SAT Report

PMN Number: P-13-0270

SAT Date: 2/20/2013

Print Date: 8/30/2013

Related cases:

Health related cases: 

Ecotox related cases: 

Concern levels:

Type of Concern: Health Eco Comments

Level of Concern: 1 3

 Persistence Bioaccum Toxicity Comments

3 11

Exposure Based Review:

Health: No

Ecotox: No

Routes of exposure: Health: Low Concern

Ecotox: All releases to water

Fate: ;

P2Rec Comments:

Comment: Forward
Keywords:

 Keywords: 

POSS IRR-EAQUATOX-A,CSummary of Assessment:

 Fate:

Fate Summary: P-13-0270

FATE: Solid with MP = 111 C (M)log Kow = 6.18 (M) S = 0.004 mg/L at 25 C (M)VP < 1.0E-6 torr at 25 C (M)BP = 418 C (may decompose) (M) H = 8.71E-8 (E)log Koc = 5317 (E)log Fish BCF = (3.75 E)log Fish BAF = 1.60 (E) POTW removal (%) = 90 via sorption; OECD 301F(Mano Resp): 11.5%/28d; OECD 111(Hydrolysis): t1/2(pH4,7,9): >1yr/7.5d/<1d. ICB does not believe the PMN hydrolyzes at pH 7.Time for complete ultimate aerobic biodeg = wk-moSorption to soils/sediments = strong - v.strongPBT Potential: P3B1*CEB FATE: Migration to ground water = negl

 Health:

 Health Summary: Absorption of the neat material is nil through the skin, but poor through th

material in solution, while absorption is poor through the lung and GI tract, based on physical/chemical propertieester hydrolysis. There is possible concern for eye irritation based on submitted test data. However, overall no sconcerns.

 Test Data: Submitted with the PMN:
Negative in Salmonella with and without activation;

Negative in E. coli with and without activation; 

Rat (F) acute (15D) oral (gavage) LD50 > 2000 mg/kg; Rat acute (15D) dermal LD50 > 2000 mg/kg; Mild eye irritant in male rabbits, cleared by 72 hours; Rat repeated dose (28D) oral (diet) toxicity NOAEL 1000 mg/kg, based on increased mean relative liver weights

Not a dermal irritant in male rabbits; Not a demal sensitizer in female mice;, up to 25% ai;Negative for estrogenic potential in immature female rats at 100 mg/kg (highest dose tested; 21D exposure)

 Ecotox:

Test Organism Test Type Test End Point Predicted Measured C

fish 96-h LC50 * *

daphnid 48-h LC50 * * experim

green algal 96-h EC50 * * experim

fish 

_

chronic value 0.001

daphnid _ chronic value 0.008 or *

algal _ chronic value 0.01 or * * experim

Sewage Sludge 3-h EC50 _Sewage Sludge _ Chronic Value _

Ecotox Values Comments: Predictions are based on SARs for esters; SAR chemical class = esters; MW 388

log Kow = 7.2 (EPI); pH7; effective concentrations based on 100% active ingredients and mean measured conc

<180.0 mg/L as CaCO3; and TOC <2.0 mg/L;Ecotoxicity Data Results for Three acute aquatic toxicity tests and an earthworm toxicity test were conducted on P-13-0270. The 96-hour fish was conducted in the T
invertebrate, the 72-hour aquatic plants and the 8 week earthworm toxicity studies were conducted at the Toxico

Environmental Research and Consulting Laboratory of Dow Chemical. All tests were reported to have been conconditions and followed either or a combination of acceptable OECD and EPA guidelines. The PMN substance reported to be 0.004 mg/L. Other submitted data included: white solid; MP = 111 C; BP = 418 C (this may be a temperature; VP = 7.0E-9 torr; log Kow = 7.2 (from ECOSAR version 1.11); density = 1.21 g/cc; not flammablehalf-lives: > 1 year at pH 4, 7.5 days at pH 7 (ICB chemists do not believe this value), and < 1 day at pH 9 (OEC> 5.63 (OECD 121.A 96-hour acute toxicity test was conducted in Chinese rare minnow (Gobiocypris rarus) with P-13-0270 (99.9% conditions. This study was reported to follow OECD test guideline 203. The water solubility of the test substan0.00369 mg/L. Following a range-finding test, a single replicate of ten G. rarus was exposed to a dilution water tap water), a solvent control (0.1 ml/L acetone) or the PMN substance at the nominal test concentration of 100 mgeometric mean measured concentrations of 270 μg/L without centrifugation and 18 μg/L with centrifugation weHPLC analysis (LOD = 5.00 μg/L). The test solution was prepared by adding a slurry of test substance with acet0.1 mL acetone/L) directly to 4 liters of test water in a 5-L glass vessel. The solution was allowed to stir with a mhours with the vortex height of approximately 1/5 of the solution. After stirring, the test solution (without removmaterial) was used directly for testing. During the test, the measured contents of the test substance determined bnot stable in test water since the solubility of the test substance in water is slight and dispersion of the insoluble fnon-homogeneous. The contents of the test substance in test solutions were between 129 

– 1144 μg/L, with a me

mean concentration of 270μg/L. At the same time, although most of the insoluble fractions in test solutions were centrifugation (10000 rpm for 40 min), the contents of the test substance in test solutions were still not stable becinsoluble fractions with contents between 11.3 

– 29.0 μg/L and a measured geometric mean concentration of 18 

course of testing, temperature ranged from 22.8 

– 23.6 ̊C, 

pH ranged fro

m 7.09 

– 7.69 and dissolved oxygen rang

90.3%ASV. Dilution water hardness was 165 mg CaCO3/L. A loading rate of 0.5 g fish/L was calculated. 

No m

abnormalities were observed during the study. The 96-hour LC50 was greater than a nominal test concentration (geometric mean measured concentration of non-centrifuged and centrifuged samples was 270 μg/L and 18 μg/Ltest substance was insoluble with materials present in the solution for both the non-centrifuged and centrifuged tewas not conducted on a standard OPPT fish species. The test was considered inadequate. In addition, the PMN above the reported measured water solubility of 0.004 mg/L.96-hour LC50 = 

NES 

96-hour NOEC = 

NES

A 48-hour acute toxicity study was conducted in Daphnia magna with P-13-0270 (99.85±0.03% 

purity) under sta
study was reported to follow OECD test guideline 202, US EPA FIFRA, 72-2, EPA 540/9-82-020, EEC Directiv

C.2 and US EPA TSCA, 40 CFR 797.1300. The water solubility of the test substance was reported as 0.04 mg/Lrange-finding test, four replicates of five D. magna were exposed to a dilution water control or the PMN substancfraction (WSF) at nominal test concentrations of 6.25, 12.5, 25.0, 50.0 or 100 mg/L. Corresponding mean measu< LLQ (control), 0.000923, 0.00379, 0.0163, 0.0394 and 0.130 mg/L were determined via HPLC/ESI-MS/MS an0.000591 mg/L). The bulk test solution was prepared as a WAF at the highest nominal test concentration (100 mmaterial was weighed out (exact weight = 0.1000 g) directly into a tared scintillation vial. A volumetric flask waof LDW, which was used to thoroughly rinse the contents of the scintillation vial into a 2-L Erlenmeyer flask. Tprepared by pouring 1 L of dilution water into a 2000-mL Erlenmeyer flask. Both flasks were covered with paraseparate stir plates set to stir at approximately 350 rpm. After approximately 24 hours of stirring, ~ 250 mL of teto 250-mL plastic bottles to be centrifuged (4 replicates of control and 2 replicates of the nominal 100 mg/L). Thcentrifuged at ~ 10,000 rpm for approximately 40 minutes. After centrifuging, the control solution was clear and nominal 100 mg/L solution was clear and colorless with some small white particulate suspended. The high-dose diluted with dilution water to create subsequent stock solution for all lower doses in 250-mL glass beakers at the test concentrations. All solutions were clear and colorless. Over the course of testing, temperature ranged from 

from 7.3 

– 7.6 and dissolved oxygen ranged from 8.3 

– 9.2 mg/L. Dilution water hardness ranged from 56 

– 60 m

loading rate of 250 daphnids/L was calculated. Measured concentrations ranged from 0.00747 to 0.0235% of no0.0171 and 0.243% of nominal at 48 hours. At test termination, immobility was observed in 10, 0, 0, 0, 0, and 5% the 0 (control), 0.000923, 0.00379, 0.0163, 0.0394 and 0.130 mg/L test levels, respectively. The 48-hour EC50 wnominal test concentration of 100 mg/L (mean measured concentration of 0.130 mg/L). In addition, the PMN suabove the reported measured water solubility of 0.004 mg/L. The test was considered adequate48-hour EC50 = 

NES

48-hour NOEC = 

NES

A 72-hour limit test was conducted in green algae (Pseudokirchneriella subcapitata) with P-13-0270 (99.85±0.03

conditions. The study was reported to follow OECD test guideline 201 and EEC Directive 92/69/EEC, Part C.3. solubility of the test substance is 0.003 mg/L. Six replicates of P. subcapitata (10,000 cells/mL) were exposed to control or the PMN substance at the nominal test concentration of 100 mg/L. Corresponding mean measured conLOQ (control) and 0.00253 mg/L were determined via HPLC/ESI-MS/MS analysis (LOQ = 0.0000559 mg/L). Areplicate for the AAP control and 100 mg/L treatment were prepared but not inoculated with algae to serve as a calgae were illuminated with a light intensity within the range of 4440 

– 5810 lux with constant shaking. Bulk tes

prepared at nominal concentrations of 0 (water control; AAP) and 100 mg/L. After adding the test substance to tflask was covered with parafilm and left to stir for approximately 24 hours. Preliminary analysis showed that filt
and 0.22 μm filters removed all of the test substance from the solution. Therefore, in an attempt to reduce the am

particles while maximizing exposure concentrations, solutions were centrifuged prior to use. Approximately 250 were added to polypropylene 250-mL bottles which were centrifuged for 45 minutes at approximately 10,000 rpmcarefully decanted into collection vessels which were sampled for analytical confirmation of dose. While centrifremoved much of the particulate matter, some small particulate matter was still visible suspended in the AAP. Tsolutions were shaken vigorously prior to dosing test vessels and prior to analytical sampling to make test solutiopossible. Test vessels were capped with foam stoppers. Over the course of testing, the temperature ranged from ranged from 7.2 – 7.3. Measured concentrations were 0.00227% of nominal at 0 hours and 0.00152-0.00368% oThe mean cell density in the control cultures increased by a factor of 184 within 72 hours. Test substance exposusignificant effect on biomass (p = 0.9094) or growth rate (p = 0.8651); therefore, the 72-hour EC50 and NOEC fgrowth rate were > 0.00253 mg/L and 0.00253 mg/L, respectively, based on mean measured concentrations. The adequate.72-hour EC50 (biomass and growth rate) = NES

72-hour NOEC (biomass and growth rate) = NES

72-hour LOEC (biomass and growth rate) = NES

Conclusion:The acute daphnia and the 72-hour algal tests are considered adquate. The acute fish test was considered inadequ

purposes, based on SAR predictions for esters (ECOSAR version 1.11), the 96-hour fish LC50, 48-hour Daphnid 96-hour algal EC50 were all NES. An earthworm study was submitted for P-13-0270 and appeared to follow staguidelines, but will not be used to set the acute and chronic concentrations of concerns (CoCs). For comparative purposes, ECOSAR version 1.11 using SAR for esters predicted NES for base set aquatic acute fish predicted value was 0.001 mg/L. Chronic data were not submitted for the PMN substance. Based on the resstudies and the reported measured water solubility for each test, the acute CoC for P-13-0270 is NES. The chron(1 ppb) and was derived by dividing the predicted fish ChV (0.001 mg/L) by an assessment factor of 10.Acute CoC = *Chronic CoC = 1 ppb

Factors Values Comments
Assessment Factor

10

Concentration of Concern (ppb) 1

SARs Esters

SAR Class Esters

Ecotox Category Esters

Ecotox Factors Comments: 

 SAT Chair: J. Kwiat