# Patent

## Title
2,3-fluorinated glycosides as neuraminidase inhibitors and their use as anti-virals

## Abstract

Compounds having a structure of Formula I and compositions comprising these compounds are provided. Uses of such compounds and compositions are provided for treatment or prophylaxis of viral infection. In particular, compounds and compositions may be for use in the treatment or prophylaxis of viral influenza.

## Background
CROSS REFERENCE TO RELATED APPLICATIONS
This application is a continuation of U.S. application Ser. No. 13/382,284filed on Mar. 26, 2012, which is a National Stage Entry of PCT/CA10/01063 filed on Jul. 15, 2010, which claims the benefit of U.S. Provisional Patent Application Ser. No. 61/213,786 entitled “NEURAMIDINASE INHIBITORS: COMPOSITIONS AND METHOD OF USE FOR THE TREATMENT OF INFLUENZA” filed on Jul. 15, 2009, the disclosures of each of which are incorporated herein by reference in their entirety.


TECHNICAL FIELD
This invention relates to therapeutics, their uses and methods for the treatment or prophylaxis of viral infection. In particular the invention relates to compounds, compositions, therapies, and methods of treatment for viral infections such as influenza.
BACKGROUND
Infection and invasion by influenza viruses requires the intermediacy of sialic acid residues on the surface of the host cell. Sialic acid and neuraminic acid are used interchangeably. Similarly, sialidase and neuraminidase are used interchangeably. Initial attachment of the virus to the host cell occurs via the binding of the virus to these sialic acids (charged, 9-carbon sugars) through the hemagglutinin protein of the virus. Once inside the cell the virus replicates by taking advantage of the host cellular machinery. However, in order to remain optimally infective, the virus has evolved a neuraminidase that cuts off the sialic acid from the host cell surface to assist the virus in escaping the host cell to infect other cells. Failure to cut off the sialic acid from the host cell surface, results in retention of virus through attachment to the host cell.
The GH33 family of neuraminidases contains all the sialidases except the viral enzymes (GH34 family). The GH33 and GH34 families are distinct structurally and by sequence (See Cantarel BL. et al. (2009); and Henrissat B. and Davies G.J (1997) for background on Family classifications). Previous work has demonstrated that 2,3-difluorosialic acids are effective inhibitors of GH33 neuraminidases and that GH33 neuraminidases proceed through a covalent intermediate (see for example, Watts, A. et al. (2003); Amaya, M. F. et al. (2004); Watts, A. G. and Withers, S. G. (2004); Watts, A. G. et al. (2006); Newstead, S. et al. (2008); Damager, I. et al. (2008); and Buchini, S. et al. (2008)).
The most probable mechanism for the GH34 sialidase (i.e. viral sialidases) reported in the literature is one involving an ion-pair intermediate (von Itzstein M. (2007)).
A number of compounds are known to inhibit neuraminidases. Some well known neuraminidase inhibitors are alkene containing sialic acid analogues (for example: Laninamivir CAS #203120-17-6; Oseltamivir (Tamiflu) CAS #204255-11-8; and Zanamivir (Relenza) CAS #139110-80-8; see also U.S. Pat. No. 5,360,817; and Ikeda et al. Bioorganic & Medicinal Chemistry (2006) 14:7893-7897). Fluorinated sugar derivatives with (reactive) fluoride leaving groups have been shown to be inhibitors of a range of “retaining” glycosidases and function via formation of particularly stable glycosyl-enzyme intermediates (for example, Hagiwara et al. (1994); and Buchini et al. (2008)). These reagents are quite specific with respect to their target enzymes, have been shown to be highly bio-available, and even capable of crossing the blood-brain barrier. Such inhibitors are mechanism-based in their action, making the development of resistance by viruses difficult, whereby any mutations in the viral enzyme that reduce the inhibition must necessarily reduce the efficiency of the enzyme on the natural substrate, sialic acid and therefore less likely to be tolerated.
SUMMARY
This invention is based in part on the fortuitous discovery that compounds having a covalent intermediate, as described herein, modulate neuraminidase. Specifically, compounds identified herein, show inhibition of neuraminidase, which may be useful for the treatment or prophylaxis of viral infection. In particular, the treatment or prophylaxis of influenza.
The compounds described herein may be used for in vivo or in vitro research uses (i.e. non-clinical) to investigate the mechanisms of neuraminidase inhibition. Furthermore, these compounds may be used individually or as part of a kit for in vivo or in vitro research to investigate neuraminidase inhibition using recombinant proteins, viral strains, cells maintained in culture, and/or animal models. Alternatively, the compounds described herein may be combined with commercial packaging and/or instructions for use.
This invention is also based in part on the discovery that the compounds described herein, may also be used to modulate neuraminidase activity either in vivo or in vitro for both research and therapeutic uses. The compounds may be used in an effective amount so that neuraminidase activity may be modulated. The neuraminidase may be viral. The neuraminidase may be an influenza neuraminidase. In particular, the compounds may be used to inhibit neuraminidase activity. The compounds modulatory activity may be used in either an in vivo or an in vitro model for the study of viral infection. For example, influenza infection. Furthermore, the compounds modulatory activity may be used for the treatment or prophylaxis of viral infection. The viral infection may be influenza.
Furthermore, this invention is based in part on the appreciation that 3-fluoro-sialic acids compounds may be GH34 sialidase inhibitors provided that the compounds have a sufficient leaving group at carbon 2 (position Z in Formula I) in addition to the appropriate stereochemistry, as described herein. Compounds identified herein, show inhibition of neuraminidase, which may be useful for the treatment or prophylaxis of viral infection. In particular the treatment or prophylaxis of influenza.
In accordance with one embodiment, there are provided compounds having a structure of Formula I:




    
    
        wherein
        
            T is C(O)NH2, COOH or COOR1,
            
                wherein R1 is a C1-20 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group,
                
                    wherein the optional substituent is selected from one or more of the group including of: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2, and
                    wherein zero to ten backbone carbons of the optionally substituted alkyl group may be optionally and independently substituted with O, N or S;
                
                
            
            
            Z is F, Cl, Br, or OSO2R2,
            
                wherein R2 is a C1-20 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group,
                
                    wherein the optional substituent is selected from one or more of the group including of: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2, and
                    wherein zero to ten backbone carbons of the optionally substituted alkyl group may be optionally and independently substituted with O, N or S;
                
                
            
            
            A is selected from the group including of: H, F, Cl, Br, OH, CN, OR3, NO2, SO2R3, SR3 and COR3,
            
                wherein R3 is a C1-20 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group,
                
                    wherein the optional substituent is selected from one or more of the group including of oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2, and
                    wherein zero to ten backbone carbons of the optionally substituted alkyl group may be optionally and independently substituted with O, N or S;
                
                
            
            
            D may be selected from the group including of: H, F, Cl, Br, OH, CN, OR4, NO2, SO2R4, SR4 and COR4, provided A and D may be not both H, and
            
                wherein R4 may be a C1-20 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group,
                
                    wherein the optional substituent may be selected from one or more of the group including of: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2, and
                    wherein zero to ten backbone carbons of the optionally substituted alkyl group may be optionally and independently substituted with O, N or S;
                
                
            
            
            collectively, A and D, optionally form an oxo group;
            X may be selected from the group including of: N3, NH2, NHR5, NHCH3, NHCH2CH3, NHC(NH)NH2, NHC(NH)NHR5, NR5R6, and NHC(NH)N(R5)R6,
            
                wherein R5 and R6 may be independently C6H5, CH2C6H5 or a C1-8 alkyl group;
            
            
            E may be selected from the group including of NH2, NHC(O)CH3, OR7, NHR7 and N(R7)(R8),
            
                wherein R7 and R8 may be independently a C1-20 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group,
                
                    wherein the optional substituent may be selected from one or more of the group including of: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2, and
                    wherein zero to ten backbone carbons of the optionally substituted alkyl group may be optionally and independently substituted with O, N or S;
                
                
            
            
            Q may be selected from the group including of: CH2OH, CH2R9, CH(R9)(R10),) C(R9)(R10)(R11),
        
        
    
    






    
    
        
        
            
            
                wherein
                
                    R9, R10 and R11 may be independently CH3 or CH2CH3, and
                    each of J and G may be independently selected from the group: H, OH, OAc, OC(O)CH3, F, Cl, Br, NO2, CN, OR12, SO2R12, COR12 and SR12,
                     wherein R12 may be CH3, CH2CH3 or CH2CH2CH3, and
                    M may be H, OH, OAc, OC(O)CH3, NH2, F or Cl, and
                    L may be H, OH, OAc, OC(O)R13 or NH2,
                     wherein R13 may be a C1-20 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group,
                     wherein the optional substituent may be selected from one or more of the group: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2, and
                     wherein zero to ten backbone carbons of the optionally substituted alkyl group may be optionally and independently substituted with O, N or S.
                
                
            
            
        
        
    
    


In accordance with a further embodiment, there are provided compounds having a structure of formula I:




    
    
        wherein
        
            T may be C(O)NH2, COOH or COOR1,
            
                wherein R1 may be a C1-20 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group,
                
                    wherein the optional substituent may be selected from one or more of the group including of: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2, and
                    wherein zero to ten backbone carbons of the optionally substituted alkyl group may be optionally and independently substituted with O, N or S;
                
                
            
            
            Z may be F, Cl, Br, or OSO2R2,
            
                wherein R2 may be a C1-20 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group,
                
                    wherein the optional substituent may be selected from one or more of the group including of: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2, and
                    wherein zero to ten backbone carbons of the optionally substituted alkyl group may be optionally and independently substituted with O, N or S;
                
                
            
            
            A may be F or Cl;
            D may be H;
            X may be selected from the group including of: NH2, NHCH3, NHCH2CH3, NHCH2CH2CH3, NHCH2CH2CH2CH3, and NHC(NH)NH2;
            E may be NH2 or NHC(O)CH3;
            Q may be selected from the group including of:
        
        
    
    






    
    
        
        
            
            
                wherein
                
                    each of J and G may be independently selected from the group: H, OH, OAc, OC(O)CH3, F, Cl, Br, NO2, CN, OR12, SO2R12, COR12 and SR12,
                     wherein R12 may be CH3, CH2CH3 or CH2CH2CH3, and
                    M may be H, OH, OAc, OC(O)CH3, NH2, F or Cl, and
                    L may be H, OH, OAc, OC(O)R13 or NH2,
                     wherein R13 may be a C1-10 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group,
                     wherein the optional substituent may be selected from one or more of the group: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2, and
                     wherein zero to ten backbone carbons of the optionally substituted alkyl group may be optionally and independently substituted with O, N or S.
                
                
            
            
        
        
    
    


In accordance with a further embodiment, there are provided compounds having a structure of formula I:




    
    
        wherein
        
            T may be C(O)NH2, COOH or COOR1,
            
                wherein R1 may be a C1-20 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group,
                
                    wherein the optional substituent may be selected from one or more of the group including of: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2, and
                    wherein zero to ten backbone carbons of the optionally substituted alkyl group may be optionally and independently substituted with O, N or S;
                
                
            
            
            Z may be F or Cl;
            A may be F or Cl;
            D may be H;
            X may be selected from the group including of: NH2, NHCH3, NHCH2CH3, NHCH2CH2CH3, NHCH2CH2CH2CH3, and NHC(NH)NH2;
            E may be NH2 or NHC(O)CH3;
            Q may be selected from the group including of:
        
        
    
    






    
    
        
        
            
            
                wherein
                
                    each of J and G may be independently selected from the group: H, OH, OAc, OC(O)CH3, F, Cl, Br, NO2, CN,
                    M may be H, OH, OAc; and
                    L may be H, OH, OAc.
                
                
            
            
        
        
    
    


In accordance with a further embodiment, there are provided compounds as described herein for modulating viral neuraminidase activity. The viral neuraminidase may be a GH34 neuraminidase. The modulating of viral neuraminidase activity may be for the treatment of influenza in an animal. The animal may be a mammal. The animal may be a human.
In accordance with a further embodiment, there are provided compounds as described herein for use in the preparation of a medicament for modulating viral neuraminidase activity. The viral neuraminidase may be a GH34 neuraminidase. The modulating of viral neuraminidase activity may be for the treatment of influenza in an animal. The animal may be a mammal. The animal may be a human.
In accordance with a further embodiment, there are provided compounds as described herein for modulating viral neuraminidase activity. The viral neuraminidase may be a GH34 neuraminidase. The modulating of viral neuraminidase activity may be for the treatment of influenza in an animal. The animal may be a mammal. The animal may be a human.
In accordance with a further embodiment, there are provided pharmaceutical compositions which may include one or more compounds as described herein and a pharmaceutically acceptable excipient. The viral neuraminidase may be a GH34 neuraminidase. The modulating of viral neuraminidase activity may be for the treatment of influenza in an animal. The animal may be a mammal. The animal may be a human.
In accordance with a further embodiment, there are provided compounds or pharmaceutically acceptable salts thereof as described herein for modulating viral neuraminidase activity. The viral neuraminidase may be a GH34 neuraminidase. The modulating of viral neuraminidase activity may be for the treatment of influenza in an animal. The animal may be a mammal. The animal may be a human.
In accordance with a further embodiment, there is provided a method of modulating viral neuraminidase activity with one or more compounds described herein or a pharmaceutically acceptable salt thereof. The viral neuraminidase may be a GH34 neuraminidase. The modulating of viral neuraminidase activity may be for the treatment of influenza in an animal. The animal may be a mammal. The animal may be a human.
In accordance with a further embodiment, there is provided a commercial package which may contain one or more compounds described herein or a pharmaceutically acceptable salt thereof or a pharmaceutical composition thereof. The commercial package may optionally contain instructions for the use of the compounds or pharmaceutically acceptable salt thereof or pharmaceutical composition thereof in the treatment of influenza.
T may be C(O)NH2, COOH or COOR1, wherein R1 may be a C1-20 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group, wherein the optional substituent may be selected from one or more of the group including of: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2. Alternatively, T may be C(O)OCH3, C(O)OCH2CH3, C(O)OCH2CH2CH3, C(O)OCH2CH2CH2CH3, C(O)OCH2CH2CH2CH2CH3, C(O)OCH2CH2CH2CH2CH2CH3, C(O)OCH2CH2CH2CH2CH2CH2CH3, C(O)OCH2CH2CH2CH2CH2CH2CH2CH3, or COOH. Alternatively, T may be C(O)OCH3, C(O)OCH2CH3, C(O)OCH2CH2CH3, C(O)OCH2CH2CH2CH3, C(O)OCH2CH2CH2CH2CH3, or COOH.
A may be selected from the group including of: F, Cl, Br, OH, CN, OR3, NO2, and COR3, wherein R3 may be a C1-20 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group, wherein the optional substituent may be selected from one or more of the group including of: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2, and wherein zero to ten backbone carbons of the optionally substituted alkyl group may be optionally and independently substituted with O, N or S. Alternatively, A may be selected from the group including of: F, Cl, Br, OH, CN, OR3, and NO2, wherein R3 may be a C1-20 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group, wherein the optional substituent may be selected from one or more of the group including of: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2, and wherein zero to ten backbone carbons of the optionally substituted alkyl group may be optionally and independently substituted with O, N or S. Alternatively, A may be selected from the group including of: F, Cl, Br, OH, CN, and NO2. Alternatively, A may be selected from the group including of F, Cl, Br, OR3, and NO2, wherein R3 may be a C1-20 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group, wherein the optional substituent may be selected from one or more of the group including of: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2. Alternatively, A may be selected from the group including of: F, Cl, and OR3, wherein R3 may be a C1-20 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group, wherein the optional substituent may be selected from one or more of the group including of: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2. Alternatively, A may be F, Cl. Alternatively, A may be F.
D may be selected from the group including of: H, F, Cl, Br, OH, CN, OR4, NO2, and COR4, provided A and D may be not both H, and wherein R4 may be a C1-10 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group, wherein the optional substituent may be selected from one or more of the group including of: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2, and wherein zero to ten backbone carbons of the optionally substituted alkyl group may be optionally and independently substituted with O, N or S. Alternatively, D may be selected from the group including of: H, F, Cl, Br, OH, CN, OR4, NO2, and COR4, provided A and D may be not both H, and wherein R4 may be a C1-10 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group, wherein the optional substituent may be selected from one or more of the group including of: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2. Alternatively, D may be selected from the group including of: H, F, Cl, Br, OH, CN, and NO2, provided A and D may be not both H. Alternatively, D may be selected from the group including of: H, F, Cl, Br, OH and NO2, provided A and D may be not both H. Alternatively, D may be selected from the group including of: H, F, Cl, Br, and OH, provided A and D may be not both H. Alternatively, D may be selected from the group including of: H, F, Cl, and Br, provided A and D may be not both H. Alternatively, D may be selected from the group including of: H, F, and Cl, provided A and D may be not both H. Alternatively, D may be selected from the group including of: H, F, and Cl, provided A and D may be not both H. Alternatively, D may be F or Cl. Alternatively, D may be H, or F, provided A and D may be not both H. Alternatively, D may be F. Alternatively, D may be H, provided A and D may be not both H.
X may be selected from the group including of: NH2, NHR5, NHCH3, NHCH2CH3, NHC(NH)NH2, NHC(NH)NHR5, and NR5R6, wherein R5 and R6 may be independently C6H5, CH2C6H5 or a C1-8 alkyl group. Alternatively, X may be selected from the group including of: NH2, NHR5, NHCH3, NHCH2CH3, NHC(NH)NH2, and NHC(NH)NHR5, wherein R5 may be C6H5, CH2C6H5 or a C1-8 alkyl group. Alternatively, X may be selected from the group including of: NH2, NHR5, NHCH3, NHCH2CH3, and NHC(NH)NH2, wherein R5 may be C6H5, CH2C6H5 or a C1-8 alkyl group. Alternatively, X may be selected from the group including of NH2, NHR5, NHCH3, NHCH2CH3, and NHC(NH)NH2, wherein R5 may be C6H5, CH2C6H5 or a C1-8 alkyl group. Alternatively, X may be selected from the group including of: NH2, NHR5, NHCH3, NHCH2CH3, and NHC(NH)NH2, wherein R5 may be a C1-8 alkyl group. Alternatively, X may be selected from the group including of: NH2, NHCH3, and NHC(NH)NH2. Alternatively, X may be selected from the group including of: NH2, NHCH2CH3, and NHC(NH)NH2. Alternatively, X may be NH2 or NHC(NH)NH2.
E may be selected from the group including of: NH2, NHC(O)CH3, OR7, NHR7, wherein R7 may be independently a C1-10 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group, wherein the optional substituent may be selected from one or more of the group including of: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2, and wherein zero to ten backbone carbons of the optionally substituted alkyl group may be optionally and independently substituted with O, N or S. Alternatively, E may be selected from the group including of: NH2, NHC(O)CH3, OR7, and NHR7, wherein R7 may be independently a C1-10 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group, wherein the optional substituent may be selected from one or more of the group including of: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2. Alternatively, E may be selected from the group including of: NH2, NHC(O)CH3, and OR7, wherein R7 may be independently a C1-10 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group, wherein the optional substituent may be selected from one or more of the group including of: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2. Alternatively, E may be NH2 or NHC(O)CH3. Alternatively, E may be NHC(O)CH3.
Q may be selected from the group including of: CH2R9, CH(R9)(R10), C(R9)(R10)(R11),




wherein R9, R10 and R11 may be independently CH3 or CH2CH3, and each of J and G may be independently selected from the group: H, OH, OAc, OC(O)CH3, F, Cl, Br, NO2, CN, OR12, SO2R12, COR12 and SR12, wherein R12 may be CH3, CH2CH3 or CH2CH2CH3, and M may be H, OH, OAc, OC(O)CH3, NH2, F or Cl, and L may be H, OH, OAc, OC(O)R13 or NH2, wherein R13 may be a C1-20 linear, branched or cyclic, saturated or unsaturated, optionally substituted alkyl group, and wherein the optional substituent may be selected from one or more of the group: oxo, OH, F, Cl, Br, I, NH2, CN, SH, SO3H and NO2. Alternatively, Q may be selected from the group including of:





each of J and G may be independently selected from the group: H, OH, OAc, OC(O)CH3, F, Cl, Br, NO2, CN, M may be H, OH, OAc, OC(O)CH3, NH2, F or Cl, and L may be H, OH, OAc, OC(O)R13 or NH2. Alternatively, Q may be selected from the group including of:





each of J and G may be independently selected from the group: H, OH, OAc, M may be H, OH, or OAc, and L may be H, OH, or OAc. Alternatively, Q may be:





each of J and G may be independently selected from the group: H, OH, OAc, M may be H, OH, or OAc, and L may be H, OH, or OAc. Alternatively, Q may be:





each of J and G may be independently selected from the group: OH, OAc, M may be OH, or OAc, and L may be OH, or OAc.

In accordance with a further embodiment, there is provided a method of preparing compound 2:
the method including: reacting a compound SAN3:




with Selectfluor in the presence of MeNO2/H2O for at least 4 days to form compound 1:





and reacting compound 1 with diethylaminosulfur trifluoride (DAST), CH2CL2 at between −30° C.-0° C.

The method may further include: mixing compound 2 with NaOMe and MeOH; then mixing with Pd/C, H2, and MeOH; and then mixing with LiOH, H2O, and MeOH to form compound 4:




In accordance with a further embodiment, there is provided a method of preparing compound 12, the method may include mixing compound 2:




with NaOMe and MeOH; then mixing with AcOH until neutral; then mixing with PMe3, H2O, and MeOH; and then reacting with compound VII:





in Et3N, MeOH, and DMF to produce compound VIII:





then reacting compound VIII with LiOH, H2O, and THF; and then reacting with Pd/C, H2, H2O, and THF.

In accordance with a further embodiment, there are provided compounds which may be selected from one or more of the compounds set out in TABLES 2A and 2B.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows a neuraminidase mechanism.
FIG. 2 shows inactivation of a neuraminidase by 2,3-difluorosialic acid (1).
FIGS. 3A & 3B show a time-dependent inactivation of influenza sialidase (subtype N9) by compound 4, indicated by concentration (3A), and re-plot of pseudo-first order inactivation kinetic constants (ki obs) versus concentration of compound 4 (3B).
FIGS. 4A & 4B show DFSA-4Gu Plasma levels after IV and IN administration.
FIGS. 5A, 5B, & 5C show DFSA-4Gu Trachea levels after IV and IN administration.
FIGS. 6A, 6B, & 6C show DFSA-4Gu Lung levels after IV and IN administration.
FIG. 7 shows a Zanamivir Lung levels after IV and IN administration.
FIG. 8 shows a survival plot of mice infected with HK1 H2N3 Influenza A virus and treated with neuraminidase inhibitors.



DETAILED DESCRIPTION
As used herein, the phrase “Cx-y alkyl” or “Cx-Cy alkyl” is used as it is normally understood to a person of skill in the art and often refers to a chemical entity that has a carbon skeleton or main carbon chain comprising a number from x to y (with all individual integers within the range included, including integers x and y) of carbon atoms. For example a “C1-10 alkyl” is a chemical entity that has 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 carbon atom(s) in its carbon skeleton or main chain.
As used herein, the term “branched” is used as it is normally understood to a person of skill in the art and often refers to a chemical entity that comprises a skeleton or main chain that splits off into more than one contiguous chain. The portions of the skeleton or main chain that split off in more than one direction may be linear, cyclic or any combination thereof. Non-limiting examples of a branched alkyl are tert-butyl and isopropyl.
As used herein, the term “unbranched” is used as it is normally understood to a person of skill in the art and often refers to a chemical entity that comprises a skeleton or main chain that does not split off into more that one contiguous chain. Non-limiting examples of unbranched alkyls are methyl, ethyl, n-propyl, and n-butyl.
As used herein, the term “substituted” is used as it is normally understood to a person of skill in the art and often refers to a chemical entity that has one chemical group replaced with a different chemical group that contains one or more heteroatoms. Unless otherwise specified, a substituted alkyl is an alkyl in which one or more hydrogen atom(s) is/are replaced with one or more atom(s) that is/are not hydrogen(s). For example, chloromethyl is a non-limiting example of a substituted alkyl, more particularly an example of a substituted methyl. Aminoethyl is another non-limiting example of a substituted alkyl, more particularly it is a substituted ethyl. The functional groups described herein may be substituted with, for example, and without limitation, 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 substituents.
As used herein, the term “unsubstituted” is used as it is normally understood to a person of skill in the art and often refers to a chemical entity that is a hydrocarbon and/or does not contain a heteroatom. Non-limiting examples of unsubstituted alkyls include methyl, ethyl, tert-butyl, and pentyl.
As used herein, the term “saturated” when referring to a chemical entity is used as it is normally understood to a person of skill in the art and often refers to a chemical entity that comprises only single bonds. Non-limiting examples of saturated chemical entities include ethane, tert-butyl, and N+H3.
As used herein the term “halogenated” is used as it would normally be understood to a person of skill in the art and refers to a moiety or chemical entity in which a hydrogen atom is replaced with a halogen atom such as chlorine, fluorine, iodine or bromine. For example, a fluorinated side chain refers to a side chain wherein one or more hydrogen atoms is replaced with one or more fluorine atoms.
Non-limiting examples of saturated C1-C10 alkyl may include methyl, ethyl, n-propyl, i-propyl, sec-propyl, n-butyl, i-butyl, sec-butyl, t-butyl, n-pentyl, i-pentyl, sec-pentyl, t-pentyl, n-hexyl, i-hexyl, 1,2-dimethylpropyl, 2-ethylpropyl, 1-methyl-2-ethylpropyl, 1-ethyl-2-methylpropyl, 1,1,2-trimethylpropyl, 1,1,2-triethylpropyl, 1,1-dimethylbutyl, 2,2-dimethylbutyl, 2-ethylbutyl, 1,3-dimethylbutyl, 2-methylpentyl, 3-methylpentyl, sec-hexyl, t-hexyl, n-heptyl, i-heptyl, sec-heptyl, t-heptyl, n-octyl, i-octyl, sec-octyl, t-octyl, n-nonyl, i-nonyl, sec-nonyl, t-nonyl, n-decyl, i-decyl, sec-decyl and t-decyl. Non-limiting examples of C2-C10 alkenyl may include vinyl, allyl, isopropenyl, 1-propene-2-yl, 1-butene-1-yl, 1-butene-2-yl, 1-butene-3-yl, 2-butene-1-yl, 2-butene-2-yl, octenyl and decenyl. Non-limiting examples of C2-C10 alkynyl may include ethynyl, propynyl, butynyl, pentynyl, hexynyl, heptynyl, octynyl, nonynyl and decynyl. Saturated C1-C10 alkyl, C2-C10 alkenyl or C2-C10 alkynyl may be, for example, and without limitation, interrupted by one or more heteroatoms which are independently nitrogen, sulfur or oxygen.
Non-limiting examples of the C6-C10 aryl group may include phenyl (Ph), pentalenyl, indenyl, naphthyl, and azulenyl. The C6-9 aryl-C1-4 alkyl group may be, for example, and without limitation, a C1-4 alkyl group as defined anywhere above having a C6-9 aryl group as defined anywhere above as a substituent. The C6-8 aryl-C24 alkenyl group may be, for example, and without limitation, a C2-4 alkenyl as defined anywhere above having a C6-8 aryl group as defined anywhere above as a substituent. The C6-8 aryl-C2-4 alkynyl group may be, for example, and without limitation, a C2-4 alkynyl group as defined anywhere above having a C6-8 aryl group as defined anywhere above as a substituent. Non-limiting examples of the 4- to 10-membered non-aromatic heterocyclic group containing one or more heteroatoms which are independently nitrogen, sulfur or oxygen may include pyrrolidinyl, pyrrolinyl, piperidinyl, piperazinyl, imidazolinyl, pyrazolidinyl, imidazolydinyl, morpholinyl, tetrahydropyranyl, azetidinyl, oxetanyl, oxathiolanyl, phthalimide and succinimide. Non-limiting examples of the 5- to 10-membered aromatic heterocyclic group containing one or more heteroatoms which are independently nitrogen, sulfur or oxygen may include pyrrolyl, pyridinyl, pyridazinyl, pyrimidinyl, pirazinyl, imidazolyl, thiazolyl and oxazolyl.
The embodiments involving the formulae as described herein include all possible stereochemical alternatives, including those illustrated or described herein.
In some embodiments, the compounds as described herein or acceptable salts thereof above may be used for systemic treatment or prophylaxis of a viral infection. In some embodiments, the compounds as described herein or acceptable salts thereof above may be used in the preparation of a medicament or a composition for systemic treatment or prophylaxis of a viral infection. In some embodiments, methods of systemically treating any of the infections described herein are also provided. Some embodiments, make use of compositions comprising a compound described herein and a pharmaceutically acceptable excipient or carrier. In some embodiments, the viral infection is caused, at least in part, by an influenza virus. Methods of treating any of the indications described herein are also provided. Such methods may include administering a compound as described herein or a composition of a compound as described herein, or an effective amount of a compound as described herein or composition of a compound as described herein to a subject in need thereof.
Compounds as described herein may be in the free form or in the form of a salt thereof. In some embodiments, compounds as described herein may be in the form of a pharmaceutically acceptable salt, which are known in the art (Berge et al., J. Pharm. Sci. 1977, 66, 1). Pharmaceutically acceptable salt as used herein includes, for example, salts that have the desired pharmacological activity of the parent compound (salts which retain the biological effectiveness and/or properties of the parent compound and which are not biologically and/or otherwise undesirable). Compounds as described herein having one or more functional groups capable of forming a salt may be, for example, formed as a pharmaceutically acceptable salt. Compounds containing one or more basic functional groups may be capable of forming a pharmaceutically acceptable salt with, for example, a pharmaceutically acceptable organic or inorganic acid. Pharmaceutically acceptable salts may be derived from, for example, and without limitation, acetic acid, adipic acid, alginic acid, aspartic acid, ascorbic acid, benzoic acid, benzenesulfonic acid, butyric acid, cinnamic acid, citric acid, camphoric acid, camphorsulfonic acid, cyclopentanepropionic acid, diethylacetic acid, digluconic acid, dodecylsulfonic acid, ethanesulfonic acid, formic acid, fumaric acid, glucoheptanoic acid, gluconic acid, glycerophosphoric acid, glycolic acid, hemisulfonic acid, heptanoic acid, hexanoic acid, hydrochloric acid, hydrobromic acid, hydriodic acid, 2-hydroxyethanesulfonic acid, isonicotinic acid, lactic acid, malic acid, maleic acid, malonic acid, mandelic acid, methanesulfonic acid, 2-napthalenesulfonic acid, naphthalenedisulphonic acid, p-toluenesulfonic acid, nicotinic acid, nitric acid, oxalic acid, pamoic acid, pectinic acid, 3-phenylpropionic acid, phosphoric acid, picric acid, pimelic acid, pivalic acid, propionic acid, pyruvic acid, salicylic acid, succinic acid, sulfuric acid, sulfamic acid, tartaric acid, thiocyanic acid or undecanoic acid. Compounds containing one or more acidic functional groups may be capable of forming pharmaceutically acceptable salts with a pharmaceutically acceptable base, for example, and without limitation, inorganic bases based on alkaline metals or alkaline earth metals or organic bases such as primary amine compounds, secondary amine compounds, tertiary amine compounds, quaternary amine compounds, substituted amines, naturally occurring substituted amines, cyclic amines or basic ion-exchange resins. Pharmaceutically acceptable salts may be derived from, for example, and without limitation, a hydroxide, carbonate, or bicarbonate of a pharmaceutically acceptable metal cation such as ammonium, sodium, potassium, lithium, calcium, magnesium, iron, zinc, copper, manganese or aluminum, ammonia, benzathine, meglumine, methylamine, dimethylamine, trimethylamine, ethylamine, diethylamine, triethylamine, isopropylamine, tripropylamine, tributylamine, ethanolamine, diethanolamine, 2-dimethylaminoethanol, 2-diethylaminoethanol, dicyclohexylamine, lysine, arginine, histidine, caffeine, hydrabamine, choline, betaine, ethylenediamine, glucosamine, glucamine, methylglucamine, theobromine, purines, piperazine, piperidine, procaine, N-ethylpiperidine, theobromine, tetramethylammonium compounds, tetraethylammonium compounds, pyridine, N,N-dimethylaniline, N-methylpiperidine, morpholine, N-methylmorpholine, N-ethylmorpholine, dicyclohexylamine, dibenzylamine, N,N-dibenzylphenethylamine, 1-ephenamine, N,N′-dibenzylethylenediamine or polyamine resins. In some embodiments, compounds as described herein may contain both acidic and basic groups and may be in the form of inner salts or zwitterions, for example, and without limitation, betaines. Salts as described herein may be prepared by conventional processes known to a person skilled in the art, for example, and without limitation, by reacting the free form with an organic acid, an inorganic acid, an organic base or an inorganic base, or by anion exchange or cation exchange from other salts. Those skilled in the art will appreciate that preparation of salts may occur in situ during isolation and/or purification of the compounds or preparation of salts may occur by separately reacting an isolated and/or purified compound.
In some embodiments, compounds and all different forms thereof (e.g. free forms, salts, polymorphs, isomeric forms) as described herein may be in the solvent addition form, for example, solvates. Solvates contain either stoichiometric or non-stoichiometric amounts of a solvent in physical association with the compound or salt thereof. The solvent may be, for example, and without limitation, a pharmaceutically acceptable solvent. For example, hydrates are formed when the solvent is water or alcoholates are formed when the solvent is an alcohol.
In some embodiments, compounds and all different forms thereof (e.g. free forms, salts, solvates, isomeric forms) as described herein may include crystalline and/or amorphous forms, for example, polymorphs, pseudopolymorphs, conformational polymorphs, amorphous forms, or a combination thereof. Polymorphs include different crystal packing arrangements of the same elemental composition of a compound. Polymorphs usually have different X-ray diffraction patterns, infrared spectra, melting points, density, hardness, crystal shape, optical and electrical properties, stability and/or solubility. Those skilled in the art will appreciate that various factors including recrystallization solvent, rate of crystallization and storage temperature may cause a single crystal form to dominate.
In some embodiments, compounds and all different forms thereof (e.g. free forms, salts, solvates, polymorphs) as described herein include isomers such as geometrical isomers, optical isomers based on asymmetric carbon, stereoisomers, tautomers, individual enantiomers, individual diastereomers, racemates, diastereomeric mixtures and combinations thereof, and are not limited by the description of the formula illustrated for the sake of convenience.
In some embodiments, pharmaceutical compositions in accordance with this invention may comprise a salt of such a compound, preferably a pharmaceutically or physiologically acceptable salt. Pharmaceutical preparations will typically comprise one or more carriers, excipients or diluents acceptable for the mode of administration of the preparation, be it by injection, inhalation, topical administration, lavage, or other modes suitable for the selected treatment. Suitable carriers, excipients or diluents include those known in the art for use in such modes of administration.
Suitable pharmaceutical compositions may be formulated by means known in the art and their mode of administration and dose determined by the skilled practitioner. For parenteral administration, a compound may be dissolved in sterile water or saline or a pharmaceutically acceptable vehicle used for administration of non-water soluble compounds such as those used for vitamin K. For enteral administration, the compound may be administered in a tablet, capsule or dissolved in liquid form. The tablet or capsule may be enteric coated, or in a formulation for sustained release. Many suitable formulations are known, including, polymeric or protein microparticles encapsulating a compound to be released, ointments, pastes, gels, hydrogels, or solutions which can be used topically or locally to administer a compound. A sustained release patch or implant may be employed to provide release over a prolonged period of time. Many techniques known to one of skill in the art are described in Remington: the Science & Practice of Pharmacy by Alfonso Gennaro, 20th ed., Lippencott Williams & Wilkins, (2000). Formulations for parenteral administration may, for example, contain excipients, polyalkylene glycols such as polyethylene glycol, oils of vegetable origin, or hydrogenated naphthalenes. Biocompatible, biodegradable lactide polymer, lactide/glycolide copolymer, or polyoxyethylene-polyoxypropylene copolymers may be used to control the release of the compounds. Other potentially useful parenteral delivery systems for modulatory compounds include ethylene-vinyl acetate copolymer particles, osmotic pumps, implantable infusion systems, and liposomes. Formulations for inhalation may contain excipients, for example, lactose, or may be aqueous solutions containing, for example, polyoxyethylene-9-lauryl ether, glycocholate and deoxycholate, or may be oily solutions for administration in the form of nasal drops, or as a gel. The formulations may be specifically prepared for intranasal delivery. For example, nasal inhalation.
Compounds or pharmaceutical compositions in accordance with this invention or for use in this invention may be administered by means of a medical device or appliance such as an implant, graft, prosthesis, stent, etc. Also, implants may be devised which are intended to contain and release such compounds or compositions. An example would be an implant made of a polymeric material adapted to release the compound over a period of time.
An “effective amount” of a pharmaceutical composition as described herein includes a therapeutically effective amount or a prophylactically effective amount. A “therapeutically effective amount” refers to an amount effective, at dosages and for periods of time necessary, to achieve the desired therapeutic result, such as reduced viral load, increased life span or increased life expectancy. A therapeutically effective amount of a compound may vary according to factors such as the disease state, age, sex, and weight of the subject, and the ability of the compound to elicit a desired response in the subject. Dosage regimens may be adjusted to provide the optimum therapeutic response. A therapeutically effective amount is also one in which any toxic or detrimental effects of the compound are outweighed by the therapeutically beneficial effects. A “prophylactically effective amount” refers to an amount effective, at dosages and for periods of time necessary, to achieve the desired prophylactic result, such as less severe infection or delayed or no onset, increased life span, increased life expectancy or prevention of the progression of infection. Typically, a prophylactic dose is used in subjects prior to or at an earlier stage of disease, so that a prophylactically effective amount may be less than a therapeutically effective amount.
It is to be noted that dosage values may vary with the severity of the condition to be alleviated. For any particular subject, specific dosage regimens may be adjusted over time according to the individual need and the professional judgment of the person administering or supervising the administration of the compositions. Dosage ranges set forth herein are exemplary only and do not limit the dosage ranges that may be selected by medical practitioners. The amount of active compound(s) in the composition may vary according to factors such as the disease state, age, sex, and weight of the subject. Dosage regimens may be adjusted to provide the optimum therapeutic response. For example, a single bolus may be administered, several divided doses may be administered over time or the dose may be proportionally reduced or increased as indicated by the exigencies of the therapeutic situation. It may be advantageous to formulate parenteral compositions in dosage unit form for ease of administration and uniformity of dosage.
In some embodiments, compounds and all different forms thereof as described herein may be used, for example, and without limitation, in combination with other treatment methods.
In general, compounds described herein should be used without causing substantial toxicity. Toxicity of the compounds of the invention can be determined using standard techniques, for example, by testing in cell cultures or experimental animals and determining the therapeutic index, i.e., the ratio between the LD50 (the dose lethal to 50% of the population) and the LD100 (the dose lethal to 100% of the population). In some circumstances, however, such as in severe disease conditions, it may be necessary to administer substantial excesses of the compositions. Some compounds described herein may be toxic at some concentrations. Titration studies may be used to determine toxic and non-toxic concentrations. Toxicity may be evaluated by examining a particular compound's or composition's specificity across cell lines Animal studies may be used to provide an indication if the compound has any effects on other tissues.
Compounds as described herein may be administered to a subject. As used herein, a “subject” may be a human, non-human primate, rat, mouse, cow, horse, pig, sheep, goat, dog, cat, etc. The subject may be suspected of having or at risk for having an infection, such as viral infection, or suspected of having or at risk for having viral infection. In particular, the infection may mediated by a neuraminidase. Diagnostic methods for viral infection, such as influenza and the clinical delineation of viral infection, such as influenza are known to those of ordinary skill in the art.
TABLE 1 Compounds made and tested for neuraminidase modulatory activity.







TABLE 1







2,3-Fluorinated Glycosides










Compound
Structure






4 (N3-100ACD)






 



5 (N3-105ET)






 



12 (N3-106GU)






 



13 (N3-106ET)






 



7 (N3-102ET)






 



9 (N3-111AMD)






 



8 (N3-109N3)






 



11 (N3-107AC)






 



23DFSA 2,3- Difluorosialic acid






 



C4 Bn













TABLE 2A Compounds having neuraminidase modulatory activity.







TABLE 2A







2,3-Fluorinated Glycosides with Neuraminidase Modulatory Activity








Compound
Structure





4 (N3-100ACD)






 


5 (N3-105ET)






 


12 (N3-106GU)






 


7 (N3-102ET)






 


9 (N3-111 AMD)






 


13 (N3-106ET) 






 


C4 Bn






 


8













TABLE 2B Compounds made and expected to have neuraminidase modulatory activity.







TABLE 2B





2,3-Fluorinated Glycosides Compounds













 








 








 








 








 








 








 








 








 















Compounds described herein may also be used in assays and for research purposes. Compounds for use in the present methods may be synthesized using the methods described herein.
Various alternative embodiments and examples are described herein. These embodiments and examples are illustrative and should not be construed as limiting the scope of the invention.
Previous published work by Hagiwara et al (1994) reported 3-fluoro-sialic acids as being only modest sialidase inhibitors. Specifically, they report two compounds, one with an OH group at carbon 2 (position Z in Formula I). However, the OH group is not a sufficient leaving group to allow trapping of a covalent intermediate. Accordingly, the Hagiwara et al. OH compound (at C2 equivalent to Z in Formula I) showed minimal inhibition. Furthermore, the other compound tested by Hagiwara et al., which has a fluorine (a sufficient leaving group) at C2 (equivalent to Z in Formula I), did not have the correct stereochemistry. Accordingly, an appreciation of these requirements was missing in Hagiwara et al.
General Methodologies
Synthesis
General methodologies for chemical preparation of compounds of Formula I are described in the following non-limiting exemplary schemes.




Selectfluor (3.5 eq.) was added to a solution of SAN3 (1 eq.) in MeNO2/water (3/1 ̃4/1) and the solution stirred for 3 days or more to complete the reaction at room temperature (Synthesis of SAN3—Chandler, M. et al. Journal of the Chemical Society-Perkin Transactions 1, 1995; 1173-1180). The reaction mixture was quenched with saturated NaHCO3 and extracted with EtOAc. Presence of compound (1) can easily be confirmed via TLC. The axial-F (1) has a lower Rf value than the starting material, and the equitoral-F compound and any other stereoisomers (anomeric hydroxy isomers) would have higher Rf values than the starting material. The fluoride at C3 was detected at −204 ppm on 19F NMR experiment, and the stereochemistry was assigned on the basis of 1H- and 19F-NMR coupling constants. The coupling constants JH3/F3 (47.9 Hz) and JF3/H4 (31.0 Hz) are indicative of an axial configuration of the fluorine atoms at C3, respectively.







TABLE 3







Examination for the fluorination at C3 with Selectfluor.













Time

Selectivity



condition
(Day)
Yields
(axial/equitorial)















1
MeNO2/water (3/1), rt
3
75%
5/1


2
MeNO2/water (3/1), 60° C.
2
62%
3/1


3
DMF/water (8/1), 80° C.
>30
40%
2/1


4
MeCN/water (8/1), 60° C.
>30
40%
2/1









The reaction can be monitored for completion by UV on TLC, because only the starting material is detected under short UV. The reaction is considered complete upon disappearance of the UV active compound.




DAST (1.1 eq.) was added drop wise to a suspended solution of compound 1 (1 eq.) in DCM −40° C. and the solution stirred vigorously for 30 min. After the mixture turned clear, the reaction mixture was quenched with saturated NaHCO3 and extracted with DCM and EtOAc to give compound 2. If the reaction mixture doesn't turn clear after 20 min, the reaction mix is placed in a −10° C. bath for the last 10 min.
Compound 2 was hydrogenated with Pd/C in MeOH overnight at room temperature, the catalyst was then filtered off and 6M NaOMe was added to the reaction mixture. The mixture was then acidified with IR120 (H+, strong) to remove the Na+. The resin was filtered off and the filtrate was evaporated and chromatographed (EtOAc/MeOH/water=15/2/1). The hydrogenation was monitored by staining with ninhydrine solution. Both compounds 3 and 4 were isolated.




To a solution of compound 3 in MeCN/water (5/1) was added acetaldehyde (2 eq) at room temperature. After 30 min, 4 eq. NaCNBH3 was added and the reaction mixture stirred for 10 min at the same temp. The reaction mixture was quenched with 5% citric acid and chromatographed with EtOAc/acetone (9/1).
The monoacetylated amine (6) was deprotected with 6M NaOMe in wet MeOH to give compound 7.




Compound 4 was re-esterified with catalysis HCl (2M HCl in Et2O) in dry EtOH at room temperature. The reaction mixture was left over night and then evaporated. 2 eq. HCl was usually added. The ethyl ester can be separated by silica gel chromatography.




The difluorinated compound 2 was deprotected with 6M NaOMe in wet MeOH at room temperature, and compound 8 was easily purified by silica gel chromatography as 90% yields after acidification with IR120 (strong H+).




The azide 2 was hydrogenated with Pd/C to give compound 3, then NH3(g) was bubbled to remove acetate and to give amide 4 with good yield.




For the preparation of compound 11, the amine 3 was acetylated with Ac2O in pyridine, and O-acetylates and methyl ester were selectively removed with NaOMe. N-acetylated compound 11 was purified and obtained in 70% yield (three steps overall).




The guanylated compound 12 was prepared with 3,5-dimethylpyrazole-1-carboxamidine and Et3N in MeOH at 60° C. for 3 weeks. The reaction mixture was quenched after three weeks, and 50% of the starting material 4 was successfully recovered. The 4-guanylated 12 was isolated as 80% yield, and the ethylester 13 was given as 80% yield after re-esterification with catalytic HCl in EtOH at room temp for 5 hours.
Compounds of Formula I may be prepared by the chemical methodologies described in the following non-limiting exemplary scheme.




Synthesis of 4-amino derivatives of 2,3-difluorosialic acid. Reagents and conditions are as follows: Ref 1 is Chandler el al. (1995) J Chem Soc. Perk. Trans 1, 1173-1180; (a) 4eq. Selectfluor, MeNO2/water (3/1), rt.; (h) DAST, DCM, −40° C. (c) Na0Me, wet Me0H, rt.; (d) Pd/C, H2, Me0H, rt.; (e) EtOH, cat. FICI, rt.; (f) Acetaldehyde for 4, Benzaldehyde for 5, NaCNBH3, acetone, it and (g) 3,5-dimethylpyrazole-1-carboxamidine nitrate, MeOH, 60° C.

Compounds of Formula I having an amine at C4, may alternatively be prepared by the chemical methodologies described in the following non-limiting exemplary scheme.




Alternatively, compounds of Formula I having a guanidine at C4, may be prepared by the chemical methodologies described in the following non-limiting exemplary scheme.




Alternatively, compounds of Formula I may be prepared by the chemical methodologies described in the following non-limiting exemplary scheme.




Alternatively, compounds of Formula I having modifications at C1, may be prepared by the chemical methodologies described in the following non-limiting exemplary scheme.




It will be appreciated by a person of skill in the art, that variations in the alkyl chain length may be achieved by substituting 1-octanol (C8—having 8 carbons) for an alternative alcohol. For example, 1-octanol in the above scheme may be substituted for an alternative primary alcohol, which may, for example, be selected from one or more of the following: Propan-1-ol (C3); Butanol (C4); 1-Pentanol (C5); 1-Hexanol (C6); 1-Heptanol (C7); 1-Nonanol (C9); 1-Decanol (C10); Undecanol (C11); Dodecanol (C12); 1-Tetradecanol (C14); Cetyl alcohol (C16); Stearyl alcohol (C18); and Arachidyl alcohol (C20). Similarly, it will be appreciated that an alternative substrate for this reaction may be chosen. For example, instead of DFSA-4NH2 (compound 4), compound 12 (DFSA-4Gu), or compound 7, etc. may be substituted.
Alternatively, compounds of Formula I having modifications at Cl, may be prepared by the chemical methodologies described in the following non-limiting exemplary scheme. For example, a hydrochloric acid salt of ethyl 5-acetamido-4-guanyl-2,3,4,5,-tetradeoxy-3-fluoro-D-erythro-β-L-manno-non-2-ulopyranosylonate fluoride is shown below, which also adds an ethyl group at C1 (R1). It will be appreciated by a person of skill in the art, that variations in the salt produced may be achieved by substituting an alternative acid and that the length of the alkyl group at C1 may be adjusted by substituting an alternative alcohol as set out above.




Alternatively, compounds of Formula I having modifications at ring C6, may be prepared by the chemical methodologies described in the following non-limiting exemplary schemes.




It will be appreciated by a person of skill in the art, that variations in the alkyl chain length may be achieved by substituting either Trimethyl orthooctyrate (C8—having 8 carbons) or Trimethyl orthobutyrate (C4—having 4 carbons) for an alternative ortho ester derivative. For example, Trimethyl orthooctyrate or Trimethyl orthobutyrate in the above schemes may be substituted for an alternative ortho ester derivative, which may, for example, be selected from one or more of the following: Trimethyl orthoacetate (C2); Trimethyl orthopropionate (C3); Trimethyl orthopentionate (C5); Trimethyl orthohexanate (C6); Trimethyl orthoheptanate (C7); Trimethyl orthononate (C9); Trimethyl orthodecanate (C10). Similarly, it will be appreciated that an alternative substrate for this reaction may be chosen.
Characterization
(2R,3R)-4-Azido-4-deoxy-3-fluoro-7,8,9-tri-O-acetyl-sialic acid methyl ester (1)
ESI-MS m/z 515.1 (+Na); 19F-NMR (CFCl3, 282 MHz) δ −204.7 (dd, J 47.9 and 31.0 Hz); 1H NMR (300 MHz) δ 5.32 (1H, m); 5.14 (1H, m); 5.00 (1H, dd, J 31.4 and 1.5 Hz), 4.86 (1H, dd, J 4.5 and 1.9 Hz), 4.25 (1H, m), 4.15 (1H, m), 4.04 (1H, dd, J 12.5 and 8.5 Hz), 3.83 (1H, m), 3.77 (3H, OMe), 2.02 (12H, 4s, 4 Ac)
(2R,3R)-4-Azido-2,4-dideoxy-2,3-difluoro-7,8,9-tri-O-acetyl-sialic acid methyl ester (2)
ESI-MS m/z=517.0 (+Na); 19F-NMR (CFCl3, 282 MHz) δ −123.2 (1F, t, 8.46 Hz), −217.2 (1F, m); 1H-NMR (300 MHz) δ 7.23 (1H, d, NH), 5.97 (1H, m), 5.37 (1H, m), 5.24 (1H, m), 5.10 (1H, dd, J 49.4 and 7.3 Hz), 4.65 (1H, dt, 28.1 and 9.4 Hz), 4.51 (1H, m), 4.27 (2H, m), 3.88 (3H, d, J 7.0 Hz, OMe), 3.39 (1H, m), 2.10 (12H, m, 4Ac). 13C NMR (100 MHz) δ 21.6, 21.7, 21.8, 24.5, 49.1, 54.7, 57.8 (dd), 62.7, 68.5, 69.6, 71.6 (d), 78.2, 87.5 (dd), 105.5 (dd), 165.0 (dd), 170.6, 171.4, 172.2, 172.5
(2R,3R)-4-Azido-2,4-dideoxy-2,3-difluorosialic acid (8)
ESI-MS m/z=353.2 (−H); F-NMR (CFCl3, 282 MHz) δ −122.3 (1F, d, J 11.3 Hz), −216.4 (1F, ddd, J 50.1, 29.2 and 11.3 Hz); 1H-NMR (300 MHz) δ 5.30 (1H, dm, J 50.2 Hz), 4.34 (1H, t, J 10.8 Hz), 4.07 (1H, dd, J 29.1 and 11.2 Hz), 3.90 (1H, d, J 10.5 Hz), 3.74 (2H, m), 3.50 (3H, m), 1.95 (3H, s, Ac). 13C NMR (100 MHz) δ 23.0, 45.9 (d), 61.5 (dd), 63.9, 68.7, 71.1, 74.1 (d), 88.0 (dd), 106.5 (dd), 169.2 (dd), 175.8
(2R,3R)-4-Amino-2,4-dideoxy-2,3-difluorosialylamide (9)
ESI-MS m/z=350.1 (+Na); 19F-NMR (CFCl3, 282 MHz) δ−121.1 (1F, d, J 8.46 Hz), −219.4 (1F, m); 1H-NMR (300 MHz) δ 5.10 (1H, dm, J 49.3 Hz), 4.20 (1H, t, J 10.8 Hz), 3.96 (1H, d J 10.6 Hz), 3.73 (2H, m), 3.50 (3H, m), 1.95 (3H, d, J 1.2 Hz, Ac). 13C NMR (100 MHz) δ 22.2, 46.1, 51.5 (dd), 63.2, 67.5, 70.1, 74.1, 87.0 (dd) 105.0 (dd), 168.2 (dd), 175.2
(2R,3R)-4-(N-Acetyl) amino-2,4-dideoxy-2,3-difluorosialic acid (11)
ESI-MS m/z=415.1 (+2 Na); 19F-NMR (CFCl3, 282 MHz) δ −121.8 (1F, d, J 11.9 Hz), −214.4 (1F, m); 1H-NMR (300 MHz) δ 5.05 (1H, d, J 50.5 Hz), 4.50 (1H, m), 4.18 (1H, m), 4.05 (1H, d J 10.4 Hz), 3.82 (1H, d, J 10.3 Hz), 3.75 (2H m), 3.48 (2H, m), 1.89 (3H, s, Ac), 1.84 (3H, s, Ac). 13C NMR (100 MHz) δ 21.6, 21.8 (d), 45.2, 50.8 (m), 63.1, 68.0, 70.5, 70.6, 73.3 (d), 73.5, 169.5 (dd), 174.2, 174.8
(2R,3R)-4-(N-Ethyl) amino-2,4-dideoxy-2,3-difluorosialic acid (7)
ESI-MS m/z=355.3 (−H); 19F-NMR (CFCl3, 282 MHz) δ −121.8 (1F, d, J 11.5 Hz), −216.4 (1F, m); 1H-NMR (300 MHz) δ 5.30 (1H, dm, J 50.2 Hz), 4.29 (1H, t, J 10.7 Hz), 3.82 (1H, d J 10.6 Hz), 3.73 (2H, m), 3.52 (3H, m), 3.00 (2H, m), 1.95 (3H, s, Ac), 1.10 (3H, t, J 7.3 Hz). 13C NMR (100 MHz) δ 12.4, 23.2, 41.3, 45.2, 58.0 (dd), 63.9, 64.2 (d), 68.7, 71.2, 74.2 (d), 85.5 (dd), 107.2 (dd), 170.0 (dd), 176.1
(2R,3R)-4-Amino-2,4-dideoxy-2,3-difluorosialic acid (4)  
ESI-MS m/z=351.2 (+Na); 19F-NMR (CFCl3, 282 MHz) δ −122.0 (1F, d, J 11.3 Hz), −217.4 (1F, m); 1H-NMR (400 MHz) δ 5.10 (1H, ddt, J 50.4, 10.0 and 5.2 Hz), 4.16 (1H, m), 3.73 (3H m), 3.45 (3H, m), 1.91 (3H, dd, J 5.1 and 1.4 Hz, Ac). 13C NMR (100 MHz) δ 23.1, 46.8, 53.1 (dd), 63.9, 64.1, 68.5, 71.4, 74.4, 89.8 (dd), 108.5 (dd), 170.4 (dd), 176.0
Ethyl (2R,3R)-4-Amino-2,4-dideoxy-2,3-difluorosialylate (5)
ESI-MS m/z=379.1 (+Na); 19F-NMR (CFCl3, 282 MHz) δ −122.3 (1F, d, J 5.6 Hz), −219.0 (1F, m); 1H-NMR (400 MHz) δ 5.10 (1H, ddt, J 49.2, 10.0 and 2.8 Hz), 4.45 (2H, m), 4.14 (1H, m), 4.05 (1H, d J 10.4 Hz), 3.88 (3H m), 3.66 (3H, m), 2.10 (3H, d, J 1.6 Hz, NAc), 1.30 (3H, dt, J 70.4 and 7.2 Hz). 13C NMR (100 MHz) δ 13.4, 17.1, 22.4, 44.6 (dd), 52.4 (dd), 57.7, 63.2, 65.0, 67.7 (d), 70.1 (d), 73.3 (d), 86.7 (dm), 105.1 (tm), 167.0 (dm), 175.4 (d)
(2R,3R)-4-Guanyl-2,4-dideoxy-2,3-difluorosialic acid (12)
ESI-MS m/z=370.3 (−H); 19F-NMR (CFCl3, 282 MHz) δ −121.3 (1F, d, J 14.4 Hz), −214.7 (1F, m); 1H-NMR (300 MHz) δ 5.00 (1H, dm, J 50.2 Hz), 4.19 (1H, t, J 8.9 Hz), 3.81 (2H, m), 3.52 (3H, m), 3.10 (1H, q, J 7.3 Hz), 1.90 (3H, s, Ac), 1.12 (4H, m). 13C NMR (100 MHz) δ 22.3, 43.5, 46.5, 55.1, 63.9, 68.9, 71.5, 74.2, 89.5 (dd), 107.0 (dd), 161.5, 170.1 (dd), 175.8,
Ethyl (2R,3R)-4-Guanyl-2,4-dideoxy-2,3-difluorosialylate (13)
ESI-MS m/z=421.4 (+Na); 19F-NMR (CFCl3, 282 MHz) δ −122.5 (1F, d, J 12.0 Hz), −216.0 (1F, m); 1H-NMR (300 MHz) δ 5.16 (1H, dm, J 49.5 Hz), 4.32 (2H, m), 4.18 (2H, m), 4.03 (1H, d, J 9 Hz), 3.70 (2H, m), 3.50 (3H, m), 3.09 (2H, q, J 7.1 Hz), 1.90 (3H, s, Ac), 1.23 (3H, t, J 6.2 Hz).




Hydrochloric acid salt of octyl 5-acetamido-4-amino-2,3,4,5,-tetradeoxy-3-fluoro-D-erythro-β-L-manno-non-2-ulopyranosylonate fluoride
ESI-MS m/z=463.3 (M+Na); 1H-NMR (400 MHz, CH3OD) δ 5.37 (1H, app dt, J 49.92, 5.04), 4.50-4.44 (1H, m), 4.38-4.27 (2H, m), 4.21-4.11 (2H, m), 3.80-3.77 (1H, m), 3.75 (1H, dd, J 6.91, 2.30), 3.69-3.66 (1H, m), 3.60-3.55 (1H, m), 2.04 (3H, s), 1.80-1.73 (2H, m), 1.42-1.28 (10, m), 0.91 (3H, t, J 7.00).




Hydrochloric acid salt of ethyl 5-acetamido-7,8,9-tri-O-acetyl-4-amino-2,3,4,5-tetradeoxy-3-fluoro-D-erythro-β-L-manno-non-2-ulopyranosylonate fluoride
ESI-MS m/z=483.3 (M+H); 1H-NMR (400 MHz, CDCl3) δ 6.99 (1H, s), 5.70-5.23 (3H, m), 5.02 (1H, s), 4.61-4.08 (6H, m), 2.28-1.23 (15H, m).




Ethyl 5-acetamido-4-amino-2,3,4,5,-tetradeoxy-3-fluoro-D-erythro-β-L-manno-non-2-ulopyranosylonate fluoride
ESI-MS m/z=379.1 (M+Na); 1H-NMR (400 MHz, D2O) δ 5.18 (1H, app d, J 49.34), 4.38 (2H, q, J 7.16), 4.20-4.14 (1H, m), 4.01 (1H, d, J 10.51), 3.87-3.76 (2H, m), 3.61 (1H, dd, J 11.95, 5.86), 3.55 (1H, d, J 9.29), 3.39 (1H, dd, J 30.00, 10.96), 2.03 (3H, s), 1.32 (3H, t, J 7.16).




Hydrochloric acid salt of 5-acetamido-4-amino-9-butyroyl-2,3,4,5,-tetradeoxy-3-fluoro-D-erythro-β-L-manno-non-2-ulopyranosylonate fluoride
ESI-MS m/z=421.3 (M+Na); 1H-NMR (400 MHz, MeOD) δ 5.34 (1H, app d, J 51.62), 4.47 (1H, app t, J 10.66), 4.36 (1H, d, J 10.20), 4.16 (1H, dd, J 11.42, 6.24), 4.09-3.97 (3H, m), 3.55 (1H, d, J 9.14), 2.34 (2H, t, J 7.31), 2.03 (3H, s), 1.70-1.61 (2H, m), 0.96 (3H, t, J 7.31).




Hydrochloric acid salt of 5-acetamido-4-amino-9-octanoyl-2,3,4,5,-tetradeoxy-3-fluoro-D-erythro-β-L-manno-non-2-ulopyranosylonate fluoride
ESI-MS m/z=453.3 (M−H); 1H-NMR (400 MHz, MeOD) δ 5.35 (1H, app d, J 45.84), 4.58-4.28 (2H, m), 4.23-3.88 (4H, m), 3.63-3.50 (1H, m), 2.48-2.26 (2H, m), 2.15-1.94 (3H, m), 1.72-1.54 (2H, m), 1.51-1.14 (8H, m), 1.03-0.88 (3H, m).




Hydrochloric acid salt of ethyl 5-acetamido-4-guanyl-2,3,4,5,-tetradeoxy-3-fluoro-D-erythro-β-L-manno-non-2-ulopyranosylonate fluoride
ESI-MS m/z=421.4 (M+Na); 1H-NMR (300 MHz, D2O) □ 5.16 (1H, app d, J 49.5 Hz), 4.32 (2H, m), 4.18 (2H, m), 4.03 (1H, d, J 9 Hz), 3.70 (2H, m), 3.50 (3H, m), 3.09 (2H, q, J 7.1 Hz), 1.90 (3H, s), 1.23 (3H, t, J 6.2 Hz).
Enzyme Kinetics
All experiments were carried out in 20 mM Tris/50 mM CaCl2 buffer, pH 7.6 containing 0.1% BSA. Cuvettes had a path length of 1 cm and were used in either Cary 4000 or Cary 300 UV/visible spectrophotometer connected to a circulating water bath. The data were analyzed using the program GraFit 4.0 (Erithacus software). Time-dependent inactivations were performed by pre-incubating the enzyme at 30° C. in the presence of several concentrations of inactivator. Residual enzyme activity was determined at appropriate time intervals by the addition of an aliquot of the inactivation mixture to an assay solution containing 0.5 mM 4-trifluoromethylumbelliferyl sialic acid (CF3MUSA). Kinetic parameters were determined by measuring the initial linear increase in absorbance at 385 nm. The initial rates at each time point were plotted as a function of time to obtain time-dependent exponential decay curves from which ki obs could be obtained for each inactivator concentration using the equation:

(rate)t=(rate)t=0e(ki obs t)+offset.

The offset was used because the rates did not decay to zero. The inactivation rate constant (ki) and the reversible dissociation constant for the inactivator (Kd) were determined by plotting ki obs versus inactivator concentration to the equation:

ki obs=ki[I]/(Kd+[I]).

In the case [I]<<Kd, a second-order rate constant (ki/Kd) was determined by fitting the data to the equation:

ki obs=ki[I]/Kd.

Time-dependent reactivations were performed by applying the inactivated enzyme solution (50 μl) to AmiconTM 10 K filter (MilliporeTM) to remove excess inactivator. The filter was washed 5-times with 150 μL buffer at 4° C. Enzyme activity was assayed at time intervals by the addition of an aliquot of eluted enzyme to an assay solution containing 0.5 mM CF3MUSA. First-order rate constants for reactivation at each acceptor concentration (kr obs) were determined by direct fit of the activity versus time data to a first-order equation. Reactivation experiments were attempted but no significant enzyme activity could be detected over time, suggesting that the hydrolysis of the sialyl-enzyme intermediate is very slow. The Ki was determined by fitting the data to the equation:

Ki=Kd(khyd/ki).

Cell-Based Assay of Influenza Anti-Viral Activity

Compounds were tested for antiviral activity using a cell based assay, which consists of making serial 2-fold dilutions of the antiviral compounds (from 1:2 to 1:4096 in MegaVir medium in enough volume for the number of viruses tested—60 uL per virus), to which is added 100 infectious units of the specific influenza virus and the preparations are transferred to monolayers of MDCK cells in a microtitre plate. The assay was carried out on a 96-well microtitre plate. The plate is monitored for the development of influenza cytopathic effects from days 3 to 5 post infection. Antiviral activity is determined by the inhibition of development of cytopathic effects. The highest dilution of the compound at which the monolayers are intact is taken as the end-point. Zanamivir was used as a positive control.
Dilution Preparations:


    
    
        1. In row A on a clean 96-well microtitre plate, prepare 2-fold serial dilutions of antiviral compounds from 1:2 to 1:4096 in MegaVir medium in enough volume for the number of viruses tested (60 uL per virus).
        2. Transfer 55 uL of the 2-fold dilution series to a clean row in the 96-well microtitre plate.
        3. To the 55 uL dilution series, add 55 uL of diluted influenza virus (at 100 TCID50 per 25 ul). Also add virus to positive control wells.
        4. To the now 110 uL mixture, add 55 uL of 4X TPCK-treated trypsin. Add trypsin also to positive and negative control wells. Mix well.
        5. Prepare also 2-fold serial dilutions from 1:2 to 1:256 for the inoculating virus in MegaVir medium for back titration.

Plate Inoculation:

        6. In a 96-well microtitre plate containing confluent monolayers of MDCK cells in  ̃200 uL MegaVir medium, transfer 75 uL of the mixture to 2 respective rows as duplicates.
        7. Transfer 50 uL of the positive control, and 25 uL of negative controls to respective wells.
        8. Transfer also 25 uL of the virus back titration in duplicates.
        9. Therefore in each well:
        
            a. Samples: 25 uL compounds+25 uL virus+25 uL trypsin
            b. Positive control: 25 uL virus+25 uL trypsin (no compounds)
            c. Negative control: 25 uL trypsin (no compounds or virus)
            d. Back titration: 25 uL virus
        
        
        10. The plates are incubated at 37 C in a CO2 incubator for 3 days, then observed for the appearance of cytopathic effects on day 3 and day 5.

In Vivo Pharmacokinetic (PK) Profile Study

Dose Administration

    
    


Intravenous (IV) injections—Mice were injected with the required volume to administer the prescribed dose (mg/kg) to the animals based on individual mouse weight using a 28G needle. The injection volume was 200 μL/20 g mouse. The mice were briefly restrained during IV injections for approx. 1 minute. Dilation of the vein was achieved by holding the animals under a heating lamp for a period of between 1-2 minutes.
Intra-nasal (IN) administration—Mice were anesthetized with isoflurane 2% and 2 L/02/min until the absence of a toe pinch reflex. Animals were restrained in an upright position and using a micropipette, animals were instilled with 104 of the compounds into each nostril per 15 g of body weight. The mice were briefly restrained during the instillation procedure for approx. 20 seconds and the rate of release was adjusted to allow the mice to inhale the compounds without forming bubbles. The mouse was placed back in the anesthetic chamber in an inverted position for an additional 2 minutes or until breathing returned to normal.
Pharmacokinetic Sampling
Mice were individually weighed and evenly distributed into groups according to weight. Mice (n=20/group and n=4/time point) were injected with the test articles as described in the dose administration section.
Blood collection: Blood was collected at the time points indicated in the study grouping table. For blood collection, mice were terminated by CO2 inhalation and blood was collected by cardiac puncture. Upon last breath, mice were removed from inhalation chamber and approx 500-700 μL of blood was collected by cardiac puncture with a 25G needle and placed into the appropriate EDTA microtainer tube. Each tube was inverted several times to ensure even mixing of blood and EDTA to prevent coagulation. Blood samples were stored on ice until all samples were collected for a particular time point and will then further processed to generate plasma.
Plasma preparation: Plasma was prepared by centrifuging samples at 2500 rpm for 15 minutes at 4° C. (rpm based on Beckman GH 3.8 A rotor, RCFavg 250×g), then pipetted off and placed into labelled vials on ice and then frozen at −80° C. Samples were shipped on dry ice.
Tissue Collections: After blood collection the trachea and lung tissue were harvested. Briefly, the ventral side of the neck was cut to expose the thyroid and sternothyroid muscles. The muscles were gently teased apart to expose the larynx and trachea, A hemostat was used to close off the trachea (near the larynx) which then was cut just posterior to the larynx and the entire trachea, bronchial tree was removed with the lungs attached. The trachea was separated from the lungs. Tissue was not rinsed in saline. Each tissue sample was transferred to an individual labeled vial on ice and then frozen at −80° C. Samples were shipped on dry ice.
Observation of Animals
Mice were continually monitored for acute signs of toxicity for the first two hours following test compound administration. For groups of mice in the last time point (7 days), mice were monitored 2× daily prior to sacrifice and tissue collection. Body weights of individual mice were measured every Monday, Wednesday and Friday over the course of the study.
Data Collection
Actual time of blood collection (time of day), body weights and behavioural parameters as described in Experimental Design: Pharmacokinetic Sampling. The following records were collected:

    
    
        Manual Randomization by body weight
        Individual body weights
        Observations
        Comments
        Actual time of blood & tissue collection
        Identity of tissue samples
        Reasons/findings related to any premature termination of animals

Observations of Animals

Evaluation of Drug-Induced Stress—All animals were observed post administration, and at least once a day, more if deemed necessary, during the pre-treatment and treatment periods for mortality and morbidity. In particular, signs of ill health were based on body weight loss, change in appetite, and behavioral changes such as altered gait, lethargy and gross manifestations of stress. When signs of severe toxicity were seen, the animals were terminated (CO2 asphyxiation) and a necropsy was performed to assess other signs of toxicity. The following organs were examined: liver, gall bladder, spleen, lung, kidney, heart, intestine, lymph nodes and bladder. Any other unusual findings were also noted.

    
    


Moribund animals were terminated for humane reasons and the decision to terminate was at the discretion of the animal care technician and the study director. These findings were recorded as raw data and the time of death will be logged on the following day.
In Vivo Mouse Model of Influenza
Animals
A mouse model of influenza was used for this study. 6-7 week old female mice (Balb/C—Mus musculus), Mice were given adapted HK1 influenza virus at 1250 pfu per mouse. Challenge doses were 3×LD50 determined in vivo titration studies. Animals were housed in level 2 containment.










Sequence of activities in the study








Time











Week
Day
Hour
Activity













1
1
−2
First treatment




0
Inoculate with virus




4
Second treatment





Observe animals twice daily



2
16
Third treatment




24
Fourth treatment





Observe animals twice daily



3
40
Fifth treatment




48
Sixth treatment





Observe animals twice daily



4
64
Seventh treatment




72
Eighth treatment





Observe animals twice daily



5
88
Ninth treatment




96
Tenth treatment





Observe animals twice daily



6
112
Eleventh treatment




120
Twelfth treatment





Observe animals twice daily


2
 7-14

Observe animals daily


3
14-21

Observe animals daily









Intranasal Dose Administration

Mice were anesthetized with isoflurane 2% and 2 L/02/min until the absence of a toe pinch reflex. Animals were restrained in an upright position and using a micropipette, animals were instilled with 10 μL of the compounds into each nostril. The mice were briefly restrained during the instillation procedure for approx. 20 seconds and the rate of release should be adjusted to allow the mice to inhale the compounds without forming bubbles. The mice were placed back in the anesthetic chamber in an inverted position for an additional 2 minutes or until their breathing returned to normal.
Intranasal Virus Inoculation
Mice were anesthetized with isoflurane 2% and 2 L/02/min until the absence of a toe pinch reflex. Animals were restrained in an upright position and using a micropipette, animals were instilled with 10 μL of a virus preparation containing 3,000 pfu of influenza A virus, A/HK/1/68 (H3N2) in to each nostril (total inoculum of 1,250 pfu per animal). The virus was prepared as a suspension in serum-free DMEM. The mice were briefly restrained during the instillation procedure for approx. 20 seconds and the rate of release should be adjusted to allow the mice to inhale the compounds without forming bubbles. The mice were placed back in the anesthetic chamber in an inverted position for an additional 2 minutes or until breathing returned to normal.
Data Collection
The following records were collected:


    
    
        Manual randomization by body weight
        Individual body weights
        Observations
        Comments
        Reasons/findings related to any premature termination of animals

Evaluation of Drug or Disease Induced Stress

    
    


All animals were observed post administration, twice a day during treatment periods and once every day thereafter for mortality and morbidity. Signs of ill health included body weight loss, change in appetite, difficulty in breathing and behavioral changes such as altered gait/posture, lethargy and gross manifestations of stress. At the sign of severe illness (determined by >15% weight loss), the animals were called endpoint and terminated (CO2 asphyxiation).
Moribund animals were terminated for humane reasons and the decision to terminate was at the discretion of the animal care technician and the study director. These findings were recorded as raw data and the time of death were logged on the following day.
EXAMPLES
Further embodiments are described with reference to the following, non-limiting, examples.
Example 1
Viral Sialidase Enzyme Assays
Incubation of influenza sialidase with different concentrations of the various compounds resulted in time-dependent decreases in enzyme activity, as expected for mechanism-based inhibitors, and as shown for compound 4 in FIGS. 3A and B. FIGS. 3A and 3B show time-dependent inactivation of influenza sialidase (subtype N9) by compound 4. The enzyme was incubated with the indicated concentrations of compound 4, and aliquots assayed with 0.5 mM CF3MUSA. Inactivation by compound 4 at indicated concentration (3A), and re-plot of pseudo-first order inactivation kinetic constants (ki obs) versus concentration of compound 4 (3B).
All 3-fluorosialyl fluorides showed excellent inactivation profiles against influenza sialidase at 30° C. except compound 8, and the half time for the full inactivation of the sialidase was estimated as ca. 10 min for all amine derivatives (compounds 4, 7, 9, 11, and 12) (summary in Table 4). Interestingly, 4-aminated compound 4 and 4-guanylated compound 12 showed good inactivation values (ki/Kd=25 min−1mM−1 for compound 4 and ki/Kd=24 min−1mM−1 for compound 12). Furthermore, compounds 4 and 12 showed slow reactivation in buffer solution at the same temperature with the half time for the full reactivation of the inactivated sialidase being determined as 4.8 h for compound 4 and 6.7 h for compound 12. Accordingly, the 3-fluorosialylenzyme intermediate very stably blocked the active site in influenza sialidase and their effective Kis were in the nanomolar range (93 nM for compound 4 and 70 nM for compound 12).
Inactivation parameters for 2,3-Difluorosialic acid, (23DFSA) and the 4-azide compound 8 could not be measured at 30° C. due to fast hydrolysis of sialylenzyme intermediate. Thus, the kinetic values for these two inhibitors were measured at 4° C., and the estimated inactivation numbers were given. The ki/Kd value of compound 23DFSA was determined as 196 min−1mM−1, and 4-azide compound 8 and 4-amine compound 4 showed 26-fold lower kinetic values at 4° C. (ki/Kd=7.5 min−1mM−1 for compound 8 and 7.3 min−1 mM−1 for compound 4). Although the 4-amine derivatives showed lower inactivation kinetic values, these compounds were revealed as much better inhibitors than the 4-hydroxylated derivative (23DFSA) due to their very slow reactivation.







TABLE 4







Kinetic parameters for the reaction of influenza


sialidase with 3-fluorosialyl fluorides.a













ki/Kd
Kd
t1/2 (inactivation)
khyd
Ki


Compound
(min−1mM−1)
(mM)
(min)
(min−1)
(nM)





23DFSA
196b     
NDb
NDb
 NDb
 NDb


C4 Bn
0.61
0.306
3.7
0.0025
4080


8
7.5b
0.240b
0.4b
 NDb
 NDb


9
0.34
1.016
1.9
ND
ND


11
0.53
0.397
3.2
ND
ND


7
4.1 
0.014
11.7
ND
ND


4
25 (7.3)b
0.004
6.4
0.0024
93.2


12
24   
0.009
3.1
0.0017
70.4





aAll the experiments were carried out in 20 mM TRIS/50 mM CaCl2 buffer, pH 7.6 containing 0.1% BSA at 30° C.


bThe kinetic values were collected at 4° C.


ND = not determined.






Example 2
Human Sialidase Enzyme Assays
Incubation of different concentrations of each compound with human sialidase resulted in no inactivation of the human sialidase even at very high concentrations (10 mM) of compound demonstrating the specificity of the compounds for influenza sialidases.
Example 3
Cytoprotection Assays with Influenza A Strains
The candidate inhibitors (compounds 4, 5, 7-9 and 11-13) were tested against Zanamivir and/or 2,3-DFSA for their cytopathic effect (CPE) against two influenza A strains. It is important to note that, although the present Cytoprotection assay is a good qualitative indicator of antiviral activity, the results are often variable (as note by others, Tisdale M. (2000)) and caution exercised when doing a quantitative analysis.







TABLE 5







The activity of compounds against influenza A/Brisbane/10/2007 (H3N2)











Replicate 1
Replicate 2
Toxicity














Cytopro-
Rela-
Cytopro-


Rela-



tective
tive
tective


tive



effect
ac-
effect
Relative

tox-


Compound
(ng/mL)
tivity
(ng/mL)
activity
(ug/mL)
icity
















Zanamivir
277.4
1.0
369.9
1.0
568
1.0


4 (DFSA-
6.9
40.0
9.2
40.2
3.6
160.0


4NH2)








5 (N3-
7.6
36.4
7.6
48.6
125.0
4.5


105ET)








7 (N3-
122
2.3
61.0
6.1
7.8
72.7


102ET)








12 (DFSA-
55.5
5.0
74
5.0
0.9
640.0


4Gu)








13 (N3-
44.4
6.3
44.4
8.3
1.4
400.0


106ET)








11 (N3-
1598
0.2
—
—
102.3
5.6


107AC)








8 (N3-
104.3
2.7
—
—
53.4
10.6


109N3)








9 (N3-
117.6
2.4
—
—
7.5
75.5


111AMD)
















TABLE 6







The activity of compounds against


influenza A/Denver/1/57 (H1N1)









Compound
Cytoprotective effect (ng/mL)
Relative activity












Zanamivir
4.3
1.0


4 (DFSA-4NH2)
887.8
0.005


7 (N3-102ET)
3906.3
0.001


5 (N3-105ET)
488.3
0.009


12 (DFSA-4Gu)
887.8
0.005


13 (N3-106ET)
710.2
0.006


9 (N3-111AMD)
3764.2
0.001


23DFSA
4261.4
0.001
















TABLE 7







The activity of compounds 4 and 12 against a variety of influenza


A viruses and an Oseltamivir resistant strain (OsR)









Antiviral compound














DFSA-4NH2
DFSA-4Gu


Virus

Zanamivir
(compound 4)
(compound 12)


(100 × TCID50)
Trials
[μM]
[μM]
[μM]














A/Brisbane NML
1
0.834B
51.415B
12.215B


(H3N2)


A/Hong Kong
1
20.026B
1028.306B
73.288B


(H3N2)
2
5.007B
64.269B
4.581B



3
6.413B
>115.519C
42.183C


A/Denver (H1N1)
1
<0.209A
<12.854A
<3.054A


A/New Caledonia
1
0.417A
<12.854A
<3.054A


(H1N1)


A/Brisbane NML
1
6.675A
102.831A
6.107A


(H1N1)
2
8.344B
411.323B
3.054B



3
2.138B
>115.519C
4.474C


A/Brisbane 6770
1
<0.209A
12.854A
<3.054A


(HIN1-OsR)
2
0.039B
9.640B
0.382B



3
0.013B
2.410B
0.382B



4
0.025B
3.971C
0.300C





Multiple experiments were performed on different days on some strains


ASingle measurement;


BMean of dual measurements;


CMean of six measurements






TABLE 7 shows the concentration of antiviral (μM) at which a confluent monolayer of MDCK cells was protected over a 5 day period from cytopathic viral infection.
Example 4
Cytoprotection Assays with Influenza B Strains
The candidate inhibitors were tested against Zanamivir for their cytopathic effect (CPE) against two influenza B strains. It is important to note that, although the present Cytoprotection assay is a good qualitative indicator of antiviral activity, the results are often variable (as noted by others, Tisdale M. (2000)) and caution exercised when doing a quantitative analysis.







TABLE 8







The activity of compounds against influenza B/HongKong/5/72









Compound
Cytoprotective effect (ng/mL)
Relative activity












Zanamivir
2219
1.0


4 (DFSA-4NH2)
1775.6
1.25


7 (N3-102ET)
3906.3
0.57


5 (N3-105ET)
976.6
2.27


12 (DFSA-4Gu)
1775.6
1.25


13 (N3-106ET)
710.2
3.13


9 (N3-111AMD)
15056.8
0.15


23DFSA
34090.9
0.07
















TABLE 9







The activity of compounds against influenza B/Florida/04/06










Replicate 1
Replicate 2












Cytoprotective
Relative
Cytoprotective
Relative


Compound
effect (ng/mL)
activity
effect (ng/mL)
activity














Zanamivir
2219
1.0
887
1.0


4 (DFSA-4NH2)
947
2.3
1074
0.83


7 (N3-102ET)
3267
0.67
3267
0.27


5 (N3-105ET)
290
7.7
290
3.1









Example 5
In Vivo Pharmacokinetic (PK) Profile Study
The purpose of the study was to evaluate the pharmacokinetic profile of a novel fluorosialic compound (DFSA-4Gu), in comparison with Zanamivir by the intranasal route and to compare the pharmacokinetic profile of DFSA-4Gu when dosed by the intranasal and intravenous routes in the Balb/C mouse (Mus musculus).







TABLE 10







Study Parameters




















N per



Gp
Group
# of
Dose

Dose Volume
time



#
Name
Animals
(mg/kg)
Admin. Route
(μL)
point
Time-point

















1
DFSA-IV
23
1
Intravenous
200/20 g
4
5, 15, 30 min,







(10 μL/g)

1, 2 hr








3
7 d


2
DFSA-IN
23
1
Intranasal
10 μL/nostril/
4
5, 15, 30 min,







15 g

1, 2 hr







(0.67 μL/
3
7d







nostril/g)




3
ZAN-IN
23
1
Intranasal
10 μL/nostril/
4
5, 15, 30 min, 







15 g

1, 2 hr







(0.67 μL/
3
7 d







nostril/g)









A PK study was conducted to evaluate DFSA-4Gu in mice, administered by IN and IV routes, and to evaluate Zanamivir administered by the IN route, all at 1 mg/kg in a single dose. Tissue levels and plasma levels for DFSA-4Gu were obtained and lung tissue levels for Zanamivir were obtained. Levels declined with apparent first order kinetics in all tissues by both routes. Comparison of DFSA-4Gu and Zanamivir by the IN routes showed that comparable compound exposure in the lung was observed with both agents.
Calculated PK Parameters
PK parameters calculated for DFSA-4Gu and Zanamivir after intranasal dosing with a single 1 mg/kg dose.







TABLE 11A







Calculated PK Values















AUC
T1/2
Cinitial



Tissue
Compound
(ng × min/L)
(min)
(ng/mL)

















Plasma
DFSA-4Gu
76433
17.3
4954



Trachea
DFSA-4Gu
421865
28.1



Lung
DFSA-4Gu
236814
22.2



Lung
Zanamivir
254089
19.1










PK parameters calculated for DFSA-4Gu after intravenous dosing with a single 1 mg/kg dose.








TABLE 11B







Calculated PK Values















AUC
T1/2
Cinitial



Tissue
Compound
(ng × min/L)
(min)
(ng/mL)

















Plasma
DFSA-4Gu
88202
8.3
9480



Trachea
DFSA-4Gu
24749
9.4



Lung
DFSA-4Gu
25307
14.2










The apparent half-life for the compounds was longer after intranasal dosing than after intravenous dosing. As well the exposure, expressed as AUC for DFSA-4Gu was increased in trachea and lung by intranasal dosing, relative to intravenous dosing with AUC ratios (IN/IV) for lung=9.3 and for trachea=17.0.
The AUC in lung after intranasal dosing of Zanamivir and DFSA-4Gu were similar, AUC(Lung) (Zanamivir/DFSA-4Gu)=1.07. The apparent half-lives of the two agents in the lung after intranasal dosing were also similar.
Example 6
In Vivo Murine Model of Influenza Infection
The in vivo efficacy of Zanamivir, DFSA-4Gu & DFSA-4NH2 was tested in a mouse model of influenza (HK1). Compounds were compared to Zanamavir, whereby animals received a single intranasal dose of drug prior to infection and were given follow-up doses twice per day for a total of six days. Mortality due to infection was set at 15% weight loss after which animals were euthanized. Survival of animals was significantly extended in groups that received a 1 mg/Kg dose of DFSA-4GU and Zanamavir, as compared to the untreated control group. Similarly, treatment with DFSA-4NH2 did somewhat extend survival as compared to the untreated control group. However, drug treatments did not prevent mortality as all treated animals eventually reached endpoint due to influenza infection. Although these results are promising, the use of a different virus strain or an increased dose of drugs may alter the survival.







TABLE 12







Experimental Groups and Treatments












Gp

Test

Doses
Virus


#
Group Name
Article
N
(mg/kg/d)
Strain















1
PR8-DFSA-4Gu
DFSA-4Gu
10
1
A/Hong


2
PR8-DFSA-4NH2
DFSA-4NH2
10
1
Kong/1/68


3
PR8-ZAN
Zanamivir
10
0.1
(H3N2)


4
PR8-ZAN
Zanamivir
10
1


5
PR8-Untreated
Saline
10
N/A


6
DFSA-4NH2-Control
DFSA-4NH2
 5†
1
None


7
DFSA-4Gu-Control
DFSA-4Gu
 5
1










Total N:
60





†The original protocol called for N = 3 for each healthy control group (Groups 6 and 7 in Table 6). In the execution of the study, N = 5 were used in these groups, increasing the total animal number to 60.













TABLE 13







Survival rates of animals on days 3, 4, 5, 6, 7 and 8 post infection.


TABLE 13 shows the number of animals remaining in each group. 


N = 10 for all groups except groups 6 and 7 (n = 5)



















Day 3
Day 4
Day 4
Day 5
Day 5
Day 6
Day 6
Day 7
Day 7
Day 8
Day 8



pm
am
pm
am
pm
am
pm
am
pm
am
pm





Group 1 HK1-
10
10
8
7
6
5
4
3
1
1
0


DFSA-Gu-













(1 mg/kg)













Group 2 HK1-
10
10
3
2
1
0
0
0
0
0
0


DFSA-4NH2-













(1 mg/kg)













Group 3 HK1-
10
 9
2
1
1
0
0
0
0
0
0


ZAN-(0.1 mg/kg)













Group 4-HK1-
10
10
9
5
3
2
1
0
0
0
0


ZAN-(1 mg/kg) 













Group 5
10
10
4
1
0
0
0
0
0
0
0


Untreated













Group 6 DFSA- 
 5
 5
5
5
5
5
5
5
5
5
5


4NH2-Control













Group 7 DFSA-
 5
 5
5
5
5
5
5
5
5
5
5


4GU-Control
















TABLE 14







Mean % weight loss per group on day 4 (am) post infection. This


time point was selected because many animals were on the verge


of reaching endpoint, but most groups still had 10 animals.










P-
% weight loss



value1
mean +/− SD















Group 1 HKl-DFSA-4Gu-1.0
0.01
10.75 +/− 4.87



Group 2 HK1-DFSA-4NH2-1.0
0.14
16.02 +/− 2.07



Group 3 HK1-ZAN-0.1
0.06
16.73 +/− 2.62



Group 4-HK1-ZAN-1.0
0.01
12.24 +− 2.34



Group 6 DFSA-4NH2-Control
0.00
 2.68 +/− 1.76



Group 7 DFSA-4GU-Control
0.00
 4.72 +/− 2.78



Group 5 Untreated

  15 +/− 2.06







1P-value determined by student's T-test in which each group was individually compared to the mean of untreated group #7. A p-value below 0.05 was considered statistically significant.






With regards to the above results, and the potential alteration in protocol, the use of a different virus strain, a different end point, or an increased dose of drugs may be justified. For example, another mouse adapted virus strain such as A/PR/8/34 may produce different results. Also, an endpoint of 15% weight loss may be too low, whereby animals may still recover after losing 20% or more. For example Bantia et al. (2001) reported recovery of Zanamavir treated animals after more than 20 percent weight loss. Similarly, a higher dose of drug may be required to achieve complete protection. For example, Levena et al. (2001), the authors required doses of Zanamavir at 10 and 50 mg/kg/day before they observed a protective effect.
Fluorinated compounds of the class described in this invention are inhibitors of a range of glycosidases, and specific with respect to their target enzymes. These compounds are mechanism-based in their inhibitory action. They bind to the enzyme like the normal substrate and undergo the first step of catalysis (intermediate formation) like the natural substrate, but then only very slowly undergo the second step (turnover via hydrolysis). Importantly, this mechanism-basis inhibition should make resistance formation by viruses more difficult. Since the inhibitors are mechanism-based, any mutations in the viral enzyme that reduce the inhibition must necessarily reduce the efficiency of the enzyme on the natural substrate. FIG. 2 shows an example of neuraminidase inhibition by 3-fluorosialyl fluoride (1, 23DFSA) (note: sialic acid numbering is different from that of normal sugars due to the anomeric carboxylate).
The fluorosialics differ fundamentally from Zanamivir and Oseltamivir in two major ways. Zanamivir and Oseltamivir are reversibly binding inhibitors that interact with the enzyme active site very tightly due to their flattened, cyclic conformation. Their binding mode likely imitates the transition state conformation of the sugar during hydrolysis. They are therefore transition state mimics. The fluorosialics, by contrast, contain no double bond thus adopt a regular chair conformation. They react with the enzyme as if they are substrates and form a covalent bond with the active site nucleophile, and only hydrolyse to products very slowly. They derive their very high efficacies from the long-lived nature of the intermediate formed.
Due to this completely different mode of action it was not evident that incorporation of an amine or guanidine substituent would increase their effectiveness since the sugar ring has a very different conformation in the two cases: the amine/guanidine would be presented in a very different manner in the two cases, thus likely interacts quite differently. Even more importantly, the major efficacy of the fluorosialics derives from the formation of a relatively long-lived covalent intermediate. It was not at all evident that incorporation of the 4-amine/guanidine in place of the 4-hydroxyl would slow down the hydrolysis of this intermediate (deglycosylation) much more than it slowed the formation of the intermediate (glycosylation) with the result of a much longer-lived intermediate thus a higher efficacy inhibitor.
Although various embodiments of the invention are disclosed herein, many adaptations and modifications may be made within the scope of the invention in accordance with the common general knowledge of those skilled in this art. Such modifications include the substitution of known equivalents for any aspect of the invention in order to achieve the same result in substantially the same way. Numeric ranges are inclusive of the numbers defining the range. The word “comprising” is used herein as an open-ended term, substantially equivalent to the phrase “including, but not limited to”, and the word “comprises” has a corresponding meaning. As used herein, the singular forms “a”, “an” and “the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to “a thing” includes more than one such thing. Citation of references herein is not an admission that such references are prior art to the present invention.
References


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## Claims

1. A method comprising:
reacting a compound SAN3:




with 1-chloromethyl-4-fluoro-1,4-diazoniabicyclo[2.2.2]octane bis(tetrafluoroborate) in the presence of MeNO2/H2O for at least 4 days to form compound 1:





and
reacting compound 1 with diethylaminosulfur trifluoride (DAST) in CH2Cl2 at a temperature of −30° C. to 0° C. to form compound 2:

2. The method according to claim 1 further comprising:
mixing compound 2 with NaOMe and MeOH;
then mixing with Pd/C, H2, and MeOH; and
then mixing with LiOH, H2O, and MeOH to form compound 4:

3. The method according to claim 1 further comprising:
mixing compound 2 with NaOMe and MeOH;
then mixing with AcOH until neutral;
then mixing with PMe3, H2O, and MeOH; and
then reacting with compound VII:




in Et3N, MeOH, and DMF to produce compound VIII:




reacting compound VIII with LiOH, H2O, and THF; and
then reacting with Pd/C, H2, H2O, and THF to form compound 12: