### United States Office of Water EPA-822-R-07-001 Environmental Protection 4304T February 2007 Agency 

# AQUATIC LIFE AMBIENT FRESHWATER QUALITY CRITERIA - COPPER

2007 Revision

# AQUATIC LIFE AMBIENT FRESHWATER QUALITY CRITERIA - COPPER

2007 Revision

(CAS Registry Number 7440-50-8)

February 2007

U.S. Environmental Protection Agency

Office of Water Office of Science and Technology Washington, DC

# ii NOTICES

This document has been reviewed in accordance with U.S. EPA policy and approved for publication. Mention of trade names or commercial products does not constitute endorsement or

recommendation for use.

This document can be downloaded from EPA’s website at: http://www.epa.gov/waterscience/criteria/aqlife.html

# iii

# iv ACKNOWLEDGMENTS

Document Update: 2007

Luis A. Cruz (document coordinator and contributor)

U.S. EPA Health and Ecological Effects Criteria

Division Washington, DC

Cindy Roberts (contributor)

U.S. EPA Office of Research and Development Washington, DC

Mary Reiley (contributor)

U.S. EPA Health and Ecological Effects Criteria

Division Washington, DC

Robert Santore (contributor) HydroQual, Inc. Syracuse, New York

Paul Paquin (contributor) HydroQual, Inc. Syracuse, New York

Gary Chapman (contributor)

Great Lakes Environmental Center Columbus, Ohio Jennifer Mitchell (contributor) U.S. EPA (formerly) Health and Ecological Effects Criteria

Division Washington, DC

Charles Delos (contributor)

U.S. EPA Health and Ecological Effects Criteria

Division Washington, DC

Joseph Meyer (contributor) University of Wyoming Laramie, Wyoming

Rooni Mathew (contributor) HydroQual, Inc. Syracuse, New York

Tyler K. Linton (contributor)

Great Lakes Environmental Center Columbus, Ohio

Statistical Support and Contributor:

Russell Erickson Office of Research and Development Environmental Research Laboratory Duluth, Minnesota

# v CONTENTS

Notices

.................................................................... ii

Foreword

.................................................................. iii Acknowledgments

............................................................ iv

Contents

................................................................... v Acronyms

................................................................. vii

1.0 INTRODUCTION

....................................................... 1

2.0 APPROACHES FOR EVALUATING COPPER BIOAVAILABILITY

............ 2

2.1 General Aspects of Copper Bioavailability

.................................. 2 2.2 Existing Approaches

.................................................. 4

2.3 The BLM and Its Application to Criteria Development

........................ 5

2.4 BLM Uncertainties and Performance

...................................... 7

3.0 INCORPORATION OF BLM INTO CRITERIA DEVELOPMENT PROCEDURES 11

3.1 General Final Acute Value (FAV) Procedures

.............................. 11

3.2 BLM Input Parameters

............................................... 12

3.3 Data Acceptability and Screening Procedures

.............................. 12

3.4 Conversion Factors

.................................................. 14 3.5 Final Chronic Value (FCV) Procedures

................................... 14

4.0 DATA SUMMARY AND CRITERIA CALCULATION

........................ 14

4.1 Summary of Acute Toxicity to Freshwater Animals and Criteria Calculation

.... 14

4.1.1 Comparison with Earlier Hardness-Adjusted Criteria

..................... 16

4.2 Formulation of the CCC

........................................... 17

4.2.1 Evaluation of Chronic Toxicity Data

.................................. 17

4.2.2 Calculation of Freshwater CCC

..................................... 18

5.0 PLANT DATA

......................................................... 20

6.0 OTHER DATA

......................................................... 21

7.0 NATIONAL CRITERIA STATEMENT

.................................... 22

8.0 IMPLEMENTATION

................................................... 22

9.0 REFERENCES

........................................................ 43

# vi FIGURES

Figure 1. Conceptual Diagram of Copper Speciation and Copper-Gill Model

............... 5 Figure 2. Effects of Increasing Ion Concentration on Acute Lethality To Fathead Minnows

.... 9 Figure 3. Comparison of Predicted and Measured Acute Copper Toxicity to P. promelas

..... 10 Figure 4. Ranked Freshwater Genus Mean Acute Values (GMAVs)

..................... 15 Figure 5. Comparison of Hardness Based and BLM Based WQC (Alkalinity and pH Covary

with Hardness)

.................................................... 16 Figure 6. Relationship Between Freshwater Acute Copper Sensitivity (LC50 or EC50)

and Acute-Chronic Ratios

............................................. 19

TABLES

Table 1. Acute Toxicity of Copper to Freshwater Animals

........................... 24

Table 2a. Chronic Toxicity of Copper to Freshwater Animals

......................... 34

Table 2b. Chronic Toxicity of Copper to Saltwater Animals

........................... 36

Table 2c. Acute-Chronic Ratios

................................................ 37

Table 3a. Ranked Freshwater Genus Mean Acute Values

with Species Mean Acute-Chronic Ratios

................................. 38 Table 3b. Freshwater Final Acute Value (FAV) and Criteria Calculations

................. 39

Table 4. Toxicity of Copper to Freshwater Plants

.................................. 40

APPENDICES

Appendix A. Ranges in Calibration and Application Data Sets

........................ A-1 Appendix B. Other Data on Effects of Copper on Freshwater Organisms

............... B-1 Appendix C. Estimation of Water Chemistry Parameters for Acute Copper Toxicity Tests

. . C-1 Appendix D. Saltwater Conversion Factors for Dissolved Values

..................... D-1 Appendix E. BLM Input Data and Notes

....................................... E-1 Appendix F. Regression Plots

................................................ F-1 Appendix G. Example WQC Values Using the BLM and the Hardness Equation

......... G-1 Appendix H. Unused Data

................................................... H-1

# vii ACRONYMS

ACR Acute-Chronic Ratio

BL Biotic Ligand

BLM Biotic Ligand Model

CCC Criterion Continuous Concentration

CF Conversion Factors

CMC Criterion Maximum Concentration

CWA Clean Water Act

DIC Dissolved Inorganic Carbon

DOC Dissolved Organic Carbon

DOM Dissolved Organic Matter

EC Effect Concentration

EPA Environmental Protection Agency

FACR Final Acute-Chronic Ratio

FAV Final Acute Value

FCV Final Chronic Value

FIAM Free Ion Activity Model

GMAV Genus Mean Acute Value

GSIM Gill Surface Interaction Model

LC50 Lethal Concentration at 50 Percent Effect Level

LOAEC Lowest Observed Adverse Effect Concentration NASQAN National Stream Quality Accounting Network

NOAEC No Observed Adverse Effect Concentration pH Negative logarithm of the concentration (mol/L) of the H 3 

# O + 

# [H + 

# ] ion; scale range

from 0 to 14

SMAV Species Mean Acute Values

STORET EPA STOrage and RETrieval Data System

WER Water-Effect Ratio

WET Whole Effluent Toxicity WQC Water Quality Criteria

# 1

1.0 INTRODUCTION

Copper is an abundant trace element found in the earth's crust and is a naturally occurring element that is generally present in surface waters (Nriagu, 1979). Copper is a micronutrient for both plants and animals at low concentrations and is recognized as essential to virtually all plants and animals (Kapustka et al., 2004). However, it may become toxic to some forms of aquatic life at elevated concentrations. Thus, copper concentrations in natural environments, and its biological availability, are important. Naturally occurring concentrations of copper have been reported from

0.03 to 0.23 : 

# g/L in surface seawaters and from 0.20 to 30 : 

# g/L in freshwater systems (Bowen, 1985). Copper concentrations in locations receiving anthropogenic inputs can vary anywhere from levels that approach natural background to 100 : 

# g/L or more (e.g., Lopez and Lee, 1977; Nriagu, 1979; Hem, 1989) and have in some cases been reported in the 200,000 : 

# g/L range in mining areas (Davis and Ashenberg, 1989; Robins et al., 1997). Mining, leather and leather products, fabricated metal products, and electric equipment are a few of the industries with copper-bearing discharges that contribute to anthropogenic inputs of copper to surface waters (Patterson et al., 1998).

Over the past 20 years, the U.S. Environmental Protection Agency (EPA) has published a number of guidance documents containing aquatic life criteria recommendations for copper (e.g., U.S. EPA 1980, 1985, 1986, 1996). The present document contains EPA's latest criteria recommendations for protection of aquatic life in ambient freshwater from acute and chronic toxic effects from copper. These criteria are based on the latest available scientific information, supplementing EPA's previously published recommendations for copper. This criteria revision incorporated new data on the toxicity of copper and used the biotic ligand model (BLM), a metal bioavailability model, to update the freshwater criteria. With these scientific and technical revisions, the criteria will provide improved guidance on the concentrations of copper that will be protective of aquatic life. The BLM is not used in the saltwater criteria derivation because further development is required before it will be suitable for use to evaluate saltwater data.

This document provides updated guidance to states and authorized tribes to establish water quality standards under the Clean Water Act (CWA) to protect aquatic life from elevated copper exposure. Under the CWA, states and authorized tribes are to establish water quality criteria to protect designated uses. Although this document constitutes EPA's scientific recommendations regarding ambient concentrations of copper, it does not substitute for the CWA or EPA's regulations, nor is it a regulation itself. Thus, it cannot impose legally binding requirements on EPA, states, tribes, or the regulated community, and might not apply to a particular situation based on the circumstances. State and tribal decision makers retain the discretion in adopting approaches, on a case-by-case basis, that differ from this guidance when appropriate. EPA may change this guidance

in the future.

Although the BLM has been used in place of the formerly applied hardness-based approach, the updated freshwater criteria derivations in this document are still based on the principles set forth

in the Guidelines for Deriving Numerical Water Quality Criteria for the Protection of Aquatic Life

and Their Uses

(Stephan et al. 1985, hereafter referred to as the Guidelines). Section 2 of this document provides an overview of copper bioavailability and the BLM. Additional information on the generalized BLM framework, theoretical background, model calibration, and application for the BLM can be found in the published literature. Section 3 of this document discusses general

# 2

procedures and requirements for applying the BLM to criteria. Section 4 provides the derivation of criteria Final Acute Value (FAV) and Final Chronic Value (FCV) for freshwater organisms. Section 5 discusses plant data and Section 6 discusses other data not included in the criteria derivation. Sections 7 and 8 provide the final criteria statements and information on implementation. Various supplementary information is provided in several appendices.

2.0 APPROACHES FOR EVALUATING COPPER BIOAVAILABILITY

2.1 General Aspects of Copper Bioavailability

The toxicity of a chemical to an aquatic organism requires the transfer of the chemical from the external environment to biochemical receptors on or in the organism at which the toxic effects are elicited. Often, this transfer is not simply proportional to the total chemical concentration in the environment, but varies according to attributes of the organism, chemical, and exposure environment so that the chemical is more or less "bioavailable". Definitions of bioavailability vary markedly (e.g., National Research Council, 2003) and are often specific to certain situations, but a useful generic definition is the relative facility with which a chemical is transferred from the environment to a specified location in an organism of interest.

Of particular importance to bioavailability is that many chemicals exist in a variety of forms (chemical species). Such chemical speciation affects bioavailability because relative uptake rates can differ among chemical species and the relative concentrations of chemical species can differ among exposure conditions. At equilibrium in oxygenated waters, "free" copper exists as cupric ion - Cu(II) weakly associated with water molecules (Cu

. 

# nH 2 

# O +2 

# ), but this species is usually a small percentage of the total copper. Most dissolved copper is part of stronger complexes with various ligands (complexing chemicals that interact with metals), including dissolved organic compounds, hydroxides, carbonates, and other inorganic ligands. Substantial amounts of copper can also be adsorbed to or incorporated into suspended particles. More information on copper speciation in freshwater can be found in Kramer et al. (1997), Bryan et al. (2002), and Smith et al. (2002).

Copper toxicity has been reported to vary markedly due to various physicochemical characteristics of the exposure water (e.g., either laboratory or field), including temperature, dissolved organic compounds, suspended particles, pH, and various inorganic cations and anions, including those composing hardness and alkalinity (see reviews by Sprague, 1968; Hunt, 1987; Campbell, 1995; Allen and Hansen, 1996; Paquin et al., 2002). Many of these physicochemical factors affect copper speciation, and their effects on copper toxicity therefore could be due to effects on copper bioavailability. That bioavailability is an important factor is evident from uptake of copper by aquatic organisms being reduced by various organic compounds and inorganic ligands known to complex copper (Muramoto, 1980; Buckley et al., 1984; Playle et al., 1993 a,b; MacRae et al., 1999).

A "ligand" is a complexing chemical (ion, molecule, or molecular group) that interacts with a metal like copper to form a larger complex. A “biotic ligand” is a complexing chemical that is a component of an organism (e.g. chemical site on a fish gill). For certain ligands, some studies have demonstrated that the concentration of free copper associated with a specified level of accumulation or toxicity changes little as the ligand concentration is varied, despite major changes in the

# 3

proportion of copper bound to the ligand (see review by Campbell, 1995). This suggests that, even at low concentrations, free copper is more important to bioavailability than the ligand-bound copper. This is expected if accumulation and toxicity are dependent on the binding of copper to a biochemical receptor "X" on the surface of the organism, forming a chemical species X-Cu (receptor-bound metal) that is a first limiting step in accumulation and toxicity. By standard chemical equilibrium expressions, the amount of such species and the consequent biological effects would be a function of the activity of just free copper (Morel, 1983 a), a relationship commonly referred to as the free ion activity model (FIAM). Ligand-bound copper (Cu-L) would contribute to copper bioavailability if (a) a species X-Cu-L is formed that is important to copper accumulation/toxicity, (b) the microenvironment near the organism surface is such that Cu-L dissociates and increases the free copper activity interacting with "X", or (c) copper uptake is via mechanisms that do not entail binding to such a receptor and can accommodate different copper species. Some studies have indicated dissolved complexes of copper do contribute to bioavailability (reviews by Sprague, 1968; Hunt, 1987; Campbell, 1995; Allen and Hansen, 1996; Paquin et al., 2002).

The effects of physicochemical factors on copper toxicity are diverse and the specific chemistry of the exposure water will determine whether or not there are appreciable effects on copper speciation and a resulting strong relationship of toxicity to free copper. Usually copper toxicity is reduced by increased water hardness (reviews by Sprague, 1968; Hunt, 1987; Campbell, 1995; Allen and Hansen, 1996; Paquin et al., 2002), which is composed of cations (primarily calcium and magnesium) that do not directly interact with copper in solution so as to reduce bioavailability. In some cases, the apparent effect of hardness on toxicity might be partly due to complexation of copper by higher concentrations of hydroxide and/or carbonate (increased pH and alkalinity) commonly associated with higher hardness. However, significant effects on toxicity often are still present when hardness is increased in association with anions which do not interact strongly with copper (Inglis and Davis, 1972; Chakoumakos et al., 1979; Miller and Mackay, 1980; Erickson et al., 1987). Hardness cations could have some limited effect on copper speciation by competing with copper for the same dissolved ligands, but increased hardness would then increase free copper and thus increase, not decrease, toxicity. Sodium has also been reported to affect copper toxicity (Erickson et al., 1996 b) and pH effects can be partly due to effects of hydrogen ion other than on copper speciation (Peterson et al., 1984).

The effects of hardness cations could be explained by the competing with copper for the biochemical receptor "X", thus reducing copper uptake (Zitko, 1976; Zitko et al., 1976; Pagenkopf, 1983). Reduced metal bioavailability due to increased hardness cations has been experimentally demonstrated (Playle et al., 1992; Meyer et al., 1999, 2002), although this does not specifically establish cation competition as the mechanism. Pagenkopf (1983) provided a mathematical description of a Gill Surface Interaction Model (GSIM) that addressed the effects on metal toxicity of both metal speciation and cations via the interactions of gill surface biochemical receptors with the free toxic metal, other metal species, hardness cations, and hydrogen ion.

The empirical evidence demonstrates that copper toxicity is affected by exposure conditions and that much of these effects is plausibly attributed to effects of ligands and cations on copper bioavailability. However, it should not be presumed that all of the observed effects of the physicochemical factors on copper toxicity reflect effects on bioavailability, or that bioavailability

# 4

effects are just due to ligand complexation and cation competition. For example, acute copper toxicity in aquatic organisms has been related to disruption of osmoregulation, specifically sodium/potassium exchange (Lauren and MacDonald, 1986; Wood, 1992; Wood et al., 1997; Paquin et al., 2002), which can be affected by calcium other than by competition with copper for the same biochemical receptor. Similarly, reported effects of sodium and potassium on copper toxicity (Erickson et al., 1996 b) might simply reflect favorable or unfavorable ion exchange gradients, rather than any effect on copper bioavailability. Nevertheless, the effects of ligand complexation and cation competition on copper bioavailability provide a reasonable conceptual framework for improved descriptions of how copper toxicity differs across exposure conditions.

2.2 Existing Approaches

EPA aquatic life criteria for metals address the reported effects of hardness on metal toxicity using empirical regressions of toxic concentrations versus hardness for available toxicity data across a wide range of hardness (Stephan et al., 1985). Such regressions provided the relative amount by which the criteria change with hardness, but have certain limitations. The regressions were not just of hardness, but of any other factor that was correlated with hardness in the toxicity data set used for the regressions, particularly pH and alkalinity. Although these regressions therefore address more bioavailability issues than hardness alone, they best apply to waters in which the correlations among hardness, pH, and alkalinity are similar to the data used in the regressions. The separate effects of these factors are not addressed for exposure conditions in which these correlations are different. In addition, some physicochemical factors affecting metal toxicity, such as organic carbon, are not addressed at all.

Existing EPA metals criteria also address bioavailability by using dissolved metal as a better approximation for metal bioavailability than total metal (U.S. EPA, 1993). Although this approach accounts for the low bioavailability of metal on suspended particles, it does not address the major effects of various dissolved species on bioavailability. This approach could conceivably be further developed to include just part of the dissolved copper, but this not only requires resolving what species to include, how to weight them, and how to assess their concentrations, but also would not address the effects of cations and other factors that affect toxicity in addition to metal speciation. Such a "bioavailable fraction" approach is not justified, because no fraction of metals species provides a constant measure of toxicity.

To address more completely the modifying effects of water quality than the hardness regressions achieve, EPA issued guidance in the early 1980s on the water-effect ratio (WER) method (Carlson et al., 1984; U.S. EPA, 1983, 1992, 1994). The WER is "a biological method to compare bioavailability and toxicity in receiving waters versus laboratory test waters" (U.S. EPA, 1992). A WER is calculated by dividing the acute LC50 of the metal, determined in water collected from the receiving water of interest, by the LC50 of the metal determined in a standard laboratory water, after adjusting both test waters to the same hardness. The standard laboratory water LC50 is used as the denominator to reflect that this LC50 is measured in test water that has water quality characteristics representative of the test waters used to develop the Water Quality Criteria (WQC) toxicity database, at least as a good approximation. The national hardness-based acute criterion concentration is then multiplied by this ratio (i.e., the WER) to establish a site-specific criterion that reflects the effect of site water characteristics on toxicity. However, a WER accounts only for

# 5

interactions of water quality parameters and their effects on metal toxicity to the species tested and in the water sample collected at a specific location and at a specific time. There is also significant cost to generate a single WER.

Because of the limitations of these past approaches for addressing bioavailability in metals criteria, there is a need for an approach that (1) explicitly and quantitatively accounts for the effect of individual water quality parameters that modify metal toxicity and (2) can be applied more cost-effectively and easily, and hence more frequently across spatial and temporal scales. An assessment framework that incorporates the bioavailability mechanisms discussed in Section 2.1 was therefore used to address more comprehensively the effects of physicochemical exposure conditions on copper toxicity with lower costs than required by the WER approach.

2.3 The Biotic Ligand Model and Its Application to Criteria Development

The interactions of toxic metal species and other exposure water constituents with biological surface receptors described by Zitko (1976), Morel (1983), and Pagenkopf (1983) provided the basic conceptual and mathematical structure for the bioavailability model to be used here (Figure 1). Subsequent experimental work has supported various model tenets by demonstrating the effects of complexing ligands and competing cations on accumulation of toxic metals at fish gills and the relationship of toxic effects to accumulation, and has also provided estimates of various model parameters (Playle et al., 1992, 1993a,b; Janes and Playle, 1995; MacRae et al., 1999, Meyer et al., 1999, 2002; McGeer et al., 2002). Various efforts in metal speciation modeling also have provided the ability to do better speciation calculations, especially regarding complexation of metals by organic matter (e.g., Tipping, 1994). This experimental work has supported further metal toxicity model development (Meyer, 1999; Brown and Markich, 2000; McGeer et al., 2002; Di Toro et al., 2001; Santore et al., 2001; Paquin et al., 2002). This bioavailability modeling approach is now commonly termed “Biotic Ligand Models” to broaden the scope beyond gill surfaces and to acknowledge that the biochemical receptor "X" discussed in Section 2.1 is a metal-binding ligand that is treated similarly to ligands in the exposure water, except that it is on the organism and is the keystone for metal accumulation and toxicity.

# 6 Equation 1

Equation 2

Equation 3

Equation 4 Briefly, available evidence indicates that both free copper and copper monohydroxide bind to a biotic ligand "Lb" on the organism's surface (Lb-Cu and Lb-CuOH) and that death occurs when a certain amount of the total biotic ligand sites are occupied by copper. This ligand must be at the organism surface because the model describes its interactions with the external exposure water. However, this does not mean that this ligand is the site of toxic action; rather it is only necessary to assume that copper accumulation at the site(s) of toxic action is proportional to binding at the biotic ligand (i.e., the biotic ligand controls bioavailability). Other cations also will bind to the biotic ligand, affecting copper bioavailability because higher concentrations of copper are needed for copper to reach toxic levels. The binding to the biotic ligand is considered to be at equilibrium, with apparent (activity-corrected) equilibrium constants K LbCu 

# , K

LbCuOH 

# , and K

LbCj 

# , respectively, for free copper, copper hydroxide, and the "jth" competing cation. Chemical speciation in the exposure water is also considered to be at equilibrium, and chemical speciation calculations are conducted to compute the free copper, copper hydroxide, and competing cation activities to which the biotic ligand is exposed. Because binding to the actual biotic ligand cannot be measured, it is expected that accumulation relationships for some measurable variable (e.g., the total metal in gill tissue) provide a reasonable surrogate for the actual biotic ligand. Because criteria deal with concentrations eliciting a certain level of effects on groups of organisms (e.g., LC50s), model calculations are for an organism with characteristics appropriate for such group-wide statistics.

How the BLM is applied to criteria can be best discussed by starting with the following general expression for the BLM:

where EC is the total dissolved copper concentration eliciting an effect, EC 0 

# is a baseline EC in the absence of any complexing ligands and competing cations, f C 

# should be a factor (<1) for how much competing cations increase EC, and f L 

# should be a factor (<1) for how much complexing ligands

increase EC. For the BLM used here:

where f

LbT 

# is the fraction of the biotic ligand sites that must be occupied by copper to elicit the toxicity of interest (e.g., a lethal accumulation divided by the accumulation capacity), m is the

# 7 Equation 5 number of competing cations included in the model, [Cj] is the concentration of the jth competing cation, " Cu+2 

# is the ratio of free copper concentration to total dissolved copper concentration, " CuOH 

# is the ratio for the copper hydroxide complex, and the ratio K LbCuOH 

# /K LbCu 

# specifies the bioavailability of CuOH relative to free copper. Thus, in the absence of complexing ligands and competing cations, the toxic concentration is only a function of the binding strength of free copper and the copper occupied fraction of biotic ligand sites needed to elicit toxicity. The increase in the effect concentration due to competing cations is simply a sum of the products of their concentrations and binding constants. The increase in the effect concentration due to complexing ligands is the inverse of the sum of the products of the relative bioavailabilities and concentration fractions of the species that bind to the biotic ligand (free copper and copper hydroxide).

If toxicity to all the biological species in the criteria (at least the most sensitive ones) were determined based on measured accumulation properties and the relationship of toxicity to accumulation, the above model equations would be directly applied in criteria calculations. However, this is not the case. Although gill accumulation properties and lethal accumulations have been measured for certain species and conditions, and this has been useful in validating BLM assumptions and formulations, the data that must be applied to the criteria consists of water effect concentration (ECs) for biological species for which this accumulation information is generally not available. The BLM therefore is needed, not to make absolute calculations regarding toxic concentrations, but to extrapolate toxic concentrations from one exposure condition to another:

where the A and B subscripts refer to different exposure conditions. The general procedure that was followed for criteria development here was to use the above equation to normalize all available toxicity data to a reference exposure condition, calculate criteria values at the reference condition, and again use the above equation to compute criteria at other conditions.

This means that the BLM assumptions and parameters that just pertain to EC 0 

# are not important to its application to criteria, which actually simplifies model validation and parameterization needs. In particular, there is no need to estimate f LbT 

# , or the lethal accumulations and accumulation capacities that define this fraction. Furthermore, the absolute values of K LbCu 

# and

K LbCuOH 

# do not need to be known, only their relative value (and if copper binding to the biotic ligand was dependent only on free copper, the value of K LbCu 

# would not be needed at all). Absolute values are only needed for the binding constants for the competing cations, as well as the various constants needed in speciation calculations to estimate " Cu+2 

# and " CuOH 

# . For BLM application to criteria, the important concern is whether f C 

# and f

L 

# are suitably formulated and parameterized, and not with issues that relate to lethal accumulations and accumulation capacities.

2.4 BLM Uncertainties and Performance

The BLM employed here uses equilibrium reactions of copper and other cations with a single, simple type of surface ligand as the focus for all the effects of physicochemical exposure conditions on toxicity, and thus is a simple, approximate representation for the complex set of chemical

# 8

reactions and transfers involved with environmental copper concentrations eliciting toxicity. As already noted, cation effects might involve mechanisms other than competition for a surface ligand. The microenvironment at the gill might change copper speciation. Multiple mechanisms that do not react the same to external conditions might be involved in copper bioavailability and toxicity. Accumulation parameters based on bulk gill measurements will likely not be the same as those for the biotic ligand. Nonequilibrium processes might be important, especially regarding the relationship of copper-binding on a surface ligand to toxic action.

However, any model is a simplification of reality and the existence of uncertainties does not preclude a model from being useful and justified. Despite its simplicity, the BLM used here provides a reasonable mechanistic framework for the well-established effects of copper speciation, explicitly addressing the relative bioavailability of different copper species. It also includes a plausible mechanism that allows the effects of cations to be addressed and uses a comprehensive model for calculating the required concentrations of various chemical species. Even if the mechanistic descriptions are incomplete, this model allows the major empirical effects of complexing ligands and competing cations to be described in a more comprehensive and reasonable fashion than other approaches.

Because this model is used in criteria to predict relative effects of physicochemical exposure factors, its utility for criteria can be judged based on how well it predicts the relative effects of these factors in copper toxicity studies. Examples of BLM performance for various exposure factors and studies are provided in the technical support document for this criteria. Figure 2 shows one example from a study on the effects of various exposure conditions on the acute lethality of copper to fathead minnows. This set of exposures consisted of synthetic exposure solutions of various total ion concentrations with fixed ratios of the major cations and anions, at a fixed pH (8.0) and low dissolved organic matter (< 0.5 mg/L). Observed dissolved LC50s (solid circles with uncertainty bars) varied by 24-fold for only a 9-fold change in total ions. These large effects reflect the combined influences of increased alkalinity (copper carbonate complex formation), hardness, and sodium. Considering the wide range of the observed LC50s and that the model was not fitted to these data, BLM-predicted LC50s (open symbols) were rather accurate, ranging from 55 to 87% (average 75%) of the observed value. More importantly for criteria, the predicted relative change across the range of total ion concentration was 20-fold, very close to that observed.

# 9

Model performance can also be judged across a variety of factors as in Figure 3, which shows predicted versus observed LC50s for a large number of exposures in the cited study, which varied hardness, alkalinity, sodium, and pH together and separately over a wide range. Observed LC50s varied by about 60-fold, but predicted values deviated from observed values by only 0.12 log units (a factor of 1.3) on average, and at worst only slightly more than a factor of 2. Again, more information on model performance is provided in the Technical Support Document and the figures here just provide some examples demonstrating the utility of this model for use in criteria.

# 10

The use of the BLM to predict the bioavailability and toxicity of copper to aquatic organisms under site-specific conditions is a significant change from the previous Criterion Maximum Concentration (CMC) derivation methodology. Previous aquatic life criteria documents for copper (e.g., U.S. EPA, 1980, 1985, 1996) expressed the CMC as a function of water hardness. Now, EPA chooses to utilize the BLM to update its freshwater acute criterion because the BLM accounts for all important inorganic and organic ligand interactions of copper while also considering competitive interactions that influence binding of copper at the site of toxicity, or the "biotic ligand." The BLM's ability to incorporate metal speciation reactions and organism interactions allows prediction of metal effect levels to a variety of organisms over a wide range of water quality conditions. Accordingly, the BLM is an attractive tool for deriving water quality criteria. Application of the BLM has the potential to substantially reduce the need for site-specific modifications, such as Water Effect Ratio, to account for site-specific chemistry influences on metal toxicity.

The updated BLM-based WQC will in some cases be more stringent and in other cases less stringent than the hardness based WQC. As there is not a single WQC value to use for comparison purposes, it will only be possible to provide illustrative examples of each situation. It is the judgement of the EPA that the BLM-based WQC for Cu will provide an improved framework for evaluating a level of protection (LOP) that is consistent with the LOP that was intended by the 1985 Guidelines (i.e., a 1-in-3 year exceedance frequency that will be protective of 95% of the genera).

While the BLM is currently considered appropriate for use to derive an updated freshwater CMC for the acute WQC, further development is required before it will be suitable for use to +2

-2

# 11 Equation 6

Equation 7 evaluate a saltwater CMC or a Criterion Continuous Concentration (CCC) or chronic value (freshwater or saltwater WQC).

3.0 INCORPORATION OF THE BLM INTO CRITERIA DERIVATIONS

PROCEDURES

3.1 General Final Acute Value (FAV) Procedures

Application of the acute copper BLM to the derivation of the copper FAV is analogous to procedures already described in the Guidelines for metals criteria using empirical hardness regressions. For these hardness-dependent metals criteria, LC50s at various hardness are normalized to a reference hardness using the regression slopes. The normalized LC50s for each biological species are averaged to derive Species Mean Acute Values (SMAVs) at the reference hardness. The SMAVs within each genus are then averaged to derive Genus Mean Acute Values (GMAVs) at the reference hardness. The Guidelines’ procedures for estimating the fifth percentile

of the GMAVs are then used to derive the FAV at the reference hardness. FAVs for other hardness can then be derived using the hardness regression slope, and these FAVs are used to calculate the Criterion Maximum Concentration (CMC) by dividing the FAV by 2.0 and the Final Chronic Values (FCV) by dividing the FAV by the Final Acute-Chronic Ratio (FACR). Following the Guidelines, the Criterion Continuous Concentration (CCC) is set to the FCV unless other data justifies a lower

value.

Extending this procedure to apply the BLM simply involves normalizing the LC50s to a reference exposure condition that includes all the physicochemical exposure factors important to the BLM, not just hardness. For this normalization, the BLM provides the factors f C 

# and f

L 

# discussed in Section 2.3, these factors serving the same purpose as the hardness regression slope described above. Each LC50 to be used in criteria derivation would be normalized to the reference exposure conditions by the equation:

where the subscript A refers to the exposure conditions for the observed LC50 and the subscript R refers to the reference exposure conditions to which the LC50 is being normalized. These normalized LC50s are then used to derive the SMAVs, GMAVs, and FAV at the reference exposure condition as described above for the hardness-corrected criteria. The BLM is then used to derive FAVs at other exposures by the equation:

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where the subscript B refers to the exposure conditions for which an FAV is desired. These BLM-derived FAVs are then used to derive CMCs and CCCs following standard Guidelines procedures.

For the criteria in this document, the reference exposure conditions to which LC50s are normalized and at which the reference FAV is calculated are as follows (see also footnote f in Table 1). The water chemistry used in the normalization was based on the EPA formulation for moderately-hard reconstituted water, but any other water chemistry could have been used. In this formulation the parameters included: temperature = 20 o 

# C, pH = 7.5, DOC = 0.5 mg/L, Ca = 14.0 mg/L, Mg = 12.1 mg/L, Na = 26.3 mg/L, K = 2.1 mg/L, SO 4 

# =81.4 mg/L, Cl = 1.90 mg/L, Alkalinity = 65.0 mg/L and S = 0.0003 mg/L.

3.2 BLM Input Parameters

For applying an LC50 to criteria derivations and for determining an FAV at exposure conditions of interest, the necessary water quality input parameters for BLM calculations are temperature, pH, dissolved organic carbon, major geochemical cations (calcium, magnesium, sodium, and potassium), dissolved inorganic carbon (DIC, the sum of dissolved carbon dioxide, carbonic acid, bicarbonate, and carbonate), and other major geochemical anions (chloride, sulfate). DIC measurements are typically not made in the environment, and an alternative input parameter is alkalinity, which can be used with pH and temperature to estimate DIC. There is some evidence that other metals such as iron and aluminum can have an effect on copper toxicity to aquatic organisms, which might be due to interactions of these metals with the biotic ligand, effects of these metals on organic carbon complexation of copper, or adsorption of copper to iron and aluminum colloids which are present in filtrates used to measure dissolved copper. These metals are not currently included in routine BLM inputs, but users are encouraged to measure dissolved iron and aluminum as part of monitoring efforts to support possible future criteria applications.

A number of fixed parameters are also used in the BLM but are not required user inputs in criteria derivations. These include the variety of equilibrium constants used in copper speciation calculations, and also the binding constants for copper and various cations to the biotic ligand. The values for these constants were obtained from work by Playle and coworkers (Playle et al., 1992, 1993a,b) and also by inference from the relationship of toxicity to various water quality characteristics. More information about these parameters can be obtained from the technical support document.

3.3 Data Screening Procedures

To use a toxicity test in the derivation of BLM-based criteria, information must be available for the various water quality parameters described in Section 3.2. This is in contrast to past metals criteria, for which the only necessary water quality parameter was hardness. Many of these parameters are not routinely measured in toxicity tests and, if measured, are not necessarily reported in the primary literature for the test, especially for older toxicity tests. However, this information might be available from supplemental sources or be estimated based on other information. Therefore, in addition to reviewing the primary sources for relevant information,

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additional efforts were made to obtain or estimate the necessary water quality parameters for as many of the available LC50s as possible.

A detailed description of these efforts is provided in Appendix C, Estimation of Water Chemistry Parameters for Acute Copper Toxicity Tests, and are summarized as follows. Reports of acute copper toxicity tests identified in literature searches were reviewed to identify LC50s for possible inclusion in the criteria derivation. In addition to test acceptability standards specified in the Guidelines, the current effort also required that the LC50s be based on measured copper concentrations. LC50s based on nominal concentrations have been used in previous criteria, but there are enough measured LC50s for copper that this was considered to be no longer warranted, especially considering the more advanced bioavailability assessments represented by the BLM. For the identified LC50s, the primary reports were reviewed to record all reported information on dilution and test water chemistry. Any additional references specified by the authors were also obtained and reviewed. If test waters were synthetically prepared based on specified formulas, these were used to estimate parameters as appropriate. When critical water chemistry parameters were not available, authors were contacted regarding unpublished information or to measure missing water chemistry parameters in dilution source waters. If primary or corresponding authors could not be contacted, an attempt was made to contact secondary authors or personnel from the laboratories where the studies were conducted. Where actual water chemistry data were unavailable, data from other studies with the same water source were used as surrogate values if appropriate. Absent this, the U.S. Geological Survey's National Stream Quality Accounting Network (NASQAN) and the EPA STOrage and RETrieval (STORET) were used to obtain data

for ambient surface waters which were the source of water for a test. In some instances other available sources were contacted to obtained water chemistry data (e.g., city drinking water treatment personnel). The acquired data were scrutinized for representativeness and usefulness for estimating surrogate values to complete the water quality information for the dilution and/or test water that was used in the original studies. When the above sources could not be used, geochemical ion inputs were based on reported hardness measurements and regressions relationships constructed for the relationship of various ions to hardness from NASQAN data.

As with any modeling effort, the reliability of model output depends on the reliability of model inputs. Although the input data have been closely scrutinized, the reliability of the BLM-normalized LC50s are subject to the uncertainties of the estimation procedures described above. Therefore, a ranking system was devised to rank the quality of the chemical characterization of the test water. Studies with a rank of 1 contain all of the necessary parameters for BLM input based on measurements from either the test chambers or the water source. In general, studies in which the BLM input parameters were reported for test chamber samples take precedence over studies in which the parameters were reported only for the source water. A characterization ranking of 2 denotes those studies where not all parameters were measured, but reliable estimates of the requisite concentrations could be made. Similarly, a rank of 3 denotes studies in which all parameters except DOC were measured, but reliable estimates of DOC could be made. For the majority of the tests, a chemical characterization of 4+ was assigned because hardness, alkalinity, and pH were measured, and the ionic composition could be reliably estimated or calculated. A 4- was assigned to those studies conducted using standard reconstituted water in which hardness, alkalinity, or pH was either measured or referenced, and the recipe for the water is known (ASTM, 2000; U.S. EPA, 1993). The chemical characterization rank of 5 was ascribed to studies in which

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one of the key parameters (DOC, Ca, pH, alkalinity) was not measured, and when it could not be reliably estimated. If two or more key parameters (DOC, Ca, pH, alkalinity) were not measured and could not be reliably estimated, a study was given a chemical characterization rank of 6. Studies receiving a quality rating of greater than 4+ (i.e., higher than 4) were not used in the criteria development procedures because the estimates for some of the key input parameters were not thought to be reliable, all other studies were used.

3.4 Conversion Factors

The LC50s used in deriving previous EPA metals criteria were based on total metal concentration (measured or nominal) and the criteria were consequently for total metals

concentration. EPA afterwards made the decision that metals criteria should be based on dissolved metal because it was thought to better represent the bioavailable fraction of the metal (U.S. EPA, 1993). It was thus necessary to convert the criteria to a dissolved concentration basis. However, at that time, most toxicity tests reported only total concentration, so that a procedure was necessary to estimate the likely fractions of metals that were dissolved in typical toxicity tests. Studies were therefore conducted to determine these fractions under a variety of test conditions that mimicked the conditions in the tests used to derive the metals criteria (University of Wisconsin-Superior, 1995). These tests demonstrated high fractions of dissolved copper and resulted in a conversion factor (CF) of 0.96 for converting both the CMC and CCC for copper from a total to dissolved basis (Stephan, 1995). The BLM-derived criteria developed here also uses dissolved copper as the basis for criteria, assuming a negligible bioavailability for particulate copper. The conversion factor of 0.96 was also used to convert total to dissolved copper for any toxicity test for which dissolved copper measurements were not available.

3.5 Final Chronic Value (FCV) Procedures

Because the minimum eight family data requirements for chronic toxicity data were not met in order to calculate the FCV by the fifth percentile method used for the FAV and because insufficient information was available to develop a chronic BLM, EPA derived the CCC utilizing the Acute to Chronic Ratio (ACR) approach from the Guidelines (Stephan et al., 1985). To calculate the FCV at a specific water chemistry, the FAV at that chemistry is divided by the FACR. This entails the assumption that the acute BLM reasonably approximates the bioavailability relationships for chronic toxicity. Limited data available regarding effects of water chemistry on sublethal effects and chronic lethality do show substantial effects of organic matter, alkalinity, pH, and sodium (Winner, 1985; Erickson et al., 1996 a,b) similar to those in the acute BLM used here. For hardness, apparent effects are limited and uncertain, but the use of the acute BLM does not introduce major uncertainties in this regard because the effects of hardness by itself in the acute BLM are also

limited.

4.0 DATA SUMMARY AND CRITERIA CALCULATION

4.1 Summary of Acute Toxicity to Freshwater Animals and Criteria Calculation

The screening procedure outlined in Sec. 3.3 (high quality data = 1, low quality data > 4, e.g. 4+) identified approximately 600 acute freshwater toxicity tests with aquatic organisms and copper

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potentially acceptable for deriving criteria. Of these tests, approximately 100 were eliminated from the criteria derivation process because they did not report measured copper concentrations. Nearly 150 additional tests were eliminated from the calculation of the FAV because they received a quality rating of greater than 4 in the quality rating scheme described in section 3.3 described above.

Data from approximately 350 tests were used to derive normalized LC50 values, including 15 species of invertebrates, 22 species of fish, and 1 amphibian species (Table 1), representing 27 different genera. Species Mean Acute Values (SMAVs) at the reference chemistry were calculated from the normalized LC50s and Genus Mean Acute Values (GMAVs) at the normalization chemistry were calculated from the SMAVs.

SMAVs ranged from 2.37 μg/L for the most sensitive species, Daphnia pulicaria , to 107,860 μg/L for the least sensitive species, Notemigonus crysoleucas . Cladocerans were among the most sensitive species, with D. pulicaria, D. magna

, Ceriodaphnia dubia , and Scapholeberis sp. being four out of the six most sensitive species. Invertebrates in general were more sensitive than fish, representing the 10 lowest SMAVs.

The 27 GMAVs calculated from the above-mentioned SMAVs ranged from 4.05 μg/L for Daphnia to 107,860 μg/L for Notemigonus (Table 3a). Nine of the 10 most sensitive genera were invertebrates. The salmonid genus Oncorhynchus was the most sensitive fish genus, with a GMAV

of 31

. 39 μg/L and an overall GMAV ranking of 10.

The ranked GMAVs are presented in Figure 4. Pursuant to procedures used to calculate the FAV, a FAV of 4.67 : 

# g/L was derived from the four GMAVs with cumulative probabilities closest to the 5th percentile toxicity value for all the tested genera (Table 3b). The presumption is that this

Figure 4. Ranked Freshwater Genus Mean Acute Values (GMAVs)

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acute toxicity value represents the LC50 for an organism that is sensitive at the 5th percentile of the GMAV distribution. The CMC is the FAV divided by two. Therefore, the freshwater dissolved copper CMC for the reference chemistry presented is 2.337 μg/L.

Site-water chemistry parameters are needed to evaluate a criterion. This is analogous to the situation that previously existed for the hardness-based WQC, where a hardness concentration was necessary in order to derive a criterion. Examples of CMC calculations at various water chemistry conditions are presented in Figure 5 and Appendix G.

4.1.1 Comparison With Earlier Hardness-Adjusted Criteria

EPA’s earlier freshwater copper criteria recommendations were hardness-dependent values. One would expect a BLM-based criterion calculation procedure to yield the more appropriate criterion—appropriate in the sense that it accounts for the important water chemistry factors that affect toxicity, including DOC complexation, where the hardness correction does not. Application of the BLM in field situations where DOC is expected to be present at higher concentrations than those observed in laboratory studies would likely improve the performance of the BLM compared with the hardness adjustment. The reason is that the BLM would reasonably account for the typically observed increase in effect levels under such conditions, while the hardness-based approach would not (Figure 5).

As a comparison between the hardness typical of the previous copper criterion and this revised criterion using the BLM, both procedures were used to calculate criterion values for waters with a range in hardness as specified by the standard EPA recipes (U.S. EPA, 1993). The EPA formulations specify the concentration of various salts and reagents to be used in the synthesis of

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laboratory test waters with specific hardness values (e.g., very soft, soft, moderately hard, hard, or very hard). As the water hardness increases in these recipes, pH and alkalinity also increase. This has implications for the BLM because the bioavailability of copper would be expected to decrease with increasing pH and alkalinity due to the increasing degree of complexation of copper with hydroxides and carbonates and decreasing proton competition with the metal at both DOM and biotic ligand binding sites. The BLM criterion for these waters agrees very well with that calculated by the hardness equation used in previous copper criterion documents (Figure 5). However, alkalinity and pH change as hardness changes in the EPA recipes. The BLM prediction is taking all of these changes in water quality into account.

It is possible to use the BLM to look only at the change in predicted WQC with changes in hardness (e.g., alkalinity and pH remaining constant). The hardness equation is based on waters where changes in hardness are accompanied by changes in pH and alkalinity. However, there are many possible natural waters where changes in hardness are not accompanied by changes in pH and alkalinity (such as water draining a region rich in gypsum). In these cases, the hardness equation based criterion will still assume a response that is characteristic of waters where hardness, alkalinity, and pH co-vary, and will likely be underprotective relative to the level of protection intended by the Guidelines, in high hardness waters. Conversely, in waters where the covariation between hardness, pH, and alkalinity is greater than is typical for data in Table 1, the hardness equation based criteria may be overprotective. Appendix G shows representative water quality criteria values using both the BLM and the hardness equation approaches for waters with a range in pH, hardness, and DOC concentrations. The hardness approach does not consider pH and DOC while the BLM approach takes those water quality parameters into consideration.

4.2 Formulation of the CCC

4.2.1 Evaluation of Chronic Toxicity Data

In aquatic toxicity tests, chronic values are usually defined as the geometric mean of the highest concentration of a toxic substance at which no adverse effect is observed (highest no observed adverse effect concentration, or NOAEC) and the lowest concentration of the toxic substance that causes an adverse effect (lowest observed adverse effect concentration, or LOAEC). The significance of the observed effects is determined by statistical tests comparing responses of organisms exposed to low-level and control concentrations of the toxic substance against responses of organisms exposed to elevated concentrations. Analysis of variance is the most common test employed for such comparisons. This approach, however, has the disadvantage of resulting in marked differences between the magnitudes of the effects corresponding to the individual chronic values, because of variation in the power of the statistical tests used, the concentrations tested, and the size and variability of the samples used (Stephan and Rogers, 1985).

An alternative approach to calculating chronic values focuses on the use of point estimates such as from regression analysis to define the dose-response relationship. With a regression equation or probit analysis, which defines the level of adverse effects as a function of increasing concentrations of the toxic substance, it is possible to determine the concentration that causes a specific small effect, such as a 5 to 30 percent reduction in response. To make chronic values reflect a uniform level of effect, regression and probit analyses were used, where possible, both to demonstrate that a significant concentration-effect relationship was present and to estimate chronic

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values with a consistent level of effect. The most precise estimates of effect concentrations can generally be made for 50 percent reduction (EC50); however, such a major reduction is not necessarily consistent with criteria providing adequate protection. In contrast, a concentration that causes a low level of reduction, such as an EC5 or EC10, might not be statistically significantly different from the control treatment. As a compromise, the EC20 is used here to represent a low level of effect that is generally significantly different from the control treatment across the useful chronic datasets that are available for copper. The EC20 was also viewed as providing a level of protection similar to the geometric mean of the NOEC and LOEC. Since the EC20 is not directly dependent on the tested dilution series, similar EC20s should be expected irrespective of the tested concentrations, provided that the range of tested concentrations is appropriate.

Regression or probit analysis was utilized to evaluate a chronic dataset only in cases where the necessary data were available and the dataset met the following conditions: (1) it contained a control treatment (or low exposure data point) to anchor the curve at the low end, (2) it contained at least three concentrations, and (3) two of the data points had effect variable values below the control and above zero (i.e., “partial effects”). Control concentrations of copper were estimated in cases where no measurements were reported. These analyses were performed using the Toxicity Relationship Analysis Program software (version 1.0; U.S. EPA, Mid-Continental Ecology Division, Duluth, MN, USA). Additional detail regarding the aforementioned statistical procedures is available in the cited program.

When the data from an acceptable chronic test met the conditions for the logistic regression or probit analysis, the EC20 was the preferred chronic value. When data did not meet the conditions the chronic value was usually set to the geometric mean of the NOAEC and the LOAEC. However, when no treatment concentration was an NOAEC, the chronic value is reported as less than the

lowest tested concentration.

For life-cycle, partial life-cycle, and early life stage tests, the toxicological variable used in chronic value analyses was survival, reproduction, growth, emergence, or intrinsic growth rate. If copper apparently reduced both survival and growth (weight or length), the product of variables (biomass) was analyzed, rather than analyzing the variables separately. The most sensitive of the toxicological variables was generally selected as the chronic value for the particular study.

A species-by-species discussion of each acceptable chronic test on copper evaluated for this document is presented in Appendix F. Figures that present the data and regression/probability distribution line for each of the acceptable chronic test which contained sufficient acceptable data are also provided in Appendix F.

4.2.2 Calculation of Freshwater CCC

Acceptable freshwater chronic toxicity data from early life stage tests, partial life-cycle tests, and full life-cycle tests were available for 29 tests including data for 6 invertebrate species and 10 fish species (Table 2a). The 17 chronic values for invertebrate species range from 2.83 ( D. pulex) to 34.6 μg/L ( C. dubia ); and the 12 chronic values for the fish species range from <5 (brook trout) to 60.4 μg/L (northern pike). Of the 29 chronic tests, comparable acute values are available for 18 of the tests (Table 2c). The relationship between acute toxicity values and ACRs is presented in Figure

6. The supporting acute and chronic test values for the ACRs and the species mean ACRs are

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presented in Table 2c. For the 11 tests in Table 2a with chronic values both from a regression EC20 and the geometric mean of the NOAEC and LOAEC, the EC20 averaged 81% of the geometric mean, demonstrating the similar level of protection for the two approaches.

Overall, individual ACRs varied from <1 (0.55) for C. dubia (Oris et al., 1991) to 191.6 for the snail, Campeloma decisum (Arthur and Leonard, 1970). Species mean acute-chronic ratios ranged from 1.48 in saltwater for the sheepshead minnow (Hughes et al., 1989) to 171.2 in freshwater for the snail, C. decisum . Pursuant to the Guidelines (Stephan et al., 1985), consideration was given to calculating the FACR based on all ACRs within a factor of 10, but because there appeared to be a relationship between acute sensitivity and ACRs (Figure 6), the FACR was derived from data for species whose SMAVs were close to the FAV. The FACR of 3.22 was calculated as the geometric mean of the ACRs for sensitive freshwater species, C. dubia, D. magna, D. pulex, O. tshawytscha , and O. mykiss along with the one saltwater ACR for C. variegatus (Table 2b). Based on the normalization water chemistry conditions used for illustrative purposes in the document, the freshwater site specific FAV value is 4.67 μg/L, which divided by the FACR of 3.22 results in a freshwater FCV of 1.45 μg/L dissolved Cu.

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5.0 PLANT DATA

Copper has been widely used as an algicide and herbicide for nuisance aquatic plants (McKnight et al., 1983). Although copper is known as an inhibitor of photosynthesis and plant growth, toxicity data on individual species suitable for deriving aquatic life criteria (Table 4) are not

numerous.

The relationship of copper toxicity to the complexing capacity of the water or the culture medium is now widely recognized (Gächter et al., 1973; Petersen, 1982), and several studies have used algae to “assay” the copper complexing capacity of both fresh and salt waters (Allen et al., 1983; Lumsden and Florence, 1983; Rueter, 1983). It has also been shown that algae are capable of excreting complexing substances in response to copper stress (McKnight and Morel, 1979; Swallow et al., 1978; van den Berg et al., 1979). Foster (1982) and Stokes and Hutchinson (1976) have identified resistant strains and/or species of algae from copper (or other metal) impacted environments. A portion of this resistance probably results from induction of the chelate-excretion mechanism. Chelate excretion by algae may also serve as a protective mechanism for other aquatic organisms in eutrophic waters; that is, where algae are capable of maintaining free copper activities

below harmful concentrations.

Copper concentrations from 1 to 8,000 μg/L have been shown to inhibit growth of various freshwater plant species. Very few of these tests, though, were accompanied by analysis of actual copper exposure concentrations. Notable exceptions are freshwater tests with green alga including Chlamydomonas reinhardtii (Schafer et al., 1993; Winner and Owen, 1991b), which is the only flow-through, measured test with an aquatic plant, Chlorella vulgaris and Selenastrum capricornutum (Blaylock et al., 1985). There is also a measured test with duckweed, Lemna minor (Taraldsen and Norberg-King, 1990).

A direct comparison between the freshwater plant data and the BLM derived criteria is difficult to make without a better understanding of the composition of the algal media used for different studies (e.g., DOC, hardness, and pH) because these factors influence the applicable criteria comparison. BLM derived criteria for certain water conditions, such as low to mid-range pH, hardness up to 100 mg/L as CaCO 3 

# , and low DOC are in the range of, if not lower than, the lowest reported toxic endpoints for freshwater algal species and would therefore appear protective of plant species. In other water quality conditions BLM-derived criteria may be significantly higher (see Figure 5).

Two publications provide data for the red algae Champia parvula that indicate that reproduction of this species is especially sensitive to copper. The methods manual (U.S. EPA 1988) for whole effluent toxicity (WET) testing contains the results of six experiments showing nominal reproduction LOECs from 48-hr exposures to 1.0 to 2.5 μg/L copper (mean 2.0 μg/L); these tests used a mixture of 50 percent sterile seawater and 50 percent GP2 medium copper. The second study by Morrison et al. (1989) evaluated interlaboratory variation of the 48-hr WET test procedure; this six-test study gave growth EC50 values from 0.8 to 1.9 μg/L (mean 1.0 μg/L). Thus, there are actually 12 tests that provide evidence of significant reproductive impairment in C. parvula at nominal copper concentrations between 0.8 and 2.5 μg/L. For these studies though, the

dilution water source was not identified.

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One difficulty in assessing these data is the uncertainty of the copper concentration in the test solutions, primarily with respect to any background copper that might be found in the dilution water, especially with solutions compounded from sea salts or reagents. Thus, with a CCC of 1.9 μg/L dissolved copper, the significance of a 1 or 2 μg/L background copper level to a 1 to 3 μg/L

nominal effect level can be considerable.

The reproduction of other macroalgae appears to be generally sensitive to copper, but not to

the extent of Champia . Many of these other macroalgae appear to have greater ecological significance than Champia , several forming significant intertidal and subtidal habitats for other saltwater organisms, as well as being a major food source for grazers. Reproductive and growth effects on the other species of macroalgae sometimes appear to occur at copper concentrations between 5 and 10 μg/L (Appendix B, Other Data). Thus, most major macrophyte groups seem to be adequately protected by the CMC and CCC, but appear similar in sensitivity to some of the more sensitive groups of saltwater animals.

6.0 OTHER DATA

Many of the data identified for this effort are listed in Appendix B, Other Data, for various reasons, including exposure durations other than 96 hours with the same species reported in Table 1, and some exposures lasting up to 30 days. Acute values for test durations less than 96 hours are available for several species not shown in Table 1. Still, these species have approximately the same sensitivities to copper as species in the same families listed in Table 1. Reported LC50s at 200 hours for chinook salmon and rainbow trout (Chapman, 1978) differ only slightly from 96-hour LC50s reported for these same species in the same water.

A number of other acute tests in Appendix B were conducted in dilution waters that were not considered appropriate for criteria development. Brungs et al. (1976) and Geckler et al. (1976) conducted tests with many species in stream water that contained a large amount of effluent from a sewage treatment plant. Wallen et al. (1957) tested mosquitofish in a turbid pond water. Until chemical measurements that correlate well with the toxicity of copper in a wide variety of waters are identified and widely used, results of tests in unusual dilution waters, such as those in Appendix B, will not be very useful for deriving water quality criteria.

Appendix B also includes tests based on physiological effects, such as changes in appetite, blood parameters, stamina, etc. These were included in Appendix B because they could not be directly interpreted for derivation of criteria. For the reasons stated in this section above, data in Appendix B was not used for criteria derivation.

A direct comparison of a particular test result to a BLM-derived criterion is not always straightforward, particularly if complete chemical characterization of the test water is not available. Such is the case for a number of studies included in Appendix B. While there are some test results with effect concentrations below the example criteria concentrations presented in this document, these same effect concentrations could be above criteria derived for other normalization chemistries, raising the question as to what is the appropriate comparison to make. For example, Appendix B

includes an EC50 for D. Pulex of 3.6 μg/L (Koivisto et al., 1992) at an approximate hardness of 25 mg/L (33 mg/L as CaCO 3 

# ). Yet, example criteria at a hardness of 25 mg/L (as CaCO 3 

# ) (including those in Figure 6) range from 0.23 μg/L (DOC = 0.1 mg/L) to 4.09 μg/L (DOC = 2.3 mg/L) based

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on the DOC concentration selected for the synthetic water recipe. The chemical composition for the Koivisto et al. (1992) study would dictate what the appropriate BLM criteria comparison should be.

Based on the expectation that many of the test results presented in Appendix B were conducted in laboratory dilution water with low levels of DOC, the appropriate comparison would be to the criteria derived from low DOC waters. Comparing many of the values in Appendix B to the example criteria presented in this document, it appears that a large proportion of Appendix B values are above these concentration levels. This is a broad generalization though and as stated previously, all important water chemistry variables that affect toxicity of copper to aquatic organisms should be considered before making these types of comparisons.

Studies not considered suitable for criteria development were placed in Appendix G, Unused

Data.

7.0 NATIONAL CRITERIA STATEMENT

The available toxicity data, when evaluated using the procedures described in the “Guidelines for Deriving Numerical National Water Quality Criteria for the Protection of Aquatic Organisms and Their Uses” indicate that freshwater aquatic life should be protected if the 24-hour average and four-day average concentrations do not respectively exceed the acute and chronic criteria concentrations calculated by the Biotic Ligand Model.

A return interval of 3 years between exceedances of the criterion continues to be EPA's general recommendation. However, the resilience of ecosystems and their ability to recover differ greatly. Therefore, scientific derivation of alternative frequencies for exceeding criteria may be appropriate.

8.0 IMPLEMENTATION

The use of water quality criteria in designing waste treatment facilities and appropriate effluent limits involves the use of an appropriate wasteload allocation model. Although dynamic models are preferred for application of these criteria, limited data or other factors may make their use impractical, in which case one should rely on a steady-state model. EPA recommends the interim use of 1B3 or 1Q10 for criterion maximum concentration stream design flow and 4B3 or 7Q10 for the criterion continuous concentration design flow in steady-state models. These matters are discussed in more detail in the Technical Support Document for Water Quality-Based Toxics Control (U.S. EPA, 1991).

With regard to BLM-derived freshwater criteria, to develop a site-specific criterion for a stream reach, one is faced with determining what single criterion is appropriate even though a BLM criterion calculated for the event corresponding to the input water chemistry conditions will be time-variable. This is not a new problem unique to the BLM—hardness-dependent metals criteria are also time-variable values. Although the variability of hardness over time can be characterized, EPA has not provided guidance on how to calculate site-specific criteria considering this variability. Multiple input parameters for the BLM could complicate the calculation of site-specific criteria because of their combined effects on variability. Another problem arise from potential scarcity of data from small stream reaches with small dischargers. The EPA is currently exploring two

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approaches to fill data gaps in such situations. One potential approach is the selection of values based on geography, the second approach is based on correlations between measured parameters and missing parameter measurements. A companion document in the form of Supplementary Training Materials, addressing issues related to data requirements, implementation, permitting, and monitoring will be released via EPA's website following the publication of

this criteria document.Table 1. Acute Toxicity of Copper to Freshwater AnimalsSpeciesaOrganism Age,Size, or LifestageMethodbChemicalcReported LC50 orEC50(total μg/L)dReported LC50or EC50(Diss. μg/L)eBLM Data LabelBLM NormalizedLC50 or EC50(μg/L)fSpecies Mean AcuteValue (μg/L)gReferenceWorm, adult (mixed age) S,M,T N 130 --- LUVA01S 37.81 48.41 Schubauer-Berigan et al. 1993Lumbriculus variegatadult(mixed age)S,M,T N270---LUVA02S55.39Schubauer-Berigan et al. 1993adult(mixed age)S,M,T N500---LUVA03S54.18Schubauer-Berigan et al. 1993Snail,1.1-2.7 cm F,M,TS2000---CADE01F43193573 Arthur and Leonard 1970Campeloma1.1-2.7 cm F,M,TS1400---CADE02F2956Arthur and Leonard 1970Snail,Juga pliciferaadultF,M,T C15---JUPL01F12.3112.31 Nebeker et al. 1986bSnail,Lithoglyphus virensadultF,M,T C8---LIVI01F6.676.67 Nebeker et al. 1986bSnail,0.4-0.7 cm F,M,TS41---PHIN01F21.8120.41 Arthur and Leonard 1970Physa integra0.4-0.7 cm F,M,T S 37 --- PHIN02F 19.09 Arthur and Leonard 1970Freshwater mussel, juvenile S,M,T S 27 --- ACPE01S 10.36 11.33 Keller unpublishedActinonaiasjuvenile S,M,T S <29 --- ACPE02S 12.39 Keller unpublishedFreshwater mussel, 1-2 d juv S,M,T S 86 --- UTIM01S 177.9 52.51 Keller and Zam 1991Utterbackia imbecillis1-2 d juv S,M,T S199---UTIM02S172.3Keller and Zam 1991juvenileS,M,T N76---UTIM03S40.96Keller unpublishedjuvenileS,M,T N85---UTIM04S43.22Keller unpublishedjuvenileS,M,T N41---UTIM05S24.12Keller unpublishedjuvenileS,M,T S79---UTIM06S39.04Keller unpublishedjuvenileS,M,T S72---UTIM07S39.96Keller unpublishedjuvenileS,M,T S38---UTIM08S28.31Keller unpublishedCladoceran,<4 hS,M,T C19---CEDU01S10.285.93 Carlson et al. 1986Ceriodaphnia dubia<4 hS,M,T C17---CEDU02S9.19Carlson et al. 1986<12 hS,M,D ----25CEDU03S7.98Belanger et al. 1989<12 hS,M,D ----17CEDU04S5.25Belanger et al. 1989<12 hS,M,D ----30CEDU05S9.80Belanger et al. 1989<12 hS,M,D ----24CEDU06S7.63Belanger et al. 1989<12 hS,M,D ----28CEDU07S9.06Belanger et al. 1989<12 hS,M,D ----32CEDU08S10.56Belanger et al. 1989<12 hS,M,D ----23CEDU09S7.28Belanger et al. 1989<12 hS,M,D ----20CEDU10S6.25Belanger et al. 1989<12 hS,M,D ----19CEDU11S5.91Belanger et al. 1989<12 hS,M,D ----26CEDU12S3.10Belanger et al. 1989<12 hS,M,D ----21CEDU13S2.46Belanger et al. 1989<12 hS,M,D ----27CEDU14S3.24Belanger et al. 1989<12 hS,M,D ----37CEDU15S4.66Belanger et al. 1989<12 hS,M,D ----34CEDU16S4.22Belanger et al. 1989<12 hS,M,D ----67CEDU17S5.50Belanger et al. 1989<12 hS,M,D ----38CEDU18S2.72Belanger et al. 1989<12 hS,M,D ----78CEDU19S6.74Belanger et al. 1989<12 hS,M,D ----81CEDU20S7.10Belanger et al. 1989<12 hS,M,D ----28CEDU21S4.10Belanger and Cherry 199024Table 1. Acute Toxicity of Copper to Freshwater AnimalsSpeciesaOrganism Age,Size, or LifestageMethodbChemicalcReported LC50 orEC50(total μg/L)dReported LC50or EC50(Diss. μg/L)eBLM Data LabelBLM NormalizedLC50 or EC50(μg/L)fSpecies Mean AcuteValue (μg/L)gReference<12 hS,M,D ----84CEDU22S10.74Belanger and Cherry 1990<12 h S,M,T S 13.4 --- CEDU23S 6.19 Oris et al. 1991<24 h R,M,T,D S 6.98 5.54 CEDU24R 5.03 Diamond et al. 1997bCladoceran, 1 d S,M,T C 9.1 --- DAMA01S 3.42 6.00 Nebeker et al. 1986aDaphnia magna1 d S,M,T C 11.7 --- DAMA02S 4.43 Nebeker et al. 1986a<2 h S,M,T C 6.6 --- DAMA03S 2.50 Nebeker et al. 1986a<2 h S,M,T C 9.9 --- DAMA04S 3.78 Nebeker et al. 1986a1 d S,M,T C 11.7 --- DAMA05S 13.46 Nebeker et al. 1986a<4 h S,M,T C 6.7 --- DAMA06S 8.21 Nebeker et al. 1986a1 d S,M,T C 9.1 --- DAMA07S 4.40 Nebeker et al. 1986a<2 h S,M,T C 5.2 --- DAMA08S 2.16 Nebeker et al. 1986a<24 h S,M,T S 41.2 --- DAMA09S 21.55 Baird et al. 1991<24 h S,M,T S 10.5 --- DAMA10S 5.63 Baird et al. 1991<24 h S,M,T S 20.6 --- DAMA11S 11.31 Baird et al. 1991<24 h S,M,T S 17.3 --- DAMA12S 9.48 Baird et al. 1991<24 h S,M,T S 70.7 --- DAMA13S 33.58 Baird et al. 1991<24 h S,M,T S 31.3 --- DAMA14S 16.90 Baird et al. 1991<24 h S,M,I S 7.1 --- DAMA15S 2.67 Meador 1991<24 h S,M,I S 16.4 --- DAMA16S 4.26 Meador 1991<24 h S,M,I S 39.9 --- DAMA17S 5.18 Meador 1991<24 h S,M,I S 18.7 --- DAMA18S 3.39 Meador 1991<24 h S,M,I S 18.9 --- DAMA19S 1.99 Meador 1991<24 h S,M,I S 39.7 --- DAMA20S 3.04 Meador 1991<24 h S,M,I S 46 --- DAMA21S 8.93 Meador 1991<24 h S,M,I S 71.9 --- DAMA22S 9.97 Meador 1991<24 h S,M,I S 57.2 --- DAMA23S 5.76 Meador 1991<24 h S,M,I S 67.8 --- DAMA24S 4.16 Meador 1991<24 h S,M,T C 26 --- DAMA25S 10.34 Chapman et al. Manuscript<24 h S,M,T C 30 --- DAMA26S 9.04 Chapman et al. Manuscript<24 h S,M,T C 38 --- DAMA27S 9.84 Chapman et al. Manuscript<24 h S,M,T C 69 --- DAMA28S 12.31 Chapman et al. Manuscript<24 h S,M,T,D S 4.8 --- DAMA29S 1.22 Long's MS Thesis<24 h S,M,T,D S 7.4 --- DAMA30S 16.29 Long's MS Thesis<24 h S,M,T,D S 6.5 --- DAMA31S 2.11 Long's MS ThesisCladoceran, --- S,M,T S 11.4 --- DAPC01S 1.63 2.73 Lind et al. Manuscript (1978)Daphnia pulicaria--- S,M,T S 9.06 --- DAPC02S 1.04 Lind et al. Manuscript (1978)--- S,M,T S 7.24 --- DAPC03S 0.88 Lind et al. Manuscript (1978)--- S,M,T S 10.8 --- DAPC04S 1.13 Lind et al. Manuscript (1978)--- S,M,T S 55.4 --- DAPC05S 8.81 Lind et al. Manuscript (1978)--- S,M,T S 55.3 --- DAPC06S 6.03 Lind et al. Manuscript (1978)--- S,M,T S 53.3 --- DAPC07S 4.12 Lind et al. Manuscript (1978)--- S,M,T S 97.2 --- DAPC08S 3.94 Lind et al. Manuscript (1978)25Table 1. Acute Toxicity of Copper to Freshwater AnimalsSpeciesaOrganism Age,Size, or LifestageMethodbChemicalcReported LC50 orEC50(total μg/L)dReported LC50or EC50(Diss. μg/L)eBLM Data LabelBLM NormalizedLC50 or EC50(μg/L)fSpecies Mean AcuteValue (μg/L)gReference---S,M,T S199---DAPC09S3.01Lind et al. Manuscript (1978)--- S,M,T S 213 --- DAPC10S 7.63 Lind et al. Manuscript (1978)--- S,M,T S 165 --- DAPC11S 5.78 Lind et al. Manuscript (1978)--- S,M,T S 35.5 --- DAPC12S 1.83 Lind et al. Manuscript (1978)--- S,M,T S 78.8 --- DAPC13S 2.36 Lind et al. Manuscript (1978)--- S,M,T S 113 --- DAPC14S 1.06 Lind et al. Manuscript (1978)--- S,M,T S 76.4 --- DAPC15S 2.36 Lind et al. Manuscript (1978)--- S,M,T S 84.7 --- DAPC16S 6.62 Lind et al. Manuscript (1978)--- S,M,T S 184 --- DAPC17S 7.14 Lind et al. Manuscript (1978)--- S,M,T S 9.3 --- DAPC18S 1.11 Lind et al. Manuscript (1978)--- S,M,T S 17.8 --- DAPC19S 2.11 Lind et al. Manuscript (1978)--- S,M,T S 23.7 --- DAPC20S 2.67 Lind et al. Manuscript (1978)--- S,M,T S 27.3 --- DAPC21S 2.77 Lind et al. Manuscript (1978)--- S,M,T S 25.2 --- DAPC22S 2.81 Lind et al. Manuscript (1978)--- S,M,T S 25.1 --- DAPC23S 2.60 Lind et al. Manuscript (1978)--- S,M,T S 25.1 --- DAPC24S 2.31 Lind et al. Manuscript (1978)Cladoceran,Scapholeberis sp.adult S,M,T C 18 --- SCSP01S 9.73 9.73 Carlson et al. 1986Amphipod, 1-3 d F,M,T S 22 --- GAPS01F 10.39 9.60 Arthur and Leonard 1970Gammarus1-3 d F,M,T S 19 --- GAPS02F 8.86 Arthur and Leonard 1970Amphipod, 7-14 d S,M,T N 17 --- HYAZ01S 12.19 12.07 Schubauer-Berigan et al. 1993Hyalella azteca7-14 dS,M,T N24---HYAZ02S9.96Schubauer-Berigan et al. 19937-14 dS,M,T N87---HYAZ03S15.77Schubauer-Berigan et al. 1993<7 dS,M,T S24.3---HYAZ04S8.26Welsh 1996<7 d S,M,T S 23.8 --- HYAZ05S 8.09 Welsh 1996<7 d S,M,T S 8.2 --- HYAZ06S 15.49 Welsh 1996<7 d S,M,T S 10 --- HYAZ07S 18.80 Welsh 1996Stonefly,Acroneuria lycorias--- S,M,T S 8300 --- ACLY01S 20636 20636 Warnick and Bell 1969Midge,Chironomus4th instar S,M,T S 739 --- CHDE01S 1987 1987 Kosalwat and Knight 1987Shovelnosesturgeon,Scaphirhynchusfry, 6.01 cm, 0.719 g S,M,T S160---SCPL01S69.6369.63 Dwyer et al. 1999Apache trout,Oncorhynchuslarval, 0.38 g S,M,T S 70 --- ONAP01S 32.54 32.54 Dwyer et al. 1995Lahontan cutthroat larval, 0.34 g S,M,T S 80 --- ONCL01S 34.26 32.97 Dwyer et al. 1995Oncorhynchusclarki henshawilarval, 0.57 g S,M,T S 60 --- ONCL02S 24.73 Dwyer et al. 199526Table 1. Acute Toxicity of Copper to Freshwater AnimalsSpeciesaOrganism Age,Size, or LifestageMethodbChemicalcReported LC50 orEC50(total μg/L)dReported LC50or EC50(Diss. μg/L)eBLM Data LabelBLM NormalizedLC50 or EC50(μg/L)fSpecies Mean AcuteValue (μg/L)gReferenceCutthroat trout, 7.4 cm, 4.2 g F,M,T,D C398.91367ONCL03F67.30Chakoumakos et al. 1979Oncorhynchus clarki6.9 cm, 3.2 g F,M,T,D C197.87186ONCL04F44.91Chakoumakos et al. 19798.8 cm, 9.7 g F,M,T,D C 41.35 36.8 ONCL05F 21.87 Chakoumakos et al. 19798.1 cm, 4.4 g F,M,T,D C 282.93 232 ONCL06F 51.94 Chakoumakos et al. 19796.8 cm, 2.7 g F,M,T,D C 186.21 162 ONCL07F 111.3 Chakoumakos et al. 19797.0 cm, 3.2 g F,M,T,D C 85.58 73.6 ONCL08F 39.53 Chakoumakos et al. 19798.5 cm, 5.2 g F,M,T,D C 116.67 91 ONCL09F 19.63 Chakoumakos et al. 19797.7 cm, 4.4 g F,M,T,D C 56.20 44.4 ONCL10F 18.81 Chakoumakos et al. 19798.9 cm, 5.7 g F,M,T,D C 21.22 15.7 ONCL11F 10.60 Chakoumakos et al. 1979Pink salmon,alevin (newly hatched)F,M,TS143---ONGO01F41.6540.13 Servizi and Martens 1978Oncorhynchus gorbualevinF,M,TS87---ONGO02F19.70Servizi and Martens 1978fryF,M,TS199---ONGO03F78.76Servizi and Martens 1978Coho salmon,6 gR,M,T,I ---164---ONKI01R106.0922.93 Buckley 1983Oncorhynchus kisutcparrF,M,T C33---ONKI02F20.94Chapman 1975adult, 2.7 kg F,M,T C46---ONKI03F32.66Chapman and Stevens 1978fryF,M,T,D,I ---6149ONKI04F12.67Mudge et al. 1993smolt F,M,T,D,I ---6351ONKI05F13.19Mudge et al. 1993fryF,M,T,D,I ---8658ONKI06F11.95Mudge et al. 1993parrF,M,T,D,I ---10378ONKI07F22.98Mudge et al. 1993Rainbow trout, larval, 0.67 g S,M,T S110---ONMY01S41.6422.19 Dwyer et al. 1995Oncorhynchus mykislarval, 0.48 g S,M,T S50---ONMY02S25.26Dwyer et al. 1995larval, 0.50 g S,M,T S60---ONMY03S29.46Dwyer et al. 1995swim-up, 0.25 g R,M,T,D C46.740ONMY04R10.90Cacela et al. 1996swim-up, 0.25 g R,M,T,D C24.219ONMY05R9.04Cacela et al. 1996swim-up, 0.20-0.24 g R,M,T,D C03.4ONMY06R5.02Welsh et al. 2000swim-up, 0.20-0.24 g R,M,T,D C08.1ONMY07R11.97Welsh et al. 2000swim-up, 0.20-0.24 g R,M,T,D C017.2ONMY08R13.80Welsh et al. 2000swim-up, 0.20-0.24 g R,M,T,D C032ONMY09R23.84Welsh et al. 2000alevinF,M,T C28---ONMY10F20.30Chapman 1975, 1978swim-up, 0.17 g F,M,T C17---ONMY11F12.54Chapman 1975, 1978parr, 8.6 cm, 6.96 g F,M,T C18---ONMY12F9.87Chapman 1975, 1978smolt, 18.8 cm, 68.19gF,M,T C29---ONMY13F22.48Chapman 1975, 19781 gF,M,T,D C-169ONMY14F23.41Chakoumakos et al. 19794.9 cm F,M,T,D C-85.3ONMY15F10.20Chakoumakos et al. 19796.0 cm, 2.1 g F,M,T,D C-83.3ONMY16F9.93Chakoumakos et al. 19796.1 cm, 2.5 g F,M,T,D C-103ONMY17F12.71Chakoumakos et al. 19792.6 gF,M,T,D C-274ONMY18F44.54Chakoumakos et al. 19794.3 gF,M,T,D C-128ONMY19F16.51Chakoumakos et al. 19799.2 cm, 9.4 g F,M,T,D C-221ONMY20F33.33Chakoumakos et al. 19799.9 cm, 11.5 g F,M,T,D C-165ONMY21F22.70Chakoumakos et al. 197911.8 cm, 18.7 g F,M,T,D C-197ONMY22F28.60Chakoumakos et al. 197913.5 cm, 24.9 g F,M,T,D C-514ONMY23F99.97Chakoumakos et al. 197927Table 1. Acute Toxicity of Copper to Freshwater AnimalsSpeciesaOrganism Age,Size, or LifestageMethodbChemicalcReported LC50 orEC50(total μg/L)dReported LC50or EC50(Diss. μg/L)eBLM Data LabelBLM NormalizedLC50 or EC50(μg/L)fSpecies Mean AcuteValue (μg/L)gReference13.4 cm, 25.6 g F,M,T,D C-243ONMY24F37.88Chakoumakos et al. 19796.7 cm, 2.65 g F,M,T C2.8---ONMY25F7.00Cusimano et al. 1986parrF,M,T,D,I ---9068ONMY26F19.73Mudge et al. 1993swim-up, 0.29 g F,M,T,D C19.618ONMY27F8.10Cacela et al. 1996swim-up, 0.25 g F,M,T,D C12.912ONMY28F32.15Cacela et al. 1996swim-up, 0.23 g F,M,T,D C5.95.7ONMY29F24.80Cacela et al. 1996swim-up, 0.23 g F,M,T,D C37.835ONMY30F16.16Cacela et al. 1996swim-up, 0.26 g F,M,T,D C25.118ONMY31F37.66Cacela et al. 1996swim-up, 0.23 g F,M,T,D C17.217ONMY32F24.19Cacela et al. 19960.64 g, 4.1 cm F,M,T,D C101---ONMY33F39.73Hansen et al. 20000.35 g, 3.4 cm F,M,T,D C308---ONMY34F85.83Hansen et al. 20000.68 g, 4.2 cm F,M,T,D C93---ONMY35F95.9Hansen et al. 20000.43 g, 3.7 cm F,M,T,D C35.9---ONMY36F50.83Hansen et al. 20000.29 g, 3.4 cm F,M,T,D C54.4---ONMY37F47.69Hansen et al. 2000Sockeye salmon,alevin (newly hatched)F,M,TS190---ONNE01F71.7354.82 Servizi and Martens 1978Oncorhynchus nerkaalevinF,M,TS200---ONNE02F79.52Servizi and Martens 1978alevinF,M,TS100---ONNE03F23.74Servizi and Martens 1978alevinF,M,TS110---ONNE04F27.22Servizi and Martens 1978alevinF,M,TS130---ONNE05F35.36Servizi and Martens 1978fryF,M,TS150---ONNE06F45.37Servizi and Martens 1978smolt, 5.5 g F,M,TS210---ONNE07F87.77Servizi and Martens 1978smolt, 5.5 g F,M,TS170---ONNE08F57.53Servizi and Martens 1978smolt, 5.5 g F,M,TS190---ONNE09F71.73Servizi and Martens 1978smolt, 4,8 g F,M,TS240---ONNE10F114.4Servizi and Martens 1978Chinook salmon, alevin, 0.05 g F,M,T C26---ONTS01F14.4825.02 Chapman 1975, 1978Oncorhynchus tshawswim-up, 0.23 g F,M,T C19---ONTS02F10.44Chapman 1975, 1978parr, 9.6 cm, 11.58 g F,M,T C38---ONTS03F28.30Chapman 1975, 1978smolt, 14.4 cm, 32.46gF,M,T C26---ONTS04F20.09Chapman 1975, 19783 mo, 1.35 g F,M,T,I C10.2---ONTS05F19.41Chapman and McCrady 19773 mo, 1.35 g F,M,T,I C24.1---ONTS06F30.91Chapman and McCrady 19773 mo, 1.35 g F,M,T,I C82.5---ONTS07F32.74Chapman and McCrady 19773 mo, 1.35 g F,M,T,I C128.4---ONTS08F20.66Chapman and McCrady 1977swim-up, 0.36-0.45 g F,M,T,D C07.4ONTS09F36.49Welsh et al. 2000swim-up, 0.36-0.45 g F,M,T,D C012.5ONTS10F30.85Welsh et al. 2000swim-up, 0.36-0.45 g F,M,T,D C014.3ONTS11F31.49Welsh et al. 2000swim-up, 0.36-0.45 g F,M,T,D C018.3ONTS12F48.56Welsh et al. 200028Table 1. Acute Toxicity of Copper to Freshwater AnimalsSpeciesaOrganism Age,Size, or LifestageMethodbChemicalcReported LC50 orEC50(total μg/L)dReported LC50or EC50(Diss. μg/L)eBLM Data LabelBLM NormalizedLC50 or EC50(μg/L)fSpecies Mean AcuteValue (μg/L)gReferenceBull trout, 0.130 g, 2.6 cm F,M,T,D C228---SACO01F69.7068.31 Hansen et al. 2000Salvelinus confluentu0.555 g, 4.0 cm F,M,T,D C207---SACO02F63.62Hansen et al. 20000.774 g, 4.5 cm F,M,T,D C66.6---SACO03F74.18Hansen et al. 20001.520 g, 5.6 cm F,M,T,D C50---SACO04F63.60Hansen et al. 20001.160 g, 5.2 cm F,M,T,D C89---SACO05F71.11Hansen et al. 2000Chiselmouth,Acrocheilus4.6 cm, 1.25 g F,M,T C143---ACAL01F216.3216.3 Andros and Garton 1980Bonytail chub,Gila eleganslarval, 0.29 g S,M,T S200---GIEL01S63.2263.22 Dwyer et al. 1995Golden shiner,Notemigonuscrysoleucas---F,M,T C84600---NOCR01F107860107860 Hartwell et al. 1989Fathead minnow, adult, 40 mm S,M,T S310---PIPR01S266.369.63 Birge et al. 1983Pimephales promelaadult, 40 mm S,M,T S120---PIPR02S105.61Birge et al. 1983adult, 40 mm S,M,T S390---PIPR03S207.3Birge et al. 1983; Benson & Birge--- S,M,T C 55 --- PIPR04S 38.08 Carlson et al. 1986--- S,M,T C 85 --- PIPR05S 70.71 Carlson et al. 1986<24 h S,M,T N 15 --- PIPR06S 11.23 Schubauer-Berigan et al. 1993<24 hS,M,T N44---PIPR07S18.03Schubauer-Berigan et al. 1993<24 hS,M,T N>200---PIPR08S24.38Schubauer-Berigan et al. 1993<24 h, 0.68 mg S,M,T S4.82---PIPR09S8.87Welsh et al. 1993<24 h, 0.68 mg S,M,T S8.2---PIPR10S16.72Welsh et al. 1993<24 h, 0.68 mg S,M,T S31.57---PIPR11S25.15Welsh et al. 1993<24 h, 0.68 mg S,M,T S21.06---PIPR12S17.67Welsh et al. 1993<24 h, 0.68 mg S,M,T S35.97---PIPR13S21.24Welsh et al. 1993<24 h, 0.68 mg S,M,T S59.83---PIPR14S16.64Welsh et al. 1993<24 h, 0.68 mg S,M,T S4.83---PIPR15S5.92Welsh et al. 1993<24 h, 0.68 mg S,M,T S70.28---PIPR16S13.34Welsh et al. 1993<24 h, 0.68 mg S,M,T S83.59---PIPR17S8.22Welsh et al. 1993<24 h, 0.68 mg S,M,T S182---PIPR18S13.91Welsh et al. 1993larval, 0.32 g S,M,T S290---PIPR19S73.92Dwyer et al. 1995larval, 0.56 g S,M,T S630---PIPR20S157.9Dwyer et al. 1995larval, 0.45 g S,M,T S400---PIPR21S103.2Dwyer et al. 1995larval, 0.39 g S,M,T S390---PIPR22S161.7Dwyer et al. 19953.2-5.5 cm, 0.42-3.23gS,M,T S450---PIPR23S152.9Richards and Beitinger 19952.8-5.1 cm, 0.30-2.38gS,M,T S297---PIPR24S77.75Richards and Beitinger 19951.9-4.6 cm, 0.13-1.55gS,M,T S311---PIPR25S67.56Richards and Beitinger 19953.0-4.8 cm, 0.23-1.36gS,M,T S513---PIPR26S76.36Richards and Beitinger 1995<24 h S,M,T,D S62.2353.96PIPR27S25.70Erickson et al. 1996a,b<24 h S,M,T,D S190.5165.18PIPR28S87.89Erickson et al. 1996a,b<24 h S,M,T,D S68.5859.46PIPR29S28.59Erickson et al. 1996a,b<24 h S,M,T,D S168.91146.46PIPR30S89.18Erickson et al. 1996a,b29Table 1. Acute Toxicity of Copper to Freshwater AnimalsSpeciesaOrganism Age,Size, or LifestageMethodbChemicalcReported LC50 orEC50(total μg/L)dReported LC50or EC50(Diss. μg/L)eBLM Data LabelBLM NormalizedLC50 or EC50(μg/L)fSpecies Mean AcuteValue (μg/L)gReference<24 h S,M,T,D S94.6282.04PIPR31S49.27Erickson et al. 1996a,b<24 h S,M,T,D S 143.51 124.43 PIPR32S 104.90 Erickson et al. 1996a,b<24 h S,M,T,D S 120.65 103.76 PIPR33S 86.54 Erickson et al. 1996a,b<24 h S,M,T,D S 196.85 167.32 PIPR34S 122.0 Erickson et al. 1996a,b<24 h S,M,T,D S 133.35 120.02 PIPR35S 75.0 Erickson et al. 1996a,b<24 h S,M,T,D S 184.15 169.42 PIPR36S 122.2 Erickson et al. 1996a,b<24 h S,M,T,D S 304.8 268.22 PIPR37S 78.5 Erickson et al. 1996a,b<24 h S,M,T,D S 292.1 242.44 PIPR38S 201.5 Erickson et al. 1996a,b<24 h S,M,T,D S 133.35 113.35 PIPR39S 100.75 Erickson et al. 1996a,b<24 h S,M,T,D S 92.71 77.88 PIPR40S 72.95 Erickson et al. 1996a,b<24 h S,M,T,D S 152.4 128.02 PIPR41S 112.9 Erickson et al. 1996a,b<24 h S,M,T,D S 177.8 151.13 PIPR42S 136.3 Erickson et al. 1996a,b<24 h S,M,T,D S 203.2 166.62 PIPR43S 136.0 Erickson et al. 1996a,b<24 h S,M,T,D S 190.5 163.83 PIPR44S 147.7 Erickson et al. 1996a,b<24 h S,M,T,D S 196.85 157.48 PIPR45S 125.9 Erickson et al. 1996a,b<24 h S,M,T,D S 234.95 199.71 PIPR46S 157.4 Erickson et al. 1996a,b<24 h S,M,T,D S 146.05 128.52 PIPR47S 127.8 Erickson et al. 1996a,b<24 h S,M,T,D S 171.45 150.88 PIPR48S 153.9 Erickson et al. 1996a,b<24 h S,M,T,D S 152.4 131.06 PIPR49S 114.57 Erickson et al. 1996a,b<24 h S,M,T,D S 184.15 160.21 PIPR50S 131.3 Erickson et al. 1996a,b<24 h S,M,T,D S 203.2 182.88 PIPR51S 130.9 Erickson et al. 1996a,b<24 h S,M,T,D S 203.2 180.85 PIPR52S 105.76 Erickson et al. 1996a,b<24 h S,M,T,D S 203.2 176.78 PIPR53S 128.8 Erickson et al. 1996a,b<24 h S,M,T,D S 222.25 188.91 PIPR54S 122.1 Erickson et al. 1996a,b<24 h S,M,T,D S 146.05 125.60 PIPR55S 111.87 Erickson et al. 1996a,b<24 h S,M,T,D S 139.7 117.35 PIPR56S 85.45 Erickson et al. 1996a,b<24 h S,M,T,D S 139.7 114.55 PIPR57S 83.10 Erickson et al. 1996a,b<24 h S,M,T,D S 152.4 126.49 PIPR58S 85.82 Erickson et al. 1996a,b<24 h S,M,T,D S 203.2 172.72 PIPR59S 110.0 Erickson et al. 1996a,b<24 h S,M,T,D S 196.85 167.32 PIPR60S 106.46 Erickson et al. 1996a,b<24 h S,M,T,D S 266.7 226.70 PIPR61S 133.4 Erickson et al. 1996a,b<24 h S,M,T,D S 99.06 84.20 PIPR62S 138.0 Erickson et al. 1996a,b<24 h S,M,T,D S 111.13 97.79 PIPR63S 165.8 Erickson et al. 1996a,b<24 h S,M,T,D S 78.74 70.08 PIPR64S 114.8 Erickson et al. 1996a,b<24 h S,M,T,D S 92.71 81.58 PIPR65S 121.5 Erickson et al. 1996a,b<24 h S,M,T,D S 85.09 77.43 PIPR66S 106.69 Erickson et al. 1996a,b<24 h S,M,T,D S 123.19 110.87 PIPR67S 124.7 Erickson et al. 1996a,b<24 h S,M,T,D S 165.1 151.89 PIPR68S 114.24 Erickson et al. 1996a,b<24 h S,M,T,D S 190.5 175.26 PIPR69S 89.93 Erickson et al. 1996a,b<24 h S,M,T,D S 165.1 145.29 PIPR70S 140.2 Erickson et al. 1996a,b<24 h S,M,T,D S 127 111.76 PIPR71S 100.16 Erickson et al. 1996a,b<24 h S,M,T,D S 92.08 79.18 PIPR72S 58.74 Erickson et al. 1996a,b30Table 1. Acute Toxicity of Copper to Freshwater AnimalsSpeciesaOrganism Age,Size, or LifestageMethodbChemicalcReported LC50 orEC50(total μg/L)dReported LC50or EC50(Diss. μg/L)eBLM Data LabelBLM NormalizedLC50 or EC50(μg/L)fSpecies Mean AcuteValue (μg/L)gReference<24 h S,M,T,D S66.6860.01PIPR73S37.67Erickson et al. 1996a,b<24 h S,M,T,D S 393.70 370.08 PIPR74S 163.3 Erickson et al. 1996a,b<24 h S,M,T,D S 317.50 292.10 PIPR75S 252.2 Erickson et al. 1996a,b<24 h S,M,T,D S 107.95 101.47 PIPR76S 169.6 Erickson et al. 1996a,b<24 h S,M,T,D S 67.95 62.51 PIPR77S 146.5 Erickson et al. 1996a,b<24 h S,M,T,D S 45.72 42.06 PIPR78S 126.3 Erickson et al. 1996a,b<24 h S,M,T,D S 177.80 172.47 PIPR79S 197.6 Erickson et al. 1996a,b<24 h S,M,T,D S 13.97 12.43 PIPR80S 28.13 Erickson et al. 1996a,b<24 h S,M,T,D S 304.80 271.27 PIPR81S 149.2 Erickson et al. 1996a,b<24 h S,M,T,D S 71.12 71.12 PIPR82S 105.76 Erickson et al. 1996a,b<24 h S,M,T,D S 83.82 79.63 PIPR83S 108.41 Erickson et al. 1996a,b<24 h S,M,T,D S 104.78 99.54 PIPR84S 114.7 Erickson et al. 1996a,b<24 h S,M,T,D S 139.70 132.72 PIPR85S 137.8 Erickson et al. 1996a,b<24 h S,M,T,D S 152.40 137.16 PIPR86S 114.8 Erickson et al. 1996a,b<24 h S,M,T,D S 260.35 182.25 PIPR87S 114.8 Erickson et al. 1996a,b<24 h S,M,T,D S 488.95 268.92 PIPR88S 122.1 Erickson et al. 1996a,b<24 h S,M,T,D S 203.20 188.98 PIPR89S 147.5 Erickson et al. 1996a,b<24 h S,M,T,D S 704.85 662.56 PIPR90S 185.0 Erickson et al. 1996a,b<24 h S,M,T,D S 952.50 904.88 PIPR91S 197.1 Erickson et al. 1996a,b<24 h S,M,T,D S 1244.60 995.68 PIPR92S 188.3 Erickson et al. 1996a,b<24 h S,M,T,D S 1485.90 891.54 PIPR93S 135.5 Erickson et al. 1996a,b<24 h S,M,T,D S 781.05 757.62 PIPR94S 181.4 Erickson et al. 1996a,b<24 h S,M,T,D S 476.25 404.81 PIPR95S 172.5 Erickson et al. 1996a,b<24 h S,M,T,D S 273.05 262.13 PIPR96S 191.4 Erickson et al. 1996a,b<24 h S,M,T,D S 22.23 20.45 PIPR97S 59.14 Erickson et al. 1996a,b<24 h S,M,T,D S 24.13 23.16 PIPR98S 64.08 Erickson et al. 1996a,b<24 h S,M,T,D S 36.83 34.99 PIPR99S 97.49 Erickson et al. 1996a,b<24 h S,M,T,D S 27.94 27.94 PIPR100S 78.99 Erickson et al. 1996a,b<24 h S,M,T,D S 26.67 26.67 PIPR101S 72.86 Erickson et al. 1996a,b<24 h S,M,T,D S 20.32 20.32 PIPR102S 50.73 Erickson et al. 1996a,b<24 h S,M,T,D S 26.67 26.67 PIPR103S 68.24 Erickson et al. 1996a,b<24 h S,M,T,D S 190.50 182.88 PIPR104S 146.6 Erickson et al. 1996a,b<24 h S,M,T,D S 109.86 96.67 PIPR105S 93.76 Erickson et al. 1996a,b<24 h S,M,T,D S 203.20 182.88 PIPR106S 128.86 Erickson et al. 1996a,b<24 h S,M,T,D S 209.55 190.69 PIPR107S 113.0 Erickson et al. 1996a,b<24 h S,M,T,D S 146.05 127.06 PIPR108S 101.01 Erickson et al. 1996a,b<24 h S,M,T,D S 165.10 148.59 PIPR109S 120.9 Erickson et al. 1996a,b<24 h S,M,T,D S 254.00 223.52 PIPR110S 137.6 Erickson et al. 1996a,b<24 h S,M,T,D S 311.15 283.15 PIPR111S 142.9 Erickson et al. 1996a,b<24 h S,M,T,D S 165.10 150.24 PIPR112S 106.74 Erickson et al. 1996a,b<24 h S,M,T,D S 920.75 644.53 PIPR113S 131.9 Erickson et al. 1996a,b<24 h S,M,T,D S 1073.15 697.55 PIPR114S 116.5 Erickson et al. 1996a,b31Table 1. Acute Toxicity of Copper to Freshwater AnimalsSpeciesaOrganism Age,Size, or LifestageMethodbChemicalcReported LC50 orEC50(total μg/L)dReported LC50or EC50(Diss. μg/L)eBLM Data LabelBLM NormalizedLC50 or EC50(μg/L)fSpecies Mean AcuteValue (μg/L)gReference<24 h S,M,T,D S1003.30752.48PIPR115S109.8Erickson et al. 1996a,b<24 h S,M,T,D S933.45653.42PIPR116S123.2Erickson et al. 1996a,b<24 h S,M,T,D S 742.95 646.37 PIPR117S 129.6 Erickson et al. 1996a,b<24 h S,M,T,D S 1879.60 939.80 PIPR118S 124.8 Erickson et al. 1996a,b<24 h S,M,T,D S 266.70 253.37 PIPR119S 176.1 Erickson et al. 1996a,b--- F,M,T S 114.00 --- PIPR120F 17.99 Lind et al. Manuscript (1978)--- F,M,T S 121.00 --- PIPR121F 19.70 Lind et al. Manuscript (1978)--- F,M,T S 88.50 --- PIPR122F 13.27 Lind et al. Manuscript (1978)--- F,M,T S 436.00 --- PIPR123F 78.50 Lind et al. Manuscript (1978)--- F,M,T S 516.00 --- PIPR124F 50.09 Lind et al. Manuscript (1978)--- F,M,T S 1586.00 --- PIPR125F 66.49 Lind et al. Manuscript (1978)--- F,M,T S 1129.00 --- PIPR126F 73.03 Lind et al. Manuscript (1978)--- F,M,T S 550.00 --- PIPR127F 42.76 Lind et al. Manuscript (1978)--- F,M,T S 1001.00 --- PIPR128F 34.39 Lind et al. Manuscript (1978)30 d, 0.15 g F,M,T,D N 96.00 88.32 PIPR129F 39.58 Spehar and Fiandt 1986<24 h F,M,T,D S 31.75 27.94 PIPR130F 8.69 Erickson et al. 1996a,b<24 h F,M,T,D S 117.48 105.73 PIPR131F 37.88 Erickson et al. 1996a,b<24 h F,M,T,D S 48.26 40.06 PIPR132F 10.80 Erickson et al. 1996a,b<24 h F,M,T,D S 73.03 64.26 PIPR133F 22.19 Erickson et al. 1996a,b<24 h F,M,T,D S 59.06 49.02 PIPR134F 20.32 Erickson et al. 1996a,b<24 h F,M,T,D S 78.74 67.72 PIPR135F 18.51 Erickson et al. 1996a,b<24 h F,M,T,D S 22.23 18.67 PIPR136F 13.61 Erickson et al. 1996a,b<24 h F,M,T,D S 6.99 6.15 PIPR137F 10.94 Erickson et al. 1996a,b<24 h F,M,T,D S 22.23 20.45 PIPR138F 17.70 Erickson et al. 1996a,b<24 h F,M,T,D S 107.32 93.36 PIPR139F 67.09 Erickson et al. 1996a,b<24 h F,M,T,D S 292.10 245.36 PIPR140F 17.75 Erickson et al. 1996a,b<24 h F,M,T,D S 81.28 72.34 PIPR141F 41.16 Erickson et al. 1996a,b<24 h F,M,T,D S 298.45 229.81 PIPR142F 16.18 Erickson et al. 1996a,b<24 h F,M,T,D S 241.30 195.45 PIPR143F 24.40 Erickson et al. 1996a,b<24 h F,M,T,D S 133.35 109.35 PIPR144F 21.07 Erickson et al. 1996a,b<24 h F,M,T,D S 93.98 78.00 PIPR145F 50.83 Erickson et al. 1996a,b<24 h F,M,T,D S 67.95 45.52 PIPR146F 23.18 Erickson et al. 1996a,b<24 h F,M,T,D S 4.76 4.38 PIPR147F 40.09 Erickson et al. 1996a,b<24 h F,M,T,D S 13.97 12.43 PIPR148F 45.37 Erickson et al. 1996a,b<24 h F,M,T,D S 29.85 26.86 PIPR149F 59.43 Erickson et al. 1996a,b<24 h F,M,T,D S 59.69 51.33 PIPR150F 58.84 Erickson et al. 1996a,bNorthern squawfish, larval, 0.32 g S,M,T S 380 --- PTLU01S 88.44 132.2 Dwyer et al. 1995Ptychocheilus oregolarval, 0.34 g S,M,T S 480 --- PTLU02S 197.6 Dwyer et al. 199532Table 1. Acute Toxicity of Copper to Freshwater AnimalsSpeciesaOrganism Age,Size, or LifestageMethodbChemicalcReported LC50 orEC50(total μg/L)dReported LC50or EC50(Diss. μg/L)eBLM Data LabelBLM NormalizedLC50 or EC50(μg/L)fSpecies Mean AcuteValue (μg/L)gReferenceNorthern squawfish, 5.0 cm, 1.33 g F,M,T C23---PTOR01F17.0214.61 Andros and Garton 1980Ptychocheilus orego7.2 cm, 3.69 g F,M,T C18---PTOR02F12.54Andros and Garton 1980Razorback sucker, larval, 0.31 g S,M,T S220---XYTE01S63.7878.66 Dwyer et al. 1995Xyrauchen texanuslarval, 0.32 g S,M,T S340---XYTE02S97.0Dwyer et al. 1995Gila topminnow,Poeciliposisid t li2.72 cm, 0.219 g S,M,T S160---POAC01S56.1556.15 Dwyer et al. 1999Bluegill,3.58 cm, 0.63 g R,M,D C-2200LEMA01R22022231 Blaylock et al. 1985Lepomis macrochiru12 cm, 35 g F,M,TS1100---LEMA02F2305Benoit 19752.8-6.8 cm F,M,T C1000---LEMA03F4200Cairns et al. 19813.58 cm, 0.63 g F,M,D C-1300LEMA04F1163Blaylock et al. 1985Fantail darter,3.7 cmS,M,T S330---ETFL01S117.7124.3 Lydy and Wissing 1988Etheostoma flabellar3.7 cmS,M,T S341---ETFL02S121.1Lydy and Wissing 19883.7 cmS,M,T S373---ETFL03S122.8Lydy and Wissing 19883.7 cmS,M,T S392---ETFL04S136.6Lydy and Wissing 1988Greenthroat darter,Etheostoma2.26 cm, 0.133 g S,M,T S260---ETLE01S82.8082.80 Dwyer et al. 1999Johnny darter,3.9 cmS,M,T S493---ETNI01S167.3178.3 Lydy and Wissing 1988Etheostoma nigrum3.9 cmS,M,T S483---ETNI02S164.2Lydy and Wissing 19883.9 cmS,M,T S602---ETNI03S200.1Lydy and Wissing 19883.9 cmS,M,T S548---ETNI04S183.9Lydy and Wissing 1988Fountain darter,Etheostoma rubrum2.02 cm, 0.062 g S,M,T S60---ETRU01S22.7422.74 Dwyer et al. 1999Boreal toad,Bufo boreastadpole, 0.012 g S,M,T S120---BUBO01S47.4947.49 Dwyer et al. 1999aSpecies appear in order taxonomically, with invertebrates listed first, fish, and an amphibian listed last. Species within each genus are ordered alphabetically. Within each species, tests are ordered bytest method (static, renewal, flow-through) and date.bS = static, R = renewal, F = flow-through, U = unmeasured, M = measured, T = exposure concentrations were measured as total copper, D = exposure concentrations were measured asdissolved copper.cS = copper sulfate, N = copper nitrate, C = copper chloride.dValues in this column are total copper LC50 or EC50 values as reported by the author.eValues in this column are dissolved copper LC50 or EC50 values either reported by the author or if the author did not report a dissolved value then a conversion factor (CF) was appliedto the total copper LC50 to estimate dissolved copper values.g Underlined LC50s or EC50s not used to derive SMAV because considered extreme value.33Table 2a. Chronic Toxicity of Copper to Freshwater AnimalsChronicValueb(μg/L)EC20b(μg/L)ACRRotifer,Brachionus calyciflorusLC,T Copper sulfate Intrinsic growthrate85 2.5-5.0 3.54 - 3.54 3.54 Janssen et al. 1994Snail,Campeloma decisum (Test 1)LC,T Copper sulfate Survival 35-55 8-14.8 10.88 8.73 9.77 9.77 191.6 Arthur and Leonard 1970Snail,Campeloma decisum (Test 2)LC,T Copper sulfate Survival 35-55 8-14.8 10.88 10.94 153.0 Arthur and Leonard 1970Cladoceran,Ceriodaphnia dubia (New River)LC,D - Reproduction 179 6.3-9.97.90c(8.23)- 19.3 19.3 3.599 Belanger et al. 1989Cladoceran,Ceriodaphnia dubia (Cinch River)LC,D - Reproduction 94.1 <19.3-19.3 <19.319.36c(20.17)3.271 Belanger et al. 1989Cladoceran,Ceriodaphnia dubiaLC,T Copper sulfate Survival andreproduction57 - 24.50 - 0.547 Oris et al. 1991Cladoceran,Ceriodaphnia dubiaLC,T Copper sulfate Survival andreproduction57 - 34.60 - Oris et al. 1991Cladoceran,Ceriodaphnia dubiaLC,T,D Copper chloride Reproduction 12-32 19.59 9.17 2.069 Carlson et al. 1986Cladoceran,Daphnia magnaLC,T Copper chloride Reproduction 85 10-30 17.32 - 14.1 8.96 Blaylock et al. 1985Cladoceran,Daphnia magnaLC,T Copper chloride Carapace length 225 12.6-36.8 21.50 - van Leeuwen et al. 1988Cladoceran,Daphnia magnaLC,T Copper chloride Reproduction 51 11.4-16.3 13.63 12.58 2.067 Chapman et al. ManuscriptCladoceran,Daphnia magnaLC,T Copper chloride Reproduction 104 20-43 29.33 19.89 1.697 Chapman et al. ManuscriptCladoceran,Daphnia magnaLC,T Copper chloride Reproduction 211 7.2-12.6 9.53 6.06 11.39 Chapman et al. ManuscriptCladoceran,Daphnia pulexLC,T Copper sulfate Survival 57.5 (No HA) 4.0-6.0 4.90 2.83 5.68 9.104 Winner 1985Cladoceran,Daphnia pulexLC,T Copper sulfate Survival 115 (No HA) 5.0-10.0 7.07 3.904 Winner 1985Cladoceran,Daphnia pulexLC,T Copper sulfate Survival 230 (0.15 HA) 10-15 12.25 9.16 3.143 Winner 1985Hardness(mg/L asCaCO3)ChronicLimits (μg/L)SpeciesTestaChemical EndpointGenus MeanChronic Value(Total μg/L)Species MeanChronic Value(Total μg/L)ReferenceChronic Values34Table 2a. Chronic Toxicity of Copper to Freshwater AnimalsChronicValueb(μg/L)EC20b(μg/L)ACRHardness(mg/L asCaCO3)ChronicLimits (μg/L)SpeciesTestaChemical EndpointGenus MeanChronic Value(Total μg/L)Species MeanChronic Value(Total μg/L)ReferenceChronic ValuesCaddisfly,Clistoronia magnificaLC,T Copper chloride Emergence (adult1st gen)26 8.3-13 10.39 7.67 7.67 7.67 Nebeker et al. 1984bRainbow trout,Oncorhynchus mykissELS,TcontinuousCopper chloride Biomass 120 27.77 23.8 11.9 2.881 Seim et al. 1984Rainbow trout,Oncorhynchus mykissELS,T Copper sulfate Biomass 160-180 12-22 16.25 20.32 Besser et al. 2001Chinook salmon,Oncorhynchus tshawytschaELS,T Copper chloride Biomass 20-45 <7.4 <7.4 5.92 5.92 5.594 Chapman 1975, 1982Brown trout,Salmo truttaELS,T Copper sulfate Biomass 45.4 20.8-43.8 29.91 - 29.9 29.9 McKim et al. 1978Brook trout,Salvelinus fontinalisPLC,T Copper sulfate Biomass 35.0 <5 -5 <5 - 12.5 19.7 Sauter et al. 1976Brook trout,Salvelinus fontinalisELS,T Copper sulfate Biomass 45.4 22.3-43.5 31.15 - McKim et al. 1978Lake trout,Salvelinus namaycushELS, T Copper sulfate Biomass 45.4 22.0-43.5 30.94 - 30.9 McKim et al. 1978Northern pike,Esox luciusELS, T Copper sulfate Biomass 45.4 34.9-104.4 60.36 - 60.4 60.4 McKim et al. 1978Bluntnose minnowPimephales notatusLC,T Copper sulfate Egg production 172-230 <18-18 18.00 - 18.0 13.0 12.88 Horning and Neiheisel 1979Fathead minnow,Pimephales promelasELS,T,D - Biomass 45 9.38 9.38 11.40 Lind et al. manuscriptWhite sucker,Catostomus commersoniELS, T Copper sulfate Biomass 45.4 12.9-33.8 20.88 - 20.9 20.9 McKim et al. 1978Bluegill (larval),Lepomis macrochirusELS,T,D Copper sulfate Survival 44-50 21-40 28.98 27.15 27.2 27.2 40.52 Benoit 1975aLC = life-cycle; PLC = partial life-cyle; ELS = early life state; T = total copper; D = dissolved copper.bResults are based on copper, not the chemical.cChronic values based on dissolved copper concentration.35Table 2b. Chronic Toxicity of Copper to Saltwater AnimalsSpeciesTest ChemicalSalinity(g/kg)Limits (μg/L)Chronic Value(μg/L)Chronic Value Dissolved(μg/L)ACR ReferenceSheepshead minnow,Cyprinodon variegatusELS Copper chloride 30 172-362 249 206.7 1.48 Hughes et al. 198936

### Table 2c. Acute-Chronic Ratios

Species Hardness (mg/L

as CaCO 3 ) Acute Value (μg/L) Chronic Value (μg/L) Ratio Reference Overall

Ratio for Species

Snail, 35-55 1673 a 8.73 191.61 Arthur and Leonard 1970 Campeloma decisum 35-55 1673 a 10.94 152.95 Arthur and Leonard 1970 171.19
Cladoceran, 179 28.42 b 7.90 3.60 Belanger et al. 1989 Ceriodaphnia dubia 94.1 63.33 b 19.36 3.27 Belanger et al. 1989

57 13.4 24.5 0.55 Oris et al. 1991

-- 1 7 .974 c 9.17 1.96 C arlson et al. 1986 2

. 85 g 

### > Cladoceran, 51 26 12.58 2.07 Chapman et al. Manuscript Daphnia magna 104 33.76 d 19.89 1.70 Chapman et al. Manuscript

211 69 6.06 11.39 Chapman et al. Manuscript 3.42 

### > Cladoceran, 57.5 25.737 2.83 9.10 Winner 1985

Daphnia pulex 115 27.6 7.07 3.90 Winner 1985

230 28.79 9.16 3.14 Winner 1985 4.82 

### > Rainbow trout, Oncorhynchus mykiss 120 80 27.77 2.88 Seim et al. 1984 2.88 

### > Chinook salmon, Oncorhynchus tshawytscha 20-45 33.1 5.92 5.59 Chapman 1975, 1982 5.59 

### > Bluntnose minnow, Pimephales notatus 172-230 231.9 e 18 12.88 Horning and Neiheisel 1979 12.88
Fathead minnow, Pimephales promelas 45 106.875 f 9.38 11.40 Lind et al. 1978 11.40 Bluegill, Lepomis macrochirus 21-40 1100 27.15 40.52 Benoit 1975 40.49 Sheepshead minnow, Cyprinodon variegatus

- 368 249 1.48 Hughes et al. 1989 1.48 

### >

a Geometric mean of two values from Arthur and Leonard (1970) in Table 1.

b Geometric mean of five values from Belanger et al. (1989) in Table 1. ACR is based on dissolved metal measurements.

c Geometric mean of two values from Carlson et al. (1986) in Table 1.

d Geometric mean of two values from Chapman manuscript in Table 1.

e Geometric mean of two values of three values from Horning and Neiheisel (1979) in Appendix C.

f Geometric mean of three values from Lind et al. (1978) in Table 1.

g ACR from Oris et al. (1991) not used in calculating overall ratio for species because it is <1.



### FACR

Freshwater final acute-chronic ratio = 3.2 2
Saltwater final acute-chronic ratio = 3.2 2

37

### Table 3a. Ranked Freshwater Genus Mean Acute Values with Species Mean

Acute-Chronic Ratios

Rank GMAV Species SMAV (μg/L) ACR



### 27 107,860 Golden shiner , Notemigonus crysoleucas 107,860



### 26 20,636 Stonefly , Acroneuria lycorias 20,636



### 25 3,573 Snail, Campeloma decisum 3,573 171.19



### 24 2,231 Bluegill sunfish , Lepomis macrochirus 2,231 40.49



### 23 1,987 Midge, Chironomus decorus 1,987



### 22 216.3 Chiselmouth , Acrocheilus alutaceus 216.3



### 21 80.38 Fantail darter , Etheostoma flabellare 124.3

Greenthroat darter, Etheostoma lepidum 82.80

Johnny darter , Etheostoma nigrum 178.3

Fountain darter , Etheostoma rubrum 22.74



### 20 78.66 Razorback sucker , Xyrauchen texanus 78.66



### 19 69.63 Fathead minnow, Pimephales promelas 69.63 11.40



### 18 69.63 Shovelnose sturgeon , Scaphirhynchus platorynchus 69.63



### 17 68.31 Bull trout, Salvelinus confluentus 68.31



### 16 63.22 Bonytail chub, Gila elegans 63.22



### 15 56.15 Gila topminnow, Poeciliposis occidentalis 56.15



### 14 52.51 Freshwater mussel, Utterbackia imbecillis 52.51



### 13 48.41 Worm, Lumbriculus variegatus 48.41



### 12 47.49 Boreal toad, Bufo boreas 47.49



### 11 43.94 Colorado squawfish , Ptychocheilus lucius 132.2

Northern squawfish , Ptychocheilus oregonensis 14.61



### 10 31.39 Apache trout , Oncorhynchus apache 32.54

Cutthroat trout , Oncorhynchus clarki 32.97

Pink salmon , Oncorhynchus gorbuscha 40.13

Coho salmon , Oncorhynchus kisutch 22.93

Rainbow trout , Oncorhynchus mykiss 22.19 2.88

Sockeye salmon , Oncorhynchus nerka 54.82

Chinook salmon , Oncorhynchus tshawytscha 25.02 5.59



### 9 20.41 Snail, Physa integra 20.41



### 8 12.31 Snail, Juga plicifera 12.31



### 7 12.07 Amphipod, Hyalella azteca 12.07



### 6 11.33 Freshwater mussel , Actinonaias pectorosa 11.33



### 5 9.73 Cladoceran, Scapholeberis sp. 9.73



### 4 9.60 Amphipod, Gammarus pseudolimnaeus 9.60



### 3 6.67 Snail, Lithoglyphus virens 6.67



### 2 5.93 Cladoceran , Ceriodaphnia dubia 5.93 2.85



### 1 4.05 Cladoceran, Daphnia magna 6.00 3.42

Cladoceran, Daphnia pulicaria 2.73



### 38

### Table 3b. Freshwater Final Acute Value (FAV) and Criteria Calculations

Calculated Freshwater FAV based on 4 lowest values: Total Number of GMAVs in Data Set = 27

Ran k GMAV lnGMAV (lnGMAV) 2 P = R/(n+1) SQRT(P)

4 9.600 2.261 5.114 0.143 0.378

3 6.670 1.897 3.599 0.107 0.327

2 5.930 1.780 3.170 0.071 0.267

1 4.050 1.398 1.954 0.036 0.189

Sum: 7.33671 13.83657 0.35714 1.16153

S = 4.374

L = 0.5641

A = 1.542

Calculated FAV = 4.674452

Calculated CMC = 2.337

Dissolved Copper Criterion Maximum Concentration (CMC) = 2.337 μg/L (for example normalization chemistry see Table 1, footnote f) Criteria Lethal Accumulation (LA50) based on example normalization chemistry = 0.03395 nmol/g wet wt Criterion Continuous Concentration (CCC) = 4.67445/3.22 = 1.4516932 μg/L (for example normalization chemistry see Table 1, footnote f)

S = Scale parameter or slope
L = Location parameter or intercept P = Cumulative probability

A = lnFAV



### 39Table 4. Toxicity of Copper to Freshwater PlantsSpeciesMethodaChemicalHardness (mg/Las CaCO3)Duration EffectResultb(Total μg/L)ReferenceBlue-green alga,Anabaena flos-aquaS,UCoppersulfate65.2 96 hrEC75(cell density)200 Young and Lisk 1972Bllue-green alga,Anabaena variabilisS,U Copper sulfate 65.2 -EC85(wet weight)100 Young and Lisk 1972Blue-green alga,Anabaenastrain 7120- - - - Lag in growth 64 Laube et al. 1980Blue-green alga,Chroococcus parisS,U Copper nitrate 54.7 10 days Growth reduction 100 Les and Walker 1984Blue-green alga,Microcystis aeruginosaS,U Copper sulfate 54.9 8 days Incipient inhibition 30Bringmann 1975; Bringmann and Kuhn1976, 1978a,bAlga,Ankistrodesmus braunii- - - - Growth reduction 640 Laube et al. 1980Green alga,Chlamydomonassp.S,U Copper sulfate 68 10 days Growth inhibition 8,000 Cairns et al. 1978Green alga,Chlamydomonas reinhardtiiS,M,T - 90 - 133 72 hrNOEC(deflagellation)12.2-49.1 Winner and Owen 1991aGreen alga,Chlamydomonas reinhardtiiS,M,T - 90 - 133 72 hrNOEC(cell density)12.2-43.0 Winner and Owen 1991aGreen alga,Chlamydomonas reinhardtiiF,M,T - 24 10 daysEC50(cell density)31.5 Schafer et al. 1993Green alga,Chlorella pyrenoidosaS,U - - 96 hr ca. 12 hr lag in growth 1Steeman-Nielsen and Wium-Andersen1970Green alga,Chlorella pyrenoidosaS,U - 54.7 - Growth inhibition 100Steeman-Nielsen and Kamp-Nielsen1970Green alga,Chlorella pyrenoidosaS,U Copper sulfate 365 14 daysEC50(dry weight)78-100 Bednarz and Warkowska-Dratnal 1985Green alga,Chlorella pyrenoidosaS,U Copper sulfate 36.5 14 daysEC50(dry weight)78-100 Bednarz and Warkowska-Dratnal 1985Green alga,Chlorella pyrenoidosaS,U Copper sulfate 3.65 14 daysEC50(dry weight)78-100Bednarz and Warkowska-Dratnal1983/1984Green alga,Chlorella saccharophilaS,UCopperchloride- 96 hr 96-h EC50 550 Rachlin et al. 1982Green alga,Chlorella vulgarisS,U Copper sulfate 2,000 96 hr Growth inhibition 200 Young and Lisk 1972Green alga,Chlorella vulgarisS,UCopperchloride33 daysEC20(growth)42 Rosko and Rachlin 1977Green alga,Chlorella vulgarisF,U Copper sulfate - 96 hrEC50 or EC50(cell numbers)62 Ferard et al. 1983Green alga,Chlorella vulgarisS,M,D Copper sulfate - 96 hr IC50 270 Ferard et al. 1983Green alga,Chlorella vulgarisS,M,TCopperchloride- 96 hrEC50(cell density)200 Blaylock et al. 1985Green alga,Chlorella vulgarisS,U Copper sulfate 17.1 7 days 15% reduction in cell density 100 Bilgrami and Kumar 199740Table 4. Toxicity of Copper to Freshwater PlantsSpeciesMethodaChemicalHardness (mg/Las CaCO3)Duration EffectResultb(Total μg/L)ReferenceGreen alga,Scenedesmus quadricaudaS,U Copper sulfate 68 10 days Growth reduction 8,000 Cairns et al. 1978Green alga,Scenedesmus quadricaudaS,U Copper sulfate 181 7 daysLOEC(growth)1,100Bringmann and Kuhn 1977a, 1978a,b,1979, 1980aGreen alga,Selenastrum capricornutumS,UCopperchloride14.9 14 daysEC50(cell volume)85 Christensen et al. 1979Green alga,Selenastrum capricornutumS,UCopperchloride14.9 7 daysLOEC(growth)50 Bartlett et al. 1974Green alga,Selenastrum capricornutumS,M,TCopperchloride24.2 96 hrEC50(cell count)400 Blaylock et al. 1985Green alga,Selenastrum capricornutumS,U Copper sulfate 9.3 96 hrEC50(cell count)48.4 Blaise et al. 1986Green alga,Selenastrum capricornutumS,U Copper sulfate 9.3 96 hrEC50(cell count)44.3 Blaise et al. 1986Green alga,Selenastrum capricornutumS,U Copper sulfate 9.3 96 hrEC50(cell count)46.4 Blaise et al. 1986Green alga,Selenastrum capricornutumS,UCopperchloride15 2-3 wkEC50(biomass)53.7 Turbak et al. 1986Green alga,Selenastrum capricornutumS,U Copper sulfate 14.9 5 days Growth reduction 58 Nyholm 1990Green alga,Selenastrum capricornutumS,U Copper sulfate 9.3 96 hrEC50(cell count)69.9 St. Laurent et al. 1992Green alga,Selenastrum capricornutumS,U Copper sulfate 9.3 96 hrEC50(cell count)65.7 St. Laurent et al. 1992Green alga,Selenastrum capricornutumS,U Copper sulfate 24.2 96 hrEC50(cell count)54.4 Radetski et al. 1995Green alga,Selenastrum capricornutumR,U Copper sulfate 24.2 96 hrEC50(cell count)48.2 Radetski et al. 1995Green alga,Selenastrum capricornutumS,U Copper sulfate 16 96 hrEC50(cell density)38 Chen et al. 1997Algae,mixed cultureS,U Copper sulfate - -Significant reduction in blue-greenalgae and nitrogen fixation5 Elder and Horne 1978Diatom,Cyclotella meneghinianaS,U Copper sulfate 68 10 days Growth inhibition 8,000 Cairns et al. 1978Diatom,Navicula incertaS,UCopperchloride- 96 hr EC50 10,429 Rachlin et al. 1983Diatom,Nitzschia linearis- - - 5 day EC50 795-815Academy of Natural Sciences 1960;Patrick et al. 1968Diatom,Nitzschia palea- - - - Complete growth inhibition 5Steeman-Nielsen and Wium-Andersen1970Duckweed,Lemna minorF - - 7 day EC50 119 Walbridge 1977Duckweed,Lemna minorS,U Copper sulfate - 28 days Significant plant damage 130 Brown and Rattigan 197941Table 4. Toxicity of Copper to Freshwater PlantsSpeciesMethodaChemicalHardness (mg/Las CaCO3)Duration EffectResultb(Total μg/L)ReferenceDuckweed,Lemna minorS,U - 0 96 hrEC50(frond number)1,100 Wang 1986Duckweed,Lemna minorS,U Copper sulfate 78 96 hrEC50(chlorophyll a reduction)250 Eloranta et al. 1988Duckweed,Lemna minorR,M,T Copper nitrate 39 96 hr Reduced chlorophyll production 24 Taraldsen and Norberg-King 1990Eurasian watermilfoil,Myriophyllum spicatumS,U - 89 32 daysEC50(root weight)250 Stanley 1974aS=Static; R=Renewal; F=Flow-through; M=Measured; U=Unmeasured; T=Total metal conc. measured; D=dissolved metal conc. measured.bResults are expressed as copper, not as the chemical.42

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# Appendices

# Appendix A. Ranges in Calibration and Application Data Sets010203040

Temperature ( o C)460 Clark Fork River330 Penn. Copper Group30 *Schubauer-Ber., 19910 *Spehar, 198610 *Oris, 199110 *Diamond, 1997b240 *Lind, 1978130 *Lazorchak, 1993100 *Meador, 199140 *Chapman, 198001 *Borgmann, 198304 *Borgmann, 198460 *Baird, 1991017 Dunbar, 1996200 *Winner, 198530 *Schubauer-Ber., 19907 *Collyard, 199440 *Welsh, 1996300 Clark Fork River1050 Erickson, 1996013 Diamond, 1997a013 Dunbar, 199680 *Hagler-Bailly, 199640 *Chapman, 1975, 197801

*Fogels, 197809 *Howarth, 1978110 *Chakoumakos, 197903

*Spear, 197710 *Cusimano, 198602 *Marr, Manuscript01 *Svecevicious, 1996010203040

Temperature ( o C)N___Measured/Nominal/Calculated Data___Assumed/Untraceable DataC. dubia -------------->D.pulicariaD. magna ----------->D. pulexH. aztecaF. minnow ->R. trout ----------------------------->Median, Range and Quartiles of Temperature in BLM Calibration and Application Datasets(All species, Median and Quartiles calculated directly from data i.e., no distributional assumptions)A-10102030405060708090100

HA (%)0 46 Clark Fork River0 33 Penn. Copper Group0 3 *Schubauer-Ber., 1990 1 *Spehar, 19860 1 *Oris, 19910 1 *Diamond, 1997b0 24 *Lind, 19780 13 *Lazorchak, 19930 10 *Meador, 19910 4 *Chapman, 19800 1 *Borgmann, 19830 4 *Borgmann, 19840 6 *Baird, 19910 17 Dunbar, 19960 20 *Winner, 19850 3 *Schubauer-Ber., 1990 7 *Collyard, 19940 4 *Welsh, 19960 30 Clark Fork River0105 Erickson, 19960 13 Diamond, 1997a0 13 Dunbar, 19960 8 *Hagler-Bailly, 19960 4 *Chapman, 1975, 19780 1

*Fogels, 19780 9 *Howarth, 19780 11 *Chakoumakos, 19790 3

*Spear, 19770 1 *Cusimano, 19860 2 *Marr, Manuscript0 1 *Svecevicious, 19960102030405060708090100

HA (%)N___Measured/Nominal/Calculated Data___Assumed/Untraceable DataC. dubia --------------> D.pulicariaD. magna -----------> D. pulex H. azteca F. minnow -> R. trout ----------------------------->Median, Range and Quartiles of HA in BLM Calibration and Application Datasets(All species, Median and Quartiles calculated directly from data i.e., no distributional assumptions)A-245678910

pH46 0 Clark Fork River33 0 Penn. Copper Group3 0 *Schubauer-Ber., 1991 0 *Spehar, 19861 0 *Oris, 19911 0 *Diamond, 1997b17 7 *Lind, 197813 0 *Lazorchak, 199310 0 *Meador, 19914 0 *Chapman, 19800 1 *Borgmann, 19830 4 *Borgmann, 19846 0 *Baird, 19910 17 Dunbar, 199618 2 *Winner, 19853 0 *Schubauer-Ber., 1990 7 *Collyard, 19944 0 *Welsh, 199630 0 Clark Fork River1050 Erickson, 199613 0 Diamond, 1997a0 13 Dunbar, 19968 0 *Hagler-Bailly, 19964 0 *Chapman, 1975, 19780 1

*Fogels, 19780 9 *Howarth, 197811 0 *Chakoumakos, 19790 3

*Spear, 19771 0 *Cusimano, 19860 2 *Marr, Manuscript0 1 *Svecevicious, 199645678910

pHN___Measured/Nominal/Calculated Data___Assumed/Untraceable DataC. dubia --------------> D.pulicariaD. magna -----------> D. pulex H. azteca F. minnow -> R. trout ----------------------------->Median, Range and Quartiles of pH in BLM Calibration and Application Datasets(All species, Median and Quartiles calculated directly from data i.e., no distributional assumptions)A-310-210-1100101102 DOC (mg/L)2026 Clark Fork River2013 Penn. Copper Group0 3 *Schubauer-Ber., 1990 1 *Spehar, 19860 1 *Oris, 19910 1 *Diamond, 1997b17 7 *Lind, 19780 13 *Lazorchak, 199310 0 *Meador, 19914 0 *Chapman, 19800 1 *Borgmann, 19830 4 *Borgmann, 19846 0 *Baird, 19910 17 Dunbar, 199620 0 *Winner, 19850 3 *Schubauer-Ber., 1990 7 *Collyard, 19944 0 *Welsh, 19961119 Clark Fork River0105 Erickson, 19960 13 Diamond, 1997a0 13 Dunbar, 19968 0 *Hagler-Bailly, 19964 0 *Chapman, 1975, 19780 1

*Fogels, 19780 9 *Howarth, 197811 0 *Chakoumakos, 19790 3

*Spear, 19771 0 *Cusimano, 19860 2 *Marr, Manuscript0 1 *Svecevicious, 199610-210-1100101102 DOC (mg/L)N___Measured/Nominal/Calculated Data___Assumed/Untraceable DataC. dubia --------------> D.pulicariaD. magna -----------> D. pulex H. azteca F. minnow -> R. trout ----------------------------->Median, Range and Quartiles of DOC in BLM Calibration and Application Datasets(All species, Median and Quartiles calculated directly from data i.e., no distributional assumptions)A-410-1100101102103 Ca (mg/L as CaCO 3 )46 0 Clark Fork River33 0 Penn. Copper Group3 0 *Schubauer-Ber., 1991 0 *Spehar, 19861 0 *Oris, 19911 0 *Diamond, 1997b17 7 *Lind, 197813 0 *Lazorchak, 199310 0 *Meador, 19914 0 *Chapman, 19800 1 *Borgmann, 19830 4 *Borgmann, 19846 0 *Baird, 19910 17 Dunbar, 199620 0 *Winner, 19853 0 *Schubauer-Ber., 1990 7 *Collyard, 19944 0 *Welsh, 199630 0 Clark Fork River1050 Erickson, 199613 0 Diamond, 1997a0 13 Dunbar, 19968 0 *Hagler-Bailly, 19964 0 *Chapman, 1975, 19780 1

*Fogels, 19780 9 *Howarth, 197811 0 *Chakoumakos, 19790 3

*Spear, 19771 0 *Cusimano, 19860 2 *Marr, Manuscript0 1 *Svecevicious, 199610-1100101102103 Ca (mg/L as CaCO 3 )N___Measured/Nominal/Calculated Data___Assumed/Untraceable DataC. dubia -------------->D.pulicariaD. magna ----------->D. pulexH. aztecaF. minnow ->R. trout ----------------------------->Median, Range and Quartiles of Ca in BLM Calibration and Application Datasets(All species, Median and Quartiles calculated directly from data i.e., no distributional assumptions)A10-210-1100101102103 Mg (mg/L as CaCO 3 )46 0 Clark Fork River33 0 Penn. Copper Group3 0 *Schubauer-Ber., 1991 0 *Spehar, 19861 0 *Oris, 19911 0 *Diamond, 1997b17 7 *Lind, 197813 0 *Lazorchak, 199310 0 *Meador, 19914 0 *Chapman, 19800 1 *Borgmann, 19830 4 *Borgmann, 19846 0 *Baird, 19910 17 Dunbar, 199620 0 *Winner, 19853 0 *Schubauer-Ber., 1990 7 *Collyard, 19944 0 *Welsh, 199630 0 Clark Fork River1050 Erickson, 199613 0 Diamond, 1997a0 13 Dunbar, 19968 0 *Hagler-Bailly, 19964 0 *Chapman, 1975, 19780 1

*Fogels, 19780 9 *Howarth, 197811 0 *Chakoumakos, 19790 3

*Spear, 19771 0 *Cusimano, 19860 2 *Marr, Manuscript0 1 *Svecevicious, 19960102030405060708090100

HA (%)N___Measured/Nominal/Calculated Data___Assumed/Untraceable DataC. dubia --------------> D.pulicariaD. magna -----------> D. pulex H. azteca F. minnow -> R. trout ----------------------------->Median, Range and Quartiles of HA in BLM Calibration and Application Datasets(All species, Median and Quartiles calculated directly from data i.e., no distributional assumptions)A-610-1100101102103 Na (mg/L as Na)46 0 Clark Fork River1320 Penn. Copper Group3 0 *Schubauer-Ber., 1991 0 *Spehar, 19861 0 *Oris, 19911 0 *Diamond, 1997b17 7 *Lind, 197813 0 *Lazorchak, 199310 0 *Meador, 19914 0 *Chapman, 19800 1 *Borgmann, 19830 4 *Borgmann, 19846 0 *Baird, 19910 17 Dunbar, 199620 0 *Winner, 19853 0 *Schubauer-Ber., 1990 7 *Collyard, 19944 0 *Welsh, 199630 0 Clark Fork River1050 Erickson, 19960 13 Diamond, 1997a0 13 Dunbar, 19968 0 *Hagler-Bailly, 19964 0 *Chapman, 1975, 19780 1

*Fogels, 19780 9 *Howarth, 197811 0 *Chakoumakos, 19790 3

*Spear, 19771 0 *Cusimano, 19860 2 *Marr, Manuscript0 1 *Svecevicious, 199610-1100101102103 Na (mg/L as Na)N___Measured/Nominal/Calculated Data___Assumed/Untraceable DataC. dubia --------------> D.pulicariaD. magna -----------> D. pulex H. azteca F. minnow -> R. trout ----------------------------->Median, Range and Quartiles of Na in BLM Calibration and Application Datasets(All species, Median and Quartiles calculated directly from data i.e., no distributional assumptions)A-710-210-1100101102103 K (mg/L as K)46 0 Clark Fork River1320 Penn. Copper Group3 0 *Schubauer-Ber., 1991 0 *Spehar, 19861 0 *Oris, 19911 0 *Diamond, 1997b17 7 *Lind, 197813 0 *Lazorchak, 199310 0 *Meador, 19914 0 *Chapman, 19800 1 *Borgmann, 19830 4 *Borgmann, 19846 0 *Baird, 19910 17 Dunbar, 199620 0 *Winner, 19853 0 *Schubauer-Ber., 1990 7 *Collyard, 19944 0 *Welsh, 199630 0 Clark Fork River1050 Erickson, 19960 13 Diamond, 1997a0 13 Dunbar, 19968 0 *Hagler-Bailly, 19964 0 *Chapman, 1975, 19780 1

*Fogels, 19780 9 *Howarth, 197811 0 *Chakoumakos, 19790 3

*Spear, 19771 0 *Cusimano, 19860 2 *Marr, Manuscript0 1 *Svecevicious, 199610-210-1100101102103 K (mg/L as K)N___Measured/Nominal/Calculated Data___Assumed/Untraceable DataC. dubia --------------> D.pulicariaD. magna -----------> D. pulex H. azteca F. minnow -> R. trout ----------------------------->Median, Range and Quartiles of K in BLM Calibration and Application Datasets(All species, Median and Quartiles calculated directly from data i.e., no distributional assumptions)A-810-210-1100101102103

SO 4 (mg/L as SO 4 )46 0 Clark Fork River1320 Penn. Copper Group3 0 *Schubauer-Ber., 1991 0 *Spehar, 19861 0 *Oris, 19911 0 *Diamond, 1997b17 7 *Lind, 197813 0 *Lazorchak, 199310 0 *Meador, 19914 0 *Chapman, 19800 1 *Borgmann, 19830 4 *Borgmann, 19846 0 *Baird, 19910 17 Dunbar, 19968 12 *Winner, 19853 0 *Schubauer-Ber., 1990 7 *Collyard, 19944 0 *Welsh, 199630 0 Clark Fork River1050 Erickson, 19960 13 Diamond, 1997a0 13 Dunbar, 19968 0 *Hagler-Bailly, 19964 0 *Chapman, 1975, 19780 1

*Fogels, 19780 9 *Howarth, 197811 0 *Chakoumakos, 19790 3

*Spear, 19771 0 *Cusimano, 19860 2 *Marr, Manuscript0 1 *Svecevicious, 199610-210-1100101102103

SO 4 (mg/L as SO 4 )N___Measured/Nominal/Calculated Data___Assumed/Untraceable DataC. dubia --------------> D.pulicariaD. magna -----------> D. pulex H. azteca F. minnow -> R. trout ----------------------------->Median, Range and Quartiles of SO4 in BLM Calibration and Application Datasets(All species, Median and Quartiles calculated directly from data i.e., no distributional assumptions)A-910-1100101102103 Cl (mg/L as Cl)3115 Clark Fork River1320 Penn. Copper Group3 0 *Schubauer-Ber., 1991 0 *Spehar, 19861 0 *Oris, 19911 0 *Diamond, 1997b17 7 *Lind, 197813 0 *Lazorchak, 199310 0 *Meador, 19914 0 *Chapman, 19800 1 *Borgmann, 19830 4 *Borgmann, 19846 0 *Baird, 19910 17 Dunbar, 199620 0 *Winner, 19853 0 *Schubauer-Ber., 1990 7 *Collyard, 19944 0 *Welsh, 19961515 Clark Fork River1050 Erickson, 19960 13 Diamond, 1997a0 13 Dunbar, 19968 0 *Hagler-Bailly, 19964 0 *Chapman, 1975, 19780 1

*Fogels, 19780 9 *Howarth, 197811 0 *Chakoumakos, 19790 3

*Spear, 19771 0 *Cusimano, 19860 2 *Marr, Manuscript0 1 *Svecevicious, 199610-1100101102103 Cl (mg/L as Cl)N___Measured/Nominal/Calculated Data___Assumed/Untraceable DataC. dubia --------------> D.pulicariaD. magna -----------> D. pulex H. azteca F. minnow -> R. trout ----------------------------->Median, Range and Quartiles of Cl in BLM Calibration and Application Datasets(All species, Median and Quartiles calculated directly from data i.e., no distributional assumptions)A-10100101102103 Alkalinity (mg/L as CaCO 3 )460 Clark Fork River330 Penn. Copper Group30 *Schubauer-Ber., 19910 *Spehar, 198610 *Oris, 199110 *Diamond, 1997b177 *Lind, 1978130 *Lazorchak, 1993100 *Meador, 199140 *Chapman, 198001 *Borgmann, 198304 *Borgmann, 198460 *Baird, 1991017 Dunbar, 1996200 *Winner, 198530 *Schubauer-Ber., 19907 *Collyard, 199440 *Welsh, 1996300 Clark Fork River1050 Erickson, 1996130 Diamond, 1997a013 Dunbar, 199680 *Hagler-Bailly, 199640 *Chapman, 1975, 197801

*Fogels, 197809 *Howarth, 1978110 *Chakoumakos, 197903

*Spear, 197710 *Cusimano, 198602 *Marr, Manuscript01 *Svecevicious, 1996100101102103 Alkalinity (mg/L as CaCO 3 )N___Measured/Nominal/Calculated Data___Assumed/Untraceable DataC. dubia -------------->D.pulicariaD. magna ----------->D. pulexH. aztecaF. minnow ->R. trout ----------------------------->Median, Range and Quartiles of Alkalinity in BLM Calibration and Application Datasets(All species, Median and Quartiles calculated directly from data i.e., no distributional assumptions)A-11

# Appendix B. Other Data on Effects of Copper on Freshwater OrganismsSpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceBacteria,Escherichia coliS,U Coppersulfate- 48 hr Threshold of inhibited glucose use;measured by pH change in media80 - Bringmann and Kuhn 1959aBacteria,Pseudomonas putidaS,U Coppersulfate81.1 16 hr EC3(cell numbers)30 - Bringmann and Kuhn 1976, 1977a,1979, 1980aProtozoan,Entosiphon sulcatumS,U Coppersulfate81.9 72 hr EC5(cell numbers)110 - Bringmann 1978;Bringmann and Kuhn 1979, 1980a,Protozoan,Microrega heterostomaS,U Coppersulfate214 28 hr Threshold of decreased feeding rate 50 - Bringmann and Kuhn 1959bProtozoan,Chilomonas parameciumS,U Coppersulfate- 48 hr Growth threshold 3,200 - Bringmann and Kuhn 1980b, 1981Protozoan,Uronema pardueziS,U Coppersulfate- 20 hr Growth threshold 140 - Bringmann and Kuhn 1980b, 1981Protozoa,mixed species- - - 7 days Reduced rate of colonization 167 - Cairns et al. 1980Protozoa,mixed speciesS,M,T Coppersulfate- 15 days Reduced rate of colonization 100 - Buikema et al. 1983Green alga,Cladophora glomerataDosedstreamCoppersulfate226-310 10 mo Decreased abundance from 21% downto 0%120- Weber and McFarland 1981Green alga,Chlamydomonas reinhardtii- Coppersulfate76 72 hr Deflagellation 6.7 - Garvey et al. 1991Green alga,Chlamydomonas reinhardtii- Coppersulfate76 72 hr Deflagellation 6.7 - Garvey et al. 1991Green alga,Chlamydomonas reinhardtii- Coppersulfate76 72 hr Deflagellation 16.3 - Garvey et al. 1991Green alga,Chlamydomonas reinhardti- Coppersulfate7672 hrDeflagellation25.4- Garvey et al. 1991Green alga,Chlorellasp.S,UCoppernitrate-28 hrInhibited photosynthesis6.3- Gachter et al. 1973Green alga,Chlorella pyrenoidosaS,U - 29.4 72 hr IC50(cell division rate)16 - Stauber and Florence 1989Green alga,Chlorella pyrenoidosaS,U - 14.9 72 hr IC50(cell division rate)24 - Stauber and Florence 1989Green alga,Chlorella pyrenoidosaS,U Coppersulfate82 4 hr Disturbedphotosystem II25 - Vavilin et al. 1995Green alga,Eudorina californicaS,U Coppersulfate19.1 - Decrease in cell density 5,000 - Young and Lisk 1972Green alga (flagellate cells),Haematococcussp.S,U Coppersulfate2 24 hr Inhibited growth during 96 hr recoveryperiod50 - Pearlmutter and Buchheim 1983Green alga,Scenedesmus quadricaudaS,U Coppersulfate214 96 hr Threshold of effect on cell numbers 150 - Bringmann and Kuhn 1959bGreen alga,Scenedesmus quadricaudaS,U Coppersulfate60 72 hr EC3(cell numbers)1,100 - Bringmann and Kuhn 1980aGreen alga,Scenedesmus quadricaudaS,U Coppersulfate34.8 24 hr EC50(photosynthesis)100 - Starodub et al. 1987B-1SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceGreen alga,Scenedesmus quadricaudaS,U Coppersulfate34.8 24 hr NOEC(growth)50 - Starodub et al. 1987Green alga,Scenedesmus quadricaudaS,U Coppersulfate34.8 24 hr NOEC(growth)50 - Starodub et al. 1987Green alga,Scenedesmus quadricaudaS,U Coppersulfate34.8 24 hr NOEC(growth)>200 - Starodub et al. 1987Green alga,Selenastrum capricornutumS,U Copperchloride14.9 7 days Growth reduction 50 - Bartlett et al.1974Green alga,Selenastrum capricornutumS,U Coppersulfate29.3 72 hr EC50(cell count)19 - Vasseur et al. 1988Green alga,Selenastrum capricornutumS,U Coppersulfate24.2 72 hr EC50(cell count)41 - Vasseur et al. 1988Green alga,Selenastrum capricornutumS,U Coppersulfate24.2 72 hr EC50(cell count)28 - Vasseur et al. 1988Green alga,Selenastrum capricornutumS,U Coppersulfate14.9 72 hr EC50(cell count)60 - Vasseur et al. 1988Green alga,Selenastrum capricornutumS,U Coppersulfate24.2 72 hr EC50(cell count)28.5 - Benhra et al. 1997Green alga,Selenastrum capricornutumF,U Coppersulfate15 24 hr EC50(cell density)21 - Chen et al. 1997Diatom,Cocconeis placentulaDosedstreamCoppersulfate226-310 10 mo Decreased abundance from 21% downto <1%120 - Weber and McFarland 1981Phytoplankton,mixed speciesS,U - - 124 hr Averaged 39% reduction in primaryproduction10 - Cote 1983Macrophyte,Elodea canadensisS,U Coppersulfate- 24 hr EC50(photosynthesis)150 - Brown and Rattigan 1979Microcosm F,M,T,D Coppersulfate200 32 wk LOEC(primary production)9.3 - Hedtke 1984Microcosm F,M,T,D Coppersulfate200 32 wk NOEC(primary production)4 - Hedtke 1984Microcosm F,M,T Coppersulfate76.7 96 hr Significant drop in no. of taxa and no.of individuals15 - Clements et al. 1988Microcosm F,M,T Coppersulfate58.5 10 days Significant drop in no. of individuals 2.5 - Clements et al. 1989Microcosm F,M,T Coppersulfate151 10 days 58% drop in no. of individuals 13.5 - Clements et al. 1989Microcosm F,M,T Coppersulfate68 10 days Significant drop in species richnessand no. of individuals11.3 - Clements et al. 1990Microcosm F,M,T Coppersulfate80 10 days Significant drop in species richnessand no. of individuals10.7 - Clements et al. 1990Microcosm S,M,T Coppersulfate102 5 wk 14-28% drop in phytoplankton speciesrichness20 - Winner and Owen 1991bMicrocosm F,M,T - 160 28 days LOEC(species richness)19.9 - Pratt and Rosenberger 1993B-2SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceDosed streamF,M,D Coppersulfate56 1 yr Shifts in periphyton speciesabundance5.208 - Leland and Carter 1984Dosed stream F,M,D Coppersulfate56 1 yr Reduced algal production 5.208 - Leland and Carter 1985Sponge,Ephydatia fluviatilisS,U Coppersulfate200 10 days Reduced growth by 33% 6 - Francis and Harrison 1988Sponge,Ephydatia fluviatilisS,U Coppersulfate200 10 days Reduced growth by 100% 19 - Francis and Harrison 1988Rotifer,Philodina acuticornisS,U Coppersulfate45 48 hr LC50(50C)1,300 - Cairns et al. 1978Rotifer,Philodina acuticornisS,U Coppersulfate45 48 hr LC50(100C)1,200 - Cairns et al. 1978Rotifer,Philodina acuticornisS,U Coppersulfate45 48 hr LC50(150C)1,130 - Cairns et al. 1978Rotifer,Philodina acuticornisS,U Coppersulfate45 48 hr LC50(200C)1,000 - Cairns et al. 1978Rotifer,Philodina acuticornisS,U Coppersulfate45 48 hr LC50(250C)950 - Cairns et al. 1978Rotifer,Brachionus calyciflorusS, U Coppersulfate39.8 24 hr EC50(mobility)200 - Couillard et al. 1989Rotifer (2 hr),Brachionus calyciflorusS,U Coppersulfate- 2 hr LOEC(swimming activity)12.5 - Charoy et al. 1995Rotifer,Brachionus calyciflorusS,U Coppersulfate90 24 hr EC50(mobility)76 - Ferrando et al. 1992Rotifer (2 hr),Brachionus calyciflorusS,U Coppersulfate90 5 hr EC50(filtration rate)34 - Ferrando et al. 1993aRotifer (2 hr),Brachionus calyciflorusS,U Coppersulfate90 6 days LOEC(reproduction decreased 26%)5 - Janssen et al. 1993Rotifer (2 hr),Brachionus calyciflorusS,U Coppersulfate90 5 hr LOEC(reduced swimming speed)12 - Janssen et al. 1993Rotifer (2 hr),Brachionus calyciflorusS,U Coppersulfate85 3 days LOEC(reproduction decreased 27%)5 - Janssen et al. 1994Rotifer (2 hr),Brachionus calyciflorusS,U Coppersulfate85 3 days LOEC(reproduction decreased 29%)5 - Janssen et al. 1994Rotifer (2 hr),Brachionus calyciflorusS,U Coppersulfate85 8 days LOEC(reproduction decreased 47%)5 - Janssen et al. 1994Rotifer (2 hr),Brachionus calyciflorusS,U Copperchloride170 35 min LOEC(food ingestion rate)100 - Juchelka and Snell 1994Rotifer (2 hr),Brachionus calyciflorusS,U Coppersulfate63.2 24 hr EC50(mobility)9.4 - Porta and Ronco 1993Rotifer (2 hr),Brachionus calyciflorusS,U - 90 2 days LOEC(reproduction decreased 100%)30 - Snell and Moffat 1992Rotifer (<2 hr),Brachionus calyciflorusS, U - 85 24 hr EC50(mobility)26 - Snell et al. 1991bB-3SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceRotifer (<2 hr),Brachionus calyciflorusS, U - 85 24 hr EC50(mobility; 100C)18 - Snell 1991;Snell et al. 1991bRotifer (<2 hr),Brachionus calyciflorusS, U - 85 24 hr EC50(mobility; 150C)31 - Snell 1991;Snell et al. 1991bRotifer (<2 hr),Brachionus calyciflorusS, U - 85 24 hr EC50(mobility; 200C)31 - Snell 1991;Snell et al. 1991bRotifer (<2 hr),Brachionus calyciflorusS, U - 85 24 hr EC50(mobility; 250C)26 - Snell 1991;Snell et al. 1991bRotifer (<2 hr),Brachionus calyciflorusS, U - 85 24 hr EC50(mobility; 300C)25 - Snell 1991;Snell et al. 1991bRotifer (<3 hr),Brachionus rubensS, U Coppersulfate90 24 hr LC50 19 - Snell and Persoone 1989bRotifer,Keratella cochlearisS,U Copperchloride-24 hrLC50101- Borgman and Ralph 1984Worm,Aeolosoma headleyiS,U Coppersulfate45 48 hrLC50(50C)2,600 - Cairns et al. 1978Worm,Aeolosoma headleyiS,U Coppersulfate45 48 hr LC50(100C)2,300 - Cairns et al. 1978Worm,Aeolosoma headleyiS,U Coppersulfate45 48 hr LC50(150C)2,000 - Cairns et al. 1978Worm,Aeolosoma headleyiS,U Coppersulfate45 48 hr LC50(200C)1,650 - Cairns et al. 1978Worm,Aeolosoma headleyiS,U Coppersulfate45 48 hr LC50(50 C)1,000 - Cairns et al. 1978Worm (adult),Lumbriculus variegatusS,U Coppersulfate30 LC50 150 Bailey and Liu, 1980Worm (7 mg),Lumbriculus variegatisF,M,T Coppersulfate45 10 days LC50 35 - West et al. 1993Tubificid worm,Limnodrilus hoffmeisteriS,U Coppersulfate100 LC50 102 Wurtz and Bridges 1961Tubificid worm,Tubifex tubifexR, U Coppersulfate245 LC50 158 Khangarot 1991Snail (11-27 mm),Campeloma decisumF,M,T Coppersulfate45 6 wk LOEC(mortality)14.8 - Arthur and Leonard 1970Snail,Gyraulus circumstriatusS,U Coppersulfate100 LC50 108 Wurtz and Bridges 1961Snail,Goniobasis livescensS,U Coppersulfate154 48 hr LC50 860 - Cairns et al. 1976Snail,Goniobasis livescensS,M,D Coppersulfate154 96 hr LC50 - 390 Paulson et al. 1983Snail,Nitrocrissp.S,U Coppersulfate45 48 hr LC50(50C)3,000 - Cairns et al. 1978Snail,Nitrocrissp.S,U Coppersulfate45 48 hr LC50(100C)2,400 - Cairns et al. 1978B-4SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceSnail,Nitrocrissp.S,U Coppersulfate45 48 hr LC50(150C)1,000 - Cairns et al. 1978Snail,Nitrocrissp.S,U Coppersulfate45 48 hr LC50(200C)300 - Cairns et al. 1978Snail,Nitrocrissp.S,U Coppersulfate45 48 hr LC50(250C)210 - Cairns et al. 1978Snail,Lymnaea emarginataS,U Coppersulfate154 48 hr LC50 300 - Cairns et al. 1976Snail (adult),Juga pliciferaF,M,T Copperchloride23 30 days LC50 6 - Nebeker et al. 1986bSnail (adult),Lithoglyphus virensF,M,T Copperchloride23 30 days LC50 4 - Nebeker et al. 1986bSnail,Physa heterostrophaS,U Coppersulfate100 LC50 69 Wurtz and Bridges 1961Freshwater mussel (releasedglochidia),Actinonaias pectorosaR,M Coppersulfate140 24 hr 132 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Actinonaias pectorosaR,M Coppersulfate150 24 hr 93 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Actinonaias pectorosaR,M Coppersulfate170 24 hr 67 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Actinonaias pectorosaR,M Coppersulfate140 24 hr 42 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Actinonaias pectorosaR,M Coppersulfate170 48 hr 51 Jacobson et al. 1997Freshwater mussel (1-2 d),Anodonta grandisS,M,T Coppersulfate70 24 hr LC50 44 - Jacobson et al. 1993Freshwater mussel (1-2 d),Anodonta imbecilisS,M,T Coppersulfate39 48 hr LC50 171 - Keller and Zam 1991Freshwater mussel (1-2 d),Anodonta imbecilisS,M,T Coppersulfate90 48 hr LC50 388 - Keller and Zam 1991Freshwater mussel (releasedglochidia),LampsilisfasciolaR,M,T Coppersulfate170 24 hr 48 Jacobson et al. 1997Freshwater mussel (releasedglochidia),LampsilisfasciolaR,M,T Coppersulfate160 24 hr 26 Jacobson et al. 1997Freshwater mussel (releasedglochidia),LampsilisfasciolaR,M,T Coppersulfate75 24 hr 46 Jacobson et al. 1997B-5SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceFreshwater mussel (releasedglochidia),LampsilisfasciolaR,M,T Coppersulfate170 48 hr 40 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Medionidus conradicusR,M,T Coppersulfate185 24 hr 69 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Medionidus conradicusR,M,T Coppersulfate185 24 hr 40 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Medionidus conradicusR,M,T Coppersulfate185 24 hr 37 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Medionidus conradicusR,M,T Coppersulfate170 24 hr 46 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Medionidus conradicusR,M,T Coppersulfate160 24 hr 41 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Medionidus conradicusR,M,T Coppersulfate150 24 hr 81 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Medionidus conradicusR,M,T Coppersulfate170 48 hr 16 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Pygranodon grandisR,M,T Coppersulfate170 24 hr >160 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Pygranodon grandisR,M,T Coppersulfate170 24 hr 347 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Pygranodon grandisR,M,T Coppersulfate50 24 hr 46 Jacobson et al. 1997Freshwater mussel (1-2 d),Villosa irisS,M,T Coppersulfate190 24 hr LC50 83 - Jacobson et al. 1993Freshwater mussel (releasedglochidia),Villosa irisR,M,T Coppersulfate190 24 hr 80 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Villosa irisR,M,T Coppersulfate190 24 hr 73 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Villosa irisR,M,T Coppersulfate185 24 hr 65 Jacobson et al. 1997B-6SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceFreshwater mussel (releasedglochidia),Villosa irisR,M,T Coppersulfate185 24 hr 46 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Villosa irisR,M,T Coppersulfate170 24 hr 75 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Villosa irisR,M,T Coppersulfate160 24 hr 46 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Villosa irisR,M,T Coppersulfate160 24 hr 36 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Villosa irisR,M,T Coppersulfate155 24 hr 39 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Villosa irisR,M,T Coppersulfate155 24 hr 37 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Villosa irisR,M,T Coppersulfate150 24 hr 46 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Villosa irisR,M,T Coppersulfate150 24 hr 46 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Villosa irisR,M,T Coppersulfate55 24 hr 55 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Villosa irisR,M,T Coppersulfate55 24 hr 38 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Villosa irisR,M,T Coppersulfate50 24 hr 71 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Villosa irisR,M,T Coppersulfate160 24 hr 46 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Villosa irisR,M,T Coppersulfate170 48 hr 66 Jacobson et al. 1997Freshwater mussel (releasedglochidia),Villosa irisR,M,T Coppersulfate150 48 hr 46 Jacobson et al. 1997Zebra mussel (1.6-2.0 cm),Dreissena polymorphaR,M,T Copperchloride268 9 wk EC50+F106(filtration rate)43 - Kraak et al. 1992B-7SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceZebra mussel (1.6-2.0 cm),Dreissena polymorphaR,M,T Copperchloride268 10 wk NOEC(filtration rate)13 - Kraak et al. 1993Asiatic clam (1.0-2.1 cm),Coprbicula flumineaS,M,T Coppersulfate64 96 hr (24hrLC50 alsoreported)LC50 40 - Rodgers et al. 1980Asiatic clam (1.0-2.1 cm),Coprbicula flumineaF,M,T Coppersulfate64 96 hr (24 hrLC50 alsoreported)LC50 490 - Rodgers et al. 1980Asiatic clam (juvenile),Corbicula flumineaF,M,D Coppersulfate78 30 days 43.3% mortality 14.48 - Belanger et al. 1990Asiatic clam (juvenile),Corbicula flumineaF,M,D Coppersulfate78 30 days Stopped shell growth 8.75 - Belanger et al. 1990Asiatic clam (adult),Corbicula flumineaF,M,D Coppersulfate78 30 days 13.3% mortality 14.48 - Belanger et al. 1990Asiatic clam (adult),Corbicula flumineaF,M,D Coppersulfate71 30 days 25% mortality 16.88 - Belanger et al. 1990Asiatic clam (adult),Corbicula flumineaF,M,D Coppersulfate78 30 days Inhibited shell growth 8.75 - Belanger et al. 1990Asiatic clam (adult),Corbicula flumineaF,M,D Coppersulfate- 15-16 days LC50 - - Belanger et al. 1991Asiatic clam (adult),Corbicula flumineaF,M,D Coppersulfate- 19 days LC100 - - Belanger et al. 1991Asiatic clam (veliger larva),Corbicula manilensisS,M,T Copperchloride- 24 hr 34% mortality 10 - Harrison et al. 1981, 1984Asiatic clam (juvenile),Corbicula manilensisS,M,T Copperchloride17 24 hr LC50 100 - Harrison et al. 1984Asiatic clam (veliger),Corbicula manilensisS,M,T Copperchloride17 24 hr LC50 28 - Harrison et al. 1984Asiatic clam (trochophore),Corbicula manilensisS,M,T Copperchloride17 8 hr LC100 7.7 - Harrison et al. 1984Asiatic clam (adult),Corbicula manilensisF,M,T Copperchloride17 7 days LC50 3,638 - Harrison et al. 1981, 1984Asiatic clam (adult),Corbicula manilensisF,M,T Copperchloride17 42 days LC50 12 - Harrison et al. 1981, 1984Asiatic clam (4.3 g adult),Corbicula manilensisF,M,T Copperchloride17 30 days LC50 11 - Harrison et al. 1984Cladoceran,Bosmina longirostrusS, U Coppersulfate33.8 EC50 1.6 Koivisto et al. 1992Cladoceran (<24 hr),Daphnia ambiguaS,U Coppersulfate145 72 hr LC50 86.5 - Winner and Farrell 1976Cladoceran (<24 hr),Daphnia ambiguaS,U Coppersulfate145 Life span(ca. 5 wk)Chronic limits (inst. rate of populationgrowth)50 - Winner and Farrell 1976Cladoceran,Ceriodaphnia dubiaS,U Coppersulfate188 EC50 36.6 Bright 1995B-8SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceCladoceran,Ceriodaphnia dubiaS,U Coppersulfate204 EC50 19.1 Bright 1995Cladoceran,Ceriodaphnia dubiaS,U Coppersulfate428 EC50 36.4 Bright 1995Cladoceran,Ceriodaphnia dubiaS,U Coppersulfate410 EC50 11.7 Bright 1995Cladoceran,Ceriodaphnia dubiaS,U Coppersulfate494 EC50 12.3 Bright 1995Cladoceran,Ceriodaphnia dubiaS,U Coppersulfate440 EC50 12 Bright 1995Cladoceran,Ceriodaphnia dubiaS,U Copperchloride90 1 hr NOEC(ingestion)30 - Juchelka and Snell 1994Cladoceran (<24 hr),Ceriodaphnia dubiaS,M,D Coppersulfate6-10 48 hr LC50 - 2.72 Suedel et al. 1996Cladoceran (<12 hr),Ceriodaphnia dubiaS,M,D - 113.6 48 hr LC50 - 52 Belanger and Cherry 1990Cladoceran (<12 hr),Ceriodaphnia dubiaS,M,D - 113.6 48 hr LC50 - 76 Belanger and Cherry 1990Cladoceran (<12 hr),Ceriodaphnia dubiaS,M,D - 113.6 48 hr LC50 - 91 Belanger and Cherry 1990Cladoceran (<48 h),Ceriodaphnia dubiaS,M,T Coppernitrate280 - 300 48 hr LC50 9.5 - Schubauer-Berigan et al. 1993Cladoceran (<48 h),Ceriodaphnia dubiaS,M,T Coppernitrate280 - 300 48 hr LC50 28 - Schubauer-Berigan et al. 1993Cladoceran (<48 h),Ceriodaphnia dubiaS,M,T Coppernitrate280 - 300 48 hr LC50 200 - Schubauer-Berigan et al. 1993Cladoceran (<24 hr),Ceriodaphnia dubiaS,M,T,D Coppernitrate100 48 hr LC50 66 60.72 Spehar and Fiandt 1986Cladoceran,Ceriodaphnia dubiaR,U Coppernitrate111 10 days LC50 53 - Cowgill and Milazzo 1991aCladoceran,Ceriodaphnia dubiaR,U Coppernitrate111 10 days NOEC(reproduction)96 - Cowgill and Milazzo 1991aCladoceran,Ceriodaphnia dubiaR,U Coppersulfate90 - LOEC(reproduction)44 - Zuiderveen and Birge 1997Cladoceran,Ceriodaphnia dubiaR,U Coppersulfate90 - LOEC(reproduction)40 - Zuiderveen and Birge 1997Cladoceran,Ceriodaphnia dubiaR,M,T - 20 - IC50(reproduction)5 - Jop et al. 1995Cladoceran (<24 hrs),Ceriodaphnia reticulataS, U Copperchloride240 EC50 23 Elnabarawy et al. 1986Cladoceran,Ceriodubia reticulataS,U - 43-45 EC50 17 Mount and Norberg 1984Cladoceran,Daphnia magna- Coppersulfate- 72 hr EC50(mobility; 100C)61 - Braginskij and Shcherben 1978B-9ciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceCladoceran,Daphnia magna- Coppersulfate- 72 hr EC50(mobility; 150C)70 - Braginskij and Shcherben 1978Cladoceran,Daphnia magna- Coppersulfate- 72 hr EC50(mobility; 200C)21 - Braginskij and Shcherben 1978Cladoceran,Daphnia magna- Coppersulfate- 72 hr EC50(mobility; 300C)9.3 - Braginskij and Shcherben 1978Cladoceran,Daphnia magnaS,U Coppersulfate- 16 hr EC 50(mobility)38 - Anderson 1944Cladoceran (<8 hr),Daphnia magnaS,U Copperchloride- 64 hr Immobilization threshold 12.7 - Anderson 1948Cladoceran (1 mm),Daphnia magnaS,U Coppernitrate100 24 hr EC 50(mobility)50 - Bellavere and Gorbi 1981Cladoceran (1 mm),Daphnia magnaS,U Coppernitrate200 24 hr EC 50(mobility)70 - Bellavere and Gorbi 1981Cladoceran,Daphnia magnaS,U - 100 48 hr EC50(mobility)254 - Borgmann and Ralph 1983Cladoceran,Daphnia magnaS,U - 100 49 hr EC50(mobility)1,239 - Borgmann and Ralph 1983Cladoceran,Daphnia magnaS,U Coppersulfate45 48 hr EC50(mobility; 50C)90 - Cairns et al. 1978Cladoceran,Daphnia magnaS,U Coppersulfate45 48 hr EC50(mobility; 100C)70 - Cairns et al. 1978Cladoceran,Daphnia magnaS,U Coppersulfate45 48 hr EC50(mobility; 150C)40 - Cairns et al. 1978Cladoceran,Daphnia magnaS,U Coppersulfate45 48 hr EC50(mobility; 250C)7 - Cairns et al. 1978Cladoceran (4 days),Daphnia magnaS,U Coppersulfate- 24 hr EC50(filtration rate)59 - Ferrando and Andreu 1993Cladoceran (24-48 hr),Daphnia magnaS,U Coppersulfate90 24 hr EC50(mobility)380 - Ferrando et al. 1992Cladoceran,Daphnia magnaS,U Coppersulfate50 EC50 7 Oikari et al. 1992Cladoceran,Daphnia magnaS,U Coppersulfate- 48 hr EC50(mobility)45 - Oikari et al. 1992Cladoceran (<24 hr),Daphnia magnaS,U Coppersulfate145 Life span(ca. 18 wk)Chronic limits(inst. rate of population growth)70 - Winner and Farrell 1976Cladoceran (<24 hrs),Daphnia magnaS,M,D Coppersulfate72-80 48 hr LC50 - 11.3 Suedel et al. 1996Cladoceran (<24 hrs),Daphnia magnaS,M,I - 180 - LC50 55.3 - Borgmann and Charlton 1984Cladoceran (<24 hr),Daphnia magnaS,M,I Coppersulfate100 48 hr EC50(mobility)46.0 - Meador 1991Cladoceran (<24 hr),Daphnia magnaS,M,I Coppersulfate100 48 hr EC50(mobility)57.2 - Meador 1991B-10SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceCladoceran (<24 hr),Daphnia magnaS,M,I Coppersulfate100 48 hr EC50(mobility)67.8 - Meador 1991Cladoceran (<24 hr),Daphnia magnaS,M,T Coppersulfate100 72 hr EC50(mobility)52.8 - Winner 1984bCladoceran (<24 hr),Daphnia magnaS,M,T Coppersulfate100 72 hr EC50(mobility)56.3 - Winner 1984bCladoceran (<24 hr),Daphnia magnaS,M,T Copperchloride85 96 hr EC50(mobility)130 - Blaylock et al. 1985Cladoceran (24 hr),Daphnia magnaR,U Coppersulfate- 48 hr EC50(mobility)18 - Kazlauskiene et al. 1994Cladoceran (<24 hr),Daphnia parvulaS,U Coppersulfate145 72 hr EC50(mobility)72 - Winner and Farrell 1976Cladoceran (<24 hr),Daphnia parvulaS,U Coppersulfate145 72 hr EC50(mobility)57 - Winner and Farrell 1976Cladoceran (<24 hr),Daphnia parvulaS,U Coppersulfate145 Life span(ca. 10 wk)Chronic limits (inst. rate of populationgrowth)50 - Winner and Farrell 1976Cladoceran,Daphnia pulexS,U Coppersulfate45 EC50 10 Cairns et al. 1978Cladoceran,Daphnia pulexS,U - 45 EC50 53 Mount and Norberg 1984Cladoceran (<24 hrs),Daphnia pulexS, U Copperchloride240 EC50 31 Elnabarawy et al. 1986Cladoceran (<24 hrs),Daphnia pulexS, U Coppersulfate33.8 EC50 3.6 Koivisto et al. 1992Cladoceran (<24 hrs),Daphnia pulexS,U Copperchloride80-90 EC50 18 Roux et al. 1993Cladoceran (<24 hrs),Daphnia pulexS,U Copperchloride80-90 EC50 24 Roux et al. 1993Cladoceran (<24 hrs),Daphnia pulexS,U Copperchloride80-90 EC50 22 Roux et al. 1993Cladoceran (<24 hr),Daphnia pulexS,U Coppersulfate145 72 hr EC50(mobility)86 - Winner and Farrell 1976Cladoceran (<24 hr),Daphnia pulexS,U Coppersulfate145 72 hr EC50(mobility)54 - Winner and Farrell 1976Cladoceran (<24 hr),Daphnia pulexS,U Coppersulfate145 Life span(ca. 7 wk)Chronic limits (inst. rate of populationgrowth)50 - Winner and Farrell 1976Cladoceran,Daphnia pulexS,U Coppersulfate45 48 hr EC50(mobility)70 - Cairns et al. 1978Cladoceran,Daphnia pulexS,U Coppersulfate45 48 hr EC50(mobility)60 - Cairns et al. 1978Cladoceran,Daphnia pulexS,U Coppersulfate45 48 hr EC50(mobility)20 - Cairns et al. 1978Cladoceran,Daphnia pulexS,U Coppersulfate45 48 hr EC50(mobility)56 - Cairns et al. 1978B-11SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceCladoceran (<24 hr),Daphnia pulexS,U Coppersulfate200 24 hr EC50(mobility)37.5 - Lilius et al. 1995Cladoceran,Daphnia pulexS,M,T Coppersulfate106 48 hr EC50(mobility)29 - Ingersoll and Winner 1982Cladoceran,Daphnia pulexS,M,T Coppersulfate106 48 hr EC50(mobility)20 - Ingersoll and Winner 1982Cladoceran,Daphnia pulexS,M,T Coppersulfate106 48 hr EC50(mobility)25 - Ingersoll and Winner 1982Cladoceran,Daphnia pulexR,U Coppersulfate85 21 days Reduced fecundity 3 - Roux et al. 1993Cladoceran,Daphnia pulexR,M,T Coppersulfate106 70 days Significantly shortened life span;reduced brood size20 - Ingersoll and Winner 1982Cladoceran,Daphnia pulicariaS,M,T - 31 48 hr EC50(mobility; TOC=14 mg/L)55.4 - Lind et al. manuscriptCladoceran,Daphnia pulicariaS,M,T - 29 49 hr EC50(mobility; TOC=13 mg/L)55.3 - Lind et al. manuscriptCladoceran,Daphnia pulicariaS,M,T - 28 50 hr EC50(mobility; TOC=13 mg/L)53.3 - Lind et al. manuscriptCladoceran,Daphnia pulicariaS,M,T - 28 50 hr EC50(mobility; TOC=28 mg/L)97.2 - Lind et al. manuscriptCladoceran,Daphnia pulicariaS,M,T - 100 51 hr EC50(mobility; TOC=34 mg/L)199 - Lind et al. manuscriptCladoceran,Daphnia pulicariaS,M,T - 86 52 hr EC50(mobility; TOC=34 mg/L)627 - Lind et al. manuscriptCladoceran,Daphnia pulicariaS,M,T - 84 53 hr EC50(mobility; TOC=32 mg/L)165 - Lind et al. manuscriptCladoceran,Daphnia pulicariaS,M,T - 16 54 hr EC50(mobility; TOC=12 mg/L)35.5 - Lind et al. manuscriptCladoceran,Daphnia pulicariaS,M,T - 151 55 hr EC50(mobility; TOC=13 mg/L)78.8 - Lind et al. manuscriptCladoceran,Daphnia pulicariaS,M,T - 96 56 hr EC50(mobility; TOC=28 mg/L)113 - Lind et al. manuscriptCladoceran,Daphnia pulicariaS,M,T - 26 57 hr EC50(mobility; TOC=25 mg/L)76.4 - Lind et al. manuscriptCladoceran,Daphnia pulicariaS,M,T - 84 58 hr EC50(mobility; TOC=13 mg/L)84.7 - Lind et al. manuscriptCladoceran,Daphnia pulicariaS,M,T - 92 59 hr EC50(mobility; TOC=21 mg/L)184 - Lind et al. manuscriptCladoceran,Daphnia pulicariaS,M,T - 106 60 hr EC50(mobility; TOC=34 mg/L)240 - Lind et al. manuscriptCladoceran,Daphnia pulicariaS,M,T Coppersulfate106 48 hr LC50 240 - Lind et al. manuscriptCladoceran,Simocephalus serrulatusS,M,T Coppernitrate8 24 hr EC50(mobility; TOC=11 mg/L)12 - Giesy et al. 1983B-12SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceCladoceran,Simocephalus serrulatusS,M,T Coppernitrate16 25 hr EC50(mobility; TOC=12.4 mg/L)7.2 - Giesy et al. 1983Cladoceran,Simocephalus serrulatusS,M,T Coppernitrate16 26 hr EC50(mobility; TOC=15.6 mg/L)24.5 - Giesy et al. 1983Cladoceran (<24 hr),Simocephalus vetulusS,U - 45 57 Mount and Norberg 1984Cladoceran (life cycle),Bosmina longirostrisR,U Coppersulfate-13 daysLOEC(intrinsic rate of population increase)18- Koivisto and Ketola 1995Copepods (mixed sp),PrimarilyAcanthocyclopsvernalisandDiacyclops thomasiR,M,I Copperchloride-1 wkEC20(growth)42- Borgmann and Ralph 1984Copepod (adults and copepodidsV),Tropocyclops prasinusmexicanusS, U Coppersulfate1029Lalande and Pinel-Alloul 1986Copepod (adults and copepodidsV),TropocyclopsprasinusmexicanusS, U Coppersulfate10 96 hr LC50 247 - Lalande and Pinel-Alloul 1986Amphipod (0.4 cm),Crangonyx pseudogracilisR,U Coppersulfate45-55 1290 Martin and Holdich 1986Amphipod (4 mm),Crangonyx psuedogracilisR,U Coppersulfate50 48 hr LC50 2,440 - Martin and Holdich 1986Amphipod,Gammarus fasciatusS,U Coppersulfate206 48 hr LC50 210 - Judy 1979Amphipod,Gammarus lacustrisS,U Coppersulfate- 96 hr LC50 1,500 - Nebeker and Gaufin 1964Amphipod (2-3 wk),Hyallela aztecaS,M,T Coppersulfate6-10 - LC50 65.6 - Suedel et al. 1996Amphipod (0-1 wk),Hyallela aztecaR,M,T Coppernitrate130 10 wk Significant mortality 25.4 - Borgmann et al. 1993Amphipod (7-14 days),Hyallela aztecaF,M,T Coppersulfate46 10 days LC50 31 - West et al. 1993Crayfish (intermoult adult,19.6 g),Cambarus robustusS,M,D - 10-12 96 hr LC50 - 830 Taylor et al. 1995Crayfish (1.9-3.2 cm),Orconectes limosusS,M,T Copperchloride- 96 hr LC50 600 - Boutet and Chaisemartin 1973Crayfish (3.0-3.5 cm),Orconectes rusticusF,U Coppersulfate100-125 3,000 Hubschman 1967Crayfish (embryo),Orconectes rusticusF,U Coppersulfate113 2 wk 52% mortality of newlyhatched young250 - Hubschman 1967B-13SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceCrayfish (3.14 mg dry wt.),Orconectes rusticusF,U Coppersulfate113 2 wk 23% reduction in growth 15 - Hubschman 1967Crayfish (30-40 mm),Orconectessp.- 113 48 hr LC50 2,370 - Dobbs et al. 1994Crayfish,Procambarus clarkiiF,M,T Copperchloride17 1358 hr LC50 657 - Rice and Harrison 1983Mayfly (6th-8th instar),Stenonemasp.S,M,T - 110 48 hr LC50 453 - Dobbs et al. 1994Mayfly,Cloeon dipterium- Coppersulfate- 72 hr LC50(100C)193 - Braginskij and Shcherban 1978Mayfly,Cloeon dipterium- - - 72 hr LC50(150C)95.2 - Braginskij and Shcherban 1978Mayfly,Cloeon dipterium- - - 72 hr LC50(250C)53 - Braginskij and Shcherban 1978Mayfly,Cloeon dipterium- - - 72 hr LC50(300C)4.8 - Braginskij and Shcherban 1978Mayfly,Ephemerella grandisF,M,T Coppersulfate50 14 days LC50 180-200 - Nehring 1976Mayfly,Ephemerella subvariaS,M Coppersulfate44 48 hr LC50 320 - Warnick and Bell 1969Mayfly (6th-8th instar),Isonychia bicolorS,M,T - 110 48 hr LC50 223 - Dobbs et al. 1994Stonefly,Pteronarcys californicaF,M,T Coppersulfate50 14 days LC50 12,000 - Nehring 1976Caddisfly,Hydropsyche betteniS,M,T Coppersulfate44 14 days LC50 32,000 - Warnick and Bell 1969Midge (2nd instar),Chironomus ripariusS,M,T - 110 48 hr LC50 1,170 - Dobbs et al. 1994Midge (1st instar),Chironomus tentansS,U Coppersulfate42.7 16.7 Gauss et al. 1985Midge (1st instar),Chironomus tentansS,U Coppersulfate109.6 36.5 Gauss et al. 1985Midge (1st instar),Chironomus tentansS,U Coppersulfate172.3 98.2 Gauss et al. 1985Midge (4th instar),Chironomus tentansS,U Coppersulfate42.7 211 Gauss et al. 1985Midge (4th instar),Chironomus tentansS,U Coppersulfate109.6 977 Gauss et al. 1985Midge (4th instar),Chironomus tentansS,U Coppersulfate172.3 1184 Gauss et al. 1985Midge,Chironomus tentansS,U Coppersulfate25 327 Khangarot and Ray 1989Midge (2nd instar),Chironomus tentansS,M,T Coppersulfate8 96 hr LC50 630 - Suedel et al. 1996B-14SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceMidge (4th instar),Chironomus tentansF,M,T Copperchloride36 20 days LC50 77.5 - Nebeker et al. 1984bMidge (embryo),Tanytarsus dissimilisS,M,T Copperchloride46.8 10 days LC50 16.3 - Anderson et al. 1980Midge,UnidentifiedF,M,T,D Coppersulfate200 32 wk Emergence 30 - Hedtke 1984Bryozoan (2-3 day ancestrula),Lophopodella carteriS,U - 190-220 510 Pardue and Wood 1980Bryozoan (2-3 day ancestrula),Pectinatella magnificaS,U - 190-220 140 Pardue and Wood 1980Bryozoan (2-3 day ancestrula),Plumatella emarginataS,U - 190-220 140 Pardue and Wood 1980American eel (5.5 cm glass eelstage),Anguilla rostrataS,U Coppersulfate40-48 96 hr LC50 2,540 Hinton and Eversole 1978American eel (9.7 cm black eelstage),Anguilla rostrataS,U Coppersulfate40-48 96 hr LC50 3,200 Hinton and Eversole 1979American eel,Anguilla rostrataS,M,T Coppernitrate53 96 hr LC50 6,400 - Rehwoldt et al. 1971American eel,Anguilla rostrataS,M,T Coppernitrate55 96 hr LC50 6,000 - Rehwoldt et al. 1972Arctic grayling (larva),Thymallus arcticusS,U Coppersulfate41.3 96 hr LC50 67.5 Buhl and Hamilton 1990Arctic grayling (larva),Thymallus arcticusS,U Coppersulfate41.3 96 hr LC50 23.9 Buhl and Hamilton 1990Arctic grayling (larva),Thymallus arcticusS,U Coppersulfate41.3 96 hr LC50 131 Buhl and Hamilton 1990Arctic grayling (swim-up),Thymallus arcticusS,U Coppersulfate41.3 96 hr LC50 9.6 Buhl and Hamilton 1990Arctic grayling (0.20 g juvenile),Thymallus arcticusS,U Coppersulfate41.3 96 hr LC50 2.7 Buhl and Hamilton 1990Arctic grayling (0.34 g juvenile),Thymallus arcticusS,U Coppersulfate41.3 96 hr LC50 2.58 Buhl and Hamilton 1990Arctic grayling (0.81 g juvenile),Thymallus arcticusS,U Coppersulfate41.3 96 hr LC50 49.3 Buhl and Hamilton 1990Arctic grayling (0.85 g juvenile),Thymallus arcticusS,U Coppersulfate41.3 96 hr LC50 30 Buhl and Hamilton 1990Coho salmon (larva),Oncorhynchus kisutchS,U Coppersulfate41.3 96 hr LC50 21 Buhl and Hamilton 1990Coho salmon (larva),Oncorhynchus kisutchS,U Coppersulfate41.3 96 hr LC50 19.3 Buhl and Hamilton 1990Coho salmon (0.41 g juvenile),Oncorhynchus kisutchS,U Coppersulfate41.3 96 hr LC50 15.1 Buhl and Hamilton 1990B-15SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceCoho salmon (0.47 g juvenile),Oncorhynchus kisutchS,U Coppersulfate41.3 96 hr LC50 23.9 Buhl and Hamilton 1990Coho salmon (0.87 g juvenile),Oncorhynchus kisutchS,U Coppersulfate41.3 96 hr LC50 31.9 Buhl and Hamilton 1990Coho salmon (10 cm),Oncorhynchus kisutchS,U Coppersulfate- 72 hr LC50 280 - Holland et al. 1960Coho salmon (9.7 cm),Oncorhynchus kisutchS,U Coppersulfate- 72 hr LC50 190 - Holland et al. 1960Coho salmon (9.7 cm),Oncorhynchus kisutchS,U Coppersulfate- 72 hr LC50 480 - Holland et al. 1960Coho salmon (juvenile),Oncorhynchus kisutchR,M,T,I - 33 96 hr LC50(TOC=7.3 mg/L)164 - Buckley 1983Coho salmon (juvenile),Oncorhynchus kisutchR,M,T,I - 33 96 hr LC50 286 Buckley 1983Coho salmon (6.3 cm),Oncorhynchus kisutchF,U Coppersulfate- 30 days LC50 360 - Holland et al. 1960Coho salmon (6.3 cm),Oncorhynchus kisutchF,U Coppersulfate- 72 hr LC50 370 - Holland et al. 1960Coho salmon (smolts),Oncorhynchus kisutchF,M,T Copperchloride91 144 hr Decrease in survival upon transfer to30 ppt seawater20 - Lorz and McPherson 1976Coho salmon (smolts >10 cm),Oncorhynchus kisutchF,M,T Copperchloride91 165 days Decrease in downstream migrationafter release5 - Lorz and McPherson 1976Coho salmon (7.8 cm),Oncorhynchus kisutchF,M,T Copperacetate276 14 wk 15% reduction in growth 70 - Buckley et al. 1982Coho salmon (7.8 cm),Oncorhynchus kisutch- - 276 7 days LC50 220 - Buckley et al. 1982Coho salmon (3-8 g),Oncorhynchus kisutchF,M,T Copperacetate280 7 days LC50 275 - McCarter and Roch 1983Coho salmon (3-8 g),Oncorhynchus kisutchF,M,T Copperacetate280 7 days LC50 (acclimated to copper for 2 wk) 383 - McCarter and Roch 1983Coho salmon (parr),Oncorhynchus kisutchF,M,T,D,I - 24.4 61 days NOEC(growth and survival)22 - Mudge et al. 1993Coho salmon,Oncorhynchus kisutchF,M,T,D,I - 31.1 60 days NOEC(growth and survival)18 - Mudge et al. 1993Coho salmon (parr),Oncorhynchus kisutchF,M,T,D,I - 31 61 days NOEC(growth and survival)33 - Mudge et al. 1993Rainbow trout (15-40g)Oncorhynchus mykissF,M, Copperchloride-- 120 hr LA50 (50% mortality) ~1.4 ug Cu/g gill - MacRae et al. 1999Sockeye salmon (yeasrling),Oncorhynchus nerkaS,U Coppersulfate12 1-24 hr Drastic increase in plasmacorticosteroids64 - Donaldson and Dye 1975Sockeye salmon (fry, 0.132 g,2.95 cm),Oncorhynchus nerkaR,M,T Copperchloride36-46 96 hr LC50 220 - Davis and Shand 1978B-16SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceSockeye salmon (fry, 0.132 g,2.95 cm),Oncorhynchus nerkaR,M,T Copperchloride36-46 96 hr LC50 210 - Davis and Shand 1978Sockeye salmon (fry, 0.132 g,2.95 cm),Oncorhynchus nerkaR,M,T Copperchloride36-46 96 hr LC50 240 - Davis and Shand 1978Sockeye salmon (fry, 0.132 g,2.95 cm),Oncorhynchus nerkaR,M,T Copperchloride36-46 96 hr LC50 103 - Davis and Shand 1978Sockeye salmon (fry, 0.132 g,2.95 cm),Oncorhynchus nerkaR,M,T Copperchloride36-46 96 hr LC50 240 - Davis and Shand 1978Chinook salmon (18-21 weeks),Oncorhynchus tshawytschaS,U Coppersulfate211 96 hr LC50 58 Hamilton and Buhl 1990Chinook salmon (18-21 weeks),Oncorhynchus tshawytschaS,U Coppersulfate211 96 hr LC50 54 Hamilton and Buhl 1990Chinook salmon (18-21 weeks),Oncorhynchus tshawytschaS,U Coppersulfate343 96 hr LC50 60 Hamilton and Buhl 1990Chinook salmon (5.2 cm),Oncorhynchus tshawytschaS,U Coppernitrate- 5 days LC50 178 - Holland et al. 1960Chinook salmon (eyed embryos),Oncorhynchus tshawytschaF,M,D Coppersulfate44 26 days 93% mortality 41.67 - Hazel and Meith 1970Chinook salmon (alevin),Oncorhynchus tshawytschaF,M,T Copperchloride23 200 hr LC50 20 - Chapman 1978Chinook salmon (alevin),Oncorhynchus tshawytschaF,M,T Copperchloride23 200 hr LC10 15 - Chapman 1978Chinook salmon (swimup),Oncorhynchus tshawytschaF,M,T Copperchloride23 200 hr LC50 19 - Chapman 1978Chinook salmon (swimup),Oncorhynchus tshawytschaF,M,T Copperchloride23 200 hr LC10 14 - Chapman 1978Chinook salmon (parr),Oncorhynchus tshawytschaF,M,T Copperchloride23 200 hr LC50 30 - Chapman 1978Chinook salmon (parr),Oncorhynchus tshawytschaF,M,T Copperchloride23 200 hr LC10 17 - Chapman 1978Chinook salmon (smolt),Oncorhynchus tshawytschaF,M,T Copperchloride23 200 hr LC50 26 - Chapman 1978Chinook salmon (smolt),Oncorhynchus tshawytschaF,M,T Copperchloride23 200 hr LC10 18 - Chapman 1978Chinook salmon (3.9-6.8 cm),Oncorhynchus tshawytschaF,M,T Coppersulfate20-22 96 hr LC50 32 - Finlayson and Verrue 1982Cutthroat trout (3-5 mo),Oncorhynchus clarkiF,M Copperchloride50 20 min avoidance of copper 7.708 - Woodward et al. 1997B-17SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceRainbow trout,Oncorhynchus mykiss- - 320 48 hr LC50 500 - Brown 1968Rainbow trout (9-16 cm),Oncorhynchus mykissIn situ - 21-26 48 hr LC50 70 - Calamari and Marchetti 1975Rainbow trout (0.4 g),Oncorhynchus mykissS,U Coppersulfate- 96 hr LC50 185 - Bills et al. 1981Rainbow trout (larva),Oncorhynchus mykissS, U Coppersulfate41.3 96 hr LC50 36 Buhl and Hamilton 1990Rainbow trout (0.60 g juvenile),Oncorhynchus mykissS, U Coppersulfate41.3 96 hr LC50 13.8 Buhl and Hamilton 1990Rainbow trout (13-15 cm),Oncorhynchus mykissS,U Coppersulfate250 72 hr LC50 580 - Brown et al. 1974Rainbow trout (13-15 cm),Oncorhynchus mykissS,U Coppersulfate250 72 hr LC50 960 - Brown et al. 1974Rainbow trout (3.2 cm),Oncorhynchus mykissS,U Coppersulfate- 24 hr LC50 140 - Shaw and Brown 1974Rainbow trout (3.2 cm),Oncorhynchus mykissS,U Coppersulfate- 24 hr LC50 130 - Shaw and Brown 1974Rainbow trout (4.0-10.6 cm),Oncorhynchus mykissS,U Coppersulfate45 24 hr LC50(50C)950 - Cairns et al. 1978Rainbow trout (4.0-10.6 cm),Oncorhynchus mykissS,U Coppersulfate45 24 hr LC50(150C)430 - Cairns et al. 1978Rainbow trout (4.0-10.6 cm),Oncorhynchus mykissS,U Coppersulfate45 24 hr LC50(300C)150 - Cairns et al. 1978Rainbow trout (0.52-1.55 g),Oncorhynchus mykissS,U Coppersulfate- 96 hr LC50(Silver Cup diet)23.9 - Marking et al. 1984Rainbow trout (0.41-2.03 g),Oncorhynchus mykissS,U Coppersulfate- 96 hr LC50(purified H440)11.3 - Marking et al. 1984Rainbow trout (0.0.40-1.68 g),Oncorhynchus mykissS,U Coppersulfate- 96 hr LC50(SD-9 diet)15.9 - Marking et al. 1984Rainbow trout (0.0.34-1.52 g),Oncorhynchus mykissS,U Coppersulfate- 96 hr LC50(liver diet)14.3 - Marking et al. 1984Rainbow trout (0.0.38-1.30 g),Oncorhynchus mykissS,U Coppersulfate- 96 hr LC50(brine shrimp diet)11.3 - Marking et al. 1984Rainbow trout (embryo),Oncorhynchus mykissS,U Copperchloride30 56 hr LC50 100 - Rombough 1985Rainbow trout (6.6 cm),Oncorhynchus mykissR,U Coppersulfate320 72 hr LC50 1,100 - Lloyd 1961Rainbow trout (6.6 cm),Oncorhynchus mykissR,U Coppersulfate17.5 7 days LC50 44 - Lloyd 1961Rainbow trout,Oncorhynchus mykissR,U Coppersulfate320 48 hr LC50 270 - Herbert and Vandyke 1964Rainbow trout (yearling),Oncorhynchus mykissR,U Coppersulfate240 48 hr LC50 750 - Brown and Dalton 1970B-18SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceRainbow trout (13-15 cm),Oncorhynchus mykissR,U Coppersulfate250 8 days LC50 500 - Brown et al. 1974Rainbow trout (embryo),Oncorhynchus mykissR,U Coppersulfate104 28 days LC50 90 - Birge 1978;Birge et al. 1978Rainbow trout (embryo),Oncorhynchus mykissR,U Coppersulfate101 28 days EC50(death or deformity)110 - Birge et al. 1980;Birge and Black 1979Rainbow trout (embryo),Oncorhynchus mykissR,U Coppersulfate101 28 days EC10(death or deformity)16.5 - Birge et al. 1980Rainbow trout (eyed embryos),Oncorhynchus mykissR,U Coppersulfate- 96 hr LC50 1,150 - Kazlauskiene et al. 1994Rainbow trout (larva),Oncorhynchus mykissR,U Coppersulfate- 96 hr LC50 430 - Kazlauskiene et al. 1994Rainbow trout (16-18 cm),Oncorhynchus mykissR,U Coppersulfate- 96 hr LC50 930 - Kazlauskiene et al. 1994Rainbow trout (embryo),Oncorhynchus mykissR,M,T Coppersulfate62.9 7-9 mo Lesions in olfactory rosettes 22 - Saucier et al. 1991bRainbow trout (embryo),Oncorhynchus mykissR,M,T Coppersulfate62.9 7-9 mo 31% mortality 22 - Saucier et al. 1991bRainbow trout (eyed embryos),Oncorhynchus mykissR,M,T Coppersulfate40-48 96 hr LC50 400 - Giles and Klaverkamp 1982Rainbow trout (yearling),Oncorhynchus mykissR,M,T Coppersulfate36.5 21 days Elevated plasma cortisol returnedto normal45 - Munoz et al. 1991Rainbow trout (embryo),Oncorhynchus mykissR,M,T Coppersulfate44 96 hr 15-20% post-hatch mortality 80 - Giles and Klaverkamp 1982Rainbow trout (embryo),Oncorhynchus mykissR,M,T Coppersulfate62.9 7-9 mo Inhibited olfactory discrimination 22 - Saucier et al. 1991aRainbow trout (5.1-7.6 cm),Oncorhynchus mykissF,U Coppernitrate- 96 hr LC50 253 - Hale 1977Rainbow trout (11 cm),Oncorhynchus mykissF,U - 100 96 hr LC50 250 - Goettl et al. 1972Rainbow trout (5 wk postswimup)Oncorhynchus mykissF,U Coppersulfate89.5 1 hr Avoidance 10 - Folmar 1976Rainbow trout (18.5-26.5 cm),Oncorhynchus mykissF,U Coppersulfate90 2 hr 55% depressed olfactory response 50 - Hara et al. 1976Rainbow trout (3.2 cm),Oncorhynchus mykissF,M,I Coppersulfate- 8 days LC50 500 - Shaw and Brown 1974Rainbow trout (12-16 cm),Oncorhynchus mykissF,M,T Coppersulfate300 14 days LC50 870 - Calamari and Marchetti 1973Rainbow trout (adult),Oncorhynchus mykissF,M,T Copperchloride42 - LC50 57 - Chapman 1975, Chapman andStevens 1978Rainbow trout (53.5 g),Oncorhynchus mykissF,M,T Coppersulfate365 96 hr LC50 465 - Lett et al. 1976B-19SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceRainbow trout (53.5 g),Oncorhynchus mykissF,M,T Coppersulfate365 15 days Transient decrease in foodconsumption100 - Lett et al. 1976Rainbow trout (alevin),Oncorhynchus mykissF,M,T Copperchloride24 200 hr LC50 20 - Chapman 1978Rainbow trout (alevin),Oncorhynchus mykissF,M,T Copperchloride24 200 hr LC10 19 - Chapman 1978Rainbow trout (swimup),Oncorhynchus mykissF,M,T Copperchloride24 200 hr LC50 17 - Chapman 1978Rainbow trout (swimup),Oncorhynchus mykissF,M,T Copperchloride24 200 hr LC10 9 - Chapman 1978Rainbow trout (parr),Oncorhynchus mykissF,M,T Copperchloride25 200 hr LC50 15 - Chapman 1978Rainbow trout (parr),Oncorhynchus mykissF,M,T Copperchloride25 200 hr LC10 8 - Chapman 1978Rainbow trout (smolt),Oncorhynchus mykissF,M,T Copperchloride25 200 hr LC50 21 - Chapman 1978Rainbow trout (smolt),Oncorhynchus mykissF,M,T Copperchloride25 200 hr LC10 7 - Chapman 1978Rainbow trout,Oncorhynchus mykissF,M,T Coppersulfate112.4 80 min Avoidance threshold 74 - Black and Birge 1980Rainbow trout (>8 g),Oncorhynchus mykissF,M,T Coppersulfate49 15-18 days LC50 48 - Miller and MacKay 1980Rainbow trout (>8 g),Oncorhynchus mykissF,M,T Coppersulfate51 15-18 days LC50 46 - Miller and MacKay 1980Rainbow trout (>8 g),Oncorhynchus mykissF,M,T Coppersulfate57 15-18 days LC50 63 - Miller and MacKay 1980Rainbow trout (>8 g),Oncorhynchus mykissF,M,T Coppersulfate12 15-18 days LC50 19 - Miller and MacKay 1980Rainbow trout (>8 g),Oncorhynchus mykissF,M,T Coppersulfate99 15-18 days LC50 54 - Miller and MacKay 1980Rainbow trout (>8 g),Oncorhynchus mykissF,M,T Coppersulfate98 15-18 days LC50 78 - Miller and MacKay 1980Rainbow trout (>8 g),Oncorhynchus mykissF,M,T Coppersulfate12 15-18 days LC50 18 - Miller and MacKay 1980Rainbow trout (>8 g),Oncorhynchus mykissF,M,T Coppersulfate97 15-18 days LC50 96 - Miller and MacKay 1980Rainbow trout (200-250 g),Oncorhynchus mykissF,M,T Coppersulfate320 4 mo Altered liver and blood enzymes andmitochondrial function30 - Arillo et al. 1984Rainbow trout (7 cm),Oncorhynchus mykissF,M,T Copperchloride28.4 20 min Avoidance 6.4 - Giattina et al. 1982Rainbow trout (2.70 g),Oncorhynchus mykissF,M,T Copperchloride9.2 96 hr LC50 4.2 - Cusimano et al. 1986Rainbow trout (2.88 g),Oncorhynchus mykissF,M,T Copperchloride9.2 96 hr LC50 66 - Cusimano et al. 1986B-20SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceRainbow trout (2.88 g),Oncorhynchus mykissF,M,T Copperchloride9.2 168 hr LC50 36.7 - Cusimano et al. 1986Rainbow trout (2.70 g),Oncorhynchus mykissF,M,T Copperchloride9.2 168 hr LC50 3.1 - Cusimano et al. 1986Rainbow trout (2.65 g),Oncorhynchus mykissF,M,T Coppersulfate9.2 168 hr LC50 2.3 - Cusimano et al. 1986Rainbow trout (5 day embryo),Oncorhynchus mykissF,M,T Coppersulfate87.7 48 hr LC50 8,000 - Shazili and Pascoe 1986Rainbow trout (10 day embryo),Oncorhynchus mykissF,M,T Coppersulfate87.7 48 hr LC50 2,000 - Shazili and Pascoe 1986Rainbow trout (15 day embryo),Oncorhynchus mykissF,M,T Coppersulfate87.7 48 hr LC50 400 - Shazili and Pascoe 1986Rainbow trout (22 day embryo),Oncorhynchus mykissF,M,T Coppersulfate87.7 48 hr LC50 600 - Shazili and Pascoe 1986Rainbow trout (29 day embryo),Oncorhynchus mykissF,M,T Coppersulfate87.7 48 hr LC50 400 - Shazili and Pascoe 1986Rainbow trout (36 day embryo),Oncorhynchus mykissF,M,T Coppersulfate87.7 48 hr LC50 100 - Shazili and Pascoe 1986Rainbow trout (2 day larva),Oncorhynchus mykissF,M,T Coppersulfate87.7 48 hr LC50 100 - Shazili and Pascoe 1986Rainbow trout (7 day larva),Oncorhynchus mykissF,M,T Coppernitrate87.7 48 hr LC50 100 - Shazili and Pascoe 1986Rainbow trout (yearling),Oncorhynchus mykissF,M,T Coppersulfate63 15 days Olfactory receptor degeneration 20 - Julliard et al. 1993Rainbow trout (swimup),Oncorhynchus mykissF,M,T Coppersulfate60.9 13-40 wk Inhibited olfactory discrimination 20 - Saucier and Astic 1995Rainbow trout (swimup),Oncorhynchus mykissF,M,T Coppersulfate60.9 40 wk 43% mortality 40 - Saucier and Astic 1995Rainbow trout (9.0-11.5 cm,10.6 g),Oncorhynchus mykissF,M,T Coppersulfate284 96 hr LC50 650 - Svecevicius and Vosyliene 1996Rainbow trout (3.5 cm),Oncorhynchus mykissF,M,T Copperchloride24.2 96 hr LC50 12.7 - Marr et al. ManuscriptRainbow trout (3.5 cm),Oncorhynchus mykissF,M,T Copperchloride24.2 96 hr LC50 16.6 - Marr et al. ManuscriptRainbow trout (3.5 cm),Oncorhynchus mykissF,M,T Copperchloride24.2 96 hr LC50 21.4 - Marr et al. ManuscriptRainbow trout (3.5 cm),Oncorhynchus mykissF,M,T Copperchloride24.2 96 hr LC50 34.2 - Marr et al. ManuscriptRainbow trout (10.0 g),Oncorhynchus mykissF,M,D Coppersulfate362 144 hr LC50(extruded diet)276 - Dixon and Hilton 1981Rainbow trout (10.9 g),Oncorhynchus mykissF,M,D Coppersulfate362 144 hr LC50(steam pelleted diet)350 - Dixon and Hilton 1981B-21SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceRainbow trout (12.3 g),Oncorhynchus mykissF,M,D Coppersulfate362 144 hr LC50(Low carbohydrate diet)408 - Dixon and Hilton 1981Rainbow trout (11.6 g),Oncorhynchus mykissF,M,D Coppersulfate362 144 hr LC50(high carbohydrate diet)246 - Dixon and Hilton 1981Rainbow trout (1.7-3.3 g),Oncorhynchus mykissF,M,D Coppersulfate374 21 days Incipient lethal level 329 - Dixon and Sprague 1981aRainbow trout (1.7-3.3 g),Oncorhynchus mykissF,M,D Coppersulfate374 21 days Incipient lethal level 333 - Dixon and Sprague 1981aRainbow trout (1.7-3.3 g),Oncorhynchus mykissF,M,D Coppersulfate374 21 days Incipient lethal level 311 - Dixon and Sprague 1981aRainbow trout (1.7-3.3 g),Oncorhynchus mykissF,M,D Coppersulfate374 21 days Incipient lethal level 274 - Dixon and Sprague 1981aRainbow trout (1.7-3.3 g),Oncorhynchus mykissF,M,D Coppersulfate374 21 days Incipient lethal level 371 - Dixon and Sprague 1981aRainbow trout (1.7-3.3 g),Oncorhynchus mykissF,M,D Coppersulfate374 21 days Incipient lethal level (acclimated to 30ug/L)266 - Dixon and Sprague 1981aRainbow trout (1.7-3.3 g),Oncorhynchus mykissF,M,D Coppersulfate374 21 days Incipient lethal level (acclimated to 58ug/L)349 - Dixon and Sprague 1981aRainbow trout (1.7-3.3 g),Oncorhynchus mykissF,M,D Coppersulfate374 21 days Incipient lethal level (acclimated to 94ug/L)515 - Dixon and Sprague 1981aRainbow trout (1.7-3.3 g),Oncorhynchus mykissF,M,D Coppersulfate374 21 days Incipient lethal level (acclimated to 131ug/L)564 - Dixon and Sprague 1981aRainbow trout (1.7-3.3 g),Oncorhynchus mykissF,M,D Coppersulfate374 21 days Incipient lethal level (acclimated to 194ug/L)708 - Dixon and Sprague 1981aRainbow trout (2.9 g),Oncorhynchus mykissF,M,D Copperchloride30.5 ca. 2 hr Inhibited avoidance of serine 6.667 - Rehnberg and Schreck 1986Rainbow trout (3.2 g),Oncorhynchus mykissF,M,T,D Coppersulfate30 96 hr LC50 - 19.9 Howarth and Sprague 1978Rainbow trout (1.4 g),Oncorhynchus mykissF,M,T,D Coppersulfate101 96 hr LC50 - 176 Howarth and Sprague 1978Rainbow trout (2.2 g),Oncorhynchus mykissF,M,T,D Coppersulfate370 96 hr LC50 - 232 Howarth and Sprague 1978Rainbow trout (smolt),Oncorhynchus mykissF,M,T,D Coppersulfate363 >10 days LC50 97.92 - Fogels and Sprague 1977Rainbow trout (parr),Oncorhynchus mykissF,M,T,D,I - 31.0 62 days NOEC(growth and survival)90 - Mudge et al. 1993Atlantic salmon (2-3 yr parr),Salmo salarS,M,T - 8-10 96 hr LC50 125 - Wilson 1972Atlantic salmon (6.4-11.7 cm),Salmo salarF,M,T Coppersulfate20 7 days LC50 48 - Sprague 1964Atlantic salmon (7.2-10.9 cm),Salmo salarF,M,T - 14 7 days LC50 32 - Sprague and Ramsay 1965Brown trout (3-6 day larva),Salmo truttaS,M,T Copperchloride4 30 days >90% mortality 80 - Reader et al. 1989B-22SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceBrown trout (larva),Salmo truttaS,M,T Copperchloride4 30 days >90% mortality 20 - Sayer et al. 1989Brown trout (larva),Salmo truttaS,M,T Copperchloride22 30 days <10% mortality 80 - Sayer et al. 1989Brown trout (larva),Salmo truttaF,M,T Copperchloride25 60 days Inhibited growth 4.6 - Marr et al. 1996Brook trout,Salvelinus fontinalis- - - 24 hr Significant change in cough rate 9 - Drummond et al. 1973Brook trout (1 g),Salvelinus fontinalisS,M,T Copperchloride4 80 hr 75% mortality 25.4 - Sayer et al. 1991 b, cBrook trout (8 mo),Salvelinus fontinalisR,M,T - 20 10 days IC50(growth)187 - Jop et al. 1995Brook trout (15-20 cm),Salvelinus fontinalisF,M,T Coppersulfate47 21 days Altered Blood Hct, RBC, Hb, Cl,PGOT, Osmolarity, protein38.2 - McKim et al. 1970Brook trout (13-20 cm),Salvelinus fontinalisF,M,T Coppersulfate47 337 days Altered blood PGOT 17.4 - McKim et al. 1970Goldfish (3.8-6.3 cm),Carassius auratusS,U Coppersulfate20 96 hr LC50 36 Pickering and Henderson 1966Goldfish (10.5 g),Carassius auratusS,M,T Coppersulfate34.2 - LC50 150 - Hossain et al. 1995Goldfish (embryo),Carrassius auratusR,U Coppersulfate195 7 days EC50(death or deformity)5,200 - Birge 1978;Birge and Black 1979Goldfish,Carassius auratusR,U Coppersulfate45 24 hr LC50(50C)2,700 - Cairns et al. 1978Goldfish,Carassius auratusR,U Coppersulfate45 24 hr LC50(150C)2,900 - Cairns et al. 1978Goldfish,Carassius auratusR,U Coppersulfate45 24 hr LC50(300C)1,510 - Cairns et al. 1978Common carp (1.8-2.1 cm),Cyprinus carpioS,U Coppersulfate144-188 96 hr LC50 117.5 Deshmukh and Marathe 1980Common carp (5.0-6.0 cm),Cyprinus carpioS,U Coppersulfate144-188 96 hr LC50 530 Deshmukh and Marathe 1980Common carp (embryo),Cyprinus carpioS,U Coppersulfate360 - EC50(hatch and deformity)4,775 - Kapur and Yadav 1982Common carp (embryo),Cyprinus carpioS,U Copperacetate274 96 hr LC50 140 - Kaur and Dhawan 1994Common carp (larva),Cyprinus carpioS,U Copperacetate274 96 hr LC50 4 - Kaur and Dhawan 1994Common carp (fry),Cyprinus carpioS,U Copperacetate274 96 hr LC50 63 - Kaur and Dhawan 1994Common carp,Cyprinus carpioS,M,T Coppernitrate53 - LC50 110 - Rehwoldt et al. 1971Common carp,Cyprinus carpioS,M,T Coppernitrate55 - LC50 800 - Rehwoldt et al. 1972B-23SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceCommon carp (4.7-6.2 cm),Cyprinus carpioR,U Coppersulfate19 96 hr LC50 63 Khangarot et al. 1983Common carp (embryo andlarva),Cyprinus carpioR,U Coppersulfate50 108 hr 77% deformed 10 - Wani 1986Common carp (3.5 cm),Cyprinus carpioR,U Coppersulfate- 96 hr LC50 300 - Alam and Maughan 1992Common carp (6.5 cm),Cyprinus carpioR,U Coppersulfate- 96 hr LC50 1,000 - Alam and Maughan 1992Common carp (embryo),Cyprinus carpioR,M,T Coppersulfate50 72 hr Prevented hatching 700 - Hildebrand and Cushman 1978Common carp (1 mo),Cyprinus carpioR,M,T Coppernitrate84.8 1 wk Raised critical D.O. and alteredammonia excretion14.0 - De Boeck et al. 1995aCommon carp (22.9 cm),Cyprinus carpioF,M,T Copperchloride17 48 hr LC50 170 - Harrison and Rice 1981Common carp (embryo andlarva),Cyprinus carpioF,M,T Copperchloride100 168 hr 55% mortality 19 - Stouthart et al. 1996Common carp (embryo andlarva),Cyprinus carpioF,M,T Copperchloride100 168 hr 18% mortality; 50.8 - Stouthart et al. 1996Bonytail (larva),Gila elegansS, U Coppersulfate199 96 hr LC50 364 Buhl and Hamilton 1996Bonytail (100-110 days),Gila elegansS, U Coppersulfate199 96 hr LC50 231 Buhl and Hamilton 1996Golden shiner (11-13 cm),Notemigonus crysoleucasS,U Coppersulfate221 94 hr Decreased serum osmolality 2,500 - Lewis and Lewis 1971Golden shiner,Notemigonus crysoleucasS,U Coppersulfate45 24 hr LC50(50C)330 - Cairns et al. 1978Golden shiner,Notemigonus crysoleucasS,U Coppersulfate45 24 hr LC50(150C)230 - Cairns et al. 1978Golden shiner,Notemigonus crysoleucasS,U Coppersulfate45 24 hr LC50(300C)270 - Cairns et al. 1978Golden shiner,Notemigonus crysoleucasF,M,T Copperchloride72.2 15 min EC50(avoidance)26 - Hartwell et al. 1989Striped shiner,Notropis chrysocephalusF,M,T,D Coppersulfate318 96 hr LC50 3,400 - Geckler et al. 1976Striped shiner (4.7 cm)Notropis chrysocephalusF,M,T,D Coppersulfate316 96 hr LC50 4,000 - Geckler et al. 1976Striped shiner (5.0 cm)Notropis chrysocephalusF,M,T,D Coppersulfate274 96 hr LC50 5,000 - Geckler et al. 1976Striped shiner,Notropis chrysocephalusF,M,T,D Coppersulfate314 96 hr LC50 8,400 - Geckler et al. 1976B-24SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceStriped shiner,Notropis chrysocephalusF,M,T,D Coppersulfate303 96 hr LC50 16,000 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,U Coppersulfate208 48 hr LC50 290 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,U Coppersulfate132 48 hr LC50 150 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,U Coppersulfate182 48 hr LC50 200 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,U Coppersulfate233 48 hr LC50 180 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,U Coppersulfate282 48 hr LC50 260 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,U Coppersulfate337 48 hr LC50 260 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,U Coppersulfate322 48 hr LC50 6,300 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,U Coppersulfate322 48 hr LC50 11,000 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,U Coppersulfate322 48 hr LC50 25,000 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,U Coppersulfate203 48 hr LC50 160 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,U Coppersulfate203 48 hr LC50 1,100 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,U Coppersulfate203 48 hr LC50 2,900 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate320 48 hr LC50 6,300 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate324 48 hr LC50 9,000 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate324 48 hr LC50 4,700 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate320 48 hr LC50 11,000 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate318 48 hr LC50 5,700 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate318 48 hr LC50 10,000 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate314 48 hr LC50 8,000 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate318 48 hr LC50 11,000 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate324 48 hr LC50 9,700 - Geckler et al. 1976B-25SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceBluntnose minnow,Pimephales notatusS,M,D Coppersulfate339 48 hr LC50 7,000 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate310 48 hr LC50 12,000 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate310 48 hr LC50 21,000 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate302 48 hr LC50 19,000 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate296 48 hr LC50 8,000 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate332 48 hr LC50 11,000 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate340 48 hr LC50 6,300 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate296 48 hr LC50 1,500 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate306 48 hr LC50 750 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate308 48 hr LC50 2,500 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate304 48 hr LC50 1,600 - Geckler et al. 1976Bluntnose minnow,Pimephales notatusS,M,D Coppersulfate315 48 hr LC50 4,000 - Geckler et al. 1976Bluntnose minnow (3.9 cm),Pimephales notatusF,M,T,D Coppersulfate314 96 hr LC50 6,800 - Geckler et al. 1976Bluntnose minnow (5.3 cm),Pimephales notatusF,M,T,D Coppersulfate303 96 hr LC50 13,000 - Geckler et al. 1976Fathead minnow (adult),Pimephales promelasS,U Coppersulfate103-104 96 hr LC50 210Birge et al. 1983Fathead minnow (adult),Pimephales promelasS,U Coppersulfate103-104 96 hr LC50 310Birge et al. 1983Fathead minnow (adult),Pimephales promelasS,U Coppersulfate103-104 96 hr LC50 120Birge et al. 1983Fathead minnow (adult),Pimephales promelasS,U Coppersulfate103-104 96 hr LC50 210 Birge et al. 1983;Benson and Birge 1985Fathead minnow (adult),Pimephales promelasS,U Coppersulfate254-271 96 hr LC50 390 Birge et al. 1983;Benson and Birge 1985Fathead minnow,Pimephales promelasS,U Coppersulfate200 96 hr LC50 430 Mount 1968Fathead minnow,Pimephales promelasS,U Coppersulfate31 96 hr LC50 84 Mount and Stephan 1969Fathead minnow (3.8-6.3 cm),Pimephales promelasS,U Coppersulfate20 96 hr LC50 25 Pickering and Henderson 1966B-26SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceFathead minnow (3.8-6.3 cm),Pimephales promelasS,U Coppersulfate20 96 hr LC50 23 Pickering and Henderson 1966Fathead minnow (3.8-6.3 cm),Pimephales promelasS,U Coppersulfate20 96 hr LC50 23 Pickering and Henderson 1966Fathead minnow (3.8-6.3 cm),Pimephales promelasS,U Coppersulfate20 96 hr LC50 22 Pickering and Henderson 1966Fathead minnow (3.8-6.3 cm),Pimephales promelasS,U Coppersulfate360 96 hr LC50 1760 Pickering and Henderson 1966Fathead minnow (3.8-6.3 cm),Pimephales promelasS,U Coppersulfate360 96 hr LC50 1140 Pickering and Henderson 1966Fathead minnow,Pimephales promelasS,U Coppersulfate20 96 hr LC50 50 Tarzwell and Henderson 1960Fathead minnow,Pimephales promelasS,U Coppersulfate400 96 hr LC50 1,400 Tarzwell and Henderson 1960Fathead minnow (3.2-4.2 cm),Pimephales promelasS,M Copperacetate44 96 hr LC50 117 - Curtis et al. 1979;Curtis and Ward 1981Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate294 96 hr LC50 16,000 - Brungs et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate120 96 hr LC50 2,200 - Brungs et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate298 96 hr LC50 16,000 - Brungs et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate280 96 hr LC50 3,300 - Brungs et al. 1976;Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate244 96 hr LC50 1,600 - Brungs et al. 1976;Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate212 96 hr LC50 2,000 - Brungs et al. 1976;Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate260 96 hr LC50 3,500 - Brungs et al. 1976;Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate224 96 hr LC50 9,700 - Brungs et al. 1976;Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate228 96 hr LC50 5,000 - Brungs et al. 1976;Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate150 96 hr LC50 2,800 - Brungs et al. 1976;Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate310 96 hr LC50 11,000 - Brungs et al. 1976;Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate280 96 hr LC50 12,000 - Brungs et al. 1976;Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate280 96 hr LC50 11,000 - Brungs et al. 1976;Geckler et al. 1976B-27SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceFathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate260 96 hr LC50 22,200 - Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate308 96 hr LC50 4,670 - Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate206 96 hr LC50 920 - Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate262 96 hr LC50 1,190 - Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate322 96 hr LC50 2,830 - Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate210 96 hr LC50 1,450 - Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate260 96 hr LC50 1,580 - Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate252 96 hr LC50 1,000 - Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate312 96 hr LC50 5,330 - Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate276 96 hr LC50 4,160 - Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate252 96 hr LC50 10,550 - Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate298 96 hr LC50 22,200 - Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate282 96 hr LC50 21,800 - Geckler et al. 1976Fathead minnow (2.0-6.9 cm),Pimephales promelasS,M,D Coppersulfate284 96 hr LC50 23,600 - Geckler et al. 1976Fathead minnow (<24 h),Pimephales promelasS,M,T Coppernitrate290 96 hr LC50 >200 - Schubauer-Berigan et al. 1993Fathead minnow (<24 h),Pimephales promelasS,M,T Coppersulfate16.8 96 hr LC50 36.0 - Welsh et al. 1993Fathead minnow (<24 h),Pimephales promelasS,M,T Coppersulfate19.0 96 hr LC50 70.3 - Welsh et al. 1993Fathead minnow (<24 h),Pimephales promelasS,M,T Coppersulfate19.0 96 hr LC50 85.6 - Welsh et al. 1993Fathead minnow (<24 h),Pimephales promelasS,M,T Coppersulfate19.0 96 hr LC50 182.0 - Welsh et al. 1993Fathead minnow (<24 h; 0.68mg),Pimephales promelasS,M,T Coppersulfate17 96 hr LC50 1.99 - Welsh et al. 1993B-28SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceFathead minnow (<24 h; 0.68mg),Pimephales promelasS,M,T Coppersulfate20.5 96 hr LC50 4.86 - Welsh et al. 1993Fathead minnow (<24 h; 0.68mg),Pimephales promelasS,M,T Coppersulfate16.5 96 hr LC50 11.1 - Welsh et al. 1993Fathead minnow (<24 h; 0.68mg),Pimephales promelasS,M,T Coppersulfate17.5 96 hr LC50 9.87 - Welsh et al. 1993Fathead minnow (<24 h; 0.68mg),Pimephales promelasS,M,T Coppersulfate17 96 hr LC50 15.7 - Welsh et al. 1993Fathead minnow (60-90 days),Pimephales promelasS,M,T - 110 48 hr LC50 284 - Dobbs et al. 1994Fathead minnow (3 wk),Pimephales promelasS,M,T Coppersulfate101 48 hr Short-term intolerance of hypoxia (2mg D.O./L)186 - Bennett et al. 1995Fathead minnow (2-4 day),Pimephales promelasS,M,T Coppersulfate6-10 - LC50 12.5 - Suedel et al. 1996Fathead minnow (<24 hrs),Pimephales promelasS,M,T Coppersulfate9.9 96 hr LC50 10.7 - Welsh et al. 1996Fathead minnow (<24 hrs),Pimephales promelasS,M,T Coppersulfate7.1 96 hr LC50 6.3 - Welsh et al. 1996Fathead minnow (<24 hrs),Pimephales promelasS,M,T Coppersulfate8.3 96 hr LC50 12.2 - Welsh et al. 1996Fathead minnow (<24 hrs),Pimephales promelasS,M,T Coppersulfate8.9 96 hr LC50 9.5 - Welsh et al. 1996Fathead minnow (<24 hrs),Pimephales promelasS,M,T Coppersulfate16.8 96 hr LC50 26.8 - Welsh et al. 1996Fathead minnow (<24 hrs),Pimephales promelasS,M,T Coppersulfate12.2 96 hr LC50 21.2 - Welsh et al. 1996Fathead minnow (<24 hrs),Pimephales promelasS,M,T Coppersulfate9.4 96 hr LC50 19.8 - Welsh et al. 1996Fathead minnow (<24 hrs),Pimephales promelasS,M,T Coppersulfate11.4 96 hr LC50 31.9 - Welsh et al. 1996Fathead minnow (<24 hrs),Pimephales promelasS,M,T Coppersulfate10.9 96 hr LC50 26.1 - Welsh et al. 1996Fathead minnow (<24 hrs),Pimephales promelasS,M,T Coppersulfate12.4 96 hr LC50 26.0 - Welsh et al. 1996Fathead minnow (<24 hrs),Pimephales promelasS,M,T Coppersulfate17.4 96 hr LC50 169.5 - Welsh et al. 1996Fathead minnow (<24 hrs),Pimephales promelasS,M,T,D Coppersulfate46 96 hr LC50 17.15 14.87 Erickson et al. 1996a,bFathead minnow (<24 hrs),Pimephales promelasS,M,T,D Coppersulfate46 96 hr LC50 21.59 18.72 Erickson et al. 1996a,bB-29SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceFathead minnow (<24 hrs),Pimephales promelasS,M,T,D Coppersulfate47 96 hr LC50 123.19 106.8 Erickson et al. 1996a,bFathead minnow (<24 hrs),Pimephales promelasS,M,T,D Coppersulfate45 96 hr LC50 42.56 36.89 Erickson et al. 1996a,bFathead minnow (<24 hrs),Pimephales promelasS,M,T,D Coppersulfate46 96 hr LC50 83.19 72.13 Erickson et al. 1996a,bFathead minnow,Pimephales promelasS,M,T,D Coppersulfate100 96 hr LC50 (fish from metal-contaminatedpond)360 - Birge et al. 1983Fathead minnow,Pimephales promelasS,M,T,D Coppersulfate250 96 hr LC50 (fish from metal-contaminatedpond)410 - Birge et al. 1983Fathead minnow (<24 hr),Pimephales promelasR,U - 45 7 days LC50 70 - Norberg and Mount 1985Fathead minnow (<24 hr),Pimephales promelasR,U - 45 7 days LOEC(growth)26 - Norberg and Mount 1985Fathead minnow (<24 hr),Pimephales promelasR,U Coppersulfate345 4 days RNA threshhold effect 130 - Parrott and Sprague 1993Fathead minnow (embryo),Pimephales promelasR,U Coppersulfate106 5 days LC50 480 - Fort et al. 1996Fathead minnow (embryo),Pimephales promelasR,U Coppersulfate106 5 days LC50 440 - Fort et al. 1996Fathead minnow (embryo),Pimephales promelasR,U Coppersulfate106 5 days EC50(malformation)270 - Fort et al. 1996Fathead minnow (embryo),Pimephales promelasR,U Coppersulfate106 5 days EC50(malformation)260 - Fort et al. 1996Fathead minnow (embryo),Pimephales promelasR,U Coppersulfate106 7 days LC50 310 - Fort et al. 1996Fathead minnow (embryo),Pimephales promelasR,U Coppersulfate106 7 days LC50 330 - Fort et al. 1996Fathead minnow (embryo),Pimephales promelasR,U Coppersulfate106 7 days EC50(malformation)190 - Fort et al. 1996Fathead minnow (embryo),Pimephales promelasR,U Coppersulfate106 7 days EC50(malformation)170 - Fort et al. 1996Fathead minnow (embryo),Pimephales promelasR,U Coppersulfate106 7 days LOEC(length)160 - Fort et al. 1996Fathead minnow (embryo),Pimephales promelasR,U Coppersulfate106 7 days LOEC(length)180 - Fort et al. 1996Fathead minnow (larva),Pimephales promelasR,M,T Coppersulfate180 7 days LOEC(growth)25 - Pickering and Lazorchak 1995Fathead minnow (larva),Pimephales promelasR,M,T Coppersulfate218 7 days LOEC(growth)38 - Pickering and Lazorchak 1995Fathead minnow (larva),Pimephales promelasR,M,T Coppersulfate218 7 days LOEC(growth)38 - Pickering and Lazorchak 1995Fathead minnow (3-7 days),Pimephales promelasR,M,T Coppersulfate74 48 hr LC50 225 - Diamond et al. 1997bB-30SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceFathead minnow (larva),Pimephales promelasR,M,T,D Coppersulfate80 48 hr LC50 35.9 - Diamond et al. 1997aFathead minnow (larva),Pimephales promelasR,M,T,D Coppersulfate80 48 hr LC50 28.9 - Diamond et al. 1997aFathead minnow (larva),Pimephales promelasR,M,T,D Coppersulfate80 48 hr LC50 20.7 - Diamond et al. 1997aFathead minnow (larva),Pimephales promelasR,M,T,D Coppersulfate80 48 hr LC50 80.8 - Diamond et al. 1997aFathead minnow (3-7 days),Pimephales promelasR,M,T,D Coppersulfate80 48 hr LC50 297.1 - Diamond et al. 1997bFathead minnow (3-7 days),Pimephales promelasR,M,T,D Coppersulfate72 48 hr LC50 145.8 - Diamond et al. 1997bFathead minnow (32-38 mm),Pimephales promelasF,M,T Coppersulfate244 9 mo LOEC(93% lower fecundity)120 - Brungs et al. 1976Fathead minnow (larva),Pimephales promelasF,M,T Coppersulfate202 - LC50 250 - Scudder et al. 1988Fathead minnow (embryo),Pimephales promelasF,M,T Coppersulfate202 34 days Reduced growth;increased abnormality61 - Scudder et al. 1988Fathead minnow (embryo),Pimephales promelasF,M,T Coppersulfate202 34 days LC50 123 - Scudder et al. 1988Fathead minnow (24-96 hr),Pimephales promelasF,M,T Coppersulfate10.7 21 days Incipient lethal level 6.2 - Welsh 1996Fathead minnow (24-96 hr),Pimephales promelasF,M,T Coppersulfate10.7 21 days Growth (length) reduced by 8% 5.3 - Welsh 1996Fathead minnow (24-96 hr),Pimephales promelasF,M,T Coppersulfate9.3 21 days Incipient lethal level 17.2 - Welsh 1996Fathead minnow (24-96 hr),Pimephales promelasF,M,T Coppersulfate9.3 21 days Growth (length) reduced by 17% 16.2 - Welsh 1996Fathead minnow (<24 hrs),Pimephales promelasF,M,T Coppersulfate46 96 hr LC50 305 - Erickson et al. 1996 a,bFathead minnow (<24 hrs),Pimephales promelasF,M,T Coppersulfate46 96 hr LC50 298.6 - Erickson et al. 1996 a, bFathead minnow,Pimephales promelasF,M,T - 30 96 hr LC50(TOC=12 mg/L)436 - Lind et al. manuscriptFathead minnow,Pimephales promelasF,M,T - 37 96 hr LC50(TOC=13 mg/L)516 - Lind et al. manuscriptFathead minnow,Pimephales promelasF,M,T - 87 96 hr LC50(TOC=36 mg/L)1,586 - Lind et al. manuscriptFathead minnow,Pimephales promelasF,M,T - 73 96 hr LC50(TOC=28 mg/L)1,129 - Lind et al. manuscriptFathead minnow,Pimephales promelasF,M,T - 84 96 hr LC50(TOC=15 mg/L)550 - Lind et al. manuscriptFathead minnow,Pimephales promelasF,M,T - 66 96 hr LC50(TOC=34 mg/L)1,001 - Lind et al. manuscriptB-31SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceFathead minnow,Pimephales promelasF,M,T - 117 96 hr LC50(TOC=30 mg/L)2,050 - Lind et al. manuscriptFathead minnow,Pimephales promelasF,M,T - 121 96 hr LC50(TOC=30 mg/L)2,336 - Lind et al. manuscriptFathead minnow,Pimephales promelasF,M,T Coppersulfate117 96 hr LC50 2,050 - Lind et al. manuscriptFathead minnow,Pimephales promelasF,M,T Coppersulfate121 96 hr LC50 2,336 - Lind et al. manuscriptFathead minnow (4.4 cm),Pimephales promelasF,M,T,D Coppersulfate314 96 hr LC50 11,000 - Geckler et al. 1976Fathead minnow (4.2 cm),Pimephales promelasF,M,T,D Coppersulfate303 96 hr LC50 15,000 - Geckler et al. 1976Fathead minnow (<24 hrs),Pimephales promelasF,M,T,D Coppersulfate45 96 hr LC50 158.8 138.1 Erickson et al. 1996a,bFathead minnow (<24 hrs),Pimephales promelasF,M,T,D Coppersulfate45 96 hr LC50 80.01 72.01 Erickson et al. 1996a,bFathead minnow (<24 hrs),Pimephales promelasF,M,T,D Coppersulfate46 96 hr LC50 20.96 18.23 Erickson et al. 1996a,bFathead minnow (<24 hrs),Pimephales promelasF,M,T,D Coppersulfate44 96 hr LC50 50.8 39.12 Erickson et al. 1996a,bFathead minnow (<24 hrs),Pimephales promelasF,M,T,D Coppersulfate45 96 hr LC50 65.41 45.78 Erickson et al. 1996a,bColorado squawfish (larva),Ptychocheilus luciusS,U Coppersulfate199 96 hr LC50 363 Buhl and Hamilton 1996Colorado squawfish (155-186days),Ptychocheilus luciusS,U Coppersulfate199 96 hr LC50 663 Buhl and Hamilton 1996Colorado squawfish (32-40 daysposthatch),Ptychocheilus luciusS,U Coppersulfate144 96 hr LC50 293 Hamilton and Buhl 1997Colorado squawfish (32-40 daysposthatch),Ptychocheilus luciusS,U Coppersulfate144 96 hr LC50 320 Hamilton and Buhl 1997Creek chub,Semotilus atromaculatusF,M,T Coppersulfate316 96 hr LC50 11,500 - Geckler et al. 1976Creek chub,Semotilus atromaculatusF,M,T Coppersulfate274 96 hr LC50 1,100 - Geckler et al. 1976Razorback sucker (larva),Xyrauchen texanusS,U Coppersulfate199 96 hr LC50 404 Buhl and Hamilton 1996Razorback sucker (102-116days),Xyrauchen texanusS,U Coppersulfate199 96 hr LC50 331 Buhl and Hamilton 1996Razorback sucker (13-23 daysposthatch),Xyrauchen texanusS,U Coppersulfate144 96 hr LC50 231 Hamilton and Buhl 1997B-32SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceRazorback sucker (13-23 daysposthatch),Xyrauchen texanusS,U Coppersulfate144 96 hr LC50 314 Hamilton and Buhl 1997Brown bullhead,Ictallurus nebulosusF,M,T Coppersulfate303 96 hr LC50 12,000 - Geckler et al. 1976Brown bullhead (5.2 cm),Ictalurus nebulosusF,M,T Coppersulfate314 96 hr LC50 5,200 - Geckler et al. 1976Channel catfish (13-14 cm),Ictalurus punctatusS,U Coppersulfate221 94 hr Decreased serum osmolality 2,500 - Lewis and Lewis 1971Channel catfish,Ictalurus punctatusS,U Coppersulfate45 24 hr LC50(50C)3,700 - Cairns et al. 1978Channel catfish,Ictalurus punctatusS,U Coppersulfate45 24 hr LC50(150C)2,600 - Cairns et al. 1978Channel catfish,Ictalurus punctatusS,U Coppersulfate45 24 hr LC50(300C)3,100 - Cairns et al. 1978Channel catfish,Ictalurus punctatusS,U Coppersulfate100 10 days EC50(death and deformity)6,620 - Birge and Black 1979Channel catfish (fingerlings),Ictalurus punctatusS,U Coppersulfate16 96 hr LC50 54 Straus and Tucker 1993Channel catfish (fingerlings),Ictalurus punctatusS,U Coppersulfate16 96 hr LC50 55 Straus and Tucker 1993Channel catfish (fingerlings),Ictalurus punctatusS,U Coppersulfate83 96 hr LC50 762 Straus and Tucker 1993Channel catfish (fingerlings),Ictalurus punctatusS,U Coppersulfate83 96 hr LC50 700 Straus and Tucker 1993Channel catfish (fingerlings),Ictalurus punctatusS,U Coppersulfate161 96 hr LC50 768 Straus and Tucker 1993Channel catfish (fingerlings),Ictalurus punctatusS,U Coppersulfate161 96 hr LC50 1139 Straus and Tucker 1993Channel catfish (fingerlings),Ictalurus punctatusS,U Coppersulfate287 96 hr LC50 1041 Straus and Tucker 1993Channel catfish (fingerlings),Ictalurus punctatusS,U Coppersulfate287 96 hr LC50 925 Straus and Tucker 1993Channel catfish (400-600 g),Ictalurus punctatusF,M,T Coppersulfate- 10 wk Significant mortality 354 - Perkins et al. 1997Channel catfish (4.1 gm),Ictalurus punctatusF,M,T,D Coppersulfate319 14 days LC50 1,229 - Richey and Roseboom 1978Channel catfish (5.7 gm),Ictalurus punctatusF,M,T,D Coppersulfate315 14 days LC50 1,073 - Richey and Roseboom 1978Banded killifish,Fundulus diaphanusS,M,T Coppernitrate53 - 860 - Rehwoldt et al. 1971Banded killifish,Fundulus diaphanusS,M,T Coppernitrate55 - 840 - Rehwoldt et al. 1972B-33SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceFlagfish (0.1-0.3 g),Jordanella floridaeF,M,T,D Coppersulfate363 10 days LC50 - 680 Fogels and Sprague 1977Flagfish (0.1-0.3 g),Jordanella floridaeF,M,T,D Coppersulfate363 96 hr LC50 - 1,270 Fogels and Sprague 1977Mosquitofish (3.8-5.1 cmfemale),Gambusia affinisS,U Coppernitrate27-41 96 hr LC50 93 Joshi and Rege 1980Mosquitofish (3.8-5.1 cmfemale),Gambusia affinisS,U Coppersulfate27-41 96 hr LC50 200 Joshi and Rege 1980Mosquitofish (2.5 cm male),Gambusia affinisS,U - 50 96 hr LC50 3,500 Kallanagoudar and Patil 1997Mosquitofish (2.5 cm male),Gambusia affinisS,U - 150 96 hr LC50 5,000 Kallanagoudar and Patil 1997Mosquitofish (2.5 cm male),Gambusia affinisS,U - 300 96 hr LC50 6,000 Kallanagoudar and Patil 1997Mosquitofish (3.5 cm female),Gambusia affinisS,U - 50 96 hr LC50 2,500 Kallanagoudar and Patil 1997Mosquitofish (3.5 cm female),Gambusia affinisS,U - 150 96 hr LC50 2,900 Kallanagoudar and Patil 1997Mosquitofish (3.5 cm female),Gambusia affinisS,U - 300 96 hr LC50 5,000 Kallanagoudar and Patil 1997Mosquitofish (0.8 cm fry),Gambusia affinisS,U - 50 96 hr LC50 900 Kallanagoudar and Patil 1997Mosquitofish (0.8 cm fry),Gambusia affinisS,U - 150 96 hr LC50 1,400 Kallanagoudar and Patil 1997Mosquitofish (0.8 cm fry),Gambusia affinisS,U - 300 96 hr LC50 2,000 Kallanagoudar and Patil 1997Mosquito fish,Gambusia affinisS,U Coppersulfate- 96 hr LC50(high turbidity)75,000 - Wallen et al. 1957Mosquito fish,Gambusia affinisR,M Coppersulfate45 48 hr LC50 180 - Chagnon and Guttman 1989Guppy (1.5 cm),Poecilia reticulataS,U Coppersulfate230 96 hr LC50 1,230 Khangarot 1981Guppy (1.62 cm),Poecilia reticulataS,U Coppersulfate240 96 hr LC50 764 Khangarot et al. 1981bGuppy (1.9-2.5 cm),Poecilia reticulataS,U Coppersulfate20 96 hr LC50 36 Pickering and Henderson 1966Guppy (1.5 cm),Poecilia reticulataR,U Coppersulfate260 96 hr LC50 2,500 Khangarot et al. 1981aGuppy (0.8-1.0 cm),Poecilia reticulataR,U Coppersulfate144-188 96 hr LC50 160 Deshmukh and Marathe 1980Guppy (1.2-2.3 cm; female),Poecilia reticulataR,U Coppersulfate144-188 96 hr LC50 275 Deshmukh and Marathe 1980B-34SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceGuppy (2.3-2.8 cm; male),Poecilia reticulataR,U Coppersulfate144-188 96 hr LC50 210 Deshmukh and Marathe 1980Guppy (340 mg; female),Poecilia reticulataR,U Coppersulfate144-188 96 hr LC50 480 Deshmukh and Marathe 1980Guppy (1.5 cm),Poecilia reticulataR,U Coppersulfate260 48 hr LC50 2,500 - Khangarot et al. 1981aGuppy (1.5 cm),Poecilia reticulataR, U Coppersulfate181 96 hr LC50 986 - Khangarot and Ray 1987bGuppy (1 mo),Poecilia reticulataF,U Coppersulfate76 24 hr LC50 1,370 - Minicucci 1971Guppy (1 mo),Poecilia reticulataF,U Coppersulfate76 24 hr LC50 930 - Minicucci 1971Guppy (1 mo),Poecilia reticulataF,U Coppersulfate76 24 hr LC50 1,130 - Minicucci 1971White perch,Morone americanaS,M,T Coppernitrate53 - LC50 6,200 - Rehwoldt et al. 1971White perch,Morone americanaS,M,T Coppernitrate55 - LC50 6,400 - Rehwoldt et al. 1972Striped bass (larva),Morone saxitilisS,U Copperchloride34.6 96 hr LC50 50 Hughes 1973Striped bass (larva),Morone saxitilisS,U Coppersulfate34.6 96 hr LC50 100 Hughes 1973Striped bass (3.5-5.1 cm),Morone saxitilisS,U Copperchloride34.6 96 hr LC50 50 Hughes 1973Striped bass (3.1-5.1 cm),Morone saxitilisS,U Coppersulfate34.6 96 hr LC50 150 Hughes 1973Striped bass (35-80 day),Morone saxitilisS,U Coppersulfate285 96 hr LC50 270 Palawski et al. 1985Striped bass (6 cm),Morone saxitilisS,U Coppersulfate35 96 hr LC50 620 Wellborn 1969Striped bass,Morone saxitilisS,M,T Coppernitrate53 96 hr LC50 4,300 - Rehwoldt et al. 1971Striped bass,Morone saxitilisS,M,T Coppernitrate55 96 hr LC50 2,700 - Rehwoldt et al. 1972Rock bass,Ambloplites rupestrisF,M,T - 24 96 hr LC50(high TOC)1,432 - Lind et al. manuscriptPumpkinseed (1.2 g),Lepomis gibbosusS,M,T Coppernitrate53 - LC50 2,400 - Rehwoldt et al. 1971Pumpkinseed (1.2 g),Lepomis gibbosusS,M,T Coppernitrate55 - LC50 2,700 - Rehwoldt et al. 1972Pumpkinseed,Lepomis gibbosusS,M,T Coppernitrate53 96 hr LC50 2,400 - Rehwoldt et al. 1971Pumpkinseed,Lepomis gibbosusS,M,T Coppernitrate55 96 hr LC50 2,700 - Rehwoldt et al. 1972B-35SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceBluegill,Lepomis macrochirusS,U Copperchloride43 96 hr LC50 770 Academy of Natural Sciences 1960Bluegill,Lepomis macrochirusS,U Coppersulfate43 96 hr LC50 1,250Academy of Natural Sciences 1960Cairns and Scheier 1968; Patrick etBluegill,Lepomis macrochirusS,U Coppersulfate45 24 hr LC50(50C)2,590 - Cairns et al. 1978Bluegill,Lepomis macrochirusS,U Coppersulfate45 24 hr LC50(150C)2,500 - Cairns et al. 1978Bluegill,Lepomis macrochirusS,U Coppersulfate45 24 hr LC50(300C)3,820 - Cairns et al. 1978Bluegill (3-4 cm),Lepomis macrochirusS,U - 119 8 days 33% reduction in locomotor activity 40 - Ellgaard and Guillot 1988Bluegill (4.2 cm),Lepomis macrochirusS,U Coppersulfate52 96 hr LC50 254 Inglis and Davis 1972Bluegill (4.2 cm),Lepomis macrochirusS,U Coppersulfate209 96 hr LC50 437 Inglis and Davis 1972Bluegill (4.2 cm),Lepomis macrochirusS,U Coppersulfate365 96 hr LC50 648 Inglis and Davis 1972Bluegill (5-15 g),Lepomis macrochirusS,U Coppersulfate35 2-6 days 8% increase in oxygen consumptionrates300 - O'Hara 1971Bluegill (3.8-6.3 cm),Lepomis macrochirusS,U Coppersulfate20 96 hr LC50 660 Pickering and Henderson 1966Bluegill (3.8-6.3 cm),Lepomis macrochirusS,U Coppersulfate360 96 hr LC50 10,200 Pickering and Henderson 1966Bluegill,Lepomis macrochirusS,U Coppersulfate20 96 hr LC50 200 Tarzwell and Henderson 1960Bluegill,Lepomis macrochirusS,U Coppersulfate400 96 hr LC50 10,000 Tarzwell and Henderson 1960Bluegill (5-11 cm),Lepomis macrochirusS,U Coppersulfate46 48 hr LC50 3,000 - Turnbull et al. 1954Bluegill (5-11 cm),Lepomis macrochirusS,U Coppersulfate101.2 48 hr LC50 7,000 - Turnbull et al. 1954Bluegill (0.51g),Lepomis macrochirusS,M,T - 110 48 hr LC50 4,300 - Dobbs et al. 1994B-36SpeciesMethodaChemicalHardness(mg/L asCaCO3)Duration EffectTotalConcentration(μg/L)bDissolvedConcentration(μg/L)ReferenceBluegill (5-9 cm),Lepomis macrochirusS,M,T Copperchloride45-47 - LC50 710 - Trama 1954Bluegill (5-9 cm),Lepomis macrochirusS,M,T Coppersulfate45-47 - LC50 770 - Trama 1954Bluegill (5-15 g),Lepomis macrochirusF,M Coppersulfate35 - LC50 2400 - O'Hara 1971Bluegill (3.5-6.0 cm),Lepomis macrochirusF,M,T Coppersulfate112.4 80 min Avoidance threshold 8,480 - Black and Birge 1980Bluegill (3.2-6.7 cm),Lepomis macrochirusF,M,T Copperchloride21.2-59.2 96 hr LC50 1,100 - Thompson et al. 1980Bluegill (3.2-6.7 cm),Lepomis macrochirusF,M,T Copperchloride21.2-59.2 96 hr LC50 900 - Thompson et al. 1980Bluegill (35.6-62.3 g),Lepomis macrochirusF,M,T Coppersulfate273.3 24-96 hr Various behavioral changes 34 - Henry and Atchison 1986Bluegill,Lepomis macrochirusF,M,T Copperchloride157 24-96 hr 27% reduction in food consumption 31 - Sandheinrich and Atchison 1989Bluegill,Lepomis macrochirusF,M,T,D Coppersulfate316 96 hr LC50(high BOD)16,000 - Geckler et al. 1976Bluegill,Lepomis macrochirusF,M,T,D Coppersulfate318 96 hr LC50 (high BOD) 17,000 - Geckler et al. 1976Bluegill (0.14-0.93 g),Lepomis macrochirusF,M,T,D Coppersulfate246 14 days LC50 - 2,500 Richey and Roseboom 1978Bluegill (1.15-2.42 g),Lepomis macrochirusF,M,T,D Coppersulfate237 14 days LC50 - 3,700 Richey and Roseboom 1978Bluegill (48.3 g),Lepomis macrochirusF,M,T,D Coppersulfate40 96 hr Biochemical changes 2,000 - Heath 1984Largemouth bass (embryo),Micropterus salmoidesR,U Coppersulfate100 8 days EC50(death and deformity)6,560 - Birge et al. 1978; Birge and Black1979Largemouth bass,Micropterus salmoidesF,U - - 24 hr Affected opercular rhythm 48 - Morgan 1979Rainbow darter,Etheostoma caeruleumF,M,T,D Coppersulfate318 96 hr LC50(high BOD)4,500 - Geckler et al. 1976Rainbow darter,Etheostoma caeruleumF,M,T,D Coppersulfate316 96 hr LC50(high BOD)8,000 - Geckler et al. 1976Rainbow darter,Etheostoma caeruleumF,M,T,D Coppersulfate274 96 hr LC50(high BOD)2,800 - Geckler et al. 1976Rainbow darter (4.6 cm),Etheostoma caeruleumF,M,T,D Coppersulfate314 96 hr LC50 (high BOD) 4,800 - Geckler et al. 1976Rainbow darter (4.6 cm),Etheostoma caeruleumF,M,T,D Coppersulfate303 96 hr LC50 (high BOD) 5,300 - Geckler et al. 1976Fantail,Etheostoma flabellareS,M,T Coppersulfate170 96 hr Lowered critical thermal maximum 43 - Lydy and Wissing 1988B-37

# Appendix C. Estimation of Water Chemistry Parameters for Acute Copper Toxicity Tests

# FINAL REPORT

ESTIMATION OF WATER CHEMISTRY PARAMETERS FOR

ACUTE COPPER TOXICITY TESTS

For:

U.S. Environmental Protection Agency
Health and Ecological Criteria Division
Office of Science and Technology, Office of Water
1200 Pennsylvania Avenue, NW
Washington, DC 20460

Great Lakes Environmental Center Program Manager

G.M. DeGraeve

Great Lakes Environmental Center, Inc.
739 Hastings Street, Traverse City, Michigan 49686

Phone: (231) 941-2230

### C -1 

## CONTENTS

Foreword

.................................................................... C -3

1.0 Data Acquisition

............................................................ C -4

2.0 Technical Issues and Corresponding Recommendations

.................................. C -4

2.1 Estimating Ion Concentrations

............................................. C -4

2.2 pH Adjustment with HCl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -7

2.3 Estimation of DOC

..................................................... C -7

2.4 DOC in Lake Superior Water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -8

2.5 Applying Water Chemistry Data to Lake Superior Water . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -8

2.6 Predicting Ionic Composition of WFTS Well Water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -9

2.7 Data for Measurement of Blacksburg/New River Water . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -11

2.8 Cu Concentrations and Alkalinity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -12

2.9 Calculation of DOC and Humic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -14

2.10 Alkalinity of Lake Superior Water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -15

2.11 Availability of LC50s

.................................................. C -15

2.12 Cl and Na Concentrations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -15

2.13 Calculating DOC in Dilution Water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -16

2.14 Ionic Composition of Chehalis River Water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -16

2.15 Chemistry of Water in Howarth and Sprague (1978)

.............................. C -17

2.16 Default Values of Analyte Concentrations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -18

2.17 Organic Carbon Content of Samples

......................................... C -19

2.18 Additional Water Chemistry Data Needed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -19

2.19 Estimating Data for Waters

............................................... C -19

References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -32

Appendix C -1, Calculations for Ionic Composition of Standard Laboratory Reconstituted

Water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -35
Appendix C -2, Dissolved, Particulate, and Estimated Total Organic Car bon for Streams and Lakes by State . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -36

Appendix C -3, Mean TOC and DOC in Lake Superior Dilution Water . . . . . . . . . . . . . . . . . . . . . . . . . . C -38
Appendix C -4, Measured Hardness and Major Ion and Cation Concentrations in WFTS Well Water from April 1972 to April 1978

................................ C -39

Appendix C -5, Analytical Results of New and Clinch Rivers and Sinking Creek, VA, Water Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -41

Appendix C -6, Water Composition of St. Louis River, MN, from USGS NASQAN and Select
Relationships to Water Hardness . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -42
Appendix C -7, Supplementary Data for Bennett et al. (1995)

................................. C -48
Appendix C -8, Supplementary Data for Richards and Beitinger (1995)

.......................... C -51
Appendix C -9, Water Quality Data for the American River, CA for July 1978 Through D ecember 1980

...................................... C -52

Appendix C -10, STORET Data for Minnesota Lakes and Rivers

.............................. C -53

### C -2 

## TABLES

Table 1. Standard Reconstituted Water Composition and Target Water Quality Characteristics

......... C -5

Table 2. Calculated Ion Concentrations Based on the Standard Salts Added

...................... C -6

Table 3. Adjusted Ion Concentrations for a Standard Reconstituted Water Mix Based on Reported

Hardness

............................................................. C -6

Table 4. Recommended Spreadsheet Addition for Lake Superior Dilution Water . . . . . . . . . . . . . . . . . . . C -9

Table 5. Predicted Ion Concentrations in WFTS Well Water Based on Measured Hardness . . . . . . . . . . . C -10

Table 6. Comparison of Values for Untreated (Natural) and Treated (Dechlorinated City of
Blacksburg, VA) New River Water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -13

Table 7. Estimated Alkalinity in Natural Surf ace Water Based on pH

.......................... C -14

Table 8. Estimates of Dissolved Organic Car bon and Percent Humic Acid for the Winner (1985)
Toxicity Tests

........................................................ C -14

Table 9. Example Calculations to Estimate Water Chemistry of Tests C onducted at 100 mg/L as

CaCo 3 

## by Howarth and Sprague (1978) Using a Mixture of University of Guelph Well

Water and De-ionized Water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C -18

FIGURES

Figure 1. Relationship Between Ca and Hardness in WFTS Well Water

........................ C -24

Figure 2. Relationship Between Mg and Hardness in WFTS Well Water . . . . . . . . . . . . . . . . . . . . . . . C -25

Figure 3. Relationship Between Na and Hardness in WFTS Well Water . . . . . . . . . . . . . . . . . . . . . . . . C -26

Figure 4. Relationship Between K and Hardness in WFTS Well Water

........................ C -27

Figure 5. Relationship Between Cl and Hardness in WFTS Well Water . . . . . . . . . . . . . . . . . . . . . . . . C -28

Figure 6. Relationship Between SO 4 

## and Hardness in WFTS Well Water . . . . . . . . . . . . . . . . . . . . . . . C -29

Figure 7. Slopes of the Regression Equations Derived for Na Concentration in St. Louis River, MN,

Water Versus Water Hardness from 1973 to 1993

................................. C -30

Figure 8. Intercepts of the Regression Equations Derived for Na Concentration in St. Louis River,

MN, Water Versus Water Hardness from 1973 to 1993

............................. C -31

### C -3 

## FOREWORD

This report was developed by the Great Lakes Environmental Center. Some minor revisions

were made by the U.S. Environmental Protection Agency (EPA). These revisions were primarily
editorial. Additional editorial and formatting revisions were made by the CDM Group, Inc.

The purpose of this report is to provide input water chemistry information for a Biotic Ligand

Model (BLM) analysis of the acute copper toxicity data in Table 1a of the U.S. Environmental
Protection Agency’s (EPA) draft 2003 Update of Ambient Water Qua lity Criteria for C opper. EPA
will use these BLM data to derive adjusted aquatic life criteria for c opper. Many of the reported Table
1a acute copper toxicity data lack sufficient information on the chemistry of the dilution water to
generate BLM-derived critical accumulation values. This compendium contains data from the
primary authors of these articles. It also contains recommendations for the use of these data,
additional supporting documentation and/or computations, and recommendations for es timating
missing parameters.

### C -4 

# Estimation of Water Chemistry Parameters for Acute Copper Toxicity Tests

To prepare for the possibility of incorporating the Biotic Ligand Model (BLM) (Di Toro et al.

2001) into an updated copper aquatic life criteria document, the U.S. Environmental Protection
Agency (EPA) sought to generate a data table summarizing the acute toxicity of copper to
freshwater organisms that included the following parameters: alkalinity, dissolved organic carbon
(DOC), pH, and the major anions (Cl and SO 4 

## ) and cations (Ca, Mg, Na, K) of the test water.
Published literature was reviewed and appropriate information tabulated, but measurements for many
of the aforementioned parameters were not reported. To resolve the overwhelming number of
missing test water chemistry values in the database, certain authors were contacted for additional

information and to obtain additional measurements in waters where critical information was either
not measured or not reported. EPA also attempted to determine appropriate methods for es timating
test water chemistry in the absence of reported values. The information received from the authors
and recommended procedures for estimating missing parameters are the subject of this report.

1.0 Data Acquisition

The authors of several studies were contacted for additional information on the chemistry of
the water or methods used in their studies. If the primary or corresponding authors could not be
contacted, an attempt was made to contact secondary authors or personnel from the laboratories

where the studies had been conducted. In a few instances, this in itial effort failed to pr oduce the
desired information, and censored databases (U.S. Geological Survey’s [USGS] National Stream
Quality Acc ounting Network [NASQAN] and EPA’s STOrage and RETrieval [STORET] data
warehouse) were consulted to obtain the missing data. As a last resort, other available sources of
water compositional data (e.g., city drinking water treatment officials) were contacted.

The acquired data were scrutinized for representativeness and usefulness in estimating surrogate
values to complete the water quality information in the original studies. Summary tables and figures
generated from these data are included in the following pages, which serve as the basis for the
addition of values in the spreadsheets. Information used for the tabular and graphical summaries of
these data is included in separate appendices.

2.0 Technical Issues and Co rresponding Recommendations

2.1 Estimating Ion Concentrations

Develop a methodology for es timating Ca, Mg, Na, K, Cl, and SO 4 

## concentrations in
laboratory-reconstituted waters.

Recommendation: The best approach for estimating ion concentrations in standard
laboratory-reconstituted water i nvolves scaling default ion concentrations based on measured
hardness. The default ion concentrations can be computed from the concentrations of the salts
added. The use of calculated ion concentrations as input for the BLM applies only to recons tituted
water prepared following the standard recipes reported in guidance documents for conducting acute
bioassays with aquatic organisms (ASTM 2000; U.S. EPA 1993) (see Table 1). If s imilar salts are
added in different amounts, then the ion concentrations must be calculated using the recipe reported

### C -5 

## in the article. Otherwise, specific ion ratios, and more importantly ion concentrations, cannot be

calculated.

Table 1. Standard Reconstituted Water Composition and Target Water Quality

Characteristics

Water Type Reagent Added (mg/L) Final Water Quality

NaHCO 3 

### CaSO 4 

### C 2H 2 

### O MgSO 4 

### KCl pH a 

### Hardness a 

### Alkalinity b



### Very Soft 12.0 7.5 7.5 0.5 6.4-6.8 10-13 10-13

Soft 48.0 30.0 30.0 2.0 7.2-7.6 40-48 30-35

Mod. Hard 96.0 60.0 60.0 4.0 7.4-7.8 80-100 60-70

Hard 192.0 120.0 120.0 8.0 7.6-8.0 160-180 110-120

Very Hard 384.0 240.0 240.0 16.0 8.0-8.4 280-320 225-245

a 

### Approximate equilibrium pH after 24-hour aeration

b 

### Expressed as mg/L CaCO 3



## When standard laboratory-reconstituted water is cited as the dilution water, and no additional

measurements are reported, the recommended approach for estimating ion concentrations is to use
the ion concentrations calculated from the amount of salts added for the type of recons tituted water
reported in the article. For example, if the range of hardness of the reconstituted water is reported as
80-100 mg/L CaCO 3 

## , then the specific ion concentrations calculated from the standard recipe for
moderately hard reconstituted water s hould be used for BLM input (see Table 2 and example
calculation in Appendix D-2). The use of ion concentrations calculated from the standard recipes
assumes that salts were stored in a manner to prevent hydration and that technician errors in
weighing of salts, measurements of dilution water, and measurement of solution volumes were

minimal.

Alternatively, if the authors state that moderately hard water was prepared following one of the
standard recipes, and they measured the hardness of the water, then the calculated ion concentrations
should be adjusted to account for any difference from the mean of the expected range. For example,
if the mean measured hardness in a test water prepared using the recipe for moderately hard
reconstituted water was 78 mg/L CaCO 3 

## , the Ca:Mg ratio would be 0.700 for all recons tituted water
types, and the respective Ca and Mg concentrations could be calculated using the following equations:

Ca = (0.4008 × measured hardness)÷[1+(1÷Ca:Mg ratio)] Equation 1

Mg = (0.2431 × measured hardness)÷(1+Ca:Mg ratio) Equation 2

The remaining ion concentrations are each mu ltiplied by 0.92 ( quotient of 78 and 85 mg/L CaCO 3 

## , the latter of which is the expected hardness for moderately hard reconstituted water), as in Table 1.

### C -6 

## Table 3 provides ion concentrations predicted for a standard reconstituted water mix using the

hardness adjustment in accordance with the example a bove.

Note that this same rationale for scaling the default major anions and cations in reconstituted
water also applies to a variety of natural surface and well waters. Analysis of St. Louis River, MN,
water and Western Fish Toxicology Station (WFTS) well water indicated that a strong linear
relationship also exists between water hardness and the major anion (Cl, SO 4 

## ) and cation (Ca, Mg,
Na) concentrations in these water types (see Sections 2.6, 2.7, and 2.19). The strong relationships
are consistent with findings

Table 2. Calculated Ion Concentrations Based on the Standard Salts Added

Water Type (Nominal Hardness Range) Specific Ions a 

### (mg/L)

Ca:Mg b 

### Expected Hardness
(mg/L CaCO 3 

### ) c 

### Ca Mg Na K Cl SO 4



### Very Soft
(10-13 mg/L CaCO 3 

### ) 1.75 1.51 3.28 0.262 0.238 10.2 0.700 11

Soft
(40-48 mg/L CaCO 3 

### ) 6.99 6.06 13.1 1.05 0.951 40.7 0.700 42

Moderately Hard
(80-100 mg/L CaCO 3 

### ) 14.0 12.1 26.3 2.10 1.90 81.4 0.700 85

Hard
(160-180 mg/L CaCO 3 

### ) 27.9 24.2 52.5 4.20 3.80 163 0.700 170

Very Hard
(280-320 mg/L CaCO 3 

### ) 55.9 48.5 105 8.39 7.61 325 0.700 339

a 

### Ion concentrations were calculated from standard salt recipes (refer to Table 1 and example calculation for very soft
water in Appendix D-1).

b 

### Ratio equals quotient of (Ca÷40.08) and (Mg÷24.31), where 40.08 and 24.31 are the molecular weights of Ca and
Mg, respectively, in units of mg/mmol.

c 

### Hardness calculated according to the concentrations of Ca and Mg given here and the equation given in Appendix

D-1.



## Table 3. Adjusted Ion Concentrations for a Standard Reconstituted Water Mix Based on
Reported Hardness

Moderately Hard Reconstituted

Water Hardness
(mg/L CaCO 3 

### ) Specific Ions (mg/L)

Ca Mg Na K Cl SO 4



### Nominal 85 a 

### 14.0 12.1 26.3 2.10 1.90 81.4

Adjusted 78 12.9 11.2 24.2 2.10 1.75 74.9

a 

### Expected hardness based on the amount of salts added (from Table 1). Calcium and magnesium are calculated
using Equations 1 and 2. Other adjusted values (italic and bold) are a result of the product of the ratio of measured
hardness (78 mg/L) to expected hardness (85 mg/L) and nominal ion concentrations, e.g., the adjusted sodium ion
concentration for a standard laboratory reconstituted water mix based on a reported total hardness of 78 mg/L CaCO 3 

### is: 78÷85=0.92; 0.92*26.3=24.2.

### C -7 

## presented in an earlier comprehensive report by Erickson (1985). Note, however, that b ecause there
is generally poor correlation between K and water hardness in the various ambient surf ace and gr ound
water types (see Section 2.6), the value calculated for K should not be scaled according to hardness.

2.2 pH Adjustment with HCl

Schubauer-Berigan et al. (1993) adjusted pH using HCl but reported only nominal hardness and

alkalinity. The tests were conducted at the EPA Office of Research and Development, MidContinent Ecology Division, Duluth, MN, using a standard very hard recons tituted water mix. The
authors need to be contacted to obtain any additional water chemistry data they might have.

Recommendation: Alkalinity and hardness were not measured in the tests reported in

Schubauer-Berigan et al. (1993), and no add itional water chemistry data are available from the study
(Phil Monson, U.S. EPA-Duluth, personal communication). The HCl required to adjust the pH was
assumed to be added in amounts too small to significantly affect any of the other water qua lity
parameters (Gerald Ankley, U.S. EPA-Duluth, personal communication). Based on these remarks, we
believe ion concentrations for this particular study should be es timated using met hods ou tlined in

Section 2.1.

2.3 Estimation of DOC

How should DOC be es timated if only total organic car bon (TOC) was measured in the study?

Can DOC be estimated if no measurements of organic car bon were reported in the study?

Recommendation: As a general rule, TOC values can be used directly in place of DOC for

dechlorinated and de-ionized city tap water, well water, and oligotrophic lake water (e.g., Lake
Superior water). TOC values are not recommended in place of DOC for water from estuaries,
wetlands, or higher order streams unless data are included that indicate otherwise. Rather, the
proportion of organic carbon expected to be dissolved in surf ace waters s hould be es timated and used
to scale the measured TOC value. When possible, the DOC:TOC ratio for a surface water s hould be
obtained using the USGS NASQAN dataset. The NASQAN dataset can be r eached thr ough the USGS
Web site (water.usgs.gov/nasqan/data/finaldata.h tml). If a representative ratio for a particular body of
water cannot be determined, the ratio for the particular water type (lake or stream) should be
obtained from the final draft of the Ambient Water Quality Criteria Derivation Met hodology Human
Health Technical Support Document (U.S. EPA 1998a, Table 2.4.11). A summary of these data, by
State, is provided in Appendix D-2. In this appendix, TOC is operationally defined as the sum of
DOC and particulate organic carbon (POC). The national mean fraction of organic carbon is 86
percent for streams and 88 percent for lakes. The DOC:TOC ratio can be applied to lakes or streams
within a State to obtain an estimate of DOC from values reported for TOC.

Example :

Reference Water Body TOC (mg/L) DOC:TOC Estimated DOC (mg/L)

Lind et al. manuscript St. Louis R, MN 32 0.87 28

### C -8 

## For tests with reconstituted, city tap, or well water, default DOC values can be applied if the

author does not report a measured value. The recommended default TOC (DOC) value for laboratory
prepared reconstituted water is 0.5 mg car bon/L (note: some newer laboratory water systems can
achieve a TOC of less than 0.5 mg/L). For regular city tap and well water, a value of 1.6 mg carbon/L
can be assumed. The recommended default value for laboratory-prepared reconstituted water is based

on the arithmetic mean of recent measurements of DOC in recons tituted water prepared at two
Federal (U.S. EPA Cincinnati, OH, and USGS Yankton, SD) and two consulting (Commonwealth
Biomonitoring and GLEC) laboratories (range 0.1 to 1 mg/L). The recommended default value for
dechlorinated city tap and well water is based on the arithmetic mean of measurements of DOC in
source water from Lake Ontario (Environment Canada, Burlington, ON) and the New River, VA
(City of Blacksburg, VA), and well water from Oak Ridge National Laboratory (Oak Ridge, TN) and
EPA’s WFTS (Corvallis, OR). The DOC values in these waters ranged from 1.1 to 2.5 mg/L.

For tests conducted in surf ace waters, we do not recommend the use of a default DOC value
because of the large variab ility of DOC observed. Rather, a reliable database such as USGS NASQAN

(as described above) should be searched for DOC measurements. If a database such as NAS QAN is
consulted, only those DOC measurements closest to the time of the st udy should be considered as
surrogate values. In general, these DOC concentrations should not differ by more than a factor of

1.25. If DOC measurements for the surface water ca nnot be obtained from a reliable source, then the
toxicity test should not be included in Table 1 for BLM normalization.

2.4 DOC in Lake Superior Water

Lake Superior water has been used in a number of acute and chronic toxicity studies included in

the Aquatic Life Criteria for Copper (U.S. EPA 1998b). Dissolved organic matter (DOM) in Lake
Superior is assumed to be anywhere from 1 to 3 mg/L (Russ Erickson, U.S. EPA-Duluth, personal
communication; McGeer et al. 2000). This value is expected to be at least 90 percent of TOC (or 2
mg/L) (see Spehar and Fiandt 1986). A default value based on r ecent measurements is needed for
DOC in Lake Superior water.

Recommendation: Recent measurements of TOC in Lake Superior dilution water are in

Appendix D-3 (Greg Lien, U.S. EPA-Duluth, personal communication). The geometric mean
concentration of TOC in Lake Superior dilution water from multiple measurements is 1.27 mg/L.
Given the recommendation in Section 2.3, the recommended DOC for Lake Superior dilution water is
1.1 mg/L (1.27 mg/L × 0.88).

2.5 Applying Water Chemistry Data to Lake Superior Water

The ionic composition included in the Table 1 spreadsheet for Lake Superior water is based on

concentrations converted from values reported in Erickson et al. (1996b): Ca at 0.68 meq/L = 13.6
mg/L; Mg at 0.24 meq/L = 2.9 mg/L; Na at 0.065 meq/L = 1.5 mg/L; K at 0.015 meq/L = 0.59 mg/L;

SO 4 

## at 0.070 meq/L = 3.4 mg/L; Cl at 0.035 meq/L = 1.2 mg/L; and alkalinity at 0.85 meq/L = 43
mg/L. The concentrations for most of these parameters were also reported in Biesinger and
Christensen (1972) and approximate those listed above. Should the Erickson et al. (1996b) data be
applied to all Lake Superior studies, or is there a stronger rationale for applying the Biesinger and

Christensen (1972) data to the older studies?

### C -9 

## Recommendation: We recommend applying the mean of the Erickson et al. (1996b) citation

and Biesinger and Christensen (1972) water chemistry data to all Lake Superior studies prior to 1987,
when the results were initially reported. After 1987, we recommend use of the Erickson et al.
(1996b) water chemistry data alone (Table 4). For each test, Ca and Mg concentrations s hould be
estimated using Equations 1 and 2, the Ca:Mg ratios given below, and the measured hardness of the

test water (Section 2.1). Ions other than K should be scaled according to the measured test hardness,

also discussed in Section 2.1.

Table 4. Recommended Spreadsheet Addition for Lake Superior Dilution Water

Applied to: Hardness
(mg/L CaCO 3 

### ) Alkalinity
(mg/L CaCO 3 

### ) Specific Ions (mg/L)

Ca Mg Ca:Mg Na K Cl SO 4



### Pre-1987 a 

### 46 42 13.6 3.0 2.75 1.3 0.57 1.2 3.4

Post-1987 b 

### 46 43 13.6 2.9 2.84 1.5 0.59 1.2 3.4

a 

### Mean of the Erickson et al. (1996b) and Biesinger and Christensen (1972) water chemistry data

b 

### Erickson et al. (1996b) water chemistry data alone



## 2.6 Predicting Ionic Composition of WFTS Well Water

The following studies seem were conducted at EPA’s WFTS using well water: Andros and
Garton (1980), Chapman (1975, 1978), Chapman and Stevens (1978), Lorz and McPherson (1976),
Nebeker et al. (1984a, 1986a, b), and Seim et al. (1984). Among these studies, however, there is a
wide range of hardness values (20-100 mg/L), and the ionic composition of the water was not always
reported.

The large variation in WFTS well water hardness, and consequently, ionic composition, is due to
seasonal variability (Samuelson 1976). The TOC content of this water has been reported to be 1.1
mg/L (McCrady and Chapman 1979), of which 100 percent is expected to be dissolved. A general
strategy is needed to predict the ionic composition of WFTS well water based on measured water

hardness.

Recommendation: The well feeding the WFTS is susceptible to influx from ground water during rain events in late fall and winter (November through March or April). During this period the

water hardness can reach measured levels as high as 100 mg/L CaCO 3 

## . Over the remaining months
(particularly from July to November), hardness stabilizes at around 25 to 40 mg/L CaCO 3 

## , as do other
water quality parameters (Al Nebeker, U.S. EPA Corvallis, personal communication; Samuelson
1976). It is important to note that the high hardness reported for WFTS well water is sporadic, even

in the winter.

The recommended strategy for filling the existing gaps in data reported from studies using this

well water is to estimate the ion concentrations on the basis of their relationship to the total
hardness measured during a particular test. The acceptab ility of tests c onducted using WFTS water
depends on the range of hardness values reported, i.e., if the hardness varies widely over the course of
a particular test, then perhaps the test should not be used. Regression analyses were performed using
measured hardness and ion data for the WFTS well water reported in Samuelson (1976), April 1972

### C -10 

## to April 1974, and supplemented with additional data from Gary Chapman, personal communication
(only those data from May 1974 to April 1978; see Appendix D-4). These relationships and the
corresponding regression equations are presented in Figures 1 through 6 (found at the end of this
report). Major ion concentrations for WFTS well water were predicted using the regression equations
over a wide range of water hardness (10 to 80 mg/L CaCO 3 

## ) to determine the accuracy of the
procedure (Table 5). The error between predicted and measured ion concentrations is generally within
10 percent for all ions except K, where a default value of 0.7 mg/L was chosen for all hardness levels
(actual range is 0.1 to 1.1 mg/L, with the majority of data falling between 0.5 and 0.9 mg/L). The

correlation coefficient (R 2 

## ) for the relationship between K and water hardness in WFTS well water
was only 0.124. Note: BLM predictions of copper g ill accumulation and toxicity are relatively

insensitive to the concentration of K, so errors in its estimation s hould not appreciably affect model
predictions. The following regression equations were used to generate the example data provided in

Table 5:

[Ca] = 0.3085 + (measured hardness * 0.2738)
[Mg] = 0.5429 + (measured hardness * 0.0573)

[Na] = 3.3029 + (measured hardness * 0.0713)

[Cl] = 2.7842 + (measured hardness * 0.1278)

[SO 4 

## ] = -3.043 + (measured hardness * 0.2816)

Lorz and McPherson (1976) and the Seim et al. (1984) tests were not run in WFTS well water,

but in water from different wells along the W illamette River. Water chemistry appears to be less
variable for these wells (Harold Lorz and Wayne Seim, personal communication). The following
additional water chemistry information for the two well water types used in these studies was
provided by the respective authors in January 2001.

Many of the studies conducted by Chapman used reverse osmosis treatment to maintain a

blended water supply that was of essentially constant ion content throughout the tests. All the test
data from Chapman appear to be acceptable; the only test complicated by fluctuating hardness was
the 22-month chronic zinc test with sockeye salmon, and that test produced only a NOEC.

Table 5. Predicted Ion Concentrations in WFTS Well Water Based on Measured

Hardness

Total Hardness
(Mean Measured value)
mg/L CaCO 3 

### Predicted Ion Concentrations (mg/L)

Ca Mg Na Cl SO 4 

### Default a



### K

15.00 4.42 1.40 4.10 4.70 1.18 0.70

20.00 5.78 1.69 4.46 5.34 2.59 0.70

25.00 7.15 1.98 4.82 5.98 4.00 0.70

30.00 8.52 2.26 5.17 6.62 5.41 0.70

35.00 9.89 2.55 5.53 7.26 6.81 0.70

40.00 11.26 2.83 5.88 7.90 8.22 0.70

45.00 12.63 3.12 6.24 8.54 9.63 0.70

50.00 14.00 3.41 6.60 9.17 11.04 0.70

55.00 15.37 3.69 6.95 9.81 12.45 0.70

60.00 16.74 3.98 7.31 10.45 13.85 0.70

65.00 18.11 4.27 7.67 11.09 15.26 0.70

### C -11 70.00 19.47 4.55 8.02 11.73 16.67 0.70

75.00 20.84 4.84 8.38 12.37 18.08 0.70

80.00 22.21 5.13 8.74 13.01 19.49 0.70

a 

### Value not corrected. Assume default value of 0.70 mg/L.

Recommended Spreadsheet Addition for Oregon Well Water.

Applied to: Hardness
(mg/L

CaCO 3 

### ) Alkalinity
(mg/L

CaCO 3 

### ) pH DOC Specific Ions a 

### (mg/L)

Ca Mg Ca:Mg Na K Cl SO 4



### Lorz and

McPherson

1976 95 66 6.8-7.9 1.6 B 

### 19 12 1.0 7.6 1.0 7.0 12

Seim et al.

1984 120 126 7.7 1.6 B 

### 34 8.6 2.4 15 0.7 5.0 2.3

a 

### Specific ion values were obtained through personal communication with the primary authors; hardness, alkalinity,
and pH values are as reported in the article. The Ca:Mg ratios were calculated on the basis of data provided by
authors, then Ca and Mg values used were back-calculated on the basis of these ratios and the measured test
hardness (see Equations 1 and 2).

b 

### Suggested default value for untreated well water (see Section 2.3).



## 2.7 Data for Measurement of Blacksburg/New River Water

A substantial amount of acute copper toxicity data to various freshwater organisms is reported

using dechlorinated City of Blacksburg, VA, tap water. These include studies by Belanger et al.
(1989), Cairns et al. (1981), Hartwell et al. (1989), and Thompson et al. (1980). Hardness,
alkalinity, and pH values are reported for City of Blacksburg water in all of these studies, but the
ionic compositional data are not. This information is required to obtain BLM-normalized LC50s for

these data.

Recommendation: According to Don Cherry (personal communication), tests conducted at
Virginia Polytechnic Institute and State University used City of Blacksburg, VA, tap water, which is
drawn from the nearby New River. Don Cherry collected a sample of New River water for analysis
under Work Assignment 1-20. The results of the analysis are provided in Appendix D-5. The sample
was of untreated natural water prior to any treatment by the City of Blacksburg. Values for treated
New River water (city) were provided by Jerry Higgins, Water Superintendent, City of Blacksburg.
Table 6 summarizes the measured values for New River and City of Blacksburg dechlorinated tap

water.

Historically, hardness and alkalinity vary substantially in dechlorinated City of Blacksburg tap
water and in raw New River water (Table 6). Some of this difference may be attributed to seasonal
effects. For example, strong seasonal influence was observed in both well water (influenced by surface

water, i.e., WFTS well water; see Section 2.6) and a natural surface water (St. Louis River, MN; refer
ahead to Section 2.19). Previously, we plotted ion concentrations against hardness for each of these
two water types (Figures 1 through 6 and Appendix D-6). The relationships were good in almost all
cases (positive, R 2 

## = 0.5 to 0.9), and the resultant regression equations were used to scale ion
concentrations according to reported water hardness. Incomplete datasets, however, preclude the use

### C -12 

## of the same approach for City of Blacksburg tap and raw New River water. Instead, we recommend
using the ion and hardness values from the City of Blacksburg water sample and USGS NASQAN ion
data, respectively (Table 6), to generate surrogate ion values for the respective waters that were not
reported in the previous studies (indicated by the shaded area in Table 6). The operation is simply to
multiply ion concentrations for the “ acquired data” by the ratio of hardness values in City of
Blacksburg and NASQAN water and the corres ponding test waters as was done in Section 2.1. We used
the NASQAN ion data as the basis for scaling the raw New River water ion es timates b ecause NASQAN represents data collected over several representative years, including the years in the
timeframe in which the studies of interest were initiated and completed. The exception was with
DOC. We felt that the DOC value obtained from the sample of New River water collected in August
2000 would be more representative than the few values generated from NAS QAN (all pre- 1980).

2.8 Cu Concentrations and Alkalinity

The methods sections of both Belanger and Cherry (1990) and Belanger et al. (1989) state that

total and dissolved Cu were measured, but it is not clear whether the reported LC50s are based on
total or dissolved copper concentration. Also, in Belanger and Cherry (1990), pH was adjusted with
sodium hydroxide (NaOH) or nitric acid (HNO 3 

## ), but only nominal pHs were reported. Alkalinity and
hardness after pH adjustment were not reported. Can alkalinity be adjusted for these tests?

Recommendation: The concentration Cu in algae is reported on a total metal basis in

Belanger et al. (1989) and Belanger and Cherry (1990). The Cu in water is reported on an acidsoluble basis. The acid-soluble concentration of Cu in water was used to derive the LC50. For all
intents and purposes, acid-soluble Cu can be considered as dissolved Cu because the acidification of the
filtrate after filtration is probably sufficient to obtain most of the Cu associated with colloidal
material. Normally a digestion procedure is required to convert all Cu to the dissolved form. If the
sample had not been filtered, it would not have been acceptable because it could have been elevated
by dissolution of particulate copper.

The pH levels achieved in the batch culture pH tests in Belanger and Cherry (1990) were

reported as 6.15, 8.02, and 8.95. Given the proximity of these values to the desired target pH values
of 6, 8, and 9, respectively, it would appear that the researchers were able to closely approximate the
nominal pH levels, including those selected for the acute heavy metal tests (also pH 6, 8, and 9,
respectively). Assuming that the target pH values of 6, 8, and 9 were achieved in the acute tests,
adjustment with NaOH and HNO 3 

## would have affected alkalinity, but probably not hardness or the
major anion and cation concentrations, except possibly Na. The contribution to Na by the addition
of NaOH was probably small, so no further adjustment would be necessary.C-13Table 6. Comparison of Values for Untreated (Natural) and Treated (Dechlorinated City of Blacksburg, VA) New River WaterSourceWaterType pHTotalHardness(mg/L CaCO3)TotalAlkalinity(mg/L CaCO3)Specific Ions (mg/L)Ca:MgratioDOC(mg/L)Ca Mg Na K Cl SO4NO3Acquired DataCity of Blacksburg, VAaCity 8.5 44 39 - -9.3 -33 45- - 1.5Cherry 2000 (08/00)bNew R. 8.0-52 15 0.6 6.6 2.0 6.1 9.8 0.72NASQANcNew R. -61-15 5.8 3.4 1.6 4.0 13 0.8 1.6 5.4Values To Be Applied to Table 1 Toxicity TestsdBelanger et al. 1989City 7.74540 11 4.2 9.5 1.6 34 46 - 1.6 1.5Hartwell et al. 1989City 7.57243 18 6.8 15 1.6 54 74 - 1.6 1.5Cairns et al. 1981City 7.02627 6.4 2.4 5.5 1.6 19 26 - 1.6 1.5Thompson et al. 1980City 7.24028 9.9 3.8 8.5 1.6 30 41 - 1.6 1.5Belanger et al. 1989New R. 8.29470 23 8.8 5.2 1.6 6.2 20 - 1.6 2Belanger and Cherry 1990 New R. 6, 8, 9 9874 24 9.1 5.4 1.6 6.4 21 - 1.6 2aData provided by Gerard (Jerry) Higgins of Blacksburg-Christianburg VPI Water Authority, Blacksburg, VA. Values presented are from a grab samplecollected January 31, 2000. Organic carbon (originally measured and reported as TOC) is assumed to be 100 percent dissolved.bSample provided by Don Cherry, Virginia Polytechnic Institute and State University, Blacksburg, VA, and analyzed by Environmental Health Laboratories,South Bend, IN. Values presented are from a grab sample collected August 2000. The value for Mg of 0.6 mg/L appears to be a reporting error, and was notused for subsequent calculations of total hardness or scaling of ion values.cData obtained from USGS NASQAN database. Values presented are means of 213 samples, except for DOC, which is a mean of sevensamples, collected andanalyzed from January 1973 to August 1995.dShaded area indicates mean values estimated from previously (NASQAN) or recently measured (Cherry2000 or City of Blacksburg; nonadjusted) ion values.All values have been rounded to two significant figures. Shaded values were derivedaccording to text above using the approach outlined in Section 2.1.

### C -14 

## Using a nomograph found in Faust and Aly (1981), alkalinity at pH 6 should be approximately

33 percent of the alkalinity at pH 8, and alkalinity at pH 9 should be 5 percent higher than the
alkalinity at pH 8 (Table 7). Therefore, the values for alkalinity in Table 7 should be used for the
acute toxicity tests presented in Belanger and Cherry (1990) in this case. For other analyses,
different adjustment factors may be appropriate, based on other interpretations from the Faust and
Aly nomograph or other methods as well. Appropriate consideration should also be given to the test
system equilibration with the atmosphere.

Table 7. Estimated Alkalinity in Natural Surface Water Based on pH

Source Water Nominal pH Alkalinity (mg/L CaCO 3 

### )

New River 6 24.5

8.1 74.2 a



### 9 77.9

Clinch River 6 47.6

8.3 144 a



### 9 152

Amy Bayou 6 40.2

8.3 122 a



### 9 128

a 

### Indicates values reported in text.



## 2.9 Calculation of DOC and Humic Acid

What was the technical approach used to calculate DOC and percent humic acid (HA) for the

Winner (1985) toxicity tests?

Recommendation: At a nominal HA concentration of 0.0 mg/L in soft and medium hardness

test waters, the DOC is assumed to be that of the ultrapure laboratory water, which is estimated to be
0.3 mg/L (approximately one-half of the recommended default value for DOC in laboratory water;
see Section 2.3). At nominal HA concentrations of 0.15, 0.75, and 1.50 mg/L, the DOC is calculated
by dividing by a value of 2, based on the assumption in the BLM User’s Guide (Di Toro et al. 2000)
that the percent carbon in HA is 0.50 (see example below and Table 8). B ecause the water used to
obtain these HA concentrations was ultrapure laboratory water, 0.3 mg carbon/L was added; final

rounded values of 0.38, 0.68, and 1.1 are recommended.

Table 8. Estimates of Dissolved Organic Carbon and Percent Humic Acid for the Winner (1985) Toxicity Tests

Humic Acid Added (mg/L) a 

### Calculated DOC (mg/L) Calculated Percent Humic Acid

0 0

. 3 1 0

0.15 0.38 28

0.75 0.68 60

1.5 1.1 74

### C -15 a 

### As indicated in Table 3 of Winner (1985).



## 2.10 Alkalinity of Lake Superior Water

For the Lind et al. (manuscript) tests conducted in Lake Superior water (adjusted with CaSO 4 

## or MgSO 4 

## ), is there any way to estimate alkalinity values?

Recommendation: For tests conducted in Lake Superior water, assume an alkalinity of 42

mg/L CaCO 3 

## (see Section 2.5).

2.11 Availability of LC50s

The LC50s reported by Collyard et al. (1994) are shown graphically in publication. The LC50s

provided in Table 1 are interpolated from the figure. Are the actual measured LC50s available from

the authors?

Recommendation: The actual LC50s generated and presented graphically in Collyard et al.

(1994) have been archived at U.S. EPA-Duluth, as reported by Gerald Ankley (personal
communication, 3 November 2000). These values are not readily available in any other form. The
data are acceptable as is on the basis of recommendations in the Guidelines (Stephan et al. 1985).
Precedence for the use of values gleaned from graphical data is provided in the 2001 Update of
Ambient Water Quality Criteria for Cadmium (U.S. EPA 2001).

2.12 Cl and Na Concentrations

Cl and Na ion concentrations of the tap water used for testing in Rice and Harrison (1983) were

derived from the addition of 20 mg/L sodium chloride (NaCl). What are the specific concentrations
of the individual ions from the addition of the salt? What concentrations do you suggest using for K

and SO 4 

## in this water?

Recommendation: The Cl content of the tap dilution water used in Rice and Harrison (1983)

was reported as having been derived from the addition of 20 mg/L of NaCl. Assuming that the initial
Na and Cl concentrations in tap water were essentially zero, the concentrations of these ions can be
calculated in the following way:

The molecular weight of NaCl is 58.44 g/mol. The atomic weight of Na is 22.98 mg/L and the
atomic weight of Cl is 35.453 mg/L.

The concentration of Na is:

20 mg NaCl/L * 1 mmol NaCl/58.44 mg NaCl = 0.342 mmol NaCl/L.

0.342 mmol NaCl * 1 mmol Na/1 mmol NaCl * 22.98 mg Na/1 mmol Na

= 7.86 mg Na/L.

The concentration of Cl is:

20 mg NaCl/L × 1 mmol NaCl/58.44 mg NaCl = 0.342 mmol NaCl/L.

### C -16 

## 0.342 mmol NaCl × 1 mmol Na/1 mmol NaCl × 35.453 mg Cl/1 mmol Cl

= 12.12 mg Cl/L.

Given the potentially large dichotomy between the default ion concentrations and measured

hardness of the water used in this study, we recommend adjusting the default SO 4 

## concentration
according to measured hardness as in Section 2.1. We do not, however, recommend adjusting the
current default value of 1.0 mg/L for K.

2.13 Calculating DOC in Dilution Water

The dilution water used in the acute copper toxicity tests with cutthroat trout in Chakoumakos
et al. (1979) was a different mix of spring water and de-ionized water for each test. Ca and Mg
concentrations were measured and reported for each of the test waters used, but measurements of the
other ions were reported only for the undiluted spring water. Based on a percentage dilution, ions
other than Ca and Mg were estimated in the following way: hardness was measured in the spring water
and in each of the test waters; the pr oportion of spring water was calculated for each test using these
measured hardness values; this proportion was then mu ltiplied by the concentration of, for example,
Na in the spring water to get an estimated Na value for each test. TOC in the spring water was 3.3
mg/L. Should the same approach as that used to es timate the other ions be used to calculate DOC,
which was only measured in undiluted spring water?

Recommendation: The concentrations of the major cations and anions in the dilution water

used by Chakoumakos et al. (1979) were calculated based on the percent dilution of natural spring
water with de-ionized water. The same correction can be used to estimate DOC, with the following
assumptions. First, the TOC in spring water was 100 percent dissolved. Second, the DOC of deionized water was 0.5 mg/L. If these assumptions are acceptable, the DOCs for H/H, M/H, L/H, H/M,
M/M, L/M, H/L, M/L, and L/L would be 3.3, 1.5, 0.75, 3.3, 1.7, 0.94, 2.8, 1.5, and 0.87 mg/L,
respectively.

2.14 Ionic Composition of Chehalis River Water

The ionic composition of Chehalis River, WA, water is needed to fill in existing data gaps used
for BLM analysis of acute toxicity reported in Mudge et al. (1993). The publication states, “ Water
quality data collected during this bioassay program is similar to historical data for Chehalis River

(WPPSS 1982) and other Pacific NW streams (Samuelson 1976).” Are data from Samuelson (1976)
acceptable for use in approximating these ion concentrations? Furthermore, are there any dissolved
or ionic LC50s available other than those reported in the publication?

Recommendation: The following additional water chemistry information for the Chehalis

River dilution water used in the studies reported by Mudge et al. (1993) was provided by the author on

20 November 2000. These measurements were made on Chehalis River water at the time of testing.
A corresponding value for DOC was obtained from the NAS QAN dataset.

Recommended spreadsheet addition for Chehalis River dilution water

Applied to: DOC
(mg/L) Specific Ions (mg/L)

Ca Mg Ca:Mg Na K Cl SO 4

### C -17 Mudge et al. 1993 3.2 a 

### 7.1 2.4 1.8 5.1 0.65 4.5 (May)
4.2 (Jun)
3.1 (Sep) 4.0 (May)
3.5 (May-Jul)

2.3 (Sep)

a 

### Value from the USGS NASQAN dataset, 1980-1982, when the tests were conducted.



## 2.15 Chemistry of Water in Howarth and Sprague (1978)

What is the ionic composition and organic carbon content of test waters used in Howarth and

Sprague (1978)? The waters used for testing were various mixes of University of Guelph (Guelph,
ON, Canada) well water and de-ionized well water. The de-ionized well water was reported as “ having
retained its original chloride content (22 mg/l),” but the values for the other major anion and cation
concentrations were not reported. Furthermore, the equation provided for calculating alkalinity from
pH and hardness (supposedly accounting for 96.7 percent of the variability) appears unreliable. For
example, using the equation and a total water hardness of 364 mg/L CaCO 3 

## at pH 9, one obtains an
estimated alkalinity value of 341 mg/L CaCO 3 

## . In contrast, the measured alkalinity reported in the
text for this level of hardness and pH was 263 mg/L CaCO 3 

## .

Recommendation: The equation provided in the text of Howarth and Sprague (1978) for
calculating alkalinity appears unreliable. The calculated alkalinity does not approximate measured
alkalinity within a reasonable degree of accuracy. Values of hardness, pH, and alkalinity in Di xon and
Sprague (1981a), which used the same water source in their toxicity tests, give greater evidence of
this; i.e., using the measured value of hardness of 374 mg/L CaCO 3 

## and a pH of 7.75, the alkalinity
calculated with the equation is 98 mg/L CaCO 3 

## . This compares rather poorly with the measured
alkalinity of 223 mg/L CaCO 3 

## . Instead, alkalinity can be estimated using the nomograph from Faust
and Aly (1981) as in Section 2.8.

It is possible to apply the procedure used with the Chakoumakos et al. (1979) data here, i.e.,
using the ratio of hardness in full-strength well water and de-ionized well water to calculate the
dilution of the other major ion concentrations. However, no values are given for Na or K in
University of Guelph well water. This study is also complicated by the reverse-osmosis unit used to
create the de-ionized well water. In particular, the statement concerning the retention of the original
Cl concentration in the de-ionized well water implies an ionic exchange that would also require a
cation (to maintain charge balance). The cation involved is unknown. As discussed in a phone
conversation with John Sprague on 17 November 2000, and later that day with Scott Howarth
(Environment Canada), NaCl may have leached thr ough the RO unit. Assuming that Na and Cl

leached thr ough the unit in equivalent proportions, a value of 14 mg/L for Na can be back-calculated
from the reported Cl concentration of 22 mg/L.

Default DOC concentrations of 1.6 and 0.5 mg/L were assumed for the well water and deionized water used in the tests, respectively (see Section 2.3). The DOC concentrations were adjusted
for each particular test water hardness level based on the pr oportion of well water and de-ionized
water used to achieve the desired test hardness level. In the example provided in Table 9, the dilution
factor of 0.27, based on the ratio of the average hardness of well water (366 mg/L CaCO 3 

## ) versus the
average hardness of well plus de-ionized well water (100 mg/L CaCO 3 

## ), was applied to the starting

DOC concentrations to achieve an estimate of the DOC concentrations at 100 mg/L CaCO 3 

## ). Table

### C -18 

## 9 shows the results of similar adjustments made for the major anions and cations based on the data
reported in Howarth and Sprague (1978).

2.16 Default Values for Analyte Concentrations

What value should be used when a specific analyte is not detected at its designated detection

limit?

Recommendation: The use of half the detection limit (DL) is most appropriate when the

concentration of an analyte is not detected. One-half the DL will closely approximate a repl acement value for censored data in a log-normally distributed population that includes several measured values
(Berthouex and Brown 1994; Dolan and El-Shaarawi 1991). This way some of the “ nondetect”
samples will actually be c ounted as detected.

Table 9. Example Calculations to Estimate Water Chemistry of Tests Conducted at 100 mg/L CaCO 3 

## by Howarth and Sprague (1978) Using a Mixture of University of Guelph

Well Water and De-ionized Water

Parameter (units in mg/L) De-ionized water Well Water Example Calculations

for Mixture

Hardness 0 366 100
(i.e., 0.27 dilution factor)

Ca 0 77 (from Dixon & Sprague
1981) 21

Mg 0 43 (from Dixon & Sprague
1981) 12

Na 14 (assuming NaCl used for
the softening process) 14 (estimated from [Cl]) 14

K 0 2.4 (based on personal

communication from Dr. Patricia
Wright, Univ. of Guelph,
Guelph, ON) 0.66

Cl 22 (stated as not having
changed from the water
softening process) 22 22

SO 4 

### 0 129 35

DOC 0.5 (default value for deionized waters) 1.6 (default value for well
waters) 0.8

Alkalinity (calculated using ratios as in Section 2.8):

at pH 6 0 a 

### 81.5 22

at pH 7 0 a 

### 205 55

at pH 8 0 a 

### 250 N/A

at pH 9 0 a 

### 263 70

a 

### Alkalinity in de-ionized well water is assumed to be 0.0 mg/L.

### C -19 

## 2.17 Organic Carbon Content of Samples

Can any information be obtained on the organic carbon content of the spring water / City of

Cincinnati, OH, tap water mixes used in Brungs et al. (1973), Geckler et al. (1976), Horning and
Neiheisel (1979), Mount (1968), Mount and Stephan (1969), and Pickering et al. (1977)?

Recommendation: The water used for all tests was a mixture of spring-fed pond water

(originating at the Newtown Fish Farm) and carbon-f iltered, demineralized Cincinnati tap water. The
water was mixed to achieve the desired test hardness level and discharged to a large (several thousand
gallon) concrete reservoir that fed the test system. The detention time varied anywhere from 30 to
90 days, depending on the study, which was sufficient to allow the growth of phytoplankton and
zooplankton in moderate abundance. No add itional information regarding the TOC (DOC)
concentration or treatment of this water is available at this time. The recommended organic carbon
content of spring/city water mix is currently a conservative 1.6 mg/L, but could be as high as 2.5
mg/L, the highest DOC concentration recorded for a natural surface or well water used for studies
included in this report (see Section 2.3). Considering the long retention time, and the fact that the
natural water was spring-fed pond water, the more conservative DOC value of 2.5 mg/L is

recommended for this water.

2.18 Additional Water Chemistry Data Needed

Additional water chemistry data are needed for Bennett et al. (1995) and Richards and Be itinger

(1995). In the case of Richards and Beitinger 1995, only the ranges of measured pH, alkalinity, and
hardness across all tests were given.

Recommendation: Detailed pH, alkalinity, and hardness values were provided by both
Bennett et al. (1995) and Richards and Beitinger (1995) (Appendixes D-7 and D-9, respectively).
The studies performed by Bennett et al. were conducted using dechlorinated City of Denton, TX, tap
water (from Lake Roy Roberts). The author was not able to provide any additional data regarding the
ionic composition of this water; however, based on supplementary data, mean values of pH,
alkalinity, and temperature were 8.07 and 89.7 mg/L CaCO 3 

## and 21.4 C, respectively. Richards and
Beitinger’s studies were conducted using standard recons tituted (hard) water. To estimate the ionic
composition of this water, refer to recommendations provided in Section 2.1.

2.19 Estimating Data for Waters

Values for DOC, TSS, Ca, Mg, Na, K, SO 4 

## , and Cl are needed for the following natural waters:

Water Body Reference

American River, California – sand filtered Finlayson and Verrue 1982

Clinch River – 11 : m filtered Belanger et al. 1989

Belanger and Cherry 1990

Amy Bayou Belanger and Cherry 1990 Blaine Creek, Kentucky – 1.6 : m filtered Dobbs et al. 1994

S. Kawishiwi Lind et al. manuscript

St. Louis River Lind et al. manuscript

Lake One Lind et al. manuscript

### C -20 

## Colby Lake Lind et al. manuscript

Cloquet Lake Lind et al. manuscript

Greenwood Lake Lind et al. manuscript

Embarrass River Lind et al. manuscript

Green Duwamish River Buckley 1983

Chehalis River Mudge et al. 1993

Pinto Creek, AZ Lewis 1978

Naugatuck River Carlson et al. 1986

Recommendation: On the following pages are data (current and/or historical, presented as

arithmetic means) from selected natural waters that were retrieved from NASQAN, STORET, or a
secondary source (as indicated). As mentioned earlier (see Sections 2.6 and 2.7), given the reasonably
good correlation between most of the major anion and cations (except K) and water hardness in
natural surface and well waters, we recommend using the ion and hardness values retrieved from these
various sources to estimate the ion concentrations in the test water used in the previous studies. The
operation, again, is simply to multiply the ion concentrations listed below by the ratio of hardness
values presented below and the earlier test waters.

Note that additional data were not available for Blaine Creek, KY, or Pinto Creek, AZ, and

although additional data were obtained from the City of Sacramento, CA, regarding the American
River, the default DOC value (8.2 mg/L) for California streams may be artificially high on the basis
of reported values of DOC in the Sacramento River (1.2 mg C/L), of which the American River is a
tributary. Therefore, the data from Finlayson and Verrue (1982) have been relegated to “ other data.”
Likewise, Amy Bayou is a highly contaminated and dynamic system (Don Cherry, personal
communication), and BLM normalization is not recommended for these data. A large annual
variability in water quality also excludes the use of surrogate STORET data for the Embarrass River,
MN, for BLM analysis (Lind et al. manuscript).

American River, CA (Appendix C -9). Source: Ron Myers, City of Sacramento, CA, Water Quality Laboratory

Applied to: Hardness
(mg/L

CaCO 3 

### ) Alkalinity
(mg/L

CaCO 3 

### ) pH DOC Specific Ions (mg/L)

Ca Mg Ca:Mg Na K Cl SO 4



### Finlayson and

Verrue 1982 21 22 7.5 - a 

### 5.6 1.8 2.0 3.0 - 2.6 3.8

a 

### DOC and K data for the American River were not available.

Clinch River, VA (Appendix D-5): Source: Don Cherry, VA Poly. Inst. & State Univ., Blacksburg,VA

Applied to: Hardness
(mg/L

CaCO 3 

### ) Alkalinity
(mg/L

CaCO 3 

### ) pH DOC Specific Ions (mg/L)

Ca Mg Ca:Mg Na K Cl SO 4



### Belanger et al.
1989, and
Belanger and
Cherry 1990 150 150 8.3 2.3 42 11 2.3 12 2.4 9.2 19

### C -21 S. Kawishiwi River, MN (Appendix C -10). Source: STORET

Applied to: Hardness
(mg/L

CaCO 3 

### ) Alkalinity
(mg/L

CaCO 3 

### ) pH DOC Specific Ions (mg/L)

Ca Mg Ca:Mg Na K Cl SO 4



### Lind et al.
manuscript 24 18 6.6 - a 

### 5.6 2.4 1.5 1.3 0.5 1.0 4.9

a 

### DOC data for this river were not available. TOC measurements reported by Lind et al. (manuscript) should be
adjusted based on a mean DOC:TOC ratio (0.8721) in Minnesota streams (see Section 2.3 and Appendix D-2).

Lake One, MN (Appendix C -10). Source: STORET

Applied to: Hardness
(mg/L

CaCO 3 

### ) Alkalinity
(mg/L

CaCO 3 

### ) pH DOC Specific Ions (mg/L)

Ca Mg Ca:Mg Na K Cl SO 4



### Lind et al.
manuscript 10 15 6.7 - a 

### 2.8 0.7 1.8 0.1 0.3 0.2 4.2

a 

### DOC data for this lake were not available. TOC measurements reported by Lind et al. (manuscript) should be
adjusted based on a mean DOC:TOC ratio (0.9677) in Minnesota lakes (see Section 2.3 and Appendix D-2).

Colby Lake, MN (Appendix C -10). Source: STORET

Applied to: Hardness
(mg/L

CaCO 3 

### ) Alkalinity
(mg/L

CaCO 3 

### ) pH DOC Specific Ions (mg/L)

Ca Mg Ca:Mg Na K Cl SO 4



### Lind et al.
manuscript 56 33 7.1 - a 

### 13.3 5.4 1.6 4.0 1.4 7.3 23

a 

### DOC data for this lake were not available. TOC measurements reported by Lind et al. (manuscript) should be
adjusted based on a mean DOC:TOC ratio (0.9677) in Minnesota lakes (see Section 2.3 and Appendix D-2).

Cloquet Lake, MN (Appendix C -10). Source: STORET

Applied to: Hardness
(mg/L

CaCO 3 

### ) Alkalinity
(mg/L

CaCO 3 

### ) pH DOC Specific Ions (mg/L)

Ca Mg Ca:Mg Na K Cl SO 4



### Lind et al.
manuscript 27 21 7.2 - a 

### 6.9 2.3 1.4 1.9 b 

### 1.4 c 

### 1.2 5.6

a 

### DOC data for this lake were not available. TOC measurements reported by Lind et al. (manuscript) should be
adjusted based on a mean DOC:TOC ratio (0.9677) in Minnesota lakes (see Section 2.3 and Appendix D-2).

b 

### Na data for this lake were not available. The Na value given here is based on data for Colby Lake, MN, and was
scaled on the basis of hardness (see Section 2.1): Na = 4.0 mg Na/L * (27 mg/L CaCO 3 

### / 56 mg/L CaCO 3 

### ).

c 

### K data for this lake were not available. The K value given here is from data for Colby Lake, MN. This value was
not scaled on the basis of hardness (see discussion of K-hardness relationship in Sections 2.1 and 2.7).

### D-22 Greenwood Lake (Appendix C -10), MN. Source: STORET

Applied to: Hardness
(mg/L

CaCO 3 

### ) Alkalinity
(mg/L

CaCO 3 

### ) pH DOC Specific Ions (mg/L)

Ca Mg Ca:Mg Na K Cl SO 4



### Lind et al.
manuscript 17 11 6.4 - a 

### 4 1.8 2.4 0.2 b 

### 0.3 c 

### 1.7 7.6

a 

### DOC data for this lake were not available. TOC measurements reported by Lind et al. (manuscript) should be
adjusted based on a mean DOC:TOC ratio (0.9677) in Minnesota lakes (see Section 2.3 and Appendix D-2).

b 

### Na data for this lake were not available. The Na value given here is based on data for Lake One, MN, and was
scaled based on hardness: Na = 0.1 mg Na/L * (17 mg/L CaCO 3 

### / 10 mg/L CaCO 3 

### ).

c 

### K data for this lake were not available. The K value given here is from data for Lake One, MN. This value was
not scaled on the basis of hardness (see discussion of K-hardness relationship in Sections 2.1 and 2.7).

St. Louis River, MN (Appendix C -6). Source: NASQAN

Note: for the St. Louis River dataset (1973 to 1993), a question arose as to which data would be most representative
for estimating the ion concentrations in St. Louis River water for BLM analysis. In order to determine this, the
relationship between hardness and Na ion for all 20 years was plotted. Linear regression was used to fit the data.
Most data showed very high coefficient correlation (0.8-0.94). For each of these 20 regression lines, the slope and
intercept coefficients were plotted on separate graphs as functions of time (Figures 7 and 8). The following

conclusions were derived:

C A significant event occurred in 1976 and perhaps 1977 that affected the water balance of the St. Louis River. A
wastewater treatment plant was built, which substantially improved the water quality (Jesse Anderson, Minn.
Pollution Control Bd., personal communication).

C For the 1979-1993 period, hardness and ion concentrations did not change significantly as absolute values.
Therefore, general equations (which could be used to extrapolate water chemistry data till year 2000 and before
1979) can be obtained connecting hardness, alkalinity, pH, and the major ion concentrations.

C The exponential growth in the values between 1973 and 1979 shows that averaging values on seasonal and
annual basis is not appropriate. The constant values for the slopes and intercepts for 1979-1993 allow mean
monthly and annual interpretation of the data.

C The regression equations derived for 1977 alone are recommended to predict ion concentrations based on the
water hardness levels measured in the Lind et al. (manuscript). The equations derived for each ion are
provided in Appendix D-6 with the corresponding figures.

Green-Duwamish River, WA. Source: James Buckley

Applied to: Hardness
(mg/L

CaCO 3 

### ) Alkalinity
(mg/L

CaCO 3 

### ) pH DOC Specific Ions (mg/L)

Ca Mg Ca:Mg Na K Cl SO 4



### Buckley 1983 33 29 7.2 3.2 a 

### 8.9 2.8 2.0 7.5 1.2 7.0 6.3

a 

### Value given as TOC. DOC data for this river were not available. TOC measurements reported by Buckley et al.
(1983) should be adjusted on the basis of a mean DOC:TOC ratio (0.7803) in Washington streams (see Section 2.3
and Appendix C -2).

### C -23 Naugatuck River, WA. Source: STORET

Applied to: Hardness
(mg/L

CaCO 3 

### ) Alkalinity
(mg/L

CaCO 3 

### ) pH DOC Specific Ions (mg/L)

Ca Mg Ca:Mg Na K Cl SO 4



### Carlson et al.

1986 39 20 6.4 3.7 a 

### 9.9 3.3 1.9 9.9 2.3 - 22

a 

### Value given as TOC. DOC data for this river were not available. TOC measurements reported by Carlson et al.
(1986) should be adjusted on the basis of a mean DOC:TOC ratio (0.8711) in Connecticut streams (see Section 2.3
and Appendix C -2).

# Total Hardness (mg/L as CaCO3) Fi gure 1

. R e l a ti ons hi p b e t ween C a an d h ar d ness i n WFTS we ll wa t er

# Figure 2. Relationship between Mg and hardness in WFTS well water.

Total Hardness (mg/L as CaCO 3 

# )

# Figure 3. Relationship between Na and hardness in WFTS well water.



# Total Hardness (mg/L as CaCO3)

# Fi gure 4

. R e l a ti ons hi p b e t ween K an d h ar d ness i n WFTS we ll wa t er



# Total Hardness (mg/L as CaCO3)

# Figure 5. Relationship between Cl and hardness in WFTS well water.Cl (mg/L)



# Total Hardness (mg/L as CaCO 3 

# )

# Figure 6. Relationship between SO 4 

# and hardness in WFTS well water.SO4(mg/L)



# Total Hardness (mg/L as CaCO 3 

# )

### C -30 

# Figure 7. Slopes of the regression equations derived for Na concentration in St. Louis River, MN, water versus water

hardness from 1973 to 1993.

### C -31 

# Figure 8. Intercepts of the regression equations derived for Na concentration in St. Louis River, MN water versus water

hardness from 1973 to 1993.

### C -32 

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DC.

U.S. EPA. 2001. 2001 update of ambient water quality criteria for cadmium. EPA-822-R-01-001. Office of Water.
Washington, DC.

Winner, R.W. 1985. Bio accumulation and toxicity of c opper as affected by interactions between humic acid and
water hardness. Water Res. 19(4):449-455.

WPPSS (Washington Public Power Supply System). 1982. Supply system nuclear project No. 3. Environmental
Report - Operating License Stage Docket No. 50-508. Richland, WA.

### C-35 

# Appendix C -1. Calculations for Ionic Composition of Standard

Laboratory-Reconstituted Water

Molecular Weights Atomic Weights

NaHCO 3 

### = 84.03

CaSO 4 

### .2H 2 

### O = 172.12 MgSO 4 

### = 120.37

KCl = 74.55

SO 4 

### = 96.06 Na = 22.98

Ca = 40.08 Mg = 24.31

K = 39.10

Cl = 35.45

Example Calculation

[Na] in very soft water: 12 mg NaHCO 3 

### /L x 1 mmol NaHCO 3 

### /84.03 mg NaHCO 3 

### = 0.143 mmol NaHCO 3 

### /L.

0.143 mmol NaHCO 3 

### /L x (1 mmol Na/1 mmol NaHCO 3 

### ) x 22.98 mg Na/1 mmol Na = 3.3 mg Na/L.

[Ca] in very soft water: 7.5 mg CaSO 4 

### .2H2O/L x 1 mmol CaSO 4 

### .2H 2 

### O/172.12 mg CaSO 4 

### .2H 2 

### O = 0.044 mmol CaSO 4 

### .2H 2 

### O/L.

0.044 mmol CaSO 4 

### .2H 2 

### O/L x (1 mmol Ca/1 mmol CaSO 4 

### .2H 2 

### O) x 40.08 mg Ca/1 mmol Ca = 1.8 mg Ca/L.

[Mg] in very soft water: 7.5 mg MgSO 4 

### /L x 1 mmol MgSO 4 

### /120.37 mg MgSO 4 

### = 0.062 mmol MgSO 4 

### /L. 0.062 mmol MgSO 4 

### /L x (1 mmol Mg/1 mmol MgSO 4 

### ) x 24.31 mg Mg/1 mmol Mg = 1.5 mg Mg/L.

[K] in very soft water: 0.5 mg KCl/L x 1 mmol KCl/74.55 mg KCl = 0.0067 mmol KCl/L. 0.0067 mmol KCl/L x (1 mmol K/1 mmolKCl) x 39.102 mg K/1 mmol K = 0.26 mg K/L.

[Cl] in very soft water: 0.5 mg KCl/L x 1 mmol KCl/74.55 mg KCl = 0.0067 mmol KCl/L. 0.0067 mmol KCl/L x (1 mmol Cl/1 mmolKCl) x 35.453 mg Cl/1 mmol K = 0.24 mg Cl/L.

[SO 4 

### ] in very soft water: 7.5 mg CaSO 4 

### .2H 2 

### O/L x 1 mmol CaSO 4 

### .2H 2 

### O/172.12 mg CaSO 4 

### .2H 2 

### O = 0.044 mmol CaSO 4 

### .2H 2 

### O/L.

0.044 mmol CaSO 4 

### .2H 2 

### O/L x (1 mmol SO 4 

### /1 mmol CaSO 4 

### .2H 2 

### O) x 96.064 mg Ca/1 mmol Ca = 4.2 mg Ca/L.

[SO 4 

### ] in very soft water: 7.5 mg MgSO 4 

### /L x 1 mmol MgSO 4 

### /120.37 mg MgSO 4 

### = 0.062 mmol MgSO 4 

### /L. 0.062 mmol MgSO 4 

### /L x (1 mmol SO 4 

### /1 mmol MgSO 4 

### ) x 96.064 mg Mg/1 mmol Mg = 6.0 mg Mg/L.

Total SO 4 

### = 10.2 mg/L

Conversion Factors to calculate water hardness (as CaCO 3 

### ) from [Ca] and [Mg]:

[Ca] x 2.497 [Mg] x 4.116

### C-36



# Appendix C -2. Dissolved, Particulate, and Estimated Total Organic Carbon for Streams

and Lakes by State (as presented in EPA Document #822-B-98-005)

Streams Lakes

State POC DOC Est. TOC Est. DOC:TOC POC DOC Est. TOC Est. DOC:TOC

AK 0.54 4.6 5.14 89.49 0.53 6.4 6.93 92.35

AL 0.72 3.4 4.12 82.52

--- --- --- --- AR 0.8 7.2 8 90.00 0.4 2.7 3.1 87.10

AZ 0.71 5.2 5.91 87.99 0.52 4.2 4.72 88.98

CA 1.13 8.2 9.33 87.89 0.32 2.3 2.62 87.79

CO 1.29 8.6 9.89 86.96

--- --- --- --- CT 0.71 4.8 5.51 87.11

--- --- --- --- DC

--- --- --- --- --- --- --- --- DE* 0.7 7.1 7.8 91.03

--- --- --- --- FL^ 0.68 16.1 16.78 95.95 2.9 12.1 15 80.67

GA 0.67 4.3 4.97 86.52

--- --- --- --- HI 0.59 4 4.59 87.15

--- --- --- --- IA 1.79 11.6 13.39 86.63

--- --- --- --- ID 0.6 3.2 3.8 84.21

--- --- --- --- IL 1.77 6.8 8.57 79.35 0.12 4.7 4.82 97.51

IN 0.71 9.2 9.91 92.84

--- --- --- --- KS 1.75 5.2 6.95 74.82 1.53 4.5 6.03 74.63

KY 0.75 3.1 3.85 80.52

--- --- --- --- LA 1.52 6.9 8.42 81.95 0.65 5.6 6.25 89.60

MA 0.47 5.9 6.37 92.62

--- --- --- --- MD 1.66 3.7 5.36 69.03

--- --- --- --- ME 0.46 15.3 15.76 97.08

--- --- --- --- MI 0.58 6.3 6.88 91.57 0.32 2.7 3.02 89.40

MN 1.79 12.2 13.99 87.21 0.16 4.8 4.96 96.77

MO 0.56 4.2 4.76 88.24

--- --- --- --- MT 0.9 9.4 10.3 91.26 0.91 8.2 9.11 90.01

NC 1.14 11.5 12.64 90.98

--- --- --- --- ND 1.14 14.5 15.64 92.71 0.8 14.9 15.7 94.90

NE 1.84 6.8 8.64 78.70

--- --- --- --- NH 0.28 4.2 4.48 93.75

--- --- --- --- NJ 0.69 5.5 6.19 88.85 1.04 5 6.04 82.78

NM 1.43 6.3 7.73 81.50 0.51 5.2 5.71 91.07

NV 0.82 4.2 5.02 83.67

--- --- --- --- NY 1.4 4 5.4 74.07 0.46 2.4 2.86 83.92

OH 0.57 5 5.57 89.77 0.49 2.6 3.09 84.14

OK^ 1.27 7.7 8.97 85.84 1.72 15 16.72 89.71

OR*^ 1.14 2.1 3.24 64.81 0.64 4.4 5.04 87.30

PA 2.19 5.4 7.59 71.15 0.63 3.2 3.83 83.55

RI* 0.42 8.3 8.72 95.18

--- --- --- --- SC 0.7 5.7 6.4 89.06

--- --- --- --- SD 1.25 7.6 8.85 85.88

--- --- --- --- TN 0.67 2.3 2.97 77.44

--- --- --- --- TX 1.33 6.5 7.83 83.01 1.55 10.3 11.85 86.92

UT^ 1.38 8.9 10.28 86.58 0.5 2.4 2.9 82.76

VA 0.81 4.7 5.51 85.30

--- --- --- --- VT 0.31 4.5 4.81 93.56

--- --- --- --- WA 1.52 5.4 6.92 78.03 0.61 2.8 3.41 82.11

WI 1.03 9.2 10.23 89.93 0.16 4.1 4.26 96.24

WV 0.63 2.8 3.43 81.63

--- --- --- --- WY 1.07 8.2 9.27 88.46

--- --- --- ---

### Streams Lakes

State POC DOC Est. TOC Est. DOC:TOC POC DOC Est. TOC Est. DOC:TOC

C -37 Mean 85.71 Mean 87.84

Max 97.08 Max 97.51

Min 64.81 Min 74.63 * States where sample size was low for streams. ^ States where sample size was low for lakes.

### C-38 

# Appendix C -3. Mean TOC and DOC in Lake Superior Dilution Water

(data from Greg Lien, U.S. EPA-Duluth, MN)

Replicate Ambient (8/29/2000) pH 7.0 (8/30/2000) pH 6.2 (8/31/2000)

Filter Blank* -0.04 0.22 0.38

Pre-gill experiment TOC a 1.13 1.34 1.26

b 1.37 1.30 1.36

Mean 1.25 1.32 1.31

Post-gill experiment TOC a 1.20 1.24 1.18

b 1.27 1.46 1.10

Mean 1.24 1.35 1.14

Pre-gill experiment DOC a 1.96 1.51 1.34

b 1.52 1.28 0.99

Mean 1.74 1.40 1.17

Post-gill experiment DOC a 1.49 1.36 1.44

b 1.64 1.58 1.24

Mean 1.57 1.47 1.34 * Filter blank is ultra-pure Duluth-EPA laboratory water.

### C-39



# Appendix C -4. Measured Hardness and Major Ion and Cation Concentrations in WFTS Well Water from April 1972 to April 1978. Concentrations Given as Mg/L (data from Samuelson 1976 and Chapman, personal communication)

Month Total Hardness Ca Mg Na K SO 4 

### Cl

Mar-72 Apr-72 7.9 2 5 1.1 <10.0 8 May-72 22 5.8 1.4 4.4 0.5 <5.0 7

Jun-72 24 5.8 1.6 4.4 0.5 3 7

Jul-72 23 6.7 1.6 4.6 0.5 <1.0 8.3 Aug-72 23 6.5 1.7 4.7 0.5 <10.0 6.3 Sep-72 22 6 1.6 4.5 0.6 <10.0 4

Oct-72 22 6.7 1.9 4.7 0.6 5 5.5

Nov-72 23 6.2 1.6 4.2 0.6 3.7 5.3

Dec-72 23 6.2 1.5 4.2 0.5 3 4

Jan-73 52 15.3 3.5 7.1 0.7 7.8 12.4

Feb-73 33 7.7 2.1 5 0.5 5 5

Mar-73 30 8 2.1 5.3 0.7 5 6 Apr-73 31 8.9 2.3 5.4 0.7 5.3 8.8 May-73 28 8.3 2.4 5.8 0.7 3 8

Jun-73 28 8.4 2.2 5.8 0.7 4.8 7.5

Jul-73 26 7.4 1.9 5.8 0.8 <5.0 6.8 Aug-73 25 6.5 1.7 5.7 0.7 3.1 5.8 Sep-73 25 6.7 1.7 5.4 0.7 3.1 5.3

Oct-73 27 7 1.8 5.4 0.7 2.9 5.4

Nov-73 28 7.9 2.1 4.8 0.7 10 6.8

Dec-73 62 20.3 4.2 9 0.8 13 14

Jan-74 67 21.3 4.8 7 0.8 17.3 11.3

Feb-74 58 14.3 3.4 6.9 0.9 14.7 6.7

Mar-74 53 20.8 3.8 7.2 0.7 13 7 Apr-74 51 18.2 3.7 6.8 0.6 15.5 8.5 May-74 23 7.5 2.1 4.6 0.6 5 4.8

Jun-74 22 6 1.9 4.8 0.5 3 4.5

Jul-74 23 5.4 1.7 5 0.6 3.3 6.3 Aug-74 23 4.8 1.6 5 0.7 3 6 Sep-74 23 5.8 1.5 5.1 0.7 2.9 4.8

Oct-74 23 11 2 7.1 0.8 3.1 5

Nov-74 23 12 2.6 4.5 0.5 3.8 5.3

Dec-74 24 6.4 2.5 5.2 0.7 3.8 5

Jan-75 41 7.7 2.9 6.7 0.6 8 8

Feb-75 61 11.6 4.2 8.6 0.8 16 11.8

Mar-75 54 9.1 3.1 6.4 0.6 8 8 Apr-75 4.4 1.6 4.4 0.5 3 5 May-75 7.2 2 5 0.5 6 7

Jun-75 4.4 1.6 4.6 0.6 5 6

Jul-75 5.2 1.6 7 0.7 5 7 Aug-75 5.2 1.4 7 0.6 5 5 Sep-75 4.5 1.5 4.5 0.7 5 4

Oct-75 7.1 1.9 4.3 0.5 20 5

Nov-75 18 5.3 1.5 4.2 0.5 5 4

Dec-75

Jan-76

Feb-76 9.8 5 5.4 0.4 9 9

Mar-76 4.1 0.1 3 6 Apr-76 5.3 0.1 6 9

### Month Total Hardness Ca Mg Na K SO 4 

### Cl D

C

- 40 May-76 7.9 1.8 4.5 0.5 3 6

Jun-76 27 8.1 1.9 3.3 0.6 4 7

Jul-76 26 Aug-76 23 4.9 1.3 4.8 0.1 3 6 Sep-76 23 6.7 2.6 4.7 0.1

Oct-76 21 6.7 2.6 4.7 0.1

Nov-76 22 7.7 3 4.7 0.1 3

Dec-76 25.5 6.4 1.8 5 0.1 4 7

Jan-77 27.2 7.7 2.6 5.6 0.6 4 8

Feb-77 10.7 4.9 5.9 0.6 3 11

Mar-77 3 8 Apr-77 10.7 2.2 5.5 0.8 3 7
May-77 25 5 1.8 5 0.8 3 5

Jun-77 27 6.6 2 5.2 0.7 3 5

Jul-77 24 6.7 2 7.1 0.8 3 7 Aug-77 25 6.9 1.9 6.9 1 8 Sep-77 27 9.9 2.1 5.9 0.9 3 6

Oct-77 3

Nov-77 6.6 2.1 5.6 0.9 10 4.6

Dec-77 27 9.7 4.95 0.65 9 4.6

Jan-78 10.9 3.75 0.85 6 12

Feb-78 10.6 3.8 8.6 0.7 5 11

Mar-78 10.2 2.6 4.7 0.6 6 9 Apr-78 8.3 2.4 0.7 5 9.55

### Date pH Hardness Alkalinity Ca Mg Na K Cl SO 4 

### NO 3 

### DOC D

C -41 19790329 7.6 80 63 19 8 8.4 2.3 7.8 13

19790430 7.6 37 29 8.7 3.7 2.2 1.3 2.8 8.9 20

19790611 7.2 47 34 11 4.8 3.1 0.8 2.8 9.4

19790723 7.6 73 55 17 7.3 3.9 0.9 3.7 8.9 30

19790827 7.2

19791015 8.1 74 54 16 8.2 5 1.1 3.9 13 0.01 12

19791126 7.8 61 52 14 6.3 3.8 0.9 3.6 11 0.37

19800121 7.6 60 53 14 6 3.8 0.9 3.2 9.9 0.15

19800219 7.4 63 51 15 6.2 3.9 0.8 2.9 9.2 0.19 17

19800331 8.4 68 64 16 6.9 4.2 1.1 3.5 9.2 0.3

19800602 8.3 84 72 19 8.8 6.4 1.2 5 15 0.01 21

19800630 8.3 93 68 21 9.9 7.9 1.4 6.7 24 0.02

19800804 8.1 130 110 28 14 10 1.9 11 24 0.01 13

19800902 7.8 110 82 24 11 7.2 1.7 7.6 18 0.01

19800929 7.6 73 54 16 8.1 5.7 1.4 5.8 14 0.12

19801103 7 82 58 18 8.9 5.6 1.3 6.9 18 0.19 23

19801208 67 50 15 7.2 4.6 1 4.1 11 0.19

19810105 7.6 70 55 16 7.2 4.2 1.1 4.1 13 0.23

19810209 7.5 68 58 16 6.9 4.9 1 3.5 8.1 0.27 14

19810309 7.7 61 57 14 6.2 5.2 1.8 5.1 8.6 0.36

19810504 7.3 42 40 9.6 4.3 3.7 1.2 3.6 9.6 0.18 21

19810706 7.4 51 39 12 5 3.5 1.2 3.2 7.5 0.14 10

19810908 7.9 73 64 16 8 4.2 0.8 4.2 8.3 0.11

19811020 7.6 51 37 12 5.2 4.3 1.2 4.2 8.9 0.31

19820113 62 52 14 6.5 4 0.9 3.7 9.3 0.24

19820309 7.4 66 58 15 7 5.3 1 3.8 11 0.36

19820420 7.2 32 25 7.5 3.3 2.1 1.3 2.3 6 0.19

19820621 7.9 61 55 14 6.4 4.3 1.1 4 10 0.1

19820809 7.4 66 54 15 6.9 3.9 0.6 3.5 9 0.25

19821004 8 73 63 15 8.7 4.9 1 4.7 13 0.11

19821207 7.3 55 43 12 6.1 4.2 0.8 3.3 16 0.24

19830131 6.9 62 50 14 6.5 4.1 0.8 3.5 15 0.36

19830328 7.5 68 56 15 7.3 4.5 1.2 4.1 15 0.35

19830523 8.2 68 53 15 7.5 4 1.3 0.8 23 0.12

19830718 7.6 67 53 15 7.2 3.7 1.3 3.7 22 0.15

19831031 7.7 64 48 14 7 3.9 1.2 3.5 24 0.12

19840109 7.4 57 50 13 6 3.6 0.9 3.4 13 0.23

19840306 7.1 66 57 15 7 4.4 0.9 5.2 8.7 0.31

19840424 7.2 51 39 11 5.6 3.1 1.4 3.2 14 0.12

19840619 9.5 52 39 12 5.3 2.9 0.8 3.6 10 0.13

19840822 6.4 70 58 15 7.9 4.7 1 3.8 17 0.1

19841009 7.6 73 16 7.9 4.6 1 3.7 15 0.1

19841120 7.1 64 14 7.1 3.9 0.9 3.7 14 0.24

19850211 7 69 15 7.7 4.6 1.1 4 11 0.27

19850325 7.3 61 13 7 5.6 2.5 6.6 16 0.31

19850506 7.4 55 12 6 3.6 1.7 4.2 14 0.15

19850730 7.6 62 14 6.6 3.2 0.9 4 9.8 0.1

19851021 7.5 58 12 6.8 3.7 1.1 0.2 12 0.13

### C-42



# Appendix C -6. Water Composition of St. Louis River, MN, from USGS NASQAN and Select Relationships to Water Hardness

Date pH Hardness Alkalinity Ca Mg Na K Cl SO 4 

### NO 3 

### DOC

19730222 6.8 68 53 17 6.3 11 1.6 14 14 0.19

19730503 7.1 58 46 14 5.5 6.6 1.1 9.5 13 0.17

19730816 6.9 70 51 17 6.6 7.6 1.2 9 20 0.01

19731128 7 65 48 16 6.1 7.5 1.3 8.8 14

19740221 7 64 48 16 5.8 8.9 1.3 12 14

19740516 6.9 45 32 11 4.3 3.5 1.2 3.8 11

19740919 88 60 21 8.6 12 1.8 17 23

19741030 7.3 83 62 23 6.3 13 1.3 16 23

19741209 7.4 86 62 22 7.6 12 1.6 15 18

19750121 7.3 74 66 18 7 10 1.1 12 13

19750303 7.3 74 68 17 7.6 10 1.7 11 12

19750407 7.2 95 80 22 9.7 11 2 14 16

19750527 7.5 63 50 15 6.1 8.5 1.5 9.2 12

19750708 9.2 58 43 14 5.7 3.2 1 3.4 10

19750818 7.2 73 56 18 6.9 12 1.3 16 16

19750929 7.4 90 72 23 8 12 1.5 13 20

19751110 7.1 90 63 22 8.4 12 1.7 15 24

19751216 7.6 87 61 22 7.8 14 1.6 16 28

19760209 7.5 72 59 18 6.6 13 1.6 13 18

19760322 7.7 78 65 19 7.4 12 1.4 11 17

19760503 7.6 59 43 14 5.8 7.9 1.3 8.6 15

19760614 7.5 94 75 22 9.4 16 1.9 20 20

19760726 7.4 93 80 22 9.3 21 1.9 25 24

19760908 7.5 82 78 18 9.1 17 2.5 9.3 26

19761019 7.5 83 72 20 8.1 21 1.6 24 21

19761129 7.4 95 74 22 9.7 25 1.8 32 24

19770110 7.3 85 88 20 8.4 17 1.5 15 19

19770214 8.2 82 73 20 7.8 18 1.7 26 17

19770404 7.3 87 67 21 8.5 20 2.4 28 24

19770516 7.3 120 98 29 11 30 2.8 26 36

19770628 7.8 100 75 24 9.9 13 2 16 23

19770808 7.4 110 90 26 10 27 2.2 32 28

19770919 7.4 73 44 17 7.3 6.6 1.7 8.9 17

19771031 7.6 64 47 15 6.5 7.9 1.3 9.7 22 37

19771212 7.5 65 50 15 6.8 6.3 1.2 7.1 16

19780123 7.3 71 52 17 6.9 12 1.5 9.4 18

19780306 7.2 67 48 16 6.5 8.8 1.2 17 16 32

19780417 7.5 43 28 10 4.3 4.2 1.8 5.7 15

19780530 7.9 64 54 15 6.4 5.7 1.5 7.1 14 33

19780710 7.4 53 44 13 5.1 4.3 1.3 5.3 8.9

19780821 8.4 60 42 15 5.5 5.3 1.5 6.5 12 36

19781002 7.7 71 57 17 6.9 8.2 1.1 9.6 15 24

19781115 7.4 68 52 16 6.8 11 1.1 10 12

19781218 7.4 68 55 16 6.9 11 1 9.2 14

19790205 7.4 63 57 15 6.3 3 34 .4 1 3.1 8 12

### Date pH Hardness Alkalinity Ca Mg Na K Cl SO 4 

### NO 3 

### DOC D

C -43 19790329 7.6 80 63 19 8 8.4 2.3 7.8 13

19790430 7.6 37 29 8.7 3.7 2.2 1.3 2.8 8.9 20

19790611 7.2 47 34 11 4.8 3.1 0.8 2.8 9.4

19790723 7.6 73 55 17 7.3 3.9 0.9 3.7 8.9 30

19790827 7.2

19791015 8.1 74 54 16 8.2 5 1.1 3.9 13 0.01 12

19791126 7.8 61 52 14 6.3 3.8 0.9 3.6 11 0.37

19800121 7.6 60 53 14 6 3.8 0.9 3.2 9.9 0.15

19800219 7.4 63 51 15 6.2 3.9 0.8 2.9 9.2 0.19 17

19800331 8.4 68 64 16 6.9 4.2 1.1 3.5 9.2 0.3

19800602 8.3 84 72 19 8.8 6.4 1.2 5 15 0.01 21

19800630 8.3 93 68 21 9.9 7.9 1.4 6.7 24 0.02

19800804 8.1 130 110 28 14 10 1.9 11 24 0.01 13

19800902 7.8 110 82 24 11 7.2 1.7 7.6 18 0.01

19800929 7.6 73 54 16 8.1 5.7 1.4 5.8 14 0.12

19801103 7 82 58 18 8.9 5.6 1.3 6.9 18 0.19 23

19801208 67 50 15 7.2 4.6 1 4.1 11 0.19

19810105 7.6 70 55 16 7.2 4.2 1.1 4.1 13 0.23

19810209 7.5 68 58 16 6.9 4.9 1 3.5 8.1 0.27 14

19810309 7.7 61 57 14 6.2 5.2 1.8 5.1 8.6 0.36

19810504 7.3 42 40 9.6 4.3 3.7 1.2 3.6 9.6 0.18 21

19810706 7.4 51 39 12 5 3.5 1.2 3.2 7.5 0.14 10

19810908 7.9 73 64 16 8 4.2 0.8 4.2 8.3 0.11

19811020 7.6 51 37 12 5.2 4.3 1.2 4.2 8.9 0.31

19820113 62 52 14 6.5 4 0.9 3.7 9.3 0.24

19820309 7.4 66 58 15 7 5.3 1 3.8 11 0.36

19820420 7.2 32 25 7.5 3.3 2.1 1.3 2.3 6 0.19

19820621 7.9 61 55 14 6.4 4.3 1.1 4 10 0.1

19820809 7.4 66 54 15 6.9 3.9 0.6 3.5 9 0.25

19821004 8 73 63 15 8.7 4.9 1 4.7 13 0.11

19821207 7.3 55 43 12 6.1 4.2 0.8 3.3 16 0.24

19830131 6.9 62 50 14 6.5 4.1 0.8 3.5 15 0.36

19830328 7.5 68 56 15 7.3 4.5 1.2 4.1 15 0.35

19830523 8.2 68 53 15 7.5 4 1.3 0.8 23 0.12

19830718 7.6 67 53 15 7.2 3.7 1.3 3.7 22 0.15

19831031 7.7 64 48 14 7 3.9 1.2 3.5 24 0.12

19840109 7.4 57 50 13 6 3.6 0.9 3.4 13 0.23

19840306 7.1 66 57 15 7 4.4 0.9 5.2 8.7 0.31

19840424 7.2 51 39 11 5.6 3.1 1.4 3.2 14 0.12

19840619 9.5 52 39 12 5.3 2.9 0.8 3.6 10 0.13

19840822 6.4 70 58 15 7.9 4.7 1 3.8 17 0.1

19841009 7.6 73 16 7.9 4.6 1 3.7 15 0.1

19841120 7.1 64 14 7.1 3.9 0.9 3.7 14 0.24

19850211 7 69 15 7.7 4.6 1.1 4 11 0.27

19850325 7.3 61 13 7 5.6 2.5 6.6 16 0.31

19850506 7.4 55 12 6 3.6 1.7 4.2 14 0.15

19850730 7.6 62 14 6.6 3.2 0.9 4 9.8 0.1

19851021 7.5 58 12 6.8 3.7 1.1 0.2 12 0.13

### Date pH Hardness Alkalinity Ca Mg Na K Cl SO 4 

### NO 3 

### DOC D

C -44 19851203 7.4 73 16 8 4 1 4.2 18 0.16

19860303 7.4 66 15 7 4 1 3.4 10 0.24

19860407 7.3 0.19

19860602 7.5 58 13 6.3 3.5 1 2.8 15 0.1

19860818 7.9 74 15 8.9 4.6 1.2 3.7 24 0.1

19861112 7.5 55 12 6 3.4 1.4 3.8 19 0.27

19861210 7.3 70 57 13 9 5 1 4.8 21 0.16

19870218 7 66 15 6.8 3.7 0.9 3.1 12 0.24

19870518 8 83 18 9.3 5.8 1.2 5 10 0.1

19870622 7.8 75 16 8.5 6.2 1.1 5.2 19 0.1

19870721 7.6 51 12 5.2 2.8 1.3 3.1 15 0.1

19871028 8 82 17 9.6 6.8 1.4 1.3 19 0.1

19871208 7.9 69 15 7.7 5.3 1.4 4.8 17 0.1

19880119 7.4 73 16 8 5.1 1 3.6 15 0.15

19880223 7.4 85 19 9.2 6.5 8.5 5.1 16 0.2

19880412 7.4 42 9.2 4.7 3 2.8 5 20 0.25

19880907 7.1 70 15 8 5.3 1.5 6.1 18 0.15

19881031 7.6 100 21 12 9 1.9 7.8 27 0.1

19881130 7.6 78 17 8.6 5.5 1.3 5.5 19 0.19

19890221 7.1 77 17 8.4 6.3 1.3 4.4 17 0.25

19890410 7.2 48 11 5 4.9 1.8 8.1 8 0.37

19890626 7.4 63 14 6.8 4.6 1.1 5 12 0.15

19890814 8.1 95 20 11 9.1 1.5 8.9 18 0.1

19891101 8.1 110 20 15 7.8 1.9 6.3 31 0.1

19891218 7.5 88 17 11 6.1 1.4 5 22 0.16

19900123 7.3 100 18 14 7.2 1.7 5.2 28 0.23

19900416 7.5 62 13 7.2 5.1 1.9 5.4 14 0.2

19900716 7.7 70 15 8 5.7 1.3 5.4 11 0.2

19900820 8.1 95 20 11 7.8 1.5 7.9 20 0.1

19901009 7.3 81 18 8.7 5.4 1.5 5.7 13 0.1

19910102 7.4 83 19 8.7 5.3 1.4 5 12 0.2

19910212 7.1 80 18 8.5 6.8 1.3 3.9 11 0.2

19910502 6.7 56 13 5.8 4 1 3.7 7.9 0.1

19910610 7.3 64 15 6.5 4 0.7 4.1 6.9 0.12

19910731 7.8 55 13 5.4 2.5 1 2.6 3.8 0.05

19910801 7.3

19911003 7.8 67 15 7.1 4.4 1 4.4 9.6 0.068

19911204 7.4 61 13 6.9 4.8 1 3.5 7 0.18

19920113 7.9 67 15 7.2 4.3 1.1 3.2 9.3 0.21

19920413 7.7 30 7.8 2.5 2.5 0.3 2.4 4.8 0.16

19920722 7.6 71 16 7.5 4.8 0.9 2.1 9.6 0.11

19921026 8.2 86 18 10 5.3 1.2 5.4 14

19921216 7.6 89 19 10 6 1.2 5.6 13 0.25

19930201 7.2 83 18 9.1 7.3 1.2 7.3 12 0.28

19930426 7.7 66 15 6.8 4.1 1.2 4.9 9.5 0.092

19930722 7.5 64 15 6.5 4 0.2 3.9 7.7 0.079

19931201 7.7 80 17 9 4.8 1 4 11 0.16

### Date pH Hardness Alkalinity Ca Mg Na K Cl SO 4 

### NO 3 

### DOC D

C -45 19940216 7.3

19940511 7.7 51 11 5.6 3.7 1.1 3.4 9.4 0.076

MIN 6.4 30 25 7.5 2.5 2.1 0.2 0.2 3.8 0.01 10

MAX 9.5 130 110 29 15 30 8.5 32 36 0.37 37

MEAN 7.52 71.11 56.94 16.16 7.46 7.09 1.37 7.39 15.04 0.17 22.19

### C-46

### C-47

### C-48 

# Appendix C -7. Supplementary Data for Bennett et al. (1995)

Tank Dose

(μg Cu/L) Conductivity

(μmho/cm) pH Oxygen (mg/L) Temp

( o 

### C) Alkalinity

(as mg

CaCO 3 

### /L) Hardness

(as mg

CaCO 3 

### /L)

0 hours 7/9/92

a 897 325 8.62 7.5 21 100 96

b 897 300 8.6 7.6 21 100 96

c 897 320 8.6 7.6 21 80 96

d 607 320 8.62 7.7 21 80 96

e 607 370 8.62 7.6 21 80 96

f 607 328 8.64 7.6 21 80 96

g 93 310 8.64 7.6 21 80 96

h 93 370 8.69 7.5 21 80 96

I 93 310 8.6 7.6 21 80 96
j 505 310 8.62 7.7 21 100 96

k 505 310 8.65 7.7 21 80 96

l 505 320 8.69 7.7 21 80 96

m 319 320 8.69 7.7 21 80 96

n 319 330 8.68 7.7 21 80 96

o 319 320 8.67 7.7 21 80 96

p 0 310 8.62 7.5 21 80 96

q 0 320 8.63 7.6 21 80 96

r 0 320 8.6 7.7 21 80 96

24 hours 7/10/92

a 897 300 7.78 8.5 21.5 60 104

b 897 305 7.64 8.4 22 80 100

c 897 305 7.68 8.5 22 90 100

d 607 300 7.7 8.4 21.5 90 100

e 607 305 7.65 8.4 21.5 80 100

f 607 305 7.75 8.4 21.5 80 100

g 93 300 7.77 9.1 22 80 100

h 93 295 7.76 9.2 21.5 80 108

I 93 295 7.76 9 21.5 85 100
j 505 300 7.73 8.8 22 90 84

k 505 300 7.71 8.8 21.5 80 100

l 505 300 7.73 8.7 21.5 80 100

m 319 300 7.74 9.1 21.5 80 100

n 319 300 7.52 8.5 22 80 100

o 319 310 7.79 8.7 22.5 80 100

p 0 305 7.79 9.1 22 80 100

q 0 305 7.7 9.1 22 80 104

r 0 300 7.71 9.1 22 80 104

48 hours 7/11/92

a 897 * * * * * *

b 897 * * * * * *

c 897 320 8.1 7.2 21.5 100 96

d 607 315 7.91 6.9 21.5 100 96

e 607 310 7.84 6.8 21.5 100 100

f 607 315 8 7 21.5 100 104

g 93 300 8.19 7.7 21.5 100 100

### Tank Dose (μg Cu/L) Conductivity

(μmho/cm) pH Oxygen (mg/L) Temp

( o 

### C) Alkalinity

(as mg

CaCO 3 

### /L) Hardness

(as mg

CaCO 3 

### /L) D

C -49 h 93 300 8.13 7.7 21 100 100

I 93 300 8.16 7.6 21 100 104
j 505 310 8.1 7.5 21 80 100

k 505 310 8.12 7.4 21 100 100

l 505 310 8.13 7.4 21 80 100

m 319 310 8.12 7.4 21 100 100

n 319 310 7.8 6.4# 21.5 100 100

o 319 310 8.18 7.3 22 100 96

p 0 300 8.16 8 21.5 80 100

q 0 300 8.1 7.9 21.5 80 104

r 0 300 8.21 8 21.5 100 100

72 hours 7/12/92

a 897 * * * * * *

b 897 * * * * * *

c 897 * * * * * *

d 607 310 8.02 8.9 21.5 100 100

e 607 315 8.04 8.8 21.5 100 100

f 607 315 8.02 8.7 21.5 80 100

g 93 310 7.92 9.1 21.5 100 104

h 93 305 7.91 9.1 21 100 100

I 93 310 7.91 9 21 80 106
j 505 315 7.97 8.9 21.5 100 104

k 505 310 7.96 8.9 21 100 100

l 505 310 7.96 9 21 80 104

m 319 310 7.91 9 21 100 100

n 319 310 7.97 9 21 80 100

o 319 320 7.99 8.8 22 100 104

p 0 300 7.86 9.3 21.5 100 104

q 0 300 7.81 9.1 21.5 80 100

r 0 305 7.93 9.3 21.5 80 100

96 hours 7/13/92

a 897 * * * * * *

b 897 * * * * * *

c 897 * * * * * *

d 607 320 8.03 7.3 21.5 100 104

e 607 320 8.07 7.3 21.5 100 100

f 607 325 8.02 7.2 21.5 100 104

g 93 325 7.95 7.1 21.5 120 104

h 93 315 8.03 7.5 21 100 100

I 93 310 8.02 7.4 21 100 100
j 505 320 8.06 7.4 21.5 80 100

k 505 320 8.05 7.4 21 120 100

l 505 320 8.03 7.3 21 100 104

m 319 315 8.05 7.5 21 100 104

n 319 320 8.06 7.4 21 100 100

o 319 330 8.08 7.3 22 100 104

### Tank Dose (μg Cu/L) Conductivity

(μmho/cm) pH Oxygen (mg/L) Temp

( o 

### C) Alkalinity

(as mg

CaCO 3 

### /L) Hardness

(as mg

CaCO 3 

### /L) D

D -50 p 0 330 7.78 8.1 21.5 80 96

q 0 325 7.75 7.9 21.5 80 104

r 0 330 7.86 8.1 21.5 80 100
* All fish dead, no water quality measured.

# Air stone had fallen out of tank.

### C-51 

# Appendix C -8. Supplementary Data for Richards and Beitinger (1995)

Acclimation
Temperature 5°C 12°C 22°C 32°C

Replicate 1 2 1 2 1 2 1 2

Sample size 30 36 30 36 36 30 33 29

pH 8.2-8.3 7.8-8.2 8.4-8.5 8.2-8.4 8.3-8.4 8.1-8.5 8.4-8.5 8.4-8.5

Hardness (mg/l CaCO 3 

### ) 164-180 152-166 152-168 148-170 164-174 162-172 164-168 162-172

Alkalinity (mg/l CaCO 3 

### ) 125-140 130-140 130-140 130-140 140-145 140-145 135-140 135-145

Weights of minnows (g) 0.62-

3.23 0.42-2.64 0.56-2.38 0.30-1.93 0.66-

1.15 0.13-

1.55 0.26-

1.36 0.23-

1.32

Lengths of
minnows (cm) 3.3-5.5 3.2-5.2 3.2-4.9 2.8-5.1 1.9-4.3 2.4-4.6 3.0-4.8 3.3-4.8

### C-52



# Appendix C -9. Data for the American River, CA, for July 1978 Through December 1980 (data from the City of Sacramento, CA, Water Quality Laboratory; personal

communication). Units Are mg/L.

Date pH Hardness Alkalinity Ca Mg Ca:Mg Na Cl SO 4 

### Jul-78 7.6 20 22 5.2 1.7 3.06 3.2 2.6 4 Aug-78 7.6 20 22 4.9 1.9 2.58 3.4 2.8 5 Sep-78 7.5 20 22 5.2 1.7 3.06 3.5 2.6 4

Oct-78 7.3 20 22 5 1.8 2.78 3.6 3 4

Nov-78 7.2 20 4.9 1.9 2.58 3.9 5

Dec-78

Jan-79 7.4 23 24 5.1 2.1 2.43 3.2 2.9 4

Feb-79 7.5 24 25 6.5 1.9 3.42 3 3 5

Mar-79 7.6 26 27 7.4 1.8 4.11 3.3 2.7 6 Apr-79 7.7 27 27 7.5 2 3.75 3.6 2.7 7
May-79 7.6 25 26 5.7 2.6 2.19 3.4 2.4 6

Jun-79 7.7 22 24 5.7 1.9 3.00 3.1 2.5 4

Jul-79 7.6 21 22 5.3 1.9 2.79 3 2.7 4 Aug-79 7.5 21 22 5.6 1.7 3.29 3.2 2.4 5 Sep-79 7.3 20 21 5.7 1.4 4.07 3.5 2.5 3

Oct-79 7.2 19 20 5.5 1.3 4.23 3.1 2.8 3

Nov-79

Dec-79

Jan-80 7.5 23 23 6.1 1.9 3.21 2.4 2.6 4

Feb-80 7.4 23 23 6.1 1.9 3.21 2.7 2.3 2

Mar-80 7.5 24 26 5.8 2.3 2.52 2 2.3 2 Apr-80 7.7 25 25 6.4 2.2 2.91 1.9 2.5 3 May-80 7.5 22 21 6.1 1.6 3.81 2.4 2.4 3

Jun-80 7.3 19 21 5.1 1.5 3.40 2.3 2.4 2

Jul-80 7.4 18 20 4.6 1.6 2.88 2.6 2.1 3 Aug-80 7.5 18 21 5.2 1.2 4.33 3 2.7 2 Sep-80 7.3 18 20 4.9 1.4 3.50 2.9 2.4 4

Oct-80 7.3 18 20 5 1.3 3.85 3 2.7 2

Mean 7.5 21.4 22.8 5.6 1.8 3.2 3.0 2.6 3.8

max 7.7 27.0 27.0 7.5 2.6 4.3 3.9 3.0 7.0

min 7.2 18.0 20.0 4.6 1.2 2.2 1.9 2.1 2.0

### C-53 

# Appendix C -10. STORET Data for Minnesota Lakes and Rivers

Date pH Hardness Alkalinity Ca Mg Ca:Mg Na K Cl SO 4 

### NO 3 

### TOC DOC Sulfide
Embarrass River, MN

3/22/76 7 133 103 27 16 1.69 2.5 2 11 34

4/29/76 6.7 25.3 23 5.2 3 1.73 2.8 0.7 2.9 8.4 0.04 16 0.6

5/28/76 6.5 53 3.5 12

6/28/76 6.9 44 36 9.9 4.6 2.15 3.9 0.3 5 13 0.04 37

7/28/76 6.6 76 5.2 4.8 7.5

8/26/76 6.9 100 110 24 9.9 2.42 9 1 8.4 5.6 21 0.6

Means 6.8 75.58 66.83 14.26 8.38 2.00 4.55 1.00 5.93 13.42 0.04 24.67 0.60

max. 7 133 110 27 16 2.42 9 2 11 34 0.04 37 0.6

min. 6.5 25.3 23 5.2 3 1.69 2.5 0.3 2.9 5.6 0.04 16 0.6

S. Kawishiwi River, MN

10/16/75 6.4 21 14 4.9 2.1 2.33 1.3 0.4 0.5 4.4 0.01 12 0.2

11/6/75 6.9 24 19 5.5 2.5 2.20 1.2 0.4 0.6 4.1

12/11/75 39 23 10 3.4 2.94 1.4 0.4 1.5 0.2

1/9/76 6.6 29 24 6.2 3.2 1.94 1.6 0.8 2.3 7

2/4/76 6.3 24 20 5.2 2.7 1.93 1.7 0.6 0.9 6.3 0.16 16 0

3/9/76 6.9 23 23 5.7 2.2 2.59 1.5 0.5 0.9 4.9 1

4/23/76 6.6 14 8 3.4 1.3 2.62 0.9 0.4 0.7 4.8 0.2

5/25/76 6.8 16 11 4 1.5 2.67 0.9 0.4 0.7 4.8

6/25/76 6.6 16 1.1 3.3 1.8

7/23/76 6.7 19 1.2 4.4 0.5

Means 6.6 23.75 17.70 5.61 2.36 2.40 1.31 0.49 1.04 4.89 0.09 14.00 0.56

max. 6.9 39 24 10 3.4 2.94 1.7 0.8 2.3 7 0.16 16 1.8

min. 6.3 14 8 3.4 1.3 1.93 0.9 0.4 0.5 3.3 0.01 12 0

Colby Lake, MN

LCY2

6/17/96 8.5 56 33 13 5.7 2.28 4.3 1.5 6.3 22 0.25 17

6/17/96 6.8 0.25 17

6/17/96 6.9 71 33 17 7 2.43 4.3 1.4 9.4 22 18

LCY1

6/17/96 6.8 54 33 12 5.8 2.07 3.9 1.4 6.6 26 0.3 16

6/17/96 6.8 16

6/17/96 6.5 41 34 11 3.2 3.44 3.6 1.3 6.8 22 0.33 17

6/17/96 7.4 83 39 21 7.3 2.88 7.8 52 0.18

Means 7.1 55.50 33.25 13.25 5.43 2.55 4.03 1.40 7.28 23.00 0.28 16.83

max. 8.5 71 34 17 7 3.44 4.3 1.5 9.4 26 0.33 18

min. 6.5 41 33 11 3.2 2.07 3.6 1.3 6.3 22 0.25 16

Cloquet Lake, MN

7/13/76 6.4 17 11 4 1.8 2.22 1.7 7.6 0 38

Lake One, MN

10/16/75 7.2 27 21 6.9 2.3 3.00 1.2 5.6 0.02 22

Greenwood Lake, MN

7/6/76 6.7 10 15 2.8 0.7 4.00 0.1 0.3 0.2 4.2 0 11

# Appendix D. Saltwater Conversion Factors for Dissolved Values

### D -1 

# Appendix D

Saltwater Conversion Factors for Dissolved Values

February 14, 2007

U.S. Environmental Protection Agency

Office of Water Office of Science and Technology Washington, D.C.

### D -2 

# ACKNOWLEDGMENTS

Larry Brooke and Tyler Linton (primary authors)

Great Lakes Environmental Center 1295 King Avenue Columbus, OH 43212

Jennifer Mitchell and Cindy Roberts (authors and document coordinators) U.S. Environmental Protection Agency Washington, DC

### D -3



# Saltwater Conversion Factors for Converting Nominal or Total Copper Concentrations to Dissolved Copper Concentrations

The U.S. EPA changed its policy in 1993 of basing water quality criteria for metals from a total metal criteria to a dissolved metal criteria. The policy states “the use of dissolved metal to set and measure compliance with water quality standards is the recommended approach, because dissolved metal more closely approximates the bioavailable fraction of metal in the water column than does total recoverable metal” (Prothro 1993). All of the criteria for metals to this date were based upon total metal and very few data were available with dissolved concentrations of the metals. A problem was created by the new policy of how to derive dissolved metal concentrations for studies in which this form of the metal was not measured. The U.S. EPA attempted to develop correction factors for each metal for which criteria exist for both fresh- and saltwater (Lussier et al. 1995; Stephan 1995). In the case of saltwater, a correction for copper was not derived.

Several saltwater studies are available that report nominal, total, and dissolved concentrations of copper in laboratory water (Table 1) from site-specific water effect ratio (WER) studies. These studiesshow relatively consistent ratios for the nominal-to-dissolved concentrations and for the total-todissolved concentrations. Calculation of a mean ratio (conversion factor) to convert nominal and total copper concentrations to dissolved copper permits the use of the results for critical studies without dissolved copper measurements.

Three studies, each with multiple tests per study, were useful for deriving the conversion factors. One study was conducted for the lower Hudson River in the New York/New Jersey Harbor (SAIC 1993). The tests were conducted with harbor site water and with EPA Environmental Research Laboratory - Narragansett water from Narragansett Bay, Massachusetts. Only the tests with laboratory water were used for this exercise. Three series of 48-hour static tests were conducted with various animals. Salinityranged from 28 to 32 ppt during all the tests. Series 1 tests were not used to calculate ratios for dissolvedto-total or dissolved-to-nominal copper concentrations, because in many instances, concentrations of measured copper did not increase as nominal concentrations increased. Of the series 2 tests, only the coot clam ( Mulinia lateralis ) tests were successful and used to calculate ratios. Three replicate tests without ultraviolet (UV) light present and one test with UV light present were reported with total and dissolvedcopper measurements made at 0 hr and 48 hr (end) of the tests. Dissolved-to-total and dissolved-tonominal ratios were calculated for the four tests each with two time intervals. The mean ratio for the

dissolved-to-total measurements is 0.943 and the mean ratio for the dissolved-to-nominal is 0.917. A third series of static tests was conducted by SAIC and the mussel ( Mytilus sp. ) test was the only successful test. Again the tests were conducted as three replicate tests without UV light and a fourth with UV light. The mean test ratio for dissolved-to-total copper was 0.863 and the dissolved-to-nominal mean

test ratio was 0.906.

The summer flounder ( Paralichthys dentatus ) was exposed to copper in laboratory water for 96 hours in a static test (CH2MHill 1999a). The water was collected from Narragansett Bay and diluted with laboratory reverse osmosis water to dilute the solution to 22 ppt salinity. Three tests were run with copper concentrations measured at the start of the tests as total recoverable and dissolved copper. Five exposure concentrations were used to conduct the tests. Only the two lowest concentrations were used to derive ratios for dissolved-to-total and dissolved-to-nominal copper mean ratios. These concentrations were at the approximate 500 μg/L or lower concentrations, and are in the range of most copper concentrations routinely tested in the laboratory. The mean dissolved-to-total and dissolved-to-nominal ratios were 0.947 and 0.836, respectively.

Three 48-hour static tests were conducted with the blue mussel ( Mytilus edulis ) in water from the

### D -4



# same source and treated in the same manner as the summer flounder tests (CH2MHill 1999b). Salinity was diluted to 20 ppt. Exposures were made at eight concentrations of copper and total and dissolved copper concentrations were measured only at the start of the tests. Mean ratios for the dissolved-to-total and dissolved-to-nominal copper were calculated by combining the ratios calculated for each of the test concentrations. The mean dissolved-to-total and dissolved-to-nominal ratios were 0.979 and 0.879, respectively.

A study was conducted by the City of San Jose, CA to develop a WER for San Francisco Bay in which copper was used as a toxicant and the concentrations used in the laboratory exposures were measured as total and dissolved copper (Environ. Serv. Dept., City of San Jose 1998). Mussels and the purple sea urchin ( Strongylocentrotus purpuratus ) were used as the test organisms. Tests were conducted in filtered natural sea water from San Francisco Bay that was diluted to a salinity of 28 ppt. The mussel test was of 48-hour duration and the purple sea urchin test was of 96-hour duration. Five concentrations of copper were used in the toxicity tests with the concentrations measured at the start of each test. (During each test, a single concentration of copper was measured at the termination of the test and this value was not used in the calculations.) Twenty-two tests were conducted during a 13-month period with the mussel and two tests were conducted with the purple sea urchin. The mean dissolved-to-total anddissolved-to-nominal ratios for the mussel tests were 0.836 and 0.785, respectively. The mean dissolvedto-total and dissolved-to-nominal ratios for the purple sea urchin were 0.883 and 0.702, respectively.

For some of the tests, control concentrations had measured concentrations of total and dissolved copper. These values were not used to calculate ratios for dissolved-to-total and dissolved-to-nominal copper concentrations. All mean ratios were calculated as the arithmetic mean and not as a geometric mean of the available ratios. When the data are normally distributed, the arithmetic mean is the appropriate measure of central tendency (Parkhurst 1998) and is a better estimator than the geometric mean. All concentrations of copper used to calculate ratios should be time-weighted averages (Stephan 1995). In all instances of data used to calculate ratios, the concentrations were identical to time-weighted values because either only one value was available or if two were available they were of equal weight.

Based on the information presented above the overall ratio for correcting total copper concentrations to dissolved copper concentrations is 0.909 based upon the results of six sets of studies. This is comparable to its equivalent factor in freshwater, which is 0.960 ± 0.037 (Stephan 1995). When it is necessary to convert nominal copper concentrations to dissolved copper concentrations the conversion factor is 0.838 based upon the same studies. The means of both conversion factors have standard deviations of less than ten percent of the means (Table 1).

### D -5



# Table D -1. Summary of Saltwater Copper Ratios

Species Mean Dissolved-toTotal Ratio Mean Dissolved-toNominal Ratio Reference

Coot clam,

Mulinia lateralis 0.943 0.917 SAIC 1993

Summer flounder, Paralichthys dentatus 0.947 0.836 CH2MHill 1999a

Blue mussel, Mytilus sp 0.863 0.906 SAIC 1993

Blue mussel, Mytilus edulis 0.979 0.879 CH2MHill 1999b

Blue mussel, Mytilus sp 0.836 0.785 Environ. Serv. Dept., City of San Jose 1998

Purple sea urchin, Strongylocentrotus

purpuratus 0.883 0.702 Environ. Serv. Dept., City of San Jose 1998

Arithmetic Mean 0.909 0.838

Standard Deviation ±0.056 ±0.082

### D -6



# References

CH2MH ill. 1999a. Bioassay report: Acute toxicity of copper to summer flounder ( Paralichthys dentatus ). Final report prepared for U.S. Navy. November 1999. CH2MHill, Norfolk, Virginia. 26 p.

CH2MHill. 1999b. Bioassay report: Acute toxicity of copper to blue mussel ( Mytilus edulis ). Final report prepared for U.S. Navy. November 1999. CH2MHill, Norfolk, Virginia. 41 p.

Environmental Services Department, City of San Jose. 1998. Development of a site-specific water quality criterion for copper in south San Francisco Bay. Environmental Services Department, City of San Jose, San Jose/Santa Clara Water Pollution Control Plant, 4245 Zanker Road, San Jose, CA. 171 pp. May.

Lussier, S.M., W.S. Boothman, S. Poucher, D. Champlin and A. Helmsteter. 1995. Derivation of conversion factors for dissolved saltwater aquatic life criteria for metals. Draft report to the U.S. EPA, Office of Water. U.S. EPA, Narragansett, RI. March 31, 1995.

Parkhurst, D.F. 1998. Arithmetic versus geometric means for environmental concentration data. Environ. Sci.

Technol./News. 32:92A-95A.

Prothro, M. 1993. Memorandum concerning “Office of Water Policy and Technical Guidance on Interpretation and Implementation of Aquatic Life Metals Criteria.” October 1.

SAIC. 1993. Toxicity testing to support the New York/New Jersey Harbor site-specific copper criteria study. Final Report to U.S. EPA, Office of Wastewater Enforcement and Compliance (Contract No. 68-C8-0066. Work Assignment C-4-94). Science Applications International Corporation, Narragansett, RI.

Stephan, C.E. 1995. Derivation of conversation factors for the calculation of dissolved freshwater aquatic life criteria for metals. Report. March 11, 1995. U.S. EPA, Duluth, MN.

# Appendix E. BLM Input Data and NotesAppendix E. BLM TableModel OutputModel InputBLMData LabelCriticalAccumulationHardness(mg/L)Temp(oC) pHDissolvedLC50 (μg/L)DOC(mg/L)HumicAcid (%)Ca(mg/L)Mg(mg/L)Na(mg/L)K(mg/L)SO4(mg/L)Cl(mg/L)Alkalinity(mg/L)S(mg/L)NotesLUVA01S 1.1869 290 25 6.57 124.8 0.5 10 47.8602 41.47 89.821 7.178 278.4 6.5081 235 0.0003 1,2,3,4,5LUVA02S 2.1707 290 25 7.29 259.2 0.5 10 47.8602 41.47 89.821 7.178 278.4 6.5081 235 0.0003 1,2,3,4,5LUVA03S 2.0991 290 25 8.25 480 0.5 10 47.8602 41.47 89.821 7.178 278.4 6.5081 235 0.0003 1,2,3,4,5CADE01F 27.6903 44.9 15 7.7 1920 1.1 10 13.1965 2.911001 1.27 0.56 3.32 1.2 42.7 0.0003 1,2,3,6,7,8CADE02F 26.6895 44.9 15 7.7 1344 1.1 10 13.1965 2.911001 1.27 0.56 3.32 1.2 42.7 0.0003 1,2,3,6,7,8JUPL01F 0.1537 21 15 7.20 14.4 1.1 10 6.0583 1.7462 4.5302 0.7 2.8706 5.468 26 0.0003 1,3,6,7,9,10LIVI01F 0.0570 21 15 7.2 7.68 1.1 10 6.0583 1.7462 4.5302 0.7 2.8706 5.468 26 0.0003 1,3,6,7,9,10PHIN01F 0.4378 44.9 15 7.7 39.36 1.1 10 13.1965 2.911001 1.27 0.56 3.32 1.2 42.7 0.0003 1,2,3,6,7,8PHIN02F 0.3410 44.9 15 7.7 35.52 1.1 10 13.1965 2.911001 1.27 0.56 3.32 1.2 42.7 0.0003 1,2,3,6,7,8ACPE01S 0.1147 96 25 8.35 25.92 0.5 10 15.8434 13.728 29.734 2.3762 92.159 2.1544 102 0.0003 1,2,3,4,6,7,20ACPE02S 0.1556 68 25 8.35 27.84 0.5 10 11.2224 9.724 21.061 1.6831 65.279 1.526 108 0.0003 1,2,3,4,6,7,20UTIM01S 8.2925 39 23 7.4 82.56 0.5 10 6.43638 5.577 12.079 0.9653 37.439 0.8752 32.5 0.0003 1,2,3,4,6,11UTIM02S 8.0633 90 23 7.6 191.04 0.5 10 13.9716 12.11764 26.253 2.098 81.372 1.9022 65 0.0003 1,2,3,4,12UTIM03S 1.3555 92 25 8.1 72.96 0.5 10 29.0614 4.73839 30.798 1.6408 46.006 32.716 77 0.0003 1,2,3,4,6,7,53UTIM04S 1.4793 86 25 8.2 81.6 0.5 10 27.1661 4.429364 28.79 1.5338 43.005 30.583 78 0.0003 1,2,3,4,6,7,53UTIM05S 0.5289 90 25 8 39.36 0.5 10 28.4296 4.635381 30.129 1.6052 45.006 32.005 78 0.0003 1,2,3,4,6,7,53UTIM06S 1.2514 90 24 8.2 75.84 0.5 10 14.8532 12.87 13.938 1.1138 43.199 1.0099 99 0.0003 1,2,3,4,5,6,7UTIM07S 1.3009 90 25 7.9 69.12 0.5 10 28.4296 4.635381 30.129 1.6052 45.006 32.005 99 0.0003 1,2,3,4,6,7,53UTIM08S 0.7111 86 25 7.9 36.48 0.5 10 14.193 12.298 13.318 1.0643 41.279 0.965 59 0.0003 1,2,3,4,5,6,7CEDU01S 0.1132 52 24.5 7.5 18.24 1.1 10 15.2833 3.371316 1.5 0.57 3.8 1.4 55 0.0003 1,2,3,6,7,8CEDU02S 0.0941 52 24.5 7.5 16.32 1.1 10 15.2833 3.371316 1.5 0.57 3.8 1.4 55 0.0003 1,2,3,6,7,8CEDU03S 0.0751 45 25 7.72 25 1.5 10 11.0991 4.2075 9.5 1.6 46 34 39.7 0.0003 1,2,6,7,16CEDU04S 0.0400 45 25 7.72 17 1.5 10 11.0991 4.2075 9.5 1.6 46 34 39.7 0.0003 1,2,6,7,16CEDU05S 0.1046 45 25 7.72 30 1.5 10 11.0991 4.2075 9.5 1.6 46 34 39.7 0.0003 1,2,6,7,16CEDU06S 0.0700 45 25 7.72 24 1.5 10 11.0991 4.2075 9.5 1.6 46 34 39.7 0.0003 1,2,6,7,16CEDU07S 0.0920 45 25 7.72 28 1.5 10 11.0991 4.2075 9.5 1.6 46 34 39.7 0.0003 1,2,6,7,16CEDU08S 0.1184 45 25 7.72 32 1.5 10 11.0991 4.2075 9.5 1.6 46 34 39.7 0.0003 1,2,6,7,16CEDU09S 0.0651 45 25 7.72 23 1.5 10 11.0991 4.2075 9.5 1.6 46 34 39.7 0.0003 1,2,6,7,16CEDU10S 0.0517 45 25 7.72 20 1.5 10 11.0991 4.2075 9.5 1.6 46 34 39.7 0.0003 1,2,6,7,16CEDU11S 0.0476 45 25 7.72 19 1.5 10 11.0991 4.2075 9.5 1.6 46 34 39.7 0.0003 1,2,6,7,16CEDU12S 0.0194 94.1 25 8.15 26 2 10 23.2094 8.79835 5.2449 1.6 20.054 6.1705 69.6 0.0003 1,2,6,7,17CEDU13S 0.0144 94.1 25 8.15 21 2 10 23.2094 8.79835 5.2449 1.6 20.054 6.1705 69.6 0.0003 1,2,6,7,17CEDU14S 0.0206 94.1 25 8.15 27 2 10 23.2094 8.79835 5.2449 1.6 20.054 6.1705 69.6 0.0003 1,2,6,7,17E-1Appendix E. BLM TableModel OutputModel InputBLMData LabelCriticalAccumulationHardness(mg/L)Temp(oC) pHDissolvedLC50 (μg/L)DOC(mg/L)HumicAcid (%)Ca(mg/L)Mg(mg/L)Na(mg/L)K(mg/L)SO4(mg/L)Cl(mg/L)Alkalinity(mg/L)S(mg/L)NotesCEDU15S0.0338 94.1 25 8.15 37 2 10 23.2094 8.79835 5.2449 1.6 20.054 6.1705 69.6 0.0003 1,2,6,7,17CEDU16S 0.0294 94.1 25 8.15 34 2 10 23.2094 8.79835 5.2449 1.6 20.054 6.1705 69.6 0.0003 1,2,6,7,17CEDU17S 0.0428 179 25 8.31 67 2.3 10 50.1069 13.12323 14.32 2.4 22.673 10.979 140.1 0.0003 1,2,6,7,18CEDU18S 0.0164 179 25 8.31 38 2.3 10 50.1069 13.12323 14.32 2.4 22.673 10.979 140.1 0.0003 1,2,6,7,18CEDU19S 0.0579 179 25 8.31 78 2.3 10 50.1069 13.12323 14.32 2.4 22.673 10.979 140.1 0.0003 1,2,6,7,18CEDU20S 0.0627 179 25 8.31 81 2.3 10 50.1069 13.12323 14.32 2.4 22.673 10.979 140.1 0.0003 1,2,6,7,18CEDU21S 0.0283 97.6 25 8 28 2 10 24.0727 9.1256 5.44 1.6 20.8 6.4 74.2 0.0003 1,2,6,7,17CEDU22S 0.1218 182 25 8 84 2.3 10 50.9467 13.34317 14.56 2.4 23.053 11.163 144.3 0.0003 1,2,6,7,18CEDU23S 0.0510 57.1 25 8.18 12.864 0.5 10 9.42352 8.1653 17.685 1.4133 54.815 1.2814 81 0.0003 1,2,3,4,6,7,20CEDU24R 0.0377 80 20 7.6 5.5396825 0.5 10 13.2028 11.44 24.778 1.9801 76.799 1.7953 53 0.0003 1,2,6,7,20,21DAMA01S 0.0221 39 20 7.8 8.736 1.1 10 10.9867 2.7776 5.8136 0.7 7.9394 7.7684 51 0.0003 1,2,3,6,7,9,10DAMA02S 0.0315 39 20 7.8 11.232 1.1 10 10.9867 2.7776 5.8136 0.7 7.9394 7.7684 51 0.0003 1,2,3,6,7,9,10DAMA03S 0.0147 38 20 7.79 6.336 1.1 10 10.7129 2.7203 5.7423 0.7 7.6578 7.6406 50 0.0003 1,2,3,6,7,9,10DAMA04S 0.0253 38 20 7.79 9.504 1.1 10 10.7129 2.7203 5.7423 0.7 7.6578 7.6406 50 0.0003 1,2,3,6,7,9,10DAMA05S 0.1799 39 20 6.9 11.232 1.1 10 10.9867 2.7776 5.8136 0.7 7.9394 7.7684 30 0.0003 1,2,3,6,7,9,10DAMA06S 0.0786 39 20 6.9 6.432 1.1 10 10.9867 2.7776 5.8136 0.7 7.9394 7.7684 30 0.0003 1,2,3,6,7,9,10DAMA07S 0.0312 26 20 7.6 8.736 1.1 10 7.4273 2.0327 4.8867 0.7 4.2786 6.107 24 0.0003 1,2,3,6,7,9,10DAMA08S 0.0123 27 20 7.7 4.992 1.1 10 7.7011 2.09 4.958 0.7 4.5602 6.2348 24 0.0003 1,2,3,6,7,9,10DAMA09S 0.4278 170 20 7.8 39.552 0.5 10 27.9433 24.23527 52.507 4.1961 162.74 3.8045 115 0.0003 3,4,22,23DAMA10S 0.0443 170 20 7.8 10.08 0.5 10 27.9433 24.23527 52.507 4.1961 162.74 3.8045 115 0.0003 3,4,22,23DAMA11S 0.1330 170 20 7.8 19.776 0.5 10 27.9433 24.23527 52.507 4.1961 162.74 3.8045 115 0.0003 3,4,22,23DAMA12S 0.0990 170 20 7.8 16.608 0.5 10 27.9433 24.23527 52.507 4.1961 162.74 3.8045 115 0.0003 3,4,22,23DAMA13S 0.9670 170 20 7.8 67.872 0.5 10 27.9433 24.23527 52.507 4.1961 162.74 3.8045 115 0.0003 3,4,22,23DAMA14S 0.2716 170 20 7.8 30.048 0.5 10 27.9433 24.23527 52.507 4.1961 162.74 3.8045 115 0.0003 3,4,22,23DAMA15S 0.0160 109.9 21 6.93 6.816 2.4 10 40.0 2.43 85.1 1.23 10 106 12.5 0.0003 1,2,3,6,7,24DAMA16S 0.0298 109.9 21 6.93 15.744 3.4 10 40.0 2.43 85.1 1.23 10 106 12.5 0.0003 1,2,3,6,7,24DAMA17S 0.0393 109.9 21 7.43 38.304 3.4 10 40.0 2.43 85.1 1.23 10 106 13.875 0.0003 1,2,3,6,7,19,24DAMA18S 0.0219 109.9 21 7.43 17.952 2.4 10 40.0 2.43 85.1 1.23 10 106 13.875 0.0003 1,2,3,6,7,19,24DAMA19S 0.0111 109.9 21 7.82 18.144 2.4 10 40.0 2.43 85.1 1.23 10 106 14.5 0.0003 1,2,3,6,7,19,24DAMA20S 0.0189 109.9 21 7.82 38.112 3.4 10 40.0 2.43 85.1 1.23 10 106 14.5 0.0003 1,2,3,6,7,19,24DAMA21S 0.0898 109.9 21 6.93 44.16 4.4 10 40.0 2.43 85.1 1.23 10 106 12.5 0.0003 1,2,3,6,7,24DAMA22S 0.1076 109.9 21 6.93 69.024 6.1 10 40.0 2.43 85.1 1.23 10 106 12.5 0.0003 1,2,3,6,7,24DAMA23S 0.0458 109.9 21 7.43 54.912 4.4 10 40.0 2.43 85.1 1.23 10 106 13.875 0.0003 1,2,3,6,7,19,24DAMA24S 0.0288 109.9 21 7.82 65.088 4.4 10 40.0 2.43 85.1 1.23 10 106 14.5 0.0003 1,2,3,6,7,19,24E-2Appendix E. BLM TableModel OutputModel InputBLMData LabelCriticalAccumulationHardness(mg/L)Temp(oC) pHDissolvedLC50 (μg/L)DOC(mg/L)HumicAcid (%)Ca(mg/L)Mg(mg/L)Na(mg/L)K(mg/L)SO4(mg/L)Cl(mg/L)Alkalinity(mg/L)S(mg/L)NotesDAMA25S0.1143 52 18.2 7.8 24.96 1.1 10 14 3.5 12 2.9 23 11 45 0.0003 1,2,3,6,7,9,25DAMA26S 0.0917 105 20.3 7.9 28.8 1.1 10 29 6.8 29 5.3 57 21 79 0.0003 1,2,3,6,7,9,25DAMA27S 0.1053 106 19.7 8.1 36.48 1.1 10 29 6.8 29 5.3 57 21 82 0.0003 1,2,3,6,7,9,25DAMA28S 0.1538 207 19.9 8.3 66.24 1.1 10 58 13 62 8.2 127 40 166 0.0003 1,2,3,6,7,9,25DAMA29S 0.0062 7.1 24 8.55 4.608 0.5 10 1.15182 1.027387 3.5102 2.8052 6.8159 2.5434 56 0.0003 1,2,3,4,6,7,56DAMA30S 0.2536 20.6 24 6.97 7.104 0.5 10 3.39973 2.9458 2.5478 2.1356 19.776 1.9363 60 0.0003 1,2,3,4,6,7,56DAMA31S 0.0119 23 24 8.52 6.24 0.5 10 3.79581 3.289 2.8446 2.3845 22.08 2.1619 64 0.0003 1,2,3,4,6,7,56DAPC01S 0.0087 48 18 8.03 10.944 2.288 10 14.1077 3.111984 1.36 0.57 3.55 1.25 42 0.0003 1,2,3,6,7,15,26DAPC02S 0.0052 48 18 8.03 8.6976 2.816 10 14.1077 3.111984 1.36 0.57 3.55 1.25 42 0.0003 1,2,3,6,7,15,26DAPC03S 0.0043 48 18 8.01 6.9504 2.728 10 14.1077 3.111984 1.36 0.57 3.55 1.25 44 0.0003 1,2,3,6,7,15,26DAPC04S 0.0057 44 18 8.04 10.368 3.08 10 12.932 2.852652 1.24 0.57 3.25 1.15 42 0.0003 1,2,3,6,7,15,26DAPC05S 0.0879 31 18 6.66 53.184 12.2094 10 7.37407 3.063455 1.6792 0.5 6.3292 1.2917 27 0.0003 1,2,3,6,7,27,28DAPC06S 0.0490 29 18 6.97 53.088 11.3373 10 6.89832 2.865813 1.5708 0.5 5.9208 1.2083 27 0.0003 1,2,3,6,7,27,28DAPC07S 0.0285 28 18 7.2 51.168 11.3373 10 6.66045 2.766992 1.5167 0.5 5.7167 1.1667 22 0.0003 1,2,3,6,7,27,28DAPC08S 0.0268 88 18 7.01 93.312 24.4188 10 20.9464 8.5194 16.466 1.8787 22.629 18.986 20 0.0003 1,2,3,6,7,27,29DAPC09S 0.0187 100 18 7.55 191.04 29.6514 10 23.9296 9.4686 21.207 2.1631 25.98 23.28 20 0.0003 1,2,3,6,7,27,29DAPC10S 0.0701 82 18 6.99 204.48 27.9072 10 19.4548 8.0448 14.095 1.7365 20.953 16.84 18 0.0003 1,2,3,6,7,27,29DAPC11S 0.0460 84 18 7.01 158.4 27.9072 10 19.952 8.203 14.885 1.7839 21.512 17.555 17 0.0003 1,2,3,6,7,27,29DAPC12S 0.0100 16 18 7.39 34.08 11.6124 10 4.13844 1.379481 0.16 0.3 6.72 0.32 11 0.0003 1,2,3,6,7,27,28DAPC13S 0.0137 151 18 7.76 75.648 12.5801 10 36.7872 14.39533 10.786 1.4 62.018 19.684 44 0.0003 1,2,3,6,7,27,28DAPC14S 0.0053 96 18 8.1 108.48 27.0956 10 22.0888 9.939946 6.8571 1.4 19.911 4.2667 91 0.0003 1,2,3,6,7,27,28DAPC15S 0.0137 26 18 7.24 73.344 24.1925 10 7.37925 1.844812 0.26 0.3 11.624 2.6 4 0.0003 1,2,3,6,7,27,28DAPC16S 0.0564 84 18 7.08 81.312 12.5801 10 20.4644 8.008 6 1.4 34.5 10.95 13 0.0003 1,2,3,6,7,27,28DAPC17S 0.0633 92 18 7.22 176.64 20.3217 10 22.4134 8.770667 6.5714 1.4 37.786 11.993 19 0.0003 1,2,3,6,7,27,28DAPC18S 0.0056 47 18 8.03 8.928 2.728 10 13.8137 3.047151 1.33 0.57 3.47 1.23 42.5 0.0003 1,2,3,6,7,15,26DAPC19S 0.0119 97 18 8.03 17.088 2.728 10 34 2.9 1.3 0.57 51.3 1.2 42.5 0.0003 1,2,3,6,7,15,30DAPC20S 0.0160 147 18 8.03 22.752 2.728 10 54 2.9 1.3 0.57 99.3 1.2 42.5 0.0003 1,2,3,6,7,15,30DAPC21S 0.0168 247 18 8.03 26.208 2.728 10 94 2.9 1.3 0.57 147.3 1.2 42.5 0.0003 1,2,3,6,7,15,30DAPC22S 0.0171 97 18 8.03 24.192 2.728 10 13.6 15.2 1.3 0.57 51.3 1.2 42.5 0.0003 1,2,3,6,7,15,30DAPC23S 0.0155 147 18 8.03 24.096 2.728 10 13.6 27.5 1.3 0.57 99.3 1.2 42.5 0.0003 1,2,3,6,7,15,30DAPC24S 0.0133 247 18 8.03 24.096 2.728 10 13.6 51.9 1.3 0.57 147.3 1.2 42.5 0.0003 1,2,3,6,7,15,30SCSP01S 0.1034 52 24.5 7.5 17.28 1.1 10 15.2833 3.371316 1.47 0.57 3.84 1.36 55 0.0003 1,2,3,6,7,8GAPS01F 0.1153 44.9 15 7.7 21.12 1.1 10 13.1965 2.911001 1.27 0.57 3.32 1.17 42.7 0.0003 1,2,3,6,7,8GAPS02F 0.0888 44.9 15 7.7 18.24 1.1 10 13.1965 2.911001 1.27 0.57 3.32 1.17 42.7 0.0003 1,2,3,6,7,8E-3Appendix E. BLM TableModel OutputModel InputBLMData LabelCriticalAccumulationHardness(mg/L)Temp(oC) pHDissolvedLC50 (μg/L)DOC(mg/L)HumicAcid (%)Ca(mg/L)Mg(mg/L)Na(mg/L)K(mg/L)SO4(mg/L)Cl(mg/L)Alkalinity(mg/L)S(mg/L)NotesHYAZ01S0.1511 290 25 6.23 16.32 0.5 10 47.8602 41.47 89.821 7.178 278.4 6.5081 235 0.0003 1,2,3,4,5,13HYAZ02S 0.1074 290 25 7.51 23.04 0.5 10 47.8602 41.47 89.821 7.178 278.4 6.5081 235 0.0003 1,2,3,4,5,13HYAZ03S 0.2392 290 25 8.38 83.52 0.5 10 47.8602 41.47 89.821 7.178 278.4 6.5081 235 0.0003 1,2,3,4,5,13HYAZ04S 0.0794 20.5 21 7.15 23.328 2.8 10 5.1 1.9 5.3 0.8 9.3 10.0 6.7 0.0003 3,31HYAZ05S 0.0768 20.5 21 7.15 22.848 2.8 10 5.1 1.9 5.3 0.8 9.3 10.0 6.7 0.0003 3,31HYAZ06S 0.2314 20.6 21 7.14 7.872 0.5 10 5.3 1.8 5.5 0.8 7.0 9.7 11.0 0.0003 3,31HYAZ07S 0.3312 20.6 21 7.14 9.6 0.5 10 5.3 1.8 5.5 0.8 7.0 9.7 11.0 0.0003 3,31ACLY01S 29.5658 42 18.5 7.0 7968 1.1 10 12.3442 2.722986 1.3 0.57 3.4 1.2 47 0.0003 1,2,3,6,7,8CHDE01S 25.2731 44 20 7.40 709.44 0.5 10 6.99 6.06 13.1 1.05 40.7 0.951 32.5 0.0003 1,2,3,4,32,33SCPL01S 2.9865 167 22 7.6 153.6 0.5 10 27.5609 23.881 51.724 4.1335 160.32 3.7478 115 0.0003 1,2,3,4,6,7,20ONAP01S 0.9139 169 12 8 67.2 0.5 10 27.891 24.167 52.344 4.183 162.24 3.7927 117 0.0003 1,2,3,4,6,7,20ONCL01S 1.0007 169 12 8.1 76.8 0.5 10 27.891 24.167 52.344 4.183 162.24 3.7927 117 0.0003 1,2,3,4,6,7,20ONCL02S 0.5538 169 12 8.25 57.6 0.5 10 27.891 24.167 52.344 4.183 162.24 3.7927 117 0.0003 1,2,3,4,6,7,20ONCL03F 2.8512 205 13.7 7.73 367 3.3 10 49.8 19.6 4 0.64 10 0.44 178 0.0003 1,2,6,7,34ONCL04F 1.5731 69.9 13.7 8.54 186 1.5 10 18.4 5.8 1.405 0.2248 3.5126 0.1546 174 0.0003 1,2,6,7,35ONCL05F 0.4400 18 13.7 8.07 36.8 0.75 10 4.8 1.5 0.3618 0.0579 0.9045 0.0398 183 0.0003 1,2,6,7,35ONCL06F 1.9714 204 13.7 7.61 232 3.3 10 64.7 10.3 4.1005 0.6561 10.251 0.4511 77.9 0.0003 1,2,6,7,35ONCL07F 5.2514 83 13.7 7.4 162 1.7 10 20.4 7.8 1.6683 0.2669 4.1709 0.1835 70 0.0003 1,2,6,7,35ONCL08F 1.2778 31.4 13.7 8.32 73.6 0.94 10 7.9 2.7 0.6312 0.101 1.5779 0.0694 78.3 0.0003 1,2,6,7,35ONCL09F 0.3591 160 13.7 7.53 91 2.8 10 57.5 4.0 3.2161 0.5146 8.0402 0.3538 26.0 0.0003 1,2,6,7,35ONCL10F 0.3318 74.3 13.7 7.57 44.4 1.5 10 24.7 3.1 1.4935 0.239 3.7337 0.1643 22.7 0.0003 1,2,6,7,35ONCL11F 0.1192 26.4 13.7 7.64 15.7 0.87 10 6.0 2.8 0.5307 0.0849 1.3266 0.0584 20.1 0.0003 1,2,6,7,35ONGO01F 1.3932 83.1 7.15 7.63 137.28 2.58 10 22.3428 6.313221 10.259 7.5024 25.1 9.994 62.5 0.0003 1,2,3,6,7,52ONGO02F 0.3615 83.1 7.15 7.63 83.52 2.58 10 22.3428 6.313221 10.259 7.5024 25.1 9.994 62.5 0.0003 1,2,3,6,7,52ONGO03F 3.5018 83.1 7.15 7.63 191.04 2.58 10 22.3428 6.313221 10.259 7.5024 25.1 9.994 62.5 0.0003 1,2,3,6,7,52ONKI01R 4.9807 33 13.5 7.29 157.44 2.496 10 8.77741 2.698479 7.3188 1.15 6.1426 6.8124 29 0.0003 1,2,3,6,7,27,36ONKI02F 0.4054 25 12 7.30 31.68 1.3 10 6.8 1.8 5.0 0.6 4.2 6 24 0.0003 3,37ONKI03F 0.9203 20 9.4 7.29 44.16 1.3 10 5.7845 1.6889 4.4589 0.7 2.589 5.3402 22 0.0003 1,2,3,6,7,10,38ONKI04F 0.1617 31.1 13.3 7.30 49 3.2 10 8.01999 2.695987 5.12 0.653 4 4.5 29.6 0.0003 1,2,6,7,39ONKI05F 0.1736 31.1 13.3 7.30 51 3.2 10 8.01999 2.695987 5.12 0.653 4 4.5 29.6 0.0003 1,2,6,7,39ONKI06F 0.1461 31.6 15.7 7.50 58 3.2 10 8.14893 2.739331 5.12 0.653 3.5 4.2 30.4 0.0003 1,2,6,7,39ONKI07F 0.4829 31 15.3 7.20 78 3.2 10 7.99421 2.687318 5.12 0.653 2.3 3.1 29.7 0.0003 1,2,6,7,39ONMY01S 1.3925 169 12 8.2 105.6 0.5 10 27.891 24.167 52.344 4.183 162.24 3.7927 117 0.0003 1,2,3,4,6,7,20ONMY02S 0.5765 169 12 7.95 48 0.5 10 27.891 24.167 52.344 4.183 162.24 3.7927 117 0.0003 1,2,3,4,6,7,20E-4Appendix E. BLM TableModel OutputModel InputBLMData LabelCriticalAccumulationHardness(mg/L)Temp(oC) pHDissolvedLC50 (μg/L)DOC(mg/L)HumicAcid (%)Ca(mg/L)Mg(mg/L)Na(mg/L)K(mg/L)SO4(mg/L)Cl(mg/L)Alkalinity(mg/L)S(mg/L)NotesONMY03S0.7648 169 12 7.95 57.6 0.5 10 27.891 24.167 52.344 4.183 162.24 3.7927 117 0.0003 1,2,3,4,6,7,20ONMY04R0.1249 44.1 11.5 7.7 40 2 10 9.07 4.1 4.75 1.02 3.3 1.56 49.7 0.0003 40ONMY05R0.0917 44.6 11.5 7.8 19 0.99 10 7.37 6.1 6.24 0.8 1.31 3.82 53.1 0.0003 40ONMY06R0.0376 38.7 12 7.62 3.4 0.33 10 2.37 8.65 13.7 0.15 0.36 20.3 40 0.0003 51ONMY07R0.1465 39.3 12 7.61 8.1 0.36 10 14.1 1.8 13.2 0.1 0.36 19.9 41.7 0.0003 51ONMY08R0.1881 89.5 12 8.21 17.2 0.345 10 15 11.85 10.05 1 0.36 6.73 97.5 0.0003 51ONMY09R0.5172 89.67 12 8.15 32 0.345 10 28.9 3.15 32.5 0.5 0.36 45.2 97.25 0.0003 51ONMY10F0.3824 23 12.2 7.1 26.88 1.4 10 6.1 1.8 4.4 0.4 5.8 6 22 0.0003 3,37ONMY11F0.1589 23 12.2 7.1 16.32 1.4 10 6.1 1.8 4.4 0.4 5.8 6 22 0.0003 3,37ONMY12F0.1059 23 12.2 7.4 17.28 1.3 10 6.8 1.8 5.0 0.6 4.2 6 22 0.0003 3,37ONMY13F0.4633 23 12.2 7.1 27.84 1.3 10 6.8 1.8 5.0 0.6 4.2 6 22 0.0003 3,37ONMY14F0.4998 194 12.8 7.84 169 3.3 10 55.1 13.7 4 0.64 10 0.44 174 0.0003 1,2,6,7,34ONMY15F0.1118 194 12.8 7.84 85.3 3.3 10 55.1 13.7 4 0.64 10 0.44 174 0.0003 1,2,6,7,34ONMY16F0.1069 194 12.8 7.84 83.3 3.3 10 55.1 13.7 4 0.64 10 0.44 174 0.0003 1,2,6,7,34ONMY17F0.1627 194 12.8 7.84 103 3.3 10 55.1 13.7 4 0.64 10 0.44 174 0.0003 1,2,6,7,34ONMY18F1.5525 194 12.8 7.84 274 3.3 10 55.1 13.7 4 0.64 10 0.44 174 0.0003 1,2,6,7,34ONMY19F0.2605 194 12.8 7.84 128 3.3 10 55.1 13.7 4 0.64 10 0.44 174 0.0003 1,2,6,7,34ONMY20F0.9538 194 12.8 7.84 221 3.3 10 55.1 13.7 4 0.64 10 0.44 174 0.0003 1,2,6,7,34ONMY21F0.4717 194 12.8 7.84 165 3.3 10 55.1 13.7 4 0.64 10 0.44 174 0.0003 1,2,6,7,34ONMY22F0.7244 194 12.8 7.84 197 3.3 10 55.1 13.7 4 0.64 10 0.44 174 0.0003 1,2,6,7,34ONMY23F4.6605 194 12.8 7.84 514 3.3 10 55.1 13.7 4 0.64 10 0.44 174 0.0003 1,2,6,7,34ONMY24F1.1894 194 12.8 7.84 243 3.3 10 55.1 13.7 4 0.64 10 0.44 174 0.0003 1,2,6,7,34ONMY25F0.0613 9.2 15.5 6.96 2.688 0.5 10 2.3 0.7 2 0.2 4.6 2.1 11 0.0003 3,41ONMY26F0.3626 31 15.3 7.2 68 3.2 10 7.99421 2.687318 5.12 0.653 2.3 3.1 29.7 0.0003 1,2,6,7,39ONMY27F0.0770 36.1 11.4 7.6 18 1.31 10 4.03 7.13 1.56 0.26 1.49 0.88 36.6 0.0003 40ONMY28F0.8944 36.2 11.5 6.1 12 1.36 10 3.93 7.27 1.57 0.28 1.47 0.87 8.5 0.0003 40ONMY29F0.5568 20.4 11.7 7.5 5.7 0.15 10 3.13 2.77 2.62 0.25 0.36 1.48 23 0.0003 40ONMY30F0.2504 45.2 11.7 7.7 35 1.23 10 9.7 4.43 5.33 0.97 3.41 1.47 50 0.0003 40ONMY31F1.1775 45.4 11.8 6.3 18 1.22 10 9.7 4.43 5.02 0.98 3.37 1.37 10.9 0.0003 40ONMY32F0.5318 41.9 12.3 7.9 17 0.33 10 6.6 5.97 5.89 0.63 1.11 3.37 48.3 0.0003 40ONMY33F1.2884 214 7.64 7.94 96.96 0.27 10 49.4 24.1 10.3 1.75 18.9 5.28 198 0.0003 1,2,3,6,7,54,55ONMY34F3.8957 220 7.74 7.92 295.68 0.36 10 51.2 25.5 8.36 2.1 24 4.64 197 0.0003 1,2,3,6,7,54,55ONMY35F4.4437 105 7.77 7.82 89.28 0.1 10 23.1 11.8 3.54 3.22 17.1 2.91 94.1 0.0003 1,2,3,6,7,54,55ONMY36F1.9096 98.2 8.49 7.89 34.464 0.045 10 22.3 11.2 3.58 0.9 11.5 2.85 87.9 0.0003 1,2,3,6,7,54,55E-5Appendix E. BLM TableModel OutputModel InputBLMData LabelCriticalAccumulationHardness(mg/L)Temp(oC) pHDissolvedLC50 (μg/L)DOC(mg/L)HumicAcid (%)Ca(mg/L)Mg(mg/L)Na(mg/L)K(mg/L)SO4(mg/L)Cl(mg/L)Alkalinity(mg/L)S(mg/L)NotesONMY37F1.7297 104 16.3 7.83 52.224 0.28 10 22.4 11.4 3.76 2.72 12.4 3.01 97.6 0.0003 1,2,3,6,7,54,55ONNE01F3.1060 83.1 7.15 7.63 182.4 2.58 10 22.3428 6.313221 10.259 7.5024 25.1 9.994 62.5 0.0003 1,2,3,6,7,52ONNE02F3.5466 83.1 7.15 7.63 192 2.58 10 22.3428 6.313221 10.259 7.5024 25.1 9.994 62.5 0.0003 1,2,3,6,7,52ONNE03F0.5132 83.1 7.15 7.63 96 2.58 10 22.3428 6.313221 10.259 7.5024 25.1 9.994 62.5 0.0003 1,2,3,6,7,52ONNE04F0.6617 83.1 7.15 7.63 105.6 2.58 10 22.3428 6.313221 10.259 7.5024 25.1 9.994 62.5 0.0003 1,2,3,6,7,52ONNE05F1.0574 83.1 7.15 7.63 124.8 2.58 10 22.3428 6.313221 10.259 7.5024 25.1 9.994 62.5 0.0003 1,2,3,6,7,52ONNE06F1.6007 83.1 7.15 7.63 144 2.58 10 22.3428 6.313221 10.259 7.5024 25.1 9.994 62.5 0.0003 1,2,3,6,7,52ONNE07F4.0021 83.1 7.15 7.63 201.6 2.58 10 22.3428 6.313221 10.259 7.5024 25.1 9.994 62.5 0.0003 1,2,3,6,7,52ONNE08F2.2920 83.1 7.15 7.63 163.2 2.58 10 22.3428 6.313221 10.259 7.5024 25.1 9.994 62.5 0.0003 1,2,3,6,7,52ONNE09F3.1060 83.1 7.15 7.63 182.4 2.58 10 22.3428 6.313221 10.259 7.5024 25.1 9.994 62.5 0.0003 1,2,3,6,7,52ONNE10F5.4103 83.1 7.15 7.63 230.4 2.58 10 22.3428 6.313221 10.259 7.5024 25.1 9.994 62.5 0.0003 1,2,3,6,7,52ONTS01F0.2050 23 12.2 7.4 24.96 1.3 10 6.8 1.8 5.0 0.6 4.2 6 22 0.0003 3,37ONTS02F0.1161 23 12.2 7.4 18.24 1.3 10 6.8 1.8 5.0 0.6 4.2 6 22 0.0003 3,37ONTS03F0.7109 23 12.2 7.1 36.48 1.4 10 6.1 1.8 4.4 0.4 5.8 6 22 0.0003 3,37ONTS04F0.3750 23 12.2 7.1 24.96 1.3 10 6.8 1.8 5.0 0.6 4.2 6 22 0.0003 3,37ONTS05F0.3517 13 12 7.15 9.792 0.5 10 2.14546 1.859 4.0264 0.3218 12.48 0.2917 12 0.0003 1,2,3,4,6,7,20ONTS06F0.8340 46 12 7.55 23.136 0.5 10 7.59162 6.578 14.247 1.1386 44.159 1.0323 35 0.0003 1,2,3,4,6,7,20ONTS07F0.9241 182 12 8.12 79.2 0.5 10 30.0364 26.026 56.37 4.5048 174.72 4.0844 125 0.0003 1,2,3,4,6,7,20ONTS08F0.3954 359 12 8.49 123.264 0.5 10 59.2477 51.337 111.19 8.8858 344.64 8.0566 243 0.0003 1,2,3,4,6,7,20ONTS09F1.1161 36.6 12 7.71 7.4 0.055 10 6.36 4.73 4.84 0.22 0.94 2.79 40.8 0.0003 51ONTS10F0.8313 34.6 12 7.79 12.5 0.19 10 7.82 3.17 9.98 0.11 0.73 8.34 40.6 0.0003 51ONTS11F0.8622 38.3 12 7.71 14.3 0.24 10 6.33 5.1 5.27 0.6 0.99 2.96 43.6 0.0003 51ONTS12F1.7785 35.7 12 7.74 18.3 0.17 10 8.15 3.38 10 0.37 0.76 9.1 43.3 0.0003 51SACO01F2.9901 214 7.64 7.94 218.88 0.27 10 49.4 24.1 10.3 1.75 18.9 5.28 198 0.0003 1,2,3,6,7,54,55SACO02F2.6420 220 7.74 7.92 198.72 0.36 10 51.2 25.5 8.36 2.1 24 4.64 197 0.0003 1,2,3,6,7,54,55SACO03F3.2456 105 7.77 7.82 63.936 0.1 10 23.1 11.8 3.54 3.22 17.1 2.91 94.1 0.0003 1,2,3,6,7,54,55SACO04F2.6405 98.2 8.49 7.89 48 0.045 10 22.3 11.2 3.58 0.9 11.5 2.85 87.9 0.0003 1,2,3,6,7,54,55SACO05F3.0680 104 16.3 7.83 85.44 0.28 10 22.4 11.4 3.76 2.72 12.4 3.01 97.6 0.0003 1,2,3,6,7,54,55ACAL01F9.7513 54 10.5 7.3 137.28 1.1 10 15.0937 3.6371 6.8831 0.7 12.163 9.6854 43 0.0003 1,2,3,6,7,9,10GIEL01S2.6186 173 22 8.05 192 0.5 10 28.5511 24.739 53.583 4.282 166.08 3.8824 117 0.0003 1,2,3,6,7,20NOCR01F 29.9790 72.2 25 7.50 81216 1.5 10 17.8079 6.7507 15.26 1.6 73.841 54.15 42.5 0.0003 2,3,6,7,16,42PIPR01S11.3981 103 22 7.4 297.6 0.5 10 28.4667 7.773195 27.778 2.6358 29.602 53.021 65 0.0003 1,2,3,4,6,48PIPR02S 4.9570 103 22 7.4 115.2 0.5 10 28.4667 7.773195 27.778 2.6358 29.602 53.021 65 0.0003 1,2,3,4,6,48PIPR03S 9.4256 263 22 7.4 374.4 0.5 10 72.6868 19.84806 36.487 3.4623 77.901 130.77 65 0.0003 1,2,3,4,6,48E-6Appendix E. BLM TableModel OutputModel InputBLMData LabelCriticalAccumulationHardness(mg/L)Temp(oC) pHDissolvedLC50 (μg/L)DOC(mg/L)HumicAcid (%)Ca(mg/L)Mg(mg/L)Na(mg/L)K(mg/L)SO4(mg/L)Cl(mg/L)Alkalinity(mg/L)S(mg/L)NotesPIPR04S1.2005 52 24.5 7.4 52.8 1.1 10 15.2833 3.371316 1.47 0.57 3.84 1.36 55 0.0003 1,2,3,6,7,8PIPR05S3.0479 52 24.5 7.4 81.6 1.1 10 15.2833 3.371316 1.47 0.57 3.84 1.36 55 0.0003 1,2,3,6,7,8PIPR06S0.1314 290 25 6.27 14.4 0.5 10 47.8602 41.47 89.821 7.178 278.4 6.5081 235 0.0003 1,2,3,4,5PIPR07S0.3064 290 25 7.14 42.24 0.5 10 47.8602 41.47 89.821 7.178 278.4 6.5081 235 0.0003 1,2,3,4,5PIPR08S0.5392 290 25 8.6 192 0.5 10 47.8602 41.47 89.821 7.178 278.4 6.5081 235 0.0003 1,2,3,4,5PIPR09S0.0890 19 22 7.06 4.6272 0.6 10 4.9 1.64 3.7 0.78 9.6 5.8 11.17 0.0003 3,49PIPR10S0.2665 19.5 22 7.25 7.872 0.4 10 5.2 1.64 5.36 0.79 2.45 8.6 12.7 0.0003 3,49PIPR11S0.5716 16.5 22 6.36 30.3072 3.3 10 4.1 1.54 2.82 0.76 9.4 4.7 8.46 0.0003 3,49PIPR12S0.2950 17 22 6.42 20.2176 3.1 10 4.2 1.56 2.74 0.74 7.4 4.6 3.4 0.0003 3,49PIPR13S0.4162 19 22 6.38 34.5312 4.3 10 5 1.62 7.04 0.72 10.2 12.2 7.83 0.0003 3,49PIPR14S0.2640 17 22 7.15 57.4368 3.4 10 4.2 1.54 2.9 1 7.4 4.7 8.74 0.0003 3,49PIPR15S0.0477 17 22 7.16 4.6368 0.8 10 4.5 1.46 2.68 0.78 10.9 3.8 9.3 0.0003 3,49PIPR16S0.1770 17.5 22 7.13 67.4688 5.1 10 4.6 1.48 2.62 0.77 10.5 3.5 8.95 0.0003 3,49PIPR17S0.0787 18.5 22 7.06 80.2464 10.5 10 5 1.54 2.64 0.8 10.7 3.5 8.29 0.0003 3,49PIPR18S0.1907 18.5 22 6.90 174.72 15.6 10 4.9 1.5 3.54 0.99 7 5.2 9.52 0.0003 3,49PIPR19S3.2305 173 22 8.25 278.4 0.5 10 28.5511 24.739 53.583 4.282 166.08 3.8824 117 0.0003 1,2,3,4,6,7,20PIPR20S7.4512 173 22 8.1 604.8 0.5 10 28.5511 24.739 53.583 4.282 166.08 3.8824 117 0.0003 1,2,3,4,6,7,20PIPR21S4.8297 173 22 8.15 384 0.5 10 28.5511 24.739 53.583 4.282 166.08 3.8824 117 0.0003 1,2,3,4,6,7,20PIPR22S7.6122 173 22 7.3 374.4 0.5 10 28.5511 24.739 53.583 4.282 166.08 3.8824 117 0.0003 1,2,3,4,6,7,20PIPR23S7.2327 166 5 8.05 432 0.5 10 27.3959 23.738 51.415 4.1088 159.36 3.7253 132.5 0.0003 1,2,3,4,6,7,20PIPR24S3.4469 159 12 8.35 285.12 0.5 10 26.2406 22.737 49.247 3.9355 152.64 3.5682 135 0.0003 1,2,3,4,6,7,20PIPR25S2.8678 168 22 8.3 298.56 0.5 10 27.7259 24.024 52.034 4.1583 161.28 3.7702 142.5 0.0003 1,2,3,4,6,7,20PIPR26S3.3686 167 32 8.45 492.48 0.5 10 27.5609 23.881 51.724 4.1335 160.32 3.7478 140 0.0003 1,2,3,4,6,7,20PIPR27S0.5950 45.54059 22 7.93 53.958366 1.1 10 13.4911 2.888065 1.6093 0.391 3.362 1.4181 42.037464 0.0003 43,44PIPR28S4.0104 45.54059 22 7.93 165.17867 1.1 10 13.4911 2.888065 91.27 0.391 3.362 143.23 42.037464 0.0003 43,44PIPR29S0.7241 44.53969 22 7.98 59.464322 1.1 10 13.1946 2.824591 1.6093 0.391 3.362 1.4181 42.037464 0.0003 43,44PIPR30S4.0805 44.53969 22 7.98 146.45842 1.1 10 13.1946 2.824591 45.98 0.391 3.362 72.324 44.039248 0.0003 43,44PIPR31S1.8188 44.53969 22 7.99 82.038741 1.1 10 13.1946 2.824591 1.6093 0.391 3.362 1.4181 42.53791 0.0003 43,44PIPR32S4.9213 45.54059 22 7.96 124.4346 1.1 10 13.4911 2.888065 1.6093 0.391 3.362 36.871 43.038356 0.0003 43,44PIPR33S3.9367 45.04014 22 7.79 103.759 1.1 10 13.3428 2.856328 1.6093 0.391 3.362 1.4181 46.041032 0.0003 43,44PIPR34S5.7875 45.04014 22 7.81 167.3225 1.1 10 13.3428 2.856328 47.589 0.391 99.42 1.4181 46.041032 0.0003 43,44PIPR35S3.2914 138.1231 22 7.785 120.015 1.1 10 12.892 25.75825 1.6093 0.391 3.362 72.324 43.038356 0.0003 43,44PIPR36S5.7959 151.1347 22 7.78 169.418 1.1 10 14.1065 28.18476 1.6093 0.391 99.42 1.4181 43.038356 0.0003 43,44PIPR37S3.4870 138.1231 22 8.02 268.224 1.1 10 12.892 25.75825 1.6093 0.391 3.362 1.4181 149.13291 0.0003 43,44E-7Appendix E. BLM TableModel OutputModel InputBLMData LabelCriticalAccumulationHardness(mg/L)Temp(oC) pHDissolvedLC50 (μg/L)DOC(mg/L)HumicAcid (%)Ca(mg/L)Mg(mg/L)Na(mg/L)K(mg/L)SO4(mg/L)Cl(mg/L)Alkalinity(mg/L)S(mg/L)NotesPIPR38S9.2068 139.124 22 7.775 242.443 1.1 10 51.1778 2.779812 1.6093 0.391 99.42 1.4181 43.038356 0.0003 43,44PIPR39S4.7038 47.04192 22 7.78 113.3475 1.1 10 13.4268 4.010325 1.6093 0.391 3.362 1.4181 43.038356 0.0003 43,44PIPR40S3.1754 37.033 22 7.785 77.8764 0.88 10 11.022 3.281175 2.9887 0.391 3.362 1.4181 43.038356 0.0003 43,45PIPR41S5.3335 60.05352 22 7.795 128.016 1.1 10 15.2304 5.954725 1.6093 0.391 17.771 1.4181 43.038356 0.0003 43,44PIPR42S6.4718 76.06779 22 7.8 151.13 1.1 10 18.8376 7.413025 1.6093 0.391 32.179 1.7727 42.037464 0.0003 43,44PIPR43S6.4642 103.0919 22 7.805 166.624 1.1 10 25.05 10.2081 2.0691 0.391 60.036 1.7727 43.038356 0.0003 43,44PIPR44S7.0015 103.0919 22 7.78 163.83 1.1 10 32.064 4.010325 1.8392 0.391 58.115 1.7727 40.03568 0.0003 43,44PIPR45S5.9820 107.0954 22 7.79 157.48 1.1 10 18.2364 15.43368 1.6093 0.391 61.957 1.7727 43.038356 0.0003 43,44PIPR46S7.4331 134.1195 22 7.8 199.7075 1.1 10 32.2644 13.00318 1.6093 0.391 88.854 1.7727 43.038356 0.0003 43,44PIPR47S6.0725 45.04014 22 7.815 128.524 1.1 10 14.028 2.18745 1.3794 0.391 3.362 1.0636 41.036572 0.0003 43,44PIPR48S7.2713 46.04103 22 7.82 150.876 1.1 10 14.028 2.18745 6.2072 1.5639 5.7635 7.0906 42.037464 0.0003 43,44PIPR49S5.4175 45.04014 22 7.82 131.064 1.1 10 14.028 2.18745 15.173 1.5639 10.566 15.245 41.036572 0.0003 43,44PIPR50S6.2395 45.04014 22 7.81 160.2105 1.1 10 14.2284 2.18745 35.174 1.5639 21.613 36.162 41.036572 0.0003 43,44PIPR51S6.2194 44.03925 22 7.82 182.88 1.1 10 15.03 2.18745 62.992 1.5639 40.825 70.906 40.03568 0.0003 43,44PIPR52S4.9667 45.04014 22 7.81 180.848 1.1 10 14.4288 2.18745 101.39 1.9549 59.076 107.78 41.036572 0.0003 43,44PIPR53S6.1183 46.04103 22 7.81 176.784 1.1 10 14.2284 2.18745 57.015 19.158 40.825 71.97 42.037464 0.0003 43,44PIPR54S5.7931 189.1686 22 7.82 188.9125 1.1 10 55.11 15.79825 1.6093 0.782 152.25 1.0636 42.037464 0.0003 43,44PIPR55S5.2814 46.04103 22 7.865 125.603 1.1 10 14.6292 3.15965 1.3794 0.391 3.362 1.0636 42.037464 0.0003 43,44PIPR56S3.8765 75.0669 22 7.87 117.348 1.1 10 24.4488 5.954725 1.3794 0.391 30.739 1.0636 41.036572 0.0003 43,44PIPR57S3.7460 46.04103 22 7.865 114.554 1.1 10 14.4288 3.15965 19.771 0.391 12.488 18.436 41.036572 0.0003 43,44PIPR58S3.8963 74.06601 22 7.85 126.492 1.1 10 24.4488 6.07625 18.392 0.391 38.903 18.436 42.037464 0.0003 43,44PIPR59S5.1820 133.1186 22 7.85 172.72 1.1 10 41.082 11.6664 18.392 0.391 98.94 18.436 42.037464 0.0003 43,44PIPR60S5.0050 76.06779 22 7.85 167.3225 1.1 10 24.048 6.07625 47.589 0.782 58.115 52.116 43.038356 0.0003 43,44PIPR61S6.3379 134.1195 22 7.84 226.695 1.1 10 40.8816 11.6664 49.198 0.782 118.63 51.052 43.038356 0.0003 43,44PIPR62S6.5522 52.04638 22 7.96 84.201 0.3 10 12.024 4.13185 1.6093 0.391 10.566 1.7727 42.037464 0.0003 43,46PIPR63S7.7846 51.04549 22 7.96 97.79 0.3 10 11.2224 3.8888 2.7588 0.782 10.566 3.5453 41.036572 0.0003 43,46PIPR64S5.4254 50.0446 22 7.945 70.0786 0.3 10 11.022 3.767275 5.9773 1.5639 12.007 8.1542 41.036572 0.0003 43,46PIPR65S5.7632 51.04549 22 7.965 81.5848 0.3 10 11.2224 3.8888 11.955 2.3459 15.369 15.245 42.037464 0.0003 43,46PIPR66S5.0152 51.04549 22 7.96 77.4319 0.3 10 11.2224 3.767275 23.22 3.1279 21.613 30.135 41.036572 0.0003 43,46PIPR67S5.9195 53.04728 22 7.97 110.871 0.3 10 11.2224 3.767275 46.899 4.6918 33.62 59.207 41.537018 0.0003 43,46PIPR68S5.4017 53.04728 22 7.96 151.892 0.3 10 11.6232 3.8888 117.94 7.0377 68.201 141.81 42.037464 0.0003 43,46PIPR69S4.1225 52.04638 22 7.94 175.26 0.3 10 11.4228 3.767275 236.79 10.948 128.24 279.72 43.038356 0.0003 43,46PIPR70S6.6575 47.04192 25 7.82 145.288 1.1 10 13.9359 2.983276 1.6093 0.391 3.362 1.4181 42.53791 0.0003 43,44PIPR71S4.6725 47.04192 20 7.82 111.76 1.1 10 13.9359 2.983276 1.6093 0.391 3.362 1.4181 43.038356 0.0003 43,44E-8Appendix E. BLM TableModel OutputModel InputBLMData LabelCriticalAccumulationHardness(mg/L)Temp(oC) pHDissolvedLC50 (μg/L)DOC(mg/L)HumicAcid (%)Ca(mg/L)Mg(mg/L)Na(mg/L)K(mg/L)SO4(mg/L)Cl(mg/L)Alkalinity(mg/L)S(mg/L)NotesPIPR72S2.3613 47.04192 15 7.82 79.1845 1.1 10 13.9359 2.983276 1.6093 0.391 3.362 1.4181 42.53791 0.0003 43,44PIPR73S1.1782 47.04192 10 7.82 60.0075 1.1 10 13.9359 2.983276 1.6093 0.391 3.362 1.4181 42.53791 0.0003 43,44PIPR74S7.6860 140.1249 22 8.03 370.078 0.3 10 29.058 12.03098 25.059 4.3008 60.036 25.881 98.087416 0.0003 43,46PIPR75S10.9585 88.0785 22 7.965 292.1 0.3 10 19.038 7.04845 14.943 2.7369 37.943 17.017 63.056196 0.0003 43,46PIPR76S7.9470 59.05263 22 7.89 101.473 0.3 10 12.024 4.61795 9.1959 0.782 23.054 9.9268 39.034788 0.0003 43,46PIPR77S6.9448 41.03657 22 7.825 62.5094 0.3 10 8.2164 3.038125 7.5866 2.7369 13.928 6.3815 29.025868 0.0003 43,46PIPR78S5.9976 27.02408 22 7.745 42.0624 0.3 10 5.6112 1.822875 4.598 2.3459 8.6452 4.2544 23.020516 0.0003 43,46PIPR79S9.0570 43.03836 22 7.885 172.466 1.1 10 10.4208 2.67355 1.6093 0.782 2.8817 1.4181 42.037464 0.0003 43,44PIPR80S0.7034 25.0223 22 7.565 12.4333 0.3 10 6.68596 2.02764 3.4485 1.1729 4.3226 4.9634 16.014272 0.0003 43,46PIPR81S7.0672 107.0954 22 8.105 271.272 0.3 10 28.6924 8.631893 14.254 1.9549 19.212 16.308 80.07136 0.0003 43,46PIPR82S4.9660 87.0776 22 7.055 71.12 0.3 10 23.3293 7.018455 13.564 1.9549 19.212 15.954 58.051736 0.0003 43,46PIPR83S5.1028 85.07582 22 7.33 79.629 0.3 10 22.793 6.857111 13.794 1.9549 19.212 15.954 58.051736 0.0003 43,46PIPR84S5.4229 88.0785 22 7.605 99.53625 0.3 10 23.5975 7.099127 13.564 1.9549 19.212 15.954 59.052628 0.0003 43,46PIPR85S6.5439 87.0776 22 7.745 132.715 0.3 10 23.3293 7.018455 14.484 1.9549 18.731 15.954 59.052628 0.0003 43,46PIPR86S5.4310 87.0776 22 8.07 137.16 0.3 10 23.3293 7.018455 12.644 1.9549 18.731 15.954 59.052628 0.0003 43,46PIPR87S5.4306 87.0776 22 8.375 182.245 0.3 10 23.3293 7.018455 13.334 1.9549 18.731 15.954 59.052628 0.0003 43,46PIPR88S5.7955 87.0776 22 8.73 268.9225 0.3 10 23.3293 7.018455 14.254 1.9549 18.731 14.89 59.052628 0.0003 43,46PIPR89S6.9862 87.0776 22 8.115 188.976 0.3 10 23.3293 7.018455 12.874 1.9549 18.731 15.954 59.052628 0.0003 43,46PIPR90S8.5781 251.2239 22 7.2 662.559 0.3 10 67.127 20.35751 57.475 4.6918 72.524 62.397 150.1338 0.0003 43,46PIPR91S9.0461 252.2248 22 7.575 904.875 0.3 10 67.3945 20.43861 57.475 4.6918 70.603 62.043 164.14629 0.0003 43,46PIPR92S8.7054 252.2248 22 7.915 995.68 0.3 10 67.3945 20.43861 57.475 4.6918 73.484 62.043 150.1338 0.0003 43,46PIPR93S6.4404 251.2239 22 8.275 891.54 0.3 10 67.127 20.35751 57.475 4.6918 73.484 62.043 143.12756 0.0003 43,46PIPR94S8.4348 200.1784 22 8.05 757.6185 0.3 10 53.5426 16.18781 37.243 3.5188 49.47 46.798 128.11418 0.0003 43,46PIPR95S8.0730 140.1249 22 7.95 404.8125 0.3 10 37.4414 11.35479 22.99 2.3459 28.817 25.172 99.088308 0.0003 43,46PIPR96S8.8271 90.08028 22 8.045 262.128 0.3 10 24.1338 7.260471 14.254 1.9549 18.731 15.599 65.05798 0.0003 43,46PIPR97S2.3840 19.01695 22 7.525 20.447 0.3 10 5.08133 1.541007 3.4485 0.782 0.9606 4.9634 19.016948 0.0003 43,46PIPR98S2.6680 34.03033 22 7.53 23.1648 0.3 10 9.0929 2.757591 3.4485 0.782 9.6058 4.6089 20.01784 0.0003 43,46PIPR99S4.5268 51.04549 22 7.54 34.9885 0.3 10 13.6394 4.136386 3.4485 0.782 16.81 4.6089 21.018732 0.0003 43,46PIPR100S3.5167 29.02587 22 7.585 27.94 0.3 10 7.75571 2.352063 3.4485 0.782 5.2832 4.6089 22.019624 0.0003 43,46PIPR101S3.1703 30.02676 22 7.605 26.67 0.3 10 8.02315 2.433168 1.3794 0.782 4.3226 2.4817 23.020516 0.0003 43,46PIPR102S1.9033 27.02408 22 7.55 20.32 0.3 10 7.22084 2.189852 10.345 1.1729 5.2832 13.118 20.01784 0.0003 43,46PIPR103S2.9068 27.02408 22 7.525 26.67 0.3 10 7.22084 2.189852 20.691 1.5639 10.566 26.59 20.01784 0.0003 43,46PIPR104S6.9464 90.08028 22 7.995 182.88 0.3 10 24.1338 7.260471 14.254 1.9549 19.212 15.954 63.056196 0.0003 43,46PIPR105S4.3303 60.05352 22 8.11 96.6724 0.3 10 16.0463 4.866337 11.955 1.5639 3.8423 17.372 58.051736 0.0003 43,46E-9Appendix E. BLM TableModel OutputModel InputBLMData LabelCriticalAccumulationHardness(mg/L)Temp(oC) pHDissolvedLC50 (μg/L)DOC(mg/L)HumicAcid (%)Ca(mg/L)Mg(mg/L)Na(mg/L)K(mg/L)SO4(mg/L)Cl(mg/L)Alkalinity(mg/L)S(mg/L)NotesPIPR106S6.1231 120.107 22 8.09 182.88 0.3 10 32.0926 9.732674 11.955 1.5639 33.62 17.372 59.052628 0.0003 43,46PIPR107S 5.3380 180.1606 22 8.09 190.6905 0.3 10 48.1389 14.59901 11.955 1.5639 62.438 17.017 58.051736 0.0003 43,46PIPR108S 4.7175 91.08117 22 8.125 127.0635 0.3 10 24.3369 7.380611 11.955 1.5639 19.212 15.954 59.052628 0.0003 43,46PIPR109S 5.7327 90.08028 22 8.155 148.59 0.3 10 24.0695 7.299505 2.299 6.2557 15.85 6.027 60.05352 0.0003 43,46PIPR110S 6.5363 93.08296 22 8.135 223.52 0.3 10 24.8718 7.542822 35.864 3.9098 27.377 49.989 62.055304 0.0003 43,46PIPR111S 6.7795 92.08206 22 8.145 283.1465 0.3 10 24.6043 7.461717 71.728 7.4287 41.305 102.81 61.054412 0.0003 43,46PIPR112S 5.0174 91.08117 22 8.19 150.241 0.3 10 24.402 7.341142 14.484 15.248 18.731 17.372 62.055304 0.0003 43,46PIPR113S 6.2630 144.1284 22 8.38 644.525 0.3 10 38.5111 11.67921 34.485 3.1279 12.488 42.189 138.1231 0.0003 43,46PIPR114S 5.5141 292.2605 22 8.27 697.5475 0.3 10 78.092 23.68284 34.485 3.1279 87.893 57.079 137.1222 0.0003 43,46PIPR115S 5.1749 440.3925 22 8.225 752.475 0.3 10 117.673 35.68647 34.485 3.1279 175.31 41.125 133.11864 0.0003 43,46PIPR116S 5.8459 217.1936 22 8.31 653.415 0.3 10 58.0341 17.59992 34.485 3.1279 46.588 43.253 133.11864 0.0003 43,46PIPR117S 6.1591 218.1945 22 8.305 646.3665 0.3 10 58.3016 17.68102 6.8969 1.5639 38.903 9.5723 140.12488 0.0003 43,46PIPR118S 5.9250 212.1891 22 8.345 939.8 0.3 10 56.6969 17.19439 103.45 7.8197 65.319 124.79 143.12756 0.0003 43,46PIPR119S 8.2172 92.08206 22 8.125 253.365 0.3 10 24.6701 7.421814 14.254 1.9549 19.212 16.663 63.056196 0.0003 43,46PIPR120F 0.3052 48 25 8.03 109.44 2.64 10 14.1077 3.111984 1.35 0.57 3.54 1.25 44 0.0003 1,2,3,6,7,15,26PIPR121F 0.3617 45 25 8.04 116.16 2.64 10 13.2259 2.917485 1.27 0.57 3.33 1.17 44 0.0003 1,2,3,6,7,15,26PIPR122F 0.1755 46 25 7.98 84.96 2.64 10 13.5198 2.982318 1.3 0.57 3.4 1.2 41 0.0003 1,2,3,6,7,15,26PIPR123F 3.4889 30 25 6.82 418.56 10.4652 10 7.1362 2.964634 1.625 0.5 6.125 1.25 21 0.0003 1,2,3,6,7,27,28PIPR124F 1.8656 37 25 7.28 495.36 11.3373 10 8.80131 3.656382 2.0042 0.5 7.5542 1.5417 21 0.0003 1,2,3,6,7,27,28PIPR125F 2.8066 87 25 7.11 1522.56 31.3956 10 20.6978 8.4403 16.071 1.855 22.35 18.629 20 0.0003 1,2,3,6,7,27,29PIPR126F 3.1774 73 25 6.94 1083.84 24.4188 10 17.2174 7.3329 10.539 1.5232 18.439 13.619 18 0.0003 1,2,3,6,7,27,29PIPR127F 1.4538 84 25 7.07 528 14.5155 10 20.4644 8.008 6 1.4 34.5 10.95 12 0.0003 1,2,3,6,7,27,28PIPR128F 1.0075 66 25 6.97 960.96 32.9018 10 16.0792 6.292 4.7143 1.4 27.107 8.6036 12 0.0003 1,2,3,6,7,27,28PIPR129F 1.2809 43.9 25 7.4 88.32 2 10 12.9026 2.846168 1.24 0.57 3.24 1.14 42.4 0.0003 1,2,6,7,8,14,15PIPR130F 0.0860 47.04192 22 8.1 27.94 1.1 10 13.9359 2.983276 1.6093 0.391 3.362 1.4181 42.53791 0.0003 43,44PIPR131F 1.1899 243.2168 22 8.01 105.7275 1.1 10 92.7261 2.884195 47.129 0.391 3.362 143.23 43.038356 0.0003 43,44PIPR132F 0.1230 255.7279 22 8.01 40.0558 1.1 10 14.1661 53.5752 1.6093 0.391 3.362 143.23 43.538802 0.0003 43,44PIPR133F 0.4522 47.04192 22 8.1 64.262 1.1 10 13.9359 2.983276 47.589 0.391 3.362 72.324 43.538802 0.0003 43,44PIPR134F 0.3833 45.04014 22 8.02 49.01565 1.1 10 13.3428 2.856328 1.6093 0.391 3.362 1.4181 43.038356 0.0003 43,44PIPR135F 0.3216 45.04014 22 8.65 67.7164 1.1 10 13.3428 2.856328 1.6093 0.391 3.362 1.4181 47.041924 0.0003 43,44PIPR136F 0.1834 45.54059 22 7.3 18.669 1.1 10 13.4911 2.888065 1.6093 0.391 3.362 1.4181 44.039248 0.0003 43,44PIPR137F 0.1256 49.04371 22 6.63 6.1468 1.1 10 14.5289 3.110224 1.6093 0.391 3.362 1.4181 49.043708 0.0003 43,44PIPR138F 0.2961 45.04014 22 7.16 20.447 1.1 10 13.3428 2.856328 1.6093 0.391 3.362 15.599 26.023192 0.0003 43,44PIPR139F 2.8408 43.03836 22 7.93 93.36405 1.1 10 12.7498 2.72938 1.6093 0.391 3.362 1.4181 41.036572 0.0003 43,44E-10Appendix E. BLM TableModel OutputModel InputBLMData LabelCriticalAccumulationHardness(mg/L)Temp(oC) pHDissolvedLC50 (μg/L)DOC(mg/L)HumicAcid (%)Ca(mg/L)Mg(mg/L)Na(mg/L)K(mg/L)SO4(mg/L)Cl(mg/L)Alkalinity(mg/L)S(mg/L)NotesPIPR140F0.0373 45.54059 22 7.91 245.364 6.1 83.7705 13.4911 2.888065 1.6093 0.391 3.362 1.4181 44.039248 0.0003 43,47PIPR141F1.3667 45.04014 22 7.94 72.3392 1.1 10 13.3428 2.856328 1.6093 0.391 3.362 1.4181 43.038356 0.0003 43,44PIPR142F0.0310 45.04014 22 7.95 229.8065 6.1 83.7705 13.3428 2.856328 1.6093 0.391 3.362 1.4181 43.038356 0.0003 43,47PIPR143F0.1023 45.54059 22 7.94 195.453 3.6 72.5 13.4911 2.888065 1.6093 0.391 3.362 1.4181 44.039248 0.0003 43,47PIPR144F0.1038 45.04014 22 7.91 109.347 2.35 57.8723 13.3428 2.856328 1.6093 0.391 3.362 1.4181 42.037464 0.0003 43,47PIPR145F1.9076 44.03925 22 7.87 78.0034 1.1 10 13.0463 2.792854 1.6093 0.391 3.362 1.4181 42.037464 0.0003 43,44PIPR146F0.4905 44.03925 22 7.84 45.52315 1.1 10 13.0463 2.792854 1.6093 0.391 3.362 19.145 17.015164 0.0003 43,44PIPR147F1.3078 22.52007 22 6.01 4.3815 0.3 10 6.01736 1.824876 3.4485 0.391 3.362 4.2544 15.01338 0.0003 43,46PIPR148F1.5995 24.02141 22 7.02 12.4333 0.3 10 6.41852 1.946535 3.6784 0.391 3.362 4.9634 17.015164 0.0003 43,46PIPR149F2.4015 23.02052 22 8 26.8605 0.3 10 6.15108 1.865429 4.1382 0.782 3.362 4.9634 17.51561 0.0003 43,46PIPR150F2.3670 21.51918 22 9.01 51.3334 0.3 10 5.74992 1.743771 4.598 1.5639 3.362 4.9634 19.016948 0.0003 43,46PTLU01S4.0390 173 22 8.3 364.8 0.5 10 28.5511 24.739 53.583 4.282 166.08 3.8824 117 0.0003 1,2,3,4,6,7,20PTLU02S9.0637 173 22 7.25 460.8 0.5 10 28.5511 24.739 53.583 4.282 166.08 3.8824 117 0.0003 1,2,3,4,6,7,20PTOR01F0.2752 25 7.8 7.3 22.08 1.1 10 7.1535 1.9754 4.8154 0.7 3.997 5.9792 25 0.0003 1,2,3,6,7,9,10PTOR02F0.1587 54 11.5 7.3 17.28 1.1 10 15.0937 3.6371 6.8831 0.7 12.163 9.6854 43 0.0003 1,2,3,6,7,9,10XYTE01S2.6511 173 22 8.15 211.2 0.5 10 28.5511 24.739 53.583 4.282 166.08 3.8824 117 0.0003 1,2,3,4,6,7,20XYTE02S4.5011 173 22 8.05 326.4 0.5 10 28.5511 24.739 53.583 4.282 166.08 3.8824 117 0.0003 1,2,3,4,6,7,20POAC01S2.2126 167 22 8 153.6 0.5 10 27.5609 23.881 51.724 4.1335 160.32 3.7478 115 0.0003 1,2,3,4,6,7,20LEMA01R 25.6628 85 20.2 7.3 2200 1.1 10 23.9 6.5 0.64 0.46 4.32 1.5 82 0.0003 50LEMA02F 25.8381 45 20 7.5 1056 1.1 10 13.2259 2.917485 1.3 0.57 3.4 1.2 43 0.0003 1,2,3,6,7,8LEMA03F 27.6113 25.9 19 7.03 960 1.5 10 6.38814 2.42165 5.4743 1.6 26.489 19.425 27.1 0.0003 1,2,3,6,7,16LEMA04F 22.5658 85 21.85 7.45 1300 1.1 10 23.9 6.5 0.64 0.46 4.32 1.5 82 0.0003 50ETFL01S5.5744 170 20 7.8 316.8 0.5 10 27.9 24.2 52.5 4.2 163 3.80 115 0.0003 1,3,4,22ETFL02S5.7421 170 20 7.8 327.36 0.5 10 27.9 24.2 52.5 4.2 163 3.80 115 0.0003 1,3,4,22ETFL03S5.8278 170 20 7.9 358.08 0.5 10 27.9 24.2 52.5 4.2 163 3.80 115 0.0003 1,3,4,22ETFL04S6.4920 170 20 7.8 376.32 0.5 10 27.9 24.2 52.5 4.2 163 3.80 115 0.0003 1,3,4,22ETLE01S3.7314 167 22 8 249.6 0.5 10 27.5609 23.881 51.724 4.1335 160.32 3.7478 115 0.0003 1,2,3,4,6,7,20ETNI01S7.8536 170 20 7.8 473.28 0.5 10 27.9 24.2 52.5 4.2 163 3.80 115 0.0003 1,3,4,22ETNI02S7.7256 170 20 7.8 463.68 0.5 10 27.9 24.2 52.5 4.2 163 3.80 115 0.0003 1,3,4,22ETNI03S9.1617 170 20 7.8 577.92 0.5 10 27.9 24.2 52.5 4.2 163 3.80 115 0.0003 1,3,4,22ETNI04S8.5329 170 20 7.8 526.08 0.5 10 27.9 24.2 52.5 4.2 163 3.80 115 0.0003 1,3,4,22ETRU01S0.4735 167 22 8.2 57.6 0.5 10 27.5609 23.881 51.724 4.1335 160.32 3.7478 115 0.0003 1,2,3,4,6,7,20BUBO01S1.7185 167 22 7.9 115.2 0.5 10 27.5609 23.881 51.724 4.1335 160.32 3.7478 115 0.0003 1,2,3,4,6,7,20E-11

# Appendix F. Regression PlotsSpeciesStudyTest EndpointControl Value EC50StandardDeviationEC20 EC10Snail,Campeloma decisum (Test 1)Arthur and Leonard 1970 LC Survival 0.925 14.50 0.192 8.73 7.01Snail,Campeloma decisum (Test 2)Arthur and Leonard 1970 LC Survival 0.875 11.80 0.339 10.94 9.16Cladoceran,Daphnia pulexWinner 1985 LC Survival 1.00 4.57 0.260 2.83 2.24Cladoceran,Daphnia pulexWinner 1985 LC Survival 0.900 11.3 0.111 9.16 8.28Caddisfly,Clistoronia magnificaNebeker et al. 1984b LC Emergence (adult1st gen)0.750 20.0 0.300 7.67 5.63Bluegill (larval),Lepomis macrochirusBenoit 1975 ELS Survival 0.880 39.8 0.250 27.15 21.60Species Study Test EndpointControl Value EC50 Slope EC20 EC10Cladoceran,Ceriodaphnia dubiaCarlson et al. 1986 LC Reproduction 13.10 14.6 1.36 9.17 7.28Cladoceran,Daphnia magnaChapman et al. Manuscript LC Reproduction 171.5 16.6 1.40 12.58 10.63Cladoceran,Daphnia magnaChapman et al. Manuscript LC Reproduction 192.1 28.4 1.59 19.89 16.34Cladoceran,Daphnia magnaChapman et al. Manuscript LC Reproduction 88.0 15.8 1.00 6.06 3.64Rainbow trout,Oncorhynchus mykissSeim et al. 1984 ELS Biomass 137.6 40.7 1.69 27.77 22.16Rainbow trout,Oncorhynchus mykissBesser et al. 2001 ELS Biomass 1224 29.2 1.99 20.32 16.74Chinook salmon,Oncorhynchus tshawytschaChapman 1975, 1982 ELS Biomass 0.901 9.55 1.27 5.92 4.47Fathead minnow,Pimephales promelasLind et al. manuscript ELS Biomass 108.4 11.4 4.00 9.38 8.67Appendix F. Analyses of Chronic DataThe following pages contain figures and other information related to the regression and probability distribution analyses that were performed to calculatechronic EC20s. The initial parameter estimates are shown in the tables below. In the figures that follow, circles denote measured responses and solid linesdenote estimated regression lines.Final EstimatesFinal EstimatesProbability Distribution AnalysisLogistic Regression AnalysisF-1

### F -2 

# Evaluation of the Chronic Data Available for Freshwater Species

Following is a species-by-species discussion of each chronic test on copper evaluated for this document. Also presented are the results of regression analysis and probability distribution analysis of each dataset that was from an acceptable chronic test and contained sufficient acceptable data. For each such dataset, this appendix contains a figure that presents the data and regression/probability distribution

line.

Brachionus calyciflorus . The chronic toxicity of copper was ascertained in 4-day renewal tests conducted at regular intervals throughout the life of the freshwater rotifer, B. calyciflorus (Janssen et al. 1994). The goal of this study was to develop and examine the use of this rotifer as a viable test organism. The effect of copper on the age-specific survivorship and fertility of B. calyciflorus was determined, but no individual replicate data were provided and only three copper concentrations were tested, which precludes these data from further regression analysis. Chronic limits based on the intrinsic rate of natural increase were 2.5 μg/L total copper (NOAEC) and 5.0 μg/L total copper (LOAEC). The chronic value determined via traditional hypothesis testing is 3.54 μg/L total copper (Table 2a).

Campeloma decisum . Adult C. campeloma were exposed to five concentrations of total copper and a control (Lake Superior water) under flow-through conditions in two 6-week studies conducted by Arthur and Leonard (1970). Adult survival in the two separate chronic copper toxicity test trials was markedly reduced in the two highest copper concentrations, 14.8 and 28.0 μg/L, respectively. The authors reported that growth, as determined from cast exoskeleton, was not measurable for this test species, although the authors did observe that the adult snails would not consume food at the two highest copper concentrations. Control survival was 80 percent or greater. Chronic values of 10.88 μg/L total copper were obtained for survival based on the geometric mean of the NOAEC and LOAEC of 8.0 and 14.8 μg/L, respectively, in both tests. The corresponding EC20s were 8.73 and 10.94 μg/L (Table 2a).

Ceriodaphnia dubia. The chronic toxicity of copper to C. dubia was determined in ambient river water collected upstream of known point-source discharges of domestic and industrial wastes as part of a water effect ratio study (Carlson et al. 1986). In this study, survival and young production of C. dubia were assessed using a 7-day life-cycle test. Organisms were not affected at total copper concentrations ranging from 3 to 12 μg/L (5 to 10 μg/L dissolved copper). There was a 62.7 percent reduction in survival and 97 percent reduction in the mean number of young produced per female at 32 μg/L total copper (27 μg/L dissolved copper). No daphnids survived to produce young at 91 μg/L total copper. Control survival during the study was 80 percent, which included one male. The chronic value EC20

selected for C. dubia in this study, 9.17 μg/L derived from a nonlinear regression evaluation, was based on mean number of young produced (reproduction).

The effects of water hardness on the chronic toxicity of copper to C. dubia were assessed by Belanger et al. (1989) using 7-day life-cycle tests. C. dubia 2 to 8 hours old were exposed to copper in ambient surface water from the New and Clinch Rivers, Virginia. Mean water hardness levels were 179 and 94 mg/L as CaCO 3 

# , respectively. Test water was renewed on days 3 and 5. The corresponding chronic values for reproduction based on the NOAEC and LOAEC approach were 7.9 and <19.3 μg/L dissolved copper, respectively. The EC20 value for number of young (neonates) produced in Clinch River water (water hardness of 94 mg/L as CaCO 3 

# ) was 19.36 μg/L dissolved copper. The EC20 for young produced in New River water was not calculated. The chronic values were converted to total copper using the freshwater conversion factor for copper 0.96 (e.g., 7.897/0.96). The resulting total chronic values for the New and Clinch rivers are 8.23 and 20.17 μg/L, respectively.

### F -3



# Copper was one of 12 toxicants examined by Oris et al. (1991) in their comparisons between a 4- day survival and reproduction toxicity test utilizing C. dubia and a standard 7-day life-cycle test for the species. The reported 7-day chronic values for survival and reproduction (mean total young per living female) in two tests based on the traditional hypothesis testing techniques were 24.5 and 34.6 μg/L total copper. Comparable point estimates for these 7-day tests could not be calculated using regression analysis.

Daphnia magna . Blaylock et al. (1985) reported the average numbers of young produced for six

broods of D. magna

in a 14-day chronic exposure to copper. A significant reduction was observed in the mean number of young per female at a concentration of 30 μg/L total copper, the highest copper concentration tested. At this concentration, young were not produced at brood intervals 5 and 6. Reproduction was not affected at 10 μg/L total copper. The chronic value determined for this study (17.32 μg/L total copper) was based on the geometric mean of the NOAEC, 10 μg/L, and LOAEC, 30 μg/L.

Van Leeuwen et al. (1988) conducted a standard 21-day life-cycle test with D. magna . The water hardness was 225 mg/L as CaCO 3 

# . Carapace length was significantly reduced at 36.8 μg/L total copper, although survival was 100 percent at this concentration. Carapace length was not affected at 12.6 μg/L total copper. No daphnids survived at 110 μg/L concentration. The highest concentration not significantly different from the control for survival was 36.8 μg/L. The lowest concentration significantly different from the control based on survival was 110 μg/L, resulting in a chronic value of 63.6 μg/L for survival. The chronic value based on carapace length was 21.50 μg/L. The 21-day EC10 as reported by the author was 5.9 μg/L total copper.

Chronic (21-day) renewal toxicity tests were conducted using D. magna to determine the relationship between water hardness (nominal values of 50, 100, and 200 mg/L as CaCO 3 

# , respectively) and the toxicity of total copper (Chapman et al. unpublished manuscript). All test daphnids were <1 day old at the start of the tests. The dilution water was well water from the Western Fish Toxicology Station (WFTS), Corvallis, Oregon. Test endpoints were reproduction (total and live young produced per female) and adult survival. The survival of control animals was 100 percent at nominal water hardness levels of 50 and 200 mg/L as CaCO 3 

# , and 80 percent at a hardness of 100 mg/L as CaCO 3 

# . The chronic values for total young produced per female (fecundity) based on the geometric mean of the NOAEC and LOAEC were 13.63, 29.33, and 9.53 μg/L at the nominal hardness levels of 50, 100, and 200 mg/L as CaCO 3 

# , respectively. The corresponding EC20 values for reproduction calculated using nonlinear regression analysis were 12.58, 19.89, and 6.06 μg/L total copper. The chronic toxicity of copper to D. magna was somewhat ameliorated from an increase in water hardness from 50 to 100 mg/L as CaCO 3 

# , but slightly increased from 100 to 200 mg/L as CaCO 3 

# .

Daphnia pulex . Winner (1985) evaluated the effects of water hardness and humic acid on the chronic toxicity (42-day) of copper to D. pulex . Contrary to the expectation that sublethal endpoints are more sensitive indicators of chronic toxicity, reproduction was not a sensitive indicator of copper stress in this species. Water hardness also had little effect on the chronic toxicity of copper (similar to D.

magna trends), but humic acid significantly reduced chronic toxicity of copper when added to the varying water types. The survival chronic values based on the NOAEC and LOAEC values for the three low to no humic acid studies were 4.90, 7.07, and 12.25 μg/L total copper at hardnesses of 57.5, 115, and 230 (0.15 mg/L HA) μg/L as CaCO 3 

# , respectively. The EC20 values calculated for the low and high hardness studies using nonlinear regression techniques were 2.83 and 9.16 μg/L at hardness values of 57.5 and 230 (0.15 mg/L HA) μg/L as CaCO 3 

# , respectively.

### F -4



# Clistoronia magnifica . The effects of copper on the lifecycle of the caddisfly, C. magnifica , were examined in Nebeker et al. (1984b). The test included continuous exposure of first-generation aquatic larvae and pupae through to a third generation of larvae. A significant reduction in adult emergence occurred at 13.0 μg/L total copper from first-generation larvae. No observed adverse effect to adult emergence occurred at 8.3 μg/L total copper. Percent larval survival was close to the control value of 80 percent. The chronic value based on hypothesis testing was 10.39 μg/L total copper. The corresponding EC20 value for adult emergence was 7.67 μg/L total copper.

Oncorhynchus mykiss . The growth and survival of developing O. mykiss embryos continuously and intermittently exposed to copper for up to 85 days post-fertilization was examined by Seim et al. (1984). Results only from the continuous exposure study are considered here for deriving a chronic value. A flow-through apparatus was used to deliver six concentrations and a control (untreated well water; average of 3 μg/L copper) to a single incubation chamber. Continuous copper exposure ofsteelhead embryos in the incubation chambers was begun 6 days post-fertilization. At 7 weeks postfertilization, when all control fish had hatched and reached swim-up stage, subsamples of approximately 100 alevins were transferred to aquaria and the same exposure pattern continued. Dissolved oxygen remained near saturation throughout the study. Water hardness averaged 120 mg/L as CaCO 3 

# . Survival of steelhead embryos and alevins exposed continuously to total copper concentrations in the range of 3 (controls) to 30 μg/L was greater than 90 percent or greater. Survival was reduced at 57 μg/L and completely inhibited at 121 μg/L. A similar effect on survival was observed for embryos and alevins exposed to a mean of 51 (peak 263) and 109 (peak 465) μg/L of copper in the intermittent exposure, respectively. The adverse effect of continuous copper exposure on growth (measured on a dry weight basis) was observed at concentrations as low as 30 μg/L. (There was a 30 percent reduction in growth during the intermittent exposure at 16 μg/L.) The chronic limits for survival of embryos and alevin steelhead trout exposed continuously to copper were 16 and 31 μg/L, respectively (geometric mean = 22.27 μg/L). The EC20 for biomass for the continuous exposure was 27.77 μg/L.

Besser et al. (2001) conducted an ELS toxicity test with copper and the rainbow trout, O. mykiss

, starting with eyed embryos and continuing for 30 days after the fish reached the swim-up stage. The total test period was 58 days. The test was conducted in ASTM moderately hard reconstituted water with a hardness of approximately 160 to 180 mg/L as CaCO 3 

# . Twenty-five eyed embryos were held in each of four replicate egg cups at each concentration. Survival was monitored daily. At the end of the test, surviving fish in each replicate chamber were weighed (dry weight). Dry weights were used to determine growth and biomass of surviving fish. The no observed effect concentrations (NOECs) for survival and biomass were both 12 μg/L and the lowest observed effect concentrations (LOECs) for survival and biomass was also the same for both endpoints, 22 μg/L. The chronic values for biomass and survival based on the geometric mean of the NOEC and LOEC were 16.25 μg/L. The corresponding EC20 for biomass was 20.32 μg/L.

Oncorhynchus tshawytscha . The draft manuscript prepared by Chapman (1975/1982) provides the results from a 4-month egg through fry partial chronic test conducted to determine the effects of copper on survival and growth of O. tshawytscha . Continuous exposure occurred from several hours post-fertilization through hatch, swim-up, and feeding fry stages. The test was terminated after 14 weeks post-hatch. The dilution water was WFTS well water. Because of the influence of the nearby Willamette River on the hardness of this well water, reverse osmosis water was mixed periodically with ambient well water to attain a consistent hardness. The typical hardness of this well water was approximately 23 mg/L

as CaCO 3 

# . Control survival exceeded 90 percent for the test. The measured total copper concentrations during the test were 1.2 (control), 7.4, 9.4, 11.7, 15.5, and 20.2 μg/L, respectively. Copper adversely affected survival at 11.7 μg/L copper and higher, and growth was reduced at all copper concentrations tested compared with the growth of control fish. The chronic limits for copper in this study were

### F -5



# estimated to be less than 7.4 μg/L. The EC20 value estimated for biomass is 5.92 μg/L total copper based on a logistic nonlinear regression model.

Salmo trutta . McKim et al. (1978) examined the survival and growth (expressed as standing crop) of embryo-larval and early juvenile brown trout to copper. The most sensitive exposure was with embryos exposed for 72 days. The NOAEC and LOAEC, as obtained from the figure, were 20.8 and 43.8 μg/L total copper, respectively. Data were not available to calculate point estimates at the 20 percent effect level using regression analysis. The chronic value selected for this species was 29.91 μg/L total copper (geometric mean of 20.8 and 43.8 μg/L total copper).

Salvelinus fontinalis . Sauter et al. (1976) examined the effects of copper on selected freshwater fish species at different hardness levels (softwater at 37.5 mg/L as CaCO 3 

# ; hardwater at 187 mg/L as

CaCO 3 

# ) during a series of partial life-cycle (PLC) tests. The species tested were brook trout ( Salvelinus fontinalis ), channel catfish ( Ictalurus punctatus ), and walleye ( Stizostedion vitreum ). Because of the poor embryo and larval survival of control animals (in all cases less than 70 percent), results from tests with channel catfish and walleye were not included in Table 2a. One of the replicate control chambers from the PLC tests conducted with brook trout in hard water also exhibited poor hatchability (48 percent) and survival (58 percent) between 31 and 60 days of exposure. Therefore, the data for brook trout in hard water were not included in the subsequent EC20 (regression) analysis either.

The softwater test with brook trout was conducted using untreated well water with an average water hardness of 35 mg/L as CaCO 3 

# . This PLC exposure consisted of six copper concentrations and a control. Hatchability was determined by examining randomly selected groups of 100 eggs from each replicate exposure tank. Growth and survival of fry were determined by impartially reducing the total sample size to 50 fry per tank and assessing their progress over 30 day intervals up to 60 days post-hatch. The chronic limits based on the growth (wet weight and total length) of larval brook trout after 60 days of exposure to copper in soft water were <5 and 5 μg/L. The resultant chronic value for soft water based on hypothesis testing was <5 μg/L. The corresponding EC20 values based on total length, wet weight, and biomass (the product of wet weight and survival) for brook trout in the soft-water exposures after 60 days were not amenable to nonlinear regression analysis.

McKim et al. (1978) examined survival and growth (expressed as standing crop) of embryolarval and early juvenile brook trout exposed to copper. The embryo exposure was for 16 days, and the larval-early-juveniles exposure lasted 60 days. The NOAEC and LOAEC were 22.3 and 43.5 μg/L total copper, respectively. Data were not available to calculate point estimates at the 20 percent effect level using regression analysis. The chronic value for this species was 31.15 μg/L total copper (geometric mean of 22.3 and 43.5 μg/L total copper).

Salvelinus namaycush . McKim et al. (1978) examined the survival and growth (expressed as standing crop) of embryo-larval and early juvenile lake trout exposed to copper. The embryo exposure was for 27 days, and the larval-early-juveniles exposure lasted 66 days. The NOAEC and LOAEC were 22.0 and 43.5 μg/L total copper, respectively. Data were not available to calculate point estimates at the 20 percent effect level using regression analysis. The chronic value for this species was 30.94 μg/L total copper (geometric mean of 22.0 and 43.5 μg/L total copper).

Esox lucius . McKim et al. (1978) examined the survival and growth (expressed as standing crop) of embryo-larval and early juvenile northern pike exposed to copper. The embryo exposure was for 6 days, and the larval-early-juveniles exposure lasted 34 days. The NOAEC and LOAEC were 34.9 and 104.4 μg/L total copper, respectively. The authors attributed the higher tolerance of E. lucius to copper to the very short embryonic exposure period compared with salmonids and white sucker, Catostomus

### F -6



# commersoni. Data were not available to calculate point estimates at the 20 percent effect level using regression analysis. The chronic value for this species was 60.36 μg/L total copper (geometric mean of 34.9 and 104.4 μg/L total copper).

Pimephales notatus . An experimental design similar to that described by Mount and Stephan (1967) and Mount (1968) was used to examine the chronic effect of copper on the bluntnose minnow, P.

notatus (Horning and Neiheisel 1979). Measured total copper concentrations were 4.3 (control), 18.0, 29.9, 44.1, 71.8, and 119.4 μg/L, respectively. The experimental dilution water was a mixture of spring water and demineralized City of Cincinnati tap water. Dissolved oxygen was kept at 5.9 mg/L or greater throughout the test. Total water hardness ranged from 172 to 230 mg/L as CaCO 3 

# . The test was initiated with 22 6-week-old fry. The fish were later separated according to sex and thinned to a sex ratio of 5 males and 10 females per duplicated test chamber. Growth (total length) was significantly reduced in parental and first (F 1 

# ) generation P. notatus after 60 days of exposure to the highest concentration of copper tested (119.4 μg/L). Survival of parental P. notatus exposed to this same high test concentration was also lower (87 percent) at the end of the test compared with the other concentrations (range of 93 to 100 percent). Copper at concentrations of 18 μg/L and greater significantly reduced the number of eggs produced per female. The number of females available to reproduce was generally the same up to about 29.9 μg/L of copper. The chronic limits were based on an NOAEC and LOAEC of <18 and 18 μg/L for number of eggs produced per female. An EC20 was not estimated by nonlinear regression; nevertheless, in this case an EC20 is likely to be substantially below 18 : 

# g/L.

Pimephales promelas . The results from a 30-day ELS toxicity test to determine the chronic toxicity of copper to P. promelas using dilution water from Lake Superior (hardness ranging from 40 to 50 mg/L as CaCO 3 

# ) was included in Table 2a from a manuscript prepared by Lind et al. in 1978. In this experiment, five test concentrations and a control were supplied by a continuous-flow diluter. The exposure began with embryos 1 day post-fertilization. Pooled results from fish dosed in replicate exposure chambers were given for mean percentage embryo survival to hatch, mean percentage fish survival after hatch, and mean fish wet weight after 30 days. The percentage of embryo survival to hatch was not affected by total copper concentrations as high as 52.1 μg/L total copper. Survival after hatch, however, was compromised at 26.2 μg/L, and mean wet weight of juvenile fathead minnows was significantly reduced at 13.1 μg/L of copper. The estimated EC20 value for biomass was 9.376 μg/L total

copper.

Catastomus commersoni . McKim et al. (1978) examined the survival and growth (expressed as standing crop) of embryo-larval and early juvenile white sucker exposed to copper. The embryo exposure was for 13 days, and the larval-early-juvenile exposure lasted 27 days. The NOAEC and LOAEC were 12.9 and 33.8 μg/L total copper, respectively. The resulting chronic value based on hypothesis testing for this species was 20.88 μg/L total copper (geometric mean of 12.9 and 33.8 μg/L total copper).

Lepomis macrochirus . Results from a 22-month copper life-cycle toxicity test with bluegill ( L.

macrochirus ) were reported by Benoit (1975). The study included a 90-day embryo-larval survival and growth component. The tests were conducted at the U.S. EPA National Water Quality Laboratory in Duluth, Minnesota, using Lake Superior water as the dilution water (average water hardness = 45 mg/L

as CaCO 3 

# ). The test was initiated in December 1969 with 2-year-old juvenile L. macrochirus . In May 1971, the fish were sexed and randomly reduced to three males and seven females per tank. Spawning commenced on 10 June 1971. The 90-day embryo-larval exposure was initiated when 12 lots of 50 newly hatched larvae from one of the two control groups were randomly selected and transferred to duplicate grow-out chambers at 1 of 6 total copper concentrations: 3 (control), 12, 21, 40, 77, and 162 μg/L, respectively. In the 22-month juvenile through adult exposure, survival, growth, and reproduction were unaffected at 77 μg/L of copper and below. No spawning occurred at 162 μg/L. Embryo hatchability and

### F -7



# survival of 4-day-old larvae at 77 μg/L did not differ significantly from those of controls. However, after 90 days of exposure, survival of larval L. macrochirus at 40 and 77 μg/L was significantly lower than for controls, and no larvae survived at 162 μg/L. Growth remained unaffected at 77 μg/L. Based on the 90- day survival of bluegill larvae, the chronic limits were estimated to be 21 and 40 μg/L (geometric mean = 28.98 μg/L). The corresponding EC20 for embryo-larval survival was 27.15 μg/L.

### F- 8 

# Campeloma decisum (Test 1), Life-cycle, Arthur and Leonard 1970

Campeloma decisum (Test 2), Life-cycle, Arthur and Leonard 1970

Ceriodaphnia dubia (Clinch River), Life-cycle, Belanger et al. 1989 EC20 = 8.73 μg/L

EC20 = 10.94 μg/L

EC20 = 19.36 μg/L

### F -9 

# Lepomis macrochirus , Early Life-stage, Benoit 1975

Oncorhynchus mykiss , Early Life-Stage, Besser et al. 2001

Ceriodaphnia dubia , Life-cycle, Carlson et al. 1986

EC20 = 27.15 μg/L

EC20 = 20.32 μg/L

EC20 = 9.17 μg/L

### F -10 

# Daphnia magna (Hardness 104), Life-cycle, Chapman et al. Manuscript

Daphnia magna (Hardness 211), Life-cycle, Chapman et al. Manuscript

Daphnia magna (Hardness 51), Life-cycle, Chapman et al. Manuscript EC20 = 19.89 μg/L

EC20 = 6.06 μg/L

EC20 = 12.58 μg/L

### F -11 EC20 = 7.67 μg/L 

# Oncorhynchus tshawytscha , Early Life-Stage, Chapman 1975 & 1982

Pimephales promelas , Early Life-stage, Lind et al. 1978

Clistoronia magnifica , Life-cycle, Nebeker et al. 1984a EC20 = 5.92 μg/L

EC20 = 9.38 μg/L

### F -12 

# Oncorhynchus mykiss, Early Life-stage, Seim et al. 1984

Daphnia pulex (Hardness 230 HA 0.15), Life-cycle, Winner 1985

Daphnia pulex (Hardness 57) , Life-cycle, Winner 1985 EC20 = 27.77 μg/L

EC20 = 9.16 μg/L

EC20 = 2.83 μg/L

# Appendix G. Example Water Quality Criteria Values Using the BLM and the Hardness Equation

## Appendix G: Representative water quality criteria values using the BLM and the Hardness equation approaches for waters with a range in pH, Hardness, and DOC concentrations. The BLM calculation assumed that alkalinity was correlated with pH, and that other major ions were correlated with hardness based on observed correlations in EPA synthetic water recipes.

pH Hardness DOC Hardness Equation Based

Water Quality

Criterion for

Cu [1] 

## BLM Based

Instantaneous Water Quality

Criterion for Cu mg/L CaCO 3 

## mg / L μ g / L μ g / L

2 5.9 1.6

4 5.9 3.3

8 5.9 6.8

16 5.9 14.3

2 11.3 1.9

4 11.3 3.8

8 11.3 7.7

16 11.3 16.0

2 21.7 2.3

4 21.7 4.5

8 21.7 9.2

16 21.7 18.9

2 41.5 2.8

4 41.5 5.6

8 41.5 11.4

16 41.5 23.1

2 5.9 3.9

4 5.9 8.0

8 5.9 16.4

16 5.9 34.3

2 11.3 4.4

4 11.3 8.8

8 11.3 18.0

16 11.3 37.0

2 21.7 5.1

4 21.7 10.3

8 21.7 20.7

16 21.7 42.4

2 41.5 6.2

4 41.5 12.4

8 41.5 24.9

16 41.5 50.6 6.5 40

80

159

317

7.0 40

80

159

317

G-1

## pH Hardness DOC Hardness Equation Based

Water Quality

Criterion for

Cu [1] 

## BLM Based

Instantaneous Water Quality

Criterion for Cu mg/L CaCO 3 

## mg / L μ g / L μ g / L

2 5.9 7.9

4 5.9 15.8

8 5.9 32.4

16 5.9 67.3

2 11.3 8.7

4 11.3 17.4

8 11.3 35.3

16 11.3 72.5

2 21.7 10.1

4 21.7 20.1

8 21.7 40.5

16 21.7 82.4

2 41.5 12.0

4 41.5 23.9

8 41.5 47.8

16 41.5 96.8

2 5.9 13.8

4 5.9 27.6

8 5.9 55.8

16 5.9 115.0

2 11.3 15.5

4 11.3 30.6

8 11.3 61.4

16 11.3 125.1

2 21.7 18.0

4 21.7 35.3

8 21.7 70.3

16 21.7 142.0

2 41.5 21.5

4 41.5 41.6

8 41.5 82.3

16 41.5 165.1 7.5 40

80

159

317

8.0 40

80

159

317

G-2

## pH Hardness DOC Hardness Equation Based

Water Quality

Criterion for

Cu [1] 

## BLM Based

Instantaneous Water Quality

Criterion for Cu mg/L CaCO 3 

## mg / L μ g / L μ g / L

2 5.9 22.5

4 5.9 43.3

8 5.9 85.6

16 5.9 172.9

2 11.3 26.0

4 11.3 49.1

8 11.3 96.0

16 11.3 191.6

2 21.7 31.4

4 21.7 58.0

8 21.7 111.7

16 21.7 220.6

2 41.5 39.1

4 41.5 70.3

8 41.5 132.8

16 41.5 259.6

Notes:

[1] : Hardness Equation: CMC = e (0.9422 [ln(H)] - 1.7)



## where: H = water hardness (mg/L CaCO 3 

## ) 8.5 40

80

159

317

G-3

# Appendix H. Unused Data

### H -1 APPENDIX H . UNUSED DATA



# Based on the requirements set forth in the guidelines (Stephan et al. 1985), the following studies
are not acceptable for the following reasons and are classified as unused data.

Studies Were Conducted with Species That Are Not Resident in North America

Abalde et al. (1995)

Abel (1980)
Ahsanullah and Ying (1995)

Ahsanullah et al. (1981)
Aoyama and Okamura (1984)
Austen and McEvoy (1997)
Bougis (1965)

Cid et al. (1995, 1996a,b)

Collvin (1984)

Cosson and Martin (1981)
Daly et al. (1990a,b, 1992)

Denton and Burdon-Jones (1986)
Drbal et al. (1985)
Giudici and Migliore (1988)
Giudici et al. (1987, 1988)
Gopal and Devi (1991)
Gustavson and Wangberg (1995)
Hameed and Raj (1989)
Heslinga (1976)

Hori et al. (1996)
Huebner and Pynnonen (1992)

Ismail et al. (1990)
Jana and Bandyopadhyaya (1987)

Jindal and Verma (1989)

Jones (1997) Kadioglu and Ozbay (1995)

Karbe (1972)

Knauer et al. (1997)

Kulkarni (1983)
Kumar et al. (1985)

Lan and Chen (1991)

Lee and Xu (1984)

Luderitz and Nicklisch (1989)
Majori and Petronio (1973)
Masuda and Boyd (1993)
Mathew and Fernandez (1992)

Maund et al. (1992)
Migliore and Giudici (1988)

Mishra and Srivastava (1980)
Negilski et al. (1981)
Nell and Chvojka (1992)

Neuhoff (1983)

Nias et al. (1993)

Nonnotte et al. (1993)

Pant et al. (1980)
Paulij et al. (1990)

Peterson et al. (1996)
Pistocchi et al. (1997)
Pynnonen (1995) Raj and Hameed (1991)
Rajkumar and Das (1991)

Reeve et al. (1977)

Ruiz et al. (1994, 1996)
Saward et al. (1975)

Schafer et al. (1993)

Smith et al. (1993)
Solbe and Cooper (1976)

Steeman-Nielsen and Bruun-Laursen

(1976)
Stephenson (1983)

Takamura et al. (1989)
Taylor et al. (1991, 1994)

Timmermans (1992)
Timmermans et al. (1992)

Vardia et al. (1988)
Verriopoulos and MoraitouApostolopoulou (1982)

Visviki and Rachlin (1991)

Weeks and Rainbow (1991)

White and Rainbow (1982)
Wong and Chang (1991)
Wong et al. (1993)



# Copper Was a Component of a Drilling Mud, Effluent, Mixture, Sediment, or Sludge

Buckler et al. (1987)
Buckley (1994)

Clements et al. (1988)

de March (1988)

Hollis et al. (1996)
Horne and Dunson (1995)
Hutchinson and Sprague (1987) Kraak et al. (1993 and 1994a,b)

Lowe (1988)
McNaught (1989)

Munkittrick and Dixon (1987)
Pellegrini et al. (1993)
Roch and McCarter (1984a,b) Roch et al. (1986)
Sayer et al. (1991b)

Weis and Weis (1993)

Widdows and Johnson (1988)
Wong et al. (1982)

### H -2 

# These Reviews Only Contain Data That Have Been Published Elsewhere

Ankley et al. (1993)
Borgmann and Ralph (1984)
Chapman et al. (1968)

Chen et al. (1997)

Christensen et al. (1983)

Dierickx and Brendael-Rozen (1996)

DiToro et al. (1991)
Eisler (1981)

Eisler et al. (1979)

Enserink et al. (1991) Felts and Heath (1984)

Gledhill et al. (1997)
Handy (1996)
Hickey et al. (1991)

Janssen et al. (1994)

LeBlanc (1984)

Lilius et al. (1994)
Meyer et al. (1987)

Ozoh (1992c) Peterson et al. (1996)
Phillips and Russo (1978)
Phipps et al. (1995)
Spear and Pierce (1979b)

Starodub et al. (1987b)
Taylor et al. (1996)
Thompson et al. (1972)
Toussaint et al. (1995)



# No Interpretable Concentration, Time, Response Data, or Examined Only a Single Concentration

Asztalos et al. (1990)

Beaumont et al. (1995a,b)
Beckman and Zaugg (1988)
Bjerselius et al. (1993)
Carballo et al. (1995)

Daoust et al. (1984)
De Boeck et al. (1995b, 1997)

Dick and Dixon (1985)
Felts and Heath (1984)

Ferreira (1978)

Ferreira et al. (1979)

Hansen et al. (1993, 1996)

Heath (1987, 1991)
Hughes and Nemcsok (1988)

Julliard et al. (1996) Koltes (1985)
Kosalwat and Knight (1987)

Kuwabara (1986)

Lauren and McDonald (1985)
Leland (1983)

Lett et al. (1976)
Miller and McKay (1982)
Mis and Bigaj (1997)
Nalewajko et al. (1997)

Nemcsok et al. (1991)

Ozoh (1990)

Ozoh and Jacobson (1979)
Parrott and Sprague (1993)
Pyatt and Dodd (1986)

Riches et al. (1996) Sayer (1991)
Sayer et al. (1991a,b)

Schleuter et al. (1995, 1997)

Starcevic and Zielinski (1997)
Steele (1989)
Taylor and Wilson (1994)
Viale and Calamari (1984)

Visviki and Rachlin (1994b)
Waiwood (1980)

Webster and Gadd (1996)
Wilson and Taylor (1993a,b)
Winberg et al. (1992)

Wundram et al. (1996)

Wurts and Perschbacher (1994)



# No Useable Data on Copper Toxicity or Bioconcentration

Cowgill et al. (1986)

de March (1979)

Lehman and Mills (1994)
Lustigman (1986) Lustigman et al. (1985)

MacFarlane et al. (1986)

van Hoof et al. (1994)

Weeks and Rainbow (1992) Wong et al. (1977)

Wren and McCarroll (1990)

Zamuda et al. (1985)



# Results Not Interpretable as Total or Dissolved Copper

Brand et al. (1986)

MacFie et al. (1994)

Riedel (1983)

Sanders and Jenkins (1984) Sanders and Martin (1994)

Sanders et al. (1995)
Stearns and Sharp (1994)

Stoecker et al. (1986) Sunda et al. (1987)
Winberg et al. (1992)



# Some of these studies would be valuable if copper criteria were developed on the basis of cupric
ion activity.

### H -3



# Organisms Were Selected, Adapted or Acclimated for Increased Resistance to Copper

Fisher (1981)

Fisher and Fabris (1982)

Hall (1980)

Hall et al. (1989)

Harrison and Lam (1983)

Harrison et al. (1983)

Lumoa et al. (1983)

Lumsden and Florence (1983) Munkittrick and Dixon (1989)
Myint and Tyler (1982)

Neuhoff (1983)

Parker (1984)
Phelps et al. (1983)
Ray et al. (1981)

Sander (1982)

Scarfe et al. (1982) Schmidt (1978a,b)

Sheffrin et al. (1984)

Steele (1983b)

Takamura et al. (1989)
Viarengo et al. (1981a,b)

Wood (1983)



# Either the Materials, Methods, Measurements or Results Were Insufficiently Described

Abbe (1982)
Alam and Maughan (1995)
Balasubrahmanyam et al. (1987)
Baudouin and Scoppa (1974)
Belanager et al. (1991)
Benedeczky et al. (1991)

Benedetti et al. (1989)

Benhra et al. (1997)
Bouquegneau and Martoja (1982)

Burton and Stemmer (1990)

Burton et al. (1992)
Cabejszek and Stasiak (1960)

Cain and Luoma (1990)
Chapman (1975, 1982)

Cochrane et al. (1991)

Devi et al. (1991)
Dirilgen and Inel (1994)
Dodge and Theis (1979)
Doucet and Maly (1990)

Dunbar et al. (1993)
Durkina and Evtushenko (1991)

Enesco et al. (1989)
Erickson et al. (1997)

Evans (1980)
Ferrando and Andreu (1993)
Finlayson and Ashuckian (1979)
Furmanska (1979) Gibbs et al. (1981)

Gordon et al. (1980)
Gould et al. (1986)
Govindarajan et al. (1993)
Hayes et al. (1996)

Howard and Brown (1983)

Janssen et al. (1993)

Janssen and Persoone (1993)

Kean et al. (1985)

Kentouri et al. (1993)

Kessler (1986)
Khangarot et al. (1987)
Kobayashi (1996)

Kulkarni (1983)

Labat et al. (1977)

Lakatos et al. (1993)
LeBlanc (1985)

Leland et al. (1988)
Mackey (1983)
Magni (1994)
Martin et al. (1984)

Martincic et al. (1984)
McIntosh and Kevern (1974)
McKnight (1980)
Moore and Winner (1989)

Muramoto (1980, 1982)
Nyholm and Damgaard (1990) Peterson et al. (1996)
Pophan and D’Auria (1981)
Reed-Judkins et al. (1997)

Rehwoldt et al. (1973)

Riches et al. (1996)
Sakaguchi et al. (1977)

Sanders et al. (1995)
Sayer (1991)
Schultheis et al. (1997)

See et al. (1974)

Shcherban (1977)

Smith et al. (1981)
Sorvari and Sillanpaa (1996)
Stearns and Sharp (1994)
Strong and Luoma (1981)

Sullivan and Ritacco (1988)
Taylor (1978)
Taylor et al. (1994)
Thompson (1997)

Trucco et al. (1991)
Verma et al. (1980)

Visviki and Rachlin (1994a)
Watling (1983)

Winner et al. (1990)
Young and Harvey (1988, 1989)

Zhokhov (1986)

### H -4 

# Questionable Effect Levels Due to Graphical Presentation of Results

Alliot and Frenet-Piron (1990)

Andrew (1976)

Arsenault et al. (1993)
Balasubrahmanyam et al. (1987)
Bjerselius et al. (1993)

Bodar et al. (1989)

Chen (1994)
Cowgill and Milazzo (1991b)

Cvetkovic et al. (1991)

Dodoo et al. (1992)

Francisco et al. (1996) Gupta et al. (1985)

Hansen et al. (1996)
Hoare and Davenport (1994)

Lauren and McDonald (1985)
Llanten and Greppin (1993)

Metaxas and Lewis (1991)

Michnowicz and Weeks (1984)
Miersch et al. (1997)

Nasu et al. (1988)

Pearlmutter and Lembi (1986) Pekkala and Koopman (1987)

Peterson et al. (1984)

Romanenko and Yevtushenko (1985)

Sanders et al. (1994)

Smith and Heath (1979)

Stokes and Hutchinson (1976)

Winner and Gauss (1986)
Wong (1989)
Young and Lisk (1972)



# Studies of Copper Complexation With No Useable Toxicology Data for Surface Waters

Borgmann (1981)
Filbin and Hough (1979)
Frey et al. (1978)
Gillespie and Vaccaro (1978)
Guy and Kean (1980) Jennett et al. (1982)
Maloney and Palmer (1956)
Nakajima et al. (1979)
Stauber and Florence (1987)

Sunda and Lewis (1978) Swallow et al. (1978)
van den Berg et al. (1979)
Wagemann and Barica (1979)



# Questionable Treatment of Test Organisms or Inappropriate Test Conditions or Methodology

Arambasic et al. (1995)

Benhra et al. (1997)

Billard and Roubaud (1985)

Bitton et al. (1995)

Brand et al. (1986)
Bringmann and Kuhn (1982)
Brkovic-Popovic and Popovic

(1977a,b)
Dirilgen and Inel (1994)

Folsom et al. (1986)

Foster et al. (1994)

Gavis et al. (1981)

Guanzon et al. (1994)

Hawkins and Griffith (1982)

Ho and Zubkoff (1982) Hockett and Mount (1996)

Huebert et al. (1993)

Huilsom (1983)

Jezierska and Slominska (1997)
Kapu and Schaeffer (1991)

Kessler (1986)
Khangarot and Ray (1987a)
Khangarot et al. (1987)

Lee and Xu (1984)

Marek et al. (1991)

McLeese (1974)

Mis et al. (1995)

Moore and Winner (1989)

Nasu et al. (1988) Ozoh and Jones (1990b)

Reed and Moffat (1983)

Rueter et al. (1981)
Sayer et al. (1989)

Schenck (1984)
Shaner and Knight (1985)

Sullivan et al. (1983)

Tomasik et al. (1995)
Watling (1981, 1982, 1983)

Wikfors and Ukeles (1982)

Wilson (1972)
Wong and Chang (1991)
Wong (1992)



# High control mortalities occurred in all except one test reported by Sauter et al. (1976). Control
mortality exceeded 10% in one test by Mount and Norberg (1984). Pilgaard et al. (1994) studied
interactions of copper and hypoxia, but failed to run a hypoxic control. Beaumont et al. (1995a,b) studied
interactions of temperature, acid pH and copper, but never separated pH and copper effects. The 96-hour
values reported by Buikema et al. (1974a,b) were subject to error because of possible reproductive

interactions (Buikema et al. 1977).

### H -5 

# Bioconcentration Studies Not Conducted Long Enough, Not Steady-State,
Not Flow-through, or Water Concentrations Not Adequately Characterized or Measured

Anderson and Spear (1980a)

Felton et al. (1994)

Griffin et al. (1997)

Harrison et al. (1988)
Krantzberg (1989) Martincic et al. (1992)

McConnell and Harrel (1995)

Miller et al. (1992)

Ozoh (1994)
Wright and Zamuda (1987) Xiaorong et al. (1997)

Yan et al. (1989)
Young and Harvey (1988, 1989)

Zia and Alikhan (1989)



# Anderson (1994), Anderson et al. (1994), Viarengo et al. (1993), and Zaroogian et al. (1992)
reported on in vitro exposure effects. Benedeczky et al. (1991) studied only effects of injected copper.
Ferrando et al. (1993b) studied population effects of copper and cladoceran predator on the rotifer prey,
but the data are difficult to interpret. A similar problem complicated use of the cladoceran competition
study of LeBlanc (1985).