# Patent

## Title
Contrast agents based on long-lived nuclear singlet states and related methods

## Abstract

Methods are described for preparing magnetic resonance imaging (MRI) and/or magnetic resonance spectroscopy contrast agents where the contrast agents are prepared from precursor molecules having at least four non-zero-spin nuclei that form two pairs of chemically equivalent or effectively equivalent nuclei, e.g., diphenylacetylene or diethyl oxalate. The precursor molecule is hyperpolarized and a sequence of one or more radiofrequency pulses is applied to transfer spin state population between the first and second pair of nuclei, thereby providing a non-equilibrium single state nuclear spin population. To detect the contrast agent, another sequence of one or more radiofrequency pulses is applied to transfer singlet order to polarization. No transformation of the molecular structure of the contrast agent is necessary for detection. Also described are methods of imaging targets using the contrast agents.

## Background
RELATED APPLICATIONS
The presently disclosed subject matter is based on and claims the benefit of U.S. Provisional Patent Application Ser. No. 61/871,457, filed Aug. 29, 2013; the disclosure of which is incorporated herein by reference in its entirety.


GOVERNMENT INTEREST
This invention was made with government support under Grant No. CHE-1058727 awarded by the National Science Foundation and Grant Nos. T32EB001040 and R01EB02122 awarded by National Institutes of Health. The government has certain rights in the invention.


TECHNICAL FIELD
The presently disclosed subject matter relates to methods of preparing magnetic resonance imaging and/or spectroscopy contrast agents having long-lived nuclear singlet states that can be accessed by pulse sequence transformation. Also provided are the contrast agents themselves and methods of using the contrast agents.
ABBREVIATIONS


    
    
        °=degree
        13C=carbon-13
        13C-DEO=13C2-diethyl oxalate
        13C-DPA=13C2-1,2-diphenylacetylene
        CW=continuous wave
        DNP=dynamic nuclear polarization
        Hz=hertz
        M2S=magnetization-to-singlet
        MRI=magnetic resonance imaging
        ms=milliseconds
        NMR=nuclear magnetic resonance
        PET=positron emission tomography
        RF=radiofrequency
        s=seconds
        S2M=singlet-to-magnetization
        T=Tesla
        T1=longitudinal relaxation time constant
        T2=spin-spin relaxation time constant
        Ts=singlet state lifetime
    
    


BACKGROUND
Nuclear magnetic resonance (NMR), or magnetic resonance spectroscopy, is a powerful, well-established tool for studying chemical samples and sample interactions. In NMR, the spin and magnetism of atomic nuclei are exploited to provide information about the chemical composition, spatial distribution, or molecular motion of molecules or atoms. The imaging analog of NMR, magnetic resonance imaging (MRI) is a powerful technique in biomedical sample imaging.
One of the limitations of NMR and MRI is low intrinsic signal strength. Some attempts to overcome this limitation have involved the use of hyperpolarized contrast agents, which have very large nuclear polarizations and, therefore, sensitivities that are orders of magnitude higher than ordinary molecules. Polarization can persist for as long as 100 seconds in some molecules before the polarized nuclei return to thermal equilibrium.
While such a lifetime is sufficient for some imaging and/or spectroscopy studies, contrast agents with longer lifetimes are highly desirable to study additional processes of interest, for example processes related to diffusion, flow, slow molecular motion, chemical reactions, metabolism, and drug targeting and distribution, among others. The relaxation of nuclear spins back to thermal equilibrium is characterized by a time constant, T1, known as the longitudinal relaxation time constant or as the spin lattice relaxation time constant. The development of contrast agents having polarization that persists for times longer than T1 would be beneficial for both NMR and MRI.
SUMMARY
In some embodiments, the presently disclosed subject matter provides a method of providing a contrast agent for magnetic resonance imaging (MRI) or magnetic resonance spectroscopy, the method comprising: providing a molecule comprising at least four non-zero-spin nuclei X, X′, Y, and Y′, wherein X and X′ are a first pair of chemically equivalent or effectively equivalent nuclei, and Y and Y′ are a second pair of chemically equivalent or effectively equivalent nuclei; hyperpolarizing the molecule to provide a hyperpolarized molecule; and applying a first sequence of one or more radiofrequency pulses to the hyperpolarized molecule to transfer a spin state population between the first and the second pair of chemically equivalent or effectively equivalent nuclei; thereby providing a contrast agent having a non-equilibrium singlet state nuclear spin population, wherein the contrast agent molecule can be detected after the application of a second sequence of one or more radiofrequency pulses to transfer singlet order to polarization.
In some embodiments, JXX′, the scalar coupling between X and X′, is greater than JXY, the scalar coupling between X and Y; JXY′, the scalar coupling between X and Y′; JX′Y, the scalar coupling between X′ and Y; and JX′Y′, the scalar coupling between X′ and Y′. In some embodiments, JXX′ is at least three times greater than JXY, JXY′, JX′Y, or JX′Y′.
In some embodiments, the scalar coupling between X and X′ is between about 10 and about 300 Hertz (Hz). In some embodiments, X and X′ are both 13C or 15N nuclei. In some embodiments, the molecule has a molecular structure wherein X and X′ are directly bonded to each other. In some embodiments, X and X′ are free of directly bonded hydrogen atoms. In some embodiments, Y and Y′ are both 1H or 19F nuclei.
In some embodiments, the molecule has a rigid molecular structure. In some embodiments, the molecule is selected from the group consisting of a diarylacetylene, diacetylene, an oxalate diester, a cyclooctyne or a lactone or lactam analog thereof, a dibenzocyclooctyne or a lactone or lactam analog thereof, a pyridazine, a phthalazine, and a gem-difluoro compound. In some embodiments, the molecule is selected from the group consisting of diphenylacetylene and diethyl oxalate. In some embodiments, the molecule has a symmetric molecular structure, X is chemically equivalent to X′, and Y is chemically equivalent to Y′.
In some embodiments, the hyperpolarizing is performed by dynamic nuclear polarization (DNP). In some embodiments, the contrast agent can be provided and detected without undergoing a molecular structure transformation. In some embodiments, the contrast agent has a symmetrical molecular structure and can be detected without breaking the symmetry. In some embodiments, Y and Y′ are 1H nuclei, and the one or more radiofrequency pulses are hydrogen-only pulse sequences.
In some embodiments, the contrast agent has pharmaceutical activity. In some embodiments, the contrast agent is formulated in a pharmaceutically acceptable carrier and administered to a subject. In some embodiments, the subject is a mammal.
In some embodiments, the non-equilibrium singlet state nuclear spin population can persist for a time that is greater than about 3 times the longitudinal relaxation time constant T1. In some embodiments, the non-equilibrium singlet state nuclear spin population can persist for a time that is greater than about 10 times T1. In some embodiments, the non-equilibrium singlet state nuclear spin population can persist for a time that is greater than about 50 times T1. In some embodiments, the non-equilibrium singlet state nuclear spin population can persist for about 69 times T1.
In some embodiments, the presently disclosed subject matter provides a method of imaging a target, the method comprising: providing a molecule comprising at least four non-zero-spin nuclei X, X′, Y, and Y′, wherein X and X′ are a first pair of chemically equivalent or effectively equivalent nuclei, and Y and Y′ are a second pair of chemically equivalent or effectively equivalent nuclei; hyperpolarizing the molecule to provide a hyperpolarized molecule; applying a first sequence of one or more radiofrequency pulses to the hyperpolarized molecule to transfer a spin state population between the first and second pair of nuclei, thereby providing a contrast agent having a non-equilibrium singlet state nuclear spin population; contacting the contrast agent with the target; applying a second sequence of one or more radiofrequency pulses to convert singlet order to magnetization; generating a nuclear magnetic resonance signal; and detecting the nuclear magnetic resonance signal, thereby imaging the target.
In some embodiments, the target is one of a cell, a tissue, an organ, and a subject. In some embodiments, the contacting comprises administering a pharmaceutical formulation comprising the contrast agent to a subject. In some embodiments, the subject is a mammal.
In some embodiments, the hyperpolarizing is performed by dynamic nuclear polarization (DNP).
In some embodiments, JXX′, the scalar coupling between X and X′, is greater than JXY, the scalar coupling between X and Y; JXY′, the scalar coupling between X and Y′; JX′Y, the scalar coupling between X′ and Y; and JX′Y′, the scalar coupling between X′ and Y′. In some embodiments, JXX′ is at least three times greater than JXY, JXY′, JX′Y, or JX′Y′.
In some embodiments, the scalar coupling between X and X′ is between about 10 and about 300 Hertz (Hz). In some embodiments, X and X′ are both 13C or 15N nuclei. In some embodiments, the molecule has a molecular structure wherein X and X′ are directly bonded to each other. In some embodiments, X and X′ are free of directly bonded hydrogen atoms. In some embodiments, Y and Y′ are both 1H or 19F nuclei.
In some embodiments, the molecule has a rigid molecular structure. In some embodiments, the molecule is selected from the group consisting of a diarylacetylene, diacetylene, an oxalate diester, a cyclooctyne or a lactone or lactam analog thereof, a dibenzocyclooctyne or a lactone or lactam analog thereof, a pyridazine, a phthalazine, and a gem-difluoro compound. In some embodiments, the molecule is selected from the group consisting of diphenylacetylene and diethyl oxalate. In some embodiments, the molecule has a symmetric molecular structure and X is chemically equivalent to X′ and Y is chemically equivalent to Y′.
In some embodiments, the contrast agent can be detected without undergoing a molecular structure transformation. In some embodiments, the contrast agent has a symmetric molecular structure and can be detected without breaking the symmetry.
In some embodiments, Y and Y′ are 1H nuclei, the one or more radiofrequency pulses of the first and second sequences are hydrogen-only pulse sequences; and the nuclear magnetic resonance signal is a hydrogen signal.
In some embodiments, the presently disclosed subject matter provides a contrast agent prepared by the method comprising: providing a molecule comprising at least four non-zero-spin nuclei X, X′, Y, and Y′, wherein X and X′ are a first pair of chemically equivalent or effectively equivalent nuclei, and Y and Y′ are a second pair of chemically equivalent or effectively equivalent nuclei; hyperpolarizing the molecule to provide a hyperpolarized molecule; and applying a first sequence of one or more radiofrequency pulses to the hyperpolarized molecule to transfer a spin state population between the first and the second pair of chemically equivalent or effectively equivalent nuclei; thereby providing a contrast agent having a non-equilibrium singlet state nuclear spin population, wherein the contrast agent molecule can be detected after the application of a second sequence of one or more radiofrequency pulses to transfer singlet order to polarization.
Accordingly, it is an object of the presently disclosed subject matter to provide methods of providing a contrast agent for magnetic resonance imaging (MRI) or magnetic resonance spectroscopy and to provide methods of imaging a target with a contrast agent.
An object of the presently disclosed subject matter having been stated hereinabove, and which is achieved in whole or in part by the presently disclosed subject matter, other objects will become evident as the description proceeds hereinbelow.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1A is a schematic drawing of pulse sequences according to an embodiment of the presently disclosed subject matter. The sequences include a magnetization-to-singlet (M2S) sequence to convert bulk magnetization to singlet order, followed by a singlet-to-magnetization sequence (S2M) to convert singlet order to bulk magnetization. After the S2M sequence, a 90° pulse is applied and magnetization is detected (zigzagging line at right). Between the M2S and S2M sequences, there is a first z-gradient, G1, and a variable relaxation delay, τr. The variable relaxation delay can be followed by at least one additional 90° pulse and one additional z-gradient, G2. A third z-gradient (G3) and the 90° pulse immediately prior to G3 (both outlined in dotted lines) are optional. Signal acquisition is indicated by the squiggly line.
FIG. 1B is a schematic drawing showing additional details of the M2S sequence described for FIG. 1A. The M2S sequence includes a 90° pulse 90x (shown as an unshaded rectangle) followed by a first multiple echo sequence (shown in brackets), which is repeated n times. The multiple echo sequence can include a delay (τ/2, shown by the striped heavy line), a composite pulse unit (which includes a 90° pulse (unshaded rectangle), a 180° pulse (shaded rectangle), and another 90° pulse (unshaded rectangle)) and second τ/2 delay (striped heavy line). Accordingly, between each composite pulse unit in the echo sequence is a delay of τ. Following the first multiple echo sequence is a 90° pulse 90y (unshaded rectangle), another delay τ/2 (striped heavy line), and then a second multiple echo sequence (shown in the second set of brackets), which is the same as the first multiple echo sequence, only repeated half as many times (i.e., n/2).
FIG. 2 is a drawing of the molecular structure of 13C2-diphenylacetylene. The scalar coupling 1JCC (a) between the neighboring 13C atoms is 182 Hz. The scalar coupling 3JCH (b) between the 13C atom and the nearest aromatic hydrogen atom (i.e., the hydrogen three atoms away) is 5.5 Hz. The scalar coupling 4JCH (c) between the 13C and the nearest aromatic hydrogen on the more distant phenyl ring (i.e., the hydrogen four atoms away) is −0.6 Hz.
FIG. 3 is a schematic drawing of pulse sequences according to an alternative embodiment of the presently disclosed subject matter. The sequences include a 90 degree (90y) pulse followed by continuous wave irradiation (CWx) to transfer nuclear spin population into a long-lived singlet. After a variable relaxation delay (τr), a second continuous wave irradiation (CWx) is used to access the nuclear spin population. The amplitude (γB1) of the continuous wave irradiations is matched to the largest in-pair J coupling (e.g., =2πJxx).
FIG. 4 is a schematic drawing showing a flow diagram of an imaging method according to one embodiment of the presently disclosed subject matter.



DETAILED DESCRIPTION
The presently disclosed subject matter will now be described more fully hereinafter with reference to the accompanying Examples, in which representative embodiments are shown. The presently disclosed subject matter can, however, be embodied in different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the embodiments to those skilled in the art.
All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety.
Throughout the specification and claims, a given chemical formula or name shall encompass all optical and stereoisomers, as well as racemic mixtures where such isomers and mixtures exist, unless otherwise noted.
I. DEFINITIONS
While the following terms are believed to be well understood by one of ordinary skill in the art, the following definitions are set forth to facilitate explanation of the presently disclosed subject matter.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this presently described subject matter belongs.
Following long-standing patent law convention, the terms “a”, “an”, and “the” refer to “one or more” when used in this application, including the claims. Thus, for example, reference to “a pharmaceutically acceptable carrier” includes mixtures of one or more carriers, two or more carriers, and the like.
The term “comprising”, which is synonymous with “including,” “containing,” or “characterized by” is inclusive or open-ended and does not exclude additional, unrecited elements or method steps. “Comprising” is a term of art used in claim language, which means that the named elements are essential, but other elements can be added and still form a construct within the scope of the claim.
As used herein, the phrase “consisting of” excludes any element, step, or ingredient not specified in the claim. When the phrase “consists of” appears in a clause of the body of a claim, rather than immediately following the preamble, it limits only the element set forth in that clause; other elements are not excluded from the claim as a whole.
As used herein, the phrase “consisting essentially of” limits the scope of a claim to the specified materials or steps, plus those that do not materially affect the basic and novel characteristic(s) of the claimed subject matter.
With respect to the terms “comprising”, “consisting of”, and “consisting essentially of”, where one of these three terms is used herein, the presently disclosed and claimed subject matter can include the use of either of the other two terms.
The term “contrast agent” refers to a contrast agent molecule or a composition comprising a contrast agent molecule that increases the contrast of a tissue, organ, cell or other biological structure being examined, for example using nuclear magnetic resonance imaging (MRI). The contrast agent molecules of the presently disclosed subject matter can also be used to determine the progress of chemical reactions or non-biological phenomena (e.g., chemical diffusion) via nuclear magnetic resonance (NMR) spectroscopy.
The term “J-coupling” can also be referred to as spin-spin coupling or scalar coupling. As used herein, J-coupling can refer to a J coupling which is larger than the reciprocal of the spin-spin relaxation time, T2.
As used herein the term “chemically equivalent nuclei” can refer to nuclei having the same chemical shift. Accordingly, molecules having chemically equivalent nuclei generally have a plane of symmetry, such that each of the two chemically equivalent nuclei are bonded to a chemical group or groups that have the same chemical structure or structures as the group or groups bonded to the other chemically equivalent nuclei. Non-chemically equivalent nuclei are generally bonded to one chemical group that is different in structure than the chemical group or groups bonded to the other non-chemically equivalent nuclei. Non-equivalent nuclei can have a resonance frequency difference which exceeds the J coupling between them.
The term “effectively equivalent nuclei” can refer to nuclei wherein, by coincidence or choice of field strength, the resonance frequencies of the nuclei will differ by less than the J coupling between them. For example, in some embodiments, the effectively equivalent nuclei can have a chemical shift difference that is at least three times (or, in some embodiments, at least five times) smaller than the J coupling between them.
The term “effectively equivalent nuclei” can, in some embodiments, refer to nuclei that are “nearly chemically equivalent” (or “nearly equivalent”), as well as to nuclei having a chemical shift difference that can be reduced or eliminated by radiofrequency pulses. The terms “nearly chemically equivalent” and “nearly equivalent” refer to nuclei that have a chemical shift difference that is smaller than the largest J coupling in the system (i.e., in the molecule). The methods described herein can be performed using a system having nearly equivalent nuclei as one or both of the nuclei pairs in the absence of any additional irradiation.
Non-zero-spin nuclei can be selected from the group including, but not limited to, 1H, 2H, 13C, 14N, 15N, 19F, and 31P. In some embodiments, providing the molecule comprising at least four non-zero-spin nuclei comprises synthetically doping or labeling a molecule with a particular isotope (e.g., with 13C).
The terms “sensitive” and “insensitive” as used herein with regard to nuclei can refer to the relative intensity of NMR signals of the nuclei. The signal intensity can relate to the gyromagnetic ratio (γ) of the nuclei, i.e., to the ratio of magnetic dipole moment to angular momentum. Generally, nuclei (e.g., 13C, 14N, 15N, etc.) with lower gyromagnetic ratios are more insensitive, while nuclei (e.g., 1H and 19F) with higher gyromagnetic ratios are more sensitive.
In some embodiments, hyperpolarizing is performed by dynamic nuclear polarization (DNP). DNP refers to transferring spin polarization from electrons to nuclei. In some embodiments, DNP can be performed by doping a material with a free radical. The unpaired electrons in the free radical can be polarized, for example, by exposure to a high magnetic field and low temperature. Irradiation at the electron paramagnetic resonance frequency can then serve to transfer polarization to the nuclei. However, the hyperpolarizing can be performed by other techniques known in the art, such as, but not limited to, reaction with para-hydrogen (i.e., by para-hydrogen induced polarization (PHIP)). By “hyperpolarization,” it is meant that the sample is polarized to a level over that found at room temperature and 1 Tesla, for example, polarized to a polarization degree in excess of about 0.1%, in excess of about 1%, or in excess of about 10%.
The terms “molecular structure transformation” and “chemical transformation” can refer to chemical and biochemical reactions wherein one or more bonds are formed or broken.
The term “rigid structure” as used herein can refer to molecules having one or more bonds that do not have essentially free rotation. Such structures can include pi (π) bonds or bonding systems (e.g., a system of conjugated π bonds) that are typically planar and/or substructures (e.g., ring structures) that have preferred conformations, for example, due to angle strain and/or torsional strain. In some embodiments, molecules with rigid molecular structure have one or more ring structures and/or a series of alternating single and multiple bonds (e.g., double or triple bonds).
In some embodiments, the target is one of a cell, a tissue, an organ, and a subject (e.g., a human or other mammal). In some embodiments, the contrast agent can be used in an NMR study and the target can be a chemical composition (e.g., a non-biochemical reaction mixture).
II. GENERAL CONSIDERATIONS
Nuclear magnetic resonance (NMR) and the imaging analog, magnetic resonance imaging (MRI), have become important techniques. MRI has become a powerful clinical imaging modality for two fundamental reasons. First, the hardware is mature: modern MRI machines routinely give complex sequences of arbitrarily shaped radiofrequency pulses to create precise excitation, and give magnetic field gradient pulses to suppress magnetization or obtain spatial resolution. More importantly, however, the theoretical framework is mature. No other modern spectroscopy has such a strong theoretical basis, which of course is used to understand the structures of molecules as complicated as proteins in solution. This maturity is even more important in MRI: complications associated with imaging in vivo can often be reduced or eliminated by clever pulse sequence design.
However, the maturity of that theoretical framework also implies that the known limitations of MRI are rather fundamental. The Boltzmann distribution implies that the net fractional magnetization is small at room temperature, so in most MRI studies, the signal arises mostly from water. Contrast then arises primarily from parameters that can be traced back to the spin physics explorations of the 1940s and 1950s (the local bulk magnetization Mo, the relaxation parameters T1, T2 and T2*, and local values of diffusion, sometimes in different directions), which often only have very indirect clinical relevance or correlation with metabolism and cell biochemistry. MRI contrast agents generally have limited specificity, and usually need to be present in high concentration to affect the signal.
These limitations have recently been partially surmounted by the ready commercial availability of hyperpolarized reagents, which have very large nuclear polarizations and thus orders of magnitude higher sensitivity than ordinary molecules. Among the recent methods are techniques to create spin polarized 3He (see McFall et al., Radiology, 200, 553-558 (1996); and Salerno et al., Eur. J. Radiol., 40, 33-44 (2001)), para-H2 addition across double bonds (see Bowers and Weitekamp, Phys. Rev. Lett., 57, 2645-2648 (1986); Bowers and Weitekamp, J. Am. Chem. Soc., 109, 5541-5542 (1987); Natterer and Bargon, Progr. Nucl., Magn. Res. Spectrosc., 31, 293-315 (1997); Duckett and Sleigh, Progr. Magn. Res. Spectrosc., 34, 71-92 (1999); and Golman et al., Magnetic Resonance in Medicine, 46, 1-5 (2001)), and dynamic nuclear polarization (DNP). See Abragam and Goldman, Rep. Prog., Phys., 41, 395-467 (1978); de Boer et al., Journal of Low Temperature Physics, 15, 249-267 (1974); de Boer and Niinikoski, Nuclear Instruments and Methods, 114, 495-498 (1974); Hall et al., Science, 276, 930-931 (1997); Bajaj et al., Journal of Magnetic Resonance, 160, 85-90 (2003); Johansson et al., Magn. Reson. Med., 51, 464-472 (2004); and Ardenkjaer-Larsen et al., Proc. Natl. Acad. Sci., USA, 100, 10158-10163 (2003). All of these methods have demonstrated large nuclear magnetization (>10%, as compared to typical thermal magnetization of 10−5, with the polarization persisting as long as 100 s in some molecules. Many different research groups have been examining potential uses of such hyperpolarized molecules both in vivo and in vitro. See Kurhanewicz et al., J. Nucl. Med. 49(3), 341-344 (2008); Golman et al., Cancer Research, 66, 10855-10860 (2006); Merritt et al., Proc. Natl. Acad. Sci., USA, 104, 19773-19777 (2007); Day et al., Mag. Res. Chem., 45(12), 1018-1021 (2007); Gabellieri et al., J. Am. Chem. Soc., 130(14), 4598 (2008).
DNP methodology in particular is very versatile, and hundreds of different molecules have been polarized. However, most DNP studies have focused on 13C in pyruvate, largely because the T1 relaxation time for the C1 position is relatively long (40 s at 14.1 T), so the polarized nuclei can potentially undergo many reactions before the NMR signal returns to thermal equilibrium and becomes undetectable. Generically, carbon-13 T1 values are expected to be tens of seconds for carbons without attached protons, and much shorter with attached protons. While this lifetime permits some important metabolic processes to be studied, it is vastly shorter than the lifetimes associated with other molecular imaging modalities (e.g., 18F PET) and provides a fundamental limitation to the ultimate generality of the technique.
Previous studies have demonstrated the use of singlet states comprised of non-symmetry related spins to lengthen T1. See Ahuja, et al., J. Chemical Physics, 127, 134112 (2007); Carravetta et al., Physical Review Letters, 92, 153003 (2004); Carravetta and Levitt, J. Am. Chem. Soc., 126, 6228-6229 (2004); and Carravetta and Levitt, J. Chem. Physics, 122, 214505 (2005). In the studies, molecules with broken symmetry (for example, a single carbon-13) are used. The non-symmetry related spins are manipulated to appear to be equivalent by removing frequency differences with multiple spin echoes or by lowering the magnetic field so much that the resonance frequencies are essentially the same (which requires removing the sample from the magnet). The signal is then observed by permitting free evolution in a high field. Both approaches give interesting demonstrations of lifetime increases, but neither is practical for MRI. In addition, at the microscopic level, both of these approaches have certain limitations. For example, relaxation is dominated by the local components of the magnetic field fluctuating near the Larmor frequency, and, if two sites are physically inequivalent, these fluctuations are expected to be poorly correlated, even if the resonance frequencies are nearly the same.
PCT International Patent Application Publication No. WO 2005/015253 relates to an approach that involves lowering the field to create a pseudo-singlet state and to reacting an unsaturated symmetric molecule with parahydrogen to provide a quasi-equilibrium nuclear spin ensemble estate. See also, Carravetta et al. Physical Review Letters, 92, 153003 (2004); Carravetta and Levitt, J. Am. Chem. Soc., 126, 6228-6229 (2004); and Carravetta and Levitt, J. Chem. Physics, 122, 214505 (2005).
PCT International Patent Application Publication No. WO 2010/014893 describes a method of imaging a target wherein a contrast agent is provided by preparing a compound having a non-equilibrium singlet state nuclear spin population wherein the singlet is between chemically equivalent spins. To detect the contrast agent, a chemical reaction is performed to break symmetry in the contrast agent. Thus, for example, the contrast agent can be provided by chemically converting a hyperpolarized molecule having two J-coupled, non-zero-spin, non-equivalent nuclei into a molecule wherein the previously non-equivalent nuclei are equivalent (e.g., by converting a non-symmetric molecule into a symmetric molecule). Then, magnetization stored in the contrast agent can be accessed by chemically converting the contrast agent into a detection molecule where the equivalent nuclei become non-equivalent again.
II.A. Accessing Singlet States Between Chemically Equivalent Spins without Breaking Symmetry
According to the presently disclosed subject matter it is possible to transfer population in and out of chemically equivalent singlet states using only radiofrequency pulses. Thus, no molecular structure transformation of the contrast agent is required for detection (or formation). Accordingly, the presently disclosed methods can be performed without making, breaking or isomerizing any bonds in the contrast agent.
More particularly, the contrast agent of the presently disclosed subject matter can comprise at least two pairs of chemically equivalent or effectively equivalent non-zero-spin nuclei. According the presently disclosed methods, it is possible to create a coherence involving one pair of nuclei (e.g., carbon-13, nitrogen-14, nitrogen-15, deuterium (i.e., 2H), or some other relatively insensitive nuclei) and a second pair of nuclei (e.g., hydrogen or fluorine-19 or another more sensitive nuclei) that can be accessed completely through the sensitive nuclei, detected completely through the sensitive nuclei, or both, but has the longer lifetime associated with a singlet of the more insensitive nuclei.
More particularly, the presently disclosed subject matter is based on the ability to transfer population in and out of a singlet state at high field using only radiofrequency pulse sequences in molecular systems that comprise appropriate scalar couplings from the singlet spins to other spins in the molecule to break magnetic equivalence. An exemplary four-spin molecular system is 13C-diacetylene, i.e., H—C≡13C—13C≡C—H, where the 13C and H pairs are symmetric around the same center of inversion. The “singlet-singlet” state for this system, i.e., |SS=|(αβ−βα)C(αβ−βα)H/2, has no dipole-allowed transitions to other states, but does have the same overall inversion symmetry as the triplet-triplet state (i.e., |T0T0=|(αβ+βα)C(αβ+βα)H/2, which has dipole-allowed transitions. Thus, unlike the singlet state in a two-spin system, the singlet state in such a four-spin system is not isolated by symmetry from all other states. Accordingly, given appropriate scalar couplings, the “singlet-singlet” state of four-spin systems can essentially be an eigenstate. However, because this state has the same overall symmetry as the allowed triplet-triplet state, many different pulse sequences are capable of transferring population between them, such as the exemplary sequences described below. Additional theoretical considerations regarding four-spin molecular systems are described in Feng et al. (Nature Physics, 2012, 8, 831).
In some embodiments, the pulse sequences that can be used to migrate population between singlet and triplet states are similar to magnetization-to-w singlet-singlet-to-magnetization sequences (M2S-S2M) used to interconvert between slightly chemically equivalent spins (see Tayler and Levitt (Phys. Chem. Chem. Phys. 2011, 13, 5556); and Pileio et al. (Proc. Natl. Acad. Sci. USA, 2011, 107, 17135)), except that, according to the presently disclosed subject matter, chemical equivalence is preserved and magnetic equivalence is broken using scalar couplings. Thus, a train of 180° composite pulses separated by delays to allow accumulation of state mixing can interconvert the singlet and triplet states. As described in Example 1, below, interconversion between 13C magnetization and 13C2-singlet order can be performed in the exemplary CC′H2H2′ spin system in 13C2-diethyl oxalate (13C2-DEO). Further, in some embodiments, the sequences can be selectively pulsed on either the insensitive nuclei (e.g., 13C) or the sensitive nuclei (e.g., 1H). Thus, as shown in Example 2, using the exemplary system of 13C2-1,2-diphenylacetylene (13C-DPA), 1H magnetization can be transferred into 13C2 singlet polarization. Therefore, according to the presently disclosed subject matter, either 13C or 1H magnetization can be hyperpolarized and converted to 13C singlet polarization, which, for detection, can subsequently be converted back into either 13C or 1H magnetization.
FIG. 1A shows a M2S-S2M sequence that can be used according to the presently disclosed subject matter. The M2S sequence is separated from the S2M sequence by a z-gradient G1, a relaxation delay τr, and a combination of one or more additional z-gradients (e.g., G2 and optional G3 in FIG. 1A) and 90° pulses. The z-gradients and 90° pulses can eliminate residual triplet magnetization as well as any thermal magnetization that grows during the relaxation delay. In particular, the first z-gradient, G1, can suppress single-quantum coherence generated by M2S. The 90° pulses have little effect on the polarization stored in the singlet state after the M2S sequence. After the S2M sequence (which can be a time-reversed M2S sequence), to convert single state population back to magnetization, a final 90° pulse provides for detection of the bulk magnetization.
The M2S sequence is shown in more detail in FIG. 1B. The M2S sequence includes a 90° pulse (i.e., 90x) followed by a first multiple echo sequence (first set of brackets). The multiple echo sequence can be equivalent to a Carr-Purcell-Meiboom-Gill (CPMG) sequence with each echo pulse composed of a [90018090900]φ composite pulse unit, where φ is phase-cycled according to a composite pulse decoupling (CPD) scheme (i.e., MLEV4). The first multiple echo sequence is followed by a 90° pulse (90y), which has a 90° phase shift compared to the first 90° pulse, 90x. A second multiple echo sequence (second set of brackets) follows an interpulse delay (τ/2). The second multiple echo sequence has half as many pulses as the first multiple echo sequence.
The inter pulse delay and n are calculated based on the coupling constants of the particular molecule being used. For instance, in molecules wherein the more sensitive nuclei is 1H and the less sensitive nuclei is 13C, τ can be calculated using the following formula:

τ=1/(2√{square root over ((J2CC+(JCH−J′CH)2)}).

In the exemplary molecule 13C2-DPA, JCC is 182 HZ, JCH=5.5 Hz, and J′CH is −0.6 Hz (see FIG. 2) providing τ=1.365 ms. In the formula above, J2CC can be replaced by JCC±(JHH), e.g., if JHH≠0. The number of pulses n can be calculated using the formula:

n=π/(2×arctan((JCH−J′CH)/JCC)).

For 13C-DPA, n=47.9. For practical purposes, this can be rounded up to 48. Similarly to the formula for τ, if JHH≠0, the term JCC can be replaced by JCC±(JHH).

In some embodiments, the relaxation delay, τr, can be calculated using the formula:

τr=1/(2√{square root over ((JCC±JHH)2+(JCH−J′CH)2)}.

In some embodiments, the presently disclosed subject matter provides a method of providing a contrast agent for magnetic resonance imaging (MRI) or magnetic resonance spectroscopy, the method comprising: providing a molecule comprising at least four non-zero-spin nuclei X, X′, Y, and Y′, wherein X and X′ are a first pair of chemically equivalent or effectively equivalent nuclei, and Y and Y′ are a second pair of chemically equivalent or effectively equivalent nuclei; hyperpolarizing the molecule to provide a hyperpolarized molecule; and applying a first sequence of one or more radiofrequency pulses to the hyperpolarized molecule to transfer a spin state population between the first and the second pair of chemically equivalent or effectively equivalent nuclei; thereby providing a contrast agent having a non-equilibrium singlet state nuclear spin population, wherein the contrast agent molecule can be detected after the application of a second sequence of one or more radiofrequency pulses to transfer singlet order to polarization.
The first sequence of one or more pulses is designed to put the molecule into a long-lived state. In a typical MRI application, the molecule can be injected into a subject at that point, and allowed to undergo various biochemical transformations, in some embodiments producing “metabolites.” Thus, in useful applications, there can generally be a delay (e.g., a variable relaxation delay) between the first and second sequence of pulses. This delay can be many seconds or preferably many minutes. In order to see the signal, the second sequence of one or more radiofrequency pulses is applied to transfer singlet order back into an observable state.
In some embodiments, a metabolic reaction can change the molecular structure of the contrast agent to make chemically equivalent nuclei inequivalent, in which case, the long-lived state can be lost. Alternatively, in some embodiments, the metabolic reaction can change the molecular structure of the contrast agent, thereby changing resonance frequencies of some of the spins, in which case the metabolite can still have a long-lived state, but can be distinguishable from the original contrast agent molecule during detection. Accordingly, in some embodiments, “detection” of the contrast agent molecule refers to detection of a MRI or NMR signal of a molecule having the same molecular structure as the original molecule (i.e., the molecule with the four non-zero-spin nuclei). However, the contrast agent molecule can also be “detected” via detection of a MRI or NMR signal from a molecule that is a derivative of the original molecule, e.g., a metabolite formed when the contrast agent has undergone a biochemical transformation; or via the absence of an expected signal, e.g., when formation of a metabolite of the contrast agent molecule results in early loss of a long-lived singlet state.
In some embodiments, a pulsed field gradient (e.g., a z gradient) is applied to the contrast agent after the first sequence of one or more pulses is applied, but prior to the delay between the first and second sequences. In some embodiments, a pulsed field gradient (e.g., a z gradient) and a 90° pulse are applied to the contrast agent after the delay between the first and second sequence. In some embodiments, the post-delay pulsed field gradient and 90° pulse are repeated one or more times.
In some embodiments, the “effectively equivalent nuclei” can be “nearly equivalent” (e.g., having a chemical shift difference that is smaller than the largest J coupling in the molecule) or can have a chemical shift difference that can be reduced by radiofrequency pulses. In some embodiments, such as when the hyperpolarization provides large longitudinal order (e.g., when the hyperpolarization is by DNP), the first and second pulse sequences (e.g., M2S and S2M in FIG. 1a) can be the same sequence, simply reversed in time. Thus, in some embodiments, the same pulse sequence is used to convert conventional magnetization to the singlet and to convert from the singlet to conventional magnetization. In some embodiments, such as when hyperpolarization is by reaction with para-hydrogen, the first and second sequences can be different. For example, the M2S sequence can be shortened (or even completely omitted) compared to the S2M sequence when hyperpolarization is performed with para-hydrogen. However, in some embodiments, the first sequence includes at least one or more or at least two or more radiofrequency pulses.
In some embodiments, e.g., to reduce energy dissipation, the M2S and S2M sequences can be replaced or modified using spin-lock induced crossing (SLIC) sequences that employ continuous wave (CW) irradiation. See Theis et al. (J. Chem. Phys. 2014, 140, 014201). Thus, in some embodiments (see FIG. 3), a 90° pulse (to create transverse magnetization) is followed by radiofrequency (RF) irradiation at a smaller amplitude to transfer nuclear spin population into a long-lived singlet. The RF irradiation (i.e., CWx in FIG. 3) can be 90° out of phase from the first pulse (i.e., along the direction of magnetization). The CW irradiation can be performed with a locking field based on JXX. Following a variable relaxation delay, an identical RF irradiation can be used to allow for detection of MRI or NMR signal.
Typically, X and X′ are less sensitive than Y and Y′. In some embodiments, JXX′, the scalar coupling between X and X′ the less sensitive pair of chemically equivalent or effectively equivalent nuclei, is greater than JXY, the scalar coupling between X and Y; JXY′, the scalar coupling between X and Y′; JX′Y, the scalar coupling between X′ and Y; and JX′Y′, the scalar coupling between X′ and Y′. In some embodiments, JXX′ is at least three times greater than JXY, JXY′, JX′Y, or JX′Y′. In some embodiments, Jxx′ can be less than three times greater than JXY, JXY′, JX′Y, or JX′Y′, such as when JXY, JXY′, JX′Y, or JX′Y′ can be reduced by applying radiofrequency pulses (e.g., only to Y and Y′). In some embodiments, the scalar coupling between X and X′ is between about 10 and about 300 Hertz (Hz) (e.g., about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, or 300 Hz or a range involving any combination thereof).
In some embodiments, X and X′ are both 13C or 15N nuclei. In some embodiments, X and X′ are directly bonded to each other (e.g., via a nitrogen-nitrogen single bond, a carbon-carbon single bond, or by a carbon-carbon triple bond). In some embodiments, X and X′ are free of directly bonded hydrogen atoms. In some embodiments, X and X′ are nuclei with a spin >1⁄2 (e.g., 14N or 2H). In some embodiments, Y and Y′ are both 1H or 19F nuclei.
In some embodiments, there can be more than one pair of the more sensitive nuclei. For example, the contrast agent can be diphenylacetylene, wherein there are two carbons coupled to four chemically equivalent hydrogens and a long-lived carbon singlet can be populated from the hydrogens.
In some embodiments, the molecule has a rigid molecular structure. For example, the molecule can have a structure comprising one or more rings (i.e., one or more aromatic or non-aromatic ring structures) and/or one or more pi bonds (such as in an alkene or alkyne group). In some embodiments, the molecule is selected from the group comprising diarylacetylene, diacetylene, an oxalate diester, a cyclooctyne or a lactone or lactam analog thereof, a dibenzocyclooctyne or a lactone or lactam analog thereof, a pyridazine, a phthalazine, and a gem-difluoro compound. By “lactone or lactam analog thereof” is mean that two carbon atoms in the cyclooctyne ring are replaced by —C(═O)—O— or —C(═O)—NH—. In some embodiments, the molecule has a symmetric molecular structure. In some embodiments, X is chemically equivalent to X′ and Y is chemically equivalent to Y′. Thus, in some embodiments, the contrast agent has a symmetrical molecular structure and can be detected without breaking the symmetry. In some embodiments, the molecule is selected from the group comprising diphenylacetylene and diethyl oxalate (e.g., 13C labelled diphenylacetylene or diethyl oxalate).
Molecular structures of exemplary molecules that can be used as contrast agents according to the presently disclosed subject matter are shown in Table 1, below. The 13C and 15N atoms are indicated by * markings. Also included in Table 1 are estimated Ts/T1 ratios, which are calculated at magnetic fields (B0) of 8.45 T or 3 T (unless otherwise indicated) and ratios of scalar couplings.







TABLE 1







Exemplary Molecules with Isolated 13C or 15N Spin Pairs and Suitable


Scalar Couplings.













JCC(NN)/



Ts/T1 (B0 =
Ts/T1
JCH(NH)


Structure
8.45 T)
(B0 = 3T)
JNN/Δω

















4.8
14.5
3.8


 






3.6
8.9
28


 






7.2
9.1
6


 






—
12**
4.3**


 






—
16†
5.4, 29†


 






22.9

33.1


 






6.4 

29.9









Time constants in are derived from the inverse of matrix elements of the incoherent relaxation superoperator. Relaxation due to coherent oscillation is disregarded because it is quenched by dominant scalar couplings across the singlet spin pair. **The two 15N spins have an estimated chemical shift difference Δω of 3 ppm. The Ts/T1 ratio is estimated at a field strength of 0.5 T, leading to a JNN/Δω ratio around 4.3. †A chemical shift difference of about 2 ppm is estimated between the 13C2 spin pair. Estimations of lifetime extension are made at field strength of 1.5 T, where JCC/JCH≅29 and JCC/Δω≅5.4.

In some embodiments, Y and Y′ are 1H nuclei, and the one or more radiofrequency pulses (e.g., of the first and/or second sequences) are hydrogen-only pulse sequences. Thus, in some embodiments, 1H magnetization can be converted into 13C2-singlet or 15N2-singlet polarization and then converted back into 1H magnetization for detection. Accordingly, in some embodiments, the presently disclosed contrast agents and methods can be used with NMR and MRI equipment that does not possess heteronuclear capabilities, e.g., with standard clinical MRI imagers). The signals of the aromatic protons of many of the presently disclosed contrast agents can be readily distinguished from noise due to water and/or fat.
In some embodiments, the contrast agent has pharmaceutical activity. For instance, the molecule having the at least four non-zero-spin nuclei X, X′, Y, and Y′ can be a modified version of a molecule with a known pharmaceutical activity. In some embodiments, the molecule with known pharmaceutical activity can be modified by being labeled or doped with a suitable isotope (e.g., 13C or 15N). In some embodiments, the molecule with known pharmaceutical activity can be modified by being conjugated (covalently or non-covalently) to a system having at least four non-zero-spin nuclei. For instance, derivatives of 13C2-DPA can be prepared, e.g., with aromatic substituents having functional groups that can be used to conjugate the 13C2-DPA to another molecule that has biological activity, without significantly disturbing the spin system of the 13C2-DPA. Examples of pharmaceutically active molecules that can be modified to include a suitable system having at least four non-zero-spin nuclei include, but are not limited to, various antibiotics, e.g., the UDP-(3-O—(R-3-hydroxymyristoyl))-N-acetylglucosamine deacetylase (LpxC) inhibitor CHIR-090, and anticancer drugs, e.g., suberolyanilide hydroxamic acid (SAHA), also known as Vorinostat. The structures of CHIR-090 and SAHA are shown below in Scheme 1.




In some embodiments, the contrast agent is formulated in a pharmaceutically acceptable carrier and administered to a subject, such as a human or other mammal. In some embodiments, the term “pharmaceutically acceptable carrier” refers to a carrier that is pharmaceutically acceptable in humans, including water, saline and aqueous solutions that can comprise other diluents in addition to water, including, but not limited to, ethanol, propylene glycol, glycerin, and the like.
In some embodiments, the contrast agent molecule can be encapsulated into a delivery format or vehicle to protect the contrast agent molecule from one or more conditions in an in vivo environment or to aid in delivery of the contrast agent molecule to a specific location in a subject (e.g., to a particular organ or to tumor). Thus, the delivery format can include one or more targeting groups (e.g., an antibody, antigen, receptor ligand, enzyme substrate, or the like).
In some embodiments, the non-equilibrium singlet state nuclear spin population can persist for a time that is greater than about 3 times T1, greater than about 5 times T1, greater than about 10 times T1, greater than about 20 times T1, greater than about 30 times T1, greater than about 40 times T1, or greater than about 50 times T1. In some embodiments, the non-equilibrium singlet state nuclear spin population can persist for about 69 times T1.
II.B. Imaging Methods
The presently disclosed subject matter provides, in some embodiments, a method of imaging a target. In some embodiments, the method comprises: providing a molecule comprising at least four non-zero-spin nuclei X, X′, Y, and Y′, wherein X and X′ are a first pair of chemically equivalent or effectively equivalent nuclei, and Y and Y′ are a second pair of chemically equivalent or effectively equivalent nuclei; hyperpolarizing the molecule to provide a hyperpolarized molecule; applying a first sequence of one or more radiofrequency pulses to the hyperpolarized molecule to transfer a spin state population between the first and second pair of nuclei, thereby providing a contrast agent having a non-equilibrium singlet state nuclear spin population; contacting the contrast agent with the target; applying a second sequence of one or more radiofrequency pulses (e.g., to the contrast agent or to the target) to convert singlet order to magnetization; generating a nuclear magnetic resonance signal; and detecting a nuclear magnetic resonance signal, thereby imaging the target.
The target can be selected from the group comprising a cell, a tissue, an organ, and a subject (e.g., a human or other mammal). Thus, in some embodiments, the contacting comprises administering a pharmaceutical formulation comprising the contrast agent to a subject or administering an encapsulated and/or targeted contrast agent to a subject. Thus, in some embodiments, such as when the contrast agent is for use in an in vivo MRI study, the method can further comprise incorporating the contrast agent molecule into a pharmaceutically acceptable carrier or delivery vehicle to provide a pharmaceutically acceptable formulation suitable for administration to a subject.
In some embodiments, the subject is a mammal. In some embodiments, the mammal is a human; however, the presently disclosed subject matter also relates to the administration of contrast agents to any mammal (e.g., dogs, cats, horses, cows, goats, sheep, and the like, including both animals kept on farms and in zoos). Thus, the presently disclosed MRI contrast agents can be used in both medical and veterinary practice.
The formulation can include stabilizers, antioxidants, osmolality adjusting agents, solubilizing agents, emulsifiers, viscosity enhancers, buffers, etc. The formulation can be in forms suitable for parenteral (e.g., intravenous or intraarterial) or enteral (e.g., oral or rectal) application, for example for application directly into body cavities or for injection or infusion into the cardiovascular system. The formulation can be free of paramagnetic, superparamagnetic, ferromagnetic, and/or ferromagnetic contaminants. In some embodiments, the administration is parenteral, e.g., via bolus injection, by intravenous or intra-arterial injection, or, if the lungs are to be imaged, by spray, e.g., via aerosol spray.
For use in in vivo imaging, the formulation can be isotonic or slightly hypertonic and/or can be administered at a concentration sufficient to yield a 1 micromolar to 1 M concentration of the MR contrast agent in the imaging zone. Precise concentration and dosage can vary depending upon, for example, toxicity, the organ targeting ability of the contrast agent or its delivery vehicle, the tissue or organ being imaged, and the administration route. In general, any suitable concentration that yields a detectable signal can be used. In some embodiments, the concentration can be between about 0.1 mM and about 10 M, e.g., between about 0.2 mM and 1 M or between about 0.5 mM and about 500 mM. Formulations for intravenous or intraarterial administration can be between about 10 mM to 10 M, e.g., between about 50 mM and about 500 mM. For bolus injections, the concentration can be between about 0.1 mM and about 10 M, e.g., between about 0.2 mM and about 10 M, between about 0.5 mM and about 1 M, between about 1.0 mM and about 500 mM, or between about 10 mM and about 300 mM. Suitable aqueous carriers can include, but are not limited to, sodium chloride solution, Ringer's solution, dextrose solution, dextrose and sodium chloride solution, lactated Ringer's solution, etc.
The contrast agent/molecule with four non-zero-spin nuclei can have a structure as described hereinabove in Section II.A. In some embodiments, the contrast agent has pharmaceutical activity or is conjugated to a molecule with pharmaceutical activity. Thus, in some embodiments, the imaging can provide information regarding drug delivery. In some embodiments, the contrast agent can target particular biomolecules. For example, the contrast agent can be a cyclooctyne derivative, such as one of the cyclooctyne derivatives in Table 1, above. Such molecules can undergo a strain-promoted azide-alkyne reaction and can be used for labeling of particular biomolecules. Thus, in some embodiments, a cyclooctyne derivative can be used according to the presently disclosed methods as an in vivo molecular imaging agent. In some embodiments, the signal from the contrast agent can vary (e.g., the chemical shift can vary) in response to physiological conditions (e.g., pH, temperature, metabolism, calcium concentration, oxygen tension, etc.). Information about the physiological conditions detected with the contrast agent can provide information about disease state. For example, pH can be used as a general disease marker, while metabolic information detected using the contrast agents can be used to detect cancer.
In some embodiments, the contrast agent can be used in an NMR study and the target can be a chemical composition (e.g., a non-biochemical reaction mixture or an environmental sample, such as a water sample from a lake, stream, river, ocean, residential water supply, or industrial site). Therefore, in some embodiments, the contacting comprises mixing a contrast agent into an ex vivo sample, such as a reaction aliquot or environmental sample. In some embodiments, the contrast agent can be dissolved in a suitable solvent prior to the mixing.
FIG. 4 shows a schematic diagram of an imaging method 400 of the presently disclosed subject matter. As shown in FIG. 4, in step 410, a suitable molecule can be provided, e.g., a molecule with at least four non-zero-spin nuclei, wherein the at least four non-zero-spin nuclei include a first pair of chemically equivalent or effectively equivalent nuclei and a second pair of chemically equivalent or effectively equivalent nuclei. The providing can include labelling, doping and/or conjugating the molecule to another molecule (e.g., a molecule with pharmaceutical activity). The molecule can have a structure as described above in Section II.A (e.g., have suitable scalar couplings and/or a rigid structure). In step 420, the molecule is hyperpolarized, e.g., via DNP.
So that the molecule remains sufficiently polarized, the molecule can be contacted with (e.g., administered to) the target as soon as possible. Thus, in some embodiments, step 420 is performed in close proximity to NMR or MRI equipment. Alternatively, the hyperpolarized molecule can be stored and/or transported to the target at low temperature (e.g., in liquid nitrogen). Prior to administration to a subject, the hyperpolarized molecule can be warmed to physiological temperatures, e.g., using infrared or microwave radiation. After contact with the target, the remaining steps, i.e., step 430, application of a first sequence of one or more radiofrequency pulses (e.g., to convert magnetization of singlet order); step 440, application of a second sequence of one or more radiofrequency pulses (e.g., to convert singlet order to magnetization), step 450, generation of a NMR or MRI signal; and step 460, detection of the signal, can be performed.
In other embodiments, magnetization can be converted to singlet order at the site of hyperpolarization (e.g., within the hyperpolarizer) to avoid loss of magnetization. Therefore, in some embodiments, the molecule can be contacted to a target after step 430, but before steps 440, 450, and 460.
In some embodiments, the first and/or second sequences are hydrogen-only pulse sequences and the signal being generated or detected is a hydrogen signal. Thus, in some embodiments, the method can be performed in a conventional clinical MR imager without heteronuclear capability.
In some embodiments, the presently disclosed subject matter provides a contrast agent, prepared according to the methods disclosed herein, wherein the contrast agent can be used to enhance a signal in one of magnetic resonance imaging or magnetic resonance spectroscopy.
EXAMPLES
The following Examples have been included to provide guidance to one of ordinary skill in the art for practicing representative embodiments of the presently disclosed subject matter. In light of the present disclosure and the general level of skill in the art, those of skill can appreciate that the following Examples are intended to be exemplary only and that numerous changes, modifications, and alterations can be employed without departing from the scope of the presently disclosed subject matter.
Example 1
13C2-Diethyl Oxylate
Studies with 13C2-diethyl oxylate (DEO) were performed. Briefly, for thermal polarization spectra, 13C2-DEO (Isotec Inc., Miamisburg, Ohio, United States of America) was dissolved in undegassed dimethyl sulfoxide (DMSO)-d6 at a 2 M concentration and spectra were acquired on a Bruker 360 MHz ( ̃8.45 T) magnet (Bruker Biosciences Corporation, Billerica, Mass., United States of America) with a 5 mm 1H and X broadband probe. Hyperpolarized spectra were acquired on a 7 T Bruker Biospect small animal MRI scanner (Bruker Biosciences Corporation, Billerica, Mass., United States of America) using samples containing 4 μl 13C2-DEO (Isotec Inc., Miamisburg, Ohio, United States of America) 1 μl DMSO (Sigma-Aldrich, St. Louis, Mo., United States of America) and 15 mM Finland Radical (GE Healthcare, Little Chalfont, United Kingdom). Hyperpolarization was performed using a Hypersense hyperpolarizer (Oxford Instruments Molecular Biotools, Enysham, United Kingdom), after which the sample was dissolved in 3 ml additional DMSO at 140° C. to provide a solution of 0.8 mM hyperpolarized DEO. The liquid state polarization of the hyperpolarized DEO, 40 s after dissolution was measured to be 3,300 times greater than thermal polarization.
To determine the relaxation rate of the singlet state, a line was least-squares fitted to a plot of ln(Shyperpolarized, singlet/SThermal) as a function of τr. Shyperpolarized, singlet/SThermal was determined from the equation: Shyperpolarized, singlet/SThermal=3,300×(sin(5°)/sin(90°))×(S(MSM)/S(5°-acquire)) where S(MSM) is the signal after the M2S-S2M sequence and S(5°-acquire) is the signal of a 5°-acquire pulse sequence performed 100 ms before the M2S-S2M sequence.
Simulated spectra 13C2-DEO were provided by initial prediction of chemical structure and nuclear parameters (e.g., chemical shielding, scalar couplings, etc.) by density functional theory (DFT) methods using Gaussian 09 (Gaussian, Inc., Wallingford, Conn., United States of America). The SPINACH software library for simulation of spin dynamics (see Hogben et al., J. Magn. Reson., 2011, 208, 179) was then used to simulate the effect of the M2S-S2M sequence and predict the relaxation profile of the compound based on the predicted parameters assuming a 40 picosecond rotational correlation time.
For 13C2-DEO, JCC=101.6 Hz and JCH=3.4 Hz (e.g., between each carbon and the adjacent methylene proton). Interconversion between the triplet and singlet of 13C2-DEO was manifested in the 13C NMR spectra by an inversion of the two middle side peaks of the pentet signal. An interpulse delay τ of 4.92 ms induced the most efficient exchange between the carbon singlet and triplet manifolds in both the experimental and simulated spectra. Also, in both experimental and simulated spectra, the maximum conversion appeared after 45 echo pulses of the initial part of the M2S sequence (i.e., n=45, n/2=22). Signal decay after the complete M2S-S2M sequence showed slow singlet order decay after an initial fast triplet signal decay both experimentally and via simulation. Based on the experimental data, Ts is around 50.6 s and T1 around 22.2 s. Based on the simulation data, Ts is about 110.2 s and T1 about 17 s. Without being bound to any one theory, these differences are believed to be due to the dipole-dipole interactions with remote methyl protons that were disregarded in the simulation and the lack of degassing of the solvent.
Example 2
13C2-DPA
1,3-Diphenyl-13C2-ethyne (13C2-DPA) was prepared. Briefly, 13C2-ethynylbenzene was combined with iodobenzene (1.05 eq) in the presence of PdCl2(PPh3)2 (0.02 eq.) and piperidine (3 eq.) under an inert atmosphere and heated to 70° C. The reaction mixture was stirred at 70° C. for 10 minutes, and then the resulting semi-solid was diluted with 20 ml of water and extracted with ethyl acetate. The organic layers were washed with 1 N HCL, water, and brine and dried over anhydrous sodium sulfate. After evaporation of the solvent, the crude product was purified by chromatography (elution with hexanes) to provide 80 mg (89% yield) of white solid 1,3-diphenyl-13C2-ethyne.
For NMR studies, the M2S-S2M sequence of FIG. 1A, including three z-gradients G1, G2, and G3, was used. More particularly, for 13C2-DPA studies, G1=3 ms, G2=4.5 ms, and G3=11 ms. The z-gradients have odd relationships to avoid gradient refocused echoes. Using the formulae described hereinabove for n and τ and the above-noted J couplings for 13C2-DPA, n was calculated as 47.9 and as 1.365 ms. Experimentally, these values were adjusted to n=48 and τ=1.38 ms (which includes 40 μs for the composite pulses). For some studies, proton decoupling was performed with continuous wave irradiation using a decoupling power of νRF=0.5 Hz.
The normal one-dimensional 13C NMR spectra of 13C2-DPA provided the expected pentet centered at 89.4 ppm. After the sequence of FIG. 1A is applied (with a 20 s delay τr between M2S and S2M) where all of the pulses are applied at the 13C Larmor frequency, the spectra shows a doublet, as only the magnetization detected as the doublet is perturbed by the sequence and stored as singlet polarization. When the sequence of FIG. 1A is performed with the pulses in the M2S applied at the 1H Larmor frequency and the remaining pulses in the 13C Larmor frequency, the spectra is also a doublet; however, the signal intensity is about 4 times larger than when all of the pulses are applied at the 13C Larmor frequency. Without being bound to any one theory, this is believed to be due to the higher initial 1H polarization compared to the initial 13C polarization. That the signal is also a doublet confirms that the source of the signal is 13C2 singlet order.
Using simulations performed in a manner similar to those for 13C2-DEO, Ts was predicted to be 274.7±6.1 s and no difference was predicted when 1H decoupling was implemented. Using an isotropic rotational correlation time of 25 ps, T1 was 12 s at 8.45 T. The experimentally observed 13C2-DPA singlet state relaxation time at 8.45 T was 288.4±3.7 s without 1H decoupling and 267.1±5.4 s with decoupling between the M2S and S2M. Since n and τ are independent of field strength, spectra and stimulations were repeated at different field strengths. Table 2 shows simulated (i.e., theoretical) and experimental Ts and T1 at various field strengths. Detection occurs on the S2M channel (i.e., for an experiment type that has S2M(1H), detection involves proton signal).







TABLE 2







Theoretical and Experimental T1 and Ts of 13C12-DPA.












Field







Strength
Experiment
Theoretical
Theoretical
Experimental T1
Experimental


(B0 (T))
Type
T1 (13C)
Ts
(13C; 1H)
Ts















8.45
M2S(13C),
12.2 s
274.7 ± 6.1
13C, 13.9 s;
288.4 ± 3.7



S2M(13C)


1H, 3.7 s


8.45
M2S(13C),
—
274.7 ± 6.1
—
267.1 ± 5.4



S2M(13C),



with 1H



decoupling


8.45
M2S(1H),
—
273.2 ± 7.5
—
282.7 ± 3.8



S2M(13C)


16.44
M2S(13C),
 4.6 s
252.0 ± 5.0
13C, 4.9 s;
244.7 ± 1.4



S2M(13C)


1H, 3.8 s


16.44
M2S(13C),
—
276.7 ± 8.5
—
  223 ± 9.1



S2M(1H)


16.44
M2S(1H),
—
 252.5 ± 15.3
—
261.7 ± 7.3



S2M(1H)


1.5
M2S(13C),
29.1 s
309.3 ± 8.2
—



S2M(13C)









It will be understood that various details of the presently disclosed subject matter may be changed without departing from the scope of the presently disclosed subject matter. Furthermore, the foregoing description is for the purpose of illustration only, and not for the purpose of limitation.

## Claims

1. A method of providing a contrast agent for magnetic resonance imaging (MRI) or magnetic resonance spectroscopy, the method comprising:
providing a molecule comprising at least four non-zero-spin nuclei X, X′, Y, and Y′, wherein X and X′ are a first pair of chemically equivalent or effectively equivalent nuclei, and Y and Y′ are a second pair of chemically equivalent or effectively equivalent nuclei, wherein the molecule is selected from the group consisting of diphenylacetylene and diethyl oxalate;
hyperpolarizing the molecule to provide a hyperpolarized molecule; and
applying a first sequence of one or more radiofrequency pulses to the hyperpolarized molecule to transfer a spin state population between the first and the second pair of chemically equivalent or effectively equivalent nuclei; thereby providing a contrast agent having a non-equilibrium singlet state nuclear spin population, wherein the contrast agent molecule can be detected after the application of a second sequence of one or more radiofrequency pulses to transfer singlet order to polarization.

2. The method of claim 1, wherein the hyperpolarizing is performed by dynamic nuclear polarization (DNP).

3. The method of claim 1, wherein the one or more radiofrequency pulses are hydrogen-only pulse sequences.

4. The method of claim 1, further wherein the contrast agent is formulated in a pharmaceutically acceptable carrier and administered to a subject.

5. The method of claim 4, wherein the subject is a mammal.

6. The method of claim 1, wherein the non-equilibrium singlet state nuclear spin population can persist for a time that is greater than about 3 times the longitudinal relaxation time constant (T1).

7. The method of claim 6, wherein the non-equilibrium singlet state nuclear spin population can persist for a time that is greater than about 10 times T1.

8. The method of claim 7, wherein the non-equilibrium singlet state nuclear spin population can persist for a time that is greater than about 50 times T1.

9. A method of imaging a target, the method comprising:
providing a molecule comprising at least four non-zero-spin nuclei X, X′, Y, and Y′, wherein X and X′ are a first pair of chemically equivalent or effectively equivalent nuclei, and Y and Y′ are a second pair of chemically equivalent or effectively equivalent nuclei, wherein the molecule is selected from the group consisting of diphenylacetylene and diethyl oxalate;
hyperpolarizing the molecule to provide a hyperpolarized molecule;
applying a first sequence of one or more radiofrequency pulses to the hyperpolarized molecule to transfer a spin state population between the first and second pair of nuclei, thereby providing a contrast agent having a non-equilibrium singlet state nuclear spin population;
contacting the contrast agent with the target;
applying a second sequence of one or more radiofrequency pulses to convert singlet order to magnetization;
generating a nuclear magnetic resonance signal; and
detecting the nuclear magnetic resonance signal, thereby imaging the target.

10. The method of claim 9, wherein the target is one of a cell, a tissue, an organ, and a subject.

11. The method of claim 9, wherein the contacting comprises administering a pharmaceutical formulation comprising the contrast agent to a subject.

12. The method of claim 11, wherein the subject is a mammal.

13. The method of claim 9, wherein the hyperpolarizing is performed by dynamic nuclear polarization (DNP).

14. The method of claim 8, wherein the non-equilibrium singlet state nuclear spin population can persist for about 69 times T1.

15. The method of claim 9, wherein the one or more radiofrequency pulses of the first and second sequences are hydrogen-only pulse sequences; and wherein the nuclear magnetic resonance signal is a hydrogen signal.