0 9 Sampl e Collection : Septembe r2 3 - 30,201 0 Interi m 0 9 Experimenta l Termination : Marc h 16,201 1 Interi m Repor

t Completion : Dat e ofInteri m Repor t Signin g Locatio n o fArchive s Al l origina l rawdat a an d th e analytica l repor t have bee n archive d a

t th e 3 M Environmenta l Laborator yaccordin g t o 4 0 CF R Par t 792
. Th e tes t substanc e an d analytica l referenc e standard reserv e sample s ar e archive d at th e 3 M Environmenta l Laborator yaccordin g t o 4 0 CF R Par t 79 2

Pag e 8 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### 2 Summar y Th e 3 M Environmenta l Laborator yreceive d groundwate rsample s fro m well s locate d i n Decatur , AL , representin g 1 7 differen t samplin g locations
. Atota l o fseventy-seve n sampl e bottle s were receive d a t th e 3 M Environmenta l Laborator yfo rperfluorooctan e sulfonat e (PFOS) , perfluorohexan e sulfonate (PFHS ) an d perfluorobutan e sulfonat e (PFBS) , an d include d duplicat e groundwate rsample s fro m eac h samplin g location
. Sample s als o include d fiel d matri xspik e (FMS ) sample s fo reac h location , two tri p blank s containin g Milli-Q TM wate ran d appropriat e tri p blan k spikes , an d on e equipmen t rinseate blank
. Th e equipmen t rinseate blank s di d no t have FM S sample s prepare d fo rdeterminatio n o fPFBS , PFH S o rPFO S recovery . Al l groundwate rsample s fo rthi s projec t were logge d unde rGLP10-0 1 -02-09
. Th e groundwate rsamples , tri p an d equipmen t rinseate blank s were receive d fro m Westo n personne l o n Octobe r1 , 2010
. Al l ofth e sample s were prepare d an d analyze d fo rPFBS , PFHS , an d PFO S followin g 3 M Environmenta l Laborator yMetho d ETS-8-044. 0 an d conducte d unde r3 M projec t GLP-10 - 01-02-09
. Man yo fth e groundwate rsample s require d dilutio n t o attai n PFBS , PFHS , an d PFO S concentration s withi n th e rang e o fth e curve , i n som e instance s u p t o a 200-fol d dilutio n were required
. Th e averag e measure d PFBS , PFHS , an d PFO S concentration s are summarize d i n Tabl e 1
. Equipmen t rinseate an d th e tri p blank s were belo

wth e lowe rlimi t ofquantitatio n (LLOQ) , indicatin g adequat e contro l o fsampl e contaminatio n durin g shippin g an d sampl e collections
. Th e PFB S concentratio n results fo ral l groundwater s range d ove rfou rorder

s ofmagnitud e fro m 0.32 4 ng/m L t o 278 0 ng/mL
. Th e PFH S concentratio n results fo ral l groundwater s range d ove rfou rorder

s of magnitud e fro m 0.23 7 ng/m L t o 763 0 ng/mL
. Th e PFO S concentratio n results fo ral l groundwater s range d ove rfou rorder

s ofmagnitud e fro m <0.50 0 ng/m L t o 506 0 ng/mL
.

Pag e 9 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### Tabl e 1
. Summarize d PFBS , PFHS , an d PFO S Result s (Decatu rGroundwater , Septembe r 2010)
.
Samplin g Locatio n DA L GW203 L 10092 9 DA L GW220 R 10093 0 DA L GW220 L 10093 0 DA L GW222 R 10092 9 DA L GW227 R 10092 4 DA L GW227 L 10092 4 DA L GWGRS0 4 100930 DA L GW310 R 10092 4 DA L G W312 R 10093 0 DA L G W317 L 10092 4 DA L GW324 L 10092 9 DA L GW327 R 10093 0 DA L GW328 R 10092 8 DA L GW328 L 10092 8 DA L GW330 R 10092 3 DA L GW330 L 10092 3 DA L G W331 S 10093 0 Tri p Blan k

(Milli-Q TM Water

) DA L GW220 R R B 10093 0 (rinseat e blank ) PFB S Avg
. Cone
. (ng/mL ) ±RP D 70. 6 ±1. 4 10. 0 ±1. 8 7.8 5 ±3. 6 12 6 ±2. 4 16. 7 ±4. 2 37 6 ±0.5 3 241 0 ±3. 7 45 1 ±1. 3 142 0 ±0.7 1 0.32 4 '^' 89. 3 ±0.4 5 12 6 ±1. 6 31. 8 ±0.3 1 63. 1 ±3. 0 278 0 ±2. 2 63 6 ±5. 2 92 5 ±0.5 4 <0.25 0 <0.25 0 PFH S

Avg
. Cone
. (ng/mL ) ±RP D 48 1 ±6. 2 48. 7 ±0.6 2 41. 9 ±5. 7 87 2 ±0. 0 97. 5 ±1 1 25 5 ±0.3 9 763 0 ±4. 1 45 3 ±3. 1 84 2 ±1. 8 0.23 7 ±0.4 2 110±8. 2 22 9 ±1. 7 68. 0 ±5. 0 23. 6 ±8. 9 33 0 ±0.6 1 47 6 ±9. 2 *^

' 61 1 ±0. 0 <0.10 0 <0.10 0 PFO S

Avg
. Cone
. (ng/mL ) ±RP D 83 6 ±2. 8 70. 2 ±1 0 64. 6 ±4. 3 123 0 ±4. 9 85 2 ±4. 5 506 0 ±10'^ ' 137 0 ±8. 8 89 5 ±1 5 151 0 ±5. 3 <0.50 0 25 7 ±5. 8 67 5 ±0.7 4 16 8 ±3. 6 0.68 6 ±1 1 80 2 ±0.2 5 17 2 ±24'* ' 1180±1. 7 <0.99 9 <0.99 9 Th e analytica l metho d uncertaintie s associate d wit h th e reporte d results ar e a s follows : PFB S 100 % ±19% , PFH S 100 % ±26% , an d PFO S 100 % ±33%
. (1 ) Samplin g locatio n di d no t have a n appropriate FM S spik e leve l t o sufficientl yevaluate analyte recovery . (2) Asample/sampl e duplicat e RP D coul d no t b e calculate d since on e sampl e replicat e had a resul t ofBLOQ , (3) Th e analytica l uncertaint yfo rPFH S has bee n expande d t o ±35 % base d o n FM S recovery . (4) Th e RP D di d not meet metho d acceptance criteri a ofS20%
. 

## 3 Introductio n Thi s analytica l stud ywa s conducte d as par t ofth e Phas e 3 Environmenta l Monitorin g an d Assessmen t Progra m fo rth e 3 M facilitylocate d i n Decatur , Alabama
. Th e objective ofth e overal l progra m i s t o gai n informatio n regardin g concentration s ofperfluorooctan e sulfonat e (PFOS) , perfluorohexan e sulfonate (PFHS ) an d perfluorobutan e sulfonat e (PFBS) , i n variou s environmenta l medi a suc h as groundwater , soil s an d sediments that are associate d wit h an d nea rth e Decatu rfacility
. Thi s analytica l stud ywa s
Pag e 1 0 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S I n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### conducte d t o analyz e groun d wate rsample s collecte d fro m variou s well s locate d i n Decatur , A L fo r PFBS , PFHS , an d PFO S i n a n effor t t o characteriz e on-sit e groundwate rconditions
. Th e 3 M Environmenta l Laborator yprepare d sampl e container s (25 0 m L high-densit

ypolyethylen e bottles ) whic h were shippe d t o Decatur , A L Westo n personne l prio rt o fiel d sampling
. Sampl e container s fo reac h samplin g locatio n include d a fiel d sample , fiel d sampl e duplicate , an d fiel d spik e samples
. Eac h empt

ycontaine rwa s mari<e

d wit h a "fil l t o here " lin e t o produce a fina l sampl e volum e o f20 0 mL
. Container

s designate d fo rfiel d matri xsample s were fortifie d wit h a n appropriat e matri x spik e solutio n containin g PFB S (linear) , PFH S (linear) , an d PFO S (linear ) prio rt o bein g sen t t o th e fiel d fo rsampl e collection
. Al l sampl e bottle s include d th e additio n of^^Oa-PFBS , ^^Oa-PFHS , an d "Cg PFO S (intema l standards ) a t a nomina l concentratio n of1 ng/mL
. Al l sampl e bottle s als o include d th e additio n o f^'C4-PF0 S (sun^ogat e spike ) a t a nomina l concentratio n of0. 1 ng/mL
. Se e sectio n 8. 8 o f th e repor t fo rfiel d matri xspike levels
. Sample s were prepare d an d analyze d accordin g t o th e procedur e define d i n 3 M Environmenta l Laborator ymetho d ETS-8-044. 0 "Detenninatio n ofPerfluorinate d Compound s I n Wate rb yHig h Perforniance Liqui d Chromatography/Mas s Spectrometr yDirec t Injectio n Analysis"
. Tabl e 1 summarize s th e averag e PFBS , PFHS , an d PFO S concentration s fo rth e duplicat e samples collected , tri p blank

s an d equipmen t rinseate samples
. Table s 9-2 7 summariz e th e individua l sampl e results an d th e associate d FM S recoveries
. Al l results fo rth e qualit ycontro l sample s prepare d an d analyze d wit h th e sample s are reporte d an d discusse d elsewher e i n thi s repor

t 

## 4 Tes t & Contro l Substance s There wa s no

t a tes t substance o rcontro l substance s i n th e classi csens e ofa GL P study
. Thi s stud y wa s purel yanalytica l i n nature
.

Pag e 1 1 o f8 3

## 5 Referenc e Substance s GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0

Referenc e Substanc e Chemica l Nam e Chemica l Formul a Identifie r Sourc e Expiratio n Dat e Storag e Condition s Chemica l Lo t Numbe r TC R Numbe r Physica l Descriptio n Purit y PFB S (predominantl ylinear ) Perfluorobutan e sulfonat e C4F9S03 ' Potassiu m Sal t 3 M 1/10/201 7 Froze n 41-2600-8442- 5 TCR-12 1 Whit e Powde r 96.7 % PFH S (linear ) Perfluorohexan e sulfonat e C6F13SO 3 Sodiu m Sal t Wellingto n 4/2/201 3 Froze n LPFHxSAM0 8 TCR08-001 8 Crystallin e 98 %

Referenc e Substanc e Chemica l Nam e Chemica l Formul a Identifie r Sourc e Expiratio n Dat e Storag e Condition s Chemica l Lo t Numbe r TC R Numbe r Physica l Descriptio n Purit y PFO S (linear ) Perfluorooctan e sulfonat e CaFiySO s Potassiu m Sal t CA S #2795-39- 3 Wellingto n 10/18/201 3 Froze n LPFOSKBM0 6 TCR08-000 1 Crystallin e 98 % PFO S Perfluorooctan e sulfonat e C8F17SO3 " Potassiu m Sal t CA S # 2795-39- 3 3 M 12/14/201 6 Froze n 17 1 TCR-69 6 Whit e Powde r 86.4 %

Pag e 1 2 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



## 6 Tes t Syste m •• Th e tes t syste m fo rthi s stud yare groun d wate rsample s collecte d fro m well s locate d i n Decatur , A L b y Westo n Solutions , Inc
. personnel
. Sample s fo rthi s stud yare "rea l worid " samples , no t dose d wit h a specifi clot o ftest substance
. Sampl e Descriptio n Ke yCode
. Strin g Numbe r Exampl e 1 2 3 4
S Strin g Descripto r DA L GW203 L 010092 9 Sampl e Locatio n Sampl e Typ e Wel l I D Wel l Leve l Samplin g Dat e Sampl e Typ e Exampl e DA L = Decatur , A L GW = Groun d Wate r Example : 20 3 R = Residu m shallo wwater-bearin g zon e L = Bedroc k

water-bearin g zon e S = Epikars t middl e water-bearin g zon e 10092 9 = Septembe r29,201 0 0=primarysampl e 1=duplicat e sampl e L S = lo wspik e M S = mi d spik e H S = hig h spik e R B = rinseate blan k 

## 7 Mettio d Summar y 7. 1 Method s Analysi s fo ral l analyte s wa s complete d followin g 3 M Environmenta l Laborator ymetho d ETS-8-044. 0 "Detenninatio n ofPerfluorinate d Compound s I n Wate rb yHig h Perfomianc e Liqui d Chromatography/Mas s Spectrometr yDirec t Injectio n Analysis"
. 7. 2 Sampl e Collectio n Sample s were collecte d i n 25 0 m L Nalgene TM (high-densit ypolyethylene ) bottle s prepare d a t th e 3 M Environmenta l Laboratory
. Sampl e bottle s were retume d t o th e laborator ya t ambien t condition s o n Octobe r1 , 2010
. Sample s were store d refrigerate d a t th e laborator

yafte rreceipt
. Aset oflaborator y prepare d Tri p Blan k

an d Tri p Blan k

fiel d matri xspike s were sen t wit h th e sampl e collectio n bottles
. 7. 3 Sampl e Preparatio n Sample s were prepare d byremovin g a n aliquot o fth e wel l mixe d sampl e an d placin g it i n a n autovia l fo r analysis
. Sample s that require d dilutio n were prepare d as follows : 1:1 0 dilution s were prepare d b ydilutin g 1 m L sampl e wit h 9 m L oflaborator yreagen t water , 1:20 dilution s were prepare d b ydilutin g 0.5m L sampl e wit h 9.5m L oflaborator yreagen t water , 1:50 dilution s were prepare d b ydilutin g 0.2m L sampl e wit h 9.8m L o flaborator yreagen t water , 1:100 dilution s were prepare d b ydilutin g 0. 1 m L sampl e wit h 9.9m L oflaborator yreagen t water , an d 1:500 dilution s were prepare d b yremovin g 2m L ofth e 1:100 dilution s an d furthe rdilutin g i t wit h 8m L of laborator yreagen t water
.

Pag e 1 3 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### Sample s ma

yals o have bee n dilute d furtite rb yvaryin g th e injectio n volum e durin g analysis
. 7. 4 Analysi s Al l stud ysample s an d qualit ycontro l sample s were analyze d fo rPFBS , PFHS , an d PFO S usin g hig h performance liqui d chromatography/tande m mass spectrometr y(HPLC/MS/MS)
. Detaile d instrumen t parameters , th e liqui d chromatograph ygradien t program , an d th e specifi cmass transition s analyze d ar e describe d i n th e ra

wdat a har

d copie s place d i n th e fina l data packet , an d are briefl ydescribe d below
. Tabl e 2
. Instrumen t Parameters
. Instrumen t Nam e Analytica l Metho d Followe d Analysi s Dat e Liqui d Chromatograp h Guard colum n Analytica l colum n Injectio n Volum e Mas s Spectromete r Io n Sourc e Electrod e Polarit y Softwar e ETS Buste r ETS-8-044. 0 10/25/10,11/8/10 , an d 3/15/1 1 Agilen t 1100 10/25/10 ; Betasl l 01 8 (2. 1 m m X 100 mm) , 5 n 11/8/10 ; Pris m R P (2. 1 m m X 50 mm) , 5 u Betasl l C1 8 (2. 1 m m X 100 mm) , 5 n

5,2 , o r1 n L Applie d Biosystem s AP I 400 0 Turtr o Spra y Turtx) io n electrod e Negativ e Analyst 1.4. 2 ETS Ginge r ETS-8-044. 0 2/11/1 1 Agilen t 1100 Pris m R P (2. 1 m m X 5 0 mm) , 5 n Betasl l 01 8 (2. 1 m m X 100 mm) , 5 n

2or10M L Applie d Biosystem s AP I 500 0 TurtD O Spra y Turb o io n electrod e Negative Analyst 1.4. 2 

### Tabl e 3
. Liqui d Chromatograph yConditions
. ste p Numbe r Tota l Tim e

(min ) Flo wRat e

(/jL/min ) Percen t A

( 2 m M ammoniu m acetate ) Percent B (Methanol ) ETS-8-044. 0 Analysi s 10/2511 0 an d 11/8/1 0 0 1 2 3 4 5 0

2. 0 14. 5 15. 5 16. 5 20. 0 30 0

30 0 30 0 30 0 30 0 30 0 97. 0 97. 0 5. 0 5. 0 97. 0 97. 0 3. 0 3. 0 95. 0 95. 0 3. 0 3. 0 ETS-8-044.0/Analysi s 2/11/1 1 and3/1S/1 1 0 1 2 3 4 5 0

2. 0 11. 0 14. 0 14. 5 17. 0 30 0

30 0 30 0 30 0 30 0 30 0 97. 0 97. 0 5. 0 5. 0 97. 0 97. 0 3. 0 3. 0 95. 0 95. 0 3, 0 3. 0

Pag e 1 4 o f8 3

### Tabl e 4
. Mas s Transitions
. GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0

Analyt e
PFB S

PFH S
PFO S Referenc e Materia l

Structur e Linea r
Linea r
Linea r Mas s Transitio n

Q1/Q 3 299/8 0 299/9 9 299/13 0 399/8 0 399/9 9 399/13 0 499/8 0 499/9 9 499/13 0 Dwel l tim e was 5 0 mse c(2/11/1 1 an d 3/15/1 1 analysis ) o r75 mse c (10/25/10an d 11/8/1 0 analysis ) fo reac h transition
. Th e individua l transition s were summe d t o produce a "tota l Io n chromatogram " (TIC) ,

whic h was use d fo rquantitation
. 

### 8 Analytica l Result s 8. 1 Calibratio n Sample s were analyze d against a n externa l standar d calibratio n curve . Calibratio n standard s were prepare d b

yspikin g know n amounts ofth e stoc k solutio n containin g th e targe t analyte s into prepare d i n Mill i Q water
. Atota l offiftee n spike d standard s rangin g fro m 0.02 5 ng/m L t o 10 0 ng/m L (nominal ) were prepared , however , no

t al l analytica l run s include d al l fiftee n standards
. Lowcurve points were disable d t o meet accurac

yo rmetho d blan k

criteria
. Aquadratic , 1/

xweighted , calibratio n curve ofth e pea k are a counts wa s use d t o fi t th e dat a fo reac h analyte
. Th e dat a were no t force d throug h zero durin g th e fittin g process . Calculatin g th e standar d concentration s usin g th e pea k are a confimned accurac

yofeac h curve point
. Eac h curve poin t wa s quantitate d usin g th e overal l calibratio n curve an d reviewe d fo raccuracy . Method calibratio n accurac

yrequirements of100±25 % (100±30 % fo rth e lowest curve point) were me t fo ral l analytes
. Th e con-elatio n coefficien t (r ) wa s greate rtha n 0.99 5 fo rPFBS , PFHS , an d PFO S fo reac h analytica l batch
. Du e t o th e concentratio n ofPFBS , PFHS , an d PFO S detecte d i n th e samples , isotopically-labele d PFBS , PFHS , an d PFO S were no t use d durin g instrumen t calibration
. I n addition , du e t o th e numbe rof sample s requirin g dilution , th e "C4-PFO S sun-ogat e was no t analyzed
. 

## 8. 2 Syste m Suitabilit y Acalibratio n standar

d wa s analyze d fou rtime s a t th e beginnin g ofth e analytica l sequence t o demonstrate overal l syste m suitability
. Th e acceptance criteri a ofless tha n o requa l t o 5% relative standar d deviatio n (RSD ) fo rpea k are a an d retentio n tim e criteri a o fles s tha n o requa l t o 2 % RS D wa s met fo rPFBS , PFHS , an d PFO S fo reac h analytica l batch
. 8. 3 Limi t o fQuantitatio n (LOQ ) Th e LOQ fo rthi s analysi s i s th e lowest non-zer o calibratio n standar

d i n th e curve that meets linearit y an d accurac

yrequirements an d fo rwhic h th e are a counts ar e a t least twice thos e ofth e appropriat e blanks
. Th e LOQs associate d wit h th e sampl e analysi s are liste d i n th e tabl e below
.

Pag e 1 5 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0



### Tabl e 5
. Limi t o fQuantitatio n (LOQ)
. Analysi s Dat e 10/25/1 0

11/8/1 0 2/11/1 1 3/15/1 1 Dilutio n

1 1 0 2 0 5 0 100 1 5 0 100 50 0 1 PFB S

LOQ , ng/m L 0.25 0 2.5 0 5.00 12. 5 25. 0 0.25 0 N A 2.5 0 12. 5 N A PFH S

LOQ , ng/m L 0.10 0 1.0 0 2.0 0 5.00 10. 0 0.10 0 N A NA 12. 5 NA PFO S

LOQ , ng/m L 0.99 9 9.9 9 20. 0 50. 0 99. 9 0.50 0 25. 0 N A 15. 0 0.050 0 NA= No t /Applicable ; analytica l results at th e spedfie d dilutio n wer e no t reporte d fro m th e run . 

### 8. 4 Continuin g Calibratio n Durin g th e cours e ofeac h analytica l sequence , continuin g calibratio n verificatio n sample s (CCVs) were analyze d t o confin n that th e instrumen t respons e an d th e initia l calibratio n curve were stil l i n control
. Al l CCV s met metho d criteri a of100 % ±25 % wit h th e followin g exceptions : 10/25/1 0 Analysis : Founrtee n CCVs were anayze d durin g th e cours e o fth e oin
. Two CCVs fo rPFB S were outsid e metho d acceptance crteria
. On e CCV fo rPFB S had a recover yof137 % an d brackete d sample s DA L G W310R , DA L G W312R , DA L G W317L , an d DA L G W324L
. Asecon d CCV fo rPFB S had a recover

y130 % an d brackete d sample s DA L G W330L , DA I G W331S , an d th e Tri p Blan k samples
. Two CCVs fo rPFO S had recoverie s of128 % an d 127 % an d brackete d sample s DA L G W 222R , DA L G W227L , an d DA L G WGRS04
. 

## 8. 5 Blank s Thre e type s o fblank

s were prepare d an d analyze d wit h th e samples : metho d blanks , field/tri p blanks , an d equipmen t blanks
. Metho d blan k results were reviewe d an d use d t o evaluat e metho d perfonnance an d t o determin e th e LOQ fo reac h analyt e 8. 6 La b Contro l Spike s (LCSs ) Aminuimu m o ftw o la b contro l spike level s were prepare d an d analyze d i n triplicat e wit h eac h preparatio n set
. LCS s were prepare d b yspikin g know n amounts o fth e analyt e into 1 0 m L ofMill i Q wate rt o produce th e desire d concentration
. Th e spike d wate rsample s were the n analyze d i n th e sam e manne ras th e samples
. Th e metho d acceptance criteria , averag e o fLC S a t eac h leve l shoul d b e withi n 100 % ±20 % wit h a n RS D <20%
. 10/25/1 0 Analysis : Two level s o fLC S were prepare d an d me t metho d acceptance criteri a fo rPFBS , PFHS , an d linea rPFOS
. 11/8/1 0 Analysis : Two level s ofLC S were prepare d an d me t metho d acceptance criteri a fo rPFBS , PFHS , an d linea rPFOS
. 2/11/1 1 Analysis : Thre e level s ofLC S were prepare d an d me t metho d acceptance crtieri a fo rPFBS , PFHS , an d PFOS
. 3/15/1 1 Analysis : Two level s ofLC S were prepare d an d me t metho d acceptance criteri a fo rPFOS
.

Pag e 1 6 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### A s th e reference material s use d fo rquantitatio n ofPFO S i s predominantl ylinear , an d th e PFO S presen t i n th e wate rsample s are comprise d ofbot h linea ran d branche d isomers , additiona l LC S sample s of PFO S (linea r+ branched ) were prepare d t o evaluat e th e potentia l fo ranalytica l bias
. 10/25/1 0 Analysis : Two level s oflinea ran d branche d PFO S LC S were prepare d an d met metho d acceptance criteria
. 11/8/1 0 Analysis : Two level s oflinea ran d branche d PFO S LC S were prepare d wit h th e hig h set ofLC S havin g a n averag e recover yof129%
. 2/11/1 1 Analysis : Thre e level s oflinea ran d branche d PFO S LC S were prepare d wit h th e hig h set of LC S havin g a n averag e recover yo f133%
. 3/15/1 1 Analysis : Two level s oflinea ran d branche d PFO S LC S were prepare d an d me t metho d acceptance criteria
. Th e averag e recover

yo fth e hig h set o flinea ran d branche d PFO S LC S a t 129 % an d 133 % suggests a sligh t bias whe n quantitatin g branche d isomer s o fPFO S against a calibratio n curve oflinea rPFOS
. Therefore , th e metho d uncertaint yfo rthi s stud yhas bee n set o f±33% , base d o n th e highest averag e recover yo fth e linea ran d branche d LC S samples
. Ametho d deviatio n i s file d wit h th e ra wdat a fo rPFO S (linea r+ branched ) LC S that di d no t mee t method acceptance criteria
. Th e followin g calculation s were use d t o generat e dat a i n Tabl e 6 fo rlaborator ycontro l spikes
. I o o r , . n , Calculate d Concentratio n . . „„„ , LC S Percen t Recover

y= * 100 % Spike Concentratio n LCS % RS D = Standar

d deviatio n LC S replicate s . ^ ^^y ^ averag e LC S recover

y

Pag e 1 7 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### Tabl e 6
. Laborator yContro l Spik e Recovery
. ETS-8-044. 0 Analyze d 10/25/1 0
La b I D LCS-101020- 1 LCS-101020- 2 LCS-101020- 3 Averag e ±%RS D LCS-101020- 4 LCS-101020- 5 LCS-101020- 6 Averag e ±%RS D PFB S Spike d Concentratio n (ng/mL ) 2.0 0 2.0 0 2.0 0 Calculate d

Concentratio n (ng/mL ) 1.86 1.98 1.98 %Recover y 93. 2 98. 9 99. 1 97.1 % ±3.5 % 20. 0 20. 0 20. 0 17. 4

19. 6 19. 1 86. 8

97. 8 95. 5 93.4 % ±6.2 % PFH S Spike d Concentratio n 2.0 0 2.0 0 2.0 0 Calculate d

Concentratio n (ng/mL ) 1.89 1.98 1.97 %Recover y 94. 6 99. 2 98. 4 97.4 % ±2.5 % 20. 0 20. 0 20. 0 17. 3

19. 5 18. 7 86. 4

97. 6 93. 5 92.5 % ±6.1 %
ETS-8-044. 0 Analyze d 10/25/1 0
La b I D LCS-101020- 1 LCS-101020- 2 LCS-101020- 3 Averag e ±%RS D LCS-101020- 4 LCS-101020- 5 LCS-101020- 6 Averag e ±%RS D PFO S (linear) Spike d Concentratio n (ng/mL ) 2.0 0 2,0 0 2.0 0 Calculate d

Concentratio n (ng/mL ) 1.70 1.79 1.79 VoRecover y 85. 2 89. 7 89. 5 88.1 % ±2.9 % 20. 0 20. 0 20. 0 17. 0

19. 3 18. 6 84. 9

96, 5 92. 8 91.4 % ±6.5 % La b I D LCS-101020- 7 LCS-101020- 8 LCS-101020- 9 Averag e ±%RS D LCS-101020-1 0 LCS-101020-1 1 LCS-101020-1 2 Averag e ±%RS D PFO S (linear+branched ) Spike d Concentratio n (ng/mL ) 1,98 1,98 1,98 Calculate d

Concentratio n (ng/mL ) 1,9 1 1.89 1,9 1 %Recover

y 96, 7 95, 6 96, 4 96.2 % ±0.59 % 19. 8 19, 8 19, 8 21, 5

21, 5 21, 6 10 8

10 8 10 9 108 % ±0.53 % ETS-8-044, 0 Analyze d 11/8/1 0
La b I D LCS-101105- 1 LCS-101105- 2 LCS-101105- 3 Averag e ±%RS D LCS-101105- 4 LCS-101105- 5 LCS-101105- 6 Averag e ±%RS D PFB S Spike d Concentratio n (ng/mL ) 2,0 0 2,0 0 2,0 0 Calculate d

Concentratio n (ng/mL ) 2,4 4 2.3 6 2.2 9 VoRecover y 12 2 11 8 11 5 118 % ±3.0 % 20, 0 20. 0 20. 0 24, 3

23, 8 22, 5 12 1

11 9 11 3 118 % ±3.5 % PFH S Spike d Concentratio n (ng/mL ) 2,0 0 2,0 0 2,0 0 Calculate d

Concentratio n (ng/mL ) 2,4 7 2,3 7 2,2 8 VoRecover y 12 4 11 8 11 4 119 % ±4.2 % 20. 0 20, 0 20. 0 24,0 0

24.0 0 23,0 0 12 0

12 0 11 5 118 % ±2.4 % (1 ) Th e averag e recover ydi d no t meet metho d acceptance criteri a of100% ±20% ,

Pag e 1 8 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0



### Tabl e 6 continued
. Laborator yContro l Spik e Recovery
. ETS-8-044. 0 Analyze d 11/8/1 0
La b I D LCS-101105- 1 LCS-101105- 2 LCS-101105- 3 Averag e ±%RS D LCS-101105- 4 LCS-101105- 5 LCS-101105- 6 Averag e ±%RS D Spike d

Concentratio n (ng/mL ) 2,0 0 2,0 0 2,0 0 PFO S (lineal )

Calculate d Concentratio n (ng/mL ) 2,3 0 2,2 3 2,1 7 VoRecover y 11 5 11 1 10 8 111 % ±3.2 % 20, 0 20, 0 20. 0 23, 7

24, 6 23. 1 11 9

12 3 11 5 119 % ±3.4 % La b I D LCS-101105- 7 LCS-101105- 8 LCS-101105- 9 Averag e ±%RS D LCS-101105-1 0 LCS-101105-1 1 LCS-101105-1 2 Averag e ±%RS D PFO S (linear+branched ) Spike d Concentratio n (ng/mL ) 1.98 1,98 1,98 Calculate d

Concentratio n (ng/mL ) 2,1 4 2,5 8 2,1 8 VoRecover

y 10 8 13 0 11 0 116 % ±10 % 19, 8 19, 8 19, 8 24, 3

27, 2 24. 9 12 3

13 8 12 6 129 % ±6.2%'' ' ETS-8-044, 0 Analyze d 2/11/1 1
La b I D LCS-110210- 1 LCS-110210- 2 LCS-110210- 3 Averag e ±%RS D LCS-110210- 4 LCS-110210- 5 LCS-110210- 6 Averag e ±%RS D LCS-110210- 7 LCS-110210- 8 LCS-110210- 9 Averag e ±%RS D Spike d

Concentratio n (ng/mL ) 0,49 8 0,49 8 0,49 8 PFB S

Calculate d Concentratio n (ng/mL ) 0,56 3 0,54 2 0,58 1 VoRecover y 11 3 10 9 11 7 113 % ±3.5 % 4.9 8 4,9 8 4.9 8 5,3 7

5,5 2 5,5 8 10 8

11 1 11 2 110 % ±1.9 % 29, 9 29, 9 29, 9 29, 8

31, 1 32, 0 99, 5

104 10 7 104 % ±3.6 % Spike d Concentratio n (ng/mL ) 0,49 8 0,49 8 0,49 8 PFH S

Calculate d Concentratio n (ng/mL ) 0,60 3 0,59 4 0,59 9 %Recovei y

12 1 11 9 12 0 120 % ±0.83 % 4,9 8 4,9 8 4,9 8 5.6 1

5,3 6 5,9 6 11 3

108 12 0 114 % ±5.3 % 29, 9 29, 9 29, 9 30, 7

30, 8 32, 7 10 3

10 3 10 9 105 % ±3.3 % (1 ) Th e averag e recover ydi d no t meet metho d acceptance criteri a of100 % ±20% ,

Pag e 1 9 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S I n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0



### Tabl e 6 continued
. Laborator yContro l Spik e Recovery
. ETS-8-044, 0 Analyze d 2/11/1 1
La b I D -CS-110210- 1 .CS-110210- 2 LCS-110210- 3 Averag e ±%RS D LCS-110210- 4 LCS-110210- 5 LCS-110210- 6 Averag e ±%RS D LCS-110210- 7 LCS-110210- 8 LCS-110210- 9 Averag e ±%RS D Spike d

Concentratio n (ng/mL ) 0,49 8 0,49 8 0,49 8 PFO S (lineal )

Calculate d Concentratio n (ng/mL ) 0.65 0 0,58 7 0,54 0 VoRecover y 13 0 11 8 10 8 119 % ±9.3 % 4.9 8 4.9 8 4,9 8 5,4 3

4.9 3 5,8 6 10 9

99, 1 11 8 109 % ±8.7 % 29. 9 29. 9 29. 9 30. 8

30, 7 32, 4 10 3

10 3 10 8 105 % ±2.8 % La b I D LCS-110210-1 0 LCS-110210-1 1 LCS-110210-1 2 Averag e ±%RS D LCS-110210-1 3 LCS-110210-1 4 LCS-110210-1 5 Averag e ±%RS D LCS-110210-1 6 LCS-110210-1 7 LCS-110210-1 8 Averag e ±%RS D PFO S Spike d Concentratio n (ng/mL ) 0,50 2 0,50 2 0,50 2 ' (linea r•*

•branched ) Calculate d Concentratio n (ng/mL ) 0.53 9 0.58 5 0.54 1 VoRecover

y 107 11 7 10 8 111 % ±5.0 % 5,0 4 5,0 4 5,0 4 6.1 5

5.3 8 5.4 4 122

10 7 10 8 112 % ±7.5 % 30, 2 30, 2 30, 2 40, 7

40. 4 39, 6 135

134 13 1 133 % ±1.6%' " ETS-8-044, 0 Analyze d 3/15/1 1
La b I D LCS-110315- 1 LCS-110315- 2 .CS-110315- 3 Averag e ±%RS D LCS-110315- 4 LCS-110315- 5 LCS-110315- 6 Averag e ±%RS D Spike d

Concentratio n (ng/mL ) 0.49 8 0.49 8 0.49 8 PFO S (linear)

Calculate d Concentratio n (ng/mL ) 0,45 6 0,49 7 0,46 3 VoRecover y 91, 5 99, 9 93. 0 94.8 % ±4.7 % 4.9 8 4,9 8 4,9 8 4,3 5

5,0 5 5,0 4 87. 3

10 1 10 1 96.4 % ±8.2 % La b I D LCS-110315- 7 LCS-110315- 8 LCS-110315- 9 Averag e ±%RS D LCS-110315-1 0 LCS-110315-1 1 LCS-110315-1 2 Averag e ±%RS D PFOS Spike d Concentratio n (ng/mL ) 0,50 2 0,50 2 0,50 2 (linear * branched )

Calculate d Concentratio n (ng/mL ) 0,42 7 0,45 6 0,42 9 VoRecover

y 85, 1 90, 9 85, 5 87.2 % ±3.7 % 5,0 4 5,0 4 5.0 4 5,0 2

4,9 0 5,1 2 99, 6

97. 2 102 99.6 % ±2.4 % (1 ) Th e averag e recover ydi d no t meet metho d acceptance criteri a of100 % ±20% , 

## 8. 7 Analytica l IVIetho d Uncertaint y Analytica l uncertaint

yi s base d o n historica l Q C data that i s contro l charte d an d use d t o evaluat e metho d accurac

yan d precision
. Th e metho d uncertaint yi s calculate d followin g ETS-12-012,2 , Th e standar d deviatio n i s calculate d fo rth e set ofaccurac yresults (i n % ) obtaine d fo rth e Q C samples
. Th e expande d uncertaint

yi s calculate d b

ymultiplyin g th e standar

d deviatio n b

ya facto rof2 , whic h con-espond s t o a confidence leve l o f95%
. Th e mos t recen t 5 0 dat a points were use d t o generate th e metho d uncertaint

yvalues
. Th e metho d uncertaint yfo rPFO S b

yETS-12-012- 2 wa s foun d t o b e ± 27 % however , since on e o fth e hig h sets o flinea ran d branche d LC S fo rPFO S had a n averag e recover yof133% , th e analytica l uncertaint yassigne d t o thi s stud yfo rPFO S wil l b e ±33%
.

Pag e 2 0 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### Tabl e 7
. Analytica l Uncertainty
. Analyt e PFB S PFH S PFO S standar d Deviatio n

9.52 13. 2 N A Metho d Uncertainty

±19 % ±26 % ±33%" ' (1 ) Analytica l uncertaint yfo rPFO S was base d o n batc h QC prepare d wit h th e stud ysamples
. 

### 8. 8 Fiel d Matri xSpike s (FMS ) Fiel d matri xspike s were collecte d a t eac h samplin g poin t t o verif ythat th e analytica l metho d i s applicabl e t o th e collecte d matrix
. Fiel d matri xspike s were generate d b yaddin g a measure d volum e of fiel d sampl e t o a containe rspike d b yth e laborator

ywit h PFB S (linear) , PFH S (linear) , an d PFO S (linear ) prio rt o shippin g sampl e container s fo rsampl e collection
. Fiel d matri xspik e recoverie s withi n metho d acceptance criteri a o f100±30 % confin n that "unknown " components i n th e sampl e matri xd o no t significantl yinterfere wit h th e extractio n an d analysi s o fth e analyte s ofinterest
. Fiel d matri xspik e concentration s must b e 50 % ofth e sampl e concentratio n t o b e considere d a n appropriat e fiel d spike
. Fiel d matri xspike s are presente d i n sectio n 9 o fthi s report
. Tabl e 8
. Fiel d Matri xSpikes
. Samplin g Locatio n 317 L
220R , 220L , 328 L '^' 203L , 222R , 227L , 227R , 310R , 324L , 327R , 330L'^331 S GRS04 , 312R , 330 R 328R" '
Tri p Blan k

Set s 1- 4 Spik e Leve l Lo wFM S Hig h FM S Lo wFM S Hig h FM S LowFM S Hig h FM S Lo wFM S Hig h FM S Lo wFM S Hig h FM S Lo wFM S Mi d FM S Hig h FM S PFB S

(ng/mL ) 1,00 10, 0 10, 0 10 0 10 0 100 0 100 0 500 0 10 0 50 0 10. 0 10 0 100 0 PFH S

(ng/mL ) 1,00 10. 0 10, 0 10 0 10 0 100 0 100 0 500 0 10 0 50 0 10. 0 10 0 100 0 PFO S

(ng/mL ) 1.00 10, 0 10, 0 10 0 10 0 100 0 100 0 500 0 10 0 50 0 10, 0 10 0 100 0 (1 ) Sampl e bottle s were overtille d b ymor e tha n 10 % fo rDA L GW328 R H S (22 1 mL) , DA L G W328 L L S (22 4 mL) , DA L G W330 L L S (22 1 mL) , an d DA L G W330 L H S (22 4 mL) , Th e FM S spik e concentratio n has bee n adjuste d accordingly
. 

### Du e t o th e hig h concentratio n o fPFBS , PFHS , an d PFO S detecte d i n th e samples , th e sun-ogat e spik e of^^C4-PF0 S wa s not analyzed
.

(Sampl e Concentratio no f FMS-Averag e Concentration : Fiel dSampl e & Fiel dSampl e Dup. ) FM S Recovery = .^

^ J-^ ' Spik e Concentrato n 100 %


### 8. 9 La b Matri xSpike s (LMS ) Samplin g locatio n DA L G WGRS04 10093 0 hig h spik e a t 500 0 ng/m L produce d recoverie s <50 % whe n initiall yananyze d o n 10/25/10
. Alaborator ymatri xspik

e a t 500 0 ng/m L wa s prepare d an d analyze d o n 2/11/11
. Th e la b matri xspike resul t i s presente d i n sectio n 9 ofthi s report
.

Pag e 2 1 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



## \ 9 : Dat a Summar yan d Discussio n Th e table s belowsummariz e th e sampl e results an d fiel d matri xspike recoverie s fo rth e samplin g location s as wel l as th e Tri p Blank s an d rinseate blank
. Results an d averag e value s are rounde d t o thre e significan t figure s accordin g t o EP Aroundin g njles
. Becaus e ofrounding , value s ma yvar yslightl y fro m thos e liste d i n th e ra wdata
. Fiel d matri xspike recoverie s meetin g th e metho d acceptance criteri a of±30% , demonstrat e that th e metho d wa s appropriat e fo rth e give n matri xan d thei rrespective quantitative ranges . DA L G W330L10092 3 - Th e recover yofth e hig h FM S sampl e fo rPFH S wa s 135%
. Thi s wa s th e onl yappropriat e fiel d matri xspik e leve l fo rPFHS
. Th e metho d uncertaint yhas bee n adjuste d fo r PFH S t o ±35%
. DA L G WGRS0410092 3 -Th e recover yofth e hig h FM S sampl e fo rPFBS , PFHS , an d PFO S were <50%
. ALM S a

t 5000pp b wa s prepare d o n th e sampl e wit h acceptabl e recoverie s fo rPFBS , PFHS , an d PFOS
. DA L G W328L10092 8 - Th e recover yofth e hig h FM S sampl e fo rPFO S wa s 69.0% , whil e th e recover yofth e lo wFM S wa s 102%
. Since th e lo wFM S wa s th e mor

e appropriat e spike leve l as compare d t o th e sampl e concentration , n o adjustmen t wa s mad e t o th e analytica l uncertainty
.

Pag e 2 2 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### Tables
. DA L GW203 L 10092 9

3 M LIM S I D GLP10-01-02-09-00 1 GLP10-01-02-09-00 2 GLP10-01-02-09-00 3 GLP10-01-02-09-00 4 Descriptio n DA L GW203 L 0 10092 9 DA L GW203LD B 10092 9 DA L GW203LL S 10092 9 DA L GW203LH S 10092 9 Averag e Concentratio n (ng/mL ) ±%RP D PFB S Concentratio n (ng/mL ) 70, 1 71. 1 175 100 0 VoRecover

y N A N A 10 4 92. 9 70. 6 ng/m L ±1.4 % PFH S Concentratio n (ng/mL ) 46 6 49 6 64 3 170 0 %Recover

y N A N A N C 122 48 1 ng/m L ±6.2 % PFO S Concentratio n (ng/mL ) 82 4 84 7 90 7 193 0 VoRecover

y N A NA N C 10 9 83 6 ng/m L ±2.8 % NA= No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2xspik e level
. Samples dilute d 20

xan d analyze d 10/25/1 0 an d 11/8/10
. 

### Tabl e 10
. DA L GW220 R 10093 0

3MUMSI D GLP10-01-02-09-00 5 GLP10-01-02-09-00 6 GLP10-01-02-09-00 7 GLP10-01-02-09-00 8 Descriptio n DA L GW220 R 010093 0 DA L GW220 R D B 100930 DA L GW220RL S 100930 DA L GW220RH S 100930 Averag e Concentratio n (ng/mL ) ±%RP D PFB S Concentratio n (ng/mL ) 9.9 2 10. 1 20. 1 11 2 VoRecover

y N A N A 10 1 10 2 10. 0 ng/m L ±1.8 % PFH S Concentratio n (ng/mL ) 48, 5 48. 8 59. 3 15 0 VoRecover

y N A N A N C 10 1 48. 7 ng/m L ±0.62 % PFO S Concentratio n (ng/mL ) 73. 8 66. 5 81. 1 152 VoRecover

y N A N A N C 81. 9 70. 2 ng/m L ±10 % NA= No t Applicatd e N C = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2xspik e level
. Samples dilute d lOxan d analyze d 10/25/10
.

Pag e 2 3 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### Tabl e 11
. DA L G W220 L 10093 0

3IVIUMSI D GLP10-01-02-09-00 9 GLP10-01-02-09-01 0 GLP10-01-02-09-01 1 GLP10-01-02-09-01 2 Descriptio n DA L GW220 L 0 10093 0 DA L GW220LD B 10093 0 DA L GW220LL S 10093 0 DA L GW220LH S 10093 0 Averag e Concentratio n (ng/mL ) ±%RP D PFB S Concentratio n (ng/mL ) 7.9 9 7.7 1 20. 8 11 2 VoRecover

y N A N A 13 0 104 7.8 5 ng/m L ±3.6 % PFH S Concerrtratio n (ng/mL ) 40. 7 43. 1 55. 4 15 0 VoRecover

y N A N A N C 10 8 41. 9 ng/m L ±5.7 % PFO S Concentratio n (ng/mL ) 63. 2 66. 0 80. 8 152 VoRecover

y N A N A N C 87. 4 64. 6 ng/m L ±4.3 % NA = No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2xspik e level
. Sample s dilute d 20

xan d analyze d 10/25/10
. 

### Tabl e 12
. DA L GW222 R 10092 9

3IVILIIVISI D GLP10-01-02-09-01 3 GLP10-01-02-09-01 4 GLP10-01-02-09-01 5 GLP10-01-02-09-01 6 Descriptio n DA L GW222 R 010092 9 DA L GW222RD B 10092 9 DA L GW222RL S 10092 9 DA L GW222 R H S 10092 9 Averag e Concentratio n (ng/mL ) ±%RP D PFB S Concentratio n (ng/mL ) 12 4 12 7 23 8 120 0 VoRecover

y N A N A 11 3 107 12 6 ng/m L ±2.4 % PFH S Concentratio n (ng/mL ) 87 2 87 2 99 6 201 0 VoRecover

y N A N A N C 11 4 87 2 ng/m L ±0.0 % PFO S Concentratio n (ng/mL ) 120 0 126 0 122 0 231 0 VoRecover

y N A N A N C 10 8 123 0 ng/m L ±4.9 % NA= No t /Applicabl e N C = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2xspik e level
. Sample s dilute d 50

xan d analyze d 10/25/10
.

Pag e 2 4 of8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0



### Tabl e 13
. DA L GW227 R 10092 4

3MUMSI D GLP10-01-02-09-01 7 GLP10-01-02-09-01 8 GLP10-01-02-09-01 9 GLP10-01-02-09-02 0 Descriptio n DA L GW227 R 010092 4 DA L GW227RD B 10092 4 DA L GW227RL S 10092 4 DA L GW227RH S 10092 4 Averag e Concentratio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 16. 3 17. 0 11 7 103 0 VoRecover

y N A N A 10 0 10 1 16. 7 ng/m L ±4.2 % PFH S Concentratio n (ng/mL ) 10 3 91. 9 18 5 119 0 VoRecover

y N A N A 87. 6 109 97. 5 ng/m L ±11 % PFO S Concentratio n (ng/mL ) 87 1 83 3 80 1 205 0 VoRecover

y N A N A N C 12 0 85 2 ng/m L ±4.5 % N A= No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2xspik e level
. Sample s dilute d 50

xan d analyze d 10/25/10
. 

### Tabl e 14
. DA L GW227 L 10092 4

3 M LIM S I D GLP10-01-02-09-02 1 GLP10-01-02-09-02 2 GLP10-01-02-09-02 3 GLP10-01-02-09-02 4 Descriptio n DA L GW227 L 010092 4 DA L GW227LD B 100924 DA L GW227LL S 100924 DA L GW227LH S 100924 Averag e Concentratio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mt j 37 7 37 5 47 4 127 0 VoRecover

y N A N A N C 89. 4 37 6 ng/m L ±0.53 % PFH S Concentratio n (ng/mL ) 25 4 25 5 36 4 126 0 VoRecover

y N A N A N C 10 1 25 5 ng/m L ±0.39 % PFO S Concentratio n (ng/mL ) 479 0 532 0 568 0 686 0 %Recover

y N A N A N C N C 506 0 ng/m L ±10%" ' N A= No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2 xspike level
. Sample s dilute d 20

xfo rPFB S an d PFHS , an d lOO xfo rPFO S an d analyze d o n 10/25/10
. (1 ) Samplin g locatio n di d no t hav e a n appropriate field matri xspik e leve l fo rPFOS
.

Pag e 2 5 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0



### Tabl e 15
. DA L GWGRS0 4 10093 0

3 M LIM S I D GLP10-01-02-09-02 5 GLP10-01-02-09-02 6 GLP10-01-02-09-02 7 GLP10-01-02-09-02 8 GLP10-01-02-09025 ; 5000pp b LM S Descriptio n DA L G WGRS0 4 0 10093 0 DA L G WGRS0 4 D B 10093 0 DA L G WGRS0 4 L S 10093 0 DA L G WGRS0 4 H S 10093 0 DA L GWGRS0 4 010093 0 Averag e Concentratio n (ng/mL ) ±%RP D PFB S Concentratio n (ng/mL ) 236 0 245 0 NA'^ ' 445 0 769 0 VoRecover y

N A N A NA<^ > 40. 9 <^ > 10 6 241 0 ng/m L ±3.7 % PFH S Concentratio n (ng/mL ) 778 0 747 0 NA<^ > 944 0 1330 0 VoRecover y

N A N A NA<^ ' 36. 3 '^ ' 11 4 763 0 ng/m L ±4.1 % PFO S Concentratio n (ng/mL ) 131 0 143 0 NA<^ ' 241 0 574 0 VoRecover y

N A N A NA'^ > 20. 8 '^ ' 87. 4 137 0 ng/m L ±8.8 % N A= No t Applicabl e NC = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2xspik e level
. Samples dilute d 500

xan d analyze d o n 2/11/11
. (1 ) Th e L S sampl e was no t dilute d a s th e H S was th e mor

e appropriate spik

e fo rPFB Aan d PFHS
. (2 ) Fiel d matri xspik

e di d not mee

t method acceptance criteri a of100 % ±30%
. 

### Tabl e 16
. DA L G W3 1 O R 10092 4

3MUMSI D GLP10-01-02-09-02 9 GLP10-01-02-09-03 0 GLP10-01-02-09-03 1 GLP10-01-02-09-03 2 Descriptio n DA L GW310 R 010092 4 DA L G W3 1 O R D B 10092 4 DA L G W3 1 O R L S 10092 4 DA L GW310RH S 10092 4 Averag e Concentratio n (ng/mL ) ±%RP D PFB S Concentratio n (ng/mL ) 44 8 45 4 56 3 144 0 VoRecover y

N A N A N C 98. 9 45 1 ng/m L ±1.3 % PFH S Concentratio n (ng/mL ) 46 0 44 6 56 3 146 0 VoRecover y

N A N A N C 10 1 45 3 ng/m L ±3.1 % PFO S Concentratio n (ng/mL ) 96 1 82 9 86 3 192 0 VoRecover y

N A N A N C 10 3 89 5 ng/m L ±15 % NA= No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2xspike level
. Sample s dilute d lOO

xan d analyze d 10/25/10
.

Pag e 2 6 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### Tabl e 17
. DA L G W312 R 10093 0

3 M LIM S I D GLP10-01-02-09-03 3 GLP10-01-02-09-03 4 GLP10-01-02-09-03 5 GLP10-01-02-09-03 6 Descriptio n DA L GW312 R 010093 0 DA L GW312RD B 100930 DA L GW312RL S 100930 DA L GW312RH S 100930 Averag e Concentratio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 142 0 141 0 246 0 628 0 VoRecover

y N A N A 105 97. 3 1420ng/m L ±0.71 % PFH S Concentratio n (ng/mL ) 83 4 84 9 192 0 617 0 VoRecover

y N A N A 10 8 10 7 84 2 ng/m L ±1.8 % PFO S Concentratio n (ng/mL ) 147 0 155 0 237 0 674 0 VoRecover

y N A N A 86. 0 105 151 0 ng/m L ±5.3 % N A= No t Applicabl e Sample s dilute d lOO

xan d analyze d 10/25/10
. 

### Tabl e 18
. DA L G W317 L 10092 4

3 M LIM S I D GLP10-01-02-09-03 7 GLP10-01-02-09-03 8 GLP10-01-02-09-03 9 GLP10-01-02-09-04 0 Descriptio n DA L GW317 L 010092 4 DA L GW317LD B 100924 DA L GW317LL S 100924 DA L G W317LH S 100924 Averag e Concentratio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) <0.25 0 0.32 4 1.2 0 9.7 6 VoRecover

y N A N A 87. 6 94. 4 0.32 4 ng/mL^ * PFH S Concentratio n (ng/mL ) 0.23 6 0.23 7 1.22 9.9 3 VoRecover

y N A N A 98. 4 96. 9 0.23 7 ng/m L ±0.4rAo PFO S Concentratio n (ng/mL ) <0.50 0 <0.50 0 1.2 0 8.5 2 VoRecover

y N A N A 12 0 85, 2 < 0.50 0 ng/m L N A= No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2xspik e level
. Sample s wer

e analyze d 11/8/10
. (1 ) Asample/sampl e duplicate RP D coul d no t b e calculate d since on e sampl e replicate had a resul t ofBLOQ
.

Pag e 2 7 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### Tabl e 19
. DA L G W324 L 10092 9

3 M LIM S I D GLP10-01-02-09-04 1 GLP10-01-02-09-04 2 GLP10-01-02-09-04 3 GL P 10-01-02-09-04 4 Descriptio n DA L GW324 L 010092 9 DA L GW324LD B 10092 9 DA L GW324LL S 10092 9 DA L GW324LH S 10092 9 Averag e Concentratio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 89. 1 89. 5 18 6 97 8 VoRecover

y NA NA 96. 7 88. 9 89. 3 ng/m L ±0.45 % PFH S Concentratio n (ng/mL ) 10 5 11 4 21 6 114 0 VoRecover

y N A N A 107 103 11 0 ng/m L ±8.2 % PFO S Concentratio n (ng/mL ) 24 9 26 4 35 9 128 0 VoRecover

y N A N A N C 102 25 7 ng/m L ±5.8 % NA = No t Applicabl e NC = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2xspik e level
. Samples dilute d 20

xan d analyze d 10/25/10
. 

### Tabl e 20
. DA L GW327 R 10093 0

3 M LIM S I D GLP10-01-02-09-04 5 GLP10-01-02-09-04 6 GLP10-01-02-09-04 7 GLP10-01-02-09-04 8 Descriptio n DA L GW327 R 010093 0 DA L GW327 R D B 100930 DALGW327RL S 100930 DALGW327RH S 100930 Averag e Concentratio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 12 5 12 7 23 5 111 0 VoRecover

y N A N A 10 9 98. 4 12 6 ng/m L ±1.6

% PFH S

Concentratio n (ng/mL ) 22 7 23 1 33 7 134 0 VoRecover

y N A N A N C 11 1 22 9 ng/m L ±1.rA o PFO S Concentratio n 

### Jng/mL) 67 2 67 7 76 1 193 0 VoRecover

y N A N A N C 12 6 67 5 ng/m L ±0.74 % NA= No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2xspik e level
. Samples dilute d 50

xan d analyze d 10/25/10
.

Pag e 2 8 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### Tabl e 21
. DA L GW328 R 10092 8

3 M LIM S I D GLP10-01-02-09-04 9 GLP10-01-02-09-05 0 GLP10-01-02-09-05 1 GLP10-01-02-09-05 2 Descriptio n DA L GW328 R 010092 8 DA L GW328RD B 10092 8 DA L GW328RL S 10092 8 DA L GW328RH S 10092 8 Averag e Concentratio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 31. 8 31. 7 13 7 49 1 VoRecover

y N A N A 105 10 1 31. 8 ng/m L ±0.31 % PFH S Concentratio n (ng/mL ) 66. 3 69. 7 18 3 56 1 VoRecover

y NA N A 11 5 10 9 68. 0 ng/m L ±5.0 % PFO S Concentratio n (ng/mL ) 17 1 165 26 1 63 2 VoRecover

y N A N A 93. 0 10 2 168ng/mL±3.6V o NA= Not Applicabl e Samples dilute d 20

xan d analyze d o n 10/25/10
. 

### Tabl e 22
. DA L GW328 L 10092 8

3 M LIM S I D GLP10-01-02-09-05 3 GLP10-01-02-09-05 4 GLP10-01-02-09-05 5 GLP10-01-02-09-05 6 Descriptio n DA L GW328 L 010092 8 DA L GW328LD B 10092 8 DA L GW328LL S 10092 8 DA L GW328LH S 10092 8 Averag e Concentratio n (ng/mL ) ±%RP D PFBS^ > Concentratio n (ng/mL ) 62. 1 64. 0 72. 6 16 7 VoRecover

y NA NA N C 104 63. 1 ng/m L ±3.0 % PFHSf ' Concentratio n (ng/mL ) 22. 5 24. 6 32. 3 13 1 VoRecover

y NA NA N C 107 23. 6 ng/m L ±8.9 % PFOS ^ Concentratio n (ng/mL ) 0.72 2 0.64 9 9.8 3 69. 7 VoRecover

y N A N A 10 2 69. 0 <^ ' 0.68 6 ng/m L ±11 % N A= No t Applicabl e N C = Not Calculated ; Endogenou s sampl e concentratio n greate rtha n 2 xspike level
. (1 ) Sample s dilute d 20

xan d analyze d o n 10/25/1 0 fo rPFB S an d PFHS
. (2) Sample s analyze d o n 3/15/1 1 fo rPFOS , (3) Fiel d matrixspike di d no t meet method acceptance criteri a of100 % ±30%
.

Pag e 2 9 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 /^alysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### Tabl e 23
. DA L GW330 R 10092 3

3 M LIM S I D GLP10-01-02-09-05 7 GLP10-01-02-09-05 8 GLP10-01-02-09-05 9 GLP10-01-02-09-06 0 Descriptio n DA L GW330 R 010092 3 DA L GW330RD B 10092 3 DALGW330RL S 10092 3 DA L GW330 R H S 10092 3 Averag e Concentratio n (ng/mL ) ±%RP D PFB S Concentratio n (ng/mL ) 281 0 275 0 375 0 761 0 VoRecover

y N A N A N C 96. 6 278 0 ng/m L ±2.2 % PFH S Concentratio n (ng/mL ) 33 1 32 9 138 0 NA'^ ' VoRecover

y NA N A 105 NA<^ ' 33 0 ng/m L ±0.61 % PFO S Concentratio n (ng/mL ) 80 3 80 1 178 0 NA<^' VoRecover

y N A N A 97. 8 NA<^ ) 80 2 ng/m L ±0.25 % N A= No t Applicabl e Samples were dilute d 50

xfo rPFH S an d PFOS , an d lOO xfo rPFB S an d analyze d o n 10/25/10
. (1 ) Sampl e concentratio n exceede d calibratio n range . Afurthe rdilutio n was no t prepare d as th e LS sampl e was th e mor e appropriate spik

e leve l fo rPFH S an d PFOS
. 

### Tabl e 24
. DA L GW330 L 10092 3

3 M LIM S I D GLP10-01-02-09-06 1 GLP10-01-02-09-06 2 GLP10-01-02-09-06 3 GLP10-01-02-09-06 4 Descriptio n DA L GW330 L 010092 3 DA L GW330LD B 10092 3 DA L GW330LL S 10092 3 DA L GW330LH S 10092 3 Averag e Concentratio n (ng/mL ) ±%RP D PFB S Concentratio n (ng/mL ) 61 9 65 2 67 7 151 0 VoRecover

y N A N A N C 97, 8 63 6 ng/m L ±5.2 % PFH S Concentratio n (ng/mL ) 49 8 45 4 56 0 168 0 VoRecover

y NA NA N C 135'^ ' 47 6 ng/m L ±9.2%' ^ PFO S Concentratio n (ng/mL ) 19 2 15 1 24 8 127 0 VoRecover

y N A N A 84. 5 12 3 172 ng/m L ±24%'^ ' N A= No t /Applicabl e N C = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2xspike level
. Samples dilute d 20

xan d analyze d o n 10/25/10
. (1 ) Fiel d matri xspik

e di d not mee

t metho d acceptanc

e criteri a of100 % ±30%
. (2 ) Th e analytica l uncertainl yha s bee n adjuste d fo rFM S recoveryfo rPFH S to ±35%
. (3) Th e RP D di d no

t meet method acceptance criteri a ofS20%
.

Pag e 3 0 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s ofPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### Tabl e 25
. DA L G W331 S 10093 0

3 M LIM S I D GLP10-01-02-09-06 5 GLP10-01-02-09-06 6 GLP10-01-02-09-06 7 GLP10-01-02-09-06 8 Descriptio n DA L GW331 S 010093 0 DA L GW331SD B 100930 DA L GW331SL S 100930 DA L GW331SH S 100930 Averag e Concentratio n (ng/mL ) ±VoRP D PFB S Concentratio n (ng/mL ) 92 7 92 2 107 0 199 0 VoRecover

y N A N A N C 107 92 5 ng/m L ±0.54 % PFH S Concentratio n (ng/mL ) 61 1 61 1 78 8 177 0 VoRecover

y N A N A N C 11 6 61 1 ng/m L ±0.0 % PFO S Concentratio n (ng/mL ) 117 0 119 0 167 0 239 0 VoRecover

y N A N A N C 12 1 118 0 ng/m L ±1.7 % N A= No t Applicabl e N C = No t Calculated ; Endogenou s sampl e concentratio n greate rtha n 2 xspik e level
. Samples dilute d 50

xan d analyze d o n 10/25/1 0 

### Tabl e 26
. Tri p Blan k

1

3 M LIM S I D GLP10-01-02-09-06 9 GLP10-01-02-09-07 0 GLP10-01-02-09-07 1 GLP10-01-02-09-07 2 Descriptio n Tri p Blan k

1 Sampl e Tri p Blan k

1 FM S Low Tri p Blan k

1 FM S Mi d Tri p Blan k

1 FM S Hig h PFB S

Concentratio n (ng/mL ) <0.25 0 10. 7 10 8 110 0 VoRecover

y N A 107 10 8 11 0 PFH S

Concentratio n (ng/mL ) <0.10 0 11. 4 11 4 116 0 VoRecover

y N A 11 4 11 4 11 6 PFO S

Concentratio n (ng/mL ) <0.99 9 11. 4 102 108 0 VoRecover

y N A 11 4 102 10 8 NA = No t Applicabl e FM S Mi d dilute d lOx , FM S Hig h dilute d 50x , an d analyze d o n 10/25/10
.

Pag e 3 1 of8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### Tabl e 26
. Tri p Blan k

2

3 M LIM S I D GLP10-01-02-09-07 3 GLP10-01-02-09-07 4 GLP10-01-02-09-07 5 GLP10-01-02-09-07 6 Descriptio n Tri p Blan k

2 Sampl e Tri p Blan k

2 FM S Low Tri p Blan k

2 FM S Mi d Tri p Blan k

2 FM S Hig h PFB S

Concentratio n (ng/mL ) <0.25 0 10. 8 10 8 110 0 VoRecover

y NA 10 8 10 8 11 0 PFH S

Concentratio n (ng/mL ) <0.10 0 11. 4 11 5 119 0 %Recover

y N A 11 4 11 5 11 9 PFO S

Concentratio n (ng/mL ) <0.99 9 11. 0 99. 6 109 0 VoRecover

y N A 11 0 99. 6 10 9 NA= No t Applicabl e FM S Mi d dilute d lOx , FM S Hig h dilute d 50x , an d analyze d o n 10/25/10
. 

### Tabl e 27
. Rinseat e Blan k

3 M LIM S I D GL P 10-01-02-09-07 7 Descriptio n DA L GW220RR B 100930 PFB S Concentratio n (ng/mL ) <0.25 0 VoRecover y NA PFH S

Concentratio n (ng/mL ) <0.10 0 VoRecover y N A PFO S

Concentratio n (ng/mL ) <0.99 9 %Recover y N A NA= No t Applicabl e Sampl e was analyze d o n 10/25/1 0

Pag e 3 2 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



## 1 0 Conclusio n Laborator ycontro l spike s an d fiel d matri xspike s were use d t o detennin e th e analytica l metho d accurac yan d precisio n fo rPFBS , PFHS , an d PFOS
. Analysi s wa s successfull ycomplete d followin g 3 M Environmenta l Laborator ymetho d ETS-8-044. 0 describe d herein
. 1 1 Data/Sampl e Retentio n Al l remainin g sample s an d associate d projec t data (hardcopyan d electronic) wil l b e archive d accordin g t o 3 M Environmenta l Laborator ystandar d operatin g procedures
. 1 2 Attacfiment s Attachmen t A : Protoco l Amendmen t 0 9 (Genera l Projec t Outline ) Attachmen t B : Representative Chromatogram s an d Calibratio n Curve s Attachmen t C : Analytica l Metho d Attachmen t D : Metho d Deviatio n

Pag e 3 3 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### 13^^Signature s

Date Clesto n Lange , Ph.D. , 3 M Principa l Analytica l Investigato r j?j-Ai^;^e>i7::^jorA / Willia m K
. Reagen , Ph.D. , 3 M Environmenta l Laborator yDepartmen t Manage rDate



# 3
. / ^ t 

# 11 Gar yHohensteun , Sponso rRepresentative 

# fJilAU / Date

I I Date Jaisimh a Kesari , Stud yDirecto r

Pag e 3 4 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0


### ATTACHMEN TA : PROTOCO L AMENDMEN T

Pag e 3 5 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sept 201 0 

### Analytica l Protocol : GLP10-01-0 2

Amendmen t 9



## Stud yTitl e Analysi s ofPerfluorooctan e Sulfonat e (PFOS) , Perfluorohexan e Sulfonat e (PFHS ) an d Perfluorobutan e sulfonat e (PFBS ) i n Groundwater , Soi l an d Sedimen t fo rth e 3 M

Decatu rPhas e 3 Site-Relate d Monitorin g Progra m

PROTOCO L AMENDMEN TNO
. 9

Amendmen t Date : Septembe r13 , 201 0 Performin g Laborator y 3 M Environmental , Health , an d SafetyOperation s

3 M Environmenta l Laborator y Buildin g 260-5N-1 7 Maplewood , M N 55144-100 0
Laborator yProjec t Identificatio n

GLP10-01-0 2

Pag e 1 of7 Pag e 3 6 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sept 201 0 

### Analytica l Protocol : GLP1Q-01-02

Amendmen t 9 

## Thi s amendmen t modifie s th e foilowin g portio n o fprotocol : "Analysi s o fPFOS , PFH S an d PFB S i n Groundwater , Soi l an d Sedimen t fo rth e 3 M

Decatu rPhas e 3 Site-Relate d Monitorin g Program "

PROTOCO L READS : 

## N o change s t o th e wordin g o fth e protoco l are required
.

AMEN D TO READ : 

## N o change s t o th e wordin g o fth e protoco l ar e required
. Thi s amendmen t onl y addresse s an d document s th e additio n o fth e Genera l Project Outlin e (GPQ ) fo rth e collectio n an d analysi s o fgroundwate rsample s fro m Decatur
. AL
. an d conducte d a s par t ofth e 3 M Decatu rPhas e 3 Progra m fo rPFOS
. PFH S an d PFB S (GLP10-01-02)
. Th e anticipate d sampl e collectio n wil l occu raroun d th e timefram e o fSeptembe r20
. 2010
. Th e groundwate rsample s fo rthi s samplin g even t wil l b e entere d int o th e 3 M Environmenta l Laborator yLIM S a s project GLP10-01-01-0 9 an d reporte d a s interi m repor t GLP10-01-02-09 , (reflectin g stud yGLP10-01-0 2 an d amendmen t -09)
.
Reason : 

## Th e reaso n fo rthi s amendmen t i s t o documen t th e Genera l Project Outlin e (GPO ) whic h describe s th e anticipat e groundwate rsampl e collectio n even t t o b e conducte d fo r th e 3 M Decatur , A L facility
. Th e GP O i s fou rpage s i n lengt h an d include d a s attache d t o thi s amendmen t form
.

Pag e 2 of7 Pag e 3 7 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0 

### Analytica l Protocol : GLP10-01-0 2

Amendmen t 9 

## Amendmen t Approva l 

# U fi^%^^f i 

# y^/ 0 Gar yHohenstein , Sponso rRepresentative Date

Clesto n C
. Lange , Principa l Analytica l Investigato r 

# V/5/
. Z«f/2 > Date 

# P ^ S^/'^o/ O Willia m Reaoen , EH S Opn s Environmenta l La b Managemen t Date



# m m Jaisimha^<esar i P.E. , DEE , Stud yDirecto r Date

Pag e 3 o f7 Pag e 3 8 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S I n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0 Analytica l Protocol : GLP10-01-0 2

Amendmen t 9 

## Environmenta l Healt h & Safet yOperations , Environmenta l Laborator y

Genera l Projec t Outlin e To : Gar

yHohenstein , S M EHS&Opn s From : Susa n Wolf , 3 M EHS&Opns ; Environmenta l La b cc : Willia m Reagen , 3 M EHS&Opns ; Environmenta l La b

Clifflo n Jacoby , 3 M EHS&Opns ; Environmenta l La b Clesto n Lange , 3 M EHS&Opns ; Environmenta l La b Ja i Kesari , Westo n Solution s Charie s Young , Westo n Solution s Ti m Frinak , Westo n Solution s Date ; Septembe r13,201 0 Subject : Analysi s o fPerfluorooctan e Sulfonat e (PFOS) , Perfluorohexan e Sulfonat e (PFHS ) an d Perfluorobutan e

sulfonat e (PFBS ) i n Groundwater , Soi l an d Sedimen t fo rth e 3 M Decatu rPhas e 3 Site-Relate d Monitorin g Program ; GL P Interi m Repor t 0 9 - Decatu r3"

^ Quarte rGroundwate rSamplin g 

## 1 Genera l Projec t Informatio n

Contact s

La b Reques t Numbe r SixDigi t Departmen t Numbe r Projec t Schedule/Tes t Date s 3 M Sponso rRepresentativ e Gar yHohenstei n S M EH S Operation s 3 M Buildin g 224-5W-0 3 Sain t Paul , M N 55144-100 0 Phone:(651)737-357 0 aahohensteinSJmmm.co m 3 M Environmenta l Laborator yIVIanagemen t Willia m K
. Reage n 3 M EH S Opns , Environmenta l Laborator y 260-5N-1 7 65 1 733-973 9 wkreaaenOmmm.co m Principa l Analytica l investigato r Clesto n Lang e 3 M EH S Opns , Environmenta l Laborator y 260-5N-1 7 65 1 733-986 0 cclanae(a)mmm,co m Samplin g Coordinato r Timoth yFrina k Westo n Solution s Timothv.frinak(5!westonsolutions.co m Phone ; (334)-332-912 3 GLP10-01-02-0 9 Dept #530711
. Project #002267444 9 Samplin g schedule d fo rth e wee k o fSeptembe r20 , 201 0 Al l verba l an d writte n correspondenc e wil l b e directe d t o Gar yHohenstein
.
Pag e 4 o f7 Pag e 3 9 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S I n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0 

### Analytica l Protocol : GLP10-01-0 2

Amendmen t 9 2 Backgroun d Informatio n an d Projec t Objective(s ) Th e 3 M EH S Operation s Laborator y(3 M Environmenta l Lab ) wil l receiv e an d analyz e groundwate r sample s collecte d fro m seventee n groundwate rwell s fo rPerfluorobutanesulfonat e (PFBS) , Perfluorohexanesulfonat e (PFHS) , an d Perfluorooctanesulfonat e (PFOS)
. Analyse s wil l b e conducte d unde rth e GL P requirement s o fEPATSC AGoo d Laborator yPractic e Standard s 4 0 CF R 792
. Groundwate rsample s fro m Decatur , A L wil l b e collecte d byWesto n Solution s personne l th e wee k o f Septembe r20 , 2010
. Th e 3 M Environmenta l Laborator ywil l prepar e th e sampl e bottle s wit h al l require d spike s t o ensure tha t result s fo rPFBS , PFHS , an d PFO S ar e o fa know n precisio n an d accuracy . Th e fina l repor t wil l b e submitte d t o Gar yHohenstei n an d Ja i Kesar i upo n completio n unde rinteri m repor t GLP10 - 01-02-09
. 

## 3 Projec t Schedul e Sampl e collectio n bottle s wil l b e prepare d b y3 M Environmenta l Laborator yfo rsamplin g th e wee k

ofSeptembe r 20,2010
. Sampl e bottle s wil l b e shippe d i n cooler s ovemigh t t o 3 M Decatu rfo ramVa l b yFriday , Septembe r 17,2010
. Sampl e bottle s shoul d b e store d refrigerate d on-sit e unti l sampl e collection
. Marti n Smit h \

Westo n Traile r 3 M Decatu rPlan t 140 0 Stat e Docks Roa d Decatur , Alabam a 3560 1 

## 4 Tes t Parameter s Th e targete d limi t ofquantitatio n wil l b e 0.02 5 ng/m L (ppb ) fo rPFBS , PFHS , an d PFOS
. Atota l o fseventee n samplin g location s have bee n specified
. Fo reac h samplin g location , fou rsampl e bottJe s wil l b e collecte d (sample , sampl e duplicate , lowfiel d matri xspike , an d hig h fiel d matri xspike)
. Th e "fil l to here " lin e o n eac h 25 0 m L Nalgen e bottl e wil l b e 20 0 mL
. Fou rsets o ftri p blan k spike s consistin g o freagent-gi^d e water , as wel l a s a n additiona l bottl e to be use d fo rth e preparatio n o fth e equipmen t rinseate blanks , wil l b e prepare d a

t th e 3 M Environmenta l Laborator yan d sen t t o th e samplin g locatio n wit h th e othe rbottles
. Results fro m GLP10-01-02-0 2 were use d t o detemiin e th e fiel d matri xspik e level s fo rGLP10-01-01-0 9 liste d i n Tabl e 1
. Atota l o f8 5 sampl e bottle s wil l b e prepared
. Tabl e 1
. Samplin g Location s an d Fiel d Matri xSpik e levels
. Wel l No
. 317 L 220R , 220L , 328 L 203L , 222R , 227L , 227R
. 310R,324L , 327R
. 330L , 331 S GRS04 , 312R , 330 R 328 R Tri p Blan k

Set s 1,2 , 3 , an d 4 Sampl e Leve l Low Hig h Low Hig h Low Hig h Low Hig h Low Hig h Low Mi d Hig h Spik e Cone
. (ng/mL ) 1 1 0 1 0 10 0 10 0 100 0 100 0 500 0 10 0 50 0 1 0 10 0 100 0


### Pag e 5 of7 Pag e 4 0 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0 

### Analytica l Protocol : GLP10-01-0 2

Amendmen t 9

5 Tes t Method s Sample s wil l b e prepare d an d analyze d byLC/MS/M S followin g ETS-8-044. 0 "Determinatio n ofPerfluorinate d Compound s I n Wate rbyHig h Performance Liqui d Chromatography/Mas s Spectrometr yDirec t Injectio n Analysis"
. Du e t o previou s dat a fo rthes e samplin g location s (GLP10-01-01-02 ) sample s wil l most likel yrequir e dilution
. Sample s requirin g dilutio n wil l b e prepare d i n Milli- Q wate rprio rt o analysi s byETS-8-044.0
. Th e data qualit yobjective s fo rthes e studie s ar e quantitative results fo rth e target analyte s wit h a n analytica l accurac yof 100+30%
. Fiel d matri xspike s no t yieldin g recoverie s withi n 100±30 % wil l b e addresse d i n th e repor t an d th e fina l accurac

ystatemen t ma

yb e adjuste d accordingly
. Alternately , sample s ma

yb e analyze d b

yETS 8-110. 1 "Analysi s o fFluorochemical s i n Extracts Usin g HPLC Electrospray-Mas s Spectrometry-Mas s Spectrometry " as a n analytica l reference fo rth e sampl e location s analyze d bydirec

t on-colum n injectio n analysi s ofsample s dilute d wit h methano l as oppose d t o Milli- Q water
. Calibratio n curve s use d fo rbot h ETS-8-044. 0 an d ETS-8-110. 1 wil l b e constructe d usin g th e linea risome rof PFO S only , fro m nomina l concentration s of0.02 ng/m L -10 0 ng/mL
. Du e t o th e expecte d concentratio n of linea r+ branche d PFO S i n th e samples , there ma yb e th e potentia l fo ranalytica l bias du e t o quantitatin g linea r an d branche d isomer

s o fPFO S against a predominatel ylinea rreference standard fo rPFO S a t th e highe ren d o fth e calibratio n curve . Laborator ycontro l sample s (LCS ) oflinea r+ branche d PFO S wil l b e prepare d a t thre e concentration s t o evaluat e th e potentia l fo ranalytica l bias
. LC S wit h averag e recoverie s greate rtha n 100%±30%ma

ysuggest analytica l bias
. I fth e recover yo fth e linea r+ branche d LC S i s greate rtha n 100%±30 % i t ma

yb e necessar

yt o quantitat e PFO S byprocessin g th e linea ran d branche d isomer s separatel y an d summin g th e calculate d concentration s togethe rto generat e th e fina l PFO S concentraton
. Thi s ma yb e don e t o hel p minimiz e an ypotentia l analytica l bias du e to quantitatin g linea ran d branche d isomer s o fPFO S against a predominatel ylinea rreference standar d fo rPFOS
. 6 Reportin g Requirement s Fo reac h samplin g location , th e repor t wil l contai n th e results fo rth e sample , sampl e duplicate , an d th e fiel d matri xspikes
. Tri p blan k

sets wil l b e reporte d fo rth e samplin g even t as wil l an yequipmen t blank s prepare d i n th e field
. Laborator

ycontro l spike s ofreagen t wate rprepare d at th e tim e ofsampl e extractio n wil l als o b e reporte d an d use d t o evaluat e th e overal l metho d accurac yan d precision
. Metho d blank s ofreagen t wate r prepare d at th e tim e o fsampl e extractio n wil l b e use d t o detennin e th e metho d detectio n limit
. An ylaborator y matri xspike s that ma

yb e prepare d wil l als o b e include d i n th e fina l report
. 7 Emai l Correspondenc e Attachmen t A : Samplin g Bottl e Request

Pag e 6 o f7 Pag e 4 1 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0 

### Analytica l Protocol : GLP10-01-0 2

Amendmen t 9

LABORATOR

YREQUES

TFO R SAIVIPLE COI^AINER S

SIT E PROJECT; PROJE a NO
. SHIPPIN G ADDRES S

SHIPMEN TREQUIRE D BY: PROJEC TCONTACT ; CONTAC TNUMBER ; EMAIL ; Confidentia l Client , Decatur , Alabam a

3R D QUARTE R GROUNDWATE R SAMPLIN G

02181.129.145.000 3 Marti n Smit h - WESTO N TRAILE R 3 M DECATU R PUN T 140 0 STAT E DOCK

S ROA D DECATUR , A L 3560 1 256-552-618 9 20-Sep-l O TI M FRINA K OFFICE : 334-466-5653 ; CEL L334-332-912 3 t.frinak(S)we5tonsolutions.co m Sample s 1 7

1 4 Sampl e Typ e

Groundwate r G WDuplicat e Fiel d Blanks Rinsat e Sample s Tri p Blank s Paramete r

FC s

FC s FC s La b H2 0

Require d

Ye s Container s

COC s Require d USDAPermi t 62 5 an d Ubel s Require d MS/MSD s Selecte d b yLa b Preserv
.

VES N o YES

COMMENTS : Pleas e pac k

t o preven t breakage
. Pleas e provid e th e laborator yProjec t Numbe rfo rreferenc e o n th e COC
. On e tri p blan k

wil l b e use d pe rcoole rshipped
. Un-use d tri p blanks wil l b e returne d t o th e laborator yfo rdisposal
. 203 L 220 R 220 L 222 R 227 R 227 L 6RS0 4

310 R RW312 R 317 L 324 1 RW327 R WEL L LIS T 328 R 328 L 330 R 330 L RW331 S



### Pag e 7 of7 Pag e 4 2 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0


### ATTACHIVIEN TB : SAIVIPL E CHROMATOGRAIVI S AN D CALIBRATIO N CURV E

Pag e 4 3 o f8 3Buste r

J293020 3 GLP10-01-02 ; Interi m Repor t 0 9 Bat^pa|ym? f?ffl%2'5Et^§t?" d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0

I Sampl e Name : •b101025b02 rSampj a ID : -06008-194-10 - File : -b101025b.v ^ Pea k Name : 'PFBS * liAass<es) : -299.0/99. 0 «mu,299.0AO. O anuJ I Commen t "5. 0 ng/m L F C lt d i n M U Q \AibtBr ' Anrwtation ; ~

.- . I^anua l I Time : 12. S 193026 4 CO

I 3.736+00 5 Sampl e Name : -blO'tD25b034 ' Sampl e ID : ' I Pea k Name : *PFBS - Massfea) : *299.0/99. 0 * i I Commen t *20pp b LCS " Annotation: "

Acq . Date : Acq . Time : Modified : R T Window : Expacce d RT : Us e Relativ e RT : Int . Type : Retentio n Time : 10/26 / 3:27: 2 Ve s 30. 0 12 . N o Manu a 12 . Area : 62BS11 9 Heiqhl: : Star t Time : En d Time : .19a-* 0 12 . 13 . 1.15e6

- 1.10e6

- 1.05e6

- I.OOe S 9.50e 5 S.OOe S B.50e 5 8.00e 5 7.50e 5 7.00e 5 i 6.50e S j ^6.00e 5 I 5.50e 5 5.00e 5 4.50e 5 4.00* 5 3.50e 5 3.00e 5 2.50e S 2.00e 5 1.50e5 I.OOe S 5.00e 4 0.00 

# I Sampl e Name : "^010256045 - Sampl e ID : •LCS-101D2O-10 rFile : -b101025b.wf r Pea k Name : 'PFBS * MasB<ei) : *299.0/99. 0 amu,299.0«0. 0 amu Commen t "20pp b LC S ECF * Annotation : ~ Sampl e Index : A b

rvp e 0.0 0 ng/m L 0.21 9 ng/m

L 10/26/201 0 7:19:0 4 A

H

Heiglit : Star t T i En d Tim e 2. 1 » 4 2.0e 4 1.9» 4 1.8e 4 1.7e4 ' 1.6e 4 1.Se4 ' 1.4 ^ 1.3e 4 1.2e4 - 1.1e4 ' 1.0e 4 9000. 0 SOOO.O 7000. 0 6000.0 - 5000. 0 4000. 0 3000.0 - 2000. 0 1000. 0 0. 0 

# I Sampl e Name : -b101025b04? ' Sampl e ID : ' Pea k Name : -PFBS " Ma««(o«) : -299.0»9. 0 « i Commen t -Metho d Blank * Annotation : ~ Sampl e Type : Unknow n Concentration : N/ A Calculate d Cone : - 0 Acq
. Date : 10/25/201 0 Ftte : "bl01025b.wif r

R TKi t E.-pscce d i l'3 S Relat i Int
. Type^ Retentio n Area : Height : 'ea k Heigti i 'ea k Widtl l lin g Widtrf

i chm : IntelliCu ;



### ...AyiAv*Utol»Mtt»4*j4_..
. 1 6 1 8 1 2 1 4 1 6 1 8 Printin g Time : 1:32:4 2 P M Printin g Date : Tuesday , Februar y08 , Pag e 1 o f 6 Pag e 4 4 o f8 3** * Buste r

J293020 3 GLP10-01-02 ; Interi m Repor t 0 9 Bat^R3!ym? f?m^?§HcSi?" d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0 

# [ Sampl e Name : 'b101025b050 " Sampl e ID : Pea k Name : "PFBS ' Mass{es) : -299.0/99. 0 Commen t 'DA L G W203 L 0 10092 9 (20X) * Sampl e Type : Concentration : Calculate d Cone : Acq
. Time : Hsdified : R TV/indow : Expecte d RT : Us e Relativ e RT : N/ A 70. 1 ng/m

L 10/2e/201 0 9:0<:1 3 A

M •GLP1&O1-O2-09-O0 r

amu , 299.0/80. 0 amu ' Annotation:" "
Z5e5 - Z4e 5 2.3e5 - 2.2e 5 2. 1 e 5 • 2.0e 5 1.9« 5 1.8«5 - 1.7e 5 1.6e 5 1.Se5 - 1.4« 5 1.3« 5 1.2e 5 1.1« 5 I.OeS 9.0e 4 8.0e 4 7.0e 4 6.De4 - 5.De4 - 4.0e 4 3.0e 4 ^Oe 4 1.0o 4 Ftte : t l 01025b.wi r

0. 0 l ^ 

# s Sampl e T Calcula t Acq
. Dat Acq
. Ti m Modifie d R T Kindo

Expecte d Us e Rel a tion :

dn

RT : i v e n c !

KT : Unknow

n H/ A 115
. 10/26/201 0 4:;5:5 5 P

M Y« s 30. 0 12. 6 N o 

# I Sampl e Name : 'b101025bO71 ' Sampl e ID : "GLP10^)1-02-09^) 1 rFite:"b101025b.wi r Pea k Name ; "PFBS - Mass(ea) ; -299.0ra9. 0 amu,299.0/80. 0 amu ' Comment * DALGW222R0100929{50X) - Annotation : " Sampl e Index : 7 1
1.7e 5 1.6e 5 1.5e 5 1.4e5 - 1.3e 5 1Ji« 5 1. 1 e S 1.0e 5 9.0»4 - B.0e4 - 7.0e 4 6.0e 4 5.0e 4 4.0»4 - 3.0e 4 Z0e 4 1.0e4 - 0. 0 JL. ^ 

# I Sampl e Name : -b101025b128 - Sampl e 10 : 'GLP1CM)1-02-0»O49 - Pea k Name ; -pFBS " Ma»«(B») : -299.0/99.0amu,299.0/80. 0 amu " ConvTwn fDALGW328R0100S28{20X) - AnnotoSon : ~ Sampl e Index : 12 9 Sampl e Type : File:-b101025b.wf r 

# [ Sampl e Name : "b101025b02 rSampl e ID : •08008-194-10 ' File : "b101025b.wi r Pea k Name : "PFHS - Mass{as} : -399.Qra9. 0 amu,399.0/80. 0 amu " Commen t -5. 0 ng/m L F C tt d i n M M Q Wbter " Anrwtation : "
tio
.

Modified : R T Window : Expecte d R T Us e Relativ . N/ A

Heiijht : Star t T i En dTim ? 1.20e 5 1.15e 5 1. 1 Oe 5 I.OSeS I.OOe S 9.50e 4 9.00e 4 8.50e 4 6.0De 4 7.50e 4 7.00* 4 § - 6.S0e 4 ^ 6.00e 4 I 5.50e

4 5.00e 4 4.50e4 - 4.00e 4 3.50e 4 3.00e 4 Z50e 4 Z00e 4 1.50e 4 1.00e 4 5000.0 0 0.0 0 Sampl e Type : Standar d Concentration : S.O O Calculate dC Acq
. Date : Acq
. Tims : Modified : Proc
. Algcr i Min
. Peak

H e Min
. Psa k

wi Smoottiin o Hi R T Window : E.".pacts dRT : I's e Relativ e Int

- Type : Retentio nT i Area : Height : Star t Time : En dTime : nc : S.o;

- 10/25/201 0 10:5<:0 6 P

H N o hm : IntelliQ'ja n ght : D.0 0 c ttl ! 0.0 0 3 •th : 0 p o

30. 0 = H. 2 t

n RT : N

o Valle y tie : IJ. l m 165555 1 cou n J.SSe.OO S c H. O a H . i m

I D 1 2 Tim e mi n Printin g Time : 1:32:4 2 P M Printin g Date : Tuesday , Februar y 08 , 201 1 Pag e 2 o f 6 Pag e 4 5 o f8 3** * Buste r

J293020 3 GLPIO-01-02 ; Interi m Repor t 0 9 Bat^Ra|ym? feffi%2'5§t^^^k?" d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sept 201 0

Sampl e Tvpe : Concentration : Calculate dCone : Acq
. Date : Acq
. Time : Modified : Proc
. Algorithm : Min
. Pea k

Height : Min
. Pea k

Width ! Smoothin g width : R TWindow : Expecte dRT : Us e Relativ e RT : Int
. Type : Retentio nTiisa : Area : 53 < Height : 1 QC 20. 0 17. 3 10/2e/201 0 3:27:2 7 A

M N a IntelliQu a 0.0 0 0.0 0 0 p 30. 0 14. 2 N o Valle y 14. 1 12* 0 co u .ISetOO S 

# I Sampt e Name : •b101025b034 - Sampt a ID : 'LCS-101020-4 " Ftte : * b 101025b.wiff " Pea k Name : 'PFHS ' llAass(«s} : "399.0/99. 0 amu.3g9.0/80. 0 amij Commen t "20pp b LCS " Anrutation : ~
1.15e6 - I.IOeS I.OSeS I.OOeS 9.50e5 - 9.00e5 - 8.50e 5 a.OOe S 7.50e5 - 7.00e 5 6.50e5 - 6.00e 5 5.50e 5 S.OOe S 4.50e 5 4.00e5 - 3.50eS 3.00e 5 2.S0e 5 ZOOe S 1.50e 5 I.OOe S 5.00e 4 0.0 0 

# r Sampl e Name : "b101025b045 " Sampl e ID : "LCS-1D1020-10 - File : -b101025b.vrtf r Pea k Nanne : "PFHS - Masa(es) : "399.0/99. 0 amu.399.0/80. 0 emu " Commen t -20pp b LC S EOF " Annotation : " Sampl e Index : 4 5 Q C nq/ra L Sampl e Type : Concentrati o Calculate dC Acq
. Date : Acq , Time : llJdifiad : R TWindow : Expncte dRT : Us e Relativ e one :

RT ! O

C 0.0 0 1.1 1 10/26/201 0 7:19:0 4 A

M Ye s 30. 0 14. 2 N o



### 1



### |

- 7.0e 4
6.5e 4
6.0e 4
5.5e 4
5.0» 4
4.5e 4
4.0e 4
3.5e 4
3.0e 4
Z5e 4
ZDe 4
1.5e 4
1.0o 4

SOOO. O
D O 14.1 2 

# 1 Iu , A 

# A

. „ .<<^

,



### • Sampl e Name : "b101O2SbO49 - Sannpt e ID : "R O V*to rTN1CW311 " File : " b 101025b.wit T Pea k Name : "PFHS ' Uass(es) : "399.0/99. 0 amu,399.0/80. 0 ami T Convnen t 'Metho d Blank * Annotation : " Sampl e Index : 4 9 Sampl e Type : Unknow n Concentration : N/ A Calculate d Cone : < 0 * Acq
. Date : 10/26/201 C Accj
. Tima : 9:43:1 4 AM
Proc
. Alqoc i Min
. Pea k

H e Min
. Pea k

W i .hm : q h t •1th : Pmoothin g VJidth : R TWindow : E.xpecte dRT : Us s Relativ e Int
. Type : Retentio nT i Area : Height : Star t Titne : En dTime : KT : IntelliS u 0.0 0 0.0 0 0 30. 0 14. 2 N o Valle y 14. 1 136 5 C

O ;.70e+00 2 14. 1 14. 2 a n-

c p s poi n mi n

unt s c p s mi n mi n lO A

3

& 300 0 

### f
1 250 0

Ki.lf...LU
. I f- •^
. J
. ..ft-^.J.a^..
. J4fa-

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# I Sampl a Name : "b101025b050 " Sampl e ID : "GLP1CK)1-02-0*O0 rFile : •b101025b.wi r Pea k Name : 'PFHS - M««»(o«) : "399.0/99. 0 amu,399.0/80. 0 amu ' Commen t "DA L G W203 L 010092 9 (20X) - Annotation : - Sampl e Index : 5 0 Sanipl e Type : Unknow n Concentration : tl/ A Calculate d Cone : 4Se
. nq/ n Acq
. Date : 10/2S/201 0

Heiijlit : Star t T En dTi m Manua l

,e : 14. 1 70T438 0 : • 1.20e 6 1.15e 6 i.ioe e 1.05e 6 1.00e S 9.50o 5 9.00e S 6.50e 5 8.00e 5 7.50e 5 7.00e 5 6.50e 5 6.00e 5 5.50e 5 5.00* 5 4.50e 5 4.00e 5 3.50e 5 3.00o 5 2.50e 5 ZOOe S 1.S0e 5 LOOe S 5.00e 4 141 3

14.00
.

16.4 8 1 0 1 2 1 4 1 6 Time , mi n Printin g Time : 1:32:4 3 PM Printin g Date : Tuesday , Februar y 08 , Pag e 3 o f 6 Pag e 4 6 o f8 3•* • Buste r

J293020 3 GLPIO-01-02 ; Interi m Repor t 0 9 Bat^Ra!ym? f?m%2'5EH<&#" d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0 

# [ Sampt o Name:'b101O2SbO71 - Sampt a ID : •GLP1tW)1-02-<»«i rFJte : •b10102Sb,*rt r Pea k Name : "PFHS " MaM<ec} : "399.0/99. 0 aiTUJ.399.0/aO. O ami T Commen t "DA L GW222R010092 9 (50X rAnnolation: " Sampl e Type : Concentration : Calculate d Cone : Acq
. Date : Acq
. Tine : Modified : R TWindou : Expecte d RT : Us e Relativ e RT : Int
. Type : Retentio n Time : Unknow n N/ A ao7
. 10/26/201 0 J:25:5 5 P H Ve s 30. 0 14. 2 N o Manua l !4. 1 5an ^ Name : -b101025b128 " Sampl e ID : 'GLPICHJI-02-09049 ^ File : -b10102Sb.\M r Pea k Name : "PFHS * Mau(es) : "399.0/99. 0 amu.399.0/SO. O amu * Commen t 'DA L GW328 R 0 10092 8 {20X) - Annotation ; " Sampl e In < I'nk i N/ A 2.0e S 1.ge 5 1.8e 5 1.7e 5 1.6e 5 1.5« 5 1.4e 5 1.3e 5 1.2e5 - l.le S 1.0e S 9.0e 4 8.00 4 7.0e 4 6.0e 4 5.0e 4 4.0e 4 3.0e 4 Z0e 4 1.0e 4 14.1 0

, ,../V,.
. 1 6 1 8 

# I Sampl e Name : ^10102^)021 " Sampl e ID : "06008-194-10 * Fde : "b10102Sb.>M r Pea k Name : "PFOS " Mass(ea) : '499.0/99. 0 amu.499.CV80. 0 amu.499.0/130. 0 amt/ * Convnen t "5. 0 ng/m L F C s U i n M S Q V^ter ' /Annotation : Sampl e Index : 2 1 Sampl e Type : Standar d Concentration : 5,0 0 nq/m L Calculate dCone : 4,9 0 nq/(n L Acq
. Date : 10/25/201 0 Acq
. Time : 10:54:0 6 F H Sampl e Name:-b1O102SbO34 - San^t a ID:'LCS-101020-4 " Fle:-b101025b.wi r Pea k Name : TFOS " kAass(es} : "499.0/99. 0 a i Commen t *20pp b LCS " Annotation : - " Sampl e Index : 34 u,499.Dn30.0ami r

Nois e Perce n Ba.-e
. S'jb
. W Peak-split
. Rapor t Larg e Min
. Pea k

H e Min
. Pea k

W i Jraoothin g W

i R TWindow : Expecte dRT : Us e Relativ e Int
. Type : Retentio nT i Area : Height : En dTime : indo w

"act o s t P e ight : dtii : dtli :
RT : 5 0

l.O O c : 1 k : N o 0.0 0 0.0 0 0 30. 0 15. 1 N o Valle y 15. 0 1420B2 2 co u 3 31etO0 5 14. 9 15. 3 Acq
. Date : 10/2S/201 0 Acq
. Tima : 3:27:2 7 A K Modified : H o Proc
. Algcrithm : Int a1 1 iO'Ja n- Nois e Percentage : 5 0 Base
. Sub
. Window : 1.0 0 m Peak-Split
. Factor : 1 Repor t Larges t

Peak : N o MOI I
n
Hin
. Pea t

Height : 0.0 0 cp s I4in
. Pea k

width : 0.0 0 R TWindow : 50. 0 se c Expecte dRT : 15. 1 mi n Ua e Relativ e RT ; N o Int
. Type : Valle y Area : 4£3679 acount s Height : 1.05e+00 6 cp s En dTime : 15.: ' mi n e c p o m J

a I.OOe S 9.50e 5 9.00« 5 8.50e 5

7.00e 5 S.50e 5

5.00e 5 4.50e 5 4.00e 5 3.50e 5 3.00e 5 Z50e 5 2.00<. 5 1.50e 5 I.OOe S 5.00e 4 0.0 0 1 2 1 4 1 6 Printin g Time : 1:32:4 3 P M Printin g Date : Tuesday , Februar y 08 , 201 1 Pag e 4 o f 6 Pag e 4 7 o f8 3Buste r J293020 3 GLP10-01-02 ; Interi m Repor t 0 9 Bat^3|ym? f?ffi%2'5gH§ban d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0 

# I Sampl e Name : "b101025b045 - Sampl e ID : •LCS-101020-10 - Filo : -b1D1025b.wi r Pea k Name : 'PFOS ' Ma«s(es) : '499.0/99. 0 amu,499.0/80. 0 amu,499.0/130. 0 amu ' Commen t '20pp b LC S ECF " Annotation : " .•'ampl e Index : 4 5 Sampl e Type : y C Concentration : IS
. 6 ng/m L Calculate dCone : 21. 5 ng/m L Acq
. Date : 10/2€/201 0 Acq
. Ti l Modifie d R T Windo i Expecte d Us s Rela !

Height : Star t T En d Tim . 7:1S:0 4 A M 8.0e S

7.SeS 7.0e S 6.5e 5 6.0e 5 5.5e 5 5.0e 5 4.S«5

- 4.0e 5 3.Se 5 3.0e5 ^ 2.5e5

- 2.0e 5 

# I Sampt e Name : "b1O1025bO49 - Sampl e ID : "R O Wate rTN1(H)3i rFile : •b101025b.vw r Pea k Name : 'PFOS ' Mass{as) : '499.0/99. 0 anui,499.W80. 0 amu.499.0/130. 0 amu " Commen t "Metho d Blank " Annotation : ~ Sampl e Inde.»c : 49 Sampl e Type : Unknow n Concentration : N/ A Calculate dCone : - . 0 Acq
. Date : 10/26/201 0 Acq
. Time : e:4J:1 4 A M 170 0 Modified : Proc
. Algcr i Nx-:jis e Perce n hm : I n

age : Base
. Sub
. Window : P^ak-Split
. R^pur t Larg e Min
. Peak

H e Hin
. Peak

W i Smoothin g W

i R TWindow : E-pecte dRT : Us e Relativ e Int
. Type : Retentio nT i Area : Height : Star t Time : EndTime : Factor :

; t Peak ght : ith : ith : RT : V ne : 9 2 1. 7 N o telliQu a 5 0 1.0 0 1 N c 0. 0 0. 0 15. 1 N o alle y 15. 0 4 0 C O 5e.O0 3 15. 0 15. 1 

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- 1400

- 1300 1200

- 1100 1000

- 900

-



# I Sampl e Name : "b101025bOSO ' Sampl e ID : 'GLP1C>O1'D2-O&O01 - File : 'b101025b.Mif r Pea k Nama : "PFOS " Mass(es) : "499.0/99. 0 amu,499.0/80. 0 amu,499.0/130. 0 amu " Comment-DA L GW203 L 010092 9 (20X) " Annotation: -

•Type :

.e : 15. 0

1022255 4 Z

l 1.25et00 6 1.25e6 1.20e6 1.15e6 1. 1 Oe S 1.05e6 1.00e6 9.50e 5 9.00e 5 8.50e5 : aooe s 7.50e 5 g ^ 7.00e 5 ^ 6.50e 5 I6.00e 5

- 5.50e 5 5.00e 5 4.50e 5 4.00e 5 3.50e 5 3.00e 5 ZSOe S ZOOa S I.SOa S 1.00* 5 5.00e 4 0.00 

# I Sampl e Name : "bl01025b07 rSampl e ID : "GLP1OO1-02«»01 TFile : "b101025b.wi r Pea k Name : 'PFOS ' MaM{es) : '499.0/99. 0 amu,499.0/80. 0 amu,499.0/130. 0 amu " Commen t 'DA L GW222 R 0 10092 9 (50X) ' Annotation : " Sampl e Index : 7 1 Sampl e Type : I'nknown Concentration : N/ A Calculate dCone : 1130
. ng/m L Acq
. Date : 10/26/201 0 Acq
. Tima : 4:25:5 5 P M Modified : Ye s R TWindow : 30. 0 se c Expecte d RT : 15. 1 mi n
Int . Type : Manua l Retentio n Time : 15. 0 Area : 601606 1 co ^ Height : 7.JPevOO S 7.0e 5 6.5e 5 6.0e 5 5.5e 5 5.0e 5 4.5e 5 4.0* 5 3.5e 5 3.0e 5 Z5e 5 Z0e 5 1.5e 5 I.OeS

5.0e 4
0 0 15.0 2

14.87 ^



# , , , , I, , , Printin g Time : 1:32:4 3 PM Printin g Date : Tuesday , Februar y 08 , 201 1 Pag e 5 o f 6 Pag e 4 8 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Buste r J293020 3 Bat^P^lym? ^ ?f(ft%2'5§H§i?n d PFO S i n Groundwate r Quarterl yGroundwate rsamplin g - Sep t 201 0 

# r Sampl e Name : 'b101025b128 " Sampl e ID : 'GLPl(W)1-02-0&O49 ' File : •b101025b.'Mf r Pea k Name : 'PFOS ' llAass(es} : '499.0/99. 0 amu,499.0^0. 0 amu.4g9.0/130. 0 amu " Commen t -DA L G W328 R 0 10092 8 (20X rAnnotation : Sampl e Index : 12 9 Sampl e Tvpe : Unknow n Concentration : N/ A Calculate dCone : 171
. ng/m L Acq
. Date : 10/27/201 0 Acq
. Time : 12:25:4 2 P M Modified : Ye s R TWindow : 30. 0 se c Expecte dRT : 15. 1 min Us e Relativ e RT : K o Int
. Type : Manual Retentio nTime : 15. 0 min Area : 241953 9 count s Heiijht : 2.79e*00 5 cp s EndTime : 15. 2 mi n i

! • 1 Z7e5

- Z6e5

- 2.5e5

- Z4e5

- 2.3e5

- Z2oS

2. 1 e S Z0e5

- 1.9e5 1.8e5

- 1.7e5

- 1.6e5 I.SeS

1.4e5

- 1.3e5

- 1.2e5 l.la S I.Oe S 9.0e4 ' 8.0e4

- 7.0e4 6.0e4

- 5.0e4

- 4.0e4

- 3.0e4 2.0e4 1.0e 4

Printin g Time : 1:32:4 4 PM Printin g Date : Tuesday , Februar y 08 , Pag e 6 o f 6 201 1 Pag e 4 9 o f8 3Buste r

J293020 3 GLP10-01-02 ; Interi m Repor t 0 9 Bat'&RalymP f?m%2?§H§i^" d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0
b101025b.rd b (PFBS) : "Quadratic " Regressio n (" 1 /x " weigliting) : y= -1.43e+00 3 x* 2 + 3.85e+00 5 x+ 2.51e+00 4 ( r= 0.9995 )
2.5e7 - 2.4e7 - 2.3e7 - 2.2e7 - 2.187 - 2.0e7 - 1.9e7 - 1.8e7 - 1.7e7 - 1.6e7 - 1.5e7 - 1.4e7 - 1.3e7 - 1.2e7 - 1.1e7 - 1.0e7 - 9.0e6 - 8.0e6 - 7.0e6 - 6.0e6 - 5.0e6 - 4.0e6 - 3.0e6 - 2.0e6 - 1.0e6 - 0.0 - m. 

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0 5 1 0 1 5 2 0 2 5 3 0 3 5 4 0 4 5 5 0 5 5 Concentration , ng/m L 6 0 6 5 7 0 7 5 8 0 8 5 9 0 9 5 10 0 Printin g T i Printin g D a me : 1:33:0 2 P M

te : Tuesday , Februar y08 , 201 1 Pag e 1 o f 1 Pag e 5 0 of 8 3Buste r

J293020 3 GLP10-01-02 ; Interi m Repor t 0 9 Bat^P^lym? * ?m%2?§H4#" d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0 b101025b.rd b (PFHS) : "Quadratic " Regressio n(" 1 / x " weighting) : y = -1.36e+00 3 x'^ 2 + 3.35e+00 5 x + 9.93e+00 3 (r = 0.9997 ) 2.1e 7 2.0e7

- 1.9e7

- 1.8e7

- 1.7e 7 1.6e 7 1.5e 7 1.4e 7 1.3e 7 1.2e 7 1.1e 7 1.0e 7 9.0e6

- S.OeS

7.0e 6 6.0e 6 5.0e6- | 4.0e 6 3.0e 6 2.0e 6 1.0e 6 0. 0 8 0 8 5 9 0 9 5 10 0

1 0 1 5 2 0 2 5 3 0 3 5 4 0 4 5 5 0 5 5 6 0 Concentration , ng/m L 6 5 7 0 7 5 Printin g Time : 1:33:1 8 P M Printin g Date : Tuesday , Februar y08 , 201 1 Pag e 1 o f 1 Pag e 5 1 o f 8 3" * Buste r

J293020 3 GLPIO-01-02 ; Interi m Repor t 0 9 Bat^Ra!ym? fem^^lEHa^an d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0
b101025b.rd b (PFOS) : "Quadratic " Regressio n (" 1 / x " weighting) : y= -1.03e+00 3 x'^ 2 + 2.89e+00 5 x+ 3.08e+00 4 ( r= 0.9997 )
1.9e 7 1.8e 7 1.7e 7 1.6e7 ^ 1.5e 7 1.4e7 H 1.3e 7 1.2e 7 1.1e 7 1.0e 7 g 9.0e 6 8.0e6

- 7.0e6

- 6.0e6

- 5.0e 6 4.0e 6 3.0e6

- 2.0e6

- 1.0e 6 0. 0 

# y /

• , / / /

, ^

1 0 1 5 2 0 2 5 3 0 3 5 4 0 4 5 5 0 5 5 6 0 Concentration , ng/m L 6 5 7 0 7 5 8 0 8 5 9 0 9 5 10 0 Printin g Time : 1:33:3 3 P M Printin g Date : Tuesday , Februar y08 , 201 1 Pag e 1 o f 1 Pag e 5 2 of 8 3Buste r

J293020 3 GLP10-01-02 ; Interi m Repor t 0 9 Bat^Ra|ym? f^fM-olsEt^cat^n d PI'O S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0

I Sampl e Name:'b101108b026 ' Sampl e ID : I Peal cName : "PFSS " Masses) : "299.0/99. 0 •Commen t "2. 5 nflftn L F C sl d i n Mil l Q Water " Sampl e Index : 2 6 Sampl e Type ; Standar d Concentration : 2.S O ng/m L Calculate d Ccnc : 2.6 0 nq/n L Acq
. Date : 11/9/201 0 Acq
. Time : 10:15:1 6 P M

Min
. Pea k

Hidt h Fraoothin g Viidt h R TWindow : Expecte dRT : Us e Relativ e R

T Int
. Type : Retentio nTime : Area : 1 Height : Star t Time : En dTim^ : 9 2 0.0 0 0 30. 0 12. 5 N o Valle y 12. 6 .03 3 c 71e+00 5 12. 5 12. 5 "08008-194-9 " File : "b101108b.wi r rui,299.0/8O. 0 amu ' /Annotation: "
2.7* 5 2.6e S 2.5e 5 2.4a 5 2.3e 5 2.2e 5 2.1e 5 2.0e S 1.9e 5 I.Be S 1.7e 5 1.6e 5 1.5e 5 1.4e 5 1.3a5 ' 1.2o 5 l.le S 1.0e 5 9.0e 4 8.0e 4 7.De 4 6.0e 4 5.0e 4 4.0e 4 3.0e 4 2.0e 4 1.0e 4 

### • Sampl e Name : 'blO l 108b033 - Sampl e ID : 'R O Wata rTN10-03i rFile : 'blOHOSb.wi T Peal cName : "PFBS - Mass(es) : *299.0/99. 0 amu.299.W80. 0 amu " Commen t -Metho d Blanl ^ Annotation: " Sampl e Index : 3 2 Sampl e Type : Unknov n Concentration : Acq
. Date : Acq
. Time : ModiEiad : Proc
. Algorithm : Hin
. Paa k

Haigh i Smoothin g Width : R TWindow : Expecte dRT : Us e Relativ e RT : int
. Type : Retentio nTime : Heigtit : Star t Time : En dTime : M/A 11/9/201 0 12:^2:3 5 A

M N o Int^lliQua n- IQ A : O.D O cp s 0 point s 30. 0 se c 12. 6 mi n• H o Valle y llSi O count s 3.52e»00 3 cp s 12. 5 mi n 12.- J mi n i

. : 340 0

280 0 260 0 240 0 220 0 200 0

160 0 140 0 120 0 100 0 

# ( Sampl e Name : -b101108b036 " Sampl e ID : " Peal i Name : 'PFBS * Uass(es) : "299.0/99. 0 a i Commen t "2pp b LCS ' Annotation : " • Sampl e Index : 3 6 Sampl e Tvpe : Concsntcation : Calculate dCone : Acq
. Date : Acq
. Time : Modified : Proc
. Algorithm : Min

- Pea k

Heigh t Min
. Pea k

Hidth : Smoothin g wi.ith : R TWindow : E.tpecta dRT : Us e Relativ e RT : Int
. Type : Retentio nTime : g c 2.0 0 2.3 5 ll/9/20l ( ng/m L

ng/m L 1: 4 5:4 4 A

H N o IntelliO u 0.0 0 0.0 0 0 30. 0 12. S N o Valle y 12. 6 Area : 109414 2 c o Height : 2 Star t Time : En dTime : .52e+0O S 12. 5 12. 9 a n- I S

c p s point s s e c mi n

unt s mi n mi n 2.5e 5

2.4e S 2.3e 5 2.2e 5 2. 1 e S 2.0e 5 I.Se S I.ee S 1.7e 5 1.6e 5 1.5e 5 1.4e 5 1.3e 5 1.2e S l.le S 1.0e 5 9.0e 4 8.0e 4 7.0e 4 6.0e 4 S.0e 4 4.0tt 4 3.0e 4 2.0e 4 1.0e 4 0. 0 

# I Sampt e Name:-b101108bO4 rSampl e ID : "LCS-101020-7 * File : "blOIIOSb.wiff Pea k Name : "PFBS " Mass(es) : "299.0/99. 0 amu.299.0/80. 0 amu " Commen t *^p b LC S ECF " Annotation : " " Sampl e Index : AI Sampl e Type : Q C Concentration : 0.0 0 ng/m L Calculate dCone : • ' 0 Acq
. Date : 11/9/201 0 Acq
. Time : 4 :13:1 4 A M Modifi i H o Proc
. Algcrithm : Min
. Pea k

Heigh t Hin
. Pea k

Width : Sisoothin g Width : R Twindow : E^:pect9 dRT : Us e Relativ e RT : Int
. Type : Retentio nTime : Area : Height : Intell i : 0.0 0 0.0 0 0 30.1. 1 12. 6 N o Valle \ 12. 6 1J55 9 ;.99e*0 0

Dat a printe d byST W Printin g Time : 12:29:1 1 P M Printin g Date : Tuesday , Marc h22 , 201 1 Pag e 1 o f 4 Pag e 5 3 of8 3Buste r J293020 3 GLP10-01-02 ; Interi m Repor t 0 9 Bat^Ra!y|fe? f?f(ft?-o'8EHc?^#n d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0 

# I Sampl e Name : -b101108b070 ' Sampt e ID:"GLP10O1-02-0&<l3 rFile : -blOIIOBb.wi r Pea k Name : 'PFBS ' MasK(es) : -299.0/99. 0 amu,299.0/80. 0 anu T Commont"DALGW317L0100924 ' /Annotation : " ampl e Index : 7 0 ampl e Type ; N/A Calculate dCone : 0.039 2 ng/m L Acq
. Date : 11/9/201 0 Acq
. Time : 1:41:5 1 P H Algorithm : Intellit'ua n- : ea k Height : 0.0 0 cp s ea K Width : 0.0 0 se c 1.4 c

R T Window : Expecte dRT : Us e Relativ e R

T Int
. Type : Retentio nTime : Area : Height : Star t Time : En dTime : 30. 0 12. 5 N o Valle y 12. 6 6132 2 c 1.39e+00 4 12. 5 12. 7 I " 7000. 0
6000.0

- 5000. 0

8.6 6 iL ...I t •» ,i
. eA^ ah>Jtjlli I

h< 11 1 J I ftiwl 

# I Sampl e Name ; "bl01108b026 " Sampl e ID : "08008.194-9 " File : "b101108b.wii r Pea k Name : "PFHS " Mass(as) : "399.0/99. 0 amu,399.0/80. 0 amu " Commen t "2. 5 ng/m L F C st d i n Mill i Q Water " ampl e Index : 2 6 ampl e Type : Standar d Concentratio n Calculate dC

o Acq
. Daca : Acq
. Time : Kodified : Proc
. Algoci t Hin
. Peak

Hei Hin
. Peak

Wi c m : q h t t h : Smoothin g Width ; R T Window : Expecte dRT : 2.5 0 2.5 6 ll/q/20 ! 10:15:1 9 H o ng/m

L ng/m L i P M Intelli0.ja n- IQ 0.0 0 0.0 0 0 30. 0 14. 2 c p s point s mi n

Area : Height : Stsr t Time : EndTime : 10:?931 0 2.5 2 = 1 I

Sampl e Index : Sampl e Tvpe : Concentration : Calculate d Con e Acq
. Date : Acq
. Tima : 3 3 Unknow n N/A < 0 ll/S/201 0 12:42:3 5 A H Modified : K o Proc
. Algorithm : IntelllOua n - IQ A Min
. Peak

Height ; 0.0 0 cp s Min , Peak

Width : 0.0 0 se c Smoothin g Width : 0 point s R T Window ; 30. 0 se c E.tpecte dRT : 14. 2 ntin Us e Relativ e RT : N o 

# I Sampl e Name : "b101108b033 ' Sampl e ID : "R O WatsrTN1(M)3i rFile ; "blOllOab.wif r Pea k Name : "PFHS " Massfes) : "399.0/99. O amu,399.0/80. 0 amu " Commen t "Metho d Blanl ^ Annotation : * *
340 0 320 0 3000 - 280 0 260 0 2400 - 2200 - 200 0 1800 - 1600 - 140 0 120 0 100 0 80 0 I Sampl e Name : "blO l 108b03 rSampt a ID : 'LCS-101020-2 " File : "blOllDSb.wi r Pea k Name : 'PFHS " Mass{es) ; "399.0/99. 0 amu.399.a/80. 0 amu " I Commen t "2pp b LCS " Annotation ; " *

Int . Type : Retentio n Area : Height : Star t Tim . En d Time : Valle y 5J5 & count s 1.266*00 3 cp s 14. 2 mi n Sampl e Type : Concenrratio i Calculate dC

o Acq
. Datrt : Acq
. TifUfl

J Q

C 2.0 0 ng/m

L 2.3 7 ng/m

L 11/9/201 0 1:45:4 4 ftM

Smoothin g width : R T Kindo-J : Expecte d RT :

iiiL,i..jii|tai,mt<i i 2.3e 5 2.2e 5 2. 1 e 5 Z0e 5 1.9e 5 I.Be S 1.7e S I.ee S I.SeS ' 1.4e S 1.3e S 1.2e 5 l.le S l.Oe S 9.0e 4 8.0e4 - 7.0e 4 6.0e 4 S.0e 4 4.0e 4 3.0e 4 Z0e 4 1.0e 4 G O 1 4 3 3



# i 16.30.^;,,^16,S018.4 5 1 2 1 4 1 6 I S 2 0 Dat aprinte db y ST W Printin g Time : 12:29:1 2 P M Printin g Date : Tuesday , Marc h22 , 201 1 Pag e 2 o f 4 Pag e 5 4 o f8 3- * Buste r

J293020 3 GLP10-01-02 ; Interi m Repor t 0 9 Bat^Ra|ym? f?ffl?'o'8§H<Si?" d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0



### • Sampl e Name : 'b10l108b04 rSampl e ID : 'LCS-101020-7 " File : -blOIIOBb.wi r Pea k Name : "PFHS " Masses) : "399.0/99. 0 amu,399.0/80. 0 amu " Commen t -2pp b LC S ECF " Annotition : ~ Sampl e Typ e Concentrati < QC 0.0 0 0,10 0 11/9/201 0 4:13:1 4 A

M
thm : IntelliQua i •Sampl a Name;-'b1011O8bO70 " Sampl e ID:'GLPiabl-02-09O37 " File;"b101108b.wif r I Pea k Name : "PFHS " Mass(es) ; "399.0/99. 0 amu.399.0/80. 0 ami T •Comment-DALGW317L0100924 " Annotation: - Sampl e Index : 7 0

R T Wi r Expec i ea k Height ; ea k Width : in g Width :

Valle y

4976 5 c 1.00e 4 9500.0 0 gooo. w 8500.0 0 8000.0 0 7500.0 0 7000.0 0 6500.0 0 6000.00

-

"
. 5500.0 0 I5000.0 0

" 4500.0 0 4000.0 0 3500.00

- 3000.0 0 2500
. W 2000.00 - 1500.0 0 1000.0 0 500.0 0 0.00 ' ]
. . , _1 - L-Y "
. . ....f^*fa-ji..*.-J[fi ^ Sampl e Type : Concentration ; Calculate dCone : Ac4
. Date : Acq
. Time : Modified : Proc
. Algcrithm : Hin
. Pea k

Heigh t Hin
. Pea k

Width : Smoothin g Width : R TWindow ; Expecte dRT : Us e Relativ e RT : I'nknov n H/ A 0.23 6 n g U/9/201 0 1:41:5 1 P

M N o IntelliQ'ia n- : 0.0 0 cp s 0.0 0 se c 0 poin t 30. 0 se c 14. 2 mi n H o Int
. Type : Vaile y Retentio nTime : 14. 3 mi n Area : 10393 7 count s H-iight : 2.37ei00 4 cp s Star t Time : 14. 0 mi n En dTime : m L

IQ A



### 1 

## 1 Z3e 4 2.2e 4 Zle 4 2.0e 4 1.9e 4 1.8e 4 1.7e 4 1.6e 4 1.5e 4 1.4e 4 1.3e 4 1.2e 4 1. 1 e 4 1.0» 4 9000. 0 8000. 0 7000. 0 6000. 0 50OO. 0 4000. 0 3000. 0 20OO. 0 1000. 0

I SampleNan>e:-bl01106b026 ' Pea k Name:-PFOS - Mass<efi) : I Commen t -2. 5 nglM
. F C st d i n h
Concentrati o Calculate dC Acq
. Date ; Acq
. Time : Modified : Proc
. Algor i Nsis e Perce n Ba:-e
. Sub
. W : 2.5 0

n c : 2.5 1 ll/a/201 0 nc)/m L

ng/m L 10:15:i e P

H N o hm : IntelliQ u age : S O ndow : 1,0 0 Peak-Split
. Factor : 1 Repor t targ e Min
. Pea k

H e t Peak : N c n- M Q mi n ght : 0.0 0 cp s Min
. Pea k

Width : 0.0 0 se c Smoothin g Width : 0 R TWindow : Expecte dRT : Us e Relativ e Int
. Type : Retentio nT i Area ; Height : ?tar t Time : En dTime : 30. 0 15- 4 RT : H o Valle y e : 15. 4 96338 2 C

O 2.516+00 5 15. 3 15. S p o

s e c mi n

mi n n t s c p s mi n Sampl e ID : -08006-194-a ^ -499.0/99. 0 anw.499.Q/80. 0 a i n a Q Water " /Vmotation:" " iSeS Z4e5 - Z3e 5 Z2e 5 Z1e5 - 2.0e5 - 1.9o5 - 1.ae5 - 1.7e5 - I.SeS 1.5e5 - 1.4e 5 1.3e 5 1.2«5 - l.leS l.Oe S 9.0e 4 S.0e4 ' 7.0e 4 6.0e 4 5.De 4 4.06 4 3.0» 4 Z0» 4 1.0e4 ' 0. 0 

# I Sampl e Name;-b10110SbO33 " Sampt e 10 : "ROWMerTNICHQi rFiie:"bl01108b.wi r Pea k Name : 'PFOS " Massfes) : "499.0/99. 0 amu.499.(V80. 0 amu.499.(V130. 0 ami T Commen t "Metiyi d Btanl TAnnotation : * " Sampl e Index : 33 Sampl e Type : I'nknow n Concentration : N/ A Calculate d Cone : 0.012 5 ng/m L Acq
. Date : 11/9/201 0

R^po i t Larges t Pe ; Pea k Height : Hin
. Pea k

Width : Smoothin g width : R TWindow ; Expecte dRT : Us e Relati

- RT :

2153 1 =a i 4
. S6e*00 3 15. 2 15. 5

1 4 1 6 1 8 Dat aprinte db y ST

W Printin g Time : 12:29:1 2 P M Printin g Date : Tuesday , Marc h22 , Pag e 3 o f 4 201 1 Pag e 5 5 o f8 3Buste r

J293020 3 GLPIO-01-02 ; Interi m Repor t 0 9 Bat'^Ra!ym? f?m?'olgt^c&t?" d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0 

# I San ^

Name : "b101108b03 rSampl e ID : 'LCS-101020- r Pea k Name : 'PFOS " Macs(as} : "499.099. 0 amu,499.0/80. 0 a : Commen t "2pp b LCS " Annotation: " Sampl e Type : QC

ellitua i B O 1,0 0 Peak-Split
. Facto r Repor t Larges t

Pe a Hin , Pea k

Height : Hin
. Pea k

Hidth : Smoothin g Hidth : R TWindow : Expecte dRT : Us e Relativ e RT : Int
. Type : Retentio nTime : Area ; S5 1 Height ; 2
. Star t Time : En dTime : 1

. : H o 0
. 0
. 0 30. 0 15. 4 N o 'alle y 15. 4 7 3 c 4e+00 5 15, 2 15. 6 2,2* 5 Z1e 5 ZOe S 1.9e 5 1.8e 5 1.7e 5 1.6e 5 1.5e 5 1.4e 5 1.3e 5 1.2e 5 1.1. 5 I.Oe S 9.0e 4 S.0e 4 7.0e4

- 6.0* 4 5.0e 4 4.0e 4 3.0e 4 Z0e 4 1.0* 4 153 5

15.2 U iil ^ 18.4 5 

### • Sampl e Name : -b101108b04 rSampl e ID : -LCS-101020- rFile : -blOIIOSb.wrt T Pea k Name : 'PFOS - Man<as} : -499.0/99. 0 amu,499.0/80. 0 amu,499.Q n 30. 0 amu ' Commen t -2pp b LC S ECF " Annotation ; ~ Sampl e Index : 4 3 Sampl e Type : Q C Calculate dCon e Acq
. Date : Acq
. Time : Modified : B TWindo-.v : Expecte dRT : Us e Relativ e R

T Int
. Type ; 1.5 8 ng/m L 2.1 4 ng/m L 11/9/201 0 4:13:1 4 A

M Ye s 30. 0 se c 15. 4 mi n N o Manua l 15, 3 mi n Area : S30S6 9 count s Height : 1.42e'-00 5 cp s Star t Time : En dTime ; 14. 5 mi n 15. 5 tni n 

### & t 

## 1 1.4* 5
1.3e S
1.2e 5
1.1* 5
1.0* 5
9.0e 4
S.0e 4
7.0e 4
6.0e 4
5.0e 4
4.0e 4
3.0* 4 

# I Sampl e Name : -b1O1lO6bO70 ' Sampl e ID ; -GLP1(H)1-02-0»<l37 - File ; "blOIIOBb.wi r Pea k Nama : "PFOS " Mats(es) : '499.0/99. O amu.499.(V80. 0 amu,499.0/130. 0 amu " Commen t "D/U.GW317 L 0 100924 " /Annotation : "
Z7e 4 ZSe 4 Z5e 4 Z4e 4 2.3e 4 Z2e 4 Z1e 4 Z0e 4 1.9e 4 1.8e 4 1.7e 4 1.6e4 - 1.5* 4 Sampl e Type : Concentration : Calculate dCo n Acq
. Date : Acci
. Time ; Modified : R TWindow : Expecte dRT : U nknow n K/ A : : 0.41 5 11/9/2 0 1:41:5 1 Ye a 30, 0 15. 4 0 PI 1

1.4* 4

1.3e 4 1.2e 4 1.1e 4 1.0e4 - 9000. 0 8000. 0 7000.0 - 6000.0 - 5000. 0 4000. 0 3000. 0 2000. 0 1000.0 - 0. 0 

# LJK ^
1 0 1 2 1 4 1 6 1 8 2 0 Dat aprinte db y ST

W Printin g Time : 12:29:1 3 P M Printin g Date : Tuesday , Marc h22 , 201 1 Pag e 4 o f4 Pag e 5 6 o f8 3Buste r

J293020 3 GLPIO-01-02 ; Interi m Repor t 0 9 Bat^R^lyiJ^p fffftq,^§H^^j^n d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0

•blO l 108b.rd b (PFBS) : "Quadratic " Regressio n (" 1 /x " weighting) ; y= -2.33e-H00 3 x'^ 2 -i - 4.57e-H00 5 x+ 2.06e-^00 4 ( r= 0.9998 ) 1.7e 7 1.7e7 - 1.6e7 - 1.5e7 - 1.4e7 - 1.3e7 - 1.2e7 - 1.1e7 - 1.0e7 - i 9.0e6 - 

### 8 S 8.0e6 - 70e6 - 6.0e6 - 5.0e6 - 4.0e6 - 3.0e6 - 2.0e6 - 1.0e6 - 0.0 - 

### ,-'' ' ---' //'" '

y


### , /


# X

0 2 4 6 8 1 0 1 2 1 4 1 6 1 8 2 0 2 2 2 4 2 6 2 8 3 0 3 2 Concentration , ng/m L 

### .--^ ' ^ y y

3 4 3 6 

### ^^- ^

3 8 /-^'• '

4 0 y ^ '

4 2 4 4 

### ,/-' "

4 6 

### ,

4 8 

### ,-'•'•• '

5 0
Dat a print e Printin g T i Printin g D a d byST W

me : 12:26:2 5 P M te : Tuesday , Marc h22 , 201 1 Pag e 1 o f 1 Pag e 5 7 o f 8 3** • Buste r

J293020 3 GLPIO-01-02 ; Interi m Repor t 0 9 Bat^R^lyiJ^p fffftq,olg§HS^^an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sept 201 0

•blOllOSb.rd b (PFHS) : "Quadratic " Regressio n (" 1 /x " weighting) : y= -2.12e-H00 3 x'^ 2 + 4.05e-K00 5 x+ 8.31e-K00 3 ( r= 0.9997 )
1.5e7 - 1.4e7 - 1.3e7 - 1.2e7 - 1.1e7 - 1.0e7 - 9.0e6 -
c8.0e6 - 

### 8 2 7.0e6 - 

### < 6.0e6 - 5.0e6 - 4.0e6 - 3.0e6 - 2.0e6 - 1.0e6 - 0.0 - 

### •,/-'" ' 

# X' "

y ^ 

# X / " - y

y 

### / >

0 2 4 6 8 1 0 1 2 1 4 1 6 1 8 2 0 2 2 2 4 2 6 2 8 3 0 3 2 3 4 Concentration , ng/m L f - 7 '• • 3 6 yy- ^

3 8 

### ,_- -

4 0 y-^'' '

- > 1 4 2 

### ^,..-' '

4 4 

### ,^'-" '

4 6 

### ^

7 4 8 y - ^

•^—I — 5 0 Dat aprinte db y ST

W Printin g Time : 12:26:4 6 P M Printin g Date : Tuesday , Marc h22 , 201 1 Pag e 1 o f 1 Pag e 5 8 o f8 3Buste r

J293020 3 GLPIO-01-02 ; Interi m Repor t 0 9 Bat^Ra|ym? fef(ft?.ol8iH&ba" d PI'O S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0

•bl0 1 lOSb.rd b (PFOS) : "Quadratic " Regressio n (" 1 /x " weighting) : y= -1.83e-H00 3 x'^ 2 + 3.84e-H00 5 x+ 1.66e-^00 4 ( r= 0.9997 ) 1.5e 7 1.4e7 - 1.3e7 - 1.2e7 - 1.1e7 - 1.0e7 - 9.0e6 - 4 2 8.0e6 - c 

### 8 C D 7.0e6 - 

### < 6.0e6 - 5.0e6 - 4.0e6 - 3.0e6 - 2.0e6 - 1.0e6 - 0.0 - 

# y ^ / ' / •/-'"' ' ,/' '

y y y y '



## y ^ 

# x ' y
0 2 4 6 8 1 0 1 2 1 4 1 6 1 8 2 0 2 2 2 4 2 6 2 8 3 0 3 2 3 4 Concentration , ng/m L y ' '

1 3 6 .•••-'•' '

3 8 y ' ^

4 0 

### . - -

1 4 2 

### ' ^

" •1 1 4 4 1 4 6 

# y4 8 ,.-•# •

5 0
Dat a printe d b

yST W Printin g Time : 12:27:0 5 P M Printin g Date : Tuesday , Marc h22 , 201 1 Pag e 1 o f 1 Pag e 5 9 o f 8 3Ginge r

AG0133050 9 GLPIO-01-02 ; Interi m Repor t 0 9 Res^Pfl'^StfFa^.i^fi<aS..m d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0 

# I Sampl e Name : 'g110211a022 - Sampl e ID : '10007-27-11 ' File ; 'g110211a.wi r Pea k Name ; 'PFBS " MaM<ea) : "299.0/99. 0 amu.299.0/80. 0 amt T Commen t *1 0 ng/m L F C st d i n Mi S Q water ' Anrwbtion ; Sampl e Index : 2 2 Sampl e Type : Standar d Concentration : 10. 0 nq/n L Calculate d Cone : 9.7 5 ng/m L Acq
. Date : i/12/201 1 Acq
. Time : ixlAib AM A Sampt e Nanne : -911021 1 a026 " Sampt e ID : 'Solvan t Btanl T Pea k Name:-PFBS * Ma»«(e«):'299.0ra9.0amu.299.CV80. 0 Commen t 'Mill t Q \AMe rTNII-OOS r/Annotation : " Sampl e Index : 2 9 Sampl e Type : I'nknown Concentration : H/ A Calculate dCone : - . 0 Acq
. Date : 2/12/201 1 Acq , Time : ^:02:5 1 A M Modified : R TWindow : Expecte dRT : us e Relativ e int
. Type : Retentio nT i Area : Haight : Star t Time : En dTime : R

T

i Ve
. 3 0
. 1 0 N o llanu 1 0 9739 7 1J20CI 0 1 0 n 6
1 a
s J Modified : Proc
. Alg^-rithm : Nois e Percentage : Base
. Sub
. Hindo o PeaK-Split
. Fact o N o

nt^lliQua n S O 1.0 0 : 2 Rapor t Larges t

Paak : N

o Hin
. Peak

Haight : Hin
. Pea k

Width : Smoothin g Width : R TWindow : Expecte dRT : I's e Relativ e RT : Int
. Type ; Retentio nTime : Area : Height : Star t Time : EndTime : 0.0 0 0.0 0 - HQI

I mi n
c p s poi n ?0. 0 3e c 10. 6 m M o valle y 10. 6 m e0 3 =ou

n US
. cp: : 10. S m 10. 7 m n " " s

s . 60 0



# I Sampl e Name : 'g110211aO3 rSampl e ID ; 'LCS-11021&« - File ; 'gl10211a.«wf r Pea k Name ; -pFBS " Uass<es) : "299.0/99. 0 amu,299.0:180. 0 amu " Commen t 'Spp b LCS " Annotation ; ~ Sampl e Index : 3 3 Sampl e Type : g C Ccncentra t Calculate d Acq
. Date ; Acq
. Time : Modified : R TWindow : Expecte dR

I o n : Con e 4.9 B ng/m L : 5.5 2 ng/m

L 2/12/201 1 6:32:5 7 A

H Ye 3 30. 0 se c 10. S rain
Hcig.'-.r ; Star t Tim i EndTim- : s



### f 8.0e 5 7.5e 5 7.De 5 e.Se S e.0e S 5.5e 5 5.0e 5 4.5e 5 4.0e 5

2.0e 5 1.5e5 I.OeS 5.0e4

- Sampt o Name ; "g110211a042 - Sampl e ID ; "LCS-110210-14 " File ; 'gl102na.wif r Pea k Name ; 'PFBS - Mass(es) ; -299.CV99. 0 amu.299.0m0. 0 amu Commen t "Spp b LC S ECF " Annotation : " Sampl e Index : 42 Sampl e Type : Q C 0.0 0 ng/m

L 0.057 8 ng/m

L Acq , Date : 2/12/201 1 Acq
. Tima : 9:14:5 9 A M

Dat aprinte db y ST

W Printin g Time : 12:33:4 1 P M Printin g Date : Tuesday , Marc h22 , 201 1 Pag e 1 o f 4 Pag e 6 0 o f8 3* * Ginge r AG0133050 9 GLPIO-01-02 ; Interi m Repor t 0 9 Res^P?&'%SAPF^?'cg f(['aS.M ' P^O S i n Groundwate r Quarteri yGroundwate rSamplin g - Sept 201 0

•Sampt e Name : •fl110211a114 " Sampt e ID ; •GLP1(M)1.02-09-025 ' FBe : •gl10211a.wi r 1 Pea k Name:-PFBS ' Has«{es) : *299.0«9. 0 amu,299.0O0. 0 amu " •Comment'DALGWGRS040100930{diIl;500 rAnnotation ; " Sampl e Indaj; : 114 Sampl e Type : Unknow n Concenrrjtion : N/ A Calculate d Cone ; 2360
. ng/m L fiSeSAcq
. Dat« : 2/13/201 1 Acq
. Tima : 6:52:^ 3 A H Modified : Ye s ®-°» ^ Proc
. Algorithm : IntelliCua n - MGI I Nsis e Percentage : 5 0 Bare
. Sub
. Windcw : 1.0 0 mi n 5.5e5 - PeaK-Split , Factor : 3 Repor t Latyea t Peak ; H o Min
. Pea k

Height : 0-0 0 cp s 5,0e 5 Min
. Pea k

Width : 0.0 0 se c Smoothin g Kidth : 3 point s R TWindow : 30. 0 se c4.Se 5 Expecte d RT : 10, S mi n Us e Relativ e RT : N o 4.0e 5 Int
. Type : Bas e T o Bss e Retentio n Time : 10. S mi n - AL-ea : 317274 7 count s & Height : 670000
. -p s ^ •'•^= * Star t Time : 10. 2 mi n ^ En d Time : 11. 3 n,i n s £lOe S ZSe S
2.0e S
1.5e 5
1.0e 5
5.0e 4

1 2 3 4 5

•Sampt e Name : "Bl102na02 rSampl e ID ; "Solven t Blank ' File ; "g1102 1 la.wi T 1 Pea k Name ; "PFHS " Mass(e*):-399.Qre9.0amu,399.0raO.OamL r •Comment'MilBQWatsrTNU-OOS rAnnotation; - Sampl e Index : 2 9 Sampl e Type : Vnkno-J n Cancentration : N/ A Calculate d Cone : < 0 *•'" " Acq
. Data : 2/12/201 1 Acq
. Time : 5:02:5 1 A M ^°° ° Modified : N o 380 0 Proc
. Algorithm ; IntelliQua n - MQI l Nois e Percentage : 5 0 360 0 Ba.-e
. Sub , Window : 1.0 0 mi n Peak-Split
. Factor : 2 ^00 - Repot t Larges t Peak : H- ; Min
. Pea k

Height : 0.0 0 cp s 3200 - Min
. Pea k

Hidth : 0.0 0 sa c Smootliin g Width : 3 point s 300 0 R TWindow : 30. 0 seer . Expecte d RT : 11. 7 mi n 280 0 Us s Relativ e RT : N o 260 0 Int
. Type : Valle y Retentio n Time : 11. 7 mi n . 240 0 Area : 153 9 count s fr Haight : 332
. cp s ^ 220 0 Star t Time : 11. 5 mi n " B Eri d Time : 11. B mi n | 200 0 180 0 160 0 140 0 120 0 100 0 ao o 60 0 40 0 20 0 

### 1 **iJw - ..... J A
. ^ -i... - . M 1 2 3 4 5 10.5 9

1D.4s J


### , ' ^ i 7 8 9 1 0

Time , mi n

, . i l S 7 8 9 1 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7



### j 

# l l y W F i M 

# Ai i\ m



# . r ^ lyj r Mf\* ^ 

### /

f1 

# f i J W ^ V 1 1 1 2 1 3 1 4 1 5 1 6 1 7 •Sampt e Name:-Bll0211a02 rSanpt e ID : "10007-27-11 " Fito ; "g110211a.wrt r I Pea k Name : "PFHS " Wa»«(e*) ; "399.0ra9. 0 amu.399.Qreo. O amu " Sampl e Index : 2 2 Sampl e Type : Standar d Concentration : 10. 0 ng/m L Calculate d Cone : 9.8 3 ng/m L -_ - Acq
. Date : 2/12/201 1 ^'^' ^ Acq
. Time : 3:14:5 J A M 2.4a6 - Hodified : U o 2.3e6 - Proc - Algorithm : IntelliQua n - MQI I _, . Nois e Percentage : 5 0 ^-^ ^ Base
. Sub
. Window : 1.0 0 mi n 21e6 - P-!ak-3plic
. Factor : 2 R^p^r t Larges t PeaK : N = Z0e 6 Hin
. Pea k

Height : 0.0 0 cp s Hin
. Pea k

Width : 0.0 0 se c^3t> S Smoothin g Width : 3 point s l.Se6 ' R TWindow : 30. 0 se c E.-pecte d RT : 11. 7 mi n 1.7e6 - Us e Relativ e BT : N o Int
. Type : Valle yi_5e6
. Retentio n Time : 11. 7 mi n . Area : 1132773 5 count s g " 1.4e6 ' Height : 2600000
. cp s * ,, ^ Star t Time : 11. 5 mi n 9 ^-^ ^ En d Time : 12. 2 mi n J 1.2e6 ' 1.1e 6 1.0e 6 9.0e S B.0e 5 7.0e 5 6.0e 5 5.0o 5 4.0e 5 3.0e 5 2.0e 5 I.Oe S

°' ° 1 2 3 4 5 1 Sampt e Name:-a110211a03 rSampt e ID ; "LCS-11021t ^ File : "g110211a.v.i r I Pea k Name : "PFHS " Mass<es) ; "399.0/99. 0 amu.399.0/80. 0 amu " Sampl e Index : 3 3 Sampl e Type : O C Concentration : 4.9 S ng/m L Calculate d Cone : 5.3 6 ng/m L Acq
. Date : 2/12/201 1 1.4e 6 Acq
. Tima : 6:32:5 7 AM Hodified : N o 1.3e6 - Proc
. Algorithm ; IntelliQuar
. - HQI I Nois e Percentage : 5 0 Base
. Sub
. Window : 1.0 0 mi n 1.2e 6 Peak-Split
. Factor : 2 Repor t Larges t Peak : N o Min , Pea k

Haight ; 0.0 0 cp. ^ 1.1e 6 Hin
. Pea k

Width : 0.0 0 se c Smootliin yWiJtli : 3 point s R TWindow : 30. 0 se cl-Oe S Expecte d RT : 11. 7 mi n Us e Relativ e RT : N o 9.0e S Int
. Type ; Valle y Retentio n Time : 11. 7 mi n « Area : 638920 1 count s g " S.OeS Height : H70000
. cp s ^ Star t Time : 11. 5 rain a En d Time : 12. 2 mi n | '-O^ ^ 6.0e 5 S.0e 5 4.0e 5 3.0e 5 ZOe S I.Oe S
1 2 3 4 5 1 1 6 8

k
. 6 7 8 9 1 0 1 1 1 2 1 3 1 4

Tim e mi n

lie s

. L 6 7 8 9 1 0 1 1 1 2 1 3 1 4 1 S 1 6 1 7

1 5 1 6 1 7 Dat aprinte db y ST W Printin g Time : 12:33:4 2 P M Printin g Date : Tuesday , Marc h22 , Pag e 2 o f 4 201 1 Pag e 6 1 o f8 3Ginge r AG0133050 9 GLPIO-01-02 ; Interi m Repor t 0 9 Res^P?&'S^S!iPF^?.cg!i^aS..M ^ PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0

Pea k Kep o Min
. Min
. Smo o R T W Exp e -spl i r t L a Pea k Pea k hin g ste d t., e V J Fa c .•3 t Iq h d t h Widt h R T I Sampl e Name ; "gl102l1a042 - Sampt e ID;'LCS-110210-14 " Fae:-g110211a.<M r Pea k Name ; "PFHS " Mass(es) ; '399.0/99. 0 amu,399.0/80. 0 ami T I Convnen t "Spp b LC S £C P Annotation;" "

ao»4 7.5e4 ' 7.0e 4 6.5e 4 6.0e 4 S.Se 4 5.0e 4 4.5 M 4.0»4 - 3.5e 4 3.0e 4 2.5e 4 2.0» 4 1.Se4 - 1.0e4 - 5000.0 - Height : Star t Ti n En dTime ;

0. 0 

# I Sampt e Name ; "g110211a114 - Sampt e ID ; "GLPl(W)1-02-09O25 " Fito ; 'g110211a.wt r Pea k Name ; 'PFHS * Man(es) : '39g.0i«9. 0 amu,399.(V80. 0 amu ' Commen t 'D/U
. G WGRSD 4 0 10093 0 (d a 1:500) - /Annotation : ~ ampl < 11 4 Sampl e Type : I'nknov n Concentration : N/ A Calculate d Cone : 7780 . i Acq . Data : 2/13/201 1 Ac(j . Time : 6:52:4 3 A M Moditi . Ye :
P^aK-Split
. Fa c Repor t Larges t Hin
. Pea k

Heig h Hin
. Pea k

Widt h Smootiiini j Widtl l R T Kindov : Expecte dRT : Us e Relativ e R

T int
. Type : Retentio nTime : o r : 4 •eak : N o : 0.0 0

0.0 0 3 ?0. 0 11. 7 N o va11e y 11. 7 Area : 1715668 3 c o Height : Star t Time : En dTima ; 2S30000
. c 11. 4 1C. 3



# I Sampt e Name ; 'fl110211a022 " Sampt e ID : '10007-27-11 ' Fito : 'fl1102 1 la.wi T Pea k Name : "PFOS " Uass(es} ; '499.0/99. 0 amu.499.0/60. 0 amu.499.0/130. 0 amu ' Conwnen t "1 0 ngAn L F C st d i n Mil H Q wate rAnnotation : ~ Sampl e Inde x p i e Standar d 10. 0 5.7 2 2/12/201 1 

# I Sampt e Name : "g110211a028 " Sampt e ID : "Solven t Blank - Fite : "g110211a.wf r Pea k Name : 'PFOS * Uassfes} : "499.0/99. 0 anuJ.499.D/80. 0 amu.4g9.D/130. 0 ami T Comment"MafiOV\ibterTN11-0052 ' /^notation: ' Sampl e Index : 2 9 g/m L

Repor t Larges t

Pea k Min
. Pea k

Height ; Min
. Pea k

Width : Smoothin g Width : R T Window : Expecte dRT : U3 0 Relativ e RT : Int
. Typt : \ Retentio nTime : Area : 159-1 2 : H 0 3 30. 0 12. 4 N o alle y 12. 4 90 6 Height : 3710000
. Star t Time : En dTime : 12, 3 12. 5 3.6e6

- 3.4a G 3.2e 6 3.0e 6 2.8a 6 Z6eG

Z4e6

- Z2B 6 Z0e 6 1.8e 6 1.6e 6 1.4e6 ' 1.2e 6 I.Oe S S.0e 5 6.0e 5 4.0e 5 ZOe S 0. 0 Sam p Con e Cal c Acq
. Acq
. Hod i R TW Exp e e Type : nttation : late d Co n Date : Time : ied : ndov : .-te d RT : Unknow n N/ A c ; O.OOBO S 2/12/201 1 5 ; 02;5 1 A

M Ve a 30. 0 12. 4

1 5 i e 1 7 9 1 0 n 1 2 1 3 1 4 1 5 I B 1 7 Dat aprinte db y ST W Printin g Time : 12:33:4 2 P M Printin g Date : Tuesday , Marc h22 , Pag e 3 o f 4 201 1 Pag e 6 2 o f8 3Ginge r AG0133050 9 GLPIO-01-02 ; Interi m Repor t 0 9 Res^P?l^%S|PF^'(^ ftfaS.'M ^ PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0

Calculate dCone : Acq
. Date : i Acq
. Time : 6 Modified : Proc
. Algorithm : I Nois e Percentage : Ba.-^e
. Sub
. Windcw : Peak-Split
. Facto r Repor t Latyes t

Pe a Min
. Pea k

Height : Min , Pea k

Width : Smoothin g Viidth : R TWindow : E.'<pecte dRT : 4.9 3 12/2 0 32:5 7 N o tell i 5 0 1 , 2 : H 0 1 30. 0 12. 4 

# I Sampl e Nante : 'g110211a03 rSampt e ID : "LCS-110210-5 " Pea k Name : 'PFOS ' Uass(es} ; "499.0/99. 0 amu,49g.Q/80. 0 a i Commen t "Spp b LCS " Annotatten : ~
1.ge 6 1.8e 6 1.7e 6 1.6e 6 1.Se 6 1.4e 6 1.3e 6 1.2e 6 1.1e 6 1.0e 6 9.0e 5 8.0e 5 7.0e 5 6.0e 5 5.0e 5 4.0e 5 3.0e 5 Z0e 5 I.Oe S 0. 0 

# I Sampt e Name : "g110211a042 " Sampt e ID : "LCS-110210-14 " Fite ; "g110211a.wi r Pea k Mame ; 'PFOS * Mass(e$) : "499.0/99. 0 amu,499.0/80. 0 amu,499.0/130. 0 amu " Commen t 'Spp b LC S EC P Annotation ; " Sampl e Inde:i : 4 2 Sampl e Type : 5.0 4 Calculate dC Acq
. Date : Acq
. Time : Modified : R TWindow : Expecte dRT : Us e Relativ e Int
. Type : Retentio nT i Area : Hsight : n c

R T

5
. 1 1 5.3 e ^/13/2 0 '^;14:5 9 Ve s 30. 0 12. 4 N o Manua l 1--. A •53 2 2 0 0 00
. ng/m L 1.4e 6 1.3e6 - 1.2e 6 1.1e 6 1.0e 6 g.Oe S B.OsS T.Oe S SOe S SOe S
4.0e 5 3.0e S ZOe S I.Oe S
0. 0 1 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7 

# I Sampt e Name ; "g110211a114 * Sampt e ID : •GLP1[W)1-02-O9-O2S " Pea k Nanrw : 'PFOS * Mass(es) ; "499.0/99. 0 amu,499.0/80. 0 amu,4 S Commen t "DA L G WGRS0 4 0 10093 0 (di l 1 :S00) " /Annotation : * "

culate dC

. Date :

. Time : ified : Window : ecte dRT : ReUtiv e

. Type : ght : Time : :>nc : 1310
.

2/13/2 0 E:52:4 3 Ye s 30. 0 12. 4 RT : N o Hanua l n e ; 12. 2 452713 7 < 522000
. 13. 0 12. 8 ng/m L 1 A H

mi n

C f 3

s. S.Oe S

4.8e 5 4.6e S 4.4e 5 4.2e 5 4.0e S 3.8e 5 3.66 5 3.4e S 3.2e 5 3.0e 5 2.Se 5 2.6e 5 2.4e S 2.2e 5 ZOe S 1.8e S 1.6e 5 1.4e 5 1.2e 5 I.Oe S 8.0e 4 6.0e 4

1 1 1 2 1 3 1 4 1 5 1 6 1 7 Dat aprinte db y ST W Printin g Time : 12:33:4 2 P M Printin g Date : Tuesday , Marc h22 , Pag e 4 o f 4 201 1 Pag e 6 3 o f8 3Ginge r AG0133050 9 GLPIO-01-02 ; Interi m Repor t 0 9 Res^??lm«iPF^§.j2 ft'aS.M ' ^^^ ^ ' " Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0

Dat a printe d byST W Printin g Time : 12:30:5 9 P M Printin g Date : Tuesday , Marc h22 , 201 1 Pag e 1 o f 1 Pag e 6 4 o f 8 3Ginge r AG0133050 9 GLP10-01-02 ; Interi m Repor t 0 9 Res^5'?&'^S|PFa^.(^ f^aS.M " PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0

•g110211a.rd b (PFHS) ; "Quadratic " Regressio n (" 1 /x " weighting) ; y= -8.76e+00 3 x'^ 2 + 1.24e+00 6 x+ 4.17e+00 3 ( r= 0.9995 ) 4.2e 7 J 4.0e7 - 3.8e7 - 3.6e7 - 3.4e7 - 3.2e7 - 3.0e7 - 2.8e7 - 2.6e7 - 2.4e7 -
^ 2.2e7 -

° 2.087 - (I S < 1.8e7 - 1.6e7 - 1.487 - 1.2e7 - 1.0e7 - 8.0e6 - e.OeS 4.0e6 - 2.0e6 - 0.0 - ,y- ^
^y-' ' 

### y / ' , / 

## y ' \ 

# y ' y ' y 

# y y y ' 

# y ' y ' 

# y y y / 

### / / 

# y
/ 0 2 4 6 8 1 0 1 2 1 4 1 6 1 8 2 0 2 2 2 4 2 6 2 8 3 0 3 2 3 4 Concentration , ng/m L ^

3 6 ^y-^ "

3 8 ^-•^''' ^

4 0 ^y.-" "

4 2 ^ - -

1 4 4 ^.-"" ^

4 6 4 8 • .

5 0
Dat a printe d b

yST W Printin g Time : 12:31:1 7 PM Printin g Date : Tuesday , Marc h22 , 201 1 Pag e 1 o f 1 Pag e 6 5 o f 8 3Ginge r AG0133050 9 GLPIO-01-02 ; Interi m Repor t 0 9 Res^P?&'^SAPF^?.j2!^aS.,m d pro s i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0 g1102 1 la.rd b (PFOS) ; "Quadratic " Regressio n (" 1 /x " weighting) : y= -1.24e+00 4 x'^ 2 + 1.76e+00 6 x+ 8.61e+00 3 ( r= 0.9998 ) 5.9e 7 5.5e7 -
5.0e 7
4.5e 7
4.0e 7
3.5e 7
5 3.0e 7 o u ra" 0 ) 2.5e7 -
2.0e 7
1.5e 7
1.0e7

-
5.0e6 -
0. 0 y

y


# X / 

# y

8 1 0 1 2 1 4 1 6 1 8 2 0 2 2 2 4 2 6 2 8 3 0 3 2 3 4 3 6 3 8 4 0 4 2 4 4 4 6 4 8 5 0 Concentration , ng/m L Dat a printe d b

yST W Printin g Time : 12:31:3 4 PM Printin g Date : Tuesday , Marc h22 , 201 1 Pag e 1 o f 1 Pag e 6 6 of 8 3Buste r

J293020 3 GLP10-01-02 ; Interi m Repor t 0 9 Bat^Ra!ym? f?fmi?^Hc&i?" d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0

•Sampl e Name;-bUOSlSaOIS " Sampt e ID:'10007-27-6 ' Fite:'b l 1031 Sa.wi T •Pea k Name ; "PFOS " Mass(es):'499.0/130.0amu,499.0/99.0amu,4g9.oraO.Oamu " 5arapl e Index : 1 9 Sampl e Type : Standar d Concentration : 0.25 0 ng/m L Calculate d Cone : 0.25 0 ng/m L ,3- 4 Acq
. Date : 3/15/201 1 Acq
. Time : 9:45:5 5 P H 1.3e 4 Modified : N o '"^• ^ Proc
. Algorithm : IntelliQua n - lO Ai,2e 4 Min
. P«a k

Height : 0.0 0 cp s Hin
. Pea k

Width : 0.0 0 se cl-l^ ^ Smoothin g WiOth : 3 point s , . ^ R TWindow : 30. 0 se c'" ^ Expecte d RT : 12. 5 mi n 1.0e 4 us e ReUtiv e RT : N o ^ ^ Int
. Type : Valle y9000. O Retentio n Time : 12. 5 mi n Area : 4351 4 count s 8500. 0 Height : ''''r. T'? ' 8000. 0 Star t Timo : 12, B m m En d Tim^ : ll'. C mi n •7500. 0

•6500. 0 1 6000. 0 5500. 0 5000. 0 4500. 0 4000. 0 3500. 0 3000. 0 2500. 0 2000. 0 1500. 0 1000. 0 500. 0 0. 0 •—<—'—1—•—1—•—1—•— 1

1 2 3 4 5 1 Sampt e Name : "b110315a04 rSamptelD;-LCS-110315-2 - Fite : "b110315a.wi r I Pea k Name : "PFOS " Mass(es) ; "499.0/130. 0 amu.499.0/99. 0 annu,499.0/80. 0 amu " •Commen t "O.Spp b LCS " Annotabon ; - Sampl e Index : 3 3 Sampl e Type : Q C Concentration : 0.A9 9 ng/m L 3 8- 4 Calculate d Cone : 0.-19 7 ng/m L Acq
. Data : :'/l£/201 1 Acq
. Time ; 2:47:3 4 AM 2.6e4 - HoJiriad : Ye s R TWindow : 30. 0 se c Expecte d RT : 12. 5 mi n i-le^ - Us e Relativ e ST : N o I„t
. Typ. , H.„ua l "•' • Retantio n Time : 12. 8 mi n Area : 9987 0 count s Hei.jht ! 2.a0e-t00 4 cp s 2.De4 - Star t Time : 12. 7 mi n Et. d Time : 12. 9 mi n 1.8e4 - re,, . u i ? B 1.4a4 - 

## 1 1.2e4 - 1.0o4 - 6000. 0 6000. 0
4000.0 ' 2000. 0

1 2 3 4 5 6.1 0 7.278.3K8.7 4 6 7 8 9

Tim e mi n

6 7 8 9 _ = = ^s,m = 12.9 2

1^58
. 1 

## L ^13.0 6

1 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7

1 2 7 9

12.47. ^ ^12.9 5 10.3 8 12.3^ ^

^„. , -13.7 5 ^15.0 9 16.5 7 1 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7 •Sampt e Name:-b110315a034 " Sampt e ID;'Sojven t Uanl TFite ; •bl10315a.Mf r •Pea k Name : "PFOS - Maw(ea);'499.0/130.0amu,499.0«9.0amu,499.0ffi0.0amu ' .'>ampl e Inde.t : 3 0 Sampl e Type : I'nknov n Concentration : N/ A2100 - CalcuUte d Cone : 0.00B0 5 ng/m L Acq
. Date : 3/16/201 1 2000 - Acq
. Time ; 12:22:5' ' A M 1900 - Moditiad : Ve a R TKindo-.v : 30. 0 s« c1800 - Expecte d BT : 12. 9 mi n I's s Relativ e RT : N o ^jao Int
. Type : Manua l -gTM
. Retentio n Time : 12. 9 mi n ^** " Area : 22 2 count s -g-- . Height ; ;.7Oe'0O 2 cp s Star t Time : 12. 9 mi n En d Time : 12. 9 mi n ^^ ^ 130 0 120 0
^ 110 0Intensi)1

9 0 0 8 0 0 7 0 0 6 0 0 5 0 0 4 0 0 3 0 0 2 0 0 1 0 0 1 2 3 4 5

•San:)pteName:"bl10315a04g - Satnpt e 10:'LCS-110315-6 " Fite ; "b110315a.wi r 1 Pea k Name : "PFOS " Mass(as) : "499.0/130. 0 anuJ.499.(V99. 0 «mu,499.0/80.0am> r Sampl e Index : 4 5 Sampl e Type : Q C Concentration : 0.50 2 ng/m L 1.9e4- ' Calculate d Cone : 0,45 6 ng/m L Acq
. Date : 3/16/201 1 ,0 ^ Acq
. Time : 4:54:1 9 A M "" ^ modified : Ye , I-?* * R TWindow : 30. 0 se c Expecte d RT : 12. 9 mi n 1.6e 4 Us e Relativ e RT : N o 1.5e 4 Int
. Type : Manua l Retentio n Time : 12. 9 mi n . ^
. Area : 9151 0 count s ^'^ ^ Hsiglit : l.STsrOO ^ cp 3 Star t Time : 12. 8 mi n 1-3» 4 En d Time : 13. 0 mi n 1.2e 4 1.1e 4

"
. 1.0e 4 1 9000. 0 8000. 0 7000. 0 6000. 0 5000. 0 4000. 0 3000. 0 2000. 0 1000. 0
1 2 3 4 5 . iiil.iiiiht. k ItaJi l 6 7 8 9 1 0

Time , mi n 126 0

1 1 

# l ^ ^13.0 9



### 1 l l 1 

# 1 1 i
. . itlbiiiL i 

# liUl l J J 1 1 1 2 1 3 1 4 1 5 1 6 l'7 |

1 2 9 1

"•" 1 t

k J^'^.J^..^ ^ 6 7 8 9 I' O i' l 1 2 1 3 1 4 1 5 1 6 iV ]

— 1 Dat aprinte db y ST

W Printin g Time : 12:35:5 9 P M Printin g Date : Tuesday , Marc h22 , 201 1 Pag e 1 o f 2 Pag e 6 7 o f8 3'* * Buste r

J293020 3 GLP10-01-02 ; Interi m Repor t 0 9 Bat^P^lym? ^

?fmi'5lt^c|i?" d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0

I Sare^pl e Name;'bl10315a035 " Sampt e ID : "GLP10-01-02-09-053 " Fite : "bUOSlSa.vvif r I Pea k Name : "PFOS ' Man(es) : "499.0/130. 0 amu,499.0/99. 0 amu.49g.0/80. 0 amu " I Comment"DALGW328L0100928 ' /Wwtation; - N/ A Acq . Date : Acq . Time : Modified : Proc . Algorithm : Min . Pea k Heigh t Hin . Pea k Width : Smoothin g width : R T Window : Expecte d RT : Us e Relativ e RT : Int . Type : Retentio n Time : Area ; 1 Height : Star t Time ; En d Tima : ;'/16/201 1 12:41:0 2 N o IntelliQ u : 0.0 0 0.0 0 3 30. 0 12. 9 N o Valle y 12. B 4550 6 C O 1.94e-f00 4 12. 7 13. 0 2.Cte4 1.9e 4 1.8e4 ' 1.7e 4 1.6e 4 1.5 M 1.4e 4 1.3e 4 1.2e 4 1.1e 4 1.0e 4 9000. 0 8000.0 - 7000.0 - 6000.0 - 5000. 0 4000. 0 3000. 0 2000. 0 1000.0 - 0. 0 

# L

/ ^mn/t,. , n 9 1 0 1 1 1 2 1 3 1 4 1 5 1 8 1 7

Dat aprinte db y ST

W Printin g Time : 12:36:5 9 P M Printin g Date : Tuesday , Marc h22 , 201 1 Pag e 2 o f 2 Pag e 6 8 o f8 3Buste r

J293020 3 6LP10-01-02 ; Interi m Repor t 0 9 Bat^R^lyiJ^^p fgf^,,l=|HSj^an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0
b l lOSISa.rd b (PFOS) : "Quadratic " Regressio n (" 1 / x " weighting) ; y= -88 5 x* 2 + 2.04e•^00 5 x+ -1.42e-H00 3 ( r= 0.9996 )
4.6e6 - 4.4e6 - 4.2e6 - 4.0e6 - 3.8e6 - 3.6e6 - 3.4e6 - 3.2e6 - 3.0e6 - 2.8e6 - 2.6e6 - 2.4e6 - 2.2e6 - 2.0e6 - 1.8e6 - 1.6e 6 1.4e6 - 1.2e6 - 1.0e6 - S.OeS e.OeS 4.0e5 - 2.0e5 - 0.0 - ^ * 

# X" " ,,•-- "

y'" ' y ' 

# y y y ' ' y ^ 

## y ' ' yii '



### y y y " "



## y " y ' y ' ' y ' ' y'-' ' y'' ' y ' y y'' ' . y

,/•-"" " 

### y

y ' 0 -

0 1 1 0 1 1 1 2 1 3 1 4 Concentration , ng/m L 

### 1 5 1 6 1 7 1 8 1 9 2 0 2 1 2 2 2 3 2 4 2 5
Dat a printe d b

yST W Printin g Time : 12:35:3 3 PM Printin g Date : Tuesday , Marc h22 , 201 1 Pag e 1 o f 1 Pag e 6 9 of 8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S I n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0


### ATTACHMEN TC : ANALYTICA L METHO D

Pag e 7 0 o f8 3

### Documen t ma ybeG!JF;alo•9:l^tf2Citlf3fl8fF!ep%t^osi 4 day s fro m 03/22/20 1 Analysi s o fPFBS , PFHS , an d PFO S I n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0


# 3 M Environmenta l Laboratoi y

Mettio d Mettio d o fAnalysi s fo rth e Determinatio n o fPerfluorinate d Compound s i n Wate r b yLC/MS/MS ; Direc t Injectio n Analysi s Metho d Number : ETS-8-044. 0 Adoptio n Date : Upo n Signin g Effectiv e Date : Q U I n f o ' 1
Approve d By : 

# ^y^/ a 

# • > Willia m K
. Reagen , Laborator yManage rDat e

ETS-8-044. 0 Pag e 1 o f1 1 Mettio d ofAnalysi s fo rth e Determinatio n o fPerfluorinate d Compound s i n Wate rb yLC/MS/MS ; Direc

t Injectio n Analysi s Pag e 71 o f8 3

### Documen t ma ybB^immj-ihaomm-ihj&sjsoi Aday s fro m 03/22/201 1 Analysi s o fPFBS , PFHS , an d PFO S I n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0


## 1 Scop e an d Applicatio n Thi s metho d i s t o b e use d t o quantif yPerfluorobutanoi cAci d (PFBA) , Perfluoropentanoi cAci d (PFPeA) , Perfluorohexanoi cAci d (PFHA) , Perfluoroheptanoi cAci d (PFHpA) , Perfluorooctanoi cAci d (PFOA) , Perfluorononanoi cAci d (PFNA) , Perfluorodecanoi cAci d (PFDA) , Perfiuoroundecanoi cAci d (PFUnA) , Perfluorododecanoi cAci d (PFDoA) , Perfluorobutanesulfonat e (PFBS) , Perfluorohexanesulfonat e (PFHS) , an d Perfluorooctanesulfonat e (PFOS ) b yHig h Performance Liqui d Chromatograph ycouple d t o a tande m Mass Spectrometri cDetecto r(LC/MS/MS ) i n clea n wate rsamples
. Wate rsample s containin g heav y particulat e ma

yrequire preparatio n b

ya n altemat e metho d suc h as ETS-8-15 4 "Determinatio n of Perfluorinate d Acids , Alcohols , Amides , an d Sulfonate s I n Wate rB ySoli d Phas e Extractio n an d Hig h Performance Liqui d Chromatography/Mas s Spectrometry"
. Thi s metho d i s considere d a performance-base d method
. Data i s considere d acceptabl e as lon g as th e define d Q C elements are satisfied
. Sampl e collectio n i s not covere d unde rthi s analytica l procedure
. 2 Metho d Summar y Clea n aqueou s sample s are analyze d b ydirec t injectio n usin g LC/MS/MS
. Sample s containin g heav y particulat e ma

ynot b e suitabl e fo ranalysi s b ythi s method
. Sample s containin g suspende d particulate shoul d b e centrifug e prio rt o removin g a sampl e aliquot , o rfiltered
. Thi s i s a perfonnance-base d method
. Metho d accurac yi s detennine d fo reac h sampl e set usin g multipl e laborator ycontro l spike s at multipl e concentrations
. Thi s metho d als o require s that th e precisio n an d accurac yfo reac h sampl e b e detennine d usin g fiel d matri xspike s t o verif ythat th e metho d i s applicabl e t o eac h sampl e matrix
. Sampl e results fo rspike s outsid e of70 % t o 130% , ma yb e flagge d as suc h (wit h expande d accurac

ystatements) , o rwil l no

t b e reporte d du e t o non-complian t qualit ycontro l samples
. Fortificatio n level s fo rfiel d matri xspike s an d fo rlaborator ymatri xspike s shoul d b e a t least 50 % ofth e endogenou s leve l an d les s tha n 1 0 time s th e endogenou s leve l t o b e use d t o determin e th e statemen t of accurac yfo ranalytica l results
. 

## 3 Definition s 3. 1 Calibratio n Standar d Asolutio n prepare d b

yspikin g a know n volum e o fth e Wori<in g Standar d (WS ) into a predetermine d amoun t ofAST M Typ e I , HPLC grad e water , o rothe rsuitabl e water , an d analyze d accordin g t o thi s method
. Calibratio n standard s are use d t o calibrat e th e instrumen t respons e wit h respec t t o analyte concentration
. 3. 2 Laborator yDuplicat e Sampl e (LDS , o rLa b Dup ) Alaborator

yduplicat e sampl e i s a separat e aliquot o fa sampl e take n i n th e analytica l laborator ythat i s analyze d separatel ywit h identica l procedures
. Analysi s ofLDS s compare d t o that ofth e first aliquot give a measur e ofth e precisio n associate d wit h laborator yprocedures , bu t no

t wit h sampl e collection , preservation , o rstorag e procedures
. 3. 3 Fiel d Blan k

(FB)/Tri p Blan k AST M Typ e I , HPLC grad e water , o rothe rsuitabl e water , place d i n a sampl e containe ri n th e laborator y an d treate d as a sampl e i n al l respects , includin g exposure t o samplin g sit e conditions , storage , preservatio n an d al l analytica l procedures
. Th e purpos e o fth e F B i s t o determin e i ftest substance s o r othe rinterference s are presen t i n th e fiel d environment
. Thi s sampl e i s als o refen-ed t o as a Tri p Blank
. ETS-8-044. 0 Pag e 2 o f1 1 Metho d ofAnalysi s fo rth e Determinatio n o fPerfluorinate d Compound s i n Wate rbyLC/MS/MS ; Direc t Injectio n Analysi s Pag e 7 2 o f8 3

### Documen t ma yh&immii^^hcmstifrrikjhxoi Aday s fro m 03/22/201 1 Analysi s o fPFBS , PFHS , an d PFO S I n Groundwate r Quarteri yGroundwate rSamplin g - Sep t 201 0


## 3. 4 Fiel d Duplicat e Sampl e (FDS , Fiel d Dup ) Asampl e collecte d i n duplicat e a t th e sam e tim e fro m th e sam e locatio n as th e sample
. Th e FD S i s handle d unde ridentica l circumstance s an d treate d exactl yth e sam e throughou t fiel d an d laborator y procedures
. Analysi s ofth e FD S compare d t o tha t ofth e first sampl e give s a measure ofth e precisio n associate d wit h sampl e collection , preservatio n an d storage , as wel l as wit h laborator yprocedures
. 3. 5 Fiel d Matri xSpik e (FMS ) Asampl e t o whic h know n quantitie s ofth e target analyte s are adde d t o th e sampl e bottl e i n th e laborator y befor e th e bottle s are sen t t o th e fiel d fo rcollectio n o faqueou s samples
. Aknown , specifi cvolum e of sampl e must b e adde d t o th e sampl e containe rwithou t rinsing. Thi s ma yb e accomplishe d b

ymakin g a "fil l t o thi s level " lin e o n th e outsid e o fth e sampl e container
. Th e FM S shoul d b e spike d betwee n approximatel y50 % an d 1 0 time s th e expecte d analyt e concentratio n i n th e sample
. I fth e expecte d rang e ofanalyt e concentration s i s unknown , multipl e spike s a t varyin g level s ma

yb e prepare d t o increas e th e likelihoo d that a spike a

t a n appropriat e leve l i s made
. Th e FM S i s analyze d t o ascertai n i fan ymatri x effects , interferences , o rstabilit yissue s ma

ycomplicat e th e interpretatio n ofth e sampl e analysis
. 3. 6 Tri p Blan k

Spik e (Fiel d Spik e Contro l Sample , FSCS ) A n aliquot ofAST M Typ e I , HPLC grad e water , o rothe rsuitabl e water , t o whic h know n quantitie s ofth e target analyte s are adde d i n th e laborator yprio rt o th e shipmen t ofth e collectio n bottles
. Th e FSC S i s extracte d an d analyze d exactl ylik e a stud ysampl e t o hel p determin e i fth e metho d i s i n contro l an d whethe ra los s o fanalyt e coul d b e attribute d t o holdin g time , sampl e storag e and/o rshipmen t issues
. A lo wan d hig h FSC S are appropriat e whe n expecte d sampl e concentration s are no t know n o rma

yvary . At least on e separate , un-spike d sampl e mus t b e take n a

t th e sam e tim e an d place as eac h FMS
. 3. 7 Laborator yContro l Sampl e (LCS ) A n aliquot ofcontro l matri xt o whic h know n quantitie s o fth e targe t analyte s are adde d i n th e laborator ya t th e tim e ofsampl e extraction
. At least two level s are included , on e generall yat th e lo wen d ofth e calibratio n curve an d on e nea rth e mi d t o uppe rrang e ofth e curve . Th e LCS s are extracte d an d analyze d exactl ylik e a laborator

ysampl e t o detennin e whethe rth e metho d i s i n control
. LCS s shoul d b e prepare d eac h da ysample s are extracted
. 3. 8 Laborator yMatri xSpik e (LMS ) Alaborator

ymatri xspike i s a n aliquot ofa sampl e t o whic h know n quantitie s oftarge t analyte s are adde d i n th e laboratory
. Th e LM S i s analyze d exactl ylik e a laborator

ysampl e t o detemriin e whethe rth e sampl e matri xcontribute s bias t o th e analytica l results
. Th e endogenou s concentration s ofth e analyte s i n th e sampl e matri xmust b e determine d i n a separat e aliquot an d th e measure d value s i n th e LM S correcte d fo rthes e concentrations
. LMS s are optiona l fo ranalysi s ofaqueou s samples
. 3. 9 Laborator ySampl e Aportio n o raliquot ofa sampl e receive d fro m th e fiel d fo rtesting
. 

## 3.1 0 Limi t o fQuantitatio n (LOQ ) Th e lowe rlimi t ofquantitatio n (LLOQ ) fo ra dataset i s th e lowest concentratio n that ca n b e reliabl y quantitate d withi n th e specifie d limits o fprecisio n an d accurac ydurin g routin e operatin g conditions
. To simplif ydat a reporting , th e LLO Q i s generall yselecte d as th e lowest non-zer o standar d i n th e calibratio n curve that meets metho d criteria
. Sampl e LLOQs are matrix-dependent
. Th e uppe rlimi t ofquantitatio n (ULOQ ) fo ra dataset i s th e highest concentratio n that ca n b e reliabl y quantitate d withi n th e specifie d limits ofprecisio n an d accurac ydurin g routin e operatin g conditions
. Th e highest standar

d i n th e calibratio n curve that meets metho d criteri a i s define d as th e ULOQ
. ETS-8-044. 0 Pag e 3 o f1 1 Metho d o fAnalysi s fo rth e Determinatio n o fPerfluorinate d Compound s i n Wate rb yLC/MS/MS ; Direc

t Injectio n Analysi s Pag e 7 3 o f8 3

### Documen t ma ybeGLFai(M(l^b'2al^t3Tii:.fF.!epfetiOE'!4 day s fro m 03/22/201 1 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0



### 3.1 1 Metho d Blan k A n aliquot o fcontro l matri xthat i s treate d exactl ylik e a laborator

ysampl e includin g exposure t o al l glassware , equipment , solvents , an d reagents that ar e use d wit h othe rlaborator ysamples
. Th e method blan k i s use d t o determin e i ftest substance s o rothe rinterference s are presen t i n th e laborator y environment , th e reagents , o rth e apparatus
. 3.1 2 Sampl e Asampl e i s a n aliquot remove d fro m a large rquantit yofmateria l intende d t o represen t th e origina l source material
. 3.1 3 Stoc k

Standar d Solutio n (SSS ) Aconcentrate d solutio n ofa single-analyt e prepare d i n th e laborator ywit h a n assaye d reference compound
. 3.1 4 Surrogat e Acompoun d simila ri n chemica l compositio n an d behavio rt o th e target analyte(s) , bu t i s no t normall y foun d i n th e sample(s)
. Asun-ogat e compoun d i s typicall ya target analyt e wit h a t least on e ato m containin g a n isotopically-labele d substitution
. I fused , surrogate(s ) ar e adde d t o al l sample s an d qualit y contro l samples
. Sun'ogate(s ) are adde d t o quantitativel yevaluat e th e entire analytica l procedur e includin g sampl e collection , preparation , an d analysis
. Inclusio n ofa sun'ogate analyt e i s a n optiona l qualit ycontro l measure an d i s NOTrequired
. 

## 3.1 5 Workin g Standar d (WS ) Asolutio n ofsevera l analyte s prepare d i n th e laborator yfro m SSS s an d dilute d a s neede d t o prepar e calibratio n standard s an d othe rrequire d analyte solutions
. 4 Warning s an d Caution s 4. 1 Healt h an d Safet y Th e acut e an d chroni ctoxicit yofth e standard s fo rthi s metho d have not bee n precisel ydetermined ; however , eac h shoul d b e treate d as a potentia l healt h hazard
. Th e analyst shoul d wea rgloves , a la b coat , an d safet

yglasse s t o preven t exposure t o chemical s that migh t b e present
. Th e laborator

yi s responsibl e fo rmaintainin g a saf e wori < environmen t an d a cun-ent awarenes s o floca l regulation s regardin g th e handlin g ofth e chemical s use d i n thi s method
. Areference fil e ofmateria l safet y dat a sheets (MSDS ) shoul d b e availabl e t o al l personne l involve d i n thes e analyses
. 

## 4. 2 Caution s Th e analyst must b e familia rwit h th e laborator yequipmen t an d potentia l hazard s including , bu t no t limite d to , th e us e o fsolvents , pressurize d gas an d solven t lines , hig h voltage , an d vacuu m systems
. Refe rt o th e appropriat e equipmen t procedure o roperato rmanua l fo radditiona l informatio n an d cautions
. 

## 5 Interference s Durin g sampl e preparatio n an d analysis , majo rpotentia l contaminan t source s are reagents an d glassware
. Al l material s use d i n th e analyse s shal l b e demonstrate d t o b e fre e fro m interference s unde r condition s ofanalysi s b

ymnnin g metho d blanks
.

ETS-8-044. 0 Pag e 4 o f1 1 Metho d o fAnalysi s fo rth e Determinatio n o fPerfluorinate d Compound s i n Wate rb yLC/MS/MS ; Direc

t Injectio n Analysi s Pag e 7 4 o f8 3

### Documen t ma ybe3^JlS1©#^ci2ClataHBf1feFiM•o£i 4 day s fro m 03/22/20 1 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarteri yGroundwate rSamplin g - Sept 201 0


### Parts an d supplie s that contai n Teflon® shoul d b e avoide d o rminimize d du e t o th e possibilit yof interference and/o rcontamination
. Thes e ma yinclude , bu t are no

t limite d to : was h bottles , Teflon ® line d caps , autovia l caps , HPLC parts , etc
. Th e us e o

fdisposabl e micropipette s o rpipette s t o aliquot standar d solution s i s recommende d t o mak e calibratio n standard s an d matri xspikes
. 

## 6 Instrumentation , Supplies , an d Equipmen t 6. 1 Instrumentatio n an d Equipmen t Ahig h perfonnance liqui d chromatograp h capabl e ofpumpin g u p t o two solvents an d equippe d wit h a variabl e volum e injecto rcapabl e ofinjectin g 5-10 0 \3 L connecte d t o a tande m Mas s Spectromete r (LC/MS/MS)
. I Analytica l balance capabl e o freadin g t o 0.000 1 g Adevice t o collec

t ra wdat a fo rpea k

integratio n an d quantitatio n 15-m L an d 50-m L disposabl e polypropylen e centrifug e tubes
. Gas tigh t syringes , 25|JL , 50|JL , 100|JL , 250|JL , 500|JL , 1000|JL
. 

### 1 m L plasti cHPLC autovial
. Disposabl e pipettes , polypropylen e o rglas s as appropriat e Centrifug e capabl e o fspinnin g 15-m L an d 50-m L polypropylen e tube s a t 300 0 rpm . 6. 2 Chromatographi cSyste m Guar d Column : Pris m RP , 4. 6 m m x5 0 mm , 5 p m Analytica l Column : Betasi l C18,4. 6 m m x10 0 mm , 5 p m Temperature : 10° C Mobil e Phas e (A) : 2 m M Ammoniu m Acetat e i n Wate r Mobil e Phas e (B) : Methano l Gradien t Program :

Tim e (min ) 0. 0 0. 5 11. 0 13. 5 13. 6 17. 0 % A

9 7 9 7 5 5 9 7 9 7 % B

3 3 9 5 9 5 3 3 FlowRat e

(mL/min ) 1. 0 1. 0 1. 0 1. 0 1. 0 1. 0 Injectio n Volume : 10 0 pL
. Quantitation : Pea k

Are a - quadrati ccurve fit , 1/ xweighted
. RunTime : ~ 17minutes
. f 

### Th e previou s informatio n i s intende d a s a guide ; alternat e condition s an d equipmen t mayb e use d provide d tha t dat a qualityobjective s are met
.
ETS-8-044. 0 Pag e 5 o f1 1 Metho d o fAnalysi s fo rth e Determinatio n o fPerfluorinate d Compound s i n Wate rb yLC/MS/MS ; Direc

t Injectio n Analysi s Pag e 7 5 o f8 3

### Documen t ma ybesijiBs^n^hcMmrnie^soi Aday s fro m 03/22/201 1 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0


## 6. 3 MS/M S Syste m 6.3. 1 Mode : Electrospra yNegativ e ion , MR M mode , monitorin g th e followin g transitions : Analyt e PFBA PFPeA PFH A PFHpA PFOA PFN A PFD A PFUn A PFDoA PFB S PFH S PFO S Transitio n Monitore d 213^16 9 263- > 21 9 313-^26 9 an d 31 3 ^11 9 36 3 - ^ 319 , 36 3 ^

16 9 an d 36 3 - > 11 9 41 3 ^ 369 , 41 3 ^

21 9 an d 41 3 - ^

16 9 46 3 - > 419 , 46 3 - > 16 9 an d 46 3 - ^ 21 9 51 3 - > 469 , 51 3 - ^

21 9 an d 51 3 ^

26 9 56 3 ^ 519 , 56 3 - > 26 9 an d 56 3 ^21 9 61 3 - ^ 569 , 61 3 - ^

16 9 an d 61 3 - > 31 9 29 9 ^ 8 0 an d 29 9 - ^

9 9 39 9 - > 8 0 an d 39 9 - > 9 9 49 9 - > 80 , 49 9 - ^

9 9 an d 49 9 ^

13 0 Multipl e transition s fo rmonitorin g th e analyte s i s a n option , as summin g multipl e transition s ma yprovid e quantitatio n o fisomers tha t more closel ymatche s NM R dat a an d mayhav e th e adde d benefi t o f increase d sensitivity
. Th e us e o fon e daughte rio n i s acceptabl e i fmetho d sensitivityi s achieved , provide d tha t retentio n tim e criteri a ar e me t t o assur e adequat e specificity
. Th e previou s infonnatio n i s intende d as a guide , altemat e instruments an d equipmen t ma yb e used
. 7 Reagent s an d Standard s 7. 1 Chemical s Wate r- Milli-Q , HPLC grade , o rothe rsuitabl yappropriat e source s Methano l - HPLC grad e Ammoniu m Acetat e -A.C.S
. Reagen t Grad e 7. 2 Standard s Perfluorobutanoi cAci d (PFBA-C 4 acid) ; Oakwoo d Products , In c Perfluoropentanoi cAci d (PFPeA- C5 acid , als o know n as NFPA , nonafluoropentanoi cacid) ; Alf a Aesa r Perfluorohexanoi cAci d (PFHA-C 6 acid) ; Oakwoo d Products , In c Perfluoroheptanoi cAci d (PFHpA- C 7 acid , als o know n as TDHA , tridecafluoroheptanoi cacid) ; Oakwood Products , In c Perfluorooctanoi cAci d (PFOA- C 8 acid) ; 3 M Perfluorononanoi cAci d (PFN A- C 9 acid) ; Oakwoo d Products , In c Perfluorodecanoi cAci d (PFDA-CI O acid) ; Oakwoo d Products , In c Perfiuoroundecanoi cAci d (PFUnA-C1 1 acid) ; Oakwoo d Products , In c Perfluorododecanoi cAci d (PFDoA- C l 2 acid) ; Oakwoo d Products , In c Perfluorobutanesulfonat e (PFB S - C 4 sulfonate) ; 3 M Perfluorohexanesulfonat e (PFH S - C 6 sulfonate) ; 3 M Perfluorooctanesulfonat e (PFO S - C 8 sulfonate) ; 3 M ETS-8-044. 0 Pag e 6 o f1 1 Metho d ofAnalysi s fo rth e Determinatio n o fPerfluorinate d Compound s i n Wate rbyLC/MS/MS ; Direc t Injectio n Analysi s Pag e 7 6 o f8 3

### Documen t ma ybeGLBie^j-ifepatsrafFiepiMo M day s fro m 03/22/20 1 • Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0


### Th e previou s informatio n i s intende d as a guide
. Reagent s an d standard s fro m alternat e source s ma yb e used
. 7. 3 Reagen t Preparatio n 2 m M Ammoniu m acetat e solutio n (Analysis)—^Weig h 0. 3 g ofAmmoniu m acetat e an d dissolve i n 2. 0 L o freagen t water
. Note : Altemativ e volume s ma yb e prepare d as lon g as th e ratio s ofth e solven t t o solut e ratio s ar e maintained
. 7. 4 Stoc k

Standar d Solutio n (SSS ) an d Workin g Standar d Solutio n Preparatio n Th e followin g standar

d preparatio n procedure serve s as a n example
. Weighe d amounts an d fina l volume s ma yb e change d t o sui t th e neeids o fa particula rstudy
. Fo rexample , p L volume s ma yb e spike d into volumetri cflask s whe n dilutin g stoc k

solution s t o appropriat e levels
. 100 (jg/m L targe t analyt e SSSs—^Weig h ou t 1 0 m g ofanalytica l standar d (correcte d fo rpercen t sal t an d purity ) an d dilut e t o 10Om L wit h methano l o rothe rsuitabl e solvent , i n a 10Om L volumetri cflask
. Transfe rt o a 125m L LDP E bottl e o rothe rsuitabl e container
. Prepar e a separat e solutio n fo reac h analyte
. Expiratio n date s an d storag e condition s ofstoc k solution s shoul d b e assigne d i n accordance wit h laborator ystandar d operatin g procedure
. A n exampl e o fpurit yan d sal t correctio n i s give n belowfo rPFOS
. ^
. , ^ molecula rweigh t o fanio n sal t correctio n facto r= = moclecula rweigh t o fsal t

49 9 PFO S (K^

)sal t correctio n facto r= ^^ ^ = 0.927 5 53 8 10m g CsFiySOsK*wit h purit y90 % = 8.35m g C8F17SO3"(1 0 mg*0.90*0.9275=8.3 5 mg ) 5 Mg/m L (500 0 ng/mL ) mixe d workin g standard—Ad d 0.5m L eac h ofth e 10Opg/m L SSS s t o a 10m L volumetri c flas k an d brin g u p t o volum e wit h solvent
. 25 0 ng/m L mixe d workin g standard—Ad d 1.25m L ofth e 5 pg/m L -mixe d worthin g standar d solutio n t o a 25m L volumetri cflas k

an d brin g u p t o volum e wit h solvent
. 12 5 ng/m L mixe d standard—^Ad d 625p L o fth e 5 pg/mL-mixe d worthin g standar d solutio n t o a 25m L volumetri c flas k an d brin g u p t o volum e wit h solvent
. Storag e Conditions—Stor e al l SSS s an d wori<in g standard s i n accordance wit h laborator ystandar d operatin g procedur e o ri n a refrigerato rat 4°±2° C fo ra maximu m perio d of6 month s fro m th e dat e ofpreparation
.

ETS-8-044. 0 Pag e 7 o f1 1 Metho d o fAnalysi s fo rth e Determinatio n o fPerfluorinate d Compound s i n Wate rbyLC/MS/MS ; Direc t Injectio n Analysi s Pag e 7 7 o f8 3

### Documen t ma ybeG!JSt^><l!l^Lf2ClWS?ffi^n%p%ti-o£'! 4 day s fro m 03/22/201 1 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0



### 7. 5 Calibratio n Standard s Usin g th e wori<in g standard s describe d above , prepar e calibratio n solution s i n AST M Typ e I water , HPLC water , o r othe rsuitabl e water , usin g th e followin g tabl e as a guideline
. Note : Volume s ofwate ran d workin g standard s ma y b e adjuste d t o meet th e dat a qualit yobjective s addresse d i n th e genera l projec t outline
. Calibratio n level s othe r tha n thos e liste d belowca n b e prepare d as needed
.
Concentratio n o fWS , ng/m L 12 5 12 5 12 5 12 5 25 0 25 0 25 0 25 0 500 0 500 0 500 0 Volum e o fWS.fd
.

1 0 1 5 2 0 3 0 2 0 5 0 10 0 20 0 2 5 5 0 10 0 Fina l Volum e o f

Calibratio n Standar d (m L o fAST M Typ e 1 Water , o r othe rsuitabl e water )

5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 5 0 Fina l Concentratio n o f

Calibratio n Standard , ng/m L (ppb ) i n ASTIV I Typ e 1 Water , o rothe rsuitabl e wate r 0.02 5 0.037 5 0.05 0 0.07 5 0.10 0 0.25 0 0.50 0 1.00 2.5 0 5.0 0 10. 0 

### 8 Sampl e Handlin g 8. 1 Wate rSampl e Preparatio n Thi s metho d i s applicabl e t o clea n wate rsamples
. Sample s containin g heav yparticulat e ma

yno t b e suitabl e fo r analysi s b

ythi s method
. Sample s containin g suspende d particulat e shoul d b e centrifug e prio rto removin g a sampl e aliquot , o rfiltered
. Thoroughl ymi xsampl e befor e removin g a n aliquot an d placin g i n a labele d plasti cautovial
. Plasti ci s prefen-ed ove rth e us e ofglas s autovials , t o preven t th e possibl yoffluorochemica l stickin g t o th e glass
. Dilut e sample , i fnecessary , wit h AST M Typ e I , HPLC water , o rothe rsuitabl e water
. Prepar e metho d Q C sample s an d multipl e metho d blank s an d aliquot into labele d plasti cautovials
. Prepar e at least fiv e metho d blanks
. 

## 9 Sampl e Analysi s - LC/MS/M S Analyz e th e standard curve prio rt o eac h set o fsamples
. Th e standar d curve ma

yb e plotte d usin g a linea rfit , weighte d 1/

xo runweighted , o rbyquadrati cfi l ( y= ax ^ + bx+ c) , weighte d 1/

xo runweighted , usin g suitabl e software
. Th e calibratio n curve s ma yinclud e bu t shoul d no

t b e force d throug h zero . Th e mathematica l metho d use d t o calculat e th e calibratio n curve shoul d b e applie d consistentl ythroughou t a study
. An ychang e shoul d b e thoroughl ydocumente d i n th e ra wdata
. Hig h and/o rlo

wpoints ma

yb e exclude d fro m th e calibratio n curve s t o provid e a bette rfi t ove rth e rang e appropriat e t o th e dat a o rbecaus e theydi d no t meet th e pre-determine d acceptance criteria
. Low-leve l curve points shoul d als o b e exclude d i fthei rare a counts ar e no t a t least twic e that o fth e metho d and/o rsolven t blanks
. Th e coefficien t ofdetenninatio n (r^ ) valu e fo rth e calibratio n curve must b e greate rtha n o requa l t o 0.990
. Eac h poin t i n th e curve must b e withi n ±25 % ofth e theoretica l concentratio n wit h th e exceptio n ofth e LLOQ , whic h ma y ETS-8-044. 0 Pag e 8 o f1 1 Metho d o fAnalysi s fo rth e Determinatio n o fPerfluorinate d Compound s i n Wate rb yLC/MS/MS ; Direc

t Injectio n Analysi s Pag e 7 8 o f8 3

### Documen t ma ybeG!.iFal)#^i/2Clat'3m^n1ep%,tro£'l 4 day s fro m 03/22/201 1 Analysi s o fPFBS , PFHS , an d PFO S I n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0


### b e withi n ±30%
. Justificatio n fo rexclusio n ofcalibratio n curve points wil l b e note d i n th e ra wdata
. Aminimu m of6 points wil l b e use d t o construc t th e calibratio n curve . I fth e calibratio n curve doe s no t meet acceptance criteria , perfom i routin e maintenance o rprepar e a ne wstandar d curve (i fnecessary) an d reanalyze
. Continuin g calibratio n verification s (CCV) are analyze d t o verif yth e accurac

yofth e calibratio n curve . Analyz e a mid-rang e calibratio n standard , on e ofth e sam e standard s use d t o constmc t th e calibratio n curve , a

t a minimu m afte rever ytent h sample , not includin g solven t blanks , wit h a minimu m ofon e pe rsampl e set
. Calibratio n verificatio n injection s must b e withi n ±25 % t o b e considere d acceptable
. Th e calibratio n curve an d th e las t passin g CCV wil l the n bracket acceptabl e samples
. Multipl e CCV level s ma yb e used
. Sample s containin g analyte s that ar e quantitate d above th e concentratio n ofth e highest standar d i n th e curve shoul d b e furthe rdilute d an d reanalyzed
. 1 0 Qualit yContro l 10. 1 Dat a Qualit yObjective s Thi s metho d an d require d qualit ycontro l sample s i s designe d t o generat e dat a accurat e t o ±30 % wit h a targete d LOQ o f0.02 5 ng/mL
. An ydeviation s fro m th e qualit ycontro l measure s spelle d ou t belowwil l b e documente d i n th e ra wdat a an d footnote d i n th e fina l report
. 10. 2 Metho d Blank s Metho d blank

s must b e prepare d wit h eac h analysi s batch
. At least fiv e metho d blank s must b e prepared
. Metho d blank

s ma yb e injecte d multipl e times , bu t n o mor e tha n 3 injection s shoul d b e remove d fro m a singl e metho d blank
. At a minimum , metho d blank s ar e analyze d prio rt o instrumen t calibration , prio rto th e analysi s of CCV samples , an d at th e en d ofth e analytica l mn . Th e mea n are a coun t fo reac h analyt e i n th e metho d blank s must b e les s tha n 50 % ofth e are a coun t ofth e LOQ standard
. Th e standar

d deviatio n o fth e are a counts ofthes e metho d blank s shoul d b e calculate d an d reported
. I f th e mea n are a counts o fth e metho d blank s excee d 50 % ofth e LOQ standard , the n th e LOQ mus t b e raise d t o th e first standar

d leve l i n th e curve that meets criteria , o raltematively , th e metho d blank s must b e evaluate d statisticall yt o determin e outliers , o rtechnica l justificatio n t o eliminat e on e o rmor e results shoul d b e made
. 

## 10. 3 Sampl e Replicate s Sample s duplicate s are collecte d i n th e field
. Th e relative percen t difference , RPD , shoul d b e reported
. RP D results greate rtha n 20 % wil l b e flagge d i n th e report , bu t wil l no t b e exclude d fro m reporting
. Th e requiremen t fo r replicate s exclude s fiel d blanks
. 10. 4 Surrogat e Spike s Surrogate spike s are no

t require d bu t ma

yb e use d o n projec

t specifi crequirements
. 10. 5 La b Contro l Sampl e Triplicat e la b contro l spike s a t a minimu m o

ftwo differen t concentration s are t o b e prepare d wit h eac h preparatio n batch
. Lo wla b contro l spike s shoul d b e prepare d a t concentration s i n th e rang e offiv e t o te n time s highe rtha n th e targete d LOQ an d hig h la b contro l spike s shoul d b e prepare d a t concentration s nea rth e mid-poin t ofth e curve . Th e relative standar

d deviatio n ofth e contro l spike s evaluate d independentl ya t eac h concentratio n leve l mus t b e les s tha n o requa l t o 20 % an d th e averag e recover ymus t b e 80-120%
. I fth e above criteri a are no t met , th e entire set ofsample s shoul d b e re-injecte d o rre-prepare d as appropriate
.

ETS-8-044. 0 Pag e 9 o f1 1 Metho d o fAnalysi s fo rth e Detenninatio n o fPerfluorinate d Compound s i n Wate rbyLC/MS/MS ; Direc t Injectio n Analysi s Pag e 7 9 o f8 3

### Documen t ma ybe^iM^^i^amtifrnhpcsso^i Aday s fro m 03/22/201 1 Analysi s o fPFBS , PFHS , an d PFO S I n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0



### 10. 6 Fiel d Matri xSpike s / La b Matri xSpike s Recoverie s offiel d matri xspike s an d laborator ymatri xspike s are anticipate d t o b e betwee n 70 % an d 130 % o fth e fortifie d levels
. Sampl e results fo rspike s outsid e o f70 % t o 130% , ma

yb e flagge d as suc h (wit h expande d accurac ystatements) , o rwil l no

t b e reporte d du e t o non-complian t qualit ycontro l samples
. Th e targete d fortificatio n level s shoul d b e a t least 50 % ofth e endogenou s leve l an d les s tha n 1 0 time s th e endogenou s leve l t o b e use d withou t justificatio n t o detennin e th e statemen t o faccurac yfo ranalytica l results
. Th e averag e ofth e sampl e an d th e fiel d duplicat e shoul d b e use d t o calculat e th e recovery . 1 1 Dat a Analysi s an d Calculation s Use th e followin g equatio n t o calculat e th e amoun t ofanalyt e foun d (i n ng/mL , base d o n pea k area) usin g th e standar d curve (linea rregressio n parameters ) generate d b ya n appropriat e software program : A, 1 r.J , , l ^

(Pea k Area-Intercept ) ^ ^ Analyt e foun d (ng/mL ) = - ^ ^-^

xD F Slop e D F = facto rb

ywhic h th e fina l volum e wa s diluted , i fnecessary . Fo rsample s fortifie d wit h know n amounts ofanalyt e prio rt o extraction , us e th e followin g equatio n t o calculat e th e percen t recovery .

_Tota l analyt e foun d (ng/mL ) - Averag e analyt e foun d i n sampl e (ng/mL ) Analyt e adde d (ng/mL ) 1 2 Metho d Performanc e An ymetho d performance parameter s that are no

t achieve d must b e considere d i n th e evaluatio n ofth e data
. Nonconfomriance t o an yspecifie d parameter s must b e describe d an d discusse d i fth e Technica l Manage r(non GL P study) o rStud yDirecto r(GL P study) choose s t o repor t th e data
. I fcriteri a liste d i n thi s metho d performance sectio n are no t met , maintenance ma

yb e performe d o n th e syste m an d sample s reanalyzed , o rothe raction s take n as appropriate
. Documen t al l action s i n th e ra wdata
. I fdat a are t o b e reporte d whe n perfonnance criteri a hav e no t bee n met , th e dat a must b e footnote d o n table s an d discusse d i n th e tex

t ofth e report
. 12. 1 Syste m Suitabilit y Syste m Suitabilit

ystandard s are no

t a require d componen t o fthi s method
. I frequire d b yprotoco l o rb

yth e technica l manager , a minimu m ofthre e syste m suitabilit ysample s are injecte d a

t th e beginnin g ofeac h analytica l m n prio rt o th e calibratio n curve . Typicall ythes e sample s are a t a concentratio n nea rth e mi d leve l o fth e calibratio n curve an d are repeate d injection s fro m on e autosample rvial
. Th e syste m suitabilit yinjection s mus

t have are a counts wit h a n RS D ofS5% an d a retentio n tim e RS D of^2 % t o b e compliant
. 12. 2 Quantitatio n Calibratio n Curve : Th e coefficien t ofdetenninatio n (r^ ) valu e fo rth e calibratio n curve must b e greate rtha n o r equa l t o 0.990
. Eac h poin t i n th e curve mus t b e withi n ±25 % ofth e theoretica l concentratio n wit h th e exceptio n of th e LLOQ , whic h ma

yb e withi n ±30%
. CCV Performance : Th e calibratio n standard s that are intersperse d throughou t th e analytica l sequence are evaluate d as continuin g calibratio n verification s i n additio n t o bein g par t ofth e calibratio n curve . Th e accurac yof eac h curve poin t must b e withi n 25 % ofth e theoretica l valu e (withi n 30 % fo rlowest curve point)
. Sample s tha t are brackete d b

yCCVs no

t meetin g thes e criteri a must b e reanalyzed
.

ETS-8-044. 0 Pag e 1 0 of1 1 Metho d o fAnalysi s fo rth e Determinatio n o fPerfluorinate d Compound s i n Wate rbyLC/MS/MS ; Direc t Injectio n Analysi s Pag e 8 0 o f8 3

### Documen t ma ybeGlJMM^^id2a'at^'nsr^!ep^fr,tro M day s fro m 03/22/201 1 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0


### Demonstratio n o fSpecificity : Specificit yi s demonstrate d b

ychromatographi cretentio n tim e (withi n 4 % of standard ) an d th e mas s spectra l respons e ofuniqu e ions
. 12. 3 Sensitivit y Th e targete d limi t o fquantitatio n fo ral l analyte s i s 0.02 5 ng/mL
. Th e LOQ fo ran yspecifi canalyt e ma yvar y dependin g o n th e evaluatio n o fappropriat e blank

s an d th e accurac

yofth e low-leve l calibratio n curve points
. Refe r t o Sectio n 1 0 fo radditiona l details
. 12. 4 Accurac y Thi s metho d an d require d qualit ycontro l sample s are designe d t o generat e dat a that are accurat e t o +/-30%
. Sectio n 1 0 contain s additiona l informatio n regardin g th e require d accurac yo flaborator

ycontro l spikes , fiel d matri x spike s an d laborator

ymatri xspikes
. 12. 5 Precisio n Sample s shoul d b e collecte d i n duplicat e i n th e field
. Th e relative percen t difference , RPD , shoul d b e reported
. RP D results greate rtha n 20 % wil l b e flagge d i n th e report , bu t wil l no t b e exclude d fro m reporting
. Th e requiremen t fo rreplicate s exclude s fiel d blank s o rrinse blanks
. Sectio n 1 0 contain s additiona l infonnatio n regardin g th e require d precisio n o flaborator ycontro l spikes
. 1 3 Pollutio n Preventio n an d Wast e Managemen t Wast e generate d whe n performin g thi s metho d wil l b e dispose d o fappropriately
. Th e origina l sample s wil l b e archive d a

t th e 3 M Environmenta l Laborator yi n accordance wit h interna l procedures
. 1 4 Record s Eac h data packag e generate d fo ra stud ymus t includ e al l supportin g informatio n fo rreconstmctio n ofth e data
. Infonnatio n fo rth e dat a packag e must include , bu t i s no t limite d t o th e followin g items : stud yo rprojec t number , sampl e an d standard pre p sheets/records , instrumen t ru n lo g (instmmen t batc h records , instrumen t acquisitio n method , summar

ypages) , instmmen t results files , chromatograms , calibratio n curves , an d dat a calculations
. 

## 1 5 Affecte d Document s None
. 1 6 Revision s Revisio n Numbe rSummar vo fChange s

ETS-8-044. 0 Pag e 1 1 of1 1 Metho d ofAnalysi s fo rth e Determinatio n o fPerfluorinate d Compound s i n Wate rbyLC/MS/MS ; Direc t Injectio n Analysi s Pag e 8 1 o f8 3GLP10-01-02 ; Interi m Repor t 0 9 Analysi s o fPFBS , PFHS , an d PFO S i n Groundwate r Quarterl yGroundwate rSamplin g - Sep t 201 0


### ATTACHMEN TD : DEVIATION(S )

Pag e 82 o f8 3GLPIO-01-02 ; interi m Repor t 0 9 3 M Confidentia l Analysi s o fPFBS , PFHS , and PFO

S i n Groundwate r

_ __

^ „ . QuarterlyGroundwate rSampling-Sept 201 0 

## RECOR D O F DEVIATION/NONCONFORMANC E

/
. identificatio n Stud y/ Projec t No
. Date(s ) o fOccurrence : Documen t Number : GLP10-01-02-0 9 Sequenc e b101108a,g110211a , an d b110315 a ETS-8-044. 0 Deviatio n typ e D SO P D Equipmen t Procedur e IZ I Metho d (Checl < one ) D Protoco l E GP O D Other : //
. Descriptio n (attac h extr a page s a s needed ) 

### Metho d Requirements : (1 ) LC S averag e recover yo f100 % ±20%
. (2 ) Sample/Sanfipl e Duplicat e RP D s20%
. (3 ) FM S recover

yo f100 % ±30%
. 

### Actua l procedure/process : (1 ) LC S averag e recover yfo rth e hig h se t o flinea ran d branche d PFO S wa s 129 % (b l 01108a)
. LC S averag e recover

yfo rth e hig h se t oflinea ran d branche d PFO S wa s 133 % (g1102 1 la)
. (2 ) Th e RP D valu e fo rPFO S fo rDA L G W330 L wa s 24%
. (3 ) Samplin g locatio n DA L G W227 L di d not have a n appropriat e FM S leve l fo rPFOS
. Th e recover yofth e H S fo rPFH S fo rDA L G W330 L wa s 135%
. Th e recover yo fth e H S fo rPFBA , PFHS , an d PFO S fo r DA L G WGRS0 4 wer e <50%
. Th e recover yo fth e H S fo rPFO S fo rDA L G W328 L wa s 69.0 % 

### III
. Action s Take n (suc h a s amendmen t issued , SO P revision , etc. ) 

### Correctiv e Actio n ( D Ye s 0 No ) Reference : Acceptabilityo fth e nonconformin g work : 1 ) Th e linea ran d branche d LC S ha d bee n runnin g 5-15 % highe rwhe n quantitate d agains t a calibratio n curv e comprise d o fprimaril ylinea rPFOS
. Al l LC S were use d i n th e determinatio n o fmetho d uncertainty
. 2 ) Th e RP D valu e fo rPFO S fo rDA L G W330 L wit h a valu e >20 % wil l b e footnote d i n th e dat a tables
. 3 ) Samplin g locatio n DA L G W227 L wil l b e footnote d i n th e repor t a s no t havin g a n appropriat e spike leve l fo rPFOS
. Th e analytica l uncertaintyfo rsamplin g locatio n DA L G W330 L wil l b e adjuste d accordingly
. A5000pp b LM S wa s prepare d o n samplin g locatio n DA L G WGRS0 4 an d me t metho d acceptanc e criteria
. Samplin g locatio n DA L G W328 L L S met metho d acceptanc e criteri a an d wa s th e more appropriat e spik e level
. N o adjustmen t wil l b e mad e t o th e analytica l uncertainty
. Actions : D Haltin g o fWor k D Clien t Notificatio n D Wor k Recal l D Withholdin g o fRepor t 0 Other : Deviation s wil l b e npte d i n fina l report
. 

### Project Lead/PA I Approval : , „ 14-. ^ \ , Date : , , stud yDirecto r(i fGLP) : ^ Q I i
. , :L > Date: ' >j | >7 » i Sponso rApprova l (fo rGL P protoco l deviations) : N ADate : N A/ ' Technica l Reviewe r(optional) : N ADate : N A Laborator yDepartmen t Manage rApproval : Date :

IV
. Authorizatio n t o Riesum e Wor k Wher e haltin g o fwor k

occurred , resumptio n o t wor k mus t lirs t b e approve d b yLaboratoryManagemen t 

### Laborator yDepartmen t Manage rApproval : N ADate : N A Deviatio n No
. (assigned byStudyDirecto ro rTea m Leade rat Ih e en d o fstudyo rproject )

Attachmen t AETS-4-008. 7 Pag e 1 o f1

Documentatio n o fDeviation s an d Contro l o fNonconformin g Testin g Page 8 3 o f8 3