# Errata

Validation of the

Larval Amphibian Growth and Development Assay:

Integrated Summary Report

U.S. Environmental Protection Agency

Endocrine Disruptor Screening Program

Washington, D.C.

June 21 , 2013

# Table of Contents

Forward

................................ ................................ ................................ ................................ ......................... 3

Prochloraz summary tables

................................ ................................ ................................ ........................... 3

Prochlor az appendix data tables

................................ ................................ ................................ .................... 6

4

- tert

- octylphenol summary tables

................................ ................................ ................................ ............. 10

4

- tert

- octylphenol appendix data tables

................................ ................................ ................................ ...... 11

17β

- trenbolone summary tables

................................ ................................ ................................ .................. 14

17 β

- trenbolone appendix data tables

................................ ................................ ................................ .......... 15

Benzophenone

- 2 summary tables

................................ ................................ ................................ ............... 17

Benzophenone

- 2 appendix data tables

................................ ................................ ................................ ........ 18

Control comparisons

................................ ................................ ................................ ................................ ... 20

# Forward

A portion of the inter

- laboratory data provide d in the LAGDA IS R was analyzed using the one

- way

ANOVA module of StatCHARRMS which recently was found to have an error in the code. In that

module the replicate data was not considered in the statistical analyses. Thus, the statistical unit of

analysis became the individual animal rather than the tank which resulted in erroneous estimates of the

following: biological variances, variances on effects due to treatment, and variances on estimates of

biological means. It also introduces the possibility of confounding treatment effects with possible tank

effects. The analyses in the other two modules of StatCHARRMS, a repeated measure ANOVA, and a

pathology analysis module (RSCABS), correctly incorporate the replicate structure of the test protocols. It

should be noted that the re

- coding of the one

- way ANOVA module in the open source ‘R’ version of

StatCHARRMS being developed by the Agency did not have this error and correctly identifies the

statistical sampling unit as the tank, when specified, and not the individual organism.

Specifically with respect to the LAGDA ISR , the ANOVA analyses performed on larval and juvenile

growth (length and weight), time to stage 62, and vitellogenin w ere run incorrectly. The individual was

used as the unit of replication rather than the tank. The se analys e s ha ve been correctly rerun and we

provi de in this document a set of corrected summary tables (found in the body of the ISR) and appendix

tables (found in the supporting appendix for the inter

- laborat ory evaluation portion of the I S R ) for

comparison to the original table (also provide in this do cument). In all cases statistical significan ce is

indicated on the table for easy comparison of the original analysis and the corrected analysis.

In some cases

, reports of statistical significance in the original LAGDA ISR are not accurate. However,

the impact of the reanalysis is not widespread

. I n general

, when the reanalysis was different from the

original, it tended to result in an increase in the lowest observed effective concentration (LOEC) reported

by one test con centration. In only two cases did the LOEC decrease. Consequently, this statistical error

does not impact the overall data interpr etation provided in the LAGDA I S R . We will provide a detailed

review of the situation during our presentation on Tuesday Ju ne 25 th



# .



# Prochloraz summary tables

The following summary tables are corrected to present the lowest observed effect levels based on the

corrected statistical analysis. They are presented in place of the tables found in section 5.3.1 of the

integrated summary report. Highlighted cells indicate a change in statistical outcome based on the

corrected analysis.

Summary of inter

- laboratory prochloraz growth endpoints.

All endpoints with lowest concentration statistically different than control. Concentrat ions listed as nominal

(180, 60, 20, 6.7 μg/L) regardless of mean measured concentrations. Yellow highlights indicate where the

LOEC increased following the reanalysis. Dash: Not significant in either analysis

. n. s

. : Not significant after

reanalysis

.

Lab A D B C

Larval sample

Weight

- 180 ug/L n

. s

. - Length

- 180 ug/L 180 ug/L n

. s

.

Juvenile Males

Weight

- 60 ug/L

- - Length

- 60 ug/L

- -

LSI 60 ug/L 6.7 ug/L 180 ug/L

-Juvenile Females

Weight

- 180 ug/L

- n

. s

. Length

- 180 ug/L n

. s

. -

LSI 60 ug/L 20 ug/L 20 ug/L n

. s

.



# Summary of inter

- laboratory prochloraz thyroid endpoints.

All endpoints with lowest concentration statistically different than control . Concentrations listed as nominal

(180, 60, 20, 6.7 μg/L) regardless of mean measured concentrations. Yellow highlights indicate where the

LOEC increased following the reanalysis. Dash: Not significant in either analysis

. n. s

. : Not significant after

reanalysis

.

Lab A D B C

Thyroid endpoints

Time to NF62

- n

. s.

- -

Serum T4 60 ug/L

- - - Thyroid pathology 180 ug/L 20 ug/L

- 6.7 ug/L



# Summary of inter

- laboratory prochloraz reproductive endpoints.

All endpoints with lowest concentration statistically different than control. Concentrations listed as nominal

(180, 60, 20, 6.7 μg/L) regardless of mean measured concentrations. Yellow highlights indicate where the

LOEC increased following the reanalysis

. Dash: Not significant in either analysis

. n.s. : Not significant after

reanalysis

.

Lab A D B C

Juvenile Males

Plasma VTG 60 ug/L n.s.

- 60 ug/L

Gonad pathology 6.7 ug/L 6.7 ug/L 6.7 ug/L 20 ug/L

Duct pathology 20 ug/L 60 ug/L 20 ug/L 20 ug/L

Juvenile Females

Plasma VTG 6.7 ug/L 180 ug/L

- 20 ug/L

Gonad pathology 180 ug/L 60 ug/L 20 ug/L 6.7 ug/L

Duct pathology 180 ug/L

- 180 ug/L

-

# Prochloraz appendix data tables

The following two tables represent the same original data. The first table is from Appendix 8.4.1 of the

integrated summary report. The second table represents the correct analysis of the original data. In this

case, there were no differences in the sta tistical outcome s

.

Table 8.4.1

- 2 (Original)

Larval growth and development (NF66) following prochloraz exposure at A (mean ± SD).

Nominal PZ concentration Weight (g) Length (mm) Time to NF66 (d)

0 ug/L 0.99 ± 0.16 21.0 ± 1.2 46 ± 2

6.7 ug/L 1.08 ± 0.22 21.7 ± 1.8 46 ± 3

20 ug/L 0.98 ± 0.11 21.0 ± 1.0 45 ± 2

60 ug/L 1.01 ± 0.21 20.8 ± 1.2 47 ± 3

180 ug/L 1.01 ± 0.14 20.8 ± 1.1 47 ± 5

Table 8.4.1

- 2 (Corrected)

Larval growth and development (NF66) following prochloraz exposure at A (mean ± SD).

Nominal PZ concentration Weight (g) Length (mm) Time to NF66 (d)

0 ug/L 0.99 ± 0.1 1 21 .0 ± 0.7 46 ± 1

6.7 ug/L 1.0 8 ± 0.09 21.7 ± 0.6 46 ± 2

20 ug/L 0.98 ± 0.0 3 21 .0 ± 0.3 45 ± 1

60 ug/L 1.0 1 ± 0.0 6 20.8 ± 0.1 47 ± 2

180 ug/L 1.0 1 ± 0.04 20.8 ± 0.4 48 ± 1

# The following two tables represent the same original data. The first table is from Appendix 8.4.1 of the

integrated summary report. The second table represents the correct analysis of the original data. In this

case there were differences in the statistical outcome

. Yellow shading ( ■ ) indicates statistical

significance with the previous incorrect analysis. Orange shading ( ■ ) in the second table indicates

statistical significance in the corrected analysis.

Table 8.4.1

- 3 (Original)

Interlaboratory comparison of larval growth and development (NF62) following prochloraz exposure (mean ± SD).

Highlighted cells are significantly different than control (α = 0.05).

Lab D B C

Nominal PZ concentration Weight (g)

0 ug/L 0.791 ± 0.130 1.08 ± 0.17 0.616 ± 0.139

6.7 ug/L 0.742 ± 0.244 1.21 ± 0.22 0.627 ± 0.104

20 ug/L 0.722 ± 0.204 1.08 ± 0.22 0.640 ± 0.124

60 ug/L 0.723 ± 0.153 1.13 ± 0.14 0.707 ± 0.154

180 ug/L 0.708 ± 0.168 0.92 ± 0.18 0.658 ± 0.174

Length (mm)

0 ug/L 18.8 ± 1.0 20.8 ± 1.0 17.1 ± 1.4

6.7 ug/L 18.5 ± 1.8 21.3 ± 1.2 17.5 ± 1.0

20 ug/L 18.4 ± 1.6 20.7 ± 1.2 16.9 ± 1.3

60 ug/L 18.3 ± 1.0 21.0 ± 0.9 18.1 ± 1.3

180 ug/L 18.1 ± 1.2 19.6 ± 1.2 16.8 ± 1.3

Time to NF62 (d)

0 ug/L 43 ± 2 41 ± 3 44 ± 6

6.7 ug/L 42 ± 2 39 ± 2 44 ± 4

20 ug/L 41 ± 2 40 ± 3 43 ± 5

60 ug/L 43 ± 2 41 ± 3 45 ± 5

180 ug/L 42 ± 2 40 ± 2 45 ± 10

Table 8.4.1

- 3 (Corrected)

Interlaboratory comparison of larval growth and development (NF62) following prochloraz exposure (mean ± SD).

Highlighted cells are significantly different than control (α = 0.05).

Lab D B C

Nominal PZ concentration Weight (g)

0 ug/L 0.792 ± 0.048 1.08 ± 0.11 0.616 ± 0.076

6.7 ug/L 0.742 ± 0.093 1.21 ± 0.11 0.627 ± 0.017

20 ug/L 0.722 ± 0.132 1.08 ± 0.20 0.64 0 ± 0.106

60 ug/L 0.724 ± 0.048 1.13 ± 0.0 7 0.707 ± 0.115

180 ug/L 0.708 ± 0.076 0.92 ± 0.07 0.658 ± 0.069

Length (mm)

0 ug/L 18.8 ± 0.3 20.8 ± 0.5 17.1 ± 0.7

6.7 ug/L 18.5 ± 0.5 21.3 ± 0.6 17.5 ± 0.1

20 ug/L 18.4 ± 1.1 20.7 ± 1.1 16.9 ± 1 .0

60 ug/L 18.3 ± 0.2 21 .0 ± 0.6 18.1 ± 1 .0

180 ug/L 18.1 ± 0.4 19.6 ± 0.5 16.8 ± 0.3

Time to NF62 (d)

0 ug/L 43 ± 1 4 1 ± 2 44 ± 2

6.7 ug/L 42 ± 1 39 ± 1 4 4 ± 1

20 ug/L 4 1 ± 1 40 ± 2 4 3 ± 3

60 ug/L 4 3 ± 1 41 ± 2 4 5 ± 3

180 ug/L 42 ± 2 40 ± 1 45 ± 6

# The following two tables represent the same original data. The first table is from Appendix 8.4.1 of the integrated summary report. The second

table represents the correct analysis of the original data. In this case there were differences in the statistical outcome. Yellow shading ( ■ )

indicates statistical significance with t he previous incorrect analysis. Orange shading ( ■ ) in the second table indicates statistical significance in the

corrected analysis.

Table 8.4.1

- 5 (Original)

Interlaboratory comparison of juvenile growth and plasma vitellogenin titers following prochloraz exposure (mean ± SD). Highlighted cells ar e significantly different than control (α = 0.05).

Male Female

A D B C A D B C

Nominal PZ concentration Weight (g) Weight (g)

0 ug/L 13.1 ± 2.0 18.6 ± 4.1 11.5 ± 3.6 4.0 ± 0.8 12.9 ± 2.6 20.0 ± 4.6 13.8 ± 5.0 4.1 ± 0.8

6.7 ug/L 12.5 ± 2.6 19.4 ± 3.8 13.2 ± 5.4 3.7 ± 0.5 13.8 ± 3.4 18.2 ± 3.0 12.3 ± 5.0 3.7 ± 0.6

20 ug/L 11.8 ± 2.8 17.6 ± 3.8 12.9 ± 5.0 4.0 ± 1.1 14.1 ± 2.3 20.3 ± 4.9 12.2 ± 4.8 4.2 ± 1.1

60 ug/L 12.6 ± 2.3 16.1 ± 3.9 12.5 ± 3.3 3.7 ± 0.8 13.8 ± 2.6 18.1 ± 4.4 12.0 ± 5.4 3.4 ± 0.7

180 ug/L 12.5 ± 2.7 10.8 ± 5.2 11.4 ± 4.3 4.0 ± 1.2 13.3 ± 2.8 14.3 ± 7.1 11.4 ± 4.5 4.7 ± 1.6

Length (mm) Length (mm)

0 ug/L 47.2 ± 2.8 53.0 ± 3.9 43.0 ± 4.5 32.8 ± 2.2 47.2 ± 2.6 55.0 ± 5.2 46.4 ± 5.4 32.9 ± 2.2

6.7 ug/L 46.4 ± 3.0 53.9 ± 4.1 44.8 ± 6.7 32.5 ± 1.3 47.9 ± 4.0 51.4 ± 8.6 43.8 ± 6.8 32.2 ± 1.9

20 ug/L 45.1 ± 4.2 52.0 ± 3.8 44.7 ± 5.4 31.9 ± 2.5 47.8 ± 2.0 55.2 ± 4.7 43.3 ± 5.4 33.5 ± 2.9

60 ug/L 46.9 ± 2.5 50.7 ± 4.0 44.1 ± 3.8 32.5 ± 2.4 47.7 ± 2.9 52.9 ± 4.5 43.0 ± 6.9 32.1 ± 1.8

180 ug/L 46.4 ± 3.8 43.6 ± 8.5 42.7 ± 6.2 32.2 ± 3.0 48.1 ± 3.7 48.3 ± 9.8 42.2 ± 6.8 33.9 ± 3.9

LSI LSI

0 ug/L 0.059 ± 0.010 0.058 ± 0.005 0.057 ± 0.009 0.035 ± 0.007 0.061 ± 0.007 0.059 ± 0.006 0.064 ± 0.014 0.032 ± 0.006

6.7 ug/L 0.056 ± 0.012 0.053 ± 0.012 0.064 ± 0.015 0.031 ± 0.005 0.056 ± 0.013 0.057 ± 0.006 0.059 ± 0.014 0.032 ± 0.007

20 ug/L 0.055 ± 0.012 0.054 ± 0.007 0.053 ± 0.014 0.034 ± 0.006 0.060 ± 0.007 0.055 ± 0.004 0.055 ± 0.010 0.032 ± 0.006

60 ug/L 0.052 ± 0.007 0.049 ± 0.005 0.052 ± 0.007 0.032 ± 0.007 0.051 ± 0.006 0.051 ± 0.006 0.056 ± 0.010 0.031 ± 0.005

180 ug/L 0.050 ± 0.004 0.040 ± 0.009 0.048 ± 0.009 0.037 ± 0.005 0.052 ± 0.005 0.044 ± 0.007 0.048 ± 0.011 0.037 ± 0.006

VTG (mg/mL) VTG (mg/mL)

0 ug/L 0.03 ± 0.02 0.01 ± 0.02 0.00 ± 0.00 0.00 ± 0.00 0.03 ± 0.03 0.02 ± 0.07 0.01 ± 0.02 0.03 ± 0.01

6.7 ug/L 0.05 ± 0.06 0.02 ± 0.04 0.01 ± 0.02 0.01 ± 0.002 0.07 ± 0.10 0.01 ± 0.01 0.03 ± 0.05 0.03 ± 0.02

20 ug/L 0.05 ± 0.04 0.01 ± 0.02 0.00 ± 0.00 0.01 ± 0.001 0.07 ± 0.10 0.01 ± 0.01 0.00 ± 0.00 0.04 ± 0.01

60 ug/L 0.12 ± 0.13 0.02 ± 0.03 0.00 ± 0.00 0.01 ± 0.004 0.12 ± 0.13 0.04 ± 0.07 0.00 ± 0.01 0.09 ± 0.05

180 ug/L 0.10 ± 0.10 0.02 ± 0.02 0.00 ± 0.01 0.01 ± 0.002 0.25 ± 0.32 0.04 ± 0.07 0.01 ± 0.01 0.10 ± 0.04Table 8.4.1

- 5 (Corrected)

Interlaboratory comparison of juvenile growth and plasma vitellogenin titers following prochloraz exposure (mean ± SD). Highlighted cells ar e significantly different than control (α = 0.05).

Male Female

A D B C A D B C

Nominal PZ concentration Weight (g) Weight (g)

0 ug/L 13.3 ± 1.1 18.3 ± 1.5 11.7 ± 1.3 4 .0 ± 0.2 12.9 ± 0.7 19.9 ± 2.6 13.7 ± 3.1 4.1 ± 0.5

6.7 ug/L 12.5 ± 1.4 19.4 ± 0.6 13.3 ± 1.3 3.7 ± 0.1 13.7 ± 1.1 18.2 ± 1.7 12.1 ± 1.5 3.7 ± 0.4

20 ug/L 11.8 ± 0.6 17.3 ± 1.8 12.7 ± 2.8 4.2 ± 1 .0 14.1 ± 0.6 20.5 ± 1.4 12.5 ± 3.9 4.2 ± 0.5

60 ug/L 12.5 ± 0.7 16.2 ± 0.8 12.9 ± 1.9 3.7 ± 0.5 13.9 ± 0.4 17.8 ± 2.1 11.8 ± 1.6 3.5 ± 0.2

180 ug/L 12.5 ± 0.8 11.6 ± 3.3 10.6 ± 2.4 4 .0 ± 0.2 13.3 ± 1.4 14.6 ± 2.8 11.8 ± 2.5 4.9 ± 0.9

Length (mm) Length (mm)

0 ug/L 47.4 ± 1.5 52.9 ± 1 .0 43. 3 ± 2.0 32.9 ± 0.7 47.2 ± 0.9 55 .0 ± 2.6 46.5 ± 2.8 32.8 ± 1.4

6.7 ug/L 46.4 ± 1.9 53.9 ± 0.8 4 5

. 0 ± 2.0 32.6 ± 0.4 47.9 ± 1.5 51.4 ± 3.7 43.6 ± 1.7 32.3 ± 1.1

20 ug/L 45.1 ± 0.9 51.7 ± 2 .0 44. 5 ± 2.6 32.4 ± 2.1 47.9 ± 0.8 55.4 ± 1.3 43.6 ± 4.6 33.4 ± 1.1

60 ug/L 46.7 ± 1 .0 50.7 ± 0.5 44.5 ± 2. 3 32.4 ± 1.8 47.9 ± 0.9 52.5 ± 2.4 42.8 ± 2 .0 32.2 ± 0.4

180 ug/L 46.4 ± 1.6 45 .0 ± 5.6 41.3 ± 3.4 32 .0 ± 0.5 48.1 ± 1.4 48.8 ± 4 .0 43 .0 ± 3.8 34.3 ± 1.6

LSI LSI

0 ug/L 0.059 ± 0.007 0.058 ± 0.002 0.057 ± 0.004 0.034 ± 0.005 0.061 ± 0.003 0.059 ± 0.003 0.067 ± 0.01 0 0.032 ± 0.004

6.7 ug/L 0.056 ± 0.005 0.053 ± 0.005 0.064 ± 0.005 0.031 ± 0.003 0.056 ± 0.009 0.057 ± 0.004 0.059 ± 0.005 0.032 ± 0.003

20 ug/L 0.055 ± 0.007 0.054 ± 0.003 0.052 ± 0.009 0.035 ± 0.004 0.06 0 ± 0.005 0.054 ± 0.001 0.056 ± 0.005 0.032 ± 0.001

60 ug/L 0.051 ± 0.004 0.049 ± 0.003 0.053 ± 0.004 0.033 ± 0.005 0.052 ± 0.002 0.051 ± 0.002 0.055 ± 0.002 0.031 ± 0.003

180 ug/L 0.05 0 ± 0.002 0.041 ± 0.003 0.047 ± 0.005 0.036 ± 0.004 0.052 ± 0.002 0.044 ± 0.004 0.049 ± 0.006 0.036 ± 0.003

VTG (mg/mL) VTG (mg/mL)

0 ug/L 0.033 ± 0.013 0.012 ± 0.01 0 0.002 ± 0.003 0.005 ± 0.002 0.033 ± 0.016 0.019 ± 0.032 0.006 ± 0.009 0.03 0 ± 0.006

6.7 ug/L 0.046 ± 0.031 0.022 ± 0.015 0.015 ± 0.018 0.006 ± 0.001 0.066 ± 0.05 0 0.013 ± 0.006 0.028 ± 0.018 0.032 ± 0.004

20 ug/L 0.047 ± 0.017 0.013 ± 0.009 0.001 ± 0.001 0.006 ± 0 .000 0.079 ± 0.063 0.009 ± 0.005 0.002 ± 0.003 0.039 ± 0.005

60 ug/L 0.113 ± 0.059 0.014 ± 0.013 0.002 ± 0.004 0.008 ± 0.001 0.131 ± 0.033 0.03 0 ± 0.028 0.002 ± 0.004 0.088 ± 0.023

180 ug/L 0.1 00 ± 0.053 0.027 ± 0.01 0 0.004 ± 0.006 0.007 ± 0.001 0.247 ± 0.172 0.036 ± 0.044 0.003 ± 0.004 0.1 00 ± 0.015

# 4

- tert

- octylphenol summary tables

The following summary tables are corrected to present the lowest observed effect levels based on the

corrected statistical analysis. They are presented in place of the tables found in section 5.3.2 of the

integrated summary report. Highlighted cells indicate a change in statistical outcome based on the

corrected analysis.

Summary of inter

- laboratory octylphenol growth endpoints.

All endpoints with lowest concentration statistically different than control.

Concentrations listed as nominal (50, 25, 12.5, 6.25 μg/L) regardless of mean measured

concentrations

. Yellow highlights indicate where the LOEC increased following the

reanalysis. Blue highlight indicates where the LOEC appeared following the reanalysis;

in this instance only there was a non

- monotonic effect noted (i.e. the only treatment

significantly different than control)

. Dash : Not significant in either analysis

. n.s. : Not

significant after reanalysis.

Lab A B C

Larval sample

Weight

- - - Length n.s. n.s.

-

Juvenile Males

Weight

- - - Length

- 25 μg/L*

-

LSI

- - 50 ug/L

Juvenile Females

Weight

- - - Length

- - 12.5 ug/L*

LSI

- - 25 ug/L

* Non

- monotonic (i.e. the only treatment significantly different than control)



# Summary of inter

- laboratory octylphenol thyroid endpoints.

All endpoints with lowest concentration statistically different than control.

Concentrations listed as nominal (50, 25, 12.5, 6.25 μg/L) regardless of mean measured

concentrations. Blue highlight indicate s where the LOEC decreased following the

reanalysi s. Dash: Not significant in either analysis

.

Lab A B C

Thyroid endpoints

Time to NF62 50 ug/L

- 50 μg/L

Serum T4 25 ug/L

- - Thyroid pathology 12.5 ug/L

- -



# Summary of inter

- laboratory octylphenol reproductive endpoints.

All endpoints with lowest concentration statistically different than control.

Concentrations listed as nominal (50, 25, 12.5, 6.25 μg/L) regardless of mean measured

concentrations. Yellow highlights indicate where the LOEC increased following the

reanaly sis. Dash: Not significant in either analysis

. n.s. : Not significant after reanalysis

.

Lab A B C

Juvenile Males

Plasma VTG 12.5 ug/L

- 6.25 ug/L

Gonad pathology

- 12.5 ug/L

- Duct pathology 6.25 ug/L 25 ug/L

-

Juvenile Females

Plasma VTG

- n.s. 12.5 ug/L

Gonad pathology 12.5 ug/L

- 12.5 ug/LDuct pathology 50 ug/L 12.5 ug/L 6.25 ug/L



# 4

- tert

- octylphenol appendix data tables

The following two tables represent the same original data. The first table is from Appendix 8.4.2 of the

integrated summary report. The second table represents the correct analysis of the original data. In this

case there were differences in the statistical outcome. Yellow shading ( ■ ) indicates statistical

significance with t he previous incorrect analysis. Orange shading ( ■ ) in the second table indicates

statistical significance in the corrected analysis.

Table 8.4.2

- 2 (Original)

Interlaboratory comparison of larval growth and development (NF62) following octylphenol

exposure (mean ± SD). Highlighted cells are significantly different than control (α = 0.05).

Lab A B C

Nominal OP concentration Weight (g)

0 ug/L 0.96 ± 0.15 2.07 ± 0.33 0.461 ± 0.14

6.25 ug/L 1.05 ± 0.14 2.04 ± 0.31 0.475 ± 0.15

12.5 ug/L 0.90 ± 0.15 2.17 ± 0.49 0.540 ± 0.13

25 ug/L 1.00 ± 0.16 2.11 ± 0.33 0.494 ± 0.13

50 ug/L 0.93 ± 0.19 2.19 ± 0.32 0.466 ± 0.09

Length (mm)

0 ug/L 19.2 ± 1.0 23.1 ± 1.3 15.2 ± 1.6

6.25 ug/L 19.9 ± 0.9 23.6 ± 1.1 15.3 ± 1.7

12.5 ug/L 19.0 ± 1.0 23.4 ± 1.6 16.0 ± 1.5

25 ug/L 19.8 ± 1.1 23.1 ± 1.4 15.5 ± 1.3

50 ug/L 18.9 ± 1.4 24.1 ± 1.4 15.5 ± 1.4

Time to NF62 (d)

0 ug/L 42 ± 5 42 ± 2 41 ± 10

6.25 ug/L 41 ± 3 43 ± 2 43 ± 13

12.5 ug/L 44 ± 5 44 ± 3 39 ± 10

25 ug/L 43 ± 4 43 ± 2 39 ± 8

50 ug/L 44 ± 5 43 ± 2 39 ± 9

Table 8.4.2

- 2 (Corrected)

Interlaboratory comparison of larval growth and development (NF62) following octylphenol

exposure (mean ± SD). Highlighted cells are significantly different than control (α = 0.05).

Lab A B C

Nominal OP concentration Weight (g)

0 ug/L 0.96 ± 0.08 2.07 ± 0.17 0.46 ± 0.03

6.25 ug/L 1.04 ± 0.11 2.04 ± 0.13 0.47 ± 0.06

12.5 ug/L 0.90 ± 0.09 2.17 ± 0.12 0.54 ± 0.08

25 ug/L 0.99 ± 0.12 2.11 ± 0.24 0.49 ± 0.08

50 ug/L 0.92 ± 0.16 2.19 ± 0.11 0.47 ± 0.04

Length (mm)

0 ug/L 19.2 ± 0.5 23.1 ± 0.5 15.2 ± 0.4

6.25 ug/L 19.9 ± 0.5 23.6 ± 0.5 15.3 ± 0.7

12.5 ug/L 19.0 ± 0.3 23.4 ± 0.8 16.0 ± 0.7

25 ug/L 19.7 ± 0.7 23.1 ± 0.8 15.5 ± 0.8

50 ug/L 18.9 ± 1.1 24.1 ± 0.9 15.5 ± 0.3

Time to NF62 (d)

0 ug/L 42 ± 1 42 ± 2 41 ± 3

6.25 ug/L 41 ± 0.4 43 ± 1 43 ± 4

12.5 ug/L 44 ± 1 44 ± 2 39 ± 2

25 ug/L 43 ± 2 43 ± 2 39 ± 1

50 ug/L 44 ± 2 43 ± 1 39 ± 3

# The following two tables represent the same original data. The first table is from Appendix 8.4. 2 of the

integrated summary report. The second table represents the correct analysis of the original data. In this

case there were differences in the statistical outcome. Yellow shading ( ■ ) indicates statistical

significance with the previous incorrect a nalysis. Orange shading ( ■ ) in the second table indicates

statistical significance in the corrected analysis.

Table 8.4.2

- 4 (Original)

Interlaboratory comparison of juvenile growth and plasma vitellogenin titers following octylphenol exposure (mean ± SD). Highlighted cells a re significantly different than control

(α = 0.05).

Male Female

A B C A B C

Nominal OP concentration Weight (g) Weight (g)

0 ug/L 10.9 ± 2.7 10.1 ± 2.6 8.6 ± 2.6 12.0 ± 2.7 10.9 ± 3.0 8.3 ± 1.7

6.25 ug/L 11.1 ± 2.0 10.0 ± 2.5 8.8 ± 2.8 12.4 ± 3.1 11.5 ± 3.1 9.1 ± 2.2

12.5 ug/L 11.5 ± 3.0 10.4 ± 2.4 8.0 ± 1.6 11.7 ± 2.9 10.3 ± 2.6 9.6 ± 2.7

25 ug/L 11.3 ± 3.0 10.8 ± 2.6 8.4 ± 2.0 12.1 ± 2.4 9.2 ± 4.0 7.5 ± 1.9

50 ug/L 10.9 ± 1.5 10.1 ± 3.2 7.8 ± 2.5 11.6 ± 3.0 11.6 ± 4.0 7.9 ± 2.0

Length (mm) Length (mm)

0 ug/L 43.1 ± 3.3 41.6 ± 3.5 41.1 ± 3.4 44.7 ± 4.0 42.5 ± 4.0 40.8 ± 3.1

6.25 ug/L 44.1 ± 2.8 40.7 ± 4.1 41.4 ± 3.9 46.1 ± 3.7 43.7 ± 4.3 41.9 ± 3.5

12.5 ug/L 43.1 ± 4.1 42.7 ± 3.5 41.8 ± 2.3 44.3 ± 4.0 43.5 ± 4.8 44.2 ± 4.0

25 ug/L 43.5 ± 3.6 43.8 ± 3.7 42.2 ± 3.2 44.5 ± 3.2 41.4 ± 5.9 40.8 ± 3.6

50 ug/L 43.3 ± 2.2 41.6 ± 4.5 41.2 ± 3.6 43.9 ± 4.3 44.5 ± 5.1 41.0 ± 3.4

LSI LSI

0 ug/L 0.058 ± 0.008 0.058 ± 0.007 0.072 ± 0.008 0.059 ± 0.012 0.059 ± 0.014 0.075 ± 0.007

6.25 ug/L 0.060 ± 0.010 0.056 ± 0.006 0.066 ± 0.009 0.059 ± 0.008 0.059 ± 0.005 0.073 ± 0.009

12.5 ug/L 0.058 ± 0.007 0.058 ± 0.008 0.070 ± 0.008 0.058 ± 0.004 0.062 ± 0.007 0.073 ± 0.007

25 ug/L 0.062 ± 0.010 0.062 ± 0.006 0.066 ± 0.009 0.061 ± 0.008 0.057 ± 0.012 0.069 ± 0.009

50 ug/L 0.066 ± 0.011 0.058 ± 0.007 0.063 ± 0.010 0.062 ± 0.013 0.057 ± 0.004 0.067 ± 0.006

VTG (mg/mL) VTG (mg/mL)

0 ug/L 0.000 ± 0.001 0.013 ± 0.014 0.005 ± 0.001 0.000 ± 0.001 0.008 ± 0.008 0.036 ± 0.011

6.25 ug/L 0.000 ± 0.001 0.009 ± 0.009 0.007 ± 0.003 0.000 ± 0.001 0.006 ± 0.007 0.042 ± 0.013

12.5 ug/L 0.001 ± 0.002 0.015 ± 0.034 0.018 ± 0.021 0.001 ± 0.001 0.010 ± 0.014 0.055 ± 0.009

25 ug/L 0.002 ± 0.004 0.007 ± 0.006 0.021 ± 0.016 0.001 ± 0.000 0.015 ± 0.017 0.097 ± 0.026

50 ug/L 0.001 ± 0.002 0.014 ± 0.015 0.039 ± 0.019 0.001 ± 0.001 0.019 ± 0.028 0.143 ± 0.023Table 8.4.2

- 4 (Corrected)

Interlaboratory comparison of juvenile growth and plasma vitellogenin titers following octylphenol exposure (mean ± SD). Highlighted cells a re significantly different than control

(α = 0.05).

Male Female

A B C A B C

Nominal OP concentration Weight (g) Weight (g)

0 ug/L 10.8 ± 1.7 10.1 ± 0.9 8.7 ± 1.2 11.9 ± 1.7 10.8 ± 1.3 8.3 ± 1 .0

6.25 ug/L 11.1 ± 0.6 10 .0 ± 0.4 8.9 ± 0.8 12.4 ± 0.4 11.4 ± 0.8 9.1 ± 1.1

12.5 ug/L 11.5 ± 1 .0 10.4 ± 0.4 7.6 ± 1.2 11.7 ± 1.2 10.6 ± 1.4 9.3 ± 0.8

25 ug/L 11.3 ± 0.7 10.8 ± 0.7 8.2 ± 0.7 12 .0 ± 1.4 9.2 ± 2.4 7.5 ± 0.2

50 ug/L 10.8 ± 0.7 10.2 ± 1.1 7.6 ± 1.1 11.6 ± 1.5 10.9 ± 2.8 7.5 ± 1.4

Length (mm) Length (mm)

0 ug/L 43 .0 ± 1.6 41.6 ± 1.7 41.1 ± 1.4 44.5 ± 2.7 42.4 ± 1.9 40.7 ± 2 .0

6.25 ug/L 44.1 ± 1.2 40.8 ± 0.4 41.6 ± 1.8 46.1 ± 1.7 43.6 ± 1 .0 42 .0 ± 1.7

12.5 ug/L 43.1 ± 1.4 42.5 ± 0.7 41.2 ± 1.8 44.3 ± 2.2 43.9 ± 2.5 43.8 ± 1.3

25 ug/L 43.4 ± 1.2 43.7 ± 0.5 42.1 ± 0.7 44.4 ± 2.2 41.5 ± 3.3 40.8 ± 0.3

50 ug/L 43.3 ± 1.1 41.7 ± 0.8 41 .0 ± 1.4 43.9 ± 1.6 43.5 ± 4 .0 40.1 ± 3.1

LSI LSI

0 ug/L 0.058 ± 0.005 0.058 ± 0.003 0.072 ± 0.004 0.058 ± 0.005 0.06 0 ± 0.006 0.075 ± 0.004

6.25 ug/L 0.06 0 ± 0.005 0.056 ± 0.004 0.065 ± 0.003 0.059 ± 0.004 0.059 ± 0.003 0.073 ± 0.004

12.5 ug/L 0.058 ± 0.002 0.058 ± 0.006 0.071 ± 0.003 0.058 ± 0.003 0.063 ± 0.002 0.075 ± 0.005

25 ug/L 0.062 ± 0.006 0.062 ± 0.003 0.066 ± 0.007 0.061 ± 0.006 0.056 ± 0.005 0.069 ± 0.002

50 ug/L 0.066 ± 0.01 0 0.058 ± 0.004 0.064 ± 0.007 0.062 ± 0.005 0.056 ± 0.002 0.068 ± 0.004

VTG (mg/mL) VTG (mg/mL)

0 ug/L 0.0004 ± 0.0004 0.013 0 ± 0.007 0 0.005 ± 0.001 0.0005 ± 0.0003 0.0074 ± 0.0042 0.037 ± 0.007

6.25 ug/L 0.0005 ± 0.0004 0.0092 ± 0.0063 0.007 ± 0.002 0.0004 ± 0.0005 0.0055 ± 0.0023 0.042 ± 0.011

12.5 ug/L 0.0014 ± 0.0007 0.0128 ± 0.0127 0.009 ± 0.002 0.0007 ± 0.0007 0.0146 ± 0.0119 0.056 ± 0.005

25 ug/L 0.0018 ± 0.0027 0.0074 ± 0.0032 0.02 0 ± 0.008 0.0005 ± 0.0002 0.0143 ± 0.0051 0.098 ± 0.01 0

50 ug/L 0.0017 ± 0.0011 0.015 0 ± 0.0069 0.033 ± 0.009 0.0008 ± 0.0004 0.0154 ± 0.0148 0.142 ± 0.01 0

# 17β

- trenbolone summary tables

The following summary tables are corrected to present the lowest observed effect levels based on the

corrected statistical analysis. They are presented in place of the tables found in section 5.3.3 of the

integrated summary report. Highlighted cells indicate a change in statistical outcome based on the

corrected analysis.

Summary of trenbolone growth endpoints.

All endpoints with lowest concentration statistically

different than control. Concentrations listed as

nominal regardless of mean measured concentrations.

Yellow highlights indicate where the LOEC increased

following the reanalysis. Dash: Not significant in e ither

analysis

.

Lab A

Nominal conc. 100, 50, 25, 12.5 ng/L

Larval sample

Weight 50 ng/L

Length

-

Juvenile Males

Weight

- Length

-

LSI

-

Juvenile Females

Weight

- Length

-

LSI

-



# Summary of trenbolone thyroid endpoints.

All endpoints with lowest concentration statistically

different than control. Concentrations listed as

nominal regardless of mean measured concentrations.

Reanalysis did not result in any changes. Dash: Not

significant in either analysis

.

Lab A

Nominal conc. 100, 50, 25, 12.5 ng/L

Larval sample

Time to NF62

-

Serum T 4 - Thyroid pathology 25 ng/L

# Summary of trenbolone reproductive endpoints.

All endpoints with lowest concentration statistically

different than control. Concentrations listed as nominal

regardless of mean measured concentrations. Yellow

highlights indicate where the LOEC increased following

the reanalysis. Dash: Not significant in either analysis

.

n.s. : Not significant after reanalysis.

Lab A

Nominal conc. 100, 50, 25, 12.5 ng/L

Juvenile Males

Plasma VTG n.s.

Gonad pathology 100 ng/L

Duct pathology 100 ng/L

Juvenile Females

Plasma VTG

- Gonad pathology

- Duct pathology 50 ng/L



# 17β

- trenbolone appendix data tables

The following two tables represent the same original data. The first table is from Appendix 8.4.3 of the

integrated summary report. The second table represents the correct analysis of the original data. In this

case there w as a difference in the statistical outcome. Orange shading ( ■ ) in the second table indicates

statistical significance in the corrected analysis.

Table 8.4.3

- 1 (Original)

Larval growth and serum thyroxine concentrations following trenbolone exposure at A (mean ± SD).

NF62 larvae

Nominal TB concentration Weight (g) Length (mm) Time to NF62 (d) T 4 ( ng/mL)

0 ug/L 0.818 ± 0.140 18.7 ± 1.1 40 ± 4 23.4 ± 9.0

12.5 ng/L 0.835 ± 0.146 18.7 ± 1.0 41 ± 4 25.0 ± 10.5

25 ng/L 0.853 ± 0.154 18.8 ± 1.2 40 ± 5 25.1 ± 9.7

50 ng/L 0.913 ± 0.193 19.2 ± 1.3 41 ± 6 30.6 ± 9.1

100 ng/L 0.867 ± 0.166 18.8 ± 1.2 39 ± 4 24.2 ± 9.8

Table 8.4.3

- 1 (Corrected)

Larval growth and serum thyroxine concentrations following trenbolone exposure at A (mean ± SD).

NF62 larvae

Nominal TB concentration Weight (g) Length (mm) Time to NF62 (d) T4 (ng/mL)

0 ug/L 0.818 ± 0.063 18.7 ± 0.6 40 ± 1 23.4 ± 9.0

12.5 ng/L 0.835 ± 0.030 18.7 ± 0.3 41 ± 1 25.0 ± 10.5

25 ng/L 0.853 ± 0.027 18.8 ± 0.4 40 ± 1 25.1 ± 9.7

50 ng/L 0.913 ± 0.077 19.2 ± 0.3 41 ± 1 30.6 ± 9.1

100 ng/L 0.867 ± 0.081 18.8 ± 0.8 39 ± 1 24.2 ± 9.8

# The following two tables represent the same original data. The first table is from Appendix 8.4.3 of the

integrated summary report. The second table represents the correct analysis of the original data. In this

case there were no differences in the statistical outcome.

Table 8.4.3

- 2 (Original)

Juvenile growth following trenbolone exposure at A (mean ± SD). Highlighted cells are significantly

different than control (α = 0.05).

Weight (g) Length (mm) LSI

Nominal TB concentration Female

0 ug/L 12.9 ± 3.7 47.5 ± 5.1 0.059 ± 0.006

12.5 ng/L 13.3 ± 2.6 47.3 ± 3.3 0.058 ± 0.010

25 ng/L 12.5 ± 3.2 46.4 ± 4.4 0.061 ± 0.006

50 ng/L 12.5 ± 3.1 46.7 ± 4.8 0.058 ± 0.008

100 ng/L 13.7 ± 3.6 49.0 ± 4.8 0.058 ± 0.009

Male

0 ug/L 12.3 ± 3.3 46.2 ± 4.1 0.06 ± 0.010

12.5 ng/L 11.4 ± 2.4 45.4 ± 3.2 0.058 ± 0.009

25 ng/L 11.9 ± 2.7 46.3 ± 4.0 0.057 ± 0.006

50 ng/L 13.0 ± 2.5 47.4 ± 4.0 0.057 ± 0.008

100 ng/L 11.7 ± 2.7 46.4 ± 3.6 0.058 ± 0.007

Table 8.4.3

- 2 (Corrected)

Juvenile growth following trenbolone exposure at A (mean ± SD). Highlighted cells are significantly

different than control (α = 0.05).

Weight (g) Length (mm) LSI

Nominal TB concentration Female

0 ug/L 12.9 ± 1.2 47.5 ± 1.9 0.059 ± 0.004

12.5 ng/L 13.3 ± 1.7 47.4 ± 2.4 0.058 ± 0.003

25 ng/L 12.5 ± 1.5 46.4 ± 1.8 0.061 ± 0.004

50 ng/L 12.3 ± 1.3 46.5 ± 2.7 0.058 ± 0.003

100 ng/L 13.7 ± 1.4 49.0 ± 1.4 0.058 ± 0.006

Male

0 ug/L 12.4 ± 1.2 46.3 ± 1.3 0.060 ± 0.004

12.5 ng/L 11.6 ± 1.5 45.7 ± 2.1 0.058 ± 0.005

25 ng/L 12.0 ± 1.5 46.3 ± 2.4 0.057 ± 0.004

50 ng/L 13.2 ± 0.4 47.6 ± 0.6 0.057 ± 0.004

100 ng/L 11.7 ± 1.2 46.4 ± 1.8 0.058 ± 0.003

# Benzophenone

- 2 summary tables

The following summary tables are corrected to present the lowest observed effect levels based on the

corrected statistical analysis. They are presented in place of the tables found in section 5.3.4 of the

integrated summary report. Highlighted cells indicate a change in statistical outcome based on the

corrected analysis.

Summary of BP

- 2 growth endpoints.

All endpoints with lowest concentration statistically

different than control. Concentrations listed as

nominal regardless of mean measured concentrations.

Yellow highlights indicate where the L OEC increased

following the reanalysis. Blue highlight indicates

where the LOEC decreased following reanalysis. n.s. :

Not significant after reanalysis

.

Lab E

Nominal conc. 6, 3, 1.5 mg/L

Larval sample

Weight 6 mg/L

Length 6 mg/L

Juvenile Males

Weight 6 mg/L

Length 6 mg/L

LSI n.s.

Juvenile Females

Weight 6 mg/L

Length 6 mg/L

LSI 3 mg/L



# Summary of BP

- 2 thyroid endpoints.

All endpoints with lowest concentration statistically

different than control. Concentrations listed as

nominal regardless of mean measured concentrations.

Reanalysis did not result in any changes.

Lab E

Nominal conc. 6, 3, 1.5 mg/L

Larval sample

Time to NF62 6 mg/L

Thyroid pathology 1.5 mg/L

# Summary of BP

- 2 reproductive endpoints.

All endpoints with lowest concentration statistically

different than control. Concentrations listed as

nominal regardless of mean measured concentrations.

Reanalysis did not result in any changes.

Lab E

Nominal

conc. 6, 3, 1.5 mg/L

Juvenile Males

Plasma VTG 1.5 mg/L

Gonad pathology 1.5 mg/L

Duct pathology 3 mg/L

Juvenile Females

Plasma VTG 1.5 mg/L

Gonad pathology 1.5 mg/L

Duct pathology 1.5 mg/L



# Benzophenone

- 2 appendix data tables

The following two tables represent the same original data. The first table is from Appendix 8.4.4 of the

integrated summary report. The second table represents the correct analysis of the original data. In this

case there were no differences in the stat istical outcome. Yellow shading ( ■ ) indicates statistical

significance with the previous incorrect analysis. Orange shading ( ■ ) in the second table indicates

statistical significance in the corrected analysis.

Table 8.4.4

- 3 (Original)

Larval growth and serum thyroxine concentrations following BP2 exposure at E (mean ± SD).

Highlighted cells are significantly different than control (α = 0.05).

NF62 larvae

Nominal TB concentration Weight (g) Length (mm) Time to NF62 (d)

0 mg/L 1.18 ± 0.18 22.2 ± 1.1 38 ± 5

1.5 mg/L 1.05 ± 0.23 21.5 ± 1.3 37 ± 3

3 mg/L 1.18 ± 0.18 22.2 ± 1.3 38 ± 3

6 mg/L 1.92 ± 0.59 25.2 ± 2.1 49 ± 7

Table 8.4.4

- 3 (Corrected)

Larval growth and serum thyroxine concentrations following BP2 exposure at E (mean ± SD).

Highlighted cells are significantly different than control (α = 0.05).

NF62 larvae

Nominal TB concentration Weight (g) Length (mm) Time to NF62 (d)

0 mg/L 1.1 8 ± 0.09 22. 2 ± 0.5 37 ± 1

1.5 mg/L 1.05 ± 0.08 21. 5 ± 0.4 37 ± 1

3 mg/L 1.1 8 ± 0. 10 22.2 ± 0.8 3 8 ± 1

6 mg/L 1.9 3 ± 0.15 25.3 ± 0.8 48 ± 1

# The following two tables represent the same original data. The first table is from Appendix 8.4.4 of the

integrated summary report. The second table represents the correct analysis of the original data. In this

case there were differences in the statistical outcome. Yellow shading ( ■ ) indicates statistical

significance with t he previous incorrect analysis. Orange shading ( ■ ) in the second table indicates

statistical significance in the corrected analysis.

Table 8.4.4

- 4 (Original)

Juvenile growth and plasma vitellogenin titers following BP2 exposure at E (mean ± SD). Highlighted cells are

significantly different than control (α = 0.05).

Weight (g) Length (mm) LSI VTG (mg/mL)

Nominal BP2 concentration Female

0 mg/L 10.8 ± 2.1 44.7 ± 3.2 0.045 ± 0.006 0.04 ± 0.11

1.5 mg/L 11.8 ± 3.1 47.4 ± 4.4 0.052 ± 0.006 60.2 ± 27.2

3 mg/L 9.5 ± 4.8 43.3 ± 7.3 0.057 ± 0.013 245.8 ± 61.1

6 mg/L 4.1 ± 1.2 33.4 ± 2.7 0.036 ± 0.009 250.9 ± 75.5

Male

0 mg/L 11.3 ± 2.7 45.2 ± 3.3 0.046 ± 0.005 0.02 ± 0.03

1.5 mg/L 11.1 ± 2.6 46.7 ± 4.1 0.052 ± 0.006 59.9 ± 23.5

3 mg/L 9.8 ± 5.5 43.0 ± 7.5 0.055 ± 0.018 232.9 ± 56.9

6 mg/L 4.4 ± 1.4 33.9 ± 3.5 0.035 ± 0.007 259.4 ± 35.6

Table 8.4.4

- 4 (Corrected)

Juvenile growth and plasma vitellogenin titers following BP2 exposure at E (mean ± SD). Highlighted cells are

significantly different than control (α = 0.05).

Weight (g) Length (mm) LSI VTG (mg/mL)

Nominal BP2 concentration Female

0 mg/L 10.8 ± 1.1 44.7 ± 1. 8 0.045 ± 0.003 0.04 ± 0.06

1.5 mg/L 11. 9 ± 1.9 47.4 ± 2.9 0.052 ± 0.003 60.2 ± 11.0

3 mg/L 9.5 ± 2.2 43. 3 ± 3. 5 0.057 ± 0.004 245.8 ± 25.6

6 mg/L 4.1 ± 0. 8 33.4 ± 1.6 0.034 ± 0.007 249.5 ± 54.8

Male

0 mg/L 11. 3 ± 1.4 45. 2 ± 1. 3 0.046 ± 0.003 0.02 ± 0.02

1.5 mg/L 11. 2 ± 1. 4 46. 7 ± 2.1 0.052 ± 0.001 59.9 ± 7.0

3 mg/L 9.5 ± 3.7 42.5 ± 5. 1 0.054 ± 0.011 236.7 ± 43.6

6 mg/L 4.6 ± 1.1 34. 3 ± 2. 2 0.036 ± 0.004 257.0 ± 15.6

# Control comparisons

The following two tables are corrected means ( ± SD ) and coefficients of variation. These tables are

presented in place of tables 6.1

- 1 and 6.1

- 2 in the integrated summary report. There are m inor differences

in means between the previous and corrected tables but there are sizable differences in the standard

deviations.

Table 6.1

- 1 (Corrected)

Inter

- laboratory control larvae growth and development performance comparison. Entries are listed in descending order

according to weight (mean ± SD).

Study Weight (g) CV Length (mm) CV Time to NF62 (d) CV

A

- PZ

- - -

B

- OP 2.07 ± 0.17 8 23.1 ± 0.5 2 42 ± 2 5

E

- BP2 1.18 ± 0.09 8 22. 2 ± 0.5 2 37 ± 1 2

B

- PZ 1.08 ± 0.11 10 20.8 ± 0.5 2 4 1 ± 2 5

A

- OP 0.96 ± 0.08 8 19.2 ± 0.5 3 42 ± 1 2

A

- TB 0.8 2 ± 0.06 8 18.7 ± 0.6 3 40 ± 1 3

D

- PZ 0.79 ± 0.05 6 18.8 ± 0.3 2 43 ± 1 3

C

- PZ 0.62 ± 0.0 8 12 17.1 ± 0.7 4 44 ± 3 6

C

- OP 0.46 ± 0.03 7 15.2 ± 0.4 3 41 ± 3 7

Overall means 1.0 ± 0.49 49 19.4 ± 2.6 13 41 ± 2 4



# Table 6.1

- 2 (Corrected)

Interlaboratory control juvenile growth performance and vitellogenin titer comparison. Entries are listed in descending orde r according to weight (mean ± SD).

Weight (g) CV Length (mm) CV LSI CV VTG (mg/mL) CV

Study Male

D

- PZ 18.3 ± 1.5 8 52.9 ± 1 .0 2 0.058 ± 0.002 3 0.012 ± 0.01 0 83

A

- PZ 13.3 ± 1.1 8 47.4 ± 1.5 3 0.059 ± 0.007 12 0.033 ± 0.013 39

A

- TB 12.4 ± 1.2 10 46. 3 ± 1. 3 3 0.06 0 ± 0.004 7 0.069 ± 0.024 35

B

- PZ 11.7 ± 1.3 11 43.3 ± 2.0 5 0.057 ± 0.004 7 0.002 ± 0.003 150

E

- BP2 11.3 ± 1.4 13 45. 2 ± 1. 3 3 0.046 ± 0.003 7 0.02 0 ± 0.02 0 100

A

- OP 10.8 ± 1.7 16 43 .0 ± 1.6 4 0.058 ± 0.005 9 0.0004 ± 0.0004 100

B

- OP 10.1 ± 0.9 9 41.6 ± 1.7 4 0.058 ± 0.003 5 0.013 ± 0.007 54

C

- OP 8.7 ± 1.2 14 41.1 ± 1.4 3 0.072 ± 0.004 6 0.005 ± 0.001 20

C

- PZ 4 .0 ± 0.2 5 32.9 ± 0.7 2 0.034 ± 0.005 15 0.005 ± 0.002 40

Overall ♂ means 11.2 ± 3.8 34 43.7 ± 5.4 12 0.056 ± 0.010 19 0.018 ± 0.022 123

Female

D

- PZ 19.9 ± 2.6 13 55 .0 ± 2.6 5 0.059 ± 0.003 5 0.019 ± 0.032 168

B

- PZ 13.7 ± 3.1 23 46.5 ± 2.8 6 0.067 ± 0.01 0 15 0.006 ± 0.009 150

A

- PZ 12.9 ± 0.7 5 47.2 ± 0.9 2 0.061 ± 0.003 5 0.033 ± 0.016 48

A

- TB 12. 9 ± 1.2 9 47.5 ± 1.9 4 0.059 ± 0.004 7 0.063 ± 0.041 65

A

- OP 11.9 ± 1.7 14 44.5 ± 2.7 6 0.058 ± 0.005 9 0.0005 ± 0.0003 60

B

- OP 10.8 ± 1.3 12 42.4 ± 1.9 4 0.06 0 ± 0.006 10 0.007 ± 0.004 57

E

- BP2 10. 8 ± 1.1 10 44.7 ± 1. 8 4 0.045 ± 0.003 7 0.04 0 ± 0.06 0 150

C

- OP 8.3 ± 1 .0 12 40.7 ± 2 .0 5 0.075 ± 0.004 5 0.037 ± 0.007 19

C

- PZ 4.1 ± 0.5 12 32.8 ± 1.4 4 0.032 ± 0.004 13 0.03 0 ± 0.006 20

Overall ♀ means 11.7 ± 4.3 36 44.6 ± 6.0 13 0.057 ± 0.012 22 0.026 ± 0.020 76