Exhibit 10.25


                      SECOND MODIFICATION OF LOAN AGREEMENT


THIS SECOND MODIFICATION is made as of this 10th day of December, 1999, by
and between IMMUCOR, INC., a Georgia corporation ("Borrower"), and WACHOVIA
BANK, N.A., a national banking association ("Lender").

                               Statement of Facts

                  Lender  and   Borrower   are  parties  to  that  certain  Loan
Agreement, dated as of October 27, 1998, as modified and amended by that certain
First  Modification  of Loan  Agreement,  dated as of April 30,  1999 (the "Loan
Agreement"),  pursuant to which Lender has agreed to make one or more loans from
time to time to the  Borrower  in  accordance  with  the  terms  and  conditions
thereof.  Lender and  Borrower  desire to modify the Loan  Agreement  in certain
respects,  all in accordance  with and subject to the terms and  conditions  set
forth herein.

                  NOW,  THEREFORE,   in  consideration  of  the  premises,   the
covenants  and  agreements   contained  herein,  and  other  good  and  valuable
consideration,  the  receipt  and  adequacy  of which are  hereby  acknowledged,
Borrower and Lender do hereby agree that all capitalized terms used herein shall
have the meanings  ascribed  thereto in the Loan Agreement  (except as otherwise
expressly defined or limited herein) and do hereby further agree as follows:

                               Statement of Terms

1. Amendments of Loan Agreement. Subject to the fulfillment of the
conditions precedent to the effectiveness of this Second Modification which
are set forth below, the Loan Agreement shall be amended from and after
this date as follows:

 (a)      The Loan Agreement is hereby  amended by adding each of the following
  definitions to Section 1.1 thereof in the appropriate alphabetical order:


     "Second  Additional  Term Loan"  shall mean the term loan in the  principal
     amount  of  $4,000,000  to be made by Lender to  Borrower  pursuant  to the
     provisions of Section 2.1.4.

     "Second  Additional Term Note" shall mean this term promissory  note, dated
     of even date herewith, as amended or supplemented from time to time, in the
     stated principal amount equal to the committed amount of Second  Additional
     Term Loan, together with any renewals or extensions thereof, in whole or in
     part.

"Second Modification Effective Date" shall mean the
date on which all of the conditions precedent to the effectiveness of
that certain Second Modification to Loan Agreement, dated as of
December 10, 1999, between the Borrower and the Lender, have been
satisfied.

(b) The Loan Agreement is hereby amended by amending each of the following
definitions in Section 1.1 to read as follows:


                           "Loans"  shall  mean the  Advances  under the Line of
                  Credit   together  with  the  Acquisition   Term  Loans,   the
                  Additional Term Loans and the Second Additional Term Loan.

                           "Notes"  shall mean,  collectively,  the Master Note,
                  the  Acquisition  Term Note, the Additional  Term Note and the
                  Second Additional Term Note.

                           "Term Loans" shall mean the  Acquisition  Term Loans,
                  the Additional Term Loans and the Second Additional Term Loan.

(c) The Loan Agreement is hereby amended by adding the following Section 2.1.4
thereto:

                           2.1.4  Second  Additional  Term Loans.  On the Second
         Modification Effective Date, and subject to the terms and conditions of
         this Agreement,  Lender agrees to make the Second  Additional Term Loan
         to Borrower,  the proceeds of which shall be used by Borrower solely to
         finance the  repayment  of the  outstanding  principal  amount of those
         certain three seller notes,  each dated December 11, 1996,  executed by
         3000524 Nova Scotia  Limited,  a Canadian  commercial  corporation  and
         subsidiary of the Borrower, in favor of Nubio Technologies Corporation,
         Patrick Waddy and Blaire MacNeil,  respectively, in connection with the
         acquisition  of  all of  the  capital  stock  of  Dominion  Biologicals
         Limited,  a  Canadian  corporation,  pursuant  to  that  certain  Share
         Purchase Agreement,  dated as of December 11, 1996, among such parties,
         and all accrued and unpaid interest thereon.  The Debt arising from the
         making of the Second  Additional  Term Loan shall be  evidenced  by the
         Second  Additional  Term Note,  which shall be executed by Borrower and
         delivered  to Lender on the Second  Modification  Effective  Date.  The
         principal amount of the Second  Additional Term Loan shall be repaid by
         the Borrower in installments as provided in the Second  Additional Term
         Note. In any event on December 1, 2002, the unpaid principal balance of
         the Second  Additional  Term Loan  together with all accrued but unpaid
         interest  thereon  shall  be  due  and  payable  in  full.  The  Second
         Additional  Term Loan  shall  bear  interest  at the  Applicable  Rate,
         calculated and payable in the manner  described in Section 2.2.1,  from
         the date thereof on the unpaid  principal  amount  thereof from time to
         time outstanding.  The Second  Additional Term Loan may be prepaid,  in
         whole  or in  part,  by  Borrower  at any  time  or  from  time to time
         hereafter; provided, however, that any partial prepayment of the Second
         Additional Term Loan shall be applied by Lender in the inverse order of
         the maturities of the principal  installments of the Second  Additional
         Term Loan then remaining to be paid.

(d) The Loan Agreement is hereby amended by amending clauses (ii) and (v)
of Section 2.2.1(c) to read as follows:


                           "(ii) no more than three (3) LIBOR  Borrowings  under
         each of the Line of Credit,  the Acquisition Term Loans, the Additional
         Term Loans and the Second  Additional Term Loans may be obtained at any
         time;

                           "(v) the Interest  Periods for, and aggregate  amount
         of, LIBOR  Borrowings  in respect of the  Acquisition  Term Loans,  the
         Additional  Term  Loans or the  Second  Additional  Term  Loans must be
         consistent with, and not exceed, the scheduled  principal  amortization
         thereof;"

(e) The Loan Agreement is hereby amended by amending the last sentence of
Section 2.4 to read as follows:

                                    "Any  prepayment made by Borrower under this
                  Section 2.4 shall be applied,  first,  to prepay the scheduled
                  installments of the Acquisition Term Loans in inverse order of
                  maturity  until  such Loan  shall  have been  prepaid in full,
                  second, to prepay the scheduled installments of the Additional
                  Term Loans in inverse order of maturity  until such Loans have
                  been  prepaid  in full  and  third  to  prepay  the  scheduled
                  installments  of the  Second  Additional  Term Loan in inverse
                  order of maturity until Loans have been prepaid in full."

(f) The Loan Agreement is hereby amended by deleting Schedule 3.4 thereof in its
entireties and by substituting in lieu thereof the following new Schedule 3.4
attached hereto and incorporated herein and therein by reference.

                  2. No Other  Amendments.  Except for the amendments  expressly
set forth and referred to in Sections 1 above,  the Loan Agreement  shall remain
unchanged and in full force and effect.  Nothing in this Second  Modification is
intended,  or shall be  construed,  to  constitute  a novation  or an accord and
satisfaction of any of the Borrower's  indebtedness or other indebtedness to the
Lender  under  or in  connection  with  the Loan  Agreement  (collectively,  the
"Obligations")  or to modify,  affect or impair the  perfection or continuity of
Lender's  security  interests  in,  security  titles  to or  other  liens on any
collateral for the Obligations.

                  3.  Representations and Warranties.  To induce Lender to enter
into this Second Modification,  the Borrower does hereby warrant,  represent and
covenant to Lender that: (a) each representation or warranty of the Borrower set
forth in the Loan  Agreement  is  hereby  restated  and  reaffirmed  as true and
correct on and as of the date hereof as if such  representation or warranty were
made  on and  as of  the  date  hereof  (except  to the  extent  that  any  such
representation  or  warranty  expressly  relates  to a  prior  specific  date or
period), and no Default or Event of Default has occurred and is continuing as of
this date under the Loan Agreement as amended by this Second  Modification;  and
(b) Borrower  has the power and is duly  authorized  to enter into,  deliver and
perform  this Second  Modification  and this Second  Modification  is the legal,
valid and binding  obligation of Borrower  enforceable  against it in accordance
with its terms.

                  4.  Conditions  Precedent  to  Effectiveness  of  this  Second
Modification.  The effectiveness of this Second  Modification and the amendments
provided  herein are subject to the truth and accuracy in all material  respects
of the  representations  and  warranties of the Borrower  contained in Section 3
above and to the fulfillment of the following additional  conditions  precedent:
(a)  Lender  shall  have  received  one or  more  counterparts  of  this  Second
Modification  duly  executed and  delivered by the  Borrower;  (b) if and to the
extent required by Lender,  any and all guarantors of the Obligations shall have
consented to the execution, delivery and performance of this Second Modification
and  all of  the  transactions  contemplated  hereby  by  signing  one  or  more
counterparts  of this Second  Modification  in the  appropriate  space indicated
below and  returning  same to  Lender;  (c) Lender  shall have  received a fully
earned,  non-refundable  additional loan origination fee of $20,000;  (d) Lender
shall have  received the Second  Additional  Term Note,  dated as of the date of
this  Second  Modification  and  having  a  stated  principal  amount  equal  to
$4,000,000,  duly executed and  delivered,  as well as a closing  certificate of
Borrower  and an  opinion  of  Borrower's  counsel  (both in form and  substance
satisfactory to Lender) with respect to this Second  Modification and the Second
Additional Term Note.

5. Additional Deliveries. Borrower shall deliver within five
(5) Business Days after the date hereof, copies of each of the original seller
notes, dated December 11, 1996, executed by 3000524 Nova Scotia Limited, a
Canadian commercial corporation and subsidiary of Borrower, in favor of Nubio
Technologies Corporation, Patrick Waddy and Blaire MacNeil, respectively, in
connection with the acquisition of all of the capital stock of Dominion
Biologicals Limited, a Canadian corporation, marked "paid in full", together
with evidence satisfactory to the Lender that all guarantees of the obligations
owed under such seller notes and all liens securing such obligations are
terminated and released.

6. Counterparts. This Second Modification may be executed in multiple
counterparts, each of which shall be deemed to be an original and all
of which when taken together shall constitute one and the same
instrument.

7. Governing Law. This Second Modification shall be governed by, and
construed in accordance with, the internal laws of the State of
Georgia applicable to contracts made and performed in such state.

<PAGE>



                  IN WITNESS WHEREOF, the parties hereto have caused this Second
Modification  to be duly executed and delivered as of the day and year specified
at the beginning hereof.

                                                  BORROWER:

                                                  IMMUCOR, INC.

                                                  By:      /s/ Edward L. Gallup

                                                  Edward L. Gallup, President


                                                  LENDER:

                                                  WACHOVIA BANK, N.A.


                                                  By:      /s/ Ernesto Moran
                                                  Title:   President



<PAGE>



                              CONSENT OF GUARANTORS


                  Each of the undersigned  guarantors does hereby consent to the
execution,   delivery  and  performance  of  the  within  and  foregoing  Second
Modification of Loan Agreement.

                  IN WITNESS  WHEREOF,  each of the  undersigned  guarantors has
executed this Consent under seal as of the day and year first above set forth.


                                        GAMMA BIOLOGICALS, INC.



                                        By: /s/  Edward L. Gallup
                                        Edward L. Gallup, President


                                        GAMMA BIOLOGICALS INTERNATIONAL, INC.


                                        By: /s/  Edward L. Gallup
                                        Edward L. Gallup, President


                                        BCA ACQUISITION CORPORATION


                                        By: /s/  Edward L. Gallup
                                        Edward L. Gallup, President