Effluent Quality Assessment 

Tampa Electric Company Big Bend Power Station 

Hillsborough County 

NPDES #FL0000817 

Sampled June 15, 2009 

September 2009

Biology Section 

Bureau of Laboratories 

Division of Environmental Assessment and Restoration 

Quality Manual No. 870346G 

NELAC Certification No. E31780
1 

CONTENTS

Introduction 

Methods 

Results and Discussion 

Literature Cited 

Tables 

Table 1 Measured algal growth potential (AGP) 

Table 2 Effluent limits, Class III Marine Criteria and chemical data 

Appendices 

Appendix A Chain of Custody 

Appendix B Bioassay Bench Sheets 

Appendix C SRT Bench Sheets and Statistical Analyses 

Appendix D Organism Receipt 

Appendix E Chemical Analyses of Effluent 

Appendix F Facility Summary, Effluent Limits, and Permit Violations 

Recorded on Discharge Monitoring Report 

Appendix G EPA End Sheet 
2 

Tampa Electric Company Big Bend Power Station, 13031 Wyandotte Road, Apollo Beach, 

Hillsborough County, Florida, 33572. NPDES #FL0000817. Effluent grab samples for bioassay 

and chemical analyses for this facility were collected on June 15, 2009. 

Introduction 

Tampa Electric Company Big Bend Power Station is an electric power generating facility which 

consists of four coal-fired steam electric units with a total nameplate rating of 1,823 MegaWatts 

(MW) and three oil-fired combustion turbines with a combined capacity of 175 MW. The facility is 

currently operating under a Consent Order (OGC File # 98-2888D) concerning depressed 

dissolved oxygen concentrations in the effluent as compared to DO concentrations in the influent. 

(See Appendix F for detailed facility information provided by Pete Wenner of FDEP Southwest 

District.) 

Methods 

Samples were collected from the EFF-1 NPDES sampling point for Outfall D-001 by Jason Cohen 

and Peter Wenner (FDEP Southwest District) following DEP-SOP-001/01 FS 2400 Wastewater 

Sampling. All DEP SOPs are available on the web at: http://www.floridadep.org/labs/qa/sops.htm. 

See Appendix A for the completed chain of custody form. 

The 48-hour acute screening toxicity tests and algal growth potential test were performed 

following internal DEP SOPs TA07_03, TA07_04, and TA08_01 through TA08_09. All internal 

DEP SOPs are available on the web at: http://www.floridadep.org/labs/cgi-bin/sop/biosop.asp. 

Results and Discussion 

Toxicity Test Results 

Tests were performed June 16 - 18, 2009. 

Americamysis bahia 48-hour acute screening bioassay – LC50 > 100%, 0%* mortality in 

 100% sample at 48 hours 

Menidia beryllina 48-hour acute screening bioassay – LC50 > 100%, 10%* mortality in 

 100% sample at 48 hours 

The bioassay samples were not acutely toxic to the test organisms. 

*NOTE: In a 48-hour acute screening test of 100% effluent, mortality of <20% provides 

confidence that the effluent meets the acute toxicity criterion of Florida’s Surface Water 

Quality Standards (Rule 62-302.500 (1) (a) 4, F.A.C.). Mortality between 20-50% indicates 

low to moderate levels of toxicity, and further action may be required. Mortality of >50% 

indicates that the effluent fails to meet the minimum requirement to discharge to waters of the 

state (Rule 62-4.244(3) (a), F.A.C.). 

See Appendix B for bioassay bench sheets. See Appendix C for results of the last 20 

standard reference toxicant (SRT) tests and the bench sheets and statistical analyses of the 

SRT tests that correspond to these facility tests. See Appendix D for test organism receipts. 

Algal Growth Potential 

The effluent Algal Growth Potential (AGP) was 3.0 U (U - Material was analyzed for but not 

detected; the value reported is the minimum detection limit) mg dry wt/L of the saltwater 
3 

species Dunaliella tertiolecta. Raschke and Schultz found that AGP values above 10.0 mg 

dry weight/L represent a “problem” threshold for marine receiving waters, implying nutrient 

enrichment (personal communication, Marshall Faircloth, et. al. FDEP, with Ron Raschke 

USEPA Region 4 1987). The ratio of total nitrogen to total phosphorus suggests that the 

effluent is nitrogen limited (Maloney et al. 1978). 

See Table 1 for AGP results. 

Chemistry Results 

Total residual chlorine and total ammonia were not detected in the bioassay sample in the 

laboratory. The total ammonia concentration in the composite sample collected and preserved 

for chemical analysis was 0.054 mg N/L. Based on the pH, salinity, and temperature of the 

effluent as collected, the calculated unionized ammonia concentration was < 0.02 mg/L. 

Total alpha particles and Combined Radium 226 + 228 were detected in the effluent at levels 

that comply with Class III marine water quality criteria (62-302.530, F.A.C.). Arsenic, chromium, 

iron, and nickel were detected between the laboratory method detection limits (MDL) and 

practical quantitation limits (PQL). Ortho-phosphate was detected in the field blank. 

See Table 2 for results of analytes detected in the effluent, and corresponding limits. See 

Appendix E for a complete list of chemical analyses performed. 

Conclusion 

The effluent sample collected from the EFF-1 for this facility on June 15, 2009, was not acutely 

toxic to either test species during the 48-hour acute screening bioassays. The effluent AGP result 

was less than the "problem” threshold for marine receiving waters. Effluent water quality samples 

collected on June 15, 2009, did not exceed any applicable water quality criteria or violate any 

permit conditions. 

Literature Cited 

Maloney, T. E., W. E. Miller, and D. T. Specht. 1974. The Marine Algal Assay Procedure: Bottle 

Test. National Environmental Research Center Office of Research and Development. U. S. 

EPA, Corvallis, Oregon. 43 p. 
4 

Inorganic Total

Location N:P ratio N:P ratio

D-001 3.0 U 0.4 4.7

Table 1. Measured algal growth potential (AGP; mg dry 

weight/L) of the saltwater species Dunaliella tertiolecta 

and ratios of nitrogen to phosphorus for samples 

collected from TECO Big Bend Power Station on June 15, 

2009.

AGP

(measured)

U - Material analyzed for but not detected; value reported is 

the method detection limit

Tampa Electric Company Big Bend Power Station 

NPDES # FL0000817
5 

Tampa Electric Company Big Bend Power 

Station NPDES# 

FL0000817

Class III 

Criteria

Effluent 

Limits

Aluminum ≤ 1,500 - 180 U

Antimony ≤ 4,300 - 1 U

Arsenic ≤ 50 - 1.8 I

Beryllium ≤ 0.13 a - 0.075 U

Cadmium ≤ 8.8 - 0.12 U

Calcium (mg/L) - - 363

Chromium-III - - 2.8 I

Copper ≤ 3.7 - 2 U

Iron ≤ 300 - 170 I

Lead ≤ 8.5 - 0.8 U

Magnesium (mg/L) - - 1110

Nickel ≤ 8.3 - 1 I

Selenium ≤ 71 - 0.8 U

Silver ≤ 2.3 - 0.1 U

Zinc ≤ 86 - 15 U

Ortho-phosphate - - 0.13 F

Total Phosphorus - - 0.18

Total Ammonia - - 0.054

Un-ionized Ammonia - - 0.01 c

Nitrate and Nitrite - - 0.004 U

Total Kjeldahl Nitrogen - - 0.84

Organic Nitrogen - - 0.786 c

Total Nitrogen - - 0.842 c

Dissolved Oxygen (mg/L) ≥ 4.0 ≥ 4.0 i 4.3

pH (SU) 6.5 - 8.5 6.5 - 8.5 7.9

Conductivity (μmhos/cm) - - 47,000

Temperature (°C) - 42.78 i 38.5

Salinity - - 30.4 £

Sample Depth (m) - - 0.2

Total Residual Chlorine (mg/L) ≤ 0.01 - ≤ 0.03 £

Chlorophyll a (μg/L) - Corrected -- 3

Phaeophytin (μg/L) - - 1.3

Total Suspended Solids (mg/L) - - 7 I

CBOD, 5 day (mg/L), N - inhibited - - 1.4 I

Oils and Greases (mg/L) ≤ 5.0 - 1.4 U

Alpha, Total (pCi/L) ≤ 15 - 2.1

Alpha-Counting Error (pCi/L) - - 1.5

Radium 226 (pCi/L) - - 1

Radium 226-Counting Error (pCi/L) - - 0.2

Radium 228 (pCi/L) - - 0.9 U

Radium 228-Counting Error (pCi/L) - - 0.6

Radium 226 + 228 (pCi/L) ≤ 5 - 1c

Flow (MGD) - Report 1121.01 a

Hardness (mg CaCO3) - - 5477.39 c

Value exceeds the Class III Water Quality Criteria or permit limits

a - Annual average

c - Value is calculated

i - Instantateous Maximum

F - Analyte was detected in both sample and field blank

I - The reported value is between the laboratory method detection limit and the laboratory practical quantitation limit

U - Material analyzed for but not detected; value reported is the method detection limit

£ - Measured in Central labs and entered into Tox sheets

Table 2. Effluent limits, Class III Criteria for predominantly marine waters and chemical 

data for samples collected from the TECO Big Bend Power Station on June 15, 2009.

Nutrients (mg/L)

General Physical and Chemical Parameters

Effluent

Metals (μg/L unless otherwise noted)
6 

Appendix A. Chain of Custody Form 
7 

Appendix B. Bench Sheets 
8 

Appendix B. (continued) 
9 

Appendix B. (continued) 
10 

Appendix B. (continued) 
11 

Appendix B. (continued) 
12 

Appendix B. (continued) 
13 

Appendix C. SRT Bench Sheets and Statistical Analysis 

Florida Department of Environmental Protection

Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - 48-hour Acute Toxicity

Americamysis bahia (mysid shrimp) - Cadmium Chloride (CdCl2) 

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit

2/5/2008 * 23.52 50.68 13.02 24.64 76.72

02/12/08 36.63 49.45 13.14 23.17 75.74

03/11/08 38.21 47.42 11.38 24.66 70.19

04/01/08 53.27 46.90 10.82 25.26 68.53

05/06/08 35.65 45.82 10.81 24.19 67.45

06/03/08 58.28 46.36 11.17 24.03 68.69

07/08/08 47.67 46.00 10.99 24.02 67.98

08/05/08 44.68 46.32 10.84 24.63 68.01

09/03/08 45.94 45.82 10.60 24.62 67.02

10/07/08 41.43 44.39 8.96 26.47 62.30

11/04/08 46.75 44.58 8.97 26.64 62.51

11/12/09 44.92 44.87 8.87 27.13 62.61

12/02/08 40.59 44.42 8.84 26.73 62.11

01/06/09 37.22 43.80 8.89 26.01 61.59

02/03/09 39.02 43.57 8.96 25.66 61.49

03/03/09 30.58 42.81 9.40 24.02 61.61

03/31/09 38.24 42.65 9.45 23.76 61.55

04/08/09 31.38 42.44 9.66 23.12 61.76

05/05/09 33.86 41.62 9.66 22.31 60.93

06/09/09 41.42 40.46 8.00 24.47 56.45

Lower Confidence Limit = mean - 2 * Std Dev CV = 19.76

Upper Confidence Limit = mean + 2 * Std Dev EPA reference 75th quartile CV = 26%

Standard Reference Toxicant Data 

Americamysis bahia 48-hour LC50

20

30

40

50

60

70

80

2/5/2008 *

02/12/0803/11/0804/01/0805/06/0806/03/0807/08/0808/05/0809/03/0810/07/0811/04/0811/12/0912/02/0801/06/0902/03/0903/03/0903/31/0904/08/0905/05/0906/09/09

Test Date

μg/L CdCl2

LC50 Mean LC50 Lower Control Limit Upper Control Limit
14 

Appendix C. (continued) 

Florida Department of Environmental Protection

Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - Acute Toxicity

Menidia beryllina (Inland silverside) - Copper Chloride (CuCl2)

Date LC50 Mean LC50 Std Dev

Lower 

Control Limit

Upper 

Control Limit

01/08/08 329.03 285.29 71.1 143.0 427.5

02/05/08 357.47 294.07 68.5 157.1 431.0

03/04/08 295.52 295.31 68.1 159.3 431.9

04/01/08 379.50 304.05 67.5 169.0 439.1

05/06/08 272.50 304.73 67.1 170.5 438.9

06/03/08 373.47 312.52 65.5 181.5 443.5

07/08/08 275.20 316.13 61.0 194.2 438.1

08/12/08 221.17 320.17 50.5 219.2 421.1

09/03/08 167.46 313.48 60.9 191.7 435.3

09/09/08 240.07 311.58 62.6 186.3 436.9

10/07/08 325.55 312.05 62.7 186.6 437.5

11/04/08 249.81 307.63 63.9 179.9 435.4

12/02/08 316.79 310.59 62.8 185.0 436.2

01/06/09 257.04 311.08 62.3 186.5 435.7

02/03/09 245.51 305.47 62.9 179.6 431.4

03/03/09 433.98 309.64 68.6 172.5 446.8

03/31/09 228.94 304.17 70.5 163.1 445.2

05/05/09 310.12 302.74 70.1 162.6 442.9

06/02/09 471.25 305.32 75.4 154.6 456.0

06/09/09 370.27 306.03 75.9 154.2 457.9

Means are of the last 20 SRT tests.

EPA reference 75th quartile CV = 21% CV = 24.81

Standard Reference Toxicant Data 

Menidia beryllina 48 hour LC50

0

50

100

150

200

250

300

350

400

450

500

01/08/0802/05/0803/04/0804/01/0805/06/0806/03/0807/08/0808/12/0809/03/0809/09/0810/07/0811/04/0812/02/0801/06/0902/03/0903/03/0903/31/0905/05/0906/02/0906/09/09

Test Date

μg/L CuCl2

Lower Control Limit LC50 Mean LC50 Upper Control Limit
15 

Appendix C. (continued) 
16 

Appendix C. (continued) 
17 

Appendix C. (continued) 
18 

Appendix C. (continued) 
19 

Appendix C. (continued) 

Americamysis bahia Acute Standard Reference Toxicant Test 

DATE: 6-9-09 DURATION: 48 hr TOXICANT: CdCl2 

 SPECIES: Americamysis bahia

 RAW DATA: Concentration Number Mortalities 

 (ug/L) Exposed 

 0.00 20 0 

 20.00 20 0 

 30.00 20 2 

 45.00 20 14 

 67.00 20 18 

 100.00 20 20 

 SPEARMAN-KARBER TRIM: .00% 

 SPEARMAN-KARBER ESTIMATES: LC50: 41.42 

 95% LOWER CONFIDENCE: 37.02 

 95% UPPER CONFIDENCE: 46.35 

Menidia beryllina Acute Standard Reference Toxicant Test 

DATE: 6-9-09 DURATION: 48 hr TOXICANT: CuCl2 

SPECIES: Menidia beryllina 

RAW DATA: Concentration Number Mortalities 

 (ug/l) Exposed 

 0.00 20 0 

 100.00 20 0 

 200.00 20 0 

 300.00 20 5 

 400.00 20 8 

 500.00 20 20 

 SPEARMAN-KARBER TRIM: .00% 

 SPEARMAN-KARBER ESTIMATES: LC50: 370.27 

 95% LOWER CONFIDENCE: 339.29 

 95% UPPER CONFIDENCE: 404.08 
20 

Appendix D. Test Organism Receipts 
21 

Appendix D. (continued) 
22 

Appendix E. Chemical Analyses performed on the effluent from Tampa Electric Company Big B

Outfall D-001, sampled on June 15, 2009.

Date Sampled Field ID Analysis Group Components Result Remarks Units 

6/15/2009 10:30 37693 AGP/LN Algal Growth Potential 3 U mg Dr6/15/2009 10:30 37693 Chlorophyll/Grain Size/BOD Chlorophyll-a, Corrected 3 ug/L 6/15/2009 10:30 37693 Chlorophyll/Grain Size/BOD Biochemical Oxygen Demand-5 Day,N-Inhib 1.4 I mg/L 6/15/2009 10:30 37693 Chlorophyll/Grain Size/BOD Phaeophytin-a 1.3 ug/L 6/15/2009 10:30 37693 Toxicology Bioassay-Acute-Screen-SW-Fish, LC50 100 L LC50 6/15/2009 10:30 37693 Toxicology Bioassay-Acute-Screen-SW-Mysid, LC50 100 L LC50 6/15/2009 10:30 37693 Metals Arsenic 1.8 I ug/L 6/15/2009 10:30 37693 Metals Zinc 15 U ug/L 6/15/2009 10:30 37693 Metals Beryllium 0.075 U ug/L 6/15/2009 10:30 37693 Metals Antimony 1 U ug/L 6/15/2009 10:30 37693 Metals Aluminum 180 U ug/L 6/15/2009 10:30 37693 Metals Nickel 1 I ug/L 6/15/2009 10:30 37693 Metals Chromium 2.8 I ug/L 6/15/2009 10:30 37693 Metals Calcium 363 mg/L 6/15/2009 10:30 37693 Metals Silver 0.1 U ug/L 6/15/2009 10:30 37693 Metals Selenium 0.8 U ug/L 6/15/2009 10:30 37693 Metals Magnesium 1110 mg/L 6/15/2009 10:30 37693 Metals Iron 170 I ug/L 6/15/2009 10:30 37693 Metals Copper 2 U ug/L 6/15/2009 10:30 37693 Metals Cadmium 0.12 U ug/L 6/15/2009 10:30 37693 Metals Lead 0.8 U ug/L 6/15/2009 10:30 37693 Nutrients Ammonia-N 0.054 mg N/6/15/2009 10:30 37693 Nutrients Total-P 0.18 mg P/6/15/2009 10:30 37693 Nutrients NO2NO3-N 0.004 U mg N/6/15/2009 10:30 37693 Nutrients TSS 7 I mg/L 6/15/2009 10:30 37693 Nutrients Kjeldahl Nitrogen 0.84 mg N/6/15/2009 10:30 37693 Nutrients O-Phosphate-P 0.13 mg P/6/15/2009 10:30 37693 Overflow Radium 228-Counting Error 0.6 pCi/L 6/15/2009 10:30 37693 Overflow Oil and Grease 1.4 U mg/L 6/15/2009 10:30 37693 Overflow Radium 228 0.9 U pCi/L 6/15/2009 10:30 37693 Overflow Alpha, Total 2.1 pCi/L 6/15/2009 10:30 37693 Overflow Radium 226 1 pCi/L 
23 

Appendix E. (continued)

Date Sampled Field ID Analysis Group Components Result Remarks Units 

6/15/2009 10:30 37693 Overflow Alpha-Counting Error 1.5 pCi/L 6/15/2009 10:30 37693 Overflow Radium 226-Counting Error 0.2 pCi/L 6/15/2009 10:30 37693 Pesticides Endosulfan Sulfate 0.02 U ug/L 6/15/2009 10:30 37693 Pesticides Norflurazon 0.15 U ug/L 6/15/2009 10:30 37693 Pesticides Aldrin 0.01 U ug/L 6/15/2009 10:30 37693 Pesticides Delta-BHC 0.01 U ug/L 6/15/2009 10:30 37693 Pesticides Metalaxyl 0.26 U ug/L 6/15/2009 10:30 37693 Pesticides Mevinphos 0.2 U ug/L 6/15/2009 10:30 37693 Pesticides Parathion Ethyl 0.15 U ug/L 6/15/2009 10:30 37693 Pesticides Simazine 0.051 U ug/L 6/15/2009 10:30 37693 Pesticides Endosulfan I 0.02 U ug/L 6/15/2009 10:30 37693 Pesticides Toxaphene 0.77 U ug/L 6/15/2009 10:30 37693 Pesticides Bromacil 0.2 U ug/L 6/15/2009 10:30 37693 Pesticides Ethoprop 0.1 U ug/L 6/15/2009 10:30 37693 Pesticides Metolachlor 0.51 UJ ug/L 6/15/2009 10:30 37693 Pesticides Naled 0.82 U ug/L 6/15/2009 10:30 37693 Pesticides DDD-p,p' 0.02 U ug/L 6/15/2009 10:30 37693 Pesticides DDE-p,p' 0.02 U ug/L 6/15/2009 10:30 37693 Pesticides Dieldrin 0.02 U ug/L 6/15/2009 10:30 37693 Pesticides Endrin 0.051 U ug/L 6/15/2009 10:30 37693 Pesticides Atrazine 0.051 U ug/L 6/15/2009 10:30 37693 Pesticides Azinphos Methyl 0.2 UJ ug/L 6/15/2009 10:30 37693 Pesticides Chlorpyrifos Ethyl 0.051 U ug/L 6/15/2009 10:30 37693 Pesticides Ethion 0.051 U ug/L 6/15/2009 10:30 37693 Pesticides Alpha-BHC 0.01 U ug/L 6/15/2009 10:30 37693 Pesticides DDT-p,p' 0.031 U ug/L 6/15/2009 10:30 37693 Pesticides Endosulfan II 0.02 U ug/L 6/15/2009 10:30 37693 Pesticides Diazinon 0.051 U ug/L 6/15/2009 10:30 37693 Pesticides Fenamiphos 0.2 U ug/L 6/15/2009 10:30 37693 Pesticides Malathion 0.15 U ug/L 6/15/2009 10:30 37693 Pesticides Phorate 0.051 U ug/L 6/15/2009 10:30 37693 Pesticides Endrin Aldehyde 0.02 U ug/L 6/15/2009 10:30 37693 Pesticides Gamma-BHC 0.01 U ug/L 6/15/2009 10:30 37693 Pesticides Alachlor 0.61 UJ ug/L 
24 

Appendix E. (continued)

Date Sampled Field ID Analysis Group Components Result Remarks Units 

6/15/2009 10:30 37693 Pesticides Hexazinone 0.1 U ug/L 6/15/2009 10:30 37693 Pesticides Beta-BHC 0.01 U ug/L 6/15/2009 10:30 37693 Pesticides Heptachlor 0.01 U ug/L 6/15/2009 10:30 37693 Pesticides Heptachlor Epoxide 0.02 U ug/L 6/15/2009 10:30 37693 Pesticides Methoxychlor 0.051 U ug/L 6/15/2009 10:30 37693 Pesticides Ametryn 0.051 UJ ug/L 6/15/2009 10:30 37693 Pesticides Butylate 0.2 UJ ug/L 6/15/2009 10:30 37693 Pesticides Chlorpyrifos Methyl 0.1 U ug/L 6/15/2009 10:30 37693 Pesticides Fonofos 0.1 UJ ug/L 6/15/2009 10:30 37693 Pesticides Metribuzin 0.1 U ug/L 6/15/2009 10:30 37693 Pesticides Parathion Methyl 0.1 U ug/L 6/15/2009 10:30 37693 Pesticides Prometryn 0.15 UJ ug/L 6/15/2009 10:30 37693 Pesticides Chlordane 0.2 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Benzidine 94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Dibenzo(a,h)anthracene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Diethyl phthalate 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Hexachlorobutadiene 2.8 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Hexachloroethane 2.8 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants N-Nitrosodi-n-propylamine 1.9 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants N-Nitrosodiphenylamine/ Diphenylamine 2.8 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Pyrene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Hexachlorobenzene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Indeno(1,2,3-cd)pyrene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants N-Nitrosodimethylamine 1.9 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Nitrobenzene 1.9 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants 2-Methyl-4,6-dinitrophenol 2.8 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants 4-Chlorophenyl phenyl ether 1.9 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Di-n-octyl phthalate 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Phenanthrene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants 2-Chloronaphthalene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants 2-Chlorophenol 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants 2-Nitrophenol 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants 3,3'-Dichlorobenzidine 38 UJ ug/L 
25 

Appendix E. (continued)

Date Sampled Field ID Analysis Group Components Result Remarks Units 

6/15/2009 10:30 37693 Priority Organic Pollutants 4-Bromophenyl phenyl ether 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants 4-Nitrophenol 14 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Bis(2-ethylhexyl)phthalate 14 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Chrysene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Di-n-butyl phthalate 4.7 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Dimethyl phthalate 9.4 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Fluoranthene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Fluorene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Isophorone 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Naphthalene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants 2,4-Dichlorophenol 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants 4-Chloro-3-methylphenol 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Benzo(g,h,i)perylene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Anthracene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Benzo(k)fluoranthene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Butyl benzyl phthalate 4.7 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Pentachlorophenol 2.8 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants 2,4-Dinitrophenol 14 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Acenaphthene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Acenaphthylene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Benzo(a)anthracene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Benzo(b)fluoranthene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Bis(2-chloroethoxy)methane 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Phenol 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants 1,2,4-Trichlorobenzene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants 2,4,6-Trichlorophenol 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants 2,4-Dimethylphenol 9.4 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants 2,4-Dinitrotoluene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants 2,6-Dinitrotoluene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Benzo(a)pyrene 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Bis(2-chloroethyl)ether 0.94 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Bis(2-chloroisopropyl)ether 2.8 U ug/L 6/15/2009 10:30 37693 Priority Organic Pollutants Hexachlorocyclopentadiene 2.8 UJ ug/L 
26 

Appendix E. (continued)

Date Sampled Field ID Analysis Group Components Result Remarks Units 

6/15/2009 10:30 37693 Field Parameter Sample Depth 0.2 m 6/15/2009 10:30 37693 Field Parameter Temperature 38.5 C 6/15/2009 10:30 37693 Field Parameter Specific Conductance 47000 umhos6/15/2009 10:30 37693 Field Parameter Dissolved Oxygen 4.32 mg/L 6/15/2009 10:30 37693 Field Parameter pH 7.94 6/15/2009 12:20 Field Blank Chlorophyll/Grain Size/BOD Biochemical Oxygen Demand-5 Day,N-Inhib 0.2 U mg/L 6/15/2009 12:20 Field Blank Metals Aluminum 60 U ug/L 6/15/2009 12:20 Field Blank Metals Cadmium 0.03 U ug/L 6/15/2009 12:20 Field Blank Metals Chromium 0.3 U ug/L 6/15/2009 12:20 Field Blank Metals Silver 0.025 U ug/L 6/15/2009 12:20 Field Blank Metals Nickel 0.25 U ug/L 6/15/2009 12:20 Field Blank Metals Antimony 0.25 U ug/L 6/15/2009 12:20 Field Blank Metals Selenium 0.2 U ug/L 6/15/2009 12:20 Field Blank Metals Calcium 0.075 U mg/L 6/15/2009 12:20 Field Blank Metals Magnesium 0.04 U mg/L 6/15/2009 12:20 Field Blank Metals Zinc 5 U ug/L 6/15/2009 12:20 Field Blank Metals Arsenic 0.25 U ug/L 6/15/2009 12:20 Field Blank Metals Iron 30 U ug/L 6/15/2009 12:20 Field Blank Metals Lead 0.2 U ug/L 6/15/2009 12:20 Field Blank Metals Copper 0.5 U ug/L 6/15/2009 12:20 Field Blank Metals Beryllium 0.025 U ug/L 6/15/2009 12:20 Field Blank Nutrients O-Phosphate-P 0.006 I mg P/6/15/2009 12:20 Field Blank Nutrients Total-P 0.004 U mg P/6/15/2009 12:20 Field Blank Nutrients NO2NO3-N 0.004 U mg N/6/15/2009 12:20 Field Blank Nutrients Ammonia-N 0.01 U mg N/6/15/2009 12:20 Field Blank Nutrients Kjeldahl Nitrogen 0.08 U mg N/6/15/2009 12:20 Field Blank Overflow Radium 228 0.9 U pCi/L 6/15/2009 12:20 Field Blank Overflow Radium 226 0.2 U pCi/L 6/15/2009 12:20 Field Blank Overflow Radium 226-Counting Error 0.1 pCi/L 6/15/2009 12:20 Field Blank Overflow Oil and Grease 1.4 U mg/L 6/15/2009 12:20 Field Blank Overflow Radium 228-Counting Error 0.5 pCi/L 6/15/2009 12:20 Field Blank Overflow Alpha-Counting Error 0.5 pCi/L 6/15/2009 12:20 Field Blank Overflow Alpha, Total 0.9 U pCi/L 6/15/2009 12:20 Field Blank Pesticides Azinphos Methyl 0.19 UJ ug/L 
27 

Appendix E. (continued)

Date Sampled Field ID Analysis Group Components Result Remarks Units 

6/15/2009 12:20 Field Blank Pesticides Hexazinone 0.097 U ug/L 6/15/2009 12:20 Field Blank Pesticides Naled 0.78 U ug/L 6/15/2009 12:20 Field Blank Pesticides Prometryn 0.15 UJ ug/L 6/15/2009 12:20 Field Blank Pesticides Beta-BHC 0.0097 U ug/L 6/15/2009 12:20 Field Blank Pesticides Heptachlor 0.0097 U ug/L 6/15/2009 12:20 Field Blank Pesticides Butylate 0.19 UJ ug/L 6/15/2009 12:20 Field Blank Pesticides Chlorpyrifos Ethyl 0.048 U ug/L 6/15/2009 12:20 Field Blank Pesticides Mevinphos 0.19 U ug/L 6/15/2009 12:20 Field Blank Pesticides Parathion Ethyl 0.15 U ug/L 6/15/2009 12:20 Field Blank Pesticides Simazine 0.048 U ug/L 6/15/2009 12:20 Field Blank Pesticides Endosulfan I 0.019 U ug/L 6/15/2009 12:20 Field Blank Pesticides Diazinon 0.048 U ug/L 6/15/2009 12:20 Field Blank Pesticides Chlordane 0.19 U ug/L 6/15/2009 12:20 Field Blank Pesticides DDD-p,p' 0.019 U ug/L 6/15/2009 12:20 Field Blank Pesticides DDT-p,p' 0.029 U ug/L 6/15/2009 12:20 Field Blank Pesticides Toxaphene 0.73 U ug/L 6/15/2009 12:20 Field Blank Pesticides Chlorpyrifos Methyl 0.097 U ug/L 6/15/2009 12:20 Field Blank Pesticides Fenamiphos 0.19 U ug/L 6/15/2009 12:20 Field Blank Pesticides Fonofos 0.097 UJ ug/L 6/15/2009 12:20 Field Blank Pesticides Malathion 0.15 U ug/L 6/15/2009 12:20 Field Blank Pesticides Metribuzin 0.097 U ug/L 6/15/2009 12:20 Field Blank Pesticides Phorate 0.048 U ug/L 6/15/2009 12:20 Field Blank Pesticides Aldrin 0.0097 U ug/L 6/15/2009 12:20 Field Blank Pesticides DDE-p,p' 0.019 U ug/L 6/15/2009 12:20 Field Blank Pesticides Dieldrin 0.019 U ug/L 6/15/2009 12:20 Field Blank Pesticides Gamma-BHC 0.0097 U ug/L 6/15/2009 12:20 Field Blank Pesticides Methoxychlor 0.048 U ug/L 6/15/2009 12:20 Field Blank Pesticides Ethion 0.048 U ug/L 6/15/2009 12:20 Field Blank Pesticides Ethoprop 0.097 U ug/L 6/15/2009 12:20 Field Blank Pesticides Delta-BHC 0.0097 U ug/L 6/15/2009 12:20 Field Blank Pesticides Alpha-BHC 0.0097 U ug/L 6/15/2009 12:20 Field Blank Pesticides Endosulfan II 0.019 U ug/L 6/15/2009 12:20 Field Blank Pesticides Endosulfan Sulfate 0.019 U ug/L 6/15/2009 12:20 Field Blank Pesticides Endrin 0.048 U ug/L 
28 

Appendix E. (continued)

Date Sampled Field ID Analysis Group Components Result Remarks Units 

6/15/2009 12:20 Field Blank Pesticides Endrin Aldehyde 0.019 U ug/L 6/15/2009 12:20 Field Blank Pesticides Atrazine 0.048 U ug/L 6/15/2009 12:20 Field Blank Pesticides Metalaxyl 0.24 U ug/L 6/15/2009 12:20 Field Blank Pesticides Metolachlor 0.48 UJ ug/L 6/15/2009 12:20 Field Blank Pesticides Heptachlor Epoxide 0.019 U ug/L 6/15/2009 12:20 Field Blank Pesticides Alachlor 0.58 UJ ug/L 6/15/2009 12:20 Field Blank Pesticides Ametryn 0.048 UJ ug/L 6/15/2009 12:20 Field Blank Pesticides Bromacil 0.19 U ug/L 6/15/2009 12:20 Field Blank Pesticides Norflurazon 0.15 U ug/L 6/15/2009 12:20 Field Blank Pesticides Parathion Methyl 0.097 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants 2,4-Dichlorophenol 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants 4-Nitrophenol 15 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Isophorone 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Hexachlorobenzene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Bis(2-ethylhexyl)phthalate 15 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants 4-Chloro-3-methylphenol 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants 2,4-Dinitrotoluene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Pentachlorophenol 2.9 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Benzo(a)anthracene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Indeno(1,2,3-cd)pyrene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants 1,2,4-Trichlorobenzene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants 2,4,6-Trichlorophenol 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants 2,4-Dimethylphenol 9.7 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Anthracene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Hexachlorobutadiene 2.9 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Nitrobenzene 1.9 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Pyrene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants 2-Methyl-4,6-dinitrophenol 2.9 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Acenaphthylene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Benzo(k)fluoranthene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Diethyl phthalate 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Hexachloroethane 2.9 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants 2,4-Dinitrophenol 15 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants 2-Chloronaphthalene 0.97 U ug/L 
29 

Appendix E. (continued)

Date Sampled Field ID Analysis Group Components Result Remarks Units 

6/15/2009 12:20 Field Blank Priority Organic Pollutants 3,3'-Dichlorobenzidine 39 UJ ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Dibenzo(a,h)anthracene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Hexachlorocyclopentadiene 2.9 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants N-Nitrosodiphenylamine/ Diphenylamine 2.9 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Phenanthrene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants 2-Nitrophenol 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants 4-Chlorophenyl phenyl ether 1.9 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Benzo(b)fluoranthene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Benzo(g,h,i)perylene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Bis(2-chloroethyl)ether 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Chrysene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Fluoranthene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants N-Nitrosodi-n-propylamine 1.9 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants 2,6-Dinitrotoluene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants 4-Bromophenyl phenyl ether 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Bis(2-chloroethoxy)methane 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Butyl benzyl phthalate 4.8 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Di-n-butyl phthalate 4.8 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants N-Nitrosodimethylamine 1.9 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Phenol 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants 2-Chlorophenol 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Acenaphthene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Benzidine 97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Benzo(a)pyrene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Bis(2-chloroisopropyl)ether 2.9 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Di-n-octyl phthalate 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Dimethyl phthalate 9.7 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Fluorene 0.97 U ug/L 6/15/2009 12:20 Field Blank Priority Organic Pollutants Naphthalene 0.97 U ug/L 
30 

Appendix F. Facility Summary, Effluent Limits, and Permit Violations 

Recorded on Discharge Monitoring Report 
31 

Appendix F. (continued) 
32 

Appendix F. (continued) 
33 

Appendix F. (continued) 
34 

Appendix F. (continued) 
35 

Appendix F. (continued) 
36 

Appendix G. EPA End Sheet 

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 0 8 1 7 11 12 0 9 0 6 1 5 18 B 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 0 8 1 7 11 12 0 9 0 6 1 5 18 X 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 0 8 1 7 11 12 0 9 0 6 1 5 18 S 19 S 20 2

66

Biological Analyses of Tampa Electric Company Big Bend Power 

Station effluent sampled on June 15, 2009. NPDES #FL0000817.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

The Bioassay of Tampa Electric Company Big Bend Power Station 

effluent sampled on June 15, 2009. NPDES #FL0000817.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

The Priority Pollutants Analysis of Tampa Electric Company Big 

Bend Power Station effluent sampled on June 15, 2009. NPDES 

#FL0000817.