The FracPond
FracPit Components

Sulfates (SO4-2)
FracPit Components 

Sulfates (SO4-2)

Iron
FracPit Components 

Sulfates (SO4-2)

Iron

COD (Chemical Oxygen Demand)

 Measurement of Organics in Water

 Organic SRB/APB Foodsource

 Glycols and low molecular weight organic acids

 Functional moeities of organic matter

 Methanol, Minor Alcohols, Gasoline Range Organics, 

Diesel Range Organics, Guar Gel Residues
FracPit Components 

Desulfovibrio

Sulfates (SO4-2)

Iron

COD

SRB
FracPit Components 

Sulfates (SO4-2)

Iron

COD

SRB

H2S
FracPit Components 

Sulfates (SO4-2)

Iron

COD

SRB

H2S

Iron Sulfide
Iron Sulfide Production
FracPit Components 

SRB

COD

Sulfates (SO4-2)

H2S

Iron

Iron Sulfide
FracPit Treatment Methodologies

 Reduces COD (Organic Loadings) in frac pits

 Remediates Total Suspended Solids

 Non-Regulated

 Positive environmental impact

 Environmentally acceptable alternative to biocide 

treatments for SRB control in frac pits

 Sustained Positive Equilibrium Shift

 Cost-effective

Bioremediation
 Beneficial opportunistic extremophile rod bacterium

 Facultative Anaerobic

 Halophile (salt-loving)

 Nitrate-reducing (NRB)

 Non slime-producing

 Non spore-forming

Frac Pit Treatment Methodologies

Production Strain
Biological Control of SRB in FracPit Water

 Beneficial microbes introduce competition for SRB in frac 

pit environments. Beneficial microbes consume organic 

carbon sources (COD)

BSP1622 COD O2/NO3

CO2

+

Energy



FracPit Treatment Methodologies

 250,000 bbl recycle portadam facility in Garvin Co, OK

Bioremediation Case Study 

Southern OK Recycle Facility
FracPit Treatment Methodologies

 250,000 bbl recycle portadam facility in Garvin Co, OK

Bioremediation Case Study 

Southern OK Recycle Facility
FracPit Treatment Methodologies

 250,000 bbl recycle portadam facility in Garvin Co, OK

Bioremediation Case Study 

Southern OK Recycle Facility
50,000 Bbl Treatment Pit

250,000 Bbl Portadam

To Frac Job

BSP

FracPit Treatment Methodologies

 250,000 bbl recycle portadam facility in Garvin Co, OK

Bioremediation Case Study 

Southern OK Recycle Facility
Bioremediation Case Study 

Southern OK Recycle Facility

Treatment Pit

250,000 Bbl Portadam

To Frac Job

BSP

FracPit Treatment Methodologies

 250,000 bbl recycle portadam facility in Garvin Co, OK
FracPit Treatment Methodologies

 Aerated bioremediation recycling program from 

06/25/2013 thru 10/04/2013 (3 months).

COD

Bioremediation Case Study 

Southern OK Recycle Facility
FracPit Treatment Methodologies

COD

 COD’s decreased from avg 4,390 mg/L to 862 mg/L

Bioremediation Case Study 

Southern OK Recycle Facility
FracPit Treatment Methodologies

 Inlet SRB’s decreased from 6 bottles in 3 days to 2-4 vials 

in 10 days.

Bioremediation Case Study 

Southern OK Recycle Facility
FracPit Treatment Methodologies


 Millipore initial: 13 mL in 5 min w/0.45 μm filter @ 20 psi 

N

 Millipore final: 62 mL in 5 min w/0.45 μm filter @ 20 psi 

N
FracPit Treatment Methodologies
FracPit Treatment Methodologies
FracPit Treatment Methodologies

 3 months – 06/28/2013 thru 10/09/2013

 COD average decrease of 80.3%

 SRB’s in Portadam exponentially decreased from 

≥1,000,000 cells/mL to 3,000 cells/mL

 Total Suspended Solids/iron sulfide remediated

 Bioremediated water program deemed successful

Bioremediation Case Study Southern 

OK Recycle Facility
FracPit Treatment Methodologies
FracPit Treatment Methodologies
FracPit Treatment Methodologies
FracPit Treatment Methodologies

 Cost-effective

 Sustained equilibrium shift achieved by constant 

microbial activity in pit 

 COD depletion

 Managed SRB populations

 Removes iron from the system by forming iron carbonate 

and iron oxide scales which settle out in the pit.

 Non-regulated

 Can’t sterilize a frac pit

Bioremediation Benefits