# "#$%&!'(#)*

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**!SO!G29*C!** Bayer CropScience Report Number US 0202

Page 1 of 85

# STATEMENT OF NO DATA CONFIDENTIALITY

**A-!8)2(.!-6!8-06(%*0#(2)(#&!(C!.2%*!6-,!20&!(06-,.2** **#(-0!8-0#2(0*%!(0!#B(C!C#$%&!-0!#B*!D2C(C!-6!**

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**6-,!2))!I$,I-C*C!-#B*,!#B20!8-.I)(208*!5(#B!<=<3>!K** **NX!!"$D.(CC(-0!-6!#B*C*!%2#2!(0!8-.I)(208*!**

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**20&!-#B*,!C#2#$#*!-,!(0!20&!-#B*,!8-$0#,&X! !** Bayer CropScience Report Number US 0202

Page 2 of 85

# GOOD LABORATORY PRACTICE CERTIFICATION

**?--%!)2D-,2#-,&!I,28#(8*!,*;$(,*.*0#C!-6!ZN!H<3!G2,** **#!K[N!(C!0-#!,*;$(,*%!6-,E!20%!%-!0-#!2II)&!**

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**1(C2!"X!],#*9-E!GBX:XE!:>+'!**

**G,(08(I2)!"8(*0#(C#!****! !** Bayer CropScience Report Number US 0202

Page 3 of 85

# ' **]** 

# @! 1(C2!],#*9-E!+2&*,!H,-I"8(*08*!

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# Introduction

**+,-.-/&0()!(C!2!C*)*8#(4*!8-0#28#!D,-2%)*26!B*,D(8(** **%*!#B2#!8-0#,-)C!C-.*!I-C#^*.*,9*0#!5**%CX!!**

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**,2I(%)&!%*9,2%*%!#-!#B*!IB*0-)!6-,.E!8-..-0)&!,*6*,,*%!#-!2C!D,-.-/&0()!VLEOE^%(D,-.-^Z^**

**B&%,-/&D*0`-0(#,()*WX!!'B*!2*,-D(8!C-()!.*#2D-)(C.!** **C#$%&!6-,!D,-.-/&0()!-8#20-2#*!CB-5C!2!**

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***C#*,!6-,.E!D$#!(C!0-#!,*9(C#*,*%!(0!#B*!U0(#*%!"#2** **#*C!20%!(C!0-#!,*)*420#!#-!UX"X!*04(,-0.*0#2)!**

***/I-C$,*CX!**

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**IB*0-)X!!3*C$)#C!6,-.!D,-.-/&0()!-8#20-2#*!C#$%(*C!** **CB-5!CB-,#!B2)6^)(6*!#(.*C!(0!D-#B!52#*,!**

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**%(66*,*08*C!(0!C*0C(#(4(#&!%*I*0%(09!$I-0!#B*!6-,.!** **-6!D,-.-/&0()!#-!5B(8B!#B*&!2,*!*/I-C*%!V#B*!**

**IB*0-)!6-,.!(C!)*CC!#-/(8!#B20!#B*!*C#*,!6-,.C!6-,!** **C-.*!#2/2WX!**

**=0!.$8B!-6!#B*!I**,^,*4(*5*%!)(#*,2#$,*E!D,-.-/&0()** **!(C!)(C#*%!2C!#B*!#*C#!.2#*,(2)!5B(8B!.$C#!D*!**

**2CC$.*%!#-!D*!#B*!IB*0-)!6-,.!$0)*CC!-#B*,5(C*!C#2#** ***%!-,!C$II-,#*%!D&!202)&#(82)!8-06(,.2#(-0X!!** 

# 7 **e ' 7 3 A > 1 ! !** 

# Y **7 Y ] 3 > A : U Y** Bayer CropScience Report Number US 0202

Page 4 of 85

# >C!.*0#(-0*%!2D-4*E!*/I-C$,*C!8)2(.(09!#-!D*!5(#B!2 **0!*C#*,!6-,.!.$C#!D*!8)-C*)&!*/2.(0*%!#-!**

**8-06(,.!#B2#!#B*!C#$%&!%*C(90!C$II-,#C!#B*!8)2(.X!**



# Literature Search

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**3*C-$,8*C!>DC#,28#CE!>;$2#(8!"8(*08*!h!<(CB*,(*C!>D** **C#,28#CE!A'="E!?*->,8B(4*E!?7]+>"7E!**

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**i.28,-IB&#*j!-,!iI*,(IB&#-0j!-,!iD(,%jX!!"&C#*.!2)9** **-,(#B.C!5*,*!$C*%!#-!)(.(#!#B*!,*C$)#C!#-!**

**.2#*,(2)C!I$D)(CB*%!(0!709)(CBE!#-!,*.-4*!2C!.20&!%** **$I)(82#*!8(#2#(-0C!2C!I-CC(D)*E!20%!#-!C-,#!#B*!**

**,*.2(0(09!,*8-,%C!(0!,*4*,C*!8B,-0-)-9(82)!-,%*,!V.** **-C#!,*8*0#!6(,C#WX!!"*I2,2#*!C*2,8B*C!5*,*!**

**8-0%$8#*%!(0!*28B!%2#2D2C*!82#*9-,&E!5B(8B!,*C$)#*%** **!(0!C-.*!%$I)(82#(-0!-6!,*C$)#CX!!** Bayer CropScience Report Number US 0202

Page 5 of 85

# 3*C$)#C!6,-.!#B*C*!C*2,8B*C!5*,*!,*4(*5*%!20%!I2I*,C!8)*2,)&!$0,*)2#*%!#-!D,-.-/&0()!

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# Review Criteria

**]08*!-D#2(0*%E!#B*!I2I*,C!5*,*!m$%9*%!288-,%(09!#-!** **#B*!]66(8*!-6!G*C#(8(%*!G,-9,2.Cn!V]GGW!**

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vitro **!C#$%(*CW!**

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# \1H **ON\** 

# 1: **ON** 

# W!V(6!0-#!*/I)(8(#)&!,*I-,#*%!(0!#*/#E!

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**2II,-I,(2#*WX!** Bayer CropScience Report Number US 0202

Page 6 of 85

# >%%(#(-02))&E!#B*!6-))-5(09!628#-,C!5*,*!2)C-!(%*0# **(6(*%!6-,!*28B!I2I*,E!#-!6$,#B*,!m$%9*!(#C!**

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# Aquatic Receptors

Algae and Plants

**'B*!)(#*,2#$,*!C*2,8B!%*C8,(D*%!2D-4*!,*#$,0*%!2!#-** **#2)!-6!KS!I2I*,C!8-08*,0(09!#B*!#-/(8(#&!-6!**

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**#B*(,!,*I-,#*%!*0%I-(0#C**

**X!**

Acceptable Studies



# ?2,#*0!VKRRNW!%*#*,.(0*%!#B*!#-/(8(#&!-6!#*8B0(82)! **D,-.-/&0()!#-!** Selenastrum capricornutum

**20%!** Chorella vulgaris **!$C(09!]7H:!.*#B-%-)-9&E!,*I-,#(09!2!R[^B-$,!A]>7H!** **-6!K!.9\1X!!**

**?,-5#B!52C!,*%$8*%!D&!2II,-/(.2#*)&!LOo!2#!KN!.9\1!** **D,-.-/&0()!20%!52C!8-.I)*#*)&!**

**(0B(D(#*%!2#!KNN!.9\1!D,-.-/&0()X!!=0!2!C*8-0%!,*6*** **,*08*E!6-$,!2)92*!CI*8(*C!V** S. quadricauda

**E!** S.

capricornutum

**E!** Nitzschia **!CIXE!** Cyclotella meneghiniana **W!20%!6(4*!8&20-D28#*,(2!CI*8(*C!**

**V** Microcystis aeruginosa, Oscillatoria **!CIXE!** Pseudanabaena **!CIXE!** Anabaena inaequalis **20%!**

Aphanizomenon flos-aquae **W!5*,*!*/I-C*%!#-!NXMS!.9\1!#*8B0(82)!D,-.-/&0()!D$** **#&,2#*!*C#*,!6-,!**

**MM!B-$,CX!!A-!C(90(6(820#!9,-5#B!(0B(D(#(-0!52C!-DC** ***,4*%!6-,!20&!#*C#*%!CI*8(*C!V** Nitzschia **CIX!**

**2)92*!9,-5#B!52C!(08,*2C*%!ZNo!D&!#B(C!*/I-C$,*Wd!8** **-0C*;$*0#)&E!#B(C!8-08*0#,2#(-0C!820!D*!** Bayer CropScience Report Number US 0202

Page 7 of 85

# (0#*,I,*#*%!2C!20!$0D-$0%*%!A]>7H!VG*#*,C-0!*#!2)X! **KRRZWX!!_-5*4*,E!D,-.-/&0()!D$#&,2#*!(C!**

**0-#!,*9(C#*,*%!6-,!$C*!(0!#B*!U0(#*%!"#2#*CX!**

**H$))(.-,*!VKRPOW!*42)$2#*%!#B*!#-/(8(#&!-6!#*8B0(82** **)^9,2%*!D,-.-/&0()!#-!KL!CI*8(*C!-6!**

**HB)-,-IB&8*2*!2)92*X!!>)#B-$9B!8-08*0#,2#(-0C!$I!#-** **!KN!.9\1!5*,*!#*C#*%E!-0)&!** Hormidium

barlowi **!*/B(D(#*%!2%4*,C*!*66*8#C!(0!,*CI-0C*!#-!*/I-C$,*C** **X!!'B*!7H** **ON** 

# !V9,-5#BW!6-,!#B(C!CI*8(*C!

**52C!,*I-,#*%!#-!D*!D*#5**0!NXO!20%!M!.9\1!D,-.-/&0(** **)E!5B()*!#B*!)-5*C#!8-08*0#,2#(-0!2#!5B(8B!**

**9,-5#B!(0B(D(#(-0!-88$,,*%!52C!O!.9\1!D,-.-/&0()X!!** **"(.()2,)&E!"#^12$,*0#!*#!2)X!VKRRMW!**

**%*#*,.(0*%!#B*!#-/(8(#&!-6!#*8B0(82)^9,2%*!D,-.-/&0** **()!#-!2)92*!** S. capricornutum **!#B,-$9B!**

**R[^B-$,!D(-2CC2&C!8-0%$8#*%!(0!*(#B*,!.(8,-I)2#*!-,** **!6)2CT!*/I-C$,*!C&C#*.CX!!'B*!,*C$)#(09!**

**7H ON**  

# C!5*,*!%*#*,.(0*%!#-!D*!MXR!.9!D,-.-/&0()\1!(0!#B*! **6)2CT!C&C#*.!20%!LXZ!.9!D,-.-/&0()\1!**

**(0!#B*!.(8,-I)2#*!*/I-C$,*CX!!Y2!*#!2)X!VMNNMW!,*I-** **,#*%!2!8-0C(%*,2D)&!B(9B*,!7H** **ON** 

# !-6!SRXK!.9!

**D,-.-/&0()\1!6-,!#B*!2)92*!CI*8(*C!** C. vulgaris

**X!**

**'B*!#-/(8(#&!-6!D,-.-/&0()!-8#20-2#*!#-!6(4*!CI*8(*** **C!-6!2)92*!V** Pseudokirchneriella subcapitata

**b** S. capricornutum **cE** S. quadricauda

**E!** Scenedesmus obliquus

**E!** Chlorella vulgaris **E!20%!** Chlorella

pyrenoidosa **W!52C!%*#*,.(0*%!D&!Y2!*#!2)X!VMNNPWX!!12D-,2#-,&!8$)#$,*C!5*,*!*/I-C*%!6-,!**

**R[!B-$,C!#-!#*8B0(82)!D,-.-/&0()!-8#20-2#*!VR[o!I$,(#&WE!20%!)(0*2,!,*9,*CC(-0!202)&C(C!**

**9*0*,2#*%!7H** **ON** 

# !42)$*C!D*#5**0!ZXZL!.9\1!V P. subcapitata **!b** S. capricornutum **cW!20%!PXOZ!.9\1!**

**V** S. obliquus **WX!!** C. pyrenoidosa **E!B-5*4*,E!52C!C(90(6(820#)&!.-,*!#-)*,20#!#-!D,-.-** **/&0()^**

**-8#20-2#*!#B20!#B*!-#B*,!CI*8(*C!#*C#*%E!5(#B!2!82)8$)2#*%!7H** **ON** 

# !-6!LMZXOO!.9\1X!!A]>7H!42)$*C!

**6-,!#B*!6-$,!.-C#!C*0C(#(4*!2)92*!CI*8(*C!,209*%!6,** **-.!NXM!.9\1!V** S. quadricauda **W!#-!K!.9\1!**

**V** S. obliquus **WX!!=0!2!C*I2,2#*!I2I*,E!Y2!20%!1(209!VMNNKW!,*I-,#*%!D,-.-/&0()!7H** **ON** 

# !42)$*C!6-,!

C. pyrenoidosa **!20%!** S. obliquus **!D2C*%!-0!(0B(D(#(-0!-6!IB-#-C&0#B*C(CX!!H2)8$)2#*%** **!R[^B-$,!7H ON**  

# 42)$*C!6-,!IB-#-C&0#B*C(C!(0B(D(#(-0!5*,*!ZXZ!.9\1! **6-,!** C. pyrenoidosa **20%!OLXZ!.9\1!6-,!**

S. obliquus **X!!<2(,8B()%!*#!2)X!VKRRPW!,*I-,#*%!2!R[^B-$,!D,-.-** **/&0()!A]>7HE!1]>7HE!20%!7H ON** 



# 6-,! S. capricornutum **!-6!LEKMO!p9\1E![EMON!p9\1E!20%!PEP[M!p9\1E!,*CI*8#** **(4*)&X!!>)#B-$9B!#*C#!**

**8-08*0#,2#(-0C!5*,*!0-#!CI*8(6(*%E!#B*!8-06(%*08*!(** **0#*,42)C!2CC-8(2#*%!5(#B!#B*!,*I-,#*%!**

***0%I-(0#C!(0%(82#*!#B2#!#*C#!8-08*0#,2#(-0C!5*,*!2%** ***;$2#*!6-,!%*#*,.(02#(-0!-6!*0%I-(0#CX!**

**_*CC!VKRSNW!%*#*,.(0*%!#B*!ZS^B-$,!#-/(8(#&!-6!D,-.** **-/&0()!#-!!** Chlamydomonas eugametos

**2)92*X!!A-!*66*8#C!-0!9,-5#B!5*,*!-DC*,4*%!2#!2!D,-** **.-/&0()!8-08*0#,2#(-0!-6!K** ! **KN**

! **O** 

# !Y!

**V2II,-/(.2#*)&!MXS!.9\1E!2CC$.(09!2!.-)*8$)2,!5*(9B** **#!-6!MP[XRWd!B-5*4*,E!8-.I)*#*!(0B(D(#(-0!**

**52C!-DC*,4*%!2#!#B*!0*/#!B(9B*C#!8-08*0#,2#(-0E!K** ! **KN**

! **Z** 

# !Y!VMPXP!.9\1WX!!>!8-08$,,*0#!

**(04*C#(92#(-0!-6!#B*!#-/(8!*66*8#C!-6!:Y"]^B*,D(8(%** ***!.(/#$,*C!(0%(82#*%!#B2#!#B*!C-)4*0#!**

**C(90(6(820#)&!*0B208*%!#B*!#-/(8(#&!-6!2)28B)-,E!D$** **#!B2%!0-!*66*8#!-0!I2,2;$2#!#-/(8(#&!#-!** C.

eugamentos **X!!'B*!*66*8#!-6!:Y"]!-0!D,-.-/&0()!#-/(8(#&!52C!0-** **#!#*C#*%!V_*CC!KRSNWX!!=0!**

**2%%(#(-0E!#B*!2)92*!CI*8(*C!** Chlamydomonas moewusii **!V** C. eugametos **W!52C!%*#*,.(0*%!#-!D*!**

**#-)*,20#!#-!#*8B0(82)^9,2%*!D,-.-/&0()!(0!L^%2&!,*I** **,-%$8#(4*!20%!P^%2&!9,-5#B!D(-2CC2&C!VH2(0!**

**20%!H2(0!KRSLWX!!'B*!B(9B*C#!8-08*0#,2#(-0!#*C#*%E!SN!pY!V2II,-/(.2#*)&!MMXM!.9\1W!52C!**

**%*#*,.(0*%!#-!B24*!0-!*66*8#!-0!#B(C!CI*8(*Cn!9,-5#** **B!20%!,*I,-%$8#(-0X!!"(.()2,)&E!2!P^%2&!**

***/I-C$,*!#-!LXOP** ! **KN**

! **O** 

# !Y!D,-.-/&0()!VRXSR!.9!D,-.-/&0()\1E!2CC$.(09!2!.-) ***8$)2,!5*(9B#!-6!**

**MP[XRW!52C!%*#*,.(0*%!#-!B24*!0-!*66*8#!-0!#B*!9,-5** **#B!-6!2)92*!CI*8(*C!** C. pyrenoidosa **!20%!**

Stichococcus bacillaris **!2#!2!I_!-6![XOd!#B*!A]>7H!6-,!20!2%%(#(-02)!CI*8(*C!V** Chlamydomonas

globosa **W!52C!(0#*,I,*#*%!#-!D*!MXLS** ! **KN**

! **O** 

# YE!-,![X[!.9!D,-.-/&0()\1!V<)*#8B*,!*#!2)X!KRPNWX!! **'B*!**

**2$#B-,C!2)C-!,*I-,#*%!#B2#!#B*!B*,D(8(%*!52C!.-,*!#** **-/(8!2#!2!I_!-6!OE!#B20!2#![XO!-,!SX!** Bayer CropScience Report Number US 0202

Page 8 of 85

# >C!2!8-.I-0*0#!-6!)2,42)!-&C#*,!V Crassostrea gigas **W!#-/(8(#&!#*C#CE!_(C!20%!"*2.20!VKRRLW!2)C-!**

**%*#*,.(0*%!#B*!#-/(8(#&!-6!D,-.-/&0()!#-!#B*!.2,(0*** **!2)92*!CI*8(*C!$#()(`*%!2C!-&C#*,!%(*#E!**

Isochrysis galbana **!20%!** Chaetoceros calcitrans **X!!'B*!9,-5#B!-6!** I. galbana **52C!C(90(6(820#)&!**

**266*8#*%!D&!D,-.-/&0()!*/I-C$,*E!5(#B!2!MK^%2&!7H** **ON** 

# !-6!PXO!.9\1d! C. calcitrans **!52C!$0266*8#*%!**

**D&!2))!8-08*0#,2#(-0C!-6!D,-.-/&0()!#*C#*%E!9(4(09!** **20!$0D-$0%*%!A]>7H!-6!KN!.9\1X!!**

Unacceptable Studies



# 'B*!,*.2(0(09!C(/!2)92)!#-/(8(#&!I2I*,C!62()*%!#-!. ****#!]GG!8,(#*,(2!6-,!2!0$.D*,!-6!,*2C-0CX!!**

**'B,**!5*,*!,*m*8#*%!D*82$C*!2)#B-$9B!#B*!2$#B-,C!,*I-,#*%!*0%I-(0#CE!#B*!#*C#!8-08*0#,2#(-0C!20%!**

**8-06(%*08*!(0#*,42)C!2CC-8(2#*%!5(#B!#B*!,*I-,#*%!*** **0%I-(0#C!5*,*!0-#!I,*C*0#*%!VY2!*#!2)X!MNNLE!**

**MNN[d!Q2)CB!*#!2)X!KRSPWX!!Y2!*#!2)X!VMNN[W!%*#*,.(** **0*%!#B*!#-/(8(#&!-6!#*8B0(82)!9,2%*!**

**D,-.-/&0()!#-!** S. capricornutum **!20%!82)8$)2#*%!2!R[^B-$,!7H** **ON** 

# !-6!ZXML!.9\1X!!'B*!R[^B-$,!

**D,-.-/&0()!7H ON**  

# !6-,!2!,*)2#*%!CI*8(*CE! S. quadricauda **E!52C!,*I-,#*%!2C!LXP!.9\1!VY2!*#!2)X!**

**MNNLWX!!'B*!PM^B-$,!D,-.-/&0()!7H** **ON** 

# !42)$*C!6-,!.2,(0*!%(2#-.!CI*8(*C!" keletonema costatum

**20%!** Thalassiosira pseudonana **!5*,*!%*#*,.(0*%!#-!D*!NXS[**

! **MXO!.9\1!20%!KXM**

! **OXN!.9\1E!**

**,*CI*8#(4*)&E!%*I*0%(09!-0!#B*!#&I*!-6!C2)#52#*,!VQ** **2)CB!*#!2)X!KRSPWX!!=0!2%%(#(-0E!#B*!R[^B-$,!**

**7H ON**  

# !6-,! Chlorella **CIX!2)92*!,209*%!D*#5**0!LXK!#-!KN!.9!D,-.-/&0()\1X !!!!** 

**=0!2))!82C*CE!#B*C*!*0%I-(0#C!5*,*!C#2#(C#(82))&!%*** **,(4*%!6,-.!#-/(8(#&!%2#2!6,-.!D(-2CC2&C!**

**8-0%$8#*%!5(#B!$0T0-50!D,-.-/&0()!8-08*0#,2#(-0CX!!** **"$DC*;$*0#)&E!(#!8200-#!D*!%*#*,.(0*%!(6!**

**#B*!C*)*8#*%!#*C#!8-08*0#,2#(-0C!5*,*!2II,-I,(2#*!6** **-,!#B(C!C#2#(C#(82)!$C*X!**

**'B,**!-#B*,!2)92*!#-/(8(#&!I2I*,C!5*,*!%*#*,.(0*%!#** **-!D*!$0288*I#2D)*!$0%*,!]GG!8,(#*,(2X!!q())2!**

***#!2)X!VMNNLW!,*I-,#*%!2!D,-.-/&0()!R[^B-$,!7H** **ON** 

# !-6!S!.9\1!6-,!20!$0(%*0#(6(*%!2)92*!CI*8(*CX!!

**_-5*4*,E!#B(C!I2I*,!52C!0-#!#B*!I,(.2,&!C-$,8*!-6!#** **B*!%2#2X!!F,2#T&!20%!Q2,,*0!VKRPKW!**

**I,-4(%*C!-0)&!2!;$2)(#2#(4*!(0#*,I,*#2#(-0!-6!D,-.-** **/&0()nC!*66*8#C!-0!2)92*!CI*8(*C!** C.

pyrenoidosa **X!!1*CC!#B20!ONo!9,-5#B!(0B(D(#(-0!52C!,*I-,#*%!2#!** **K!II.!D,-.-/&0()!20%!9,*2#*,!**

**#B20!ONo!9,-5#B!(0B(D(#(-0!52C!,*I-,#*%!2#!KN!II.E!D$#!#B*!I,*8(C*!)*4*)!-6** **!9,-5#B!(0B(D(#(-0!2#!**

***28B!8-08*0#,2#(-0!52C!0-#!9(4*0X!'B*!,*.2(0(09!I2I** ***,!V<2(,8B()%!*#!2)XKRRRW!,*I-,#*%!%2#2!#B2#!**

**5*,*!-,(9(02))&!I,*C*0#*%!(0!<2(,8B()%!*#!2)X!KRRPX**

**]0)&!#5-!I2I*,C!8-08*,0(09!#B*!#-/(8(#&!-6!D,-.-/&0** **()!#-!2;$2#(8!.28,-IB&#*C!5*,*!(%*0#(6(*%!**

**%$,(09!#B*!)(#*,2#$,*!C*2,8BE!-0*!-6!5B(8B!.*#!]GG!** **8,(#*,(2!6-,!288*I#2D()(#&X!!G*#*,C-0!*#!2)X!**

**VKRRZW!*/I-C*%!** Lemna minor **!VL^6,-0%!C#29*W!#-!#*8B0(82)!D,-.-/&0()!D$#&,2#*!2** **#!NXMS!.9\1!6-,!**

**P!%2&CX!!?,-5#B!52C!$0266*8#*%!2#!NXMS!.9!D,-.-/&0()!D$#&,2#*\1E!20%!8-0C*;$*0#)&E!#B(C!**

**8-08*0#,2#(-0!820!D*!(0#*,I,*#*%!2C!#B*!$0D-$0%*%!A** **]>7H!6-,!** L. minor **9,-5#BX!!>C!I,*4(-$C)&!**

**0-#*%E!#B*!D$#&,2#*!6-,.!(C!0-#!,*9(C#*,*%!6-,!$C*!** **(0!#B*!U0(#*%!"#2#*CX!!>!C*8-0%!I2I*,!2)C-!**

**(04*C#(92#*%!#B*!#-/(8(#&!-6!#*8B0(82)!D,-.-/&0()!#-!KM^6,-0%!)(6*!C#29*!** L. minor **!.28,-IB&#*CE!**

**,*I-,#(09!R[^B-$,!1]>7H!20%!7H** **ON** 

# !42)$*C!-6!LEKMO!20%!SEN[O!p9\1E!,*CI*8#(4*)&!V<2(, **8B()%!*#!**

**2)X!KRRPWX!!_-5*4*,E!#B*!2$#B-,C!%(%!0-#!,*I-,#!#B*** **!#*C#!8-08*0#,2#(-0C!$C*%!#-!C#2#(C#(82))&!%*,(4*!**

**#B*C*!*0%I-(0#C!20%!2C!C$8B!#B*!42)(%(#&!-6!#B*!,*C$)#C!8200-#!D*!m$%9*%X!**

**=0!8-08)$C(-0E!D2C*%!-0!#B*!242()2D)*!)(#*,2#$,*E!#** **B*!*66*8#!8-08*0#,2#(-0C!6-,!#B*!.-C#!C*0C(#(4*!**

**2)92*!20%!2;$2#(8!I)20#!CI*8(*C!#*C#*%!,209*C!6,-.!** **2II,-/(.2#*)&!K!#-!KN!.9\1!6-,!#*8B0(82)!**

**D,-.-/&0()X!!A-0*!-6!#B*!(%*0#(6(*%!I2I*,C!#*C#*%!#** **B*!#-/(8(#&!-6!D,-.-/&0()!6-,.$)2#(-0C!#-!** Bayer CropScience Report Number US 0202

Page 9 of 85

# 2;$2#(8!I)20#C!20%!2)92*E!C-!(#!(C!$08)*2,!(6!6-,.$)2#*%!I,-%$8#C!5-$)%!*)(8(#!C(90(6(820#)&!

**%(66*,*0#!,*C$)#CX!**



# Aquatic Invertebrates

**<-$,!I2I*,C!8-0#2(0(09!(06-,.2#(-0!,*92,%(09!D,-.-/&0()!#-/(8(#&!#-!2;$2#(8!(0** **4*,#*D,2#*C!5*,*!**

**(%*0#(6(*%!#B-$9B!#B*!)(#*,2#$,*!C*2,8Bd!#B,**!-6!#** **B*C*!5*,*!m$%9*%!2C!288*I#2D)*!288-,%(09!#-!**

**]GG!8,(#*,(2X!**

**+$B)!*#!2)X!VKRSRW!*/I-C*%!6-$,#B!(0C#2,!** Chironomus riparius **!)2,42*!#-!Y7Z!+,-.(02)!VLZXRo!**

**D,-.-/&0()W!6-,!ZS!B-$,CX!!'B*!82)8$)2#*%!MZ^B-$,!20%!ZS^B-$,!7H** **ON!** 

# V(..-D()(`2#(-0W!42)$*C!

**5*,*!%*#*,.(0*%!#-!D*!MXLO!.9\1!20%!KXR!.9\1E!,*CI*** **8#(4*)&X!!>)#B-$9B!CI*8(6(8!#*C#!**

**8-08*0#,2#(-0C!5*,*!0-#!,*I-,#*%E!#B*!C.2))!8-06(%*** **08*!(0#*,42)C!6-,!#B*C*!*0%I-(0#C!(0%(82#*!#B*!**

**$C*!-6!C$(#2D)*!8-08*0#,2#(-0CX!!=0!KRRLE!+$B)!*#!2** **)X!VKRRLW!I$D)(CB*%!-0!#B*!8B,-0(8!#-/(8(#&!-6!**

**#B*!D,-.-/&0()!6-,.$)2#(-0!+$8#,()** **r** 

# !#-!#B*!8)2%-8*,20! Daphnia magna **X!!A*-02#*C!aMZ!B-$,C!**

**-)%!5*,*!*/I-C*%!#-!*(#B*,!8-0#(0$-$C!+$8#,()** **r** 

# !8-08*0#,2#(-0C!-6!OE!KNE!MNE!ZNE!SN!p9\1E!-,!#-!

**%2()&!I$)C*C!#B2#!924*!MZ^B-$,!24*,29*!8-08*0#,2#(-** **0C!-6!#B*!C2.*!.290(#$%*X!!'B*!A]>7HE!**

**1]>7HE!20%!9*-.*#,(8!.*20!.2/(.$.!288*I#2D)*!#-/(82** **0#!8-08*0#,2#(-0!VY>'HW!42)$*C!6-,!**

**8-0#(0$-$C!*/I-C$,*!D2C*%!-0!9,-5#BE!,*I,-%$8#(-0E!** **20%!I-I$)2#(-0!9,-5#B!*0%I-(0#C!20%!5*,*!**

**MNE!ZNE!20%!MS!p9\1E!,*CI*8#(4*)&X!=0#*,.(##*0#!*/I** **-C$,*C!5*,*!-DC*,4*%!#-!D*!.-,*!#-/(8!#-!**

**%2IB0(%C!#B20!5*,*!8-0C#20#!*/I-C$,*CE!5(#B!,*I-,#*%!A]>7HE!1]>7HE!20%!9*-.*#,(8!.*20!**

**Y>'H!42)$*C!-6!KNE!MNE!20%!KZ!p9\1E!,*CI*8#(4*)&X!!** **<(02))&E!#B*!#-/(8(#&!-6!D,-.-/&0()!#-!)2,42*!**

**-6!#B*!.2,(0*!-&C#*,!** Crassostrea gigas **!52C!*42)$2#*%!D&!_(C!20%!"*2.20!VKRRLWX!!'B*!R^%2&**

***/I-C$,*!52C!(0(#(2#*%!5(#B!#B*!*.D,&-0(8!C#29*E!20** **%!#*C#!8-08*0#,2#(-0C!,209*%!D*#5**0!NXNMO!**

**20%KN!.9\1X!!'B*!,*I-,#*%!R^%2&!1H** **ON** 

# !52C!PXN!.9\1E!5B()*!#B*!7H **KN** 

# !42)$*!6-,!CB*))!%*I-C(#(-0!

**52C!82)8$)2#*%!#-!D*!NXSN!.9\1!D,-.-/&0()X!!q())2!*** **#!2)X!VMNNLW!2)C-!,*I-,#*%!2!ZS^B-$,!1H** **ON** 

# !6-,!

D. magna **!-6!KMXO!.9!D,-.-/&0()\1d!B-5*4*,E!#B(C!I2I*,!52C!0** **-#!#B*!I,(.2,&!C-$,8*!-6!#B*!%2#2!**

**20%!C-!52C!m$%9*%!$0288*I#2D)*!D&!]GG!8,(#*,(2X!**

**+,-.-/&0()!*)(8(#C!#-/(8!*66*8#C!(0!2;$2#(8!(04*,#*** **D,2#*C!2#!*/I-C$,*C!-6!2II,-/(.2#*)&!**

**K**

! **KO!.9\1X!!"B*))!%*I-C(#(-0!(0!#B*!.2,(0*!-&C#*,!** C. gigas **!52C!#B*!.-C#!C*0C(#(4*!*0%I-(0#!**

**I,*C*0#*%!(0!#B*!2D-4*!,*6*,*08*CE!5B()*!%2IB0(%C!2** **II*2,!#-!D*!,*)2#(4*)&!#-)*,20#!-6!*/I-C$,*X!!**

**'*C#(09!5(#B!#B*!6-,.$)2#*%!I,-%$8#!Y7Z!+,-.(02)!(0** **%(82#*C!#B2#!6-,.$)2#(-0!#-/(8(#&!#-!**

**2;$2#(8!(04*,#*D,2#*C!(C!)(T*)&!#-!D*!C(.()2,!#-!#B** **2#!-6!#B*!#*8B0(82)!9,2%*!.2#*,(2)X!**



# Fish

**'B,**!I$D)(82))&^242()2D)*!,*6*,*08*C!,*92,%(09!D,-** **.-/&0()!#-/(8(#&!#-!6(CB!5*,*!(%*0#(6(*%!**

**%$,(09!#B*!)(#*,2#$,*!C*2,8BE!-6!5B(8B!#5-!5*,*!m$%** **9*%!#-!D*!288*I#2D)*!$0%*,!]GG!8,(#*,(2X!**

**'B*!288*I#2D)*!,*6*,*08*C!8-.I,(C*%!C*I2,2#*!*0#,(*** **C!(0!#5-!%(66*,*0#!4-)$.*C!-6!#B*!D--T!** Acute

Toxicities of Organic Chemicals to Fathead Minnows ( **G(.*IB2)*C!I,-.*)2C**

) **X!!=0!#B*!KRSZ!**

**4-)$.*E!LN^%2&^-)%!62#B*2%!.(00-5!5*,*!*/I-C*%!#-!M** **XZPE!LXSNE!OXSOE!RXNE!20%!KLXS!.9!**

**#*8B0(82)^9,2%*!D,-.-/&0()\1!6-,!R[!B-$,CX!!:2()&!2** **02)&#(82)!4*,(6(82#(-0!-6!#B*C*!#*C#!**

**8-08*0#,2#(-0C!52C!I*,6-,.*%X!!'B*!R[^B-$,!1H** **ON** 

# !52C!82)8$)2#*%!#-!D*!KKXO!.9\1!V+,--T*!*#!2)X!

**KRSZWX!!"(.()2,)&E!?*(9*,!*#!2)X!VKRSSW!*/I-C*%!MP^** **!#-!ZZ^%2&!-)%!62#B*2%!.(00-5!#-!#*8B0(82)!** Bayer CropScience Report Number US 0202

Page 10 of 85

# 9,2%*!D,-.-/&0()!6-,!R[!B-$,CX!!'*C#!8-08*0#,2#(-0C!VZXZLE![XSME!KNXOE!K[XKE!20%!MZXS!.9\1W!

**5*,*!202)&#(82))&!4*,(6(*%!%2()&X!!'B*!2$#B-,C!%*#*,.(0*%!#B*!R[^B-$,!D,-.-/&0()!1H** **ON** 

# !6-,!

**62#B*2%!.(00-5!#-!D*!KLXS!.9\1E!20%!2)C-!0-#*%!C(90** **(6(820#!D*B24(-,!*66*8#C!2CC-8(2#*%!5(#B!**

***/I-C$,*E!(08)$%(09!)-CC!-6!C8B--)(09!D*B24(-,E!B&I** ***,28#(4(#&E!20%!(08,*2C*%!,*CI(,2#(-0X!!A-#!**

**-0)&!%(%!#B*C*!,*6*,*08*C!.**#!]GG!8,(#*,(2E!#B*&!*** **/8**%*%!#B*C*!8,(#*,(2!D&!.*2C$,(09!28#$2)!**

**#*C#!8-08*0#,2#(-0C!%2()&X!**

**q())2!*#!2)X!VMNNLW!,*I-,#*%!2!R[^B-$,!D,-.-/&0()!1** **H ON**  

# !-6!NXKO!.9\1!6-,!20!$0(%*0#(6(*%!6(CB!

**CI*8(*CX!!_-5*4*,E!#B(C!I2I*,!52C!0-#!#B*!I,(.2,&!C** **-$,8*!-6!%2#2E!D$#!(0C#*2%!2##,(D$#*%!#B(C!**

***0%I-(0#!42)$*!#-!20!=#2)(20!,*9$)2#-,&!%-8$.*0#X!!** **>C!C$8BE!#B(C!42)$*!8-$)%!0-#!D*!4*,(6(*%!20%!**

**(C!0-#!(0!288-,%208*!5(#B!]GG!8,(#*,(2X!**

**3*C$)#C!6,-.!*/I*,(.*0#2#(-0!5(#B!62#B*2%!.(00-5C!C** **$99*C#C!#B2#!D,-.-/&0()!(C!28$#*)&!)*#B2)!**

**#-!6(CB!2#!8-08*0#,2#(-0C!-6!2II,-/(.2#*)&!KN!.9\1!** **V*X9XE!C(.()2,!#-!#B2#!-6!2)92*E!2;$2#(8!I)20#CE!**

**20%!2;$2#(8!(04*,#*D,2#*CWX!!'B(C!(06-,.2#(-0!C$99*C#C!#B2#!D,-.-/&0()!820!D*!8-0C(%*,*%!2C!**

**.-%*,2#*)&!#-/(8!#-!.-C#!2;$2#(8!CI*8(*CX!!!**



# Terrestrial Receptors

Plants

**+,-.-/&0()!(C!2!B(9B)&!C*)*8#(4*!B*,D(8(%*E!#2,9*#(** **09!D,-2%)*26!I)20#C!20%!(C!)2,9*)&!(0*66*8#(4*!**

**-0!9,2CC*CX!!>!C$,4*&!-6!#B*!)(#*,2#$,*!8-06(,.C!#B** **2#!5B()*!C-.*!I)20#C!2,*!;$(#*!C*0C(#(4*!#-!(#C!**

***66*8#CE!-#B*,C!2,*!*/#,*.*)&!#-)*,20#X!!'B*,*6-,*E** **!#B*,*!(C!2!)2,9*!,209*!(0!C*0C(#(4(#&!2.-09!**

**I)20#!CI*8(*CX!!>C!(0!#B*!2;$2#(8!*8-C&C#*.E!#B*!B2** **)6^)(4*C!-6!#B*!-8#20-2#*!20%!B*I#20-2#*!*C#*,C!**

**2,*!4*,&!CB-,#!(0!#*,,*C#,(2)!C&C#*.CX!!74*0!(6!-0*** **!-6!#B*!*C#*,!6-,.C!(C!8-06(,.*%!2C!#B*!#*C#!**

**C$DC#208*E!(#!5())!,2I(%)&!%*9,2%*!#-!#B*!IB*0-)!6-** **,.X!!>!8-$I)*!-6!%2&C!26#*,!C#$%&!(0(#(2#(-0E!**

***/I-C$,*!(C!)2,9*)&!6,-.!#B*!IB*0-)!6-,.E!$0)*CC!.$** **)#(I)*!2II)(82#(-0C!2,*!$C*%X!!<$,#B*,.-,*E!**

**#B*!#*C#!.2#*,(2)!.2&!%*9,2%*!#-!#B*!IB*0-)!6-,.!(0** **!C#-8T!C-)$#(-0C!B*)%!6-,!)-09*,!#B20!MZ!B-$,C!**

**I,(-,!#-!$C*X!**

Acceptable Studies



# 'B(,#&!-0*!I2I*,C!I*,#2(0(09!#-!#B*!#-/(8(#&!-6!D,- **.-/&0()!#-!0-0^#2,9*#!#*,,*C#,(2)!I)20#!CI*8(*C!**

**5*,*!(%*0#(6(*%!#B,-$9B!#B*!)(#*,2#$,*!C*2,8BX!!G2I** ***,C!*/2.(0(09!#B*!,*)2#(4*!C$C8*I#(D()(#(*C!-6!**

**%(66*,*0#!8,-I!8$)#(42,C!#-!D,-.-/&0()!-,!,*6*,*08*** **C!8-08*,0(09!8-0#,-)!-6!5**%!CI*8(*C!2,*!0-#!**

**,*4(*5*%!(0!#B(C!C*8#(-0X!!]6!#B*!LN!(%*0#(6(*%!I2I** ***,CE!K[!5*,*!%*#*,.(0*%!#-!D*!288*I#2D)*!$0%*,!**

**]GG!8,(#*,(2X!!'B*!K[!242()2D)*!C#$%(*C!2,*!)(C#*%!** **(0!'2D)*!ME!2)-09!5(#B!#B*(,!,*I-,#*%!*0%I-(0#C!**

**20%!2,*!C$..2,(`*%!D,(*6)&!B*,*X!**

**=0!2!9,**0B-$C*!*/I*,(.*0#E!+-$#(0!*#!2)X!VMNNZW!#*** **C#*%!KO!0-0^8,-I!CI*8(*C!#-!(04*C#(92#*!#B*!**

**C*0C(#(4(#&!-6!%(66*,*0#!CI*8(*C!#-![!B*,D(8(%*CE!(** **08)$%(09!D,-.-/&0()^-8#20-2#*!V2C!"2/-** **r** 

# E!MNo!

**D,-.-/&0()^-8#20-2#*WX!!'B*!CI*8(*C!C*)*8#*%!6-,!#B** ***!*/I*,(.*0#!(08)$%*%!6(4*!CI*8(*C!6,-.!#B*!**

**>C#*,28*2*!62.()&!V%2(C(*CWE!6-$,!6,-.!#B*!12.(28*2** ***!62.()&!V.(0#WE!#5-!6,-.!#B*!G-)&9-028*2*!**

**62.()&!VD$8T5B*2#W!20%!6-$,!2%%(#(-02)!CI*8(*C!V"82** **,)*#!I(.I*,0*)!** Anagallis arvensis **!1XE!** Bayer CropScience Report Number US 0202

Page 11 of 85

# 6-/9)-4*! Digitalis purpurea **!1XE!5()%!.$C#2,%!** Sinapis arvensis **!1XE!20%!I-II&!** Papaver rhoeas

**1XWX!!>6#*,!8-0%$8#(09!2!,209*^6(0%(09!#*C#E!D,-.-/** **&0()!52C!2II)(*%!#-!C**%)(09C!2#!6-$,!,2#*C!**

**,209(09!6,-.!ZN!#-!MZN!9\B2!(0!2!C(09)*!#,*2#.*0#E!** **20%!IB&#-#-/(8(#&!52C!.*2C$,*%!2C!#B*!7H** **ON** 

# 6-,!9,-5#B!V%,&!5*(9B#W!26#*,!L!5**TCX!!7H **ON** 

# !42)$*C!,209*%!6,-.!SXP[!9!28#(4*!(09,*%(*0#!

**V2X(XW\B2!6-,!** Inula helenium **!1X!V>C#*,28*2*W!#-!PPXSZ!9!2X(X\B2!6-,!#B*!.(0#E!** Mentha spicata

**V12.(28*2*WX!!!**

**>0-#B*,!9,**0B-$C*!*/I*,(.*0#!52C!8-0%$8#*%!#-!(04*** **C#(92#*!#B*!#-)*,208*!-6!C#,25D*,,(*C!**

**V** Fragaria ananassa **!84X!** Cambridge Favourite **W!#-!D,-.-/&0()!20%!-#B*,!B*,D(8(%*CX!!H)2&!VKRPMW!**

**2II)(*%!2!MOo!7H!-8#20-2#*!*C#*,!6-,.$)2#(-0!-6!D,-** **.-/&0()!2#!MON!20%!KENNN!9\B2!#-!**

**C#,25D*,,(*C!2#!#B*!S^!#-!KM^)*26!C#29*X!!A*8,-C(C!-6!*/I-C*%!)*24*C!%*4*)-I*%!5** **(#B(0!2!6*5!%2&C!**

**-6!*/I-C$,*E!20%!.20&!)*24*C!5*,*!8-.I)*#*)&!0*8,-#(8!26#*,!M!5**TCX!!'B*** **!%,&!5*(9B#!-6!I)20#C!**

**52C!2)C-!C(90(6(820#)&!,*%$8*%!8-.I2,*%!#-!8-0#,-)!** **V2II,-/(.2#*)&!ZNo!,*%$8#(-0W!2#!D-#B!**

**2II)(82#(-0!,2#*Cd!#B*,*6-,*E!MON!9\B2!820!D*!(0#*,** **I,*#*%!2C!#B*!1]>71!6,-.!#B(C!C#$%&X!!'B*!**

**C#$%&!8-08)$%*%!#B2#!D,-.-/&0()!(C!(02II,-I,(2#*!6-** **,!5**%!8-0#,-)!(0!C#,25D*,,&!8,-ICX!**

**'B*!*66*8#C!-6!D,-.-/&0()!C-()!#,*2#.*0#C!-0!0-0^#2** **,9*#!8,-I!CI*8(*C!** Raphanus sativus **!V,2%(CBW!**

**20%!** Hordeum vulgare **!VD2,)*&W!5*,*!%*#*,.(0*%!(0!2!C*,(*C!-6!9,**0B-$C*** **!*/I-C$,*CX!!'B*!#*C#!**

**CI*8(*C!5*,*!I)20#*%!(0!.(/*%!C-()^C20%!.*%(2!8-0#2(0(09!8-08*0#,2#(-0C!-6!NXNK!#-!KNN!.9!**

**D,-.-/&0()\T9!C-()X!!<-))-5(09!2!Z^5**T!*/I-C$,*E!#** **B*!A]>7H!52C!%*#*,.(0*%!#-!D*!K!.9\T9!**

**6-,!D-#B!CI*8(*C!V?2,#*0!KRRNWX!!=0!2!)2D-,2#-,&!*/** **I*,(.*0#!%*C(90*%!#-!#*C#!#B*!#-)*,208*!-6!**

**5B*2#!V** T. aestivum **!1X!84X!A$92(0*CW!#-!D,-.-/&0()!-8#20-2#*E!"8B26*,!** **20%!HB()8#-*!VKRPNW!**

**,*I-,#*%!20!7:** **ON** 

# !6-,!,*%$8*%!9,-5#B!VD2C*%!-0!,*%$8*%!%,&!5*(9B#W!- **6!MEZNN!9!2X(X\B2E!2II)(*%!(0!**

**20!28*#-0*!C-)$#(-0X!!'B*!2$#B-,C!2)C-!#*C#*%!8-2C#** **!6(%%)*0*8T!V**

Amsinckia intermedia **!<(CBX!h!**

**Y*&XWE!2!C$C8*I#(D)*!#2,9*#!5**%!CI*8(*CE!20%!6-$0%** **!2!C(90(6(820#)&!)-5*,!7:** **ON** 

# !-6!MM!9\B2!6-,!

**8-2C#!6(%%)*0*8TX!**

**=0!2%%(#(-0!#-!9,**0B-$C*!20%!)2D-,2#-,&!*/I*,(.*0#** **CE!2!0$.D*,!-6!6(*)%!C#$%(*C!B24*!D**0!**

**8-0%$8#*%!#-!%*#*,.(0*!#B*!#-/(8(#&!-6!D,-.-/&0()!2** **II)(82#(-0C!-,!CI,2&!%,(6#!#-!8,-I!-,!0-0^**

**#2,9*#!I)20#C!CI*8(*CX!!>)^FB2#(D!*#!2)X!VKRRM2W!*4** **2)$2#*%!#B*!*66*8#C!-6!D,-.-/&0()!V2C!I*,8*0#!**

**4(C(D)*!(0m$,&!20%!,*%$8*%!B*(9B#W!-0!0-0^#2,9*#!K^!20%!M^&*2,^-)%!8B*,,&!#,**C!V** Prunus avium **W!**

**(0!6(*)%!#*C#!I)-#C!$C(09!6-$,!2II)(82#(-0!,2#*CX!!** **'B(,#&!%2&C!26#*,!#,*2#.*0#!V:>'WE!I)20#!(0m$,&!**

**52C!)-5!VN**

! **KMoE!1]>71s!KZXM!9!2X(X\B2WE!20%!I)20#!B*(9B#!52C!$** **0266*8#*%!8-.I2,*%!#-!**

**$0#,*2#*%!8-0#,-)CX!!+,-.-/&0()!82$C*%!.-%*,2#*!8B)** **-,-#(8!CI-#C!20%!)*26!8,(0T)(09E!D$#!0-,.2)!**

**0*5!9,-5#B!8-0#(0$*%!20%!I)20#C!,*8-4*,*%X!!G)20#C!** **,*8-4*,*%!26#*,!KMN!%2&C!VA]>71!s!KZM!**

**92X(X\B2WX!!'5-^&*2,^-)%!#,**C!5*,*!.-,*!,*C(C#20#!** **V1]>71!LN!:>'!s!KZM!9!2X(X\B2WX!!**

**+,-.-/&0()!%(%!0-#!266*8#!8B*,,&!#,**!B*(9B#!2#!20&** **!2II)(82#(-0!,2#*!#*C#*%!VA]>71!s!KZM!9!**

**2X(X\B2WX!!!**

**=0!20-#B*,!6(*)%!C#$%&!$C(09!#B*!C2.*!2II)(82#(-0!,** **2#*CE!>)^FB2#(D!*#!2)X!VKRRMDW!2CC*CC*%!#B*!**

**IB&#-#-/(8(#&!-6!D,-.-/&0()!#-!2)62)62!V** Medicago sativa **W!6,-.!I-#*0#(2)!B*,D(8(%*!%,(6#X!!'B(C!**

**C#$%&!CB-5*%!0-!I)20#!(0m$,&!-,!*66*8#C!-0!2)62)62!** **I-I$)2#(-0E!B*(9B#E!-,!&(*)%!2#!#B*!B(9B*C#!**

**2II)(82#(-0!,2#*!VA]>71!s!KZM!9!2X(X\B2WX!!=0!2!C(.** **()2,!C#$%&!$C(09!5(0*!9,2I*C!V** Vitis vinifera

**1XWE!>)^FB2#(D!*#!2)X!VKRRLW!8-0%$8#*%!#B,**!2II)(8** **2#(-0C!-4*,!2!M^&*2,!I*,(-%!20%!2CC*CC*%!I)20#!**

**(0m$,&!20%!820*!9,-5#B!6,-.!KZ!#-!KMN!:>'X!!q(C(D)*** **!(0m$,&!52C!,*I-,#*%!VN**

! **OPoWE!2)#B-$9B!**

***C#2D)(CB*%!I)20#C!,*8-4*,*%X!!<-,!D,-.-/&0()!2II)(** ***%!-0!0*5)&!I)20#*%!9,2I*CE!1]>71C!**

**VKZ!:>'W!,209*%!6,-.!ZXL!#-!KZXM!9!2X(X\B2E!2)#B-$9** **B!I)20#C!#,*2#*%!2#!#B*C*!,2#*C!,*8-4*,*%!RN!** Bayer CropScience Report Number US 0202

Page 12 of 85

# :>'X!!q(0*C!28B(*4*%![P

! **KKNo!820*!5*(9B#!(0!0*5)&!I)20#*%!9,2I*C!8-.I2,*%!#-!8-0#,-)!**

**V1]>71!s!ZMX[!9!2X(\B2WX!!_-5*4*,E!#B*,*!52C!0-!*66*8#!-0!#B*!820*!5*(9B#!(0!#,*2#*%!**

***C#2D)(CB*%!9,2I*CX!!'B*C*!C#$%(*C!(0%(82#*!#B2#!D,** **-.-/&0()!.2&!82$C*!(0m$,&!#-!0-0^#2,9*#!I)20#C!**

**2#!2II)(82#(-0!,2#*C!#B2#!2,*!5(#B(0!#B*!,209*!-6!,** ***8-..*0%*%!$C*CX!**

**3*8-..*0%*%!2II)(82#(-0!,2#*C!2,*!2)C-!5(#B(0!#B*!,** **209*!-DC*,4*%!D&!J29C8B(#`!VKRPRW!6-,!**

**8-0#,-)!-6!#B*!5**%!0$#C*%9*!V** Cyperus esculentus **!1XW!(0!#$,69,2CCX!!'B*!A]>71!6-,!0$#C*%9*!**

**8-0#,-)!V2C!C$,4(42)E!D2C*%!-0!0$.D*,!-6!I)20#C!I*,** **!$0(#!2,*2W!52C!NXMO!)D!2X(X\B2E!20%!2#!#B(C!**

**C2.*!,2#*!#$,69,2CC!52C!2)C-!0-#!(0m$,*%X!!]n"$))(4** **20!20%!]n:-0-420!VKRSMW!-DC*,4*%!B(9B*,!**

**D,-.-/&0()!0-^*66*8#!2II)(82#(-0!,2#*C!6-,!4-)$0#**** **,!5B*2#!V** Triticum aestivum **!1XWE!D2,)*&!**

**V** Hordeum vulgare **!1XWE!20%!-2#C!V** Avena sativa **!1XW!(0!62,.!6(*)%CX!!>II)(82#(-0C!$I!#-!LON!9!**

**2X(X\B2!%(%!0-#!8-0#,-)!#B*C*!CI*8(*C!VA]>71WE!2)#B-$9B!SL**

! **KNNo!#-I9,-5#B!T())!52C!-DC*,4*%!**

**(0!,2I*C**%!V**

Brassica campestris **!1XW!M**

! **MS!:>'!2#!#B(C!C2.*!,2#*X!!=0!20-#B*,!*/I*,(.*0#!-0**

**5**%!8-0#,-)!D&!G2,-8B*##(!VKRPLWE!KZN!9!2X(X\B2!8-** **.I)*#*)&!8-0#,-))*%!m(.C-05**%!V** Datura

stromonium **1XWE!D$#!%(%!0-#!2%4*,C*)&!266*8#!#B*!8,-I!CI*8(*CE** **!C-&D*20!V** Glycine max **!84X!F*0#WX!!**

**H-0C(C#*0#!5(#B!#B*!,*C$)#C!-6!#B(C!C#$%&E!Q2/!*#!2** **)X!VKRPZW!8-0%$8#*%!2!8-.D(0*%!6(*)%!20%!**

**9,**0B-$C*!C#$%&!-0!C*4*,2)!C-&D*20!8$)#(42,CX!!GB&** **#-#-/(8(#&E!2C!;$20#(6(*%!D&!4(C(D)*!(0m$,&!**

**2CC*CC.*0#!20%!,*%$8*%!&(*)%E!-88$,,*%!6-))-5(09!2I** **I)(82#(-0C!-6!LNN!9!#*8B0(82)!**

**D,-.-/&0()\B2E!5B()*!#B*!1]>71!6-,!4(C(D)*!(0m$,&!#** **-!C-&D*20C!52C!KNN!9!2X(X\B2X!!_-5*4*,E!**

**I)20#C!;$(8T)&!,*8-4*,*%!6,-.!#B(C!)-5*,!*/I-C$,*X!!"20%,2)!*#!2)X!VKRR** **PW!2)C-!8-0%$8#*%!2!**

**9,**0B-$C*!*/I*,(.*0#!%*#*,.(0(09!#B*!#-/(8(#&!-6!#** **B*!D,-.-/&0()!** n **^-8#20-&)!*C#*,!#-!**

**C$D#*,,20*20!8)-4*,!V** Trifolium subterraneum **!1X!844X!',(TT2)2WE!20%!,*I-,#*%!,*%$8*%!B*,D29*!**

**&(*)%!2#!.$8B!)-5*,!2II)(82#(-0!,2#*CX!!'B(C!C#$%&!** **,*I-,#*%!7H** **ON** 

# C!,209(09!6,-.!NXKS!#-!NXLZ!9!

**2X(X\B2!E!%*I*0%(09!-0!CI,2&(09!#(.*E!)*26!C#29*E!2** **0%!8$)#(42,!#*C#*%X!**

**Y2,#(0!*#!2)X!VKRRNW!-DC*,4*%!,*C(C#208*!(0!5B*2#!V** T. aestivum **!1XW!#,*2#*%!5(#B!OON!9!2X(X\B2!**

**D,-.-/&0()!(0!2!8-.D(0*%!6(*)%!20%!)2D-,2#-,&!C#$%&** **!5(#B!0-!*66*8#!-0!9,-5#BE!&(*)%E!-,!I,-#*(0!**

**8-0#*0#!-6!9,2(0d!20%!G20#-0*!20%!+2T*,!VKRRMW!CB-5** ***%!#B2#!#B,**!,(8*!8$)#(42,C!V** Oryza sativa **!1X!**

**844X!1*.-0#E!Y2,CE!'*D-00*#W!5*,*!C(.()2,)&!#-)*,20** **#!VA]>71!s!LNN!92X(X\B2WX!!'B*!#-/(8(#&!-6!**

**20!$0CI*8(6(*%!D,-.-/&0()!*C#*,!20%!D,-.-/&0()!IB*0** **-)!#-!.2(`*!V** Zea mays **W!C**%)(09C!V[^P!)*26!**

**C#29*W!52C!2)C-!%*#*,.(0*%!V<-$82,#!*#!2)X!KRSSWX!!** **]0!%2&C!-0*!20%!C(/!-6!#B*!*/I*,(.*0#E!**

**2II)(82#(-0C!-6!LLS!9!2X(\B2!-,!ONN!9!2X(X\B2E!,*CI** ***8#(4*)&E!5*,*!2II)(*%!#-!6(*)%^I)20#*%!**

**C**%)(09CX!!+-#B!2II)(82#(-0!,2#*C!8-0C#(#$#*%!$0D-$0%*%!A]>71!42)$*CE!C(08*!0-!2%4*,C*!**

***66*8#C!5*,*!-DC*,4*%!(0!*/I-C*%!.2(`*!C**%)(09CE!2** **)#B-$9B!#B*!#2,9*#!"-)2028*2*!0(9B#CB2%*!**

**5**%!52C!8-0#,-))*%X!!_-5*4*,E!2C!#B*!D,-.-/&0()!*C** **#*,!52C!$0CI*8(6(*%!V*X9XE!2C!D$#&,2#*E!**

**B*I#20-2#*E!-,!-8#20-2#*WE!#B*!,*)*4208&!-6!#B(C!*0** **%I-(0#!8200-#!D*!%*#*,.(0*%X!!<(02))&E!Q()C-0!**

**20%!+$,0C(%*!VKRPLW!8-0%$8#*%!9,**0B-$C*!20%!6(*)%!*/I*,(.*0#C!#-!%*#*,.(0*!#B*!,*)2#(4*!**

**(.I28#C!-6!D,-.-/&0()!2II)(82#(-0C!-0!C-&D*20C!20%!2CC-8(2#*%!5**%!CI*8(*CX!!>!C(09)*!**

**2II)(82#(-0!-6![NN!9!2X(X\B2!52C!%*#*,.(0*%!#-!2%4*** **,C*)&!(.I28#!5**%!CI*8(*Cn!9,-5#BE!D$#!%(%!**

**0-#!B24*!20&!*66*8#!-0!C-&D*20CX!!"$DC*;$*0#)&E!20!** **$0D-$0%*%!A]>71!-6![NN!9!2X(X\B2!820!D*!**

**%*#*,.(0*%!6-,!C-&D*20C!*/I-C*%!#-!D,-.-/&0()!V9,**** **0B-$C*!,*C$)#C!5*,*!m$%9*%!$0288*I#2D)*!**

**D*82$C*!#B*!C-)*!2II)(82#(-0!,2#*!#*C#*%E!KKNN!9!2X** **(X\B2E!*)(8(#*%!*66*8#C!(0!2))!*/I-C*%!CI*8(*CWX!!** Bayer CropScience Report Number US 0202

Page 13 of 85

# Unacceptable Studies



# U0%*,!]GG!8,(#*,(2E!2!#-#2)!-6!KO!I2I*,C!5*,*!%*#*, **.(0*%!#-!D*!$0288*I#2D)*!D*82$C*!#B*&!62()*%!**

**#-!,*I-,#!2II,-I,(2#*!*0%I-(0#CE!$C*%!(.I,-I*,!8-0#** **,-)CE!20%\-,!2II)(*%!D,-.-/&0()!2C!I2,#!-6!20!**

**B*,D(8(%*!.(/#$,*X!!'B*C*!I2I*,C!2,*!,*4(*5*%!D,(*6** **)&!B*,*X!**

**7C#*,!20%!A(mt0C#*(0!VKRR[W!8-2#*%!C**%C!5(#B!#B*!6** **-,.$)2#(-0!+,-.-/&0()!MON!"H!#-!9(4*!O!9!**

**2X(X\T9!C**%Cd!#B(C!*/I-C$,*!,*C$)#*%!(0!2![L**

! **[Po!,*%$8#(-0!(0!I*,*00(2)!,&*9,2CC!9*,.(02#(-0X!!**

**_-5*4*,E!C(08*!-0)&!-0*!8-08*0#,2#(-0!52C!#*C#*%E!0** **-!*0%I-(0#!8-$)%!D*!%*#*,.(0*%!6,-.!#B*!**

**242()2D)*!%2#2X!!"(.()2,)&E!2II)(82#(-0!-6!NXO!)DC!** **I*,!28,*!-6!20!$0CI*8(6(*%!D,-.-/&0()!**

**6-,.$)2#(-0!#-!6(*)%^I)20#*%!5B*2#!C**%)(09C!82$C*%** **!2!C(90(6(820#!,*%$8#(-0!(0!9,2(0!&(*)%!5B*0!**

**2II)(*%!#-!C#29*!K!C**%)(09CE!20%!C)(9B#!IB&#-#-/(8(#&!5B*0!2II)(*%!#-!C#29*!M!20%** **!Z!C**%)(09C!**

**V=C-.!20%!Q*09!KRPPWX!!_-5*4*,E!2!82)8$)2#*%!*0%I-(** **0#!52C!0-#!,*I-,#*%X!!>CB#-0!*#!2)X!VKR[SW!**

**,*I-,#*%!2!LMo!,*%$8#(-0!(0!I,-#*-)&#(8!28#(4(#&!6-** **,!** Cucurbita maxima **!8$8$.D*,C!9,-50!6,-.!**

**C**%C!C-2T*%!(0!2!KN**

! **Z** 

# !Y!D,-.-/&0()!C-)$#(-0d!B-5*4*,E!2!82)8$)2#*%!*0%I- **(0#!8-$)%!0-#!D*!**

**(0#*,I,*#*%E!2C!-0)&!-0*!8-08*0#,2#(-0!52C!#*C#*%X!** **!'B,**!D,-.-/&0()!2II)(82#(-0C!-6!LNN!9!**

**2X(X\B2!-4*,!K!&*2,!82$C*%!C(90(6(820#!(0m$,&!20%!,** ***%$8*%!C$,4(42)!20%!&(*)%!(0!*/I-C*%!** Trifolium

**CIX!I)20#C!V=420&!*#!2)X!MNNNWE!D$#!D*82$C*!-0)&!-0*!2II)(82#(-0!,2#*!52C!#*C#*%E!#B*,*!52C!0-!**

**D-$0%(09!-6!*/I-C$,*C!20%!*66*8#CX!!"(.()2,)&E!:*2,** **!20%!"20%,2)!VKRRRW!2II)(*%!D,-.-/&0()!** n

**^** **-8#20-&)!*C#*,!VMNN!9!2X(X\1!2#!KXZ!1\B2W!(0!#5-!C$** **88*CC(4*!&*2,C!#-!#B*!#5-!8)-4*,!8$)#(42,C!20%!**

**)$8*,0*!(0!2!6(*)%!I)-#!*/I*,(.*0#X!!7/I-C$,*!!82$C** ***%!MP**

! **LKo!)*26!D$,0!(0!#B*!8)-4*,!6-,!#B*!6(,C#!**

**&*2,E!D$#!#B*,*!52C!0-!C(90(6(820#!%(66*,*08*!#B*!C** ***8-0%!&*2,X!!'B*,*6-,*E!MSN!9!2X(X\B2!52C!**

**(0#*,I,*#*%!2C!#B*!$0D-$0%*%!1]>71E!D$#!#B*!C#$%&!,** ***.2(0C!$0288*I#2D)*!D*82$C*!-6!-0)&!2!**

**C(09)*!#,*2#.*0#!,2#*X!!>!C(09)*!2II)(82#(-0!-6!MSN** **!9!2X(X\B2!82$C*%!KNNo!.-,#2)(#&!-6!(,-05**%!**

**V** Vernonia galamensis **W!C**%)(09C!(0!D-#B!9,**0B-$C*!20%!6(*)%!*/I*,(.*0#** **C!V3-C*D*,9!KRRPWX!!**

**J$%&!*#!2)X!VKRRNW!,*I-,#*%!#B2#!*/I-C$,*!#-!NXKo!+** **$8#,()!2)#*,*%!8B)-,-IB&))!6)$-,*C8*08*!(0!**

**C-&D*20!20%!D2,)*&!C**%)(09CE!D$#!-0)&!#*C#*%!-0*!8** **-08*0#,2#(-0!20%!,*I-,#*%!0-!-#B*,!*0%I-(0#CX!!**

**A-!*66*8#!-6!#B*!6-,.$)2#(-0!+,-.(02)!7H!-0!)$8*,0*** **!V** Medicago falcate **W!0-%$)*!6-,.2#(-0!-,!**

**D(-.2CC!52C!-DC*,4*%!26#*,![!5**TC!-6!9,-5#B!(0!CI(** **T*%!C-()X!!_-5*4*,E!#B*!2$#B-,C!#*C#*%!-0)&!**

**-0*!8-08*0#,2#(-0!VMXO!1!+,-.(02)\B2W!20%!62()*%!#-** **!,*I-,#!#B*!2.-$0#!-6!28#(4*!(09,*%(*0#!(0!**

**#B(C!#,*2#.*0#!Vq20!3*0CD$,9!20%!"#,(m%-.!KRSZWX!**

**F,2#T&!20%!Q2,,*0!VKRPKW!%*#*,.(0*%!#B*!#-/(8(#&!-6** **!#*8B0(82)^9,2%*!D,-.-/&0()!#-!9*,.(02#(09!**

**C-,9B$. V**  Sorghum vulgare **WE!-2#C!V** Avena sativa **WE!20%!8$8$.D*,!V** Cucumis sativus **WX!!'5-!**

**8-08*0#,2#(-0C!5*,*!#*C#*%E!K!20%!KN!II.E!20%!,--#!** **20%!CB--#!9,-5#B!5*,*!%*#*,.(0*%X!!QB()*!**

**9,-5#B!(0B(D(#(-0!52C!)*CC!#B20!ONo!6-,!2))!CI*8(*C** **!#*C#*%!2#!K!II.E!(#!52C!9,*2#*,!#B20!ONo!6-,!**

**-2#C!*/I-C*%!#-!KN!II.!D,-.-/&0()X!!_-5*4*,E!#B*!2$** **#B-,C!%(%!0-#!,*I-,#!28#$2)!,2#*C!-6!9,-5#B!**

**(0B(D(#(-0E!D$#!C(.I)&!5B*#B*,!*/I-C$,*!,*C$)#*%!(0** **!)*CC!#B20!-,!9,*2#*,!#B20!ONo!(0B(D(#(-0!-6!**

**,--#!-,!CB--#!9,-5#BX!!'B*,*6-,*E!#B*!I2I*,!(C!$028** **8*I#2D)*!$0%*,!]GG!8,(#*,(2X!**

**>!C*,(*C!-6!5(0%^#$00*)!C#$%(*C!52C!8-0%$8#*%!#-!%*** **#*,.(0*!#B*!*66*8#C!-6!2(,D-,0*!D,-.-/&0()^**

**-8#20-2#*!6-,.$)2#(-0!H*,#,-)!+!#-!0-0^#2,9*#!C$06)** **-5*,!V** Helianthus annuus **W C** %)(09C!V<-))2T!**

**20%!_$,)*!MNNL2EDE!MNNOWX!!=0!#B*!6(,C#!C#$%&!V<-))** **2T!20%!_$,)*!MNNL2WE!*66*8#C!-0!IB-#-C&0#B*C(C!**

**20%!9,-5#B!5*,*!;$20#(6(*%!4(2!8B)-,-IB&))!6)$-,*C8** ***08*!20%!I)20#!D(-.2CCX!!'B*!)-5*C#!**

**8-08*0#,2#(-0!#B2#!266*8#*%!;$20#$.!&(*)%!(0!0*5)&!%*4*)-I*%!)*24*C!52C!NXPS!p9\.** **L**



# V(0#*,I,*#*%!2C!#B*!1]>7HWX!!'B*!A]>7HC!6-,!I)20#!D **(-.2CC!5*,*!#B*!C2.*!6-,!#B*!M^)*26!20%!**

**Z^)*26!C#29*CE!2#!KXM!p9\.** **L** 

# X!!'B*!,*CI*8#(4*!8B)-,-IB&))!6)$-,*C8*08*!A]>7H!20 **%!7H ON**  

# C!6-,!

**C$06)-5*,!)*24*C!%*4*)-I*%!I-C#^*/I-C$,*!5*,*!,*I-,#*%!(0!2!C*8-0%!C#$%&!V<-))2T!20%!_$,)*!** Bayer CropScience Report Number US 0202

Page 14 of 85

# MNNLDW!2C!NXM[!p9\. **L** 

# !20%!KXZZ!p9\. **L** 

# !VM!:>'WX!!G)20#C!*/I-C*%!#-!2!.2/(.$.!-6!KXLK!p9\. **L**



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# E!,*CI*8#(4*)&E!6-,!)*24*C!%*4*)-I*%!26#*,!*/I-C$,* **X!!:*CI(#*!#B*C*!,*C$)#CE!**

**#B(C!C*,(*C!-6!I2I*,C!52C!m$%9*%!#-!D*!$0288*I#2D)*** **!$0%*,!]GG!8,(#*,(2E!C(08*!8-0#,-)!I)20#C!5*,*!**

**B*)%!$0%*,!%(66*,*0#!8-0%(#(-0C!V(X*XE!(0!2!9,**0B-** **$C*W!20%!5*,*!0-#!C$Dm*8#*%!#-!5(0%!#$00*)CX!**

**"(.()2,)&E!"`(9*#(!*#!2)X!VKRSKW!-DC*,4*%!#B2#!P^%2** **&!-)%!5B*2#!C**%)(09C!(..*,C*%!(0!2!LN!p.-)!**

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**8-0#,-)!I)20#C!5*,*!*/I-C*%!#-!2!I,-I*,!C-)4*0#!8-0** **#,-)!V*X9XE!(..*,C*%!(0!2!:Y"]^-0)&!**

**C-)$#(-0WX!!>C!C$8BE!#B(C!,*6*,*08*!52C!%**.*%!$028** **8*I#2D)*!$0%*,!]GG!8,(#*,(2X!!**

**Y2,CB2))!20%!+(,0(*!VKRSOW!(04*C#(92#*%!#B*!*66*8#C** **!-6!2!D,-.-/&0()u(-/&0()!*C#*,!7H!**

**2II)(82#(-0!-6!P[N!9!2X(X\B2!-0!K[!*%9*^-6^6(*)%!0-0^#2,9*#!I)20#C!CI*8(*CX!!]6!#B*!CI*8(*C!**

***/I-C*%E!-0)&!6-$,!V#B(C#)*** Carduus acanthoides **E!B*.)-8T** Conium maculatum **E!CI**%5*))**

Veronica persica **E!20%!.$C#2,%** Alliaria petiolata **W!5*,*!C*,(-$C)&!266*8#*%!D&!#B*!B*,D(8(%*X!!'B(C!**

**I2I*,!52C!m$%9*%!2C!$0288*I#2D)*!$0%*,!]GG!8,(#*,(2** **!D*82$C*!!0-!82)8$)2#*%!*0%I-(0#C!5*,*!**

**,*I-,#*%!20%!D,-.-/&0()!52C!#*C#*%!(0!8-08*,#!5(#B!** **20-#B*,!B*,D(8(%*!V(-/&0()WX!!_-5*4*,E!#B*!**

**2$#B-,C!8-08)$%*%!#B2#!#B(C!B*,D(8(%*!.(/#$,*!52C!-** **6!)(.(#*%!#-/(8(#&!#-!*%9*^-6^6(*)%!6)-,2d!**

**B*08*E!(#!820!D*!,*2C-02D)&!8-08)$%*%!#B2#!D,-.-/&0()!2)-0*!5-$)%!D*!$0)(T*)&!#-!2%4*,C*)&!**

**266*8#!C$8B!CI*8(*CX!!=0!2!C(.()2,!C#$%&E!2!C*,(*C!** **-6!I)20#C!5*,*!*/I-C*%!#-!20!B*,D(8(%*!.(/#$,*!**

**%*C(90*%!#-!2II,-/(.2#*!#B*!8-08*0#,2#(-0C!-6!B*,D(** **8(%*C!%*#*8#*%!(0!>)D*,#2E!H202%2!,2(062))!**

**V_())!*#!2)X!MNNMWX!!A-!C(90(6(820#!2%4*,C*!*66*8#C** **!5*,*!-DC*,4*%!(0!*/I-C*%!I-#2#-!C**%)(09CE!C-!**

**(#!820!D*!8-08)$%*%!#B2#!*/I-C$,*!#-!NXO!9!D,-.-/&0** **()\B2!2)-0*!5-$)%!B24*!0-!*66*8#!2C!5*))X!**

**=0!8-08)$C(-0E!0-0^#2,9*#!I)20#!*66*8#!)*4*)C!9*0*,2))&!,209*%!6,-.!2II)(82#(-0!,2#*** **C!(0!#B*!)-5!**

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**2%4*,C*)&!266*8#*%!D&!2II)(82#(-0C!-6!D,-.-/&0()!,2** **09(09!6,-.!MNN!#-![NN!9\B2X!!'B*!,*C$)#C!-6!**

**#B*!9,**0B-$C*!C#$%(*C!2,*!8-0C(C#*0#!5(#B!#B*C*!8-** **08)$C(-0C!20%!#B*!*66*8#!)*4*)C!6,-.!#B*!**

**9,**0B-$C*!C#$%(*C!2,*!5(#B(0!#B(C!1]>71!,209*X!!"#** **$%(*C!2)C-!C$99*C#!#B2#!5B()*!2(,D-,0*!**

**8-08*0#,2#(-0C!-6!D,-.-/&0()!.2&!82$C*!2%4*,C*!*66*** **8#C!(0!0-0^#2,9*#!CI*8(*CE!,*8-4*,&!820!**

**-88$,X!!=0!2%%(#(-0E!#B*!#-/(8(#(*C!-6!#*8B0(82)!D,** **-.-/&0()!20%!D,-.-/&0()!6-,.$)2#(-0C!2,*!**

**C(.()2,X**



# Invertebrates

**>!#-#2)!-6!*(9B#!I2I*,C!*/2.(0(09!#B*!#-/(8(#&!-6!D** **,-.-/&0()!#-!#*,,*C#,(2)!(04*,#*D,2#*C!5*,*!**

**(%*0#(6(*%!%$,(09!#B*!)(#*,2#$,*!C*2,8BE!-0)&!-0*!-6!5B(8B!52C!m$%9*%!#-!D*!288*I#2D)*!$0%*,!]GG!**

**8,(#*,(2X!**

**=9)*C(2C!*#!2)X!VMNNM2W!*42)$2#*%!#B*!#-/(8(#&!-6!#** **B*!6-,.$)2#(-0!+,-.-/&0()!MON!9\1!"H!#-!#B*!**

**C)$9!CI*8(*C!** Deroceras reticulatum **X!!")$9** ***99C!5*,*!(08$D2#*%!-0!2,#(6(8(2)!C-()!CI(T*%!5(#B!**

**+,-.-/&0()!!MON!9\1!"HE!20%!,*I-,#*%!Z^!20%!P^%2&!1** **: ON**  

# !42)$*C!5*,*!NXNN[!20%!NXNNL[R!.9!

**2X(X\8. M**  

# !V[NN!20%!L[R!9!2X(X\B2WE!,*CI*8#(4*)&X!!! Bayer CropScience Report Number US 0202

Page 15 of 85

# 'B*!,*.2(0(09!C*4*0!#*,,*C#,(2)!(04*,#*D,2#*!,*6*,* **08*C!62()*%!#-!.**#!]GG!288*I#2D()(#&!8,(#*,(2!**

**6-,!2!0$.D*,!-6!,*2C-0CE!(08)$%(09!62()$,*!#-!*/2.(** **0*!2II,-I,(2#*!*0%I-(0#CX!!Y-,#-0!20%!**

**Y-66*##!VKRPM2W!,*4(*5*%!#B*!*66*8#C!-6!B*,D(8(%*C!** **-0!** Apis mellifera **!B-0*&!D**CE!D$#!%(%!0-#!**

**I,*C*0#!-,(9(02)!%2#2X!!Y-,#-0!20%!Y-66*##!VKRPMDW!** ***/2.(0*%!#B*!%(*#2,&!#-/(8(#&!-6!#*8B0(82)!**

**D,-.-/&0()!VKNE!KNNE!KENNN!II.!(0!C$8,-C*!C&,$IW!#-** **!0*5)&!*.*,9*%!B-0*&D**CX!!_-5*4*,E!**

**#-/(8!*66*8#C!5*,*!*42)$2#*%!2C!#B*!B2)6^)(6*!-6!#B** ***!*/I-C*%!D**!I-I$)2#(-0!V*X9XE!#B*!0$.D*,!-6!**

**%2&C!D*#5**0!#*C#!(0(#(2#(-0!20%!#B*!%*2#B!-6!B2)6!** **-6!#B*!D**C!*/I-C*%!2#!*28B!%-C*!)*4*)WE!5B(8B!**

**52C!m$%9*%!#-!D*!20!(.I,-I*,!*0%I-(0#X!!=0#*,*C#(09** **)&E!#B*!)-5*,!#5-!%-C*C!C(90(6(820#)&!**

**(08,*2C*%!D**!)-09*4(#&!VLZXO!20%!LLXS!%2&C!2#!KN!2** **0%!KNN!II.!D,-.-/&0()E!,*CI*8#(4*)&W!2C!**

**8-.I2,*%!#-!8-0#,-)C!VKPXO!%2&CWE!2)#B-$9B!#B*!B2)6^)(6*!-6!#B*!D**!I-** **I$)2#(-0!6*%!2!%(*#!**

**8-0#2(0(09!KENNN!II.!D,-.-/&0()!52C!C(90(6(820#)&!,** ***%$8*%!VMXR!%2&CWX!!"2.Cv*^G*#*,C*0!**

**VKRROW!%*#*,.(0*%!#B*!#-/(8(#&!-6!#B*!6-,.$)2#(-0!D** **,-.-/&0()!V[NoW!u!H?>KKL[SPM!VMoW!#-!**

**#B*!,-4*!D**#)*!** Aleochara bilineata **X!!Y2#*%!2%$)#!6*.2)*C!5*,*!*/I-C*%!#-!2!D,-.-/&0()**

**6-,.$)2#(-0!8-08*0#,2#(-0!-6!2II,-/(.2#*)&!NX[!p1\8** **. M**  

# !V-,!SLN!II.W!6-,!KR!%2&CE!2)#B-$9B!#B(C!

***/I-C$,*!B2%!0-!C(90(6(820#!*66*8#!-0!.-,#2)(#&!-,!** ***99!I,-%$8#(-0X!!_-5*4*,E!(0!#B(C!82C*E!**

**D,-.-/&0()!52C!#*C#*%!2C!2!I2,#!-6!20!B*,D(8(%*!.(/** **#$,*E!20%!C-!8-$)%!0-#!D*!m$%9*%!2C!**

**288*I#2D)*!$0%*,!]GG!8,(#*,(2X!**

**'B*!,*.2(0(09!#B,**!$0288*I#2D)*!I2I*,C!;$20#(6(*%!** **#B*!,*%$8#(-0!(0!** D. reticulatum **!C)$9!6**%(09!**

**-0!8,-I!C**%)(09CE!5B(8B!(C!2)C-!8-0C(%*,*%!20!$028** **8*I#2D)*!*0%I-(0#!288-,%(09!#-!]GG!8,(#*,(2!**

**V7C#*,!20%!A(m*0C#*(0!KRROEKRR[d!A(m*0C#*(0!20%!7C#** ***,!KRRSWX!!=0!2%%(#(-0E!#B*!C)$9C!5*,*!**

***/I-C*%!#-!#B*!D,-.-/&0()!6-,.$)2#(-0C!V+,-.-/&0()!** **MMO!7H!20%!+,-.-/&0()!MON!"HW!#B,-$9B!**

**8,-I!C**%C!I,*^#,*2#*%!5(#B!#B*!B*,D(8(%*!2C!2!8-2#** **(09E!2!0-0^C#20%2,%!*/I-C$,*!.*#B-%X!!**

**+,-.-/&0()!C**%!#,*2#.*0#C!%(%!0-#!I,*4*0#!C)$9!6**%(09!-0!8,-I!C**%)(09CE!2)#B-$9B!#B*(,!**

**8-0C$.I#(-0!,2#*!52C!,*%$8*%!V7C#*,!20%!A(m*0C#*(0!** **KRROWX!!_-5*4*,E!2C!2!I2,#!-6!#B(C!**

***/I*,(.*0#E!C**%C!5*,*!2)C-!I,*^#,*2#*%!5(#B!20!$0(** **%*0#(6(*%!6$09(8(%*d!8-0C*;$*0#)&E!#B*C*!**

**C)$9C!5*,*!(0!628#!*/I-C*%!#-!2!D,-.-/&0()\6$09(8(%** ***!.(/#$,*X!!**

**<(02))&E!=9)*C(2C!*#!2)X!VMNNMDW!2)C-!#*C#*%!#B*!#-/(8(#&!-6!+,-.-/&0()!MON!9\1!"H!#-!** D.

reticulatum **!*99C!D&!(08$D2#(09!%*4*)-I(09!*99C!-0!I2I*,!C-2T*%** **!(0!2!B*,D(8(%*!C-)$#(-0X!!**

**_-5*4*,E!#B*!)-5*C#!#*C#*%!8-08*0#,2#(-0E** **NXNNLRL!.9!2X(X\8. M**  

# E!82$C*%!KNNo!.-,#2)(#&!26#*,!

**ZS!B-$,C!-6!*/I-C$,*X!!"$DC*;$*0#)&E!#B(C!42)$*!8-0** **C#(#$#*C!20!$0D-$0%*%!1H** **KNN** 

# E!20%!#B*,*6-,*!(C!

**0-#!2!,*)(2D)*!*0%I-(0#X**

**+2C*%!-0!#B*!,*C$)#C!I,*C*0#*%!B*,*E!D,-.-/&0()!2II** ***2,C!#-!D*!,*)2#(4*)&!0-0^#-/(8!#-!#*,,*C#,(2)!**

**(04*,#*D,2#*CX!!")$9!.-,#2)(#&!-88$,,*%!6-))-5(09!*** **/I-C$,*C!#B2#!2II,-/(.2#*!2II)(82#(-0!,2#*C!**

**-6!L[R!#-![NN!9!2X(\B2XE!5B()*!D**#)*C!5*,*!$0266*8** **#*%!D&!8-0#28#!5(#B!C$DC#,2#*!8-0#2(0(09!**

**SLN!II.!D,-.-/&0()X!!>)#B-$9B!m$%9*%!$0288*I#2D)*!$** **0%*,!]GG!8,(#*,(2E!,*C$)#C!6,-.!B-0*&D**!**

**C#$%(*C!(0%(82#*%!#B2#!8-0C$.I#(-0!-6!C$8,-C*!8-0#2** **(0(09!$I!#-!KNN!II.!D,-.-/&0()!(C!$0)(T*)&!**

**#-!82$C*!2%4*,C*!*66*8#C!-0!)-09*4(#&X!**



# Birds

**]0)&!#B,**!I$D)(82))&^242()2D)*!,*6*,*08*C!8-08*,0(** **09!#B*!#-/(8(#&!-6!D,-.-/&0()!#-!D(,%C!5*,*!**

**(%*0#(6(*%!#B,-$9B!#B*!2D-4*!C*2,8B*C!20%!0-0*!-6!#** **B*C*!.*#!]GG!288*I#2D()(#&!8,(#*,(2X!** Bayer CropScience Report Number US 0202

Page 16 of 85

# :$028B(*!20%!<)*#8B*,!VKRPNW!(0m*8#*%!B*0!V Gallus gallus domesticus **W!*99C!5(#B!KNE!MOE!ONE!20%!**

**KNN!II.!D,-.-/&0()!(0!28*#-0*!20%!,*I-,#*%!#B2#!#B*** **!)-5*C#!%-C*!,*C$)#*%!(0!2!B2#8B!,2#*!-6![Zo!**

**-6!8-0#,-)CE!5B()*!MO!II.!,*C$)#*%!(0!2![Ro!C$88*CC** **6$)!B2#8B!,2#*X!!:-C*C!-6!ON!II.!20%!9,*2#*,!**

**82$C*%!KNNo!*99!.-,#2)(#&X!!_-5*4*,E!*99!(0m*8#(-0!** **(C!2!0-0C#20%2,%!*/I-C$,*!,-$#*E!#B*!**

**2$#B-,C!%(%!0-#!,*I-,#!(6!8-0#,-)!*99C!5*,*!(0m*8#*** **%!5(#B!28*#-0*!#-!I,-4(%*!20!2II,-I,(2#*!**

**C-)4*0#!8-0#,-)E!20%!2!82)8$)2#*%!*0%I-(0#!52C!0-#!** **I,*C*0#*%!(0!#B*!I2I*,X!**

**'B*!#-/(8(#&!-6!#B*!D,-.-/&0()!6-,.$)2#(-0!+,-02#*** **r** 

# !VD,-.-/&0()!-8#20-2#*!u!YHG>W!#-!

**.2))2,%!%$8T!V**

Anas platyrhynchos **W!*99C!52C!%*#*,.(0*%!4(2!2!LN^C*8-0%!(..*,C(-0!(0!** **#B*!**

**B*,D(8(%*X!!'B*!2$#B-,C!,*I-,#*%!#B2#!#B*!1:** **ON!** 

# 52C!*;$(42)*0#!#-!6(*)%!2II)(82#(-0C!-6!PXO!)D\28,*!

**2#!KNN!92)\28,*!V_-66.20!20%!>)D*,C!KRSZWX!!_-5*4*,E!#B(C!52C!2!0-0C#20%2,%!*/I** **-C$,*!.*#B-%E!**

**#B*!#*C#*%!8-08*0#,2#(-0C!5*,*!0-#!,*I-,#*%!(0!#B*!** **I2I*,E!20%!#B*!#*C#*%!D,-.-/&0()!6-,.$)2#(-0!**

**2)C-!8-0#2(0*%!#B*!B*,D(8(%*!YHG>X!!H-0C*;$*0#)&E!#** **B*!C#$%&!%-*C!0-#!.**#!]GG!8,(#*,(2X!**

**<(02))&E!_())!20%!H2.2,%*C*!VKRS[W!#*C#*%!#B*!%(*#2** **,&!#-/(8(#&!-6!#B*!C2.*!6-,.$)2#(-0!V+,-02#*W!**

**#-!KZ^%2&^-)%!J2I20*C*!;$2()!V** Coturnix coturnix japonica **WX!!'B*&!%*#*,.(0*%!#B2#!#B*!O^%2&!**

**%(*#2,&!1:** **ON** 

# !-6!#B*!D,-.-/&0()^YHG>!.(/#$,*!52C!9,*2#*,!#B20!OE **NNN!II.E!2)#B-$9B!#B*&!%(%!**

**0-#*!,*%$8*%!8-0C$.I#(-0!-6!+,-02#*** **r** 

# ^8-0#2(0(09!6**%X!!>C!5(#B!#B*!I,*4(-$C!,*6*,*08*E!#B*C*!

**,*C$)#C!2,*!-6!)(.(#*%!42)$*!20%!62()!#-!.**#!]GG!8** **,(#*,(2!2C!D,-.-/&0()!52C!#*C#*%!2C!2!I2,#!-6!20!**

**B*,D(8(%*!.(/#$,*X!!_-5*4*,E!C(08*!#B*!D,-.-/&0()^Y** **HG>!B*,D(8(%*!.(/#$,*!52C!%*#*,.(0*%!#-!**

**D*!0-0^#-/(8!$0%*,!#B*C*!8-0%(#(-0CE!(#!(C!)(T*)&!#** **B2#!D,-.-/&0()!2)-0*!5-$)%!2)C-!D*!0-0^#-/(8!**

**#-!J2I20*C*!;$2()X!!**



# Mammals

**'B*!)(#*,2#$,*!C*2,8B!,*#$,0*%!-0*!I2I*,!#B2#!8-0C(** **%*,*%!#B*!*8-#-/(8(#&!-6!+,-.(02)** **r** 

# !VMSXOo!

**D,-.-/&0()!u!MSXOo!YHG>W!#-!6(*)%!I-I$)2#(-0C!-6!C.** **2))!.2..2)CE!(08)$%(09!.*2%-5!4-)*C!**

**V** Microtus pennsylvanicus **WE!m$.I(09!.(8*!V** Zapus hudsonius **WE!20%!B-$C*!.(8*!V** Peromyscus

leucopus **WX!!'B(C!I2I*,!52C!0-#!%**.*%!288*I#2D)*!$0%*,!]GG!** **8,(#*,(2!C(08*!0-!C#20%2,%!*0%I-(0#!**

**52C!82)8$)2#*%E!#B*!#*C#*%!6-,.$)2#(-0!8-0#2(0*%!YHG>E!20%!-0)&!-0*!8-08*0#,2#(-0!52C!#*C#*%!**

**(0!6(*)%!I)-#CX!!'B*!2$#B-,C!%(%!0-#*!#B2#!** M. pennsylvanicus **!4-)*C!*/I-C*%!#-!+,-.(02)** **r**



# */B(D(#*%!(08,*2C*%!(08(%*08*C!-6!-I2;$*!8-,0*2C!VH **)2,T!*#!2)X!KRR[WX!!_-5*4*,E!(#!8200-#!D*!**

**%*#*,.(0*%!(6!#B(C!*66*8#!5-$)%!D*!-DC*,4*%!(0!4-)*** **C!*/I-C*%!C-)*)&!#-!D,-.-/&0()X!**



# References

**>)^FB2#(D!FE!G2,T*,!3E!<$*,C#!7GX!"5**#!8B*,,&!V** Prunus avium **W!,*CI-0C*!#-!C(.$)2#*%!%,(6#!**

**6,-.!C*)*8#*%!B*,D(8(%*CX!Q**%!'*8BX!KRRM2X![VZW@RP** **O**

! **RPRX!**

**>)^FB2#(D!FE!G2,T*,!3E!<$*,C#!7GX!>)62)62!V** Medicago sativa **W!,*CI-0C*!#-!C(.$)2#*%!B*,D(8(%*!**

**CI,2&!%,(6#X!Q**%!'*8BX!KRRMDX![VZW@RO[**

! **R[NX!**

**>)^FB2#(D!FE!G2,T*,!3E!<$*,C#!7GX!KRRLX!Q(0*!9,2I*!** **V** Vitis vinifera **!1XW!,*CI-0C*!#-!C(.$)2#*%!**

**B*,D(8(%*!%,(6#XKRRLX!PVKW@RP**

! **KNMX!** Bayer CropScience Report Number US 0202

Page 17 of 85

# >CB#-0!<YE!G*00*,!:E!_-66.20!"X!766*8#!-6!C*4*,2)!B ***,D(8(%*C!-0!I,-#*-)&#(8!28#(4(#&!-6!C;$2CB!**

**C**%)(09CX!Q**%!"8(X!KR[SX!K[VMW@K[R**

! **KPKX!**

**+-$#(0!HE!7).*922,%E!AE!Fm2*,!HX!'-/(8(#&!#*C#(09!-** **6!6(6#**0!0-0^8,-I!I)20#!CI*8(*C!5(#B!C(/!**

**B*,D(8(%*C!(0!2!9,**0B-$C*!*/I*,(*.#0@!(.I)(82#(-0C** **!6-,!,(CT!2CC*CC.*0#X!78-#-/(8-)X!MNNZX!**

**KL@LZR**

! **L[RX!**

**+,--T*!1'E!H2))!:JE!?*(9*,!:1E!A-,#B8$##!H7d!*%CX!>8$#*!#-/(8(#&!-6!-,920(8!8B*.(82)C!#-!**

**62#B*2%!.(00-5C!V**

Pimephales promelas **WX!q-)!=X!KRSZX!H*0#*,!6-,!12T*!"$I*,(-,!704(,-0.*0#2)!**

**"#$%(*CE!U0(4*,C(#&!-6!Q(C8-0C(0^"$I*,(-,X!**

**+$B)!FJE!<2*,D*,!A1X!>8$#*!#-/(8(#&!-6!C*)*8#*%!B*,** **D(8(%*C!20%!C$,628#20#C!#-!)2,42*!-6!#B*!**

**.(%9*!** Chironomus riparius **X!>,8BX!704(,-0X!H-0#2.X!'-/(8-)X!KRSRX!KS@OLN**

! **OL[X!**

**+$B)!FJE!_2.()#-0!"JE!"8B.$)D28B!JHX!HB,-0(8!#-/(8(** **#&!-6!#B*!D,-.-/&0()!6-,.$)2#(-0!+$8#,()!**

**#-!** Daphnia magna ***/I-C*%!8-0#(0$-$C)&!20%!(0#*,.(##*0#)&X!>,8BX!704(** **,-0X!H-0#2.X!'-/(8-)X!**

**KRRLX!MO@KOM**

! **KORX!**

**H2(0!JX3XE!H2(0!3FX!'B*!*66*8#C!-6!C*)*8#*%!B*,D(8(** **%*C!-0!`&9-CI-,*!9*,.(02#(-0!20%!9,-5#B!-6!**

Chlamydomonas moewusii **!VHB)-,-IB&8*2*E!q-)4-82)*CWX!JX!GB&C(-)X!KRSLX!KR@** **LNK**

! **LNOX!**

**H)2,T!:3E!Y-$)#-0!H>E!_(0*C!J7E!_-66.20!:JX!".2))!.** **2..2)!I-I$)2#(-0C!(0!Y2,&)20%!**

**.*2%-5C!%$,(09!6-$,!&*2,C!-6!B*,D(8(%*!V+,-.(02)rW!** **>II)(82#(-0CX!704(,-0X!'-/(8-)X!HB*.X!**

**KRR[X!KOVRW@KOZZ**

! **KOONX!!**

**H)2&!:qX!'B*!,*CI-0C*!-6!C#,25D*,,&!#-!2!,209*!-6!6** **-)(29*!28#(-0!B*,D(8(%*X!G,-8X!KK** **#B** 

# !+,X!Q**%!

**H-0#,-)!H-06X!KRPMX!**

**H$))(.-,*!:X3X!'B*!** in vitro **C*0C(#(4(#&!-6!C-.*!CI*8(*C!-6!HB)-,-IB&8*2*!#-!2!C** ***)*8#*%!,209*!-6!**

**B*,D(8(%*CX!Q**%!3*C*2,8BX!KRPOX!KO@ZNK**

! **ZN[X!**

**:*2,!+"E!"20%,2)!?>X!'B*!IB&#-#-/(8(#&!-6!#B*!B*,D(** **8(%*C!D,-.-/&0()E!I&,(%2#*E!(.2`*#B2I&,!**

**20%!2!D,-.-/&0()!u!%(6)$6*0(820!.(/#$,*!-0!C$D#*,,2** **0*20!8)-4*,!20%!)$8*,0*!C**%)(09CX!>$C#,2)X!**

**JX!7/IX!>9,(X!KRRRX!LR@SLR**

! **SZPX!**

**:$028B(*!J<E!<)*#8B*,!QQX!'B*!#-/(8(#&!-6!8*,#2(0!B** ***,D(8(%*C!#-!B*0Cn!*99C!2CC*CC*%!D&!#B*!*99^**

**(0m*8#(-0!#*8B0(;$*X!>00X!>II)X!+(-)X!KRPNX![[@OKO**

! **OMNX!**

**7C#*,!>E!A(mt0C#*(0!J_X!H-0#,-)!-6!#B*!6(*)%!C)$9!** Deroceras reticulatum **VYw))*,W!VG$).-02#2@!**

**1(.28(%2*W!D&!I*C#(8(%*C!2II)(*%!#-!5(0#*,!5B*2#!C*** ***%X!H,-I!G,-#X!KRROX!KZVOW@ZNR**

! **ZKLX!!**

**7C#*,!>E!A(mt0C#*(0!J_X!H-0#,-)!-6!6(*)%!C)$9!V** Deroceras reticulatum **!VYw))*,WW!D&!C**%^2II)(*%!**

**I*C#(8(%*C!(0!I*,*00(2)!,&*9,2CC!2CC*CC*%!D&!)2D-,2** **#-,&!#*C#CX!g!G6)20`*0T,!G6)20`*0C8B$#`X!KRR[X!**

**KNL!VKW@ZM**

! **ZRX!**

**<2(,8B()%!J<E!3$*CC)*,!:"E!_24*,)20%!G"E!20%!H2,)C-0!>3X!H-.I2,2#(4*!"*0C(#(4(#&!-6!**

Selenastrum capricornutum **20%!** Lemna minor **!#-!"(/#**0!_*,D(8(%*CX!>,8BX!704(,-0X!H-0#2.X!**

**'-/(8-)X!KRRPX!LM@LOL**

! **LOPX!** Bayer CropScience Report Number US 0202

Page 18 of 85

# <2(,8B()%!J<E!"2II(09#-0!1HE!3$*CC)*,!:"X!>0!*8-)-9(82)!,(CT!2CC*CC.*0#!-6!#B*!I-#*0#(2)!6-,!

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! **SX!!!** Bayer CropScience Report Number US 0202

Page 19 of 85

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! **KNMSX!** Bayer CropScience Report Number US 0202

Page 20 of 85

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**Q2CB(09#-0!:HX!KRRSX!** Bayer CropScience Report Number US 0202

Page 21 of 85

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! **SOX!** Bayer CropScience Report Number US 0202

Page 22 of 85Table 1. OPP-acceptable references concerning the toxicity of bromoxynil to algae and aquatic plantsReferenceBromoxynilForm EndpointSpeciesCain and Cain (1983) Phenol 72-hour NOAEC= 22.2 mg/LC. eugamentosCullimore (1975) Phenol EC50(growth)= >0.5 mg/L and <2 mg/LHormidium barlowiFairchild et al. (1997) Phenol 96-hour NOAEC, LOAEC, EC50= 3.1, 6.3, 7.8 mg/LS. capricornutumFletcher et al. (1970) Phenol 7-day NOAEC= 6.6 mg/LC. globosaGarten (1990) Phenol 96-hour NOAEC= 1 mg/LS. capricornutumHess (1980) Phenol 48-hour NOAEC= 2.8 mg/LC. eugamentosHis and Seaman (1993) Phenol 21-day EC50= 7.5 mg/LI. galbanaMa and Liang (2001) Phenol 96-hour EC50= 4.4 mg/LC. pyrenoidosaMa et al. (2002) Phenol 96-hour EC50= 89.1mg/LC. vulgarisMa et al. (2007) Octanoate 96-hour EC50= 4.43 mg/LS. capricornutumPeterson et al. (1994) Butyratea22-hour NOAEC= 0.28 mg/LS. quadricauda,S. capricornutum,Nitzschiasp.,C. meneghiniana, M. aeruginosa, Oscillatoriasp.,Pseudanabaenasp.,A. inaequalisandA.flos-aquaePeterson et al. (1994) Butyratea7-day NOAEC= 0.28 mg/LL. minorSt-Laurent et al. (1992) Phenol 96-hour EC50(growth)= 2.9 mg/L and 3.4 mg/LbS. capricornutumaButyrate form not registered for use in the UnitedStates.bToxicity of bromoxynil tested in two different bioassay systems.Bayer CropScienceReport Number US 0202Page 23 of 85Table 2. OPP-acceptable references concerning the toxicity of bromoxynil to terrestrial plantsReference Bromoxynil Form Endpoint SpeciesAl-Khatib et al. (1992a) Phenol LOAEL= 14.2 g a.i./ha (plant injury 30 DAT)P. aviumAl-Khatib et al. (1992b) Phenol NOAEL= 142 g a.i./haM. sativaAl-Khatib et al. (1993) Phenol LOAEL= 4.2 g a.i./ha (plant injury14 DAT)V. viniferaBoutin et al. (2004) Saxo®(25% bromoxyniloctaonate)3-week EC50= 8.76 g a.i./haI.heleniumL.Clay (1972) Octanoate (25% ECformulation)LOAEL= 250 g/haF. ananassFoucart et al. (1988) Phenol and an unspecifiedesterNOAEL= 338 g a.i./ha (ester); 500 g a.i./ha(phenol)Z. maysGarten (1990) Phenol 4-week NOAEC= 1 mg/kg soilR. sativus, H. vulgareJagschitz (1979) Phenol NOAEL= 0.25 lb a.i/ha TurfgrassMartin et al. (1990) Phenol NOAEL= 550 g a.i./haT. aestivumO’Sullivan and O’Donovan (1982) Phenol NOAEL= 350 ga.i./haT. aestivum, H. vulgare,A. sativaPantone and Baker (1992) Phenol NOAEL= 300 g a.i./haO. sativaParochetti et al. (1973) Phenol NOAEL= 140 g a.i/haG. maxSandral et al. (1997) Bromoxyniln-octanoyl ester EC50= 0.18 to 0.34 g a.i./ha (depending onlife stage)T. subterraneumSchafer and Chilctoe (1970) Octanoate ED50= 2400 g a.i./haT. aestivumWax et al. (1974) Phenol LOAEL= 100 g a.i./haG. maxWilson and Burnside (1973) Phenol NOAEL= 600 g a.i./haG. maxBayer CropScienceReport Number US 0202Page 24 of 85**>##28B.*0#!K!**Bayer CropScienceReport Number US 0202Page 25 of 85BromoxynilLiteratureReviewJuly11,201112345A B C D E F**Reference Journal Paper in** **English? Full article?** **Paper Publically** **available Is** **paper the** **primary source** **of data?** Al"Khatibetal1992a WeedTechnology6(4):975"979 Y Y Y YAl"Khatibetal1992b WeedTechnology6(4):956"960 Y Y Y YAl"Khatibetal1993 WeedTechnology7(1):97"102 Y Y Y YAshtonetal.1968 WeedScience16(2):169"171 Y Y Y Y678Boutinetal.2004 Ecotoxicology13:349–369 Y Y Y YBrookeetal1984Acutetoxicitiesoforganicchemicalstofatheadminnows(Pimephalespromelas),VolI(report)Y Y Y YBuhletal1989 Arch.Environ.Contam.Toxicol.18:530"536 Y Y Y YBayer CropScienceReport Number US 0202Page 26 of 85BromoxynilLiteratureReviewJuly11,20111A B C D E F**Reference Journal Paper in** **English? Full article?** **Paper Publically** **available Is** **paper the** **primary source** **of data?** 91011Buhletal1993 Arch.Environ.Contam.Toxicol.25:152"159 Y Y Y YCainandCain1983 J.Phycol.19:301"305 Y Y Y YClarketal1996 Environ.Toxicol.Chem.15(9):1544"1550 Y Y Y Y1112131415Clay1972 Proc.11thBr.WeedControlConf.409"416 Y Y Y YCullimore1975 WeedResearch15:401"406 Y Y Y YDearandSandral1999 Au.J.Exper.Ag.39:839–847 Y Y Y YDingetal2009 BullEnvironContamToxicol83:520–524 Y Y Y NBayer CropScienceReport Number US 0202Page 27 of 85BromoxynilLiteratureReviewJuly11,20111A B C D E F**Reference Journal Paper in** **English? Full article?** **Paper Publically** **available Is** **paper the** **primary source** **of data?** 16171819DunachieandFletcher1970 Ann.Appl.Biol.66:515"520 Y Y Y YEsterandNijenstein1995 CropProtection14(5):409"413 Y Y Y YEsterandNijenstein1996 JPlantDiseasesandProtect.103(1):42"49 Y Y Y YEsterandNijenstein1996 JPlantDiseasesandProtect.103(1):42"49 Y Y Y YFairchild et al 1999USGSToxicSubstancesHydrologyProgram–Volume2of3–ContaminationofYYYN20212223Fairchildetal1999HydrologicSystemsandRelatedEcosystems(book)YYYNFairchildetal.1997Arch.Environ.Contam.Toxicol.32:353–357Y Y Y YFairchildetal.1997Arch.Environ.Contam.Toxicol.32:353–357Y Y Y YFletcheretal.1970MededelingenFaculteitLandbouwweten"schappenGent.Vol35,No.2:855"867Y Y Y YBayer CropScienceReport Number US 0202Page 28 of 85BromoxynilLiteratureReviewJuly11,20111A B C D E F**Reference Journal Paper in** **English? Full article?** **Paper Publically** **available Is** **paper the** **primary source** **of data?** 242526FollakandHurle2003a WeedResearch44:142–147 Y Y Y YFollakandHurle2003b Environ.Pollution126:139–146 Y Y Y YFollaketal.2005 Ecotoxicology14(5):503"511 Y Y Y YFoucart et al 1988Med.Fac.Landbouww.RijksunivGent.YYYY272829Foucartetal1988j53(3):1329"1334YYYYFoucartetal1988Med.Fac.Landbouww.RijksunivGent.53(3):1329"1334Y Y Y YGarten1990 PlantsforToxicityAssessment(Book) Y Y Y YBayer CropScienceReport Number US 0202Page 29 of 85BromoxynilLiteratureReviewJuly11,20111A B C D E F**Reference Journal Paper in** **English? Full article?** **Paper Publically** **available Is** **paper the** **primary source** **of data?** 30313233Garten1990 PlantsforToxicityAssessment(Book) Y Y Y YGeigeretal1988Acutetoxicitiesoforganicchemicalstofatheadminnows(Pimephalespromelas),VolIV(report)Y Y Y YHess1980 WeedScience28(5):515"520 Y Y Y YHillandCamardese1986LethalDietaryToxicitiesofEnvironmentalContaminantsandPesticidestoCoturnix(Report)Y Y Y Y3435363738Hilletal.2002 Can.J.PlantSci.82:481–484 Y Y Y YHisandSeaman1993ICES.MarineEnvironmentQuality,CopenhagenY Y Y YHisandSeaman1993ICES.MarineEnvironmentQuality,CopenhagenY Y Y YHoffmanandAlbers1984 Arch.Environ.Contam.Toxicol.13:15"27 Y Y Y YIglesiasetal2002a Ann.Appl.Biol.140:53"59 Y Y Y YBayer CropScienceReport Number US 0202Page 30 of 85BromoxynilLiteratureReviewJuly11,20111A B C D E F**Reference Journal Paper in** **English? Full article?** **Paper Publically** **available Is** **paper the** **primary source** **of data?** 3940414243Iglesiasetal2002b Int.J.PestManage.48(1):19"23 Y Y Y YIsomandWeng1977 Proceed.West.Soc.WeedSci.30:47"50 Y Y Y YIvanyetal.2000 Can.J.PlantSci.80:973–976 Y Y Y YJagschitz1979Proc.33rdAnnualMeetingNEWeedScienceSoc.Y Y Y YJudyetal.1990 Proc.South.WeedSoc.Y Y Y Y4344454647KratkyandWarren1971 WeedResearch11:257"262 Y Y Y YKratkyandWarren1971 WeedResearch11:257"262 Y Y Y YMaandLiang2001 Bull.EnvironContamToxicol67:347–351 Y Y Y YMaetal2002 Ecotox.Environ.Safe.51:128"132 Y Y Y YBayer CropScienceReport Number US 0202Page 31 of 85BromoxynilLiteratureReviewJuly11,20111A B C D E F**Reference Journal Paper in** **English? Full article?** **Paper Publically** **available Is** **paper the** **primary source** **of data?** 484950Maetal.2003 Bull.EnvironContamToxicol71:594–601 Y Y Y YMaetal.2006EcotoxicologyandEnvironmentalSafety63:456–462Y Y Y YMaetal.2007 PolishJ.ofEnviron.Stud.16(6):847"851 Y Y Y Y51525354MarshallandBirnie1985BritishCropProtect.Conf."!Weeds3:1021"1028Y Y Y YMartinetal.1990 Agron.J.82:95"97 Y Y Y YMortonandMoffett1972a Proc.WesternSoc.OfWeedSci.25:15"16 Y Y Y NMortonandMoffett1972b Environ.Entomol.1(1):102"104 Y Y Y YBayer CropScienceReport Number US 0202Page 32 of 85BromoxynilLiteratureReviewJuly11,20111A B C D E F**Reference Journal Paper in** **English? Full article?** **Paper Publically** **available Is** **paper the** **primary source** **of data?** 55565758NijensteinandEster1998 SeedSci.andTechnol.26:501"513 Y Y Y YO'Sullivan&O'Donovan1982 Can.J.PlantSci.62:445"452 Y Y Y YPantoneandBaker1992 WeedTechnology6(4):968"974 Y Y Y YParochetti1973Proc.27thAnnualMeetingNEWeedScienceSoc.75"79Y Y Y YPetersonetal.1994 AquaticToxicology28:275"292 Y Y Y Y596061Petersonetal.1994 AquaticToxicology28:275"292 Y Y Y YPetersonetal.1994 AquaticToxicology28:275"292 Y Y Y YBayer CropScienceReport Number US 0202Page 33 of 85BromoxynilLiteratureReviewJuly11,20111A B C D E F**Reference Journal Paper in** **English? Full article?** **Paper Publically** **available Is** **paper the** **primary source** **of data?** 626364656667Petersonetal.1994 AquaticToxicology28:275"292 Y Y Y YRoseberg1997 Indust.CropsandProducts6:89"96 Y Y Y YRussometal1995SARandQSARinEnvironmentalResearch4:83"95Y Y Y NSamsoe"Petersen1995 Entomophaga40(1):95"104 Y Y Y YSandraletal.1997 Au.J.Exper.Ag.37:67"74 Y Y Y YSchaferandChilctoe1970 WeedScience18(6):725"729 Y Y Y Y6768697071St"Laurentetal.1992EnvironmentalToxicologyandWaterQualityVol.7Y Y Y YSzigetietal1981 WeedResearch21:37"41 Y Y Y YVanRensburgandStrijdom1984 S."Afr.Tydskr.PlantGround1(4):135"138 Y Y Y YVillaetal2003 Environ.Toxicol.Chem.22(4):936"942 Y Y Y NBayer CropScienceReport Number US 0202Page 34 of 85BromoxynilLiteratureReviewJuly11,20111A B C D E F**Reference Journal Paper in** **English? Full article?** **Paper Publically** **available Is** **paper the** **primary source** **of data?** 7273747576Villaetal2003 Environ.Toxicol.Chem.22(4):936"942 Y Y Y NVillaetal2003 Environ.Toxicol.Chem.22(4):936"942 Y Y Y NWalshetal.1987EcotoxicologyandEnvironmentalSafety14:215"244Y Y Y YWaxetal.1974 WeedScience22(1):35"41 Y Y Y YWilsonandBurnside1973 WeedScience21(1):81"85 Y Y Y YBayer CropScienceReport Number US 0202Page 35 of 85BromoxynilLiteratureReviewJuly11,201112345G H I J K L**Methodology Organism class** **Reports biological** **effect on** **live, whole** **organisms? Age/** **life stage** Non"standard PlantPrunusaviumY Y 1to2yroldtreesNon"standard PlantMedicagosativaY Y 1to2yroldplantsNon"standard PlantVitisviniferaL. Y Y 1yroldvinesNon"standard PlantCucurbitamaximaDuchesne Y Y Seeds,seedlings**Test species** 678Non"standard Plant15non"cropspeciesinAsteraceae,Lamiaceae,PolygonaceaeandotherfamiliesY Y Seeds,seedlings,leafstage4SimilartoOECD203 FishPimephalespromelasY Y 30daysoldCommitteeonMethodsforToxicityTestswithAquaticOrganisms1975AquaticInvertebrateChironomusripariusY Y 4thinstarBayer CropScienceReport Number US 0202Page 36 of 85BromoxynilLiteratureReviewJuly11,20111G H I J K L**Methodology Organism class** **Reports biological** **effect on** **live, whole** **organisms? Age/** **life stage** **Test species** 91011SimilartoOPPTS850.1300DaphnidchronictoxicitytestAquaticInvertebrateDaphniamagnaY Y <24hoursoldneonatesSimilartoOPPTS850.5400AlgalToxicityAlgaeChlamydomonasmoewusii(Chlamydomonaseugametos)Y Y 1weekoldstockcultureNon"standard MammalFieldcommunity(Zapushudsonius,Musmusculus,Peromyscusleucopus,Microtuspennsylvanicus,Blarinabrevicauda,Sorexcinereus)Y Y Naturaldemographicdistribution1112131415Non"standard PlantFragariaananassaCambridgeFavourite Y Y 8"12leafstageNon"standard Algae 13Chlorophyceaespecies Y Y 3weekslopeculturesNon"standard PlantTrifoliumsubterraneumL.;MedicagosativaL.Y Y SeedlingsN/A AlgaeChlorellaVulgaris,RaphidocelissubcapitataY N N/ABayer CropScienceReport Number US 0202Page 37 of 85BromoxynilLiteratureReviewJuly11,20111G H I J K L**Methodology Organism class** **Reports biological** **effect on** **live, whole** **organisms? Age/** **life stage** **Test species** 16171819Non"standard BirdGallusgallusdomesticusY Y Egg/embryoNon"standard InvertebrateDerocerasreticulatumY N 400"600mgNon"standard InvertebrateDerocerasreticulatumY N 400"600mgweightNon"standard PlantLoliumperenneY Y SeedsSee Fairchild et al. 1997AlgaeSee Fairchild et al. 1997YSee Fairchild et al. 1997See Fairchild et al. 199720212223SeeFairchildetal.1997AlgaeSeeFairchildetal.1997YSeeFairchildetal.1997SeeFairchildetal.1997SimilartoOPPTS850.5400AlgalToxicity?AlgaeSelenastrumcapricornutumY Y NotreportedSimilartoOPPTS850.4400AquaticPlantToxicity?MacrophyteLemnaminorY Y NotreportedNon"standard AlgaeChlamydomonasglobosa,Chlorellapyrenoidosa,andStichococcusbacillarisY Y NotreportedBayer CropScienceReport Number US 0202Page 38 of 85BromoxynilLiteratureReviewJuly11,20111G H I J K L**Methodology Organism class** **Reports biological** **effect on** **live, whole** **organisms? Age/** **life stage** **Test species** 242526Non"standard PlantHelianthusannuusL.,cv.Frankasol Y Y 2"leafand4"leafstagesNon"standard PlantHelianthusannuus(Frankasol,Cargill,France)Y Y 2"leafand4"leafstagesNon"standard PlantHelianthusannuus(Frankasol) Y Y TwoleafstageNon standardPlantMaize (Zea mays)YY6 to 7 leaf stage272829Non"standardPlantMaize(Zeamays)YY6to7leafstageNon"standard Plant Maize(Zeamays) Y Y 6to7leafstageOECD AlgaeSelenastrumcapricornutumandChlorellavulgarisY Y 3"!or4"!dayoldculturesBayer CropScienceReport Number US 0202Page 39 of 85BromoxynilLiteratureReviewJuly11,20111G H I J K L**Methodology Organism class** **Reports biological** **effect on** **live, whole** **organisms? Age/** **life stage** **Test species** 30313233Non"standard PlantRadishes(Raphanussativusvar.Champion[Raphanusraphanistrumvar.sativus]),barley(Hordeumvulgare),bushbeans(Phaseolusvulgarisvar.BlueLake),soybeans(Glycinemaxvar.Prize)Y Y SeedtoseedlingSimilartoOECD203 FishPimephalespromelasY Y 27"44daysoldSimilartoOPPTS850.5400AlgalToxicity?AlgaeChlamydomonaseugametosY Y 3"!or4"!dayoldculturesSimilartoOECD205 BirdCoturnixcoturnixjaponicaY Y 14"dayoldPhaseolus vulgarisLSolanum tuberosumL3435363738Non"standard PlantPhaseolusvulgarisL.,SolanumtuberosumL.,BetavulgarisL.,LycopersiconesculentumMill,PhaseolusvulgarisL.Y Y 3"4leafstageSimilartoOPPTS850.1025OysterAcuteToxicityTest(shelldeposition)?AquaticInvertebrateCrassostreagigasY Y Egg/embryoNon"standard AlgaeIsochrysisgalbanaandChaetoceroscalcitransY Y NotreportedNon"standard BirdAnasplatyrhynchosY Y Egg/embryo(day3ofincubation)Non"standard InvertebrateDerocerasreticulatumY Y Egg/embryo(stageIV)Bayer CropScienceReport Number US 0202Page 40 of 85BromoxynilLiteratureReviewJuly11,20111G H I J K L**Methodology Organism class** **Reports biological** **effect on** **live, whole** **organisms? Age/** **life stage** **Test species** 3940414243Non"standard InvertebrateDerocerasreticulatumY Y Egg/embryo(stageIV)Non"standard PlantTriticumaestivum(Anza) Y Y0to1,2to3,4to5,6to7leavesandpre"bootstageNon"standard PlantTrifoliumpratenseL.,T.alexandrinumL. Y Y 1and2trifoliateleafstagesNon"standard PlantDigitariaischaemum,CyperusesculentusL. Y Ycrabgrass2"7tillerstage,nutsedge<15bladesNon"standard PlantBarley(Hordeumvulgare),soybean(Glycinemax)Y YSoybean:firsttrifoliatestage;Barley:secondleafstage4344454647Non"standard AlgaeChlorellapyrenoidosaY Y ActivelygrowingstockcultureNon"standard PlantRootbioassay:sorghum(Sorghumvulgare),oat(Avenasativa),andcucumber(Cucumissativus)Shootbioassay:sorghumandoat.Y Y pre"germinated2daysNon"standard AlgaeChlorellapyrenoidosaandScenedesmusobliquusY Y NotreportedNon"standard AlgaeChlorellavulgarisY Y NotreportedBayer CropScienceReport Number US 0202Page 41 of 85BromoxynilLiteratureReviewJuly11,20111G H I J K L**Methodology Organism class** **Reports biological** **effect on** **live, whole** **organisms? Age/** **life stage** **Test species** 484950Non"standard AlgaeScenedesmusquadricaudaY Y NotreportedNon"standard AlgaeRaphidocelissubcapitata(Selenastrumcapricornutum)Y Y NotreportedNon"standard AlgaePseudokirchneriellasubcapitata(Selenastrumcapricornutum),Scenedesmusquadricauda,Scenedesmusobliquus,Chlorellavulgaris,andChlorellapyrenoidosaY Y Notreported16 non target species (Achillea millefolium51525354Non"standard Plant16non"targetspecies(Achilleamillefolium,Anthriscussylvestris,Carduusacanthoides,Coniummaculatum,Cirsiumarvense,Convolvulusarvensis,Geraniumpusillum,Hypericumperforatum,Lamiumalbum,Ranunculusrepens,Rumexobtusifolius,Rumexsanguineus,Silenealba,Trifoliumrepens,Viciatetrasperma,Urticadioica)Y YRespectively,maturerosette,8leaves,maturerosette,6leaves,10cm,pre"flowering,flowering,3branches,flowering,flowering,13leaves,20cm,flowering,pre"flowering,4branches,22cmNon"standard PlantTriticumaestivumL. Y YStage13(3"leaf),Stage29(fullytillered/prejointing),Stage44(mid"boot)N/A Invertebrate Apismellifera Y Y NotreportedSimilartoOECDGuideline213Honeybeeacuteoraltoxicity?InvertebrateApismelliferaY Y NewlyemergedadultsBayer CropScienceReport Number US 0202Page 42 of 85BromoxynilLiteratureReviewJuly11,20111G H I J K L**Methodology Organism class** **Reports biological** **effect on** **live, whole** **organisms? Age/** **life stage** **Test species** 55565758Non"standard InvertebrateDerocerasreticulatumY N 400"600mgweightNon"standard PlantHordeumvulgareL.,TriticumaestivumL.,AvenasativaL.,BrassicacampestrisL.Y Y 4"leafstageNon"standard PlantOryzasativaL.(Lemont,Mars,Tebonnet)Y Y2"3leaf,4"5leaf,andpanicleinitiationstagesNon"standard PlantDaturastromoniumL.,Amaranthusretroflexus,Glycinemax(Kentsoybeans)Y YPostemergence(unfoliateandfirsttrifoliatestages)Non"standard AlgaeScenedesmusquadricauda,SelenastrumcapricornutumY Y Chemostatcultures596061capricornutumNon"standard CyanobacteriaMicrocystisaeruginosa,Oscillatoriasp.,Pseudanabaenasp.,Anabaenainaequalis,andAphanizomenonflos"aquaeY Y ChemostatculturesNon"standard AlgaeNitzschiasp.andCyclotellameneghinianaY Y ChemostatculturesBayer CropScienceReport Number US 0202Page 43 of 85BromoxynilLiteratureReviewJuly11,20111G H I J K L**Methodology Organism class** **Reports biological** **effect on** **live, whole** **organisms? Age/** **life stage** **Test species** 626364656667Non"standard MacrophyteLemnaminorY Y NotreportedNon"standard PlantVernoniagalamensis(Cass.)Less.Y Y50"150mmtallforgreenhouseexperiment,25"65mmtall&3"6leavesforfieldtestNon"standard Multiple MultipleN N NotreportedNon"standard InvertebrateAleocharabilineataY Y Adult,matedfemalesNon"standard PlantTrifoliumsubterraneumL. Y Y2cultivarsat3"4and8"10trifoliateleafstagesNon"standard PlantTriticumaestivumL.var.Nugaines,Amsinckia intermedia Fish. & MeyY YWheatat2"tiller,andfiddleneckat12"14"leaf stage6768697071AmsinckiaintermediaFish.&MeyleafstageSimilartoOPPTS850.5400AlgalToxicity?AlgaeSelenastrumcapricornutumY Y 4"7dayoldcellsNon"standard PlantTriticumaestivumY Y 7"daysoldNon"standard Plant Lucerne(Medicagosativa) Y Y GerminatedseedsNotreported Algae Notreported N Y NotreportedBayer CropScienceReport Number US 0202Page 44 of 85BromoxynilLiteratureReviewJuly11,20111G H I J K L**Methodology Organism class** **Reports biological** **effect on** **live, whole** **organisms? Age/** **life stage** **Test species** 7273747576NotreportedAquaticInvertebrateDaphniamagnaY Y NotreportedNotreported Fish Notreported N Y NotreportedNon"standard AlgaeSkeletonemacostatum,Thalassiosirapseudonana,andChlorellasp.Y Y NotreportedNon"standard PlantGlycinemax(L.)Merr.Y YPostemergencefieldexperiments,greenhouseexperimentsatfirsttrifoliateNon"standard PlantSoybean(Glycinemax)andvariousassociatedweedspeciesY YGreenhouse:8"23cmheightsoybeanplants(weedspeciesbetween3and15cm);Field:notspecifiedBayer CropScienceReport Number US 0202Page 45 of 85BromoxynilLiteratureReviewJuly11,201112345M N O P Q R S**Source of** **test species** **Number of** **replicates Number** **of organisms/** **replicate**PlantedatIrrigatedAgricultureandExtensionCenter,Prosser,WashingtonField(testplots) YRates:4.26,14.2,42.6,142g/haY 3 2PlantedatIrrigatedAgricultureandExtensionCenter,Prosser,WashingtonField(testplots) Y Rates:4,14,43,142g/ha Y 4 NotreportedPlantedatIrrigatedAgricultureandExtensionCenter,Prosser,WashingtonField(testplots) Y Rates:4.3,14.2,42.6,142g/ha Y 3 2Seeds,notspecified Laboratory Y10"4MY 4 2**Location: field** **or laboratory?** **Concentrations tested** 678SeedsuppliersinUS/Canada,2speciescollectedinDenmarkLaboratory(greenhouse) YRange"findingand40,72,130,240g/haY 6 1USEPAlabatDuluth Laboratory Y2.47,3.80,5.85,9.0,and13.8mg/LY 2 20NationalFisheriesContaminantResearchCenter,YanktonSDLaboratory YLogseriesof6"8unspecifiedconcentrationsY Notreported 10Bayer CropScienceReport Number US 0202Page 46 of 85BromoxynilLiteratureReviewJuly11,20111M N O P Q R S**Source of** **test species** **Number of** **replicates Number** **of organisms/** **replicate Location:** **field or** **laboratory? Concentrations** **tested**91011NotreportedLaboratory Y5,10,20,40,80μg/Lincontinuousexposureorasdailypulseswhichgavethesamerangeof24"haverageconcentrationsY 4 15CultureCollectionofAlgaeatUniversityofTexasLaboratory Y1,2.5,5,7.5,10,15,20,30,40,50,60,and80μM(or0.3,0.7,1.4,2,2.8,4.2,5.5,8.3,11.1,13.8,16.6,and22.2mg/LassumingMW=276.9)Y 62x106cells/plateFieldField Y 0.57kga.i./ha Y 6pairedplots N/A1112131415Runners,notspecified Laboratory(greenhouse) Y Rates:0.25,1.0kg/ha Y 2 1NationalCollectionofAlgaeandProtozoa,Cambridge,U.K.Laboratory Y0.01,0.02,0.05,0.1,0.2,0.5,1,2,5,and10mg/LY 5 NotreportedSownatAgriculturalInstitute,WaggaWagga,NSWField(testplots) Y Rate:200ga.i./Lat1.4L/ha Y 3 1.8x6mplotsN/A N/A N N/A N N/A N/ABayer CropScienceReport Number US 0202Page 47 of 85BromoxynilLiteratureReviewJuly11,20111M N O P Q R S**Source of** **test species** **Number of** **replicates Number** **of organisms/** **replicate Location:** **field or** **laboratory? Concentrations** **tested**16171819Notreported Notreported.Laboratory? N 10,25,50,100ppm Y Notreported N/ACloverfieldatExperimentalStationLelystadLaboratory YBromoxynil225ECat0.9,1.8,2.7ga.i./kgseedBromoxynil250SCat0.25,0.50,0.75and1.00ga.i./kgseedY 4 3CloverfieldatExperimentalStationLelystadLaboratory Y 5ga.i./kgseed Y 4 3CloverfieldatExperimentalStationLelystadLaboratory Y 5ga.i./kgseed Y 3 50See Fairchild et al. 1997See Fairchild et al. 1997NSee Fairchild et al. 1997NSee Fairchild et al. 1997See Fairchild et al. 199720212223SeeFairchildetal.1997SeeFairchildetal.1997NSeeFairchildetal.1997NSeeFairchildetal.1997SeeFairchildetal.1997CarolinaBiologicalSupplyCompany Laboratory Y5concs.dilutedina50%seriesfromtechnicalgrade(conc.notspecified)N 32x104cells/mLCarolinaBiologicalSupplyCompany Laboratory Y5concs.dilutedina50%seriesfromtechnicalgrade(conc.notspecified)N 3 12frondsCultureCollectionofAlgaeandProtozoa,UniversityofCambridgeLaboratory Y1.19x10"5M,2.38x10"5M,and3.57x10"5M(3.3,6.6,9.89mg/L,assumingMW=276.9)Y 3Notreported,1mg/LalgaesolutionBayer CropScienceReport Number US 0202Page 48 of 85BromoxynilLiteratureReviewJuly11,20111M N O P Q R S**Source of** **test species** **Number of** **replicates Number** **of organisms/** **replicate Location:** **field or** **laboratory? Concentrations** **tested**242526PlantedandcultivatedingreenhouseLaboratory(windtunnel/greenhouse)YConcentrationsbetween0.012"4.104ug/m3Y 6 1PlantedandcultivatedingreenhouseLaboratory(windtunnel/greenhouse)Y17concentrationsbetween0.012"4.104ug/m3Y 6 1Cargill,France Laboratory(greenhouse) YEightconcentrationsbetween0.120and2.218μg/m3Y 8 1Not reportedFieldY338 g/haY115272829NotreportedFieldY338g/haY115NotreportedField Y 500g/ha Y 1 15CarolinaBiologicalSupplyCompany Laboratory Y0.01,0.1,1,10,and100mg/LofgrowthmediumY 31x106cellsper100mLtestBayer CropScienceReport Number US 0202Page 49 of 85BromoxynilLiteratureReviewJuly11,20111M N O P Q R S**Source of** **test species** **Number of** **replicates Number** **of organisms/** **replicate Location:** **field or** **laboratory? Concentrations** **tested**30313233Notreported Laboratory(greenhouse) Y0.01,0.1,1,10,and100mg/kginsoilY 36(plantsweededto6aftergermination)USEPAlabatDuluthandUniversityofWisconsin"SuperiorLaboratory Y4.43,6.82,10.5,16.1and24.8mg/LY 2 20IndianaculturecollectionNo.9 Laboratory Y1E"8,1E"7,1E"6,1E"5,1E"4,1E"3M(0.003,0.03,0.28,2.8,27.7,276.9mg/L,assumingaMWof276.9)Y 41x105cells/mLPatuxentWildlifeResearchCenter,LaurelMDLaboratory(indoorpens) Y 1000,2236,5000ppminfeed Y 1 141potatoperpot,2dry3435363738Growninpots Laboratory(bioassay) YMixtureof4herbicidesincludingbromoxynilat0.5g/haY 4to7potsp p p , ybeansperpot,or6plantsperpotforsugarbeets,sunflowers,tomatoesNotreportedLaboratory Y0(controls),0.025,0.05,0.1,0.25,0.5,l,2.5,5,and10mg/LYUnclear(200totallarvaegrowthand50totalforcelldeposition)NotreportedStocksatM.A.F.F.ConwyinWales Laboratory Y0(controls),0.025,0.05,0.1,0.25,0.5,l,2.5,5,and10mg/LY Notreported NotreportedWhistlingWings,HanoverILandOakRidgeGameFarm,Gravette,ARLaboratory Y3"6geometricallyspacedconcentrationsY 30 1eggCloverfieldsinSantiagodeCompostela(Spain)andLelystad(Netherlands)Laboratory Y 5inatwo"foldgeometricseriesY 5 5eggsBayer CropScienceReport Number US 0202Page 50 of 85BromoxynilLiteratureReviewJuly11,20111M N O P Q R S**Source of** **test species** **Number of** **replicates Number** **of organisms/** **replicate Location:** **field or** **laboratory? Concentrations** **tested**3940414243CloverfieldsinSantiagodeCompostela(Spain)andLelystad(Netherlands)Laboratory Y5ingeometricseriesrangingfrom0.000393to3.93mga.i./cm2Y 3 5eggsNotreportedField Y 0.5lbsperacre Y 4 UnclearFromseedField(testplots) Y Rate:300ga.i./ha Y 3 0.25x0.25mquadratsGrowinginnaturaloutdoorfieldsField(existingfootballfieldandsodfarm)Y Rates:0.25and0.5lba.i./acre Y 3 4x5'plotsNotreportedLaboratory Y 0.1%Buctril Y 10 5plants(measuredtwice)4344454647NotreportedLaboratory Y 1and10ppm Y 4 density0.02"0.06NotreportedLaboratory Y 1and10ppm Y 4Rootbioassay:4seedsShootbioassay:5seedsInstituteofWuhanHydrobiology,theChineseAcademicofScienceLaboratory Y 0to150mg/L Y NotreportedChlorella:2x105cells/LScenedesmus:3x105cells/LInstituteofWuhanHydrobiology,ChineseAcademyofScienceLaboratory YUnspecifiedrangingfrom0to150mg/LY 3 8E5cellcountBayer CropScienceReport Number US 0202Page 51 of 85BromoxynilLiteratureReviewJuly11,20111M N O P Q R S**Source of** **test species** **Number of** **replicates Number** **of organisms/** **replicate Location:** **field or** **laboratory? Concentrations** **tested**484950InstituteofWuhanHydrobiology,theChineseAcademicofScienceLaboratory Y Variousconcentrations N 3Opticaldensity(OD608nm)=0.05InstituteofWuhanHydrobiology,theChineseAcademicofScienceLaboratory Y Widerangeofconcentrations N 35x104algalcells/mLInstituteofWuhanHydrobiology,theChineseAcademyofScienceLaboratory YTestsinaconcentrationseriesof50%dilutionsbetweentherangesof:0.2"20mg/L(C.vulgaris),50"1,000mg/L(C.pyrenoidosa),0.5"50mg/L(S.obliquus),0.1"20mg/L(S.quadricauda),and0.2"20mg/L(P.subcapitata).Y 3 20mLof5x105cells/mL51525354NotreportedLaboratory Y 0.76kga.i./ha Y 2 1ofeachspeciesSeededatUWyomingResearch&ExtensionCenter,TorringtonWYField(testplots),andlaboratory(grainanalysis)Y Rate:0.55kg/ha Y 6forfield,4forlab 2x6mplotsNotreportedN/A N N/A N N/A N/ANotreportedLaboratory Y10,100,1000ppminsucrosesyrupY 5 Approx.100beesBayer CropScienceReport Number US 0202Page 52 of 85BromoxynilLiteratureReviewJuly11,20111M N O P Q R S**Source of** **test species** **Number of** **replicates Number** **of organisms/** **replicate Location:** **field or** **laboratory? Concentrations** **tested**55565758CloverfieldatExperimentalStationLelystadLaboratory Y 5,25,50,100ga.i./kgseeds Y 4 3to5Notreported Field(testplots) Y Rate:0.35kg/ha Y 3 2x6mplotsDrill"seededatRiceResearchStation,CrowleyLAField(testplots) Y Rates:0.3and0.6kga.i./ha Y 4 2.1x7.6mplotsSeededandplantedatWyeInstitute,QueenstownMDField(testplots) YRates:0.140"1.12kg/ha(entireplant,andlowerthird)Y 1 NotknownUnspecifiedlaboratorycultures Laboratory Y 0.280mg/L Y 3S.quadricauda:25x104cells/mLS capricornutum: 63 x 104596061S.capricornutum:63x104cells/mLUnspecifiedlaboratorycultures;PasteurInstituteCultureCollection,Paris,France;andUniversityofTorontoCultureCollection,Toronto,Ontario,Canada.Laboratory Y 0.280mg/L Y 3M.aeruginosa:117"124x104cells/mLP.sp.:1,326x104cells/mLO.sp.:548x104cells/mLA.inaequalis:102x104cells/mLA.flos"aquae:63x104cells/mLUniversityofTexasCultureCollection,Austin,Texas,USA,andFreshwaterInstituteCultureCollection,Winnipeg,Manitoba,Canada.Laboratory Y 0.280mg/LY 3N.sp.:26x104cells/mLC.meneghiniana:27x104cells/mLBayer CropScienceReport Number US 0202Page 53 of 85BromoxynilLiteratureReviewJuly11,20111M N O P Q R S**Source of** **test species** **Number of** **replicates Number** **of organisms/** **replicate Location:** **field or** **laboratory? Concentrations** **tested**626364656667SmallmanmadepondnearSaskatoon,SaskatchewanLaboratory Y 0.280mg/LY 4 3leavesPlantedinflatsforgreenhouse,andplantedbyseedforfieldexperimentFieldplotsandlaboratory(greenhouse)YRate:0.28kga.i./haforgreenhouseandfieldY 2(forfield)6.1x2mplots,0.46x0.91mflatsN/AN/A N N/A N N/A N/ANotreportedLaboratory Y ~0.6μL/cm2 Y 9 1Plantedfromseedingreenhouse Laboratory(greenhouse) YRates:0,0.14,0.28,0.42,0.56,0.70ga.i./haY 2 5Plantedcaryopses,thinnedtooneseedlingper potLaboratory(controlledtestchambers)YRates:9"288ga.i./haforfiddleneck,1100"66200ga.i./haY 10 16768697071perpotchambers)forwheatNotreportedLaboratory YUnspecified,10concentrationsintheinhibitionrangeusingastocksolutiondilution(7.5g/Lin100%acetone)NMicroplate:5testand10controlFlask:3testand6controlNotreportedNotreportedLaboratory Y 30μmolsolution Y 27 UnclearNotreportedLaboratory Y 2.5L/ha Y 4to5 NotreportedNotreportedLaboratory Y Notreported N Notreported NotreportedBayer CropScienceReport Number US 0202Page 54 of 85BromoxynilLiteratureReviewJuly11,20111M N O P Q R S**Source of** **test species** **Number of** **replicates Number** **of organisms/** **replicate Location:** **field or** **laboratory? Concentrations** **tested**7273747576NotreportedLaboratory Y Notreported N Notreported NotreportedNotreportedLaboratory Y Notreported N Notreported NotreportedUniversityofRhodeIslandcollection,WoodsHoleOceanographicInstitution,andCultureCollectionofAlgaefromUniversityofTexasatAustin.Laboratory YNotreported(highesttestedconcentrationdidnotexceedsolubility)Y 2 4.7x104cells/mLPlantedfromseedatAgronomySouthFarm,UrbanaILFieldplotsandlaboratory(greenhouse)Y Rates:0.07,0.1,0.2,0.3kg/ha Y 3"4 VariesdependingontestPlantedinpotsandinfieldFieldplotsandlaboratory(greenhouse)YRates:0.6kg/ha(fieldstudy),1.1kg/ha(greenhousestudy) Y44.5x15mand4.5x7.5mplots(field),4"6/pot(greenhouse)Bayer CropScienceReport Number US 0202Page 55 of 85BromoxynilLiteratureReviewJuly11,201112345T U V W X Y Z AA**Single chemical** **exposure? Source** **of bromoxynil** **Bromoxynil formulation** **Solvent**2applications,1"yrapartY Y Water+0.25%surfactant Y Notreported Notreported X"77surfactant2applications,1"yrapartY Y Water+0.25%surfactant Y Notreported Notreported X"77surfactant3applicationsover2yearsY Y Water+0.25%surfactant Y Notreported Notreported X"77surfactantSeedssoakedfor4hrsinherbicidesolutionY YWaterandenzymepreparationfrom"clean"plantsY Notreported Notreported Notreported**Duration of** **exposure? Types** **of controls/** **acceptability of** **control**678Singlesprayevent Y Y Negativecontrol? Y NotreportedSaxo(20%octanoate"bromoxynil)Notreported96hours Y Y Negativecontrol Y AldrichChemicalCo.Analyticalgrade(>98%pure)None48hr Y YSolventandnegativecontrolsY UnionCarbideME4Brominal(34.9%bromoxynil)AcetoneBayer CropScienceReport Number US 0202Page 56 of 85BromoxynilLiteratureReviewJuly11,20111T U V W X Y Z AA**Single chemical** **exposure? Source** **of bromoxynil** **Bromoxynil formulation** **Solvent Duration** **of exposure?** **Types of** **controls/ acceptability** **of control** 9101128d(continuousandpulsedexposures)Y Y Solventcontrol YRhone"PouleneAgCo.,ResearchTrianglePark,NCBuctril(33.4%bromoxynil) Acetone7days(3daysforreproductionendpoint)Y Y Negativecontrol Y Notreported Techgrade95% NoneOnefallapplication88and89;twospringapplicationsin90and91N N Negativecontrol Y UnionCarbideBrominal(28.5%bromoxyniland28.5%MCPA)None1112131415Singletreatment Y Y Yes,butnotspecified Y Notreported 25%e.c.octanoateester Notreported5,10,20,and30days Y Y Negativecontrol Y AmchemProductsInc.,Amber Analyticalgrade None2applications,1"yrapartY Y Unsprayedcontrol Y BrominilBromoxyniln"octanoylesterNotspecifiedN/A N N/A N/A N N/A N/A N/ABayer CropScienceReport Number US 0202Page 57 of 85BromoxynilLiteratureReviewJuly11,20111T U V W X Y Z AA**Single chemical** **exposure? Source** **of bromoxynil** **Bromoxynil formulation** **Solvent Duration** **of exposure?** **Types of** **controls/ acceptability** **of control** 16171819Singleinjection Y Y Notreported N NotreportedNotreported.Analyticalgrade?Acetone7days YN(pre"treatedwithanunidentifiedfungicide)Fungicide"onlycontrol Y NotreportedBromoxynil225ECandBromoxynil250SCN/A,seedswerepretreatedwithfungicide6days Y Y Negativecontrol Y Notreported Bromoxynil250SC None28days Y Y Negativecontrol Y Notreported Bromoxynil250SC NoneSeeFairchildetal.NSeeFairchildetal.See Fairchild et al. 1997NSee Fairchild et al. 1997See Fairchild et al. 1997SeeFairchildetal.202122231997N1997SeeFairchildetal.1997NSeeFairchildetal.1997SeeFairchildetal.1997199796hrs Y YSolventandnegativecontrolsY Notreported TechnicalgradeAcetoneorwaterdilution96hrs Y YSolventandnegativecontrolsY Notreported TechnicalgradeAcetoneorwaterdilution7days Y Y Negativecontrol Y May&BakerLtd. sodiumsalts NoneBayer CropScienceReport Number US 0202Page 58 of 85BromoxynilLiteratureReviewJuly11,20111T U V W X Y Z AA**Single chemical** **exposure? Source** **of bromoxynil** **Bromoxynil formulation** **Solvent Duration** **of exposure?** **Types of** **controls/ acceptability** **of control** 24252624hr Y Y Controlplantsnotexposed N Spiess"UraniaChem.,GmbHCertrolB(bromoxyniloctanoateester)No24hr Y Y Controlplantsnotexposed N Spiess"UraniaChem.,GmbHCertrolB(bromoxyniloctanoateester)No24hours Y Y Negativecontrol N Spiess"UraniaChemicalsCertrolB(bromoxynil"octanoate)NoneApplicationsonday1YYNegative controlYNot reportedBromoxynil esterNone272829pp yand6YYNegativecontrolYNotreportedBromoxynilesterNoneApplicationsonday1and6Y Y Negativecontrol Y Notreported Bromoxynilphenol None24and96hr Y Y Negativecontrol Y Aldrich 98%activeingred.NoneBayer CropScienceReport Number US 0202Page 59 of 85BromoxynilLiteratureReviewJuly11,20111T U V W X Y Z AA**Single chemical** **exposure? Source** **of bromoxynil** **Bromoxynil formulation** **Solvent Duration** **of exposure?** **Types of** **controls/ acceptability** **of control** 303132334weeks Y Y Negativecontrol Y Aldrich 98%activeingred.None96hours Y Y Negativecontrol Y AldrichChemicalCo.Analyticalgrade(>98%pure)None48hr Y YSolventandnegativecontrolsY Notreported Technicalgradeorbetter NoneorDMSO5days N N Solventcontrol YCommercialchemicalretailerormanufacturerBronate(31.7%bromoxynil,34%MCPA)CornoilNotreported(appliedas3435363738Singletreatment Y N Solventcontrol Y Notreportedp ( ppanherbicidemixturealongwithMCPA,2,4"D,anddicamba)Ethanol9days Y Y Solventcontrol Y NotreportedBromoxynil(puritynotreported)Acetone?21days Y Y Solventcontrol Y NotreportedBromoxynil(puritynotreported)Acetone?Immersioninsolutionfor30sY NNegativeand"solvent"(e.g.un"immersedeggsandeggsimmersedinwateronly)Y Rhone"poulene,IncBronate(bromoxyniloctanoate+MCPA)None7days Y Y Negativecontrol Y Notreported Bromoxynil250g/LSC NoneBayer CropScienceReport Number US 0202Page 60 of 85BromoxynilLiteratureReviewJuly11,20111T U V W X Y Z AA**Single chemical** **exposure? Source** **of bromoxynil** **Bromoxynil formulation** **Solvent Duration** **of exposure?** **Types of** **controls/ acceptability** **of control** 394041424320days Y Y Negativecontrol Y Notreported Bromoxynil250g/LSC NoneOneapplication Y Y Negativecontrol Y Notreported Notreported None3applications,1"yrapartY Y Negativecontrol Y Notreported Notreported NotreportedSingletreatment Y Y Negativecontrol Y Notreported3,5"dibromo"4"hydroxybenzonitrileWaterSinglesprayapplicationY Y Negativecontrol Y Notreported Buctril None434445464718"36hrs Y Y Negativecontrol Y Notreported Technicalgrade NoneRootbioassay:2daysShootbioassay:4daysY Y Negativecontrol Y Notreported Technicalgrade None96hrs Y Y Negativecontrol Y Notreported Notreported None96hours Y YSolventandnegativecontrolsY PurchasedinChinaTechnicalgrade(95%pure)Unclear(potentiallyacetoneormethanol)Bayer CropScienceReport Number US 0202Page 61 of 85BromoxynilLiteratureReviewJuly11,20111T U V W X Y Z AA**Single chemical** **exposure? Source** **of bromoxynil** **Bromoxynil formulation** **Solvent Duration** **of exposure?** **Types of** **controls/ acceptability** **of control** 48495096hrs Y Y "Appropriate"controls Y Notreported 95%technicalproduct<0.05%acetoneordistilledwater96hrs Y Y Negativecontrol? Y People'sRepublicofChina 95%technicalproduct<0.05%acetoneordistilledwater96hrs Y Y Solventcontrol Y LiminChemicalCo.Ltd.,ChinaBromoxyniloctanoate96%technicalproduct<0.05%acetoneordistilledwater51525354Twoapplications Y N Negativecontrol Y NotreportedBromoxynil+ioxynil(esterEC)None3applications,1ateachgrowthstageY Y Negativecontrol Y Notreported Notreported NotreportedN/A N Y Notreported N Notreported Notreported Notreported60days Y Y Negativecontrol N NotreportedBromoxynil("purestformavailable")NoneBayer CropScienceReport Number US 0202Page 62 of 85BromoxynilLiteratureReviewJuly11,20111T U V W X Y Z AA**Single chemical** **exposure? Source** **of bromoxynil** **Bromoxynil formulation** **Solvent Duration** **of exposure?** **Types of** **controls/ acceptability** **of control** 555657587days Y Y Negativecontrol? Y/N Notreported Bromoxynil250SC None1treatment Y Y Negativecontrol Y Notreported NotreportedTween20surfactant3applications,1ateachgrowthstageY Y Negativecontrol Y Notreported Notreported Notreported2applicationsateachgrowthstage(1usingeachmethod)Y YYes,cultivatedandnon"cultivatedY Notreported Notreported Notreported22hrs Y Y Negativecontrol Y NotreportedBromoxynilbutyrateester,technical materialNone596061technicalmaterial22hrs Y Y Negativecontrol Y NotreportedBromoxynilbutyrateester,technicalmaterialNone22hrs Y Y Negativecontrol Y NotreportedBromoxynilbutyrateester,technicalmaterialNoneBayer CropScienceReport Number US 0202Page 63 of 85BromoxynilLiteratureReviewJuly11,20111T U V W X Y Z AA**Single chemical** **exposure? Source** **of bromoxynil** **Bromoxynil formulation** **Solvent Duration** **of exposure?** **Types of** **controls/ acceptability** **of control** 6263646566677days Y Y Negativecontrol Y NotreportedBromoxynilbutyrateester,technicalmaterialNone1application Y Y Hand"weededcontrol Y Notreported Notreported NotreportedN/A N N N/A N N/A N/A N/A19days Y Y Negativecontrol Y NotreportedBromoxynil(60%)+CGA1136872(surfactant?)None2applicationsat2leafstagesY Y Negativecontrol Y NotreportedBromoxyniln"octanoylester(200ga.i./L)Notreported1application Y Y Acetone(90%)control Y Notreported BromoxyniloctanoateIn90%acetonev/v6768697071v/v96hrs Y YSolventandnegativecontrolsY Rhone"PoulencAgriculture Technicalgrade Acetone2hours Y YSolventandnegativecontrols?Y/NSynthesizedatResearchCentreofCHINOINPharmaceuticalandChemicalWorksLtd.Pure(notspecified) DMSO6weeks Y YRhizobiuminoculatedandnon"inoculatedcontrolsY Notreported BrominalEC None96"hour Y Y Notreported N NotreportedNotreported.Analyticalgrade?NotreportedBayer CropScienceReport Number US 0202Page 64 of 85BromoxynilLiteratureReviewJuly11,20111T U V W X Y Z AA**Single chemical** **exposure? Source** **of bromoxynil** **Bromoxynil formulation** **Solvent Duration** **of exposure?** **Types of** **controls/ acceptability** **of control** 727374757648"hour Y Y Notreported N NotreportedNotreported.Analyticalgrade?Notreported96"hour Y Y Notreported N NotreportedNotreported.Analyticalgrade?Notreported72hr(S.costatumandT.pseudonana)96hr(Chlorellasp)Y YSolventandnegativecontrolsYAldrichChemicalCo.(Wilwaukee,WI)3,5"dibromo"4"hydroxybenzonitrileNanogradeacetone1application Y YWeedyandhand"weededcontrolsY Notreported3,5"dibromo"4"hydroxybenzonitrileNotreported1application Y YCultivatedandhand"weededcontrols YNotreported Notreported NotreportedBayer CropScienceReport Number US 0202Page 65 of 85BromoxynilLiteratureReviewJuly11,201112345AB AC AD AE AF AG**Chemical analysis?** **Statistics Results Score (1** **" 3)** NoInterpretedNOAELsandLOAELs(growth)YANOVA,meansseparatedbyLSDp=0.050"12%injury30DAT;95"102%ofheightachieved;For1yroldtrees:LOAEL30DAT=14.2g/ha;NOAEL120DAT=142g/ha;For2yroldtrees:LOAEL30DAT=142g/ha;Fortreeheight(1yroldtrees):NOAEL=142g/ha2NoInterpretedNOAELsandLOAELs(growth,yield,injury)YANOVA,meansseparatedbyLSDp=0.0598"101%ofheightachieved;NOAEL=142g/haforgrowth,yield,injury2NoInterpretedLOAEL(growthandinjury)YANOVA,meansseparatedbyLSDp=0.050"57%injury;67to110%caneweightachieved;LOAEL14DATforpercentinjury=4.3!"!14.2g/ha2NoInterpretedLOAEL(proteolyticactivity)Y Averages,notspecified32%reducedproteolyticactivity,ClassIIgrowthinhibition;LOAEL=10"4M3LOAELforrange"finding,EC50(listedforvarious**Paper reports** **a calculated** **endpoint?**678Yes,ofstocksolutionsEC50,vegetativevigorafter3weeksY LinearinterpolationspeciesinAppendix),EC50srangedfrm8.76(Inulahelenium)!"77.84(Menthaspicata)ga.i./ha.1Yes(daily) 96"hourLC50 YTrimmedSpearman"KarbermethodLC50=11.5mg/L;noobvioussignsofdistressobservedpriortodeath1No24"hrand48"hrEC50(immobilization)Y Litchfield"Wilcoxonmethod24"hrEC50=2.35mg/L48"hrEC50=1.90mg/L2Bayer CropScienceReport Number US 0202Page 66 of 85BromoxynilLiteratureReviewJuly11,20111AB AC AD AE AF AG**Chemical analysis?** **Statistics Results Score (1** **" 3)** **Paper reports** **a calculated** **endpoint?**91011NoNOAEC,LOAEC,MATCforgrowth,survival,reproduction,populationgrowthYOnewayANOVA,movingaverageanglemethodContinuousexposure:NOAEC=20μg/L;LOAEC(growth,reproduction,pop.growth)=40μg/L;MATC(geo.mean)=28μg/LIntermittentexposure:NOAEC=~10μg/L;LOAEC(growth,reproduction,pop.growth)=20μg/L;MATC(geo.mean)=14μg/L2NotreportedNOAEC(growthandreproduction)Y ANOVANOAEC(growthandreproduction)=80μM(22.2mg/L)2No No NPopulationestimateswithCAPTUREandJOLLYprogramsOpaquecorneasinvolescorrelatedtoBrominalexposure31112131415NoInterpreted8"weekLOAEL(vegetativevigor,growth)Y Nonereported40%reducedweight,reducedvigor;LOAELforweight=0.25kg/ha2NotreportedNOAEC,LOAEC,EC50(growthrate,growthinhibition)GraphicalinterpretationNOAEC=10mg/LforallspeciesexceptH.barlowiH.barlowi:EC50(growth)=0.5"2mg/L;LOAEC(growthinhibition)=5mg/L2NoLOAEL(injury,yield)30"60DATY ANOVACloverLOAEL(herbageyield)/lucerneLOAEL(yield)=280ga.i/ha(200ga.i./Lappliedat1.4L/ha).3N/A No N N/A N/A3Bayer CropScienceReport Number US 0202Page 67 of 85BromoxynilLiteratureReviewJuly11,20111AB AC AD AE AF AG**Chemical analysis?** **Statistics Results Score (1** **" 3)** **Paper reports** **a calculated** **endpoint?**16171819No No N NoneInjectionof10and25ppmbromoxynilinacetonereducedhatchingto64and69%ofcontrols,respectively.Injectionof50ppmandgreatercaused100%eggmortality3No No N LSDReducedconsumptionofbromoxynil"exposedseeds3Notreported No N None(percentages)Bromoxynilseedtreatmentdidnotpreventslugsfromdamagingseedlings3No No N None(percentages)63to67%reductioningerminationfollowingbromoxynilseedtreatment.3SeeFairchildetal.See Fairchild et al. 1997NSee Fairchild et al. 1997See Fairchild et al. 19973202122231997SeeFairchildetal.1997NSeeFairchildetal.1997SeeFairchildetal.19973NoEC50,NOAEC,LOAEC(growth)YANOVA,Dunnett'smultiplerangetestEC50=7,762μg/L,NOAEC=3,125μg/L,LOAEC=6,250μg/L2No EC50,LOAEC(growth) YANOVA,Dunnett'smultiplerangetestEC50=8,065μg/L,LOAEC=3,125μg/L 3No InterpretedNOAEC(growth) Y ANOVANOAEC=3.57x10"5M(S.bacillaris,C.pyrenoidosa);2.38x10"5(C.globosa).C.globosaandS.bacillarismoresensitivethanC.pyrenoidosa;bromoxynilwasmoretoxicatpH5(vs.6.5and8.0)2Bayer CropScienceReport Number US 0202Page 68 of 85BromoxynilLiteratureReviewJuly11,20111AB AC AD AE AF AG**Chemical analysis?** **Statistics Results Score (1** **" 3)** **Paper reports** **a calculated** **endpoint?**242526YesLOAEL/NOAEL,ED50(quantumyield,leafinhibition,biomass)Y Non"linearregression,ANOVALOAEL=0.78μg/m3forquantumyield,ED50=1.443μg/m3forleafinhibition,NOAEL=1.199μg/m3forbiomassat2"leafstage,NOAEL=1.202μg/m3for4"leaf;NOAELforquantumyield=0.265μg/m33YesLOAEL/NOAEL,ED50(quantumyield,leafinhibition,biomass)Y Non"linearregression,ANOVAED50sforexposedleaves0.9164DATto1.562μg/m316DAT.ED50sofnewlydevelopedleaves1.443to2.126μg/m3,2and8DAT.Completerecoveryin16daysat1.310μg/m3;NOAELforexposedleaves0.265ug/m33YesNOAEL;ED50(quantumyield)Y Non"linearregressionanalysisExposedleaves(quantumyield):NOAEL=0.449μg/m3;EC50=1.106μg/m3Leavesdevelopedafterexposure(quantumyield):NOAEL=1.159μg/m3;EC50=1.588μg/m3.Allexposuresdelayedflowering.3NoInterpretedNOAEL(growth,YNoneNOAEL=338g/ha;Noeffectonmaizeyield2272829Nop (g ,yield)YNoneg/ ; ywhenappliedfornightshadecontrol2No InterpretedNOAEL(yield) Y NoneNOAEL=500g/ha;Noeffectonmaizeyieldwhenappliedfornightshadecontrol2NotreportedInterpretedNOAEC(growthrate)Y ANOVA 96"hourNOAEC=1mg/L 1Bayer CropScienceReport Number US 0202Page 69 of 85BromoxynilLiteratureReviewJuly11,20111AB AC AD AE AF AG**Chemical analysis?** **Statistics Results Score (1** **" 3)** **Paper reports** **a calculated** **endpoint?**30313233NotreportedInterpretedNOAEL(growthrate)Y ANOVA NOAEL=1mg/kg(radish&barley) 2Yes(daily) 96"hourLC50 YTrimmedSpearman"KarbermethodLC50=13.8mg/L;lossofschoolingbehavior,hyperactivityandincreasedrespirationwereobserved1NotreportedInterpretedNOAECandLOAEC(growth)Y Duncan'smultiplerangetestNOAEC=1x10"5(nosignificantinhibitionofgrowth);LOAEC=significantlyinhibitedgrowthat1x10"4M2No 5"daydietaryLD50 Y NoneLD50>5000ppmdiet(reducedfoodconsumptionobserved)3Potato:NOAEL(leafarea,dryweight,damage)=3435363738NoInterpretedNOAEL(leafarea,weight,damage)Y Fisher'sLSDtest( , y g , g )2.25g/ha;Sugarbeet,sunflower:NOAEL(leafarea,dryweight)=2.25g/ha;Tomato:NOAEL(dryweight)=2.25g/ha3No9dayLC50;9"dayEC10(shelldeposition)Y Notreported9dayLC50=7.0mg/L;9dayEC10(shelldeposition)=0.80mg/L2No21dayEC20(growth);NOAEC(growth)Y NotreportedI.galbana:21dayEC20(growth)=7.5mg/L;C.gigas:NOAEC=10mg/L2No LD50 YANOVA,Duncan'smulitplerangetest,Chi"squareanalysisLD50=7.5lb/Aat100gal/A;noreducedgrowthorabnormalsurvivorsatlessthantheLD503No 4"dayand7dayLD50s Y Probitanalysis4"dayLD50=0.006mga.i./cm2;7"dayLD50=0.00369mga.i./cm22Bayer CropScienceReport Number US 0202Page 70 of 85BromoxynilLiteratureReviewJuly11,20111AB AC AD AE AF AG**Chemical analysis?** **Statistics Results Score (1** **" 3)** **Paper reports** **a calculated** **endpoint?**3940414243NoInterpretedLC100Y Tukey'sHSDtest 48"hourLC100=0.00393mga.i./cm2 3No No N NotreportedBromoxyniltreatmentreducedgrainyieldwhenappliedtostage1wheat,butincreasedyieldwhenappliedtoolderstages.Slightphytotoxicitywasobservedatstages2and4.3No%visibleinjury,%survival,yield,no.plants/m2NANOVA,GENSTAT5,LSD(leastsignificantdifference)calculated%injuryrangedfrom8"83%,dependingonspeciesandtimeaftertreatment;%survival15"68%;reducedyield3NoNOAEL(nutsedgesurvival,turfgrassinjuryscale)Y NonereportedNOAEL=0.25lbsa.i./acrefornutsedgesurvival;noturgrassinjuryateitherrate(NOAEL=0.5lbsa.i./acreforturfgrassinjury)2No No N ANOVAExposurealteredchlorphyllfluorescenceinbarleyforonlythefirst24hafterapplication;soybeanchlorophyllfluorescencewasinhibited120 h34344454647at120hNotreportedNone(>or<50%growthinhibition)Y NonereportedGrowthinhibition=<50%at1ppm;<50%at10ppm3NotreportedNone(>or<50%growthinhibition)Y NonereportedGrowthinhibition=<50%at1ppmforallspeciestested;Growthinhibition=>50%at10ppmforoats(rootsandshoots)butnototherspecies3NotreportedEC50(photosynthesisinhibition)Y LinearregressionChlorella:EC50=4.4064mg/LScenedesmus:EC50=53.4359mg/L2No EC50(growth) Y Linearregressionanalysis 96"hourEC50=89.1256mg/L 2Bayer CropScienceReport Number US 0202Page 71 of 85BromoxynilLiteratureReviewJuly11,20111AB AC AD AE AF AG**Chemical analysis?** **Statistics Results Score (1** **" 3)** **Paper reports** **a calculated** **endpoint?**484950Notreported EC50(growth) Y Linearregressionanalysis EC50=3.7mg/L3Notreported EC50(growth) Y Linearregressionanalysis EC50=4.2286mg/L(growthinhibition) 3NotreportedNOAEC,LOAEC,EC50(growth)Y LinearregressionanalysisC.vulgaris:EC50=5.72mg/L;LOAEC=1mg/L;NOAEC=0.5mg/LC.pyrenoidosa:EC50=324.55mg/L;LOAEC=100mg/L;NOAEC=50mg/LS.obliquus:EC50=7.54mg/L;LOAEC=2mg/L;NOAEC=1mg/LS.quadricauda:EC50=5.82mg/L;LOAEC=0.5mg/L;NOAEC=0.2mg/LP.subcapitata:EC50=4.43mg/L;LOAEC=1mg/L; NOAEC=0.5 mg/L251525354No No N LSDOnlyfourspecieswereseriouslyaffectedbyioxynil+bromoxynil:Carduusacanthoides,Coniummaculatum,Veronicapersica,Alliariapeteolata.Authorsconcludedthatthisherbicidewasoflimitedtoxicitytoedge"of"fieldplantspecies3NoInterpretedNOAEL(growth,yield,proteincontent)YANOVAforgrowth,Fisher'sProtectedLSDforgrainyieldNOAEL=0.55kg/haforgrowthandyield,andproteincontent2No No N Notreported N/A3No No N Duncan'smultiplerangetestHalf"lifeofbeepopulationwasincreasedovercontrols(17.5days)at10ppm(34.5days)and100ppm(33.8days),butreducedat1000ppm(2.9days)3Bayer CropScienceReport Number US 0202Page 72 of 85BromoxynilLiteratureReviewJuly11,20111AB AC AD AE AF AG**Chemical analysis?** **Statistics Results Score (1** **" 3)** **Paper reports** **a calculated** **endpoint?**55565758No No N LeastSignificantDifferenceBromoxynilseedtreatmentdidnotpreventslugfeedingonseedlings;perennialryegrassgerminationwassignificantlyreducedbyalltreatments3NoInterpretedNOAEL(topgrowthkilled),observationsat2and28daysYANOVA,Duncan'smultiplerangetest83"100%topgrowthkillforrape,noeffectonotherspecies(NOAEL=0.35kg/ha)2NoInterpretedNOAEL(visibleinjury,yield)Y FisherProtectedLSDNOAEL=0.3kga.i./haforgrainyield;7"20%injury2NoInterpretedNOAEL(soybeaninjury)Y LSD100%controlofjimsonweedat0.140kg/haoverentireplant,withlessercontrolatotherapplications;NOAEL0.14kg/haforsoybeaninjury2Notreported InterpretedNOAEC(growth) Y t"testsBothspecies:nostatisticallysignificantdifferences in growth at 0 280 mg/L2596061differencesingrowthat0.280mg/LNotreported InterpretedNOAEC(growth) Y t"testsAllspecies:Nostatisticallysignificantdifferenceingrowthat0.280mg/L2Notreported InterpretedNOAEC(growth) Y t"testsOnlyNitzschiasp.exhibitedstatisticallysignificantdifferenceingrowthat0.280mg/L(40%enhancement!)2Bayer CropScienceReport Number US 0202Page 73 of 85BromoxynilLiteratureReviewJuly11,20111AB AC AD AE AF AG**Chemical analysis?** **Statistics Results Score (1** **" 3)** **Paper reports** **a calculated** **endpoint?**626364656667NotreportedInterpretedNOAEC(growthinhibition)Y t"testsNostatisticsignificantdifferenceingrowthat0.280mg/L2No No NLSDforfieldexperiment100%injury(death)forbothgreenhouseandfieldexperiments3N/A No N N/A Toxicitydatafromdatabase 3NoNOAEC(mortality,eggproduction,hatch)Y ANOVAandChiSquaretestNOAEC=~0.6μL/cm2(mortality/eggproduction/hatch)or830ppm,whenbromoxynilwastestedalone(effectsseenwithbromoxynilmixtures)2NoIC50/EC50(injury,yield,weight)YLog"logisticdose"responsemodel,linearregression,SpearmanranktestI50s(EC50s)forreducedherbageyield(dry)0.65"1.22proportionofrecommendedherbiciderate(0.28ga.i./ha),dependingonsprayingtime,leafstage,andcultivar2NoED50s(doserequiredtoreduce dry weight by 50%)Y ANOVAp=0.05ED50=2400g/haforwheat,22g/haforfiddleneck16768697071reducedryweightby50%)fiddleneckNo EC50(growth) YLinearregressionanalysis,Dunnett'stest,Student'sttestMicroplate:EC50=3.4mg/LFlask:EC50=2.9mg/L2No No N NotreportedBromoxyniltreatmentreducedCO2fixationto5.11μMCO2/mgchlorophyllxhour(control=10.8μMCO2/mgchlorophyllxhour)3NoNumberanddrymassofnodulesY Averages,notspecified Noeffectonnoduleformation 3Notreported 96"hourEC50 Y Notreported 96"hourEC50=8mg/L3Bayer CropScienceReport Number US 0202Page 74 of 85BromoxynilLiteratureReviewJuly11,20111AB AC AD AE AF AG**Chemical analysis?** **Statistics Results Score (1** **" 3)** **Paper reports** **a calculated** **endpoint?**7273747576Notreported 48"hourLC50 Y Notreported 48"hourLC50=12.5mg/L 3Notreported 96"hourLC50 Y Notreported 96"hourLC50=0.15mg/L 3Yes EC50(growth) YStraight"linegraphicalinterpretationS.costatum:EC50=0.86to2.5mg/LdependingonsaltwatertypeT.pseudonana:EC50=1.2"5mg/LdependingonsaltwatertypeChlorellasp.:EC50=3.1"10mg/Ldependingonsaltwatertype3NoInterpretedLOAEL:Visibleinjury,andreducedyieldY BayesLSDp=0.050.3kg/hawasphytotoxic,0.1kg/haproducedlowlevelsofinjuryinsoybeans2NoInterpretedNOAEL(weightoftop"growth)fromfieldstudy YLSDp=0.05,Duncan'smultiplerangetestField:soybeanNOAEL=0.6kg/ha(significanteffectsonweedspecies);LOAEL=1.1kg/haforsoybeangrowthingreenhouseexperiment 2(field),3(greenhouse)Bayer CropScienceReport Number US 0202Page 75 of 85BromoxynilLiteratureReviewJuly11,201112345AH**Comments**NOAELsandLOAELsinterpretedfromdataintablesfor1and2yroldtrees,20to120DAT;statisticalmethodreportedNOAELsandLOAELsinterpretedfromdataintables;statisticalmethodreportedLOAELsinterpretedfromdataintables;statisticalmethodreportedLOAELsinterpretedfromdataintable,butonlyoneconcentrationwastested(noboundingofexposures/effectrates)Lists LOAELs/EC50s for the various species in an678ListsLOAELs/EC50sforthevariousspeciesinanappendix;analyticalverificationofstocksolutionconcentrationsAnalyticalverificationoftestconcentrationsperformedSmallCIsforEC50valuesindicateappropriatetestconcentrationsused(althoughnotreported)Bayer CropScienceReport Number US 0202Page 76 of 85BromoxynilLiteratureReviewJuly11,20111AH**Comments**91011ExperimentsperformedwithbromoxynilformulationBuctrilNOAECinterpretedfromtable.Nosignificanteffectsathighestconcentrationtested.Nocalculatedendpoint;testorganismswereexposedtoamixtureofherbicides1112131415LOAELinterpretedfromdataintable;statisticalmethodnotreportedNOAECsinterpretedfromtable;graphicalinterpretationreportedatstatisticalmethodinpaperOnlyoneapplicationratetested(noboundingofexposures)QSARstudy(datafromMaetal2002,2006)Bayer CropScienceReport Number US 0202Page 77 of 85BromoxynilLiteratureReviewJuly11,20111AH**Comments**16171819Nocalculatedendpoint;Unclearifcontroleggswereinjected;locationofexperimentnotreportedNocalculatedendpoint;improperendpoint(slugfeedingrate);bromoxyniltoxicitytestedasapartofamixtureImproperendpoint(slugfeedingrate)NocalculatedendpointpresentedPertinent data from Fairchild et al 199720212223PertinentdatafromFairchildetal1997Testconcentrationsnotreported,butacceptableCIreportedTestconcentrationsnotreported,wideCIreportedforL.minorendpointsNOAECinterpretedfromtable;toxicityofbromoxynilwasdeterminedatdifferentpHs(mosttoxicatpH5)Bayer CropScienceReport Number US 0202Page 78 of 85BromoxynilLiteratureReviewJuly11,20111AH**Comments**242526Analyticalverificationoftestconcentrations,butcontrolsnotappropriatelyhandled(notplacedinwindtunnelsasweretreatmentplants)Analyticalverificationoftestconcentrations,butcontrolsnotappropriatelyhandled(notplacedinwindtunnelsasweretreatmentplants)Analyticalverificationoftestconcentrations,butcontrolsnotappropriatelyhandled(notplacedinwindtunnelsasweretreatmentplants)UnboundedNOAELinterpretedfrompresented272829p pdata(onlyoneapplicationratetested)UnboundedNOAELinterpretedfrompresenteddata(onlyoneapplicationratetested)ExperimentconductedacccordingtoOECDmethodology;NOAECvalueinterpretedfromgraphBayer CropScienceReport Number US 0202Page 79 of 85BromoxynilLiteratureReviewJuly11,20111AH**Comments**30313233NOAELvaluesinterpretedfromgraphsDailyanalyticalverificationoftestconcentrationsperformed,methodologyapproximatesOECDstandardNOAECandLOAECvaluesinterpretedfromtableBromoxynilformulationtestedalsocontainedMCPA3435363738Exposurewastoherbicidemixturecontaining0.5g/habromoxynilSimilartoshelldepositiontestChronictestswithalgaeperformedaspartofaboveexperimentwithoysterlarvaeConcentrationsnotreported;bromoxyniltestedaspartofmixtureConcentrationsnotreported,butCIsmallenoughtoindicateacceptabilityoftestconcentrationsBayer CropScienceReport Number US 0202Page 80 of 85BromoxynilLiteratureReviewJuly11,20111AH**Comments**3940414243UnboundedLC100interpretedfromdata(lowesttestedconcentration)NoendpointreportedOnlyoneapplicationratetested(noboundingofexposures/effectrates)Onlyoneconcentrationtestedandnoendpointswerereported.4344454647Resultsreportedqualitatively,cannotinterpretendpointfromdatapresentedResultsreportedqualitatively,cannotinterpretendpointfromdatapresentedStatisticalmethodsnotfullyreportedBayer CropScienceReport Number US 0202Page 81 of 85BromoxynilLiteratureReviewJuly11,20111AH**Comments**484950NeithertestconcentrationsorendpointCIreportedNeithertestconcentrationsorendpointCIreported51525354Bromoxyniltestedasapartofabromoxynil+ioxynilmixture;noendpointreportedOnlyoneapplicationratetested(noboundingofexposures);unboundedNOAELinterpretedfromtableReviewarticleImproperendpoint:daysuntil50%oftreatedbeesdieBayer CropScienceReport Number US 0202Page 82 of 85BromoxynilLiteratureReviewJuly11,20111AH**Comments**55565758Improperendpoint(slugfeedingrate);problemwithcontrolappropriatenessOnlyoneapplicationratetested(noboundingofexposures);unboundedNOAELinterpretedNOAELinterpretedfromdata/figuresMeetsmostcriteriabutcontrolratingsystemdifficulttotranslateto%effect;insufficientreplication?UnboundedNOAECinterpretedfromtable596061UnboundedNOAECinterpretedfromtableUnboundedNOAECinterpretedfromtableBayer CropScienceReport Number US 0202Page 83 of 85BromoxynilLiteratureReviewJuly11,20111AH**Comments**626364656667UnboundedNOAECinterpretedfromtableOnlyoneapplicationratetested(noboundingofexposures/effectrates)Nooriginaldata;QSARstudyUnboundedNOAECinterpretedfromtable;effectsonbeetleobservedonlywhenbromoxyniltestedaspartofmixtureEC50sinterpretedfromgraphsandI50s,rangingfrom0.18"0.34ga.i./haCalculatesED50s;includesstatistics,detailsoncontrols6768697071controlsConcentrationsnotreported,butCIssmallenoughtoindicateacceptabilityoftestconcentrationsNocalculatedendpoint;appropriatenessofcontrolsunclearUnclearbromoxyniltestconcentrationPapernottheprimarysourceofdata;speciesnotidentified;concentrationsandcontrolsnotspecifiedBayer CropScienceReport Number US 0202Page 84 of 85BromoxynilLiteratureReviewJuly11,20111AH**Comments**7273747576Papernottheprimarysourceofdata;concentrationsandcontrolsnotspecifiedPapernottheprimarysourceofdata;speciesnotidentified;concentrationsandcontrolsnotspecifiedNeithertestconcentrationsorendpointCIreportedUnboundedLOAELinterpretedfromtableEachexperimenttestedonlyoneapplicationrate,butonlyfielddataprovidedanunboundedNOAELBayer CropScienceReport Number US 0202Page 85 of 85