Effluent Quality Assessment 

Florida Power and Light Company 

Fort Myers Plant 

Lee County 

NPDES #FL0001490 

Sampled July 21, 2008 

November 2008

Biology Section 

Bureau of Laboratories 

Division of Environmental Assessment and Restoration 

Quality Manual No. 870346G 

NELAC Certification No. E31780
1

Florida Power and Light Company – Fort Myers Plant, 10650 State Road 80, Fort Myers, Lee 

County, Florida, 33905. NPDES #FL0001490. Effluent samples for this facility were collected on 

July 21, 2008. 

Introduction 

Florida Power and Light Company – Fort Myers Plant is an electric power generating plant. The 

plant consists of: 

• six natural gas combined-cycle combustion turbines with a total generating capacity of 

1,550 megawatts (MW); 

• associated Heat-Recover Steam Generators and steam turbines; 

• twelve gas turbine peaking units with a combined generating capacity of 600 (MW); 

• two peaker combustion turbines with a combined generating capacity of 326 (MW); 

• a once-through condenser cooling water system; 

• a once-through helper cooling tower; 

• a stormwater basin; 

• an evaporation/percolation pond; 

• ground water monitoring wells; and, 

• a reverse osmosis plant. 

The facility does not have a design flow. The combined discharge (once through cooling water, 

RO plant discharge, auxiliary equipment cooling water, etc.) from the plant from July 2007 

through June 2008 was approximately 670.3 million gallons per day (MGD). Surface water 

discharge occurs at the end of the 762 M (2,500 feet) long on-site discharge canal at Outfall D010. The receiving water is the Class III Marine waters of the Orange River (Note: the Orange 

River is designated as Class III freshwater in the facility’s permit; however, watershed biologists 

classify this portion of the river as tidal marine). The point of discharge is located approximately at 

latitude 26o 41’ 31” N, longitude 81o 46’ 41” W. Wastewater treatment at the facility consists of 

routing equipment-area stormwater and washdown water that have the potential to contain oil and 

grease through an oil/water separator prior to discharge to the stormwater basin. The contents of 

the stormwater basin are pumped to the evaporation/percolation basin. Fuel oil storage runoff is 

administratively controlled by visual inspections prior to discharge to the evaporation/percolation 

basin. Metal cleaning wastewater is disposed of offsite. 

The facility’s once-through condenser cooling water system uses water from the Caloosahatchee 

River as intake water. Cooling water is routed through the plant condensers and is then discharged 

to the discharge canal. A once-through cooling tower, located adjacent to the discharge canal, is 

used to reduce the discharge temperature of the cooling water. Boiler blowdown is captured and 

reused. Reverse osmosis reject is discharged indirectly to the discharge canal via an internal 

discharge to the cooling tower basin. 

The facility has no permitted toxicity test requirements; however, a 48-hour acute screening test 

can provide confidence that the effluent meets the acute toxicity criterion of Florida’s Surface 

Water Quality Standards (Rule 62-302.500 (1)(a)4, F.A.C.) if the mortality in 100% effluent is less 

than 20%. 

The facility has no Administrative Orders, Consent Orders or mixing zones. A review of Discharge 

Monitoring Report data showed no permit violations for the past year. (Facility information from 

NPDES permit #FL0001490 and Alyssa Mork FDEP South District.) 

Methods 

Samples were collected from the EFF-1 NPDES sampling point for Outfall D-010 by Alyssa Mork 

(FDEP South District) following DEP-SOP-001/01 FS 2400 Wastewater Sampling. All DEP SOPs 

are available on the web at: http://www.floridadep.org/labs/qa/sops.htm. 
2

See Appendix A for the completed chain of custody form. 

The 48 - hour acute screening toxicity tests and algal growth potential test were performed 

following internal DEP SOPs TA07_01, TA07_02, and TA08_01 through TA08_09. All internal 

DEP SOPs are available on the web at: http://www.floridadep.org/labs/cgi-bin/sop/biosop.asp. 

An aliquot of the AGP sample was salinity adjusted to meet the minimum salinity requirements of 

the marine test species. Both a freshwater AGP analysis and a marine AGP analysis were 

performed. 

Toxicity Test Results 

Tests were performed July 22 - 24, 2008. 

Ceriodaphnia dubia 48-hour acute screening bioassay – LC50 > 100%, 0% mortality* in 100% 

 sample at 48 hours 

Cyprinella leedsi 48-hour acute screening bioassay – LC50 > 100%, 5% mortality* in 100% 

 sample at 48 hours 

The bioassay samples were not acutely toxic to the test organisms. 

See Appendix B for bioassay bench sheets. See Appendix C for results of the last 20 standard 

reference toxicant (SRT) tests and the bench sheets and statistical analyses of the SRT tests that 

correspond to these facility tests. See Appendix D for test organism receipts. 

*NOTE: In a 48-hour acute screening test of 100% effluent, mortality of <20% provides 

confidence that the effluent meets the acute toxicity criterion of Florida’s Surface Water Quality 

Standards (Rule 62-302.500 (1)(a)4, F.A.C.). Mortality between 20-50% indicates low to 

moderate levels of toxicity, and further action may be required. Mortality of >50% indicates that 

the effluent fails to meet the minimum requirement to discharge to waters of the state (Rule 62-

4.244(3)(a), F.A.C.). 

Algal Growth Potential 

The effluent Algal Growth Potential (AGP) was 24.9 mg dry wt/L of the unicellular green alga, 

Pseudokirchneriella subcapitata, formerly known as Selenastrum capricornutum. Raschke and 

Shultz (1987) found that AGP values above 5.0 mg dry weight/L represent a “problem” threshold 

for fresh receiving waters, implying nutrient enrichment. The analytical chemistry suggests that 

the effluent is nitrogen-limited. The predicted AGP based on the effluent’s inorganic nitrogen 

concentration was 10.3 (±20%) mg dry wt/L (Table 1; Miller et al. 1978). 

The salinity-adjusted effluent AGP was 3.0 U (U - Material was analyzed for but not detected; the 

reported value is the minimum detection limit) mg dry wt/L of the saltwater species, Dunaliella 

tertiolecta. Raschke and Shultz found that AGP values above 10.0 mg dry weight/L represent a 

“problem” threshold for marine receiving waters, implying nutrient enrichment (personal 

communication, Marshall Faircloth, et. al. FDEP, with Ron Raschke USEPA Region 4 1987). One 

potential reason for the lack of growth in the salinity-adjusted sample is precipitation of enough 

orthophosphate by the added salts that the sample became extremely phosphorus-limited 

(Manahan 1994). 

See Table 1 for AGP results 
3

Chemistry Results 

Total residual chlorine (TRC) and total ammonia were not detected in the bioassay sample in the 

laboratory. TRC was not detected in the effluent in the field. The total ammonia concentration in 

the sample collected and preserved for chemical analysis was 0.1 mg/L Y (Y – the laboratory 

analysis was from an unpreserved or improperly preserved sample; the data may not be 

accurate) mg/L. Based on the pH, salinity, and temperature of the effluent as collected, the 

calculated unionized ammonia concentration was ≤ 0.02 mg/L. 

Aluminum, arsenic, copper, nickel and norflurazon were detected in the effluent at levels that 

comply with Class III marine water quality criteria (62-302.530, F.A.C.). Bromacil, chromium III 

and lead were detected between the laboratory method detection limits (MDL) and practical 

quantitation limits (PQL). 

The effluent iron (430 μg/L) violated the Class III marine water criterion for iron (≤ 300 μg/L; 62-

302.530(39), F.A.C.). 

See Table 2 for results of analytes detected in the effluent, and corresponding limits. See 

Appendix E for a complete list of chemical analyses performed. 

Conclusion 

The effluent sample collected from the EFF-1 NPDES sampling point for Outfall D-010 for this 

facility on July 21, 2008, was not acutely toxic to either test species during the 48-hour acute 

screening bioassays. The effluent AGP result exceeded the "problem” threshold for fresh 

receiving waters. The AGP in the salinity-adjusted effluent was less than the method detection 

limit. Effluent iron violated the Class III marine water criterion for iron. The measured constituents 

of other effluent water quality samples did not exceed any applicable water quality standards or 

violate any permit conditions. 

Literature Cited 

Miller, W. E., T. E. Maloney, and J. C. Greene. 1978. The Selenastrum capricornutum Printz 

algal assay bottle test. EPA-600/9-78-018. U. S. EPA, Cincinnati, Ohio. 126 p. 

Manahan, S. E. 1994. Environmental Chemistry, 6th edition. Lewis Publishers, USA. 811 pp 

Raschke, R. L. and D. A. Schultz. 1987. The use of the algal growth potential test for data 

assessment. J. Wat. Poll. Cont. Fed. 59(4): 222-227. 
4

Florida Power and Light Company - Fort Myers Plant

Inorganic Total

Sample N:P ratio N:P ratio

Efluent 24.9 10.3 ± 2.1 74.9 ± 15.0 1.3 6.6

Table 1a. Measured and predicted algal growth potential (AGP; mg dry weight/L) for total soluble 

inorganic nitrogen (TSIN) and total nitrogen (TN) limitation of the freshwater species 

Pseudokirchneriella subcapitata, and ratios of nitrogen to phosphorus for samples collected from the 

Florida Power and Light Company - Fort Myers Plant on July 21, 2008.

AGP

(measured)

Predicted AGP

(TSIN) ± 20%

Predicted AGP

(TN) ± 20%

Florida Power and Light Company - Fort Myers Plant

Inorganic Total

N:P ratio N:P ratio

Efluent 3.0 U 1.3 6.6

Table 1b. Measured algal growth potential (AGP; mg dry weight/L) 

of the saltwater species Dunaliella tertiolecta and ratios of 

nitrogen to phosphorus for samples collected from the Florida 

Power and Light Company - Fort Myers Plant on July 21, 2008.

AGP

(measured)

U - Material analyzed for but not detected; value reported is the method 

detection limit

Salinity-adjusted 

Sample
5

Florida Power and Light Company - Fort 

Myers Plant NPDES# FL0001490 Class III Criteria Effluent Limits

Bromacil - - 0.59 IJ

Norflurazon - - 1.4

Aluminum ≤ 1500 - 260

Arsenic ≤ 50 - 2.2

Cadmium ≤ 8.8 - 0.02 U

Calcium (mg/L) - - 59.8

Chromium-III - - 0.68 I

Copper ≤ 3.7 - 3.1

Iron ≤ 300 - 430

Lead ≤ 8.5 - 0.29 I

Magnesium (mg/L) - - 13.4

Nickel ≤ 8.3 - 1.01

Selenium ≤ 71 - 0.5 U

Silver ≤ 2.3 - 0.025 U

Zinc ≤ 86 - 5 U

Ortho-phosphate - - 0.21 Y

Total Phosphorus - - 0.3 Y

Total Ammonia - - 0.1 Y

Un-ionized Ammonia - - ≤ 0.02 c

Nitrate and Nitrite - - 0.17 Y

Total Kjeldahl Nitrogen - - 1.8 Y

Organic Nitrogen - - 1.7 c

Total Nitrogen - - 1.97 c

Dissolved Oxygen (mg/L) ≥ 4.0 s - 4.9

pH (SU) 6.5 - 8.5 s 6.5 - 8.5 s 7.5

Conductivity (μmhos/cm) - - 0.34

Temperature °C - Report 35.7

Sample Depth (m) - - 1

Total Residual Oxidants (mg/L) ≤ 0.01 s ≤ 0.01 s 0

Chlorophyll a (μg/L) - Corrected - - 8.6 AY

Phaeophytin (μg/L) - - 3.1 AY

CBOD, 5 day (mg/L), N - inhibited - - 1.5 AIY

Oils and Greases (mg/L) ≤ 5.0 s - 1.8 I

Flow (MGD) - - 670.3 a

Hardness (mg CaCO3) - - 204.50 c

Fecal Coliforms ≤ 400s # - 28 B

Value exceeds the Class III Water Quality Criteria or permit limits

a - Annual average

c - Value is calculated

s - Single sample

A - Value reported is the mean of two or more determinations

B - Results based on colony counts outside the acceptance range

I - The reported value is between the laboratory method detection limit and the laboratory practical quantitation limit

J - Estimated value

U - Material analyzed for but not detected; value reported is the method detection limit

Y - The laboratory analysis was from an unpreserved or improperly preserved sample. The data may not be accurate.

 # - If 10 or fewer samples are taken within a month, the single sample limit is 400 CFU (62-302.530(6), F.A.C.)

Table 2. Effluent limits, Class III Criteria for predominantly marine waters and chemical and 

microbiological data for samples collected from the Florida Power and Light Company - Fort 

Myers Plant on July 21, 2008.

General Physical and Chemical Parameters

Microbiology (Number of colony forming units/100ml)

Efluent

Organic Constituents (μg/L)

Metals (μg/L unless otherwise noted)

Nutrients (mg/L)
6

Appendix A. Chain of Custody Form 
7

Appendix B. Bench Sheets 
8

Appendix B. (continued) 
9

Appendix B. (continued) 
10

Appendix B. (continued) 
11

Appendix B. (continued) 
12

Appendix B. (continued) 
13

Appendix C. SRT Bench Sheets and Statistical Analysis 

Florida Department of Environmental Protection

Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - 48-hour Acute Toxicity

Ceriodaphnia dubia (water flea) - Sodium Chloride (NaCl)

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit

01/16/07 2.22 2.19 0.17 1.85 2.52

02/13/07 2.39 2.31 0.17 1.97 2.54

03/13/07 1.92 2.18 0.18 1.82 2.55

04/03/07 2.37 2.20 0.18 1.83 2.57

05/08/07 1.78 2.18 0.21 1.77 2.59

05/29/07 1.69 2.06 0.23 1.70 2.60

06/06/07 2.28 2.16 0.22 1.71 2.60

07/03/07 1.99 2.14 0.22 1.70 2.58

07/31/07 1.94 2.14 0.22 1.71 2.58

09/04/07 1.95 2.13 0.22 1.70 2.57

10/02/07 2.04 2.11 0.21 1.68 2.53

10/30/07 2.35 2.11 0.21 1.68 2.54

12/04/07 2.13 2.09 0.20 1.67 2.51

01/22/08 1.92 2.08 0.20 1.66 2.50

02/05/08 2.25 2.08 0.20 1.68 2.48

03/04/08 2.15 2.08 0.20 1.68 2.49

04/01/08 2.50 2.11 0.22 1.68 2.55

05/06/08 2.19 2.11 0.22 1.68 2.55

06/03/08 1.91 2.10 0.22 1.66 2.54

07/08/08 2.21 2.11 0.22 1.67 2.55

Means are of the last 20 SRT tests.

Lower Control Limit = mean - 2 * Std Dev CV = 10.35

Upper Control Limit = mean + 2 * Std Dev EPA reference 75th quartile CV = 29%

Standard Reference Toxicant Data 

Ceriodaphnia dubia 48 hour LC50

1.50

2.00

2.50

3.00

01/16/0702/13/0703/13/0704/03/0705/08/0705/29/0706/06/0707/03/0707/31/0709/04/0710/02/0710/30/0712/04/0701/22/0802/05/0803/04/0804/01/0805/06/0806/03/0807/08/08

Test Date

g/L NaCl

LC50 Mean LC50 Lower Control Limit Upper Control Limit
14

Appendix C. (continued) 

Florida Department of Environmental Protection

Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - 48-hour Acute Toxicity

Cyprinella leedsi (bannerfin shiner) - Sodium Chloride (NaCl)

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit

01/16/07 8.47 8.04 0.47 7.11 8.98

02/14/07 8.92 8.70 0.32 7.12 9.10

03/14/07 7.75 8.38 0.59 7.16 9.09

04/03/07 8.50 8.41 0.49 7.19 9.12

05/08/07 9.07 8.22 0.52 7.18 9.25

05/30/07 8.85 8.59 0.48 7.23 9.32

07/03/07 8.67 8.33 0.50 7.34 9.32

07/10/07 8.52 8.36 0.49 7.38 9.34

08/01/07 7.7 8.31 0.51 7.29 9.34

09/05/07 9.06 8.34 0.54 7.26 9.42

10/02/07 8.55 8.43 0.47 7.45 9.35

10/30/07 8.72 8.43 0.47 7.48 9.37

12/04/07 7.71 8.36 0.47 7.41 9.31

01/08/08 8.7 8.35 0.47 7.41 9.30

02/05/08 8.92 8.42 0.46 7.50 9.33

03/04/08 7.89 8.42 0.46 7.50 9.33

04/01/08 8.24 8.42 0.45 7.51 9.33

05/15/08 8.1 8.39 0.51 7.36 9.41

06/03/08 8.21 8.37 0.51 7.34 9.40

07/08/08 8.04 8.33 0.50 7.32 9.33

Means are of the last 20 SRT tests.

Lower Confidence Limit = mean - 2 * Std Dev CV = 6.03

Upper Confidence Limit = mean + 2 * Std Dev EPA reference 75th quartile CV = 19%

Standard Reference Toxicant Data 

Cyprinella leedsi 48-hour LC50

4

6

8

10

01/16/0702/14/0703/14/0704/03/0705/08/0705/30/0707/03/0707/10/0708/01/0709/05/0710/02/0710/30/0712/04/0701/08/0802/05/0803/04/0804/01/0805/15/0806/03/0807/08/08

Test Date

g/L NaCl

LC50 Mean LC50 Lower Control Limit Upper Control Limit
15

Appendix C. (continued) 
16

Appendix C. (continued) 
17

Appendix C. (continued) 
18

Appendix C. (continued) 
19

Appendix C. (continued) 

Ceriodaphnia dubia Acute Standard Reference Toxicant Test 

DATE: 7-8-08 DURATION: 48 hr TOXICANT : NaCl 

SPECIES: Ceriodaphnia dubia

 RAW DATA: Concentration Number Mortalities 

 (g/L) Exposed 

 .00 20 0 

 1.50 20 0 

 2.00 20 1 

 2.50 20 20 

 3.00 20 20 

 4.00 20 20 

 SPEARMAN-KARBER TRIM: .00% 

 SPEARMAN-KARBER ESTIMATES: LC50: 2.21

 95% LOWER CONFIDENCE: 2.15 

 95% UPPER CONFIDENCE: 2.26 

__________________________________________________________ 

Cyprinella leedsi Acute Standard Reference Toxicant Test 

DATE: 7-8-08 DURATION: 48 hr TOXICANT : NaCl 

SPECIES: Cyprinella leedsi 

 RAW DATA: Concentration Number Mortalities 

 (g/L) Exposed 

 0.00 20 0 

 6.00 20 2 

 7.00 20 3 

 8.00 20 9 

 9.00 20 16 

 10.00 20 20 

 SPEARMAN-KARBER TRIM: 0.00% 

 SPEARMAN-KARBER ESTIMATES: LC50: 8.04

 95% LOWER CONFIDENCE: 7.64 

 95% UPPER CONFIDENCE: 8.47 
20

Appendix D. Test Organism Receipt(s) 
21

Appendix D. (continued) 
22

Appendix E. Chemical Analyses performed on the effluent from the Florida Power and Light-FoOutfall D-010, sampled on July 21, 2008.

Date Sampled Field ID Analysis Component Result Remark Units MDL PQ

7/21/2008 11:30 Efluent AGP/LN Algal Growth Potential 24.9 mg DryWt/L 0.3 0.7/21/2008 11:30 Efluent AGP/LN/SA Algal Growth Potential Salinity-adjusted 3 U mg DryWt/L 3 3 7/21/2008 11:30 Efluent Chlorophyll/Grain Size/BOD Chlorophyll-a, Corrected 8.6 AY ug/L 0.55 1.7/21/2008 11:30 Efluent Chlorophyll/Grain Size/BOD Biochemical Oxygen Demand-5 Day,N-Inhib 1.5 AIY mg/L 0.2 2 7/21/2008 11:30 Efluent Chlorophyll/Grain Size/BOD Phaeophytin-a 3.1 AY ug/L 0.24 0.7/21/2008 11:30 Efluent Overflow Fecal Coliforms-Membrane Filter 28 B #/100 mL 7/21/2008 11:30 Efluent Toxicology Bioassay-Acute-Screen-FW-C.dubia, LC50 100 L LC50 7/21/2008 11:30 Efluent Toxicology Bioassay-Acute-Screen-FW-Fish, LC50 100 L LC50 7/21/2008 11:30 Efluent Metals Chromium 0.68 I ug/L 0.5 2 7/21/2008 11:30 Efluent Metals Aluminum 260 ug/L 60 247/21/2008 11:30 Efluent Metals Calcium 59.8 mg/L 0.075 0.7/21/2008 11:30 Efluent Metals Magnesium 13.4 mg/L 0.04 0.7/21/2008 11:30 Efluent Metals Arsenic 2.2 ug/L 0.5 2 7/21/2008 11:30 Efluent Metals Zinc 5 U ug/L 5 20 7/21/2008 11:30 Efluent Metals Silver 0.025 U ug/L 0.025 0.7/21/2008 11:30 Efluent Metals Copper 3.1 ug/L 0.5 2 7/21/2008 11:30 Efluent Metals Iron 430 ug/L 30 127/21/2008 11:30 Efluent Metals Cadmium 0.02 U ug/L 0.02 0.7/21/2008 11:30 Efluent Metals Selenium 0.5 U ug/L 0.5 2 7/21/2008 11:30 Efluent Metals Antimony 0.5 U ug/L 0.5 2 7/21/2008 11:30 Efluent Metals Lead 0.29 I ug/L 0.2 0.7/21/2008 11:30 Efluent Metals Nickel 1.01 ug/L 0.25 1 7/21/2008 11:30 Efluent Metals Beryllium 0.025 U ug/L 0.025 0.7/21/2008 11:30 Efluent Nutrients Total-P 0.3 Y mg P/L 0.02 0.7/21/2008 11:30 Efluent Nutrients Kjeldahl Nitrogen 1.8 Y mg N/L 0.08 0.7/21/2008 11:30 Efluent Nutrients O-Phosphate-P 0.21 Y mg P/L 0.004 0.7/21/2008 11:30 Efluent Nutrients NO2NO3-N 0.17 Y mg N/L 0.004 0.7/21/2008 11:30 Efluent Nutrients Ammonia-N 0.1 Y mg N/L 0.01 0.7/21/2008 11:30 Efluent Overflow Oil and Grease 1.8 I mg/L 1.4 5 7/21/2008 11:30 Efluent Pesticides Ametryn 0.048 U ug/L 0.048 0.7/21/2008 11:30 Efluent Pesticides Atrazine 0.048 U ug/L 0.048 0.7/21/2008 11:30 Efluent Pesticides Metalaxyl 0.24 U ug/L 0.24 0.7/21/2008 11:30 Efluent Pesticides Norflurazon 1.4 ug/L 0.14 0.
23

Appendix E. (continued)

Date Sampled Field ID Analysis Component Result Remark Units MDL PQ

7/21/2008 11:30 Efluent Pesticides Ethion 0.048 U ug/L 0.048 0.7/21/2008 11:30 Efluent Pesticides Malathion 0.14 U ug/L 0.14 0.7/21/2008 11:30 Efluent Pesticides Phorate 0.048 UJ ug/L 0.048 0.7/21/2008 11:30 Efluent Pesticides Alachlor 0.58 U ug/L 0.58 2.7/21/2008 11:30 Efluent Pesticides Chlorpyrifos Ethyl 0.048 U ug/L 0.048 0.7/21/2008 11:30 Efluent Pesticides Hexazinone 0.096 U ug/L 0.096 0.7/21/2008 11:30 Efluent Pesticides Azinphos Methyl 0.19 U ug/L 0.19 0.7/21/2008 11:30 Efluent Pesticides Butylate 0.19 U ug/L 0.19 0.7/21/2008 11:30 Efluent Pesticides Chlorpyrifos Methyl 0.096 U ug/L 0.096 0.7/21/2008 11:30 Efluent Pesticides Mevinphos 0.19 UJ ug/L 0.19 0.7/21/2008 11:30 Efluent Pesticides Naled 0.77 U ug/L 0.77 3.7/21/2008 11:30 Efluent Pesticides Diazinon 0.048 U ug/L 0.048 0.7/21/2008 11:30 Efluent Pesticides Ethoprop 0.096 UJ ug/L 0.096 0.7/21/2008 11:30 Efluent Pesticides Simazine 0.048 U ug/L 0.048 0.7/21/2008 11:30 Efluent Pesticides Bromacil 0.59 IJ ug/L 0.19 0.7/21/2008 11:30 Efluent Pesticides Prometryn 0.14 U ug/L 0.14 0.7/21/2008 11:30 Efluent Pesticides Fenamiphos 0.19 U ug/L 0.19 0.7/21/2008 11:30 Efluent Pesticides Fonofos 0.096 U ug/L 0.096 0.7/21/2008 11:30 Efluent Pesticides Metolachlor 0.48 U ug/L 0.48 1.7/21/2008 11:30 Efluent Pesticides Metribuzin 0.096 U ug/L 0.096 0.7/21/2008 11:30 Efluent Pesticides Parathion Ethyl 0.14 U ug/L 0.14 0.7/21/2008 11:30 Efluent Pesticides Parathion Methyl 0.096 U ug/L 0.096 0.7/21/2008 11:30 Efluent Priority Organic Pollutants Benzo(a)pyrene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Indeno(1,2,3-cd)pyrene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Isophorone 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Phenol 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants 4-Chloro-3-methylphenol 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Benzo(b)fluoranthene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Bis(2-chloroisopropyl)ether 2.9 U ug/L 2.9 12 7/21/2008 11:30 Efluent Priority Organic Pollutants Diethyl phthalate 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Endrin aldehyde 3.8 U ug/L 3.8 15 7/21/2008 11:30 Efluent Priority Organic Pollutants Heptachlor 1.4 U ug/L 1.4 5.7/21/2008 11:30 Efluent Priority Organic Pollutants Phenanthrene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants 2-Nitrophenol 0.96 U ug/L 0.96 3.
24

Appendix E. (continued)

Date Sampled Field ID Analysis Component Result Remark Units MDL PQ

7/21/2008 11:30 Efluent Priority Organic Pollutants 4,4'-DDT 1.4 U ug/L 1.4 5.7/21/2008 11:30 Efluent Priority Organic Pollutants 4-Chlorophenyl phenyl ether 1.9 U ug/L 1.9 7.7/21/2008 11:30 Efluent Priority Organic Pollutants Benzo(g,h,i)perylene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Bis(2-chloroethoxy)methane 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Dibenzo(a,h)anthracene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Endosulfan II 3.8 U ug/L 3.8 15 7/21/2008 11:30 Efluent Priority Organic Pollutants Endrin 1.4 U ug/L 1.4 5.7/21/2008 11:30 Efluent Priority Organic Pollutants Naphthalene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Pentachlorophenol 2.9 U ug/L 2.9 12 7/21/2008 11:30 Efluent Priority Organic Pollutants 1,2,4-Trichlorobenzene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants 2,4,6-Trichlorophenol 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants 2,4-Dimethylphenol 9.6 U ug/L 9.6 38 7/21/2008 11:30 Efluent Priority Organic Pollutants 2-Chlorophenol 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants 2-Methyl-4,6-dinitrophenol 2.9 U ug/L 2.9 12 7/21/2008 11:30 Efluent Priority Organic Pollutants Aldrin 1.4 U ug/L 1.4 5.7/21/2008 11:30 Efluent Priority Organic Pollutants Dieldrin 1.4 U ug/L 1.4 5.7/21/2008 11:30 Efluent Priority Organic Pollutants Fluorene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Heptachlor epoxide 1.4 U ug/L 1.4 5.7/21/2008 11:30 Efluent Priority Organic Pollutants N-Nitrosodi-n-propylamine 1.9 U ug/L 1.9 7.7/21/2008 11:30 Efluent Priority Organic Pollutants Acenaphthylene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Benzo(a)anthracene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Benzo(k)fluoranthene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Bis(2-chloroethyl)ether 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Chrysene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Hexachlorocyclopentadiene 2.9 U ug/L 2.9 12 7/21/2008 11:30 Efluent Priority Organic Pollutants Hexachloroethane 2.9 U ug/L 2.9 12 7/21/2008 11:30 Efluent Priority Organic Pollutants beta-BHC 1.4 U ug/L 1.4 5.7/21/2008 11:30 Efluent Priority Organic Pollutants Anthracene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Benzidine 96 U ug/L 96 387/21/2008 11:30 Efluent Priority Organic Pollutants Bis(2-ethylhexyl)phthalate 14 U ug/L 14 58 7/21/2008 11:30 Efluent Priority Organic Pollutants Di-n-butyl phthalate 4.8 U ug/L 4.8 19 7/21/2008 11:30 Efluent Priority Organic Pollutants Di-n-octyl phthalate 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Hexachlorobenzene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Hexachlorobutadiene 2.9 U ug/L 2.9 12 
25

Appendix E. (continued)

Date Sampled Field ID Analysis Component Result Remark Units MDL PQ

7/21/2008 11:30 Efluent Priority Organic Pollutants Nitrobenzene 1.9 U ug/L 1.9 7.7/21/2008 11:30 Efluent Priority Organic Pollutants Pyrene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants 2,4-Dinitrotoluene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants 4-Bromophenyl phenyl ether 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Dimethyl phthalate 9.6 UJ ug/L 9.6 38 7/21/2008 11:30 Efluent Priority Organic Pollutants Endosulfan sulfate 1.4 U ug/L 1.4 5.7/21/2008 11:30 Efluent Priority Organic Pollutants alpha-BHC 1.4 U ug/L 1.4 5.7/21/2008 11:30 Efluent Priority Organic Pollutants delta-BHC 1.4 U ug/L 1.4 5.7/21/2008 11:30 Efluent Priority Organic Pollutants gamma-BHC 1.4 U ug/L 1.4 5.7/21/2008 11:30 Efluent Priority Organic Pollutants 2,4-Dichlorophenol 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants 4-Nitrophenol 14 U ug/L 14 58 7/21/2008 11:30 Efluent Priority Organic Pollutants Acenaphthene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants Butyl benzyl phthalate 4.8 U ug/L 4.8 19 7/21/2008 11:30 Efluent Priority Organic Pollutants Endosulfan I 3.8 U ug/L 3.8 15 7/21/2008 11:30 Efluent Priority Organic Pollutants Fluoranthene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants N-Nitrosodimethylamine 1.9 U ug/L 1.9 7.7/21/2008 11:30 Efluent Priority Organic Pollutants 2,4-Dinitrophenol 14 U ug/L 14 58 7/21/2008 11:30 Efluent Priority Organic Pollutants 2,6-Dinitrotoluene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants 2-Chloronaphthalene 0.96 U ug/L 0.96 3.7/21/2008 11:30 Efluent Priority Organic Pollutants 3,3'-Dichlorobenzidine 38 U ug/L 38 157/21/2008 11:30 Efluent Priority Organic Pollutants 4,4'-DDD 1.4 U ug/L 1.4 5.7/21/2008 11:30 Efluent Priority Organic Pollutants 4,4'-DDE 1.4 U ug/L 1.4 5.7/21/2008 11:30 Efluent Field Parameter Sample Depth 1 m 7/21/2008 11:30 Efluent Field Parameter pH 7.52 7/21/2008 11:30 Efluent Field Parameter Specific Conductance 0.34 umhos/cm 7/21/2008 11:30 Efluent Field Parameter Temperature 35.74 C 7/21/2008 11:30 Efluent Field Parameter Dissolved Oxygen 4.9 mg/L 
26

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 1 4 9 0 11 12 0 8 0 7 2 1 18 B 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 1 4 9 0 11 12 0 8 0 7 2 1 18 X 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 1 4 9 2 11 12 0 8 0 7 2 1 18 S 19 S 20 2

66

Biological Analyses of Florida Power and Light-Fort Myers Plant 

effluent sampled on July 21, 2008. NPDES #FL0001490.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

The Bioassay of Florida Power and Light-Fort Myers Plant effluent 

sampled on July 21, 2008. NPDES #FL0001490.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

The Priority Pollutants Analysis of Florida Power and Light-Fort 

Myers Plant effluent sampled on July 21, 2008. NPDES 

#FL0001490.