Initial to indicate certification as to information on this page (full text of certification is on page one). Date INSTRUCTIONS: Please type or print in ink. Please read carefully the attached instructions and supply the information requested on this form. Use additional sheet(s) if necessary. 2. Case # and Name 3. Date and Type of DCI and Number

4. EPA Product Registration 5. I wish to cancel this product registration voluntarily 6. Generic Data 7. Product Specific Data 6a. I am claiming a Generic Data Exemption because I obtain the active ingredient from the source EPA registration number listed below. 6b. I agree to satisfy Generic Data Requirements as indicated on the attached form entitled "Requirements Status and Registrant's Response." 7a. My product is an MUP and I agree to satisfy the MUP requirement on the attached form entitled "Requirements Status and Registrant's Response." 7b. My product is an EUP and I agree to satisfy the EUP requirement on the attached form entitled "Requirements Status and Registrant's Response." OMB Approval 2070-0174

1. Company Name and Address 0288 - Diquat dibromide Chemical # and Name: 032201 Diquat dibromide 31-Mar-2011 GENERIC ID # GDCI-032201-824 

### United States Environmental Protection Agency

Washington, D.C. 20460 DATA CALL-IN RESPONSE 1 Page of 1

8. Certification: I certify that the statements made on this form and all attachments are true, accurate, and complete. I acknowledge that any knowingly false or misleading statement may be punishable by fine, imprisonment or both under applicable law. Signature and Title of Company's Authorized Representative ______________________________________________________ 10. Name of Company N/A N/A

9. Date
11. Phone NumberInitial to indicate certification as to information on this page (full text of certification is on page one) 13. Phone Number 10. Certification: I certify that the statements made on this form and all attachments are true, accurate, and complete. I acknowledge that any knowingly false or misleading statement may be punishable by fine, imprisonment or both under applicable law. 12. Name of Company 11. Date OMB Approval 2070-0174 

### United States Environmental Protection Agency

Washington, D.C. 20460 REQUIREMENTS STATUS AND REGISTRANT'S RESPONSE INSTRUCTIONS: Please type or print in ink. Please read carefully the attached instructions and supply the information requested on this form. Use additional sheet(s) if necessary.

4. Guideline Requirement Number 5. Study Title Progress Reports



### 2 3 1 6. Use

Pattern 7. Test Substance 8. Time Frame (Months) 9. Registrant

Response P R O T O C O L 1. Company Name and Address 2. Case # and Name 3. Date and Type of DCI and Number

Signature and Title of Company's Authorized Representative _________________________________________________________________

Environmental Fate Data Requirements (Conventional Chemical)

Nontarget Plant Protection Data Requirements (Conventional Chemical)

Terrestrial and Aquatic Nontarget Organisms Data Requirements (Conventional Chemical) 835.6100 835.6200

850.4150 850.4400 850.5400

850.1350 Terrestrial field dissipation

Aquatic field dissipation
Terrestrial plant toxicity, Tier 1 (vegetative vigor) Aquatic plant toxicity test using Lemna spp. Tiers I and II Algal toxicity, Tiers 1 and II
Mysid chronic toxicity test 0288 - Diquat dibromide Chemical # and Name: 032201 Diquat dibromide 31-Mar-2011 GENERIC ID # GDCI-032201-824

E,C,A,D,DD E,C,A,D,DD

E,C,A,D,DD E,C,A,D,DD E,C,A,D,DD

E,C,A,D,DD TEP TEP

TEP TEP or TGAI TEP or TGAI

TGAI 12 12

24 12 12

12 1 of Page

(6) (5)
(4, 13, 21) (15, 20) (14, 21)
(11) N N

N N N

N 2Date OMB Approval 2070-0174 

### United States Environmental Protection Agency

Washington, D.C. 20460 REQUIREMENTS STATUS AND REGISTRANT'S RESPONSE INSTRUCTIONS: Please type or print in ink. Please read carefully the attached instructions and supply the information requested on this form. Use additional sheet(s) if necessary.

4. Guideline Requirement Number 5. Study Title Progress Reports



### 2 3 1 6. Use

Pattern 7. Test Substance 8. Time Frame (Months) 9. Registrant

Response P R O T O C O L 1. Company Name and Address 2. Case # and Name 3. Date and Type of DCI and Number

Terrestrial and Aquatic Nontarget Organisms Data Requirements (Conventional Chemical)

Toxicology Data Requirements (Conventional Chemical) 850.1400 850.2100

870.7800

SS-1062 SS-1063 SS-1064 Fish early-life stage toxicity test

Avian acute oral toxicity test
Immunotoxicity
Whole Sediment Life Cycle Tox Test w/Estuarine Invertebrates Sediment Life Cycle Tox Test w/Freshwater Invertebrates Sediment Life Cycle Tox Test with Freshwater Invertebrates 0288 - Diquat dibromide Chemical # and Name: 032201 Diquat dibromide 31-Mar-2011 GENERIC ID # GDCI-032201-824

E,C,A,D,DD E,C,A,D,DD
E,C,A,D,DD
E,C,A,D,DD E,C,A,D,DD E,C,A,D,DD TGAI TGAI, TEP

TGAI 12 12

12

24 24 24 2 of Page

(12) (10, 19)
(3)
(1, 2, 8, 18) (1, 2, 7, 16) (1, 2, 9, 17) N

Y

Y

Y Y Y 2

### United States Environmental Protection Agency Washington, D.C. 20460 FOOTNOTES AND KEY DEFINITIONS FOR GUIDELINE REQUIREMENTS 0288 - Diquat dibromide GDCI-032201-824

1 2 3 4 5
6
7 8 9 10 11 12 13 Key: Residue of Concern = Residue of Concern; TEP = Typical End Use Product [TEP]; TGAI = Technical Grade Active Ingredient [TGAI]; TGAI/PAIRA = Technical Grade of the Active Ingredient or Pure Active Ingredient, Radio Labelled; TGAI, TEP = Technical Grade of the Active Ingredient or Technical End-Use Product; TGAI/PAI = Technical Grade Active Ingredient, Pure Active Ingredient

The draft protocol must be submitted to the Agency within 120 days of receipt of this DCI. Test substance should be Technical Grade Active Ingredient. Registrants may submit a protocol for review prior to study initiation. Refer to public draft guideline 850.4250 for guidance on Tier II vegetative vigor testing. Only the environmental chemistry method is required. Environmental chemistry methods must include results of a successful confirmatory method trial by an independent laboratory. The environmental chemistry methods must include a statement of no data confidentiality claims, i.e., non-CBI. Test standards and procedures for independent laboratory validation are available as addenda to the guideline for this test requirement. Only the environmental chemistry method is required. Environmental chemistry methods must include results of a successful confirmatory method trial by an independent laboratory. The environmental chemistry methods must include a statement of no data confidentiality claims, i.e., non-CBI. Test standards and procedures for independent laboratory validation are available as addenda to the guideline for this test requirement. For guidance to develop a study protocol refer to Test Method 100.5: Life-cycle Test for Measuring the Effects of sediment-associated Contaminants on Chironomus dilutus or C. tentans in USEPA 2000 Methods for Measuring the Toxicity and Bioaccumulation of Sediment-associated Contaminants with Freshwater Invertebrates EPA 600/R-99/064. For guidance on developing a protocol for this study refer to USEPA 2001 Method for Assessing the Chronic Toxicity of Marine and Estuarine Sediment-associated Contaminants with the Amphipod Leptocheirus plumulosus EPA 600/R-01/020. For guidance on developing a protocol for this study refer to Test Method 100.4: Hyalella azteca 42-d Test for Measuring the Effects of Sediment Associated Contaminants on Survival, Growth, and Reporduction from USEPA 2000 Methods for Measuring the Toxicity and Bioaccumulation of Sediment-associated Contaminants with Freshwater Invertebrates EPA 600/R-99/064. Data are required on one passerine species and either one waterfowl species or one upland game bird species for terrestrial, aquatic, forestry, and residential outdoor uses. Data are preferred on waterfowl or upland game bird species for indoor and greenhouse uses. Data are required on one estuarine/marine invertebrate species. Data are required on one estuarine/marine fish species. Data are required for six species of dicots from at least four families, one species of which is soybean (Glycine max). Data are required for four species of monocots from at least two families, one species which is corn (Zea mays). At least one of either the monocot or dicot species must be a root crop. Case # and Name: DCI Number: 1 Page: of

Footnotes: The following footnotes are referenced in column two (5. Study Title) of the Requirements Status and Registrant's Response form. These footnotes apply in addition to any test notes included in 40 CFR Part 158 with respect to the particular data requirement. Use Categories Key: A - Terrestrial food crop C - Terrestrial nonfood crop D - Aquatic food crop E - Aquatic nonfood outdoor use DD - Aquatic areas 2

### United States Environmental Protection Agency Washington, D.C. 20460 FOOTNOTES AND KEY DEFINITIONS FOR GUIDELINE REQUIREMENTS 0288 - Diquat dibromide GDCI-032201-824

14 15 16

17

18
19 20

21 Key: Residue of Concern = Residue of Concern; TEP = Typical End Use Product [TEP]; TGAI = Technical Grade Active Ingredient [TGAI]; TGAI/PAIRA = Technical Grade of the Active Ingredient or Pure Active Ingredient, Radio Labelled; TGAI, TEP = Technical Grade of the Active Ingredient or Technical End-Use Product; TGAI/PAI = Technical Grade Active Ingredient, Pure Active Ingredient

Data are required for a freshwater green alga species, a freshwater diatom species, a marine diatom species, and a cyanobacterium (formerly known as blue-green algae). Data are required for a duckweed species. A protocol should be submitted for approval prior to testing. The preferred sediment is formulated, guidance for the formulated sediment can be found in OECD 218 Sediment-Water Chironomid Toxicity Test using Spiked Sediment. Based on test variability for the endpoints of concern and the need to establish a NOAEC/LOAEC the following number of replicates and test animals are recommended: for the midge test with C. dilutus or C. tentans 16 replicates containing 12 test organisms each (12 replicates at test initiation; 4 added at 10 days for auxiliary males). For alternative test designs the protocol should discuss the power of the test. A protocol should be submitted for approval prior to testing. The preferred sediment is formulated, guidance for the formulated sediment can be found in OECD 218 Sediment-Water Chironomid Toxicity Test using Spiked Sediment. Based on test variability for the endpoints of concern and the need to establish a NOAEC/LOAEC the following number of replicates and test animals are recommended: for the freshwater amphipod H. azteca test of 12 replicates per treatment (4 for 28-d survival and growth and 8 for the remainder of the test) with 10 neonates per replicate. For alternative test designs the protocol should discuss the power of the test. A protocol should be submitted for approval prior to testing. The preferred sediment is formulated, guidance for the formulated sediment can be found in OECD 218 Sediment-Water Chironomid Toxicity Test using Spiked Sediment. Based on test variability for the endpoints of concern and the need to establish a NOAEC/LOAEC the following number of replicates and test animals are recommended: for the L. plumulosus 10 replicates per treatment with 20 neonates per replicate. For alternative test designs the protocol should discuss the power of the test. A protocol must be submitted for review prior to study initiation. A Tier II study is required. A Tier I plant study may be conducted in lieu of a Tier II study with the understanding that any adverse effects observed by the Tier I study would necessitate conduct and submission of a Tier II study as well. The purpose of a Tier II study is to establish both a definitive No Observed Adverse Effect Concentration (NOAEC), or alternatively, a concentration at which there is a 5% observed inhibition effect (IC05), to be used in a risk assessment and effects determination for endangered or threatened species (listed species), and a definitive IC50 (concentration at which there is a 50% observed inhibition effect) for assessing risk to non-listed nontarget plants. If any adverse effects are observed in a Tier I study and neither a definitive NOAEC nor a definitive IC05 value is available, then the Agency may have to presume in its effects determination, that diquat dibromide "may affect" and is "likely to adversely affect" listed plant species. A Tier II study is required. A Tier I plant study may be conducted in lieu of a Tier II study with the understanding that any adverse effects observed by the Tier I study would necessitate conduct and submission of a Tier II study as well. The purpose of a Tier II study is to establish both a definitive No Observed Adverse Effect Concentration (NOAEC), or alternatively, a concentration at which there is a 5% observed inhibition effect (IC05), to be used in a risk assessment and effects determination for endangered or threatened species (listed species), and a definitive IC25 (concentration at which there is a 25% observed inhibition effect) for assessing risk to non-listed nontarget plants. If any adverse effects are observed in a Tier I study and neither a definitive NOAEC nor a definitive IC05 value is available, then the Agency may have to presume in its effects determination, that diquat dibromide "may affect" and is "likely to adversely affect" listed plant species. Case # and Name: DCI Number: 2 Page: of 2

### United States Environmental Protection Agency Washington, D.C. 20460 LIST OF ALL REGISTRANTS SENT THIS DATA CALL-IN NOTICE 0288 - Diquat dibromide Case # and Name: 1 Page: of 1

35935 2749 100 82633 NUFARM LIMITED

ACETO AGRICULTURAL CHEMICALS CORP SYNGENTA CROP PROTECTION, LLC SHARDA WORLDWIDE EXPORTS PVT. LTD. Co. Nr. Company Name Agent For Company NUFARM LIMITED

WAGNER REGULATORY ASSOCIATES, INC. 4020 AERIAL CENTER PKWY., STE 103 4 TRI HARBOR COURT 410 SWING ROAD, P.O. Box 18300 P.O. Box 640 Address City ZIP MORRISVILLE PORT WASHINGTON GREENSBORO HOCKESSIN 27560 11050 274198300 19707 OMB Approval 2070-0174

State NC NY NC DE DCI Number: GDCI-032201-824

# LINKS TO FORMS: The pesticide registration forms that are listed below can be found at: http://www.epa.gov/opprd001/forms/ Link to C onfidential Statement of Formula and Instructions (Form # 8570 -4 

# ) http://www.epa.gov/opprd001/forms/8570 - 4.pdf C ertification of Attempt to Enter Into an Agreement With Registrants for Development of Data (Form # 8570 - 32 

# ) http://www.epa.gov/opprd001/forms/8570 - 32.pdf C ertification With Respect to Citation of Data (Form # 8570 - 34 

# ) http://www.epa.gov/opprd001/forms/8570 - 34.pdf