# Patent

## Title
Didemnin biosynthetic gene cluster in Tistrella mobilis

## Abstract

A novel Tistrella mobilis strain having Accession Deposit Number NRRL B-50531 is provided. A method of producing a didemnin precursor, didemnin or didemnin derivative by using the Tistrella mobilis strain, and the therapeutic composition comprising at least one didemnin or didemnin derivative produced from the strain or modified strain thereof are also provided.

## Background
This application is a U.S. §371 national phase filing application from PCT International Application Serial No. PCT/IB2012/002361, filed Sep. 21, 2012, which claims priority to U.S. Provisional Patent Application Ser. No. 61/537,416, filed Sep. 21, 2011, both of which applications are incorporated by reference herein in their entirety.


TECHNICAL FIELD
The present invention generally concerns the fields of cell biology, molecular biology, bacteriology, and medicine. In particular aspects, the invention concerns direct or indirect production of anti-cancer compounds in bacteria.
BACKGROUND OF THE INVENTION
The didemnins (FIG. 1) are a group of cyclic depsipeptides with extraordinary biological activities, including antitumor, antivirus and immunosuppressive activities (Vera and Joullié, 2002; Rawat et al., 2006). Rinehart and his coworkers (1981) initially isolated didemnins A, B and C from a Caribbean tunicate of the family Didemnidae in 1981. Since then, more than 20 other didemnins have been isolated from tunicates collected from different geographical locations. Owing to its potent antitumor activity, didemnin B was the first marine natural product selected to enter clinical trials. Although didemnin B failed in mid-1990s, a closely related didemnin compound-dehydrodidemnin B (Aplidin) is currently in human phase II clinic trials for solid and haematological malignant neoplasias like T cell lymphoma and myelofibrosis and in phase III clinical trials for multiple myeloma (Soto-Matos et al., 2011). Until now, didemnins are exclusively isolated from the eukaryotic marine tunicates. However, since cyclic peptides are usually synthesized by non-ribosomal peptide synthetases (NRPS) from microorganisms, a microorganism associated with the tunicates would be a useful source for producing didemnins. Until the present invention, careful scrutiny of tunicate symbiotic microorganisms has not been successful to locate such a microorganism and chemical synthesis remains to be the only route to obtain sufficient amount of didemnins. The chemical reactions to synthesize didemnin A, the simplest compound of the didemnin family, are too complicated to be finished within 10 steps (Jou et al, 1997), and consequently the yield is undoubtedly very low. Therefore, the discovery of didemnin biosynthetic gene cluster from a microbe will be beneficial for producing didemnins more economically. More importantly, novel didemnin analogues can be generated through genetic engineering of the biosynthetic pathways and be investigated for their biological activities, which may in turn provide more drug leads.
BRIEF SUMMARY OF THE INVENTION
The present invention is directed to a system, method, and compositions related to production of anti-cancer compounds or precursors or derivatives thereto from a bacterium. In particular aspects, the bacterium is from the Tistrella genus, and in specific aspects the bacterium is Tistrella mobilis. 
This invention relates to the production of didemnins or didemnin derivatives through the use of novel didemnin-producing bacterium and/or didemnins or didemnin derivatives produced by these species; it also relates to polynucleotide and amino acids from the novel bacterium producing the didemnins. Furthermore, the invention relates to therapeutic compositions containing the didemnins and to uses of the therapeutic compositions.
It is therefore an object of the present invention to provide novel species of didemnin-producing Tistrella bacteria. It is also an object of the invention to provide a novel Tistrella mobilis bacterium or one or more strains thereof. In specific cases, the novel didemnin-producing Tistrella mobilis bacterium was deposited on Jul. 27, 2011 as Accession No. NRRL B-50531 with the depository Agricultural Research Service Culture Collection, National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Ill. 61604, U.S.A.
An isolated or biologically pure culture of the bacterium is encompassed in the invention. A further object of the present invention is to provide didemnins, didemnin precursors, and/or didemnin derivatives produced by novel strains of Tistrella. In additional aspects of the present invention, there are novel amino acid and polynucleotide sequences of Tistrella mobilis, and exemplary sequences include SEQ ID NOS:10-61.
Any Tistrella species may be utilized in the invention. In specific embodiments, the following bacteria are utilized: Tistrella mobilis (Shi et al., 2002); Tistrella bauzanensis (Zhang et al., 2010), Tistrella sp. BZ78, Tistrella sp. D1-34, Tistrella sp. D1-36, Tistrella sp. D6-30, Tistrella sp. f-1-2, Tistrella sp. JW16.1a, Tistrella sp. MARC2PPND, Tistrella sp. PhS5A, Tistrella sp. S67-5, Tistrella sp. S73-3, and Tistrella sp. Zp5.
Specific embodiments of the invention include the isolation of didemnin B and nordidemnin B from a Gram-negative marine-derived bacterium Tistrella mobilis. The complete genome sequence of this bacterium revealed the biosynthetic gene cluster responsible for the didemnin synthesis. Bioinformatic analysis was used to predict the function of the genes in the cluster. Culture conditions were optimized in order to increase the yield of the target didemnin compounds.
In particular aspects, there is a process for producing a didemnin, which comprises the steps of: a) culturing at least one Tistrella strain in growth-supporting nutrient medium capable of promoting growth and reproduction of said bacteria, wherein said culturing is effected for a time sufficient to allow production of a didemnin; and b) recovering the didemnin from said bacteria or medium of step a).
One embodiment of the present invention is to provide a plurality of bacteria for the mass production of a didemnin or precursor or derivative thereof.
A particular embodiment of the present invention is to provide a novel process for the production of didemnins, didemnin precursors, or didemnin derivatives from bacteria. The industrial application of this process would provide renewable sources of didemnins, didemnin precursors, or didemnin derivatives for the pharmaceutical industry. In certain aspects, there is a biotransformation process in which bacteria-derived didemnins, didemnin precursors, or didemnin derivatives are converted into substances that are useful as therapeutic compounds or for the production of other therapeutic compounds. In certain aspects of the invention, didemnin B and/or nordidemnin B (for example) are produced endogenously by Tistrella mobilis and derivatives are made therefrom one or the other; however, in other cases other didemnins are produced endogenously by other Tistrella bacteria.
Aspects of the invention include methods and compositions wherein bacteria capable of producing didemnin precursors or derivatives, wherein reagents are added to their culture medium to effect production of a didemnin or the didemnin derivatives.
In accordance with the present invention there is also provided a process for improving production of didemnins, didemnin precursors, or didemnin derivatives in bacteria comprising the steps of a) culturing Tistrella bacteria in the presence of a mutagenic agent for a period of time sufficient to allow mutagenesis; and b) selecting said mutants by a change of the phenotype that results in an increased production of didemnins, didemnin precursors, or didemnin derivatives. The mutagenic agent may be a chemical agent, such as daunorubicin and nitrosoguanidine; a physical agent, such as gamma radiation or ultraviolet radiation; or a biological agent, such as a transposon, for example. Exemplary modifications include to the side chain region, to the hip-isostatine region, to the tetrapeptide region, and/or to the macrocyclic backbone.
In certain embodiments, there is provided a process for improving biotransformation of didemnins into didemnin derivative-producing bacteria comprising the steps of a) culturing bacteria in the presence of a mutagenic agent for a time sufficient to allow mutagenesis; and b) selecting said mutants by a change of the phenotype that results in an increased biotransformation of didemnins into didemnin derivative-producing bacteria.
In some aspects of the invention, an anti-cancer compound is produced from Tistrella bacteria. The compound may be isolated directly from the bacteria, or another compound may be isolated from the bacteria from which the anti-cancer compound is then synthesized, either directly or indirectly through one or more other compounds.
Embodiments of the invention include methods and compositions regarding fermentation to produce didemnins and their derivative compounds. Some embodiments include the gene cluster in Tistrella for producing didemnins. Although in specific cases the didemnin gene cluster includes Tistrella didA, didB, didC, didD, didE, didF, didG, didH, and didI; however, in particular embodiments the didemnin gene cluster includes one or more of Tistrella didA, didB, didC, didD, didE, didF, didG, didH, and didI. In at least certain aspects, one or more of the didemnin gene cluster may be transformed into bacteria from another genus, such as Escherichia; in particular, one or more of the didemnin gene cluster members may be transferred into E. coli for the production of a didemnin or didemnin derivative from E. coli, either directly or indirectly.
In particular aspects of the invention, there is included production of didemnins, didemnin precursors, or didemnin derivatives by the Tistrella mobilis JAM 14872T (Shi et al., 2002). Tistrella mobilis JAM 14872T was cultured and extracted by the same way as the Tistrella mobilis strain described herein, and the UPLC-HRMS profile shows that this type strain also produces didemnin B and nordidemnin B.
Anti-cancer compounds of the present invention may be useful for any type of cancer, including at least the following: breast, lung, prostate, colon, pancreatic, blood, brain, liver, spleen, esophageal, ovarian, cervical, kidney, thyroid, rectal, bone, gall bladder, stomach, and so forth. The invention may employed for any type of mammal, including humans, dogs, cats, horses, pigs, sheep, and goats.
In alternative embodiments, the compositions of the invention relate to antiviral and/or immunosuppressive compounds.
In some aspects of the invention, one or more polynucleotides in Tistrella (such as one of plasmids 1, 2, 3, or 4 of Tistrella mobilis, for example) are able to conjugate with other bacteria. In specific cases, the plasmid transfers to E. coli or another bacterium.
In an embodiment of the invention, there is an isolated Tistrella mobilis bacterium having Accession Deposit Number NRRL B-50531 with the depository Agricultural Research Service Culture Collection National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture. In some embodiments, there is an isolated polynucleotide selected from the group consisting of SEQ ID NO:10-25, 42-51, 62-66, and a mixture thereof. In particular embodiments, there is an isolated polypeptide selected from the group consisting of SEQ ID NO:26-41, 52-61, and a mixture thereof.
In specific embodiments, a bacterium comprises at least one genetic modification compared to wild-type, and in certain aspects the genetic modification is in a gene in the didemnin gene cluster. In specific cases, at least one genetic modification is in a condensation, adenylation, thiolation, ketoreductase, ketosynthase, methyltransferase, or thioesterase domain of a gene in the didemnin gene cluster. In certain embodiments, at least one genetic modification is in the ketoreductase or adenylation domains.
In one embodiment of the invention, there is a method of producing a didemnin precursor, didemnin, or didemnin derivative, comprising the steps of a) culturing a host cell harboring bacterial didemnin synthesis genes; and b) recovering said didemnin precursor, didemnin, or didemnin derivative from said host cell. In some cases, the host cell is further defined as a Tistrella bacterium, a genetically modified Tistrella bacterium compared to wild-type, or E. coli. The Tistrella bacterium may be Tistrella mobilis, in some cases. In particular aspects, the didemnin is selected from the group consisting of didemnin A, didemnin B, didemnin C, didemnin D, didemnin E, didemnin G, didemnin X, didemnin Y, nordidemnin, or a combination thereof. In some cases, the method further comprises modifying the recovered didemnin precursor, didemnin, or didemnin derivative.
In an embodiment of the invention, there is a therapeutic composition comprising in a suitable carrier: at least one isolated didemnin produced by a culture comprising bacteria having Accession Deposit Number NRRL B-50531 with the depository Agricultural Research Service Culture Collection National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture; at least one didemnin or didemnin derivative produced from a modified bacteria of the bacterial strain having Accession Deposit Number NRRL B-50531 with the depository Agricultural Research Service Culture Collection National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture; or a mixture thereof.
The foregoing has outlined rather broadly the features and technical advantages of the present invention in order that the detailed description of the invention that follows may be better understood. Additional features and advantages of the invention will be described hereinafter which form the subject of the claims of the invention. It should be appreciated by those skilled in the art that the conception and specific embodiment disclosed may be readily utilized as a basis for modifying or designing other structures for carrying out the same purposes of the present invention. It should also be realized by those skilled in the art that such equivalent constructions do not depart from the spirit and scope of the invention as set forth in the appended claims. The novel features which are believed to be characteristic of the invention, both as to its organization and method of operation, together with further objects and advantages will be better understood from the following description when considered in connection with the accompanying figures. It is to be expressly understood, however, that each of the figures is provided for the purpose of illustration and description only and is not intended as a definition of the limits of the present invention.



BRIEF DESCRIPTION OF THE DRAWINGS
For a more complete understanding of the present invention, reference is now made to the following descriptions taken in conjunction with the accompanying drawings.
FIGS. 1A and 1B illustrate structures of didemnin B and its exemplary monomers and other representative didemnins.
FIG. 2 provides detection of didemnin B (upper panel) and nordidemnin B (lower panel) in one of the fractions of Tistrella mobilis crude extract using UPLC-HRMS analysis.
FIG. 3 shows organization of didemnin NRPS-PKS system.
FIG. 4 illustrates an exemplary incorporation of the two monomers Ist and Hip into didemnin B. Reactions are simplified by taking out the thiol-tethered peptidyl intermediates.
FIG. 5 shows an exemplary biosynthetic pathway of didemnin B. C: condensation domain. A: adenylation domain. T: thiolation domain. KR: ketoreductase domain. KS: ketosynthase domain. MT: methyl transferase domain. TE: thioesterase domain.
FIG. 6 shows detection of didemnin A from Tistrella mobilis. 
FIGS. 7A and 7B show detection of didemnin N and nordidemnin Afrom Tistrella mobilis. 
FIGS. 8A and 8B show detection of two new didemnins from Tistrella mobilis. 



DETAILED DESCRIPTION OF THE INVENTION
As used herein the specification, “a” or “an” may mean one or more. As used herein in the claim(s), when used in conjunction with the word “comprising”, the words “a” or “an” may mean one or more than one. As used herein “another” may mean at least a second or more. In specific embodiments, aspects of the invention may “consist essentially of” or “consist of” one or more sequences of the invention, for example. Some embodiments of the invention may consist of or consist essentially of one or more elements, method steps, and/or methods of the invention. It is contemplated that any method or composition described herein can be implemented with respect to any other method or composition described herein. Embodiments discussed in the context of methods and/or compositions of the invention may be employed with respect to any other method or composition described herein. Thus, an embodiment pertaining to one method or composition may be applied to other methods and compositions of the invention as well.
I. Definitions
The definitions provided in the entire disclosure supersede any conflicting definition in any of the reference that is incorporated by reference herein. The fact that certain terms are defined, however, should not be considered as indicative that any term that is undefined is indefinite. Rather, all terms used are believed to describe the invention in terms such that one of ordinary skill can appreciate the scope and practice the present invention.
The term “didemnin” as used herein refers to a group of cyclic depsipeptides. In specific embodiments, the didemnin has antitumor, antiviral, and/or immunosuppressive activity.
The term “didemnin derivatives” as used herein refers to any molecule constructed based on modification of a didemnin compound. In specific embodiments, the didemnin derivatives have antitumor, antiviral, and/or immunosuppressive activity.
The term “didemnin gene cluster” as used herein refers to a cluster of genes responsible for the biosynthesis of didemnins.
“Effective amount,” “Therapeutically effective amount” or “pharmaceutically effective amount” means that amount which, when administered to a subject or patient for treating a disease, is sufficient to effect such treatment for the disease, including to improve at least one symptom of the disease.
The term “growth supporting nutrient medium” is intended to mean any culture media which include, without limitation, carbon sources, nitrogen sources, amino acids, vitamins and minerals.
As used herein, the term “patient” or “subject” or “individual” refers to a living mammalian organism, such as a human, monkey, cow, sheep, goat, dog, cat, mouse, rat, guinea pig, or transgenic species thereof. In certain embodiments, the patient or subject is a primate. Non-limiting examples of human subjects are adults, juveniles, infants and fetuses.
“Prevention” or “preventing” includes: (1) inhibiting the onset of a disease in a subject or patient that may be at risk and/or predisposed to the disease but does not yet experience or display any or all of the pathology or symptomatology of the disease, and/or (2) slowing the onset of the pathology or symptoms of a disease in a subject or patient that may be at risk and/or predisposed to the disease but does not yet experience or display any or all of the pathology or symptoms of the disease. An individual at risk for cancer, for example, may be an individual with a family or personal history or that exhibits one or more known risk factors, such as certain lifestyle habits (for example, smoking) or particular gene-associated mutations (for example, BRCA1 or BRCA2 for breast or ovarian cancer) or particular elevations of a metabolite (elevated prostate-specific antigen (PSA) for prostate cancer).
II. General Embodiments of the Invention
In embodiments of the invention, there are bacteria that produce didemnins or didemnin derivatives, and in particular aspects the bacteria are from the family Rhodospirillales, although in specific aspects the bacteria are from the genus Tistrella. 
The present invention at least provides a novel Tistrella mobilis bacterium; amino acid and polynucleotide sequences of the bacterium; didemnins, didemnin precursors, and/or didemnin derivatives produce therefrom; therapeutic compositions containing the didemnins, didemnin precursors, and/or didemnin derivatives; and/or methods for producing or using the didemnins, didemnin precursors, and/or didemnin derivative compositions.
III. Tistrella Mobilis Sequences and the Didemnin Gene Cluster
Embodiments of the invention include isolated polynucleotide and polypeptide sequences from Tistrella mobilis, such as those included in SEQ ID NOS:10-61. In some cases, the isolated polynucleotide and polypeptide sequences from Tistrella mobilis are involved in synthesis of one or more didemnins, whereas in other cases the isolated polynucleotide and polypeptide sequences from Tistrella mobilis are not involved in synthesis of one or more didemnins. The isolated polynucleotide or polypeptide sequences may be modified and utilized in a cell or in vitro. In certain cases, the modified polynucleotide or polypeptide sequences are utilized in a Tistrella mobilis or other bacterial or yeast cell. Isolated or modified polynucleotide sequences may be employed in an expression vector.
Tistrella mobilis gene cluster nucleotide sequences include ORF1 (SEQ ID NO:10), ORF2 (SEQ ID NO:11), ORF3 (SEQ ID NO:12), ORF4 (SEQ ID NO:13), ORF5 (SEQ ID NO:14), ORF6 (SEQ ID NO:15), ORF7 (SEQ ID NO:16), ORF8 (SEQ ID NO:17), ORF9 (SEQ ID NO:18), ORF10 (SEQ ID NO:19), ORF11 (SEQ ID NO:20), ORF12 (SEQ ID NO:21), ORF13 (SEQ ID NO:22), ORF14 (SEQ ID NO:23), ORF15 (SEQ ID NO:24), and ORF16 (SEQ ID NO:25). Corresponding Tistrella mobilis gene cluster protein sequences for the respective ORFS is as follows: ORF1 (SEQ ID NO:26), ORF2 (SEQ ID NO:27), ORF3 (SEQ ID NO:28), ORF4 (SEQ ID NO:29), ORF5 (SEQ ID NO:30), ORF6 (SEQ ID NO:31), ORF7 (SEQ ID NO:32), ORF8 (SEQ ID NO:33), ORF9 (SEQ ID NO:34), ORF10 (SEQ ID NO:35), ORF11 (SEQ ID NO:36), ORF12 (SEQ ID NO:37), ORF13 (SEQ ID NO:38), ORF14 (SEQ ID NO:39), ORF15 (SEQ ID NO:40), and ORF16 (SEQ ID NO:41).
Tistrella mobilis didemnin gene cluster nucleotide sequences include one or more of the following: DidA (SEQ ID NO:42); DidB (SEQ ID NO:43), DidC (SEQ ID NO:44), DidD (SEQ ID NO:45), DidE (SEQ ID NO:46), DidF (SEQ ID NO:47), DidG (SEQ ID NO:48), DidH (SEQ ID NO:49), DidI, (SEQ ID NO:50), and DidJ (SEQ ID NO:51). Corresponding Tistrella mobilis didemnin gene cluster protein sequences include one or more of the following: DidA (SEQ ID NO:52); DidB (SEQ ID NO:53), DidC (SEQ ID NO:54), DidD (SEQ ID NO:55), DidE (SEQ ID NO:56), DidF (SEQ ID NO:57), DidG (SEQ ID NO:58), DidH (SEQ ID NO:59), DidI, (SEQ ID NO:60), and DidJ (SEQ ID NO:61).
The whole Tistrella mobilis genome is provided by a chromosomal sequence (SEQ ID NO:62) and four mini-chromosomes (which may be referred to as plasmids) including plasmid 1 (SEQ ID NO:63), plasmid 2 (SEQ ID NO:64), plasmid 3 (SEQ ID NO:65), and plasmid 4 (SEQ ID NO:66).
In aspects of the invention, the endogenous Tistrella mobilis didemnin gene cluster includes polypeptides that are modular according to the particular domains they employ. They may have one or more of the following domains (one or more) adenylation (A) domain, the thiolation (T) domain, the condensation (C) domain, the ketoreductase (KR) domain, and/or the methyltransferase (MT) domain, for example. For example, didI has a C domain, an A domain, and a T domain, whereas didB has a C, A, T, and KR domain (see FIG. 3). Marahiel et al. (1997) provide a review of peptide synthetases, which is incorporated by reference herein in its entirety.
IV. Didemnins
Didemnins are cyclic depsipeptide compounds (depsipeptide is a peptide in which one or more of the amide (—CONHR—) bonds are replaced by ester (COOR) bonds). Although more than nine didemnins (didemnins A-E, G, X and Y) have been isolated from the extract of the exemplary tunicate Trididemnum solidum, in the prior art didemnin B is the one that possesses the most potent biological activities. It is a strong antiviral agent against both DNA and RNA viruses such as herpes simplex virus type 1, a strong immunosuppressant that shows some potential in skin graft and is also very cytotoxic. Although didemnin B shows strong activity against murine leukemia cells and had completed phase II human clinical trials against adenocarcinoma of the kidney, advanced epithelial ovarian cancer, and metastatic breast cancer, it exhibits high toxicity in human subjects. For review see Vera and Joullié, 2002.
Didemnin precursors, didemnins, and didemnin derivatives are encompassed in methods and compositions of the invention. Exemplary didemnins that may be generated in the T. mobilis bacterium of the invention may be of any kind, but in specific embodiments they are didemnins A-I, M, X and Y, nordidemnin B, dehydrodidemnin B (Aplidine®), or are one or more of the didemnins described in U.S. Pat. No. 5,294,603, incorporated by reference herein in its entirety. In specific embodiments, didemnin derivatives include N-acyl congeners of didemnin A (DA); several DDB-type analogues of DA in which either pyruvic acid has been replaced (with phenylpyruvic acid or alphaketobutyric acid) or proline at position 8 has been replaced [with L-azetidine-2-carboxylic acid (AZT), L-pipecolic acid (Pip), 1-amino-1-carboxylic cyclopentane (acc5), D-Pro or sarcosine (sar); and the didemnins—X [(R)-3-hydroxy-decanoyl-(Gln)3-Lac-Pro didemnin A]; Y [(R)-3-hydroxy-decanoyl-(Gln)4-Lac-Pro didemnin A]; M (pGlu-Gln-Lac-Pro-didemnin A); N ([Tyr5] didemnin B); nordidemnin N ([Tyr5] nordidemnin B); and epididemnin A ([2S,4R-Hip2] didemnin A). Others include Isodidemnin A1, Didemnin N, Nordidemnin N, Epididemnin A, Acyclodidemnin A, Dihydrodidemnin N, Dihydroepididemnin A, N-Acetyl didemnin A, N,O-Diacetyldidemnin A, N-(D-Prolyl) didemnin A, N-(benzyloxycarbonyl-D-prolyl) didemnin A, N-(D-Prolyl) didemnin A, N-(L-Prolyl) didemnin A, Acetyl9-didemnin B, Propionyl9-didemnin B, Isobutyryl9-didemnin B, L-Ala8-didemnin B, O-Benzyl[L-Ala8]didemnin B, L-Ala8]didemnin B, [D-Pro8]didemnin B, O-Benzyl-[D-pro8]didemnin B, [D-Proo]didemnin B, [N—(CH3ONsu)didemnin A, O-Acetyldidemnin A, [HexahydroMe2 Tyr5]didemnin A, [Hexahydro-N-MePhe5]didemnin A, [HexahydroMe2 Tyr5]didemnin B, [Hexahydro-N-MePhe5]didemnin B, pyroglutaminyl didemnin B.
Glutaminyl derivatives as described in U.S. Pat. No. 6,841,530, incorporated by reference herein in its entirety, are also encompassed in the invention. Examples of derivatives include pyroglutaminyl didemnin B, O-Benzyldidemnin B, Benzyloxycarbonyl-L-Glutaminyldidemnin B, (Benzyloxycarbonyl-L-Glutaminy)2 Didemnin, Benzyloxycarbonyldidemnin M, Benzyloxycarbony-L-Pyroglutaminyldidemnin B, Pyroglutaminyldidemnin B, Prolydidemnin A, L-(N-Benzyloxycarbonyl-pyroglutaminyl)-L-glutaminyl-didemnin B, N-Benzyloxycarbonyl-L-pyroglutaminyl-didemnin B, Boc-L-prolyl-didemnin A, L-Prolyl-didemnin B (see also US2001/0007855, incorporated by reference herein in its entirety).
In specific aspects, the didemnin is an N-acylated Didemnin A. In particular aspects of the invention, the acyl group of the N-acyl Didemnin A comprises a C3 to C8 group. In certain embodiments, the didemnin is a derivative of Didemnin A (optionally a synthetic derivative), modified at a position selected from the group consisting of position 1, 5, 6, and combinations thereof, by the incorporation of a D-amino acid (for example). In other aspects, the didemnin is a synthetic derivative of Didemnin A, modified to include a Dehydrodidemnin B (DDB) moiety in the linear peptide chain. In particular aspects, the didemnin is selected from the group consisting of phenylpyruv-Pro didemnin A, Pyruv-Sar didemnin A, alpha-ketobutyryl-Pro didemnin A, Pyruv-Azt didemnin A, or Pyruv-D-Pro didemnin A.
In some aspects of the invention, one increases the lipophilicity of a didemnin, such as didemnin A, in its modifications, for example to raise its solubility in the plasma membrane and in at least certain cases increase its activity. In specific aspects, the N-amine of N-methyl D-leucine positions is useful for the addition of hydrophobic groups to didemnin A, for example. From here, one can synthesize a series of N-acylated analogues of didemnin A comprising alkyl chains with 2, 3, 4, 5, 7, 11, 15 or 17 carbon atoms.
Dehydrodidemnin B derivatives which either pyruvic acid has been replaced (with phenylpyruvic acid or -ketobutyric acid, for example) or proline at position 8 has been replaced (with L-azetidine-2-carboxylic acid (AZT), L-pipecolic acid (Pip), 1-amino-1-carboxylic cyclopentane(acc5), D-Pro or sarcosine (sar), for example).
Exemplary hydrophobic derivatives of didemnin A may be synthesized by incorporating acyl chains therein didemnin A, ranging from 4 to 18 carbons, for example. Such compounds include N-butyryl didemnin A, N-pentanoyl didemnin A, N-hexanoyl didemnin A, N-octanoyl didemnin A, N-lauroyl didemnin A, N-palmitoyl didemnin A, or N-stearoyl didemnin A.
Three other exemplary derivatives of didemnin A include those in which the amino acids at positions 1, 5, and 6 were replaced with their corresponding D-amino acids. Exemplary compounds include D-Thr1 didemnin A, D-Pro5] didemnin A, and D-MeTyr(Me)6] didemnin A.
Five didemnin A derivatives related to dehydrodidemnin B may be synthesized by introducing DDB-type modifications into their linear peptide chain moieties. Such DDB-type compounds include Phenylpyruv-Pro didemnin A, Pyruv-Sar didemnin A-ketobutyryl-Pro didemnin A, Pyruv-Azt didemnin A, and Pyruv-D-Po didemnin A.
Synthesis of N-acyl analogues may be carried out in solution. Acyl groups may be introduced at the N-methyl-D-leucine unit of didemnin A using a symmetrical anhydride procedure with C6 to C18 fatty acids, and an excess of symmetrical anhydride may be required in order to achieve complete acylation. Propionic butyric and pentanoic acids may be introduced using a 3-5 fold excess of their symmetrical anhydride.
The symmetrical anhydrides of fatty acids may be prepared in the conventional manner using EDC. Acylation of didemnin A may be carried out in the presence of a catalytic amount of dimethylamino pyridine (DMAP). Derivatives may be purified on a silica gel column using methanol/chloroform as a eluant. They and others may be characterized using 1H NMR spectroscopy and HRFABMS. Exemplary methods of synthesis of didemnin analogs is described in U.S. Pat. No. 5,294,603, which is incorporated by reference herein in its entirety.
Structure elucidation, chemical conversion, biological activities including cytotoxicity, antiviral and immunosuppressive activities and structure-activity relationships may be performed for any didemnin or didemnin derivative of the invention.
V. Production of Modified Organisms for Didemnin Precursors, Didemnins, and/or Didemnin Derivatives, and Compositions Produced Thereby
In some embodiments of the invention, one or more host cells (which may be organisms) are modified to produce one or more didemnin precursors, didemnins, or didemnin derivatives. In some cases the organism is from the Tistrella genus, such as Tistrella mobilis, for example, although in other cases an organism or other cells are modified to produce didemnin precursors, didemnins, or didemnin derivatives.
A. Tistrella 
In certain embodiments of the invention, a Tistrella bacterium is modified to affect the synthesis of one or more endogenous didemnins produced in the native bacterium. In some cases, the modified bacterium is genetically modified (such as by using recombinant technology to mutate or knock out one or more endogenous bacteria genes, including by transforming the bacteria with a polynucleotide construct, for example), although it may also or otherwise be chemically modified (daunorubicin or nitrosoguanidine, for example) and/or physically modified (gamma or ultraviolet radiation, for example).
For genetic modification of Tistrella (or other organisms), nucleic acid molecules comprising a mutation of interest or means to generate a mutation of interest) of the present invention can be expressed separately, i.e., inserted into separate vectors for expression. Such vectors are known or can be constructed by those skilled in the art and generally contain all expression elements (e.g., promoters, terminator fragments, enhancer elements, marker genes and other elements as appropriate) necessary to achieve the desired transcription of the sequences. Examples of vectors include viruses such as bacteriophages, baculoviruses, and retroviruses, DNA viruses, cosmids, plasmids, phagemids and other recombination vectors. The vectors can also contain elements for use in either prokaryotic or eukaryotic host systems. One of ordinary skill in the art will know which host systems are compatible with a particular vector. The vectors can be introduced into cells or tissues and expressed by any one of a variety of known methods within the art. Such methods can be found generally described in Sambrook et al. (1989, 1992) Molecular Cloning: A Laboratory Manual, Cold Springs Harbor Laboratory, New York; Ausubel et al. (1989) Current Protocols in Molecular Biology, John Wiley and Sons, Baltimore, Md.; Chang, et al. (1995) Somatic Gene Therapy, CRC Press, Ann Arbor, Mich.; Vega, et al. (1995) Gene Targeting, CRC Press, Ann Arbor, Mich.; Vectors: A Survey of Molecular Cloning Vectors and Their Uses, Butterworths, Boston, Mass. (1988); and include, for example, stable or transient transfection, lipofection, electroporation and infection with recombinant viral vectors. Introduction of nucleic acids by infection offers several advantages over other listed methods. Higher efficiencies can be obtained due to their infectious nature. Moreover, viruses are very specialized and typically infect and propagate in specific cell types. Thus, their natural specificity can be used to target the vectors to specific cell types in vivo or within a tissue or mixed culture of cells. The viral vectors can also be modified with specific receptors or ligands to alter target specificity through receptor mediated events.
B. Non-Tistrella Cells
Host cells suitable for introduction and expression of the nucleic acids of the invention may be bacterial; however, yeast (e.g., Pichia, Saccharomyces, etc.), mammalian, or insect host cells are also contemplated, as is a cell-free expression system. In particular embodiments, the host cell or culture is bacterial. Exemplary bacterial host cells include E. coli as well as Bacillus sp.
In cases wherein Tistrella is not used as a fermentation or other system for production of modified didemnin and didemnin-related compounds, the host cell may be modified to include one or more proteins suitable for production of at least one didemnin precursor, didemnin, and/or didemnin derivative. In particular, the host cell may be modified to include part or all of a didemnin gene cluster, including one or more of didA-didJ and/or ORF1-16, for example. In specific embodiments, the cell includes ORF1, ORF3, ORF6, ORF 7, ORF 8 and/or ORF 16. In particular aspects, one or more of ORF1, ORF3, ORF6, ORF 7, ORF 8 and/or ORF 16 are involved in the regulation, transport, resistance and other functions of the didemnin gene cluster. In some cases, the host cell may be modified to include a mutated did gene (compared to Tistrella wildtype sequence) such that a didemnin derivative may be produced, and it optionally may also include one or more of the endogenous Tistrella wild-type did genes. The didemnin derivative may be produced directly therefrom the modified host cell, or a didemnin precursor may be produced and additional synthesis steps may be required following purification of the precursor, for example.
C. Exemplary Modifications of Host Cells
In particular aspects of the invention, a host cell (Tistrella or E. coli, for example) is modified compared to their respective wild-type counterparts. The modification may be such that one or more endogenous polynucleotides in the host cell become mutated compared to their respective wild-type counterparts. The mutation may be of any kind, including a knockout, knockdown, point, frameshift, inversion, deletion, and so forth. The mutation may be of any kind so as to effect an altered synthesis of one or more of a didemnin precursor, didemnin, or didemnin derivative.
In some cases, one or more mutations are generated in one or more didemnin gene cluster members, including one or more of didA-didJ, and/or in one or more of ORF1-ORF16. In certain cases, a particular region of one or more didemnin gene cluster members is mutated. The region may be of any kind so as to effect an altered synthesis of one or more of a didemnin precursor, didemnin, or didemnin derivative. The affected region may be in the sequence that encodes (one or more) adenylation (A) domain, the thiolation (T) domain, the condensation (C) domain, the ketoreductase (KR) domain, and/or the methyltransferase (MT) domain, for example.
In particular aspects the A domain is modified, for example such that the A domain specificity and/or placement within the non-ribosomal peptide synthetase assembly affects the sequence of the monomers in the nonribosomal peptide. In specific cases, the particular DidA-DidJ genes is modified such that the substrate specificity is different. For example, the DidA A domain may be altered to have substrate specificity other than glutamine, DidC may be altered to have substrate specificity other than proline, and so forth (see Table 3 of predicted normal substrate specificities).
In particular cases, one can genetically modify one or more genes of the didemnin gene cluster such that the pathway generates a didemnin derivative or didemnin precursor that can then be modified following purification of the precursor. For example, one can knock out one or more genes or one or more regions of one or more genes. For example, one can knock out the ketoreductase in DidB so that the pathway produces dehydrodidemnin B. In other exemplary measures, one can replace one of the A domains with another A domain in any of the members of the gene cluster, for example. For example, one can replace one of the A domains with another A domain (for example, one that activate Valine) such that a different didemnin with a Valine residue will be produced.
In some cases, the mutation is generated in the host cell. Following this, the mutant strain can be cultured to produce the desired didemnin precursor, didemnin, or didemnin derivative, and the compound can then be purified (for example, by one or more of extraction, fractionation by normal or reverse phase liquid chromatography, size-exclusion chromatography, reverse-phase HPLC, and so forth). If appropriate, further modifications to the compound may be produced. In some cases, one can culture the mutant strain and wild-type strain and extract them respectively; then, one can compare their respective metabolites, for example by LC-HRMS (high resolution mass spectrometry) and/or NMR to identify or verify production of the expected metabolite.
In alternative embodiments, one can mutagenize a plurality of host cells (including Tistrella or E. coli) for example and performs a high throughput assay to obtain the mutant. The candidate mutants producing didemnin precursors, didemnins, or didemnin derivatives may be screened for a compound not otherwise obtained from the corresponding wild-type host cell or obtainable to much higher levels than in the corresponding wildtype host cell.
VI. Determination of Didemnin or Didemnin Derivative Structure
In embodiments of the invention, the didemnin or didemnin derivative is isolated and the structure is determined. Although one of skill in the art recognizes routine methods of purifying and determining a chemical structure, in specific cases one may use extraction (including, for example, methanol-toluene (or ethyl acetate or chloroform) extraction), silica gel, preparative thin-layer chromatrography, nuclear magnetic resonance imaging, acid hydrolysis, mass spectrometry, gas chromatography, x-ray crystallography, and a combination thereof.
VII. Exemplary Synthesis Embodiments
In certain cases, a didemnin precursor, didemnin, and/or didemnin derivative compounds is obtained by bacteria or other fermentation methods of the invention and is then processed. The processing may include further purification, although in some embodiments the compound is subject to one or more further synthesis steps to obtain the desired molecule. Any suitable further synthesis steps may be employed in the art and are known to the skilled artisan (for representative examples, see Mayer et al., (1994); Jou et al. (1997)).
In specific aspects of the invention, there is in vitro conversion of didemnin B from T. mobilis to dehydrodidemnin B, for example using an oxidizing agent to oxidize the didemnin B to dehydrodidemnin B (see Faulkner D J. Marine pharmacology. (2000) Antonie van Leeuwenhoek 77: 135-145, incorporated by reference herein in its entirety).
Thus, compounds of the present disclosure may be made using the methods known in the art. These methods can be further modified and optimized using the principles and techniques of organic chemistry as applied by a person skilled in the art. Such principles and techniques are taught, for example, in March's Advanced Organic Chemistry: Reactions, Mechanisms, and Structure (2007), which is incorporated by reference herein in its entirety.
Compounds encompassed in methods of the invention or produced directly or indirectly there by may contain one or more asymmetrically-substituted carbon or nitrogen atoms, and may be isolated in optically active or racemic form. Thus, all chiral, diastereomeric, racemic form, epimeric form, and all geometric isomeric forms of a structure are intended, unless the specific stereochemistry or isomeric form is specifically indicated. Compounds may occur as racemates and racemic mixtures, single enantiomers, diastereomeric mixtures and individual diastereomers. In some embodiments, a single diastereomer is obtained. The chiral centers of the compounds of the present invention can have the S or the R configuration.
Compounds of the invention may also have the advantage that they may be more efficacious than, be less toxic than, be longer acting than, be more potent than, produce fewer side effects than, be more easily absorbed than, and/or have a better pharmacokinetic profile (e.g., higher oral bioavailability and/or lower clearance) than, and/or have other useful pharmacological, physical, or chemical properties over, compounds known in the prior art, whether for use in the indications stated herein or otherwise.
In addition, atoms making up the compounds of the present invention are intended to include all isotopic forms of such atoms. Isotopes, as used herein, include those atoms having the same atomic number but different mass numbers. By way of general example and without limitation, isotopes of hydrogen include tritium and deuterium, and isotopes of carbon include 13C and 14C.
It should be recognized that the particular anion or cation forming a part of any salt of this invention is not critical, so long as the salt, as a whole, is pharmacologically acceptable. Additional examples of pharmaceutically acceptable salts and their methods of preparation and use are presented in Handbook of Pharmaceutical Salts: Properties, and Use (2002), which is incorporated herein by reference.
When used in the context of a chemical group, “hydrogen” means —H; “hydroxy” means —OH; “oxo” means ═O; “halo” means independently —F, —Cl, —Br or —I; “amino” means —NH2 (see below for definitions of groups containing the term amino, e.g., alkylamino); “hydroxyamino” means —NHOH; “nitro” means —NO2; imino means ═NH (see below for definitions of groups containing the term imino, e.g., alkylimino); “cyano” means —CN; “isocyanate” means —N═C═O; “azido” means —N3; in a monovalent context “phosphate” means —OP(O)(OH)2 or a deprotonated form thereof; in a divalent context “phosphate” means —OP(O)(OH)O— or a deprotonated form thereof; “mercapto” means —SH; “thio” means ═S; “thioether” means —S—; “sulfonamido” means —NHS(O)2— (see below for definitions of groups containing the term sulfonamido, e.g., alkylsulfonamido); “sulfonyl” means —S(O)2— (see below for definitions of groups containing the term sulfonyl, e.g., alkylsulfonyl); and “sulfinyl” means —S(O)— (see below for definitions of groups containing the term sulfinyl, e.g., alkylsulfinyl).
In the context of chemical formulas, the symbol “—” means a single bond, “═” means a double bond, and “≡” means triple bond. The symbol “————” represents an optional bond, which if present is either single or double. The symbol “” represents a single bond or a double bond. Thus, for example, the structure




includes the structures





As will be understood by a person of skill in the art, no one such ring atom forms part of more than one double bond. The symbol “”, when drawn perpendicularly across a bond indicates a point of attachment of the group. It is noted that the point of attachment is typically only identified in this manner for larger groups in order to assist the reader in rapidly and unambiguously identifying a point of attachment. The symbol “” means a single bond where the group attached to the thick end of the wedge is “out of the page.” The symbol “” means a single bond where the group attached to the thick end of the wedge is “into the page”. The symbol “” means a single bond where the conformation (e.g., either R or S) or the geometry is undefined (e.g., either E or Z).

Any undefined valency on an atom of a structure shown in this application implicitly represents a hydrogen atom bonded to the atom. When a group “R” is depicted as a “floating group” on a ring system, for example, in the formula:




then R may replace any hydrogen atom attached to any of the ring atoms, including a depicted, implied, or expressly defined hydrogen, so long as a stable structure is formed. When a group “R” is depicted as a “floating group” on a fused ring system, as for example in the formula:




then R may replace any hydrogen attached to any of the ring atoms of either of the fused rings unless specified otherwise. Replaceable hydrogens include depicted hydrogens (e.g., the hydrogen attached to the nitrogen in the formula above), implied hydrogens (e.g., a hydrogen of the formula above that is not shown but understood to be present), expressly defined hydrogens, and optional hydrogens whose presence depends on the identity of a ring atom (e.g., a hydrogen attached to group X, when X equals —CH—), so long as a stable structure is formed. In the example depicted, R may reside on either the 5-membered or the 6-membered ring of the fused ring system. In the formula above, the subscript letter “y” immediately following the group “R” enclosed in parentheses, represents a numeric variable. Unless specified otherwise, this variable can be 0, 1, 2, or any integer greater than 2, only limited by the maximum number of replaceable hydrogen atoms of the ring or ring system.
For the groups and classes below, the following parenthetical subscripts further define the group/class as follows: “(Cn)” defines the exact number (n) of carbon atoms in the group/class. “(C≦n)” defines the maximum number (n) of carbon atoms that can be in the group/class, with the minimum number as small as possible for the group in question, e.g., it is understood that the minimum number of carbon atoms in the group “alkenyl(C≦8)” or the class “alkene(C≦8)” is two. For example, “alkoxy(C≦10)” designates those alkoxy groups having from 1 to 10 carbon atoms (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, or any range derivable therein (e.g., 3 to 10 carbon atoms). (Cn-n′) defines both the minimum (n) and maximum number (n′) of carbon atoms in the group. Similarly, “alkyl(C2-10)” designates those alkyl groups having from 2 to 10 carbon atoms (e.g., 2, 3, 4, 5, 6, 7, 8, 9, or 10, or any range derivable therein (e.g., 3 to 10 carbon atoms)).
The term “saturated” as used herein means the compound or group so modified has no carbon-carbon double and no carbon-carbon triple bonds, except as noted below. The term does not preclude carbon-heteroatom multiple bonds, for example a carbon oxygen double bond or a carbon nitrogen double bond. Moreover, it does not preclude a carbon-carbon double bond that may occur as part of keto-enol tautomerism or imine/enamine tautomerism.
The term “aliphatic” when used without the “substituted” modifier signifies that the compound/group so modified is an acyclic or cyclic, but non-aromatic hydrocarbon compound or group. In aliphatic compounds/groups, the carbon atoms can be joined together in straight chains, branched chains, or non-aromatic rings (alicyclic). Aliphatic compounds/groups can be saturated, that is joined by single bonds (alkanes/alkyl), or unsaturated, with one or more double bonds (alkenes/alkenyl) or with one or more triple bonds (alkynes/alkynyl). When the term “aliphatic” is used without the “substituted” modifier only carbon and hydrogen atoms are present. When the term is used with the “substituted” modifier one or more hydrogen atom has been independently replaced by one of the following exemplary non-limiting functional groups: —OH, —F, —Cl, —Br, —I, —NH2, —NO2, —CO2H, —CO2CH3, —CN, —SH, —OCH3, —OCH2CH3, —C(O)CH3, —N(CH3)2, —C(O)NH2, —B(OH)2, —P(O)(OCH3)2 or —OC(O)CH3.
The term “alkyl” when used without the “substituted” modifier refers to a monovalent saturated aliphatic group with a carbon atom as the point of attachment, a linear or branched, cyclo, cyclic or acyclic structure, and no atoms other than carbon and hydrogen. Thus, as used herein cycloalkyl is a subset of alkyl. The groups —CH3 (Me), —CH2CH3 (Et), —CH2CH2CH3 (n-Pr), —CH(CH3)2 (iso-Pr), —CH(CH2)2 (cyclopropyl), —CH2CH2CH2CH3 (n-Bu), —CH(CH3)CH2CH3 (sec-butyl), —CH2CH(CH3)2 (iso-butyl), —C(CH3)3 (tert-butyl), —CH2C(CH3)3 (neo-pentyl), cyclobutyl, cyclopentyl, cyclohexyl, and cyclohexylmethyl are non-limiting examples of alkyl groups. The term “alkanediyl” when used without the “substituted” modifier refers to a divalent saturated aliphatic group, with one or two saturated carbon atom(s) as the point(s) of attachment, a linear or branched, cyclo, cyclic or acyclic structure, no carbon-carbon double or triple bonds, and no atoms other than carbon and hydrogen. The groups, —CH2— (methylene), —CH2CH2—, —CH2C(CH3)2CH2—, —CH2CH2CH2—, and




are non-limiting examples of alkanediyl groups. The term “alkylidene” when used without the “substituted” modifier refers to the divalent group ═CRR′ in which R and R′ are independently hydrogen, alkyl, or R and R′ are taken together to represent an alkanediyl having at least two carbon atoms. Non-limiting examples of alkylidene groups include: ═CH2, ═CH(CH2CH3), and ═C(CH3)2. When the term is used with the “substituted” modifier one or more hydrogen atom has been independently replaced by one of the following exemplary non-limiting functional groups: —OH, —F, —Cl, —Br, —I, —NH2, —NO2, —CO2H, —CO2CH3, —CN, —SH, —OCH3, —OCH2CH3, —C(O)CH3, —N(CH3)2, —C(O)NH2, —B(OH)2, —P(O)(OCH3)2 or —OC(O)CH3. The following groups are non-limiting examples of substituted alkyl groups: —CH2OH, —CH2Cl, —CF3, —CH2CN, —CH2C(O)OH, —CH2C(O)OCH3, —CH2C(O)NH2, —CH2C(O)CH3, —CH2OCH3, —CH2OC(O)CH3, —CH2NH2, —CH2N(CH3)2, and —CH2CH2Cl. The term “fluoroalkyl” is a subset of substituted alkyl, in which one or more hydrogen has been substituted with a fluoro group and no other atoms aside from carbon, hydrogen and fluorine are present. The groups, —CH2F, —CF3, and —CH2CF3 are non-limiting examples of fluoroalkyl groups. An “alkane” refers to the compound H—R, wherein R is alkyl.

The term “aryl” when used without the “substituted” modifier refers to a monovalent unsaturated aromatic group with an aromatic carbon atom as the point of attachment, said carbon atom forming part of a one or more six-membered aromatic ring structure, wherein the ring atoms are all carbon, and wherein the group consists of no atoms other than carbon and hydrogen. If more than one ring is present, the rings may be fused or unfused. As used herein, the term does not preclude the presence of one or more alkyl group (carbon number limitation permitting) attached to the first aromatic ring or any additional aromatic ring present. Non-limiting examples of aryl groups include phenyl (Ph), methylphenyl, (dimethyl)phenyl, —C6H4—CH2CH3 (ethylphenyl), naphthyl, and the monovalent group derived from biphenyl. The term “arenediyl” when used without the “substituted” modifier refers to a divalent aromatic group, with two aromatic carbon atoms as points of attachment, said carbon atoms forming part of one or more six-membered aromatic ring structure(s) wherein the ring atoms are all carbon, and wherein the monovalent group consists of no atoms other than carbon and hydrogen. As used herein, the term does not preclude the presence of one or more alkyl group (carbon number limitation permitting) attached to the first aromatic ring or any additional aromatic ring present. If more than one ring is present, the rings may be fused or unfused. Non-limiting examples of arenediyl groups include:




When the term “aryl” is used with the “substituted” modifier one or more hydrogen atom has been independently replaced by one of the following exemplary non-limiting functional groups: —OH, —F, —Cl, —Br, —I, —NH2, —NO2, —CO2H, —CO2CH3, —CN, —SH, —OCH3, —OCH2CH3, —C(O)CH3, —N(CH3)2, —C(O)NH2, —B(OH)2, —P(O)(OCH3)2 or —OC(O)CH3. An “arene” refers to the compound H—R, wherein R is aryl.
The term “aralkyl” when used without the “substituted” modifier refers to the monovalent group -alkanediyl-aryl, in which the terms alkanediyl and aryl are each used in a manner consistent with the definitions provided above. Non-limiting examples of aralkyls are: phenylmethyl (benzyl, Bn) and 2-phenyl-ethyl. When the term is used with the “substituted” modifier one or more hydrogen atom has been independently replaced by one of the following exemplary non-limiting functional groups: —OH, —F, —Cl, —Br, —I, —NH2, —NO2, —CO2H, —CO2CH3, —CN, —SH, —OCH3, —OCH2CH3, —C(O)CH3, —N(CH3)2, —C(O)NH2, —B(OH)2, —P(O)(OCH3)2 or —OC(O)CH3. Non-limiting examples of substituted aralkyls are: (3-chlorophenyl)-methyl, and 2-chloro-2-phenyl-eth-1-yl.
The term “heteroaryl” when used without the “substituted” modifier refers to a monovalent aromatic group with an aromatic carbon atom or nitrogen atom as the point of attachment, said carbon atom or nitrogen atom forming part of an aromatic ring structure wherein at least one of the ring atoms is nitrogen, oxygen or sulfur, and wherein the group consists of no atoms other than carbon, hydrogen, aromatic nitrogen, aromatic oxygen and aromatic sulfur. As used herein, the term does not preclude the presence of one or more alkyl group (carbon number limitation permitting) attached to the aromatic ring or any additional aromatic ring present. Non-limiting examples of heteroaryl groups include furanyl, imidazolyl, indolyl, indazolyl (Im), methylpyridyl, oxazolyl, pyridyl, pyrrolyl, pyrimidyl, pyrazinyl, quinolyl, quinazolyl, quinoxalinyl, thienyl, and triazinyl. The term “heteroarenediyl” when used without the “substituted” modifier refers to an divalent aromatic group, with two aromatic carbon atoms, two aromatic nitrogen atoms, or one aromatic carbon atom and one aromatic nitrogen atom as the two points of attachment, said atoms forming part of one or more aromatic ring structure(s) wherein at least one of the ring atoms is nitrogen, oxygen or sulfur, and wherein the divalent group consists of no atoms other than carbon, hydrogen, aromatic nitrogen, aromatic oxygen and aromatic sulfur. As used herein, the term does not preclude the presence of one or more alkyl group (carbon number limitation permitting) attached to the first aromatic ring or any additional aromatic ring present. If more than one ring is present, the rings may be fused or unfused. Non-limiting examples of heteroarenediyl groups include:




When the term is used with the “substituted” modifier one or more hydrogen atom has been independently replaced by one of the following exemplary non-limiting functional groups: —OH, —F, —Cl, —Br, —I, —NH2, —NO2, —CO2H, —CO2CH3, —CN, —SH, —OCH3, —OCH2CH3, —C(O)CH3, —N(CH3)2, —C(O)NH2, —B(OH)2, —P(O)(OCH3)2 or —OC(O)CH3.
The term “acyl” when used without the “substituted” modifier refers to the group —C(O)R, in which R is a hydrogen, alkyl, aryl, aralkyl or heteroaryl, as those terms are defined above. The groups, —CHO, —C(O)CH3 (acetyl, Ac), —C(O)CH2CH3, —C(O)CH2CH2CH3, —C(O)CH(CH3)2, —C(O)CH(CH2)2, —C(O)C6H5, —C(O)C6H4—CH3, —C(O)CH2C6H5, —C(O)(imidazolyl) are non-limiting examples of acyl groups. A “thioacyl” is defined in an analogous manner, except that the oxygen atom of the group —C(O)R has been replaced with a sulfur atom, —C(S)R. When the term is used with the “substituted” modifier one or more hydrogen atom has been independently replaced by one of the following exemplary non-limiting functional groups: —OH, —F, —Cl, —Br, —I, —NH2, —NO2, —CO2H, —CO2CH3, —CN, —SH, —OCH3, —OCH2CH3, —C(O)CH3, —N(CH3)2, —C(O)NH2, —B(OH)2, —P(O)(OCH3)2 or —OC(O)CH3. The groups, —C(O)CH2CF3, —CO2H (carboxyl), —CO2CH3 (methylcarboxyl), —CO2CH2CH3, —C(O)NH2 (carbamoyl), and —CON(CH3)2, are non-limiting examples of substituted acyl groups.
The term “alkoxy” when used without the “substituted” modifier refers to the group —OR, in which R is an alkyl, as that term is defined above. Non-limiting examples of alkoxy groups include: —OCH3, —OCH2CH3, —OCH2CH2CH3, —OCH(CH3)2, —OCH(CH2)2, —O-cyclopentyl, and —O-cyclohexyl. The terms “alkenyloxy”, “alkynyloxy”, “aryloxy”, “aralkoxy”, “heteroaryloxy”, and “acyloxy”, when used without the “substituted” modifier, refers to groups, defined as —OR, in which R is alkenyl, alkynyl, aryl, aralkyl, heteroaryl, and acyl, respectively. Similarly, the term “alkylthio” when used without the “substituted” modifier refers to the group —SR, in which R is an alkyl, as that term is defined above. When the term is used with the “substituted” modifier one or more hydrogen atom has been independently replaced by one of the following exemplary non-limiting functional groups: —OH, —F, —Cl, —Br, —I, —NH2, —NO2, —CO2H, —CO2CH3, —CN, —SH, —OCH3, —OCH2CH3, —C(O)CH3, —N(CH3)2, —C(O)NH2, —B(OH)2, —P(O)(OCH3)2 or —OC(O)CH3. The term “alcohol” corresponds to an alkane, as defined above, wherein at least one of the hydrogen atoms has been replaced with a hydroxy group.
The term “alkylamino” when used without the “substituted” modifier refers to the group —NHR, in which R is an alkyl, as that term is defined above. Non-limiting examples of alkylamino groups include: —NHCH3 and —NHCH2CH3. The term “dialkylamino” when used without the “substituted” modifier refers to the group —NRR′, in which R and R′ can be the same or different alkyl groups, or R and R′ can be taken together to represent an alkanediyl. Non-limiting examples of dialkylamino groups include: —N(CH3)2, —N(CH3)(CH2CH3), and N-pyrrolidinyl. The terms “alkoxyamino”, “alkenylamino”, “alkynylamino”, “arylamino”, “aralkylamino”, “heteroarylamino”, and “alkylsulfonylamino” when used without the “substituted” modifier, refers to groups, defined as —NHR, in which R is alkoxy, alkenyl, alkynyl, aryl, aralkyl, heteroaryl, and alkylsulfonyl, respectively. A non-limiting example of an arylamino group is —NHC6H5. The term “amido” (acylamino), when used without the “substituted” modifier, refers to the group —NHR, in which R is acyl, as that term is defined above. A non-limiting example of an amido group is —NHC(O)CH3. The term “alkylimino” when used without the “substituted” modifier refers to the divalent group ═NR, in which R is an alkyl, as that term is defined above. When the term is used with the “substituted” modifier one or more hydrogen atom has been independently replaced by one of the following exemplary non-limiting functional groups: —OH, —F, —Cl, —Br, —I, —NH2, —NO2, —CO2H, —CO2CH3, —CN, —SH, —OCH3, —OCH2CH3, —C(O)CH3, —N(CH3)2, —C(O)NH2, —B(OH)2, —P(O)(OCH3)2 or —OC(O)CH3. The groups —NHC(O)OCH3 and —NHC(O)NHCH3 are non-limiting examples of substituted amido groups.
The term “heterocyclic” or “heterocycle” when used without the “substituted” modifier signifies that the compound/group so modified comprising at least one ring in which at least one ring atom is an element other than carbon. Examples of the non-carbon ring atoms include but are not limited to nitrogen, oxygen, sulfur, boron, phosphorus, arsenic, antimony, germanium, bismuth, silicon and/or tin. Examples of heterocyclic structures include but are not limited to aziridine, azirine, oxirane, epoxide, oxirene, thiirane, episulfides, thiirene, diazirine, oxaziridine, dioxirane, azetidine, azete, oxetane, oxete, thietane, thiete, diazetidine, dioxetane, dioxete, dithietane, dithiete, pyrrolidine, pyrrole, oxolane, furane, thiolane, thiophene, borolane, borole, phospholane, phosphole, arsolane, arsole, stibolane, stibole, bismolane, bismole, silolane, silole, stannolane, stannole, imidazolidine, imidazole, pyrazolidine, pyrazole, imidazoline, pyrazoline, oxazolidine, oxazole, oxazoline, isoxazolidine, isoxazole, thiazolidine, thiazole, thiazoline, isothiazolidine, isothiazole, dioxolane, thithiolane, triazole, furazan, oxadiazole, thiadiazole, dithiazole, tetrazole, piperidine, pyridine, oxane, pyran, thiane, thiopyran, salinane, saline, germinane, germine, stanninane, stannine, borinane, borinine, phosphinane, phosphinine, arsinane, arsinine, piperazine, diazine, morpholine, oxazine, thiomorpholine, thiazine, dioxane, dioxine, dithiane, dithiine, triazine, trioxane, tetrazine, azepane, azepine, oxepane, oxepine, thiepane, thiepine, homopiperazine, diazepine, thiazepine, ozocane, azocine, oxecane, or thiocane. When the term “heterocyclic” is used with the “substituted” modifier one or more hydrogen atom has been independently replaced by one of the following exemplary non-limiting functional groups: —OH, —F, —Cl, —Br, —I, —NH2, —NO2, —CO2H, —CO2CH3, —CN, —SH, —OCH3, —OCH2CH3, —C(O)CH3, —N(CH3)2, —C(O)NH2 or —OC(O)CH3.
As generally used herein “pharmaceutically acceptable” refers to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues, organs, and/or bodily fluids of human beings and animals without excessive toxicity, irritation, allergic response, or other problems or complications commensurate with a reasonable benefit/risk ratio.
“Pharmaceutically acceptable salts” means salts of compounds of the present invention which are pharmaceutically acceptable, as defined above, and which possess the desired pharmacological activity. Such salts include acid addition salts formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like; or with organic acids such as 1,2-ethanedisulfonic acid, 2-hydroxyethanesulfonic acid, 2-naphthalenesulfonic acid, 3-phenylpropionic acid, 4,4′-methylenebis(3-hydroxy-2-ene-1-carboxylic acid), 4-methylbicyclo[2.2.2]oct-2-ene-1-carboxylic acid, acetic acid, aliphatic mono- and dicarboxylic acids, aliphatic sulfuric acids, aromatic sulfuric acids, benzenesulfonic acid, benzoic acid, camphorsulfonic acid, carbonic acid, cinnamic acid, citric acid, cyclopentanepropionic acid, ethanesulfonic acid, fumaric acid, glucoheptonic acid, gluconic acid, glutamic acid, glycolic acid, heptanoic acid, hexanoic acid, hydroxynaphthoic acid, lactic acid, laurylsulfuric acid, maleic acid, malic acid, malonic acid, mandelic acid, methanesulfonic acid, muconic acid, o-(4-hydroxybenzoyl)benzoic acid, oxalic acid, p-chlorobenzenesulfonic acid, phenyl-substituted alkanoic acids, propionic acid, p-toluenesulfonic acid, pyruvic acid, salicylic acid, stearic acid, succinic acid, tartaric acid, tertiarybutylacetic acid, trimethylacetic acid, trifluoroacetic acid, trifluormethyl sulfonic (triflic) acid and the like. Pharmaceutically acceptable salts also include base addition salts which may be formed when acidic protons present are capable of reacting with inorganic or organic bases. Acceptable inorganic bases include sodium hydroxide, sodium carbonate, potassium hydroxide, aluminum hydroxide and calcium hydroxide. Acceptable organic bases include, but are not limited to ethanolamine, diethanolamine, triethanolamine, tromethamine, N-methylglucamine and the like. It should be recognized that the particular anion or cation forming a part of any salt of this invention is not critical, so long as the salt, as a whole, is pharmacologically acceptable. Additional examples of pharmaceutically acceptable salts and their methods of preparation and use are presented in Handbook of Pharmaceutical Salts: Properties, and Use (P. H. Stahl & C. G. Wermuth eds., Verlag Helvetica Chimica Acta, 2002).
VIII. Pharmaceutical Preparations
Pharmaceutical compositions of the present invention comprise an effective amount of one or more didemnin or didemnin derivative compositions of the invention dissolved or dispersed in a pharmaceutically acceptable carrier. The phrases “pharmaceutical or pharmacologically acceptable” refers to molecular entities and compositions that do not produce an adverse, allergic or other untoward reaction when administered to an animal, such as, for example, a human, as appropriate. The preparation of an pharmaceutical composition that contains at least one composition of the invention or additional active ingredient will be known to those of skill in the art in light of the present disclosure, as exemplified by Remington's Pharmaceutical Sciences, 18th Ed. Mack Printing Company, 1990, incorporated herein by reference. Moreover, for animal (e.g., human) administration, it will be understood that preparations should meet sterility, pyrogenicity, general safety and purity standards as required by FDA Office of Biological Standards.
As used herein, “pharmaceutically acceptable carrier” includes any and all solvents, dispersion media, coatings, surfactants, antioxidants, preservatives (e.g., antibacterial agents, antifungal agents), isotonic agents, absorption delaying agents, salts, preservatives, drugs, drug stabilizers, gels, binders, excipients, disintegration agents, lubricants, sweetening agents, flavoring agents, dyes, such like materials and combinations thereof, as would be known to one of ordinary skill in the art (see, for example, Remington's Pharmaceutical Sciences, 18th Ed. Mack Printing Company, 1990, pp. 1289-1329, incorporated herein by reference). Except insofar as any conventional carrier is incompatible with the active ingredient, its use in the pharmaceutical compositions is contemplated.
The didemnin or didemnin derivative may comprise different types of carriers depending on whether it is to be administered in solid, liquid or aerosol form, and whether it need to be sterile for such routes of administration as injection. The present invention can be administered intravenously, intradermally, transdermally, intrathecally, intraarterially, intraperitoneally, intranasally, intravaginally, intrarectally, topically, intramuscularly, subcutaneously, mucosally, orally, topically, locally, inhalation (e.g., aerosol inhalation), injection, infusion, continuous infusion, localized perfusion bathing target cells directly, via a catheter, via a lavage, in cremes, in lipid compositions (e.g., liposomes), or by other method or any combination of the forgoing as would be known to one of ordinary skill in the art (see, for example, Remington's Pharmaceutical Sciences, 18th Ed. Mack Printing Company, 1990, incorporated herein by reference).
The didemnin or didemnin derivative may be formulated into a composition in a free base, neutral or salt form. Pharmaceutically acceptable salts, include the acid addition salts, e.g., those formed with the free amino groups of a proteinaceous composition, or which are formed with inorganic acids such as for example, hydrochloric or phosphoric acids, or such organic acids as acetic, oxalic, tartaric or mandelic acid. Salts formed with the free carboxyl groups can also be derived from inorganic bases such as for example, sodium, potassium, ammonium, calcium or ferric hydroxides; or such organic bases as isopropylamine, trimethylamine, histidine or procaine. Upon formulation, solutions will be administered in a manner compatible with the dosage formulation and in such amount as is therapeutically effective. The formulations are easily administered in a variety of dosage forms such as formulated for parenteral administrations such as injectable solutions, or aerosols for delivery to the lungs, or formulated for alimentary administrations such as drug release capsules and the like.
Further in accordance with the present invention, the composition of the present invention suitable for administration is provided in a pharmaceutically acceptable carrier with or without an inert diluent. The carrier should be assimilable and includes liquid, semi-solid, i.e., pastes, or solid carriers. Except insofar as any conventional media, agent, diluent or carrier is detrimental to the recipient or to the therapeutic effectiveness of a the composition contained therein, its use in administrable composition for use in practicing the methods of the present invention is appropriate. Examples of carriers or diluents include fats, oils, water, saline solutions, lipids, liposomes, resins, binders, fillers and the like, or combinations thereof. The composition may also comprise various antioxidants to retard oxidation of one or more component. Additionally, the prevention of the action of microorganisms can be brought about by preservatives such as various antibacterial and antifungal agents, including but not limited to parabens (e.g., methylparabens, propylparabens), chlorobutanol, phenol, sorbic acid, thimerosal or combinations thereof.
In accordance with the present invention, the composition is combined with the carrier in any convenient and practical manner, i.e., by solution, suspension, emulsification, admixture, encapsulation, absorption and the like. Such procedures are routine for those skilled in the art.
In a specific embodiment of the present invention, the composition is combined or mixed thoroughly with a semi-solid or solid carrier. The mixing can be carried out in any convenient manner such as grinding. Stabilizing agents can be also added in the mixing process in order to protect the composition from loss of therapeutic activity, i.e., denaturation in the stomach. Examples of stabilizers for use in an the composition include buffers, amino acids such as glycine and lysine, carbohydrates such as dextrose, mannose, galactose, fructose, lactose, sucrose, maltose, sorbitol, mannitol, etc.
In further embodiments, the present invention may concern the use of a pharmaceutical lipid vehicle compositions that include a didemnin or didemnin derivative, one or more lipids, and an aqueous solvent. As used herein, the term “lipid” will be defined to include any of a broad range of substances that is characteristically insoluble in water and extractable with an organic solvent. This broad class of compounds are well known to those of skill in the art, and as the term “lipid” is used herein, it is not limited to any particular structure. Examples include compounds which contain long-chain aliphatic hydrocarbons and their derivatives. A lipid may be naturally occurring or synthetic (i.e., designed or produced by man). However, a lipid is usually a biological substance. Biological lipids are well known in the art, and include for example, neutral fats, phospholipids, phosphoglycerides, steroids, terpenes, lysolipids, glycosphingolipids, glycolipids, sulphatides, lipids with ether and ester-linked fatty acids and polymerizable lipids, and combinations thereof. Of course, compounds other than those specifically described herein that are understood by one of skill in the art as lipids are also encompassed by the compositions and methods of the present invention.
One of ordinary skill in the art would be familiar with the range of techniques that can be employed for dispersing a composition in a lipid vehicle. For example, the didemnin or didemnin derivative may be dispersed in a solution containing a lipid, dissolved with a lipid, emulsified with a lipid, mixed with a lipid, combined with a lipid, covalently bonded to a lipid, contained as a suspension in a lipid, contained or complexed with a micelle or liposome, or otherwise associated with a lipid or lipid structure by any means known to those of ordinary skill in the art. The dispersion may or may not result in the formation of liposomes.
The actual dosage amount of a composition of the present invention administered to an animal patient can be determined by physical and physiological factors such as body weight, severity of condition, the type of disease being treated, previous or concurrent therapeutic interventions, idiopathy of the patient and on the route of administration. Depending upon the dosage and the route of administration, the number of administrations of a preferred dosage and/or an effective amount may vary according to the response of the subject. The practitioner responsible for administration will, in any event, determine the concentration of active ingredient(s) in a composition and appropriate dose(s) for the individual subject.
In certain embodiments, pharmaceutical compositions may comprise, for example, at least about 0.1% of an active compound. In other embodiments, the an active compound may comprise between about 2% to about 75% of the weight of the unit, or between about 25% to about 60%, for example, and any range derivable therein. Naturally, the amount of active compound(s) in each therapeutically useful composition may be prepared is such a way that a suitable dosage will be obtained in any given unit dose of the compound. Factors such as solubility, bioavailability, biological half-life, route of administration, product shelf life, as well as other pharmacological considerations will be contemplated by one skilled in the art of preparing such pharmaceutical formulations, and as such, a variety of dosages and treatment regimens may be desirable.
In other non-limiting examples, a dose may also comprise from about 1 microgram/kg/body weight, about 5 microgram/kg/body weight, about 10 microgram/kg/body weight, about 50 microgram/kg/body weight, about 100 microgram/kg/body weight, about 200 microgram/kg/body weight, about 350 microgram/kg/body weight, about 500 microgram/kg/body weight, about 1 milligram/kg/body weight, about 5 milligram/kg/body weight, about 10 milligram/kg/body weight, about 50 milligram/kg/body weight, about 100 milligram/kg/body weight, about 200 milligram/kg/body weight, about 350 milligram/kg/body weight, about 500 milligram/kg/body weight, to about 1000 mg/kg/body weight or more per administration, and any range derivable therein. In non-limiting examples of a derivable range from the numbers listed herein, a range of about 5 mg/kg/body weight to about 100 mg/kg/body weight, about 5 microgram/kg/body weight to about 500 milligram/kg/body weight, etc., can be administered, based on the numbers described above.
D. Alimentary Compositions and Formulations
In preferred embodiments of the present invention, the composition(s) are formulated to be administered via an alimentary route. Alimentary routes include all possible routes of administration in which the composition is in direct contact with the alimentary tract. Specifically, the pharmaceutical compositions disclosed herein may be administered orally, buccally, rectally, or sublingually. As such, these compositions may be formulated with an inert diluent or with an assimilable edible carrier, or they may be enclosed in hard- or soft-shell gelatin capsule, or they may be compressed into tablets, or they may be incorporated directly with the food of the diet.
In certain embodiments, the active compounds may be incorporated with excipients and used in the form of ingestible tablets, buccal tables, troches, capsules, elixirs, suspensions, syrups, wafers, and the like (Mathiowitz et al., 1997; Hwang et al., 1998; U.S. Pat. Nos. 5,641,515; 5,580,579 and 5,792,451, each specifically incorporated herein by reference in its entirety). The tablets, troches, pills, capsules and the like may also contain the following: a binder, such as, for example, gum tragacanth, acacia, cornstarch, gelatin or combinations thereof; an excipient, such as, for example, dicalcium phosphate, mannitol, lactose, starch, magnesium stearate, sodium saccharine, cellulose, magnesium carbonate or combinations thereof; a disintegrating agent, such as, for example, corn starch, potato starch, alginic acid or combinations thereof; a lubricant, such as, for example, magnesium stearate; a sweetening agent, such as, for example, sucrose, lactose, saccharin or combinations thereof; a flavoring agent, such as, for example peppermint, oil of wintergreen, cherry flavoring, orange flavoring, etc. When the dosage unit form is a capsule, it may contain, in addition to materials of the above type, a liquid carrier. Various other materials may be present as coatings or to otherwise modify the physical form of the dosage unit. For instance, tablets, pills, or capsules may be coated with shellac, sugar, or both. When the dosage form is a capsule, it may contain, in addition to materials of the above type, carriers such as a liquid carrier. Gelatin capsules, tablets, or pills may be enterically coated. Enteric coatings prevent denaturation of the composition in the stomach or upper bowel where the pH is acidic. See, e.g., U.S. Pat. No. 5,629,001. Upon reaching the small intestines, the basic pH therein dissolves the coating and permits the composition to be released and absorbed by specialized cells, e.g., epithelial enterocytes and Peyer's patch M cells. A syrup of elixir may contain the active compound sucrose as a sweetening agent methyl and propylparabens as preservatives, a dye and flavoring, such as cherry or orange flavor. Of course, any material used in preparing any dosage unit form should be pharmaceutically pure and substantially non-toxic in the amounts employed. In addition, the active compounds may be incorporated into sustained-release preparation and formulations.
For oral administration the compositions of the present invention may alternatively be incorporated with one or more excipients in the form of a mouthwash, dentifrice, buccal tablet, oral spray, or sublingual orally-administered formulation. For example, a mouthwash may be prepared incorporating the active ingredient in the required amount in an appropriate solvent, such as a sodium borate solution (Dobell's Solution). Alternatively, the active ingredient may be incorporated into an oral solution such as one containing sodium borate, glycerin and potassium bicarbonate, or dispersed in a dentifrice, or added in a therapeutically-effective amount to a composition that may include water, binders, abrasives, flavoring agents, foaming agents, and humectants. Alternatively the compositions may be fashioned into a tablet or solution form that may be placed under the tongue or otherwise dissolved in the mouth.
Additional formulations which are suitable for other modes of alimentary administration include suppositories. Suppositories are solid dosage forms of various weights and shapes, usually medicated, for insertion into the rectum. After insertion, suppositories soften, melt or dissolve in the cavity fluids. In general, for suppositories, traditional carriers may include, for example, polyalkylene glycols, triglycerides or combinations thereof. In certain embodiments, suppositories may be formed from mixtures containing, for example, the active ingredient in the range of about 0.5% to about 10%, and preferably about 1% to about 2%.
E. Parenteral Compositions and Formulations
In further embodiments, the composition may be administered via a parenteral route. As used herein, the term “parenteral” includes routes that bypass the alimentary tract. Specifically, the pharmaceutical compositions disclosed herein may be administered for example, but not limited to intravenously, intradermally, intramuscularly, intraarterially, intrathecally, subcutaneous, or intraperitoneally U.S. Pat. Nos. 6,7537,514, 6,613,308, 5,466,468, 5,543,158; 5,641,515; and 5,399,363 (each specifically incorporated herein by reference in its entirety).
Solutions of the active compounds as free base or pharmacologically acceptable salts may be prepared in water suitably mixed with a surfactant, such as hydroxypropylcellulose. Dispersions may also be prepared in glycerol, liquid polyethylene glycols, and mixtures thereof and in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of microorganisms. The pharmaceutical forms suitable for injectable use include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions (U.S. Pat. No. 5,466,468, specifically incorporated herein by reference in its entirety). In all cases the form must be sterile and must be fluid to the extent that easy injectability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms, such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (i.e., glycerol, propylene glycol, and liquid polyethylene glycol, and the like), suitable mixtures thereof, and/or vegetable oils. Proper fluidity may be maintained, for example, by the use of a coating, such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. The prevention of the action of microorganisms can be brought about by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic agents, for example, sugars or sodium chloride. Prolonged absorption of the injectable compositions can be brought about by the use in the compositions of agents delaying absorption, for example, aluminum monostearate and gelatin.
For parenteral administration in an aqueous solution, for example, the solution should be suitably buffered if necessary and the liquid diluent first rendered isotonic with sufficient saline or glucose. These particular aqueous solutions are especially suitable for intravenous, intramuscular, subcutaneous, and intraperitoneal administration. In this connection, sterile aqueous media that can be employed will be known to those of skill in the art in light of the present disclosure. For example, one dosage may be dissolved in isotonic NaCl solution and either added hypodermoclysis fluid or injected at the proposed site of infusion, (see for example, “Remington's Pharmaceutical Sciences” 15th Edition, pages 1035-1038 and 1570-1580). Some variation in dosage will necessarily occur depending on the condition of the subject being treated. The person responsible for administration will, in any event, determine the appropriate dose for the individual subject. Moreover, for human administration, preparations should meet sterility, pyrogenicity, general safety and purity standards as required by FDA Office of Biologics standards.
Sterile injectable solutions are prepared by incorporating the active compounds in the required amount in the appropriate solvent with various of the other ingredients enumerated above, as required, followed by filtered sterilization. Generally, dispersions are prepared by incorporating the various sterilized active ingredients into a sterile vehicle which contains the basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum-drying and freeze-drying techniques which yield a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof. A powdered composition is combined with a liquid carrier such as, e.g., water or a saline solution, with or without a stabilizing agent.
F. Miscellaneous Pharmaceutical Compositions and Formulations
In other preferred embodiments of the invention, the active compound may be formulated for administration via various miscellaneous routes, for example, topical (i.e., transdermal) administration, mucosal administration (intranasal, vaginal, etc.) and/or inhalation.
Pharmaceutical compositions for topical administration may include the active compound formulated for a medicated application such as an ointment, paste, cream or powder. Ointments include all oleaginous, adsorption, emulsion and water-solubly based compositions for topical application, while creams and lotions are those compositions that include an emulsion base only. Topically administered medications may contain a penetration enhancer to facilitate adsorption of the active ingredients through the skin. Suitable penetration enhancers include glycerin, alcohols, alkyl methyl sulfoxides, pyrrolidones and luarocapram. Possible bases for compositions for topical application include polyethylene glycol, lanolin, cold cream and petrolatum as well as any other suitable absorption, emulsion or water-soluble ointment base. Topical preparations may also include emulsifiers, gelling agents, and antimicrobial preservatives as necessary to preserve the active ingredient and provide for a homogenous mixture. Transdermal administration of the present invention may also comprise the use of a “patch”. For example, the patch may supply one or more active substances at a predetermined rate and in a continuous manner over a fixed period of time.
In certain embodiments, the pharmaceutical compositions may be delivered by eye drops, intranasal sprays, inhalation, and/or other aerosol delivery vehicles. Methods for delivering compositions directly to the lungs via nasal aerosol sprays has been described e.g., in U.S. Pat. Nos. 5,756,353 and 5,804,212 (each specifically incorporated herein by reference in its entirety). Likewise, the delivery of drugs using intranasal microparticle resins (Takenaga et al., 1998) and lysophosphatidyl-glycerol compounds (U.S. Pat. No. 5,725,871, specifically incorporated herein by reference in its entirety) are also well-known in the pharmaceutical arts. Likewise, transmucosal drug delivery in the form of a polytetrafluoroetheylene support matrix is described in U.S. Pat. No. 5,780,045 (specifically incorporated herein by reference in its entirety).
The term aerosol refers to a colloidal system of finely divided solid of liquid particles dispersed in a liquefied or pressurized gas propellant. The typical aerosol of the present invention for inhalation will consist of a suspension of active ingredients in liquid propellant or a mixture of liquid propellant and a suitable solvent. Suitable propellants include hydrocarbons and hydrocarbon ethers. Suitable containers will vary according to the pressure requirements of the propellant. Administration of the aerosol will vary according to subject's age, weight and the severity and response of the symptoms.
IX. Combination Therapy
In some embodiments, in order to increase the effectiveness of a didemnin or didemnin derivative, it may be desirable to combine these compositions with other agents effective in the treatment of hyperproliferative disease, such as anti-cancer agents. An “anti-cancer” agent is capable of negatively affecting cancer in a subject, for example, by killing cancer cells, inducing apoptosis in cancer cells, reducing the growth rate of cancer cells, reducing the incidence or number of metastases, reducing tumor size, inhibiting tumor growth, reducing the blood supply to a tumor or cancer cells, promoting an immune response against cancer cells or a tumor, preventing or inhibiting the progression of cancer, or increasing the lifespan of a subject with cancer. More generally, these other compositions would be provided in a combined amount effective to kill or inhibit proliferation of the cell. This process may involve contacting the cells with the didemnin or didemnin derivative and the agent(s) or multiple factor(s) at the same time. This may be achieved by contacting the cell with a single composition or pharmacological formulation that includes both agents, or by contacting the cell with two distinct compositions or formulations, at the same time, wherein one composition includes the expression construct and the other includes the second agent(s).
Tumor cell resistance to chemotherapy and radiotherapy agents represents a major problem in clinical oncology. One goal of current cancer research is to find ways to improve the efficacy of chemo- and radiotherapy by combining it with other therapy. In the context of the present invention, it is contemplated that a didemnin or didemnin derivative could be used in conjunction with chemotherapeutic, radiotherapeutic, or immunotherapeutic intervention, for example.
The didemnin or didemnin derivative therapy may precede or follow the other agent treatment by intervals ranging from minutes to weeks. In embodiments where the other agent and expression construct are applied separately to the cell, one would generally ensure that a significant period of time did not expire between the time of each delivery, such that the agent and didemnin or didemnin derivative would still be able to exert an advantageously combined effect on the cell. In such instances, it is contemplated that one may contact the cell with both modalities within about 12-24 h of each other and, more preferably, within about 6-12 h of each other. In some situations, it may be desirable to extend the time period for treatment significantly, however, where several d (2, 3, 4, 5, 6 or 7) to several wk (1, 2, 3, 4, 5, 6, 7 or 8) lapse between the respective administrations.
Various combinations may be employed, didemnin or didemnin derivative therapy is “A” and the secondary agent, such as radio- or chemotherapy (for example), is “B”:
A/B/A B/A/B B/B/A A/A/B A/B/B B/A/A A/B/B/B B/A/B/B
B/B/B/A B/B/A/B A/A/B/B A/B/A/B A/B/B/A B/B/A/A
B/A/B/A B/A/A/B A/A/A/B B/A/A/A A/B/A/A A/A/B/A
Administration of the therapeutic didemnin or didemnin derivative of the present invention to a patient will follow general protocols for the administration of chemotherapeutics, taking into account the toxicity, if any, of the vector. It is expected that the treatment cycles would be repeated as necessary. It also is contemplated that various standard therapies, as well as surgical intervention, may be applied in combination with the described hyperproliferative cell therapy.
A. Chemotherapy
Cancer therapies also include a variety of combination therapies with both chemical and radiation based treatments. Combination chemotherapies include, for example, cisplatin (CDDP), carboplatin, procarbazine, mechlorethamine, cyclophosphamide, camptothecin, ifosfamide, melphalan, chlorambucil, busulfan, nitro surea, dactinomycin, daunorubicin, doxorubicin, bleomycin, plicomycin, mitomycin, etoposide (VP16), tamoxifen, raloxifene, estrogen receptor binding agents, taxol, gemcitabien, navelbine, farnesyl-protein tansferase inhibitors, transplatinum, 5-fluorouracil, vincristin, vinblastin and methotrexate, or any analog or derivative variant of the foregoing.
B. Radiotherapy
Other factors that cause DNA damage and have been used extensively include what are commonly known as 7-rays, X-rays, and/or the directed delivery of radioisotopes to tumor cells. Other forms of DNA damaging factors are also contemplated such as microwaves and UV-irradiation. It is most likely that all of these factors effect a broad range of damage on DNA, on the precursors of DNA, on the replication and repair of DNA, and on the assembly and maintenance of chromosomes. Dosage ranges for X-rays range from daily doses of 50 to 200 roentgens for prolonged periods of time (3 to 4 wk), to single doses of 2000 to 6000 roentgens. Dosage ranges for radioisotopes vary widely, and depend on the half-life of the isotope, the strength and type of radiation emitted, and the uptake by the neoplastic cells.
The terms “contacted” and “exposed,” when applied to a cell, are used herein to describe the process by which a therapeutic construct and a chemotherapeutic or radiotherapeutic agent are delivered to a target cell or are placed in direct juxtaposition with the target cell. To achieve cell killing or stasis, both agents are delivered to a cell in a combined amount effective to kill the cell or prevent it from dividing.
C. Immunotherapy
Immunotherapeutics, generally, rely on the use of immune effector cells and molecules to target and destroy cancer cells. The immune effector may be, for example, an antibody specific for some marker on the surface of a tumor cell. The antibody alone may serve as an effector of therapy or it may recruit other cells to actually effect cell killing. The antibody also may be conjugated to a drug or toxin (chemotherapeutic, radionuclide, ricin A chain, cholera toxin, pertussis toxin, etc.) and serve merely as a targeting agent. Alternatively, the effector may be a lymphocyte carrying a surface molecule that interacts, either directly or indirectly, with a tumor cell target. Various effector cells include cytotoxic T cells and NK cells.
Immunotherapy, thus, could be used as part of a combined therapy, in conjunction with didemnin or didemnin derivative therapy. The general approach for combined therapy is discussed below. Generally, the tumor cell must bear some marker that is amenable to targeting, i.e., is not present on the majority of other cells. Many tumor markers exist and any of these may be suitable for targeting in the context of the present invention. Common tumor markers include carcinoembryonic antigen, prostate specific antigen, urinary tumor associated antigen, fetal antigen, tyrosinase (p97), gp68, TAG-72, HMFG, Sialyl Lewis Antigen, MucA, MucB, PLAP, estrogen receptor, laminin receptor, erb B and p155.
D. Genes
In yet another embodiment, the secondary treatment is a gene therapy in which a therapeutic polynucleotide is administered before, after, or at the same time as the didemnin or didemnin derivative. Delivery of didemnin or a didemnin derivative with a vector encoding one of a therapeutic gene product will have a combined anti-hyperproliferative effect on target tissues. Alternatively, a single vector encoding both genes may be used. A variety of proteins are encompassed within the invention, including inducers of cellular proliferation, inhibitors of cellular proliferation, or regulators of programmed cell death, for example.
E. Surgery
Approximately 60% of persons with cancer will undergo surgery of some type, which includes preventative, diagnostic or staging, curative and palliative surgery. Curative surgery is a cancer treatment that may be used in conjunction with other therapies, such as the treatment of the present invention, chemotherapy, radiotherapy, hormonal therapy, gene therapy, immunotherapy and/or alternative therapies.
Curative surgery includes resection in which all or part of cancerous tissue is physically removed, excised, and/or destroyed. Tumor resection refers to physical removal of at least part of a tumor. In addition to tumor resection, treatment by surgery includes laser surgery, cryosurgery, electrosurgery, and miscopically controlled surgery (Mohs' surgery). It is further contemplated that the present invention may be used in conjunction with removal of superficial cancers, precancers, or incidental amounts of normal tissue.
Upon excision of part of all of cancerous cells, tissue, or tumor, a cavity may be formed in the body. Treatment may be accomplished by perfusion, direct injection or local application of the area with an additional anti-cancer therapy. Such treatment may be repeated, for example, every 1, 2, 3, 4, 5, 6, or 7 days, or every 1, 2, 3, 4, and 5 weeks or every 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 months. These treatments may be of varying dosages as well.
F. Other Agents
It is contemplated that other agents may be used in combination with the present invention to improve the therapeutic efficacy of treatment. These additional agents include immunomodulatory agents, agents that affect the upregulation of cell surface receptors and GAP junctions, cytostatic and differentiation agents, inhibitors of cell adhesion, or agents that increase the sensitivity of the hyperproliferative cells to apoptotic inducers. Immunomodulatory agents include tumor necrosis factor; interferon alpha, beta, and gamma; IL-2 and other cytokines; F42K and other cytokine analogs; or MIP-1, MIP-1beta, MCP-1, RANTES, and other chemokines. It is further contemplated that the upregulation of cell surface receptors or their ligands such as Fas/Fas ligand, DR4 or DR5/TRAIL would potentiate the apoptotic inducing abilities of the present invention by establishment of an autocrine or paracrine effect on hyperproliferative cells. Increases intercellular signaling by elevating the number of GAP junctions would increase the anti-hyperproliferative effects on the neighboring hyperproliferative cell population. In other embodiments, cytostatic or differentiation agents can be used in combination with the present invention to improve the anti-hyerproliferative efficacy of the treatments. Inhibitors of cell adehesion are contemplated to improve the efficacy of the present invention. Examples of cell adhesion inhibitors are focal adhesion kinase (FAKs) inhibitors and Lovastatin. It is further contemplated that other agents that increase the sensitivity of a hyperproliferative cell to apoptosis, such as the antibody c225, could be used in combination with the present invention to improve the treatment efficacy.
Hormonal therapy may also be used in conjunction with the present invention or in combination with any other cancer therapy previously described. The use of hormones may be employed in the treatment of certain cancers such as breast, prostate, ovarian, or cervical cancer to lower the level or block the effects of certain hormones such as testosterone or estrogen. This treatment is often used in combination with at least one other cancer therapy as a treatment option or to reduce the risk of metastases.
X. Kits of the Invention
Any of the compositions described herein may be comprised in a kit, including, for example, one or more bacteria, media reagents, and so forth. In some embodiments, one or more reagents to assist in fermentation of didemnin in bacteria is provided in a kit. In certain aspects, one or more compounds for use in preparing a didemnin derivative from an endogenously produced Tistrella didemnin is included in the kit.
The kits may comprise a suitably aliquoted composition of the present invention. The components of the kits may be packaged either in aqueous media or in lyophilized form, where appropriate. The container means of the kits will generally include at least one vial, test tube, flask, bottle, syringe or other container means, into which a component may be placed, and preferably, suitably aliquoted. Where there are more than one component in the kit, the kit also will generally contain a second, third or other additional container into which the additional components may be separately placed. However, various combinations of components may be comprised in a vial. The kits of the present invention also will typically include a means for containing the kit component(s) in close confinement for commercial sale. Such containers may include injection or blow molded plastic containers into which the desired vials are retained.
However, the components of the kit may be provided as dried powder(s). When reagents and/or components are provided as a dry powder, the powder can be reconstituted by the addition of a suitable solvent. It is envisioned that the solvent may also be provided in another container means.
EXAMPLES
The following examples are included to demonstrate preferred embodiments of the invention. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered by the inventor to function well in the practice of the invention, and thus can be considered to constitute preferred modes for its practice. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention.
Example 1
Exemplary Methods
Strain, Fermentation and Isolation of Didemnin Compounds
Tistrella mobilis was isolated from seawater collected from the Red Sea during a 2009 research cruise. Its crude extract showed remarkable cytotoxicity on HeLa cells in a bioactive compound screening of Red Sea bacteria. T. mobilis was grown on GYP medium [10 g of glucose/4 g of yeast extract/2 g of peptone/17 g of sea salts/1 liter of deionized water] using 50-liter stirred fermenters at 25° C. for 72 h. At the end of fermentation, ethyl acetate was added to the culture to extract the metabolites. The crude extract was then fractionated by reverse-phase C18 liquid chromatography and eluted with increasing amounts of methanol in water. The active fraction was further purified by semi-preparative reverse-phase HPLC with 63% acetonitrile in water at a flow rate of 3 milliliter per minute. Didemnin B and nordidemnin B were eluted at 25 and 22 minute respectively.
Genome Sequencing, Annotation, and Analysis
The nucleotide sequence of the T. mobilis genome was determined by using a massively parallel pyrosequencing technology (Roche 454 GS FLX). 112 contigs (>500 bp) with a total size of 6.4 Mb were assembled from 315,496 reads (average length of 334 bp) using Newbler software of the 454 suite package, providing an 18.4-fold coverage. In addition, 2,625,640 sequences with average length 115 bps of mate-pair produced by the Illumina sequencing system were mapped to the genome sequence to promote sequence quality and construct a scaffold. All the contig relationships within scaffolds were validated by PCR, and the relationship among scaffolds was determined by multiplex PCR. Gaps were filled by sequencing PCR products. The final sequence assembly was carried out using phred/phrap/consed package (see the World Wide website of the Genome Center at the University of Washington), and the low sequence quality region was resequenced. The final error rate of genome sequence was 0.28 per 100,000 bases.
Protein-coding sequences (CDS) were determined by combining the prediction results of Glimmer 3.02 and Z-Curve programs. Functional annotation of CDS were performed by searching the NCBI non-redundant protein database and KEGG protein database. tRNA genes were predicted with tRNAScan-SE (v1.23). Protein domain prediction and COG assignment were performed by RPS-BLAST using the NCBI CDD library.
In Silico Analysis of Didemnin Biosynthetic Gene Cluster and Other NRPS in T. mobilis Genome
The roles of the proteins, in embodiments of the invention, in the didemnin gene cluster were assigned using protein-protein BLAST and Pfam analysis. The NRPS A domain specificity was predicted using online program NRPSpredictor (Rausch 2005). The nucleotide sequences of the gene cluster can be deposited at GenBank®, for example.
Example 2
General Genome Feature of Tistrella mobilis and Associated NRPS Gene Clusters
Isolation of Didemnins from Tistrella mobilis 
The bacterium was noted for its astonishing cytotoxic activity during a screening program of bacteria isolated from the Red Sea for cytotoxic activity. Its ethyl acetate crude extract of culture broth could kill the HeLa cells at a concentration lower than 1 ng/ml. The bacterium was then fermented to 70 l and the ethyl acetate extract was subjected to reverse phase column chromatography using 15%, 30%, 45%, 60%, 70%, 90% and 100% methanol in water, respectively. Fractions eluted using 60% and 70% showed strong cytotoxic activity using HCT-116 human colon carcinoma cell. One active fraction was identified with peptide signals, using 1H-NMR analysis and MS analysis to reveal two separate peaks with [M+H] of 1098.7 and 1112.7, respectively (FIG. 2). We purified both compounds using semi-preparative HPLC and both of them showed strong cytotoxic activity at a concentration of 0.074 ng/ml. Using NMR, MS and the AntiMarin database, we identified these two compounds to be didemnin B and nordidemnin B. A pure form didemnin B was acquired from Prof. Chris Ireland at the University of California, Santa Cruz, who with coworkers, isolated it from the tunicate Trididemnum solidum. This sample was used to compare its NMR spectral data with the present data and to further confirm that the compound isolated from T. mobilis was didemnin B. Moreover, other fractions from T. mobilis also exhibited similar bioactivity and LC-MS analysis showed the presence of other didemnins, including didemnin A, and didemnin C, at trace amounts. This is consistent with the fact that didemnin B and nordidemnin B were the most abundant in the Trididemnum tunicates.
Biosynthetic Pathways of Didemnins
The putative biosynthetic gene cluster for didemnins contains 26 ORFs (Table 2).







TABLE 2







Deduced functions of the proteins in the didemnin biosynthetic gene cluster













Amino


Identity/
GenBank


gene
acid aa)
Sequence similarity/organism
Proposed function
similarity
accession no.















orf1
261
thioesterase, Actinomadura kijaniata
Thioesterase
41%, 54%
ACB46473


orf2
483
band 7 protein, Methylobacter

50%, 68%
ZP_07653046




tundripaludum SV96


orf3
560
cyclic peptide transporter,
transporter
48%, 68%
ZP_07653047




Methylobacter tundripaludum SV96


orf4
70
No hits


orf5
190
GTPase domain-containing protein,

39%, 57%
ZP_07653048




Methylobacter tundripaludum SV96


orf6
987
hydrophobic/amphiphilic exporter-1,
Resistance
38%, 56%
YP_003450508




Azospirillum sp. B510


orf7
377
secretion protein, Azospirillum sp.
Resistance
28%, 47%
YP_003450507




B510


didA
2123
OciA protein, Planktothrix rubescens
NRPS (C A T C A
29%, 40%
CAQ48254




NIVA-CYA 98
T)


didB
1796
linear gramicidin synthetase subunit D,
NPRS (C A KR T)
36%, 48%
ZP_01459555




Stigmatella aurantiaca DW4/3-1


didC
1330
NRPS, Myxococcus xanthus DK 1622
NRPS (C A T C*)
41%, 52%
YP_632257


didD
3853
amino acid adenylation domain protein,
NRPS (C A M T
39%, 50%
ZP_07603194




Streptomyces violaceusniger Tu 4113
C* C A T C A T)


didE
1705
amino acid adenylation domain protein,
NRPS/PKS (KS
36%, 53%
ZP_07325073




Acetivibrio cellulolyticus CD2
KR T C)


didF
1613
HctF, Lyngbya majuscula
NRPS (A* A KR
35%, 52%
AAY42398





T)


didG
1413
NRPS/PKS, Amycolatopsis
PKS (KS M T)
46%, 56%
YP_003765866




mediterranei U32


didH
1286
NRPS/PKS, Myxococcus xanthus DK
NRPS (C A T C*)
39%, 53%
YP_631961




1622


didI
873
NRPS, Myxococcus xanthus DK 1622
NRPS (C A T)
39%, 52%
YP_632257


didJ
2163
amino acid adenylation domain protein,
NRPS (C A M M T
36%, 53%
ZP_08431746




Lyngbya majuscula 3L
TE)


orf8
77
MbtH domain-containing protein,
MbtH-like protein
80%, 89%
YP_001542806




Herpetosiphon aurantiacus ATCC




23779


orf9
68
hypothetical protein, Acidovorax sp.

79%, 91%
YP_986866




JS42


orf10
45
No hits


orf11
60
No hits


orf12
190
hypothetical protein, Acidovorax sp.

94%, 97%
YP_986861




JS42


orf13
75
hypothetical protein, Acidovorax sp.

98%, 100%
YP_004387524




JS42


orf14
324
CAAX amino terminal protease family,

30%, 49%
ZP_05035401




Synechococcus sp. PCC 7335


orf15
398
cyanate transport system protein,

40%, 52%
ZP_07265073




Pseudomonas syringae pv. syringae




642


orf16
255
GntR family transcriptional regulator,
Regulation
39%, 55%
NP_903400




Chromobacterium violaceum ATCC




12472









Ten of them, didA to didJ, are most likely to be involved in the biosynthesis of didemnins (FIG. 3). All of them encode for non-ribosomal peptide synthetase (NRPS) except didE and didG. Most NRPS are modular enzymes that use an assembly line strategy to synthesize nonribosomal peptides. A typical module in the NRPS assembly line usually has a minimal of three domains: the adenylation domain (A) activates a specific amino acid via the formation of an acyl-AMP; the activated form of the amino acid is then loaded to an adjacent thiolation (T) domain; and the condensation (C) domain catalyzes peptide bond formation. In addition to these three basic domains, different tailoring domains, such as ketoreductase (KR) domains or methyltransferase (MT) domains, are sometimes present in NRPS modules. These tailoring domains make modification of the amino acids and they are one of the main reasons that many non-ribosomal peptides contain non-proteinogenic amino acid momoners. Most NRPS generally follow a colinearity principle wherein the A domain specificity and placement within the NRPS assembly line dictates the sequence of the monomers in the nonribosomal peptide (Marahiel et al. 1997; Fischbach and Walsh 2006). Analysis of the substrate specificity of the A domains by NPRSpredictor can substantially help match the A domains with the corresponding amino acid substrate. There are a total of 11 A domains in the eight NRPS in the didemnin gene clusters and it is the specificity of these A domains that leads us to postulate that this gene cluster is responsible for didemnin synthesis (Table 3).















Adenyla-






tion
10 amino 

Identity,


protein
domain
acid code
Substrate
%



















DidA
A1
DAWQFGLIDK
Glutamine
100




(SEQ ID NO: 1)




 


DidA
A2
DAWQFGLIDK
Glutamine
100




(SEQ ID NO: 1)




 


DidB
A3
DHPWIAETVK
(pyruvic 





(SEQ ID NO: 2)
acid)



 


DidC
A4
DVQFAAQVVK
Proline
90




(SEQ ID NO: 3)




 


DidD
A5
DAWFLGHVVK
Leucine
90




(SEQ ID NO: 4)




 


DidD
A6
DFWNIGMVHK
Threonine
100




(SEQ ID NO: 5)




 


DidD
A7
DAFFLGITFK
Ile
90




(SEQ ID NO: 6)




 


DidF
A8
No code
(2-oxoiso-






valeric






acid)



 


DidH
A9
DAWFLGNVVK
Leucine 
100




(SEQ ID NO: 7)




 


DidI
A10
DVQFAAQVVK
Proline 
90




(SEQ ID NO: 8)




 


DidJ
A11
DASTLAAVCK
Tyrosine
90




(SEQ ID NO: 9)









For example, the inventors found that in at least certain aspects the A domain of DidC activates proline while the first two A domains in DidD activate leucine and threonine, respectively. A fragment of the didemnin B happens to contain these three amino acids in the same sequence as they are activated by the corresponding A domains. Moreover, the leucine in the didemnin B has an N-terminal methyl group and this matches well with the fact that there is also a methylation domain right next to the A domain activating leucine. In a similar way, the inventors found the A domains in DidH, DidI and DidJ in certain embodiments activate leucine, proline and tyrosine. DidI contains a MT tailoring domain that will convert proline into N-methyl proline while DidJ contains two MT domains, one N-methyltransferase and one O-methyltransferase, which may respectively add a methyl group on the amine and hydroxyl group of the tyrosine. Leucine, N-methyl proline and N,O-dimethyl tyrosine together make another fragment of the didemnin B.
The remaining monomers of the didemnin B are a lactic acid (Lac), an isostatine (Ist) and an α-(α-hydroxyisovaleryl)-propionic acid (Hip). According to the colinearity rule, DidB, which contains four domains (C-A-KR-T), should be the module that feeds the lactic acid into didemnin B. Although NRPSpredictor fails to predict a reliable substrate for the didB A domain, BLAST search of its sequence shows that it is highly similar to one of the A domains found in the valinomycin gene cluster. According to Chen (2006), this A domain may activate pyruvic acid. The pyruvic acid is then reduced to lactic acid, which is also a monomer of valinomycin. Therefore, the A domain in DidB may first activate pyruvic acid and the KR domain then comes to reduce the pyruvic acid to lactic acid, which is exactly the same scenario in the vancomycin gene cluster. The third A domain in DidD is most likely to activate isoleucine according to the code specificity analysis. Downstream to DidD is a hybrid polyketide sythetase/nonribosomal protein synthetase (PKS/NRPS) protein DidE comprising of a Ketosynthase domain (KS), a Ketoreductase (KR), a thiolation domain (T) and a condensation domain (C). It can be hypothesized that the after isoleucine is incorporated, the intermediate molecule bearing the isoleucine goes through a round of PKS reaction which contains two steps: 1) addition of a 2-carbon unit as a common PKS chain elongation step possibly using a malonyl-CoA as a substrate, and 2) the carbonyl group of the isoleucine is reduced to a hydroxyl group. This series of reactions can then explain the existence of the monomer Ist in the didemnin B molecule. However, the above PKS system features as the “AT-less” PKS system as no acyltransferase can be found in this module. Moreover, no stand alone AT genes can be found in the entire didemnin gene cluster and its vicinity, which is a very rare phenomenon in “AT-less” PKS systems as normally at least one stand alone AT gene can be found in the vicinity that functions in trans to catalyze the activation of the substrate, which most of the time would be a malonyl-CoA. Nevertheless, in certain aspects the Ist monomer is added through the joint effect of a NRPS module and a hybrid PKS/NPRS module. It is unlikely that a single step in which an A domain specifically activates Ist catalyzes the reaction as predicted by Salomon et al. (2004). Downstream of the didD gene are didE and didF, the former is a NRPS gene while the latter is a PKS gene. The A domain in DidE does not have a code specified for any known substrate, yet its amino acid sequence shares 43% identity and 60% similarity with that of the first adenylation domain of HetE of the cyanobacterium Lyngbya majuscule that produces the cyclic peptide hectochlorin. Ramaswamy et al. (2007) used the ATP-PPi exchange activity assay to demonstrate that the first A domain of HetE can activate 2-oxo-isovaleric acid. Therefore the A domain in DidE may first activate the 2-oxo-isovaleric acid, which is then reduced in situ by a KR domain in DidE to 2-hydroxyisovaleric acid (2-Hiv). The KS domain of DidF then adds a 2-carbon unit to the C-terminus of Hiv and the MT domain in DidF catalyzes the addition of a methyl group. The net outcome of this cascade of reactions is the incorporation of the monomer Hip into didemnin molecules (FIG. 4). This is very similar to the scenario of the addition of Ist in which a joint effort of two proteins eventually catalyze the addition of the necessary monomer into the final molecule. Finally, in some aspects the thioesterase domain of the DidJ catalyzes the macrocyclization between the hydroxyl group on the side chain of threonine residue and the thioester group of T domain bound tyrosine residue, resulting in the release of the mature cyclic depsipeptide didemnin compounds. Therefore, in certain embodiments of the invention didB to didJ are responsible for the biosynthesis of didemnin B (FIG. 5).
Apart from didemnin B, the inventors also isolated nordidemnin B, which contains the monomer norstatine, a very similar derivative of isostatine. Since the A domains usually have some flexibility in activating similar substrates, valine, instead of isoleucine, may also be activated by the third A domain of DidD and together with DidE, norstatine may therefore be incorporated to produce nordidemnin B, in some embodiments. Upstream of didB, didA encodes two modules of NRPS with the C-A-T-C-A-T domain organization. Both A domains have specified codes to activate glutamine. There are some other didemnins which have multiple glutamine residues preceding the lactic residue, such as didemnin X (FIG. 1) and didemnin Y. In some cases with didA, this gene cluster produces didemnin X or didemnin Y. Similar to plipastatin, didemnin X and didemnin Y are both N-acylated. It has been reported that the first C domain in the initiation module may catalyze the N-acylation of the first amino acid in some NRPS systems (Imker et al. 2010). However, further studies are performed to characterize this. Apart from didemnin B and nordidemnin B, the inventors also used UPLC-HRMS to detect many other didemnin derivatives, such as didemnin A and didemnin C by growing T. mobilis in different culture media or with different culture durations.
Additional ORFs in the Didemnin Gene Cluster
Besides the synthesis of didemnins, other genes that are possibly involved in the regulation, resistance and transport of the didemnins are also found in the didemnin gene cluster. Orf1, which encodes a type II thioesterase, may regenerate the misprimed NPRS which are inactive to ensure the NRPS assembly line is functional. Orf3, orf6 and orf7 all encode proteins related to the transport and secretion of the cyclic peptide and they may be responsible for the secretion of didemnins. As secretion and self-resistance are often related, they may also function as self-resistance genes to protect the producer. Orf8 encodes an MbtH-like protein that is a common resident of various NRPS systems. Although the precise function of MbtH-like proteins in NRPS systems has not been revealed, in specific aspects they play an important role in the production of many non-ribosomal peptides as indicated by Lautru et al., (2007). Orf16 encodes a Gnt-family transcription factor and this is the only transcription-related gene that can be found in the vicinity of the didemnin biosynthetic gene cluster. As GntR family transcription factors usually, though not always act as repressive regulators (Chen et al, 2010), orf16 may participate in the regulation of the biosynthesis of didemnins. It is also reasonable as overproduction of complicated didemnins could cause a huge waste of energy for the bacterium. Although didemnins have been reported for their potent cytotoxic, antivirus, immunosuppressive activities, the real ecological function of these compounds for the producing bacterium is unknown. To synthesize such complicated molecules, the bacterium has to invest considerable energy with good reason.
Manipulation of the Didemnin Gene Cluster
There are huge incentives to manipulate the didemnin gene cluster as didemnins other than didemnin B or nordidemnin B could be produced from this gene cluster. For example, there might be a way to produce dehydrodidemnin B (Aplidine), which is a promising antitumor drug currently in clinical trials (Le Tourneau et al. 2010, Mateos et al. 2010). The only difference between dehydrodidemnin B and didemnin B is that the former has a pyruvic acid monomer while the latter has a lactic acid. If the ketoreductase domain in didB can be knocked out, then the incorporated pyruvic acid will not be reduced to lactic acid and consequently, dehydrodidemnin B may be expected to be produced. Upon construction of the corresponding deletion mutant, there may be an alternative way to produce dehydrodidemnin B other than chemical synthesis, and the cost could be substantially reduced. In addition, as more than 10 mg/L didemnin B and nordidemnin B can be harvested from fermentation of Tistrella mobilis using a simple medium for as short as 3 days, improvement of the culture conditions may further increase the production of the didemnins. Because of their large molecular weight and high lipophilicity, purification of the didemnins is quite simple by chromatographic separation, for example, which in turn can provide the starting material to semisynthesize dehydrodidemnin B. Furthermore, genetic engineering of the didemnin gene cluster provides novel didemnin compounds with improved biological activities.
Example 3
Other Didemnins Produced by Tistrella Mobilis 
In particular aspects of the invention, there are didemnins other than didemnin B and nordidemninB from Tistrella mobilis. As shown in FIGS. 6, 7A, and 7B using UPLC-HRMS, there was detection of some other didemnins such as didemnin A, nordidemnin A and didemnin N. In certain aspects, there are new didemnins with similar molecular weights but different from all reported didemnins (see FIGS. 8A and 8B). They also have similar UV absorption patterns to didemnins.
















Molecular




Name
Formula
weight
Reference
Source



















Didemnin A
C49H78N6O12
943.177
Crampton, S. L. et al., Cancer Res., 44 (1984) 1796; M.B.
Trididemnum sp.





Hossain et al., Internat. J. Peptide Protein Res. 47 (1996)





20-27


Didemnin B
C57H89N7O15
1,112.35
Crampton. S. L. et al., Cancer Res., 44 (1984) 1796;
Trididemnum sp.


Didemnin C
C52H82N6O14
1,015.24
Rinehart, K. L. et al., Science, 212 (1981) 933;
Trididemnum spp.


Prolyldidenmin A
C54H85N7O13
1,040.29
Schmidt, U. et al., Tetrahedron Lett., 29, 4407-8 1988


didemnin D
C77H118N14O23
1,607.84
Rinehart KL Kishore V Bible KC Sakai R Sullins DW Li
Chordata Trididemnum





K-M J. Nat. Prod. 1988 51 1-21
solidum


didemnin E
C72H110N12O21
1,479.71
Rinehart KL Kishore V Bible KC Sakai R Sullins DW Li
Chordata Trididemnum





K-M J. Nat. Prod. 1988 51 1-21
solidum


didemnin G
C58H89N7O16
1,140.36
K. L. Rinehart, 2nd Euroconf. Marine Nat. Prod., 1999
Trididemnum solidum





Santiago d. Comp.,


isodidemnin-1
C57H89N7O15
1,112.35
Guyot M Davoust D Morel E C. R. Acad. Sci. P Ser. II
Chordata Trididemnum





1987 305 681-686
cyanophorum


nordidemnin B
C56H87N7O15
1,098.33
McKee TC Ireland CM Lindquist N Fenical W
Chordata Trididemnum





Tetrahedron Lett. 1989 30 3053-3056
solidum


didemnin M
C67H102N10O19
1,351.58
Sakai R Stroh JG Sullins DW Rinehart KL J. Am. Chem.
Chordata Trididemnum





Soc. 1995 117 3734-3748
solidum


didemnin N
C55H85N7O15
1,084.30
Sakai R Stroh JG Sullins DW Rinehart KL J. Am. Chem.
Chordata Trididemnum





Soc. 1995 117 3734-3748
solidum


didemnin X
C82H131N13O23
1,666.99
Sakai R Stroh JG Sullins DW Rinehart KL J. Am. Chem.
Chordata Trididemnum





Soc. 1995 117 3734-3748
solidum


didemnin Y
C87H139N15O25
1,795.12
Sakai R Stroh JG Sullins DW Rinehart KL J. Am. Chem.
Chordata Trididemnum





Soc. 1995 117 3734-3748
solidum


nordidemnin N
C54H83N7O15
1,070.27
Sakai R Stroh JG Sullins DW Rinehart KL J. Am. Chem.
Chordata Trididemnum





Soc. 1995 117 3734-3748
solidum


epididemnin A1
C49H78N6O12
943.177
Sakai R Stroh JG Sullins DW Rinehart KL J. Am. Chem.
Chordata Trididemnum





Soc. 1995 117 3734-3748
solidum


acyclodidemnin A
C49H80N6O13
961.192
Sakai R Stroh JG Sullins DW Rinehart KL J. Am. Chem.
Chordata Trididemnum





Soc. 1995 117 3734-3748
solidum


[tyr5]didemnin B
C55H85N7O15
1,084.30
Aboumansour E Boulanger A Badre A Bonnard I Banaigs B
Chordata Trididemnum





Combaut G Francisco C Tetrahedron 1995 51 12591-12600
cyanophorum


[D-pro4]didemnin
C57H89N7O15
1,112.35
Aboumansour E Boulanger A Badre A Bonnard I Banaigs B
Chordata Trididemnum


B


Combaut G Francisco C Tetrahedron 1995 51 12591-12600
cyanophorum


didemnin H
C67H102N10O19
1,351.58
Boulanger A Abou-Mansour E Badre A Banaigs B
Chordata Trididemnum





Combaut G Francisco C Tetrahedron Lett. 1994 35 4345-4348
cyanophorum


dehydrodidemnin
C57H87N7O15
1,110.34
Rinehart KL Lithgow-Bertelloni AM Ruffles GK PCT
Chordata Aplidium


B


Int. Appl. 1991 0 0
albicans


[Hysp2]didemnin
C58H91N7O15
1,126.38
Banaigs B Mansour EA Bonnard I Boulanger A Francisco C
Chordata Aplidium


B


Tetrahedron 1999 55 9559-9574
albicans, Chordata






Trididemnum






cyanophorum, Chordata






Trididemnum solidum


[Hap2]didemnin
C54H83N7O15
1,070.27
Banaigs B Mansour EA Bonnard I Boulanger A Francisco C
Chordata Aplidium


B


Tetrahedron 1999 55 9559-9574
albicans, Chordata






Trididemnum






cyanophorum, Chordata






Trididemnum solidum









Example 4
Sequence Information
The following table provides annotation of the Tistrella mobilis genome (including plasmids 1-4 (P1-P4)).
















Locus
Gene
Product
Start
End
Strand
Length





















P1.orf0001
dhbF
Amino acid adenylation
1
3501
1
3501


P1.orf0002

HNH endonuclease domain-containing protein
3566
4135
2
570


P1.orf0003

conserved hypothetical protein
4297
4632
1
336


P1.orf0004

conserved hypothetical protein
4675
4920
1
246


P1.orf0005

conserved hypothetical protein
4910
5203
2
294


P1.orf0006

Tetratricopeptide TPR_4
5622
7493
3
1872


P1.orf0007

hypothetical protein
7712
7870
2
159


P1.orf0008

2-nitropropane dioxygenase NPD
10299
9328
−1
972


P1.orf0009

cytochrome B561
10869
10303
−1
567


P1.orf0010

TPR repeat-containing protein
11116
12300
1
1185


P1.orf0011
arcB
ornithine cyclodeaminase
13363
12311
−2
1053


P1.orf0012
arcB
arginase
14343
13360
−1
984


P1.orf0013

hypothetical protein
15282
14491
−1
792


P1.orf0014

transcriptional regulator
15452
15904
2
453


P1.orf0015

D-aminoacylase
16007
17512
2
1506


P1.orf0016
cobW
Cobalamin biosynthesis protein CobW
17509
18474
1
966


P1.orf0017
kipR
transcriptional regulator, IclR family
18495
19313
3
819


P1.orf0018

transposase
19680
19988
3
309


P1.orf0019

transposase, IS4
20131
20361
1
231


P1.orf0020
recF
DNA replication and repair protein recF
20617
22251
1
1635


P1.orf0021
yagA
integrase catalytic subunit
24237
23488
−1
750


P1.orf0022
dadA
D-amino-acid dehydrogenase (DadA-like)
25991
24768
−3
1224


P1.orf0023
gsiA
putative ABC transporter, ATP-binding protein
27732
26053
−1
1680


P1.orf0024

putative ABC transporter, permease protein
28582
27737
−2
846


P1.orf0025

putative ABC transporter, permease protein
29522
28803
−3
720


P1.orf0026

binding-protein-dependent transport systems
28809
28582
−1
228




inner membrane component


P1.orf0027

putative ABC transporter, periplasmic binding
31211
29595
−3
1617




protein


P1.orf0028
baeS
two component sensor histidine kinase, AdeS
32657
31479
−3
1179


P1.orf0029

putative transcriptional regulator ycf27
33415
32654
−2
762


P1.orf0030

conserved hypothetical protein
33688
34317
1
630


P1.orf0031

conserved hypothetical protein
34400
35098
2
699


P1.orf0032

conserved hypothetical protein
36007
35186
−2
822


P1.orf0033

hypothetical protein
36386
36081
−3
306


P1.orf0034

Sulfatase-modifying factor
36553
37887
1
1335


P1.orf0035

Meiotically up-regulated gene 158 protein
37915
38916
1
1002


P1.orf0036

putative transcriptional regulator
39030
39296
3
267


P1.orf0037

conserved hypothetical protein
39293
39718
2
426


P1.orf0038

conserved hypothetical protein
40110
39703
−1
408


P1.orf0039

conserved hypothetical protein
40969
40127
−2
843


P1.orf0040

hypothetical protein
41084
40953
−3
132


P1.orf0041

conserved hypothetical protein
41113
41679
1
567


P1.orf0042

conserved hypothetical protein
41718
42971
3
1254


P1.orf0043
yciE
conserved hypothetical protein
42989
44128
2
1140


P1.orf0044

TPR repeat
45084
44143
−1
942


P1.orf0045

conserved hypothetical protein
45365
45081
−3
285


P1.orf0046

aspartyl/asparaginyl beta-hydroxylase
46396
45362
−2
1035


P1.orf0047
asnB
Asparagine synthase (glutamine-hydrolyzing)
48329
46389
−3
1941


P1.orf0048

hypothetical protein
48512
48342
−3
171


P1.orf0049

transcriptional regulator, AsnC family
49221
48742
−1
480


P1.orf0050

conserved hypothetical protein
49373
50158
2
786


P1.orf0051

L-carnitine dehydratase/bile acid-inducible
51309
50179
−1
1131




protein F


P1.orf0052
exoD
putative exopolysaccharide synthesis protein
51530
52174
2
645


P1.orf0053

sensory box/GGDEF family protein
54320
52131
−3
2190


P1.orf0054

PEBP family protein
54541
56367
1
1827


P1.orf0055

hypothetical protein
56514
56780
3
267


P1.orf0056

inositol-1-monophosphatase
56949
57809
3
861


P1.orf0057

hypothetical protein
58072
57815
−2
258


P1.orf0058
feaR
AraC family transcriptional regulator
58256
59380
2
1125


P1.orf0059

hypothetical protein
59582
59920
2
339


P1.orf0060

hypothetical protein
60233
59958
−3
276


P1.orf0061

conserved hypothetical protein
60341
60535
2
195


P1.orf0062

hypothetical protein
61819
60983
−2
837


P1.orf0063

conserved hypothetical protein
62520
61816
−1
705


P1.orf0064

hypothetical protein
63257
62658
−3
600


P1.orf0065

hypothetical protein
63988
63254
−2
735


P1.orf0066

conserved hypothetical protein
65137
64061
−2
1077


P1.orf0067

conserved hypothetical protein
66168
65134
−1
1035


P1.orf0068

vgrG protein
68924
66195
−3
2730


P1.orf0069

conserved hypothetical protein
69570
69058
−1
513


P1.orf0070

conserved hypothetical protein
71219
69567
−3
1653


P1.orf0071

ABC transporter, permease protein
72438
71212
−1
1227


P1.orf0072
macB
Macrolide export ATP-binding/permease
73142
72438
−3
705




protein


P1.orf0073

ppkA-related protein
75115
73139
−2
1977


P1.orf0074

serine/threonine kinase
76542
75115
−1
1428


P1.orf0075
prpC
Ser/Thr protein phosphatase
77305
76535
−2
771


P1.orf0076

conserved hypothetical protein
78018
77302
−1
717


P1.orf0077

conserved hypothetical protein
81725
78000
−3
3726


P1.orf0078
ytxE
conserved hypothetical protein
83263
81725
−2
1539


P1.orf0079

conserved hypothetical protein
84610
83267
−2
1344


P1.orf0080

type VI secretion lipoprotein, VC_A0113 family
85178
84669
−3
510


P1.orf0081

FHA domain-containing protein
86617
85211
−2
1407


P1.orf0082

type VI secretion ATPase, ClpV1 family
89264
86661
−3
2604


P1.orf0083

type VI secretion system protein ImpH
90365
89334
−3
1032


P1.orf0084

type VI secretion system protein ImpG
92216
90375
−3
1842


P1.orf0085

type VI secretion system protein ImpF
92731
92213
−2
519


P1.orf0086

type VI secretion system protein ImpE
93597
92728
−1
870


P1.orf0087

type VI secretion system protein ImpC
95063
93663
−3
1401


P1.orf0088

type VI secretion system protein ImpC
96576
95080
−1
1497


P1.orf0089

type VI secretion protein
97115
96582
−3
534


P1.orf0090

impA-related N-terminal protein
98317
97175
−2
1143


P1.orf0091

peptidase S1 and S6, chymotrypsin/Hap
99083
98634
−3
450


P1.orf0092
hcp1
conserved hypothetical protein
99418
99891
1
474


P1.orf0093

hypothetical protein
100070
100681
2
612


P1.orf0094

hypothetical protein
100695
101285
3
591


P1.orf0095

VgrG protein
101290
103272
1
1983


P1.orf0096

hypothetical protein
103322
104083
2
762


P1.orf0097

conserved hypothetical protein
104130
104543
3
414


P1.orf0098
rhsC
putative rhs-related transmembrane protein
104548
109017
1
4470


P1.orf0099

conserved hypothetical protein
109034
109873
2
840


P1.orf0100

conserved hypothetical protein
109983
110342
3
360


P1.orf0101

Ribosomal protein L7/L12 C-terminal domain.
110467
110853
1
387


P1.orf0102

hypothetical protein
110962
111171
1
210


P1.orf0103

hypothetical protein
111187
111699
1
513


P1.orf0104

conserved hypothetical protein
111745
112572
1
828


P1.orf0105

conserved hypothetical protein
112700
113104
2
405


P1.orf0106

conserved hypothetical protein
113104
113838
1
735


P1.orf0107
treY
malto-oligosyltrehalose synthase
113999
116740
2
2742


P1.orf0108

lipolytic enzyme
117759
116767
−1
993


P1.orf0109
paiB
transcriptional repressor of sporulation and
117861
118490
3
630




degradative enzyme production


P1.orf0110

Metallophosphoesterase
119313
118522
−1
792


P1.orf0111
ywfA
putative transporter protein
119527
120717
1
1191


P1.orf0112
ybfI
AraC family transcriptional regulator
120779
121609
2
831


P1.orf0113

conserved hypothetical protein
121817
121620
−3
198


P1.orf0114

conserved hypothetical protein
122207
121902
−3
306


P1.orf0115

putative addiction module antidote protein,
122540
122764
2
225




CopG/Arc/MetJ family


P1.orf0116

plasmid stabilization system protein
122765
123076
2
312


P1.orf0117

conserved hypothetical protein
123161
124144
2
984


P1.orf0118
ywjA
ABC transporter, nucleotide binding/ATPase
125820
124108
−1
1713




protein


P1.orf0119
fhuB
transport system permease protein
126404
125895
−3
510


P1.orf0120
fhuB
transport system permease protein
127864
126395
−2
1470


P1.orf0121
fhuD
Iron(3+)-hydroxamate-binding protein fhuD
128711
127857
−3
855


P1.orf0122
fhuC
iron-hydroxamate transporter ATP-binding
129528
128731
−1
798




subunit


P1.orf0123
fhuA
ferrichrome receptor precursor protein
131773
129539
−2
2235


P1.orf0124
msmR
transcriptional regulator, AraC family
132821
131928
−3
894


P1.orf0125

Regulator of cell morphogenesis and NO
133622
132918
−3
705




signaling


P1.orf0126

GCN5-related N-acetyltransferase
134591
133734
−3
858


P1.orf0127
appF
putative dipeptide ABC transporter, ATP-
135640
134654
−2
987




binding protein


P1.orf0128

Oligopeptide/dipeptide transporter domain
136449
135637
−1
813




family protein


P1.orf0129
ddpC
ABC transporter permease protein 1
137342
136446
−3
897


P1.orf0130
ddpB
binding-protein dependent transport system
138361
137339
−2
1023




inner membrane protein


P1.orf0131
hbpA
extracellular solute-binding protein family 5
140053
138434
−2
1620


P1.orf0132
soxC
DszC-like desulfurization enzyme
141285
140104
−1
1182


P1.orf0133
soxA
flavin-dependent oxidoreductase
142712
141282
−3
1431


P1.orf0134
hcaR
transcriptional regulator, LysR family
144315
143398
−1
918


P1.orf0135

short-chain dehydrogenase/reductase SDR
144452
145222
2
771


P1.orf0136
catD
carboxylesterase
146146
145223
−2
924


P1.orf0137

conserved hypothetical protein
146388
147218
3
831


P1.orf0138

acyl-CoA dehydrogenase
147267
148451
3
1185


P1.orf0139

hypothetical protein
146396
146217
−3
180


P1.orf0140

acyl-CoA dehydrogenase family protein
148483
149682
1
1200


P1.orf0141

hypothetical protein
150210
149764
−1
447


P1.orf0142
parA
Cobyrinic acid ac-diamide synthase
151061
150207
−3
855


P1.orf0143

hypothetical protein
151289
151116
−3
174


P1.orf0144

transcriptional regulator, CopG family
151688
151413
−3
276


P1.orf0145
mtnC
2,3-diketo-5-methylthio-1-phosphopentane
152572
151757
−2
816




phosphatase


P1.orf0146
mtnD
acireductone dioxygenase ARD
153107
152562
−3
546


P1.orf0147
mtnB
methylthioribulose-1-phosphate dehydratase
153733
153104
−2
630


P1.orf0148

replication protein A
156147
157436
3
1290


P1.orf0149

hypothetical protein
156098
155670
−3
429


P1.orf0150

hypothetical protein
158775
159935
3
1161


P1.orf0151

conserved hypothetical protein
159979
166170
1
6192


P1.orf0152
dctB
two-component sensor histidine kinase protein
166326
168158
3
1833


P1.orf0153
dctD
two component, sigma54 specific,
168155
169513
2
1359




transcriptional regulator, Fis family


P1.orf0154

TRAP transporter solute receptor, TAXI family
169676
170662
2
987




protein


P1.orf0155

TRAP transporter, 4TM/12TM fusion protein
170765
172939
2
2175


P1.orf0156

3-methylcrotonoyl-CoA carboxylase beta
174655
173048
−2
1608




subunit


P1.orf0157
Pcca
putative acyl-CoA carboxylase, Biotin/lipoyl
175185
174652
−1
534




carrier domain


P1.orf0158
pycA
acetyl/propionyl CoA carboxylase alpha
176739
175264
−1
1476




subunit


P1.orf0159
citE
Citryl-CoA lyase
177666
176746
−1
921


P1.orf0160

dehydratase
178133
177663
−3
471


P1.orf0161

short-chain dehydrogenase/reductase SDR
178304
179137
2
834


P1.orf0162
IVD
Isovaleryl-CoA dehydrogenase
180424
179222
−2
1203


P1.orf0163
IivF
ABC transporter related protein
181236
180448
−1
789


P1.orf0164

putative branched-chain amino acid transport
182524
181244
−2
1281




system substrate-binding protein


P1.orf0165
braE
inner-membrane translocator
183668
182595
−3
1074


P1.orf0166
braD
inner-membrane translocator
184561
183674
−2
888


P1.orf0167

AMP-dependent synthetase and ligase
186533
184563
−3
1971


P1.orf0168
braF
ABC transporter related protein
187335
186523
−1
813


P1.orf0169
fadR
TetR family transcriptional regulator
188519
187803
−3
717


P1.orf0170

hypothetical protein
188784
189266
3
483


P1.orf0171
tetR
Tetracycline repressor protein class H
189987
189301
−1
687


P1.orf0172
eutQ
Ethanolamine utilization protein eutQ
190133
190513
2
381


P1.orf0173
ordL
FAD dependent oxidoreductase
190510
191829
1
1320


P1.orf0174
yiiZ
deoxycytidine triphosphate (dCTP) deaminase
191903
192943
2
1041


P1.orf0175

TRAP-type C4-dicarboxylate transport system
192995
193501
2
507




small permease component


P1.orf0176
siaT
putative DctM (C4-dicarboxylate permease,
193503
194792
3
1290




large subunit)


P1.orf0177

hypothetical protein
195002
195325
2
324


P1.orf0178

conserved hypothetical protein
195425
195892
2
468


P1.orf0179

TetR family transcriptional regulator
196517
195846
−3
672


P1.orf0180
yesF
oxidoreductase yesF
196619
197461
2
843


P1.orf0181
calB
aldehyde dehydrogenase
198848
197430
−3
1419


P1.orf0182

TetR family transcriptional regulator
199683
199045
−1
639


P1.orf0183

conserved hypothetical protein
199899
200906
3
1008


P1.orf0184

putative pyridoxine 5′-phosphate oxidase
201927
201238
−1
690


P1.orf0185

LysR family transcriptional regulator
202044
202982
3
939


P1.orf0186

conserved hypothetical protein
204005
202992
−3
1014


P1.orf0187

conserved hypothetical protein
204591
203998
−1
594


P1.orf0188
cydB
cytochrome d ubiquinol oxidase, subunit II
206024
204870
−3
1155


P1.orf0189
cydA
cytochrome D ubiquinol oxidase, subunit I
207644
206031
−3
1614


P1.orf0190
cydC
transport ATP-binding protein CYDC
209417
207732
−3
1686


P1.orf0191
cydD
ABC transporter, CydDC cysteine exporter
211018
209444
−2
1575




(CydDC-E) family, permease/ATP-binding




protein CydD


P1.orf0192

hypothetical protein
211079
211204
2
126


P1.orf0193
dcrA
methyl-accepting chemotaxis protein
211329
213422
3
2094


P1.orf0194

putative araC-like transcription regulator
214364
213435
−3
930


P1.orf0195
yusZ
short-chain dehydrogenase/reductase SDR
214465
215220
1
756


P1.orf0196
ubiE
Methyltransferase type 11
216154
215240
−2
915


P1.orf0197

major facilitator transporter
217467
216211
−1
1257


P1.orf0198
fyuA
TonB-dependent receptor
219740
217473
−3
2268


P1.orf0199
pchR
regulatory protein Pchr
220674
219814
−1
861


P1.orf0200

hypothetical protein
221164
220736
−2
429


P1.orf0201

hypothetical protein
221470
221198
−2
273


P1.orf0202

transposase, IS4
222251
221904
−3
348


P1.orf0203

PilT domain-containing protein
222699
222268
−1
432


P1.orf0204

conserved hypothetical protein
222905
222696
−3
210


P1.orf0205

conserved hypothetical protein
223604
223110
−3
495


P1.orf0206
dhkJ
multi-sensor hybrid histidine kinase
228426
223648
−1
4779


P1.orf0207
ybaR
sulphate transporter
229028
230536
2
1509


P1.orf0208

putative addiction module antidote protein,
230716
230997
1
282




CopG/Arc/MetJ family


P1.orf0209

hypothetical protein
231751
231569
−2
183


P1.orf0210

hypothetical protein
232640
232413
−3
228


P1.orf0211
malQ
glycoside hydrolase family protein
234541
232637
−2
1905


P1.orf0212
glgX
glycogen debranching enzyme GlgX
238557
234628
−1
3930


P1.orf0213

glycogen branching enzyme
240732
238567
−1
2166


P1.orf0214
treS
trehalose synthase
244194
240808
−1
3387


P1.orf0215
aam1
alpha amylase catalytic region
247202
244200
−3
3003


P1.orf0216

hypothetical protein
247741
247244
−2
498


P1.orf0217

hypothetical protein
247736
247966
2
231


P1.orf0218

cyclase/dehydrase
248034
248840
3
807


P1.orf0219
otsB
trehalose-phosphatase
248837
249631
2
795


P1.orf0220
otsA
alpha,alpha-trehalose-phosphate synthase
249734
251275
2
1542




(UDP-forming)


P1.orf0221

TRAP dicarboxylate transporter, DctM subunit
252686
251367
−3
1320


P1.orf0222

TRAP dicarboxylate transporter, DctQ subunit
253240
252683
−2
558


P1.orf0223
yiaO
TRAP dicarboxylate family transporter, DctP
254661
253585
−1
1077




subunit


P1.orf0224
mvaA
hydroxymethylglutaryl-CoA reductase,
256027
254723
−2
1305




degradative


P1.orf0225
ydcR
GntR family transcriptional regulator
256219
257616
1
1398


P1.orf0226

AraC family transcriptional regulator
258898
257852
−2
1047


P1.orf0227

Extracellular ligand-binding receptor
259239
260420
3
1182


P1.orf0228
IivH
inner-membrane translocator
260507
261364
2
858


P1.orf0229
IivM
inner-membrane translocator
261376
262317
1
942


P1.orf0230
braF
ABC transporter related protein
262314
263063
3
750


P1.orf0231
IivF
ABC transporter related protein
263060
263761
2
702


P1.orf0232

acetyl-CoA acyltransferase
263771
265003
2
1233


P1.orf0233
lcfB
Acyl-CoA synthetase (AMP-forming)/AMP-acid
265013
266563
2
1551




ligase II


P1.orf0234
fadD
AMP-binding enzyme
266573
268081
2
1509


P1.orf0235
SCP2
lipid-transfer protein
268098
269279
3
1182


P1.orf0236

3-oxoacyl-[acyl-carrier protein] reductase
269276
270085
2
810


P1.orf0237

conserved hypothetical protein
270216
270575
3
360


P1.orf0238

F440524_38 putative alcohol dehydrogenase
270819
270637
−1
183


P1.orf0239

conserved hypothetical protein
270867
271124
3
258


P1.orf0240

addiction module toxin, RelE/StbE family
271121
271423
2
303


P1.orf0241

putative Alcohol dehydrogenase, (ADH)
271793
271398
−3
396


P1.orf0242

hypothetical protein
271927
271802
−2
126


P1.orf0243
dhkJ
multi-sensor hybrid histidine kinase
272012
276670
2
4659


P1.orf0244
adhA
Alcohol dehydrogenase GroES domain protein
277765
276710
−2
1056


P1.orf0245
yjfF
inner-membrane translocator
278908
277865
−2
1044


P1.orf0246
ytfT
sugar ABC transporter permease protein
279921
278908
−1
1014


P1.orf0247
ytfR
sugar ABC transporter ATP-binding protein
281503
279995
−2
1509


P1.orf0248
ytfQ
periplasmic binding protein/LacI transcriptional
282545
281586
−3
960




regulator


P1.orf0249
Galm
aldose 1-epimerase
283693
282674
−2
1020


P1.orf0250
yvrE
Smp-30/Cgr1 family protein
284592
283690
−1
903


P1.orf0251
aldH
dehydrogenase
285562
284582
−2
981


P1.orf0252
aldH
NAD-dependent aldehyde dehydrogenase
286131
285559
−1
573


P1.orf0253
gal
putative d-galactose 1-dehydrogenase protein
287110
286163
−2
948


P1.orf0254
ilvD
dihydroxy-acid dehydratase
288839
287097
−3
1743


P1.orf0255

fumarylacetoacetate (FAA) hydrolase
289859
288858
−3
1002


P1.orf0256
xylH
inner membrane permease of D-xylose ABC
291069
289864
−1
1206




transporter


P1.orf0257
araG
ABC transporter related protein
292603
291041
−2
1563


P1.orf0258
chvE
putative sugar uptake ABC transporter
293736
292666
−1
1071




periplasmic solute-binding protein precursor


P1.orf0259
gbpR
transcriptional regulator protein, LysR family
293961
294938
3
978




(possibly activator of the expression of chvE




protein)


P1.orf0260

conserved hypothetical protein
295016
295489
2
474


P1.orf0261

LysR family transcriptional regulator
296609
297565
2
957


P1.orf0262

Alcohol dehydrogenase zinc-binding domain
296536
295508
−2
1029




protein


P1.orf0263

conserved hypothetical protein
297888
297619
−1
270


P1.orf0264

conserved hypothetical protein
298075
297848
−2
228


P1.orf0265

TetR family transcriptional regulator
298803
298174
−1
630


P1.orf0266
mdmC
O-methyltransferase mdmC
298931
299602
2
672


P1.orf0267

major facilitator superfamily transporter
299655
300839
3
1185


P1.orf0268

conserved hypothetical protein
300897
301634
3
738


P1.orf0269
acrF
acriflavin resistance protein
305336
302289
−3
3048


P1.orf0270

efflux transporter, RND family, MFP subunit
306451
305336
−2
1116


P1.orf0271
lmrA
transcriptional regulator
306540
307115
3
576


P1.orf0272
vdlC
short chain dehydrogenase
307162
307977
1
816


P1.orf0273
TRR1
FAD-dependent pyridine nucleotide-disulphide
308915
307995
−3
921




oxidoreductase


P1.orf0274
ywnA
transcriptional regulator, BadM/Rrf2 family
309078
309569
3
492


P1.orf0275

putative effector of murein hydrolase
310317
309583
−1
735


P1.orf0276

hypothetical protein
310717
310310
−2
408


P1.orf0277
pmrA
major facilitator transporter
311875
310790
−2
1086


P1.orf0278
folE
GTP cyclohydrolase I
312630
312004
−1
627


P1.orf0279

conserved hypothetical protein
313395
312655
−1
741


P1.orf0280

conserved hypothetical protein
313853
314236
2
384


P1.orf0281

conserved hypothetical protein
314242
315081
1
840


P1.orf0282

arylmalonate decarboxylase
315223
315966
1
744


P1.orf0283
ydhC
transcriptional regulator, GntR family
315981
316685
3
705


P1.orf0284

putative acyl-CoA transferase/carnitine
316686
317882
3
1197




dehydratase


P1.orf0285
yngG
Hydroxymethylglutaryl-CoA lyase
317894
318838
2
945


P1.orf0286
gbas
conserved hypothetical protein
318835
319149
1
315


P1.orf0287

isochorismatase hydrolase
319182
319835
3
654


P1.orf0288
mauR
LysR family transcriptional regulator
319929
320825
3
897


P1.orf0289
yajO
putative oxidoreductase
321960
320899
−1
1062


P1.orf0290

conserved hypothetical protein
322495
322088
−2
408


P1.orf0291

WD40-like repeat
323353
322676
−2
678


P1.orf0292

conserved hypothetical protein
324680
323472
−3
1209


P1.orf0293

hypothetical protein
325200
325018
−1
183


P1.orf0294
braC
branched chain amino acid ABC transporter
325402
326541
1
1140




periplasmic ligand-binding protein


P1.orf0295
braD
ABC branched chain amino acid family
326563
327480
1
918




transporter, inner membrane subunit


P1.orf0296
braE
High-affinity branched-chain amino acid
327562
328494
1
933




transport system permease protein braE


P1.orf0297
braF
branched-chain amino acid ABC transporter
328491
329318
3
828




ATPase


P1.orf0298
braG
High-affinity branched-chain amino acid
329323
330018
1
696




transport ATP-binding protein braG


P1.orf0299

putative hydrolase
330037
330909
1
873


P1.orf0300
yfdE
L-carnitine dehydratase/bile acid-inducible
331052
332284
2
1233




protein F


P1.orf0301

hypothetical protein
332281
333126
1
846


P1.orf0302
namA
putative FMN oxidoreductase
333123
334307
3
1185


P1.orf0303

MscS mechanosensitive ion channel
335475
334321
−1
1155


P1.orf0304
yagR
aldehyde oxidase and xanthine
337817
335520
−3
2298




dehydrogenase molybdopterin binding


P1.orf0305
yagS
molybdopterin dehydrogenase FAD-binding
338830
337814
−2
1017


P1.orf0306
yagT
oxidoreductase
339306
338830
−1
477


P1.orf0307

hypothetical protein
339700
339341
−2
360


P1.orf0308

conserved hypothetical protein
339834
340160
3
327


P1.orf0309

pyridoxal phosphate biosynthetic protein PdxJ
340527
340315
−1
213


P1.orf0310

cold-shock DNA-binding domain-containing
340993
340799
−2
195




protein


P1.orf0311

DNA ligase D
343739
341301
−3
2439


P1.orf0312

Ku domain-containing protein
344647
343745
−2
903


P1.orf0313

conserved hypothetical protein
344803
345087
1
285


P1.orf0314
mntH
NADPH-dependent FMN reductase
345091
345735
1
645


P1.orf0315
yhdF
Short-chain dehydrogenase/reductase SDR
346645
345794
−2
852


P1.orf0316

hypothetical protein
346993
347313
1
321


P1.orf0317
cmoB
putative SAM-dependent methyltransferase
348091
347378
−2
714


P1.orf0318

topoisomerase IB
349134
348118
−1
1017


P1.orf0319
Uxs1
NAD-dependent epimerase/dehydratase
350294
349191
−3
1104


P1.orf0320

conserved hypothetical protein
351465
350305
−1
1161


P1.orf0321

conserved hypothetical protein
352556
351462
−3
1095


P1.orf0322

conserved hypothetical protein
353707
352553
−2
1155


P1.orf0323

glycosyl transferase, group 1
354876
353704
−1
1173


P1.orf0324

radical SAM domain-containing protein
356162
354864
−3
1299


P1.orf0325
rfbE
NAD-dependent epimerase/dehydratase
358207
356159
−2
2049


P1.orf0326

NAD-dependent epimerase/dehydratase
359322
358204
−1
1119


P1.orf0327
rpoE
ECF subfamily RNA polymerase sigma-24
360114
359527
−1
588




factor


P1.orf0328

conserved hypothetical protein
361269
360760
−1
510


P1.orf0329
cc4
cytochrome c, class I
362430
361306
−1
1125


P1.orf0330

Cytochrome c oxidase caa3-type, assembly
363092
362427
−3
666




factor CtaG-related protein


P1.orf0331

transmembrane prediction
363451
363089
−2
363


P1.orf0332
ctaD
cytochrome c oxidase, subunit I
365985
363466
−1
2520


P1.orf0333
ctaC
cytochrome c oxidase, subunit II
367010
365982
−3
1029


P1.orf0334
gcd
glucose dehydrogenase
369412
367016
−2
2397


P1.orf0335

hypothetical protein
369479
369637
2
159


P1.orf0336

conserved hypothetical protein
370254
370700
3
447


P1.orf0337

conserved hypothetical protein
371128
371805
1
678


P1.orf0338

conserved hypothetical protein
373405
373043
−2
363


P1.orf0339

prevent-host-death family protein
373714
373454
−2
261


P1.orf0340
gabD
Aldehyde Dehydrogenase
375058
373802
−2
1257


P1.orf0341
gsiA
oligopeptide ABC superfamily ATP binding
376993
375308
−2
1686




cassette transporter, ABC protein


P1.orf0342

Putative peptide transport system permease
377814
376990
−1
825




protein


P1.orf0343

binding-protein-dependent transport systems
378769
377825
−2
945




inner membrane component


P1.orf0344

acyl-coenzyme A: 6-aminopenicillanic-acid-
379943
378822
−3
1122




acyltransferase precursor


P1.orf0345

beta-alanine-pyruvate transaminase
381420
380041
−1
1380


P1.orf0346
yjjM
GntR family transcriptional regulator
382417
381500
−2
918


P1.orf0347
bam
Indoleacetamide hydrolase
382501
383991
1
1491


P1.orf0348

poly(aspartic acid) hydrolase
384006
384872
3
867


P1.orf0349

ABC superfamily ATP binding cassette
385008
386591
3
1584




transporter substrate-binding protein


P1.orf0350

transposase, mutator type
387405
387115
−1
291


P1.orf0351

transposase, mutator type
388310
387405
−3
906


P1.orf0352

conserved hypothetical protein
388987
389457
1
471


P1.orf0353

conserved hypothetical protein
390709
389783
−2
927


P1.orf0354

conserved hypothetical protein
391494
390706
−1
789


P1.orf0355

plasmid maintenance system antidote protein,
392861
393004
2
144




XRE family


P1.orf0356

type II restriction enzyme, methylase subunit
393001
393192
1
192


P1.orf0357
hsdR
type I restriction-modification system
393202
396231
1
3030




restriction subunit


P1.orf0358

putative restriction modification system
396266
397510
2
1245




specificity subunit


P1.orf0359
prrC
anticodon nuclease
397507
398736
1
1230


P1.orf0360

type I restriction-modification system, M
398733
400355
3
1623




subunit


P1.orf0361

integrase, catalytic region
400892
401353
2
462


P1.orf0362
xerC
phage integrase family protein
402015
402977
3
963


P1.orf0363

conserved hypothetical protein
403509
402967
−1
543


P1.orf0364

conserved hypothetical protein
403679
405373
2
1695


P1.orf0365

conserved hypothetical protein
405379
407313
1
1935


P1.orf0366

conserved hypothetical protein
407310
408947
3
1638


P1.orf0367

Cold-shock DNA-binding domain protein
408944
411208
2
2265


P1.orf0368

conserved hypothetical protein
411205
412182
1
978


P1.orf0369

conserved hypothetical protein
416011
412664
−2
3348


P1.orf0370
al2
CRISPR-associated protein
416875
418866
1
1992


P1.orf0371

CRISPR-associated protein
418867
419184
1
318


P1.orf0372

selenide, water dikinase
419865
420392
3
528


P1.orf0373
ybfL
transposase, is4 family
420664
421710
1
1047


P1.orf0374
parA
partition protein ParA
422509
423213
1
705


P1.orf0375

hypothetical protein
423411
423569
3
159


P1.orf0376

hypothetical protein
423954
424079
3
126


P1.orf0377
gluA
F213463_1 cellobiase CelA precursor
424429
426300
1
1872


P1.orf0378

maleate cis-trans isomerase protein
427582
426830
−2
753


P1.orf0379
icaR
putative TetR family transcriptional regulator
427725
428483
3
759


P1.orf0380

conserved hypothetical protein
428775
428515
−1
261


P1.orf0381

glyoxalase/bleomycin resistance
429479
428775
−3
705




protein/dioxygenase


P1.orf0382

conserved hypothetical protein
430769
429501
−3
1269


P1.orf0383

conserved hypothetical protein
431298
430777
−1
522


P1.orf0384
vioD
putative monooxygenase, FAD/NAD(P)-
432452
431295
−3
1158




binding domain


P1.orf0385
dhbE
AMP-dependent synthetase and ligase
434172
432484
−1
1689


P1.orf0386
icaR
putative transcriptional regulator
434976
434275
−1
702


P1.orf0387
yiaO
Bacterial extracellular solute-binding protein,
435118
436176
1
1059




family 7


P1.orf0388

conserved hypothetical protein
436161
436919
3
759


P1.orf0389
siaT
TRAP transporter, DctM-like membrane
436879
438048
1
1170




protein


P1.orf0390

putative Aminoglycoside phosphotransferase
439093
438071
−2
1023


P1.orf0391
tam
trans-aconitate methyltransferase
439218
439982
3
765


P1.orf0392
gst
glutathione S-transferase protein
440001
440609
3
609


P1.orf0393
gloA
lactoylglutathione lyase
441036
440632
−1
405


P1.orf0394

conserved hypothetical protein
441639
441223
−1
417


P1.orf0395
acrR
TetR family transcriptional regulator
441721
442362
1
642


P1.orf0396
ifcA
fumarate reductase
443799
442378
−1
1422


P1.orf0397
namA
NADH: flavin oxidoreductase/NADH oxidase
443958
444935
3
978


P1.orf0398
yvoA
transcriptional regulator
445026
445901
3
876


P1.orf0399

branched-chain amino acid transport system
446000
447223
2
1224




substrate-binding protein


P1.orf0400
braD
branched-chain amino acid ABC transporter
447325
448167
1
843




permease protein


P1.orf0401

amino acid ABC transporter permease protein
448167
449150
3
984


P1.orf0402
lptB
ABC transporter ATP-binding protein
449150
449893
2
744


P1.orf0403
IivF
ABC transporter ATP-binding protein
449890
450609
1
720


P1.orf0404

MmgE/PrpD family protein
450622
452040
1
1419


P1.orf0405
prpB
isocitrate lyase family protein
452042
452920
2
879


P1.orf0406

tautomerase
452966
453457
2
492


P1.orf0407
ifcA
putative fumarate reductase/succinate
453463
454821
1
1359




dehydrogenase flavoprotein


P1.orf0408
hyuE
hydantoin racemase
454841
455599
2
759


P1.orf0409
hyuE
hydantoin racemase
455614
456303
1
690


P1.orf0410
lrg1
MmgE/PrpD family protein
456300
457646
3
1347


P1.orf0411

hypothetical protein
457718
457864
2
147


P1.orf0412

hypothetical protein
457895
458059
2
165


P1.orf0413

conserved hypothetical protein
458269
459588
1
1320


P1.orf0414

putative amidase
461101
459668
−2
1434


P1.orf0415
braG
branched chain amino acid ABC transporter
461821
461129
−2
693




ATP-binding protein


P1.orf0416
braF
putative ABC transport system, ATP-bidning
462606
461815
−1
792




protein


P1.orf0417
IivM
putative ABC transport system, membrane
463601
462603
−3
999




protein


P1.orf0418
braD
inner-membrane translocator
464465
463605
−3
861


P1.orf0419

branched-chain amino acid ABC transporter,
465776
464556
−3
1221




periplasmic amino acid-binding protein


P1.orf0420
nanR
regulatory protein GntR HTH
466752
465961
−1
792


P1.orf0421

hypothetical protein
467173
467316
1
144


P1.orf0422

hypothetical protein
467699
467899
2
201


P1.orf0423

hypothetical protein
467955
468107
3
153


P1.orf0424

hypothetical protein
468152
468298
2
147


P1.orf0425

conserved hypothetical protein
468358
469305
1
948


P1.orf0426
algI
membrane bound O-acyl transferase, MBOAT
469322
470677
2
1356


P1.orf0427

hypothetical protein
471709
470678
−2
1032


P1.orf0428

hypothetical protein
472081
471875
−2
207


P1.orf0429

hypothetical protein
472147
472290
1
144


P1.orf0430

hypothetical protein
473393
472362
−3
1032


P1.orf0431

hypothetical protein
473501
473692
2
192


P1.orf0432

conserved hypothetical protein
473712
473978
3
267


P1.orf0433
cinA
competence/damage-inducible protein CinA
473929
474507
1
579


P1.orf0434
cat1
putative acetyl-CoA hydrolase/transferase
474600
476111
3
1512




family protein; putative succinyl-




CoA: coenzyme A transferase


P1.orf0435
alkJ
putative choline dehydrogenase lipoprotein
476491
478116
1
1626




oxidoreductase


P1.orf0436
siaP
TRAP dicarboxylate transporter- DctP subunit
478397
479503
2
1107


P1.orf0437

tripartite ATP-independent periplasmic
479565
480071
3
507




transporter DctQ


P1.orf0438

TRAP dicarboxylate transporter, DctM subunit
480068
481345
2
1278


P1.orf0439
yxeQ
MmgE/PrpD family protein
481377
482729
3
1353


P1.orf0440
maoC
conserved hypothetical protein
482756
483256
2
501


P1.orf0441
mmgC
Butyryl-CoA dehydrogenase
483253
484428
1
1176


P1.orf0442
maoC
conserved hypothetical protein
484439
484921
2
483


P1.orf0443
yfdE
L-carnitine dehydratase/bile acid-inducible
484918
486153
1
1236




protein F


P1.orf0444

Citryl-CoA lyase
486150
487073
3
924


P1.orf0445
kdgR
transcriptional regulator, IclR family
487146
487898
3
753


P1.orf0446

major facilitator transporter
487911
489089
3
1179


P1.orf0447
Ech1
enoyl-CoA hydratase
489947
489102
−3
846


P1.orf0448

hypothetical protein
490444
490049
−2
396


P1.orf0449

hypothetical protein
490967
490515
−3
453


P1.orf0450

hypothetical protein
491546
491067
−3
480


P1.orf0451
gst3
Glutathione S-transferase domain protein
492356
491676
−3
681


P1.orf0452
mmgC
acyl-CoA dehydrogenase
493553
492408
−3
1146


P1.orf0453

Carbamoyl-phosphate synthase L chain ATP-
495592
493598
−2
1995




binding


P1.orf0454

propionyl-CoA carboxylase
497235
495673
−1
1563


P1.orf0455

hypothetical protein
497323
497616
1
294


P1.orf0456
mrcA
glycosyl transferase family protein
498482
497871
−3
612


P1.orf0457

transcriptional regulator
499199
498924
−3
276


P1.orf0458

hypothetical protein
499669
499484
−2
186


P1.orf0459
frpC
bacteriocin
501632
499881
−3
1752


P1.orf0460

hypothetical protein
501804
501619
−1
186


P1.orf0461

hypothetical protein
502315
501893
−2
423


P1.orf0462

putative ENOYL-COA HYDRATASE
502566
503336
3
771


P1.orf0463
araC
transcriptional regulator, AraC family
503355
504209
3
855


P1.orf0464
lcfA
long-chain-fatty-acid--CoA ligase
504260
506095
2
1836


P1.orf0465
rhlG
short-chain dehydrogenase/reductase SDR
506873
506106
−3
768


P1.orf0466
pleC
non-motile and phage-resistance protein
508451
507000
−3
1452


P1.orf0467

membrane protein
508592
508915
2
324


P1.orf0468

conserved hypothetical protein
510548
508890
−3
1659


P1.orf0469

hypothetical protein
510922
510668
−2
255


P1.orf0470
luxQ
sensor histidine kinase
511186
514770
1
3585


P1.orf0471

two component transcriptional regulator
514767
515732
3
966


P1.orf0472

conserved hypothetical protein
515942
516124
2
183


P1.orf0473

hypothetical protein
516354
516656
3
303


P1.orf0474
ynaD
GCN5-related N-acetyltransferase
516833
517366
2
534


P1.orf0475

conserved hypothetical protein
521851
517388
−2
4464


P1.orf0476

conserved hypothetical protein
529416
521851
−1
7566


P1.orf0477

hypothetical protein
530907
529468
−1
1440


P1.orf0478

hemagglutinin protein
534719
530907
−3
3813


P1.orf0479

conserved hypothetical protein
537877
534716
−2
3162


P1.orf0480

conserved hypothetical protein
539720
537975
−3
1746


P1.orf0481

major facilitator transporter
541177
539945
−2
1233


P1.orf0482
arsC
ArsR family transcriptional regulator/protein
541322
542170
2
849




tyrosine phosphatase


P1.orf0483
gap3
glyceraldehyde-3-phosphate dehydrogenase,
542175
543197
3
1023




type I


P1.orf0484

major facilitator transporter
543191
544441
2
1251


P1.orf0485

beta-lactamase
544538
545719
2
1182


P1.orf0486

Cephalosporin hydroxylase
546109
546777
1
669


P1.orf0487

macrocin-O-methyltransferase
546777
547604
3
828


P1.orf0488

conserved hypothetical protein
547960
548265
1
306


P1.orf0489

hypothetical protein
548277
549212
3
936


P1.orf0490

O-linked N-acetylglucosamine transferase
549206
550879
2
1674


P1.orf0491

hypothetical protein
550831
551082
1
252


P1.orf0492

dehydratase, MaoC family protein
551432
551587
2
156


P1.orf0493
citE
HpcH/HpaI aldolase/citrate lyase family
551584
552501
1
918


P1.orf0494

conserved hypothetical protein
552513
553436
3
924


P1.orf0495
IivM
ABC transporter permease protein
555566
554586
−3
981


P1.orf0496
IivH
inner-membrane translocator
556600
555731
−2
870


P1.orf0497
IivF
ABC transporter related protein
557327
556614
−3
714


P1.orf0498
braF
ABC transporter related protein
558115
557324
−2
792


P1.orf0499
IivK
putative leucine/isoleucine/valine-binding
559395
558163
−1
1233




protein precursor


P1.orf0500
baiB
AMP-dependent synthetase and ligase
559600
561189
1
1590


P1.orf0501
mfeB
MaoC-like dehydratase
561274
562128
1
855


P1.orf0502

short-chain dehydrogenase
562184
563104
2
921


P1.orf0503
paaG
crotonase
563120
563935
2
816


P1.orf0504
badR
putative MarR family transcriptional regulator
564533
563985
−3
549


P1.orf0505
fadH
short-chain dehydrogenase/reductase SDR
565439
564561
−3
879


P1.orf0506
ymfI
short-chain dehydrogenase/reductase SDR
566278
565538
−2
741


P1.orf0507

putative acyl-CoA hydratase
567137
566289
−3
849


P1.orf0508
yxbG
2,5-dichloro-2,5-cyclohexadiene-1,4-diol
567916
567161
−2
756




dehydrogenase (2,5-ddol dehydrogenase)


P1.orf0509
menE
long-chain-fatty-acid--CoA ligase
568030
569610
1
1581


P1.orf0510

Acyl-CoA dehydrogenase, middle domain
570905
569721
−3
1185




protein


P1.orf0511

pimeloyl-CoA dehydrogenase
572014
570902
−2
1113


P1.orf0512
lcfB
AMP-dependent synthetase and ligase
573590
572049
−3
1542


P1.orf0513
dctP
putative dicarboxylate-binding periplasmic
573957
575021
3
1065




protein


P1.orf0514

TRAP transporter, DctQ-like membrane
575066
575599
2
534




protein


P1.orf0515

trap dicarboxylate transporter, dctm subunit
575642
576946
2
1305


P1.orf0516

Long-chain specific acyl-CoA dehydrogenase
578341
577181
−2
1161


P1.orf0517

acetyl-CoA acetyltransferase
579540
578338
−1
1203


P1.orf0518

conserved hypothetical protein
581018
579570
−3
1449


P1.orf0519

L-carnitine dehydratase/bile acid-inducible
581258
582325
2
1068




protein F


P1.orf0520
bcd
Acyl-CoA dehydrogenase, C-terminal domain
582357
583523
3
1167




protein


P1.orf0521

Enoyl-CoA hydratase/isomerase
583556
584341
2
786


P1.orf0522
alkR
transcriptional regulator, AraC family protein
585281
584475
−3
807


P1.orf0523

alkyl hydroperoxide reductase/thiol specific
585348
585887
3
540




antioxidant/Mal allergen


P1.orf0524
acsA
acetyl-CoA synthetase/AMP-(fatty) acid ligase
587610
585964
−1
1647




FadDx


P1.orf0525
crt
enoyl-CoA hydratase
587849
588775
2
927


P1.orf0526
OPR3
NADH: flavin oxidoreductase/NADH oxidase
588859
589962
1
1104


P1.orf0527

hypothetical protein
590078
590392
2
315


P1.orf0528
mliC
Membrane-bound lysozyme inhibitor of C-type
590488
590883
1
396




lysozyme


P1.orf0529
csgA
putative short-chain dehydrogenase,
591228
590935
−1
294




oxidoreductase


P1.orf0530
sdh
short-chain dehydrogenase/reductase SDR
591650
591237
−3
414


P1.orf0531

LysR family transcriptional regulator
591776
592696
2
921


P1.orf0532

hexapaptide repeat-containing transferase
592801
593412
1
612


P1.orf0533
xerD
possible integrase-like protein
594709
593429
−2
1281


P1.orf0534
yxjM
putative sensor histidine kinase
595024
597258
1
2235


P1.orf0535
devR
response regulator
597287
598000
2
714


P1.orf0536

conserved hypothetical protein
598984
598019
−2
966


P1.orf0537

Putative TRAP transporter large permease
600291
598999
−1
1293




protein


P1.orf0538

putative DctQ (C4-dicarboxylate permease,
600818
600288
−3
531




small subunit)


P1.orf0539
dctP
C4-dicarboxylate-binding periplasmic protein
601989
600982
−1
1008


P1.orf0540
crt
enoyl-CoA hydratase
602255
603037
2
783


P1.orf0541
tagA
ToxR-activated gene A lipoprotein
603169
605823
1
2655


P1.orf0542
yngI
AMP-dependent synthetase and ligase
607530
605857
−1
1674


P1.orf0543

conserved hypothetical protein
608158
607595
−2
564


P1.orf0544

conserved hypothetical protein
608700
608179
−1
522


P1.orf0545

conserved hypothetical protein
609437
608847
−3
591


P1.orf0546

sulfotransferase
610594
609725
−2
870


P1.orf0547

Hpr(Ser) kinase/phosphatase
611454
610591
−1
864


P1.orf0548

sulfotransferase
611695
612348
1
654


P1.orf0549

putative N-acetylglucosaminyltransferase
614343
613219
−1
1125


P1.orf0550
pcaK
3-hydroxyphenylpropionic acid
615773
614421
−3
1353


P1.orf0551

gentisate 1,2-dioxygenase
616945
615887
−2
1059


P1.orf0552
doxA
rieske (2Fe—2S) domain protein
617264
616950
−3
315


P1.orf0553

aromatic-ring-hydroxylating dioxygenase, beta
617775
617281
−1
495




subunit


P1.orf0554
bphA
aromatic-ring-hydroxylating dioxygenase,
618980
617772
−3
1209




alpha subunit


P1.orf0555
thcD
FAD-dependent pyridine nucleotide-disulphide
619193
620440
2
1248




oxidoreductase


P1.orf0556
iclR
transcriptional regulator
621218
620400
−3
819


P1.orf0557
maiA
maleylacetoacetate isomerase
621321
621962
3
642


P1.orf0558

fumarylacetoacetate hydrolase
622007
622696
2
690


P1.orf0559
mauR
LysR family transcriptional regulator
623860
622940
−2
921


P1.orf0560

methylmalonate-semialdehyde dehydrogenase
624014
625513
2
1500


P1.orf0561

hypothetical protein
625931
628021
2
2091


P1.orf0562

hypothetical protein
628036
628410
1
375


P1.orf0563

peptidase M48, Ste24p
628432
630216
1
1785


P1.orf0564

TRAP transporter solute receptor, TAXI family
630217
631218
1
1002


P1.orf0565

hypothetical protein
631582
631779
1
198


P1.orf0566
badR
MarR family transcriptional regulator
633132
633674
3
543


P1.orf0567
hipO
peptidase M20D, amidohydrolase
633059
631887
−3
1173


P1.orf0568

conserved hypothetical protein
633807
634955
3
1149


P1.orf0569
yogA
putative zinc-binding dehydrogenase
635212
636231
1
1020


P1.orf0570
lcfB
AMP-binding domain protein
636228
638114
3
1887


P1.orf0571

conserved hypothetical protein
638111
638965
2
855


P1.orf0572

TRAP-T family transporter, DctQ (4 TMs)
638962
639525
1
564




subunit


P1.orf0573

TRAP-T family protein transporter, DctM (12
639522
640826
3
1305




TMs) subunit


P1.orf0574

phenylacetic acid degradation-related protein
640842
641276
3
435


P1.orf0575

hypothetical protein
641795
641316
−3
480


P1.orf0576

heme receptor
644452
641879
−2
2574


P1.orf0577
fecR
anti-FecI sigma factor, FecR
645589
644660
−2
930


P1.orf0578

ECF subfamily RNA polymerase sigma-24
646092
645589
−1
504




factor


P1.orf0579

hypothetical protein
646794
646489
−1
306


P1.orf0580

HPr kinase
647747
646791
−3
957


P1.orf0581
asnH
asparagine synthase
649648
647747
−2
1902


P1.orf0582

peptidase S45 penicillin amidase
649895
652276
2
2382


P1.orf0583

enoyl-CoA hydratase
652483
653304
1
822


P1.orf0584
yhgD
transcriptional regulator, TetR family
654155
653328
−3
828


P1.orf0585

conserved hypothetical protein
654646
654152
−2
495


P1.orf0586

hypothetical protein
655893
654871
−1
1023


P1.orf0587
algI
membrane bound O-acyl transferase MBOAT
657349
655880
−2
1470




family protein


P1.orf0588
acpM
putative acyl carrier protein
657482
657718
2
237


P1.orf0589

conserved hypothetical protein
658665
657730
−1
936


P1.orf0590

FkbH like protein
658770
660806
3
2037


P1.orf0591
yafC
transcriptional regulator
661741
660836
−2
906


P1.orf0592

addiction module antitoxin
662033
661743
−3
291


P1.orf0593
dinJ
addiction module antitoxin
662319
662020
−1
300


P1.orf0594

hypothetical protein
662702
662454
−3
249


P1.orf0595

conserved hypothetical protein
662739
666431
3
3693


P1.orf0596

hypothetical protein
666428
667438
2
1011


P1.orf0597

conserved hypothetical protein
667452
671111
3
3660


P1.orf0598

HipA-like
672445
671177
−2
1269


P1.orf0599

XRE family transcriptional regulator
672684
672442
−1
243


P1.orf0600
katE
catalase
675002
672882
−3
2121


P1.orf0601
yhcV
Inosine-5′-monophosphate dehydrogenase
675652
675164
−2
489




related protein


P1.orf0602

phage SPO1 DNA polymerase-related protein
675846
676556
3
711


P1.orf0603
tycC
non-ribosomal peptide synthetase
676765
682599
1
5835


P1.orf0604
tycC
Amino acid adenylation
682572
691364
3
8793


P1.orf0605
lgrC
Amino acid adenylation
691352
692830
2
1479


P2.orf0001
fdh
alcohol dehydrogenase
1
1170
1
1170


P2.orf0002
D
tail protein D
2205
1204
−1
1002


P2.orf0003

phage Tail Protein X
2432
2205
−3
228


P2.orf0004

conserved hypothetical protein
3679
2429
−2
1251


P2.orf0005

hypothetical protein
5381
3663
−3
1719


P2.orf0006

bacteriophage gpE
5834
5529
−3
306


P2.orf0007
FII
similar to probable bacteriophage protein
6419
5916
−3
504


P2.orf0008
FI
phage tail sheath protein
7686
6511
−1
1176


P2.orf0009

hypothetical protein
8166
7690
−1
477


P2.orf0010

conserved hypothetical protein
8989
8177
−2
813


P2.orf0011

conserved hypothetical protein
11125
8993
−2
2133


P2.orf0012

gp15 protein
11987
11139
−3
849


P2.orf0013

phage baseplate J-like protein
12876
11980
−1
897


P2.orf0014

Baseplate assembly protein W
13256
12873
−3
384


P2.orf0015

PAAR
13555
13262
−2
294


P2.orf0016

phage baseplate assembly protein V
14012
13560
−3
453


P2.orf0017

hypothetical protein
14632
14009
−2
624


P2.orf0018

hypothetical protein
15097
14816
−2
282


P2.orf0019
amtB
ammonium transporter
16723
15371
−2
1353


P2.orf0020
tauD
Taurine dioxygenase
17879
17037
−3
843


P2.orf0021

class II aldolase/adducin family protein
18717
17953
−1
765


P2.orf0022

binding-protein-dependent transport systems
19509
18739
−1
771




inner membrane component


P2.orf0023

binding-protein-dependent transport systems
20273
19491
−3
783




inner membrane component


P2.orf0024

ABC transporter related protein
21163
20270
−2
894


P2.orf0025

NMT1/THI5 like domain protein
22221
21181
−1
1041


P2.orf0026

metal-dependent phosphohydrolase
22657
23268
1
612


P2.orf0027
lutR
transcriptional regulatory protein
23401
24090
1
690


P2.orf0028
dhkJ
Signal transduction histidine kinase
24694
29427
1
4734


P2.orf0029
rpfG
two component system, transcriptional
29424
30527
3
1104




regulatory protein


P2.orf0030
ydeR
major facilitator superfamily protein
31739
30543
−3
1197


P2.orf0031
yeaT
transcriptional regulator
31835
32716
2
882


P2.orf0032
yvdB
sulphate transporter
34447
32732
−2
1716


P2.orf0033

XRE family transcriptional regulator
34788
34528
−1
261


P2.orf0034

conserved hypothetical protein
35012
35473
2
462


P2.orf0035
lmrA
transcriptional regulator, TetR family
35497
36090
1
594


P2.orf0036

ABC transporter related protein
36176
38086
2
1911


P2.orf0037

hypothetical protein
38126
38839
2
714


P2.orf0038

ABC superfamily ATP binding cassette
40501
38924
−2
1578




transporter substrate-binding protein


P2.orf0039

poly(aspartic acid) hydrolase
41621
40668
−3
954


P2.orf0040
gsiA
ABC superfamily ATP binding cassette
43229
41646
−3
1584




transporter, ABC protein


P2.orf0041

Dipeptide transport system permease protein
44158
43292
−2
867




dppC


P2.orf0042

Putative peptide transport system permease
45096
44155
−1
942




protein


P2.orf0043

acyl-coenzyme A: 6-aminopenicillanic-acid-
46564
45401
−2
1164




acyltransferase


P2.orf0044
bphR
GntR-family transcriptional regulator
46705
47880
1
1176


P2.orf0045

hypothetical protein
48044
47868
−3
177


P2.orf0046
paiB
Protease synthase and sporulation protein PAI
48505
48155
−2
351


P2.orf0047

amidase family protein
49974
48520
−1
1455


P2.orf0048

secretion protein HlyD family protein
50955
50059
−1
897


P2.orf0049

conserved hypothetical protein
51167
50952
−3
216


P2.orf0050
ydhK
fusaric acid resistance protein region
53257
51164
−2
2094


P2.orf0051
yeaM
AraC family transcriptional regulator
53335
54138
1
804


P2.orf0052

conserved hypothetical protein
54665
55303
2
639


P2.orf0053

conserved hypothetical protein
56402
55323
−3
1080


P2.orf0054

conserved hypothetical protein
56446
57084
1
639


P2.orf0055

conserved hypothetical protein
57088
57966
1
879


P2.orf0056
pheC
extracellular solute-binding protein
58113
58955
3
843


P2.orf0057
glnP
polar amino acid ABC transporter, inner
59028
59690
3
663




membrane subunit


P2.orf0058
tcyC
ABC transporter ATP-binding protein
59687
60472
2
786


P2.orf0059

Chain A, Crystal Structure Of A Muconate
60482
61603
2
1122




Cycloisomerase From




Azorhizobium Caulinodans


P2.orf0060
dadA
FAD dependent oxidoreductase
61661
62932
2
1272


P2.orf0061
ilvB
thiamine pyrophosphate protein central region
62971
64722
1
1752


P2.orf0062
gcvA
LysR family transcriptional regulator
65673
64735
−1
939


P2.orf0063

conserved hypothetical protein
66090
65689
−1
402


P2.orf0064

Beta-lactamase
67350
66328
−1
1023


P2.orf0065

Chloramphenicol acetyltransferase
68054
67404
−3
651


P2.orf0066
yoeA
MATE efflux family protein
69602
68235
−3
1368


P2.orf0067
nccB
RND family efflux transporter MFP subunit
69931
71175
1
1245


P2.orf0068
nolG
acriflavin resistance protein
71172
74234
3
3063


P2.orf0069

GCN5-related N-acetyltransferase
74551
74243
−2
309


P2.orf0070
yagA
ISHne2, transposase
74674
75075
1
402


P2.orf0071

putative periplasmic protein
75622
76551
1
930


P2.orf0072

GCN5-related N-acetyltransferase
78513
78055
−1
459


P2.orf0073

mannitol transporter
78868
79398
1
531


P2.orf0074

DctMS
79403
80728
2
1326


P2.orf0075
dctP
putative extracellular solute-binding protein,
80801
81931
2
1131




family 7


P2.orf0076
menE
citrate synthase
81954
83537
3
1584


P2.orf0077

enoyl CoA hydratase
83534
84376
2
843


P2.orf0078
marA
Multiple antibiotic resistance protein marA
84846
84418
−1
429


P2.orf0079

conserved hypothetical protein
85298
84885
−3
414


P2.orf0080
fct
TonB-dependent siderophore receptor
85472
87691
2
2220


P2.orf0081
fes
esterase
87688
89295
1
1608


P2.orf0082
ytnP
metallo-beta-lactamase
90248
89301
−3
948


P2.orf0083
nahR
Transcriptional regulator, LysR family
90263
91285
2
1023


P2.orf0084
guaA
putative GMP synthase [glutamine-
91310
91993
2
684




hydrolyzing]


P2.orf0085

conserved hypothetical protein
92830
92006
−2
825


P2.orf0086

transcriptional regulator, GntR family
93052
93756
1
705


P2.orf0087
dapA
Dihydrodipicolinate synthase
93875
94837
2
963


P2.orf0088

hypothetical protein
94888
95079
1
192


P2.orf0089
artI
extracellular solute-binding protein
95092
95901
1
810


P2.orf0090
yecS
ABC transporter, membrane spanning protein
95990
96655
2
666




(amino acid)


P2.orf0091
gltK
putative amino acid ABC transporter
96667
97320
1
654




permease protein


P2.orf0092
glnQ
putative amino acid ABC transporter ATP-
97313
98035
2
723




binding protein


P2.orf0093
dadA
D-amino-acid dehydrogenase
98035
99276
1
1242


P2.orf0094

proline racemase
99290
100291
2
1002


P2.orf0095

Tripartite ATP-independent periplasmic
100453
100992
1
540


P2.orf0096
siaT
TRAP dicarboxylate transporter, DctM subunit
100994
102262
2
1269


P2.orf0097

TRAP-type large permease component
102297
103304
3
1008


P2.orf0098

sugar ABC transporter
105174
103435
−1
1740


P2.orf0099

conserved hypothetical protein
105572
105285
−3
288


P2.orf0100
yurM
binding-protein-dependent transport systems
106468
105587
−2
882




inner membrane component


P2.orf0101

sugar ABC transporter
107336
106470
−3
867


P2.orf0102

ABC transporter related protein
108411
107341
−1
1071


P2.orf0103
smoK
ABC transporter related protein
109522
108431
−2
1092


P2.orf0104
glpD
FAD dependent oxidoreductase
111127
109586
−2
1542


P2.orf0105

conserved hypothetical protein
112676
111435
−3
1242


P2.orf0106

hypothetical protein
113264
113103
−3
162


P2.orf0107
cydB
cytochrome d ubiquinol oxidase, subunit II
114258
113248
−1
1011


P2.orf0108
cydA
cytochrome bd ubiquinol oxidase, subunit I
115738
114263
−2
1476


P2.orf0109
lcfB
AMP-dependent synthetase and ligase
116321
117895
2
1575


P2.orf0110

putative extracellular solute-binding protein
117996
119045
3
1050


P2.orf0111

conserved hypothetical protein
119050
119565
1
516


P2.orf0112
siaT
TRAP-type C4-dicarboxylate transport system,
119562
120851
3
1290




large permease component


P2.orf0113
todF
alpha/beta hydrolase fold protein
120931
121773
1
843


P2.orf0114

conserved hypothetical protein
121793
122113
2
321


P2.orf0115
mhpB
protocatechuate 4,5-dioxygenase subunit beta
122110
122937
1
828


P2.orf0116
bedB
CinA3
122951
123274
2
324


P2.orf0117
hcaE
Aromatic-ring-hydroxylating dioxygenase,
123351
124739
3
1389




alpha subunit-like protein


P2.orf0118

3-phenylpropionate dioxygenase subunit beta
124751
125293
2
543


P2.orf0119
bphB
2,3-dihydroxy-2,3-dihydrophenylpropionate
125327
126139
2
813




dehydrogenase


P2.orf0120

2-nitropropane dioxygenase, NPD
126169
127191
1
1023


P2.orf0121
todJ
2-oxopent-4-enoate hydratase
127188
127979
3
792


P2.orf0122

acetaldehyde dehydrogenase
127985
128923
2
939


P2.orf0123

4-hydroxy-2-oxovalerate aldolase
128927
129985
2
1059


P2.orf0124
tetR
regulatory protein TetR
130566
129976
−1
591


P2.orf0125
thcD
ferredoxin--NAD+ reductase
130857
132035
3
1179


P2.orf0126

conserved hypothetical protein
132167
132940
2
774


P2.orf0127
ycdT
diguanylate cyclase
133040
134215
2
1176


P2.orf0128
yhiN
conserved hypothetical protein
135416
134226
−3
1191


P2.orf0129
gacS
multi-sensor hybrid histidine kinase
135604
139797
1
4194


P2.orf0130

isopenicillin N synthase
140642
139803
−3
840


P2.orf0131

hypothetical protein
141315
142607
3
1293


P2.orf0132

hypothetical protein
142771
142646
−2
126


P2.orf0133

transposase, IS4
143023
142853
−2
171


P2.orf0134

transposase
143487
143020
−1
468


P2.orf0135

peptidase M48 Ste24p
145079
144252
−3
828


P2.orf0136
rhtB
RhtB family transporter
145828
145223
−2
606


P2.orf0137
ASN1
putative asparagine synthase (glutamine-
147157
145841
−2
1317




hydrolyzing)


P2.orf0138
ubiE
Generic methyltransferase
148478
147132
−3
1347


P2.orf0139

transcriptional regulator, LysR family
149523
148594
−1
930


P2.orf0140

ATP-binding protein
150384
149590
−1
795


P2.orf0141
dapA
Dihydrodipicolinate synthase
151442
150381
−3
1062


P2.orf0142

conserved hypothetical protein
152393
151569
−3
825


P2.orf0143

conserved hypothetical protein
153423
152380
−1
1044


P2.orf0144

putative ABC transporter permease protein
154241
153420
−3
822


P2.orf0145
mtrR
transcriptional regulatory protein
155062
154388
−2
675


P2.orf0146

Pyrimidine precursor biosynthesis enzyme
155238
156239
3
1002


P2.orf0147

putative transmembrane protein
156889
156278
−2
612


P2.orf0148

transcriptional regulator, TetR family
157618
157037
−2
582


P2.orf0149

Pirin-related protein
157819
158520
1
702


P2.orf0150

dihydroxy-acid dehydratase
158781
160622
3
1842


P2.orf0151

NMT1/THI5-like domain-containing protein
160851
161813
3
963


P2.orf0152

ABC transporter, membrane spanning protein
161910
162695
3
786


P2.orf0153

ABC transporter, nucleotide binding/ATPase
162692
163480
2
789




protein


P2.orf0154
dinJ
addiction module antitoxin
163579
163881
1
303


P2.orf0155

addiction module toxin, RelE/StbE family
163878
164165
3
288


P2.orf0156
ydaP
thiamine pyrophosphate protein domain
165971
164190
−3
1782




protein TPP-binding


P2.orf0157

putative fatty acid beta hydroxylase
166136
167419
2
1284


P2.orf0158

superoxide dismutase protein
167454
167984
3
531


P2.orf0159
yidJ
arylsulfatase A and related enzyme
168829
170619
1
1791


P2.orf0160
pcaC
carboxymuconolactone decarboxylase
171893
170712
−3
1182


P2.orf0161

transcriptional regulator, LysR family
172948
172043
−2
906


P2.orf0162
fdh
zinc-containing alcohol dehydrogenase
173105
174121
2
1017




superfamily protein


P2.orf0163
fabG
short chain dehydrogenase
174920
174168
−3
753


P2.orf0164
ampR
MPR_RHOCA RecName: Full = HTH-type
176285
175293
−3
993




transcriptional activator AmpR emb


P2.orf0165

LAC_RHOCA RecName: Full = Beta-
176386
177288
1
903




lactamase; AltName: Full = Penicillinase; Flags:




Precursor emb


P2.orf0166

TetR family transcriptional regulator
177876
177292
−1
585


P2.orf0167
ykvO
3-oxoacyl-(acyl-carrier-protein) reductase
177978
178733
3
756


P2.orf0168

putative transcriptional regulator, AbrB family
179015
178761
−3
255


P2.orf0169

DNA-binding protein
179385
179083
−1
303


P2.orf0170

conserved hypothetical protein
180464
179562
−3
903


P2.orf0171
mngR
Mannosyl-D-glycerate transport/metabolism
181469
180681
−3
789




system repressor mngR


P2.orf0172
cat2
putative 4-hydroxybutyrate coenzyme A
182704
181442
−2
1263




transferase


P2.orf0173

MmgE/PrpD family protein
182794
184152
1
1359


P2.orf0174
paaK
putative aerobic phenylacetate-CoA ligase
184208
185620
2
1413


P2.orf0175
gatA
glutamyl-tRNA (Gln) amidotransferase, A
185687
187156
2
1470




subunit


P2.orf0176
amiD
Putative amidase
188946
187531
−1
1416


P2.orf0177

NMT1/THI5 like domain protein
189203
190225
2
1023


P2.orf0178

short chain dehydrogenase
190353
191204
3
852


P2.orf0179
hisC
aspartate aminotransferase
191201
192334
2
1134


P2.orf0180
ytxM
abhydrolase 1
192352
193233
1
882


P2.orf0181
allC
allantoate amidohydrolase
193332
194600
3
1269


P2.orf0182
ytbD
major facilitator superfamily MFS_1
195827
194634
−3
1194


P2.orf0183

hypothetical protein
196724
197392
2
669


P2.orf0184

hypothetical protein
197900
197595
−3
306


P2.orf0185
slmA
Transcriptional regulator
198971
198189
−3
783


P2.orf0186

branched-chain amino acid ABC superfamily
200283
199069
−1
1215




ATP binding cassette transporter, binding




protein


P2.orf0187

pyruvate carboxylase
203732
200391
−3
3342


P2.orf0188
caiC
AMP-dependent synthetase and ligase
205403
203736
−3
1668


P2.orf0189
ycdT
diguanylate cyclase
205708
207324
1
1617


P2.orf0190

2-oxoisovalerate dehydrogenase (alpha
207731
208963
2
1233




subunit)


P2.orf0191

2-oxoisovalerate dehydrogenase (beta
208968
209981
3
1014




subunit)


P2.orf0192

branched-chain alpha-keto acid
209985
211337
3
1353




dehydrogenase subunit E2


P2.orf0193
lpdV
dihydrolipoamide dehydrogenase
211342
212739
1
1398


P2.orf0194
iorA
isoquinoline 1-oxidoreductase subunit alpha
213057
213515
3
459


P2.orf0195
iorB
isoquinoline 1-oxidoreductase, beta subunit
213533
215710
2
2178


P2.orf0196
yycB
QbsM like protein
215917
217068
1
1152


P2.orf0197
nemR
TetR family transcriptional regulator
217122
217727
3
606


P2.orf0198
rutB
putative hydrolase
218483
217788
−3
696


P2.orf0199
ytlA
nitrate/sulfonate/taurine/bicarbonate ABC
218671
219723
1
1053




superfamily ATP binding cassette transporter,




binding protein


P2.orf0200
ytlC
taurine ABC superfamily ATP binding cassette
219761
220600
2
840




transporter, ABC protein


P2.orf0201
ytlD
putative ABC transport proteins, inner
220590
221444
3
855




membrane component


P2.orf0202

putative membrane protein, DUF81
221536
222270
1
735


P2.orf0203
gloA
glyoxalase/bleomycin resistance
222718
222281
−2
438




protein/dioxygenase


P2.orf0204

DoxX family protein
223198
222809
−2
390


P2.orf0205

TetR family transcriptional regulator
223870
223322
−2
549


P2.orf0206

alcohol dehydrogenase
223948
224979
1
1032


P2.orf0207

FAD-binding 9 siderophore-interacting domain
225757
224999
−2
759




protein


P2.orf0208
yeaM
transcriptional regulatory protein
226721
225882
−3
840


P2.orf0209
cyaA
ferredoxin
228506
226734
−3
1773


P2.orf0210
nte1
transcriptional regulator, Crp/Fnr family
228613
229329
1
717


P2.orf0211

patatin
230390
229314
−3
1077


P2.orf0212

hypothetical protein
230826
230365
−1
462


P2.orf0213

hypothetical protein
231299
230823
−3
477


P2.orf0214
oruR
PA2556
232461
231394
−1
1068


P2.orf0215
ycfQ
TetR family transcriptional regulator
233166
232561
−1
606


P2.orf0216
yjgI
putative short-chain
233301
234071
3
771




dehydrogenase/oxidoreductase


P2.orf0217

conserved hypothetical protein
235012
234062
−2
951


P2.orf0218
fhuF
ferric iron reductase
235830
235087
−1
744


P2.orf0219
fhuB
transporter
237833
235833
−3
2001


P2.orf0220
fhuC
ABC-type hydroxamate-dependent iron
238609
237830
−2
780




transport system, ATPase component


P2.orf0221
fhuD
periplasmic component, ABC-type
239544
238606
−1
939




hydroxamate-dependent iron transport system


P2.orf0222
fct
TonB-dependent siderophore receptor
241934
239553
−3
2382


P2.orf0223
ompR
two component transcriptional regulator,
242808
242068
−1
741




winged helix family


P2.orf0224

MltA-interacting MipA family protein
243592
242822
−2
771


P2.orf0225
envZ
two component sensor kinase
243801
245078
3
1278


P2.orf0226

putative AraC family transcriptional regulator
246018
245083
−1
936


P2.orf0227

NAD-dependent epimerase/dehydratase
246162
247058
3
897


P2.orf0228
ydbC
putative oxidoreductase
247979
247098
−3
882


P2.orf0229

sensor histidine kinase
251372
248049
−3
3324


P2.orf0230

response regulator receiver protein
252450
251737
−1
714


P2.orf0231

transcriptional regulator/antitoxin, MazE
252681
252908
3
228


P2.orf0232

conserved hypothetical protein
254762
252999
−3
1764


P2.orf0233

transcriptional regulatory protein
255618
254920
−1
699


P2.orf0234
fadK
putative acid-CoA ligase
257373
255661
−1
1713


P2.orf0235
IivF
ABC branched chain amino acid transporter,
258272
257553
−3
720




ATPase subunit


P2.orf0236
IivG
ABC branched chain amino acid transporter,
259047
258265
−1
783




ATPase subunit


P2.orf0237

ABC branched chain amino acid transporter,
260072
259044
−3
1029




inner membrane subunit


P2.orf0238
braD
ABC branched chain amino acid transporter,
260962
260069
−2
894




inner membrane subunit


P2.orf0239

ABC branched chain amino acid transporter,
262424
261135
−3
1290




periplasmic ligandbinding protein


P2.orf0240
lcfB
AMP-dependent synthetase and ligase
264169
262496
−2
1674


P2.orf0241
crt
enoyl-CoA hydratase/isomerase
265037
264222
−3
816


P2.orf0242
yngJ
acyl-CoA dehydrogenase domain-containing
266230
265109
−2
1122




protein


P2.orf0243
IVD
acyl-CoA dehydrogenase domain-containing
267644
266403
−3
1242




protein


P2.orf0244

conserved hypothetical protein
268137
267889
−1
249


P2.orf0245
old
putative ATP-dependent endonuclease of the
268626
270371
3
1746




OLD family protein


P2.orf0246

conserved hypothetical protein
271176
270910
−1
267


P2.orf0247

addiction module antitoxin
270868
270617
−2
252


P2.orf0248

hypothetical protein
271312
271533
1
222


P2.orf0249

short-chain dehydrogenase/reductase SDR
272518
271616
−2
903


P2.orf0250
gylR
IclR family transcriptional regulator
273492
272716
−1
777


P2.orf0251

TRAP-T family transporter, DctM (12 TMs)
274823
273498
−3
1326




subunit


P2.orf0252

hypothetical protein
275340
274813
−1
528


P2.orf0253

conserved hypothetical protein
276464
275328
−3
1137


P2.orf0254

conserved hypothetical protein
276581
277462
2
882


P2.orf0255

aspartate racemase
277466
278194
2
729


P2.orf0256

OsmC family protein
278769
279194
3
426


P2.orf0257

methyl-accepting chemotaxis protein
280931
279228
−3
1704


P2.orf0258

conserved hypothetical protein
281540
281079
−3
462


P2.orf0259
citE
citrate lyase beta chain
282469
281537
−2
933


P2.orf0260
pcaR
transcriptional regulator
283242
282466
−1
777


P2.orf0261

acyltransferase 3
283484
284680
2
1197


P2.orf0262
bam
Indoleacetamide hydrolase
286128
284692
−1
1437


P2.orf0263
paiB
putative regulatory protein
286853
286185
−3
669


P2.orf0264
aes
putative acetyl esterase
287833
286868
−2
966


P2.orf0265
gsiA
putative ABC transporter ATP-binding protein
289497
287845
−1
1653


P2.orf0266
gsiC
binding-protein-dependent transport systems
291379
290399
−2
981




inner membrane component


P2.orf0267
appC
binding-protein-dependent transport systems
290351
289500
−3
852




inner membrane component


P2.orf0268
appA
twin-arginine translocation pathway signal
292974
291382
−1
1593


P2.orf0269
ghrA
putative 2-hydroxyacid dehydrogenase family
294110
293178
−3
933




protein.


P2.orf0270
yeaM
AraC family transcriptional regulator
294233
295117
2
885


P2.orf0271

putative transcriptional regulators containing
295330
295127
−2
204




the CopG/Arc/MetJ DNA-binding domain


P2.orf0272

PRC-barrel domain-containing protein
295879
295508
−2
372


P2.orf0273
etrA
transcriptional regulator, Crp/Fnr family protein
296121
296909
3
789


P2.orf0274

conserved hypothetical protein
297016
298263
1
1248


P2.orf0275

conserved hypothetical protein
298260
298787
3
528


P2.orf0276

DNA ligase III-like protein
298784
299476
2
693


P2.orf0277

HvnC halovibrin
299695
300918
1
1224


P2.orf0278

conserved hypothetical protein
301464
301009
−1
456


P2.orf0279
ybfL
transposase, is4 family
301727
302773
2
1047


P2.orf0280

thioesterase-like protein
303254
302817
−3
438


P2.orf0281

major facilitator superfamily MFS_1
304485
303271
−1
1215


P2.orf0282

conserved hypothetical protein
305396
306409
2
1014


P2.orf0283

conserved hypothetical protein
306406
306660
1
255


P2.orf0284
serS
tRNA synthetase class II (G H P and S)
306703
307707
1
1005


P2.orf0285
yrpB
2-nitropropane dioxygenase NPD
307806
308786
3
981


P2.orf0286
ASMT
O-methyltransferase family 2
308810
309811
2
1002


P2.orf0287

aldo/keto reductase family oxidoreductase
310797
309829
−1
969


P2.orf0288

short-chain dehydrogenase/reductase SDR
311726
310845
−3
882


P2.orf0289
siaT
TRAP transporter, DctM subunit
313045
311756
−2
1290


P2.orf0290

hypothetical protein
313660
313058
−2
603


P2.orf0291
dctP
C4-dicarboxylate-binding periplasmic protein
314806
313769
−2
1038


P2.orf0292

conserved hypothetical protein
317076
314899
−1
2178


P2.orf0293

acyl-CoA dehydrogenase domain-containing
318311
317280
−3
1032




protein


P2.orf0294
IVD1
acyl-CoA dehydrogenase domain protein
319462
318308
−2
1155


P2.orf0295
iclR
Transcriptional regulator kdgR
319747
320523
1
777


P2.orf0296
paaF
putative enoyl-CoA hydratase
320572
321369
1
798


P2.orf0297

transcriptional regulator
321550
321849
1
300


P2.orf0298

plasmid stabilization system protein
321846
322172
3
327


P2.orf0299
lmrB
Lincomycin resistance protein
323465
322224
−3
1242


P2.orf0300

hypothetical protein
326989
323492
−2
3498


P2.orf0301
alsS
acetolactate synthase
327307
328953
1
1647


P2.orf0302
rfbU
glycosyl transferase group 1
330096
329038
−1
1059


P2.orf0303
divJ
histidine kinase response regulator hybrid
331511
330273
−3
1239




protein


P2.orf0304

hypothetical protein
332022
331756
−1
267


P2.orf0305

hypothetical protein
331765
331646
−2
120


P2.orf0306
polC
polymerase epsilon subunit
333137
332193
−3
945


P2.orf0307

conserved hypothetical protein
334008
333151
−1
858


P2.orf0308

putative iron reductase
334199
334945
2
747


P2.orf0309

RNA polymerase ECF-subfamily sigma-70
334903
335475
1
573




factor


P2.orf0310
fecR
transmembrane sensor
335636
336613
2
978


P2.orf0311

acetyl transferase
336764
337423
2
660


P2.orf0312
aatB
aspartate aminotransferase
338676
337426
−1
1251


P2.orf0313

NAD-dependent aldehyde dehydrogenase
340317
338788
−1
1530


P2.orf0314
gcvA
transcriptional regulator
341860
342789
1
930


P2.orf0315

conserved hypothetical protein
341730
340687
−1
1044


P2.orf0316

SpoOM family protein
343262
343699
2
438


P2.orf0317
pcaI
3-oxoacid CoA-transferase, A subunit
343828
344538
1
711


P2.orf0318

hypothetical protein
343269
343153
−1
117


P2.orf0319
pcaJ
3-oxoadipate CoA-transferase
344535
345218
3
684


P2.orf0320
mbtH
MbtH-like protein
345567
345812
3
246


P2.orf0321

putative SyrP-like regulatory protein
346227
346856
3
630


P2.orf0322

putative non-ribosomal peptide synthetase
346919
348349
2
1431


P2.orf0323
lgrC
amino acid adenylation domain protein
348429
367838
3
19410


P2.orf0324
lgrC
PvdI
367789
369903
1
2115


P2.orf0325
lgrD
GRC_BREPA RecName: Full = Linear
369900
381584
3
11685




gramicidin synthase subunit C; Includes:




RecName: Full = ATP-dependent valine




adenylase; Short = ValA; AltName: Full = Valine




activase; Includes: RecName: Full = ATP-




dependent D-valine adenylase; Short = D-ValA;




AltName: Full = D-valine


P2.orf0326
iucB
putative acetylase
381581
382612
2
1032


P2.orf0327
pvdA
L-ornithine 5-monooxygenase
382609
383940
1
1332


P2.orf0328
fhuF
ferric iron reductase protein
383944
384714
1
771


P2.orf0329

hypothetical protein
384753
385127
3
375


P2.orf0330
pvdQ
acyl-homoserine lactone acylase
385517
387064
2
1548


P2.orf0331
fct
ferrichrome-iron transporter
387288
389717
3
2430


P2.orf0332
fhuC
ABC transporter related protein
389797
390591
1
795


P2.orf0333
fhuD
periplasmic binding protein
390613
391512
1
900


P2.orf0334
fhuB
iron-hydroxamate transporter permease
391509
393557
3
2049




subunit


P2.orf0335

pyoverdine ABC export system,
393612
395282
3
1671




permease/ATP-binding protein


P2.orf0336

nitrile hydratase-like protein
395682
395365
−1
318


P2.orf0337

conserved hypothetical protein
396044
395733
−3
312


P2.orf0338

nitrile hydratase
396434
396105
−3
330


P2.orf0339

conserved hypothetical protein
396919
396644
−2
276


P2.orf0340

glutathione-dependent formaldehyde-
397372
397764
1
393




activating GFA


P2.orf0341
aceE
Pyruvate dehydrogenase (acetyl-transferring)
400219
397808
−2
2412


P2.orf0342

transcriptional regulator
400360
400839
1
480


P2.orf0343

conserved hypothetical protein
402074
401058
−3
1017


P2.orf0344
pstB
high-affinity phosphate ABC transporter ATP-
403461
402658
−1
804




binding protein


P2.orf0345
pstA
phosphate ABC transporter permease protein
404321
403458
−3
864


P2.orf0346
pstC
phosphate ABC transporter, inner membrane
405346
404336
−2
1011




subunit PstC


P2.orf0347
pstS
phosphate ABC transporter, periplasmic
406436
405432
−3
1005




phosphate-binding protein


P2.orf0348
PEPM
phosphoenolpyruvate phosphomutase
407744
406830
−3
915


P2.orf0349
aspC
aminotransferase class I and II
408984
407782
−1
1203


P2.orf0350

ABC superfamily ATP binding cassette
410075
409077
−3
999




transporter, binding protein


P2.orf0351
fbpB
inner membrane component of ABC
411775
410132
−2
1644




transporter


P2.orf0352
potA
ABC transporter related protein
412855
411779
−2
1077


P2.orf0353
trpI
transcriptional regulator, LysR family
413003
413887
2
885


P2.orf0354

class II aldolase/adducin family protein
414043
414819
1
777


P2.orf0355
yusQ
4-oxalocrotonate tautomerase
415302
414910
−1
393


P2.orf0356
pecT
LysR family transcriptional regulator
415370
416272
2
903


P2.orf0357
fecR
putative FecR
417310
416303
−2
1008


P2.orf0358
fecI
RNA polymerase sigma-70 family protein
417962
417426
−3
537


P2.orf0359

Sphingosine kinase and enzymes related to
419046
418102
−1
945




eukaryotic diacylglycerol kinase


P2.orf0360

conserved hypothetical protein
421188
419365
−1
1824


P2.orf0361

acyl-CoA dehydrogenase domain protein
421331
422548
2
1218


P2.orf0362

Putative HPr kinase/phosphorylase
422644
423597
1
954


P2.orf0363
siaT
TRAP-T family protein transporter, DctM (12
424975
423671
−2
1305




TMs) subunit


P2.orf0364

conserved hypothetical protein
425520
424972
−1
549


P2.orf0365

Bacterial extracellular solute-binding protein,
426771
425599
−1
1173




family 7


P2.orf0366
lcfB
AMP-dependent synthetase and ligase
428473
426866
−2
1608


P2.orf0367
paaG
enoyl-CoA hydratase/isomerase family protein
429389
428544
−3
846


P2.orf0368

acyl-CoA dehydrogenase-like
430563
429403
−1
1161


P2.orf0369
yurK
GntR family transcriptional regulator
430895
431545
2
651


P2.orf0370
pleC
two-component hybrid sensor and regulator
431729
433306
2
1578


P2.orf0371
degU
transcriptional regulator
433932
433294
−1
639


P2.orf0372
ugpC
lactose transport ATP-binding protein LacK
434169
435335
3
1167


P2.orf0373
yurN
binding-protein-dependent transport systems
435328
436269
1
942




inner membrane component


P2.orf0374
yesQ
binding-protein-dependent transport systems
436266
437108
3
843




inner membrane component


P2.orf0375

sugar binding protein of ABC transporter
437160
438434
3
1275


P2.orf0376
icc
conserved hypothetical protein
438434
439282
2
849


P2.orf0377

conserved hypothetical protein
439858
439541
−2
318


P2.orf0378

conserved hypothetical protein
441210
439870
−1
1341


P2.orf0379
dppD
ABC transporter ATP-binding protein
442939
441290
−2
1650


P2.orf0380

inner membrane component of binding-
443801
442944
−3
858




protein-dependent transport system


P2.orf0381

inner membrane component of binding-
444744
443806
−1
939




protein-dependent transport system


P2.orf0382

extracellular solute-binding protein
446347
444758
−2
1590


P2.orf0383
ybhD
HTH-type transcriptional regulator
446504
447463
2
960


P2.orf0384
acyI
gamma-glutamyltranspeptidase
447601
449319
1
1719


P2.orf0385

putative amidotransferase
449371
450792
1
1422


P2.orf0386

arylmalonate decarboxylase
451589
450840
−3
750


P2.orf0387
citE
HpcH/HpaI aldolase
451750
452613
1
864


P2.orf0388
maoC
MaoC-like dehydratase
452668
453186
1
519


P2.orf0389

conserved hypothetical protein
453403
453849
1
447


P2.orf0390

hypothetical protein
453982
454575
1
594


P2.orf0391

acyl-CoA dehydrogenase domain protein
455977
454583
−2
1395


P2.orf0392
yajO
aldo/keto reductase
456051
457085
3
1035


P2.orf0393

GNAT family acetyltransferase
457600
457082
−2
519


P2.orf0394

nitrile hydratase beta-like protein
458132
457743
−3
390


P2.orf0395
nthA
alpha subunit of nitrile hydratase
458767
458156
−2
612


P2.orf0396

nitrile hydratase beta subunit
459468
458782
−1
687


P2.orf0397

conserved hypothetical protein
460174
459506
−2
669


P2.orf0398
amiC
amino acid ABC transporter
460454
461557
2
1104


P2.orf0399

MarR family transcriptional regulator
461618
462076
2
459


P2.orf0400

conserved hypothetical protein
462391
462104
−2
288


P2.orf0401
acrR
TetR family transcriptional regulator
463014
462388
−1
627


P2.orf0402

Phytoene dehydrogenase and related protein
464630
463011
−3
1620


P2.orf0403

aldehyde dehydrogenase
464849
466288
2
1440


P2.orf0404

Amidase
467917
466403
−2
1515


P2.orf0405
IivF
ABC superfamily ATP binding cassette
468812
468099
−3
714




transporter, ABC protein


P2.orf0406
braF
urea or short-chain amide ABC transporter
469557
468814
−1
744


P2.orf0407

urea or short-chain amide ABC transporter
470626
469559
−2
1068


P2.orf0408
IivH
urea or short-chain amide ABC transporter
471496
470630
−2
867


P2.orf0409
amiC
putative Leu/Ile/Val/Thr/Ala-binding protein
472766
471513
−3
1254


P2.orf0410
amiC
ABC transporter substrate-binding protein
474013
472976
−2
1038


P2.orf0411
yhbI
transcriptional regulatory protein
474233
474652
2
420


P2.orf0412

gamma-aminobutyraldehyde dehydrogenase
476115
474658
−1
1458


P2.orf0413
yybE
putative LysR-family regulatory protein
477038
476112
−3
927


P2.orf0414
puuC
aldehyde dehydrogenase protein
478605
477100
−1
1506


P2.orf0415

conserved hypothetical protein
478912
478616
−2
297


P2.orf0416
adh
alcohol dehydrogenase protein
479205
480242
3
1038


P2.orf0417
sdpR
ArsR family transcriptional regulator
480297
480611
3
315


P2.orf0418

conserved hypothetical protein
480608
480940
2
333


P2.orf0419

ATP-dependent Clp protease proteolytic
480987
481583
3
597




subunit


P2.orf0420
yddV
diguanylate cyclase
481691
483121
2
1431


P2.orf0421
gabD
Aldehyde dehydrogenase (NAD(+))
483197
484633
2
1437


P2.orf0422
bphR
transcriptional regulator protein
485453
484707
−3
747


P2.orf0423
siaT
TRAP dicarboxylate transporter, DctQ subunit
485577
486194
3
618


P2.orf0424
yiaN
TRAP transporter, DctM subunit subfamily
486191
487462
2
1272


P2.orf0425

putative hydroxlacyl-CoA dehydrogenase
487462
488415
1
954


P2.orf0426

trap dicarboxylate transporter- dctp subunit
488412
488996
3
585


P2.orf0427

trap dicarboxylate transporter- dctp subunit
489025
489384
1
360


P2.orf0428
catA
oxidoreductase protein
489463
490338
1
876


P2.orf0429
bphC
glyoxalase/bleomycin resistance
490335
491192
3
858




protein/dioxygenase


P2.orf0430

decarboxylase protein
491197
491901
1
705


P2.orf0431
dmpC
2-hydroxymuconic semialdehyde
491907
493361
3
1455




dehydrogenase


P2.orf0432
hpcG
2-oxo-hepta-3-ene-1,7-dioic acid hydratase
493381
494163
1
783


P2.orf0433
hpcH
HpcH/HpaI aldolase
494173
494940
1
768


P2.orf0434

enoyl-CoA hydratase/isomerase family protein
495939
496742
3
804


P2.orf0435

phenylacetic acid degradation operon negative
495806
495021
−3
786




regulatory protein


P2.orf0436
menE
feruloyl-CoA synthetase
496838
498403
2
1566


P2.orf0437
bphR
GntR family transcriptional regulator
499406
498528
−3
879


P2.orf0438

gentisate 1,2-dioxygenase
499618
500733
1
1116


P2.orf0439

5-carboxymethyl-2-hydroxymuconate Delta-
500863
501768
1
906




isomerase


P2.orf0440

extracellular ligand-binding receptor
501956
503143
2
1188


P2.orf0441
braD
ABC superfamily ATP binding cassette
503251
504168
1
918




transporter, membrane protein


P2.orf0442
IivM
ABC superfamily ATP binding cassette
504180
505202
3
1023




transporter, permease protein


P2.orf0443
braF
ABC transporter related protein
505195
505962
1
768


P2.orf0444
IivF
branched-chain amino acid ABC superfamily
505962
506657
3
696




ATP binding cassette transporter, ABC protein


P2.orf0445
NIT4
nitrilase
506670
507617
3
948


P2.orf0446

conserved hypothetical protein
507630
508274
3
645


P2.orf0447

putative esterase/lipase
508271
509167
2
897


P2.orf0448

hypothetical protein
509275
509907
1
633


P2.orf0449

transcriptional activator FtrB
510630
509926
−1
705


P2.orf0450
narK
nitrite transporter
510877
513570
1
2694


P2.orf0451
narG
nitrate reductase, alpha subunit
513598
517338
1
3741


P2.orf0452
narY
nitrate reductase, beta subunit
517335
518873
3
1539


P2.orf0453
narJ
nitrate reductase molybdenum cofactor
518878
519603
1
726




assembly chaperone


P2.orf0454
narI
respiratory nitrate reductase, gamma subunit
519603
520349
3
747


P2.orf0455
nifM
PpiC-type peptidyl-prolyl cis-trans isomerase
520362
521252
3
891


P2.orf0456

Hemerythrin HHE cation binding domain
521300
521854
2
555




subfamily


P2.orf0457
moeA
molybdopterin biosynthesis protein
521851
523119
1
1269


P2.orf0458

molybdenum ABC transporter ATP-binding
523116
523604
3
489




protein


P2.orf0459
moaA
molybdenum cofactor biosynthesis protein A
523641
524687
3
1047


P2.orf0460
mog
molybdopterin binding domain protein
524716
525222
1
507


P2.orf0461

NnrS family protein
525243
526451
3
1209


P2.orf0462

Cellulose synthesis regulatory protein
526976
526461
−3
516


P2.orf0463

diguanylate cyclase
527952
526945
−1
1008


P2.orf0464
aapP
General L-amino acid transport ATP-binding
528809
528063
−3
747




protein


P2.orf0465
yhdY
amino acid ABC transporter permease
531132
528817
−1
2316


P2.orf0466
aapJ
general L-amino acid transport system
532175
531132
−3
1044




substrate-binding protein


P2.orf0467
dapA
dihydrodipicolinate synthase
533301
532393
−1
909


P2.orf0468
yibF
glutathione S-transferase
534025
533423
−2
603


P2.orf0469
dadA
D-amino-acid dehydrogenase
535329
534022
−1
1308


P2.orf0470
ordL
Glycine/D-amino acid oxidase
536660
535332
−3
1329


P2.orf0471
nocR
transcriptional regulator, LysR family
537755
536838
−3
918


P2.orf0472
amiC
urea ABC transporter, urea binding protein
537992
539263
2
1272


P2.orf0473
braD
urea ABC transporter, permease protein UrtB
539469
540398
3
930


P2.orf0474

urea ABC transporter, permease protein UrtC
540403
541587
1
1185


P2.orf0475
braF
urea ABC transporter, ATP-binding protein
541602
542384
3
783




UrtD


P2.orf0476
IivF
putative ABC transporter ATP-binding
542458
543147
1
690




component


P2.orf0477
fmdA
formamidase
543426
544661
3
1236


P2.orf0478
fmdB
FmdB family regulatory protein
544730
545059
2
330


P2.orf0479

conserved hypothetical protein
545144
545584
2
441


P2.orf0480

conserved hypothetical protein
545695
546876
1
1182


P2.orf0481

conserved hypothetical protein
546881
548323
2
1443


P2.orf0482

conserved hypothetical protein
548442
549665
3
1224


P2.orf0483

ABC transporter related protein
549662
551452
2
1791


P2.orf0484

transcriptional regulator, MarR family
551531
552130
2
600


P2.orf0485
dipZ
thiol-disulfide isomerase and thioredoxins
552180
552671
3
492


P2.orf0486
suhR
Patatin
554481
552685
−1
1797


P2.orf0487
gcvA
transcriptional regulator
555545
554538
−3
1008


P2.orf0488

glyoxalase/bleomycin resistance
555678
556124
3
447




protein/dioxygenase superfamily protein


P2.orf0489
cya
calcium binding hemolysin protein
556302
558818
3
2517


P2.orf0490
yodM
phosphoesterase PA-phosphatase related
559494
558808
−1
687




protein


P2.orf0491
dppC
binding-protein-dependent transport systems
560476
559592
−2
885




inner membrane component


P2.orf0492

putative oligopeptide ABC transporter
561429
560476
−1
954




(permease)


P2.orf0493
gsiB
extracellular solute-binding protein family 5
563119
561512
−2
1608


P2.orf0494
ykfD
oligopeptide/dipeptide ABC transporter, ATP-
564228
563212
−1
1017




binding protein-like


P2.orf0495

oligopeptide/dipeptide ABC transporter, ATP-
565220
564225
−3
996




binding protein-like


P2.orf0496
caiC
AMP-dependent synthetase and ligase
566971
565217
−2
1755


P2.orf0497

conserved hypothetical protein
567938
567177
−3
762


P2.orf0498

B3/4 domain protein
568082
568798
2
717


P2.orf0499

hypothetical protein
569114
569269
2
156


P2.orf0500

conserved hypothetical protein
569625
569290
−1
336


P2.orf0501

hypothetical protein
569983
569669
−2
315


P2.orf0502

phytanoyl-CoA dioxygenase (PhyH) family
570940
570065
−2
876




protein


P2.orf0503
oruR
AraC family transcriptional regulator
571040
572080
2
1041


P2.orf0504
glpR
glycerol-3-phosphate regulon repressor
572197
572976
1
780


P2.orf0505
mhpD
fumarylacetoacetate (FAA) hydrolase
573801
572995
−1
807


P2.orf0506
opdE
major facilitator transporter
575026
573917
−2
1110


P2.orf0507

major facilitator superfamily MFS_1
575433
575083
−1
351


P2.orf0508

regulatory protein
575710
576603
1
894


P2.orf0509

transcriptional regulator
577116
576613
−1
504


P2.orf0510

short chain dehydrogenase/reductase family
577227
577991
3
765




oxidoreductase


P2.orf0511

2-hydroxychromene-2-carboxylate isomerase
578006
578614
2
609


P2.orf0512

conserved hypothetical protein
578810
578622
−3
189


P2.orf0513

AraC family transcriptional regulator
579873
578893
−1
981


P2.orf0514

conserved hypothetical protein
580238
579909
−3
330


P2.orf0515
dke1
acetylacetone-cleaving enzyme
580708
580235
−2
474


P2.orf0516
Glo1
Lactoylglutathione lyase
580954
581478
1
525


P2.orf0517

GntR family transcriptional regulator
581586
582263
3
678


P2.orf0518
coaA
pantothenate kinase
582287
583222
2
936


P2.orf0519
alkJ
GMC family oxidoreductase
583334
584968
2
1635


P2.orf0520

LysR family transcriptional regulator
585483
584983
−1
501


P2.orf0521
yhaJ
LysR family transcriptional regulator
585890
585480
−3
411


P2.orf0522

TrpR binding protein WrbA
585995
586621
2
627


P2.orf0523

TPR domain-containing protein
588934
587846
−2
1089


P2.orf0524
caiC
AMP-dependent synthetase and ligase
590679
589033
−1
1647


P2.orf0525

putative monooxygenase
592736
590775
−3
1962


P2.orf0526

Beta-lactamase
593031
594248
3
1218


P2.orf0527

TRAP-T family transporter, DctP (periplasmic
594350
595498
2
1149




binding) subunit


P2.orf0528

TRAP-T family transporter, DctQ (4 TMs)
595572
596105
3
534




subunit


P2.orf0529
siaT
TRAP-T family transporter, DctM (12 TMs)
596102
597409
2
1308




subunit


P2.orf0530

regulatory protein, TetR
597461
598171
2
711


P2.orf0531

putative transcriptional regulators
598216
598461
1
246


P2.orf0532
ybiO
mscS family protein ybiO
600759
598477
−1
2283


P2.orf0533

Beta-lactamase
600931
602205
1
1275


P2.orf0534

putative Peroxiredoxin
602334
602972
3
639


P2.orf0535

conserved hypothetical protein
603028
603453
1
426


P2.orf0536

conserved hypothetical protein
603785
603438
−3
348


P2.orf0537
rob
putative transcriptional regulator protein, AraC
604467
605375
3
909




family


P2.orf0538
yebQ
major facilitator superfamily MFS_1
606706
605393
−2
1314


P2.orf0539
glxA
AraC family transcriptional regulator
608808
607756
−1
1053


P2.orf0540
soxB
sarcosine oxidase beta subunit family protein
608908
610161
1
1254


P2.orf0541
soxD
sarcosine oxidase delta subunit family protein
610172
610471
2
300


P2.orf0542
soxA
sarcosine oxidase alpha subunit family protein
610468
613470
1
3003


P2.orf0543
soxG
sarcosine oxidase, gamma subunit
613489
614040
1
552


P2.orf0544

hypothetical protein
614270
614079
−3
192


P2.orf0545

conserved hypothetical protein
614962
614510
−2
453


P2.orf0546
leuD
3-isopropylmalate dehydratase, small subunit
615722
615069
−3
654


P2.orf0547
leuC
adenosylcobinamide
617121
615727
−1
1395




kinase/adenosylcobinamide-phosphate




guanylyltransferase


P2.orf0548

L-carnitine dehydratase/bile acid-inducible
618356
617118
−3
1239




protein F


P2.orf0549
GCDH
glutaryl-CoA dehydrogenase
619608
618421
−1
1188


P2.orf0550

isochorismatase hydrolase
620279
619656
−3
624


P2.orf0551
hyuA
hydantoin utilization protein A
622363
620231
−2
2133


P2.orf0552

5-oxoprolinase (ATP-hydrolyzing)
624071
622392
−3
1680


P2.orf0553
yvoA
GntR family transcriptional regulator
625002
624157
−1
846


P2.orf0554

Conserved hypothetical protein
626913
625399
−1
1515


P2.orf0555

conserved hypothetical protein
628844
627255
−3
1590


P2.orf0556

glyoxalase/bleomycin resistance
629129
629605
2
477




protein/dioxygenase


P2.orf0557
sdpR
ArsR family transcriptional regulator
629602
629943
1
342


P2.orf0558

activator of HSP90 ATPase 1 family protein
629940
630437
3
498


P2.orf0559
uhpA
LuxR family transcriptional regulator
630987
630421
−1
567


P2.orf0560

acetyl-CoA acetyltransferase
631151
632326
2
1176


P2.orf0561
menE
putative long-chain-fatty-acid CoA ligase
632336
633874
2
1539


P2.orf0562
hutC
transcriptional regulator, GntR family
634557
633829
−1
729


P2.orf0563

conserved hypothetical protein
634942
634649
−2
294


P2.orf0564
cda1
polysaccharide deacetylase
635146
636012
1
867


P2.orf0565

TRAP transporter, transmembrane protein
636092
636685
2
594


P2.orf0566

Putative TRAP transporter large permease
636682
637965
1
1284




protein


P2.orf0567
yiaO
putative exported protein (TRAP-type transport
638073
639077
3
1005




system, periplasmic component)


P2.orf0568
IRG1
MmgE/PrpD
639094
640503
1
1410


P2.orf0569
mmgC
acyl-CoA dehydrogenase
640689
641858
3
1170


P2.orf0570

L-carnitine dehydratase/bile acid-inducible
641855
643117
2
1263




protein F


P2.orf0571
yfdE
L-carnitine dehydratase/bile acid-inducible
643436
644209
2
774




protein F


P2.orf0572
pilJ
putative methyl accepting chemotaxis protein
644419
646494
1
2076


P2.orf0573

conserved hypothetical protein
647362
646472
−2
891


P2.orf0574

peptide deformylase
647567
648151
2
585


P2.orf0575

short chain dehydrogenase
649016
648183
−3
834


P2.orf0576
ycaN
LysR family transcriptional regulator
649114
650016
1
903


P2.orf0577
ycfQ
TetR family transcriptional regulator
650709
650032
−1
678


P2.orf0578
yjgI
short-chain dehydrogenase/reductase SDR
650818
651558
1
741


P2.orf0579

hypothetical protein
652126
652260
1
135


P2.orf0580

putative Ketopantoate reductase PanE/ApbA
652662
652390
−1
273


P2.orf0581
leuA
2-isopropylmalate synthase
654186
652687
−1
1500


P2.orf0582

hypothetical protein
654661
654545
−2
117


P2.orf0583

conserved hypothetical protein
655211
654687
−3
525


P2.orf0584
pleC
Sensor protein
655466
656701
2
1236


P2.orf0585

conserved hypothetical protein
657778
656801
−2
978


P2.orf0586

sporulation initiation inhibitor protein Soj
658949
659989
2
1041


P2.orf0587

chromosome partitioning protein ParB
659989
660891
1
903


P2.orf0588

GCN5-related N-acetyltransferase
661556
661014
−3
543


P2.orf0589
padR
transcriptional regulator
662176
662706
1
531


P2.orf0590

conserved hypothetical protein
663239
662793
−3
447


P2.orf0591
fadH
NADH: flavin oxidoreductase/NADH oxidase
665393
663282
−3
2112


P2.orf0592

triacylglycerol lipase superfamily protein
666816
665485
−1
1332


P2.orf0593
glxA
AraC family transcription regulator
667905
666955
−1
951


P2.orf0594

conserved hypothetical protein
668034
668333
3
300


P2.orf0595

conserved hypothetical protein
668330
668737
2
408


P2.orf0596
yfiR
regulatory protein TetR
669425
668724
−3
702


P2.orf0597

putative acyltransferase
669523
670626
1
1104


P2.orf0598

putative outer membrane autotransporter
672954
670636
−1
2319




barrel


P2.orf0599

hypothetical protein
687767
686256
−3
1512


P2.orf0600

Invasion protein B homolog
688434
687865
−1
570


P2.orf0601

universal stress protein UspA
689603
688716
−3
888


P2.orf0602

conserved hypothetical protein
689981
689637
−3
345


P3.orf0001
istA
Integrase, catalytic region
1
1509
1
1509


P3.orf0002
istB
IstB domain protein ATP-binding protein
1496
2287
2
792


P3.orf0003

CzcC family heavy metal RND efflux outer
3597
2500
−1
1098




membrane protein


P3.orf0004

transposase, IS4 family protein
4330
4806
1
477


P3.orf0005
alsR
regulatory protein BphR
7279
6389
−2
891


P3.orf0006
copK
Copper resistance protein K
7696
7511
−2
186


P3.orf0007
copD
putative copper resistance D transmembrane
8850
7924
−1
927




protein


P3.orf0008
copC
copper resistance protein CopC
9241
8855
−2
387


P3.orf0009
silP
heavy metal translocating P-type ATPase
11634
9382
−1
2253


P3.orf0010

conserved hypothetical protein
12523
12227
−2
297


P3.orf0011
copB
copper resistance protein B
13520
12750
−3
771


P3.orf0012
pcoA
CopA family copper resistance protein
15354
13756
−1
1599


P3.orf0013
cusR
two component response regulator
15899
16603
2
705


P3.orf0014
cusS
sensor histidine kinase
16857
18011
3
1155


P3.orf0015

ArsR family transcriptional regulator
18941
19288
2
348


P3.orf0016
yqcK
lactoylglutathione lyase
19302
19772
3
471


P3.orf0017
arsC
arsenate reductase
19785
20282
3
498


P3.orf0018

putative sodium bile acid symporter family
20293
21378
1
1086




protein


P3.orf0019
arsC
arsenate reductase
21393
21815
3
423


P3.orf0020
ywzG
transcriptional regulator, PadR family
21914
22216
2
303


P3.orf0021

chromate transporter
22213
23442
1
1230


P3.orf0022
yhbS
acetyltransferase, gnat family
23448
23978
3
531


P3.orf0023
yubM
ParB domain protein nuclease
25667
24186
−3
1482


P3.orf0024
bepE
transporter, HAE1 family
26377
25976
−2
402


P3.orf0025
bepF
multidrug efflux RND transporter, membrane
27438
26470
−1
969




fusion protein MexE


P3.orf0026

relaxase
29882
27960
−3
1923


P3.orf0027

conserved hypothetical protein
30123
30749
3
627


P3.orf0028

RES domain superfamily
30762
31394
3
633


P3.orf0029

conserved hypothetical protein
31479
31844
3
366


P3.orf0030

conserved hypothetical protein
33376
31856
−2
1521


P3.orf0031

conserved hypothetical protein
33749
33390
−3
360


P3.orf0032

conserved hypothetical protein
35140
33746
−2
1395


P3.orf0033

conserved hypothetical protein
36100
35150
−2
951


P3.orf0034

conserved hypothetical protein
36489
36097
−1
393


P3.orf0035

DNA repair protein RadC
37202
36708
−3
495


P3.orf0036

dsba oxidoreductase
37806
37378
−1
429


P3.orf0037

conserved hypothetical protein
41061
38170
−1
2892


P3.orf0038

conserved hypothetical protein
41510
41061
−3
450


P3.orf0039

conserved hypothetical protein
42909
41491
−1
1419


P3.orf0040

putative secreted protein
43810
42899
−2
912


P3.orf0041

putative secreted protein
44499
43807
−1
693


P3.orf0042

conserved hypothetical protein
44894
44496
−3
399


P3.orf0043

conserved hypothetical protein
45265
44906
−2
360


P3.orf0044

conserved hypothetical protein
45515
45282
−3
234


P3.orf0045

plasmid conserved hypothetical protein,
45895
45512
−2
384




RAQPRD family


P3.orf0046

excisionase/Xis, DNA-binding
46099
46569
1
471


P3.orf0047

conserved hypothetical protein
46638
47141
3
504


P3.orf0048

AAA ATPase, central region
47159
48073
2
915


P3.orf0049

conserved hypothetical protein
48537
49037
3
501


P3.orf0050

conserved hypothetical protein
49037
49939
2
903


P3.orf0051

F440523_63 hypothetical protein
50110
50703
1
594


P3.orf0052

conserved hypothetical protein
50770
53337
1
2568


P3.orf0053

conserved hypothetical protein
54127
53378
−2
750


P3.orf0054

conserved hypothetical protein
56190
54124
−1
2067


P3.orf0055

conserved hypothetical protein
56638
56321
−2
318


P3.orf0056

lytic transglycosylase, catalytic
57435
56866
−1
570


P3.orf0057

conserved hypothetical protein
58175
57438
−3
738


P3.orf0058

conserved hypothetical protein
58832
58188
−3
645


P3.orf0059

conserved hypothetical protein
59428
58829
−2
600


P3.orf0060
JBP2
helicase-like protein
61846
59567
−2
2280


P3.orf0061

conserved hypothetical protein
62288
61983
−3
306


P3.orf0062

conserved hypothetical protein
62698
62378
−2
321


P3.orf0063

conserved hypothetical protein
63858
62749
−1
1110


P3.orf0064

conserved hypothetical protein
64570
63923
−2
648


P3.orf0065

conserved hypothetical protein
64907
64647
−3
261


P3.orf0066

conserved hypothetical protein
65331
64924
−1
408


P3.orf0067

conserved hypothetical protein
66560
65871
−3
690


P3.orf0068
yubP
conserved hypothetical protein
67482
66655
−1
828


P3.orf0069

conserved hypothetical protein
68533
67628
−2
906


P3.orf0070

conserved hypothetical protein
69128
68844
−3
285


P3.orf0071

conserved hypothetical protein
69696
69436
−1
261


P3.orf0072
tnpA
Transposase Tn3
70407
69766
−1
642


P3.orf0073
merA
mercuric reductase
72470
70782
−3
1689


P3.orf0074
merP
mercuric transport protein periplasmic protein
72786
72481
−1
306


P3.orf0075
merT
putative mercuric transport protein
73149
72799
−1
351


P3.orf0076
merR
MerR family transcriptional regulator
73221
73628
3
408


P3.orf0077

conserved hypothetical protein
74714
73890
−3
825


P3.orf0078

conserved hypothetical protein
75281
75003
−3
279


P3.orf0079

conserved hypothetical protein
76115
75378
−3
738


P3.orf0080

conserved hypothetical protein
76721
76329
−3
393


P3.orf0081

conserved hypothetical protein
77461
77294
−2
168


P3.orf0082
lspA
signal peptidase II
78322
77942
−2
381


P3.orf0083
cadA
heavy metal translocating P-type ATPase
81247
78446
−2
2802


P3.orf0084
zntR
MerR family transcriptional regulator
81450
81848
3
399


P3.orf0085

sterol desaturase-like protein
82512
82303
−1
210


P3.orf0086

cation efflux protein
82924
83559
1
636


P3.orf0087
topB
DNA topoisomerase III
86313
84283
−1
2031


P3.orf0088
ssb
single-stranded DNA-binding protein
87037
86597
−2
441


P3.orf0089

conserved hypothetical protein
87638
87111
−3
528


P3.orf0090

conserved hypothetical protein
88426
87635
−2
792


P3.orf0091

conserved hypothetical protein
89902
88856
−2
1047


P3.orf0092

conserved hypothetical protein
90658
90098
−2
561


P3.orf0093

conserved hypothetical protein
92352
90673
−1
1680


P3.orf0094

conserved hypothetical protein
92614
92345
−2
270


P3.orf0095

cobyrinic acid a,c-diamide synthase
93473
92598
−3
876


P3.orf0096

phage-related protein
93728
93516
−3
213


P3.orf0097

conserved hypothetical protein
94593
93847
−1
747


P3.orf0098

conserved hypothetical protein
95428
95544
1
117


P3.orf0099

hypothetical protein
95955
96197
3
243


P3.orf0100
iap
Alkaline phosphatase isozyme conversion
98224
96218
−2
2007




protein


P3.orf0101

Pyridoxamine 5′-phosphate oxidase-like, FMN-
99403
98414
−2
990




binding


P3.orf0102
trpB
tryptophan synthase subunit beta
100406
100089
−3
318


P3.orf0103
trpB
tryptophan synthase, beta subunit
101314
100406
−2
909


P3.orf0104

conserved hypothetical protein
102640
101558
−2
1083


P3.orf0105

conserved hypothetical protein
103201
102659
−2
543


P3.orf0106
rpoD
RNA polymerase sigma factor
105716
103623
−3
2094


P3.orf0107
dnaG
DNA primase
107778
105883
−1
1896


P3.orf0108

GatB/Yqey domain superfamily
108256
107789
−2
468


P3.orf0109
carA
carbamoyl-phosphate synthase small chain
108790
109962
1
1173


P3.orf0110
carB
Carbamoylphosphate synthase large subunit
109955
113200
2
3246


P3.orf0111
greA
transcription elongation factor GreA
113454
113912
3
459


P3.orf0112

conserved hypothetical protein
115711
113996
−2
1716


P3.orf0113
abrB
membrane protein AbrB duplication
116368
115796
−2
573


P3.orf0114
lrp
transcriptional regulator
117507
117001
−1
507


P3.orf0115

nucleoside-diphosphate-sugar epimerase
117815
118876
2
1062


P3.orf0116
trxB
thioredoxin reductase (NADPH)
118974
119945
3
972


P3.orf0117
yafC
redox-sensitive transcriptional activator
120106
120996
1
891


P3.orf0118

diguanylate cyclase
121882
121061
−2
822


P3.orf0119

conserved hypothetical protein
122308
121898
−2
411


P3.orf0120

conserved hypothetical protein
122847
122305
−1
543


P3.orf0121

Serine phosphatase RsbU, regulator of sigma
124565
122895
−3
1671




subunit


P3.orf0122

amino acid ABC transporter periplasmic
125412
124600
−1
813




protein


P3.orf0123
yedY
Sulfoxide reductase catalytic subunit yedY
127668
126661
−1
1008


P3.orf0124
aatA
aspartate aminotransferase
129076
127874
−2
1203


P3.orf0125
uvrB
excinuclease ABC subunit B
129371
131581
2
2211


P3.orf0126
mdtA
RND family efflux transporter MFP subunit
131809
132912
1
1104


P3.orf0127

AcrB/AcrD/AcrF family protein
132944
136144
2
3201


P3.orf0128
fabG
3-oxoacyl-[acyl-carrier-protein] reductase
136284
137027
3
744


P3.orf0129
folB
dihydroneopterin aldolase family protein
137128
137535
1
408


P3.orf0130
uvrC
Nuclease subunit of the excinuclease complex
137920
140361
1
2442


P3.orf0131
pgsA
Phosphatidylglycerophosphate synthase
140437
140988
1
552


P3.orf0132
moaE
molybdenum cofactor biosynthesis protein E
141003
141761
3
759


P3.orf0133

Calcium-binding EF-hand protein
141891
142391
3
501


P3.orf0134
sigW
RNA polymerase sigma factor
142388
142996
2
609


P3.orf0135

conserved hypothetical protein
142996
143559
1
564


P3.orf0136

conserved hypothetical protein
143556
143957
3
402


P3.orf0137
azoB
NmrA family protein
144927
144049
−1
879


P3.orf0138
yybR
transcriptional regulator
145036
145386
1
351


P3.orf0139
ilvE
branched-chain amino acid aminotransferase
146392
145484
−2
909


P3.orf0140
petP
transcriptional regulator, MarR family
146478
146960
3
483


P3.orf0141
petR
Response regulator receiver: Transcriptional
147017
147763
2
747




regulatory protein, C-terminal


P3.orf0142
envZ
Signal transduction histidine kinase
147784
149145
1
1362


P3.orf0143

hypothetical protein
149529
149149
−1
381


P3.orf0144
yhcK
diguanylate cyclase
150423
149653
−1
771


P3.orf0145
csaA
chaperonin csaA
150861
150505
−1
357


P3.orf0146

magnetic particle membrane specific GTPase
151425
151000
−1
426




P16


P3.orf0147

conserved hypothetical protein
152319
151645
−1
675


P3.orf0148
rpoH
RNA polymerase factor sigma-32
152518
153435
1
918


P3.orf0149
yuxG
oxidoreductase yuxG
155553
153502
−1
2052


P3.orf0150
mtnA
Initiation factor 2B related
156703
155603
−2
1101


P3.orf0151
mtnP
Purine phosphorylase, family 2
157654
156734
−2
921


P3.orf0152

Cytochrome c1, heme protein precursor
158759
157995
−3
765


P3.orf0153
petB
Cytochrome b/b6-like protein
159988
158759
−2
1230


P3.orf0154
petA
ubiquinol-cytochrome c reductase, iron-sulfur
160561
160016
−2
546




subunit


P3.orf0155
yibK
putative aminotransferase/methyltransferase
161030
161545
2
516


P3.orf0156
hemF
coproporphyrinogen III oxidase
161542
162483
1
942


P3.orf0157

hypothetical protein
162813
162550
−1
264


P3.orf0158

major facilitator transporter
163774
162830
−2
945


P3.orf0159

hypothetical protein
163909
164841
1
933


P3.orf0160

polynucleotide adenylyltransferase region
166208
164859
−3
1350


P3.orf0161

hypothetical protein
166528
166259
−2
270


P3.orf0162
nudL
NUDIX hydrolase
167248
166538
−2
711


P3.orf0163

conserved hypothetical protein
167916
167245
−1
672


P3.orf0164
nudF
MutT/nudix family protein
168572
167877
−3
696


P3.orf0165
aidB
putative acyl-CoA dehydrogenase
170347
168665
−2
1683


P3.orf0166

Transcriptional regulator
171179
170394
−3
786


P3.orf0167

hypothetical protein
171425
171742
2
318


P3.orf0168

conserved hypothetical protein
171819
172907
3
1089


P3.orf0169

YcaO-like family
173005
174360
1
1356


P3.orf0170

Putative nitroreductase
174395
175204
2
810


P3.orf0171

hypothetical protein
175281
177491
3
2211


P3.orf0172

conserved hypothetical protein
177491
177958
2
468


P3.orf0173
ywmD
von Willebrand factor type A
179423
178047
−3
1377


P3.orf0174

transcriptional regulator, LuxR
181216
179585
−2
1632




family/hydrolase, alpha/beta fold family


P3.orf0175
yeeA
Putative DNA methyltransferase yeeA
183092
185971
2
2880


P3.orf0176

LuxR family transcriptional regulator
187094
186264
−3
831


P3.orf0177

SpoVT/AbrB domain-containing protein
187255
187404
1
150


P3.orf0178
ispE
4-diphosphocytidyl-2C-methyl-D-erythritol
188600
187665
−3
936




kinase


P3.orf0179

TPR repeat-containing protein
190357
188618
−2
1740


P3.orf0180

conserved hypothetical protein
190896
191087
3
192


P3.orf0181

hypothetical protein
193001
191250
−3
1752


P3.orf0182

hypothetical protein
193258
193461
1
204


P3.orf0183

peptidase M24
195602
193539
−3
2064


P3.orf0184
prmA
ribosomal protein L11 methylase
196575
195634
−1
942


P3.orf0185
uvrD
UvrD/REP helicase domain protein
198952
196559
−2
2394


P3.orf0186

conserved hypothetical protein
200143
199112
−2
1032


P3.orf0187
asnO
Asparagine synthase (glutamine-hydrolyzing)
202308
200392
−1
1917


P3.orf0188
yeaM
pyridoxamine 5′-phosphate oxidase-related
203165
202365
−3
801




protein


P3.orf0189

pyridoxamine 5′-phosphate oxidase-related
203259
203870
3
612




FMN-binding


P3.orf0190

conserved hypothetical protein
203949
204098
3
150


P3.orf0191
yfkN
5′-nucleotidase
205696
204164
−2
1533


P3.orf0192

pentapeptide repeat-containing protein
206223
205687
−1
537


P3.orf0193

D-lactate dehydrogenase
206420
207871
2
1452


P3.orf0194
ssuA
Putative aliphatic sulfonates-binding protein
208092
209174
3
1083


P3.orf0195

ABC transporter ATP-binding protein
209185
209952
1
768


P3.orf0196
tauC
binding-protein-dependent transport systems
209945
210766
2
822




inner membrane component


P3.orf0197
zntB
Mg2+ transporter protein, CorA-like
210794
211834
2
1041


P3.orf0198

transcriptional regulator, GntR family with
214073
212691
−3
1383




aminotransferase domain


P3.orf0199

conserved hypothetical protein
214491
214973
3
483


P3.orf0200
nitA
putative amidohydrolase
215030
216106
2
1077


P3.orf0201

Radical SAM domain protein
216189
217202
3
1014


P3.orf0202

putative histone acetyltransferase
217199
217756
2
558


P3.orf0203

selenophosphate synthetase-related protein
217753
218772
1
1020


P3.orf0204

glycosyl transferase group 1
218769
219959
3
1191


P3.orf0205

conserved hypothetical protein
219956
220243
2
288


P3.orf0206

putative flavoprotein involved in K+ transport
220249
221529
1
1281


P3.orf0207

conserved hypothetical protein
221858
221541
−3
318


P3.orf0208

putative oxidoreductase protein
222622
221873
−2
750


P3.orf0209
ycaN
transcriptional regulator, LysR family
223647
222733
−1
915


P3.orf0210

enoyl-CoA hydratase
223743
224489
3
747


P3.orf0211
Hadh
3-hydroxyacyl-CoA dehydrogenase
224636
225592
2
957


P3.orf0212
ybfI
transcriptional activator
226298
226915
2
618


P3.orf0213
pamO
steroid monooxygenase
227173
228822
1
1650


P3.orf0214
metX
esterase
228849
229904
3
1056


P3.orf0215

conserved hypothetical protein
230906
230031
−3
876


P3.orf0216

TetR family transcriptional regulator
231603
230980
−1
624


P3.orf0217
ybhR
multidrug ABC transporter permease
232801
231680
−2
1122




component


P3.orf0218
ybhS
multidrug ABC transporter permease
233368
232805
−2
564




component


P3.orf0219
ybhS
putative ABC transporter permease protein
233931
233365
−1
567


P3.orf0220
ybhF
ABC transporter related protein
235682
233928
−3
1755


P3.orf0221

membrane protein
236706
235687
−1
1020


P3.orf0222
ybiH
TetR family transcriptional regulator
237326
236703
−3
624


P3.orf0223

conserved hypothetical protein
238615
237500
−2
1116


P3.orf0224
paaA
phenylacetic acid degradation protein (similar
239551
238799
−2
753




to paaA)


P3.orf0225

hypothetical protein
240236
239757
−3
480


P3.orf0226

Radical SAM domain protein
241498
240233
−2
1266


P3.orf0227
hcaE
Rieske (2Fe—2S) domain-containing protein
242879
241698
−3
1182


P3.orf0228

thioesterase superfamily protein
243659
243189
−3
471


P3.orf0229
ydeM
MaoC-like dehydratase
244141
243656
−2
486


P3.orf0230
ligA
NAD-dependent DNA ligase
246344
244233
−3
2112


P3.orf0231
recN
DNA repair protein RecN
248114
246438
−3
1677


P3.orf0232

DNA uptake lipoprotein
249069
248191
−1
879


P3.orf0233

DNA-binding protein
249926
250372
2
447


P3.orf0234
lpxC
UDP-3-O-[3-hydroxymyristoyl] N-
251433
250468
−1
966




acetylglucosamine deacetylase


P3.orf0235
ftsZ
cell division protein FtsZ
253351
251786
−2
1566


P3.orf0236
ftsA
F492457_3 cell division protein FtsA
254667
253432
−1
1236


P3.orf0237
ftsQ
Cell division protein FtsQ
255726
254782
−1
945


P3.orf0238
ddl
D-alanine-D--alanine ligase
256628
255714
−3
915


P3.orf0239
murB
UDP-N-acetylenolpyruvoylglucosamine
257635
256625
−2
1011




reductase


P3.orf0240
murC
UDP-N-acetylmuramate--L-alanine ligase
259059
257635
−1
1425


P3.orf0241
murG
UDP-N-acetylglucosamine-N-acetylmuramyl-
260279
259056
−3
1224




(pentapeptide) pyrophosphoryl-undecaprenol




N-acetylglucosamine transferase


P3.orf0242
ftsW
putative cell division protein ftsW
261397
260276
−2
1122


P3.orf0243
murD
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
262860
261394
−1
1467




synthetase


P3.orf0244
mraY
Glycosyl transferase, family 4
263966
262878
−3
1089


P3.orf0245
murF
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
265477
264020
−2
1458




alanine ligase subfamily


P3.orf0246
murE
UDP-N-acetylmuramoylalanyl-D-glutamate--
266946
265474
−1
1473




2,6-diaminopimelate ligase


P3.orf0247
penA
peptidoglycan synthetase FtsI
268828
266933
−2
1896


P3.orf0248

periplasmic protein
269292
268825
−1
468


P3.orf0249
mraW
S-adenosyl-methyltransferase mraW
270293
269289
−3
1005


P3.orf0250
mraZ
cell division protein MraZ
270811
270290
−2
522


P3.orf0251
ampD
negative regulator of AmpC, AmpD
272679
271888
−1
792


P3.orf0252
eamA
integral membrane protein
273634
272702
−2
933


P3.orf0253
hfaC
ABC transporter related protein
275499
273631
−1
1869


P3.orf0254

curculin domain-containing protein
276723
275686
−1
1038


P3.orf0255
djlA
DnaJ-like protein DjlA
277594
276848
−2
747


P3.orf0256

bacterial extracellular solute-binding protein,
277977
278957
3
981




family 7


P3.orf0257

tripartite ATP-independent periplasmic
279099
279656
3
558




transporter, DctQ component


P3.orf0258

TRAP dicarboxylate transporter, DctM subunit
279676
280986
1
1311


P3.orf0259
OXP1
5-oxoprolinase (ATP-hydrolyzing)
281063
284704
2
3642


P3.orf0260

conserved hypothetical protein
284715
285302
3
588


P3.orf0261

conserved hypothetical protein
285447
285947
3
501


P3.orf0262

conserved hypothetical protein
285944
287074
2
1131


P3.orf0263

conserved hypothetical protein
287088
287303
3
216


P3.orf0264
yneJ
putative transcriptional regulator protein, LysR
287445
288308
3
864




family


P3.orf0265

putative transmembrane protein
288326
289105
2
780


P3.orf0266

pyruvate, phosphate dikinase
289409
291367
2
1959


P3.orf0267
ydcN
XRE family transcriptional regulator
292040
291459
−3
582


P3.orf0268

Gluconate 2-dehydrogenase subunit 3
292191
292955
3
765


P3.orf0269

Gluconate 2-dehydrogenase (acceptor)
293016
294779
3
1764


P3.orf0270

diheme cytochrome c SoxE
294776
295147
2
372


P3.orf0271
ykpB
Ketopantoate reductase
295209
296135
3
927


P3.orf0272
ydeK
transporter ydeK
296132
296998
2
867


P3.orf0273
hxlR
transcriptional regulator
297307
297005
−2
303


P3.orf0274
gstB
glutathione S-transferase
297503
298117
2
615


P3.orf0275

conserved hypothetical protein
298114
298491
1
378


P3.orf0276

4-oxalocrotonate tautomerase family protein
298504
298710
1
207


P3.orf0277
thcD
FAD-dependent pyridine nucleotide-disulphide
300150
298870
−1
1281




oxidoreductase


P3.orf0278
hrb
rubredoxin reductase
300460
300251
−2
210


P3.orf0279

conserved hypothetical protein
301498
300509
−2
990


P3.orf0280
glyA
glycine hydroxymethyltransferase
303294
302002
−1
1293


P3.orf0281
yfeJ
glutamine amidotransferase class-I
304426
303725
−2
702


P3.orf0282
ptsJ
GntR family transcriptional regulator
304715
306028
2
1314


P3.orf0283

cupin 2 domain-containing protein
306617
306231
−3
387


P3.orf0284
yedZ
Ferric reductase domain protein protein
306862
307848
1
987




transmembrane component domain protein


P3.orf0285
ydeR
Major facilitator superfamily MFS_1
308978
307812
−3
1167


P3.orf0286
ycaN
LysR family transcriptional regulator
310241
311167
2
927


P3.orf0287
yajO
aldo/keto reductase
310081
309035
−2
1047


P3.orf0288
menE
citrate synthase
311990
313657
2
1668


P3.orf0289

putative glutathione S-transferase-related
313654
314640
1
987




protein


P3.orf0290

short-chain dehydrogenase/reductase SDR
314637
315410
3
774


P3.orf0291
creA
CreA family protein
315498
315989
3
492


P3.orf0292

LemA family protein
316110
316691
3
582


P3.orf0293
htpX
HtpX-2 peptidase
316696
317859
1
1164


P3.orf0294
virR
conserved hypothetical protein
318375
317875
−1
501


P3.orf0295
fixR
short-chain dehydrogenase
318540
319295
3
756


P3.orf0296

hypothetical protein
320005
320121
1
117


P3.orf0297
yjfL
inner membrane protein YjfL
320245
320667
1
423


P3.orf0298

conserved hypothetical protein
320745
321326
3
582


P3.orf0299
ygiC
glutathionylspermidine synthase
321341
322552
2
1212


P3.orf0300

conserved hypothetical protein
323212
322577
−2
636


P3.orf0301
cqsS
CAI-1 autoinducer sensor kinase/phosphatase
324242
323349
−3
894




cqsS


P3.orf0302
pleC
response regulator receiver sensor signal
325762
324248
−2
1515




transduction histidine kinase


P3.orf0303
iorA
indolepyruvate ferredoxin oxidoreductase,
329497
325985
−2
3513




alpha subunit


P3.orf0304

putative TetR family protein receptor protein
330225
329839
−1
387


P3.orf0305

conserved hypothetical protein
330266
330535
2
270


P3.orf0306

conserved hypothetical protein
330489
330956
3
468


P3.orf0307
zraR
two component, sigma54 specific, Fis family
332800
331025
−2
1776




transcriptional regulator


P3.orf0308
yjbG
Oligoendopeptidase F homolog
332996
334807
2
1812


P3.orf0309

aarF domain-containing kinase
334862
336265
2
1404


P3.orf0310

hypothetical protein
336546
337256
3
711


P3.orf0311

diguanylate cyclase/phosphodiesterase
339938
337284
−3
2655


P3.orf0312
hipO
amidohydrolase
341077
339941
−2
1137


P3.orf0313

hypothetical protein
341375
341542
2
168


P3.orf0314
ilvG
thiamine pyrophosphate protein central region
341626
343383
1
1758


P3.orf0315

conserved hypothetical protein
343422
345497
3
2076


P3.orf0316

conserved hypothetical protein
345494
347092
2
1599


P3.orf0317

conserved hypothetical protein
347500
347114
−2
387


P3.orf0318

conserved hypothetical protein
347766
347518
−1
249


P3.orf0319
ttuD
hydroxypyruvate reductase
349150
347876
−2
1275


P3.orf0320

Creatininase
349350
350093
3
744


P3.orf0321

N-formylglutamate amidohydrolase
350106
350867
3
762


P3.orf0322

conserved hypothetical protein
350920
351222
1
303


P3.orf0323
ytcJ
amidohydrolase 3
353140
351260
−2
1881


P3.orf0324

conserved hypothetical protein
353483
353193
−3
291


P3.orf0325
ybfI
transcriptional regulator, AraC family
353634
354530
3
897


P3.orf0326

conserved hypothetical protein
354659
354910
2
252


P3.orf0327

conserved hypothetical protein
355041
355385
3
345


P3.orf0328

hypothetical protein
355851
355375
−1
477


P3.orf0329

conserved hypothetical protein
356637
355870
−1
768


P3.orf0330

conserved hypothetical protein
357494
356634
−3
861


P3.orf0331

conserved hypothetical protein
357888
357541
−1
348


P3.orf0332
cqsA
CAI-1 autoinducer synthase
358328
359608
2
1281


P3.orf0333
SRY1
Threo-3-hydroxyaspartate ammonia-lyase
360652
359672
−2
981


P3.orf0334
ydfD
transcriptional regulator, GntR family with
360821
362302
2
1482




aminotransferase domain


P3.orf0335

L-lactate permease
362445
364148
3
1704


P3.orf0336

transcriptional regulator
364222
364560
1
339


P3.orf0337
arsB
arsenical-resistance protein
364569
365630
3
1062


P3.orf0338

NADPH-dependent FMN reductase
365627
366391
2
765


P3.orf0339
ggt
gamma-glutamyltranspeptidase
366507
368312
3
1806


P3.orf0340

epoxide hydrolase protein
368348
369241
2
894


P3.orf0341

AraC family transcriptional regulator
369241
370308
1
1068


P3.orf0342

putative regulator PrlF
370305
370499
3
195


P3.orf0343

conserved hypothetical protein
371729
370581
−3
1149


P3.orf0344

conserved hypothetical protein
372148
371726
−2
423


P3.orf0345

conserved hypothetical protein
372478
373455
1
978


P3.orf0346
ddpA
ABC transporter substrate-binding protein
373624
375249
1
1626


P3.orf0347
dppB
ABC transporter permease protein 2
375326
376348
2
1023


P3.orf0348
ddpC
ABC transporter permease protein 1
376345
377193
1
849


P3.orf0349
gsiA
ABC transporter ATP-binding protein
377190
378911
3
1722


P3.orf0350
mcbR
Transcriptional Regulator, GntR family protein
378980
379708
2
729


P3.orf0351

aminotransferase class-III
379990
381345
1
1356


P3.orf0352

hydantoin utilization protein
381360
383396
3
2037


P3.orf0353

putative N-methylhydantoinase B
383401
385014
1
1614


P3.orf0354
ygaY
major facilitator transporter
386318
385119
−3
1200


P3.orf0355

HxlR family transcriptional regulator
386421
386861
3
441


P3.orf0356

GntR family transcriptional regulator
387573
386902
−1
672


P3.orf0357
bcpA
isocitrate lyase and phosphorylmutase
387704
388567
2
864


P3.orf0358

isopropylmalate isomerase large subunit
388564
389985
1
1422


P3.orf0359
leuD
isopropylmalate isomerase small subunit
389985
390620
3
636


P3.orf0360
yraM
conserved hypothetical protein
390701
391888
2
1188


P3.orf0361
dctP
c4-dicarboxylate-binding periplasmic protein
391939
392961
1
1023


P3.orf0362

TRAP-type C4-dicarboxylate transport system
392966
393532
2
567




small permease


P3.orf0363

C4-dicarboxylate TRAP-T family tripartite ATP-
393529
394812
1
1284




independent periplasmic transporter,




membrane protein, large subunit


P3.orf0364
citH
proline/glycine betaine transporter, Major
394995
396287
3
1293




facilitator superfamily


P3.orf0365
mqsR
Motility quorum-sensing regulator mqsR
396479
396775
2
297


P3.orf0366
ygiT
transcriptional regulator, XRE family
396777
397178
3
402


P3.orf0367

hypothetical protein
397787
398224
2
438


P3.orf0368
lcfA
long-chain-fatty-acid--CoA ligase
400068
398320
−1
1749


P3.orf0369
ygfF
short-chain dehydrogenase/reductase SDR
400154
400912
2
759


P3.orf0370
bioC
methyltransferase
400934
401659
2
726


P3.orf0371

F377340_3 AraC
402662
401670
−3
993


P3.orf0372

dienelactone hydrolase family protein
402712
403524
1
813


P3.orf0373

conserved hypothetical protein
403624
404664
1
1041


P3.orf0374
pleC
sensory transduction histidine kinase
406997
404673
−3
2325


P3.orf0375
dht
dihydropyrimidinase
408628
407168
−2
1461


P3.orf0376
pucI
cytosine/purines, uracil, thiamine, allantoin
410219
408690
−3
1530




transporter


P3.orf0377
yeiA
dihydroorotate dehydrogenase family protein
411740
410436
−3
1305


P3.orf0378
yeiT
putative oxidoreductase
413141
411768
−3
1374


P3.orf0379
hyuC
amidase, hydantoinase/carbamoylase family
414563
413307
−3
1257


P3.orf0380
mmsA
methylmalonate-semialdehyde dehydrogenase
416162
414663
−3
1500


P3.orf0381
rutR
transcriptional regulator, TetR family protein
416868
417575
3
708


P3.orf0382
suhB
putative inositol monophosphatase
417572
418438
2
867


P3.orf0383

CRISPR-associated protein Cas2
418894
418604
−2
291


P3.orf0384

conserved hypothetical protein
420031
418940
−2
1092


P3.orf0385

putative crispr-associated protein cas4
420690
420028
−1
663


P3.orf0386

CRISPR-associated Csh2 family protein
421602
420730
−1
873


P3.orf0387

CRISPR-associated protein, Csd1 family
423567
421660
−1
1908


P3.orf0388

CRISPR-associated protein Cas5 family
424163
423564
−3
600


P3.orf0389

helicase
426840
424429
−1
2412


P3.orf0390

conserved hypothetical protein
427477
427037
−2
441


P3.orf0391

Pirin domain protein
428530
427601
−2
930


P3.orf0392
ybfI
transcriptional regulator, AraC family
429531
428647
−1
885


P3.orf0393
mdeA
Cystathionine gamma-synthase
429596
430801
2
1206


P3.orf0394
ydeL
transcriptional regulator, GntR family with
432223
430811
−2
1413




aminotransferase domain protein


P3.orf0395

FMN-binding negative transcriptional regulator
432301
432936
1
636


P3.orf0396

putative nitronate monooxygenase
434092
433112
−2
981


P3.orf0397

HxlR family transcriptional regulator
434310
434867
3
558


P3.orf0398

conserved hypothetical protein
435492
434926
−1
567


P3.orf0399

D-isomer specific 2-hydroxyacid
435713
436675
2
963




dehydrogenase NAD-binding


P3.orf0400

hypothetical protein
438321
436699
−1
1623


P3.orf0401

2OG-Fe(II) oxygenase family protein
438775
439785
1
1011


P3.orf0402

hypothetical protein
438564
438355
−1
210


P3.orf0403

Glyoxalase/bleomycin resistance
439832
440221
2
390




protein/dioxygenase


P3.orf0404
med
Bmp family membrane protein
440300
441394
2
1095


P3.orf0405
yufO
ABC transporter related protein
441436
442977
1
1542


P3.orf0406
yufP
inner-membrane translocator
442970
444100
2
1131


P3.orf0407
yufQ
branched chain amino acid ABC transporter,
444097
445029
1
933




permease protein


P3.orf0408
amn
AMP nucleosidase
445120
446628
1
1509


P3.orf0409

hypothetical protein
446711
447028
2
318


P3.orf0410

HicB family protein
447193
447432
1
240


P3.orf0411

conserved hypothetical protein
447502
448788
1
1287


P3.orf0412

SupD
448785
449744
3
960


P3.orf0413
yakc
aldo/keto reductase
449848
450882
1
1035


P3.orf0414
yhgD
TetR family transcriptional regulator
450910
451506
1
597


P3.orf0415

AraC family transcriptional regulator
451595
452563
2
969


P3.orf0416

major facilitator transporter
452652
453845
3
1194


P3.orf0417
fyuA
TonB-dependent receptor
453931
456051
1
2121


P3.orf0418

O-methyltransferase family protein
456051
457061
3
1011


P3.orf0419
yafC
transcriptional regulator protein
457981
457088
−2
894


P3.orf0420
stp
transporter protein
458114
459625
2
1512


P3.orf0421
NQO1
NAD(P)H quinone oxidoreductase
460322
459654
−3
669


P3.orf0422
nahR
LysR substrate binding domain protein
460421
461374
2
954


P3.orf0423
yebE
Inner membrane protein yebE
462176
461334
−3
843


P3.orf0424

acetyl-CoA carboxylase
462381
463019
3
639


P3.orf0425
yjbB
Na/Pi-cotransporter II-related protein
464741
463029
−3
1713


P3.orf0426

hypothetical protein
465315
464797
−1
519


P3.orf0427

hypothetical protein
465715
465299
−2
417


P3.orf0428

NADH-ubiquinone oxidoreductase
466049
465705
−3
345


P3.orf0429
PED1
Acetyl-CoA C-acyltransferase
467453
466269
−3
1185


P3.orf0430
braG
putative high-affinity branched-chain amino
468382
467660
−2
723




acid transport ATP-binding proteinputative


P3.orf0431
braF
ABC superfamily ATP binding cassette
469152
468379
−1
774




transporter, ABC protein


P3.orf0432

branched-chain amino acid ABC transporter
470993
469149
−3
1845


P3.orf0433

putative branched-chain amino acid ABC
472322
471105
−3
1218




transporter, periplasmic substrate-binding




protein


P3.orf0434

dehydrogenase
473394
472480
−1
915


P3.orf0435
rsmA
Methyltransferase type 12
474041
473445
−3
597


P3.orf0436
iorB
Aldehyde oxidase and xanthine
476400
474082
−1
2319




dehydrogenase, molybdopterin binding protein


P3.orf0437
iorA
Membrane-bound aldehyde dehydrogenase
476874
476410
−1
465




iron-sulfur protein


P3.orf0438
yqhC
transcriptional regulator protein
477114
478010
3
897


P3.orf0439
apl
putative alkaline phosphatase protein
478111
478704
1
594


P3.orf0440
sbcD
nuclease SbcCD, D subunit
478813
480060
1
1248


P3.orf0441
sbcC
exonuclease SbcC
480060
483848
3
3789


P3.orf0442

aminotransferase
485304
483934
−1
1371


P3.orf0443
ydeL
transcriptional regulator, GntR family
485526
486989
3
1464


P3.orf0444
mocR
transcriptional regulator
488515
487031
−2
1485


P3.orf0445
bam
Indoleacetamide hydrolase
488833
490233
1
1401


P3.orf0446
ytcJ
amidohydrolase-like
490463
492109
2
1647


P3.orf0447

cobalamin synthesis protein, P47K
492109
493038
1
930


P3.orf0448
ALD4
aldehyde dehydrogenase 5
493082
494605
2
1524


P3.orf0449

conserved hypothetical protein
494617
495354
1
738


P3.orf0450
appA
extracellular solute-binding protein family 5
495476
497071
2
1596


P3.orf0451
dppB
binding-protein dependent transport system
497178
498197
3
1020




inner membrane protein


P3.orf0452
appC
binding-protein-dependent transport systems
498194
499108
2
915




inner membrane component


P3.orf0453

oligopeptide/dipeptide ABC transporter,
499108
500118
1
1011




ATPase subunit


P3.orf0454
appF
oligopeptide/dipeptide ABC transporter,
500115
501104
3
990




ATPase subunit


P3.orf0455
acdS
1-aminocyclopropane-1-carboxylate
502175
501159
−3
1017




deaminase


P3.orf0456
lrp
leucine-responsive regulatory protein
502373
502843
2
471


P3.orf0457

NADH dehydrogenase protein
504282
502864
−1
1419


P3.orf0458

putative addiction module antidote protein,
505113
505406
3
294




CopG/Arc/MetJ family


P3.orf0459

conserved hypothetical protein
505091
504606
−3
486


P3.orf0460
yoaH
chemotaxis sensory transducer
506986
505490
−2
1497


P3.orf0461
glnQ
polar amino acid transport system ATP-
507949
507197
−2
753




binding protein


P3.orf0462

polar amino acid ABC transporter, inner
508586
507936
−3
651




membrane subunit


P3.orf0463
yecS
glutamine ABC superfamily ATP binding
509304
508579
−1
726




cassette transporter, membrane protein


P3.orf0464
glnH
amino acid ABC transporter substrate-binding
510214
509390
−2
825




protein


P3.orf0465
lutR
GntR domain-containing protein
510487
511170
1
684


P3.orf0466
alc1
allantoicase
511212
512225
3
1014


P3.orf0467
allA
ureidoglycolate hydrolase
512234
512761
2
528


P3.orf0468

hypothetical protein
514441
512849
−2
1593


P3.orf0469

hypothetical protein
514877
515026
2
150


P3.orf0470

hypothetical protein
514651
514505
−2
147


P3.orf0471
nrdZ
putative B12-dependent ribonucleoside-
516753
515536
−1
1218




diphosphate/-triphosphate reductase (nrdJ-




like)


P3.orf0472

hypothetical protein
517886
518038
2
153


P3.orf0473
ydcR
Bifunctional -- Transcriptional Regulator, GntR
519443
518043
−3
1401




family/Aminotransferase, class I and II


P3.orf0474

MFS transporter
519730
521019
1
1290


P3.orf0475

transcriptional regulator, BadM/Rrf2 family
521021
521401
2
381


P3.orf0476

HPr kinase
521401
522249
1
849


P3.orf0477

sulfotransferase
522246
523112
3
867


P3.orf0478
glcF
Glycolate oxidase iron-sulfur subunit
525337
523952
−2
1386


P3.orf0479
glcE
glycolate oxidase, subunit glcE
526566
525334
−1
1233


P3.orf0480
glcD
FAD linked oxidase-like protein
528058
526571
−2
1488


P3.orf0481
rpmJ
50S ribosomal protein L36
528391
528266
−2
126


P3.orf0482

hypothetical protein
528619
529317
1
699


P3.orf0483

Putative phosphoethanolamine N-
530285
529314
−3
972




methyltransferase


P3.orf0484
lcfA
acyl-CoA synthetase
532444
530696
−2
1749


P3.orf0485

biotin dependent acyl-CoA carboxylase
532599
534194
3
1596


P3.orf0486
des
fatty acid desaturase
534570
535637
3
1068


P3.orf0487

conserved hypothetical protein
536061
535885
−1
177


P3.orf0488

XRE family transcriptional regulator
537253
536912
−2
342


P3.orf0489

hypothetical protein
537835
537951
1
117


P3.orf0490

fad dependent oxidoreductase; protein
539165
537972
−3
1194


P3.orf0491

valyl-tRNA synthetase
541083
539137
−1
1947


P3.orf0492
betB
Aldehyde Dehydrogenase
542856
541297
−1
1560


P3.orf0493

6-phosphogluconate dehydrogenase NAD-
543742
542873
−2
870




binding


P3.orf0494
cynR
LysR family transcriptional regulator
543784
544932
1
1149


P3.orf0495

acetyltransferase (GNAT) family protein
545532
545140
−1
393


P3.orf0496

conserved hypothetical protein
545943
545662
−1
282


P3.orf0497

ABC transporter related protein
546858
546079
−1
780


P3.orf0498

binding-protein dependent transport system
547711
546917
−2
795




inner membrane protein


P3.orf0499
ssuA
putative sulfonate/nitrate transport system
548718
547735
−1
984




substrate-binding protein


P3.orf0500

agmatinase
549766
548759
−2
1008


P3.orf0501
cmpR
putative LysR-family regulatory protein
549871
550782
1
912


P3.orf0502

putative membrane protein
550993
551823
1
831


P3.orf0503

conserved hypothetical protein
551829
552332
3
504


P3.orf0504
sdaA
L-serine dehydratase 1
552433
553821
1
1389


P3.orf0505
ytnL
putative hydrolase
553879
555078
1
1200


P3.orf0506
dgdR
transcriptional regulator, LysR family
555974
555093
−3
882


P3.orf0507
icd
isocitrate dehydrogenase
556111
557196
1
1086


P3.orf0508

conserved hypothetical protein
557266
557913
1
648


P3.orf0509

methyl-accepting chemotaxis protein
559595
557919
−3
1677


P3.orf0510

methyl-accepting chemotaxis protein
561525
559840
−1
1686


P3.orf0511
mcp3
methyl-accepting chemotaxis sensory
563423
561738
−3
1686




transducer


P3.orf0512

anti-sigma factor, ChrR
563646
564329
3
684


P3.orf0513
yhbS
GCN5-related N-acetyltransferase
564359
564871
2
513


P3.orf0514
Aass
Alpha-aminoadipic semialdehyde synthase
565472
566539
2
1068


P3.orf0515

putative transcriptional regulator, AsnC family
565324
564887
−2
438




protein


P3.orf0516

CRISPR-associated protein Cas2
567419
567090
−3
330


P3.orf0517

conserved hypothetical protein
568244
567429
−3
816


P3.orf0518

conserved hypothetical protein
571430
568281
−3
3150


P3.orf0519

hypothetical protein
571876
571736
−2
141


P3.orf0520

putative ABC transporter-binding protein
572058
573365
3
1308


P3.orf0521

binding-protein-dependent transport systems
573362
574309
2
948




inner membrane component


P3.orf0522
yurM
maltose/maltodextrin ABC transporter
574306
575139
1
834




permease protein MalG


P3.orf0523
smoK
maltose
575149
576219
1
1071


P3.orf0524
rpfC
GAF sensor hybrid histidine kinase
576303
578552
3
2250


P3.orf0525
gstA
conserved hypothetical protein
578676
579287
3
612


P3.orf0526
dctA
sodium: dicarboxylate symporter
580834
579419
−2
1416


P3.orf0527
dctB
histidine kinase
581119
583020
1
1902


P3.orf0528
dctD
C4-dicarboxylate transport transcriptional
583017
584396
3
1380




regulatory protein dctD


P3.orf0529
MGLL
hydrolase
585291
584416
−1
876


P3.orf0530
ydaM
diguanylate cyclase
587186
585414
−3
1773


P3.orf0531
tnaA
tryptophanase
587561
589075
2
1515


P3.orf0532

conserved hypothetical protein
589207
589812
1
606


P3.orf0533
sodB
superoxide dismutase
589927
590535
1
609


P3.orf0534
trpI
LysR family transcriptional regulator
591413
590499
−3
915


P3.orf0535
pdxA
4-hydroxythreonine-4-phosphate
591591
592613
3
1023




dehydrogenase


P3.orf0536
ydbB
Cupin domain protein
592618
593676
1
1059


P3.orf0537

putative oxidoreductase protein
594448
593699
−2
750


P3.orf0538
yhjC
transcriptional regulator protein
594536
595471
2
936


P3.orf0539

transporter
596621
595425
−3
1197


P3.orf0540
pat
Phosphinothricin acetyltransferase
597202
596618
−2
585


P3.orf0541
cmpR
LysR family transcriptional regulator
597267
598265
3
999


P3.orf0542
ywnB
Rrf2-linked NADH-flavin reductase
599486
598875
−3
612


P3.orf0543
ytfH
HTH-type transcriptional regulator ytfH
599657
600055
2
399


P3.orf0544

TetR family transcriptional regulator
600669
600043
−1
627


P3.orf0545

putative NAD(P)H dehydrogenase
600774
601382
3
609


P3.orf0546

cyclase family protein
601466
602404
2
939


P3.orf0547
tpm
thiopurine S-methyltransferase
603073
602429
−2
645


P3.orf0548
moaR
transcriptional regulator, LuxR family
603210
603926
3
717


P3.orf0549

amine oxidase
605247
603910
−1
1338


P3.orf0550

short-chain dehydrogenase/reductase family
606076
605336
−2
741




oxidoreductase


P3.orf0551

AraC family transcriptional regulator
606190
607110
1
921


P3.orf0552

major facilitator superfamily MFS_1
607197
608450
3
1254


P3.orf0553
tdhA
TonB-dependent heme/hemoglobin receptor
610963
608438
−2
2526




family protein


P3.orf0554

FecR protein
611984
611058
−3
927


P3.orf0555

FecI-like protein
612481
611981
−2
501


P3.orf0556
ycdO
conserved hypothetical protein
613577
612753
−3
825


P3.orf0557
ycdB
Dyp-type peroxidase family protein
614927
613623
−3
1305


P3.orf0558
ycdO
conserved hypothetical protein
616107
614929
−1
1179


P3.orf0559
efeU
iron permease FTR1
616954
616109
−2
846


P3.orf0560

conserved hypothetical protein
623392
623982
1
591


P3.orf0561
pleC
sensor histidine kinase (non-motile and phage-
624021
625847
3
1827




resistance protein)


P3.orf0562
pleC
Signal transduction histidine kinase
627293
625860
−3
1434


P3.orf0563
purU
formyltetrahydrofolate deformylase
627659
628516
2
858


P3.orf0564

TRAP transporter solute receptor TAXI family
628659
629678
3
1020




protein


P3.orf0565

TRAP transporter, 4TM/12TM fusion protein
629779
631911
1
2133


P3.orf0566

conserved hypothetical protein
631908
632378
3
471


P3.orf0567

membrane protein-like protein
632378
633676
2
1299


P3.orf0568

cupin 2 domain-containing protein
633719
634216
2
498


P3.orf0569
fabG
short-chain dehydrogenase/reductase SDR
634978
634232
−2
747


P3.orf0570

hypothetical protein
635089
635322
1
234


P3.orf0571
luxQ
two-component hybrid sensor and regulator
635442
638924
3
3483


P3.orf0572
agmR
two-component response regulator
639596
638943
−3
654


P3.orf0573
proP
major facilitator superfamily
641507
639789
−3
1719


P3.orf0574

putative hydrolase/carboxylic esterase
642770
641817
−3
954


P3.orf0575

Leu/Ile/Val-binding protein
644023
642791
−2
1233


P3.orf0576
mexR
MarR family transcriptional regulator
644239
644751
1
513


P3.orf0577

conserved hypothetical protein
644833
645165
1
333


P3.orf0578

conserved hypothetical protein
645504
645773
3
270


P3.orf0579

transcriptional regulator, XRE family
645764
646111
2
348


P3.orf0580
yvoA
transcriptional regulator, GntR family
647380
648111
1
732


P3.orf0581

amidohydrolase 2
647292
646474
−1
819


P3.orf0582

conserved hypothetical protein
648226
649182
1
957


P3.orf0583

conserved hypothetical protein
649184
649621
2
438


P3.orf0584

integral membrane protein
649637
651130
2
1494


P3.orf0585

conserved hypothetical protein
651713
651135
−3
579


P3.orf0586
mnmA
tRNA (5-methylaminomethyl-2-thiouridylate)-
653006
651852
−3
1155




methyltransferase


P3.orf0587

Transcriptional activator, TenA family protein
653783
653061
−3
723


P3.orf0588
fdxB
2Fe—2S ferredoxin
654279
653947
−1
333


P3.orf0589

Iron-sulfur assembly protein
654862
654530
−2
333


P3.orf0590
mdtA
secretion protein
655170
656342
3
1173


P3.orf0591

hydrophobic/amphiphilic exporter-1
656364
659462
3
3099


P3.orf0592
ybaL
potassium efflux system protein
661960
660227
−2
1734


P3.orf0593

hypothetical protein
662198
662629
2
432


P3.orf0594
secD
protein-export membrane protein SECD
662767
665103
1
2337


P3.orf0595
grsT
Type II thioesterase
665186
665971
2
786


P3.orf0596
phbA
Membrane protease subunit
667426
665975
−2
1452




stomatin/prohibitin-like protein


P3.orf0597
yojI
Cyclic peptide transporter
669105
667423
−1
1683


P3.orf0598

hypothetical protein
669427
669215
−2
213


P3.orf0599

GTPase domain-containing protein
670123
669551
−2
573


P3.orf0600

cation/multidrug efflux pump protein
673337
670374
−3
2964


P3.orf0601

putative HlyD-like secretion protein
674600
673467
−3
1134


P3.orf0602
lgrC
OciB protein
674941
681312
1
6372


P3.orf0603
pksJ
linear gramicidin synthetase subunit D
681309
686699
3
5391


P3.orf0604
lgrC
non-ribosomal peptide synthetase
686696
690688
2
3993


P3.orf0605
lgrC
putative nonribosomal peptide synthetases
690664
702225
1
11562




(NPRS)


P3.orf0606
lgrC
erythronolide synthase
702250
707367
1
5118


P3.orf0607
pksJ
HctF
707383
712224
1
4842


P3.orf0608
pksJ
non-ribosomal peptide synthetase/polyketide
712221
716462
3
4242




synthase


P3.orf0609
lgrC
non-ribosomal peptide synthetase
716459
720319
2
3861


P3.orf0610
lgrC
non-ribosomal peptide synthetase
720291
722912
3
2622


P3.orf0611
lgrC
peptide synthetase
722909
729400
2
6492


P3.orf0612
mbtH
MbtH domain-containing protein
729464
729697
2
234


P3.orf0613

conserved hypothetical protein
731518
731312
−2
207


P3.orf0614

hypothetical protein
732225
732362
3
138


P3.orf0615

hypothetical protein
732366
732548
3
183


P3.orf0616

conserved hypothetical protein
733333
732761
−2
573


P3.orf0617

conserved hypothetical protein
734754
734527
−1
228


P3.orf0618
yyaK
CAAX amino terminal protease family
736217
735243
−3
975


P3.orf0619
yycB
cyanate transport system protein
737535
736339
−1
1197


P3.orf0620

transcriptional regulator, GntR family
738299
737532
−3
768


P3.orf0621

putative SURF1 family protein
739095
738403
−1
693


P3.orf0622
cyoD
cytochrome o ubiquinol oxidase subunit IV
739619
739194
−3
426


P3.orf0623
cyoC
cytochrome o ubiquinol oxidase subunit III
740278
739616
−2
663


P3.orf0624
cyoB
cytochrome o ubiquinol oxidase, subunit I
742286
740283
−3
2004




(ubiquinol oxidase chain A)


P3.orf0625
cyoA
ubiquinol oxidase, subunit II
743480
742305
−3
1176


P3.orf0626

type III effector protein
744090
744644
3
555


P3.orf0627

hypothetical protein
744655
745092
1
438


P3.orf0628

DNA methylase N-4/N-6
745248
748043
3
2796


P3.orf0629

type III restriction enzyme, res subunit
748063
751170
1
3108


P3.orf0630

UvrD/REP type DNA helicase
753318
751198
−1
2121


P3.orf0631
pdxJ
pyridoxal phosphate biosynthetic protein PdxJ
754149
753397
−1
753


P3.orf0632

hypothetical protein
754142
754318
2
177


P3.orf0633
ggpS
alpha,alpha-trehalose-phosphate synthase
755880
754360
−1
1521


P3.orf0634
ggpS
alpha,alpha-trehalose-phosphate synthase
756681
755959
−1
723


P3.orf0635
gpsA
glycerol-3-phosphate dehydrogenase
757862
756681
−3
1182


P3.orf0636
aglA
putative alpha-glucosidase AglA
758072
759703
2
1632


P3.orf0637

glutathione-dependent formaldehyde-
759738
760196
3
459




activating GFA


P3.orf0638

TetR family transcriptional regulator
760355
760999
2
645


P3.orf0639
ilvE
aminotransferase, class IV
761003
761953
2
951


P3.orf0640

conserved hypothetical protein
761953
763626
1
1674


P3.orf0641
crt
3-hydroxybutyryl-CoA dehydratase
764644
763850
−2
795


P3.orf0642
oruR
AraC family transcriptional regulator
764810
765886
2
1077


P3.orf0643

FAD/FMN-containing dehydrogenases
767279
765852
−3
1428


P3.orf0644
lutR
transcriptional regulator, GntR
767533
769188
1
1656




family/amidohydrolase family protein


P3.orf0645
yiaO
putative periplasmic substrate-binding
769391
770386
2
996




transport protein


P3.orf0646
siaT
TRAP-type C4-dicarboxylate transport system,
770492
770998
2
507




small permease component


P3.orf0647
ygiK
C4-dicarboxylate transport system (permease
770995
772278
1
1284




large protein)


P3.orf0648
cysB
cystathionine beta-synthase
772491
773513
3
1023


P3.orf0649

2-isopropylmalate synthase
773510
774922
2
1413


P3.orf0650

conserved hypothetical protein
774877
775938
1
1062


P3.orf0651

conserved hypothetical protein
776020
777183
1
1164


P3.orf0652

Argininosuccinate lyase 2
777193
778479
1
1287


P3.orf0653

conserved hypothetical protein
778476
779720
3
1245


P3.orf0654

hypothetical protein
779737
781011
1
1275


P3.orf0655

short chain dehydrogenase
782717
781806
−3
912


P3.orf0656

transcriptional regulator, TetR family
782822
783403
2
582


P3.orf0657
iclR
IclR family transcriptional regulator
784283
783417
−3
867


P3.orf0658

gentisate 1,2-dioxygenase
784491
785555
3
1065


P3.orf0659

fumarylacetoacetat hydroxylase
785552
786268
2
717


P3.orf0660
siaP
TRAP dicarboxylate transporter- DctP subunit
786340
787383
1
1044


P3.orf0661

hypothetical protein
787380
787946
3
567


P3.orf0662
siaT
trap dicarboxylate transporter, dctm subunit
787953
789257
3
1305


P3.orf0663
maiA
maleylacetoacetate isomerase
789271
789945
1
675


P3.orf0664

HTH-type transcriptional regulator
790355
790053
−3
303


P3.orf0665
iscS
Cysteine desulfurase
791652
790423
−1
1230


P3.orf0666
iscS
Cysteine sulfinate desulfinase/cysteine
792881
791745
−3
1137




desulfurase and related enzyme


P3.orf0667
iscR
transcriptional regulator, BadM/Rrf2 family
793399
792878
−2
522


P3.orf0668
cysE
serine O-acetyltransferase
794181
793441
−1
741


P3.orf0669

hydrolase of the alpha/beta superfamily
794379
795017
3
639


P3.orf0670
thrC
threonine synthase-like protein
796344
795088
−1
1257


P3.orf0671
siaT
TRAP transporter, 4TM/12TM fusion protein
798272
796416
−3
1857


P3.orf0672

TRAP transporter solute receptor, TAXI family
799414
798431
−2
984


P3.orf0673
kdgR
IclR family transcriptional regulator
800322
799525
−1
798


P3.orf0674
pepQ
peptidase M24
801581
800361
−3
1221


P3.orf0675
zntA
heavy metal translocating P-type ATPase
804002
801801
−3
2202


P3.orf0676
zntR
transcriptional regulator, MerR family protein
804141
804641
3
501


P3.orf0677
rsmA
phospholipid N-methyltransferase protein
804744
805394
3
651


P3.orf0678
anmK
anhydro-N-acetylmuramic acid kinase
807521
805377
−3
2145


P3.orf0679
tyrS
tyrosyl-tRNA synthetase
807709
808965
1
1257


P3.orf0680

YceI family protein
809682
809047
−1
636


P3.orf0681
slyA
MarR family transcriptional regulator
809891
810358
2
468


P3.orf0682

major facilitator transporter
810360
811934
3
1575


P3.orf0683
emrA
secretion protein HlyD
811942
812994
1
1053


P3.orf0684

conserved hypothetical protein
816400
813002
−2
3399


P3.orf0685
glnE
glutamate-ammonia-ligase adenylyltransferase
816637
819669
1
3033


P3.orf0686
bcp
Redoxin
819666
820127
3
462


P3.orf0687

conserved hypothetical protein
820143
821033
3
891


P3.orf0688
yncA
GCN5-related N-acetyltransferase
821069
821593
2
525


P3.orf0689

conserved hypothetical protein
821947
822381
1
435


P3.orf0690

conserved hypothetical protein
824286
823750
−1
537


P3.orf0691

conserved hypothetical protein
823753
822395
−2
1359


P3.orf0692

conserved hypothetical protein
824445
825173
3
729


P3.orf0693

major facilitator superfamily MFS_1
826398
825151
−1
1248


P3.orf0694
ydeM
putative acyl dehydratase
826905
826429
−1
477


P3.orf0695

ubiquinone/menaquinone biosynthesis
827735
826902
−3
834




methyltransferase protein


P3.orf0696

radical SAM domain protein
830039
827772
−3
2268


P3.orf0697
rhiR
LuxR family transcriptional regulator
830445
831188
3
744


P3.orf0698
rhlI
N-acyl-L-homoserine lactone synthetase MsaI
831221
831901
2
681


P3.orf0699
ydeM
MaoC domain protein dehydratase
831898
832386
1
489


P3.orf0700

alpha/beta hydrolase fold protein
833473
832415
−2
1059


P3.orf0701
prfB
peptide chain release factor RF-2
834438
833473
−1
966


P3.orf0702
mrcA
penicillin binding protein 1A
837211
834671
−2
2541


P3.orf0703
amiC
N-acetylmuramoyl-L-alanine amidase
838844
837453
−3
1392


P3.orf0704
rne
Ribonuclease E and G
839847
843209
3
3363


P3.orf0705
aspC
putative aminotransferase protein
844504
843335
−2
1170


P3.orf0706
GGT1
gamma-glutamyltranspeptidase precursor
845878
844637
−2
1242


P3.orf0707

putative Zn-dependent protease
845982
847358
3
1377


P3.orf0708

outer membrane protein
847424
848182
2
759


P3.orf0709
gph
phosphoglycolate phosphatase
848873
848187
−3
687


P3.orf0710

conserved hypothetical protein
849082
849558
1
477


P3.orf0711
vagC
SpoVT/AbrB-like protein
849653
849895
2
243


P3.orf0712

PilT protein domain protein
849994
850296
1
303


P3.orf0713

conserved hypothetical protein
850860
850378
−1
483


P3.orf0714
nahR
LysR family transcriptional regulator
850859
851845
2
987


P3.orf0715
bdlA
methyl-accepting chemotaxis sensory
852122
853828
2
1707




transducer with Pas/Pac sensor


P3.orf0716

fumarylacetoacetate hydrolase family protein
854906
853923
−3
984


P3.orf0717

homogentisate 12-dioxygenase
856060
854909
−2
1152


P3.orf0718
vllY
4-hydroxyphenylpyruvate dioxygenase
857153
856053
−3
1101


P3.orf0719

transcriptional regulator, MarR family protein
857344
857895
1
552


P3.orf0720

methyl-accepting chemotaxis sensory
858317
859390
2
1074




transducer


P3.orf0721
cya
adenylate cyclase protein
859494
861215
3
1722


P3.orf0722

Argininosuccinate lyase 2
862511
861219
−3
1293


P3.orf0723

conserved hypothetical protein
863485
862508
−2
978


P3.orf0724

fatty acid desaturase
864225
863482
−1
744


P3.orf0725

conserved hypothetical protein
865333
864473
−2
861


P3.orf0726
iolS
aldo/keto reductase
866343
865357
−1
987


P3.orf0727
lgrD
amino acid adenylation domain protein
869822
866340
−3
3483


P3.orf0728
gshB
glutathione synthase
871153
870191
−2
963


P3.orf0729
GST
glutathione S-transferase
872056
871313
−2
744


P3.orf0730
thiG
bifunctional sulfur carrier protein/thiazole
873100
872105
−2
996




synthase protein


P3.orf0731
aroQ
3-dehydroquinate dehydratase
873253
873732
1
480


P3.orf0732
accB
acetyl-CoA carboxylase, biotin carboxyl carrier
873725
874207
2
483




protein


P3.orf0733
accC
acetyl-CoA carboxylase
874221
875564
3
1344


P3.orf0734

D-serine dehydratase
875663
876799
2
1137


P3.orf0735
ycjX
conserved hypothetical protein
876931
878382
1
1452


P3.orf0736

membrane protein
878379
879446
3
1068


P3.orf0737

leucyl/phenylalanyl-tRNA--protein transferase
879579
880301
3
723


P3.orf0738

conserved hypothetical protein
880751
880308
−3
444


P3.orf0739

ABC superfamily ATP binding cassette
881269
880748
−2
522




transporter substrate binding protein


P3.orf0740

NADH dehydrogenase
881697
881350
−1
348


P3.orf0741
nrdJ
Ribonucleotide reductase large subunit
882342
886010
3
3669


P3.orf0742

putative aminopeptidase protein
886135
886365
1
231


P3.orf0743

domain of unknown function DUF1814
886362
887063
3
702


P3.orf0744

nuclease
888130
887069
−2
1062


P3.orf0745

conserved hypothetical protein
889257
888127
−1
1131


P3.orf0746

conserved hypothetical protein
890082
889258
−1
825


P3.orf0747

putative transmembrane protein
891508
890066
−2
1443


P3.orf0748

conserved hypothetical protein
892071
891652
−1
420


P3.orf0749
IivG
ABC transporter related protein
892719
893525
3
807


P3.orf0750
IivF
ABC transporter related protein
893545
894264
1
720


P3.orf0751
IivH
hydrophobic amino acid ABC transporter
894261
895124
3
864




permease


P3.orf0752
braE
inner-membrane translocator
895124
896110
2
987


P3.orf0753
nepI
major facilitator family transporter
897320
896151
−3
1170


P3.orf0754
yafC
Transcriptional regulator, LysR family
898325
897456
−3
870


P3.orf0755
mauR
LysR family transcriptional regulator
899245
898304
−2
942


P3.orf0756

sodium/sulphate symporter
899382
900788
3
1407


P3.orf0757

Glyoxalase/bleomycin resistance
901172
900798
−3
375




protein/dioxygenase


P3.orf0758

conserved hypothetical protein
902518
901277
−2
1242


P3.orf0759

sigma-54 interacting transcription regulator
902714
904321
2
1608




protein


P3.orf0760
nsrR
HTH-type transcriptional regulator nsrR
904774
904325
−2
450


P3.orf0761
braC
extracellular ligand-binding receptor
906172
904952
−2
1221


P3.orf0762

hypothetical protein
906686
906889
2
204


P3.orf0763
xdhA
aldehyde oxidase and xanthine
907429
910212
1
2784




dehydrogenase molybdopterin binding


P3.orf0764
hcrC
putative deshydrogenase/oxidoreductase;
910209
910781
3
573


P3.orf0765

Gluconate 2-dehydrogenase (acceptor)
910778
912058
2
1281


P3.orf0766

AMP-dependent synthetase and ligase
913887
912082
−1
1806


P3.orf0767
IivF
ABC transporter related protein
914711
913884
−3
828


P3.orf0768
IivK
Extracellular ligand-binding receptor
916006
914780
−2
1227


P3.orf0769
IivM
inner-membrane translocator
917130
916087
−1
1044


P3.orf0770
IivH
inner-membrane translocator
918063
917185
−1
879


P3.orf0771
braF
ABC transporter related protein
918916
918149
−2
768


P3.orf0772
yqjP
Beta-lactamase-like
920316
919276
−1
1041


P3.orf0773

acyl-CoA dehydrogenase
922322
920526
−3
1797


P3.orf0774

hypothetical protein
923029
922643
−2
387


P3.orf0775
asnO
asparagine synthase
925222
923261
−2
1962


P3.orf0776

conserved hypothetical protein
925873
925373
−2
501


P3.orf0777
sir1
sulfite reductase (ferredoxin)
927872
925857
−3
2016


P3.orf0778
cya
adenylate cyclase 1
929331
928132
−1
1200


P3.orf0779

conserved hypothetical protein
929415
929771
3
357


P3.orf0780

2OG-Fe(II) oxygenase
930407
929787
−3
621


P3.orf0781

conserved hypothetical protein
930792
932603
3
1812


P3.orf0782
mmgC
acyl-CoA dehydrogenase
934470
932680
−1
1791


P3.orf0783
fadN
3-hydroxyacyl-CoA dehydrogenase
936829
934496
−2
2334


P3.orf0784
fadA
3-ketoacyl-CoA thiolase
938014
936875
−2
1140


P3.orf0785

transcriptional regulator, MerR family protein
938540
938148
−3
393


P3.orf0786
lcfA
long-chain-fatty-acid--CoA ligase
940431
938701
−1
1731


P3.orf0787

Helix-turn-helix motif protein
941092
940724
−2
369


P3.orf0788

plasmid maintenance system killer
941296
941174
−2
123


P3.orf0789

hypothetical protein
942032
941577
−3
456


P3.orf0790

conserved hypothetical protein
942675
942043
−1
633


P3.orf0791

conserved hypothetical protein
943429
942773
−2
657


P3.orf0792

ArgK protein
944530
943568
−2
963


P3.orf0793
ppdK
pyruvate phosphate dikinase
947228
944574
−3
2655


P3.orf0794
glyS
glycyl-tRNA synthetase beta chain
949324
947249
−2
2076


P3.orf0795

hypothetical protein
950163
950405
3
243


P3.orf0796
glyQ
Glycyl-tRNA synthetase alpha chain
950135
949329
−3
807


P3.orf0797

hypothetical protein
950700
950449
−1
252


P3.orf0798

peptidase S49
951588
950713
−1
876


P3.orf0799

methyltransferase small
952466
951648
−3
819


P3.orf0800

conserved hypothetical protein
952771
952463
−2
309


P3.orf0801
ispB
Polyprenyl synthetase
953124
954125
3
1002


P3.orf0802
pleC
integral membrane sensor hybrid histidine
954513
955805
3
1293




kinase


P3.orf0803
ADS1
fatty-acid desaturase
955953
956948
3
996


P3.orf0804
ppsA
Beta-ketoacyl synthase
957140
957436
2
297


P3.orf0805

cyclopropane-fatty-acyl-phospholipid synthase
957464
958363
2
900


P3.orf0806

amino acid adenylation
958379
960163
2
1785


P3.orf0807

acyl-CoA dehydrogenase domain protein
960167
961330
2
1164


P3.orf0808
crt
F148266_1 unknown
961327
962502
1
1176


P3.orf0809
cmoA
methyltransferase type 12
963218
962508
−3
711


P3.orf0810

conserved hypothetical protein
963792
963313
−1
480


P3.orf0811

conserved hypothetical protein
964293
963898
−1
396


P3.orf0812

HDDC2 protein
964561
965175
1
615


P3.orf0813
ycfQ
transcriptional regulator, TetR family
965820
965188
−1
633


P3.orf0814
estB
beta-lactamase
965903
967150
2
1248


P3.orf0815

TetR-family transcriptional regulator
967743
967138
−1
606


P3.orf0816
gst3
glutathione S-transferase domain-containing
967843
968511
1
669




protein


P3.orf0817

conserved hypothetical protein
969588
968770
−1
819


P3.orf0818
yfhB
phenazine biosynthesis protein
971074
970151
−2
924


P3.orf0819

GntR family transcriptional regulator with
971121
972512
3
1392




aminotransferase domain


P3.orf0820

putative flagellin
972583
974163
1
1581


P3.orf0821

conserved hypothetical protein
974616
974173
−1
444


P3.orf0822
FMO5
Dimethylaniline monooxygenase [N-oxide-
976226
974622
−3
1605




forming] 5


P3.orf0823

biotin/lipoyl attachment domain-containing
976537
976319
−2
219




protein


P3.orf0824
accC
carbamoyl-phosphate synthase L chain ATP-
977933
976590
−3
1344




binding


P3.orf0825

conserved hypothetical protein
978049
978747
1
699


P3.orf0826

transcriptional regulator, XRE family
978834
979655
3
822


P3.orf0827
ybhB
putative phosphatidylethanolamine-binding
980209
979643
−2
567




protein


P3.orf0828
ybcM
AraC protein
981184
980306
−2
879


P3.orf0829

conserved hypothetical protein
981345
982268
3
924


P3.orf0830
rhtB
amino acid efflux protein
982913
982272
−3
642


P3.orf0831
mutB
methylmalonyl-CoA mutase
985204
983015
−2
2190


P3.orf0832
mutA
methylmalonyl-CoA mutase
987113
985209
−3
1905


P3.orf0833

Enoyl-CoA hydratase/isomerase
988058
987369
−3
690


P3.orf0834

hypothetical protein
988638
988207
−1
432


P3.orf0835

hypothetical protein
989373
988879
−1
495


P3.orf0836
hmuR
TonB-dependent receptor plug
989660
991966
2
2307


P3.orf0837
yddA
putative ABC transport system, ATP-binding
991971
993755
3
1785




protein


P3.orf0838
yrpG
putative oxidoreductase
994690
993740
−2
951


P3.orf0839

3-ketoacyl-(acyl-carrier-protein) reductase
995724
994966
−1
759


P3.orf0840

NHL repeat-containing protein
996113
997576
2
1464


P3.orf0841

GCN5-related N-acetyltransferase
997593
998120
3
528


P3.orf0842
npdA
NAD-dependent deacetylase
998882
998127
−3
756


P3.orf0843

nitro reductase
999578
998934
−3
645


P3.orf0844

hypothetical protein
999914
999597
−3
318


P3.orf0845
pleC
multi-sensor signal transduction histidine
1002236
1000074
−3
2163




kinase


P3.orf0846

ATP-dependent protease HslVU (ClpYQ),
1003513
1002365
−2
1149




ATPase subunit


P3.orf0847

cytochrome c family protein
1003815
1004216
3
402


P3.orf0848

conserved hypothetical protein
1004257
1004955
1
699


P3.orf0849
acoR
transcriptional regulator
1005170
1007215
2
2046


P3.orf0850
acoX
ATP-NAD/AcoX kinase
1007579
1008661
2
1083


P3.orf0851
acoA
acetoin dehydrogenase complex, E1
1008740
1009744
2
1005




component, alpha subunit


P3.orf0852
acoB
pyruvate dehydrogenase E1 component, beta
1009779
1010786
3
1008




subunit


P3.orf0853
acoC
branched-chain alpha-keto acid
1010805
1011917
3
1113




dehydrogenase subunit E2


P3.orf0854
budC
short-chain dehydrogenase/reductase SDR
1011922
1012716
1
795


P3.orf0855

hypothetical protein
1013102
1013245
2
144


P3.orf0856

Lysine-specific demethylase
1013381
1014550
2
1170


P3.orf0857

aspartyl/asparaginyl beta-hydroxylase
1015527
1014553
−1
975


P3.orf0858

conserved hypothetical protein
1015809
1015531
−1
279


P3.orf0859

prolyl 4-hydroxylase, alpha subunit
1015971
1016600
3
630


P3.orf0860

conserved hypothetical protein
1016930
1016616
−3
315


P3.orf0861
asnO
Asparagine synthase (glutamine-hydrolyzing)
1017430
1019361
1
1932


P3.orf0862

sulfotransferase
1020238
1019384
−2
855


P3.orf0863

HPr kinase
1021071
1020238
−1
834


P3.orf0864
viuB
siderophore-interacting protein
1022343
1021240
−1
1104


P3.orf0865

solute-binding periplasmic protein of
1023521
1022385
−3
1137




iron/siderophore ABC transporter


P3.orf0866
fhuA
OMR family ferrichrome outer membrane
1025656
1023536
−2
2121




transporter


P3.orf0867

TetR family transcriptional regulator
1025958
1026647
3
690


P3.orf0868

alpha/beta hydrolase fold
1027782
1026658
−1
1125


P3.orf0869
paaG
enoyl-CoA hydratase
1028657
1027845
−3
813


P3.orf0870

Acetyl-CoA C-acetyltransferase
1029949
1028765
−2
1185


P3.orf0871
yncB
oxidoreductase, zinc-binding dehydrogenase
1030187
1031251
2
1065




family


P3.orf0872
pleC
two-component sensor histidine kinase
1031517
1032734
3
1218


P3.orf0873

putative lipoprotein
1033471
1032830
−2
642


P3.orf0874

hypothetical protein
1033737
1033501
−1
237


P3.orf0875

putative 2-hydroxychromene-2-carboxylate
1034622
1034008
−1
615




isomerase


P3.orf0876

5-carboxymethyl-2-hydroxymuconate delta-
1034781
1035623
3
843




isomerase


P3.orf0877
yjcC
conserved hypothetical protein
1035829
1037511
1
1683


P3.orf0878

conserved hypothetical protein
1038050
1037535
−3
516


P3.orf0879

transcriptional regulator, XRE family
1038394
1038999
1
606


P3.orf0880

sulfonate/nitrate/taurine transport system ATP-
1039664
1040437
2
774




binding protein


P3.orf0881

sulfonate/nitrate/taurine transport system
1040430
1041365
3
936




permease protein


P3.orf0882

sulfonate/nitrate/taurine transport system
1041362
1042516
2
1155




permease protein


P3.orf0883

sulfonate/nitrate/taurine transport system
1042687
1043700
1
1014




substrate-binding protein


P3.orf0884

diguanylate cyclase/phosphodiesterase with
1043849
1045435
2
1587




PAS/PAC sensor(s)


P3.orf0885
ytsP
conserved hypothetical protein
1045456
1045956
1
501


P3.orf0886
yhjX
major facilitator superfamily MFS_1
1047664
1046012
−2
1653


P3.orf0887
cynR
LysR-family transcriptional regulator
1048839
1047913
−1
927


P3.orf0888
yhcA
EmrB/QacA family drug resistance transporter
1050376
1048943
−2
1434


P3.orf0889
slyA
regulatory protein, MarR
1050906
1050373
−1
534


P3.orf0890
kipR
IclR family regulatory protein
1051954
1051049
−2
906


P3.orf0891
acoA
Thiamine pyrophosphate-dependent
1052403
1053386
3
984




dehydrogenase, E1 component alpha subunit


P3.orf0892
pdhB
putative pyruvate dehydrogenase E1 beta
1053383
1054354
2
972




subunit


P3.orf0893

outer membrane efflux protein
1056161
1054611
−3
1551


P3.orf0894
stan
VCBS
1059657
1056286
−1
3372


P3.orf0895
Fat4
Putative Ig domain family
1076476
1059644
−2
16833


P3.orf0896

conserved hypothetical protein
1082145
1076527
−1
5619


P3.orf0897

conserved hypothetical protein
1082623
1082946
1
324


P3.orf0898

Tail Collar domain protein
1082954
1083649
2
696


P3.orf0899

tail collar domain-containing protein
1083750
1084343
3
594


P3.orf0900

phage tail collar domain-containing protein
1084375
1084974
1
600


P3.orf0901

phage tail collar domain-containing protein
1085296
1085622
1
327


P3.orf0902

peptidase M50
1088761
1086569
−2
2193


P3.orf0903
mdtA
Multidrug resistance protein mdtA
1090330
1088765
−2
1566


P3.orf0904

conserved hypothetical protein
1091271
1090327
−1
945


P3.orf0905

conserved hypothetical protein
1092302
1091397
−3
906


P3.orf0906
sam
S-adenosylmethionine uptake transporter
1092538
1093491
1
954


P3.orf0907
yodQ
acetylornithine deacetylase or succinyl-
1093542
1094795
3
1254




diaminopimelate desuccinylase


P3.orf0908

conserved hypothetical protein
1095826
1095146
−2
681


P3.orf0909
ydjG
aldo/keto reductase
1096797
1095826
−1
972


P3.orf0910

membrane protein
1097557
1096910
−2
648


P3.orf0911

hypothetical protein
1097741
1097571
−3
171


P3.orf0912

hypothetical protein
1098183
1097731
−1
453


P3.orf0913
fabG
short-chain dehydrogenase/reductase SDR
1099050
1098265
−1
786


P3.orf0914
linX
short-chain dehydrogenase/reductase SDR
1099871
1099077
−3
795


P3.orf0915
yiaN
DctM9
1101186
1099900
−1
1287


P3.orf0916

DctQ9
1101722
1101183
−3
540


P3.orf0917
siaP
TRAP dicarboxylate transporter, DctP subunit
1102788
1101802
−1
987


P3.orf0918

hypothetical protein
1103201
1103016
−3
186


P3.orf0919
fixI
cation transport ATPase
1105679
1103229
−3
2451


P3.orf0920

nitrogen fixation protein fixH
1106284
1105700
−2
585


P3.orf0921
fixG
nitrogen fixation protein fixG
1107868
1106360
−2
1509


P3.orf0922
petJ
cytochrome-c oxidase fixP
1108809
1107940
−1
870


P3.orf0923

cytochrome-c oxidase FixO2
1109728
1109003
−2
726


P3.orf0924
fixN
cbb3-type cytochrome c oxidase subunit I
1111223
1109733
−3
1491


P3.orf0925

hypothetical protein
1111513
1111256
−2
258


P3.orf0926

conserved hypothetical protein
1111787
1112620
2
834


P3.orf0927

hypothetical protein
1112860
1112642
−2
219


P3.orf0928
IivF
ABC transporter ATP-binding protein
1113652
1112957
−2
696


P3.orf0929
IivG
ABC transporter ATP-binding protein
1114401
1113649
−1
753


P3.orf0930

ABC transporter permease protein
1115462
1114398
−3
1065


P3.orf0931
IivH
inner-membrane translocator
1116426
1115491
−1
936


P3.orf0932

Extracellular ligand-binding receptor
1117676
1116489
−3
1188


P3.orf0933

transcriptional regulator, AsnC family protein
1118010
1117768
−1
243


P3.orf0934

conserved hypothetical protein
1119103
1119681
1
579


P3.orf0935

GCN5-related N-acetyltransferase
1119806
1121011
2
1206


P3.orf0936
lysX
RimK domain protein ATP-grasp
1121016
1122521
3
1506


P3.orf0937
yncD
putative TonB-dependent receptor protein
1122565
1124988
1
2424


P3.orf0938

transcriptional regulator
1125283
1124993
−2
291


P3.orf0939

addiction module killer protein
1125581
1125276
−3
306


P3.orf0940

thioesterase superfamily protein
1126135
1125626
−2
510


P3.orf0941
menE
O-succinylbenzoate--CoA ligase
1126640
1126176
−3
465


P3.orf0942
fadD
AMP-dependent synthetase and ligase
1126818
1126660
−1
159


P4.orf0001
hcpC
Putative beta-lactamase hcpC
1
1233
1
1233


P4.orf0002

rifampin ADP-ribosylating transferase
1724
1275
−3
450


P4.orf0003

conserved hypothetical protein
2205
1702
−1
504


P4.orf0004

ThiJ/PfpI
3391
2255
−2
1137


P4.orf0005
glxA
transcriptional regulator, AraC family
3495
4505
3
1011


P4.orf0006
pleC
Signal transduction histidine kinase
6703
4697
−2
2007


P4.orf0007

putative endonuclease involved in
7431
6823
−1
609




recombination


P4.orf0008
gatC
aspartyl/glutamyl-tRNA(Asn/Gln)
7647
7934
3
288




amidotransferase subunit C


P4.orf0009
gatA
aspartyl/glutamyl-tRNA(Asn/Gln)
7931
9433
2
1503




amidotransferase subunit A


P4.orf0010
gatB
aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln)
9430
10881
1
1452




amidotransferase subunit B


P4.orf0011

hypothetical protein
11112
11450
3
339


P4.orf0012

Putative lipoprotein
11505
12014
3
510


P4.orf0013
yafP
N-acetyltransferase yafP
12042
12503
3
462


P4.orf0014

CopG family DNA-binding protein
12528
12803
3
276


P4.orf0015

hypothetical protein
13120
12971
−2
150


P4.orf0016

toxin complex protein
13164
20768
3
7605


P4.orf0017
metI
binding-protein-dependent transport systems
21511
20828
−2
684




inner membrane component


P4.orf0018
metN
D-methionine transport system ATP-binding
22577
21495
−3
1083




protein


P4.orf0019
ygcF
7-carboxy-7-deazaguanine synthase homolog
22800
23543
3
744


P4.orf0020
queD
putative 6-pyruvoyl tetrahydropterin synthase
23543
23929
2
387


P4.orf0021
queC
exsB protein
23926
24669
1
744


P4.orf0022

23S rRNA methyltransferase
24686
25204
2
519


P4.orf0023
menE
AMP-dependent synthetase and ligase
26973
25480
−1
1494


P4.orf0024
fadR
transcriptional regulator AcrR family
27670
27053
−2
618


P4.orf0025

conserved hypothetical protein
28000
28827
1
828


P4.orf0026

putative Rossmann fold nucleotide-binding
29784
30428
3
645




protein


P4.orf0027
glpR
transcriptional regulator
29699
28854
−3
846


P4.orf0028
phnV
ABC transporter permease protein
30599
31381
2
783


P4.orf0029
potA
ABC transporter ATP-binding protein
31392
32417
3
1026


P4.orf0030

spermidine/putrescine transport system
32495
33592
2
1098




substrate-binding protein


P4.orf0031
potB
Spermidine/putrescine transport system
33705
34526
3
822




permease protein


P4.orf0032
siaP
TRAP-type bacterial extracellular solute-
35753
34626
−3
1128




binding protein


P4.orf0033
siaT
TrapT family protein
37113
35791
−1
1323


P4.orf0034

Tripartite ATP-independent periplasmic
37598
37110
−3
489


P4.orf0035
glpR
putative transcriptional regulator
38445
37690
−1
756


P4.orf0036

oxidoreductase, FAD-binding protein
38634
40082
3
1449


P4.orf0037
ooxA
Opine oxidase subunit A
40100
41329
2
1230


P4.orf0038
glpK
glycerol kinase
41326
42756
1
1431


P4.orf0039

conserved hypothetical protein
43607
42732
−3
876


P4.orf0040

hypothetical protein
43635
43880
3
246


P4.orf0041
citE
Citrate lyase
44886
43918
−1
969


P4.orf0042
puuR
XRE family-like protein
45473
44883
−3
591


P4.orf0043
artJ
ABC-type amino acid transport
45870
46616
3
747


P4.orf0044
glnM
amino acid ABC transporter permease protein
46777
47400
1
624


P4.orf0045
tcyC
amino acid ABC transporter ATP-binding
47397
48128
3
732




protein


P4.orf0046
puuB
FAD dependent oxidoreductase
48489
49658
3
1170


P4.orf0047

putative bleomycin resistance protein
50018
49659
−3
360


P4.orf0048
fhuC
ABC transporter related protein
50845
50063
−2
783


P4.orf0049
yojI
pyoverdine biosynthesis protein PvdE
52485
50845
−1
1641


P4.orf0050
viuB
FAD-binding 9 siderophore-interacting
53240
52482
−3
759




domain-containing protein


P4.orf0051
fhuB
transport system permease protein
55216
53237
−2
1980


P4.orf0052
fhuD
iron complex transport system substrate-
56103
55213
−1
891




binding protein


P4.orf0053
foxA
putative TonB-dependent receptor
58628
56106
−3
2523


P4.orf0054
fecR
FecR protein
59723
58737
−3
987


P4.orf0055
sigW
DNA-directed RNA polymerase specialized
60330
59815
−1
516




sigma subunit


P4.orf0056

conserved hypothetical protein
60860
60522
−3
339


P4.orf0057

conserved hypothetical protein
61234
62583
1
1350


P4.orf0058

mrp protein
63861
62650
−1
1212


P4.orf0059
hflK
HflK protein
64296
65366
3
1071


P4.orf0060
hflC
membrane protease subunit HflC
65385
66287
3
903


P4.orf0061

conserved hypothetical protein
66495
66776
3
282


P4.orf0062

conserved hypothetical protein
67990
68175
1
186


P4.orf0063
degP
Peptidase S1C, Do
68267
69739
2
1473


P4.orf0064
tas
aldo/keto reductase
67819
66809
−2
1011


P4.orf0065

phosphoserine phosphatase SerB
70742
69825
−3
918


P4.orf0066
miaA
tRNA delta(2)-isopentenylpyrophosphate
70814
71782
2
969




transferase


P4.orf0067
ilvI
acetolactate synthase, large subunit
72021
73772
3
1752


P4.orf0068
ilvH
acetolactate synthase 3 regulatory subunit
73882
74418
1
537


P4.orf0069
ilvC
ketol-acid reductoisomerase
74480
75607
2
1128


P4.orf0070
yfkH
ribonuclease BN
75627
76763
3
1137


P4.orf0071

conserved hypothetical protein
77386
76790
−2
597


P4.orf0072

conserved hypothetical protein
77913
77488
−1
426


TM.orf0001
dnaA
chromosomal replication initiation protein
1
1578
1
1578


TM.orf0002
dnaN
DNA polymerase III subunit beta
1786
2907
1
1122


TM.orf0003
recF
recombination protein F
2904
4169
2
1266


TM.orf0004
gyrB
DNA gyrase subunit B
4183
6687
1
2505


TM.orf0005

putative transcription regulator protein
6772
6981
1
210


TM.orf0006

conserved hypothetical protein
6978
7244
2
267


TM.orf0007
ygiD
Catalytic LigB subunit of aromatic ring-opening
8093
7278
−2
816




dioxygenase


TM.orf0008
pbpG
penicillin binding protein
8240
10417
3
2178


TM.orf0009
bcr
drug resistance transporter, Bcr/CflA subfamily
11649
10429
−1
1221


TM.orf0010
citA
Citrate (Si)-synthase
12169
13266
1
1098


TM.orf0011

conserved hypothetical protein
14238
13351
−1
888


TM.orf0012

2-amino-3-carboxymuconate-6-semialdehyde
15546
14329
−1
1218




decarboxylase


TM.orf0013
leuA
pyruvate carboxyltransferase
16189
17394
1
1206


TM.orf0014

Pc06g00060
17400
18092
2
693


TM.orf0015

CADD protein
18203
18883
3
681


TM.orf0016
potC
binding-protein-dependent transport systems
19730
18945
−2
786




inner membrane component


TM.orf0017
potH
binding-protein-dependent transport systems
20912
19737
−2
1176




inner membrane component


TM.orf0018
potA
putative spermidine/putrescine ABC
21979
20909
−3
1071




transporter, ATP-binding protein


TM.orf0019

spermidine/putrescine-binding periplasmic
23147
22092
−2
1056




protein


TM.orf0020

ABC-2 type transporter
24256
23381
−3
876


TM.orf0021
nodI
ABC transporter related protein
25005
24253
−1
753


TM.orf0022

conserved hypothetical protein
25155
25655
2
501


TM.orf0023
regB
two-component sensor histidine kinase
25652
27001
3
1350


TM.orf0024
regA
photosynthetic apparatus regulatory protein
27055
27645
1
591




RegA


TM.orf0025
yneP
thioesterase family protein
27784
28308
1
525


TM.orf0026
mprA
two component transcriptional regulator,
29239
28325
−3
915




winged helix family


TM.orf0027
argD
acetylornithine and succinylornithine
29533
30732
1
1200




aminotransferase


TM.orf0028
argF
ornithine carbamoyltransferase
30729
31256
2
528


TM.orf0029
argF
ornithine carbamoyltransferase
31267
31704
1
438


TM.orf0030
hslO
molecular chaperone Hsp33
31701
32687
2
987


TM.orf0031

Acyl-CoA synthetases (AMP-forming)/AMP-
32743
33399
1
657




acid ligases II


TM.orf0032
gylR
IclR family regulatory protein
33543
34448
2
906


TM.orf0033
menC
N-acylamino acid racemase
35583
34474
−1
1110


TM.orf0034

beta-lactamase
37011
35704
−1
1308


TM.orf0035
nudF
adp-ribose pyrophosphatase
37620
37156
−1
465


TM.orf0036

TetR family transcriptional regulator
38271
37663
−1
609


TM.orf0037
crp
transcriptional regulator
38805
39485
2
681


TM.orf0038
fixR
halohydrin epoxidase A
39570
40316
2
747


TM.orf0039

hypothetical protein
40712
40512
−2
201


TM.orf0040

secretion activator protein
41274
40729
−1
546


TM.orf0041

hypothetical protein
41399
41271
−2
129


TM.orf0042

putative tail fiber protein
43942
41588
−3
2355


TM.orf0043

conserved hypothetical protein
46922
43947
−2
2976


TM.orf0044

conserved hypothetical protein
49201
46919
−3
2283


TM.orf0045

conserved hypothetical protein
52092
49198
−1
2895


TM.orf0046

hypothetical protein
52646
52092
−2
555


TM.orf0047

conserved hypothetical protein
54580
52646
−3
1935


TM.orf0048

conserved hypothetical protein
55198
54584
−3
615


TM.orf0049

hypothetical protein
55520
55203
−2
318


TM.orf0050

conserved hypothetical protein
56404
55535
−3
870


TM.orf0051

hypothetical protein
57586
56708
−3
879


TM.orf0052

hypothetical protein
57893
57606
−2
288


TM.orf0053

conserved hypothetical protein
59500
57890
−3
1611


TM.orf0054

conserved hypothetical protein
60285
59713
−1
573


TM.orf0055

conserved hypothetical protein
61524
60295
−1
1230


TM.orf0056

conserved hypothetical protein
62297
61770
−2
528


TM.orf0057

DNA primase
64465
62915
−3
1551


TM.orf0058

conserved hypothetical protein
65855
64749
−2
1107


TM.orf0059

hypothetical protein
65984
66289
3
306


TM.orf0060

hypothetical protein
66645
66322
−1
324


TM.orf0061

conserved hypothetical protein
66920
66645
−2
276


TM.orf0062

hypothetical protein
67428
67204
−1
225


TM.orf0063

conserved hypothetical protein
67775
67425
−2
351


TM.orf0064

hypothetical protein
68454
68194
−1
261


TM.orf0065

conserved hypothetical protein
68687
68451
−2
237


TM.orf0066

hypothetical protein
69642
69863
2
222


TM.orf0067

hypothetical protein
69856
70269
1
414


TM.orf0068

conserved hypothetical protein
70675
71391
1
717


TM.orf0069

hypothetical protein
71388
71807
2
420


TM.orf0070

hypothetical protein
71804
72085
3
282


TM.orf0071

hypothetical protein
72105
72434
2
330


TM.orf0072

hypothetical protein
72421
72678
1
258


TM.orf0073

hypothetical protein
72678
72995
2
318


TM.orf0074

phage integrase family protein
72995
74203
3
1209


TM.orf0075

hypothetical protein
74694
74437
−1
258


TM.orf0076

hypothetical protein
75004
75219
1
216


TM.orf0077

hypothetical protein
75230
75640
3
411


TM.orf0078

conserved hypothetical protein
75646
75903
1
258


TM.orf0079

L-lactate permease
77675
76167
−2
1509


TM.orf0080

hypothetical protein
77831
79300
3
1470


TM.orf0081
fh
fumarate hydratase, class II
80821
79415
−3
1407


TM.orf0082

HemY protein
82392
80995
−1
1398


TM.orf0083

conserved hypothetical protein
83888
82389
−2
1500


TM.orf0084
hemD
uroporphyrinogen III synthase HEM4
84784
84008
−3
777


TM.orf0085
HEMC
Porphobilinogen deaminase, chloroplastic
85834
84845
−3
990


TM.orf0086
gcp
O-sialoglycoprotein endopeptidase
85940
87097
3
1158


TM.orf0097

conserved hypothetical protein
88155
87109
−1
1047


TM.orf0088

conserved hypothetical protein
89324
88152
−2
1173


TM.orf0089

conserved hypothetical protein
90512
89349
−2
1164


TM.orf0090

transmembrane protein
91469
90630
−2
840


TM.orf0091

NmrA-like protein
92583
91600
−1
984


TM.orf0092
ycil
YCIl-related protein
92841
93125
2
285


TM.orf0093

Thymocyte nuclear protein
93147
93566
2
420


TM.orf0094
petC
Rieske
93568
93927
1
360


TM.orf0095
acsA
acetyl-coenzyme A synthetase
94040
95998
3
1959


TM.orf0096

GMC oxidoreductase
97714
96806
−3
909


TM.orf0097

hypothetical protein
98517
98398
−1
120


TM.orf0098

conserved hypothetical protein
99271
98888
−3
384


TM.orf0099

conserved hypothetical protein
99605
99426
−2
180


TM.orf0100
htpX
heat shock protein HtpX
99762
100673
2
912


TM.orf0101
rsmB
Sun protein
100681
102306
1
1626


TM.orf0102
rpe
ribulose-phosphate 3-epimerase
102358
103083
1
726


TM.orf0103

conserved hypothetical protein
103162
105234
1
2073


TM.orf0104
rrf2
FeS assembly SUF system regulator
105385
105822
1
438


TM.orf0105

FeS assembly protein SufB
105949
107394
1
1446


TM.orf0106
sufC
FeS assembly ATPase SufC
107488
108246
1
759


TM.orf0107

FeS assembly protein SufD
108251
109600
3
1350


TM.orf0108
sufS
cysteine desulfurase, SufS subfamily
109597
110844
1
1248


TM.orf0109
nifU
NifU family SUF system FeS assembly protein
110856
111359
2
504


TM.orf0110
yitW
conserved hypothetical protein
111435
111776
2
342


TM.orf0111

conserved hypothetical protein
111898
112584
1
687


TM.orf0112
mhqR
transcriptional regulator, MarR family
112665
113168
2
504


TM.orf0113

conserved hypothetical protein
113153
113515
3
363


TM.orf0114
ydfH
transcriptional regulatory protein
114188
113553
−2
636


TM.orf0115

choline/carnitine/betaine transporter
116182
114206
−3
1977


TM.orf0116

hypothetical protein
116447
116590
3
144


TM.orf0117
ybbK
conserved hypothetical protein
117109
116603
−3
507


TM.orf0118
meaA
methylmalonyl-CoA mutase
119139
117109
−1
2031


TM.orf0119

crotonyl-CoA reductase
119561
120847
3
1287


TM.orf0120
yngJ
Acyl-CoA dehydrogenase
121098
122777
2
1680


TM.orf0121

hypothetical protein
122896
123093
1
198


TM.orf0122

ATP: cob(I)alamin adenosyltransferase
123188
123763
3
576


TM.orf0123
etfB
Electron transfer flavoprotein, beta subunit
124053
124802
2
750


TM.orf0124
etfA
electron transfer flavoprotein alpha subunit
124805
125737
3
933


TM.orf0125
hbdA
3-hydroxyacyl-CoA dehydrogenase
125872
126744
1
873


TM.orf0126

Thiol: disulfide interchange protein TlpA
127403
126807
−2
597


TM.orf0127
argH
argininosuccinate lyase
127533
128948
2
1416


TM.orf0128
lysA
diaminopimelate decarboxylase
129193
130452
1
1260


TM.orf0129

hypoxanthine phosphoribosyltransferase
131087
130545
−2
543


TM.orf0130

conserved hypothetical protein
131816
131157
−2
660


TM.orf0131
ftsE
putative ATPase involved in cell division
132046
132810
1
765


TM.orf0132

cell division protein
132807
133694
2
888


TM.orf0133

conserved hypothetical protein
133763
134383
3
621


TM.orf0134

phospholipid/glycerol acyltransferase
134380
135102
1
723


TM.orf0135
yigI
thioesterase superfamily protein
135712
135278
−3
435


TM.orf0136

thioesterase superfamily protein
136197
135709
−1
489


TM.orf0137
chaC
Cation transport protein chaC
136860
136246
−1
615


TM.orf0138

conserved hypothetical protein
137089
138096
1
1008


TM.orf0139
tyrC
cyclohexadienyl dehydrogenase
139029
138133
−1
897


TM.orf0140
hisC
histidinol-phosphate aminotransferase
140208
139108
−1
1101


TM.orf0141
pheA
chorismate mutase
141267
140347
−1
921


TM.orf0142
metX
homoserine O-acetyltransferase
141696
142784
2
1089


TM.orf0143

putative methionine biosynthesis protein
142781
143473
3
693




(MetW)


TM.orf0144
pleC
non-motile and phage-resistance protein
144628
143483
−3
1146


TM.orf0145
yiaO
TRAP dicarboxylate transporter, DctP subunit
145134
146123
2
990


TM.orf0146

tripartite ATP-independent periplasmic
146223
146735
2
513




transporter DctQ


TM.orf0147

TRAP dicarboxylate transporter, DctM subunit
146752
148017
1
1266


TM.orf0148

SAM-dependent methyltransferases
148765
147989
−3
777


TM.orf0149
gloB
metallo-beta-lactamase family protein
148965
149744
2
780


TM.orf0150
yibF
Glutathione S-transferase
149898
150503
2
606


TM.orf0151

hypothetical protein
150954
150544
−1
411


TM.orf0152

conserved hypothetical protein
151812
151060
−1
753


TM.orf0153
phbB
acetoacetyl-CoA reductase
152634
151909
−1
726


TM.orf0154
phaA
acetyl-CoA acetyltransferase
153993
152821
−1
1173


TM.orf0155
phbC
polyhydroxyalkanoate synthase
155401
154136
−3
1266


TM.orf0156

polyhydroxyalkanoate synthesis repressor
156093
156713
2
621




PhaR


TM.orf0157

conserved hypothetical protein
157735
156914
−3
822


TM.orf0158
arcB
PAS/PAC sensor hybrid histidine kinase
161874
157891
−1
3984


TM.orf0159

hypothetical protein
162127
162324
1
198


TM.orf0160

ABC superfamily ATP binding cassette
162639
163628
2
990




transporter, binding protein


TM.orf0161

ABC-type transport system, permease
164038
164925
1
888




component


TM.orf0162

Cobalt import ATP-binding protein cbiO 2
164944
165738
1
795


TM.orf0163

NUDIX hydrolase
165845
166438
3
594


TM.orf0164

NHL repeat-containing protein
166621
168297
1
1677


TM.orf0165
ispZ
intracellular septation protein
168936
168310
−1
627


TM.orf0166
ftsY
Signal recognition particle GTPase
169885
168941
−3
945


TM.orf0167

MiaB-like tRNA modifying enzyme
171300
169882
−1
1419


TM.orf0168
dapF
diaminopimelate epimerase
172243
171332
−3
912


TM.orf0169
divL
sensor protein divL
174357
172282
−1
2076


TM.orf0170
ahcY
S-adenosyl-L-homocysteine hydrolase
175792
174497
−3
1296


TM.orf0171
ptsH
phosphocarrier protein HPr
176294
175995
−2
300


TM.orf0172
manX
PTS system, IIA component
176722
176315
−3
408


TM.orf0173

3448_RHORT RecName: Full = UPF0042
177782
176838
−2
945




nucleotide-binding protein Rru_A3448


TM.orf0174
hprK
HPr kinase
178240
177779
−3
462


TM.orf0175
ptsN
phosphotransferase system mannitol/fructose-
178760
178296
−2
465




specific IIA domain-containing protein


TM.orf0176

sigma 54 modulation protein
179526
178927
−1
600


TM.orf0177

RNA polymerase factor sigma-54
181269
179695
−1
1575


TM.orf0178

ABC transporter, ATP-binding protein
182080
181280
−3
801


TM.orf0179

conserved hypothetical protein
182789
182148
−2
642


TM.orf0180

lipopolysaccharide export system protein LptC
183433
182804
−3
630


TM.orf0181

Predicted sugar phosphate isomerase
184530
183538
−1
993




involved in capsule formation


TM.orf0182
rnd
3′-5′ exonuclease
185288
184677
−2
612


TM.orf0183

NADH-ubiquinone oxidoreductase 39 kDa
185536
186507
1
972




subunit precursor


TM.orf0184
gltD
putative oxidoreductase
187193
188638
3
1446


TM.orf0185
gltB
glutamate synthase(NADPH) large subunit
188662
193212
1
4551


TM.orf0186
gdhA
Glu/Leu/Phe/Val dehydrogenase
193479
194759
2
1281


TM.orf0187

RarD protein
194918
195850
3
933


TM.orf0188

glutathione S-transferase
195862
196530
1
669


TM.orf0189
yjeS
putative iron-sulfur cluster binding protein
196511
197725
3
1215


TM.orf0190
yeeZ
NAD-dependent epimerase/dehydratase
197722
198687
1
966


TM.orf0191

ABC transporter, ATP-binding protein
200274
198694
−1
1581


TM.orf0192

hydrolase
201221
200403
−2
819


TM.orf0193

Glycosyltransferase
201550
202644
1
1095


TM.orf0194
rfaF
ADP-heptose of LPS heptosyltransferase
202641
203612
2
972


TM.orf0195
infC
translation initiation factor IF-3
203868
204446
2
579


TM.orf0196
rpmI
50S ribosomal protein L35
204715
204912
1
198


TM.orf0197
rplT
ribosomal protein L20
204999
205367
2
369


TM.orf0198
pheS
phenylalanyl-tRNA synthetase alpha chain
205622
206701
3
1080


TM.orf0199
pheT
phenylalanyl-tRNA synthetase beta chain
206718
209126
2
2409


TM.orf0200
cyaA
putative adenylate cyclase
209411
210817
3
1407


TM.orf0201
lepA
GTP-binding protein
210967
212766
1
1800


TM.orf0202

hypothetical protein
213156
212833
−1
324


TM.orf0203

major facilitator superfamily MFS_1
214904
213621
−2
1284


TM.orf0204

regulatory protein ArsR
215206
214874
−3
333


TM.orf0205
icfA
Putative carbonic anhydrase precursor
216181
215435
−3
747


TM.orf0206
yxbA
conserved hypothetical protein
216380
216661
3
282


TM.orf0207

GCN5-related N-acetyltransferase
216787
217368
1
582


TM.orf0208

conserved hypothetical protein
217661
217350
−2
312


TM.orf0209

microcystin-dependent protein
218298
218855
2
558


TM.orf0210

putative microcystin dependent protein
218946
219497
2
552


TM.orf0211

tail collar domain-containing protein
219511
220071
1
561


TM.orf0212

cytochrome B561
220281
220988
2
708


TM.orf0213

cytochrome C
221508
221068
−1
441


TM.orf0214
mcp4
Pmethyl-accepting chemotaxis
222175
223602
1
1428




receptor/sensory transducer


TM.orf0215
dadA
D-amino acid dehydrogenase small subunit
224981
223725
−2
1257


TM.orf0216
dadB
putative alanine racemase, catabolic
226230
225064
−1
1167


TM.orf0217

Leucine-responsive regulatory protein
226382
226843
3
462


TM.orf0218
linC
UcpA protein
226937
227698
3
762


TM.orf0219
ydeE
transcriptional regulatory protein
228561
227701
−1
861


TM.orf0220

conserved hypothetical protein
228712
229140
1
429


TM.orf0221
htr5
methyl-accepting chemotaxis protein
230871
229171
−1
1701


TM.orf0222
ydhC
drug resistance transporter, Bcr/CflA subfamily
232207
230993
−3
1215


TM.orf0223

short-chain dehydrogenase/reductase SDR
233060
232257
−2
804


TM.orf0224

2-oxoglutarate and iron-dependent oxygenase
233851
233171
−3
681




domain-containing protein 1


TM.orf0225
mmgC
putative acyl-CoA dehydrogenase
234531
235685
2
1155


TM.orf0226
lcfB
Acyl-CoA synthetase/AMP-acid ligase II
235682
237250
3
1569


TM.orf0227
sam
S-adenosylmethionine uptake transporter
238234
237323
−3
912


TM.orf0228
Cml2
possible acetyltransferase
238800
238231
−1
570


TM.orf0229

chemotaxis sensory transducer
238976
241060
3
2085


TM.orf0230
ileS
Isoleucyl-tRNA synthetase
241390
242553
1
1164


TM.orf0231
gcvA
transcriptional regulator
242561
243562
3
1002


TM.orf0232

GDSL-like lipase/acylhydrolase
244208
243579
−2
630


TM.orf0233
ntcA
cAMP-binding protein - catabolite protein
244888
244205
−3
684




activator and regulatory subunit of cAMP-




dependent protein kinase


TM.orf0234

conserved hypothetical protein
245056
245658
1
603


TM.orf0235

class II aldolase/adducin family protein
245663
246508
3
846


TM.orf0236

Carboxymethylenebutenolidase
246645
247532
2
888


TM.orf0237

Monofunctional biosynthetic peptidoglycan
248200
247553
−3
648




transglycosylase


TM.orf0238

Extracellular serine protease
248526
255200
2
6675


TM.orf0239

aromatic compounds catabolic protein
255761
255240
−2
522


TM.orf0240
mhqR
MarR family transcriptional regulator
255862
256290
1
429


TM.orf0241

putative lysine decarboxylase
256878
256297
−1
582


TM.orf0242
dctP
putative C4 dicarboxylate binding protein
257203
258192
1
990


TM.orf0243
siaT
Tripartite ATP-independent periplasmic
258225
258797
2
573




transporter DctQ component


TM.orf0244
siaT
TRAP dicarboxylate transporter, DctM subunit
258794
260089
3
1296


TM.orf0245

conserved hypothetical protein
260423
260172
−2
252


TM.orf0246
gcvA
LysR family transcriptional regulator
260662
261588
1
927


TM.orf0247

conserved hypothetical protein
261623
262540
3
918


TM.orf0248
cysA
sulfate ABC transporter, ATPase subunit
262652
263767
3
1116


TM.orf0249
yhaZ
conserved hypothetical protein
264557
263808
−2
750


TM.orf0250
yetL
regulatory protein, MarR
265045
264554
−3
492


TM.orf0251
bchO
ethyl ferulate-hydrolyzing esterase
266038
265088
−3
951


TM.orf0252

FAD dependent oxidoreductase
267555
266038
−1
1518


TM.orf0253

TetR family transcriptional regulator
267715
268386
1
672


TM.orf0254
ybaN
Inner membrane protein ybaN
268811
268383
−2
429


TM.orf0255
katA
catalase
270504
269020
−1
1485


TM.orf0256

2-polyprenyl-6-methoxyphenol hydroxylase
271822
270644
−3
1179


TM.orf0257

Major facilitator superfamily domain-containing
273069
271819
−1
1251




protein 3


TM.orf0258
fyuA
TonB-dependent receptor
275101
273074
−3
2028


TM.orf0259
pchR
transcriptional regulator, arac family
275300
276286
3
987


TM.orf0260

cation diffusion facilitator family transporter
277241
276246
−2
996


TM.orf0261
tcmP
O-methyltransferase domain-containing
278163
277291
−1
873




protein


TM.orf0262
oxyR
hydrogen peroxide-inducible genes activator
279196
278279
−3
918


TM.orf0263

hypothetical protein
279330
279716
2
387


TM.orf0264
proS
prolyl-tRNA synthetase
279842
281365
3
1524


TM.orf0265

conserved hypothetical protein
281575
282030
1
456


TM.orf0266

zinc-finger protein
282178
282603
1
426


TM.orf0267
ohrR
transcriptional regulator, MarR family
282856
283302
1
447


TM.orf0268
ydhC
transcriptional regulator
284050
283334
−3
717


TM.orf0269

class II aldolase/adducin family protein
284230
285009
1
780


TM.orf0270
gsiB
ABC transporter substrate binding protein
285156
286703
2
1548


TM.orf0271
appB
oligopeptide ABC transporter
286825
287802
1
978


TM.orf0272
appC
ABC transporter permease protein
287799
288638
2
840


TM.orf0273
gsiA
ABC transporter ATP-binding protein
288650
290305
3
1656


TM.orf0274
ghrA
D-isomer specific 2-hydroxyacid
290302
291273
1
972




dehydrogenase NAD-binding


TM.orf0275
aphA
histone deacetylase family protein
291313
292341
1
1029


TM.orf0276

aminotransferase
292397
293764
3
1368


TM.orf0277
exoT
putative succinoglycan transport protein
294288
295754
2
1467


TM.orf0278

conserved hypothetical protein
296735
297058
3
324


TM.orf0279

hypothetical protein
297314
297114
−2
201


TM.orf0280

conserved hypothetical protein
298793
298524
−2
270


TM.orf0281
lutR
transcriptional regulator-like
299671
298943
−3
729


TM.orf0282

TRAP dicarboxylate transporter, DctQ subunit
300331
299783
−3
549


TM.orf0283

C4-dicarboxylate transporter
301644
300337
−1
1308


TM.orf0284
dctB
possible TrapT family, dctP subunit, C4-
302889
301747
−1
1143




dicarboxylate periplasmic binding protein


TM.orf0285

aminoglycoside phosphotransferase
304023
302923
−1
1101


TM.orf0286
crt
enoyl-CoA hydratase
304211
305059
3
849


TM.orf0287
potI
binding-protein dependent transport system
306353
305532
−2
822




inner membrane protein


TM.orf0288
potH
binding-protein dependent transport system
307310
306372
−2
939




inner membrane protein


TM.orf0289
potG
putrescine transport ATP-binding protein PotG
308592
307378
−1
1215


TM.orf0290
potF
PotF
309829
308690
−3
1140


TM.orf0291
puuA
glutamine synthetase
311504
310074
−2
1431


TM.orf0292
dppF
putative oligopeptide ABC transporter (ATP
313018
311990
−3
1029




binding protein)


TM.orf0293
appD
oligopeptide/dipeptide ABC transporter,
314052
313015
−1
1038




ATPase subunit


TM.orf0294
dppC
Dipeptide transport system permease protein
315002
314124
−2
879




dppC


TM.orf0295

binding-protein-dependent transport systems
315975
315019
−1
957




inner membrane component


TM.orf0296
hbpA
extracellular solute-binding protein
317578
316088
−3
1491


TM.orf0297

peptidase S58 DmpA
318724
317585
−3
1140


TM.orf0298
glxR
2-hydroxy-3-oxopropionate reductase
319943
318963
−2
981


TM.orf0299

pyridoxamine 5′-phosphate oxidase family
320088
320702
2
615




protein


TM.orf0300

conserved hypothetical protein
321132
321632
2
501


TM.orf0301
proC
pyrroline-5-carboxylate reductase
321639
322481
2
843


TM.orf0302

conserved hypothetical protein
322663
323526
1
864


TM.orf0303

Carboxymethylenebutenolidase
324313
323603
−3
711


TM.orf0304

conserved hypothetical protein
324611
325759
3
1149


TM.orf0305

conserved hypothetical protein
325814
326329
3
516


TM.orf0306
engD
Predicted GTPase, probable translation factor
327522
326422
−1
1101


TM.orf0307
pth
peptidyl-tRNA hydrolase
328177
327527
−3
651


TM.orf0308
rplY
50S ribosomal protein L25/general stress
328797
328183
−1
615




protein Ctc


TM.orf0309
prs
ribose-phosphate pyrophosphokinase
329931
328942
−1
990


TM.orf0310

conserved hypothetical protein
330683
330189
−2
495


TM.orf0311
ade
adenine deaminase
332662
330908
−3
1755


TM.orf0312

conserved hypothetical protein
333599
332781
−2
819


TM.orf0313
midA
conserved hypothetical protein
334801
333623
−3
1179


TM.orf0314

Prolipoprotein diacylglyceryl transferase
335616
334798
−1
819


TM.orf0315

protein conserved in bacteria
335984
336340
3
357


TM.orf0316

putative membrane protein
336346
337263
1
918


TM.orf0317
sdpR
transcriptional regulator, ArsR family
337414
338223
1
810


TM.orf0318
rpmB
ribosomal protein L28
338503
338814
1
312


TM.orf0319

conserved hypothetical protein
339013
339561
1
549


TM.orf0320
hyfR
putative PAS/PAC sensor protein
341505
339562
−1
1944


TM.orf0321
nrtC
putative nitrate transporter component, nrtA
341772
343175
2
1404


TM.orf0322
nrtB
possible nitrate transport system permease
343200
344030
2
831




protein


TM.orf0323
cmpD
nitrate ABC transporter, ATPase subunits C
344049
344954
2
906




and D


TM.orf0364
ydhP
putative transmembrane efflux precursor
382165
383358
1
1194




protein


TM.orf0365

conserved hypothetical protein
383415
383909
2
495


TM.orf0366

conserved hypothetical protein
383926
385074
1
1149


TM.orf0367

52.8 kDa protein in TAR-I ttuC′ 3′region
386612
385080
−2
1533


TM.orf0368

tricarboxylic transport
387097
386630
−3
468


TM.orf0369
yflP
conserved hypothetical protein
388109
387102
−2
1008


TM.orf0370
tctD
two component transcriptional regulator,
388416
389090
2
675




winged helix family


TM.orf0371

hypothetical protein
388417
388253
−3
165


TM.orf0372

histidine kinase
389101
390489
1
1389


TM.orf0373

extracellular solute-binding protein family 1
390486
391586
2
1101


TM.orf0374
ubiG
3-demethylubiquinone-9 3-methyltransferase
392398
391607
−3
792


TM.orf0375
lysC
aspartate kinase
392580
393800
2
1221


TM.orf0376
ptsP
Signal transduction protein containing GAF
393993
396263
2
2271




and PtsI domains


TM.orf0377

conserved hypothetical protein
396535
397770
1
1236


TM.orf0378
ispG
4-hydroxy-3-methylbut-2-en-1-yl diphosphate
397921
399060
1
1140




synthase


TM.orf0379
hisS
Histidyl-tRNA synthetase, class IIa
399126
400370
2
1245


TM.orf0380
prfA
peptide chain release factor RF-1
400381
401469
1
1089


TM.orf0381
hemK
HemK family modification methylase
401466
402362
2
897


TM.orf0382

conserved hypothetical protein
402540
403709
2
1170


TM.orf0383
mal
MOSC domain containing protein
404581
403811
−3
771


TM.orf0384
clpB
ATP-dependent Clp protease ATP-binding
404822
407419
3
2598




subunit


TM.orf0385
yebA
Peptidase M23B
408960
407533
−1
1428


TM.orf0386

outer membrane autotransporter barrel
415506
418865
2
3360




domain-containing protein


TM.orf0387
glpR
glycerol-3-phosphate transcriptional regulator
418973
419752
3
780




protein


TM.orf0388
mco
Multicopper oxidase family
421157
419772
−2
1386


TM.orf0389
pchR
PchR
421349
422161
3
813


TM.orf0390
fhuA
outer membrane ferripyochelin receptor
422306
424453
3
2148


TM.orf0391

membrane protein
424458
425720
2
1263


TM.orf0392

PepSY-associated TM helix domain protein
425720
426814
3
1095


TM.orf0393

(Acyl-carrier protein) phosphodiesterase
427469
426789
−2
681


TM.orf0394
gcvA
LysR family transcriptional regulator
427576
428472
1
897


TM.orf0395
yvbU
LysR family transcriptional regulator
428469
429377
2
909


TM.orf0396

conserved hypothetical protein
429458
430402
3
945


TM.orf0397
ordL
FAD dependent oxidoreductase
430538
431851
3
1314


TM.orf0398

TRAP transporter, 4TM/12TM fusion protein
433867
431906
−3
1962


TM.orf0399

TRAP transporter solute receptor TAXI family
435070
434003
−3
1068




protein


TM.orf0400
ybiO
MscS Mechanosensitive ion channel
435428
438028
3
2601


TM.orf0401
yadH
Inner membrane transport permease yadH
438183
438971
2
789


TM.orf0402

conserved hypothetical protein
439111
439530
1
420


TM.orf0403
ftsK
cell divisionFtsK/SpoIIIE
439527
441980
2
2454


TM.orf0404

Glutathione peroxidase
442138
442779
1
642


TM.orf0405

conserved hypothetical protein
443279
442806
−2
474


TM.orf0406

histone deacetylase
444178
443276
−3
903


TM.orf0407

Outer membrane protein and related
444712
445557
1
846




peptidoglycan-associated lipo protein


TM.orf0408
asmA
AsmA protein
445810
447792
1
1983


TM.orf0409

conserved hypothetical protein
447945
448235
2
291


TM.orf0410
hupC
cytochrome B561
448239
448796
2
558


TM.orf0411

Ion transport 2 domain protein
448999
449613
1
615


TM.orf0412
yajO
putative Oxidoreductase
450653
449628
−2
1026


TM.orf0413

protein tyrosine phosphatase
451254
450781
−1
474


TM.orf0414
ppc
phosphoenolpyruvate carboxylase
451412
454207
3
2796


TM.orf0415
dgdR
transcriptional regulator, LysR family
454237
455127
1
891


TM.orf0416
ndhF
putative NADH-quinone oxidoreductase
455220
456689
2
1470




subunit 5


TM.orf0417

conserved hypothetical protein
456682
459156
1
2475


TM.orf0418

nitrogen regulatory protein P-II
459203
459559
3
357


TM.orf0419
regA
transcriptional regulator
460693
459683
−3
1011


TM.orf0420
fruB
phosphotransferase system, enzyme I
460971
463514
2
2544


TM.orf0421
fruK
1-phosphofructokinase
463511
464491
3
981


TM.orf0422
fruA
PTS system, fructose-specific IIC component
464526
466307
2
1782


TM.orf0423
ywoC
Amidases related to nicotinamidase
467048
466344
−2
705


TM.orf0424
foxA
TonB-dependent siderophore receptor
467333
469402
3
2070


TM.orf0425
bioA
adenosylmethionine--8-amino-7-oxononanoate
471910
470576
−3
1335




transaminase


TM.orf0426
bioD
dithiobiotin synthetase
472545
471907
−1
639


TM.orf0427

8-amino-7-oxononanoate synthase BioF
473678
472542
−2
1137


TM.orf0428
bioB
biotin synthase
474574
473675
−3
900


TM.orf0429
ydhC
transcriptional regulatory protein
474814
475455
1
642


TM.orf0430
ydeP
oxidoreductase alpha (molybdopterin) subunit
475529
477859
3
2331


TM.orf0431
gno
dehydrogenase
478626
477841
−1
786


TM.orf0432

conserved hypothetical protein
478688
480127
3
1440


TM.orf0433
potA
ABC transporter related protein
480236
481363
3
1128


TM.orf0434

ABC transporter, periplasmic solute-binding
481456
482583
1
1128




protein


TM.orf0435
potB
binding-protein-dependent transport systems
482680
483660
1
981




inner membrane component


TM.orf0436
potC
binding-protein-dependent transport systems
483657
484469
2
813




inner membrane component


TM.orf0437

histidinol dehydrogenase
484499
485821
3
1323


TM.orf0438
ureR
transcriptional regulator, AraC family protein
486830
485865
−2
966


TM.orf0439

outer membrane protein W precursor
487875
487210
−1
666


TM.orf0440
hemN
oxygen-independent coproporphyrinogen III
488180
489589
3
1410




oxidase


TM.orf0441
dhlA
haloalkane dehalogenase
490541
489624
−2
918


TM.orf0442

arginine exporter protein ArgO
491285
490656
−2
630


TM.orf0443
glpD
glycerol-3-phosphate dehydrogenase
491733
493265
2
1533


TM.orf0444
glpK
glycerol kinase
493445
494989
3
1545


TM.orf0445

TPR repeat-containing protein
495112
495654
1
543


TM.orf0446

conserved hypothetical protein
495786
496427
2
642


TM.orf0447

hypothetical protein
496544
496660
3
117


TM.orf0448
kdpA
potassium-transporting ATPase subunit A
496804
498504
1
1701


TM.orf0449
kdpB
potassium-translocating P-type ATPase B
498519
500567
2
2049




subunit


TM.orf0450
kdpC
potassium-transporting ATPase subunit C
500585
501175
3
591


TM.orf0451
kdpD
two-component system, OmpR family, sensor
501204
503951
2
2748




histidine kinase


TM.orf0452
kdpE
two-component system, response regulator
503973
504704
2
732


TM.orf0453

conserved hypothetical protein
505112
504765
−2
348


TM.orf0454
nthB
nitrile hydratase beta subunit
505831
505157
−3
675


TM.orf0455
nthA
nitrile hydratase alpha subunit
506502
505828
−1
675


TM.orf0456
gcvA
transcriptional regulator
507496
506543
−3
954


TM.orf0457

hypothetical protein
508111
507761
−3
351


TM.orf0458

globin
508687
508247
−3
441


TM.orf0459

ErfK/YbiS/YcfS/YnhG
508807
509469
1
663


TM.orf0460

putative lipoprotein
509478
510302
2
825


TM.orf0461
arsC
protein tyrosine phosphatase
510431
510949
3
519


TM.orf0462
pdhR
GntR domain-containing protein
511748
510972
−2
777


TM.orf0463

FMN-dependent alpha-hydroxy acid
511872
513029
2
1158




dehydrogenase


TM.orf0464

isocitrate lyase and phosphorylmutase
513155
514018
3
864


TM.orf0465

hypothetical protein
514015
514371
1
357


TM.orf0466

ABC transporter substrate-binding protein
514469
515752
3
1284


TM.orf0467

conserved hypothetical protein
517165
516215
−3
951


TM.orf0468

peptidase
517201
517578
1
378


TM.orf0469
qseB
two-component transcriptional regulator FeuP,
517575
518240
2
666




winged helix family


TM.orf0470
phoQ
two-component sensor histidine kinase
518237
519682
3
1446


TM.orf0471
iorB
Twin-arginine translocation pathway signal
521914
519695
−3
2220


TM.orf0472
iorA
putative aldehyde dehydrogenase subunit III
522370
521918
−3
453


TM.orf0473

transcriptional regulator, AraC family
522591
523544
2
954


TM.orf0474
lcfA
putative ligase
525113
523548
−2
1566


TM.orf0475

Extracellular ligand-binding receptor
526400
525171
−2
1230


TM.orf0476
petP
transcriptional regulator
526590
527138
2
549


TM.orf0477

conserved hypothetical protein
527847
527146
−1
702


TM.orf0478
panC
pantoate--beta-alanine ligase
528817
527900
−3
918


TM.orf0479

hypothetical protein
529043
530308
3
1266


TM.orf0480

hypothetical protein
530716
530312
−3
405


TM.orf0481
ribH
6,7-dimethyl-8-ribityllumazine synthase
531291
530773
−1
519


TM.orf0482
gmhB
Histidinol phosphatase and related
531698
532249
3
552




phosphatases


TM.orf0483
paaG
enoyl-CoA hydratase
533046
532252
−1
795


TM.orf0484

radical SAM domain-containing protein
533444
534793
3
1350


TM.orf0485
bchE
Radical SAM domain protein
534908
536443
3
1536


TM.orf0486

AMP-dependent synthetase and ligase
536464
538260
1
1797


TM.orf0487
eryA
polyketide synthase
538257
545873
2
7617


TM.orf0488

thioesterase domain-containing protein
545926
546360
1
435


TM.orf0489
acpP
acyl carrier protein
546431
546682
3
252


TM.orf0490
fabF
3-oxoacyl-[acyl-carrier-protein] synthase II
546700
548034
1
1335




(Beta-ketoacyl-ACP synthase II) (KAS II)


TM.orf0491

phage tail Collar
548247
548765
2
519


TM.orf0492

Tail Collar domain protein
548785
549333
1
549


TM.orf0493

phage tail Collar
549347
549880
3
534


TM.orf0494

Nitrilase/cyanide hydratase and apolipoprotein
551667
549940
−1
1728




N-acyltransferase


TM.orf0495
braG
putative branched-chain amino acid ABC
552409
551714
−3
696




transporter, ATP-binding protein


TM.orf0496
braF
branched chain amino acid ABC transporter
553173
552409
−1
765




ATP-binding protein


TM.orf0497
IivM
putative branched-chain amino acid ABC
554282
553170
−2
1113




transporter, permease protein


TM.orf0498
braD
putative permease component of ABC
555185
554292
−2
894




transporter


TM.orf0499
amiC
Aliphatic amidase expression-regulating
556529
555276
−2
1254




protein


TM.orf0500
amiR
two component response regulator
557586
556978
−1
609


TM.orf0501
amiC
two component sensor kinase
558743
557613
−2
1131


TM.orf0502

major facilitator transporter
559050
560237
2
1188


TM.orf0503
exoD
exopolysaccharide synthesis, ExoD
560901
560248
−1
654


TM.orf0504

ABC-type transport system, periplasmic
561238
561855
1
618




component


TM.orf0505

membrane protein
561833
562960
3
1128


TM.orf0506
dokA
Signal transduction histidine kinase
563084
564592
3
1509


TM.orf0507

putative phosphohistidine phosphatase, SixA
564654
565271
2
618


TM.orf0508
crtK
TspO and MBR like protein
565318
565920
1
603


TM.orf0509
dhkA
PAS fold family
565990
567486
1
1497


TM.orf0510
GCDH
acyl-CoA dehydrogenase domain-containing
568693
567503
−3
1191




protein


TM.orf0511

nitroreductase
568851
569519
2
669


TM.orf0512

Leu/Ile/Val-binding protein
570792
569566
−1
1227


TM.orf0513
lcfB
AMP-dependent synthetase and ligase
571032
572663
2
1632


TM.orf0514
ybcL
putative efflux transporter
573873
572689
−1
1185


TM.orf0515
nahR
transcriptional regulator, LysR family
574012
574938
1
927


TM.orf0516
ybaK
ybaK/ebsC protein
575000
575491
3
492


TM.orf0517

Phospholipase/Carboxylesterase
576117
575497
−1
621


TM.orf0518

thioredoxin reductase
577220
576114
−2
1107


TM.orf0519
FMO5
monooxygenase
578828
577506
−2
1323


TM.orf0520

NAD-dependent epimerase/dehydratase
579816
578839
−1
978


TM.orf0521
acnR
putative TetR-family transcriptional regulator
579957
580628
2
672


TM.orf0522

acyl-CoA dehydrogenase domain protein
580669
581832
1
1164


TM.orf0523
yfdE
L-carnitine dehydratase/bile acid-inducible
581847
582971
2
1125




protein F


TM.orf0524
htd2
Hydroxyacyl-thioester dehydratase type 2
582968
583837
3
870


TM.orf0525
yciC
cobalamin synthesis protein P47K
583974
585140
2
1167




CH42_BURCC RecName: Full = GTP


TM.orf0526

cyclohydrolase folE2 2
585216
586118
2
903


TM.orf0527

carbonic anhydrase/acetyltransferase
586156
586716
1
561




isoleucine patch superfamily


TM.orf0528
thrS
threonyl-tRNA synthetase
586723
588711
1
1989


TM.orf0529
allB
dihydroorotase
588708
590063
2
1356


TM.orf0530
lmrA
regulatory protein, TetR
590631
590041
−1
591


TM.orf0531
pksS
cytochrome P450
590886
592121
2
1236


TM.orf0532

Enoyl-CoA hydratase/isomerase
592172
593083
3
912


TM.orf0533

alanyl-tRNA synthetase-like protein
593148
593792
2
645


TM.orf0534

hypothetical protein
593789
593926
3
138


TM.orf0535

metal dependent phosphohydrolase
594581
593940
−2
642


TM.orf0536

AraC family transcriptional regulator
594749
595756
3
1008


TM.orf0537
phnO
acetyltransferase protein
596183
595719
−2
465


TM.orf0538

thiolase
597626
596370
−2
1257


TM.orf0539
pab
para-aminobenzoate synthase
597796
599931
1
2136


TM.orf0540

conserved hypothetical protein
600061
600906
1
846


TM.orf0541
qor
alcohol dehydrogenase
602007
601015
−1
993


TM.orf0542
mauR
transcriptional regulator protein
602150
603052
3
903


TM.orf0543
catA
putative dioxygenase
603116
603706
3
591


TM.orf0544
yeaM
AraC family transcriptional regulator
604467
603676
−1
792


TM.orf0545

conserved hypothetical protein
604608
605018
2
411


TM.orf0546
iorA
indolepyruvate ferredoxin oxidoreductase
607828
605153
−3
2676




subunit alpha/beta


TM.orf0547
ADH4
putative iron-containing alcohol
609219
610397
2
1179




dehydrogenase


TM.orf0548
yncG
glutathione S-transferase domain-containing
610399
611100
1
702




protein


TM.orf0549

thioesterase family protein
611097
611534
2
438


TM.orf0550

conserved hypothetical protein
611534
611995
3
462


TM.orf0551
lcfB
putative AMP-dependent synthetase and
612106
613716
1
1611




ligase


TM.orf0552

chemotaxis transducer
613861
615543
1
1683


TM.orf0553

conserved hypothetical protein
616401
615544
−1
858


TM.orf0554
acoA
Thiamine pyrophosphate-dependent
616817
617848
3
1032




dehydrogenase, E1 component alpha subunit


TM.orf0555

putative pyruvate dehydrogenase E1 beta
617854
618828
1
975




subunit


TM.orf0556
yiaO
2,3-diketo-L-gulonate-binding periplasmic
618991
620031
1
1041




protein yiaO


TM.orf0557
yiaM
Tripartite ATP-independent periplasmic
620150
620704
3
555




transporter DctQ component


TM.orf0558

putative membrane permease
620762
622084
3
1323


TM.orf0559
ilvB
putative acetolactate synthase large subunit
622226
623998
3
1773


TM.orf0560

conserved hypothetical protein
624099
624686
2
588


TM.orf0561
prfC
peptide chain release factor 3
626404
624803
−3
1602


TM.orf0562

virulence-associated protein VapB-like protein
626680
626970
1
291


TM.orf0563

hydrolase-like protein protein of the
627843
627025
−1
819




alpha/beta-hydrolase fold family


TM.orf0564

conserved hypothetical protein
627993
628298
2
306


TM.orf0565
exoI
nuclease (SNase-like)
628791
628339
−1
453


TM.orf0566
katG
catalase/peroxidase HPI
634943
632781
−2
2163


TM.orf0567

cysteine desulfuration protein SufE
635136
635567
2
432


TM.orf0568

class II aldolase/adducin domain protein
635598
636389
2
792


TM.orf0569

OsmC family protein
636514
636900
1
387


TM.orf0570

Amidase
636948
638387
2
1440


TM.orf0571

conserved hypothetical protein
638855
640081
3
1227


TM.orf0572

hypothetical protein
640122
640562
2
441


TM.orf0573
paaA
phenylacetic acid degradation protein (similar
640657
641412
1
756




to paaA)


TM.orf0574
soj
chromosome partitioning protein
642261
643097
2
837


TM.orf0575

conserved hypothetical protein
643191
643934
2
744


TM.orf0576

Heat shock protein DnaJ-like protein
643951
644727
1
777


TM.orf0577
exoN
UTP--glucose-1-phosphate uridylyltransferase
645077
645976
3
900


TM.orf0578
rkpK
putative UDP glucose dehydrogenase
646078
647397
1
1320


TM.orf0579
exoC
phosphomannomutase/phosphoglucomutase
647463
648872
2
1410


TM.orf0580
yjbE
putative transmembrane protein
649638
648958
−1
681


TM.orf0581

putative methyl-accepting chemotaxis protein
649939
651363
1
1425


TM.orf0582

ribokinase
652335
651364
−1
972


TM.orf0583

hypothetical protein
652572
652417
−1
156


TM.orf0584
dacF
D-alanyl-D-alanine carboxypeptidase
654331
652757
−3
1575


TM.orf0585

conserved hypothetical protein
654712
655467
1
756


TM.orf0586

conserved hypothetical protein
655702
656541
1
840


TM.orf0587
clpS
ATP-dependent Clp protease adaptor protein
656820
657164
2
345




ClpS


TM.orf0588

ATP-dependent Clp protease ATP-binding
657164
659569
3
2406




subunit ClpA


TM.orf0589

conserved hypothetical protein
661060
660392
−3
669


TM.orf0590

hypothetical protein
661403
661134
−2
270


TM.orf0591

hypothetical protein
661698
661420
−1
279


TM.orf0592

conserved hypothetical protein
662968
661796
−3
1173


TM.orf0593

conserved hypothetical protein
663406
663008
−3
399


TM.orf0594

conserved hypothetical protein
663738
663403
−1
336


TM.orf0595
dhbF
syringomycin synthetase
667650
663781
−1
3870


TM.orf0596

conserved hypothetical protein
668332
667784
−3
549


TM.orf0597

Chain A, Crystal Structure Of Cmls, A Flavin-
670077
668353
−1
1725




Dependent Halogenase


TM.orf0598

putative acetyltransferase
670754
670074
−2
681


TM.orf0599
lcfB
Long-chain-fatty-acid--CoA ligase
672127
670751
−3
1377


TM.orf0600
fabG
short-chain dehydrogenase/reductase SDR
672963
672124
−1
840


TM.orf0601
htpX
putative protease htpX homolog
674367
673189
−1
1179


TM.orf0602

conserved hypothetical protein
675506
674370
−2
1137


TM.orf0603
ilvI
acetolactate synthase 2 catalytic subunit
677353
675629
−3
1725


TM.orf0604

translation initiation inhibitor
677895
677428
−1
468


TM.orf0605
dhaS
betaine-aldehyde dehydrogenase
679419
677941
−1
1479


TM.orf0606
nemA
GTN reductase
680812
679697
−3
1116


TM.orf0607
lrp
leucine-responsive regulatory protein
681490
681008
−3
483


TM.orf0608
putA
delta-1-pyrroline-5-carboxylate dehydrogenase
681671
684853
3
3183


TM.orf0609
metA
Homoserine O-succinyltransferase
685094
686035
3
942


TM.orf0610
dhaR
transcriptional regulator, TetR family
686626
686042
−3
585


TM.orf0611
ybfB
major facilitator superfamily MFS-1
686797
688068
1
1272


TM.orf0612
PRX1
BcpB protein
688671
688087
−1
585


TM.orf0613

amino acid transporter LysE
689327
688668
−2
660


TM.orf0614

conserved hypothetical protein
690148
689324
−3
825


TM.orf0615
cmoA
methyltransferase domain protein
690891
690145
−1
747


TM.orf0616
ydeG
MFS-type transporter ydeG
692297
691077
−2
1221


TM.orf0617

inosine-uridine preferring nucleoside hydrolase
692498
693520
3
1023




family protein


TM.orf0618
hlyB
putative chemotaxis protein
693705
695978
2
2274


TM.orf0619

hypothetical protein
696163
696546
1
384


TM.orf0620
bfr
bacterioferritin
697119
696637
−1
483


TM.orf0621

hypothetical protein
697540
697331
−3
210


TM.orf0622

acetyl-CoA synthetase
699297
697648
−1
1650


TM.orf0623
ydbL
conserved hypothetical protein
699801
699409
−1
393


TM.orf0624
ynbE
conserved hypothetical protein
699995
699801
−2
195


TM.orf0625

conserved hypothetical protein
702253
700028
−3
2226


TM.orf0626
prtG
calcium binding hemolysin protein
704409
702337
−1
2073


TM.orf0627
cya
calcium binding hemolysin protein
707645
704406
−2
3240


TM.orf0628
hbd
3-hydroxybutyryl-CoA dehydrogenase
708626
707766
−2
861


TM.orf0629

acyl-CoA dehydrogenase family protein
710498
708687
−2
1812


TM.orf0630
yngJ
acyl-CoA dehydrogenase
711807
710659
−1
1149


TM.orf0631
yngJ
pimeloyl-CoA dehydrogenase, large subunit
713033
711837
−2
1197


TM.orf0632

peroxisomal bifunctional enzyme
715404
713272
−1
2133


TM.orf0633
IivF
ABC transporter related protein
716251
715547
−3
705


TM.orf0634
braF
ABC transporter related protein
717000
716248
−1
753


TM.orf0635
IivM
inner-membrane translocator
718319
717000
−2
1320


TM.orf0636
IivH
inner-membrane translocator
719266
718316
−3
951


TM.orf0637

ABC transporter, periplasmic branched chain
720794
719529
−2
1266




amino acid binding protein


TM.orf0638

enoyl-CoA hydratase
721169
721630
3
462


TM.orf0639
alkK
Acyl-CoA synthetases (AMP-forming)/AMP-
723334
721679
−3
1656




acid ligase II


TM.orf0640

stationary-phase survival protein SurE
724368
723562
−1
807


TM.orf0641
cpnA
dehydrogenase
724537
725313
1
777


TM.orf0642

short-chain dehydrogenase/reductase SDR
725393
726169
3
777


TM.orf0643

aminoglycoside phosphotransferase
726257
727384
3
1128


TM.orf0644

conserved hypothetical protein
729404
727473
−2
1932


TM.orf0645

Helix-turn-helix motif
730001
729531
−2
471


TM.orf0646

putative acyl-CoA dehydrogenase
731374
730163
−3
1212


TM.orf0647
acnR
TetR family transcriptional regulator
731751
732479
2
729


TM.orf0648

oxidoreductase, zinc-binding dehydrogenase
732536
733510
3
975




family


TM.orf0649

methyl-accepting chemotaxis sensory
733792
735945
1
2154




transducer


TM.orf0650

conserved hypothetical protein
736107
736688
2
582


TM.orf0651
cutL
carbon-monoxide dehydrogenase (acceptor)
739092
736771
−1
2322


TM.orf0652

chromate transporter
739344
740543
2
1200


TM.orf0653

transcriptional regulatory protein ZraR
741144
740554
−1
591


TM.orf0654
rpoH
RNA polymerase sigma-32 factor
741640
742587
1
948


TM.orf0655

soluble lytic murein transglycosylase and
743595
742660
−1
936




related regulatory protein


TM.orf0656

hypothetical protein
744009
743890
−1
120


TM.orf0657
phhA
phenylalanine 4-monooxygenase
745351
744503
−3
849


TM.orf0658
pat
GCN5-related N-acetyltransferase
746049
745474
−1
576


TM.orf0659

N-formylglutamate amidohydrolase
747143
746187
−2
957


TM.orf0660

conserved hypothetical protein
747698
748045
3
348


TM.orf0661

conserved hypothetical protein
748108
748539
1
432


TM.orf0662
ybiC
malate dehydrogenase
749609
748530
−2
1080


TM.orf0663

HTH-type transcriptional regulator in instable
749726
750433
3
708




DNA locus


TM.orf0664
dctP
c4-dicarboxylate-binding periplasmic protein
750531
751577
2
1047


TM.orf0665

Tripartite ATP-independent periplasmic
751608
752219
2
612




transporter DctQ component


TM.orf0666
siaT
C4-dicarboxylate TRAP-T family tripartite ATP-
752216
753484
3
1269




independent periplasmic transporter,




membrane protein, large subunit


TM.orf0667

conserved hypothetical protein
754396
753521
−3
876


TM.orf0668

hypothetical protein
754660
755205
1
546


TM.orf0669

sodium/hydrogen exchanger family protein
755322
757847
2
2526


TM.orf0670

conserved hypothetical protein
758235
757852
−1
384


TM.orf0671

conserved hypothetical protein
758543
758232
−2
312


TM.orf0672
codA
cytosine deaminase
759953
758661
−2
1293


TM.orf0673
yufQ
inner-membrane translocator
760898
759957
−2
942


TM.orf0674
yufP
inner-membrane translocator
761966
760917
−2
1050


TM.orf0675
yufO
ABC transporter component
763503
761956
−1
1548


TM.orf0676
med
Transcriptional activator protein med
764695
763700
−3
996


TM.orf0677
ytxM
Putative esterase ytxM
765825
764917
−1
909


TM.orf0678
apl
dedA family protein
766513
765920
−3
594


TM.orf0679

Arylformamidase
766698
767585
2
888


TM.orf0680
hldE
rfaE bifunctional protein
769104
767590
−1
1515


TM.orf0681
gmhA
Phosphoheptose isomerase
769759
769145
−3
615


TM.orf0682

cupin 2 domain-containing protein
769946
770377
3
432


TM.orf0683
hldD
nucleoside-diphosphate-sugar epimerase
771374
770388
−2
987


TM.orf0684

hypothetical protein
771640
771888
1
249


TM.orf0685
gfcB
lipoprotein gfcB
772654
771977
−3
678


TM.orf0686

sulfate adenylyltransferase subunit 2 sulfate
772921
773724
1
804




adenylatetransferase sat ATP-sulfurylase




small subunit


TM.orf0687
tuf
sulfate adenylyltransferase, large subunit
773744
774697
3
954


TM.orf0688
cysC
NodQ bifunctional enzyme
774694
775674
1
981


TM.orf0689
lcfA
4-coumarate-CoA ligase
777276
775735
−1
1542


TM.orf0690

peroxisomal 2,4-dienoyl-CoA reductase
778127
777318
−2
810


TM.orf0691

thiolase
779269
778124
−3
1146


TM.orf0692

DUF35
779685
779266
−1
420


TM.orf0693

peroxisomal multifunctional enzyme type 2
780596
779682
−2
915


TM.orf0694

alpha-methylacyl-CoA racemase
781834
780686
−3
1149


TM.orf0695
slyA
transcriptional regulator, MarR family
782310
781861
−1
450


TM.orf0696
FOX2
peroxisomal multifunctional enzyme type 2
783339
782434
−1
906


TM.orf0697

acyl-CoA dehydrogenase
783507
784658
2
1152


TM.orf0698
lcfA
acyl-CoA synthase
784655
786238
3
1584


TM.orf0699

enoyl-CoA hydratase/isomerase
786235
787038
1
804


TM.orf0700
siaT
trap dicarboxylate transporter, dctm subunit
788453
787113
−2
1341


TM.orf0701

tripartite ATP-independent periplasmic
788963
788457
−2
507




transporter DctQ


TM.orf0702
dctB
Bacterial extracellular solute-binding protein,
790096
789038
−3
1059




family 7


TM.orf0703
cysW
Sulfate ABC transporter permease CysW
791154
790300
−1
855


TM.orf0704
cysT
sulfate transport system permease protein
792007
791159
−3
849


TM.orf0705
sbp
sulfate ABC transporter, periplasmic sulfate-
793059
792013
−1
1047




binding protein


TM.orf0706

MUM4 (MUCILAGE-MODIFIED 4); UDP-4-
794335
793313
−3
1023




keto-6-deoxy-glucose-3,5-epimerase/UDP-4-




keto-rhamnose-4-keto-reductase/UDP-L-




rhamnose synthase/UDP-glucose 4,6-




dehydratase/catalytic


TM.orf0707
hldD
NAD-dependent epimerase/dehydratase
795138
794335
−1
804


TM.orf0708
rfbP
undecaprenyl-phosphate galactose
795619
796302
1
684




phosphotransferase


TM.orf0709
capI
NAD-dependent epimerase/dehydratase
796462
797448
1
987


TM.orf0710
capL
UDP-N-acetyl-D-mannosaminuronate
797455
798807
1
1353




dehydrogenase


TM.orf0711
kpsD
polysaccharide biosynthesis/export protein
798962
801733
3
2772


TM.orf0712
gfcD
lipoprotein gfcD
801746
804310
3
2565


TM.orf0713
rfbF
glucose-1-phosphate cytidylyltransferase
804589
805395
1
807


TM.orf0714
rfbN
glycosyl transferase, group 2 family protein
805747
806772
1
1026


TM.orf0715
algA
GDP-mannose pyrophosphorylase EpsQ
806941
808362
1
1422


TM.orf0716

hypothetical protein
809191
810693
1
1503


TM.orf0717

transposase, IS4
811033
810656
−3
378


TM.orf0718

transposase
811329
811030
−1
300


TM.orf0719

transposase
812031
811762
−1
270


TM.orf0720

putative transposase number 1 of insertion
812834
812286
−2
549




sequence NGRIS-2b


TM.orf0721

nitroreductase
813882
814241
2
360


TM.orf0722

glycosyltransferase
814291
815379
1
1089


TM.orf0723

conserved hypothetical protein
815651
815971
3
321


TM.orf0724
phnA
putative polybetahydroxybutyrate synthesis
816051
816371
2
321




PhnA protein


TM.orf0725

conserved hypothetical protein
816451
817203
1
753


TM.orf0726

transcriptional regulator, TetR family protein
817819
817235
−3
585


TM.orf0727
yxaH
putative membrane protein
817946
819001
3
1056


TM.orf0728
bioY
putative biotin transporter bioY
819599
819009
−2
591


TM.orf0729
yqjI
transcriptional regulator, PadR-like family
819792
820448
2
657


TM.orf0730

conserved hypothetical protein
820445
820891
3
447


TM.orf0731
ynfM
Inner membrane transport protein ynfM
822157
820892
−3
1266


TM.orf0732
alsR
transcriptional regulator, LysR family
822266
823165
3
900


TM.orf0733

glyoxalase family protein
823242
823730
2
489


TM.orf0734
ybaA
conserved hypothetical protein
823730
824089
3
360


TM.orf0735
ttr
Putative acetyltransferase protein
824625
824080
−1
546


TM.orf0736

XRE family transcriptional regulator
825205
824636
−3
570


TM.orf0737
pecS
transcriptional regulator protein
825751
825248
−3
504


TM.orf0738
pecM
putative transmembrane protein
825858
826748
2
891


TM.orf0739
yfmJ
NADP-dependent dehydrogenase
826767
827801
2
1035


TM.orf0740
ywfM
transporter ywfM
828741
827788
−1
954


TM.orf0741

ornithine cyclodeaminase/mu-crystallin
829797
828844
−1
954


TM.orf0742

conserved hypothetical protein
830365
829883
−3
483


TM.orf0743
hosA
MarR family transcriptional regulator
830469
830942
2
474


TM.orf0744

conserved hypothetical protein
831065
832447
3
1383


TM.orf0745

arsenate reductase and related
832953
832576
−1
378


TM.orf0746

peptidase M14, carboxypeptidase A
834152
832953
−2
1200


TM.orf0747
metC
Cys/Met metabolism pyridoxal-phosphate-
834281
835465
3
1185




dependent enzymes


TM.orf0748
rhlE
DEAD/DEAH box helicase domain-containing
836034
837620
2
1587




protein


TM.orf0749
bdlA
Methyl-accepting chemotaxis protein
839419
837737
−3
1683


TM.orf0750

phenazine biosynthesis protein PhzF family
840341
839514
−2
828


TM.orf0751

Multidrug resistance protein B
841645
840386
−3
1260


TM.orf0752

peptidase C45, acyl-coenzyme A: 6-
842814
841633
−1
1182




aminopenicillanic acid acyl-transferase


TM.orf0753
lysX
S6 modification enzyme RimK
843734
842811
−2
924


TM.orf0754
norM
MATE efflux family protein
845343
843844
−1
1500


TM.orf0755

conserved hypothetical protein
845638
846540
1
903


TM.orf0756

conserved hypothetical protein
846561
847322
2
762


TM.orf0757

GGDEF domain containing protein
847396
848274
1
879


TM.orf0758

diguanylate cyclase with PAS/PAC sensor
848280
848630
2
351


TM.orf0759

hypothetical protein
848932
849105
1
174


TM.orf0760
leuA
2-isopropylmalate synthase
849191
850897
3
1707


TM.orf0761
mreB
rod shape-determining protein MreB and
851190
852230
2
1041




related proteins


TM.orf0762
mreC
Rod shape-determining protein MreC
852430
853335
1
906


TM.orf0763

Cell shape-determining protein
853352
853867
3
516


TM.orf0764
mrdA
penicillin binding protein 2
853953
855959
2
2007


TM.orf0765
mrdB
Bacterial cell division membrane protein
855956
857107
3
1152


TM.orf0766

hypothetical protein
857429
857650
3
222


TM.orf0767
pldC
phospholipase D/transphosphatidylase
859194
857668
−1
1527


TM.orf0768
yjaB
GCN5-related N-acetyltransferase
859390
859845
1
456


TM.orf0769
rutF
flavin reductase domain-containing protein
860411
859860
−2
552


TM.orf0770
yhgD
transcriptional regulator, TetR family
860595
861254
2
660


TM.orf0771
eutG
maleylacetate reductase
862448
861276
−2
1173


TM.orf0772

conserved hypothetical protein
862698
863018
2
321


TM.orf0773

Bbp2
863033
863611
3
579


TM.orf0774

hypothetical protein
863608
863973
1
366


TM.orf0775

C repressor protein
864710
863964
−2
747


TM.orf0776

conserved hypothetical protein
864876
865304
2
429


TM.orf0777

conserved hypothetical protein
865831
865328
−3
504


TM.orf0778

hypothetical protein
866147
865857
−2
291


TM.orf0779
exoI
putative nuclease
867100
866357
−3
744


TM.orf0780
thrS
threonyl-tRNA synthetase
867419
869302
3
1884


TM.orf0781

Outer membrane lipoprotein-like
869483
870010
3
528


TM.orf0782

conserved hypothetical protein
870055
870642
1
588


TM.orf0783

filamentation induced by cAMP protein Fic
870812
871078
3
267


TM.orf0784

hypothetical protein
871209
872570
2
1362


TM.orf0785

hypothetical protein
872707
873117
1
411


TM.orf0786
arsB
membrane anion transport protein
873236
874462
3
1227


TM.orf0787

conserved hypothetical protein
874551
876017
2
1467


TM.orf0788

transcriptional regulator
876446
876036
−2
411


TM.orf0789
cry2
Deoxyribodipyrimidine photolyase
876900
878381
2
1482


TM.orf0790
icc
metallophosphoesterase
878427
879224
2
798


TM.orf0791
glxA
transcriptional regulator, AraC family
880193
879255
−2
939


TM.orf0792

ThiJ/PfpI family protein
880283
880981
3
699


TM.orf0793

extracellular ligand-binding receptor
882269
880995
−2
1275


TM.orf0794

oxidoreductase, short chain
883289
882483
−2
807




dehydrogenase/reductase family


TM.orf0795

Short-chain dehydrogenase/reductase SDR
883557
884315
2
759


TM.orf0796

Rhomboid family protein
884613
885104
2
492


TM.orf0797

conserved hypothetical protein
885466
885122
−3
345


TM.orf0798

conserved hypothetical protein
885588
886211
2
624


TM.orf0799

acetyltransferase
886436
886909
3
474


TM.orf0800

possible periplasmic substrate-binding protein,
887349
888662
2
1314




ABC-type amino acid ABC transporter


TM.orf0801
braD
inner-membrane translocator
888777
889673
2
897


TM.orf0802

inner-membrane translocator
889688
890713
3
1026


TM.orf0803
IivG
ABC transporter related protein
890710
891459
1
750


TM.orf0804
IivF
ABC transporter related protein
891479
892138
3
660


TM.orf0805
lcfB
putative acyl coenzyme A synthetase, long-
892181
893356
3
1176




chain-fatty-acid--CoA ligase


TM.orf0806
istB
IstB domain protein ATP-binding protein
894205
893414
−3
792


TM.orf0807
istA
Integrase, catalytic region
895700
894192
−2
1509


TM.orf0808
intZ
site-specific recombinase, phage integrase
897748
895811
−3
1938




family


TM.orf0809

hypothetical protein
898105
897965
−3
141


TM.orf0810

YGGT family protein
898781
898464
−2
318


TM.orf0811

Na+/solute symporter (Ssf family)
900627
898861
−1
1767


TM.orf0812

membrane protein
900934
900641
−3
294


TM.orf0813

hypothetical protein
901151
901759
3
609


TM.orf0814
dnaQ
DNA polymerase III, epsilon subunit
901740
903149
2
1410


TM.orf0815

Predicted signal-transduction protein
903243
904697
2
1455




containing cAMP-binding and CBS domains


TM.orf0816
pldB
alpha/beta hydrolase fold protein
904706
905701
3
996


TM.orf0817

short-chain dehydrogenase/reductase SDR
906481
905702
−3
780


TM.orf0818
ybfI
AraC family transcriptional regulator
907367
906504
−2
864


TM.orf0819
caiE
phenylacetic acid degradation protein PaaY
907543
908148
1
606


TM.orf0820

putative ABC transporter ATP-binding protein
908308
909990
1
1683


TM.orf0821

virulence-associated protein D
910204
910410
1
207


TM.orf0822
mlaC
toluene tolerance transporter
911169
910477
−1
693


TM.orf0823

Surface lipoprotein
912117
911266
−1
852


TM.orf0824

transcriptional regulator
912352
913419
1
1068


TM.orf0825
metF
5,10-methylenetetrahydrofolate reductase
913416
914360
2
945


TM.orf0826
metH
5-methyltetrahydrofolate--homocysteine
914413
917922
1
3510




methyltransferase


TM.orf0827

NAD(P)(+) transhydrogenase (AB-specific)
918395
919534
3
1140


TM.orf0828

NAD(P)(+) transhydrogenase (AB-specific)
919531
919950
1
420


TM.orf0829
pntB
NAD(P) transhydrogenase, beta subunit
919961
921364
3
1404


TM.orf0830
tauD
putative alpha-ketoglutarate-dependent taurine
922702
921779
−3
924




dioxygenase


TM.orf0831

Transcriptional regulator, AraC family protein
922852
923916
1
1065


TM.orf0832

conserved hypothetical protein
924188
923874
−2
315


TM.orf0833

30S ribosomal protein S21
924597
924391
−1
207


TM.orf0834

transcriptional activator, TenA family
924910
925581
1
672


TM.orf0835
COQ9
Ubiquinone biosynthesis protein
925664
926437
3
774


TM.orf0836
purK
phosphoribosylaminoimidazole carboxylase
927569
926454
−2
1116




ATPase subunit


TM.orf0837
purE
phosphoribosylcarboxyaminoimidazole mutase
928135
927611
−3
525


TM.orf0838

conserved hypothetical protein
928319
928906
3
588


TM.orf0839

sulfatase
929009
930739
3
1731


TM.orf0840

2-nitropropane dioxygenase
930795
931772
2
978


TM.orf0841

putative transcriptional regulator family
931811
932302
3
492


TM.orf0842

conserved hypothetical protein
932637
932819
2
183


TM.orf0843
ubiX
aromatic acid decarboxylase
933975
933316
−1
660


TM.orf0844

conserved hypothetical protein
934076
934243
3
168


TM.orf0845
alkK
Acyl-CoA synthetase (AMP-forming)/AMP-acid
936065
934428
−2
1638




ligase II


TM.orf0846
acsA
AMP-dependent synthetase and ligase
938073
936157
−1
1917


TM.orf0847

hypothetical protein
938444
938578
3
135


TM.orf0848
ybfB
MFS-type transporter ybfB
938618
939883
3
1266


TM.orf0849

AAA ATPase
939969
942092
2
2124


TM.orf0850

conserved hypothetical protein
942089
943507
3
1419


TM.orf0851

conserved hypothetical protein
944176
943583
−3
594


TM.orf0852

quinone oxidoreductase
945380
944355
−2
1026


TM.orf0853
rnz
beta-lactamase superfamily hydrolase
946345
945536
−3
810


TM.orf0854
rnz
beta-lactamase domain protein
946664
947518
3
855


TM.orf0855

cyclase family protein
948746
947757
−2
990


TM.orf0856

3-hydroxybutyrate dehydrogenase
949225
950004
1
780


TM.orf0857

alpha/beta hydrolase fold
950054
950875
3
822


TM.orf0858

putative excisonase protein
950962
951405
1
444


TM.orf0859
rutR
TetR family transcriptional regulator
952074
951400
−1
675


TM.orf0860
yufQ
inner-membrane translocator
953023
952079
−3
945


TM.orf0861
yufP
permease protein of sugar ABC transporter
954108
953020
−1
1089


TM.orf0862
yufO
ABC transporter, ATP-binding protein
955661
954105
−2
1557


TM.orf0863
med
Basic membrane lipoprotein
956701
955721
−3
981


TM.orf0864

putative iron/ascorbate oxidoreductase
956986
958041
1
1056


TM.orf0865

sulfite reductase
959499
958093
−1
1407


TM.orf0866

hypothetical protein
959937
959536
−1
402


TM.orf0867

putative cache sensor protein
960487
959996
−3
492


TM.orf0868

glycine betaine/proline transport system
961483
960599
−3
885




substrate-binding protein


TM.orf0869

binding-protein-dependent transport systems
962426
961581
−2
846




inner membrane component


TM.orf0870
proV
glycine betaine/L-proline ABC transporter,
963712
962426
−3
1287




ATPase subunit


TM.orf0871

putative addiction module antidote protein,
964193
964423
3
231




CopG/Arc/MetJ family


TM.orf0872
hspB
small heat shock protein
965045
964605
−2
441


TM.orf0873
ybfL
transposase, is4 family
965331
966446
2
1116


TM.orf0874
rpmE
large subunit ribosomal protein L31
966850
966629
−3
222


TM.orf0875
ytxE
OmpA/MotB domain protein
968218
966944
−3
1275


TM.orf0876

conserved hypothetical protein
969468
968293
−1
1176


TM.orf0877

hypothetical protein
969997
969587
−3
411


TM.orf0878
suhB
fructose-1 6-bisphosphatase
971114
970272
−2
843


TM.orf0879
efp
elongation factor P
971848
971282
−3
567


TM.orf0880
thiE
thiamine-phosphate pyrophosphorylase
972731
972063
−2
669


TM.orf0881
fbaB
fructose-bisphosphate aldolase
973648
972728
−3
921


TM.orf0882

hypothetical protein
974192
973854
−2
339


TM.orf0883
pgk
phosphoglycerate kinase
975422
974226
−2
1197


TM.orf0884
gap
glyceraldehyde-3-phosphate dehydrogenase
976536
975532
−1
1005


TM.orf0885

hypothetical protein
976579
976710
1
132


TM.orf0886
cbbT
transketolase
978670
976667
−3
2004


TM.orf0887
glnT
putative amino acid carrier
979078
980508
1
1431




protein(sodium/alanine symporter)


TM.orf0888
aspA
aspartate ammonia-lyase
980586
981959
2
1374


TM.orf0889
ansA
cytoplasmic asparaginase I
982881
982036
−1
846


TM.orf0890

Aspartate racemase
983627
982881
−2
747


TM.orf0891

putative transcriptional regulator
983797
984603
1
807


TM.orf0892

hypothetical protein
984949
985254
1
306


TM.orf0893

conserved hypothetical protein
985283
985633
3
351


TM.orf0894

5-formyltetrahydrofolate cyclo-ligase
985909
986610
1
702


TM.orf0895
ymdB
metallophosphoesterase
986721
987059
2
339


TM.orf0896
ymdB
conserved hypothetical protein
987053
987598
3
546


TM.orf0897

conserved hypothetical protein
987733
988482
1
750


TM.orf0898
ruvC
crossover junction endodeoxyribonuclease
988521
989021
2
501


TM.orf0899
ruvA
Holliday junction ATP-dependent DNA
989054
989701
3
648




helicase


TM.orf0900
ruvB
Holliday junction DNA helicase B
989698
990759
1
1062


TM.orf0901

tol-pal system-associated acyl-CoA
990862
991296
1
435




thioesterase


TM.orf0902
gatA
Glutamyl-tRNA(Gln) amidotransferase
992736
991339
−1
1398




subunit A


TM.orf0903
tolQ
biopolymer transport protein
993294
994028
2
735


TM.orf0904
tolR
biopolymer transport protein
994032
994490
2
459


TM.orf0905

hypothetical protein
995062
994682
−3
381


TM.orf0906

TolA protein
995410
995754
1
345


TM.orf0907
tolB
translocation protein TolB
995751
997130
2
1380


TM.orf0908

Outer membrane protein and related
997284
997796
2
513




peptidoglycan-associated lipo protein


TM.orf0909

tol-pal system protein YbgF
998013
999008
2
996


TM.orf0910
tilS
putative PP-loop superfamily ATPase
999013
1000494
1
1482


TM.orf0911
ftsH
cell division protease
1000491
1002434
2
1944


TM.orf0912
folP
dihydropteroate synthase
1002461
1003666
3
1206


TM.orf0913
glmM
phosphoglucosamine mutase
1003826
1005181
3
1356


TM.orf0914
thiD
phosphomethylpyrimidine kinase
1005334
1006173
1
840


TM.orf0915

phosphomethylpyrimidine kinase
1006170
1007015
2
846


TM.orf0916

isomerase
1007384
1008172
3
789


TM.orf0917

GGDEF domain-containing protein
1009326
1008169
−1
1158


TM.orf0918
serC
phosphoserine aminotransferase
1009580
1010746
3
1167


TM.orf0919
serA
D-3-phosphoglycerate dehydrogenase
1010864
1012441
3
1578


TM.orf0920
hisZ
ATP phosphoribosyltransferase regulatory
1012760
1013935
3
1176




subunit


TM.orf0921
purA
adenylosuccinate synthetase
1013949
1015232
2
1284


TM.orf0922

conserved hypothetical protein
1015398
1017425
2
2028


TM.orf0923

conserved hypothetical protein
1017441
1017908
2
468


TM.orf0924
IivF
branched-chain amino acid ABC transporter,
1018685
1017936
−2
750




ATP-binding protein


TM.orf0925
braF
branched-chain amino acid ABC transporter,
1019443
1018685
−3
759




ATP-binding protein


TM.orf0926
braE
inner-membrane translocator
1020607
1019456
−3
1152


TM.orf0927
IivH
inner-membrane translocator
1021634
1020609
−2
1026


TM.orf0928

ABC-type branched-chain amino acid
1023101
1021761
−2
1341




transporter


TM.orf0929

transcriptional regulator, XRE family protein
1023300
1024766
2
1467


TM.orf0930
arlR
protein containing response regulator domain,
1024837
1025208
1
372




but no DNA binding domain


TM.orf0931

hypothetical protein
1025230
1025514
1
285


TM.orf0932

Na+/solute symporter
1025519
1028239
3
2721


TM.orf0933

transcriptional regulator MazE
1028523
1028293
−1
231


TM.orf0934
rpoH
RNA polymerase factor sigma-32
1029554
1028631
−2
924


TM.orf0935
rluD
Pseudouridylate synthases, 23S RNA-specific
1031385
1030168
−1
1218


TM.orf0936

hypothetical protein
1031422
1031904
1
483


TM.orf0937

Mov34/MPN/PAD-1
1031901
1032383
2
483


TM.orf0938

(Uracil-5)-methyltransferase
1032463
1034070
1
1608


TM.orf0939
ltaE
putative low specificity L-threonine aldolase
1034067
1035119
2
1053


TM.orf0940
lcfB
malonyl-CoA synthase
1035551
1037068
3
1518


TM.orf0941
ywbO
thiol oxidoreductase FrnE
1037195
1037962
3
768


TM.orf0942
mdcF
malonate transporter
1038916
1037981
−3
936


TM.orf0943

N-acetyl-gamma-glutamyl-phosphate
1039420
1040418
1
999




reductase


TM.orf0944
puuR
HTH-type transcriptional regulator puuR
1041265
1040717
−3
549


TM.orf0945

omega-amino acid-pyruvate aminotransferase
1041612
1042934
2
1323


TM.orf0946
ald
alanine dehydrogenase
1043080
1044204
1
1125


TM.orf0947

addiction module antitoxin, RelB/DinJ family
1044340
1044486
1
147


TM.orf0948

putative multidrug-efflux transporter
1045981
1044515
−3
1467


TM.orf0949
pleC
PAS/PAC sensor signal transduction histidine
1046185
1047351
1
1167




kinase


TM.orf0950
dadA
glycine/D-amino acid oxidase
1048626
1047364
−1
1263


TM.orf0951
ygdD
inner membrane protein ygdD
1048788
1049189
2
402


TM.orf0952

hypothetical protein
1049217
1049336
2
120


TM.orf0953

AAA ATPase central domain protein
1049427
1050308
2
882


TM.orf0954

conserved hypothetical protein
1050366
1051544
2
1179


TM.orf0955

hypothetical protein
1051833
1052648
2
816


TM.orf0956
macB
ABC transporter related protein
1054578
1052626
−1
1953


TM.orf0957
macA
RND family efflux transporter MFP subunit
1055783
1054575
−2
1209


TM.orf0958

conserved hypothetical protein
1055843
1056796
3
954


TM.orf0959

membrane protein
1056813
1058180
2
1368


TM.orf0960
aprE
HlyD family secretion protein
1059543
1058170
−1
1374


TM.orf0961
aprD
ABC transporter, ATP-binding protein
1061372
1059540
−2
1833


TM.orf0962
yhjN
Putative ammonia monooxygenase
1062612
1061521
−1
1092


TM.orf0963

histone deacetylase-like amidohydrolase
1062890
1063828
3
939


TM.orf0964
xseB
Exonuclease VII small subunit
1063936
1064193
1
258


TM.orf0965
ispA
geranyltranstransferase
1064250
1065176
2
927


TM.orf0966
dxs
deoxyxylulose-5-phosphate synthase
1065466
1067403
1
1938


TM.orf0967
yqxC
Predicted rRNA methylase
1067420
1068229
3
810


TM.orf0968
fnr
transcriptional regulator, Crp
1068283
1068984
1
702


TM.orf0969
phbC
PHB de-polymerase
1070115
1068994
−1
1122


TM.orf0970

conserved hypothetical protein
1070686
1070255
−3
432


TM.orf0971

rhodanese-related sulfurtransferase
1070835
1071209
2
375


TM.orf0972

hypothetical protein
1071363
1071199
−1
165


TM.orf0973
lcfB
putative long-chain-fatty-acid CoA ligase
1072995
1071436
−1
1560


TM.orf0974
fadR
TetR family transcriptional regulator
1073737
1073117
−3
621


TM.orf0975
SCP2
lipid-transfer protein
1074051
1075232
2
1182


TM.orf0976

conserved hypothetical protein
1075263
1075730
2
468


TM.orf0977
FOX2
MaoC-like dehydratase
1075727
1076113
3
387


TM.orf0978

short-chain dehydrogenase/reductase SDR
1076176
1077003
1
828


TM.orf0979

acyl-CoA dehydrogenase domain protein
1077080
1078240
3
1161


TM.orf0980

conserved hypothetical protein
1078598
1078299
−2
300


TM.orf0981

putative epimerase PhzC/PhzF-like protein
1079828
1078869
−2
960


TM.orf0982
ynjA
conserved hypothetical protein
1080524
1079934
−2
591


TM.orf0983
aroC
chorismate synthase
1081676
1080603
−2
1074


TM.orf0984

short-chain dehydrogenase/reductase SDR
1082535
1081723
−1
813


TM.orf0985
yfkH
YihY family protein
1083690
1082737
−1
954


TM.orf0986

curved DNA-binding protein
1084583
1083687
−2
897


TM.orf0987
omp
17 kDa surface antigen precursor
1085187
1084711
−1
477


TM.orf0988
pdxH
pyridoxamine 5′-phosphate oxidase
1085540
1086133
3
594


TM.orf0989
fieF
cation efflux protein
1086130
1087056
1
927


TM.orf0990
apt
adenine phosphoribosyltransferase
1087198
1087722
1
525


TM.orf0991
tag
DNA-3-methyladenine glycosylase I
1088343
1087750
−1
594


TM.orf0992

Predicted aminomethyltransferase related to
1089265
1088336
−3
930




GcvT


TM.orf0993

glycosyl transferase family protein
1089430
1090422
1
993


TM.orf0994
ytcC
glycosyl transferase group 1
1090419
1091657
2
1239


TM.orf0995
lpsD
Glycosyltransferase
1091647
1092765
1
1119


TM.orf0996
asnB
asparagine synthase
1092798
1094612
2
1815


TM.orf0997
ypfG
conserved hypothetical protein
1095734
1094628
−2
1107


TM.orf0998
pleC
sensory transduction histidine kinase
1095958
1098360
1
2403


TM.orf0999
dcd
deoxycytidine triphosphate deaminase
1099261
1099815
1
555


TM.orf1000
murA
UDP-N-acetylglucosamine enolpyruvyl
1099904
1101193
3
1290




transferase


TM.orf1001
hisG
ATP phosphoribosyltransferase catalytic
1101245
1101928
3
684




subunit


TM.orf1002
hisD
histidinol dehydrogenase
1101912
1103213
2
1302


TM.orf1003

conserved hypothetical protein
1103327
1103848
3
522


TM.orf1004
arsC
low molecular weight phosphotyrosine protein
1103908
1104330
1
423




phosphatase


TM.orf1005
infA
translation initiation factor 1
1104528
1104746
2
219


TM.orf1006

maf protein
1104866
1105480
3
615


TM.orf1007
rng
ribonuclease G
1105477
1107108
1
1632


TM.orf1008

zinc-binding protein
1107142
1107381
1
240


TM.orf1009

hypothetical protein
1107832
1108107
1
276


TM.orf1010

hypothetical protein
1108278
1108138
−1
141


TM.orf1011

amidohydrolase family protein
1108294
1108461
1
168


TM.orf1012

conserved hypothetical protein
1108729
1109280
1
552


TM.orf1013
metG
Methionyl-tRNA synthetase
1110472
1109360
−3
1113


TM.orf1014

conserved hypothetical protein
1110999
1111595
2
597


TM.orf1015
metQ
lipoprotein, YaeC family
1112465
1111677
−2
789


TM.orf1016
cysD
O-acetylhomoserine/O-acetylserine
1114130
1112832
−2
1299




sulfhydrylase


TM.orf1017
leuA
pyruvate carboxyltransferase
1115574
1114237
−1
1338


TM.orf1018
oxyR
transcriptional regulator, LysR family
1115785
1116711
1
927


TM.orf1019
nuoE
NADH dehydrogenase (ubiquinone) 24 kDa
1116819
1117316
2
498




subunit


TM.orf1020

putative NAD-dependent formate
1117313
1118869
3
1557




dehydrogenase beta subunit protein


TM.orf1021
yjgC
formate dehydrogenase, alpha subunit
1118881
1121721
1
2841


TM.orf1022
fdhD
formate dehydrogenase accessory protein
1121738
1122535
3
798


TM.orf1023

putative NAD-dependent formate
1122552
1122764
2
213




dehydrogenase


TM.orf1024
nylB
6-aminohexanoate-dimer hydrolase
1123952
1122780
−2
1173


TM.orf1025
gsiA
ABC transporter ATP-binding protein
1125784
1123964
−3
1821


TM.orf1026
puuC
aldehyde dehydrogenase (acceptor)
1127313
1125781
−1
1533


TM.orf1027
soxB
Sarcosine oxidase subunit beta
1128720
1127365
−1
1356


TM.orf1028

acetyltransferase
1129144
1128707
−3
438


TM.orf1029

FAD dependent oxidoreductase
1129418
1130563
3
1146


TM.orf1030
appA
ABC superfamily ATP binding cassette
1130651
1132309
3
1659




transporter substrate-binding protein


TM.orf1031
appB
oligopeptide ABC superfamily ATP binding
1132328
1133278
3
951




cassette transporter, membrane protein


TM.orf1032
appC
ABC transporter permease
1133284
1134153
1
870


TM.orf1033
gcvA
transcriptional regulatory protein
1134178
1135080
1
903


TM.orf1034

major facilitator superfamily MFS_1
1136380
1135142
−3
1239


TM.orf1035
ywlC
Sua5/YciO/YrdC/YwlC family protein
1137113
1136391
−2
723


TM.orf1036

hypothetical protein
1137943
1137362
−3
582


TM.orf1037

hypothetical protein
1138346
1138122
−2
225


TM.orf1038

conserved hypothetical protein
1138468
1138971
1
504


TM.orf1039

hypothetical protein
1138968
1139462
2
495


TM.orf1040
ycfQ
transcriptional regulator, TetR family protein
1139539
1140165
1
627


TM.orf1041
fecI
RNA polymerase sigma-70 family protein
1140504
1141028
2
525


TM.orf1042

putative FecR
1141025
1141987
3
963


TM.orf1043
norA
oxidoreductase
1143149
1142049
−2
1101


TM.orf1044
ycaN
transcriptional regulator LysR family
1143262
1144149
1
888


TM.orf1045
foxA
TonB-dependent siderophore receptor
1144309
1146759
1
2451


TM.orf1046

PepSY-associated TM helix domain-containing
1146770
1147993
3
1224




protein


TM.orf1047
ntaB
Flavin reductase like domain protein
1148069
1148584
3
516


TM.orf1048

iron-sulfur cluster repair di-iron protein
1148678
1149136
3
459


TM.orf1049

signal peptide protein
1149672
1149154
−1
519


TM.orf1050

phosphoglycerate mutase family protein
1149860
1150432
3
573


TM.orf1051
ybfB
major facilitator superfamily MFS_1
1150696
1151955
1
1260


TM.orf1052

hypothetical protein
1151979
1152560
2
582


TM.orf1053
ubiG
3-demethylubiquinone-9 3-O-
1153440
1152544
−1
897




methyltransferase


TM.orf1054

peptidase M50
1154597
1153437
−2
1161


TM.orf1055

Lantibiotic dehydratase domain protein
1157020
1154594
−3
2427


TM.orf1056

hypothetical protein
1157871
1157017
−1
855


TM.orf1057

conserved hypothetical protein
1158788
1157871
−2
918


TM.orf1058
eamB
Lysine exporter protein (LYSE/YGGA)
1159483
1158854
−3
630


TM.orf1059

radical SAM domain-containing protein
1161690
1159567
−1
2124


TM.orf1060

hypothetical protein
1162116
1161901
−1
216


TM.orf1061

conserved hypothetical protein
1162425
1162712
2
288


TM.orf1062
yfkN
alkaline phosphatase
1162922
1167649
3
4728


TM.orf1063
prtC
Secreted protease C
1167664
1169685
1
2022


TM.orf1064

TolC family type I secretion outer membrane
1169792
1171222
3
1431




protein


TM.orf1065
paxB
putative exotoxin translocation ATP-binding
1171219
1173030
1
1812




protein PaxB


TM.orf1066
cyaD
type I secretion membrane fusion protein,
1173020
1174321
3
1302




HlyD family


TM.orf1067

conserved hypothetical protein
1175412
1174330
−1
1083


TM.orf1068
pleC
Signal transduction histidine kinase
1175791
1177161
1
1371


TM.orf1069
acoD
acetaldehyde dehydrogenase
1177358
1178905
3
1548


TM.orf1070

conserved hypothetical protein
1178941
1179459
1
519


TM.orf1071

conserved hypothetical protein
1179570
1180430
2
861


TM.orf1072

pyridoxamine 5′-phosphate oxidase-related
1180529
1180996
3
468




FMN-binding protein


TM.orf1073

conserved hypothetical protein
1181468
1180980
−2
489


TM.orf1074
fliS
flagellar protein FliS
1182138
1181662
−1
477


TM.orf1075
fliD
flagellar hook-associated protein 2 (FliD,
1184293
1182170
−3
2124




filament cap protein) precursor


TM.orf1076

flagellar protein FlaG
1184798
1184376
−2
423


TM.orf1077

flagellin
1186200
1185037
−1
1164


TM.orf1078

flagellin domain protein
1187778
1186615
−1
1164


TM.orf1079

alkylhydroperoxidase like protein, AhpD family
1188104
1188586
3
483


TM.orf1080

conserved hypothetical protein
1189248
1188577
−1
672


TM.orf1081

conserved hypothetical protein
1191698
1189254
−2
2445


TM.orf1082
celY
cellulase
1192993
1191689
−3
1305


TM.orf1083

cellulose synthase subunit B
1195779
1192990
−1
2790


TM.orf1084
bcsA
putative cellulose synthase catalytic subunit
1198133
1195797
−2
2337


TM.orf1085
fliB
Lysine-N-methylase
1199528
1198353
−2
1176


TM.orf1086
fliW
Flagellar assembly factor fliW
1200072
1199554
−1
519


TM.orf1087

flagellar hook-associated protein FlgL
1201094
1200192
−2
903


TM.orf1088
flgK
flagellar hook-associated protein
1202984
1201173
−2
1812


TM.orf1089

hypothetical protein
1203537
1203016
−1
522


TM.orf1090

chemotactic signal-response protein CheL
1203952
1203569
−3
384


TM.orf1091
flgI
flagellar P-ring protein precursor
1205127
1203964
−1
1164


TM.orf1092

flagellar assembly regulator FliX
1205467
1205895
1
429


TM.orf1093
dksA
DnaK suppressor protein
1206017
1206433
3
417


TM.orf1094
pleC
putative sensory box histidine kinase
1206708
1209452
2
2745


TM.orf1095

flagellar basal body L-ring protein
1210260
1209466
−1
795


TM.orf1096
flgA
flagellar basal body P-ring biosynthesis protein
1211316
1210324
−1
993




FlgA


TM.orf1097
flgG
flagellar basal-body rod protein FlgG
1212149
1211364
−2
786


TM.orf1098
flgF
flagellar basal-body rod protein
1212950
1212198
−2
753


TM.orf1099
fliL
flagellar basal body-associated protein
1213326
1213868
2
543


TM.orf1100
fliM
flagellar motor switch protein FliM
1213924
1215030
1
1107


TM.orf1101

ATPase involved in DNA repair
1215027
1215620
2
594


TM.orf1102

FlaA locus 229 kDa protein
1215704
1216519
3
816


TM.orf1103
cheY
Response regulator receiver
1216543
1216929
1
387


TM.orf1104

chemotaxis protein CheZ
1216979
1217641
3
663


TM.orf1105

PPE-repeat protein (cell mobility)
1217692
1218354
1
663


TM.orf1106
ytxE
chemotaxis MotB protein
1218351
1219100
2
750


TM.orf1107

conserved hypothetical protein
1219097
1222261
3
3165


TM.orf1108
rlmI
methyltransferase
1222402
1223379
1
978


TM.orf1109
ysgA
rRNA methylase
1223383
1224210
1
828


TM.orf1110

hypothetical protein
1224235
1224495
1
261


TM.orf1111
dhaR
TetR/AcrR family transcriptional regulator
1224589
1225215
1
627


TM.orf1112

hypothetical protein
1224617
1224492
−2
126


TM.orf1113
linC
2,5-dichloro-2,5-cyclohexadiene-1,4-
1225281
1226045
2
765




dioldehydrogenase


TM.orf1114

DMT family permease
1226948
1226052
−2
897


TM.orf1115
lrhA
LysR family transcriptional regulator
1227106
1227975
1
870


TM.orf1116
fliP
flagellar biosynthetic protein FliP
1229494
1228724
−3
771


TM.orf1117

hypothetical protein
1229949
1229491
−1
459


TM.orf1118

hypothetical protein
1230402
1230043
−1
360


TM.orf1119
ylqH
flagellar biosynthetic protein FlhB
1230732
1230448
−1
285


TM.orf1120
csrA
carbon storage regulator
1231119
1230856
−1
264


TM.orf1121
flgB
flagellar basal body rod protein FlgB
1231481
1231903
3
423


TM.orf1122
flgC
flagellar basal-body rod protein FlgC
1231952
1232359
3
408


TM.orf1123
fliE
flagellar hook-basal body complex protein FliE
1232420
1232734
3
315


TM.orf1124
yscS
flagellar biosynthetic protein FNQ
1232807
1233082
3
276


TM.orf1125
fliR
flagellar biosynthesis protein FliR
1233102
1233857
2
756


TM.orf1126
flhB
flagellar biosynthetic protein FlhB
1233875
1234954
3
1080


TM.orf1127

cell cycle histidine kinase CckA
1235020
1236945
1
1926


TM.orf1128

hypothetical protein
1237585
1237100
−3
486


TM.orf1129
recA
RecA protein
1237929
1239029
2
1101


TM.orf1130
alaS
alanyl-tRNA synthetase
1239345
1241999
2
2655


TM.orf1131

conserved hypothetical protein
1243214
1242069
−2
1146


TM.orf1132
rhsD
nematicidal protein 2
1248254
1243263
−2
4992


TM.orf1133

putative acetyltransferase
1249003
1248464
−3
540


TM.orf1134
yjiA
cobalamin synthesis protein CobW
1250103
1249000
−1
1104


TM.orf1135

3-hydroxyacyl-CoA dehydrogenase (hdb-1)
1250300
1251193
3
894


TM.orf1136
icd
isocitrate dehydrogenase
1252543
1251296
−3
1248


TM.orf1137

hypothetical protein
1252775
1253668
3
894


TM.orf1138
ygaU
protein containing LysM domain
1253779
1255026
1
1248


TM.orf1139
ywfA
MFS-type transporter ywfA
1256268
1255027
−1
1242


TM.orf1140

hypothetical protein
1259653
1259456
−3
198


TM.orf1141

hypothetical protein
1261070
1259721
−2
1350


TM.orf1142
yezE
TetR family transcriptional regulator
1261874
1261266
−2
609


TM.orf1143

conserved hypothetical protein
1261993
1262910
1
918


TM.orf1144
yfcG
glutathione S-transferase domain-containing
1262966
1263670
3
705




protein


TM.orf1145
yfcG
Glutathione S-transferase domain protein
1263679
1264389
1
711


TM.orf1146
rbsC
methyl-galactoside transport system permease
1265469
1264411
−1
1059




protein


TM.orf1147

ABC transporter ATP-binding protein
1266995
1265466
−2
1530


TM.orf1148
torT
ABC transporter substrate-binding protein
1268159
1267098
−2
1062


TM.orf1149
kipA
urea amidolyase related protein
1269275
1268304
−2
972


TM.orf1150

allophanate hydrolase subunit 1
1270140
1269265
−1
876


TM.orf1151
accC
biotin carboxylase
1271514
1270150
−1
1365


TM.orf1152

acetyl-CoA carboxylase biotin carboxyl carrier
1271758
1271522
−3
237




protein


TM.orf1153

conserved hypothetical protein
1272647
1271865
−2
783


TM.orf1154
cynR
transcriptional regulator
1272913
1273836
1
924


TM.orf1155

cupin superfamily protein
1274097
1275260
2
1164


TM.orf1156
pfpI
proteinase
1275836
1275285
−2
552


TM.orf1157

conserved hypothetical protein
1276516
1275911
−3
606


TM.orf1158
ynjF
phosphatidylglycerophosphate synthase
1276707
1277315
2
609


TM.orf1159

conserved hypothetical protein
1277454
1278356
2
903


TM.orf1160
ydjF
transcriptional regulator, DeoR family
1278360
1279133
2
774


TM.orf1161
ytfG
oxidoreductase ytfG
1280016
1279138
−1
879


TM.orf1162
ytcD
putative HTH-type transcriptional regulator
1280138
1280497
3
360


TM.orf1163
mdtC
AcrB
1283531
1280535
−2
2997


TM.orf1164

Membrane-fusion protein
1284902
1283676
−2
1227


TM.orf1165
padR
transcriptional regulator, PadR family protein
1285456
1284899
−3
558


TM.orf1166

dtdp-glucose 4,6-dehydratase
1286620
1285598
−3
1023


TM.orf1167

NAD-dependent epimerase/dehydratase
1287306
1286620
−1
687


TM.orf1168

methyltransferase, UbiE
1288319
1287462
−2
858


TM.orf1169
glmU
molybdopterin binding domain-containing
1290137
1288446
−2
1692




protein


TM.orf1170
pucA
xanthine dehydrogenase accessory factor
1290925
1290194
−3
732


TM.orf1171
yqeB
XdhC/CoxI family protein
1291280
1290942
−2
339


TM.orf1172

VWA containing CoxE family protein
1292545
1291286
−3
1260


TM.orf1173

ATPase associated with various cellular
1293426
1292542
−1
885




activities


TM.orf1174
cutM
carbon-monoxide dehydrogenase (acceptor)
1294473
1293673
−1
801


TM.orf1175
cutL
carbon monoxide dehydrogenase large chain
1296922
1294535
−3
2388


TM.orf1176
cutS
carbon monoxide dehydrogenase small chain
1297463
1296993
−2
471


TM.orf1177
ghrA
D-isomer specific 2-hydroxyacid
1298852
1297899
−2
954




dehydrogenase, NAD-binding


TM.orf1178

hypothetical protein
1299190
1298951
−3
240


TM.orf1179

hypothetical protein
1299598
1299329
−3
270


TM.orf1180
narP
two-component response regulator
1299787
1300494
1
708


TM.orf1181
appC
ABC transporter permease protein
1301407
1300499
−3
909


TM.orf1182
gsiC
binding-protein-dependent transport systems
1302342
1301404
−1
939




inner membrane component


TM.orf1183

oligopeptide/dipeptide ABC transporter,
1303335
1302349
−1
987




ATPase subunit


TM.orf1184

ABC transporter ATP binding protein
1304318
1303389
−2
930


TM.orf1185
dppA
oligopeptide ABC transpoter oligopeptide-
1305889
1304315
−3
1575




binding protein


TM.orf1186

conserved hypothetical protein
1306808
1305963
−2
846


TM.orf1187

TetR family transcriptional regulator
1306998
1307657
2
660


TM.orf1188

conserved hypothetical protein
1307758
1308891
1
1134


TM.orf1189

allophanate hydrolase
1309273
1308911
−3
363


TM.orf1190
rutB
isochorismatase family protein
1309950
1309270
−1
681


TM.orf1191
yufO
putative ABC transporter ATP-binding protein
1311546
1309969
−1
1578


TM.orf1192

isochorismatase family protein
1312235
1311543
−2
693


TM.orf1193
yufQ
ABC transporter permease protein
1313199
1312273
−1
927


TM.orf1194

putative simple sugar transport system
1314322
1313204
−3
1119




permease protein


TM.orf1195
med
simple sugar transport system substrate-
1315523
1314414
−2
1110




binding protein


TM.orf1196
ydfH
transcriptional regulator, GntR family
1315916
1316635
3
720


TM.orf1197

Urea amidolyase
1316648
1318471
3
1824


TM.orf1198

anti-sigma-factor antagonist
1318884
1318495
−1
390


TM.orf1199
mobA
molybdopterin-guanine dinucleotide
1319614
1318931
−3
684




biosynthesis protein A


TM.orf1200
sugE
small multidrug resistance protein
1319848
1320168
1
321


TM.orf1201

cold-shock DNA-binding domain-containing
1320334
1320687
1
354




protein


TM.orf1202
yiaO
TRAP dicarboxylate transporter- DctP subunit
1320940
1322001
1
1062


TM.orf1203

hypothetical protein
1322006
1322500
3
495


TM.orf1204

TRAP dicarboxylate transporter, DctM subunit
1322497
1323801
1
1305


TM.orf1205

long-chain-fatty-acid--CoA ligase
1323806
1324267
3
462


TM.orf1206
lcfB
AMP-dependent synthetase and ligase
1324240
1325400
1
1161


TM.orf1207

hypothetical protein
1325530
1325820
1
291


TM.orf1208
ygaZ
AzlC family protein
1325859
1326626
2
768


TM.orf1209

branched-chain amino acid transport
1326626
1326946
3
321


TM.orf1210
pepN
membrane alanyl aminopeptidase
1327064
1329727
3
2664


TM.orf1211

4-chloro-3-hydroxybutyrate hydrolase
1330982
1329804
−2
1179


TM.orf1212

transcriptional regulator, AraC family
1331259
1331786
2
528


TM.orf1213
thcR
transcriptional regulator, AraC family
1331747
1332259
3
513


TM.orf1214

Methyl-accepting chemotaxis protein
1333700
1332351
−2
1350


TM.orf1215
ycbC
conserved hypothetical protein
1333892
1334713
3
822


TM.orf1216
cph2
Phytochrome-like protein cph2
1334862
1337087
2
2226


TM.orf1217

YbaK/prolyl-tRNA synthetase associated
1337299
1337096
−3
204




region


TM.orf1218
ppnK
Predicted sugar kinase
1338173
1337343
−2
831


TM.orf1219
moaA
molybdenum cofactor biosynthesis protein A
1339224
1338181
−1
1044


TM.orf1220

conserved hypothetical protein
1340486
1339377
−2
1110


TM.orf1221

conserved hypothetical protein
1341012
1340800
−1
213


TM.orf1222

hypothetical protein
1341287
1341955
3
669


TM.orf1223
cheA
CheA Signal transduction histidine Kinases
1342075
1344816
1
2742




(STHK)


TM.orf1224
cheW
Chemotaxis signal transduction protein
1344813
1345310
2
498


TM.orf1225
cheY
FOG: CheY-like receiver
1345407
1345772
2
366


TM.orf1226
cheB
chemotaxis-specific methylesterase
1345820
1347007
3
1188


TM.orf1227

chemotaxis protein methyltransferase CheR
1347007
1347816
1
810


TM.orf1228
ctrA
two-component response regulator
1348673
1347942
−2
732


TM.orf1229
fliI
flagellum-specific ATP synthase
1348922
1350259
3
1338


TM.orf1230

hypothetical protein
1350266
1350706
3
441


TM.orf1231

conserved hypothetical protein
1351202
1352383
3
1182


TM.orf1232
yfiL
ABC transporter related protein
1352388
1353380
2
993


TM.orf1233

ABC transporter permease protein
1353373
1354323
1
951


TM.orf1234
ybfB
major facilitator superfamily MFS_1
1355611
1354358
−3
1254


TM.orf1235
yjdC
transcriptional regulator, TetR family protein
1356283
1355675
−3
609


TM.orf1236

conserved hypothetical protein
1356803
1356336
−2
468


TM.orf1237

conserved hypothetical protein
1357954
1356872
−3
1083


TM.orf1238
ylxH
FleN
1361632
1360847
−3
786


TM.orf1239

FlhF
1362951
1361629
−1
1323


TM.orf1240
flhA
flagellar biosynthesis pathway, component
1365160
1363037
−3
2124




FlhA


TM.orf1241
flbD
Response regulator containing CheY-like
1366774
1365245
−3
1530




receiver, AAA-type ATPase, and DNA-binding




domains


TM.orf1242
pomA
chemotaxis protein
1367656
1366889
−3
768


TM.orf1243
fliN
flagellar motor switch protein
1368111
1367758
−1
354


TM.orf1244

flagellar assembly protein H
1368888
1368181
−1
708


TM.orf1245
fliG
flagellar motor switch protein G
1369955
1368933
−2
1023


TM.orf1246
fliF
flagellar MS-ring protein
1371668
1369962
−2
1707


TM.orf1247

conserved hypothetical protein
1372410
1372048
−1
363


TM.orf1248
flgE
Flagellar hook protein flgE
1374276
1372573
−1
1704


TM.orf1249
flgD
flagellar basal-body rod modification protein
1375223
1374444
−2
780




FlgD


TM.orf1250

flagellar hook-length control protein
1378290
1375246
−1
3045


TM.orf1251
pleC
PAS/PAC sensor signal transduction histidine
1379682
1378405
−1
1278




kinase


TM.orf1252
chaC
ChaC-like protein
1380628
1379810
−3
819


TM.orf1253

acetoacetyl-CoA synthetase
1380890
1382917
3
2028


TM.orf1254
cph2
GGDEF family protein
1383065
1384003
3
939


TM.orf1255
soxR
LysR family transcriptional regulator
1384044
1384946
2
903


TM.orf1256
ccpA
cytochrome c551 peroxidase precursor
1385079
1386113
2
1035


TM.orf1257

conserved hypothetical protein
1387205
1386129
−2
1077


TM.orf1258

anthranilate synthase
1389504
1387342
−1
2163


TM.orf1259
bigR
ArsR family transcriptional regulator
1389867
1390208
2
342


TM.orf1260

YeeE/YedE family protein
1390205
1390642
3
438


TM.orf1261

membrane protein
1390709
1391170
3
462


TM.orf1262

metallo-beta-lactamase family protein
1391175
1392083
2
909


TM.orf1263

conserved hypothetical protein
1392135
1392353
2
219


TM.orf1264

sulfate transporter
1392393
1394147
2
1755


TM.orf1265

conserved hypothetical protein
1394285
1395940
3
1656


TM.orf1266

conserved hypothetical protein
1396591
1395983
−3
609


TM.orf1267

conserved hypothetical protein
1397805
1396579
−1
1227


TM.orf1268
ydeM
Anaerobic sulfatase-maturating enzyme
1399284
1397824
−1
1461




homolog ydeM


TM.orf1269

hypothetical protein
1399613
1399281
−2
333


TM.orf1270
bdlA
methyl-accepting chemotaxis protein
1401211
1399727
−3
1485


TM.orf1271

conserved hypothetical protein
1401687
1402514
2
828


TM.orf1272
dctP
TRAP dicarboxylate transporter- DctP subunit
1402599
1403675
2
1077


TM.orf1273

conserved hypothetical protein
1403734
1404342
1
609


TM.orf1274
siaT
TRAP dicarboxylate transporter, DctM subunit
1404412
1405698
1
1287


TM.orf1275

conserved hypothetical protein
1405788
1406468
2
681


TM.orf1276
gatA
Glutamyl-tRNA(Gln) amidotransferase subunit A
1406534
1407892
3
1359


TM.orf1277

amidase
1407916
1408734
1
819


TM.orf1278
namA
NADH: flavin oxidoreductase/NADH oxidase
1408859
1410016
3
1158


TM.orf1279

hypothetical protein
1410145
1410306
1
162


TM.orf1280
mntB
ABC transporter component
1410303
1411064
2
762


TM.orf1281
mntC
ABC transporter permease protein
1411083
1411967
2
885


TM.orf1282

periplasmic solute binding protein
1411964
1412929
3
966


TM.orf1283

helix-turn-helix type 11 domain-containing
1413590
1412898
−2
693




protein


TM.orf1284

conserved hypothetical protein
1413958
1413596
−3
363


TM.orf1285
Peci
enoyl-CoA hydratase/isomerase
1415250
1414009
−1
1242


TM.orf1286
lcfB
AMP-binding domain protein
1417269
1415335
−1
1935


TM.orf1287

Short-chain alcohol dehydrogenase of
1417502
1418254
3
753




unknown specificity


TM.orf1288
rutR
transcriptional regulator, TetR family protein
1418422
1419123
1
702


TM.orf1289
alsB
D-allose-binding periplasmic protein
1419446
1420483
3
1038


TM.orf1290
rbsA
ABC transporter related protein
1420601
1422166
3
1566


TM.orf1291
rbsC
inner-membrane translocator
1422163
1423200
1
1038


TM.orf1292
sorC
transcriptional regulator, DeoR family
1423261
1424268
1
1008


TM.orf1293
glpD
Glycerol-3-phosphate dehydrogenase
1424428
1426194
1
1767


TM.orf1294

aldolase protein
1426249
1427091
1
843


TM.orf1295
lyx
L-xylulose kinase protein
1427157
1428653
2
1497


TM.orf1296

putative dehydrogenase
1429452
1428658
−1
795


TM.orf1297

conserved hypothetical protein
1430636
1429497
−2
1140


TM.orf1298

TetR family transcriptional regulator
1430704
1431333
1
630


TM.orf1299

conserved hypothetical protein
1433151
1431346
−1
1806


TM.orf1300
fecI
ECF subfamily RNA polymerase sigma factor
1433359
1433928
1
570


TM.orf1301

sigma factor regulatory protein FecR/PupR
1433928
1434968
2
1041




family


TM.orf1302
irgA
TonB-dependent receptor
1435150
1437471
1
2322


TM.orf1303

hypothetical protein
1437570
1437866
2
297


TM.orf1304

conserved hypothetical protein
1437863
1439428
3
1566


TM.orf1305

hypothetical protein
1439425
1439766
1
342


TM.orf1306
ywfM
transporter ywfM
1440649
1439717
−3
933


TM.orf1307

TetR family transcriptional regulator
1441344
1440688
−1
657


TM.orf1308

hypothetical protein
1442035
1442223
1
189


TM.orf1309

conserved hypothetical protein
1442292
1442507
2
216


TM.orf1310

hypothetical protein
1442531
1442734
3
204


TM.orf1311
pqqE
Coenzyme PQQ synthesis protein E
1442877
1444391
2
1515


TM.orf1312

TonB-dependent receptor
1444961
1447168
3
2208


TM.orf1313

rhizobiocin/RTX toxin and hemolysin-type
1448826
1447252
−1
1575




calcium binding protein


TM.orf1314
cya
calcium binding hemolysin protein
1452588
1449004
−1
3585


TM.orf1315

hemolysin-type calcium-binding repeat family
1454350
1452965
−3
1386




protein


TM.orf1316
cya
proprotein convertase P
1457577
1454641
−1
2937


TM.orf1317
araQ
ABC superfamily ATP binding cassette
1458683
1457808
−2
876




transporter, membrane protein


TM.orf1318
yurN
inner membrane component of binding-
1459558
1458680
−3
879




protein-dependent transport system


TM.orf1319
ugpB
extracellular solute-binding protein
1460942
1459650
−2
1293


TM.orf1320
ugpC
ABC superfamily ATP binding cassette
1462072
1460972
−3
1101




transporter, ABC protein


TM.orf1321
glpR
transcriptional regulator, DeoR family
1462836
1462069
−1
768


TM.orf1322
ypeA
acetyltransferase, GNAT family
1463231
1463707
3
477


TM.orf1323
aprE
peptidase S8 and S53, subtilisin, kexin,
1463916
1466324
2
2409




sedolisin


TM.orf1324

WD-40 repeat protein
1467482
1466412
−2
1071


TM.orf1325

cobalamin synthesis protein, P47K
1468521
1467466
−1
1056


TM.orf1326

conserved hypothetical protein
1468785
1469552
2
768


TM.orf1327

hypothetical protein
1469561
1469698
3
138


TM.orf1328

lysine exporter protein LysE/YggA
1470401
1469760
−2
642


TM.orf1329

AsnC family transcriptional regulator
1470889
1470398
−3
492


TM.orf1330
hxuC
TonB-dependent hemoglobin
1471277
1473346
3
2070


TM.orf1331
hemP
hemin uptake protein HemP
1473394
1473591
1
198


TM.orf1332
hmuS
hemin transport protein
1473602
1474663
3
1062


TM.orf1333
hmuT
periplasmic binding protein
1474663
1475559
1
897


TM.orf1334
hmuU
putative ABC-type Fe3+-siderophore transport
1475564
1476712
3
1149




system, permease component


TM.orf1335
hmuV
ABC-type hemin transport system, ATPase
1476730
1477518
1
789




component


TM.orf1336
bioI
cytochrome P450 hydroxylase
1478788
1477532
−3
1257


TM.orf1337
mscL
large conductance mechanosensitive channel
1479044
1479484
3
441




protein


TM.orf1338

conserved hypothetical protein
1481042
1479507
−2
1536


TM.orf1339

outer membrane protein
1481600
1482310
3
711


TM.orf1340

conserved hypothetical protein
1482576
1483103
2
528


TM.orf1341
tolQ
MotA/TolQ/ExbB proton channel
1483635
1484510
2
876


TM.orf1342

hypothetical protein
1484536
1484949
1
414


TM.orf1343

hypothetical protein
1484946
1485374
2
429


TM.orf1344
tonB
TonB-like protein
1485371
1486483
3
1113


TM.orf1345

glycosyl transferase family 2
1489295
1486617
−2
2679


TM.orf1346

hypothetical protein
1489715
1489996
3
282


TM.orf1347

triacylglycerol lipase
1491806
1490001
−2
1806


TM.orf1348
glk
glucokinase
1492005
1493099
2
1095


TM.orf1349

agmatine deiminase
1493144
1494274
3
1131


TM.orf1350

pirin
1495175
1494279
−2
897


TM.orf1351
ykvY
peptidase M24
1496516
1495320
−2
1197


TM.orf1352

major facilitator transporter
1497829
1496525
−3
1305


TM.orf1353
pchR
AraC family transcriptional regulator
1498011
1499015
2
1005


TM.orf1354

cation efflux protein
1499913
1499005
−1
909


TM.orf1355
BGL2
glycoside hydrolase, family 17
1501794
1500118
−1
1677


TM.orf1356
cobC
putative threonine-phosphate decarboxylase
1502952
1501879
−1
1074


TM.orf1357
cobQ
adenosylcobyric acid synthase
1504421
1502949
−2
1473


TM.orf1358
btuB
TonB-dependent receptor
1506335
1504440
−2
1896


TM.orf1359

PRC-barrel domain-containing protein
1507420
1506827
−3
594


TM.orf1360

conserved hypothetical protein
1507743
1508477
2
735


TM.orf1361
hrpB
HrpA-like helicase
1508518
1511061
1
2544


TM.orf1362

rhodanese-related sulfurtransferase
1511485
1511084
−3
402


TM.orf1363
gcvA
LysR family transcriptional regulator
1511592
1512470
2
879


TM.orf1364

glutamyl-tRNA(Gln) amidotransferase subunit A
1512585
1513982
2
1398


TM.orf1365

insulin-cleaving metalloproteinase outer
1514113
1515390
1
1278




membrane protein


TM.orf1366

conserved hypothetical protein
1515482
1517041
3
1560


TM.orf1367

conserved hypothetical protein
1517041
1518117
1
1077


TM.orf1368

twin-arginine translocation pathway signal
1518133
1519266
1
1134


TM.orf1369
ycnE
Putative monooxygenase ycnE
1519633
1519343
−3
291


TM.orf1370

Haloacid dehalogenase domain protein
1520433
1519759
−1
675




hydrolase


TM.orf1371
mmsB
3-hydroxyisobutyrate dehydrogenase
1521512
1520613
−2
900


TM.orf1372

acyl-CoA dehydrogenase domain-containing
1522771
1521620
−3
1152




protein


TM.orf1373
mmsA
methylmalonate-semialdehyde dehydrogenase
1524365
1522869
−2
1497


TM.orf1374
mauR
transcriptional regulator
1524594
1525505
2
912


TM.orf1375

NupC family protein
1525694
1526542
3
849


TM.orf1376
yutK
Na+ dependent nucleoside transporter
1526520
1526921
2
402


TM.orf1377

radical SAM/B12 binding domain protein
1527265
1529292
1
2028


TM.orf1378

aminoglycoside phosphotransferase
1529454
1530494
2
1041


TM.orf1379
gno
short-chain dehydrogenase/reductase SDR
1530546
1531322
2
777


TM.orf1380
Mecr
nuclear receptor binding factor 1
1531420
1532424
1
1005


TM.orf1381
yhfT
fatty-acyl-CoA synthase
1532466
1534115
2
1650


TM.orf1382

TetR family transcriptional regulator
1534242
1534850
2
609


TM.orf1383
mdtA
RND family efflux transporter MFP subunit
1534942
1536090
1
1149


TM.orf1384
nolG
acriflavin resistance protein
1536146
1539379
3
3234


TM.orf1385
znuA
periplasmic solute binding protein
1540543
1539479
−3
1065


TM.orf1386
zur
ferric uptake regulator, Fur family
1540644
1541201
2
558


TM.orf1387
znuC
high-affinity zinc uptake system ATP-binding
1541198
1542064
3
867




protein ZnuC


TM.orf1388
znuB
permease of ABC zinc transporter ZnuB
1542083
1542883
3
801


TM.orf1389

conserved hypothetical protein
1543006
1544046
1
1041


TM.orf1390
potI
binding-protein-dependent transport systems
1544920
1544096
−3
825




inner membrane component


TM.orf1391
potB
binding-protein-dependent transport systems
1545792
1544917
−1
876




inner membrane component


TM.orf1392
potA
spermidine/putrescine transport system ATP-
1546891
1545794
−3
1098




binding protein


TM.orf1393

twin-arginine translocation pathway signal
1548148
1546973
−3
1176


TM.orf1394

UspA domain-containing protein
1548687
1549520
2
834


TM.orf1395

major facilitator family protein
1550818
1549541
−3
1278


TM.orf1396

phosphatase
1551026
1551763
3
738


TM.orf1397

hypothetical protein
1552567
1551773
−3
795


TM.orf1398

lipoprotein, putative
1553277
1552606
−1
672


TM.orf1399

ABC transport system substrate-binding
1554268
1553270
−3
999




protein


TM.orf1400

ABC transporter related protein
1555138
1554287
−3
852


TM.orf1401

ABC transport system permease protein
1556336
1555143
−2
1194


TM.orf1402
lcfB
malonyl-CoA synthase
1557997
1556462
−3
1536


TM.orf1403
crt
carnitinyl-CoA dehydratase
1558856
1558041
−2
816


TM.orf1404
ydfH
GntR family transcriptional regulator
1559600
1558914
−2
687


TM.orf1405

Tripartite ATP-independent periplasmic
1559821
1560336
1
516




transporter DctQ component


TM.orf1406

TRAP dicarboxylate transporter, DctM subunit
1560333
1561619
2
1287


TM.orf1407
yiiZ
TRAP dicarboxylate transporter- DctP subunit
1561698
1562741
2
1044


TM.orf1408

acyl-CoA synthase
1562725
1563960
1
1236


TM.orf1409
caiD
enoyl-CoA hydratase
1564010
1564804
3
795


TM.orf1410
lip2
lipolytic enzyme
1564915
1565889
1
975


TM.orf1411

hypothetical protein
1565897
1566424
3
528


TM.orf1412
gatA
Glutamyl-tRNA(Gln) amidotransferase subunit A
1566459
1567871
2
1413


TM.orf1413

conserved hypothetical protein
1568030
1568968
3
939


TM.orf1414
nahD
2-hydroxychromene-2-carboxylate isomerase
1569070
1569678
1
609


TM.orf1415
dkgB
oxidoreductase
1569801
1570634
2
834


TM.orf1416

nitroreductase
1571352
1570693
−1
660


TM.orf1417
pleC
two-component sensor histidine kinase
1571579
1573522
3
1944


TM.orf1418
ddpA
extracellular solute-binding protein
1573651
1575285
1
1635


TM.orf1419
dppB
ABC transporter membrane spanning protein
1575282
1576328
2
1047




(oligopeptide)


TM.orf1420
ddpC
binding-protein-dependent transport systems
1576325
1577242
3
918




inner membrane component


TM.orf1421
dppD
ABC transporter related protein
1577239
1578087
1
849


TM.orf1422

molybdopterin oxidoreductase
1581154
1578839
−3
2316


TM.orf1423

conserved hypothetical protein
1582223
1581204
−2
1020


TM.orf1424

TRAP transporter, 4TM/12TM fusion protein
1584193
1582220
−3
1974


TM.orf1425

31 kDa immunogenic protein
1585241
1584273
−2
969


TM.orf1426
yagI
Transcriptional regulator, IclR family
1586146
1585379
−3
768


TM.orf1427

conserved hypothetical protein
1586343
1587263
2
921


TM.orf1428

acetone carboxylase, gamma subunit
1587801
1587295
−1
507


TM.orf1429

acetone carboxylase alpha subunit
1590220
1587902
−3
2319


TM.orf1430
hyuA
Acetone carboxylase beta subunit; AcxA
1592448
1590271
−1
2178


TM.orf1431
stc
Fis family GAF modulated sigma54 specific
1592804
1594699
3
1896




transcriptional regulator


TM.orf1432

conserved hypothetical protein
1595182
1594706
−3
477


TM.orf1433

cation efflux protein precursor
1595344
1596237
1
894


TM.orf1434
lcfA
AMP-dependent synthetase and ligase
1596385
1598199
1
1815


TM.orf1435
grsT
thioesterase domain protein
1599028
1598234
−3
795


TM.orf1436
ppx
Exopolyphosphatase
1599825
1600829
2
1005


TM.orf1437
rlmE
23S rRNA methylase
1600879
1601694
1
816


TM.orf1438
guaB
inosine-5′-monophosphate dehydrogenase
1601944
1603401
1
1458


TM.orf1439
rsmB
tRNA and rRNA cytosine-C5-methylase
1603530
1604882
2
1353


TM.orf1440

hypothetical protein
1604974
1605618
1
645


TM.orf1441
yhiD
MgtC/SapB transporter
1605701
1606150
3
450


TM.orf1442
cpx
xanthine/uracil/vitamin C permease
1606354
1608045
1
1692


TM.orf1443
ydjP
AB hydrolase superfamily protein ydjP
1608128
1608985
3
858


TM.orf1444
yetK
transporter yetK
1609088
1610164
3
1077


TM.orf1445
guaA
bifunctional GMP synthase/glutamine
1610289
1611836
2
1548




amidotransferase protein


TM.orf1446

TetR family transcriptional regulator
1612122
1612697
2
576


TM.orf1447

3-beta hydroxysteroid
1612702
1613676
1
975




dehydrogenase/isomerase


TM.orf1448
ydjG
putative oxidoreductase
1614840
1613758
−1
1083


TM.orf1449
ycaN
LysR family transcriptional regulator
1615063
1615968
1
906


TM.orf1450
ndvA
Beta-(1-->2)glucan export ATP-
1619031
1616164
−1
2868




binding/permease protein ndvA


TM.orf1451

bacteriocin/lantibiotic ABC transporter
1621187
1619037
−2
2151


TM.orf1452

membrane-fusion protein
1622477
1621188
−2
1290


TM.orf1453

putative secreted protein
1623100
1622522
−3
579


TM.orf1454
silP
cation transport ATPase
1625423
1623330
−2
2094


TM.orf1455

conserved hypothetical protein
1626219
1627070
2
852


TM.orf1456

PEBP family protein
1627587
1627084
−1
504


TM.orf1457
yeaM
AraC family transcriptional regulator
1628424
1627657
−1
768


TM.orf1458

conserved hypothetical protein
1628546
1629337
3
792


TM.orf1459

conserved hypothetical protein
1629444
1630667
2
1224


TM.orf1460

conserved hypothetical protein
1630670
1631500
3
831


TM.orf1461

conserved hypothetical protein
1631513
1632250
3
738


TM.orf1462
yuxN
TetR family regulatory protein
1632939
1632262
−1
678


TM.orf1463

glycosyl transferase family protein
1633072
1634304
1
1233


TM.orf1464

putative integral membrane protein
1635052
1634318
−3
735


TM.orf1465

hypothetical protein
1635191
1635475
3
285


TM.orf1466
gdhA
membrane-bound PQQ-dependent
1635644
1636300
3
657




dehydrogenase, glucose/quinate/shikimate




family


TM.orf1467

sodium/hydrogen exchanger
1637571
1636267
−1
1305


TM.orf1468

chaperone protein HtpG
1637747
1638415
3
669


TM.orf1469

molybdopterin oxidoreductase, iron-sulfur
1638399
1640249
2
1851




binding subunit


TM.orf1470
hmeA
molybdopterin oxidoreductase, iron-sulfur
1640246
1641451
3
1206




binding subunit


TM.orf1471

Polysulphide reductase NrfD
1641448
1642797
1
1350


TM.orf1472

transmembrane prediction
1642790
1643350
3
561


TM.orf1473

conserved hypothetical protein
1643347
1643880
1
534


TM.orf1474

conserved hypothetical protein
1643877
1644626
2
750


TM.orf1475

conserved hypothetical protein
1644598
1644987
1
390


TM.orf1476

hypothetical protein
1644984
1645415
2
432


TM.orf1477

electron transport protein SCO1/SenC
1645412
1646215
3
804


TM.orf1478
ctaC
cytochrome c oxidase, subunit II
1646212
1647162
1
951


TM.orf1479
ctaD
cytochrome c oxidase, subunit I
1647159
1648784
2
1626


TM.orf1480
ctaE
cytochrome c oxidase subunit III
1648777
1649412
1
636


TM.orf1481

caa(3)-type oxidase, subunit IV
1649418
1649690
2
273


TM.orf1482

Sulfide: quinone oxidoreductase
1651323
1649695
−1
1629


TM.orf1483

transmembrane protein
1652416
1651634
−3
783


TM.orf1484
bigR
transcriptional regulator, ArsR family
1652914
1652513
−3
402


TM.orf1485

peroxiredoxin
1653579
1652911
−1
669


TM.orf1486
azlB
alanine catabolic operon transcriptional
1654154
1653687
−2
468




regulator protein


TM.orf1487
eamB
amino acid transporter LysE
1654270
1654920
1
651


TM.orf1488

conserved hypothetical protein
1655033
1656364
3
1332


TM.orf1489
yfkF
major facilitator superfamily MFS-1
1656361
1657545
1
1185


TM.orf1490

GATS-like protein 1
1657939
1657559
−3
381


TM.orf1491

peroxidase-related enzyme
1658554
1657970
−3
585


TM.orf1492
yhdH
zinc-binding alcohol dehydrogenase
1659600
1658608
−1
993


TM.orf1493
dhaR
TetR family transcriptional regulator
1660309
1659629
−3
681


TM.orf1494
nahR
LysR family transcriptional regulator
1661260
1660334
−3
927


TM.orf1495

conserved hypothetical protein
1661351
1661824
3
474


TM.orf1496

Alcohol dehydrogenase zinc-binding domain
1661821
1662735
1
915




protein


TM.orf1497

hypothetical protein
1662843
1663415
2
573


TM.orf1498
yozG
XRE family transcriptional regulator
1663415
1663621
3
207


TM.orf1499
yfcG
glutathione S-transferase domain-containing
1664340
1663633
−1
708




protein


TM.orf1500

conserved hypothetical protein
1665756
1664476
−1
1281


TM.orf1501
ybfL
transposase, is4 family
1665938
1667053
3
1116


TM.orf1502

ATP-dependent metalloprotease FtsH
1667172
1668986
2
1815


TM.orf1503
adhA
zinc-binding alcohol dehydrogenase family
1669071
1670054
2
984




protein


TM.orf1504

dihydroxy-acid dehydratase
1670168
1671841
3
1674


TM.orf1505
xthA
exodeoxyribonuclease III Xth
1672701
1671868
−1
834


TM.orf1506
erpA
Iron-sulfur cluster insertion protein
1673106
1672717
−1
390


TM.orf1507

dGTP triphosphohydrolase
1673308
1674513
1
1206


TM.orf1508
argS
arginyl-tRNA synthetase
1674515
1676260
3
1746


TM.orf1509

protein TonB, putative
1676260
1677294
1
1035


TM.orf1510
nagZ
Beta-N-acetylhexosaminidase
1677291
1678337
2
1047


TM.orf1511
scpA
condensin subunit ScpA
1678359
1679288
2
930


TM.orf1512
scpB
transcription regulator
1679278
1679955
1
678


TM.orf1513

membrane-fusion protein
1680119
1681375
3
1257


TM.orf1514
lagD
ATP-binding cassette subfamily C
1681372
1683636
1
2265


TM.orf1515
hlyB
ATP-binding cassette subfamily B
1683633
1686644
2
3012


TM.orf1516

conserved hypothetical protein
1687259
1686669
−2
591


TM.orf1517
bepC
type I secretion outer membrane protein, TolC
1688680
1687286
−3
1395




family


TM.orf1518

hypothetical protein
1688979
1688677
−1
303


TM.orf1519

conserved hypothetical protein
1689357
1688986
−1
372


TM.orf1520
arcA
Response regulators consisting of a CheY-like
1689771
1690445
2
675




receiver domain and a winged-helix DNA-




binding domain


TM.orf1521

hypothetical protein
1690822
1691073
1
252


TM.orf1522
PMP3
Plasma membrane proteolipid
1691208
1691393
2
186


TM.orf1523

putative proteasome-type protease
1692190
1691471
−3
720


TM.orf1524

transglutaminase domain protein
1693098
1692262
−1
837


TM.orf1525

conserved hypothetical protein
1694101
1693154
−3
948


TM.orf1526

conserved hypothetical protein
1695737
1694187
−2
1551


TM.orf1527

putative anti-sigma regulatory factor,
1696399
1695950
−3
450




serine/threonine protein kinase


TM.orf1528
btrV
anti-anti-sigma regulatory factor
1696803
1696468
−1
336


TM.orf1529
yqeW
Na+/Picotransporter
1697053
1698705
1
1653


TM.orf1530
yoaE
terC-like membrane protein
1698858
1699607
2
750


TM.orf1531

hemolysin
1699604
1700917
3
1314


TM.orf1532

hypothetical protein
1701280
1700933
−3
348


TM.orf1533

extracellular solute-binding protein, family 1
1701653
1702708
3
1056


TM.orf1534
fbpB
Fe(3+)-transport system permease protein
1702922
1704649
3
1728




sfuB


TM.orf1535

ABC transporter related protein
1704649
1705710
1
1062


TM.orf1536
tatA
Sec-independent protein translocase protein
1705796
1706044
3
249




tatA/E homolog


TM.orf1537
tatB
Sec-independent protein translocase protein
1706101
1706607
1
507




TatB


TM.orf1538
tatC
sec-independent protein translocase protein
1706614
1707447
1
834


TM.orf1539
serS
seryl-tRNA synthetase
1707547
1708836
1
1290


TM.orf1540
surE
acid phosphatase
1708986
1709741
2
756


TM.orf1541
pcm
Protein-L-isoaspartate(D-aspartate) O-
1709744
1710409
3
666




methyltransferase


TM.orf1542
ygeR
Membrane protein
1710498
1711802
2
1305


TM.orf1543
fabF
3-oxoacyl-(acyl-carrier-protein) synthase II
1713106
1711841
−3
1266




protein


TM.orf1544

transcriptional regulator, TetR family
1713711
1713118
−1
594


TM.orf1545

conserved hypothetical protein
1714692
1713799
−1
894


TM.orf1546
yajC
preprotein translocase subunit YajC
1714946
1715341
3
396


TM.orf1547
secD
Preprotein translocase subunit SecD
1715451
1717019
2
1569


TM.orf1548
secF
Preprotein translocase subunit SecF
1717039
1718007
1
969


TM.orf1549

NADH dehydrogenase [ubiquinone] 1 alpha
1718056
1718439
1
384




subcomplex assembly factor


TM.orf1550
sodB
superoxide dismutase
1719123
1718524
−1
600


TM.orf1551

conserved hypothetical protein
1720699
1719497
−3
1203


TM.orf1552
crtB
fusion protein of y4aC and y4aD
1722519
1720735
−1
1785


TM.orf1553

squalene
1722679
1723536
1
858


TM.orf1554

glucose-inhibited division protein A
1724921
1723554
−2
1368


TM.orf1555

hypothetical protein
1725496
1725029
−3
468


TM.orf1556
uvrA
excinuclease ABC subunit A
1728736
1725845
−3
2892


TM.orf1557

Methyl-accepting chemotaxis protein signaling
1730575
1728926
−3
1650




domain


TM.orf1558

conserved hypothetical protein
1731388
1730708
−3
681


TM.orf1559
yodQ
acetylornithine deacetylase
1732898
1731552
−2
1347


TM.orf1560
cmpR
LysR family transcriptional regulator
1733016
1733912
2
897


TM.orf1561
cysW
sulfate ABC transporter, permease protein
1734802
1733906
−3
897




CysW


TM.orf1562
cysU
sulfate ABC transporter, permease protein
1735666
1734827
−3
840




CysT


TM.orf1563
cysP
sulfate ABC transporter, sulfate-binding
1736684
1735674
−2
1011




protein


TM.orf1564

hypothetical protein
1736794
1736681
−3
114


TM.orf1565

beta-lactamase domain-containing protein
1738310
1736901
−2
1410


TM.orf1566
ssb
single-strand DNA binding protein
1738492
1739133
1
642


TM.orf1567

aldo/keto reductase
1740203
1739235
−2
969


TM.orf1568
ohrR
transcriptional regulator, MarR family
1740407
1740874
3
468


TM.orf1569
ohr
organic hydroperoxide resistance protein
1741000
1741413
1
414


TM.orf1570
gyrA
Type IIA topoisomerase (DNA gyrase/topo II,
1741674
1744610
2
2937




topoisomerase IV), A subunit


TM.orf1571
coaD
phosphopantetheine adenylyltransferase
1744632
1745171
2
540


TM.orf1572
queA
Queuosine biosynthesis protein
1745280
1746341
2
1062


TM.orf1573
tgt
Queuine/archaeosinetRNA-ribosyltransferase
1746338
1747522
3
1185


TM.orf1574

AMP-dependent synthetase and ligase
1749280
1747559
−3
1722


TM.orf1575
mcp2
methyl-accepting chemotaxis protein
1750175
1751881
3
1707


TM.orf1576

hypothetical protein
1752014
1752364
3
351


TM.orf1577

similar to protein conserved in bacteria with a
1752687
1753322
2
636




cystatin-like fold


TM.orf1578

Aldehyde Dehydrogenase
1754996
1753404
−2
1593


TM.orf1579

Hydroxypyruvate isomerase
1755861
1755070
−1
792


TM.orf1580

SprT protein
1755977
1756609
3
633


TM.orf1581
ldc
Orn/DAP/Arg decarboxylase 2
1757015
1758154
3
1140


TM.orf1582

haloacid dehalogenase, type II
1758963
1758262
−1
702


TM.orf1583
slt
soluble lytic murein transglycosylase precursor
1761198
1759060
−1
2139


TM.orf1584
dapA
dihydrodipicolinate synthase
1761583
1762494
1
912


TM.orf1585
smpB
SsrA-binding protein
1762550
1763026
3
477


TM.orf1586
yuiH
oxidoreductase
1763031
1763738
2
708


TM.orf1587

putative uracil-DNA glycosylase
1764448
1763729
−3
720


TM.orf1588

conserved hypothetical protein
1765409
1764588
−2
822


TM.orf1589
folK
7,8-Dihydro-6-hydroxymethylpterin-
1765659
1766156
2
498




pyrophosphokinase, HPPK


TM.orf1590
rpoZ
DNA-directed RNA polymerase, omega
1766263
1766664
1
402




subunit


TM.orf1591
rsh
Guanosine polyphosphate
1766844
1769000
2
2157




pyrophosphohydrolases/synthetases


TM.orf1592
pyrE
orotate phosphoribosyltransferase
1769087
1769689
3
603


TM.orf1593
pdxJ
Pyridoxal phosphate biosynthesis protein
1769762
1770553
3
792


TM.orf1594
acpS
4′-phosphopantetheinyl transferase
1770558
1770989
2
432


TM.orf1595
lepB
signal peptidase I
1770986
1771786
3
801


TM.orf1596
rnc
RNAse III
1771856
1772584
3
729


TM.orf1597
era
GTP-binding protein Era
1772581
1773660
1
1080


TM.orf1598

hypothetical protein
1773946
1774569
1
624


TM.orf1599

p040
1774559
1775173
3
615


TM.orf1600

hypothetical protein
1775189
1775713
3
525


TM.orf1601

conserved hypothetical protein
1776669
1775686
−1
984


TM.orf1602

transcriptional regulator protein
1776764
1777684
3
921


TM.orf1603
recO
DNA repair protein RecO
1777681
1778469
1
789


TM.orf1604
parC
Gram negative topoisomerase IV, subunit A
1778466
1780784
2
2319


TM.orf1605

TRAP dicarboxylate transporter, DctM subunit
1782490
1780913
−3
1578


TM.orf1606

tripartite ATP-independent periplasmic
1782764
1782495
−2
270




transporter DctQ


TM.orf1607

tripartite ATP-independent periplasmic
1783036
1782752
−3
285




transporter DctQ


TM.orf1608

Bacterial extracellular solute-binding protein,
1784565
1783456
−1
1110




family 7


TM.orf1609

TRAP-type transports system extracellular
1786091
1784991
−2
1101




solute binding protein


TM.orf1610

Bacterial extracellular solute-binding protein,
1787389
1786277
−3
1113




family 7


TM.orf1611
ate
Arginyltransferase
1788423
1787695
−1
729


TM.orf1612
tdcB
threonine dehydratase
1789781
1788525
−2
1257


TM.orf1613
quiP
peptidase S45 penicillin amidase
1792263
1789819
−1
2445


TM.orf1614
Odc1
lysine
1792435
1793697
1
1263


TM.orf1615
mhpD
fumarylacetoacetate (FAA) hydrolase
1794703
1793690
−3
1014


TM.orf1616
hemB
Delta-aminolevulinic acid dehydratase
1795758
1794754
−1
1005


TM.orf1617

putative 3-hydroxyisobutyryl-Coenzyme A
1796022
1797101
2
1080




hydrolase


TM.orf1618
expG
transcriptional regulator
1797343
1797798
1
456


TM.orf1619
speE
spermidine synthase
1798767
1797889
−1
879


TM.orf1620
speH
S-adenosylmethioninedecarboxylase
1799218
1798817
−3
402




proenzyme


TM.orf1621

Haloacetate dehalogenase H-1
1799576
1800466
3
891


TM.orf1622

conserved hypothetical protein
1800503
1801288
3
786


TM.orf1623

hypothetical protein
1801610
1802128
3
519


TM.orf1624
rnk
nucleoside diphosphate kinase regulator
1802160
1802576
2
417


TM.orf1625
recG
ATP-dependent DNA helicase
1804676
1802577
−2
2100


TM.orf1626
EMI5
Succinate dehydrogenase assembly factor
1804861
1805169
1
309


TM.orf1627
mfd
transcription-repair coupling factor superfamily
1805326
1808889
1
3564




II helicase


TM.orf1628

conserved hypothetical protein
1809061
1809393
1
333


TM.orf1629

hypothetical protein
1809447
1809929
2
483


TM.orf1630
pleD
response regulator/GGDEF domain protein
1811242
1809914
−3
1329


TM.orf1631
ytcI
AMP-dependent synthetase and ligase
1812810
1811293
−1
1518


TM.orf1632

methyl-accepting chemotaxis protein
1815215
1813104
−2
2112


TM.orf1633

tRNA/rRNA methyltransferase (SpoU)
1815583
1816182
1
600


TM.orf1634
pilJ
methyl-accepting chemotaxis protein
1818315
1816198
−1
2118


TM.orf1635

Transmembrane protein
1819276
1818431
−3
846


TM.orf1636

Glycosyltransferase
1820451
1819339
−1
1113


TM.orf1637

UDP-2,3-diacylglucosamine hydrolase
1821447
1820455
−1
993


TM.orf1638

cold-shock DNA-binding domain-containing
1821901
1821695
−3
207




protein


TM.orf1639

cold-shock DNA-binding domain protein
1822480
1822274
−3
207


TM.orf1640
kipR
regulatory protein, IclR
1823933
1823052
−2
882


TM.orf1641
bcpA
2,3-dimethylmalate lyase
1824793
1823930
−3
864


TM.orf1642

putative branched chain amino acid ABC
1826112
1824880
−1
1233




transporter substrate-binding protein


TM.orf1643
IivF
ABC transporter related protein
1826920
1826201
−3
720


TM.orf1644
braF
High-affinity branched-chain amino acid
1827752
1826913
−2
840




transport ATP-binding protein braF


TM.orf1645
IivM
branched chain amino acid ABC transporter
1828717
1827749
−3
969




inner membrane protein


TM.orf1646
IivH
putative ABC transporter, permease protein
1829599
1828733
−3
867


TM.orf1647
leuD
3-isopropylmalate dehydratase, small subunit
1830134
1829604
−2
531


TM.orf1648

3-isopropylmalate dehydratase large subunit
1831449
1830193
−1
1257


TM.orf1649

F0F1 ATP synthase subunit beta
1831718
1833145
3
1428


TM.orf1650

alternate F1F0 ATPase, F1 subunit epsilon
1833142
1833570
1
429


TM.orf1651

F0F1-ATPase subunit
1833587
1833877
3
291


TM.orf1652

hypothetical protein
1833874
1834194
1
321


TM.orf1653

F0F1 ATP synthase subunit A
1834191
1834925
2
735


TM.orf1654

F0F1 ATP synthase subunit C
1834922
1835170
3
249


TM.orf1655

H + transporting two-sector ATPase B/B′
1835184
1835927
2
744




subunit


TM.orf1656

F0F1 ATP synthase subunit alpha
1835914
1837476
1
1563


TM.orf1657
atpG
H + transporting two-sector ATPase gamma
1837473
1838354
2
882




subunit


TM.orf1658

hypothetical protein
1838654
1838367
−2
288


TM.orf1659

major facilitator superfamily MFS_1
1840067
1838769
−2
1299


TM.orf1660

TfoX domain-containing protein
1840283
1840675
3
393


TM.orf1661

conserved hypothetical protein
1840779
1842725
2
1947


TM.orf1662
ppdK
Pyruvate, phosphate dikinase
1842737
1844335
3
1599


TM.orf1663
yehW
binding-protein-dependent transport systems
1845088
1844321
−3
768




inner membrane component


TM.orf1664
yehX
ABC transporter related protein
1846020
1845085
−1
936


TM.orf1665
yehY
binding-protein-dependent transport systems
1847249
1846017
−2
1233




inner membrane component


TM.orf1666
osmF
Substrate-binding region of ABC-type glycine
1848202
1847246
−3
957




betaine transport system


TM.orf1667

putative membrane protein
1848844
1848488
−3
357


TM.orf1668

response regulator
1849103
1850494
3
1392


TM.orf1669

lipolytic enzyme
1851479
1850499
−2
981


TM.orf1670
vapI
XRE family plasmid maintenance system
1851988
1852281
1
294




antidote protein


TM.orf1671

conserved hypothetical protein
1852615
1852304
−3
312


TM.orf1672

transposase, mutator type
1852717
1853367
1
651


TM.orf1673

transposase, mutator type
1853497
1853646
1
150


TM.orf1674
mltB
Lytic murein transglycosylase
1855361
1856512
3
1152


TM.orf1675
rlpA
Lipoproteins
1856642
1857619
3
978


TM.orf1676
dacA
serine-type D-Ala-D-Ala carboxypeptidase
1857693
1858931
2
1239


TM.orf1677
tmk
thymidylate kinase
1858943
1859620
3
678


TM.orf1678
holB
ATPase involved in DNA replication
1859617
1860744
1
1128


TM.orf1679
metG
methionyl-tRNA synthetase
1860830
1862380
3
1551


TM.orf1680
ycfH
TatD-related deoxyribonuclease
1862436
1863242
2
807


TM.orf1681
lipB
metallo-beta-lactamase superfamily
1863242
1864087
3
846


TM.orf1682

hypothetical protein
1864096
1864536
1
441


TM.orf1683

regulatory protein TetR
1865132
1864539
−2
594


TM.orf1684
ydgK
drug resistance transporter, Bcr/CflA subfamily
1866381
1865194
−1
1188


TM.orf1685

conserved hypothetical protein
1866575
1866916
3
342


TM.orf1686
rpoE
RNA polymerase, sigma-24 subunit, ECF
1866987
1867547
2
561




subfamily


TM.orf1687

conserved hypothetical protein
1867531
1868301
1
771


TM.orf1688

conserved hypothetical protein
1868393
1869109
3
717


TM.orf1689

conserved hypothetical protein
1869258
1869860
2
603


TM.orf1690

acetyltransferase
1870904
1870125
−2
780


TM.orf1691
dnaB
replicative DNA helicase
1871863
1871147
−3
717


TM.orf1692
yvbT
conserved hypothetical protein
1872145
1873167
1
1023


TM.orf1693
mazG
MazG family protein
1873366
1874289
1
924


TM.orf1694
ydfG
short-chain dehydrogenase/reductase SDR
1874289
1874606
2
318


TM.orf1695
ydfG
short-chain dehydrogenase/reductase SDR
1874603
1875043
3
441


TM.orf1696
groL
unnamed protein product
1876885
1875239
−3
1647


TM.orf1697
groS
chaperonin
1877294
1877004
−2
291


TM.orf1698
hflX
GTP-binding protein HFLX
1878805
1877438
−3
1368


TM.orf1699
hfq
RNA-binding protein Hfq
1879268
1878990
−2
279


TM.orf1700

HAD-superfamily hydrolase, subfamily IA,
1880150
1879434
−2
717




variant 1


TM.orf1701
trkA
Trk system potassium uptake protein
1881552
1880176
−1
1377


TM.orf1702
ntrX
response regulator containing CheY-like
1882971
1881586
−1
1386




receiver


TM.orf1703

Nitrogen regulation protein, NtrY, Signal
1885423
1882976
−3
2448




transduction histidine kinase


TM.orf1704
ntrC
two-component response regulator, nitrogen
1886904
1885420
−1
1485




regulation response


TM.orf1705

nitrogen regulation protein
1888063
1886921
−3
1143


TM.orf1706
dus
tRNA-dihydrouridine synthase
1889211
1888213
−1
999


TM.orf1707

IspD/ispF bifunctional enzyme
1889399
1890658
3
1260


TM.orf1708
pgpA
phosphatidylglycerophosphatase A and
1890655
1891176
1
522




related protein


TM.orf1709
ygaD
protein (competence- and mitomycin-induced)
1891199
1891690
3
492


TM.orf1710

sigma factor, sigma 70 type, group 4 (ECF)
1892690
1891716
−2
975


TM.orf1711

conserved hypothetical protein
1893151
1892687
−3
465


TM.orf1712

oligoketide cyclase/lipid transport protein
1893770
1893270
−2
501


TM.orf1713
lipA
lipoic acid synthetase
1894835
1893879
−2
957


TM.orf1714
lpd
dihydrolipoamide dehydrogenase
1896793
1894970
−3
1824


TM.orf1715

pyruvate dehydrogenase complex
1898249
1896852
−2
1398




dihydrolipoamide acetyltransferase


TM.orf1716
pdhB
Pyruvate dehydrogenase E1 component, beta
1899277
1898291
−3
987




subunit


TM.orf1717
pdhA
2-dehydro-3-deoxyphosphooctonate aldolase
1900396
1899374
−3
1023


TM.orf1718
yybE
transcriptional regulator, LysR family
1901444
1900551
−2
894


TM.orf1719

putative enoyl-CoA hydratase echA8
1901579
1902382
3
804


TM.orf1720

Peptidoglycan-binding domain 1 protein
1903739
1902756
−2
984


TM.orf1721

septum formation initiator
1904226
1903840
−1
387


TM.orf1722
amiC
ABC-type branched-chain amino acid
1904609
1905880
3
1272




transport system, periplasmic component


TM.orf1723
Iiv
High-affinity branched-chain amino acid
1906153
1907892
1
1740




transport system permease protein IivH


TM.orf1724
IivM
branched chain amino acid ABC transporter
1907897
1909099
3
1203




permease


TM.orf1725
braF
putative ATP-binding component of ABC
1909096
1909899
1
804




transporter


TM.orf1726
IivF
ABC transporter, nucleotide binding/ATPase
1909915
1910610
1
696




protein (urea/amide)


TM.orf1727
ureD
urease accessory protein UreD
1910629
1911585
1
957


TM.orf1728
ureA
urease, gamma subunit
1911653
1911955
3
303


TM.orf1729
ureB
urease subunit beta
1911966
1912364
2
399


TM.orf1730
ureC
Urease alpha subunit
1912369
1914078
1
1710


TM.orf1731
ureE
urease accessory protein
1914190
1915035
1
846


TM.orf1732
ureF
Urease accessory protein UreF
1915043
1915798
3
756


TM.orf1733
ureG
urease accessory protein
1915852
1916511
1
660


TM.orf1734

conserved hypothetical protein
1917954
1916518
−1
1437


TM.orf1735

glycosyl transferase WecB/TagA/CpsF
1918745
1917951
−2
795


TM.orf1736

polysaccharide export protein
1919529
1918765
−1
765


TM.orf1737
cpsD
lipopolysaccharide biosynthesis
1921541
1919529
−2
2013


TM.orf1738

conserved hypothetical protein
1922273
1923637
3
1365


TM.orf1739
wcaJ
sugar transferase
1923693
1925129
2
1437


TM.orf1740

glycoside hydrolase family 18
1925129
1926148
3
1020


TM.orf1741

FkbM family methyltransferase
1927010
1926171
−2
840


TM.orf1742

hypothetical protein
1927033
1927158
1
126


TM.orf1743

conserved hypothetical protein
1927269
1927676
2
408


TM.orf1744
dgdR
LysR family transcriptional regulator
1928536
1927688
−3
849


TM.orf1745

Membrane protein
1928691
1929641
2
951


TM.orf1746
eno
enolase
1930955
1929666
−2
1290


TM.orf1747
kdsA
2-dehydro-3-deoxyphosphooctonate aldolase
1931957
1931103
−2
855


TM.orf1748
pyrG
CTP synthase
1933636
1932002
−3
1635


TM.orf1749

hypothetical protein
1934265
1933840
−1
426


TM.orf1750
tpiA
triose-phosphate isomerase
1935114
1934356
−1
759


TM.orf1751
ppiD
peptidyl-prolyl cis-trans isomerse
1935472
1937385
1
1914


TM.orf1752
trpE
Anthranilate synthase component I
1937456
1938973
3
1518


TM.orf1753
guaA
putative GMP synthase [glutamine-
1938970
1939551
1
582




hydrolyzing]


TM.orf1754
copZ
heavy metal transport/detoxification protein
1939783
1939574
−3
210


TM.orf1755
yibQ
conserved hypothetical protein
1941474
1939789
−1
1686


TM.orf1756
hmrR
Cu(I)-responsive transcriptional regulator
1941993
1941529
−1
465


TM.orf1757
copA
copper-translocating P-type ATPase
1944254
1941990
−2
2265


TM.orf1758
trpG
anthranilate synthase component II
1944525
1945148
2
624


TM.orf1759
trpD
anthranilate phosphoribosyltransferase
1945145
1946185
3
1041


TM.orf1760
trpC
indole-3-glycerol phosphate synthase
1946190
1947005
2
816


TM.orf1761
moaC
molybdenum cofactor biosynthesis protein
1947011
1947508
3
498




MoaC


TM.orf1762
moeA
molybdopterin biosynthesis protein
1947547
1948782
1
1236


TM.orf1763
lexA
LexA repressor
1949031
1949879
2
849


TM.orf1764

ComEC/Rec2-related protein
1952111
1949964
−2
2148


TM.orf1765

Glutamyl-tRNA synthetase, class Ic
1952232
1953629
2
1398


TM.orf1766
plpC
NLPA lipoprotein
1954013
1954648
3
636


TM.orf1767

conserved hypothetical protein
1954721
1956109
3
1389


TM.orf1768
lpxB
lipid-A-disaccharide synthase
1957339
1956149
−3
1191


TM.orf1769

conserved hypothetical protein
1958160
1957336
−1
825


TM.orf1770
lpxA
UDP-N-acetylglucosamine acyltransferase
1958996
1958157
−2
840


TM.orf1771
fabZ
beta-hydroxyacyl-(acyl-carrier-protein)
1959469
1958993
−3
477




dehydratase FabZ


TM.orf1772
lpxD
UDP-3-O-[3-hydroxymyristoyl] glucosamine N-
1960565
1959534
−2
1032




acyltransferase


TM.orf1773

outer membrane protein
1961264
1960677
−2
588


TM.orf1774
yaeT
outer membrane protein
1963560
1961269
−1
2292


TM.orf1775

zinc metalloprotease Atu1380
1964840
1963683
−2
1158


TM.orf1776
dxr
1-deoxy-D-xylulose 5-phosphate
1965945
1964890
−1
1056




reductoisomerase


TM.orf1777
cdsA
CDP-diglyceride synthetase
1966871
1966098
−2
774


TM.orf1778
uppS
undecaprenyl pyrophosphate synthetase
1967679
1966966
−1
714


TM.orf1779
frr
ribosome recycling factor
1968277
1967717
−3
561


TM.orf1780
pyrH
uridylate kinase
1969009
1968281
−3
729


TM.orf1781
tsf
elongation factor Ts
1970195
1969272
−2
924


TM.orf1782
rpsB
30S ribosomal protein S2
1971239
1970433
−2
807


TM.orf1783

DNA polymerase III alpha subunit
1975030
1971530
−3
3501


TM.orf1784

conserved hypothetical protein
1975621
1975127
−3
495


TM.orf1785

conserved hypothetical protein
1976125
1975841
−3
285


TM.orf1786
ptxR
LysR family transcriptional regulator
1977125
1976223
−2
903


TM.orf1787
nylA
6-aminohexanoate-cyclic-dimer hydrolase
1978624
1977197
−3
1428


TM.orf1788
nreC
transcriptional regulator, LuxR family
1979695
1978703
−3
993


TM.orf1789
rpsI
30S ribosomal protein S9
1980333
1979839
−1
495


TM.orf1790
rplM
Ribosomal protein L13
1980799
1980341
−3
459


TM.orf1791

Enoyl-CoA hydratase/carnithine racemase
1981104
1981913
2
810


TM.orf1792
yccU
O-acetylhomoserine/O-acetylserine
1981918
1982421
1
504




sulfhydrylase


TM.orf1793
cysD
O-acetylhomoserine sulfhydrylase
1982418
1983734
2
1317


TM.orf1794
phoE
phosphoglycerate mutase family protein
1983773
1984378
3
606


TM.orf1795
azoR
FMN-dependent NADH-azoreductase
1985030
1984401
−2
630


TM.orf1796

transcriptional regulator, LysR family
1985165
1986124
3
960


TM.orf1797

flavin reductase-like, FMN-binding
1986115
1986681
1
567


TM.orf1798
dgdR
transcriptional regulator, LysR family protein
1987558
1986707
−3
852


TM.orf1799
dgdA
aminotransferase class-Ill
1987691
1988995
3
1305


TM.orf1800

conserved hypothetical protein
1989035
1989901
3
867


TM.orf1801

glutamine amidotransferase, class
1989950
1991113
3
1164




II/dipeptidase


TM.orf1802

conserved hypothetical protein
1991182
1992288
1
1107


TM.orf1803

TRAP-T family transporter, DctQ (4 TMs)
1992301
1992771
1
471




subunit


TM.orf1804

TRAP-T family protein transporter, DctM (12
1992768
1994075
2
1308




TMs) subunit


TM.orf1805

conserved hypothetical protein
1995126
1994083
−1
1044


TM.orf1806

HD superfamily metal-dependent
1996391
1995123
−2
1269




phosphohydrolase


TM.orf1807

divalent cation tolerance protein
1997241
1996522
−1
720


TM.orf1808

transcriptional regulator, MerR family
1999060
1998188
−3
873


TM.orf1809
ihfA
integration host factor subunit alpha
1999445
1999101
−2
345


TM.orf1810
fabH
3-oxoacyl-[acyl-carrier-protein] synthase III
2000583
1999606
−1
978


TM.orf1811
plsX
putative glycerol-3-phosphate acyltransferase
2001644
2000580
−2
1065




PlsX


TM.orf1812
rpmF
Ribosomal protein L32
2001904
2001719
−3
186


TM.orf1813

conserved hypothetical protein
2002635
2002018
−1
618


TM.orf1814
uqcc
ubiquinol-cytochrome C chaperone
2003360
2002755
−2
606


TM.orf1815

conserved hypothetical protein
2003577
2004143
2
567


TM.orf1816

hypothetical protein
2004690
2004232
−1
459


TM.orf1817

conserved hypothetical protein
2005305
2004736
−1
570


TM.orf1818
thiL
thiamine-monophosphate kinase
2006528
2005488
−2
1041


TM.orf1819
nusB
transcription antitermination protein NusB
2007118
2006525
−3
594


TM.orf1820

6,7-dimethyl-8-ribityllumazine synthase
2007584
2007135
−2
450


TM.orf1821

GTP cyclohydrolase II
2008743
2007592
−1
1152


TM.orf1822
ribE
riboflavin synthase, alpha subunit
2009425
2008793
−3
633


TM.orf1823
ribD
riboflavin biosynthesis protein ribD
2010538
2009504
−3
1035


TM.orf1824

Transcriptional repressor nrdR
2011124
2010648
−2
477


TM.orf1825
glyA
serine hydroxymethyltransferase
2012503
2011205
−3
1299


TM.orf1826
ywlF
ribose 5-phosphate isomerase RpiB
2013101
2012658
−2
444


TM.orf1827

transcriptional regulatory protein MucR
2013596
2014069
3
474


TM.orf1828

hypothetical protein
2014465
2014229
−3
237


TM.orf1829

hypothetical protein
2015327
2014479
−2
849


TM.orf1830

hypothetical protein
2016262
2015324
−3
939


TM.orf1831

hypothetical protein
2016565
2016266
−3
300


TM.orf1832

conserved hypothetical protein
2016880
2016608
−3
273


TM.orf1833
aspS
Aspartyl-tRNA synthetase
2018935
2017109
−3
1827


TM.orf1834
rnd
Ribonuclease D
2019195
2020463
2
1269


TM.orf1835

putative glyoxalase/bleomycin resistance
2021259
2020492
−1
768




protein/dioxygenase family protein


TM.orf1836

chromosomal replication initiator
2021912
2021256
−2
657


TM.orf1837

permease
2023120
2021981
−3
1140


TM.orf1838

CDP-alcohol phosphatidyltransferase
2023757
2023140
−2
618


TM.orf1839

conserved hypothetical protein
2025005
2023761
−2
1245


TM.orf1840
purM
Phosphoribosylformylglycinamidine cyclo-
2025286
2026419
1
1134




ligase


TM.orf1841
GART
phosphoribosylglycinamide formyltransferase
2026392
2027057
2
666


TM.orf1842
ndk
Nucleoside diphosphate kinase
2027572
2027150
−3
423


TM.orf1843
yheS
ABC transporter, ATP-binding protein
2027829
2029742
2
1914


TM.orf1844
walK
PAS/PAC sensor hybrid histidine kinase
2030545
2032674
1
2130


TM.orf1845

DNA polymerase III subunit chi
2033165
2032686
−2
480


TM.orf1846
pepA
leucyl aminopeptidase
2034667
2033183
−3
1485


TM.orf1847

Predicted permeases
2034999
2036126
2
1128


TM.orf1848

putative permease
2036123
2037277
3
1155


TM.orf1849
lptD
Organic solvent tolerance protein OstA
2037280
2039472
1
2193


TM.orf1850
surA
Parvulin-like peptidyl-prolyl isomerase
2039504
2040784
3
1281


TM.orf1851
pdxA
dimethyladenosine transferase
2040771
2041805
2
1035


TM.orf1852
rsmA
dimethyladenosine transferase
2041853
2042728
3
876


TM.orf1853
yrbG
sodium/calcium exchanger
2042829
2043818
2
990


TM.orf1854
gmk
Guanylate kinase (GMP kinase)
2044522
2043848
−3
675


TM.orf1855
yicC
stress-induced protein
2045441
2044551
−2
891


TM.orf1856

Short-chain dehydrogenase/reductase (SDR)
2045603
2046376
3
774




superfamily


TM.orf1857
btuB
TonB-dependent receptor
2046799
2048769
1
1971


TM.orf1858

periplasmic binding protein
2048773
2049651
1
879


TM.orf1859
yvrB
transport system permease protein
2049648
2050643
2
996


TM.orf1860
fecE
iron(III) dicitrate transport ATP-binding protein
2050647
2051492
2
846




FecE


TM.orf1861

conserved hypothetical protein
2051447
2052490
3
1044


TM.orf1862

periplasmic solute-binding protein
2053517
2052495
−2
1023


TM.orf1863
fabF
3-oxoacyl-[acyl-carrier-protein] synthase II
2054827
2053568
−3
1260


TM.orf1864
acpP
transit peptide-acyl carrier protein fusion
2055348
2055112
−1
237




protein


TM.orf1865
nodG
dehydrogenase
2056285
2055548
−3
738


TM.orf1866
fabD
S-malonyltransferase
2057284
2056343
−3
942


TM.orf1867

hypothetical protein
2057425
2057538
1
114


TM.orf1868
rpsF
30S ribosomal protein S6
2057880
2058332
2
453


TM.orf1869
rpsR
small subunit ribosomal protein S18
2058365
2058640
3
276


TM.orf1870

conserved hypothetical protein
2058801
2059754
2
954


TM.orf1871
rplI
50S ribosomal protein L9
2059777
2060346
1
570


TM.orf1872
cfa
cyclopropane-fatty-acyl-phospholipid synthase
2060777
2062018
3
1242


TM.orf1873
dnaB
replicative DNA helicase
2062180
2063763
1
1584


TM.orf1874
alr
alanine racemase
2063760
2064986
2
1227


TM.orf1875

putative ABC transporter permease protein
2064983
2065756
3
774


TM.orf1876

putative ribonucleotide transport ATP-binding
2065791
2066570
2
780




protein


TM.orf1877
radA
DNA repair protein RadA
2066607
2068079
2
1473


TM.orf1878
cvpA
CvpA family protein
2068136
2068903
3
768


TM.orf1879
purF
amidophosphoribosyltransferase
2068966
2070456
1
1491


TM.orf1880
yciK
short-chain dehydrogenase/reductase SDR
2070503
2071327
3
825


TM.orf1881

regulatory protein TetR
2072065
2071397
−3
669


TM.orf1882

hypothetical protein
2072479
2072607
1
129


TM.orf1883
SRY1
amino-acid dehydratase protein
2073857
2072868
−2
990


TM.orf1884
engA
GTP-binding protein EngA
2075421
2073862
−1
1560


TM.orf1885
yfgL
PQQ enzyme repeat family protein
2076972
2075596
−1
1377


TM.orf1886

conserved hypothetical protein
2077694
2076969
−2
726


TM.orf1887

2′-5′ RNA ligase
2079212
2079949
3
738


TM.orf1888

alpha/beta fold family hydrolase
2079217
2077826
−3
1392


TM.orf1889

conserved hypothetical protein
2080076
2081422
3
1347


TM.orf1890
qor
oxidoreductase, zinc-binding dehydrogenase
2082477
2081425
−1
1053




family


TM.orf1891
virF
transcriptional regulator, AraC family
2083450
2082566
−3
885


TM.orf1892
pbuA
TonB-dependent siderophore receptor
2083700
2085835
3
2136


TM.orf1893

conserved hypothetical protein
2085867
2086160
2
294


TM.orf1894
alkB
alkylated DNA repair protein
2086811
2086161
−2
651


TM.orf1895

conserved hypothetical protein
2087542
2086829
−3
714


TM.orf1896
ttuE
pyruvate kinase
2089060
2087633
−3
1428


TM.orf1897

putative helicase/glycosylase
2090031
2089057
−1
975


TM.orf1898

conserved hypothetical protein
2091276
2090047
−1
1230


TM.orf1899

Zinc transporter
2092271
2091309
−2
963


TM.orf1900
sldA
glucose dehydrogenase
2094700
2092283
−3
2418


TM.orf1901

quinolinate synthetase complex, A subunit
2094913
2095887
1
975


TM.orf1902
nadB
L-aspartate oxidase
2095893
2097410
2
1518


TM.orf1903
nadC
nicotinate-nucleotide pyrophosphorylase
2097397
2098254
1
858


TM.orf1904

multi-sensor signal transduction histidine
2098362
2099126
2
765




kinase


TM.orf1905
rpfG
putative PAS/PAC sensor protein
2099123
2099464
3
342


TM.orf1906

SMP-30/Gluconolaconase/LRE domain-
2099618
2100664
3
1047




containing protein


TM.orf1907

conserved hypothetical protein
2101439
2100675
−2
765


TM.orf1908
valS
Valyl-tRNA synthetase
2104205
2101536
−2
2670


TM.orf1909

conserved hypothetical protein
2105076
2104375
−1
702


TM.orf1910
bepC
TolC family type I secretion outer membrane
2106603
2105257
−1
1347




protein


TM.orf1911
pcm2
protein-L-isoaspartate(D-aspartate) O-
2107357
2106701
−3
657




methyltransferase


TM.orf1912

hypothetical protein
2107815
2107594
−1
222


TM.orf1913
alkK
medium-chain-fatty-acid--CoA ligase
2108418
2110052
2
1635


TM.orf1914
SCP2
sterol carrier family protein
2110578
2110285
−1
294


TM.orf1915
dhkK
multi-sensor hybrid histidine kinase
2110886
2113477
3
2592


TM.orf1916

conserved hypothetical protein
2114158
2113478
−3
681


TM.orf1917
pulE
general secretion pathway protein E
2114300
2115988
3
1689


TM.orf1918

hypothetical protein
2115985
2117280
1
1296


TM.orf1919

hypothetical protein
2117277
2118068
2
792


TM.orf1920

hypothetical protein
2118220
2118795
1
576


TM.orf1921

hypothetical protein
2118990
2120030
2
1041


TM.orf1922
outD
type II and III secretion system protein
2120027
2121799
3
1773


TM.orf1923

conserved hypothetical protein
2121796
2122551
1
756


TM.orf1924
pleC
Sensor protein
2126181
2122729
−1
3453


TM.orf1925

elongation factor G
2128635
2126509
−1
2127


TM.orf1926

hypothetical protein
2129444
2130598
3
1155


TM.orf1927
imuB
nucleotidyltransferase/DNA polymerase
2130673
2132406
1
1734




involved in DNA repair


TM.orf1928

DNA polymerase III subunit alpha
2132460
2135711
2
3252


TM.orf1929

phospholipid N-methyltransferase
2135982
2136569
2
588


TM.orf1930
pdhS
Sensor protein
2138390
2136588
−2
1803


TM.orf1931

SpoVR family protein
2140127
2138673
−2
1455


TM.orf1932

conserved hypothetical protein
2141480
2140197
−2
1284


TM.orf1933
prkA
PrkA serine kinase
2143509
2141578
−1
1932


TM.orf1934

hypothetical protein
2144138
2143938
−2
201


TM.orf1935

hypothetical protein
2144325
2144131
−1
195


TM.orf1936

GNAT family acetyltransferase
2144648
2144277
−2
372


TM.orf1937

conserved hypothetical protein
2145649
2144921
−3
729


TM.orf1938

periplasmic binding protein
2146284
2147204
2
921


TM.orf1939
yfiZ
transport system permease protein
2147201
2148211
3
1011


TM.orf1940
yclP
ABC transporter related protein
2148205
2148993
1
789


TM.orf1941
recJ
single-stranded-DNA-specificexonuclease
2150814
2149024
−1
1791


TM.orf1942
ywjI
fructose 1,6-bisphosphatase II
2151844
2150867
−3
978


TM.orf1943
hom
homoserine dehydrogenase
2153434
2152106
−3
1329


TM.orf1944
aatC
aminotransferase
2154657
2153431
−1
1227


TM.orf1945
phbC
poly-beta-hydroxybutyrate polymerase
2155556
2157358
3
1803


TM.orf1946
yeiE
LysR family transcriptional regulator
2158296
2157418
−1
879


TM.orf1947
ydgK
drug resistance transporter, Bcr/CflA subfamily
2159519
2158293
−2
1227


TM.orf1948

membrane protein
2160635
2159586
−2
1050


TM.orf1949

hexapaptide repeat-containing transferase
2161402
2160773
−3
630


TM.orf1950
lolD
ABC transporter related protein
2162133
2161450
−1
684


TM.orf1951
lolC
lipoprotein-releasing system permease protein
2163373
2162126
−3
1248


TM.orf1952
pcm
Protein-L-isoaspartate(D-aspartate) O-
2163558
2164793
2
1236




methyltransferase (PCMT) family


TM.orf1953

conserved hypothetical protein
2165229
2164807
−1
423


TM.orf1954

xanthine/uracil/vitamin C permease
2165468
2166760
3
1293


TM.orf1955
yeaM
AraC family transcriptional regulator
2168105
2167317
−2
789


TM.orf1956
qacA
MFS family transporter
2168260
2169447
1
1188


TM.orf1957
CRYZ
Alcohol dehydrogenase zinc-binding domain
2170488
2169475
−1
1014




protein


TM.orf1958
pecT
lysR-like transcriptional regulator
2170607
2171488
3
882


TM.orf1959
rhtC
lysine exporter family protein
2171560
2172219
1
660


TM.orf1960

conserved hypothetical protein
2172465
2172226
−1
240


TM.orf1961

methylmalonyl-CoA epimerase
2172974
2172570
−2
405


TM.orf1962
rnjA
beta-lactamase domain-containing protein
2174803
2173139
−3
1665


TM.orf1963
coaX
pantothenate kinase
2175615
2174836
−1
780


TM.orf1964

biotin-
2176456
2175644
−3
813


TM.orf1965
nuoN
NADH dehydrogenase subunit N
2177898
2176453
−1
1446


TM.orf1966
nuoM
NADH dehydrogenase subunit M
2179443
2177920
−1
1524


TM.orf1967

NADH dehydrogenase subunit L
2181374
2179443
−2
1932


TM.orf1968
nuoK
NADH dehydrogenase subunit K
2181690
2181382
−1
309


TM.orf1969
nuoJ
NADH-quinone oxidoreductase chain 10
2182304
2181690
−2
615


TM.orf1970
nuoI
NADH dehydrogenase subunit I
2182900
2182412
−3
489


TM.orf1971
nuoH
NADH dehydrogenase subunit G
2184067
2183054
−3
1014


TM.orf1972

NADH-quinone oxidoreductase, chain G
2186138
2184060
−2
2079


TM.orf1973

NADH dehydrogenase I subunit F
2187429
2186149
−1
1281


TM.orf1974

NADH dehydrogenase I chain E
2188045
2187419
−3
627


TM.orf1975
nuoD
NADH dehydrogenase I chain D
2189247
2188060
−1
1188


TM.orf1976
nuoC
NADH (or F420H2) dehydrogenase, subunit C
2189798
2189244
−2
555


TM.orf1977
nuoB
NADH-quinone oxidoreductase, B subunit
2190524
2189967
−2
558


TM.orf1978

NADH-ubiquinone/plastoquinone
2190886
2190521
−3
366




oxidoreductase, chain 3


TM.orf1979

putative flavin-binding monooxygenase
2193069
2191516
−1
1554


TM.orf1980
hup
histone-like DNA-binding protein
2193901
2193626
−3
276


TM.orf1981
lon
ATP-dependent protease La
2196599
2194116
−2
2484


TM.orf1982
clpX
ATP-dependent protease ATP-binding subunit
2198022
2196754
−1
1269


TM.orf1983
clpP
Protease subunit of ATP-dependent Clp
2199088
2198456
−3
633




proteases


TM.orf1984
tig
FKBP-type peptidyl-prolyl cis-trans isomerase
2200572
2199214
−1
1359




(trigger factor)


TM.orf1985

conserved hypothetical protein
2202443
2200824
−2
1620


TM.orf1986
exoZ
exopolysaccharide production
2203626
2202583
−1
1044




(acetyltransferase) protein


TM.orf1987

malonyl-CoA decarboxylase
2205347
2203839
−2
1509


TM.orf1988

conserved hypothetical protein
2207017
2205452
−3
1566


TM.orf1989

hypothetical protein
2207192
2207560
3
369


TM.orf1990

endonuclease/exonuclease/phosphatase
2207572
2208174
1
603


TM.orf1991
ybdL
possible cysteine-S-conjugate beta-lyase
2209649
2208462
−2
1188


TM.orf1992
glnB
Nitrogen regulatory protein P-II
2210083
2210421
1
339


TM.orf1993
glnA
L-glutamine synthetase
2210677
2212086
1
1410


TM.orf1994

conserved hypothetical protein
2213194
2212262
−3
933


TM.orf1995
lysR
transcriptional regulator, LysR family
2213332
2214294
1
963


TM.orf1996
parE
topoisomerase IV subunit B
2214825
2216804
2
1980


TM.orf1997

hypothetical protein
2216898
2217503
2
606


TM.orf1998

hypothetical protein
2217551
2218081
3
531


TM.orf1999

hypothetical protein
2218105
2218752
1
648


TM.orf2000

hypothetical protein
2218981
2218829
−3
153


TM.orf2001

chemotaxis sensory transducer
2219272
2220630
1
1359


TM.orf2002

hypothetical protein
2220928
2220659
−3
270


TM.orf2003
phnM
putative metal-dependent hydrolase involved
2220969
2221925
2
957




in phosphonate metabolism; PhnM protein


TM.orf2004
bioH
hydrolase, alpha
2222639
2221926
−2
714


TM.orf2005

isochorismatase domain-containing protein 2A
2223264
2222671
−1
594


TM.orf2006
rhlE
DeaD
2223380
2224945
3
1566


TM.orf2007

hydrolase or acyltransferase
2226033
2225167
−1
867


TM.orf2008

putative amino acid or sugar ABC transport
2227092
2226037
−1
1056




system, permease protein


TM.orf2009
braD
putative amino acid or sugar ABC transport
2228115
2227108
−1
1008




system, permease protein


TM.orf2010
IivF
High-affinity branched-chain amino acid
2228837
2228112
−2
726




transport ATP-binding protein


TM.orf2011
braF
ABC transporter ATPase
2229627
2228830
−1
798


TM.orf2012

branched-chain amino acid ABC transporter,
2231062
2229866
−3
1197




periplasmic branched-chain amino acid-




binding protein


TM.orf2013
hyfR
PAS modulated sigma54 specific
2232828
2231296
−1
1533




transcriptional regulator, Fis family


TM.orf2014
besA
putative esterase
2233914
2232955
−1
960


TM.orf2015
mdlC
thiamine pyrophosphate binding domain-
2235657
2233963
−1
1695




containing protein


TM.orf2016
hmp
oxidoreductase FAD/NAD(P)-binding subunit
2236949
2235744
−2
1206


TM.orf2017

conserved hypothetical protein
2237212
2238534
1
1323


TM.orf2018
wcaL
glucosyltransferase
2238557
2239822
3
1266


TM.orf2019

Glycosyl transferase, group 1
2239822
2241012
1
1191


TM.orf2020

phosphoglycerate mutase
2241009
2241626
2
618


TM.orf2021

conserved hypothetical protein
2241641
2242849
3
1209


TM.orf2022

conserved hypothetical protein
2242856
2243626
3
771


TM.orf2023

hypothetical protein
2243807
2244343
3
537


TM.orf2024
gsiA
putative mureinpeptideoligopeptide ABC
2244340
2246259
1
1920




transporter ATP-binding protein


TM.orf2025
agpA
oligopeptide ABC transporter, periplasmic
2246256
2248238
2
1983




oligopeptide-binding protein


TM.orf2026
appB
oligopeptide ABC transporter, permease
2248337
2249335
3
999




protein


TM.orf2027
appC
putative oligopeptide ABC transporter,
2249332
2250486
1
1155




permease protein


TM.orf2028

CRP/FNR family transcriptional regulator
2251329
2250565
−1
765


TM.orf2029
uppP
undecaprenyl pyrophosphate phosphatase
2251593
2252426
2
834


TM.orf2030
paaF
enoyl-CoA hydratase/isomerase
2252736
2253500
2
765


TM.orf2031

creatininase
2253493
2254263
1
771


TM.orf2032

luciferase family protein
2254407
2255546
2
1140


TM.orf2033

enoyl-CoA hydratase/isomerase
2255575
2256363
1
789


TM.orf2034

AMP-dependent synthetase and ligase
2256491
2258272
3
1782


TM.orf2035
ribB
3,4-dihydroxy-2-butanone 4-phosphate
2258291
2259421
3
1131




synthase


TM.orf2036
ntaB
nitrilotriacetate monooxygenase component B
2259523
2260062
1
540




protein


TM.orf2037
yjjL
major facilitator family transporter
2260395
2261744
2
1350


TM.orf2038
yeaM
putative transcriptional regulator
2262725
2261847
−2
879


TM.orf2039

AsnC-family transcriptional regulator
2262982
2263479
1
498


TM.orf2040
dusA
tRNA-dihydrouridine synthase A
2263678
2264670
1
993


TM.orf2041

putative membrane protein
2264816
2265505
3
690


TM.orf2042

hypothetical protein
2265502
2265876
1
375


TM.orf2043

conserved hypothetical protein
2266046
2267356
3
1311


TM.orf2044

extracellular ligand-binding receptor
2268558
2267443
−1
1116


TM.orf2045

conserved hypothetical protein
2269045
2268692
−3
354


TM.orf2046
IivF
branched-chain amino acid transport system
2269784
2269074
−2
711




ATP-binding protein


TM.orf2047
IivG
ABC transporter component
2270671
2269784
−3
888


TM.orf2048
braE
inner-membrane translocator
2272016
2270691
−2
1326


TM.orf2049
braD
inner-membrane translocator
2272930
2272016
−3
915


TM.orf2050

hypothetical protein
2273700
2273446
−1
255


TM.orf2051
hemA
5-aminolevulinate synthase
2275060
2273834
−3
1227


TM.orf2052

putative transcriptional regulator protein
2275884
2275354
−1
531


TM.orf2053
ygaD
Putative multidrug export ATP-
2278615
2275931
−3
2685




binding/permease protein ygaD


TM.orf2054
estA
Esterase estA
2278937
2281075
3
2139


TM.orf2055

conserved hypothetical protein
2282164
2281118
−3
1047


TM.orf2056
bax
conserved hypothetical protein
2283102
2282185
−1
918


TM.orf2057

hypothetical protein
2283527
2283207
−2
321


TM.orf2058
chvG
sensor histidine kinase
2285247
2283745
−1
1503


TM.orf2059
chvI
transcriptional regulatory protein ChvI
2286110
2285262
−2
849


TM.orf2060

conserved hypothetical protein
2286552
2286094
−1
459


TM.orf2061

hypothetical protein
2287144
2287668
1
525


TM.orf2062

conserved hypothetical protein
2289037
2288207
−3
831


TM.orf2063

ATP-dependent RNA helicase, DEAD
2291518
2289044
−3
2475


TM.orf2064
lig
putative mRNA processing factor
2291671
2292774
1
1104


TM.orf2065
lig
ATP-dependent DNA ligase
2292779
2294395
3
1617


TM.orf2066

conserved hypothetical protein
2294475
2296736
2
2262


TM.orf2067

conserved hypothetical protein
2296920
2297339
2
420


TM.orf2068

Major facilitator superfamily MFS_1
2297336
2298787
3
1452


TM.orf2069

lipoprotein
2298808
2299350
1
543


TM.orf2070
corA
Mg2 transporter protein CorA family protein
2299473
2300498
2
1026


TM.orf2071

hypothetical protein
2300625
2301026
2
402


TM.orf2072
cirA
addiction module killer protein
2301074
2303014
3
1941


TM.orf2073

polyferredoxin
2303035
2304465
1
1431


TM.orf2074

conserved hypothetical protein
2305361
2304483
−2
879


TM.orf2075

FAD dependent oxidoreductase
2305472
2306527
3
1056


TM.orf2076

HD domain protein
2306674
2307246
1
573


TM.orf2077

conserved hypothetical protein
2311926
2307280
−1
4647


TM.orf2078
ytfM
surface antigen
2313988
2311976
−3
2013


TM.orf2079

lipolytic protein G-D-S-L family
2316039
2315095
−1
945


TM.orf2080

opgC protein, require for succinylation of
2317235
2316042
−2
1194




osmoregulated periplasmic glucans


TM.orf2081

oxalate/formate antiporter
2317714
2318352
1
639


TM.orf2082
ybfB
putative MFS transporter
2318417
2318923
3
507


TM.orf2083

hypothetical protein
2318977
2319186
1
210


TM.orf2084
paaJ
DcaF
2320431
2319220
−1
1212


TM.orf2085
paaH
3-hydroxybutyryl-CoA dehydrogenase
2322033
2320468
−1
1566


TM.orf2086
pcaR
transcriptional regulator
2322284
2323099
3
816


TM.orf2087

protein, possibly involved in aromatic
2323156
2323644
1
489




compounds catabolism


TM.orf2088

hypothetical protein
2323701
2323865
2
165


TM.orf2089
serA
putative phosphoglycerate dehydrogenase
2324871
2323915
−1
957




(PGDH), serA-like protein


TM.orf2090
phnE
phosphonate ABC transporter, inner
2325775
2324969
−3
807




membrane subunit


TM.orf2091
phnD
binding protein component of ABC
2326913
2325879
−2
1035




phosphonate transporter


TM.orf2092
phnC
Phosphonates ABC transporter, ATP-binding
2327834
2326980
−2
855




protein


TM.orf2093

conserved hypothetical protein
2328174
2328887
2
714


TM.orf2094
yhaH
Inner membrane protein yhaH
2328978
2329553
2
576


TM.orf2095

guanylate kinase
2330170
2329577
−3
594


TM.orf2096
phnM
phosphonate metabolism protein PhnM
2331288
2330143
−1
1146


TM.orf2097
phnL
Phosphonates transport ATP-binding protein
2332034
2331285
−2
750




phnL


TM.orf2098
phnK
phosphonate C-P lyase system protein PhnK
2332848
2332039
−1
810


TM.orf2099
phnJ
enzyme of phosphonate metabolism
2333783
2332845
−2
939


TM.orf2100
phnI
phosphonate metabolism
2334925
2333780
−3
1146


TM.orf2101
phnH
phosphonate C-P lyase system protein PhnH
2335559
2334930
−2
630


TM.orf2102
phnG
PhnG protein
2336074
2335565
−3
510


TM.orf2103

phosphonate operon transcriptional regulator
2336204
2337016
3
813


TM.orf2104
ppi
peptidyl-prolyl cis-trans isomerase, cyclophilin
2337191
2337766
3
576




type


TM.orf2105

conserved hypothetical protein
2337886
2338764
1
879


TM.orf2106

transcription regulator
2340272
2340514
3
243


TM.orf2107

plasmid stabilization system protein
2340523
2340837
1
315


TM.orf2108
ppi
peptidyl-prolyl cis-trans isomerase cyclophilin
2341092
2341571
2
480




type


TM.orf2109
recQ
ATP-dependent DNA helicase
2341831
2343489
1
1659


TM.orf2110
ylaB
putative signaling membrane protein
2345029
2343524
−3
1506


TM.orf2111

conserved hypothetical protein
2346179
2345247
−2
933


TM.orf2112

patatin
2346754
2347893
1
1140


TM.orf2113
erfK
conserved hypothetical protein
2347890
2349365
2
1476


TM.orf2114

hypothetical protein
2349865
2349590
−3
276


TM.orf2115

phenazine biosynthesis protein PhzF family
2351013
2350081
−1
933


TM.orf2116
ybfL
transposase, is4 family
2352240
2351125
−1
1116


TM.orf2117
yhfA
redox protein
2352467
2352916
3
450


TM.orf2118
ydhC
drug resistance transporter, Bcr/CflA subfamily
2352999
2354153
2
1155




protein


TM.orf2119

conserved hypothetical protein
2354473
2356797
1
2325


TM.orf2120

conserved hypothetical protein
2356794
2357492
2
699


TM.orf2121
cbpA
curved DNA-binding protein
2357752
2358750
1
999


TM.orf2122

glutathione S-transferase
2359462
2358761
−3
702


TM.orf2123

TrkA domain-containing protein
2361260
2359479
−2
1782


TM.orf2124
yfcG
glutathione S-transferase-like protein
2361410
2362114
3
705


TM.orf2125
quiP
peptidase S45 penicillin amidase
2362290
2364647
2
2358


TM.orf2126
siaT
TRAP transporter, DctM-like membrane
2365995
2364673
−1
1323




protein


TM.orf2127

conserved hypothetical protein
2366552
2365992
−2
561


TM.orf2128
dctP
C4-dicarboxylate-binding periplasmic protein
2367655
2366552
−3
1104


TM.orf2129
ygaE
transcriptional regulator
2368507
2367776
−3
732


TM.orf2130

cyclase family protein
2368754
2369530
3
777


TM.orf2131

possible isomerase/decarboxylase
2369581
2370600
1
1020


TM.orf2132
braF
ABC transporter related protein
2370912
2371667
2
756


TM.orf2133
IivF
ABC-type branched-chain amino acid
2371681
2372397
1
717




transport systems, ATPase component


TM.orf2134

Extracellular ligand-binding receptor
2372698
2373918
1
1221


TM.orf2135

branched chain amino acid ABC transporter
2373979
2374866
1
888




permease


TM.orf2136
IivM
ABC-type branched-chain amino acid
2374871
2375854
3
984




transport system, permease component


TM.orf2137
mcp4
Methyl-accepting chemotaxis protein
2376055
2377737
1
1683


TM.orf2138

putative ABC branched-chain amino
2378066
2379283
3
1218




transporter, periplasmic binding protein


TM.orf2139

linocin_M18 bacteriocin protein
2380217
2379390
−2
828


TM.orf2140

conserved hypothetical protein
2380648
2380265
−3
384


TM.orf2141

Zinc transporter
2381604
2380786
−1
819


TM.orf2142

Permeases of the major facilitator superfamily
2382992
2381766
−2
1227


TM.orf2143
ydcO
benzoate transporter subfamily
2384329
2383154
−3
1176


TM.orf2144
mobB
molybdopterin-guanine dinucleotide
2385012
2384446
−1
567




biosynthesis MobB region


TM.orf2145
nrtC
nitrate transporter
2385344
2386798
3
1455


TM.orf2146
nrtB
nitrate ABC transporter, inner membrane
2386816
2387637
1
822




subunit


TM.orf2147
nasD
nitrate ABC transporter, ATPase subunits C
2387665
2388492
1
828




and D


TM.orf2148
nasD
F283498_4 assimilatory nitrite reductase large
2388507
2390984
2
2478




subunit


TM.orf2149
nasE
Nitrite reductase (NAD(P)H) large subunit,
2390999
2391406
3
408




NirD


TM.orf2150
nasA
Anaerobic dehydrogenases, typically
2391403
2393925
1
2523




selenocysteine-containing


TM.orf2151

hypothetical protein
2393951
2394112
3
162


TM.orf2152
cysG
siroheme synthase protein
2394162
2395475
2
1314


TM.orf2153
trpD
Glycosyl transferase, family 3
2395490
2396497
3
1008


TM.orf2154
evgS
two-component sensor histidine kinase
2396494
2398014
1
1521


TM.orf2155
coxL
carbon monoxide dehydrogenase large chain
2398195
2400567
1
2373


TM.orf2156
gltS
sodium/glutamate symporter
2401753
2400554
−3
1200


TM.orf2157
yhhJ
ABC-2 type transporter
2402862
2401750
−1
1113


TM.orf2158
yhiH
ABC transporter permease ATP-binding
2405599
2402870
−3
2730




protein


TM.orf2159
yhiI
HlyD family secretion protein
2406633
2405602
−1
1032


TM.orf2160
envR
TetR family transcriptional regulator
2406898
2407506
1
609


TM.orf2161

conserved hypothetical protein
2407616
2407921
3
306


TM.orf2162

conserved hypothetical protein
2408835
2407939
−1
897


TM.orf2163
yxnA
short chain dehydrogenase
2409110
2410153
3
1044


TM.orf2164

conserved hypothetical protein
2410172
2410669
3
498


TM.orf2165

conserved hypothetical protein
2411430
2410681
−1
750


TM.orf2166

molybdopterin binding domain protein
2412483
2411680
−1
804


TM.orf2167

putative NAD-dependent
2413505
2412480
−2
1026




epimerase/dehydratase


TM.orf2168

sugar fermentation stimulation protein A
2413667
2414458
3
792


TM.orf2169
map
methionine aminopeptidase, type I
2414609
2415403
3
795


TM.orf2170

DNA repair proteins
2415435
2416274
2
840


TM.orf2171
intA
integrase family protein
2416608
2417708
2
1101


TM.orf2172

conserved hypothetical protein
2418398
2418892
3
495


TM.orf2173

conserved hypothetical protein
2419006
2419677
1
672


TM.orf2174

superfamily I DNA/RNA helicase
2419788
2423363
2
3576


TM.orf2175

phosphatidylserine/phosphatidylglycerophosphate/
2423360
2425150
3
1791




cardiolipin synthase-like protein


TM.orf2176

conserved hypothetical protein
2425981
2425283
−3
699


TM.orf2177

conserved hypothetical protein
2426516
2425986
−2
531


TM.orf2178

glutaredoxin
2427986
2427276
−2
711


TM.orf2179

Dps family DNA-binding stress response
2428909
2429463
1
555




protein


TM.orf2180
rhaS
transcriptional regulator, AraC family
2430213
2429470
−1
744


TM.orf2181
yxaF
transcriptional regulator, TetR family
2430962
2430372
−2
591


TM.orf2182
ydeP
HxlR family transcriptional regulator
2431847
2431473
−2
375


TM.orf2183
ywbO
DSBA oxidoreductase
2431963
2432751
1
789


TM.orf2184

conserved hypothetical protein
2432738
2433883
3
1146


TM.orf2185

FAD-dependent pyridine nucleotide-disulphide
2433896
2435191
3
1296




oxidoreductase


TM.orf2186

conserved hypothetical protein
2436402
2436115
−1
288


TM.orf2187
trbI
conjugation TrbI family protein
2437524
2436406
−1
1119


TM.orf2188
trbG
P-type conjugative transfer protein TrbG
2438321
2437593
−2
729


TM.orf2189
trbF
conjugal transfer protein
2439253
2438570
−3
684


TM.orf2190
trbL
P-type conjugative transfer protein TrbL
2440554
2439256
−1
1299


TM.orf2191

P-type conjugative transfer protein TrbJ
2441724
2440960
−1
765


TM.orf2192
trbE
conjugal transfer ATPase TrbE
2444159
2441721
−2
2439


TM.orf2193

type IV secretory pathway VirB3 family protein
2444436
2444164
−1
273


TM.orf2194

conjugal transfer protein TrbC
2444756
2444436
−2
321


TM.orf2195
trbB
conjugal transfer protein trbB
2445673
2444753
−3
921


TM.orf2196

conserved hypothetical protein
2446792
2446364
−3
429


TM.orf2197
traG
TRAG protein
2448802
2446802
−3
2001


TM.orf2198

conserved hypothetical protein
2450722
2448923
−3
1800


TM.orf2199
yjbJ
lytic transglycosylase, catalytic
2451818
2450907
−2
912


TM.orf2200
traF
Peptidase S26, conserved region
2452339
2451821
−3
519


TM.orf2201

conserved hypothetical protein
2452800
2452336
−1
465


TM.orf2202

conserved hypothetical protein
2453042
2452797
−2
246


TM.orf2203

putative partition protein
2453677
2453039
−3
639


TM.orf2204

replication protein A
2454747
2453674
−1
1074


TM.orf2205

conserved hypothetical protein
2455050
2454763
−1
288


TM.orf2206

conserved hypothetical protein
2455516
2455157
−3
360


TM.orf2207

conserved hypothetical protein
2456500
2456216
−3
285


TM.orf2208

XRE family transcriptional regulator
2456842
2456994
1
153


TM.orf2209

SAM-dependent methyltransferase
2457058
2457891
1
834


TM.orf2210

conserved hypothetical protein
2458289
2457966
−2
324


TM.orf2211

DNA polymerase III alpha subunit
2462233
2458967
−3
3267


TM.orf2212
imuB
conserved hypothetical protein
2463621
2462233
−1
1389


TM.orf2213
imuA
conserved hypothetical protein
2464411
2463659
−3
753


TM.orf2214

hypothetical protein
2464755
2464922
2
168


TM.orf2215

conserved hypothetical protein
2465463
2464963
−1
501


TM.orf2216

conserved hypothetical protein
2465757
2465584
−1
174


TM.orf2217
yoaM
conserved hypothetical protein
2466560
2465973
−2
588


TM.orf2218

Gene 61 protein
2468052
2467114
−1
939


TM.orf2219

conserved hypothetical protein
2469420
2468281
−1
1140


TM.orf2220

putative methylase/helicase
2473774
2469431
−3
4344


TM.orf2221

RC187
2474539
2473940
−3
600


TM.orf2222

conserved hypothetical protein
2474999
2474625
−2
375


TM.orf2223

conserved hypothetical protein
2475501
2475010
−1
492


TM.orf2224

conserved hypothetical protein
2476137
2475571
−1
567


TM.orf2225
yubM
ParB domain protein nuclease
2478356
2476200
−2
2157


TM.orf2226

conserved hypothetical protein
2479337
2478537
−2
801


TM.orf2227

putative ardC antirestriction protein
2480466
2479507
−1
960


TM.orf2228

conserved hypothetical protein
2480783
2481640
3
858


TM.orf2229

conserved hypothetical protein
2483151
2481637
−1
1515


TM.orf2230

Domain of unknown function DUF1863
2483227
2483682
1
456


TM.orf2231

conserved hypothetical protein
2483721
2484341
2
621


TM.orf2232

conserved hypothetical protein
2484829
2484362
−3
468


TM.orf2233

conserved hypothetical protein
2485356
2484841
−1
516


TM.orf2234
copB
copper resistance B precursor
2486148
2485456
−1
693


TM.orf2235
pcoA
copper-resistance protein, CopA family
2488406
2486616
−2
1791


TM.orf2236

conserved hypothetical protein
2489421
2488522
−1
900


TM.orf2237

Heavy metal transport/detoxification protein
2489652
2489437
−1
216


TM.orf2238

heavy metal translocating P-type ATPase
2489815
2492364
1
2550


TM.orf2239
nrdH
glutaredoxin
2492427
2492693
2
267


TM.orf2240
ndoR
oxidoreductase FAD-binding subunit
2492708
2493742
3
1035


TM.orf2241

transcriptional regulator, MerR family
2493769
2494266
1
498


TM.orf2242
ydeG
riboflavin synthase subunit alpha
2494328
2495533
3
1206


TM.orf2243

hypothetical protein
2495807
2495577
−2
231


TM.orf2244

conserved hypothetical protein
2496107
2495841
−2
267


TM.orf2245

cation diffusion facilitator family transporter
2497148
2496210
−2
939


TM.orf2246
bepE
hydrophobe/amphiphile efflux-1 (HAE1) family
2500339
2497169
−3
3171




protein


TM.orf2247
acrA
secretion protein HlyD
2501574
2500336
−1
1239


TM.orf2248
ynfL
LysR family transcriptional regulator
2501812
2502717
1
906


TM.orf2249

conserved hypothetical protein
2503076
2503306
3
231


TM.orf2250

DNA repair protein RadC
2503412
2503576
3
165


TM.orf2251
cya2
adenylate cyclase protein
2504733
2503585
−1
1149


TM.orf2252
purB
adenylosuccinate lyase
2505040
2506338
1
1299


TM.orf2253

hypothetical protein
2506873
2506412
−3
462


TM.orf2254

Metallophosphoesterase
2507048
2507887
3
840


TM.orf2255

conserved hypothetical protein
2508288
2507971
−1
318


TM.orf2256

enoyl-[acyl-carrier-protein] reductase (NADH)
2509244
2508462
−2
783


TM.orf2257
purC
phosphoribosylaminoimidazole-
2509598
2510368
3
771




succinocarboxamide synthase


TM.orf2258
yexA
phosphoribosylformylglycinamidine synthase
2510365
2510610
1
246




protein


TM.orf2259
purQ
phosphoribosylformylglycinamidine synthase I
2510648
2511385
3
738


TM.orf2260
purL
Phosphoribosylformylglycinamidine (FGAM)
2511463
2513316
1
1854




synthase, synthetase domain


TM.orf2261
purL
phosphoribosylformylglycinamidine synthase II
2513280
2513669
2
390


TM.orf2262

BolA family protein
2513749
2513985
1
237


TM.orf2263

glutaredoxin-like protein
2514053
2514406
3
354


TM.orf2264

conserved hypothetical protein
2514838
2514488
−3
351


TM.orf2265

PepSY-associated TM helix domain protein
2516373
2514835
−1
1539


TM.orf2266

hypothetical protein
2516633
2516370
−2
264


TM.orf2267
fhuA
TonB-dependent siderophore receptor
2519107
2516627
−3
2481


TM.orf2268
fecR
two component sensor kinase
2520175
2519261
−3
915


TM.orf2269

sigma factor
2520741
2520217
−1
525


TM.orf2270

short chain dehydrogenase
2521530
2520787
−1
744


TM.orf2271
mtaD
5-methylthioadenosine/S-
2521714
2523126
1
1413




adenosylhomocysteine deaminase


TM.orf2272
metC
cystathionine beta-lyase
2524377
2523199
−1
1179


TM.orf2273
glpE
rhodanese-like protein
2526621
2524378
−1
2244


TM.orf2274
pecT
transcriptional regulator, LysR family protein
2526749
2527594
3
846


TM.orf2275

conserved hypothetical protein
2529128
2527611
−2
1518


TM.orf2276

hypothetical protein
2529581
2529147
−2
435


TM.orf2277

conserved hypothetical protein
2530717
2529758
−3
960


TM.orf2278

conserved hypothetical protein
2531797
2531267
−3
531


TM.orf2279
fixN
cbb3-type cytochrome c oxidase subunit I
2532412
2533872
1
1461


TM.orf2280

FixO1 cytochrome C oxidase subunit
2533890
2534651
2
762


TM.orf2281

hypothetical protein
2534648
2534815
3
168


TM.orf2282
petJ
cytochrome-c oxidase fixP chain
2534802
2535671
2
870


TM.orf2283
fixG
cytochrome c oxidase accessory protein CcoG
2535668
2537248
3
1581


TM.orf2284

nitrogen fixation protein fixH
2537335
2537778
1
444


TM.orf2285

hypothetical protein
2537883
2538539
2
657


TM.orf2286

conserved hypothetical protein
2538569
2539285
3
717


TM.orf2287
rpsD
30S ribosomal protein S4
2540006
2539392
−2
615


TM.orf2288
hmuV
ABC transporter related protein
2540874
2540083
−1
792


TM.orf2289

transport system permease protein
2541866
2540871
−2
996


TM.orf2290
yvrC
Fe3+-hydroxamate ABC superfamily ATP
2542804
2541863
−3
942




binding cassette transporter periplasmic family




protein


TM.orf2291
btuB
TonB-dependent receptor
2544963
2542804
−1
2160


TM.orf2292

Methyltransferase type 11
2545817
2545011
−2
807


TM.orf2293
pncA
isochorismatase hydrolase
2547811
2546240
−3
1572


TM.orf2294

alpha-isopropylmalate/homocitrate synthase
2549502
2547865
−1
1638




family transferase


TM.orf2295

hypothetical protein
2549747
2549610
−2
138


TM.orf2296

conserved hypothetical protein
2551506
2550802
−1
705


TM.orf2297
cysS
Cysteinyl-tRNA synthetase
2553762
2552371
−1
1392


TM.orf2298

glutamyl-tRNA synthetase
2555212
2553857
−3
1356


TM.orf2299
nadE
Glutamine-dependent NAD(+) synthetase
2556984
2555293
−1
1692


TM.orf2300

nicotinate phosphoribosyltransferase
2558129
2556999
−2
1131


TM.orf2301

phospho-2-dehydro-3-deoxyheptonate
2559694
2558300
−3
1395




aldolase 1


TM.orf2302
gor
glutathione-disulfide reductase
2561396
2560047
−2
1350


TM.orf2303
gabD
succinic semialdehyde dehydrogenase
2562979
2561489
−3
1491


TM.orf2304

hypothetical protein
2563165
2563524
1
360


TM.orf2305

hypothetical protein
2564350
2564532
1
183


TM.orf2306
FAH
fumarylacetoacetase
2565929
2564673
−2
1257


TM.orf2307
ybiH
transcriptional regulator
2566637
2565945
−2
693


TM.orf2308
mtaD
amidohydrolase
2568043
2566637
−3
1407


TM.orf2309
yiiZ
DctP
2568248
2569267
3
1020


TM.orf2310

tripartite ATP-independent periplasmic
2569304
2569834
3
531




transporter DctQ


TM.orf2311

TRAP dicarboxylate transporter, DctM subunit
2569834
2571129
1
1296


TM.orf2312

cupin 2 domain-containing protein
2571163
2571690
1
528


TM.orf2313
glmS
glucosamine-fructose-6-phosphate
2573607
2571781
−1
1827




aminotransferase


TM.orf2314

conserved hypothetical protein
2575484
2574030
−2
1455


TM.orf2315

protein, phage-related
2578821
2575528
−1
3294


TM.orf2316

conserved hypothetical protein
2579651
2578818
−2
834


TM.orf2317

conserved hypothetical protein
2580302
2579667
−2
636


TM.orf2318

hypothetical protein
2580467
2580315
−2
153


TM.orf2319

hypothetical protein
2581272
2580481
−1
792


TM.orf2320

hypothetical protein
2581716
2581288
−1
429


TM.orf2321

hypothetical protein
2582105
2581716
−2
390


TM.orf2322

conserved hypothetical protein
2582563
2582102
−3
462


TM.orf2323

conserved hypothetical protein
2583563
2582586
−2
978


TM.orf2324

hypothetical protein
2584181
2583597
−2
585


TM.orf2325

conserved hypothetical protein
2585579
2584194
−2
1386


TM.orf2326

putative phage terminase-like protein
2587024
2585618
−3
1407


TM.orf2327
dinB
conserved hypothetical protein
2587742
2587221
−2
522


TM.orf2328

conserved hypothetical protein
2587970
2588917
3
948


TM.orf2329
SRY1
threonine dehydratase
2590188
2588929
−1
1260


TM.orf2330
cvrA
sodium/hydrogen exchanger
2590380
2592305
2
1926


TM.orf2331

conserved hypothetical protein
2592646
2592320
−3
327


TM.orf2332

prevent-host-death protein
2593009
2592758
−3
252


TM.orf2333

fumarylacetoacetat hydroxylase
2593778
2593083
−2
696


TM.orf2334

glycolate reductase
2594922
2593930
−1
993


TM.orf2335
cysG
siroheme synthase
2595157
2595816
1
660


TM.orf2336
cysH
phosphoadenosine phosphosulfate reductase
2595894
2596604
2
711


TM.orf2337
cysQ
3′(2′),5′-bisphosphate nucleotidase
2596698
2597522
2
825


TM.orf2338

hypothetical protein
2597519
2598121
3
603


TM.orf2339
soj
cobyrinic acid a,c-diamide synthase
2598752
2598126
−2
627


TM.orf2340

hypothetical protein
2598950
2599210
3
261


TM.orf2341
ywlC
putative translation factor
2599222
2600265
1
1044


TM.orf2342
pleC
Signal transduction histidine kinase
2600307
2601653
2
1347


TM.orf2343

conserved hypothetical protein
2602071
2601631
−1
441


TM.orf2344

FAD/FMN-containing dehydrogenases
2602148
2603581
3
1434


TM.orf2345

formiminoglutamase
2604410
2603598
−2
813


TM.orf2346
hutH
histidine ammonia-lyase
2605963
2604407
−3
1557


TM.orf2347
mtaD
N-formimino-L-glutamate deiminase
2607327
2605960
−1
1368


TM.orf2348
hutI
imidazolonepropionase
2607424
2608689
1
1266


TM.orf2349
hutC
transcriptional regulator
2608686
2609420
2
735


TM.orf2350

type IV pilus assembly PilZ
2610874
2609423
−3
1452


TM.orf2351
cysM
cysteine synthase
2611161
2612144
2
984


TM.orf2352
ocd
putative ornithine cyclodeaminase
2612141
2613178
3
1038


TM.orf2353

type IV pilus assembly PilZ
2614643
2613186
−2
1458


TM.orf2354
yrhP
Lysine exporter protein (LYSE/YGGA)
2614803
2615441
2
639


TM.orf2355

acetoacetyl-CoA synthetase
2615485
2616360
1
876


TM.orf2356
mcpA
methyl-accepting chemotaxis protein
2618629
2616383
−3
2247


TM.orf2357
cpxA
Sensor protein cpxA
2620146
2618797
−1
1350


TM.orf2358
mtrA
Two-component system response regulator
2620832
2620143
−2
690


TM.orf2359
macA
macrolide efflux protein MacA
2621091
2622275
2
1185


TM.orf2360
macB
ABC transporter related protein
2622280
2624235
1
1956


TM.orf2361

multidrug efflux outer membrane lipoprotein
2624232
2625647
2
1416


TM.orf2362
prtA
hemolysin-type calcium-binding region
2625880
2627820
1
1941


TM.orf2363

glyoxalase/bleomycin resistance
2628245
2627829
−2
417




protein/dioxygenase


TM.orf2364
acnR
transcriptional regulator, TetR family
2628388
2628990
1
603


TM.orf2365

conserved hypothetical protein
2629744
2628974
−3
771


TM.orf2366
fadA
acetyl-CoA acetyltransferase
2629890
2630273
2
384


TM.orf2367

conserved hypothetical protein
2630783
2630322
−2
462


TM.orf2368

conserved hypothetical protein
2631894
2630842
−1
1053


TM.orf2369

conserved hypothetical protein
2632694
2631894
−2
801


TM.orf2370

conserved hypothetical protein
2636857
2632691
−3
4167


TM.orf2371

phage tape measure protein
2643896
2637249
−2
6648


TM.orf2372

conserved hypothetical protein
2644634
2644269
−2
366


TM.orf2373

conserved hypothetical protein
2645607
2644651
−1
957


TM.orf2374

hypothetical protein
2646244
2645810
−3
435


TM.orf2375

conserved hypothetical protein
2646657
2646241
−1
417


TM.orf2376

hypothetical protein
2646902
2646669
−2
234


TM.orf2377

phage capsid protein
2647846
2646902
−3
945


TM.orf2378

hypothetical protein
2648255
2647863
−2
393


TM.orf2379

conserved hypothetical protein
2649469
2648255
−3
1215


TM.orf2380

phage virion morphogenesis protein
2650168
2649653
−3
516


TM.orf2381

putative head morphogenesis protein SPP1
2651240
2650296
−2
945




gp7


TM.orf2382

F, portal protein
2652801
2651527
−1
1275


TM.orf2383

putative phage-related protein
2654573
2653014
−2
1560


TM.orf2384

terminase small subunit
2655147
2654620
−1
528


TM.orf2385

hypothetical protein
2655493
2655170
−3
324


TM.orf2386

conserved hypothetical protein
2655909
2655490
−1
420


TM.orf2387

hypothetical protein
2656112
2655894
−2
219


TM.orf2388

hypothetical protein
2656566
2656105
−1
462


TM.orf2389

conserved hypothetical protein
2657171
2656569
−2
603


TM.orf2390

conserved hypothetical protein
2657702
2657205
−2
498


TM.orf2391

hypothetical protein
2657922
2657707
−1
216


TM.orf2392

Mu-like prophage protein GP16
2658512
2657919
−2
594


TM.orf2393

hypothetical protein
2658720
2658505
−1
216


TM.orf2394

hypothetical protein
2658943
2658713
−3
231


TM.orf2395

hypothetical protein
2659374
2658940
−1
435


TM.orf2396

conserved hypothetical protein
2659622
2659371
−2
252


TM.orf2397

hypothetical protein
2659759
2659619
−3
141


TM.orf2398

hypothetical protein
2660297
2660073
−2
225


TM.orf2399

hypothetical protein
2660522
2660313
−2
210


TM.orf2400

putative transcriptional regulator
2660929
2660519
−3
411


TM.orf2401

phage-related conserved putative protein
2661563
2660922
−2
642


TM.orf2402

phage transposition protein B
2662065
2661565
−1
501


TM.orf2403

hypothetical protein
2662663
2662379
−3
285


TM.orf2404

Transposase-like Mu
2664791
2662674
−2
2118


TM.orf2405

putative cytosolic protein
2665269
2664838
−1
432


TM.orf2406

hypothetical protein
2665798
2665917
1
120


TM.orf2407

XRE family DNA-binding protein
2666075
2666419
3
345


TM.orf2408
fadA
acetyl-CoA acetyltransferase
2666886
2667605
2
720


TM.orf2409

ribonucleotide-diphosphate reductase subunit
2668675
2667572
−3
1104




beta


TM.orf2410

ribonucleotide-diphosphate reductase subunit
2670135
2668681
−1
1455




alpha


TM.orf2411

NrdA protein
2670595
2670113
−3
483


TM.orf2412

XRE family transcriptional regulator
2672385
2670961
−1
1425


TM.orf2413

2-methylcitrate dehydratase
2672553
2674067
2
1515


TM.orf2414
prpC
2-methylcitrate synthase/citrate synthase II
2674090
2675289
1
1200


TM.orf2415
prpB
methylisocitrate lyase
2675307
2676224
2
918


TM.orf2416
metE
5-methyltetrahydropteroyltriglutamate--
2676226
2678535
1
2310




homocysteine S-methyltransferase


TM.orf2417
oatA
acyltransferase 3
2678614
2679765
1
1152


TM.orf2418
asnO
asparagine synthase
2681725
2679773
−3
1953


TM.orf2419

conserved hypothetical protein
2681971
2681738
−3
234


TM.orf2420

hypothetical protein
2682220
2682065
−3
156


TM.orf2421
menE
long-chain-fatty-acid-CoA ligase
2684066
2682306
−2
1761


TM.orf2422

enoyl-CoA hydratase
2685023
2684121
−2
903


TM.orf2423

TetR family transcriptional regulator
2685200
2685826
3
627


TM.orf2424
osmC
OsmC-like protein
2686269
2685838
−1
432


TM.orf2425

conserved hypothetical protein
2686422
2686754
2
333


TM.orf2426

membrane protein
2686744
2687520
1
777


TM.orf2427
ydfF
transcriptional regulator, ArsR family protein
2687572
2688282
1
711


TM.orf2428

putative lantibiotic modifying enzyme
2688293
2691013
3
2721


TM.orf2429

conserved hypothetical protein
2691119
2691400
3
282


TM.orf2430
yjcE
Na+/H+ antiporter
2693071
2691434
−3
1638


TM.orf2431
pobA
4-hydroxybenzoate 3-monooxygenase
2694396
2693212
−1
1185


TM.orf2432

putative 3-carboxy-cis,cis-muconate
2695588
2694389
−3
1200




cycloisomerase (3-carboxymuconate




lactonizing enzyme)


TM.orf2433
pcaG
Protocatechuate 3,4-dioxygenase, alpha
2696165
2695581
−2
585




subunit(PcaG)


TM.orf2434
pcaH
protocatechuate 3,4-dioxygenase, beta
2696874
2696170
−1
705




subunit


TM.orf2435
pcaC
4-carboxymuconolactone decarboxylase
2697284
2696871
−2
414


TM.orf2436
pcaQ
LysR family transcriptional regulator
2697367
2698353
1
987


TM.orf2437
siaT
TRAP transporter, DctM subunit subfamily
2699656
2698319
−3
1338


TM.orf2438

conserved hypothetical protein
2700131
2699661
−2
471


TM.orf2439
siaP
Bacterial extracellular solute-binding protein,
2701242
2700211
−1
1032




family 7


TM.orf2440

conserved hypothetical protein
2701520
2702683
3
1164


TM.orf2441

conserved hypothetical protein
2702696
2703175
3
480


TM.orf2442

conserved hypothetical protein
2709283
2709717
1
435


TM.orf2443

putative acetyltransferase protein
2710251
2709745
−1
507


TM.orf2444
ygeX
diaminopropionate ammonia-lyase
2711459
2710248
−2
1212


TM.orf2445

transcriptional regulator, AsnC family
2711571
2712083
2
513


TM.orf2446

conserved hypothetical protein
2713314
2712058
−1
1257


TM.orf2447

YciI family protein
2713649
2713362
−2
288


TM.orf2448
thyX
thymidylate synthase (FAD)
2714649
2713711
−1
939


TM.orf2449

conserved hypothetical protein
2715206
2715721
3
516


TM.orf2450
ywhH
YbaK/prolyl-tRNA synthetase associated
2715728
2716246
3
519




region


TM.orf2451

conserved hypothetical protein
2716311
2716613
2
303


TM.orf2452

ETC complex I subunit region
2716907
2717200
3
294


TM.orf2453

conserved hypothetical protein
2717483
2718088
3
606


TM.orf2454
visC
conserved hypothetical protein
2719289
2718096
−2
1194


TM.orf2455

putative transmembrane protein
2720401
2719370
−3
1032


TM.orf2456
pecT
LysR family transcriptional regulator
2720510
2721382
3
873


TM.orf2457

conserved hypothetical protein
2721745
2721386
−3
360


TM.orf2458

conserved hypothetical protein
2722102
2721809
−3
294


TM.orf2459
nahR
transcriptional regulator, LysR family
2723159
2722215
−2
945


TM.orf2460
yebQ
major facilitator superfamily MFS_1
2723305
2724750
1
1446


TM.orf2461

conserved hypothetical protein
2725624
2724725
−3
900


TM.orf2462
ycaN
transcriptional regulator, LysR family
2726568
2725621
−1
948


TM.orf2463
yajO
aldo/keto reductase
2726686
2727714
1
1029


TM.orf2464

conserved hypothetical protein
2728262
2727711
−2
552


TM.orf2465

gluconolactonase precursor
2728368
2729294
2
927


TM.orf2466

conserved hypothetical protein
2729352
2729954
2
603


TM.orf2467
yhcX
hydrolase, carbon-nitrogen family protein
2729951
2730880
3
930


TM.orf2468

hypothetical protein
2731163
2730882
−2
282


TM.orf2469

hypothetical protein
2731683
2731336
−1
348


TM.orf2470

conserved hypothetical protein
2732253
2731702
−1
552


TM.orf2471

putative ABC transporter
2732676
2733464
2
789


TM.orf2472

ABC transporter permease protein
2733461
2734240
3
780


TM.orf2473

putative sulfonate/nitrate transport system
2734283
2735254
3
972




substrate-binding protein


TM.orf2474

cation antiporter
2735608
2736081
1
474


TM.orf2475

multiple resistance and pH regulation protein F
2736081
2736404
2
324


TM.orf2476

monovalent cation/proton antiporter,
2736451
2736756
1
306




MnhG/PhaG subunit


TM.orf2477

Na+/H+ antiporter MnhB subunit-related
2736753
2737361
2
609




protein


TM.orf2478

putative monovalent cation/H+ antiporter
2737351
2737776
1
426




subunit B


TM.orf2479
phaC
NADH-ubiquinone oxidoreductase chain 4L
2737778
2738347
3
570


TM.orf2480
nuoN
NADH dehydrogenase (quinone)
2738344
2739831
1
1488


TM.orf2481
nuoN
NADH dehydrogenase (quinone)
2739836
2741353
3
1518


TM.orf2482

hypothetical protein
2741350
2741589
1
240


TM.orf2483
nuoN
putative monovalent cation/H+ antiporter
2741582
2743303
3
1722




subunit D


TM.orf2484
yjaB
acetyltransferase
2743501
2744055
1
555


TM.orf2485

conserved hypothetical protein
2744124
2745497
2
1374


TM.orf2486

transmembrane protein
2745879
2745511
−1
369


TM.orf2487

iron-regulated protein
2746035
2746958
2
924


TM.orf2488
ykvO
short chain dehydrogenase
2747854
2747102
−3
753


TM.orf2489

glutathione S-transferase domain-containing
2748690
2748100
−1
591




protein


TM.orf2490
yqhC
transcriptional regulator protein
2748951
2749880
2
930


TM.orf2491

TetR family transcriptional regulator
2750587
2749901
−3
687


TM.orf2492

hypothetical protein
2750599
2750718
1
120


TM.orf2493

class I peptide chain release factor
2751175
2750732
−3
444


TM.orf2494
mcp4
Methyl-accepting chemotaxis protein
2753444
2751255
−2
2190


TM.orf2495
dcrA
methyl-accepting chemotaxis protein
2753759
2755423
3
1665


TM.orf2496
htpX
peptidase M48 Ste24p
2756252
2755389
−2
864


TM.orf2497

hypothetical protein
2757400
2756330
−3
1071


TM.orf2498
ahpC
alkylhydroperoxide reductase C
2757718
2758257
1
540


TM.orf2499
ahpD
AhpD, alkyl hydroperoxide reductase D
2758548
2759081
2
534


TM.orf2500
asnB
asparagine synthase (glutamine-hydrolyzing)
2759614
2761425
1
1812


TM.orf2501
cphA
GNAT-family acetyltransferase TIGR03103
2761433
2763292
3
1860


TM.orf2502
yhfE
peptidase M42 family hydrolase
2763289
2764512
1
1224


TM.orf2503
cysB
cysteine synthase
2764722
2765726
2
1005


TM.orf2504

Alanyl-tRNA editing protein alaX-M
2765778
2766548
2
771


TM.orf2505

alkyl hydroperoxide reductase/Thiol specific
2767763
2766549
−2
1215




antioxidant/Mal allergen


TM.orf2506
MPST
rhodanese-related sulfurtransferase
2768043
2768897
2
855


TM.orf2507

putative acetyltransferase
2769196
2769819
1
624


TM.orf2508
ooxA
BFD/(2Fe—2S)-binding domain-containing
2769816
2771240
2
1425




protein


TM.orf2509

hypothetical protein
2771237
2772184
3
948


TM.orf2510

alpha/beta hydrolase fold
2773161
2772196
−1
966


TM.orf2511
aapP
ABC transporter component
2774099
2773287
−2
813


TM.orf2512
aapM
general L-amino acid transport system
2775219
2774116
−1
1104




permease protein


TM.orf2513
yhdX
putative amino acid ABC transporter
2776429
2775224
−3
1206




permease protein


TM.orf2514
aapJ
general L-amino acid transport system
2777638
2776607
−3
1032




substrate-binding protein


TM.orf2515
metC
cystathionine beta-lyase
2777991
2779223
2
1233


TM.orf2516

L-sorbosone dehydrogenase
2779267
2780544
1
1278


TM.orf2517

hypothetical protein
2780657
2782402
3
1746


TM.orf2518
luxO
two-component system response regulator
2783883
2782414
−1
1470




LuxO


TM.orf2519

FOG: TPR repeat, SEL1 subfamily
2784735
2784028
−1
708


TM.orf2520

O-antigen polymerase
2786030
2784870
−2
1161


TM.orf2521

hypothetical protein
2787184
2786264
−3
921


TM.orf2522

putative DNA helicase related protein
2790071
2787465
−2
2607


TM.orf2523

putative DNA helicase related protein
2793105
2790073
−1
3033


TM.orf2524
oxyR
transcriptional regulator, LysR family
2793327
2794232
2
906


TM.orf2525
yagI
putative transcriptional regulator
2795001
2794255
−1
747


TM.orf2526

TRAP dicarboxylate transporter, DctM subunit
2796343
2795048
−3
1296


TM.orf2527

Tripartite ATP-independent periplasmic
2796936
2796388
−1
549




transporter DctQ component


TM.orf2528
yiiZ
TRAP dicarboxylate transporter- DctP subunit
2797997
2796951
−2
1047


TM.orf2529

Enoyl-CoA hydratase/isomerase
2798877
2798095
−1
783


TM.orf2530
paaG
enoyl-CoA hydratase/isomerase
2799231
2800055
2
825


TM.orf2531

L-carnitine dehydratase
2800069
2800272
1
204


TM.orf2532
yfdE
L-carnitine dehydratase/bile acid-inducible
2800269
2801213
2
945




protein F


TM.orf2533
ydcS
extracellular solute-binding protein family 1
2801396
2802571
3
1176


TM.orf2534
ydcT
ABC transporter related protein
2802653
2803717
3
1065


TM.orf2535
ydcU
binding-protein-dependent transport systems
2803714
2804667
1
954




inner membrane component


TM.orf2536
ydcV
binding-protein-dependent transport systems
2804657
2805469
3
813




inner membrane component


TM.orf2537

Aminobutyraldehyde dehydrogenase
2805481
2806926
1
1446


TM.orf2538

short chain dehydrogenase
2807565
2806969
−1
597


TM.orf2539
yusZ
short chain dehydrogenase
2807641
2808489
1
849


TM.orf2540
tuaC
glycosyl transferase, group 1 family protein
2809650
2808493
−1
1158


TM.orf2541
glmU
UDP-N-acetylglucosamine pyrophosphorylase
2809869
2811233
2
1365


TM.orf2542

amino acid carrier protein
2812699
2811248
−3
1452


TM.orf2543

auxin efflux carrier
2812835
2813761
3
927


TM.orf2544
yvfR
ABC transporter ATP-binding protein
2813832
2814833
2
1002


TM.orf2545

ABC-2 type transporter
2814833
2815645
3
813


TM.orf2546
pyrB
Aspartate carbamoyltransferase
2815807
2816742
1
936


TM.orf2547
pyrC
dihydroorotase-like protein
2816827
2818170
1
1344


TM.orf2548
plsY
Glycerol-3-phosphate acyltransferase
2818222
2818857
1
636


TM.orf2549
ppsD
AMP-dependent synthetase and ligase
2821610
2818842
−2
2769


TM.orf2550

conserved hypothetical protein
2822581
2821583
−3
999


TM.orf2551
smf
Predicted Rossmann fold nucleotide-binding
2822778
2823923
2
1146




protein involved in DNA uptake


TM.orf2552
topA
DNA topoisomerase I
2824023
2826851
2
2829


TM.orf2553
rnr
RNAse R
2827044
2829389
2
2346


TM.orf2554
garL
putative HpcH/HpaI aldolase family protein
2829389
2830159
3
771


TM.orf2555
bcr
Bcr/CflA subfamily drug resistance transporter
2830184
2831434
3
1251


TM.orf2556
fpr
Oxidoreductase FAD-binding domain protein
2831561
2832334
3
774


TM.orf2557

hypothetical protein
2832962
2832366
−2
597


TM.orf2558
rpmG
Ribosomal protein L33
2833278
2833445
2
168


TM.orf2559
pleD
response regulator With diguanylate cyclase
2834972
2833596
−2
1377




(GGDEF) domain


TM.orf2560
divK
response regulator receiver protein
2835344
2834979
−2
366


TM.orf2561
mak
putative kinase (mak-like)/transcriptional
2835614
2836603
3
990




regulator, actin-like ATPase domain




(NagC/XylR (ROK) familiy)


TM.orf2562

ImpB/MucB/SamB family
2836817
2837962
3
1146


TM.orf2563

Putative esterase
2838395
2837967
−2
429


TM.orf2564

conserved hypothetical protein
2839379
2838450
−2
930


TM.orf2565
CPA
hydrolase, carbon-nitrogen family
2840387
2839506
−2
882


TM.orf2566

membrane protein involved in aromatic
2840714
2842075
3
1362




hydrocarbon degradation


TM.orf2567
aguA
agmatine deiminase
2843195
2842119
−2
1077


TM.orf2568
ackA
acetate kinase
2844673
2843495
−3
1179


TM.orf2569
pta
bifunctional enoyl-CoA hydratase/phosphate
2846073
2844670
−1
1404




acetyltransferase


TM.orf2570
ldh
leucine dehydrogenase
2847277
2846210
−3
1068


TM.orf2571
yybA
transcriptional regulator
2848082
2847513
−2
570


TM.orf2572

sodium/sulphate symporter
2849593
2848193
−3
1401


TM.orf2573

3-methylcrotonoyl-CoA carboxylase subunit
2852581
2849621
−3
2961




alpha


TM.orf2574
paaF
enoyl-CoA hydratase/isomerase
2853442
2852645
−3
798


TM.orf2575

carboxyltransferase subunit of acetyl-CoA
2855110
2853503
−3
1608




carboxylase


TM.orf2576
doxJ
DSBA oxidoreductase
2855841
2855239
−1
603


TM.orf2577
mmgC
acyl-CoA dehydrogenase
2857054
2855921
−3
1134


TM.orf2578

acetyl-CoA acetyltransferase
2858438
2857245
−2
1194


TM.orf2579
IVD2
isovaleryl-CoA dehydrogenase
2859703
2858534
−3
1170


TM.orf2580

thioesterase superfamily protein
2860250
2859762
−2
489


TM.orf2581
oxyR
LysR family transcriptional regulator
2860607
2861512
3
906


TM.orf2582

conserved hypothetical protein
2861863
2861534
−3
330


TM.orf2583

AraC family transcriptional regulator
2862942
2861932
−1
1011


TM.orf2584

ThiJ/PfpI domain protein
2863077
2863775
2
699


TM.orf2585

transporter
2863873
2865057
1
1185


TM.orf2586
fecI
sigma-24 (FecI)
2865141
2865656
2
516


TM.orf2587
fecR
two component sensor histidine kinase
2865745
2866692
1
948




FecR/PupR


TM.orf2588
bhuA
TonB-dependent receptor
2866810
2869065
1
2256


TM.orf2589

conserved hypothetical protein
2869604
2869125
−2
480


TM.orf2590
exoZ
acyltransferase 3
2869734
2870780
2
1047


TM.orf2591

divalent cation transporter
2872171
2870762
−3
1410


TM.orf2592
maeB
malic enzyme
2874567
2872258
−1
2310


TM.orf2593
NpB
lipoate-protein ligase B
2874843
2875538
2
696


TM.orf2594
bioY
putative biotin transporter bioY
2876104
2875556
−3
549


TM.orf2595

conserved hypothetical protein
2876700
2876224
−1
477


TM.orf2596
acyP
acylphosphatase
2877104
2876739
−2
366


TM.orf2597
DLD2
FAD linked oxidase domain protein
2878610
2877171
−2
1440


TM.orf2598
Pcca
propionyl-CoA carboxylase alpha chain
2880804
2878822
−1
1983


TM.orf2599

hypothetical protein
2881093
2880827
−3
267


TM.orf2600
PCCB
propionyl-CoA carboxylase beta chain
2882666
2881134
−2
1533


TM.orf2601

ATP synthase mitochondrial F1 complex
2883679
2882942
−3
738




assembly factor 2


TM.orf2602

hydrolase, haloacid dehalogenase-like family
2884392
2883676
−1
717




protein


TM.orf2603
rluC
ribosomal large subunit pseudouridine
2885369
2884389
−2
981




synthase C


TM.orf2604
crcB
crcB protein
2885761
2885366
−3
396


TM.orf2605
rarA
recombination factor protein RarA
2887095
2885758
−1
1338


TM.orf2606
degP
periplasmic serine protease, Do
2888628
2887156
−1
1473


TM.orf2607
rplQ
50S ribosomal protein L17
2889227
2888808
−2
420


TM.orf2608
rpoA
DNA-directed RNA polymerase subunit alpha
2890401
2889385
−1
1017


TM.orf2609
rpsK
ribosomal protein S11
2890992
2890600
−1
393


TM.orf2610
rpsM
ribosomal protein S13
2891370
2891002
−1
369


TM.orf2611
adk
adenylate kinase
2892128
2891475
−2
654


TM.orf2612
secY
preprotein translocase, SecY subunit
2893468
2892125
−3
1344


TM.orf2613
rplO
ribosomal protein L15
2894180
2893698
−2
483


TM.orf2614
rpmD
50S ribosomal protein L30
2894431
2894237
−3
195


TM.orf2615
rpsE
small subunit ribosomal protein S5
2895000
2894437
−1
564


TM.orf2616
rplR
ribosomal protein L18
2895373
2895014
−3
360


TM.orf2617
rplF
large subunit ribosomal protein L6
2895921
2895388
−1
534


TM.orf2618
rpsH
30S ribosomal protein S8
2896331
2895933
−2
399


TM.orf2619
rpsN
small subunit ribosomal protein S14
2896653
2896348
−1
306


TM.orf2620
rplE
50S ribosomal protein L5
2897214
2896675
−1
540


TM.orf2621
rplX
ribosomal protein L24
2897549
2897232
−2
318


TM.orf2622
rplN
large subunit ribosomal protein L14
2897917
2897549
−3
369


TM.orf2623
rpsQ
ribosomal protein S17
2898186
2897950
−1
237


TM.orf2624
rpmC
50S ribosomal protein L29
2898394
2898191
−3
204


TM.orf2625
rplP
50S ribosomal protein L16
2898818
2898399
−2
420


TM.orf2626
rpsC
30S ribosomal protein S3
2899534
2898857
−3
678


TM.orf2627
rplV
ribosomal protein L22
2899914
2899534
−1
381


TM.orf2628
rpsS
small subunit ribosomal protein S19
2900195
2899917
−2
279


TM.orf2629
rplB
Ribosomal protein L2
2901038
2900214
−2
825


TM.orf2630
rplW
Ribosomal protein L23
2901336
2901043
−1
294


TM.orf2631
rplD
50S ribosomal protein L4P
2901956
2901333
−2
624


TM.orf2632
rplC
ribosomal protein L3
2902644
2901967
−1
678


TM.orf2633
rpsJ
Ribosomal protein S10
2903003
2902692
−2
312


TM.orf2634
tuf1
Translation elongation factor Tu
2904382
2903192
−3
1191


TM.orf2635
fusA
elongation factor Tu
2906518
2904437
−3
2082


TM.orf2636
rpsG
30S ribosomal protein S7
2907033
2906563
−1
471


TM.orf2637
rpsL
30S ribosomal protein S12
2907431
2907060
−2
372


TM.orf2638
rpoC
DNA-directed RNA polymerase subunit beta′
2912179
2907944
−3
4236


TM.orf2639
rpoB
DNA-directed RNA polymerase subunit beta
2916387
2912299
−1
4089


TM.orf2640

hypothetical protein
2916691
2916506
−3
186


TM.orf2641
rplL
large subunit ribosomal protein L7/L12
2917501
2917124
−3
378


TM.orf2642
rplJ
50S ribosomal protein L10
2918161
2917643
−3
519


TM.orf2643
rplA
ribosomal protein L1
2919219
2918500
−1
720


TM.orf2644
rplK
50S ribosomal protein L11
2919652
2919224
−3
429


TM.orf2645
nusG
transcription antitermination protein NusG
2920374
2919841
−1
534


TM.orf2646

preprotein translocase subunit SecE
2920593
2920396
−1
198


TM.orf2647
tuf1
Translation elongation factor Tu
2922459
2921305
−1
1155


TM.orf2648
rlmB
23S rRNA (guanosine-2′-O-)-
2923055
2923909
3
855




methyltransferase


TM.orf2649
mtrB
two-component sensor histidine kinase
2925366
2923870
−1
1497


TM.orf2650
cusR
Response regulator consisting of a CheY-like
2926069
2925353
−3
717




receiver domain and a winged-helix DNA-




binding domain


TM.orf2651
degP
putative serine protease do-like
2927881
2926325
−3
1557


TM.orf2652

biotin sulfoxide reductase
2928767
2931172
3
2406


TM.orf2653
atm1
Iron-sulfur clusters transporter
2931181
2933037
1
1857


TM.orf2654

hypothetical protein
2933034
2933621
2
588


TM.orf2655

hypothetical protein
2933808
2933984
2
177


TM.orf2656
yyaM
transporter yyaM
2934182
2935126
3
945


TM.orf2657
CRY2
Deoxyribodipyrimidine photolyase
2935239
2936708
2
1470


TM.orf2658

amine oxidase
2936714
2938120
3
1407


TM.orf2659

DUF1365 domain containing protein
2938216
2938983
1
768


TM.orf2660

Cyclopropane-fatty-acyl-phospholipid synthase
2938980
2940230
2
1251


TM.orf2661

Short-chain dehydrogenase/reductase SDR
2940220
2941020
1
801


TM.orf2662

conserved hypothetical protein
2941480
2941040
−3
441


TM.orf2663

deoxyribodipyrimidine photolyase-related
2943093
2941477
−1
1617




protein


TM.orf2664

Dehydrogenases with different specificities
2943321
2943100
−1
222




(related to short-chain alcohol




dehydrogenases)


TM.orf2665
silC
RND efflux system outer membrane
2945018
2943492
−2
1527




lipoprotein


TM.orf2666
bepE
hydrophobe/amphiphile efflux-1 (HAE1) family
2948190
2945038
−1
3153




protein


TM.orf2667
bepD
multidrug-efflux system secretion protein
2949383
2948187
−2
1197


TM.orf2668
bepR
transcription regulator protein
2949681
2950376
2
696


TM.orf2669

mutator insertion sequence transposase
2950603
2950968
1
366




protein


TM.orf2670

conserved hypothetical protein
2951085
2952566
2
1482


TM.orf2671

conserved hypothetical protein
2952556
2954118
1
1563


TM.orf2672

Dehydrogenase
2956176
2954548
−1
1629


TM.orf2673

DNA polymerase bacteriophage-type
2956396
2957304
1
909


TM.orf2674
slt
Soluble lytic murein transglycosylase and
2957490
2959313
2
1824




related regulatory protein


TM.orf2675
exbB
tonB-system energizer ExbB
2959543
2960499
1
957


TM.orf2676
exbD
biopolymer transport protein
2960504
2960962
3
459


TM.orf2677
tonB
TonB family protein
2960959
2961804
1
846


TM.orf2678
moaB
molybdenum cofactor biosynthesis protein B
2961881
2962420
3
540


TM.orf2679

radical SAM domain protein
2962496
2963704
3
1209


TM.orf2680
arnT
Undecaprenyl phosphate-alpha-4-amino-4-
2965367
2963712
−2
1656




deoxy-L-arabinose arabinosyl transferase


TM.orf2681
lpxB
Lipid-A-disaccharide synthase
2965708
2965364
−3
345


TM.orf2682
arnC
glycosyl transferase family 2
2966607
2965705
−1
903


TM.orf2683
qseC
periplasmic sensor signal transduction
2968219
2966765
−3
1455




histidine kinase


TM.orf2684
mprA
response regulator
2968910
2968206
−2
705


TM.orf2685
nudC
MutT/NUDIX family protein
2969908
2968943
−3
966


TM.orf2686
dnaX
DNA polymerase III subunit gamma/tau
2970289
2972370
1
2082


TM.orf2687

transcriptional regulator
2972469
2972792
2
324


TM.orf2688

AraC family transcriptional regulator
2972809
2973615
1
807


TM.orf2689

glyoxalase/bleomycin resistance
2973643
2974044
1
402




protein/dioxygenase


TM.orf2690
cca
polyA polymerase related protein
2974125
2975264
2
1140


TM.orf2691

Ferritin, Dps family protein
2975445
2975948
2
504


TM.orf2692

HIT family protein
2976492
2975956
−1
537


TM.orf2693
recR
Recombinational DNA repair protein (RecF
2976645
2977244
2
600




pathway)


TM.orf2694

UDP-glucose: sterol glucosyltransferase
2977261
2978571
1
1311


TM.orf2695

putative signal peptide
2979704
2978574
−2
1131


TM.orf2696

hypothetical protein
2981781
2980027
−1
1755


TM.orf2697
rmuC
DNA recombination protein rmuC homolog
2983064
2981907
−2
1158


TM.orf2698
def
N-formylmethionyl-tRNA deformylase
2983264
2983818
1
555


TM.orf2699
fmt
methionyl-tRNA formyltransferase fmt
2983861
2984829
1
969


TM.orf2700

PepSY-associated TM helix
2987044
2985575
−3
1470


TM.orf2701
fhuA
ferrichrome-iron receptor
2989523
2987049
−2
2475


TM.orf2702
fecR
putative FecR
2990587
2989607
−3
981


TM.orf2703
fecI
sigma factor
2991125
2990580
−2
546


TM.orf2704

hypothetical protein
2991823
2991218
−3
606


TM.orf2705
dapE
succinyl-diaminopimelate desuccinylase
2993001
2991820
−1
1182


TM.orf2706
dapD
Tetrahydrodipicolinate N-succinyltransferase
2993918
2993052
−2
867


TM.orf2707

putative hydrolase
2994760
2993993
−3
768


TM.orf2708
yhjK
signal transduction protein
2994917
2996593
3
1677


TM.orf2709
dcrA
chemotaxis sensory transducer
2996892
2998247
2
1356


TM.orf2710
argB
acetylglutamate kinase
2999261
2998344
−2
918


TM.orf2711
engB
ribosome biogenesis GTP-binding protein
3000104
2999439
−2
666




YsxC


TM.orf2712
oxaA
60 kDa inner membrane insertion protein
3001983
3000277
−1
1707


TM.orf2713

conserved hypothetical protein
3002392
3001976
−3
417


TM.orf2714
rnpA
Bacterial ribonuclease P protein
3002886
3002389
−1
498


TM.orf2715
rpmH
50S ribosomal protein L34
3003070
3002936
−3
135


TM.orf2716

hypothetical protein
3003765
3003271
−1
495


TM.orf2717
ctpF
ATPase, E1-E2 type
3003941
3006679
3
2739


TM.orf2718
ydjX
mercuric reductase
3006788
3007558
3
771


TM.orf2719
merA
pyridine nucleotide-disulphide oxidoreductase
3007555
3009015
1
1461




dimerisation region


TM.orf2720
yycG
periplasmic sensor signal transduction
3009034
3010491
1
1458




histidine kinase


TM.orf2721
mdmC
O-methyltransferase mdmC
3011203
3010514
−3
690


TM.orf2722
ygiF
conserved hypothetical protein
3012925
3011234
−3
1692


TM.orf2723
gsiA
ABC transporter, nucleotide binding/ATPase
3013258
3015117
1
1860




protein


TM.orf2724
gsiB
extracellular solute-binding protein
3015235
3016773
1
1539


TM.orf2725
gsiC
binding-protein-dependent transport systems
3017180
3018103
3
924




inner membrane component


TM.orf2726
appC
ABC transporter, membrane spanning protein
3018116
3018961
3
846




(oligopeptide)


TM.orf2727

conserved hypothetical protein
3018999
3020756
2
1758


TM.orf2728

ABC transporter related protein
3021633
3020782
−1
852


TM.orf2729
cmpB
sulfate ester transporter permease protein
3022382
3021633
−2
750


TM.orf2730
ssuC
sulfate ester transport system permease
3023287
3022439
−3
849




protein


TM.orf2731
ssuA
sulfate ester transport system substrate-
3024407
3023304
−2
1104




binding protein


TM.orf2732

conserved hypothetical protein
3025963
3024413
−3
1551


TM.orf2733
gcvA
LysR family transcriptional regulator
3026142
3027077
2
936


TM.orf2734

iron ABC transporter substrate binding protein
3027174
3028073
2
900


TM.orf2735
yfeB
ABC transporter related protein
3028094
3029008
3
915


TM.orf2736
yfeC
Chelated iron transport system membrane
3029005
3029865
1
861




protein yfeC


TM.orf2737
yfeD
Chelated iron transport system membrane
3029862
3030698
2
837




protein yfeD


TM.orf2738
narP
DNA-binding response regulator
3030785
3031390
3
606


TM.orf2739
envZ
signal transduction histidine kinase
3031393
3033165
1
1773


TM.orf2740

hypothetical protein
3033317
3034462
3
1146


TM.orf2741

conserved hypothetical protein
3034706
3034455
−2
252


TM.orf2742

conserved hypothetical protein
3035290
3034970
−3
321


TM.orf2743
lysS
lysyl-tRNA synthetase, class I
3037119
3035458
−1
1662


TM.orf2744
yoaA
ATP-dependent DNA helicase
3037259
3040222
3
2964


TM.orf2745

conserved hypothetical protein
3040305
3040676
2
372


TM.orf2746
rnhB
Ribonuclease HII
3040806
3041465
2
660


TM.orf2747
ccrM
modification methylase
3041462
3042709
3
1248


TM.orf2748

Tetratricopeptide repeat protein
3044104
3042713
−3
1392


TM.orf2749
yfhQ
A/G-specific adenine glycosylase
3045424
3044249
−3
1176


TM.orf2750
gcvA
LysR family transcriptional regulator
3046455
3045505
−1
951


TM.orf2751

hypothetical protein
3046612
3046824
1
213


TM.orf2752
yddQ
isochorismatase family protein
3046859
3047482
3
624


TM.orf2753

YCII-related
3047586
3048008
2
423


TM.orf2754

RNA polymerase sigma-70 factor, ECF family
3047989
3049269
1
1281




protein


TM.orf2755

Zn-ribbon-containing, possibly RNA-binding
3049341
3049919
2
579




protein and truncated derivatives


TM.orf2756

DsbA oxidoreductase
3050099
3050797
3
699


TM.orf2757
smc
chromosome segregation ATPase
3050825
3055081
3
4257


TM.orf2758
cobO
cob(I)alamin adenosyltransferase
3056281
3055619
−3
663


TM.orf2759
cobN
cobaltochelatase
3060072
3056278
−1
3795


TM.orf2760
cobW
cobalamin synthesis protein cobW
3061138
3060086
−3
1053


TM.orf2761
btuB
receptor
3061646
3063613
3
1968


TM.orf2762
fdx4
Ferredoxin, 2Fe—2S
3063597
3064358
2
762


TM.orf2763

periplasmic binding protein
3064355
3065317
3
963


TM.orf2764
yjbI
protozoan/cyanobacterial globin family protein
3065328
3065810
2
483


TM.orf2765

putative transcriptional regulator
3066493
3065927
−3
567


TM.orf2766
rhtA
Inner membrane transporter rhtA
3066596
3067492
3
897


TM.orf2767
cobB
Cobyrinic acid a,c-diamide synthase CbiA
3068814
3067474
−1
1341


TM.orf2768
cobA
putative uroporphyrin-III C-methyltransferase
3069710
3068811
−2
900




protein


TM.orf2769
cbiD
cobalt-precorrin-6A synthase
3069779
3070960
3
1182


TM.orf2770
cobK
precorrin-6x reductase
3070965
3071759
2
795


TM.orf2771
hupE
HupE/UreJ protein
3072327
3071746
−1
582


TM.orf2772
cobM
precorrin-4 C11-methyltransferase
3073264
3072419
−3
846


TM.orf2773
cobJ
precorrin-3B C17-methyltransferase (cobJ)
3075147
3073261
−1
1887


TM.orf2774
cobI
precorrin 2 methylase
3075887
3075144
−2
744


TM.orf2775
cobL
Uroporphyrin-III C/tetrapyrrole
3077104
3075866
−3
1239




(Corrin/Porphyrin) methyltransferase


TM.orf2776
cobH
Precorrin-8X methylmutase
3077739
3077101
−1
639


TM.orf2777
cbiX
Sirohydrochlorin cobaltochelatase
3078893
3077736
−2
1158


TM.orf2778
atpI
ATP synthase protein I
3079616
3080020
3
405


TM.orf2779
atpB
F0F1-type ATP synthase, subunit a
3080135
3080866
3
732


TM.orf2780
atpE
ATP synthase C chain
3080986
3081213
1
228


TM.orf2781

H + transporting two-sector ATPase B/B′
3081431
3081934
3
504




subunit


TM.orf2782
atpF
ATP synthase B chain precursor
3081940
3082422
1
483


TM.orf2783
icfG
protein serine/threonine phosphatase
3082791
3084833
2
2043


TM.orf2784

ABC-type transport system, periplasmic
3084830
3086056
3
1227




component


TM.orf2785

conserved hypothetical protein
3087609
3086035
−1
1575


TM.orf2786

putative inner membrane transport protein
3089987
3087606
−2
2382


TM.orf2787

glycine dehydrogenase subunit 2
3091764
3090199
−1
1566


TM.orf2788

glycine dehydrogenase subunit 1
3093125
3091761
−2
1365


TM.orf2789
gcvH
glycine cleavage system H protein
3093510
3093133
−1
378


TM.orf2790
gcvT
glycine cleavage system T protein
3094692
3093553
−1
1140


TM.orf2791

conserved hypothetical protein
3095752
3095186
−3
567


TM.orf2792

CDGSH iron sulfur domain-containing protein 3
3096209
3095940
−2
270


TM.orf2793
ispH
4-hydroxy-3-methylbut-2-enyl diphosphate
3096503
3097483
3
981




reductase


TM.orf2794
thrB
Homoserine kinase ThrB
3097567
3098532
1
966


TM.orf2795
rnhA
ribonuclease H
3098519
3098974
3
456


TM.orf2796
upp
uracil phosphoribosyltransferase
3099673
3098999
−3
675


TM.orf2797

Redoxin domain protein
3100282
3099800
−3
483


TM.orf2798
dsbD
Thiol: disulfide interchange protein
3102643
3100418
−3
2226


TM.orf2799

transcriptional regulator
3102915
3103508
2
594


TM.orf2800

conserved hypothetical protein
3104602
3103775
−3
828


TM.orf2801
rimJ
GCN5-related N-acetyltransferase
3105314
3104664
−2
651


TM.orf2802
tusA
SirA-like protein
3105687
3105343
−1
345


TM.orf2803

M16 family peptidase
3107003
3105726
−2
1278


TM.orf2804
thrC
threonine synthase
3108468
3107065
−1
1404


TM.orf2805

Thermostable carboxypeptidase 1
3110179
3108641
−3
1539


TM.orf2806
ctaA
cytochrome C oxidase assembly protein, AA3
3111219
3110215
−1
1005




subunit-controlling protein


TM.orf2807

SURF1 family protein
3111981
3111298
−1
684


TM.orf2808

conserved hypothetical protein
3112341
3112093
−1
249


TM.orf2809

putative cytochrome c oxidase polypeptide III
3113309
3112500
−2
810




(cytochrome aa3 subunit 3)


TM.orf2810
ctaG
cytochrome-c oxidase assembly protein
3114153
3113482
−1
672


TM.orf2811

hypothetical protein
3114413
3114198
−2
216


TM.orf2812
ctaB
putative protoheme IX farnesyltransferase
3115450
3114410
−3
1041




(heme o synthase)


TM.orf2813
ctaD
cytochrome c oxidase, subunit I
3117072
3115573
−1
1500


TM.orf2814
ctaC
cytochrome C oxidase subunit II
3118046
3117207
−2
840


TM.orf2815

peptidase U62 modulator of DNA gyrase
3118594
3120039
1
1446


TM.orf2816

TRAP transporter, 4TM/12TM fusion protein
3122292
3120118
−1
2175


TM.orf2817

TRAP transporter solute receptor, TAXI family
3123381
3122419
−1
963


TM.orf2818

conserved hypothetical protein
3124273
3123434
−3
840


TM.orf2819
yfhH
RpiR family transcriptional regulator
3125326
3124340
−3
987


TM.orf2820

aminotransferase class-III
3126719
3125340
−2
1380


TM.orf2821

putative glutamatecysteine ligase
3128133
3126892
−1
1242


TM.orf2822
rsmE
ribosomal RNA small subunit
3129106
3128333
−3
774




methyltransferase


TM.orf2823
Coq2
4-hydroxybenzoate polyprenyltransferase and
3129254
3130210
3
957




related prenyltransferases


TM.orf2824

hypothetical protein
3130457
3130176
−2
282


TM.orf2825
ubiD
3-octaprenyl-4-hydroxybenzoate carboxy-
3130544
3132064
3
1521




lyase


TM.orf2826

conserved hypothetical protein
3132143
3133906
3
1764


TM.orf2827

hypothetical protein
3133963
3134148
1
186


TM.orf2828

metallophosphoesterase
3134264
3135094
3
831


TM.orf2829
pmbA
Peptidase U62, modulator of DNA gyrase
3135206
3136573
3
1368


TM.orf2830
suhB
inositol-phosphate phosphatase
3136627
3137493
1
867


TM.orf2831
msbA
ABC-type multidrug transport system, ATPase
3137447
3139429
3
1983




and permease components


TM.orf2832

conserved hypothetical protein
3139435
3140166
1
732


TM.orf2833
waaA
three-deoxy-D-manno-octulosonic-acid
3140163
3141497
2
1335




transferase


TM.orf2834
lpxK
Tetraacyldisaccharide 4′-kinase
3141494
3142582
3
1089


TM.orf2835

Lauroyl/myristoyl acyltransferase
3142579
3143499
1
921


TM.orf2836

hypothetical protein
3144007
3143807
−3
201


TM.orf2837

hypothetical protein
3144272
3144619
3
348


TM.orf2838

Putative amidase
3146122
3144731
−3
1392


TM.orf2877

cytidine/deoxycytidylate deaminase, zinc-
3185617
3185183
−3
435




binding region


TM.orf2878

possible gamma-glutamyl-gamma-
3185753
3186490
3
738




aminobutyrate hydrolase


TM.orf2879

pseudouridine synthase, Rsu
3186487
3188253
1
1767


TM.orf2880

N6-adenine-specific methylase
3188260
3188895
1
636


TM.orf2881

conserved hypothetical protein
3188997
3189890
2
894


TM.orf2882
metC
cystathionine beta-lyase
3191071
3189935
−3
1137


TM.orf2883

Biotin synthase 1
3192783
3191149
−1
1635


TM.orf2884

conserved hypothetical protein
3194217
3193033
−1
1185


TM.orf2885
mutL
DNA mismatch repair protein MutL
3196145
3194217
−2
1929


TM.orf2886
pgi
Glucose-6-phosphate isomerase
3197443
3196160
−3
1284


TM.orf2887

protease
3198930
3197473
−1
1458


TM.orf2888

peptidase, M16 family
3200453
3198927
−2
1527


TM.orf2889

conserved hypothetical protein
3201124
3200570
−3
555


TM.orf2890
lspA
signal peptidase II
3201750
3201214
−1
537


TM.orf2891
ileS
isoleucyl-tRNA synthetase
3204559
3201743
−3
2817


TM.orf2892
ribF
FAD synthase
3205664
3204687
−2
978


TM.orf2893

MaoC-like dehydratase
3206193
3205744
−1
450


TM.orf2894

epoxide hydrolase
3207378
3206482
−1
897


TM.orf2895

hypothetical protein
3207569
3207859
3
291


TM.orf2896
ybjE
conserved hypothetical protein
3207856
3208455
1
600


TM.orf2897

Alcohol dehydrogenase zinc-binding domain
3209452
3208460
−3
993




protein


TM.orf2898
ydeS
TetR family transcriptional regulator
3210229
3209510
−3
720


TM.orf2899
emrB
major facilitator superfamily permease
3211827
3210226
−1
1602


TM.orf2900
emrA
secretion protein HlyD
3212940
3211843
−1
1098


TM.orf2901

hypothetical protein
3213113
3212973
−2
141


TM.orf2902
sigK
RNA polymerase sigma factor
3214108
3214683
1
576


TM.orf2903

conserved hypothetical protein
3214680
3215468
2
789


TM.orf2904

extracellular solute-binding protein
3215562
3216545
2
984


TM.orf2905

iron(III) transport system permease protein
3216650
3218272
3
1623


TM.orf2906
ugpC
ABC transporter component
3218269
3219384
1
1116


TM.orf2907

FAD dependent oxidoreductase
3220579
3219395
−3
1185


TM.orf2908

TetR family transcriptional regulator
3220742
3221344
3
603


TM.orf2909
map
methionine aminopeptidase, type I, Map
3221341
3222165
1
825




putative


TM.orf2910

conserved hypothetical protein
3222268
3223383
1
1116


TM.orf2911
tsr
putative methyl-accepting chemotaxis protein
3223543
3225231
1
1689


TM.orf2912

putative methyl-accepting chemotaxis protein
3225458
3227146
3
1689


TM.orf2913

conserved hypothetical protein
3227181
3227645
2
465


TM.orf2914

conserved hypothetical protein
3228498
3227650
−1
849


TM.orf2915
eutB
ethanolamine ammonia-lyase heavy chain
3228683
3230098
3
1416


TM.orf2916
eutC
Ethanolamine ammonia-lyase
3230095
3230928
1
834


TM.orf2917
ycaQ
conserved hypothetical protein
3232153
3230915
−3
1239


TM.orf2918

putative lactonase precursor
3232435
3233472
1
1038


TM.orf2919
rimJ
GCN5-related N-acetyltransferase
3233568
3234098
2
531


TM.orf2920
hmuV
ABC transporter related protein
3235006
3234191
−3
816


TM.orf2921

transport system permease protein
3236055
3235006
−1
1050


TM.orf2922

periplasmic binding protein
3237179
3236052
−2
1128


TM.orf2923

conserved hypothetical protein
3237469
3237176
−3
294


TM.orf2924
cirA
TonB-dependent receptor
3239539
3237536
−3
2004


TM.orf2925
nahR
Transcriptional regulator, LysR family
3240606
3239626
−1
981


TM.orf2926
degU
response regulator
3240884
3241528
3
645


TM.orf2927
yxjM
putative signal transduction histidine kinase
3241525
3243357
1
1833


TM.orf2928

truncated FmtB
3243493
3244641
1
1149


TM.orf2929

hypothetical protein
3244953
3246122
2
1170


TM.orf2930

conserved hypothetical protein
3246119
3247663
3
1545


TM.orf2931
adhA
alcohol dehydrogenase
3248702
3247671
−2
1032


TM.orf2932

hypothetical protein
3248839
3248681
−3
159


TM.orf2933
acoR
Fis family GAF modulated sigma54 specific
3249372
3251333
2
1962




transcriptional regulator


TM.orf2934

Cobalt transport protein ATP-binding subunit
3252222
3251398
−1
825


TM.orf2935

cobalt transport integral membrane protein
3252524
3252222
−2
303


TM.orf2936

cobalt ABC transporter, inner membrane
3252979
3252521
−3
459




subunit CbiQ


TM.orf2937
cbiN
cobalt transport protein
3253308
3252979
−1
330


TM.orf2938
cbiM
cobalamin biosynthesis protein cbim
3253979
3253305
−2
675


TM.orf2939

conserved hypothetical protein
3254522
3254896
3
375


TM.orf2940

putative RNA polymerase, sigma-24 subunit,
3254893
3256143
1
1251




ECF subfamily


TM.orf2941

hypothetical protein
3256528
3256127
−3
402


TM.orf2942
slmA
transcriptional regulatory protein
3256620
3257273
2
654


TM.orf2943

Oxidoreductase FAD/NAD(P)-binding
3258577
3257249
−3
1329


TM.orf2944
slyA
MarR family transcriptional regulator
3259153
3258638
−3
516


TM.orf2945

membrane protein of unknown function
3260016
3259150
−1
867


TM.orf2946
yecE
conserved hypothetical protein
3261081
3261875
2
795


TM.orf2947
yhaZ
conserved hypothetical protein
3262984
3261872
−3
1113


TM.orf2948

conserved hypothetical protein
3263683
3263087
−3
597


TM.orf2949
hutU
urocanate hydratase
3265396
3263723
−3
1674


TM.orf2950
hutH
phenylalanine/histidine ammonia-lyase
3267021
3265471
−1
1551


TM.orf2951

putative glycine betaine/L-proline transport
3267847
3267026
−3
822




ATP binding subunit


TM.orf2952
proW
histidine transport system permease ABC
3268697
3267840
−2
858




transporter protein


TM.orf2953
proX
Glycine betaine-binding periplasmic protein
3269858
3268815
−2
1044


TM.orf2954
hutC
GntR family transcriptional regulator
3270101
3270859
3
759


TM.orf2955
mdeA
methionine gamma-lyase
3272131
3270926
−3
1206


TM.orf2956
lrp
transcriptional regulator, AsnC family
3272249
3272707
3
459


TM.orf2957

transglutaminase domain protein
3272807
3273718
3
912


TM.orf2958

2-hydroxy-3-oxopropionate reductase
3273870
3274793
2
924


TM.orf2959
ybfI
transcriptional regulator, AraC family
3275542
3274745
−3
798


TM.orf2960

conserved hypothetical protein
3275668
3276291
1
624


TM.orf2961

plasmid stabilization system
3276601
3276302
−3
300


TM.orf2962

addiction module antidote protein, CC2985
3276854
3276591
−2
264




family


TM.orf2963
yddQ
putative hydrolase
3277476
3276931
−1
546


TM.orf2964
glxA
AraC family transcriptional regulator
3277598
3278584
3
987


TM.orf2965
rplU
50S ribosomal protein L21P
3279122
3279430
3
309


TM.orf2966
rpmA
50S ribosomal protein L27
3279545
3279805
3
261


TM.orf2967
obg
GTP-binding protein Obg/CgtA
3280029
3281519
2
1491


TM.orf2968
proB
gamma-glutamyl kinase
3281516
3282721
3
1206


TM.orf2969
proA
gamma-glutamyl phosphate reductase
3282718
3284061
1
1344


TM.orf2970
nadD
nicotinate (nicotinamide) nucleotide
3284126
3284737
3
612




adenylyltransferase


TM.orf2971
ybeB
lojap-related protein
3284865
3285269
2
405


TM.orf2972
rlmH
Ribosomal RNA large subunit
3285397
3285870
1
474




methyltransferase H


TM.orf2973

conserved hypothetical protein
3285905
3286588
3
684


TM.orf2974
gpmI
phosphoglycerate mutase
3286725
3288329
2
1605


TM.orf2975
yibP
Peptidase M23B
3288334
3289740
1
1407


TM.orf2976
ctpA
carboxyl-terminal processing protease
3289834
3291231
1
1398


TM.orf2977

conserved hypothetical protein
3291269
3292579
3
1311


TM.orf2978
rppH
nudix family hydrolase
3292662
3293138
2
477


TM.orf2979
modA
molybdate ABC transporter, periplasmic
3293246
3294043
3
798




molybdate-binding protein


TM.orf2980
modB
molybdate ABC transporter, permease protein
3294078
3294839
2
762


TM.orf2981
modC
molybdate transporter ATP-binding protein
3294836
3295957
3
1122


TM.orf2982
mopB
putative molybdenum-binding transcriptional
3296390
3295932
−2
459




regulator ModE family protein


TM.orf2983

conserved hypothetical protein
3297205
3296390
−3
816


TM.orf2984
atpC
F-type H+-transporting ATPase epsilon chain
3297891
3297490
−1
402


TM.orf2985
atpD
F1 -ATP Synthase Beta Chain
3299373
3297949
−1
1425


TM.orf2986
atpG
F-type H+-transporting ATPase gamma chain
3300342
3299449
−1
894


TM.orf2987
atpA
primosome assembly protein PriA
3302050
3300515
−3
1536


TM.orf2988
atpH
F0F1 ATP synthase subunit delta
3302607
3302047
−1
561


TM.orf2989

primosome assembly protein PriA
3305120
3302916
−2
2205


TM.orf2990
tal
transaldolase
3305466
3306122
2
657


TM.orf2991

conserved hypothetical protein
3306180
3306977
2
798


TM.orf2992
xerC
Tyrosine recombinase xerC
3306962
3307984
3
1023


TM.orf2993
pcoA
multicopper oxidase
3309420
3307975
−1
1446


TM.orf2994

hypothetical protein
3309811
3309482
−3
330


TM.orf2995
crp
transcriptional regulator, Crp
3310559
3309834
−2
726


TM.orf2996

hypothetical protein
3312135
3310825
−1
1311


TM.orf2997
lolD
ABC transporter, ATP-binding protein
3312949
3312218
−3
732


TM.orf2998

efflux ABC transporter, permease protein
3314076
3312949
−1
1128


TM.orf2999

auxiliary transport protein, MFP family
3315350
3314073
−2
1278


TM.orf3000

hypothetical protein
3315558
3315881
2
324


TM.orf3001
rpoE
RNA polymerase sigma-70 factor, ECF
3315994
3316557
1
564




subfamily


TM.orf3002

conserved hypothetical protein
3316554
3317003
2
450


TM.orf3003

conserved hypothetical protein
3317000
3317461
3
462


TM.orf3004
LPD1
dihydrolipoamide dehydrogenase
3318981
3317569
−1
1413


TM.orf3005
sucB
2-oxoglutarate dehydrogenase, E2
3320307
3319057
−1
1251




component, dihydrolipoamide




succinyltransferase


TM.orf3006
sucA
2-oxoglutarate dehydrogenase E1 component
3323323
3320432
−3
2892


TM.orf3007
sucD
Succinyl-CoA synthetase alpha chain
3324264
3323389
−1
876


TM.orf3008
sucC
succinyl-CoA synthetase, beta subunit
3325436
3324267
−2
1170


TM.orf3009
mdh
malate dehydrogenase
3326560
3325616
−3
945


TM.orf3010

AFG1 family ATPase
3328140
3326962
−1
1179


TM.orf3011
ywrD
gamma-glutamyltranspeptidase
3328425
3329972
2
1548


TM.orf3012
asd
aspartate-semialdehyde dehydrogenase
3330149
3331141
3
993


TM.orf3013
paiB
Protease synthase and sporulation protein PAI
3331307
3331975
3
669


TM.orf3014

hypothetical protein
3332533
3331976
−3
558


TM.orf3015
dppF
DppF
3333765
3332776
−1
990


TM.orf3016
dppD
dipeptide ABC superfamily ATP binding
3334762
3333758
−3
1005




cassette transporter, ABC protein


TM.orf3017
dppC
peptide/nickel transport system permease
3335658
3334762
−1
897




protein


TM.orf3018
dppB
nickel-transporting ATPase
3336750
3335740
−1
1011


TM.orf3019
dppA
dipeptide-binding ABC transporter substrate-
3338498
3336903
−2
1596




binding component


TM.orf3020
yqjG
putative glutathione S-transferase
3339743
3338727
−2
1017


TM.orf3021

Flavoprotein WrbA
3340444
3339830
−3
615


TM.orf3022

transcriptional regulator
3340592
3341506
3
915


TM.orf3023

conserved hypothetical protein
3341543
3341980
3
438


TM.orf3024

putative hydrolase
3342081
3342911
2
831


TM.orf3025
yerD
glutamate synthase
3344576
3342924
−2
1653


TM.orf3026
dapB
Dihydrodipicolinate reductase, bacterial
3345433
3344573
−3
861


TM.orf3027

two-component hybrid sensor and regulator
3347128
3345491
−3
1638


TM.orf3028
gitA
citrate synthase I
3348662
3347349
−2
1314


TM.orf3029

conserved hypothetical protein
3349785
3348889
−1
897


TM.orf3030

cytochrome c, class I
3350426
3349782
−2
645


TM.orf3031

hypothetical protein
3351351
3350485
−1
867


TM.orf3032

phenylacetic acid degradation-like protein
3351529
3351951
1
423


TM.orf3033

histidine triad protein
3352065
3352511
2
447


TM.orf3034
cya
adenylate/guanylate cyclase
3352516
3353817
1
1302


TM.orf3035

ribosomal large subunit pseudouridine
3354697
3353828
−3
870




synthase D


TM.orf3036

endoribonuclease L-PSP
3354836
3355204
3
369


TM.orf3037
nudI
putative NUDIX hydrolase
3355213
3355674
1
462


TM.orf3038
CMO
dioxygenase
3356860
3355649
−3
1212


TM.orf3039

putative lactone-dependent transcriptional
3357527
3356826
−2
702




regulator (tetr-family), mmfr


TM.orf3040
ytcB
NAD-dependent epimerase/dehydratase
3357911
3358969
3
1059


TM.orf3041

dTDP-4-dehydrorhamnose 3,5-epimerase
3358977
3359543
2
567


TM.orf3042

NAD dependent epimerase/dehydratase family
3359540
3360484
3
945


TM.orf3043

oxidoreductase
3360565
3361692
1
1128


TM.orf3044
crt
enoyl-CoA hydratase/isomerase
3361740
3362543
2
804


TM.orf3045

conserved hypothetical protein
3362634
3363560
2
927


TM.orf3046

LmbE-like protein
3364894
3364127
−3
768


TM.orf3047

Acyl-CoA dehydrogenase
3366072
3364888
−1
1185


TM.orf3048

glycosyl transferase family 2
3367241
3366069
−2
1173


TM.orf3049
PIP
proline iminopeptidase
3368566
3367547
−3
1020


TM.orf3050
yfgC
peptidase M48 Ste24p
3368909
3370357
3
1449


TM.orf3051

thioesterase superfamily protein
3370850
3370371
−2
480


TM.orf3052

thioesterase superfamily protein
3371302
3370868
−3
435


TM.orf3053
rpoH
RNA polymerase sigma-32 factor
3372489
3371590
−1
900


TM.orf3054
ydeB
Transcriptional regulators, similar to
3373223
3372726
−2
498




M. xanthus CarD


TM.orf3055
fdxA
4Fe—4S ferredoxin iron-sulfur binding domain-
3374015
3373677
−2
339




containing protein


TM.orf3056
hslR
heat shock protein
3374698
3374264
−3
435


TM.orf3057

Helicase-like protein
3377337
3374695
−1
2643


TM.orf3058
htpG
TPG_MAGMM RecName: Full = Chaperone
3377583
3379529
2
1947




protein htpG; AltName: Full = Heat shock




protein htpG; AltName: Full = High temperature




protein G


TM.orf3059
ycaD
major facilitator transporter
3381061
3379517
−3
1545


TM.orf3060
acnA
aconitate hydratase
3381716
3381291
−2
426


TM.orf3061
acn
aconitate hydratase
3383994
3381724
−1
2271


TM.orf3062
braF
high-affinity branched-chain amino acid
3384871
3385668
1
798




transport ATP-binding protein


TM.orf3063

Long-chain acyl-CoA synthetases (AMP-
3385665
3387596
2
1932




forming)


TM.orf3064
braD
inner-membrane translocator
3387696
3388589
2
894


TM.orf3065
IivM
branched-chain amino acid transport system
3388609
3389670
1
1062




permease protein


TM.orf3066

branched-chain amino acid transport system
3389828
3391060
3
1233




substrate-binding protein


TM.orf3067
IivF
ABC transporter related protein
3391149
3392006
2
858


TM.orf3068
paaK
phenylacetate--CoA ligase
3392075
3393316
3
1242


TM.orf3069
hpsA
3-hexulose-6-phosphate synthase
3394166
3393423
−2
744


TM.orf3070

dihydroxy-acid dehydratase
3395893
3394163
−3
1731


TM.orf3071

hypothetical protein
3396063
3395899
−1
165


TM.orf3072

trap dicarboxylate transporter- dctm subunit
3397390
3396074
−3
1317


TM.orf3073

TRAP-type mannitol/chloroaromatic compound
3397901
3397380
−2
522




transport system, small permease component


TM.orf3074
yiiZ
TRAP dicarboxylate family transporter, DctP
3398998
3397976
−3
1023




subunit


TM.orf3075

transcriptional regulatory protein
3399123
3399842
2
720


TM.orf3076

hypothetical protein
3400517
3399870
−2
648


TM.orf3077
ccmA
heme ABC exporter, ATP-binding protein
3400672
3401334
1
663




CcmA


TM.orf3078
helB
heme exporter protein CcmB
3401331
3402005
2
675


TM.orf3079
cycZ
heme exporter protein CcmC
3402323
3402895
3
573


TM.orf3080

hypothetical protein
3403067
3403330
3
264


TM.orf3081
ccmE
CcmE/CycJ protein
3403314
3403874
2
561


TM.orf3082
cycK
cytochrome c biogenesis factor
3403874
3405889
3
2016


TM.orf3083
helX
cytochrome c biogenesis protein
3405902
3406438
3
537


TM.orf3084
ccmH
protein involved in biosynthesis of c-type
3406435
3407139
1
705




cytochromes


TM.orf3085
cycH
cytochrome c-type biogenesis protein CycH
3407136
3408560
2
1425


TM.orf3086
aotP
Arginine/ornithine transport ATP-binding
3408690
3409466
2
777




protein aotP


TM.orf3087
petP
MarR family transcriptional regulator
3409604
3410125
3
522


TM.orf3088
occT
extracellular solute-binding protein family 3
3410322
3411095
2
774


TM.orf3089
hisQ
polar amino acid ABC transporter, inner
3411236
3411958
3
723




membrane subunit


TM.orf3090
occM
polar amino acid transport system permease
3411962
3412648
3
687




protein


TM.orf3091
yisB
conserved hypothetical protein
3412717
3412950
1
234


TM.orf3092
IivH
branched-chain amino acid ABC transporter
3413234
3414094
3
861




(permease)


TM.orf3093
braE
inner-membrane translocator
3414099
3415142
2
1044


TM.orf3094
braF
branched-chain amino acid ABC transporter
3415139
3415915
3
777




ATP-binding protein


TM.orf3095
IivF
branched-chain amino acid ABC transporter
3415902
3416609
2
708




ATP-binding protein


TM.orf3096

extracellular ligand-binding receptor
3416695
3417903
1
1209


TM.orf3097
queF
GTP cyclohydrolase family protein
3418466
3417993
−2
474


TM.orf3098

conserved hypothetical protein
3418835
3419149
3
315


TM.orf3099

conserved hypothetical protein
3419154
3419504
2
351


TM.orf3100
folD
FolD bifunctional protein
3419570
3420466
3
897


TM.orf3101

conserved hypothetical protein
3420498
3421088
2
591


TM.orf3102

conserved hypothetical protein
3421639
3421070
−3
570


TM.orf3103

conserved hypothetical protein
3421938
3421645
−1
294


TM.orf3104
hss
homospermidine synthase
3423511
3422096
−3
1416


TM.orf3105
argG
argininosuccinate synthase
3424922
3423708
−2
1215


TM.orf3106

Nephrocystin-3
3427879
3425117
−3
2763


TM.orf3107
cyaA
adenylate cyclase, family 3
3429289
3427979
−3
1311


TM.orf3108

Glutathione S-transferase domain protein
3429468
3430160
2
693


TM.orf3109

beta-lactamase domain-containing protein
3430166
3431068
3
903


TM.orf3110
pqqE
pyrroloquinoline quinone biosynthesis protein
3432137
3431073
−2
1065




PqqE


TM.orf3111

coenzyme PQQ synthesis D
3432424
3432134
−3
291


TM.orf3112
pqqC
pyrroloquinoline quinone biosynthesis protein
3433185
3432421
−1
765




PqqC


TM.orf3113
pqqB
coenzyme PQQ biosynthesis protein B
3434081
3433182
−2
900


TM.orf3114

hypothetical protein
3434225
3434100
−2
126


TM.orf3115
rlmN
cfr family radical SAM enzyme
3435617
3434352
−2
1266


TM.orf3116

conserved hypothetical protein
3436238
3435684
−2
555


TM.orf3117

conserved hypothetical protein
3436513
3437232
1
720


TM.orf3118
cya2
adenylyl cyclase class-3/4/guanylyl cyclase
3437330
3438481
3
1152


TM.orf3119

conserved hypothetical protein
3439105
3440133
1
1029


TM.orf3120
purH
bifunctional
3440241
3441824
2
1584




phosphoribosylaminoimidazolecarboxamide




formyltransferase/IMP cyclohydrolase


TM.orf3121

hypothetical protein
3441914
3442384
3
471


TM.orf3122

conserved hypothetical protein
3442872
3442390
−1
483


TM.orf3123

conserved hypothetical protein
3443297
3442869
−2
429


TM.orf3124

NAD-glutamate dehydrogenase
3448211
3443370
−2
4842


TM.orf3125

conserved hypothetical protein
3449196
3448420
−1
777


TM.orf3126
mhbM
3-hydroxybenzoate 6-hydroxylase
3450939
3449662
−1
1278


TM.orf3127

MarR family transcriptional regulator
3451172
3451669
3
498


TM.orf3128

conserved hypothetical protein
3451809
3452450
2
642


TM.orf3129

TRAP dicarboxylate transporter- DctM subunit
3452447
3453766
3
1320


TM.orf3130

conserved hypothetical protein
3453790
3454284
1
495


TM.orf3131
siaP
TRAP dicarboxylate transporter- DctP subunit
3454360
3455430
1
1071


TM.orf3132

Ala-tRNA(Pro) hydrolase
3455535
3456035
2
501


TM.orf3133
trxA
thioredoxin
3456220
3457137
1
918


TM.orf3134
IonD
Peptidase S16, Ion-like protein
3457253
3457921
3
669


TM.orf3135

conserved hypothetical protein
3457941
3458153
2
213


TM.orf3136

conserved hypothetical protein
3458158
3458556
1
399


TM.orf3137

Ubiquinone biosynthesis hydroxylase,
3460270
3459056
−3
1215




UbiH/UbiF/VisC/COQ6


TM.orf3138
glnB
nitrogen regulatory protein P-II
3460624
3460962
1
339


TM.orf3139

ammonium transporter
3460989
3462314
2
1326


TM.orf3140
aatC
aminotransferase class I and II
3462541
3463752
1
1212


TM.orf3141
ftsK
DNA segregation ATPase FtsK/SpoIIIE
3463826
3466243
3
2418


TM.orf3142
lolA
Outer membrane lipoprotein carrier protein
3466269
3466949
2
681




LolA


TM.orf3143

Na+-driven multidrug efflux pump
3466974
3468320
2
1347


TM.orf3144
puuD
putative glutamine amidotransferase
3468377
3469162
3
786


TM.orf3145

conserved hypothetical protein
3469186
3469800
1
615


TM.orf3146
alsT
putative amino acid carrier transmembrane
3469958
3471373
3
1416




protein


TM.orf3147

intracellular PHB depolymerase
3472706
3471480
−2
1227


TM.orf3148
sco1
Classical-complement-pathway C3/C5
3473694
3473074
−1
621




convertase


TM.orf3149
cobD
Cobalamin biosynthesis protein
3473990
3475003
3
1014


TM.orf3150

SH3 domain-containing YSC84-like protein
3475748
3475056
−2
693


TM.orf3151
cobP
Adenosylcobinamide-phosphate
3476453
3475914
−2
540




guanylyltransferase


TM.orf3152

Phosphoglycerate mutase
3477076
3476447
−3
630


TM.orf3153

hypothetical protein
3477915
3477067
−1
849


TM.orf3154
cobT
nicotinate-nucleotide--dimethylbenzimidazole
3478146
3479183
2
1038


TM.orf3155
yddV
diguanylate cyclase
3479436
3480347
2
912


TM.orf3156
sigK
RNA polymerase sigma-70 factor, ECF family
3480622
3481158
1
537




protein


TM.orf3157
chrR
Anti-sigma factor ChrR
3481158
3481880
2
723


TM.orf3158

conserved hypothetical protein
3481960
3482967
1
1008


TM.orf3159
lpsJ
3-oxoadipate CoA-transferase, beta subunit
3483693
3483052
−1
642


TM.orf3160
lpsI
3-oxoadipate CoA-transferase subunit A
3484379
3483696
−2
684


TM.orf3161
sam
RNA polymerase sigma-54 factor
3484716
3485678
2
963


TM.orf3162

hypothetical protein
3485675
3486301
3
627


TM.orf3163

3-hydroxyacyl-CoA dehydrogenase type-2
3486519
3487283
2
765


TM.orf3164

pterin-4-alpha-carbinolamine dehydratase
3487679
3487371
−2
309


TM.orf3165

conserved hypothetical protein
3487806
3488243
2
438


TM.orf3166
ybgC
Acyl-CoA thioester hydrolase ybgC
3488700
3488275
−1
426


TM.orf3167

conserved hypothetical protein
3489298
3489618
1
321


TM.orf3168
gltX
glutamyl-tRNA synthetase, class Ic
3490728
3489820
−1
909


TM.orf3169
mhpE
pyruvate carboxyltransferase
3491016
3492002
2
987


TM.orf3170

PqqC-like protein
3492074
3492838
3
765


TM.orf3171
ilvE
branched-chain amino acid aminotransferase-
3492931
3493884
1
954




like protein


TM.orf3172

conserved hypothetical protein
3493934
3495322
3
1389


TM.orf3173
dlpA
Dimethylmenaquinone methyltransferase
3495385
3496095
1
711


TM.orf3174
divJ
putative non-motile and phage-resistance
3496222
3497631
1
1410




protein


TM.orf3175

HNH endonuclease family protein
3497817
3498515
2
699


TM.orf3176
aspC
possible aspartate transaminase
3498661
3499797
1
1137


TM.orf3177
thiE
thiamine-phosphate pyrophosphorylase
3500432
3499794
−2
639


TM.orf3178
thiM
Hydroxyethylthiazole kinase
3501238
3500429
−3
810


TM.orf3179
coq7
ubiquinone biosynthesis protein COQ7
3502079
3501501
−2
579


TM.orf3180

disulfide bond formation protein, DsbB family
3502650
3502105
−1
546


TM.orf3181

TRAP transporter solute receptor TAXI family
3502874
3504154
3
1281




protein


TM.orf3182
pepB
Leucyl aminopeptidase
3504275
3505681
3
1407


TM.orf3183

conserved hypothetical protein
3505814
3507070
3
1257


TM.orf3184
ada
transcriptional regulator, AraC family
3508283
3507045
−2
1239


TM.orf3185
yejF
putative ATP-binding component of ABC
3510038
3508401
−2
1638




transporter


TM.orf3186
yejE
binding-protein-dependent transport systems
3511088
3510180
−2
909




inner membrane component


TM.orf3187
yejB
peptide/nickel transport system permease
3512177
3511092
−2
1086




protein


TM.orf3188

ABC-type oligopeptide transport system,
3514064
3512196
−2
1869




periplasmic component


TM.orf3189

Inositol monophosphatase
3514926
3514123
−1
804


TM.orf3190
cycM
putative CytoChrome c-like protein
3515700
3515083
−1
618


TM.orf3191
kdsB
3-deoxy-manno-octulosonate
3516147
3516881
2
735




cytidylyltransferase


TM.orf3192
ADT1
prephenate dehydratase
3516989
3517876
3
888


TM.orf3193
qacE
small multidrug resistance protein
3517958
3518305
3
348


TM.orf3194
ydcN
transcriptional regulator, XRE family with cupin
3519017
3518373
−2
645




sensor


TM.orf3195
ilvE
branched-chain amino acid aminotransferase
3520135
3519266
−3
870


TM.orf3196
ybbP
ABC transporter permease ybbP
3523075
3520439
−3
2637


TM.orf3197
ybbA
ABC transporter related protein
3523761
3523072
−1
690


TM.orf3198
tesA
SGNH hydrolase-type arylesterase
3524000
3524653
3
654


TM.orf3199

carbon monoxide dehydrogenase subunit G
3524776
3525519
1
744


TM.orf3200

transferase hexapeptide repeat containing
3526095
3525565
−1
531




protein


TM.orf3201

L-carnitine dehydratase/bile acid-inducible
3527392
3526133
−3
1260




protein F


TM.orf3202
yngG
pyruvate carboxyltransferase
3528387
3527440
−1
948


TM.orf3203
benM
transcriptional regulator, LysR family
3529436
3528513
−2
924


TM.orf3204

conserved hypothetical protein
3529858
3529433
−3
426


TM.orf3205
BphI
lactonase
3530062
3530904
1
843


TM.orf3206

conserved hypothetical protein
3531622
3530864
−3
759


TM.orf3207

conserved hypothetical protein
3531763
3532068
1
306


TM.orf3208

conserved hypothetical protein
3532080
3532586
2
507


TM.orf3209
yadG
ABC-type multidrug transport system, ATPase
3534029
3533049
−2
981




component


TM.orf3210
lbsA
Lactobacillus shifted protein
3534259
3534438
1
180


TM.orf3211
polA
DNA polymerase I
3534664
3537480
1
2817


TM.orf3212

HhH-GPD family protein
3537477
3538469
2
993


TM.orf3213

conserved hypothetical protein
3538466
3538942
3
477


TM.orf3214

conserved hypothetical protein
3538974
3540389
2
1416


TM.orf3215

hypothetical protein
3540683
3540396
−2
288


TM.orf3216

conserved hypothetical protein
3541151
3540885
−2
267


TM.orf3217
hspH
heat shock protein, Hsp20 family
3541701
3541243
−1
459


TM.orf3218

Transcription initiation factor TFIIIB, Brf1
3542719
3542366
−3
354




subunit/Transcription initiation factor TFIIB


TM.orf3219
aroA
3-phosphoshikimate 1-carboxyvinyltransferase
3543011
3544378
3
1368


TM.orf3220
cmk
cytidylate kinase
3544403
3545047
3
645


TM.orf3221
rpsA
Ribosomal protein S1
3545443
3547167
1
1725


TM.orf3222

HD domain protein
3547383
3547958
2
576


TM.orf3223

sulfatase
3547999
3549504
1
1506


TM.orf3224

Abortive infection bacteriophage resistance
3550482
3549514
−1
969




protein Abi


TM.orf3225
cbbR
putative transcriptional regulator
3551469
3550579
−1
891


TM.orf3226
ugpB
sn-glycerol-3-phosphate-binding periplasmic
3551620
3552942
1
1323




protein


TM.orf3227
ugpA
sn-glycerol-3-phosphate ABC transporter
3553017
3553922
2
906




permease


TM.orf3228
ugpE
sn-glycerol-3-phosphate transport system
3553909
3554838
1
930




permease protein


TM.orf3229
sppA
signal peptide peptidase SppA, 36K type
3554947
3555903
1
957


TM.orf3230
ihfB
Bacterial nucleoid DNA-binding protein
3556054
3556335
1
282


TM.orf3231

hypothetical protein
3556477
3556851
1
375


TM.orf3232
pyrF
orotidine-5′-phosphate decarboxylase
3557039
3557800
3
762


TM.orf3233
trpF
N-(5′phosphoribosyl)anthranilate isomerase
3557804
3558508
3
705


TM.orf3234
trpB
tryptophan synthase subunit beta
3558529
3559743
1
1215


TM.orf3235
trpA
RPA_AZOBR RecName: Full = Tryptophan
3559740
3560612
2
873




synthase alpha chain gb


TM.orf3236
accD
acetyl-CoA carboxylase carboxyl transferase
3560668
3561687
1
1020




subunit beta


TM.orf3237
FOL3
folC bifunctional protein
3561746
3563083
3
1338


TM.orf3238
ytxM
carboxyl esterase
3563117
3564007
3
891


TM.orf3239
cyaA
putative adenylate/guanylate cyclase
3566353
3564008
−3
2346


TM.orf3240
FPGS
FolC bifunctional protein
3567653
3566331
−2
1323


TM.orf3241
trxA
thioredoxin 1
3568048
3567725
−3
324


TM.orf3242
addA
UvrD/REP helicase
3571704
3568213
−1
3492


TM.orf3243

conserved hypothetical protein
3574796
3571701
−2
3096


TM.orf3244

nucleotidyl transferase
3575568
3574801
−1
768


TM.orf3245

aminoglycoside phosphotransferase
3576644
3575565
−2
1080


TM.orf3246

ATP-binding protein RP013
3577213
3576641
−3
573


TM.orf3247
ffh
signal recognition particle, subunit SRP54
3577543
3578898
1
1356


TM.orf3248
rpsP
30S ribosomal protein S16
3578997
3579362
2
366


TM.orf3249
rimM
50S ribosomal protein L19
3579412
3579993
1
582


TM.orf3250
trmD
tRNA (guanine-N1)-methyltransferase
3579990
3580757
2
768


TM.orf3251
rplS
50S ribosomal protein L19
3580972
3581352
1
381


TM.orf3252
leuC
3-isopropylmalate/(R)-2-methylmalate
3581677
3583095
1
1419




dehydratase large subunit


TM.orf3253
leuD
3-isopropylmalate dehydratase small subunit
3583134
3583751
2
618


TM.orf3254
leuB
Isocitrate/isopropylmalate dehydrogenase
3583878
3584987
2
1110


TM.orf3255
asd
Aspartate-semialdehyde dehydrogenase,
3585223
3586281
1
1059




USG-1


TM.orf3256

oxidoreductase domain protein
3586295
3586954
3
660


TM.orf3257
fabG
putative 3-oxoacyl-
3587684
3586980
−2
705


TM.orf3258

trap dicarboxylate transporter- dctp subunit
3587917
3589041
1
1125


TM.orf3259

TRAP-T family transporter, DctQ (4 TMs)
3589069
3589629
1
561




subunit


TM.orf3260
siaT
trap-t family transporter, dctm (12 tms) subunit
3589629
3590936
2
1308


TM.orf3261

transcriptional regulator, TetR family protein
3591010
3591693
1
684


TM.orf3262

enoyl-CoA hydratase
3591720
3592520
2
801


TM.orf3263

CoA-binding protein
3592517
3593758
3
1242


TM.orf3264

GNAT family acetyltransferase
3593875
3594702
1
828


TM.orf3265
yngJ
putative acyl-CoA dehydrogenase yngJ
3594699
3595880
2
1182


TM.orf3266
yecA
amino acid transporter
3598008
3596713
−1
1296


TM.orf3267

Cupin domain protein
3598343
3598005
−2
339


TM.orf3268
ycgG
conserved hypothetical protein
3599129
3598347
−2
783


TM.orf3269
lysM
transcription regulator
3599342
3599839
3
498


TM.orf3270
pdxY
pyridoxamine kinase
3600724
3599858
−3
867


TM.orf3271
citE
citrate lyase beta subunit
3601172
3602044
3
873


TM.orf3272

Acyl dehydratase
3602055
3603122
2
1068


TM.orf3273
sdhC
succinate dehydrogenase cytochrome b-556
3603352
3603738
1
387




subunit


TM.orf3274
sdhD
succinate dehydrogenase, hydrophobic
3603788
3604168
3
381




membrane anchor protein


TM.orf3275
sdhA
succinate dehydrogenase flavoprotein subunit
3604176
3605966
2
1791


TM.orf3276
sdhB
succinate dehydrogenase iron-sulfur subunit
3606010
3606783
1
774


TM.orf3277
ygaD
ABC transporter ATP-binding/permease
3608760
3607006
−1
1755


TM.orf3278

ABC transporter related protein
3610532
3608757
−2
1776


TM.orf3279

major facilitator transporter
3611773
3610529
−3
1245


TM.orf3280
fyuA
TonB-dependent receptor
3613947
3611788
−1
2160


TM.orf3281
pchR
helix-turn-helix domain-containing protein
3614138
3615079
3
942


TM.orf3282

hypothetical protein
3614145
3613993
−1
153


TM.orf3283

urease-associated protein
3615596
3615351
−2
246


TM.orf3284
yegD
chaperone protein yegD
3616092
3617390
2
1299


TM.orf3285

alanine racemase domain-containing protein
3618219
3617413
−1
807


TM.orf3286

HPr kinase
3619309
3618341
−3
969


TM.orf3287

conserved hypothetical protein
3619452
3620324
2
873


TM.orf3288

WbuT
3621042
3620362
−1
681


TM.orf3289

hypothetical protein
3621383
3621243
−2
141


TM.orf3290

glycosyl transferase family 2
3622369
3621461
−3
909


TM.orf3291

conserved hypothetical protein
3622716
3622423
−1
294


TM.orf3292

conserved hypothetical protein
3623628
3622744
−1
885


TM.orf3293

extradiol ring-cleavage dioxygenase III subunit B
3624532
3623735
−3
798


TM.orf3294

DoxD-like family protein
3625025
3624582
−2
444


TM.orf3295
nahR
transcriptional regulator MexT
3625214
3626170
3
957


TM.orf3296

isomerase
3626969
3626145
−2
825


TM.orf3297

short-chain dehydrogenase/reductase SDR
3627802
3626993
−3
810


TM.orf3298

CoxK
3628846
3627869
−3
978


TM.orf3299
pecT
HTH-type transcriptional regulator
3629067
3630047
2
981


TM.orf3300
kefC
potassium efflux system protein
3630112
3631839
1
1728


TM.orf3301
icfA
Carbonate dehydratase
3632556
3631864
−1
693


TM.orf3302
ugpQ
glycerophosphoryl diester phosphodiesterase
3632751
3633533
2
783


TM.orf3303
ugpB
extracellular solute-binding protein, family 1
3633629
3634957
3
1329


TM.orf3304
ugpA
SN-glycerol-3-phosphate ABC transporter,
3635054
3635941
3
888




permease protein


TM.orf3305
ugpE
sn-glycerol-3-phosphate ABC transporter,
3635957
3636811
3
855




permease protein


TM.orf3306
ugpC
glycerol-3-phosphate transporter ATP-binding
3637061
3638218
3
1158




subunit


TM.orf3307
mscS
MscS mechanosensitive ion channel
3639138
3638236
−1
903


TM.orf3308

TM2
3639549
3639205
−1
345


TM.orf3309

2-nitropropane dioxygenase NPD
3640112
3639657
−2
456


TM.orf3310

putative nitropropane dioxygenase
3640765
3640112
−3
654


TM.orf3311
gitR
regulatory protein, LysR: LysR, substrate-
3640840
3641739
1
900




binding


TM.orf3312

phospholipase/carboxylesterase
3642450
3641746
−1
705


TM.orf3313

phospholipase/carboxylesterase
3643159
3642488
−3
672


TM.orf3314

F-box protein SKIP8
3643373
3643801
3
429


TM.orf3315

multiple antibiotic resistance (MarC)-related
3643901
3644626
3
726




protein


TM.orf3316
ppx
Exopolyphosphatase
3646169
3644631
−2
1539


TM.orf3317
ppk
Polyphosphate kinase
3648626
3646239
−2
2388


TM.orf3318

putative phosphohistidine phosphatase, SixA
3648772
3649290
1
519


TM.orf3319

antibiotic biosynthesis monooxygenase
3649628
3649284
−2
345


TM.orf3320

Glycine hydroxymethyltransferase
3650926
3649625
−3
1302


TM.orf3321
argD
acetylornithine and succinylornithine
3652146
3650962
−1
1185


TM.orf3322
benE
benzoate transporter
3653378
3652143
−2
1236


TM.orf3323
mocR
GntR family transcriptional regulator
3653578
3655107
1
1530


TM.orf3324
Gde1
glycerophosphodiester phosphodiesterase
3656230
3655403
−3
828


TM.orf3325
yfiQ
CoA-binding domain protein
3658988
3656235
−2
2754


TM.orf3326

HAD-superfamily subfamily IIA hydrolase,
3659981
3659109
−2
873




hypothetical 3: HAD-superfamily hydrolase,




subfamily IIA


TM.orf3327

diguanylate phosphodiesterase
3661334
3660045
−2
1290


TM.orf3328
pyrD
Dihydroorotate dehydrogenase
3662385
3661303
−1
1083


TM.orf3329

conserved hypothetical protein
3662783
3662382
−2
402


TM.orf3330
argE
acetylornithine deacetylase (ArgE)
3664125
3662839
−1
1287


TM.orf3331
trkH
trk system potassium uptake protein TrkH
3665726
3664275
−2
1452


TM.orf3332
folE
GTP cyclohydrolase I
3666626
3665961
−2
666


TM.orf3333
apaG
ApaG
3667368
3666961
−1
408


TM.orf3334
metZ
O-succinylhomoserine sulfhydrylase
3668810
3667530
−2
1281


TM.orf3335
ynjD
ABC transporter related protein
3669666
3669016
−1
651


TM.orf3336
ynjC
inner membrane ABC transporter permease
3671393
3669663
−2
1731




protein YnjC


TM.orf3337
ynjB
ABC transporter, periplasmic binding protein
3672705
3671401
−1
1305


TM.orf3338

hypothetical protein
3673748
3672804
−2
945


TM.orf3339
traG
TRAG family protein
3675901
3673769
−3
2133


TM.orf3340

hypothetical protein
3676961
3676122
−2
840


TM.orf3341
icd
isocitrate dehydrogenase
3678798
3677308
−1
1491


TM.orf3342
yhdG
putative transmembrane permease protein
3679162
3680625
1
1464


TM.orf3343
maoI
tyramine oxidase
3680702
3682624
3
1923


TM.orf3344

hypothetical protein
3682673
3683380
3
708


TM.orf3345
virS
AraC family transcriptional regulator
3683558
3684568
3
1011


TM.orf3346
xthA
exonuclease III
3684652
3685458
1
807


TM.orf3347
gsiB
extracellular solute-binding protein, family 5
3686923
3685520
−3
1404


TM.orf3348
hslU
ATP-dependent protease ATP-binding subunit
3688537
3687224
−3
1314




HslU


TM.orf3349
hslV
ATP-dependent protease peptidase subunit
3689097
3688537
−1
561


TM.orf3350

conserved hypothetical protein
3690866
3689211
−2
1656


TM.orf3351
ycaC
isochorismatase hydrolase
3691676
3690963
−2
714


TM.orf3352
cpo
putative haloperoxidase protein
3692564
3691734
−2
831


TM.orf3353
HPA3
GCN5-related N-acetyltransferase
3693508
3693056
−3
453


TM.orf3354
yijE
inner membrane transporter yiJE
3694436
3693513
−2
924


TM.orf3355

acetyltransferase protein
3695155
3694433
−3
723


TM.orf3356
mocR
aminotransferase protein
3695271
3696674
2
1404


TM.orf3357
dgdR
LysR family transcriptional regulator
3697594
3696671
−3
924


TM.orf3358
hisB
imidazoleglycerol-phosphate dehydratase
3697747
3698352
1
606


TM.orf3359

hypothetical protein
3698393
3698851
3
459


TM.orf3360
hisH
imidazole glycerol phosphate synthase subunit
3698993
3699637
3
645




hisH


TM.orf3361
hisA
phosphoribosylformimino-5-aminoimidazole
3699708
3700439
2
732




carboxamide ribotide


TM.orf3362
hisF
imidazole glycerol phosphate synthase subunit
3700494
3701261
2
768




HisF


TM.orf3363
hisI
phosphoribosyl-AMP
3701377
3701835
1
459




cyclohydrolase/phosphoribosyl-ATP




pyrophosphohydrolase


TM.orf3364
hisE
phosphoribosyl-ATP pyrophosphohydrolase
3701816
3702190
3
375


TM.orf3365

conserved hypothetical protein
3702304
3702672
1
369


TM.orf3366

Membrane flavodoxin oxidoreductase
3702745
3704115
1
1371


TM.orf3367
dgoK
2-dehydro-3-deoxygalactonokinase
3704189
3705103
3
915


TM.orf3368
dgoA
2-dehydro-3-deoxy-6-phosphogalactonate
3705161
3705793
3
633




aldolase


TM.orf3369

conserved hypothetical protein
3706414
3705794
−3
621


TM.orf3370

conserved hypothetical protein
3707291
3706407
−2
885


TM.orf3371
mauG
methylamine utilization protein
3708680
3707562
−2
1119


TM.orf3372

conserved hypothetical protein
3709534
3708677
−3
858


TM.orf3373
hemR
iron complex outermembrane recepter protein
3711626
3709548
−2
2079


TM.orf3374

conserved hypothetical protein
3712214
3711762
−2
453


TM.orf3375

conserved hypothetical protein
3712816
3712292
−3
525


TM.orf3376

hypothetical protein
3713156
3712809
−2
348


TM.orf3377

transcriptional regulator, AraC family
3714267
3713281
−1
987


TM.orf3378
nepI
major facilitator superfamily MFS_1
3714406
3715620
1
1215


TM.orf3379

Sugar kinases, ribokinase family
3716470
3715598
−3
873


TM.orf3380

HTH-type transcriptional regulator
3716902
3716489
−3
414


TM.orf3381

Smr protein/MutS2
3717771
3716899
−1
873


TM.orf3382

conserved hypothetical protein
3718475
3717780
−2
696


TM.orf3383
lutB
Iron-sulfur cluster binding protein
3719953
3718472
−3
1482


TM.orf3384

putative Fe—S oxidoreductase
3720788
3719970
−2
819


TM.orf3385
mltA
membrane-bound lytic murein
3722192
3720909
−2
1284




transglycosylase A


TM.orf3386

cytochrome P450
3723432
3722260
−1
1173


TM.orf3387

prenyltransferase and squalene oxidase
3725420
3723429
−2
1992




repeat domain protein


TM.orf3388

HPr(Ser) kinase/phosphatase
3726331
3725417
−3
915


TM.orf3389
asnO
Asparagine synthase (glutamine-hydrolyzing)
3728229
3726328
−1
1902


TM.orf3390

conserved hypothetical protein
3728504
3728229
−2
276


TM.orf3391

hypothetical protein
3728685
3728542
−1
144


TM.orf3392
bioI
cytochrome P450 enzyme
3729760
3729026
−3
735


TM.orf3393

hypothetical protein
3730089
3729778
−1
312


TM.orf3394

conserved hypothetical protein
3730769
3730086
−2
684


TM.orf3395
ytzA
FxsA
3730977
3731501
2
525


TM.orf3396
secB
preprotein translocase subunit SecB
3731642
3732124
3
483


TM.orf3397
yhjC
LysR family transcriptional regulator
3733121
3732210
−2
912


TM.orf3398
padA
NmrA family protein
3733216
3734100
1
885


TM.orf3399

class D beta-lactamase
3734157
3735041
2
885


TM.orf3400
dnaQ
DNA polymerase III, epsilon subunit and
3735732
3735046
−1
687




related 3′-5′ exonuclease


TM.orf3401
coaE
Dephospho-CoA kinase
3736443
3735775
−1
669


TM.orf3402
aroE
shikimate 5-dehydrogenase
3737348
3736437
−2
912


TM.orf3403

Nucleotide-binding protein implicated in
3737986
3737348
−3
639




inhibition of septum formation


TM.orf3404

phosphotransferase
3738944
3737991
−2
954


TM.orf3405
hemE
uroporphyrinogen decarboxylase
3739697
3740749
3
1053


TM.orf3406
hemH
Protoheme ferro-lyase
3740758
3741849
1
1092


TM.orf3407

membrane protein
3741890
3742336
3
447


TM.orf3408
rho
transcription termination factor Rho
3743021
3744280
3
1260


TM.orf3409
qor
NADPH: quinone reductase and related Zn-
3745374
3744400
−1
975




dependent oxidoreductase


TM.orf3410

carboxymethylenebutenolidase
3746263
3745568
−3
696


TM.orf3411
ogt
methylated-DNA-
3746941
3746366
−3
576


TM.orf3412
yyaL
conserved hypothetical protein
3749004
3746938
−1
2067


TM.orf3413

conserved hypothetical protein
3749135
3749389
3
255


TM.orf3414
fdx4
ferredoxin, 2Fe—2S
3749397
3749807
2
411


TM.orf3415
mnmE
tRNA modification GTPase TrmE
3749964
3751349
2
1386


TM.orf3416
mnmG
tRNA uridine 5-carboxymethylaminomethyl
3751553
3753502
3
1950




modification enzyme GidA


TM.orf3417
rsmG
S-adenosylmethionine-dependent
3753508
3754236
1
729




methyltransferase involved in cell division


TM.orf3418
parA
chromosome segregation ATPase
3754208
3755032
3
825


TM.orf3419
parB
chromosome partitioning protein parB
3755029
3755961
1
933


TM.orf3420

DNA polymerase III, delta subunit
3757059
3756025
−1
1035


TM.orf3421

LPS-assembly lipoprotein
3757593
3757075
−1
519


TM.orf3422
leuS
leucyl-tRNA synthetase
3760225
3757640
−3
2586


TM.orf3423

conserved hypothetical protein
3760903
3760355
−3
549


TM.orf3424

Outer membrane protein (porin)
3761439
3762518
2
1080


TM.orf3425

Outer membrane protein (porin)
3762847
3763941
1
1095


TM.orf3426

Outer membrane protein (porin)
3764382
3765479
2
1098


TM.orf3427

sulfotransferase
3765670
3766569
1
900


TM.orf3428

conserved hypothetical protein
3766566
3767432
2
867


TM.orf3429

putative enzyme with PLP-binding domain
3768118
3768804
1
687




alanine racemase


TM.orf3430

conserved hypothetical protein
3769318
3768809
−3
510


TM.orf3431

conserved hypothetical protein
3770663
3769416
−2
1248


TM.orf3432
ribA
GTP cyclohydrolase II
3772029
3770914
−1
1116


TM.orf3433

YceI family protein
3772739
3772092
−2
648


TM.orf3434

CDP-alcohol phosphatidyltransferase
3773527
3772739
−3
789


TM.orf3435

deaminase-reductase domain-containing
3773762
3774832
3
1071




protein


TM.orf3436
kanE
FAD dependent oxidoreductase
3774829
3775785
1
957


TM.orf3437

conserved hypothetical protein
3775834
3776253
1
420


TM.orf3438

putative glycosyl transferase
3776268
3777335
2
1068


TM.orf3439

Methyltransferase type 12
3777335
3778162
3
828


TM.orf3440
sphR
two component transcriptional regulator
3778236
3778937
2
702


TM.orf3441
nahR
transcriptional regulator, LysR family
3779852
3778944
−2
909


TM.orf3442

ThiJ/PfpI domain protein
3779976
3780632
2
657


TM.orf3443

hypothetical protein
3779977
3779864
−3
114


TM.orf3444
fadB
enoyl-CoA hydratase/isomerase
3781441
3780680
−3
762


TM.orf3445

transcriptional regulators
3782264
3781557
−2
708


TM.orf3446
yifB
Predicted ATPase with chaperone activity
3783896
3782283
−2
1614


TM.orf3447

conserved hypothetical protein
3785042
3784608
−2
435


TM.orf3448
nth
endonuclease III
3785229
3785960
2
732


TM.orf3449

conserved hypothetical protein
3786067
3786216
1
150


TM.orf3450
ppa
inorganic pyrophosphatase
3787457
3786930
−2
528


TM.orf3451
ampG
major facilitator superfamily permease
3788915
3787551
−2
1365


TM.orf3452
typA
GTP-binding protein TypA/BipA
3790767
3788941
−1
1827


TM.orf3453

Sugar kinase
3791831
3790839
−3
993


TM.orf3454

conserved hypothetical protein
3792175
3792870
2
696


TM.orf3455

hypothetical protein
3792902
3793315
1
414


TM.orf3456

tetratricopeptide TPR_4
3794296
3793322
−1
975


TM.orf3457

type I phosphodiesterase/nucleotide
3795910
3794378
−1
1533




pyrophosphatase


TM.orf3458
yihL
putative transcriptional regulator
3796104
3796805
3
702


TM.orf3459
flhA
alcohol dehydrogenase class III
3796872
3797984
3
1113


TM.orf3460
Esd
intracellular septation protein A
3797989
3798858
2
870


TM.orf3461

hypothetical protein
3799428
3798883
−2
546


TM.orf3462
arsC
putative arsenate reductase
3799966
3799613
−1
354


TM.orf3463
gshB
glutathione synthetase
3800927
3799986
−3
942


TM.orf3464

tetratricopeptide repeat family protein
3801935
3801054
−3
882


TM.orf3465

Predicted periplasmic or secreted lipoprotein
3802603
3801932
−1
672


TM.orf3466

conserved hypothetical protein
3803051
3802647
−3
405


TM.orf3467

uroporphyrin-III C/tetrapyrrole
3804199
3803048
−1
1152




(corrin/porphyrin) methyltransferase


TM.orf3468

Extracellular ligand-binding receptor
3804283
3805578
2
1296


TM.orf3469

coproporphyrinogen III oxidase
3806806
3805607
−1
1200


TM.orf3470

Xanthosine triphosphate pyrophosphatase
3807426
3806815
−2
612


TM.orf3471
rph
RNase PH
3808418
3807441
−3
978


TM.orf3472
hrcA
Transcriptional regulator of heat shock gene
3808725
3809855
3
1131


TM.orf3473
grpE
GrpE protein
3810026
3810646
1
621


TM.orf3474
dnaK
molecular chaperone DnaK
3810999
3812927
3
1929


TM.orf3475
dnaJ
chaperone protein DnaJ
3813080
3814261
1
1182


TM.orf3476
dapB
dihydrodipicolinate reductase
3814452
3815207
3
756


TM.orf3477
yedA
inner membrane transporter yedA
3816161
3815238
−3
924


TM.orf3478
phbC
Poly-beta-hydroxybutyrate polymerase domain
3818234
3816252
−3
1983




protein


TM.orf3479

hypothetical protein
3818477
3818340
−3
138


TM.orf3480
yhiI
HlyD family secretion protein
3818514
3819494
3
981


TM.orf3481
yhiH
ABC transporter, ATP binding/permease
3819491
3822256
1
2766




protein


TM.orf3482
yhhJ
Inner membrane transport permease yhhJ
3822261
3823385
3
1125


TM.orf3483
hipA
HipA domain protein
3824820
3823498
−2
1323


TM.orf3484

hypothetical protein
3824978
3824820
−3
159


TM.orf3485
icc
putative ICC protein
3826129
3825308
−1
822


TM.orf3486
potA
putative ABC transporter
3827137
3826136
−1
1002


TM.orf3487
modB
binding-protein-dependent transport systems
3827937
3827134
−2
804




inner membrane component


TM.orf3488
cysW
putative permease protein
3828776
3827943
−3
834


TM.orf3489

family 1 extracellular solute-binding protein
3829893
3828871
−2
1023


TM.orf3490
purR
regulatory protein
3831030
3829978
−2
1053


TM.orf3491
mviN
virulence factor
3832713
3831157
−2
1557


TM.orf3492
glnD
[protein-PII] uridylyltransferase
3835601
3832800
−3
2802


TM.orf3493
metQ
YaeC family lipoprotein
3836668
3835802
−1
867


TM.orf3494
metQ
D-methionine ABC superfamily ATP binding
3837713
3836868
−3
846




cassette transporter, binding protein


TM.orf3495
mcp3
methyl-accepting chemotaxis sensory
3839287
3837932
−1
1356




transducer


TM.orf3496

esterase/lipase/thioesterase
3840277
3839309
−1
969


TM.orf3497

virulence protein
3841146
3840379
−2
768


TM.orf3498
mprF
putative membrane protein
3843692
3841143
−3
2550


TM.orf3499

branched chain amino acid ABC transporter
3845162
3843942
−3
1221




amino acid-binding protein


TM.orf3500
alkK
medium-chain-fatty-acid-CoA ligase
3845541
3847160
3
1620


TM.orf3501
mutS
DNA mismatch repair protein
3849952
3847139
−1
2814


TM.orf3502
tme
malic enzyme
3850401
3852698
3
2298


TM.orf3503
phoR
two-component sensor histidine kinase
3852914
3854356
1
1443


TM.orf3504
lcfB
acyl-CoA synthase
3854482
3856020
2
1539


TM.orf3505
sphX
phosphate ABC transporter, periplasmic
3856419
3857441
3
1023




binding protein


TM.orf3506
yqgH
phosphate ABC transporter, permease protein
3857697
3859079
3
1383




PstC


TM.orf3507
yqgI
phosphate ABC transporter, inner membrane
3859099
3860424
2
1326




subunit PstA


TM.orf3508

Phosphate import ATP-binding protein
3860520
3861290
3
771


TM.orf3509
phoU
Phosphate transport system protein PhoU
3861318
3862043
3
726


TM.orf3510
phoB
Response regulator
3862169
3862867
1
699


TM.orf3511
rhtB
lysine exporter protein LysE/YggA
3862963
3863589
2
627


TM.orf3512
trpS
Tryptophanyl-tRNA synthetase
3863762
3864754
1
993


TM.orf3513

putative permease
3864903
3865814
3
912


TM.orf3514

conserved hypothetical protein
3865814
3866596
1
783


TM.orf3515

universal stress family protein
3866612
3867124
1
513


TM.orf3516
cya1
adenylate cyclase
3868421
3867117
−3
1305


TM.orf3517
soxR
Redox-sensitive transcriptional activator soxR
3869016
3868549
−2
468


TM.orf3518

conserved hypothetical protein
3869119
3869538
2
420


TM.orf3519

RND efflux membrane fusion protein precursor
3869543
3870643
1
1101


TM.orf3520

RND efflux transporter
3870640
3873729
2
3090


TM.orf3521

thioredoxin
3873814
3874359
2
546


TM.orf3522

Diacylglycerol kinase, catalytic region
3876693
3875188
−2
1506




oxidoreductase, short chain


TM.orf3523
fabG
dehydrogenase/reductase family
3877381
3876680
−1
702


TM.orf3524
ddpX
D-alanyl-D-alanine dipeptidase
3877980
3877423
−2
558


TM.orf3525
yeaZ
peptidase M22, glycoprotease
3878054
3878785
1
732


TM.orf3526

ribosomal-protein-alanine acetyltransferase
3878782
3879258
2
477


TM.orf3527

transcriptional regulatory protein
3879811
3880233
2
423


TM.orf3528
fur
ferric uptake regulator family protein
3880551
3880997
3
447


TM.orf3529

hemolysin
3881199
3881978
3
780


TM.orf3530
plsC
1-acyl-sn-glycerol-3-phosphate
3881975
3882907
1
933




acyltransferase


TM.orf3531
miaB
RNA modification protein
3883012
3884415
2
1404


TM.orf3532
ybeZ
phosphate starvation-inducible protein PhoH
3884457
3885599
3
1143




and related proteins


TM.orf3533

Predicted metal-dependent hydrolase
3885596
3886255
1
660


TM.orf3534

CBS domain-containing protein
3886567
3887403
2
837


TM.orf3535
lnt
Apolipoprotein N-acyltransferase
3887442
3889046
3
1605


TM.orf3536

transcriptional regulator
3889352
3889789
1
438


TM.orf3537
metK
S-adenosylmethionine synthetase
3890091
3891260
3
1170


TM.orf3538
trmB
tRNA (guanine-N7-)-methyltransferase
3891360
3892103
3
744


TM.orf3539
rimP
Ribosome maturation factor rimP
3892428
3892922
3
495


TM.orf3540
nusA
transcription elongation factor NusA
3892931
3894490
1
1560


TM.orf3541

conserved hypothetical protein
3894501
3895187
3
687


TM.orf3542
infB
translation initiation factor IF-2
3895198
3898329
2
3132


TM.orf3543
rbfA
ribosome-binding factor A
3898431
3898880
3
450


TM.orf3544
truB
Pseudouridine synthase
3898885
3899802
2
918


TM.orf3545
rpsO
ribosomal protein S15
3899820
3900089
3
270


TM.orf3546
pnp
polyribonucleotide nucleotidyltransferase
3900584
3902830
1
2247


TM.orf3547

Dioxygenases related to 2-nitropropane
3903207
3904556
3
1350




dioxygenase


TM.orf3548
fur
Fe2+/Zn2+ uptake regulation proteins
3904706
3905116
1
411


TM.orf3549
rbr
Rubrerythrin
3905178
3905681
3
504


TM.orf3550

conserved hypothetical protein
3906332
3905733
−3
600


TM.orf3551
gyaR
lactate dehydrogenase and related
3906637
3907617
2
981




dehydrogenase


TM.orf3552

conserved hypothetical protein
3907757
3908482
1
726


TM.orf3553

conserved hypothetical protein
3908952
3908497
−2
456


TM.orf3554

molybdopterin biosynthesis protein
3909780
3908965
−2
816


TM.orf3555
cysK
cysteine synthase A
3910819
3909845
−1
975


TM.orf3556
iscR
transcriptional regulator
3911327
3910872
−3
456


TM.orf3557
dut
deoxyuridine 5′triphosphate
3911860
3911402
−1
459




nucleotidohydrolase protein


TM.orf3558

phosphopantothenoylcysteine
3913134
3911860
−2
1275




decarboxylase/phosphopantothenate--cysteine




ligase


TM.orf3559
ubiB
protein kinase
3914820
3913306
−2
1515


TM.orf3560
ubiE
2-octaprenyl-6-methoxy-1,4-benzoquinone
3915654
3914848
−2
807




methylase/demethylmenaquinone




methyltransferase


TM.orf3561
mutM
formamidopyrimidine-DNA glycosylase
3915740
3916603
1
864


TM.orf3562

conserved hypothetical protein
3916600
3917028
2
429


TM.orf3563

enoyl-CoA hydratase/isomerase family protein
3917177
3917950
1
774


TM.orf3564
rpsT
Ribosomal protein S20p
3918135
3918404
3
270


TM.orf3565

rhodanese domain-containing protein
3918958
3919428
2
471









Example 4
Conjugation of Tistrella mobilis Plasmid
Conjugation refers to the transfer of a plasmid from one bacterial cell to another. During conjugation, the bacterial cell that harbors the plasmid is regarded as the donor cell and the other cell lacking the plasmid is regarded as recipient cells. The donor cell must have a set of genes that are capable of doing two functions: 1) production of the sex pilus in which two bacterial cells are brought together and through which the plasmid DNA is transferred to the recipient cell, and 2) the DNA-processing component that processes the plasmid at a particular site so that a single strand of the plasmid is unwound and transferred to the recipient cell. The sex pilus in most Gram-negative bacteria is encoded by a set of 11 genes with conserved sequences and functions. Tistrella mobilis, being Gram-negative, is found to harbor the same set of genes on its chromosomal DNA. The corresponding 11 homologous genes can be found from orf TM.2187-2197, which in certain embodiments indicates the capability of T. mobilis to produce the sex pilus required for the conjugation process. Furthermore, plasmid 3, which harbors the didemnin gene cluster, is found to encode several genes that in some embodiments are involved in the DNA processing of the plasmid. Among them, P3.orf0026, encodes a relaxase, a central component for DNA processing, which further implies that plasmid 3 may be transferred to another cell by conjugation. Other nearby sequences of the relaxase also encode some other genes that are involved in the conjugation. For example, P3.orf0056 encodes a lytic transglycosylase, and it is believed to degrade bacterial cell wall for DNA transfer during conjugation.
Therefore, in specific embodiments plasmid 3 in which the didemnin gene cluster resides may transfer to another bacterial species. Other species in the genus of Tistrella could be candidates in embodiments wherein they do not have the same plasmid. Species from closely related genera, such as members of Azospirillum and Agrobacteria (for example only), may be recipients, in certain embodiments.
REFERENCES
All patents and publications mentioned in the specification are indicative of the level of those skilled in the art to which the invention pertains. All patents and publications are herein incorporated by reference in their entirety to the same extent as if each individual publication was specifically and individually indicated to be incorporated by reference.
Patents


    U.S. Pat. No. 5,294,603
    U.S. Pat. No. 5,399,363
    U.S. Pat. No. 5,466,468
    U.S. Pat. No. 5,543,158
    U.S. Pat. No. 5,580,579
    U.S. Pat. No. 5,629,001
    U.S. Pat. No. 5,641,515
    U.S. Pat. No. 5,792,451
    U.S. Pat. No. 6,613,308
    U.S. Pat. No. 6,841,530


PUBLICATIONS


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    Fischbach M A, Walsh C T (2006) Assembly-line enzymology for polyketide and nonribosomal peptide antibiotics: Logic, machinery, and mechanisms. Chem Rev 106:3468-3496.
    Imker H J, Krahn D, Clerc J, Kaiser M, Walsh C T (2010) N-Acylation during Glidobactin Biosynthesis by the Tridomain Nonribosomal Peptide Synthetase Module GlbF. Chem Biol 17: 1077-1083
    Jou G, González I, Albericio F, Lloyd-Williams P, Giralt E. 1997. Total Synthesis of Dehydrodidemnin B. Use of Uronium and Phosphonium Salt Coupling Reagents in Peptide Synthesis in Solution. J Org Chem. 62:354-366.
    Lautru S, Oves-Costales D, Pernodet J L, Challis G L. (2007) MbtH-like protein-mediated cross-talk between non-ribosomal peptide antibiotic and siderophore biosynthetic pathways in Streptomyces coelicolor M145. Microbiology 153:1405-1412.
    Le Tourneau C, Faivre S, Ciruelos E, Domínguez M J, López-Martín J A, Izquierdo M A, Jimeno J, Raymond E. 2010. Reports of clinical benefit of plitidepsin (Aplidine), a new marine-derived anticancer agent, in patients with advanced medullary thyroid carcinoma. Am J Clin Oncol. 33:132-6.
    Marahiel M A, Stachelhaus T, Mootz H D. 1997. Modular peptide synthetases involved in nonribosomal peptide synthesis. Chemical reviews 97(7): 2651-2673
    Mateos M V, Cibeira M T, Richardson P G, Prosper F, Oriol A, de la Rubia J, Lahuerta J J, García-Sanz R, Extremera S, Szyldergemajn S, Corrado C, Singer H, Mitsiades C S, Anderson K C, Bladé J, San Miguel J. 2010. Phase II clinical and pharmacokinetic study of plitidepsin 3-hour infusion every two weeks alone or with dexamethasone in relapsed and refractory multiple myeloma. Clin Cancer Res. 16:3260-3269.
    Mayer, S. C., Ramanjulu, J., Vers, M. D., Pfizenmayer, A. J., and Joullie, M. M., Synthesis of new didemnin B analogs for investigations of structure/biological activity relationships, J. Org. Chem., 1994, 59, 5192-5205.
    Ramaswamy A V, Sorrels C M, Gerwick W H. 2007. Cloning and biochemical characterization of the hectochlorin biosynthetic gene cluster from the marine cyanobacterium Lyngbya majuscule. J Nat. Prod. 70(12): 1977-1986
    Rausch C, Weber T, Kohlbacher O, Wohlleben W, Huson D H (2005) Specificity prediction of adenylation domains in nonribosomal peptide synthetases (NRPS) using Transductive Support Vector Machines (TSVM). Nucleic Acids Res 33: 5799-5808.
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    Shi, B.-H., et al., Tistrella mobilis gen. nov., sp. Nov., a novel polyhydroxyalkanoate-producing bacterium belonging to α-Proteobacteria, J. Gen. Appl. Microbiol., 48, 335-343 (2002).
    Soto-Matos A, Szyldergemajn S, Extremera S, Miguel-Lillo B, Alfaro V, Coronado C, Lardelli P, Roy E, Corrado C S, Kahatt C. 2011. Plitidepsin has a safe cardiac profile: a comprehensive analysis. Mar Drugs 9:1007-1023.
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Although the present invention and its advantages have been described in detail, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims. Moreover, the scope of the present application is not intended to be limited to the particular embodiments of the process, machine, manufacture, composition of matter, means, methods and steps described in the specification. As one of ordinary skill in the art will readily appreciate from the disclosure of the present invention, processes, machines, manufacture, compositions of matter, means, methods, or steps, presently existing or later to be developed that perform substantially the same function or achieve substantially the same result as the corresponding embodiments described herein may be utilized according to the present invention. Accordingly, the appended claims are intended to include within their scope such processes, machines, manufacture, compositions of matter, means, methods, or steps.

## Claims

1. A method of producing a didemnin precursor, didemnin, or didemnin derivative, comprising the steps of:
a) culturing a host cell harboring bacterial didemnin synthesis genes; and
b) recovering said didemnin precursor, didemnin, or didemnin derivative from said host cell,
wherein the host cell is an isolated Tistrella mobilis bacterium having Accession Deposit Number NRRL B-50531 with the depository Agricultural Research Service Culture Collection National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture.

2. A method of producing a didemnin precursor, didemnin, or didemnin derivative, comprising the steps of:
a) culturing a host cell harboring bacterial didemnin synthesis genes; and
b) recovering said didemnin precursor, didemnin, or didemnin derivative from said host cell,
wherein the host cell is:
(i) an isolated Tistrella mobilis bacterium having Accession Deposit Number NRRL B-50531 with the depository Agricultural Research Service Culture Collection National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture; or
(ii) a host cell comprising the polynucleotide sequences of SEQ ID NO:42-51.