EXHIBIT 31.1 CERTIFICATION PURSUANT TO RULE 13A-14(A) 
AS ADOPTED PURSUANT TO SECTION 302 OF 
THE SARBANES-OXLEY ACT OF 2002 I, Kuslima
Shogen, certify that: 1.
I have
 reviewed this quarterly report on Form 10-Q of Alfacell Corporation;2.
Based on my
 knowledge, this report does not contain any untrue statement of a material
 fact or omit to state a material fact necessary to make the statements made,
 in light of the circumstances under which such statements were made, not
 misleading with respect to the period covered by this report;3.
Based on my
 knowledge, the financial statements, and other financial information included
 in this report, fairly present in all material respects the financial
 condition, results of operations and cash flows of the registrant as of, and
 for, the periods presented in this report;4.
I am
 responsible for establishing and maintaining disclosure controls and
 procedures (as defined in Exchange Act Rules 13a-15(e) and 15d-15(e)) and
 internal control over financial reporting (as defined in Exchange Act Rules
 13a-15(f) and 15d-15(f)) for the registrant and have:  (a)
Designed
 such disclosure controls and procedures, or caused such disclosure controls
 and procedures to be designed under my supervision, to ensure that material
 information relating to the registrant is made known to me by others within
 those entities, particularly during the period in which this report is being
 prepared;(b)
Designed
 such internal control over financial reporting, or caused such internal
 control over financial reporting to be designed under my supervision to
 provide reasonable assurance regarding the reliability of financial reporting
 and the preparation of financial statements for external purposes in
 accordance with generally accepted accounting principles;(c)
Evaluated
 the effectiveness of the registrants disclosure controls and procedures and
 presented in this report my conclusions about the effectiveness of the
 disclosure controls and procedures, as of the end of the period covered by
 this report based on such evaluation; and(d)
Disclosed in
 this report any change in the registrants internal control over financial
 reporting that occurred during the registrants most recent fiscal quarter
 (the registrants fourth fiscal quarter in the case of an annual report) that
 has materially affected, or is reasonably likely to materially affect, the
 registrants internal control over financial reporting; and  5.
I have
 disclosed, based on my most recent evaluation of internal control over
 financial reporting, to the registrants auditors and the audit committee of
 the registrants board of directors (or persons performing the equivalent
 functions):  (a)
All
 significant deficiencies and material weaknesses in the design or operation
 of internal control over financial reporting which are reasonably likely to
 adversely affect the registrants ability to record, process, summarize and
 report financial information; and(b)
Any fraud,
 whether or not material, that involves management or other employees who have
 a significant role in the registrants internal control over financial
 reporting.  Date: March
 12, 2009/s/ Kuslima
 ShogenName:
Kuslima ShogenTitle:
Chief
 Executive Officer and Acting Chief Financial Officer (Principal Executive
 Officer, Principal Accounting Officer and Principal Financial Officer)