SRC TR-10-120

HPV4 CHEMICAL DATA ADEQUACY REVIEW

1H-Benzotriazole, 4(or 5)-methyl-, sodium salt, CAS No. 64665-57-2

Prepared by:

Justine Von Runnen, Chris Janson, and Joan D. Garey

Chemical, Biological and Environmental Center

SRC, Inc.

7502 Round Pond Road

North Syracuse, NY 13212

Contract No. EP-W-09-027

Task FG007.0.009.002

Submitted to:

OPPT/RAD/HPVCB (7403M)

U.S. Environmental Protection Agency

1200 Pennsylvania Avenue

Washington, D.C. 20460

Elizabeth Margosches, Alternate Project Officer

Meena Sonawane, Work Assignment Manager

David Brooks, Alternate Work Assignment Manager

July 9, 2010
HPV4 Chemical Data Adequacy Review

1H-Benzotriazole, 4(or 5)-methyl-, sodium salt, CAS No. 64665-57-2

July 2010

Submitted to:

High Production Volume Chemicals Branch 

Risk Assessment Division 

Office of Pollution Prevention and Toxics 

Environmental Protection Agency 

WAM: Meena Sonawane

Alternate WAM: David Brooks

1200 Pennsylvania Avenue, NW 

Washington, DC 20460-0001

Prepared By:

SRC, Inc. 

Environmental Science Center 

2451 Crystal Drive, Suite 804 

Arlington, VA 22202
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A. PREPARATION OF THE DATA ADEQUACY ASSESSMENTS

A-1. Source material used for assessment

For this unsponsored chemical, a comprehensive literature search was performed to 

identify available toxicity data for the following SIDS endpoints:

Acute toxicity to fish

Acute toxicity to Daphnia

Toxicity to aquatic plants

Acute (oral, dermal, inhalation) toxicity

Repeated-dose toxicity

Reproductive toxicity

Developmental toxicity

Gene mutation

Chromosomal aberrations

Studies relevant to non-SIDS endpoints (e.g., eye and skin irritation; neurotoxicity) conducted 

via oral, inhalation, or dermal routes were also identified through literature searching.

The following sources were searched for toxicity data:

PubMed - Bibliographic database that contains references to journal articles in the life 

sciences with a concentration on biomedicine

TOXNET -National Library of Medicine databases on toxicology, hazardous chemicals, 

environmental health, and toxic releases; includes: 

• TOXLINE with BIOSIS and NTIS;

• Development and Reproductive Toxicology File (DART);

• Chemical Carcinogenesis Research Information System (CCRIS) -

Carcinogenicity and mutagenicity test results for over 8000 

• chemicals);

• Hazardous Substances Data Bank (HSDB) - Comprehensive, peerreviewed toxicology data for about 5000 chemicals;

• Genetic Toxicology Data Bank (GENE-TOX) - Peer-reviewed genetic 

toxicology test data for over 3000 chemicals.

TSCATS - Unpublished technical reports submitted by industry to the EPA under the 

Toxic Substances Control Act]

Registry of toxic effects of chemical substances (RTECs)

Environmental Fate Database (EFDB)

ECOTOX -EPA’s peer-reviewed ecotoxicology database
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EPA’s Integrated Risk Information System (IRIS) website

Agency for Toxic Substances and Disease Registry (ATSDR) website

National Toxicology Program (NTP) website

International Program on Chemical Safety Concise International Chemical Assessment 

Documents (IPCS INCHEM or CICADs)

European Chemical Substances Information System (ESIS) - source of IUCLID Datasets

Any available IUCLID Datasets found through the ESIS website and robust summaries 

submitted to EPA by former sponsors on the assigned chemicals were retrieved. Original 

sources were obtained for any relevant toxicity data found for the assigned chemicals. 

While IUCLID Datasets sometimes contained studies of interest, certain study 

descriptions contained insufficient detail to assess adequacy. In these cases, the primary source 

of the study, if available and accessible, was obtained and reviewed.

A-2. Evaluating data adequacy

Using standard OECD testing guidelines and professional judgment as guides, available 

toxicity studies for the unsponsored chemical were reviewed for adequacy. Summaries were 

prepared for all relevant data found, even if little or no study detail was available. Each study 

was summarized according to a standard format and assigned a Klimisch reliability code; all 

study summaries for the chemical were combined into a single summary document. For studies 

already assigned Klimisch codes (e.g., studies summarized in IUCLID datasets), an explanation 

was provided if the reviewer’s assessment of data adequacy differed from the pre-assigned 

Klimisch code. A table summarizing data availability/adequacy for each SIDS endpoint is 

included at the end of the summary document. 

B. SPECIFIC INFORMATION ON THE AVAILABLE TOXICITY DATA

The available data for each of the SIDS toxicity endpoints are evaluated on the following 

pages. Studies for a common endpoint are grouped together in the document and an endpoint 

adequacy assessment is provided near the top of the first page of the summaries for that 

endpoint. If the data available for the endpoint is determined to be inadequate, a basis for that 

conclusion is supplied. 
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B-1. TOXICITY TO AQUATIC VERTEBRATES

Summary of endpoint: Adequate

Study 1: Acute toxicity to bluegill sunfish

Title: Acute Toxicity to Fish 

This study is: Adequate Not Adequate

TEST SUBSTANCE 

Identity: 1H-Benzotriazole, 4(or 5)-methyl-, sodium salt, CAS No. 64665-57-2

Remarks: 50% sodium tolyltriazole in water 

METHOD

Method/guideline followed: Not stated

Test type: Static

GLP compliant?: Yes

Year study was performed: 1984

Test species: Bluegill sunfish (Lepomis macrochirus)

Analytical monitoring: Not stated

Exposure duration: 96 hours

Concentrations tested: 0, 15, 23, 34, 51, 77, 115 and 173 ppm 

Test method/conditions remarks: Bluegill sunfish (initial bw = 5.0g) were acclimated for at 

least 5 days. During the experiment, the fish were not fed. On Day 0, 10 fish were introduced 

into test chambers containing nominal concentrations of 15, 23, 34, 51, 77, 115, and 173 ppm 

test substance. Fish were observed daily for mortality and signs of intoxication. 

Test Condition: Water temperature and dissolved Oxygen measured daily; pH measured at 

beginning and end of study; hardness and alkalinity measured at beginning of study. 

RESULTS

Endpoint value(s): The 96-hour LC50 value for bluegill sunfish was > 173 ppm. The NOEC 

was 34 ppm. 
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Results remarks:

Photoperiod = 16 hours light/day 

Temperature: 15 - 18 degree C 

pH: 7.1 - 7.8 

Dissolved Oxygen: 5.4 - 8.4 mg/l 

Hardness: 143 - 147 mg/l 

Alkalinity: 54 - 55 mg/l 

Aeration: none 

Conc #fish Mortality/ signs of intoxication

24h 48h 72h 96h 

Control 20 0/0 0/0 0/0 0/0

15 ppm 10 0/0 0/0 0/0 0/0

23 ppm 10 0/0 0/0 0/0 0/0

34 ppm 10 0/0 0/0 0/0 0/0

51 ppm 10 0/7 0/4 0/3 0/0

77 ppm 10 1/9 1/9 1/9 1/9

115 ppm 10 0/10 0/10 0/10 0/10

173 ppm 10 0/10 0/10 0/10 0/10

Clinical signs were present at concentrations from 53 to 173. In general, the fish were either at 

the surface or on the bottom and exhibited almost total inactivity, but were hyperreactive to 

stimuli. Fish in the 51ppm group recovered by 96 hours, but none did in the higher 

concentrations. One fish died in the 77ppm group, which appears to be an unusual sensitivity 

since there were no deaths at the two higher concentrations. 

CONCLUSIONS

STUDY RELIABILITY

[1] Reliable without restrictions. 

Reliability remarks: GLP study, Meets generally accepted scientific method and is described in 

sufficient detail. 

Flag: Critical study for SIDS endpoint 

REFERENCE

SOCMA, Benzotriazole Coalition. IUCLID Data Set ID: 64665-57-2 (12/6/2001). Study No.

84-066-02. Mobay Chemical Corporation. August 3, 1984.
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Study 2: Acute toxicity to rainbow trout

Title: Acute Toxicity to Fish 

This study is: Adequate Not Adequate

TEST SUBSTANCE 

Identity: 1H-Benzotriazole, 4(or 5)-methyl-, sodium salt, CAS No. 64665-57-2

Remarks: 50% sodium tolyltriazole in water 

METHOD

Method/guideline followed: Not stated

Test type: Not stated

GLP compliant?: Yes

Year study was performed: 1984

Test species: Rainbow trout (Salmo gairdneri)

Analytical monitoring: Not stated

Exposure duration: 96 hours

Concentrations tested: 0, 16.6, 19.9, 23.2, 27.4, 32.5, 38.4, and 53.8 ppm

Test method/conditions remarks: Rainbow trout (initial bw of 1.21 - 1.35g) were acclimated 

for at least 5 days. During the experiment, the fish were not fed and the water was not aerated. 

On Day 0, 10 fish were introduced into test chambers containing nominal concentrations of 16.6, 

19.9, 23.2, 27.4, 32.5, 38.4, and 53.8 ppm test substance. Fish were observed daily for mortality

and signs of intoxication. LC50 and 95% confidence limits were calculated according to Weil 

CS. 1952. Biometrics. 8:249.

Test Condition: Water temperature and dissolved Oxygen measured daily; pH measured at 

beginning and end of study; hardness and alkalinity measured at beginning of study.

RESULTS

Endpoint value(s): The 24-, 48-, 72-, and 96-hour LC50 values for rainbow trout were > 53.8, 

33 (31-35), 31 (29-32), and 25 (24-26) ppm, respectively.
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Results remarks:

Photoperiod = 16 hours light/day

Temperature: 9.0 - 12.3 degree C

pH: 7.1 - 8.6

Dissolved Oxygen: 3.4 - 18.8 mg/l

Hardness: 151 - 163 mg/l

Alkalinity: 56 -70 mg/l

Conc #fish Mortality/ signs of intoxication

24h 48h 72h 96h 

Control 20 0/0 0/0 0/0 0/0

16.6 ppm 10 0/0 0/0 0/0 0/0

19.9 ppm 10 0/0 0/1 0/4 0/4*

23.2 ppm 10 0/0 0/10 1/9* 1/9*

27.4 ppm 10 0/2 0/10* 0/10* 10/-*

32.5 ppm 10 0/10 9/1* 9/1* 9/1*

38.4 ppm 10 0/9 5/5* 9/1* 10/-

53.8 ppm 10 0/10 10/-* 10/- 10/-

* mortality values used to calculate the LC50.

CONCLUSIONS

STUDY RELIABILITY

[1] Reliable without restrictions. 

Reliability remarks: GLP study, Meets generally accepted scientific method and is described in 

sufficient detail. 

Flag: Critical study for SIDS endpoint 

REFERENCE

SOCMA, Benzotriazole Coalition. IUCLID Data Set ID: 64665-57-2 (12/6/2001). Study No. 

84-066-01. Mobay Chemical Corporation. June 15, 1984.
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Study 3: Acute toxicity to zebrafish

Title: Acute Toxicity to Fish 

This study is: Adequate Not Adequate

This study was considered inadequate due to missing critical study information.

TEST SUBSTANCE 

Identity: 1H-Benzotriazole, 4(or 5)-methyl-, sodium salt, CAS No. 64665-57-2

Remarks: 50% sodium tolyltriazole in water 

METHOD

Method/guideline followed: Not stated

Test type: Not stated

GLP compliant?: Not stated

Year study was performed: Not stated

Test species: Zebrafish (Brachydanio rerio)

Analytical monitoring: Not stated

Exposure duration: 96 hours

Concentrations tested: Not stated

Test method/conditions remarks: Not stated

RESULTS

Endpoint value(s): The 96-hour LC0 and LC50 values for zebra fish were 100 and 122 mg/L, 

respectively.

CONCLUSIONS

STUDY RELIABILITY

[4] Not assignable
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REFERENCE

Safety Data Sheet Bayer AG, 09.02.1999
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Study 4: Acute toxicity to bluegill sunfish

Title: Acute Toxicity to Fish 

This study is: Adequate Not Adequate

This study was considered inadequate due to missing critical study information.

TEST SUBSTANCE 

Identity: 1H-Benzotriazole, 4(or 5)-methyl-, sodium salt, CAS No. 64665-57-2

Remarks: 50% sodium tolyltriazole in water 

METHOD

Method/guideline followed: Not stated

Test type: Not stated

GLP compliant?: Not stated

Year study was performed: Not stated

Test species: Bluegill sunfish (Lepomis macrochirus)

Analytical monitoring: Not stated

Exposure duration: 96 hours

Concentrations tested: Not stated

Test method/conditions remarks: Not stated

RESULTS

Endpoint value: The 96-hour LC50 value for bluegill sunfish was 191.2 mg/L.

CONCLUSIONS

STUDY RELIABILITY

[4] Not assignable
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REFERENCE

PMC Specialties Group, Inc., MSDS 01/29/97
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Study 5: Acute toxicity to rainbow trout

Title: Acute Toxicity to Fish 

This study is: Adequate Not Adequate

This study was considered inadequate due to missing critical study information.

TEST SUBSTANCE 

Identity: 1H-Benzotriazole, 4(or 5)-methyl-, sodium salt, CAS No. 64665-57-2

Remarks: 50% sodium tolyltriazole in water 

METHOD

Method/guideline followed: Not stated

Test type: Not stated

GLP compliant?: Not stated

Year study was performed: Not stated

Test species: Rainbow trout (Salmo gairdneri)

Analytical monitoring: Not stated

Exposure duration: 96 hours

Concentrations tested: Not stated

Test method/conditions remarks: Not stated

RESULTS

Endpoint value(s): The 96-hour LC50 value for rainbow trout was 23.7 mg/L.

CONCLUSIONS

STUDY RELIABILITY

[4] Not assignable
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REFERENCE

PMC Specialties Group, Inc., MSDS 01/29/97
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B-2. TOXICITY TO AQUATIC INVERTEBRATES

Summary of endpoint: Adequate 

Study 1: Acute toxicity to Daphnia

Title: Acute toxicity to Daphnia 

This study is: Adequate Not Adequate

TEST SUBSTANCE 

Identity: 1H-Benzotriazole, 4(or 5)-methyl-, sodium salt, CAS No. 64665-57-2

Remarks: 50% aqueous solution of sodium tolyltriazole 

METHOD

Method/guideline followed: Other: according to TSCA Public Law 94-469 (1976) and USEPA 

Registration of Pesticides guidelines Fed. Reg. 07/10/78:29696-29741(1978)

Test type: Static

GLP compliant?: Yes

Year study was performed: 1978

Test species: Daphnia magna (Crustacea)

Analytical monitoring: Not stated

Exposure duration: 48-hours

Concentrations tested: 0, 100, 180, 320, 560 and 1000 mg/L

Test method/conditions remarks: Water temperature, pH, and dissolved Oxygen measured at 

beginning and end of study; hardness and alkalinity measured at beginning of study.

RESULTS

Endpoint value(s): The 24- and 48-hour LC50 values for daphnids were 440 (320-560) and 280 

(180-560) mg/L. The NOEC was 100 mg/L.

Results remarks:

Photoperiod = 16 hours light/day 

Temperature: 20 degree C 
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pH: 8.3 - 8.8 

Dissolved Oxygen: 7.4 - 8.1 mg/l 

Hardness: 225 - 275 ppm 

Alkalinity: 325 - 375 ppm

Conc Mortality/ total # Daphnia

24h 48h

Control 0/20 0/20

100 mg/l 0/20 0/20

180 mg/l 0/20 0/20

320 mg/l 0/20 14/20

560 mg/l 19/20 20/20

1000 mg/l 20/20 20/20

Clinical signs present at 180 mg/l were erratic behavior; at 320 mg/l Daphnia were either at the 

surface or on the bottom; and at 560 mg/l one exhibited loss of equilibrium.

CONCLUSIONS

STUDY RELIABILITY

[1] Reliable without restrictions.

Reliability remarks: GLP, National standards method 

Flag: Critical study for SIDS endpoint

REFERENCE

Analytical Bio-Chemistry Laboratories, Inc. Report #32028. August 24, 1984
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Study 2: Acute toxicity to Daphnia

Title: Acute toxicity to Daphnia

This study is: Adequate Not Adequate

This study was considered inadequate due to missing critical study information.

TEST SUBSTANCE 

Identity: 1H-Benzotriazole, 4(or 5)-methyl-, sodium salt, CAS No. 64665-57-2

METHOD

Method/guideline followed: Not stated

Test type: Not stated

GLP compliant?: Not stated

Year study was performed: Not stated

Test species: Daphnia magna (Crustacea)

Analytical monitoring: Not stated

Exposure duration: 48-hours

Concentrations tested: Not stated

Test method/conditions remarks: Not stated

RESULTS

Endpoint value: The 48-hour LC50 value for daphnids was 245.7 mg/L. 

Results remarks: Not stated

CONCLUSIONS

STUDY RELIABILITY

[4] Not assignable.
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REFERENCE

PMC Specialties Group, Inc., MSDS 01/29/97
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B-3. TOXICITY TO AQUATIC PLANTS

Summary of endpoint: Adequate 

Study 1: Toxicity to green algae

Title: Toxicity to green algae

This study is: Adequate Not Adequate

TEST SUBSTANCE 

Identity: 1H-Benzotriazole, 4(or 5)-methyl-, sodium salt, CAS No. 64665-57-2

Remarks: 50% aqueous solution of sodium tolyltriazole 

METHOD

Method/guideline followed: Not stated

Test type: Growth rate

GLP compliant?: Yes

Year study was performed: Not stated

Test species: Green algae (Selenastrum capricornutum)

Analytical monitoring: Not stated

Exposure duration: 96-hours

Concentrations tested: 0, 10, 30, 90, 270, and 810 mg/l (nominal concentrations) 

Test method/conditions remarks: Selenastrum capricornutum (2x10 e4 cells/ml) were 

exposed to test solutions of 0, 10, 30, 90, 270, and 810 mg/l (nominal concentrations) for four 

days and observed during a four day withdrawal period. Growth was monitored by cell counts 

and estimated biomass. 

Comparison of cell numbers and biomass during exposure and withdrawal were made using 

Duncan's multiple range test with alpha = 0.05. Dose-response analysis was done by probit 

method using log nominal concentrations. Biomass was estimated as: biomass (mg) = # cells x 

cell volume x 3.6 x 10 e-7. 

Test condition: The cultures were kept at 26-27 degree C with 400 +/-40 footcandles continuous 

cool-white fluorescent illumination and were continually oscillated on a platform at 100 rpm. 
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Each concentration was tested in triplicate.

RESULTS

Endpoint value(s): 

Growth EC50 (96hr) = 26.2 ppm (16.6- 36.7) 

Biomass EC50 (96hr) = 32.0 ppm (4.1 - 78.7) 

NOEC = 100 ppm 

Lowest algastatic concentration = 270 ppm 

No level tested was algacidal. 

CONCLUSIONS

STUDY RELIABILITY

[1] Reliable without restrictions.

Reliability remarks: GLP study, Meets generally accepted scientific method and is described in 

sufficient detail. 

Flag: Critical study for SIDS endpoint

REFERENCE

Study No. 84-060-02. Mobay Chemical Corporation. August 2, 1984.
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B-4. ACUTE TOXICITY

Summary of endpoint: Adequate

Study 1: Acute oral toxicity gavage study in rats

Title: Acute oral toxicity in Wistar rats

The study is: Adequate Not Adequate

TEST SUBSTANCE 

Identity: 1H-Benzotriazole, 4(or 5)-methyl-, sodium salt, CAS No. 64665-57-2

Remarks: 50% sodium tolyltriazole in water 

METHOD

Method/guideline followed: Not stated

Test type: LD50

GLP compliant?: No data 

Year study performed: 1983

Test species: Wistar rats

Number of animals/sex/dose: 5/sex/dose

Route of administration: Oral gavage

Vehicle: Not data

Doses/concentrations tested: 1.0, 1.3, 1.6, 1.8, 2.5, 2.8 ml/kg-bw 

Statistical methods used: Not stated

Test method/conditions remarks: 5 rats/sex/dose, 5 doses: 1.0, 1.3, 1.6, 1.8, 2.5, 2.8 ml/kg 

bw, given by gavage, observation time: 14 d, pathologic examination of rats that died during 

observation time and of the survivors after termination. 

RESULTS

Endpoint value: LD50 = 1.66 ml/kg-bw (ca. 1980 mg/kg-bw)
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Results remarks: Signs of intoxication: reduced general condition, prone position, rough fur, 

sedation. Pathologic examination: decedents: corrosion of the gastric mucous membranes; 

survivors: no findings. 

CONCLUSIONS

STUDY RELIABILITY

[2] Reliable with restrictions

Reliability remarks: Meets generally accepted scientific method and is described in sufficient 

detail. 

Flag: Critical study for SIDS endpoint

REFERENCE

Bayer AG data, E. Löser: Untersuchungen zur akuten oralen Toxizität an männlichen und 

weiblichen Ratten, June 1983.
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Study 2: Acute oral toxicity gavage study in male rats

Title: Acute oral toxicity in the male rat

The study is: Adequate Not Adequate

This study was considered inadequate due to missing critical study information.

TEST SUBSTANCE 

Identity: 1H-Benzotriazole, 4(or 5)-methyl-, sodium salt, CAS No. 64665-57-2

Remarks: 50% sodium tolyltriazole in water

METHOD

Method/guideline followed: Not stated

Test type: LD50

GLP compliant?: Not stated

Year study performed: Not stated

Test species: Rat

Number of animals/sex/dose: Not stated/ male

Route of administration: Oral gavage

Vehicle: No data

Doses/concentrations tested: Not stated

Statistical methods used: Not stated

Test method/conditions remarks: Not stated

RESULTS

Endpoint value(s): LD50 = 920 mg/kg-bw (ca.)

Results remarks: Not stated
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CONCLUSIONS

STUDY RELIABILITY

[4] Not assignable.

REFERENCE

PMC Specialties Group, Inc., MSDS 01/29/97

Safety Data Sheet Bayer AG, 09.02.1999
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Study 3: Acute oral toxicity gavage study in female rats

Title: Acute oral toxicity in the female rat

The study is: Adequate Not Adequate

This study was considered inadequate due to missing critical study information.

TEST SUBSTANCE 

Identity: 1H-Benzotriazole, 4(or 5)-methyl-, sodium salt, CAS No. 64665-57-2

Remarks: 50% sodium tolyltriazole in water

METHOD

Method/guideline followed: Not stated

Test type: LD50

GLP compliant?: Not stated

Year study performed: Not stated

Test species: Rat

Number of animals/sex/dose: Not stated/ female

Route of administration: Oral gavage

Vehicle: No data

Doses/concentrations tested: Not stated

Statistical methods used: Not stated

Test method/conditions remarks: Not stated

RESULTS

Endpoint value(s): LD50 = 640 – 735 mg/kg-bw

Results remarks: Not stated
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CONCLUSIONS

STUDY RELIABILITY

[4] Not assignable.

REFERENCE

PMC Specialties Group, Inc., MSDS 01/29/97

Safety Data Sheet Bayer AG, 09.02.1999
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Study 4: Acute dermal toxicity in rabbits

Title: Acute dermal toxicity in the rabbit

The study is: Adequate Not Adequate

This study was considered inadequate due to missing critical study information.

TEST SUBSTANCE 

Identity: 1H-Benzotriazole, 4(or 5)-methyl-, sodium salt, CAS No. 64665-57-2

Remarks: 50% sodium tolyltriazole in water

METHOD

Method/guideline followed: Not stated

Test type: LD50

GLP compliant?: Not stated

Year study performed: Not stated

Test species: Rabbit

Number of animals/sex/dose: Not stated

Route of administration: Dermal

Vehicle: No data

Doses/concentrations tested: Not stated

Statistical methods used: Not stated

Test method/conditions remarks: 24 hours of exposure. 

RESULTS

Endpoint value(s): LD50 > 2000 mg/kg-bw

Results remarks: Rabbits exhibited no symptoms.
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CONCLUSIONS

STUDY RELIABILITY

[4] Not assignable.

REFERENCE

Safety Data Sheet Bayer AG, 09.02.1999
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B-5. SKIN IRRITATION STUDY

Study 1: Skin irritation study in rabbits

Title: Primary Skin Irritation Study in Rabbits: Albemarle BQ451-8 Biocide: Lab Project 

Number: 7468: P326

TEST SUBSTANCE

Identity: 1H-Benzotriazole, 4(or 5)-methyl-, sodium salt, CAS No. 64665-57-2

Remarks: 50% aqueous solution of sodium tolyltriazole 

METHOD

Method/guideline followed: OECD Guide-line 404 "Acute Dermal Irritation/Corrosion"

Year: 1983

GLP (Y/N): No data

Species/strain: Rabbit

Sex (males/females/both): Not stated

Number of animals/sex/dose: 3/sex not specified

Volume administered: Not stated

Test conditions: 

Concentration: Undiluted

Exposure: Semiocclusive

Exposure Time: 4 hours

RESULTS

Result: Corrosive

CONCLUSIONS

RELIABILITY CODE

[1] Reliable without restriction.

Reliability remarks: Guideline study
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REFERENCES

Bayer AG data, Suberg H., Prüfung auf primär reizende/ätzende Wirkung an der Kaninchenhaut, 

January, 1984.
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CAS No. 64665-57-2: Summary of Endpoints

Endpoint Study Information 

Available

Acceptable Data Gap

Aquatic Effects

Acute Toxicity to Fish Yes Yes No

Acute Toxicity to Daphnia Yes Yes No

Toxicity to Aquatic Plants Yes Yes No

Health Effects

Acute Toxicity Yes Yes No

Repeated-Dose Toxicity No No Yes

Gene Mutation No No Yes

Chromosomal Aberrations No No Yes

Reproductive Toxicity No No Yes

Developmental Toxicity No No Yes