| (Mark One) |
|------------|
|       |   |
| x          | Annual Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 |
|       | For the fiscal year ended December 31, 2010 or |
| ¨     | Transition Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 |
|       | For the transition period from ___________________ to _______________________ |

| Massachusetts                                                  |   | 04-2652826                           |
|----------------------------------------------------------------|--------|--------------------------------------|
| (State or Other Jurisdiction of Incorporation or Organization) |   | (I.R.S. Employer Identification No.) |
| 14 Norfolk AvenueSouth Easton, Massachusetts                   |   | 02375                                |
| (Address of Principal Executive Offices)                       |   | ( Zip  Code)               |
| (508) 230-1828                                                 |   |                                 |
| (Registrant’s Telephone Number, Including Area Code)     |   |                                 |

| Securities registered pursuant to Section 12(b) of the Act:           |
|-----------------------------------------------------------------------|
| Title of Each Class                                              | Name of Each Exchange on Which Registered  |
| Common Stock, par value $.01 per sharePreferred Share Purchase Rights | The Nasdaq Stock Market, LLC |
|                                                                  |
| Securities registered pursuant to Section 12(g) of the Act:           |
|                                                                  |
| (Title of Class)                                                      |

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| ITEM 10. | DIRECTORS, EXECUTIVE OFFICERS AND CORPORATE GOVERNANCE. |
|----------|---------------------------------------------------------|

| Name                         |   | Age |   | Position                                                           |   | DirectorSince |   | Year Term Expiresand Class |   |
|------------------------------|--------|-----|--------|--------------------------------------------------------------------|--------|---------------|--------|----------------------------|--------|
| R. Wayne Fritzsche (1)       |   | 62  |   | Chairman of the Board                                              |   | 2003          |   | 2012Class I                |   |
| Calvin A. Saravis, Ph.D. (2) |   | 81  |   | Director                                                           |   | 1986          |   | 2012Class I                |   |
| J. Donald Payne (3)          |   | 55  |   | Director                                                           |   | 2003          |   | 2013Class II               |   |
| Alan D. Rosenson (3)         |   | 46  |   | Director                                                           |   | 2009          |   | 2013Class II               |   |
| Richard T. Schumacher        |   | 60  |   | Director, President, Chief Executive Officer, Treasurer, and Clerk |   | 1978          |   | 2011Class III              |   |
| Alan I. Goldberg (4)         |   | 68  |   | Director                                                           |   | 2010          |   | 2011Class III              |   |
| Gregory G. Freitag(4)        |   | 49  |   | Director                                                           |   | 2010          |   | 2011Class III              |   |

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| ITEM 11. | EXECUTIVE COMPENSATION. |
|----------|-------------------------|

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|                                    |       |   |      |    |   |            |   |   |                     |   |   |   |   |
|-----------------------------------------|------------|--------|-----------|---------|--------|-----------------|--------|--------|--------------------------|--------|--------|--------|--------|
| Name and Principal Position             | FiscalYear |   | Salary(1) |    |   | OptionAwards(2) |   |   | All otherCompensation(3) |   |   | Total  |   |
|                                    |       |   |      |    |   |            |   |   |                     |   |   |   |   |
| Richard T. Schumacher                   | 2010       |   | $         | 281,456 |   |            | $      | -      |                     |   | $      | 26,640 |   |   | $ | 308,096 |   |
| President & Chief Executive Officer | 2009       |   |      | 279,594 |   |            |   | 88,517 |                     |   |   | 18,720 |   |   |   | 386,831 |   |
|                                    |       |   |      |    |   |            |   |   |                     |   |   |   |   |   |   |   |   |
| Edmund Ting, Ph.D                       | 2010       |   |      | 192,546 |   |            |   | -      |                     |   |   | 1,329  |   |   |   | 193,875 |   |
| Senior Vice President of Engineering    | 2009       |   |      | 194,940 |   |            |   | 55,375 |                     |   |   | 1,319  |   |   |   | 251,634 |   |
|                                    |       |   |      |    |   |            |   |   |                     |   |   |   |   |   |   |   |   |
| Matthew B. Potter                       | 2010       |   |      | 160,793 |   |            |   | -      |                     |   |   | 7,572  |   |   |   | 168,365 |   |
| Vice President of Sales                 | 2009       |   |      | 162,779 |   |            |   | 35,522 |                     |   |   | 1,791  |   |   |   | 200,092 |   |

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|                                    |   | Option Awards                                                     |   |   |                                                                    |   |   |   |   |   |
|-----------------------------------------|--------|-------------------------------------------------------------------|--------|--------|-------------------------------------------------------------------------|--------|--------|--------|--------|--------|
| Name                                    |   | Number of Securities Underlying Unexercised Options(#)Exercisable |   |   | Number of Securities Underlying Unexercised Options(#)Unexercisable (1) |   |   |   |   |   | Option Exercise Price($) |   | Option Expiration Date |
|                                    |   |                                                              |   |   |                                                                    |   |   |   |   |   |   |   |   |
| Richard T. Schumacher                   |   |                                                              | 40,000 |   |                                                                    |   | 0      |   |   |   |   |   | $ | 2.60 |   | 5/2/2011 |
| President & Chief Executive Officer |   |                                                              | 60,000 |   |                                                                    |   | 0      |   |   |   |   |   | $ | 3.08 |   | 2/11/2012 |
|                                    |   |                                                              | 30,000 |   |                                                                    |   | 0      |   |   |   |   |   | $ | 2.70 |   | 12/2/2012 |
|                                    |   |                                                              | 75,000 |   |                                                                    |   | 0      |   |   |   |   |   | $ | 2.92 |   | 6/17/2015 |
|                                    |   |                                                              | 30,000 |   |                                                                    |   | 0      |   |   |   |   |   | $ | 3.86 |   | 3/30/2016 |
|                                    |   |                                                              | 70,000 |   |                                                                    |   | 0      |   |   |   |   |   | $ | 3.51 |   | 2/12/2017 |
|                                    |   |                                                              | 50,000 |   |                                                                    |   | 25,000 |   |   |   |   | (2) |   | $ | 0.77 |   | 3/12/2019 |
|                                    |   |                                                              |   |   |                                                                    |   |   |   |   |   |   |   |   |   |   |   |   |
| Edmund Y. Ting, Ph.D                    |   |                                                              | 60,000 |   |                                                                    |   | 0      |   |   |   |   |   |   | $ | 3.87 |   | 4/24/2016 |
| Senior Vice President of Engineering    |   |                                                              | 8,000  |   |                                                                    |   | 4,000  |   |   |   |   | (3) |   | $ | 2.75 |   | 9/25/2018 |
|                                    |   |                                                              | 28,000 |   |                                                                    |   | 14,000 |   |   |   |   | (3) |   | $ | 0.77 |   | 3/12/2019 |
|                                    |   |                                                              |   |   |                                                                    |   |   |   |   |   |   |   |   |   |   |   |   |
| Matthew B. Potter                       |   |                                                              | 23,333 |   |                                                                    |   | 11,667 |   |   |   |   | (4) |   | $ | 4.18 |   | 2/25/2018 |
| Vice President of Sales                 |   |                                                              | 3,333  |   |                                                                    |   | 1,667  |   |   |   |   | (4) |   | $ | 2.75 |   | 9/25/2018 |
|                                    |   |                                                              | 23,334 |   |                                                                    |   | 11,666 |   |   |   |   | (4) |   | $ | 0.77 |   | 3/12/2019 |

|   | (1) | All unvested stock options listed in this column were granted to the Named Executive Officer pursuant to the Company’s 2005 Equity Incentive Plan. All of such stock options vest ratably over three years in equal yearly installments except the stock options granted on March 12, 2009 of which one-third vest immediately and two-thirds vest over two years in equal yearly installments.  All options expire ten years after the date of grant.  Unvested stock options become fully vested and exercisable upon a change of control of the Company. |
|--------|-----|---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

|   | (2) | Options to purchase 75,000 shares of Common Stock were granted to Mr. Schumacher on March 12, 2009, of which 25,000 shares became immediately vested on March 12, 2009 and 25,000 became vested on March 12, 2010.  The remaining 25,000 became vested on March 12, 2011. |
|--------|-----|-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

|   | (3) | Options to purchase 12,000 shares of Common Stock were granted to Dr. Ting on September 25, 2008, of which 4,000 shares became vested on September 25, 2009 and 4,000 shares became vested on September 25, 2010.  The remaining 4,000 shares will become vested on September 25, 2011.  Options to purchase 42,000 shares of Common Stock were granted to Dr. Ting on March 12, 2009, of which 14,000 shares became immediately vested on March 12, 2009 and 14,000 shares became vested on March 12, 2010.  The remaining 14,000 became vested on March 12, 2011. |
|--------|-----|---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

|   | (4) | Options to purchase 35,000 shares of Common Stock were granted to Mr. Potter on February 25, 2008, of which 11,666 shares became vested on February 25, 2009 and 11,667 became vested on February 25, 2009.  The remaining 11,667 shares became vested on February 25, 2011.  Options to purchase 5,000 shares of Common Stock were granted to Mr. Potter on September 25, 2008, of which 1,666 shares became vested on September 25, 2009 and 1,667 became vested on September 25, 2010.  The remaining 1,667 will become vested on September 25, 2011. Options to purchase 35,000 shares of Common Stock were granted to Mr. Potter on March 12, 2009, of which 11,668 shares became immediately vested on March 12, 2009 and 11,666 became vested on March 12, 2010.  The remaining 11,666 became vested on March 12, 2011. |
|--------|-----|-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

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| Name                    |   | Fees Earned or Paid in Cash (1) |   |   | Option Awards (2)(3) |   |   | Total  |   |
|-------------------------|--------|---------------------------------|--------|--------|----------------------|--------|--------|--------|--------|
|                    |   |                            |   |   |                 |   |   |   |   |
| R. Wayne Fritzsche      |   | $                               | 10,000 |   |                 | $      | -      |   |   | $ | 10,000 |   |
| Calvin A. Saravis, Ph.D |   |                            | 10,000 |   |                 |   | -      |   |   |   | 10,000 |   |
| J. Donald Payne         |   |                            | 10,000 |   |                 |   | -      |   |   |   | 10,000 |   |
| Alan D. Rosenson        |   |                            | 10,000 |   |                 |   | -      |   |   |   | 10,000 |   |
| Alan I. Goldberg        |   |                            | 5,000  |   |                 |   | 26,663 |   |   |   | 31,663 |   |
| Gregory G. Freitag      |   |                            | 5,000  |   |                 |   | 26,663 |   |   |   | 31,663 |   |

| Name                    | Aggregate Number of Stock Options Outstanding |
|-------------------------|-----------------------------------------------|
|                    |                                          |
| R. Wayne Fritzsche      |  138,000                                 |
| Calvin A. Saravis, Ph.D |  125,000                                 |
| J. Donald Payne         |  88,000                                  |
| Alan D. Rosenson        |  25,000                                  |
| Alan I. Goldberg        |  25,000                                  |
| Gregory G. Freitag      |  25,000                                  |

| ITEM 12. | SECURITY OWNERSHIP OF CERTAIN BENEFICIAL OWNERS AND MANAGEMENT AND RELATED STOCKHOLDER MATTERS. |
|----------|-------------------------------------------------------------------------------------------------|

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| Name                                                                         | Number of Shares of Common Stock Beneficially Owned(1) | Percent of Class |
|------------------------------------------------------------------------------|--------------------------------------------------------|------------------|
|                                                                         |                                                   |             |
| Richard T. Schumacher(2)                                                | 702,828                                                | 21.1%            |
| R. Wayne Fritzsche(3)                                                   | 172,252                                                | 5.8%             |
| Calvin A. Saravis, Ph.D(4)                                              | 110,000                                                | 3.7%             |
| Edmund Y. Ting, Ph.D(5)                                                 | 124,682                                                | 4.2%             |
| Matthew B. Potter(6)                                                    | 76,473                                                 | 2.6%             |
| J. Donald Payne(7)                                                      | 126,454                                                | 4.3%             |
| Alan D. Rosenson(8)                                                     | 127,226                                                | 4.3%             |
| Alan I. Goldberg(9)                                                     | 121,060                                                | 4.1%             |
| Gregory G. Freitag(10)                                                  | 69,423                                                 | 2.4%             |
|   All Executive Officers and Directors as a Group (eleven persons) | 1,853,588                                              | 42.2%            |

| (1)   | The terms of the Preferred Stock and warrants contain a limitation on conversion which prevents the holder from converting shares of Preferred Stock into, or exercise of the warrants for, shares of Common Stock if, after giving effect to the conversion or exercise, as the case may be, the holder would beneficially own more than 4.99% of the outstanding shares of Common Stock. The holder may elect to increase this limitation to 9.99%, 14.99% or 19.99%, upon not less than 61 days prior written notice to the Company. With respect to Mr. Schumacher, because he currently beneficially owns more than 19.99% of the outstanding shares of Common Stock, the conversion limitation no longer applies to him. |
|-----------------|--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

| (2)   | Includes (i) 380,000 shares of Common Stock issuable upon exercise of options; (ii) 33,900 shares of Common Stock issuable upon conversion of Series A Convertible Preferred Stock; (iii) 50,180 shares of Common Stock issuable upon conversion of Series C Convertible Preferred Stock; and (iv) 21,740 shares of Common Stock issuable upon the exercise of warrants.  Does not include 20,162 shares of Common Stock held by Mr. Schumacher’s minor son as his wife exercises all voting and investment control over such shares. |
|-----------------|-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

| (3)   | Includes 138,000 shares of Common Stock issuable upon exercise of options.  Excludes 510,240 shares of Common Stock issuable upon conversion of the Preferred Stock and exercise of warrants held by Mr. Fritzsche because such exercise is subject to “blocker” provisions as described above. |
|-----------------|-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

| (4)   | Includes 110,000 shares of Common Stock issuable upon exercise of options.  Options to purchase 15,000 shares of Common Stock expired on April 23, 2011. |
|-----------------|--------------------------------------------------------------------------------------------------------------------------------------------------------------------|

| (5)   | Includes (i) 110,000 shares of Common Stock issuable upon exercise of options; (ii) 6,820 shares of Common Stock issuable upon conversion of Series A Convertible Preferred Stock; and (iii) 5,220 shares of Common Stock issuable upon the exercise of warrants. |
|-----------------|------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

| (6)   | Includes (i) 73,333 shares of Common Stock issuable upon exercise of options; (ii) 1,680 shares of Common Stock issuable upon conversion of Series A Convertible Preferred Stock; and (iii) 1,300 shares of Common Stock issuable upon the exercise of warrants. |
|-----------------|------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

| (7)   | Includes (i) 88,000 shares of Common Stock issuable upon exercise of options; (ii) 16,230 shares of Common Stock issuable upon conversion of Series A Convertible Preferred Stock; and (iii) 13,050 shares of Common Stock issuable upon the exercise of warrants. |
|-----------------|--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

| (8)   | Includes (i) 25,000 shares of Common Stock issuable upon exercise of options; and (ii) 67,850 shares of Common Stock issuable upon conversion of Series A Convertible Preferred Stock.  Excludes 131,500 shares of Common Stock issuable upon exercise of warrants held by Mr. Rosenson because such exercise is subject to “blocker” provisions as described above. |
|-----------------|--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

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| (9)   | Includes (i) 15,000 shares of Common Stock issuable upon exercise of options; and (ii) 96,960 shares of Common Stock issuable upon conversion of Series A Convertible Preferred Stock.  Options to purchase 10,000 shares of Common Stock will fully vest on July 27, 2011.  Excludes 96,960 shares of Common Stock issuable upon the exercise of warrants held by Mr. Goldberg because such exercise is subject to “blocker” provisions as described above. |
|-----------------|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

| (10)   | Includes (i) 15,000 shares of Common Stock issuable upon exercise of options; (ii) 26,640 shares of Common Stock issuable upon conversion of Series B Convertible Preferred Stock; and (iii) 26,640 shares of Common Stock issuable upon the exercise of warrants.  Options to purchase 10,000 shares of Common Stock will fully vest on July 27, 2011. |
|------------------|-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|

| Plan Category                                          |   | Number of securities to be issued upon exercise of outstanding options |      |   | Weighted-average exercise price of outstanding options |   |   | Number of securities remaining available for future issuance under equity compensation plans |   |
|--------------------------------------------------------|--------|------------------------------------------------------------------------|-----------|--------|--------------------------------------------------------|--------|--------|----------------------------------------------------------------------------------------------|--------|
| Equity compensation plans approved by security holders |   |                                                                   | 1,605,603 |   |                                                   | $      | 2.49   |                                                                                         |   |   | 433,397 |   |

|   | Includes the following plans: 1999 Non-Qualified Stock Option Plan and 2005 Equity Incentive Plan. |
|--------|----------------------------------------------------------------------------------------------------|

| ITEM 13. | CERTAIN RELATIONSHIPS AND RELATED TRANSACTIONS; AND DIRECTOR INDEPENDENCE. |
|----------|----------------------------------------------------------------------------|

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| Name and Position                                                   |   | Number of Units Purchased |   |   | Purchase Price |   |
|---------------------------------------------------------------------|--------|------------------------------------------|--------|--------|---------------------|--------|
| R. Wayne Fritzsche, Chairman of the Board                           |   |                                     | 21,931 |   |                | $      | 252,207 |   |
| J. Donald Payne, Director                                           |   |                                     | 1,305  |   |                | $      | 15,008 |   |
| Richard T. Schumacher, President and Chief Executive Officer        |   |                                     | 2,174  |   |                | $      | 25,001 |   |
| Nathan P. Lawrence, PhD., Vice President of Marketing               |   |                                     | 522    |   |                | $      | 6,003 |   |
| Alexander Lazarev, PhD., Vice President of Research and Development |   |                                     | 435    |   |                | $      | 5,003 |   |
| Matthew B. Potter, Vice President of Sales                          |   |                                     | 130    |   |                | $      | 1,495 |   |
| Edmund Y. Ting, PhD., Senior Vice President of Engineering          |   |                                     | 522    |   |                | $      | 6,003 |   |
| Alan I. Goldberg, Director                                          |   |                                     | 9,696  |   |                | $      | 111,504 |   |

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| ITEM 14. | PRINCIPAL ACCOUNTANT FEES AND SERVICES |
|----------|----------------------------------------|

|               |   | Fiscal 2010 Fees |    |   | Fiscal 2009 Fees |   |
|--------------------|--------|------------------|---------|--------|------------------|--------|
| Audit Fees         |   | $                | 111,696 |   |             | $      | 113,315 |   |
| Audit-Related Fees |   |             | 3,500   |   |             |   | 10,243 |   |
| Tax and Other Fees |   |             | -       |   |             |   | - |   |
|               |   | $                | 115,196 |   |             | $      | 123,558 |   |

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| PART IVITEM 15. |  EXHIBITS.                                                                                                                                                                          |           |
|-----------------|-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|----------------|
| Exhibit No.     |                                                                                                                                                                                          | Reference      |
| 31.1            | Principal Executive Officer and Principal Financial Officer Certification Pursuant to Item 601(b)(31) of Regulation S-K, as adopted pursuant to Section 302 of the Sarbanes-Oxley Act of 2002 | Filed herewith |
| 32.1            | Principal Executive Officer and Principal Financial Officer Certification Pursuant to Item 601(b)(32) of Regulation S-K, as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002 | Filed herewith |

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|                          |                                         |                      |
|-------------------------------|----------------------------------------------|---------------------------|
| Date:  May 2, 2011  | Pressure BioSciences, Inc.                   |
|                          |                                         |                      |
|                          | By:                                | /s/ Richard T. Schumacher |
|                          | Richard T. Schumacher                        |
|                          | President and Chief Executive Officer   |

| SIGNATURES                                                        |   | TITLES                                                                                                                         |   | DATE        |
|-------------------------------------------------------------------|--------|--------------------------------------------------------------------------------------------------------------------------------|--------|-------------|
|                                                              |   |                                                                                                                           |   |        |
| /s/Richard T. Schumacher  Richard T. Schumacher         |   | President, Chief Executive Officer (Principal Executive Officer, Principal Financial Officer and Principal Accounting Officer) |   | May 2, 2011 |
|                                                              |   |                                                                                                                           |   |        |
| /s/ R. Wayne Fritzsche  R. Wayne Fritzsche              |   | Director and Chairman of the Board                                                                                             |   | May 2, 2011 |
|                                                              |   |                                                                                                                           |   |        |
| /s/J. Donald Payne  J. Donald Payne                     |   | Director                                                                                                                       |   | May 2, 2011 |
|                                                              |   |                                                                                                                           |   |        |
| /s/ Calvin A. Saravis, Ph.D.  Calvin A. Saravis, Ph. D. |   | Director                                                                                                                       |   | May 2, 2011 |
|                                                              |   |                                                                                                                           |   |        |
| /s/ Alan D. Rosenson  Alan D. Rosenson                  |   | Director                                                                                                                       |   | May 2, 2011 |
|                                                              |   |                                                                                                                           |   |        |
| /s/ Alan I. Goldberg  Alan I. Goldberg                  |   | Director                                                                                                                       |   | May 2, 2011 |
|                                                              |   |                                                                                                                           |   |        |
| /s/ Gregory G. Freitag  Gregory G. Freitag              |   | Director                                                                                                                       |   | May 2, 2011 |