# Effluent Quality Assessment 

# Tampa Electric Company Big Bend Power Station

Hillsborough County

NPDES #FL0000817

Sampled June 15, 2009

September 2009

Biology Section
Bureau of Laboratories
Division of Environmental Assessment and Restoration

Quality Manual No. 870346G
NELAC Certification No. E31780

## 1 CONTENTS

Introduction

Methods

Results and Discussion

Literature Cited

Tables Table 1 Measured algal growth potential (AGP)
Table 2 Effluent limits, Class III Marine Criteria and chemical data

Appendices Appendix A Chain of Custody Appendix B Bioassay Bench Sheets Appendix C SRT Bench Sheet s and Statistical Analyses Appendix D Organism Receipt Appendix E Chemical Analyses of Effluent Appendix F Facility Summary, Effl uent Limits, and Permit Violations Recorded on Discharge Monitoring Report Appendix G EPA End Sheet

## 2 Tampa Electric Company Big Bend Power Stat ion, 13031 Wyandotte Road, Apollo Beach, Hillsborough County, Florida, 33572. NPDES #F L0000817. Effluent grab samples for bioassay and chemical analyses for this facility were collected on June 15, 2009.



## Introduction Tampa Electric Company Big Bend Power Station is an electric power generating facility which
consists of four coal-fired steam electric units with a total nameplate rating of 1,823 MegaWatts (MW) and three oil-fired combustion turbines wi th a combined capacity of 175 MW. The facility is currently operating under a Consent Order (OGC File # 98-2888D) concerning depressed dissolved oxygen concentrations in the effluent as compared to DO concentrations in the influent. (See Appendix F for detailed facility information provided by Pete Wenner of FDEP Southwest District.)



## Methods Samples were collected from the EFF-1 NPDES sampling point for Outfall D-001 by Jason Cohen and Peter Wenner (FDEP Southwest District) fo llowing DEP-SOP-001/01 FS 2400 Wastewater Sampling. All DEP SOPs are available on the web at: http://www.floridadep.org/labs/qa/sops.htm

.

See Appendix A for the completed chain of custody form.

The 48-hour acute screening toxicity tests and algal growth potential test were performed following internal DEP SOPs TA07_03, TA07_04, and TA08_01 through TA08_09. All internal
DEP SOPs are available on the web at: http://www.floridadep.org/labs/cgi-bin/sop/biosop.asp

.



## Results and Discussion

Toxicity Test Results Tests were performed June 16 - 18, 2009.

Americamysis bahia 48-hour acute screening bioassay – LC 50 

### > 100%, 0%* mortality in 100% sample at 48 hours

Menidia beryllina 48-hour acute screening bioassay – LC 50 

### > 100%, 10%* mortality in 100% sample at 48 hours

The bioassay samples were not acutely toxic to the test organisms.

*NOTE: In a 48-hour acute screening test of 100% effluent, mortality of <20% provides
confidence that the effluent meets the acute to xicity criterion of Florida’s Surface Water Quality Standards (Rule 62-302.500 (1) (a) 4, F. A.C.). Mortality between 20-50% indicates low to moderate levels of toxicity, and furt her action may be required. Mortality of >50%
indicates that the effluent fails to meet the mi nimum requirement to discharge to waters of the state (Rule 62-4.244(3) (a), F.A.C.).

See Appendix B for bioassay bench sheets. See Appendix C for results of the last 20 standard reference toxicant (SRT) tests and the bench sheets and statistical analyses of the SRT tests that correspond to these facility te sts. See Appendix D for test organism receipts.

Algal Growth Potential The effluent Algal Growth Potential (AGP) was 3.0 U (U - Material was analyzed for but not detected; the value reported is the minimum det ection limit) mg dry wt/L of the saltwater

## 3 species Dunaliella tertiolecta. Raschke and Schultz found that AGP values above 10.0 mg dry weight/L represent a “problem” threshold for marine receiving waters, implying nutrient enrichment (personal communication, Marshall Fa ircloth, et. al. FDEP, with Ron Raschke USEPA Region 4 1987). The ratio of total nitr ogen to total phosphorus suggests that the effluent is nitrogen limited (Maloney et al. 1978).

See Table 1 for AGP results.

Chemistry Results Total residual chlorine and total ammonia were not detected in the bioassay sample in the laboratory. The total ammonia concentration in the composite sample collected and preserved for chemical analysis was 0.054 mg N/L. Based on the pH, salinity, and temperature of the effluent as collected, the calculated unioni zed ammonia concentration was < 0.02 mg/L.

Total alpha particles and Combined Radium 226 + 228 were detected in the effluent at levels that comply with Class III marine water quality criteria (62-302.530, F.A.C.). Arsenic, chromium, iron, and nickel were detected between the l aboratory method detection limits (MDL) and practical quantitation limits (PQL). Ortho- phosphate was detected in the field blank.

See Table 2 for results of analytes detected in the effluent, and corresponding limits. See Appendix E for a complete list of chemical analyses performed.



## Conclusion The effluent sample collected from the EFF-1 for this facility on June 15, 2009, was not acutely toxic to either test species during the 48-hour ac ute screening bioassays. The effluent AGP result was less than the "problem” threshold for marine receiving waters. Effluent water quality samples collected on June 15, 2009, did not exceed any appl icable water quality criteria or violate any permit conditions.



## Literature Cited Maloney, T. E., W. E. Miller, and D. T. Specht. 1974. The Marine Algal Assay Procedure: Bottle Test. National Environmental Research Center Office of Research and Development. U. S. EPA, Corvallis, Oregon. 43 p.

## 4 Inor g anic Total

Location N:P ratio N:P ratio

D-001 3.0 U 0.4 4.7 Table 1. Measured algal gr owth potential (AGP; mg dry weight/L) of the saltwater species Dunaliella tertiolecta and ratios of nitrogen to phosphorus for samples collected from TECO Big Bend Power Station on June 15,

2009.

AGP (measured)

U - Material analyzed for but not detected; value reported is

the method detection limit Tampa Electric Company Big Bend Power Station

NPDES # FL0000817

## 5 Tampa Electric Company Big Bend Power

Station NPDES#

FL0000817 Class III

Criteria Effluent

Limits

Aluminum ≤ 1,500 - 180 U Antimony ≤ 4,300 - 1 U

Arsenic ≤ 50 - 1.8 I Beryllium ≤ 0.13 a - 0.075 U
Cadmium ≤ 8.8 - 0.12 U Calcium (mg/L) - - 363
Chromium-III - - 2.8 I Copper ≤ 3.7 - 2 U

Iron ≤ 300 - 170 I

Lead ≤ 8.5 - 0.8 U Magnesium (mg/L) - - 1110

Nickel ≤ 8.3 - 1 I
Selenium ≤ 71 - 0.8 U
Silver ≤ 2.3 - 0.1 U
Zinc ≤ 86 - 15 U

Ortho-phosphate - - 0.13 F Total Phosphorus - - 0.18

Total Ammonia - - 0.054
Un-ionized Ammonia - - 0.01 c
Nitrate and Nitrite - - 0.004 U Total Kjeldahl Nitrogen - - 0.84 Organic Nitrogen - - 0.786 c Total Nitrogen - - 0.842 c

Dissolved Oxygen (mg/L) ≥ 4.0 ≥ 4.0 i 4.3 pH (SU) 6.5 - 8.5 6.5 - 8.5 7.9 Conductivity (μmhos/cm) - - 47,000 Temperature (°C) - 42.78 i 38.5 Salinity - - 30.4 £ Sample Depth (m) - - 0.2 Total Residual Chlorine (mg/L) ≤ 0.01 - ≤ 0.03 £ Chlorophyll a (μg/L) - Corrected -- 3 Phaeophytin (μg/L) - - 1.3 Total Suspended Solids (mg/L) - - 7 I CBOD, 5 day (mg/L), N - inhibited - - 1.4 I Oils and Greases (mg/L) ≤ 5.0 - 1.4 U Alpha, Total (pCi/L) ≤ 15 - 2.1 Alpha-Counting Error (pCi/L) - - 1.5 Radium 226 (pCi/L) - - 1 Radium 226-Counting Error (pCi/L) - - 0.2 Radium 228 (pCi/L) - - 0.9 U Radium 228-Counting Error (pCi/L) - - 0.6 Radium 226 + 228 (pCi/L) ≤ 5 - 1c Flow (MGD) - Report 1121.01 a Hardness (mg CaCO 3 ) - - 5477.39 c

Value exceeds the Class III Water Quality Criteria or permit limits a - Annual average
c - Value is calculated

i - Instantateous Maximum F - Analyte was detected in both sample and field blank I - The reported value is between the laboratory method detection limit and the laboratory practical quantitation limit U - Material analyzed for but not detected; value reported is the method detection limit
£ - Measured in Central labs and entered into Tox sheets Table 2. Effluent limits, Class III Criteria for predominantly marine waters and chemical data for samples collected from the TECO Big Bend Power Station on June 15, 2009.

Nutrients (mg/L)

General Physical and Chemical Parameters Effluent

Metals (μg/L unless otherwise noted)

## 6 Appendix A. Chain of Custody Form

## 7 Appendix B. Bench Sheets

## 8 Appendix B. (continued)

## 9 Appendix B. (continued)

## 10 Appendix B. (continued)

## 11 Appendix B. (continued)

## 12 Appendix B. (continued)

## 13 Appendix C. SRT Bench Sheet s and Statistical Analysis
Florida Department of Environmental Protection
Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - 48-hour Acute Toxicity Americamysis bahia (mysid shrimp) - Cadmium Chloride (CdCl 2 

### )

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit
2/5/2008 * 23.52 50.68 13.02 24.64 76.72
02/12/08 36.63 49.45 13.14 23.17 75.74
03/11/08 38.21 47.42 11.38 24.66 70.19
04/01/08 53.27 46.90 10.82 25.26 68.53
05/06/08 35.65 45.82 10.81 24.19 67.45
06/03/08 58.28 46.36 11.17 24.03 68.69
07/08/08 47.67 46.00 10.99 24.02 67.98
08/05/08 44.68 46.32 10.84 24.63 68.01
09/03/08 45.94 45.82 10.60 24.62 67.02
10/07/08 41.43 44.39 8.96 26.47 62.30
11/04/08 46.75 44.58 8.97 26.64 62.51
11/12/09 44.92 44.87 8.87 27.13 62.61
12/02/08 40.59 44.42 8.84 26.73 62.11
01/06/09 37.22 43.80 8.89 26.01 61.59
02/03/09 39.02 43.57 8.96 25.66 61.49
03/03/09 30.58 42.81 9.40 24.02 61.61
03/31/09 38.24 42.65 9.45 23.76 61.55
04/08/09 31.38 42.44 9.66 23.12 61.76
05/05/09 33.86 41.62 9.66 22.31 60.93
06/09/09 41.42 40.46 8.00 24.47 56.45

Lower Confidence Limit = mean - 2 * Std Dev CV = 19.76 Upper Confidence Limit = mean + 2 * Std Dev EPA reference 75th quartile CV = 26%



### Standard Reference Toxicant Data Americamysis bahia 48-hour LC 50



### 20 30 40 50 60 70 802/5/2008 *02/12/0803/11/0804/01/0805/06/0806/03/0807/08/0808/05/0809/03/0810/07/0811/04/0811/12/0912/02/0801/06/0902/03/0903/03/0903/31/0904/08/0905/05/0906/09/09



### Test Dateμg/L CdCl2

LC50 Mean LC50 Lower Control Limit Upper Control Limit

## 14 Appendix C. (continued) Florida Department of Environmental Protection
Bureau of Laboratories

Standard Reference Toxicant ( SRT ) Test Data - Acute Toxicit y M

en idi a b ery lli

na (I n l an d s il vers id e ) - C opper Chl or id e (C u Cl 2 

## )

Date LC50 Mean LC50 Std Dev Lower
Control Limit Upper
Control Limit

01/08/08 329.03 285.29 71.1 143.0 427.5
02/05/08 357.47 294.07 68.5 157.1 431.0
03/04/08 295.52 295.31 68.1 159.3 431.9
04/01/08 379.50 304.05 67.5 169.0 439.1
05/06/08 272.50 304.73 67.1 170.5 438.9
06/03/08 373.47 312.52 65.5 181.5 443.5
07/08/08 275.20 316.13 61.0 194.2 438.1
08/12/08 221.17 320.17 50.5 219.2 421.1
09/03/08 167.46 313.48 60.9 191.7 435.3
09/09/08 240.07 311.58 62.6 186.3 436.9 10/07/08 325.55 312.05 62.7 186.6 437.5 11/04/08 249.81 307.63 63.9 179.9 435.4 12/02/08 316.79 310.59 62.8 185.0 436.2 01/06/09 257.04 311.08 62.3 186.5 435.7 02/03/09 245.51 305.47 62.9 179.6 431.4 03/03/09 433.98 309.64 68.6 172.5 446.8 03/31/09 228.94 304.17 70.5 163.1 445.2 05/05/09 310.12 302.74 70.1 162.6 442.9 06/02/09 471.25 305.32 75.4 154.6 456.0 06/09/09 370.27 306.03 75.9 154.2 457.9

Means are of the last 20 SRT tests. EPA reference 75th quartile CV = 21% CV = 24.81



## Standard Reference Toxicant Data

Menidia beryllina 48 hour LC50

0 50 100 150 200 250 300 350 400 450 50001/08/0802/05/0803/04/0804/01/0805/06/0806/03/0807/08/0808/12/0809/03/0809/09/0810/07/0811/04/0812/02/0801/06/0902/03/0903/03/0903/31/0905/05/0906/02/0906/09/09



## Test Dateμg/L CuCl2



### Lower Control Limit LC50 Mean LC50 Upper Control Limit

## 15 Appendix C. (continued)

## 16 Appendix C. (continued)

## 17 Appendix C. (continued)

## 18 Appendix C. (continued)

## 19 Appendix C. (continued)



## A mericam y sis bahia Acute Standard Reference Toxicant Test

DATE: 6-9-09 DURATION: 48 hr TOXICANT: CdCl2
SPECIES: Americamysis bahia

RAW DATA: Concentration Number Mortalities (ug/L) Exposed
0.00 20 0
20.00 20 0
30.00 20 2
45.00 20 14
67.00 20 18
100.00 20 20

SPEARMAN-KARBER TRIM: .00%

SPEARMAN-KARBER ESTIMATES: LC50: 41.42
95% LO WER CONFIDENCE: 37.02
95% UPPE R CONFIDENCE: 46.35

M enidia ber y llina Acute Standard Reference Toxicant Test

DATE: 6-9-09 DURATION: 48 hr TOXICANT: CuCl2
SPECIES: Menidia beryllina

RAW DATA: Concentration Number Mortalities (ug/l) Exposed
0.00 20 0
100.00 20 0
200.00 20 0
300.00 20 5
400.00 20 8
500.00 20 20

SPEARMAN-KARBER TRIM: .00%

SPEARMAN-KARBER ESTIMATES: LC50: 370.27
95% LOWE R CONFIDENCE: 339.29
95% UPPER CONFIDENCE: 404.08

## 20 Appendix D. Test Organism Receipts

## 21 Appendix D. (continued)22Appendix E. Chemical Analyses performed on the effluent from Tampa Electric Company BigBend Power StationOutfall D-001, sampled on June 15, 2009.Date Sampled Field ID Analysis Group Components Result Remarks Units MDL PQL6/15/2009 10:30 37693 AGP/LN Algal Growth Potential 3 U mg DryWt/L 3 36/15/2009 10:30 37693 Chlorophyll/Grain Size/BOD Chlorophyll-a, Corrected 3 ug/L 0.55 1.76/15/2009 10:30 37693 Chlorophyll/Grain Size/BOD Biochemical Oxygen Demand-5 Day,N-Inhib 1.4 I mg/L 0.2 26/15/2009 10:30 37693 Chlorophyll/Grain Size/BOD Phaeophytin-a 1.3 ug/L 0.4 1.26/15/2009 10:30 37693 Toxicology Bioassay-Acute-Screen-SW-Fish, LC50 100 L LC506/15/2009 10:30 37693 Toxicology Bioassay-Acute-Screen-SW-Mysid, LC50 100 L LC506/15/2009 10:30 37693 Metals Arsenic 1.8 I ug/L 1 46/15/2009 10:30 37693 Metals Zinc 15 U ug/L 15 606/15/2009 10:30 37693 Metals Beryllium 0.075 U ug/L 0.075 0.36/15/2009 10:30 37693 Metals Antimony 1 U ug/L 1 46/15/2009 10:30 37693 Metals Aluminum 180 U ug/L 180 7206/15/2009 10:30 37693 Metals Nickel 1 I ug/L 1 46/15/2009 10:30 37693 Metals Chromium 2.8 I ug/L 1.2 4.86/15/2009 10:30 37693 Metals Calcium 363 mg/L 0.22 0.96/15/2009 10:30 37693 Metals Silver 0.1 U ug/L 0.1 0.46/15/2009 10:30 37693 Metals Selenium 0.8 U ug/L 0.8 3.26/15/2009 10:30 37693 Metals Magnesium 1110 mg/L 2 86/15/2009 10:30 37693 Metals Iron 170 I ug/L 90 3606/15/2009 10:30 37693 Metals Copper 2 U ug/L 2 26/15/2009 10:30 37693 Metals Cadmium 0.12 U ug/L 0.12 0.486/15/2009 10:30 37693 Metals Lead 0.8 U ug/L 0.8 3.26/15/2009 10:30 37693 Nutrients Ammonia-N 0.054 mg N/L 0.01 0.026/15/2009 10:30 37693 Nutrients Total-P 0.18 mg P/L 0.008 0.026/15/2009 10:30 37693 Nutrients NO2NO3-N 0.004 U mg N/L 0.004 0.016/15/2009 10:30 37693 Nutrients TSS 7 I mg/L 4 166/15/2009 10:30 37693 Nutrients Kjeldahl Nitrogen 0.84 mg N/L 0.16 0.46/15/2009 10:30 37693 Nutrients O-Phosphate-P 0.13 mg P/L 0.008 0.026/15/2009 10:30 37693 Overflow Radium 228-Counting Error 0.6 pCi/L6/15/2009 10:30 37693 Overflow Oil and Grease 1.4 U mg/L 1.4 56/15/2009 10:30 37693 Overflow Radium 228 0.9 U pCi/L6/15/2009 10:30 37693 Overflow Alpha, Total 2.1 pCi/L6/15/2009 10:30 37693 Overflow Radium 226 1 pCi/L23Appendix E. (continued)Date Sampled Field ID Analysis Group Components Result Remarks Units MDL PQL6/15/2009 10:30 37693 Overflow Alpha-Counting Error 1.5 pCi/L6/15/2009 10:30 37693 Overflow Radium 226-Counting Error 0.2 pCi/L6/15/2009 10:30 37693 Pesticides Endosulfan Sulfate 0.02 U ug/L 0.02 0.086/15/2009 10:30 37693 Pesticides Norflurazon 0.15 U ug/L 0.15 0.66/15/2009 10:30 37693 Pesticides Aldrin 0.01 U ug/L 0.01 0.046/15/2009 10:30 37693 Pesticides Delta-BHC 0.01 U ug/L 0.01 0.046/15/2009 10:30 37693 Pesticides Metalaxyl 0.26 U ug/L 0.26 16/15/2009 10:30 37693 Pesticides Mevinphos 0.2 U ug/L 0.2 0.86/15/2009 10:30 37693 Pesticides Parathion Ethyl 0.15 U ug/L 0.15 0.66/15/2009 10:30 37693 Pesticides Simazine 0.051 U ug/L 0.051 0.26/15/2009 10:30 37693 Pesticides Endosulfan I 0.02 U ug/L 0.02 0.086/15/2009 10:30 37693 Pesticides Toxaphene 0.77 U ug/L 0.77 3.16/15/2009 10:30 37693 Pesticides Bromacil 0.2 U ug/L 0.2 0.86/15/2009 10:30 37693 Pesticides Ethoprop 0.1 U ug/L 0.1 0.46/15/2009 10:30 37693 Pesticides Metolachlor 0.51 UJ ug/L 0.51 26/15/2009 10:30 37693 Pesticides Naled 0.82 U ug/L 0.82 3.36/15/2009 10:30 37693 Pesticides DDD-p,p' 0.02 U ug/L 0.02 0.086/15/2009 10:30 37693 Pesticides DDE-p,p' 0.02 U ug/L 0.02 0.086/15/2009 10:30 37693 Pesticides Dieldrin 0.02 U ug/L 0.02 0.086/15/2009 10:30 37693 Pesticides Endrin 0.051 U ug/L 0.051 0.26/15/2009 10:30 37693 Pesticides Atrazine 0.051 U ug/L 0.051 0.26/15/2009 10:30 37693 Pesticides Azinphos Methyl 0.2 UJ ug/L 0.2 0.86/15/2009 10:30 37693 Pesticides Chlorpyrifos Ethyl 0.051 U ug/L 0.051 0.26/15/2009 10:30 37693 Pesticides Ethion 0.051 U ug/L 0.051 0.26/15/2009 10:30 37693 Pesticides Alpha-BHC 0.01 U ug/L 0.01 0.046/15/2009 10:30 37693 Pesticides DDT-p,p' 0.031 U ug/L 0.031 0.126/15/2009 10:30 37693 Pesticides Endosulfan II 0.02 U ug/L 0.02 0.086/15/2009 10:30 37693 Pesticides Diazinon 0.051 U ug/L 0.051 0.26/15/2009 10:30 37693 Pesticides Fenamiphos 0.2 U ug/L 0.2 0.86/15/2009 10:30 37693 Pesticides Malathion 0.15 U ug/L 0.15 0.66/15/2009 10:30 37693 Pesticides Phorate 0.051 U ug/L 0.051 0.26/15/2009 10:30 37693 Pesticides Endrin Aldehyde 0.02 U ug/L 0.02 0.086/15/2009 10:30 37693 Pesticides Gamma-BHC 0.01 U ug/L 0.01 0.046/15/2009 10:30 37693 Pesticides Alachlor 0.61 UJ ug/L 0.61 2.424Appendix E. (continued)Date Sampled Field ID Analysis Group Components Result Remarks Units MDL PQL6/15/2009 10:30 37693 Pesticides Hexazinone 0.1 U ug/L 0.1 0.46/15/2009 10:30 37693 Pesticides Beta-BHC 0.01 U ug/L 0.01 0.046/15/2009 10:30 37693 Pesticides Heptachlor 0.01 U ug/L 0.01 0.046/15/2009 10:30 37693 Pesticides Heptachlor Epoxide 0.02 U ug/L 0.02 0.086/15/2009 10:30 37693 Pesticides Methoxychlor 0.051 U ug/L 0.051 0.26/15/2009 10:30 37693 Pesticides Ametryn 0.051 UJ ug/L 0.051 0.26/15/2009 10:30 37693 Pesticides Butylate 0.2 UJ ug/L 0.2 0.86/15/2009 10:30 37693 Pesticides Chlorpyrifos Methyl 0.1 U ug/L 0.1 0.46/15/2009 10:30 37693 Pesticides Fonofos 0.1 UJ ug/L 0.1 0.46/15/2009 10:30 37693 Pesticides Metribuzin 0.1 U ug/L 0.1 0.46/15/2009 10:30 37693 Pesticides Parathion Methyl 0.1 U ug/L 0.1 0.46/15/2009 10:30 37693 Pesticides Prometryn 0.15 UJ ug/L 0.15 0.66/15/2009 10:30 37693 Pesticides Chlordane 0.2 U ug/L 0.2 0.86/15/2009 10:30 37693 Priority Organic Pollutants Benzidine 94 U ug/L 94 3806/15/2009 10:30 37693 Priority Organic Pollutants Dibenzo(a,h)anthracene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Diethyl phthalate 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Hexachlorobutadiene 2.8 U ug/L 2.8 116/15/2009 10:30 37693 Priority Organic Pollutants Hexachloroethane 2.8 U ug/L 2.8 116/15/2009 10:30 37693 Priority Organic Pollutants N-Nitrosodi-n-propylamine 1.9 U ug/L 1.9 7.66/15/2009 10:30 37693 Priority Organic Pollutants N-Nitrosodiphenylamine/ Diphenylamine 2.8 U ug/L 2.8 116/15/2009 10:30 37693 Priority Organic Pollutants Pyrene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Hexachlorobenzene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic PollutantsIndeno(1,2,3-cd)pyrene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants N-Nitrosodimethylamine 1.9 U ug/L 1.9 7.66/15/2009 10:30 37693 Priority Organic Pollutants Nitrobenzene 1.9 U ug/L 1.9 7.66/15/2009 10:30 37693 Priority Organic Pollutants 2-Methyl-4,6-dinitrophenol 2.8 U ug/L 2.8 116/15/2009 10:30 37693 Priority Organic Pollutants 4-Chlorophenyl phenyl ether 1.9 U ug/L 1.9 7.66/15/2009 10:30 37693 Priority Organic Pollutants Di-n-octyl phthalate 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Phenanthrene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants 2-Chloronaphthalene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants 2-Chlorophenol 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants 2-Nitrophenol 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants 3,3'-Dichlorobenzidine 38 UJ ug/L 38 15025Appendix E. (continued)Date Sampled Field ID Analysis Group Components Result Remarks Units MDL PQL6/15/2009 10:30 37693 Priority Organic Pollutants 4-Bromophenyl phenyl ether 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants 4-Nitrophenol 14 U ug/L 14 576/15/2009 10:30 37693 Priority Organic Pollutants Bis(2-ethylhexyl)phthalate 14 U ug/L 14 576/15/2009 10:30 37693 Priority Organic Pollutants Chrysene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Di-n-butyl phthalate 4.7 U ug/L 4.7 196/15/2009 10:30 37693 Priority Organic Pollutants Dimethyl phthalate 9.4 U ug/L 9.4 386/15/2009 10:30 37693 Priority Organic Pollutants Fluoranthene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Fluorene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Isophorone 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Naphthalene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants 2,4-Dichlorophenol 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants 4-Chloro-3-methylphenol 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Benzo(g,h,i)perylene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Anthracene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Benzo(k)fluoranthene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Butyl benzyl phthalate 4.7 U ug/L 4.7 196/15/2009 10:30 37693 Priority Organic Pollutants Pentachlorophenol 2.8 U ug/L 2.8 116/15/2009 10:30 37693 Priority Organic Pollutants 2,4-Dinitrophenol 14 U ug/L 14 576/15/2009 10:30 37693 Priority Organic Pollutants Acenaphthene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Acenaphthylene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Benzo(a)anthracene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Benzo(b)fluoranthene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Bis(2-chloroethoxy)methane 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Phenol 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants 1,2,4-Trichlorobenzene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants 2,4,6-Trichlorophenol 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants 2,4-Dimethylphenol 9.4 U ug/L 9.4 386/15/2009 10:30 37693 Priority Organic Pollutants 2,4-Dinitrotoluene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants 2,6-Dinitrotoluene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Benzo(a)pyrene 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Bis(2-chloroethyl)ether 0.94 U ug/L 0.94 3.86/15/2009 10:30 37693 Priority Organic Pollutants Bis(2-chloroisopropyl)ether 2.8 U ug/L 2.8 116/15/2009 10:30 37693 Priority Organic Pollutants Hexachlorocyclopentadiene 2.8 UJ ug/L 2.8 1126Appendix E. (continued)Date Sampled Field ID Analysis Group Components Result Remarks Units MDL PQL6/15/2009 10:30 37693 Field Parameter Sample Depth 0.2 m6/15/2009 10:30 37693 Field Parameter Temperature 38.5 C6/15/2009 10:30 37693 Field Parameter Specific Conductance 47000 umhos/cm6/15/2009 10:30 37693 Field Parameter Dissolved Oxygen 4.32 mg/L6/15/2009 10:30 37693 Field Parameter pH 7.946/15/2009 12:20 Field Blank Chlorophyll/Grain Size/BOD Biochemical Oxygen Demand-5 Day,N-Inhib 0.2 U mg/L 0.2 26/15/2009 12:20 Field Blank Metals Aluminum 60 U ug/L 60 2406/15/2009 12:20 Field Blank Metals Cadmium 0.03 U ug/L 0.03 0.126/15/2009 12:20 Field Blank Metals Chromium 0.3 U ug/L 0.3 1.26/15/2009 12:20 Field Blank Metals Silver 0.025 U ug/L 0.025 0.16/15/2009 12:20 Field Blank Metals Nickel 0.25 U ug/L 0.25 16/15/2009 12:20 Field Blank Metals Antimony 0.25 U ug/L 0.25 16/15/2009 12:20 Field Blank Metals Selenium 0.2 U ug/L 0.2 0.86/15/2009 12:20 Field Blank Metals Calcium 0.075 U mg/L 0.075 0.36/15/2009 12:20 Field Blank Metals Magnesium 0.04 U mg/L 0.04 0.166/15/2009 12:20 Field Blank Metals Zinc 5 U ug/L 5 206/15/2009 12:20 Field Blank Metals Arsenic 0.25 U ug/L 0.25 16/15/2009 12:20 Field Blank Metals Iron 30 U ug/L 30 1206/15/2009 12:20 Field Blank Metals Lead 0.2 U ug/L 0.2 0.86/15/2009 12:20 Field Blank Metals Copper 0.5 U ug/L 0.5 0.56/15/2009 12:20 Field Blank Metals Beryllium 0.025 U ug/L 0.025 0.16/15/2009 12:20 Field Blank Nutrients O-Phosphate-P 0.006 I mg P/L 0.004 0.016/15/2009 12:20 Field Blank Nutrients Total-P 0.004 U mg P/L 0.004 0.016/15/2009 12:20 Field Blank Nutrients NO2NO3-N 0.004 U mg N/L 0.004 0.016/15/2009 12:20 Field Blank Nutrients Ammonia-N 0.01 U mg N/L 0.01 0.026/15/2009 12:20 Field Blank Nutrients Kjeldahl Nitrogen 0.08 U mg N/L 0.08 0.26/15/2009 12:20 Field Blank Overflow Radium 228 0.9 U pCi/L6/15/2009 12:20 Field Blank Overflow Radium 226 0.2 U pCi/L6/15/2009 12:20 Field Blank Overflow Radium 226-Counting Error 0.1 pCi/L6/15/2009 12:20 Field Blank Overflow Oil and Grease 1.4 U mg/L 1.4 56/15/2009 12:20 Field Blank Overflow Radium 228-Counting Error 0.5 pCi/L6/15/2009 12:20 Field Blank Overflow Alpha-Counting Error 0.5 pCi/L6/15/2009 12:20 Field Blank Overflow Alpha, Total 0.9 U pCi/L6/15/2009 12:20 Field Blank Pesticides Azinphos Methyl 0.19 UJ ug/L 0.19 0.7627Appendix E. (continued)Date Sampled Field ID Analysis Group Components Result Remarks Units MDL PQL6/15/2009 12:20 Field Blank Pesticides Hexazinone 0.097 U ug/L 0.097 0.396/15/2009 12:20 Field Blank Pesticides Naled 0.78 U ug/L 0.78 3.16/15/2009 12:20 Field Blank Pesticides Prometryn 0.15 UJ ug/L 0.15 0.66/15/2009 12:20 Field Blank Pesticides Beta-BHC 0.0097 U ug/L 0.0097 0.0396/15/2009 12:20 Field Blank Pesticides Heptachlor 0.0097 U ug/L 0.0097 0.0396/15/2009 12:20 Field Blank Pesticides Butylate 0.19 UJ ug/L 0.19 0.766/15/2009 12:20 Field Blank Pesticides Chlorpyrifos Ethyl 0.048 U ug/L 0.048 0.196/15/2009 12:20 Field Blank Pesticides Mevinphos 0.19 U ug/L 0.19 0.766/15/2009 12:20 Field Blank Pesticides Parathion Ethyl 0.15 U ug/L 0.15 0.66/15/2009 12:20 Field Blank Pesticides Simazine 0.048 U ug/L 0.048 0.196/15/2009 12:20 Field Blank Pesticides Endosulfan I 0.019 U ug/L 0.019 0.0766/15/2009 12:20 Field Blank Pesticides Diazinon 0.048 U ug/L 0.048 0.196/15/2009 12:20 Field Blank Pesticides Chlordane 0.19 U ug/L 0.19 0.766/15/2009 12:20 Field Blank Pesticides DDD-p,p' 0.019 U ug/L 0.019 0.0766/15/2009 12:20 Field Blank Pesticides DDT-p,p' 0.029 U ug/L 0.029 0.126/15/2009 12:20 Field Blank Pesticides Toxaphene 0.73 U ug/L 0.73 2.96/15/2009 12:20 Field Blank Pesticides Chlorpyrifos Methyl 0.097 U ug/L 0.097 0.396/15/2009 12:20 Field Blank Pesticides Fenamiphos 0.19 U ug/L 0.19 0.766/15/2009 12:20 Field Blank Pesticides Fonofos 0.097 UJ ug/L 0.097 0.396/15/2009 12:20 Field Blank Pesticides Malathion 0.15 U ug/L 0.15 0.66/15/2009 12:20 Field Blank Pesticides Metribuzin 0.097 U ug/L 0.097 0.396/15/2009 12:20 Field Blank Pesticides Phorate 0.048 U ug/L 0.048 0.196/15/2009 12:20 Field Blank Pesticides Aldrin 0.0097 U ug/L 0.0097 0.0396/15/2009 12:20 Field Blank Pesticides DDE-p,p' 0.019 U ug/L 0.019 0.0766/15/2009 12:20 Field Blank Pesticides Dieldrin 0.019 U ug/L 0.019 0.0766/15/2009 12:20 Field Blank Pesticides Gamma-BHC 0.0097 U ug/L 0.0097 0.0396/15/2009 12:20 Field Blank Pesticides Methoxychlor 0.048 U ug/L 0.048 0.196/15/2009 12:20 Field Blank Pesticides Ethion 0.048 U ug/L 0.048 0.196/15/2009 12:20 Field Blank Pesticides Ethoprop 0.097 U ug/L 0.097 0.396/15/2009 12:20 Field Blank PesticidesDelta-BHC 0.0097 U ug/L 0.0097 0.0396/15/2009 12:20 Field Blank Pesticides Alpha-BHC 0.0097 U ug/L 0.0097 0.0396/15/2009 12:20 Field Blank Pesticides Endosulfan II 0.019 U ug/L 0.019 0.0766/15/2009 12:20 Field Blank Pesticides Endosulfan Sulfate 0.019 U ug/L 0.019 0.0766/15/2009 12:20 Field Blank Pesticides Endrin 0.048 U ug/L 0.048 0.1928Appendix E. (continued)Date Sampled Field ID Analysis Group Components Result Remarks Units MDL PQL6/15/2009 12:20 Field Blank Pesticides Endrin Aldehyde 0.019 U ug/L 0.019 0.0766/15/2009 12:20 Field Blank Pesticides Atrazine 0.048 U ug/L 0.048 0.196/15/2009 12:20 Field Blank Pesticides Metalaxyl 0.24 U ug/L 0.24 0.966/15/2009 12:20 Field Blank Pesticides Metolachlor 0.48 UJ ug/L 0.48 1.96/15/2009 12:20 Field Blank Pesticides Heptachlor Epoxide 0.019 U ug/L 0.019 0.0766/15/2009 12:20 Field Blank Pesticides Alachlor 0.58 UJ ug/L 0.58 2.36/15/2009 12:20 Field Blank Pesticides Ametryn 0.048 UJ ug/L 0.048 0.196/15/2009 12:20 Field Blank PesticidesBromacil 0.19 U ug/L 0.19 0.766/15/2009 12:20 Field Blank Pesticides Norflurazon 0.15 U ug/L 0.15 0.66/15/2009 12:20 Field Blank Pesticides Parathion Methyl 0.097 U ug/L 0.097 0.396/15/2009 12:20 Field Blank Priority Organic Pollutants 2,4-Dichlorophenol 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants 4-Nitrophenol 15 U ug/L 15 586/15/2009 12:20 Field Blank Priority Organic Pollutants Isophorone 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Hexachlorobenzene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Bis(2-ethylhexyl)phthalate 15 U ug/L 15 586/15/2009 12:20 Field Blank Priority Organic Pollutants 4-Chloro-3-methylphenol 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants 2,4-Dinitrotoluene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Pentachlorophenol 2.9 U ug/L 2.9 126/15/2009 12:20 Field Blank Priority Organic Pollutants Benzo(a)anthracene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Indeno(1,2,3-cd)pyrene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants 1,2,4-Trichlorobenzene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants 2,4,6-Trichlorophenol 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants 2,4-Dimethylphenol 9.7 U ug/L 9.7 396/15/2009 12:20 Field Blank Priority Organic Pollutants Anthracene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Hexachlorobutadiene 2.9 U ug/L 2.9 126/15/2009 12:20 Field Blank Priority Organic Pollutants Nitrobenzene 1.9 U ug/L 1.9 7.76/15/2009 12:20 Field Blank Priority Organic Pollutants Pyrene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants 2-Methyl-4,6-dinitrophenol 2.9 U ug/L 2.9 126/15/2009 12:20 Field Blank Priority Organic Pollutants Acenaphthylene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Benzo(k)fluoranthene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Diethyl phthalate 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Hexachloroethane 2.9 U ug/L 2.9 126/15/2009 12:20 Field Blank Priority Organic Pollutants 2,4-Dinitrophenol 15 U ug/L 15 586/15/2009 12:20 Field Blank Priority Organic Pollutants 2-Chloronaphthalene 0.97 U ug/L 0.97 3.929Appendix E. (continued)Date Sampled Field ID Analysis Group Components Result Remarks Units MDL PQL6/15/2009 12:20 Field Blank Priority Organic Pollutants 3,3'-Dichlorobenzidine 39 UJ ug/L 39 1506/15/2009 12:20 Field Blank Priority Organic Pollutants Dibenzo(a,h)anthracene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Hexachlorocyclopentadiene 2.9 U ug/L 2.9 126/15/2009 12:20 Field Blank Priority Organic Pollutants N-Nitrosodiphenylamine/ Diphenylamine 2.9 U ug/L 2.9 126/15/2009 12:20 Field Blank Priority Organic Pollutants Phenanthrene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants 2-Nitrophenol 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants 4-Chlorophenyl phenyl ether 1.9 U ug/L 1.9 7.76/15/2009 12:20 Field Blank Priority Organic Pollutants Benzo(b)fluoranthene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Benzo(g,h,i)perylene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Bis(2-chloroethyl)ether 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Chrysene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Fluoranthene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants N-Nitrosodi-n-propylamine 1.9 U ug/L 1.9 7.76/15/2009 12:20 Field Blank Priority Organic Pollutants 2,6-Dinitrotoluene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants 4-Bromophenyl phenyl ether 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Bis(2-chloroethoxy)methane 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Butyl benzyl phthalate 4.8 U ug/L 4.8 196/15/2009 12:20 Field Blank Priority Organic Pollutants Di-n-butyl phthalate 4.8 U ug/L 4.8 196/15/2009 12:20 Field Blank Priority Organic Pollutants N-Nitrosodimethylamine 1.9 U ug/L 1.9 7.76/15/2009 12:20 Field Blank Priority Organic Pollutants Phenol 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants 2-Chlorophenol 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Acenaphthene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Benzidine 97 U ug/L 97 3906/15/2009 12:20 Field Blank Priority Organic Pollutants Benzo(a)pyrene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Bis(2-chloroisopropyl)ether 2.9 U ug/L 2.9 126/15/2009 12:20 Field Blank Priority Organic Pollutants Di-n-octyl phthalate 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Dimethyl phthalate 9.7 U ug/L 9.7 396/15/2009 12:20 Field Blank Priority Organic Pollutants Fluorene 0.97 U ug/L 0.97 3.96/15/2009 12:20 Field Blank Priority Organic Pollutants Naphthalene 0.97 U ug/L 0.97 3.9

## 30 Appendix F. Facility Summary, Effl uent Limits, and Permit Violations Recorded on Discharge Monitoring Report

## 31 Appendix F. (continued)

## 32 Appendix F. (continued)

## 33 Appendix F. (continued)

## 34 Appendix F. (continued)

## 35 Appendix F. (continued)

## 36 Appendix G. EPA End Sheet

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 0 8 1 7 11 12 0 9 0 6 1 5 18 B 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 0 8 1 7 11 12 0 9 0 6 1 5 18 X 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 0 8 1 7 11 12 0 9 0 6 1 5 18 S 19 S 20 2

66 

# Biological Analyses of Tampa Electric Company Big Bend Power Station effluent sampled on J une 15, 2009. NP DES #FL0000817.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks 

# The Bioassay of Tampa Electric Company Big Bend Power Station effluent sampled on June 15, 2009. NPDE S #FL0000817.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks



# The Priority Pollutants Analysis of Tampa Electric Company Big Bend Power Station effluent sampled on June 15, 2009. NPDES

#FL0000817.