Initial to indicate certification as to information on this page

(full text of certification is on page one).

Date

INSTRUCTIONS: Please type or print in ink. Please read carefully the attached instructions and supply the information requested on this form. 

Use additional sheet(s) if necessary.

2. Case # and Name 3. Date and Type of DCI and Number

4. EPA Product 

Registration

5. I wish to cancel 

this product 

registration 

voluntarily

6. Generic Data 7. Product Specific Data

6a. I am claiming a Generic Data 

Exemption because I obtain the 

active ingredient from the source 

EPA registration number listed 

below.

6b. I agree to satisfy Generic Data

Requirements as indicated on the 

attached form entitled 

"Requirements Status and 

Registrant's Response."

7a. My product is an MUP and I 

agree to satisfy the MUP 

requirement on the attached form

entitled "Requirements Status and

Registrant's Response."

7b. My product is an EUP and I 

agree to satisfy the EUP 

requirement on the attached form 

entitled "Requirements Status and 

Registrant's Response."

OMB Approval 2070-0174

1. Company Name and Address

 

0288 - Diquat dibromide

Chemical # and Name: 032201

Diquat dibromide

31-Mar-2011

GENERIC

ID # GDCI-032201-824

United States Environmental Protection Agency

Washington, D.C. 20460

DATA CALL-IN RESPONSE

Page of 1 1

8. Certification: I certify that the statements made on this form and all attachments are true, accurate, and complete. I acknowledge that any

knowingly false or misleading statement may be punishable by fine, imprisonment or both under applicable law.

Signature and Title of Company's Authorized Representative ______________________________________________________

10. Name of Company

N/A N/A

9. Date

11. Phone Number 
Initial to indicate certification as to information on this page (full text of certification is on page one) 

13. Phone Number

10. Certification: I certify that the statements made on this form and all attachments are true, accurate, and complete. I acknowledge that any 

knowingly false or misleading statement may be punishable by fine, imprisonment or both under applicable law.

12. Name of Company

11. Date

OMB Approval 2070-0174

United States Environmental Protection Agency 

Washington, D.C. 20460

REQUIREMENTS STATUS AND REGISTRANT'S RESPONSE

INSTRUCTIONS: Please type or print in ink. Please read carefully the attached instructions and supply the information requested on this form. Use additional sheet(s) if necessary.

4. Guideline

Requirement

Number

5. Study Title Progress 

Reports

1 2 3

6. Use 

Pattern

7. Test 

Substance

8. Time

Frame 

(Months)

9. Registrant

Response

P

R

O

T

O

C

O

L

1. Company Name and Address 2. Case # and Name 3. Date and Type of DCI and Number

Signature and Title of Company's Authorized Representative _________________________________________________________________

 

Environmental Fate Data Requirements (Conventional 

Chemical)

Nontarget Plant Protection Data Requirements (Conventional 

Chemical)

Terrestrial and Aquatic Nontarget Organisms Data 

Requirements (Conventional Chemical)

835.6100

835.6200

850.4150

850.4400

850.5400

850.1350

Terrestrial field dissipation

Aquatic field dissipation

Terrestrial plant toxicity, Tier 1 (vegetative vigor)

Aquatic plant toxicity test using Lemna spp. Tiers I 

and II

Algal toxicity, Tiers 1 and II

Mysid chronic toxicity test

0288 - Diquat dibromide

Chemical # and Name: 032201

Diquat dibromide

31-Mar-2011

GENERIC

ID # GDCI-032201-824

E,C,A,D,DD

E,C,A,D,DD

E,C,A,D,DD

E,C,A,D,DD

E,C,A,D,DD

E,C,A,D,DD

TEP

TEP

TEP

TEP or TGAI

TEP or TGAI

TGAI

12

12

24

12

12

12

Page 1 of 

(6)

(5)

(4, 13, 21)

(15, 20)

(14, 21)

(11)

N

N

N

N

N

N

2
Date

OMB Approval 2070-0174

United States Environmental Protection Agency 

Washington, D.C. 20460

REQUIREMENTS STATUS AND REGISTRANT'S RESPONSE

INSTRUCTIONS: Please type or print in ink. Please read carefully the attached instructions and supply the information requested on this form. Use additional sheet(s) if necessary.

4. Guideline

Requirement

Number

5. Study Title Progress 

Reports

1 2 3

6. Use 

Pattern

7. Test 

Substance

8. Time

Frame 

(Months)

9. Registrant

Response

P

R

O

T

O

C

O

L

1. Company Name and Address 2. Case # and Name 3. Date and Type of DCI and Number

 

Terrestrial and Aquatic Nontarget Organisms Data 

Requirements (Conventional Chemical)

Toxicology Data Requirements (Conventional Chemical)

850.1400

850.2100

870.7800

SS-1062

SS-1063

SS-1064

Fish early-life stage toxicity test

Avian acute oral toxicity test

Immunotoxicity

Whole Sediment Life Cycle Tox Test w/Estuarine 

Invertebrates

Sediment Life Cycle Tox Test w/Freshwater 

Invertebrates

Sediment Life Cycle Tox Test with Freshwater 

Invertebrates

0288 - Diquat dibromide

Chemical # and Name: 032201

Diquat dibromide

31-Mar-2011

GENERIC

ID # GDCI-032201-824

E,C,A,D,DD

E,C,A,D,DD

E,C,A,D,DD

E,C,A,D,DD

E,C,A,D,DD

E,C,A,D,DD

TGAI

TGAI, TEP

TGAI

12

12

12

24

24

24

Page 2 of 

(12)

(10, 19)

(3)

(1, 2, 8, 18)

(1, 2, 7, 16)

(1, 2, 9, 17)

N

Y

Y

Y

Y

Y

2
United States Environmental Protection Agency 

Washington, D.C. 20460

FOOTNOTES AND KEY DEFINITIONS FOR GUIDELINE REQUIREMENTS

0288 - Diquat dibromide

GDCI-032201-824

1

2

3

4

5

6

7

8

9

10

11

12

13

Key: Residue of Concern = Residue of Concern; TEP = Typical End Use Product [TEP]; TGAI = Technical Grade Active Ingredient [TGAI]; TGAI/PAIRA = Technical Grade of the Active Ingredient or Pure Active 

Ingredient, Radio Labelled; TGAI, TEP = Technical Grade of the Active Ingredient or Technical End-Use Product; TGAI/PAI = Technical Grade Active Ingredient, Pure Active Ingredient

The draft protocol must be submitted to the Agency within 120 days of receipt of this DCI.

Test substance should be Technical Grade Active Ingredient.

Registrants may submit a protocol for review prior to study initiation.

Refer to public draft guideline 850.4250 for guidance on Tier II vegetative vigor testing.

Only the environmental chemistry method is required. Environmental chemistry methods must include results of a successful confirmatory method trial by an independent laboratory. The 

environmental chemistry methods must include a statement of no data confidentiality claims, i.e., non-CBI. Test standards and procedures for independent laboratory validation are available as 

addenda to the guideline for this test requirement.

Only the environmental chemistry method is required. Environmental chemistry methods must include results of a successful confirmatory method trial by an independent laboratory. The 

environmental chemistry methods must include a statement of no data confidentiality claims, i.e., non-CBI. Test standards and procedures for independent laboratory validation are available as 

addenda to the guideline for this test requirement.

For guidance to develop a study protocol refer to Test Method 100.5: Life-cycle Test for Measuring the Effects of sediment-associated Contaminants on Chironomus dilutus or C. tentans in 

USEPA 2000 Methods for Measuring the Toxicity and Bioaccumulation of Sediment-associated Contaminants with Freshwater Invertebrates EPA 600/R-99/064.

For guidance on developing a protocol for this study refer to USEPA 2001 Method for Assessing the Chronic Toxicity of Marine and Estuarine Sediment-associated Contaminants with the 

Amphipod Leptocheirus plumulosus EPA 600/R-01/020.

For guidance on developing a protocol for this study refer to Test Method 100.4: Hyalella azteca 42-d Test for Measuring the Effects of Sediment Associated Contaminants on Survival, Growth, 

and Reporduction from USEPA 2000 Methods for Measuring the Toxicity and Bioaccumulation of Sediment-associated Contaminants with Freshwater Invertebrates EPA 600/R-99/064. 

Data are required on one passerine species and either one waterfowl species or one upland game bird species for terrestrial, aquatic, forestry, and residential outdoor uses. Data are 

preferred on waterfowl or upland game bird species for indoor and greenhouse uses.

Data are required on one estuarine/marine invertebrate species.

Data are required on one estuarine/marine fish species.

Data are required for six species of dicots from at least four families, one species of which is soybean (Glycine max). Data are required for four species of monocots from at least two families,

one species which is corn (Zea mays). At least one of either the monocot or dicot species must be a root crop.

Case # and Name:

DCI Number:

Page: 1 of

Footnotes: The following footnotes are referenced in column two (5. Study Title) of the Requirements Status and Registrant's Response form. 

These footnotes apply in addition to any test notes included in 40 CFR Part 158 with respect to the particular data requirement.

Use Categories Key:

A - Terrestrial food crop

C - Terrestrial nonfood crop

D - Aquatic food crop

E - Aquatic nonfood outdoor use

DD - Aquatic areas

2
United States Environmental Protection Agency 

Washington, D.C. 20460

FOOTNOTES AND KEY DEFINITIONS FOR GUIDELINE REQUIREMENTS

0288 - Diquat dibromide

GDCI-032201-824

14

15

16

17

18

19

20

21

Key: Residue of Concern = Residue of Concern; TEP = Typical End Use Product [TEP]; TGAI = Technical Grade Active Ingredient [TGAI]; TGAI/PAIRA = Technical Grade of the Active Ingredient or Pure Active 

Ingredient, Radio Labelled; TGAI, TEP = Technical Grade of the Active Ingredient or Technical End-Use Product; TGAI/PAI = Technical Grade Active Ingredient, Pure Active Ingredient

Data are required for a freshwater green alga species, a freshwater diatom species, a marine diatom species, and a cyanobacterium (formerly known as blue-green algae).

Data are required for a duckweed species.

A protocol should be submitted for approval prior to testing. The preferred sediment is formulated, guidance for the formulated sediment can be found in OECD 218 Sediment-Water Chironomid 

Toxicity Test using Spiked Sediment. Based on test variability for the endpoints of concern and the need to establish a NOAEC/LOAEC the following number of replicates and test animals are 

recommended: for the midge test with C. dilutus or C. tentans 16 replicates containing 12 test organisms each (12 replicates at test initiation; 4 added at 10 days for auxiliary males). For 

alternative test designs the protocol should discuss the power of the test.

A protocol should be submitted for approval prior to testing. The preferred sediment is formulated, guidance for the formulated sediment can be found in OECD 218 Sediment-Water Chironomid 

Toxicity Test using Spiked Sediment. Based on test variability for the endpoints of concern and the need to establish a NOAEC/LOAEC the following number of replicates and test animals are 

recommended: for the freshwater amphipod H. azteca test of 12 replicates per treatment (4 for 28-d survival and growth and 8 for the remainder of the test) with 10 neonates per replicate. For

alternative test designs the protocol should discuss the power of the test.

A protocol should be submitted for approval prior to testing. The preferred sediment is formulated, guidance for the formulated sediment can be found in OECD 218 Sediment-Water Chironomid 

Toxicity Test using Spiked Sediment. Based on test variability for the endpoints of concern and the need to establish a NOAEC/LOAEC the following number of replicates and test animals are 

recommended: for the L. plumulosus 10 replicates per treatment with 20 neonates per replicate. For alternative test designs the protocol should discuss the power of the test.

A protocol must be submitted for review prior to study initiation.

A Tier II study is required. A Tier I plant study may be conducted in lieu of a Tier II study with the understanding that any adverse effects observed by the Tier I study would necessitate conduct

and submission of a Tier II study as well. The purpose of a Tier II study is to establish both a definitive No Observed Adverse Effect Concentration (NOAEC), or alternatively, a concentration at 

which there is a 5% observed inhibition effect (IC05), to be used in a risk assessment and effects determination for endangered or threatened species (listed species), and a definitive IC50 

(concentration at which there is a 50% observed inhibition effect) for assessing risk to non-listed nontarget plants. If any adverse effects are observed in a Tier I study and neither a definitive 

NOAEC nor a definitive IC05 value is available, then the Agency may have to presume in its effects determination, that diquat dibromide "may affect" and is "likely to adversely affect" listed plant 

species.

A Tier II study is required. A Tier I plant study may be conducted in lieu of a Tier II study with the understanding that any adverse effects observed by the Tier I study would necessitate conduct

and submission of a Tier II study as well. The purpose of a Tier II study is to establish both a definitive No Observed Adverse Effect Concentration (NOAEC), or alternatively, a concentration at 

which there is a 5% observed inhibition effect (IC05), to be used in a risk assessment and effects determination for endangered or threatened species (listed species), and a definitive IC25 

(concentration at which there is a 25% observed inhibition effect) for assessing risk to non-listed nontarget plants. If any adverse effects are observed in a Tier I study and neither a definitive 

NOAEC nor a definitive IC05 value is available, then the Agency may have to presume in its effects determination, that diquat dibromide "may affect" and is "likely to adversely affect" listed plant 

species.

Case # and Name:

DCI Number:

Page: 2 of 2
United States Environmental Protection Agency 

Washington, D.C. 20460

LIST OF ALL REGISTRANTS SENT THIS DATA CALL-IN NOTICE

Case # and Name: 0288 - Diquat dibromide

Page: 1 of 1

35935

2749

100

82633

NUFARM LIMITED

ACETO AGRICULTURAL CHEMICALS CORP

SYNGENTA CROP PROTECTION, LLC

SHARDA WORLDWIDE EXPORTS PVT. LTD.

Co. Nr. Company Name Agent For Company

NUFARM LIMITED

WAGNER REGULATORY 

ASSOCIATES, INC.

4020 AERIAL CENTER PKWY., STE 103

4 TRI HARBOR COURT

410 SWING ROAD, P.O. Box 18300

P.O. Box 640

Address City ZIP

MORRISVILLE

PORT WASHINGTON

GREENSBORO

HOCKESSIN

27560 

11050 

274198300

19707 

OMB Approval 2070-0174

State

NC

NY

NC

DE

 DCI Number: GDCI-032201-824
LINKS TO FORMS:

The pesticide registration forms that are listed below can be found at:

http://www.epa.gov/opprd001/forms/

Link to Confidential Statement of Formula and Instructions (Form # 8570-4)

http://www.epa.gov/opprd001/forms/8570-4.pdf

Certification of Attempt to Enter Into an Agreement With Registrants for 

Development of Data (Form # 8570-32)

http://www.epa.gov/opprd001/forms/8570-32.pdf

Certification With Respect to Citation of Data (Form # 8570-34)

http://www.epa.gov/opprd001/forms/8570-34.pdf