EXHIBIT 31.1 CERTIFICATION PURSUANT TO RULE 13A-14(A) 
AS ADOPTED PURSUANT TO SECTION 302 OF
THE SARBANES-OXLEY ACT OF 2002 I, Kuslima Shogen, certify that:1.
I have reviewed this
 quarterly report on Form 10-Q of Alfacell Corporation;2.
Based on my knowledge,
 this report does not contain any untrue statement of a material fact or omit
 to state a material fact necessary to make the statements made, in light of
 the circumstances under which such statements were made, not misleading with
 respect to the period covered by this report;3.
Based on my knowledge,
 the financial statements, and other financial information included in this
 report, fairly present in all material respects the financial condition,
 results of operations and cash flows of the registrant as of, and for, the
 periods presented in this report;4.
The
 registrants other certifying officer and I are responsible for establishing
 and maintaining disclosure controls and procedures (as defined in Exchange
 Act Rules 13a-15(e) and 15d-15(e)) and internal control over financial
 reporting (as defined in Exchange Act Rules 13a-15(f) and 15d-15(f)) for the
 registrant and have:(a)
Designed
 such disclosure controls and procedures, or caused such disclosure controls
 and procedures to be designed under our supervision, to ensure that material
 information relating to the registrant is made known to us by others within
 those entities, particularly during the period in which this report is being
 prepared;(b)
Designed
 such internal control over financial reporting, or caused such internal
 control over financial reporting to be designed under our supervision to
 provide reasonable assurance regarding the reliability of financial reporting
 and the preparation of financial statements for external purposes in
 accordance with generally accepted accounting principles;(c)
Evaluated
 the effectiveness of the registrants disclosure controls and procedures and
 presented in this report our conclusions about the effectiveness of the
 disclosure controls and procedures, as of the end of the period covered by
 this report based on such evaluation; and(d)
Disclosed
 in this report any change in the registrants internal control over financial
 reporting that occurred during the registrants most recent fiscal quarter
 (the registrants fourth fiscal quarter in the case of an annual report) that
 has materially affected, or is reasonably likely to materially affect, the
 registrants internal control over financial reporting; and5.
The registrants other
 certifying officer and I have disclosed, based on our most recent evaluation
 of internal control over financial reporting, to the registrants auditors
 and the audit committee of the registrants board of directors (or persons
 performing the equivalent functions):(a)
All significant
 deficiencies and material weaknesses in the design or operation of internal
 control over financial reporting which are reasonably likely to adversely
 affect the registrants ability to record, process, summarize and report
 financial information; and(b)
Any fraud, whether or
 not material, that involves management or other employees who have a
 significant role in the registrants internal control over financial reporting. Date: December 10, 2008 /s/ Kuslima ShogenName: Kuslima ShogenTitle:   Chief
 Executive Officer(Principal
 Executive Officer)