## Effluent Quality Assessment 

# Florida Power and Light Company Fort Myers Plant

Lee County

NPDES #FL0001490 Sampled July 21, 2008

November 2008

Biology Section Bureau of Laboratories Division of Environmental Assessment and Restoration

Quality Manual No. 870346G NELAC Certification No. E31780

### 1 Florida Power and Light Company – Fort Myers Plant, 10650 State Road 80, Fort Myers, Lee County, Florida, 33905. NPDES #FL0001490. Efflu ent samples for this facility were collected on July 21, 2008.



### Introduction Florida Power and Light Company – Fort Myers Pl ant is an electric power generating plant. The plant consists of:

• six natural gas combined-cycle combustion tu rbines with a total generating capacity of 1,550 megawatts (MW);

• associated Heat-Recov er Steam Generators and steam turbines;

• twelve gas turbine peaking units with a combined generating capacity of 600 (MW);

• two peaker combustion turbines with a combined generating capacity of 326 (MW);

• a once-through condenser cooling water system;

• a once-through helper cooling tower;

• a stormwater basin;

• an evaporation/percolation pond;

• ground water monitoring wells; and,

• a reverse osmosis plant.

The facility does not have a design flow. The comb ined discharge (once through cooling water, RO plant discharge, auxiliary equipment cooling water, etc.) from the plant from July 2007 through June 2008 was approximately 670.3 milli on gallons per day (MGD). Surface water discharge occurs at the end of the 762 M (2,500 feet) long on-site discharge canal at Outfall D010. The receiving water is the Class III Marine waters of the Orange River (Note: the Orange River is designated as Class III freshwater in t he facility’s permit; howev er, watershed biologists classify this portion of the river as tidal marine). The point of discharge is located approximately at latitude 26o 41’ 31” N, longitude 81o 46’ 41” W. Wa stewater treatment at the facility consists of routing equipment-area stormwater and washdown water that have the potential to contain oil and grease through an oil/water separator prior to disc harge to the stormwater basin. The contents of the stormwater basin are pumped to the evaporati on/percolation basin. Fuel oil storage runoff is administratively controlled by visual inspection s prior to discharge to the evaporation/percolation basin. Metal cleaning wastewater is disposed of offsite.

The facility’s once-through condenser cooling wate r system uses water from the Caloosahatchee River as intake water. Cooling water is routed th rough the plant condensers and is then discharged to the discharge canal. A once-through cooling tower, located adjacent to the discharge canal, is used to reduce the discharge temperature of the cooling water. Boiler blowdown is captured and reused. Reverse osmosis reject is discharged indirectly to the discharge canal via an internal discharge to the cooling tower basin.

The facility has no permitted toxicity test require ments; however, a 48-hour acute screening test can provide confidence that the effluent meets t he acute toxicity criterion of Florida’s Surface Water Quality Standards (Rule 62-302.500 (1)(a)4, F.A. C.) if the mortality in 100% effluent is less than 20%.

The facility has no Administrative Orders, Consent Orders or mixing zones. A review of Discharge Monitoring Report data showed no permit violations for the past year. (Facility information from NPDES permit #FL0001490 and Alyssa Mork FDEP South District.)



### Methods Samples were collected from the EFF-1 NPDES sa mpling point for Outfall D-010 by Alyssa Mork (FDEP South District) following DEP-SOP-001/01 FS 2400 Wastewater Sampling. All DEP SOPs are available on the web at: http://www.floridadep.org/labs/qa/sops.htm

.

### 2 See Appendix A for the completed chain of custody form.

The 48 - hour acute screening toxicity tests an d algal growth potential test were performed following internal DEP SOPs TA07_01, TA07_02, and TA08_01 through TA08_09. All internal DEP SOPs are available on the web at: http://www.floridadep.org/labs/cgi-bin/sop/biosop.asp

.
An aliquot of the AGP sample was salinity adjust ed to meet the minimum salinity requirements of the marine test species. Both a freshwater AGP analysis and a marine AGP analysis were performed.



### Toxicity Test Results Tests were performed July 22 - 24, 2008.

Ceriodaphnia dubia 48-hour acute screening bioassay – LC 50 > 100%, 0% mortality* in 100% sample at 48 hours

Cyprinella leedsi 48-hour acute screening bioassay – LC 50 > 100%, 5% mortality* in 100% sample at 48 hours

The bioassay samples were not acutely toxic to the test organisms.

See Appendix B for bioassay bench sheets. See Ap pendix C for results of the last 20 standard reference toxicant (SRT) tests and the bench sheet s and statistical analyses of the SRT tests that correspond to these facility tests. See Appendix D for test organism receipts.

*NOTE: In a 48-hour acute screening test of 100% effluent, mortality of <20% provides confidence that the effluent meets the acute toxici ty criterion of Florida’s Surface Water Quality Standards (Rule 62-302.500 (1)(a) 4, F.A.C.). Mortality betwe en 20-50% indicates low to moderate levels of toxicity, and further action may be required. Mortality of >50% indicates that the effluent fails to meet the minimum requirement to discharge to waters of the state (Rule 62- 4.244(3)(a), F.A.C.).



### Algal Growth Potential The effluent Algal Growth Potential (AGP) was 24.9 mg dry wt/L of the unicellular green alga, Pseudokirchneriella subcapitata , formerly known as Selenastrum capricornutum . Raschke and Shultz (1987) found that AGP values above 5.0 mg dry weight/L represent a “problem” threshold for fresh receiving waters, implying nutrient enric hment. The analytical chemistry suggests that the effluent is nitrogen-limited. The predicted AG P based on the effluent’s inorganic nitrogen concentration was 10.3 (±20%) mg dry wt/L (Table 1; Miller et al. 1978).

The salinity-adjusted effluent AGP was 3.0 U (U - Material was analyzed for but not detected; the reported value is the minimum detection limit ) mg dry wt/L of the saltwater species, Dunaliella tertiolecta. Raschke and Shultz found that AGP values above 10.0 mg dry weight/L represent a “problem” threshold for marine receiving waters, implying nutrient enrichment (personal communication, Marshall Fairclot h, et. al. FDEP, with Ron Raschke USEPA Region 4 1987). One potential reason for the lack of growth in the salin ity-adjusted sample is precipitation of enough orthophosphate by the added salts that the sa mple became extremely phosphorus-limited (Manahan 1994).

See Table 1 for AGP results

### 3 Chemistry Results Total residual chlorine (TRC) and total ammonia we re not detected in the bioassay sample in the laboratory. TRC was not detected in the effluent in the field. The total ammonia concentration in the sample collected and preserved for chemical analysis was 0.1 mg/L Y (Y – the laboratory analysis was from an unpreserved or improp erly preserved sample; the data may not be accurate) mg/L. Based on the pH, salinity, and te mperature of the effluent as collected, the calculated unionized ammonia concentration was ≤ 0.02 mg/L.

Aluminum, arsenic, copper, nickel and norflurazon were detected in the effluent at levels that comply with Class III marine water quality criteria (62-302.530, F.A.C.). Bromacil, chromium III and lead were detected between the laboratory method detection limits (MDL) and practical quantitation limits (PQL).

The effluent iron (430 μg/L) violated the Class III marine water criterion for iron ( ≤ 300 μg/L; 62- 302.530(39), F.A.C.).

See Table 2 for results of analytes detected in the effluent, and corresponding limits. See Appendix E for a complete list of chemical analyses performed.



### Conclusion The effluent sample collected from the EFF-1 NPDES sampling point for Outfall D-010 for this facility on July 21, 2008, was not acutely toxic to either test species during the 48-hour acute screening bioassays. The effluent AGP result exceeded the "problem” threshold for fresh receiving waters. The AGP in the salinity-adjus ted effluent was less than the method detection limit. Effluent iron violated the Class III marine wate r criterion for iron. T he measured constituents of other effluent water quality samples did not exceed any applicable water quality standards or violate any permit conditions.



### Literature Cited Miller, W. E., T. E. Maloney, and J. C. Greene. 1978. The Selenastrum capricornutum Printz algal assay bottle test. EPA-600/9-78-018. U. S. EPA, Cincinnati, Ohio. 126 p.

Manahan, S. E. 1994. Environmental Chemistry, 6 th edition. Lewis Publishers, USA. 811 pp

Raschke, R. L. and D. A. Schultz. 1987. The use of the algal growth potential test for data assessment. J. Wat. Poll. Cont. Fed. 59(4): 222-227.

### 4 Florida Power and Light Company - Fort Myers Plant

Inor g anic Total Sample N:P ratio N:P ratio Efluent 24.9 10.3 ± 2. 1 74.9 ± 15.0 1.3 6.6 Table 1a. Measured and predicted algal growth potential (AGP; mg dry weight/L) for total soluble inorganic nitrogen (TSIN) a nd total nitrogen (TN) limit ation of the fr eshwater species Pseudokirchneriella subcapitata, and ratios of nitrogen to phosphorus for samples collected from the Florida Power and Light Company - Fort Myers Plant on July 21, 2008.

AGP (measured) Predicted AGP (TSIN) ± 20% Predicted AGP (TN) ± 20%

Florida Power and Light Company - Fort Myers Plant

Inor g anic Total N:P ratio N:P ratio Efluent 3.0 U 1.3 6.6 Table 1b. Measured algal growth potential (AGP; mg dry weight/L) of the saltwater species Dunaliella tertiolecta and ratios of nitrogen to phosphorus for samples collected from the Florida Power and Light Company - Fort Myers Plant on July 21, 2008.

AGP (measured)

U - Material analyzed for but not detected; value reported is the method detection limit

Salinity-adjusted Sample

### 5 Florida Power and Light Company - Fort Myers Plant NPDES# FL0001490 Class III Criteria Effluent Limits

Bromacil - - 0.59 IJ Norflurazon - - 1.4

Aluminum ≤ 1500 - 260 Arsenic ≤ 50 - 2.2 Cadmium ≤ 8.8 - 0.02 U Calcium (mg/L) - - 59.8 Chromium-III - - 0.68 I Copper ≤ 3.7 - 3.1 Iron ≤ 300 - 430 Lead ≤ 8.5 - 0.29 I Magnesium (mg/L) - - 13.4 Nickel ≤ 8.3 - 1.01 Selenium ≤ 71 - 0.5 U Silver ≤ 2.3 - 0.025 U Zinc ≤ 86 - 5 U

Ortho-phosphate - - 0.21 Y Total Phosphorus - - 0.3 Y Total Ammonia - - 0.1 Y Un-ionized Ammonia - - ≤ 0.02 c Nitrate and Nitrite - - 0.17 Y Total Kjeldahl Nitrogen - - 1.8 Y Organic Nitrogen - - 1.7 c Total Nitrogen - - 1.97 c

Dissolved Oxygen (mg/L) ≥ 4.0 s - 4.9 pH (SU) 6.5 - 8.5 s 6.5 - 8.5 s 7.5 Conductivity (μmhos/cm) - - 0.34 Temperature °C - Report 35.7 Sample Depth (m) - - 1 Total Residual Oxidants (mg/L) ≤ 0.01 s ≤ 0.01 s 0 Chlorophyll a (μg/L) - Corrected - - 8.6 AY Phaeophytin (μg/L) - - 3.1 AY CBOD, 5 day (mg/L), N - inhibited - - 1.5 AIY Oils and Greases (mg/L) ≤ 5.0 s - 1.8 I Flow (MGD) - - 670.3 a Hardness (mg CaCO 3 ) - - 204.50 c

Fecal Coliforms ≤ 400s # - 28 B

Value exceeds the Class III Water Q uality Criteria or permit limits a - Annual average c - Value is calculated s - Single sample A

- Value reported is the mean of two or more determinations B - Results based on colony counts outside the acceptance range I - The reported value is between the laboratory method dete ction limit and the laborator y practical quantitation limit J - Estimated value U - Material analyzed for but not detected; value reported is the method detection limit Y - The laboratory analysis was from an unpreserved or improperly preserved sample. The data may not be accurate. # - If 10 or fewer samples are taken within a month, the single sample limit is 400 CFU (62-302.530(6), F.A.C.) Table 2. Effluent limits, Class III Criteria for predominantly marine waters and chemical and microbiological data for samples collected from the Florida Power and Light Company - Fort Myers Plant on July 21, 2008.

General Physical and Chemical Parameters

Microbiology (Number of colony forming units/100ml) Efluent

Organic Constituents (μg/L)

Metals (μg/L unless otherwise noted)

Nutrients (mg/L)

### 6 Appendix A. Chain of Custody Form

### 7 Appendix B. Bench Sheets

### 8 Appendix B. (continued)

### 9 Appendix B. (continued)

### 10 Appendix B. (continued)

### 11 Appendix B. (continued)

### 12 Appendix B. (continued)

### 13 Appendix C. SRT Bench Sheet s and Statistical Analysis Florida Department of Environmental Protection Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - 48-hour Acute Toxicity Ceriodaphnia dubia (water flea) - Sodium Chloride (NaCl)

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit 01/16/07 2.22 2.19 0.17 1.85 2.52 02/13/07 2.39 2.31 0.17 1.97 2.54 03/13/07 1.92 2.18 0.18 1.82 2.55 04/03/07 2.37 2.20 0.18 1.83 2.57 05/08/07 1.78 2.18 0.21 1.77 2.59 05/29/07 1.69 2.06 0.23 1.70 2.60 06/06/07 2.28 2.16 0.22 1.71 2.60 07/03/07 1.99 2.14 0.22 1.70 2.58 07/31/07 1.94 2.14 0.22 1.71 2.58 09/04/07 1.95 2.13 0.22 1.70 2.57 10/02/07 2.04 2.11 0.21 1.68 2.53 10/30/07 2.35 2.11 0.21 1.68 2.54 12/04/07 2.13 2.09 0.20 1.67 2.51 01/22/08 1.92 2.08 0.20 1.66 2.50 02/05/08 2.25 2.08 0.20 1.68 2.48 03/04/08 2.15 2.08 0.20 1.68 2.49 04/01/08 2.50 2.11 0.22 1.68 2.55 05/06/08 2.19 2.11 0.22 1.68 2.55 06/03/08 1.91 2.10 0.22 1.66 2.54 07/08/08 2.21 2.11 0.22 1.67 2.55

Means are of the last 20 SRT tests. Lower Control Limit = mean - 2 * Std Dev CV = 10.35 Upper Control Limit = mean + 2 * Std Dev EPA reference 75th quartile CV = 29%



### Standard Reference Toxicant Data Ceriodaphnia dubia 48 hour LC 50

1.50 2.00 2.50 3.0001/16/0702/13/0703/13/0704/03/0705/08/0705/29/0706/06/0707/03/0707/31/0709/04/0710/02/0710/30/0712/04/0701/22/0802/05/0803/04/0804/01/0805/06/0806/03/0807/08/08



### Test Dateg/L NaCl

LC50 Mean LC50 Lower Control Limit Upper Control Limit

### 14 Appendix C. (continued) Florida Department of Environmental Protection Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - 48-hour Acute Toxicity C

y

prinella leedsi ( bannerfin shiner ) - Sodium Chloride ( NaCl )

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit 01/16/07 8.47 8.04 0.47 7.11 8.98 02/14/07 8.92 8.70 0.32 7.12 9.10 03/14/07 7.75 8.38 0.59 7.16 9.09 04/03/07 8.50 8.41 0.49 7.19 9.12 05/08/07 9.07 8.22 0.52 7.18 9.25 05/30/07 8.85 8.59 0.48 7.23 9.32 07/03/07 8.67 8.33 0.50 7.34 9.32 07/10/07 8.52 8.36 0.49 7.38 9.34 08/01/07 7.7 8.31 0.51 7.29 9.34 09/05/07 9.06 8.34 0.54 7.26 9.42 10/02/07 8.55 8.43 0.47 7.45 9.35 10/30/07 8.72 8.43 0.47 7.48 9.37 12/04/07 7.71 8.36 0.47 7.41 9.31 01/08/08 8.7 8.35 0.47 7.41 9.30 02/05/08 8.92 8.42 0.46 7.50 9.33 03/04/08 7.89 8.42 0.46 7.50 9.33 04/01/08 8.24 8.42 0.45 7.51 9.33 05/15/08 8.1 8.39 0.51 7.36 9.41 06/03/08 8.21 8.37 0.51 7.34 9.40 07/08/08 8.04 8.33 0.50 7.32 9.33

Means are of the last 20 SRT tests. Lower Confidence Limit = mean - 2 * Std Dev CV = 6.03 Upper Confidence Limit = mean + 2 * Std Dev EPA reference 75th quartile CV = 19%



### Standard Reference Toxicant Data Cyprinella leedsi 48-hour LC 50

4 6 8 1001/16/0702/14/0703/14/0704/03/0705/08/0705/30/0707/03/0707/10/0708/01/0709/05/0710/02/0710/30/0712/04/0701/08/0802/05/0803/04/0804/01/0805/15/0806/03/0807/08/08



### Test Dateg/L NaCl

LC50 Mean LC50 Lower Control Limit Upper Control Limit

### 15 Appendix C. (continued)

### 16 Appendix C. (continued)

### 17 Appendix C. (continued)

### 18 Appendix C. (continued)

### 19 Appendix C. (continued)



### Ceriodaphnia dubia Acute Standard Reference Toxicant Test

DATE: 7-8-08 DURATI ON: 48 hr TOXICANT : NaCl SPECIES: Ceriodaphnia dubia

RAW DATA: Concentration Number Mortalities (g/L) Exposed .00 20 0 1.50 20 0 2.00 20 1 2.50 20 20 3.00 20 20 4.00 20 20

SPEARMAN-KARBER TRIM: .00%

SPEARMAN-KARBER ESTIMATES: LC50: 2.21 95% LO WER CONFIDENCE: 2.15 95% UPPE R CONFIDENCE: 2.26

_

___________________ ______________________ ________________
Cyprinella leedsi Acute Standard Refe rence Toxicant Test

DATE: 7-8-08 DURATI ON: 48 hr TOXICANT : NaCl SPECIES: Cyprinella leedsi

RAW DATA: Concentration Number Mortalities (g/L) Exposed 0.00 20 0 6.00 20 2 7.00 20 3 8.00 20 9 9.00 20 16 10.00 20 20

SPEARMAN-KARBER TRIM: 0.00%

SPEARMAN-KARBER ESTIMATES: LC50: 8.04 95% LO WER CONFIDENCE: 7.64 95% UPPE R CONFIDENCE: 8.47

### 20 Appendix D. Test Organism Receipt(s)

### 21 Appendix D. (continued)22Appendix E. Chemical Analyses performed on the effluent from the Florida Power and Light-Fort Myers PlantOutfall D-010, sampled on July 21, 2008.Date Sampled Field ID Analysis Component Result Remark Units MDL PQL7/21/2008 11:30 Efluent AGP/LN Algal Growth Potential 24.9 mg DryWt/L 0.3 0.97/21/2008 11:30 Efluent AGP/LN/SA Algal Growth Potential Salinity-adjusted 3 U mg DryWt/L 3 37/21/2008 11:30 Efluent Chlorophyll/Grain Size/BOD Chlorophyll-a, Corrected 8.6 AY ug/L 0.55 1.77/21/2008 11:30 Efluent Chlorophyll/Grain Size/BOD Biochemical Oxygen Demand-5 Day,N-Inhib 1.5 AIY mg/L 0.2 27/21/2008 11:30 Efluent Chlorophyll/Grain Size/BOD Phaeophytin-a 3.1 AY ug/L 0.24 0.717/21/2008 11:30 Efluent Overflow Fecal Coliforms-Membrane Filter 28 B #/100 mL7/21/2008 11:30 Efluent Toxicology Bioassay-Acute-Screen-FW-C.dubia, LC50 100 L LC507/21/2008 11:30 Efluent Toxicology Bioassay-Acute-Screen-FW-Fish, LC50 100 L LC507/21/2008 11:30 Efluent Metals Chromium 0.68 I ug/L 0.5 27/21/2008 11:30 Efluent Metals Aluminum 260 ug/L 60 2407/21/2008 11:30 Efluent Metals Calcium 59.8 mg/L 0.075 0.37/21/2008 11:30 Efluent Metals Magnesium 13.4 mg/L 0.04 0.167/21/2008 11:30 Efluent Metals Arsenic 2.2 ug/L 0.5 27/21/2008 11:30 Efluent Metals Zinc 5 U ug/L 5 207/21/2008 11:30 Efluent Metals Silver 0.025 U ug/L 0.025 0.17/21/2008 11:30 Efluent Metals Copper 3.1 ug/L 0.5 27/21/2008 11:30 Efluent Metals Iron 430 ug/L 30 1207/21/2008 11:30 Efluent Metals Cadmium 0.02 U ug/L 0.02 0.087/21/2008 11:30 Efluent Metals Selenium 0.5 U ug/L 0.5 27/21/2008 11:30 Efluent Metals Antimony 0.5 U ug/L 0.5 27/21/2008 11:30 Efluent Metals Lead 0.29 I ug/L 0.2 0.87/21/2008 11:30 Efluent Metals Nickel 1.01 ug/L 0.25 17/21/2008 11:30 Efluent Metals Beryllium 0.025 U ug/L 0.025 0.17/21/2008 11:30 Efluent Nutrients Total-P 0.3 Y mg P/L 0.02 0.067/21/2008 11:30 Efluent Nutrients Kjeldahl Nitrogen 1.8 Y mg N/L 0.08 0.27/21/2008 11:30 Efluent Nutrients O-Phosphate-P 0.21 Y mg P/L 0.004 0.017/21/2008 11:30 Efluent Nutrients NO2NO3-N 0.17 Y mg N/L 0.004 0.017/21/2008 11:30 Efluent Nutrients Ammonia-N 0.1 Y mg N/L 0.01 0.027/21/2008 11:30 Efluent Overflow Oil and Grease 1.8 I mg/L 1.4 57/21/2008 11:30 Efluent Pesticides Ametryn 0.048 U ug/L 0.048 0.197/21/2008 11:30 Efluent Pesticides Atrazine 0.048 U ug/L 0.048 0.197/21/2008 11:30 Efluent Pesticides Metalaxyl 0.24 U ug/L 0.24 0.967/21/2008 11:30 Efluent Pesticides Norflurazon 1.4 ug/L 0.14 0.5623Appendix E. (continued)Date Sampled Field ID Analysis Component Result Remark Units MDL PQL7/21/2008 11:30 Efluent Pesticides Ethion 0.048 U ug/L 0.048 0.197/21/2008 11:30 Efluent Pesticides Malathion 0.14 U ug/L 0.14 0.567/21/2008 11:30 Efluent Pesticides Phorate 0.048 UJ ug/L 0.048 0.197/21/2008 11:30 Efluent Pesticides Alachlor 0.58 U ug/L 0.58 2.37/21/2008 11:30 Efluent Pesticides Chlorpyrifos Ethyl 0.048 U ug/L 0.048 0.197/21/2008 11:30 Efluent Pesticides Hexazinone 0.096 U ug/L 0.096 0.387/21/2008 11:30 Efluent Pesticides Azinphos Methyl 0.19 U ug/L 0.19 0.767/21/2008 11:30 Efluent Pesticides Butylate 0.19 U ug/L 0.19 0.767/21/2008 11:30 Efluent Pesticides Chlorpyrifos Methyl 0.096 U ug/L 0.096 0.387/21/2008 11:30 Efluent Pesticides Mevinphos 0.19 UJ ug/L 0.19 0.767/21/2008 11:30 Efluent Pesticides Naled 0.77 U ug/L 0.77 3.17/21/2008 11:30 Efluent Pesticides Diazinon 0.048 U ug/L 0.048 0.197/21/2008 11:30 Efluent Pesticides Ethoprop 0.096 UJ ug/L 0.096 0.387/21/2008 11:30 Efluent Pesticides Simazine 0.048 U ug/L 0.048 0.197/21/2008 11:30 Efluent Pesticides Bromacil 0.59 IJ ug/L 0.19 0.767/21/2008 11:30 Efluent Pesticides Prometryn 0.14 U ug/L 0.14 0.567/21/2008 11:30 Efluent Pesticides Fenamiphos 0.19 U ug/L 0.19 0.767/21/2008 11:30 Efluent Pesticides Fonofos 0.096 U ug/L 0.096 0.387/21/2008 11:30 Efluent Pesticides Metolachlor 0.48 U ug/L 0.48 1.97/21/2008 11:30 Efluent Pesticides Metribuzin 0.096 U ug/L 0.096 0.387/21/2008 11:30 Efluent Pesticides Parathion Ethyl 0.14 U ug/L 0.14 0.567/21/2008 11:30 Efluent Pesticides Parathion Methyl 0.096 U ug/L 0.096 0.387/21/2008 11:30 Efluent Priority Organic Pollutants Benzo(a)pyrene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants Indeno(1,2,3-cd)pyrene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants Isophorone 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants Phenol 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants 4-Chloro-3-methylphenol 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants Benzo(b)fluoranthene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic PollutantsBis(2-chloroisopropyl)ether 2.9 U ug/L 2.9 127/21/2008 11:30 Efluent Priority Organic Pollutants Diethyl phthalate 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants Endrin aldehyde 3.8 U ug/L 3.8 157/21/2008 11:30 Efluent Priority Organic Pollutants Heptachlor 1.4 U ug/L 1.4 5.87/21/2008 11:30 Efluent Priority Organic Pollutants Phenanthrene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants 2-Nitrophenol 0.96 U ug/L 0.96 3.824Appendix E. (continued)Date Sampled Field ID Analysis Component Result Remark Units MDL PQL7/21/2008 11:30 Efluent Priority Organic Pollutants 4,4'-DDT 1.4 U ug/L 1.4 5.87/21/2008 11:30 Efluent Priority Organic Pollutants 4-Chlorophenyl phenyl ether 1.9 U ug/L 1.9 7.77/21/2008 11:30 Efluent Priority Organic Pollutants Benzo(g,h,i)perylene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic PollutantsBis(2-chloroethoxy)methane 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants Dibenzo(a,h)anthracene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants Endosulfan II 3.8 U ug/L 3.8 157/21/2008 11:30 Efluent Priority Organic Pollutants Endrin 1.4 U ug/L 1.4 5.87/21/2008 11:30 Efluent Priority Organic Pollutants Naphthalene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants Pentachlorophenol 2.9 U ug/L 2.9 127/21/2008 11:30 Efluent Priority Organic Pollutants 1,2,4-Trichlorobenzene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants 2,4,6-Trichlorophenol 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants 2,4-Dimethylphenol 9.6 U ug/L 9.6 387/21/2008 11:30 Efluent Priority Organic Pollutants 2-Chlorophenol 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants 2-Methyl-4,6-dinitrophenol 2.9 U ug/L 2.9 127/21/2008 11:30 Efluent Priority Organic Pollutants Aldrin 1.4 U ug/L 1.4 5.87/21/2008 11:30 Efluent Priority Organic Pollutants Dieldrin 1.4 U ug/L 1.4 5.87/21/2008 11:30 Efluent Priority Organic Pollutants Fluorene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants Heptachlor epoxide 1.4 U ug/L 1.4 5.87/21/2008 11:30 Efluent Priority Organic PollutantsN-Nitrosodi-n-propylamine 1.9 U ug/L 1.9 7.77/21/2008 11:30 Efluent Priority Organic Pollutants Acenaphthylene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants Benzo(a)anthracene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants Benzo(k)fluoranthene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic PollutantsBis(2-chloroethyl)ether 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants Chrysene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants Hexachlorocyclopentadiene 2.9 U ug/L 2.9 127/21/2008 11:30 Efluent Priority Organic Pollutants Hexachloroethane 2.9 U ug/L 2.9 127/21/2008 11:30 Efluent Priority Organic Pollutants beta-BHC 1.4 U ug/L 1.4 5.87/21/2008 11:30 Efluent Priority Organic Pollutants Anthracene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants Benzidine 96 U ug/L 96 3807/21/2008 11:30 Efluent Priority Organic Pollutants Bis(2-ethylhexyl)phthalate 14 U ug/L 14 587/21/2008 11:30 Efluent Priority Organic Pollutants Di-n-butyl phthalate 4.8 U ug/L 4.8 197/21/2008 11:30 Efluent Priority Organic Pollutants Di-n-octyl phthalate 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants Hexachlorobenzene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants Hexachlorobutadiene 2.9 U ug/L 2.9 1225Appendix E. (continued)Date Sampled Field ID Analysis Component Result Remark Units MDL PQL7/21/2008 11:30 Efluent Priority Organic Pollutants Nitrobenzene 1.9 U ug/L 1.9 7.77/21/2008 11:30 Efluent Priority Organic Pollutants Pyrene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants 2,4-Dinitrotoluene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants 4-Bromophenyl phenyl ether 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants Dimethyl phthalate 9.6 UJ ug/L 9.6 387/21/2008 11:30 Efluent Priority Organic Pollutants Endosulfan sulfate 1.4 U ug/L 1.4 5.87/21/2008 11:30 Efluent Priority Organic Pollutants alpha-BHC 1.4 U ug/L 1.4 5.87/21/2008 11:30 Efluent Priority Organic Pollutants delta-BHC 1.4 U ug/L 1.4 5.87/21/2008 11:30 Efluent Priority Organic Pollutants gamma-BHC 1.4 U ug/L 1.4 5.87/21/2008 11:30 Efluent Priority Organic Pollutants 2,4-Dichlorophenol 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants 4-Nitrophenol 14 U ug/L 14 587/21/2008 11:30 Efluent Priority Organic Pollutants Acenaphthene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants Butyl benzyl phthalate 4.8 U ug/L 4.8 197/21/2008 11:30 Efluent Priority Organic Pollutants Endosulfan I 3.8 U ug/L 3.8 157/21/2008 11:30 Efluent Priority Organic Pollutants Fluoranthene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants N-Nitrosodimethylamine 1.9 U ug/L 1.9 7.77/21/2008 11:30 Efluent Priority Organic Pollutants 2,4-Dinitrophenol 14 U ug/L 14 587/21/2008 11:30 Efluent Priority Organic Pollutants 2,6-Dinitrotoluene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants 2-Chloronaphthalene 0.96 U ug/L 0.96 3.87/21/2008 11:30 Efluent Priority Organic Pollutants 3,3'-Dichlorobenzidine 38 U ug/L 38 1507/21/2008 11:30 Efluent Priority Organic Pollutants 4,4'-DDD 1.4 U ug/L 1.4 5.87/21/2008 11:30 Efluent Priority Organic Pollutants 4,4'-DDE 1.4 U ug/L 1.4 5.87/21/2008 11:30 Efluent Field Parameter Sample Depth 1 m7/21/2008 11:30 Efluent Field Parameter pH 7.527/21/2008 11:30 Efluent Field Parameter Specific Conductance 0.34 umhos/cm7/21/2008 11:30 Efluent Field Parameter Temperature 35.74 C7/21/2008 11:30 Efluent Field Parameter Dissolved Oxygen 4.9 mg/L

### 26 Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 1 4 9 0 11 12 0 8 0 7 2 1 18 B 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 1 4 9 0 11 12 0 8 0 7 2 1 18 X 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 1 4 9 2 11 12 0 8 0 7 2 1 18 S 19 S 20 2

66 

## Biological Analyses of Florida Power and Light-Fort Myers Plant effluent sampled on July 21 , 2008. NPDES #FL0001490.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks 

## The Bioassay of Florida Power and Light-Fort Myers Plant effluent sampled on July 21, 20 08. NPDES #FL0001490.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks



## The Priority Pollutants Analysis of Florida Power and Light-Fort Myers Plant effluent sampled on July 21, 2008. NPDES #FL0001490.