Monsanto Company CR240-13E3 Page 1 of 166 

Title 

Amended Application for an Experimental Use Permit to Test MON 87411, Combined Trait 

Insect Control Corn, Combinations Necessary for their Production, and Controls; (EPA File 

Symbol 524-EUP-104). 

Test Guideline 

Not applicable for this EUP application 

Data Requirement 

40 CFR Part 172 

 Authors 

M. Groth 

H. Kannall 

C. Patino 

Submission Date 

October 3, 2013 

Report Generated By 

Monsanto Company 

Registrant Submitting 

Monsanto Company 

800 North Lindbergh Blvd. 

St. Louis, MO 63167 

Submission ID 

CR240-13E3

Page Count 

1 of 166 

Volume 1 

 
The text below applies only to use of the data by the United States Environmental Protection 

Agency (U.S. EPA) in connection with the provisions of the Federal Insecticide, Fungicide 

and Rodenticide act (FlFRA). 

CLAIM OF CONFIDENTIALITY 

Information claimed as confidential on the basis of its falling within the scope ofFIFRA 

section 10( d)(l )(A), (B), or (C) has been removed to a confidential appendix, and is cited by 

cross-reference ~~be~t"dy submission. 

Submitter: ~d (":' ( Date: October 3. 2013 

Name: Mark E. Groth. B.A. 

Company: Monsanto Company 

We submit this material to the U.S. EPA specifically under the requirements set forth in 

FIFRA as amended, and consent to the use and disclosure of this material by the EPA strictly 

in accordance with FIFRA. By submitting this material to the EPA in accordance with the 

method and format requirements contained in PR Notice 2011-3, we reserve and do not waive 

any rights involving this material that are or can be claimed by the company notwithstanding 

this submission to the EPA. 

In the United States, the above statement supersedes all other statements of confidentiality 

that may occur elsewhere in this document. 

THESE DATA MAY BE CONSIDERED CONFIDENTIAL IN COUNTRIES 

OUTSIDE THE UNITED STATES. 

Monsanto Company CR240-13E3 Page 2 of 166 
GLP Compliance Statement 

This Administrative Volume does not meet the U.S. EPA Good Laboratory Practice 

requirements as specified in 40 CFR Part 160. This Experimental Use Permit Administrative 

Volume does not comprise a study under 40 CFR § 160.3. 

S'b""'M~/ Mark E. Groth, B.A. ~~ Date: 

Monsanto Company 

Study Director: 

0e'e 

~7 . Bookout, ~L M.S. 

Date: 

Monsanto Company 

Monsanto Company CR240-13E3 Page 3 of 166 


OPP Identifier 

&EPA United States Nnmber ENVIRONMENTAL PROTECTION AGENCY 

Washington, DC 20460 524-EUP-104 

Office of Pesticide Programs (7505C) 

Application for Experimental Use Permit to Ship and 

Use a Pesticide for Experimental Pnrposes Only 

1. Type of Application 2. Briefly explain (attach a separate sheet if necessary) 

D 0' Amendment (See No.2) 

Groth, M., Kannall, H., Patino, C. 2013. Amended Application for an 

New Experimental Use Permit to Test MON 87411, Combined Trait Insect 

D Control corn, Combinations Necessary for their Production, and 

Extension (Give Permit Number be/ow) Controls; (EPA File Symbol 524-EUP-104). 

Permit Number 524-EUP-I04 

3. Name and address of Firm/Person to Whom the Experimental Use 4. Name and address of Shipper only if shipment is intended or if 

Permit is to be Issued (include ZIP Code) (Type or Print) different from applicant's name and address (include Zip Code) 

Monsanto Company (Type or Print) 

800 NOlih Lindbergh Blvd. 

SI. Louis, MO 63167 

EPA Company Number 524 6. Is product registered with EPA? 

5. Name of Product: Corn RootwormwProtected, Glyphosale- 0 No Tolerant Com MON 87411, and Lepidopteran and Corn RootwonnD Protected, Glyphosate- and Glufosinate-Tolerant Corn stack Yes (Give Registration Number of File Symbol below) 

combinations 

Registration Number File Symbol 

7. Total Quantity of Product Proposed for ShipmenUUse 8. Acreage or Area to be Treated 9. Proposed Period of ShipmenUUse 

Pounds of formulated product 1,497,820 lbs seed 7,776 non-PIP 

4.8lbsCrylF 13.61bs Cry3Bbl 20,361 nonregistered PIP 

5.7 lbs CrylA.I05 61.71bsCry34Abl 11,508 registered PIP February 15,2014-

2.6 Ibs Cry2Ab2 1.51bs Cry35AhJ 6877 border acres February 28, 2016 

22.5 Ibs Vip3Aa20 46,522 TOTAL ACRES 

1.1 x IO·5lbsDvSnt7dsRNA 

Pounds of active ingredient 

10. Places from which shipped 11. CropsfSite to be Treated 

Monsanto Company, St. Louis, MO; Seed Companies and Licensees Corn a 

12. Specify the name and number of the contact person most familiar 13'7)Y,0:r?PA~untative with this application. 

Mark E. Groth 14. Title Regulatory Affairs 15. Date Signed 

Phone: (314) 694-3369 Manager October 3, 2013 

Certification 

This is to certify that food and feed derived from the experimental program will not be used or offered for consumption or sale for consumption, 

except by laboratory or experimental animals, if illegal residues are present in or on such food or feed. 

I certify that the statements I have made on this form and all attachments thereto are true, accurate, and complete. I acknowledge that any 

knowingly false or misleading statement may be punishable by fine or imprisonment, or both, under applicable law. 

Below fOI"EPA Use Only .' ". , ...... ". '. ,. ..... .', . . .. . 

In any correspondence 'on this applicaUon, refer to this number .•.. ReceIv~d by: EPA~OPP Registration 

. '.... . .•........ . .' Division. Washington. DC 20,460 

Normal review time, indicates 'that'processi'ng 'of-this application'shc)ljld be cbmpi~ted by (date) .. , '.' .' . . ". ... .' .,' 

Nanie of EPA Contact Person .... T.el~phone Number 

...... .' ... , " . . ' . . 

Monsanto Company CR240-13E3 Page 6 of 166 
Monsanto Company CR240-13E3 Pages 7 - 117 of 166

EPA Form 8540-4 Confidential Statement of Formula 

Information claimed confidential has been removed to the confidential attachment. See Cross 

Reference 1. 
&EPA UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

401 M Street, S. W. 

WASHINGTON, D.C. 20460 

Papcrworl" Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 1.25 hours per response 

for registration and 0.25 hours per response for reregistration and special review activities, including time for reading the instructions and 

completing the necessary forms, Send comments regarding burden estimate or any other aspect of this collection of information, including 

suggestions for reducing the burden 10 Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency, 401 M 

Street, S. W., Washington DC, 20460. Do not send the completed form to this address. 

Certification with Respect to Citation of Data 

Applicant's/Registratant's Name, Address, and Telephone Number: EPA Registration Number I File Symbol: 

Monsanto Company, 800 N0l1h Lindbergh Blvd., St. Louis, MO 63167 524-EUP-104 

Tel. (314) 694-3369 

Active Ingredient)s) andlor representative test compound(s): DvSnf7 dsRNA Date: 

and the genetic material (vector PV-ZMIR10871) neccssaty for its production in corn; October 3,2013 Bacillus tlllll'ingiensis CryIA.IOS, Cl'y2Ab2, CrylF, Cry3Bbl, Cry34Abl, Cry35Abl, 

Vip3Aa20 and the genetic material (vectors PV-ZNfIR245, PI-IP8999, PV-ZMIR39, 

PHP17622, pNOV1300), neceSSaIY for their production in corn. 

General Use Pattern(s) (list all those claimed for this product using 40 Product Name: 

CFR Part 158: Corn Rootwonn-Protected, Glyphosate-'l'olerant Corn, 

Terrestrial field crop MON 87411, and Lepidopteran and Corn RootwormProtected, Glyphosate- and Glufosinate-Tolerant Corn 

stack combinations 

NOTE: Ifyoul' product is a 100% repackaging of another purchased EPA-registered product labeled for all the same uses on 

your label, you do not need to submit this form. You must submit the Formulator's Exemption Statement (EPA Form 8570-27). 

D 1 am responding to a Data-Call-in Notice, and have included with this form a list of companies sent offers of compensation 

(the Data Matrix form should be used for this purpose). 

Section I: METHOD OF DATA SUPPORT (Check one method only) 

D I am using the cite-all method of support, and have included with ~ I am using the selective method of support (or cite-aU option 

this form a list of companies set offers of compensation (the under the selective method), and have included with this form a 

Data Matrix Form should be used for this purpose). completed list of data requirements (the Data Matrix form must 

be used). 

Section II: GENERAL OFFER TO PAY 

D [Required if using the cite-all method or when using the cite-all option under the selective method to satisfy one or more data requirements] 

I herebv off< '" aoree to Dav .10 , . with reoard 10 the loflhis . 10 Ihe exlenl reouired bv 

Section III: CERTIFICATION 

I certify that this application for registration, this form for reregistration, or this Data-Call-In response is supported by all data submitted or cited 

in the application for registration, the form for registration, or the Data-Call-In response. In addition, if the cite-all option or cite-all option under the 

selective method is indicated in Section 1, this application is supported by all data in the Agency's files that (1) concern the properties or effects of this 

product or an identical or substantially similar product, one or more of the ingredients in this product; and (2) is a type of data that would be required to 

be submitted under the data requirements in effect on the date of approval of this application if the application sought the initial registration of a product 

of identical or similar composition and uses. 

~ I certify that for each exclusive use study cited in support of this registration or reregistration, that I am the original data submitter or that I have 

obtained the written permission of the original data submitter to cite that study. 

I certify that for each study cited in support of this registration or reregistration that is not an exclusive use study, either: (a) I am the original data 

submitter; (b) I have obtained the permission of the original data submitter to use the study in support of this application; (c) all periods of eligibifity for 

compensation have expired for the study; (d) the study is in the public literature; (e) I have notified in writing the company that submitted the study and 

have offered (i) to pay compensation to the extent required by sections 3(c)(1)(F) andlor 3(c)(2)(8) of FIFRA; and (ii) to commence negotiations to 

determine the amount and terms of compensation, if any, to be paid for the use of the study. 

I certify that in all instances where an offer of compensation is required, copies of all offers to pay compensation and evidence of their delivery in 

accordance with sections 3(c)(1)(F) andlor 3(c)(2)(8) of FIFRA are available and will be submitted to the Agency upon request. Should I fail to produce 

such evidence to the Agency upon request. I understand that the Agency may initiate action to deny, cancel or suspend the registration of my product in 

conformity with FIFRA. 

I certify that the statements I have ma~~~is form and all attachments to It are true, accurate, and complete. I acknowledge that 

any knowinaiy.false of misl~;¥ting s!i!~ment 9:p.unishable b fine or imprisonment of both under the applicable law. 

Signature~ !/«~ Date Typed or Printed Name and Title 

Mark E. Groth 

October 3, 2013 Regulatory Affairs Manager 

Monsanto Company CR240-13E3 Page 118 of 166 
Monsanto Company CR240-13E3 Page 119 of 166 

Section A. Product Description

Monsanto Company is requesting an EUP to test a new PIP technology MON 87411, 

combined trait insect control corn, combinations necessary for their production, and 

controls. 

Development and testing of new PIPs and PIP combinations providing multiple modes of 

action against CRW and Lepidopteran corn pests is an important component of producing 

highly efficacious products that also support durable IRM. The PIP events comprising 

the combined trait products in this EUP include five registered corn events (MON 89034, 

EPA Reg. No. 524-576; TC1507, EPA Reg. No. 68467-2; MIR162, EPA Reg. No. 

67979-14; MON 88017, EPA Reg. No. 524-551; and DAS-59122-7, EPA Reg. No. 

68467-5), and one corn events representing a new class of PIP active against CRW spp. 

(MON 87411). 

Using biotechnology methods, Monsanto Company has developed MON 87411, that 

confer resistance to corn rootworm (CRW; Diabrotica spp.). MON 87411 contains a 

suppression cassette with an inverted repeat sequence (DvSnf7) derived from Diabrotica 

virgifera. The expression of the DvSnf7 suppression cassette results in the formation of a 

double stranded RNA (dsRNA) transcript. Upon consumption by the CRW pest, the 

plant-produced DvSnf7 dsRNA in MON 87411 result in the down-regulation of the 

targeted Snf7 gene and eventual CRW mortality. MON 87411 also produce the Cry3Bb1 

protein to protect against CRW larval feeding. In addition, MON 87411 are Roundup 

Ready corn events that produce the 5-enolpyruvylshikimate-3-phosphate synthase 

protein from Agrobacterium sp. strain CP4 (CP4 EPSPS), which confers tolerance to 

glyphosate, the active ingredient in Roundup agricultural herbicides. 

All of the above registered corn PIP products, produce active ingredients derived from 

Bacillus thuringiensis (B.t.). Crystal proteins derived from B.t. have been safely used in 

biotechnology-derived crops for over a decade. EPA has reviewed and registered the 

Cry1A.105, Cry2Ab2, Cry1F, Vip3Aa20, Cry3Bb1, and Cry34Ab1/Cry35Ab1 proteins 

as produced in corn and/or cotton. The environmental human health safety of these 

registered PIPs and proteins has been demonstrated and they are exempted from the 

requirement of a tolerance (see Section C on toxicity data within this submission). The 

DvSnf7 insecticidal nucleic acid component in MON 87411 are currently being evaluated 

for safety and efficacy. Nucleic acids are considered to be “generally recognized as safe” 

(GRAS) by the U.S. Food and Drug Administration (FDA) (1992) and exempt from the 

requirement of a tolerance under the Federal Food Drug and Cosmetic Act by the U.S. 

Environmental Protection Agency (EPA). 

 

 Roundup and Roundup Ready are registered trademarks of Monsanto Technology LLC. 
Monsanto Company CR240-13E3 Page 120 of 166 

Section B. Proposed Experimental Use Permit Label 

The attached master label for testing under the experimental use permit contains 

the following: 

 a proposed label for MON 89034 x TC1507 x MIR162 x MON 88017 x MON 

87411 x DAS-59122-7 (vector PV-ZMIR245, vector PHI8999, vector 

pNOV1300, vector PV-ZMIR39, vector PV-ZMIR10871, vector PHP17662) 

This proposed label contains the highest order stack including all single PIP events 

included within this EUP. Smaller order stacks or intermediates may also be shipped 

under this EUP as per the following Master Label. 
Monsanto Company CR240-13E3 Page 121 of 166 

MASTER LABEL 

EPA EXPERIMENTAL USE PERMIT NUMBER 524-EUP-104 

Plant-Incorporated Protectant Label

Keep Out Of Reach Of Children

CAUTION 

This Master Label for testing under the Experimental Use Permit (EUP) contains 

the following 46 materials. This Master Label will accompany each shipment of 

viable plant material required for trials conducted at the release site, in accordance 

with the EUP. The highest order stack is listed including every PIP material 

included within the EUP. All lower order stacks are covered under this Master 

Label: 

1. Lepidopteran-Protected Corn: MON 89034. 

2. Lepidopteran-Protected, Glufosinate-Tolerant Corn: TC1507. 

3. Lepidopteran-Protected Corn: MIR162. 

4. Corn rootworm-Protected, Glyphosate-Tolerant Corn: MON 88017. 

5. Corn rootworm-Protected, Glyphosate-Tolerant Corn: MON 87411. 

6. Corn rootworm-Protected, Glufosinate-Tolerant Corn: Cry34/35Ab1 

(DAS-59122-7). 

7. Lepidopteran-Protected, Glufosinate-Tolerant Corn: MON 89034 × TC1507. 

8. Lepidopteran-Protected Corn: MON 89034 × MIR162. 

9. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate-Tolerant 

Corn: MON 89034 × MON 88017. 

10. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate-Tolerant 

Corn: MON 89034 × MON 87411. 

11. Lepidopteran-Protected, Corn rootworm-Protected, Glufosinate-Tolerant 

Corn: MON 89034 × DAS-59122-7. 

12. Lepidopteran-Protected, Glufosinate-Tolerant Corn: MON 89034 × TC1507 × 

MIR162. 

FOR EXPERIMENTAL USE ONLY 

Not for sale to or use by any person other than a participant or cooperator of the EPAapproved Experimental Use Program. 

For use only at a site of a participant or cooperator and in accordance with the terms 

and conditions of the Experimental Use Permit. 

This labeling must be in possession of the user at the time of planting. 

For use in States (Alabama, Arkansas, California, Colorado, Florida, Georgia, Hawaii, 

Idaho, Illinois, Indiana, Iowa, Kansas, Kentucky, Louisiana, Michigan, Minnesota, 

Mississippi, Missouri, Nebraska, North Carolina, North Dakota, Ohio, Oklahoma, 

Pennsylvania, South Carolina, South Dakota, Tennessee, Texas, Washington, 

Wisconsin) and the Commonwealth of Puerto Rico.
Monsanto Company CR240-13E3 Page 122 of 166 

13. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: MON 89034 × TC1507 × MON 88017. 

14. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: MON 89034 × TC1507 × MON 87411. 

15. Lepidopteran-Protected, Corn rootworm-Protected, Glufosinate-Tolerant 

Corn: MON 89034 × TC1507 × DAS-59122-7. 

16. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate-Tolerant 

Corn: MON 89034 × MIR162 × MON 88017. 

17. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate-Tolerant 

Corn: MON 89034 × MIR162 × MON 87411. 

18. Lepidopteran-Protected, Corn rootworm-Protected, Glufosinate-Tolerant 

Corn: MON 89034 × MIR162 × DAS-59122-7. 

19. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: MON 89034 × MON 88017 × DAS-59122-7. 

20. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: MON 89034 × TC1507 × MIR162 × MON 87411. 

21. Lepidopteran-Protected, Corn rootworm-Protected, Glufosinate-Tolerant 

Corn: MON 89034 × TC1507 × MIR162 × DAS-59122-7. 

22. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: MON 89034 × TC1507 × MON 88017 × 

DAS-59122-7. 

23. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate-Tolerant 

Corn: MON 89034 × MON 88017 × MON 87411. 

24. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: MON 89034 × MON 87411 × DAS-59122-7. 

25. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: MON 89034 × TC1507 × MON 87411 × 

DAS-59122-7. 

26. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: MON 89034 × MIR162 × MON 88017 × 

DAS-59122-7. 

27. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: MON 89034 × MIR162 × MON 87411 × 

DAS-59122-7. 

28. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: MON 89034 × TC1507 × MIR162 × MON 88017 

× DAS-59122-7. 

29. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: MON 89034 × TC1507 × MIR162 × MON 87411 

× DAS-59122-7. 

30. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: MON 89034 × TC1507 × MON 88017 × MON 

87411 × DAS-59122-7. 

31. Lepidopteran-Protected, Glufosinate-Tolerant Corn: TC1507 × MIR162. 

32. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: TC1507 × MON 88017. 

33. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: TC1507 × MON 87411. 
Monsanto Company CR240-13E3 Page 123 of 166 

34. Lepidopteran-Protected, Corn rootworm-Protected, Glufosinate-Tolerant 

Corn: TC1507 × DAS-59122-7. 

35. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: TC1507 × MIR162 × MON 87411. 

36. Lepidopteran-Protected, Corn rootworm-Protected, Glufosinate-Tolerant 

Corn: TC1507 × MIR162 × DAS-59122-7. 

37. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: TC1507 × MON 88017 × DAS-59122-7. 

38. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: TC1507 × MON 87411 × DAS-59122-7. 

39. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: TC1507 × MIR162 × MON 87411 × 

DAS-59122-7. 

40. Lepidopteran-Protected, Corn rootworm-Protected, Glufosinate-Tolerant 

Corn: MIR162 × DAS-59122-7. 

41. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate- and 

Glufosinate-Tolerant Corn: MIR162 × MON 87411 × DAS-59122-7. 

42. Lepidopteran-Protected, Corn rootworm-Protected, Glyphosate-Tolerant 

Corn: MIR162 × MON 87411. 

43. Corn rootworm-Protected, Glyphosate-Tolerant Corn: MON 88017 × 

MON 87411. 

44. Corn rootworm-Protected, Glyphosate and Glufosinate-Tolerant Corn: 

MON 88017 × MON 87411 × DAS-59122-7. 

45. Corn rootworm-Protected, Glyphosate- and Glufosinate-Tolerant Corn: 

MON 88017 × DAS-59122-7. 

46. Corn rootworm-Protected, Glyphosate- and Glufosinate-Tolerant Corn: 

MON 87411 × DAS-59122-7. 
Monsanto Company CR240-13E3 Page 124 of 166 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of this Experimental Use Permit 

(EUP) is a violation of Federal law. The contents may only be used according to the 

approved EUP program. Cooperators and Participants must have at least one copy of the 

protocols in Section G prior to initiating any research with the contents. 

Test plots must not be located within 1⁄4 of a mile from the habitats of 

endangered/threatened lepidopteran and coleopteran species as listed by the US Fish and 

Wildlife Service. 

STORAGE AND DISPOSAL

Do not contaminate water, food, or feed by storage and disposal. 

Storage 

Store in a secure, cool, and dry place inaccessible to children. 

Seed and Plant Disposal 

Any seed and/or plant material not used in these experiments must be returned to 

Monsanto Company or seed company partners/affiliates, or disposed of as specified in 

the field protocols in Section G. 

Container Disposal 

Do not reuse bag. Ensure that the bag is completely empty of seed before disposal. 

Dispose as specified in Section G. 

 
Monsanto Company CR240-13E3 Page 125 of 166 

Monsanto EUP Shipment Summary Label 

524-EUP-104 

Event (See pages 1-3 for list of 

events) Net contents (lbs) 

 

 
Monsanto Company CR240-13E3 Page 126 of 166 

Lepidopteran-Protected, Coleopteran-Protected, GlyphosateTolerant, Glufosinate-Tolerant Corn: MON 89034 × TC1507 × 

MIR162 × MON 88017 × MON 87411 × DAS-59122-7 

This package contains corn seed producing any of the following insecticidal proteins 

from Bacillus thuringiensis, and/or a double stranded RNA (dsRNA) transcript 

comprising an inverted repeat sequence (DvSnf7) derived from Diabrotica virgifera. 

These proteins and/or dsRNA are effective in controlling feeding damage caused by 

lepidopteran and/or coleopteran insect pests. 

Active Ingredients: 

Bacillus thuringiensis Cry1A.105 protein and the genetic material necessary for its 

production (vector PV-ZMIR245) in corn event MON 89034 (OECD Unique Identifier: 

MON-89Ø34-3) ................................................................................................. ≤ 0.0056%* 

 

Bacillus thuringiensis Cry2Ab2 protein and the genetic material necessary for its 

production (vector PV-ZMIR245) in corn event MON 89304 (OECD Unique Identifier: 

MON-89Ø34-3) ................................................................................................. ≤ 0.0057%* 

Bacillus thuringiensis Cry1F protein and the genetic material necessary for its production 

(construct PHP8999; event TC1507) in corn ....................................... <0.0010 – 0.235%** 

Bacillus thuringiensis Vip3Aa20 insecticidal protein and the genetic material necessary 

for its production (via elements of vector pNOV1300) in corn event MIR162 (OECD 

Unique Identifier SYN-IR162-4) ..................................................................... ≤ 0.00751%* 

Bacillus thuringiensis Cry3Bb1 protein and the genetic material necessary for its 

production (vector PV-ZMIR39 and/or PV-ZMIR10871) in corn event MON 88017 

(OECD Unique Identifier MON-88Ø17-3) and/or MON 87411 ....................... ≤ 0.0070%* 

dsRNA transcript comprising a DvSnf7 inverted repeat sequence derived from 

Diabrotica virgifera, and the genetic material necessary for its production (vector 

PV-ZMIR10871) in corn event MON 87411............................................ ≤0.000000344%* 

Bacillus thuringiensis Cry34Ab1 insecticidal crystal protein and the genetic 

material necessary for its production (construct PHP17662) in event 

DAS-59122-7 corn ................................................................................ 0.000867-0.0088%* 

Bacillus thuringiensis Cry35Ab1 insecticidal crystal protein and the genetic 

material necessary for its production (construct PHP17662) in event 

DAS-59122-7 corn .............................................................................. 0.000413-0.00181%* 
Monsanto Company CR240-13E3 Page 127 of 166 

Other Ingredients: 

CP4 EPSPS protein (5-enolpyruvylshikimate-3-phosphate synthase) and the genetic 

material necessary for its production (vectors PV-ZMIR39 and PV-ZMIR10871) in corn 

events MON 88017 and MON 87411 (OECD Unique Identifier MON-88Ø17-3 and 

MON 87411) ....................................................................................................... ≤0.0069%* 

Substance produced by a marker gene and its controlling sequences in corn events 

TC1507 and DAS-59122-7 (PHP8999 and PS149B1) ..................................... <0.0020%** 

A marker protein and the genetic material necessary for its production (via elements of 

vector pNOV1300) in corn event MIR162 (OECD Unique Identifier 

SYN-IR162-4).................................................................................................. ≤ 0.00073%* 

*Percentage (wt/wt) on a dry weight basis whole plant (forage). 

**% total protein on a dry wt. basis as expressed in corn plant cells 

(whole plant). 

CAUTION Keep Out Of Reach Of Children 
Monsanto Company CR240-13E3 Page 128 of 166 

Section G. Proposed Experimental Program 

G.1. Justification of the acreage requested 

Monsanto Company is requesting an amended EUP (524-EUP-104) to test a new PIP 

technology, MON 87411, along with other combined trait insect control corn. This is an 

essential part of our commitment to develop and test new PIPs and combined-trait PIP 

products to provide multiple modes of action against corn rootworm (CRW) and/or 

Lepidopteran corn pests. This amended EUP program is a key component to developing 

highly efficacious and durable PIP products to meet diverse U.S. corn growers’ needs and 

support multiple-dose IRM strategies (‘pyramid’) to delay development of insect 

resistance to PIP products. 

Under this program, we are requesting a total of 46,522 EUP acres in aggregate across 

two years for MON 87411, other single- and combined-event corn PIP products, controls, 

and border corn. Of the 8,319 EUP acres requested for 2014, approximately 41%

represent unregistered PIPs for evaluation (primarily PIP combinations and potential 

breeding intermediates), and 12,043 EUP acres requested in 2015, approximately 46% 

represent unregistered PIPs. The balance of the remaining acres is comprised of 

registered PIP materials, border acres, and non-PIP materials that must be accounted for 

under the total EUP acres. 

As outlined further in Section G below, the number of acres for unregistered PIPs is 

distributed among various candidate PIP combinations. 

Protocol 1 is for inbred and hybrid breeding and observation nurseries. This involves 

seed development and increase for future testing as well as nursery observations of the 

experimental insect-protected corn traits in various genetic backgrounds. This involves 

activities such as introgression of biotech corn events into elite inbred corn lines; 

production of research hybrid seed for further research; field data on agronomics in the 

presence of these traits; and collection of samples and tissues for future analysis. This 

entire program involves 7,219 acres of unregistered PIPs among twenty-six test products 

over two years constituting ≤ 7.3% of the total requested acres. 

Protocol 2 involves development and collection of product characterization data 

including Phenotypic and Agronomic Observations, product Efficacy and Yield Benefits, 

Characterization, Performance, and Regulatory data. This entire program involves 

13,142 acres of unregistered PIPs among twenty-six test products over two years, 

constituting 28% of the total requested acres. 

The necessity for this program is driven by a number of important factors. Testing a 

broad set of trait combinations (ideally with locally-adapted germplasm) is necessary to 

understand and evaluate potential product suitability and efficacy, breeding 

combinability, PIP and IRM value, and the individual value each trait and/or event brings 

to a particular event combination. A key consideration is the need to offer growers 

biotech traits in combinations needed to meet local insect and weed-control needs while 

simultaneously meeting the IRM conditions required for the individual products. 

Achieving this objective requires multiple pyramided traits for IRM durability, value, and 
Monsanto Company CR240-13E3 Page 129 of 166 

appropriate spectrum of insecticidal activity, and must be evaluated under field 

conditions. Preparation and evaluation of biotech corn products using this approach takes 

many years in advance in order to meet and exceed future needs. A diverse portfolio of 

potential options is needed in this amended EUP program to develop data preparing 

breeding stock seed for future testing needs, inbred and hybrid breeding combinability, 

and data for a future registration request such as efficacy, expression data, and IRM 

value. 

Additionally, as with any PIP EUP program, a significant portion of the acres are 

requested or required as controls in studies and evaluations (e.g. registered PIPs such as 

MON 89034 or MON 88017). Registered PIP, border, and non-PIP acres all contribute 

to the total and account for over 56% of the 46,522 requested trial acreage total across 

two years. 

Finally, trial locations are not typically determined until the spring of any given year. 

Many factors such as weather may affect the trial locations. Acreage has been requested 

in consideration of such conditions. While we have projected our testing plans for 2014-

2015, it is imperative that we have flexibility to make adjustments when needed. Each 

season’s new data enables the researcher to anticipate needs regarding the following year. 

As such, we anticipate certain combinations of events may need larger trials in the second 

year over the first year. In order to meet these objectives when filing an amended EUP 

request a year in advance, the proposed acreage accounts for contingencies in our trial 

plans while requesting realistic numbers in the event the data demonstrate significant 

value. 

As appropriate, any inbred seed and/or hybrid seed, grain, or other plant material 

generated under this amended EUP will be used for future testing, planting, or will be 

destroyed. This is outlined in greater detail Section G.3.g. under Final Disposition. 

Monsanto certifies that none of the plantings under this amended EUP or any of its 

relevant protocols is solely for commercial increase. Hybrid seed (the type of seed that 

could be classified as “commercial seed”) produced under any of the amended EUP 

protocols will not be offered or made available for commercial sale, but will be used for 

future experiments, experimental planting, and/or evaluation. All hybrid seed and grain 

will ultimately be destroyed if not stored for necessary regulatory archival purposes. 

Inbred seed (breeding seed) that is produced under the amended EUP protocols will be 

evaluated, used to produce hybrid seed for experiments and evaluation, or destroyed, 

retained for future evaluation, development, and/or future breeding use. None of the 

seed, grain, or other plant material will be allowed to enter commerce for food or feed 

purposes. 

 
Monsanto Company CR240-13E3 Pages 130-132 of 166 

G.1.a Participants in the Experimental Program 

All Participants are research scientists employed by Monsanto Company. 

Participants Supervising the Experimental Program 

Information claimed confidential has been removed to the confidential attachment. 

See Cross Reference 2. 
Monsanto Company CR240-13E3 Page 133 of 166 

G.1.b Cooperators in the Experimental Program 

The names, addresses, and telephone numbers of Cooperators involved in the 

experimental work conducted under this EUP will be provided to the Agency prior to 

planting. 
Monsanto Company CR240-13E3 Page 134 of 166 

G.1.c Counties Where Experimental Trials will be Conducted 

Site selection for EUP trials is not typically decided until early spring of any given year. 

Many factors can affect the site selection process and new locations may be added 

throughout the duration of the EUP. 

Monsanto will conduct an analysis of selected sites. Monsanto will not plant 

experimental trials within 1,320 feet of reported locations of threatened or endangered 

lepidopteran and coleopteran species (excluding extirpated or historic locations). Species 

presence information (county-level) will be obtained from the FIFRA Endangered 

Species Task Force (FESTF) Information Management System (IMS). Prior to planting, 

species locations will be analyzed on the County and/or Sub-County level, using the 

following sources: 

1. U.S. Fish and Wildlife Services (USFWS) Data assembled by FESTF IMS; and 

2. FESTF Multi-Jurisdictional Database (also known as “NatureServe location 

data”) 

 
Monsanto Company CR240-13E3 Page 135 of 166 

G.2. Acreage, Seed and Active Ingredient Quantities by State or Possession 

Monsanto has projected the acreage needs for the field testing program for 2014-2015. 

The requested Experimental Use Permit contains a total of 33 un-registered PIP and 13 

registered PIP single and/or combined-trait products, non-PIP, and border materials. 

Table 1 lists the EUP test materials per un-registered and registered PIP subgroups. Each 

individual event listed is registered by the EPA except MON 87411. 

Table 1. EUP Test Materials per PIP subgroup 

Un‐Registered PIPs

MON 89034 x MIR162 TC1507 x MIR162

MON 89034 x MON 87411 TC 1507 x MON 88017

MON 89034 X DAS‐59122‐7 TC1507 x MON 87411

MON 89034 x TC1507 x MIR162 TC1507 x MIR162 X MON87411

MON 89034 x TC1507 x MON 87411 TC1507 X MIR162 X DAS‐59122‐7

MON 89034 x MIR162 x MON 88017 TC1507 X MON88017 x DAS‐59122‐7  

MON 89034 x MIR162 x MON 87411 TC1507 x MON 87411 x DAS‐59122‐7  

MON 89034 X MIR162 X DAS‐59122‐7 TC1507 X MIR162 X MON87411 X DAS‐59122‐7  

MON 89034 x TC1507 x MIR162 x MON 87411 MIR162 X DAS‐59122‐7

MON 89034 X TC1507 X MIR162 X DAS‐59122‐7 MIR162 X MON87411 x DAS‐59122‐7

MON 89034 X MON 88017 X MON 87411 MIR162 x MON 87411

MON 89034 X MON 87411 X DAS‐59122‐7   MON 88017 x MON 87411

MON 89034 x TC1507 x MON 87411 x DAS‐59122‐7   MON 88017 X MON 87411 X DAS‐59122‐7  

MON 89034 x MIR162 x MON 88017 x DAS‐59122‐7 MON 87411

MON 89034 X MIR162 X MON 87411 X DAS‐59122‐7   MON 87411 x DAS‐59122‐7

MON 89034 x TC1507 x MIR162 x MON 88017 x DAS‐59122‐7

MON 89034 x TC1507 x MIR162 x MON 87411 x DAS‐59122‐7  

MON 89034 x TC1507 x MON 88017 x MON 87411 x DAS‐59122‐7  

 
Monsanto Company CR240-13E3 Page 136 of 166 

Table 1 (continued). EUP Test Materials per PIP subgroup 

Registered PIPs

MON 89034

MON 89034 x TC1507

MON 89034 x MON 88017

MON 89034 x TC1507 x MON 88017

MON 89034 x TC1507 x DAS‐59122‐7

MON 89034 x MON 88017 x DAS‐59122‐7

MON 89034 x TC1507 x MON 88017 x DAS‐59122‐7

TC1507

TC1507 x DAS‐59122‐7

MIR162

MON 88017

MON 88017 x DAS‐59122‐7

DAS‐59122‐7

A total of 45,522 acres are requested for this EUP, which includes 31,869 acres of PIP 

materials, 7,776 acres of non-PIP materials, and 6,877 acres of border materials. The 

quantity of acres being requested for 2014-2015 is required to conduct field trials, using 

an extensive number of single and combined-trait products, and generate data necessary 

to register a combined-trait product under FIFRA. 

 
Monsanto Company CR240-13E3 Page 137 of 166 

Table 2 lists the acres per year and EUP test material type. 

Table 2. Acreage Distribution Among PIP, Non-PIP and Border Acres Over the 

Duration of the EUP 

Material

Type

2014 2015 Total

Acres Acres % Acres %

PIP 13558 66% 18311 70% 31869

Non‐PIP 3797 19% 3979 15% 7776

Border 3161 15% 3716 14% 6877

Total 20516 100% 26006 100% 46522

Table 3 lists the acres by experimental protocol and by EUP test material type. 

Table 3. Acreage Distribution Among PIP, Non-PIP and Border Acres By Protocol 

   Protocol 1

(Breeding and associated data) Protocol 2 (Characterization) Total Acres  (P1 + P2)

PIP 10615 21254 31869

Non‐PIP 2147 5630 7776

Border 2177 4700 6877

Total 14939 31584 46522

 
Monsanto Company CR240-13E3 Page 138 of 166 

Table 4 provides the acreage per state for 2014-2015. 

Table 4. Proposed Total Trial Acreage by State for 2014-2015 

State

Acres

PIP Non‐PIP Border Total

AL 384 34 36 454

AR 383 50 38 471

CA 32 23 8 63

CO 682 39 46 767

FL 190 10 10 210

GA 215 28 22 265

HI 5707 1333 1222 8262

IA 4276 967 856 6099

ID 2 1 1 4

IL 4100 990 857 5947

IN 1154 103 164 1421

KS 1434 598 330 2362

KY 200 14 16 230

LA 196 14 16 226

MI 385 62 95 542

MN 1854 148 322 2324

MO 564 406 319 1289

MS 705 310 231 1246

NC 188 19 14 221

ND 184 26 52 262

NE 2180 733 565 3478

OH 479 67 120 666

OK 24 18 10 52

PA 19 20 8 47

PR 2700 943 735 4378

SC 180 10 10 200

SD 1292 335 302 1929

TN 634 300 218 1152

TX 161 41 28 230

WA 353 59 94 506

WI 1018 76 132 1226

Total 31869 7776 6877 46522
Monsanto Company CR240-13E3 Page 139 of 166 

Tables 5-6 provide the proposed acreage for 2014-2015 per PIP combination and state for 

Protocol 1 and Protocol 2, respectively. The protocols are listed by number code as 

follows: 

Protocol 1: Inbred and Hybrid Breeding and Observation Nurseries 

Protocol 2: Product Characterization 

The acreage for PIP test materials includes acreage for each individual PIP test material. 

The acreage for non-PIP materials includes acreage for any non-PIP breeding materials 

and any non-intentional recipients of PIP test materials within the experimental trial. The 

acreage for border materials includes acreage intentionally planted as border adjacent to 

trial (if applicable). 

The allocation of these acres between corn materials and protocols represents best 

estimates as of this date. Weather events, pest infestation levels, and other factors may 

necessitate a reallocation of acres between protocols and traits. 

Table 7 provides the amount of seed (in pounds) expected to be planted in states over the 

duration of the EUP. 

Table 7 provides the amount of active ingredient per state to be used in the EUP program. 

Calculation of active ingredient for each protein is based on an expression level of the 

protein(s) in corn seed on a dry weight basis and/or DvSnf7 dsRNA. The calculations to 

determine the amount of active ingredient (lbs) in each PIP test material was performed 

using an estimated planting density of 75,000 kernels (~47 lbs.) per acre. 

Table 8 provides the pounds of seed used to calculate the active ingredient in Table 7.
Monsanto Company CR240-13E3 Page 140 of 166 

Table 5. Proposed Acreage for 2013-2014 per State/Territory for P1–Inbred and Hybrid Breeding an

Material 

AL 

AR 

CA 

CO 

GA 

HI 

IA 

ID 

IL 

IN 

KS 

Registered PIPs 

DAS-59122-7 0 0 0 0 0 246 26 0 26 0 0 MIR162 0 0 2 0 0 246 26 0 26 0 0 MON 88017 0 0 0 0 0 106 16 0 16 0 0 MON 88017 × DAS-59122-7 0 0 0 0 0 136 16 0 16 0 0 MON 89034 0 0 0 0 0 266 26 0 26 0 0 MON 89034 × MON 88017 0 0 4 0 0 86 16 0 16 0 0 MON 89034 × MON 88017 × DAS-59122-7 0 0 0 0 0 26 13 0 13 0 0 MON 89034 × TC1507 0 0 0 0 0 236 21 0 21 0 0 MON 89034 × TC1507 × DAS-59122-7 0 0 0 0 0 56 13 0 13 0 0 MON 89034 × TC1507 × MON 88017 0 0 0 0 0 56 16 0 16 0 0 MON 89034 × TC1507 × MON 88017 × DAS59122-7 0 0 0 0 0 86 16 0 16 0 0 TC1507 0 0 0 0 0 246 26 0 26 0 0 TC1507 × DAS-59122-7 0 0 0 0 0 86 16 0 16 0 0 Registered PIPs Total 0 0 6 0 0 1878 244 0 244 0 0 
Monsanto Company CR240-13E3 Page 141 of 166 

Table 5 (continued). Proposed Acreage for 2013-2014 per State/Territory for P1–Inbred and Hybrid BreeNurseries 

Material 

MO 

MS 

NC 

ND 

NE 

OH 

OK 

PA 

PR 

SC 

SD 

TN 

Registered PIPs 

DAS-59122-7 2 0 0 0 9 0 0 0 66 0 0 0 MIR162 2 0 0 0 9 0 0 0 66 0 0 0 MON 88017 2 0 0 0 9 0 0 0 66 0 0 0 MON 88017 × DAS-59122-7 2 0 0 0 9 0 0 0 66 0 0 0 MON 89034 2 0 0 0 9 0 0 0 66 0 0 0 MON 89034 × MON 88017 2 0 0 0 9 0 0 0 66 0 0 0 MON 89034 × MON 88017 × DAS-59122-7 2 0 0 0 9 0 0 0 26 0 0 0 MON 89034 × TC1507 2 0 0 0 9 0 0 0 66 0 0 0 MON 89034 × TC1507 × DAS-59122-7 2 0 0 0 9 0 0 0 56 0 0 0 MON 89034 × TC1507 × MON 88017 2 0 0 0 9 0 0 0 36 0 0 0 MON 89034 × TC1507 × MON 88017 × DAS-59122-7 2 0 0 0 9 0 0 0 66 0 0 0 TC1507 2 0 0 0 9 0 0 0 66 0 0 0 TC1507 × DAS-59122-7 2 0 0 0 9 0 0 0 66 0 0 0 Registered PIPs Total 26 0 0 0 117 0 0 0 778 0 0 0 
Monsanto Company CR240-13E3 Page 142 of 166 

Table 5 (continued). Proposed Acreage for 2013-2014 per State/Territory for P1–Inbred and Hybrid BreeNurseries 

Material 

AL 

AR 

CA 

CO 

GA 

HI 

IA 

ID 

IL 

IN 

KS 

Un-Registered PIPs 

MIR162 × MON87411 × DAS-59122-7 0 0 0 0 0 50 9 0 9 0 0 MON 87411 × DAS-59122-7 0 0 0 0 0 812 365 0 17 0 0 TC1507 × MON88017 DAS-59122-7 0 0 0 0 0 20 6 0 6 0 0 MIR162 × DAS-59122-7 0 0 0 0 0 20 6 0 6 0 0 MIR162 × MON 87411 0 0 0 0 0 86 16 0 16 0 0 TC1507 × MIR162 × MON87411 0 0 0 0 0 50 9 0 9 0 0 TC1507 × MIR162 0 0 0 0 0 110 9 0 9 0 0 MON 87411 0 0 2 0 0 249 29 0 29 0 0 MON 88017 × MON 87411 DAS-59122-7 0 0 0 0 0 100 9 0 9 0 0 MON 88017 × MON 87411 0 0 0 0 0 90 9 0 9 0 0 TC1507 × MON 88017 0 0 0 0 0 20 6 0 6 0 0 MON 89034 × DAS-59122-7 0 0 0 0 0 20 6 0 6 0 0 MON 89034 × MON 87411 × DAS-59122-7 0 0 0 0 0 20 6 0 6 0 0 MON 89034 × MIR162 0 0 2 0 0 551 210 0 21 0 0 MON 89034 × MIR162 × DAS-59122-7 0 0 0 0 0 20 6 0 6 0 0 MON 89034 × MIR162 × MON 87411 × DAS-59122-7 0 0 0 0 0 20 6 0 6 0 0 MON 89034 × MIR162 × MON 87411 0 0 0 0 0 87 17 0 17 0 0 MON 89034 × MIR162 × MON 88017 0 0 0 0 0 46 19 0 19 0 0 MON 89034 × MIR162 × MON 88017 × DAS-59122-7 0 0 0 0 0 26 13 0 13 0 0 MON 89034 × MON 87411 0 0 2 0 0 26 13 0 553 0 0 MON 89034 × MON 88017 × MON 87411 0 0 0 0 0 2 10 0 10 0 0 MON 89034 × TC1507 × MON 87411 × DAS-59122-7 0 0 0 0 0 88 18 0 18 0 0 
Monsanto Company CR240-13E3 Page 143 of 166 

Table 5 (continued). Proposed Acreage for 2013-2014 per State/Territory for P1–Inbred and Hybrid BreeNurseries 

Material 

MO 

MS 

NC 

ND 

NE 

OH 

OK 

PA 

PR 

SC 

SD 

TN 

Un-Registered PIPs 

MIR162 × MON87411 × DAS-59122-7 2 0 0 0 9 0 0 0 30 0 0 0 MON 87411 × DAS-59122-7 2 0 0 0 9 0 0 0 67 0 0 0 TC1507 × MON88017 DAS-59122-7 2 0 0 0 9 0 0 0 20 0 0 0 MIR162 × DAS-59122-7 2 0 0 0 9 0 0 0 20 0 0 0 MIR162 × MON 87411 2 0 0 0 9 0 0 0 66 0 0 0 TC1507 × MIR162 × MON87411 2 0 0 0 9 0 0 0 30 0 0 0 TC1507 × MIR162 2 0 0 0 9 0 0 0 30 0 0 0 MON 87411 2 0 0 0 9 0 0 0 69 0 0 0 MON 88017 × MON 87411 DAS-59122-7 2 0 0 0 9 0 0 0 60 0 0 0 MON 88017 × MON 87411 2 0 0 0 9 0 0 0 60 0 0 0 TC1507 × MON 88017 2 0 0 0 9 0 0 0 20 0 0 0 MON 89034 × DAS-59122-7 2 0 0 0 9 0 0 0 20 0 0 0 MON 89034 × MON 87411 × DAS-59122-7 2 0 0 0 9 0 0 0 20 0 0 0 MON 89034 × MIR162 2 0 0 0 9 0 0 0 66 0 0 0 MON 89034 × MIR162 × DAS-59122-7 2 0 0 0 9 0 0 0 20 0 0 0 MON 89034 × MIR162 × MON 87411 × DAS-59122-7 2 0 0 0 9 0 0 0 20 0 0 0 MON 89034 × MIR162 × MON 87411 2 0 0 0 9 0 0 0 67 0 0 0 MON 89034 × MIR162 × MON 88017 4 0 0 0 18 0 0 0 46 0 0 0 MON 89034 × MIR162 × MON 88017 × DAS-59122-7 2 0 0 0 9 0 0 0 26 0 0 0 MON 89034 × MON 87411 2 0 0 0 9 0 0 0 26 0 0 0 MON 89034 × MON 88017 × MON 87411 4 0 0 0 4 0 0 0 4 0 0 0 MON 89034 × TC1507 × MON 87411 × DAS-59122-7 2 0 0 0 9 0 0 0 68 0 0 0 
Monsanto Company CR240-13E3 Page 144 of 166 

Table 5 (continued). Proposed Acreage for 2013-2014 per State/Territory for P1–Inbred and Hybrid BreeNurseries 

Material 

AL 

AR 

CA 

CO 

GA 

HI 

IA 

ID 

IL 

IN 

KS 

KY 

Un-Registered PIPs 

MON 89034 × TC1507 × MIR162 0 0 0 0 0 432 206 0 17 0 0 MON 89034 × TC1507 × MIR162 × DAS-59122-7 0 0 0 0 0 20 6 0 6 0 0 MON 89034 × TC1507 × MIR162 × MON 87411 × DAS-59122-7 0 0 0 0 0 88 18 0 18 0 0 MON 89034 × TC1507 × MIR162 × MON 87411 0 0 0 0 0 87 18 0 17 0 0 MON 89034 × TC1507 × MIR162 × MON 88017 × DAS-59122-7 0 0 0 0 0 28 15 0 15 0 0 MON 89034 × TC1507 × MON 87411 0 0 0 0 0 27 14 0 14 0 0 MON 89034 × TC1507 × MON 88017 × MON 87411 × DAS-59122-7 0 0 0 0 0 86 16 0 16 0 0 TC1507 × MON 87411 × DAS-59122-7 0 0 0 0 0 27 14 0 14 0 0 TC1507 × MIR162 × DAS-59122-7 0 0 0 0 0 20 6 0 6 0 0 TC1507 × MIR162 × DAS-59122-7 × MON87411 0 0 0 0 0 20 6 0 6 0 0 TC1507 × MON 87411 0 0 0 0 0 27 14 0 14 0 0 Un-Registered PIPs Total 0 0 6 0 0 3375 1127 0 940 0 0 

Non-PIP 0 0 2 0 0 1047 118 0 118 0 0 

Border 0 0 0 0 0 1011 170 0 167 0 0 Total Acres 0 0 14 0 0 7311 1659 0 1469 0 0 
Monsanto Company CR240-13E3 Page 145 of 166 

Table 5 (continued). Proposed Acreage for 2013-2014 per State/Territory for P1–Inbred and Hybrid BreeNurseries 

Material 

MO 

MS 

NC 

ND 

NE 

OH 

OK 

PA 

PR 

SC 

SD 

TN 

Un-Registered PIPs 

MON 89034 × TC1507 × MIR162 2 0 0 0 9 0 0 0 67 0 0 0 MON 89034 × TC1507 × MIR162 × DAS-59122-7 2 0 0 0 9 0 0 0 20 0 0 0 MON 89034 × TC1507 × MIR162 × MON 87411 × DAS-59122-7 2 0 0 0 9 0 0 0 68 0 0 0 MON 89034 × TC1507 × MIR162 × MON 87411 2 0 0 0 9 0 0 0 68 0 0 0 MON 89034 × TC1507 × MIR162 × MON 88017 × DAS-59122-7 2 0 0 0 9 0 0 0 28 0 0 0 MON 89034 × TC1507 × MON 87411 2 0 0 0 9 0 0 0 27 0 0 0 MON 89034 × TC1507 × MON 88017 × MON 87411 × DAS-59122-7 2 0 0 0 9 0 0 0 66 0 0 0 TC1507 × MON 87411 × DAS-59122-7 2 0 0 0 9 0 0 0 27 0 0 0 TC1507 × MIR162 × DAS-59122-7 2 0 0 0 9 0 0 0 20 0 0 0 TC1507 × MIR162 × DAS-59122-7 × MON87411 2 0 0 0 9 0 0 0 20 0 0 0 TC1507 × MON 87411 2 0 0 0 9 0 0 0 27 0 0 0 Un-Registered PIPs Total 70 0 0 0 301 0 0 0 1293 0 0 0 

Non-PIP 100 0 0 0 110 0 0 0 647 0 0 0 

Border 100 0 0 0 165 0 0 0 514 0 0 0 Total Acres 296 0 0 0 693 0 0 0 3232 0 0 0 
Monsanto Company CR240-13E3 Page 146 of 166 

Table 6. Proposed Acreage for 2013-2014 per State/Territory for P2–Product Characteriz

Registered PIPs 

Material 

AL 

AR 

CA 

CO 

FL 

GA 

HI 

IA 

ID 

IL 

IN 

KS 

DAS-59122-7 10 11 2 25 6 6 12 54 0 54 35 44 MIR162 14 11 2 17 6 11 17 160 0 161 42 51 MON 88017 10 10 0 24 6 6 12 52 0 52 34 42 MON 88017 × DAS-59122-7 10 10 0 24 6 6 12 89 0 89 39 43 MON 89034 14 11 2 17 6 11 17 177 0 178 57 62 MON 89034 × MON 88017 10 10 0 24 6 6 12 74 0 74 34 38 MON 89034 × MON 88017 × DAS-59122-7 10 10 0 24 6 6 6 58 0 58 28 36 MON 89034 × TC1507 14 10 0 16 6 10 12 82 0 82 31 39 MON 89034 × TC1507 × DAS-59122-7 10 10 0 24 6 6 6 58 0 58 28 36 MON 89034 × TC1507 × MON 88017 10 10 0 24 6 6 12 44 0 44 34 38 

MON 89034 × TC1507 × 

MON 88017 × DAS-59122-7 10 10 0 24 6 6 12 132 0 132 49 57 

TC1507 10 11 2 13 6 7 12 41 0 42 23 32 TC1507 × DAS-59122-7 10 10 0 24 6 6 12 74 0 74 34 38 Total Registered PIP Acres 142 134 6 280 78 92 154 1093 0 1097 468 556 
Monsanto Company CR240-13E3 Page 147 of 166 

Table 6 (continued). Proposed Acreage for 2013-2014 per State/Territory for P2–Product Characterizatio

Registered PIPs 

Material 

MO 

MS 

NC 

ND 

NE 

OH 

OK 

PA 

PR 

SC 

SD 

TN 

TX 

DAS-59122-7 13 20 7 4 46 11 1 1 18 6 36 18 4 MIR162 18 28 7 4 76 26 2 1 23 6 39 22 10 MON 88017 12 20 6 4 44 10 0 0 18 6 36 18 2 MON 88017 × DAS-59122-7 12 20 6 4 55 15 0 0 18 6 41 18 2 MON 89034 18 28 7 4 82 41 2 1 23 6 54 22 10 MON 89034 × MON 88017 12 20 6 4 50 10 0 0 18 6 36 18 4 MON 89034 × MON 88017 × DAS-59122-7 6 14 6 4 42 4 0 0 12 6 34 12 4 MON 89034 × TC1507 12 22 6 4 44 15 0 0 18 6 29 22 8 MON 89034 × TC1507 × DAS-59122-7 6 14 6 4 42 4 0 0 12 6 34 12 4 MON 89034 × TC1507 × MON 88017 12 18 6 4 40 10 0 0 18 6 36 18 4 

MON 89034 × TC1507 × 

MON 88017 × DAS-59122-7 12 20 6 4 69 25 0 0 18 6 51 18 4 

TC1507 13 19 7 4 32 11 2 1 18 6 24 18 6 TC1507 × DAS-59122-7 12 20 6 4 50 10 0 0 18 6 36 18 4 Total Registered PIP Acres 158 262 80 52 670 190 5 4 232 78 486 234 66 
Monsanto Company CR240-13E3 Page 148 of 166 

Table 6 (continued). Proposed Acreage for 2013-2014 per State/Territory for P2–Product Characterizatio

Un-Registered PIPs 

Material 

AL 

AR 

CA 

CO 

FL 

GA 

HI 

IA 

ID 

IL 

IN 

KS 

KY 

MIR162 × MON87411 × DAS-59122-7 4 4 0 0 0 0 12 18 0 18 10 14 0 MON 87411 × DAS-59122-7 10 10 0 24 6 6 12 112 0 112 39 47 6 TC1507 × MON88017 DAS-59122-7 4 4 0 0 0 0 6 24 0 24 4 8 0 MIR162 × DAS-59122-7 4 4 0 0 0 0 6 24 0 24 4 8 0 MIR162 × MON 87411 10 10 0 24 6 6 12 44 0 44 34 38 6 TC1507 × MIR162 × MON87411 4 4 0 0 0 0 12 18 0 18 10 14 0 TC1507 × MIR162 4 4 0 0 0 0 12 28 0 28 10 14 0 MON 87411 10 11 2 25 6 7 12 155 0 156 50 59 6 MON 88017 × MON 87411 DAS-59122-7 4 4 0 0 0 0 12 73 0 73 15 19 0 MON 88017 × MON 87411 4 4 0 0 0 0 12 36 0 36 10 18 0 TC1507 × MON 88017 4 4 0 0 0 0 6 24 0 24 4 8 0 MON 89034 × DAS-59122-7 4 4 0 0 0 0 6 24 0 24 4 8 0 MON 89034 × MON 87411 × DAS-59122-7 4 4 0 0 0 0 6 24 0 24 4 8 0 MON 89034 × MIR162 14 14 4 19 6 12 17 154 0 156 45 56 10 MON 89034 × MIR162 × DAS-59122-7 4 4 0 0 0 0 6 24 0 24 4 8 0 MON 89034 × MIR162 × MON 87411 × DAS-59122-7 4 4 0 0 0 0 6 24 0 24 4 8 0 MON 89034 × MIR162 × MON 87411 10 12 0 24 6 8 12 47 0 48 36 40 6 MON 89034 × MIR162 × MON 88017 14 14 0 24 6 6 12 74 0 74 32 40 6 MON 89034 × MIR162 × MON 88017 × DAS-59122-7 10 10 0 24 6 6 6 50 0 50 28 32 6 MON 89034 × MON 87411 10 10 0 24 6 6 6 58 0 58 28 36 6 
Monsanto Company CR240-13E3 Page 149 of 166 

Table 6 (continued). Proposed Acreage for 2013-2014 per State/Territory for P2–Product Characterizatio

Un-Registered PIPs 

Material 

MO 

MS 

NC 

ND 

NE 

OH 

OK 

PA 

PR 

SC 

SD 

TN 

TX 

MIR162 × MON87411 × DAS-59122-7 12 12 0 4 14 10 0 0 12 0 12 12 0 MON 87411 × DAS-59122-7 12 20 6 4 64 15 0 0 18 6 41 18 4 TC1507 × MON88017 DAS-59122-7 6 6 0 4 12 4 0 0 6 0 10 6 0 MIR162 × DAS-59122-7 6 6 0 4 12 4 0 0 6 0 10 6 0 MIR162 × MON 87411 12 20 6 4 40 10 0 0 18 6 36 18 4 TC1507 × MIR162 × MON87411 12 12 0 4 14 10 0 0 12 0 12 12 0 TC1507 × MIR162 12 12 0 4 14 10 0 0 12 0 12 12 0 MON 87411 13 21 7 4 81 26 2 1 18 6 51 18 4 MON 88017 × MON 87411 DAS-59122-7 12 12 0 4 34 15 0 0 12 0 17 12 0 MON 88017 × MON 87411 12 12 0 4 18 10 0 0 12 0 12 12 0 TC1507 × MON 88017 6 6 0 4 12 4 0 0 6 0 10 6 0 MON 89034 × DAS-59122-7 6 6 0 4 12 4 0 0 6 0 10 6 0 MON 89034 × MON 87411 × DAS-59122-7 6 6 0 4 12 4 0 0 6 0 10 6 0 MON 89034 × MIR162 21 29 8 4 74 27 5 4 23 6 39 22 14 MON 89034 × MIR162 × DAS-59122-7 6 6 0 4 12 4 0 0 6 0 10 6 0 MON 89034 × MIR162 × MON 87411 × DAS-59122-7 6 6 0 4 12 4 0 0 6 0 10 6 0 MON 89034 × MIR162 × MON 87411 14 22 6 4 40 10 3 2 18 6 36 18 6 MON 89034 × MIR162 × MON 88017 12 20 6 8 50 8 0 0 18 6 44 18 4 MON 89034 × MIR162 × MON 88017 × DAS-59122-7 6 14 6 4 38 4 0 0 12 6 34 12 4 MON 89034 × MON 87411 6 14 6 4 42 4 0 0 12 6 34 12 4 
Monsanto Company CR240-13E3 Page 150 of 166 

Table 6 (continued). Proposed Acreage for 2013-2014 per State/Territory for P2–Product Characterizatio

Un-Registered PIPs 

Material 

AL 

AR 

CA 

CO 

FL 

GA 

HI 

IA 

ID 

IL 

IN 

KS 

KY 

MON 89034 × MON 88017 × MON 87411 0 0 0 0 0 0 2 8 0 8 0 4 0 MON 89034 × TC1507 × MON 87411 × DAS-59122-7 10 12 4 27 12 11 17 108 2 109 38 47 6 MON 89034 × TC1507 × MIR162 14 14 4 19 10 12 12 104 0 106 35 46 10 MON 89034 × TC1507 × MIR162 × DAS-59122-7 4 4 0 0 0 0 6 32 0 32 4 12 0 MON 89034 × TC1507 × MIR162 × MON 87411 × DAS-59122-7 10 10 0 24 6 6 12 99 0 99 39 43 6 MON 89034 × TC1507 × MIR162 × MON 87411 10 12 0 24 6 8 12 55 0 56 36 44 6 MON 89034 × TC1507 × MIR162 × MON 88017 × DAS-59122-7 10 10 0 24 6 6 6 58 0 58 28 36 6 MON 89034 × TC1507 × MON 87411 10 10 0 24 6 6 6 58 0 58 28 36 6 MON 89034 × TC1507 × MON 88017 × MON 87411 × DAS-59122-7 10 10 0 24 6 6 12 99 0 99 39 43 6 TC1507 × MON 87411 × DAS-59122-7 10 10 0 24 6 6 6 50 0 50 28 32 6 TC1507 × MIR162 × DAS-59122-7 4 4 0 0 0 0 6 24 0 24 4 8 0 TC1507 × MIR162 × DAS-59122-7 × MON87411 4 4 0 0 0 0 6 24 0 24 4 8 0 TC1507 × MON 87411 10 10 0 24 6 6 6 58 0 58 28 36 6 Total Un-Registered PIP Acres 242 249 14 402 112 124 300 1812 2 1820 686 878 110 

 Border 36 38 8 46 10 22 211 686 1 690 164 330 16  Non-PIP 34 50 21 39 10 28 286 849 1 872 103 598 14  Total 454 471 49 767 210 265 951 4440 4 4478 1421 2362 230 
Monsanto Company CR240-13E3 Page 151 of 166 

Table 6 (continued). Proposed Acreage for 2013-2014 per State/Territory for P2–Product Characterizatio

Un-Registered PIPs 

Material 

MO 

MS 

NC 

ND 

NE 

OH 

OK 

PA 

PR 

SC 

SD 

TN 

TX 

MON 89034 × MON 88017 × MON 87411 2 2 0 0 4 0 0 0 2 0 2 2 0 MON 89034 × TC1507 × MON 87411 × DAS-59122-7 14 20 7 4 64 17 1 2 18 6 41 18 7 MON 89034 × TC1507 × MIR162 16 24 8 4 54 17 5 4 18 6 29 22 14 MON 89034 × TC1507 × MIR162 × DAS-59122-7 6 6 0 4 16 4 0 0 6 0 10 6 0 MON 89034 × TC1507 × MIR162 × MON 87411 × DAS-59122-7 12 20 6 4 55 15 0 0 18 6 41 18 4 MON 89034 × TC1507 × MIR162 × MON 87411 14 22 6 4 44 10 3 2 18 6 36 18 6 MON 89034 × TC1507 × MIR162 × MON 88017 × DAS-59122-7 6 14 6 4 42 4 0 0 12 6 34 12 4 MON 89034 × TC1507 × MON 87411 6 14 6 4 42 4 0 0 12 6 34 12 4 MON 89034 × TC1507 × MON 88017 × MON 87411 × DAS-59122-7 12 20 6 4 60 15 0 0 18 6 41 18 4 TC1507 × MON 87411 × DAS59122-7 6 14 6 4 38 4 0 0 12 6 34 12 4 TC1507 × MIR162 × DAS-59122-7 6 6 0 4 12 4 0 0 6 0 10 6 0 TC1507 × MIR162 × DAS-59122-7 × MON87411 6 6 0 4 12 4 0 0 6 0 10 6 0 TC1507 × MON 87411 6 14 6 4 42 4 0 0 12 6 34 12 4 Total Un-Registered PIP Acres 310 444 108 132 1092 289 19 15 397 102 806 400 95 Border 219 231 14 52 400 120 10 8 221 10 302 218 28 Non-PIP 306 310 19 26 623 67 18 20 296 10 335 300 41  Total 993 1246 221 262 2785 666 52 47 1146 200 1929 1152 230 
Monsanto Company CR240-13E3 Page 152 of 166 

Table 7. Amount of Seed (pounds) Expected to be Planted in States Over the Duration of th

AL 

AR 

CA 

CO 

FL 

GA 

HI 

IA 

ID 

IL 

IN 

KS 

Border 1,692 1,786 376 2,162 470 1,034 57,434 40,241 47 40,288 7,708 15,510 DAS-59122-7 470 494 71 1,175 282 282 12,126 3,727 0 3,751 1,622 2,045 MIR162 × MON87411 × DAS-59122-7 188 188 0 0 0 0 2,914 1,269 0 1,269 470 658 MON 87411 × DAS-59122-7 470 470 0 1,128 282 282 38,728 22,410 0 6,054 1,833 2,209 TC1507 × MON88017 × DAS-59122-7 188 188 0 0 0 0 1,222 1,410 0 1,410 188 376 MIR162 658 517 165 799 282 494 12,361 8,709 0 8,756 1,974 2,397 MIR162 × DAS-59122-7 188 188 0 0 0 0 1,222 1,410 0 1,410 188 376 MIR162 × MON 87411 470 470 0 1,128 282 282 4,606 2,811 0 2,811 1,598 1,786 TC1507 × MIR162 × MON87411 188 188 0 0 0 0 2,914 1,269 0 1,269 470 658 TC1507 × MIR162 188 188 0 0 0 0 5,734 1,739 0 1,739 470 658 MON 87411 470 517 165 1,175 282 306 12,267 8,615 0 8,662 2,350 2,773 MON 88017 470 470 0 1,128 282 282 5,546 3,187 0 3,187 1,598 1,974 MON 88017 × DAS-59122-7 470 470 0 1,128 282 282 6,956 4,926 0 4,926 1,833 2,021 MON 88017 × MON 87411 × DAS-59122-7 188 188 0 0 0 0 5,264 3,854 0 3,854 705 893 MON 88017 × MON 87411 188 188 0 0 0 0 4,794 2,115 0 2,115 470 846 TC 1507 × MON 88017 188 188 0 0 0 0 1,222 1,410 0 1,410 188 376 MON 89034 658 517 71 799 282 494 13,301 9,508 0 9,555 2,679 2,914 MON 89034 × DAS-59122-7 188 188 0 0 0 0 1,222 1,410 0 1,410 188 376 MON 89034 × MON 87411 × DAS-59122-7 188 188 0 0 0 0 1,222 1,410 0 1,410 188 376 MON 89034 × MIR162 658 658 282 893 282 564 26,696 17,099 0 8,310 2,115 2,632 MON 89034 × MIR162 × DAS-59122-7 188 188 0 0 0 0 1,222 1,410 0 1,410 188 376 MON 89034 × MIR162 × MON 87411 × DAS-59122-7 188 188 0 0 0 0 1,222 1,410 0 1,410 188 376 MON 89034 × MIR162 × MON 87411 470 564 0 1,128 282 376 4,653 2,999 0 3,046 1,692 1,880 MON 89034 × MIR162 × MON 88017 658 658 0 1,128 282 282 2,726 4,362 0 4,362 1,504 1,880 MON 89034 × MIR162 × MON 88017 × DAS-59122-7 470 470 0 1,128 282 282 1,504 2,952 0 2,952 1,316 1,504 MON 89034 × MON 87411 470 470 94 1,128 282 282 1,504 3,328 0 28,708 1,316 1,692 MON 89034 × MON 88017 470 470 188 1,128 282 282 4,606 4,221 0 4,221 1,598 1,786 
Monsanto Company CR240-13E3 Page 153 of 166 

Table 7 (continued). Amount of Seed (pounds) Expected to be Planted in States Over the Duration 

MO 

MS 

NC 

ND 

NE 

OH 

OK 

PA 

PR 

SC 

SD 

TN 

Border 14,993 10,857 658 2,444 26,555 5,640 470 376 34,545 470 14,194 10,246 DAS-59122-7 682 940 306 188 2,562 494 24 24 3,948 282 1,692 846 MIR162 × MON87411 × DAS-59122-7 658 564 0 188 1,081 470 0 0 1,974 0 564 564 MON 87411 × DAS-59122-7 658 940 282 188 3,431 705 0 0 3,995 282 1,927 846 TC1507 × MON88017 × DAS-59122-7 376 282 0 188 987 188 0 0 1,222 0 470 282 MIR162 940 1,293 306 188 3,972 1,199 71 47 4,183 282 1,833 1,034 MIR162 × DAS-59122-7 376 282 0 188 987 188 0 0 1,222 0 470 282 MIR162 × MON 87411 658 940 282 188 2,303 470 0 0 3,948 282 1,692 846 TC1507 × MIR162 × MON87411 658 564 0 188 1,081 470 0 0 1,974 0 564 564 TC1507 × MIR162 658 564 0 188 1,081 470 0 0 1,974 0 564 564 MON 87411 705 964 306 188 4,207 1,199 71 47 4,089 282 2,397 846 MON 88017 658 940 282 188 2,491 470 0 0 3,948 282 1,692 846 MON 88017 × DAS-59122-7 658 940 282 188 3,008 705 0 0 3,948 282 1,927 846 MON 88017 × MON 87411 × DAS-59122-7 658 564 0 188 2,021 705 0 0 3,384 0 799 564 MON 88017 × MON 87411 658 564 0 188 1,269 470 0 0 3,384 0 564 564 TC 1507 × MON 88017 376 282 0 188 987 188 0 0 1,222 0 470 282 MON 89034 940 1,293 306 188 4,254 1,904 71 47 4,183 282 2,538 1,034 MON 89034 × DAS-59122-7 376 282 0 188 987 188 0 0 1,222 0 470 282 MON 89034 × MON 87411 × DAS-59122-7 376 282 0 188 987 188 0 0 1,222 0 470 282 MON 89034 × MIR162 1,081 1,363 376 188 3,901 1,269 235 188 4,183 282 1,833 1,034 MON 89034 × MIR162 × DAS-59122-7 376 282 0 188 987 188 0 0 1,222 0 470 282 MON 89034 × MIR162 × MON 87411 × DAS-59122-7 376 282 0 188 987 188 0 0 1,222 0 470 282 MON 89034 × MIR162 × MON 87411 752 1,034 282 188 2,303 470 141 94 3,995 282 1,692 846 MON 89034 × MIR162 × MON 88017 752 940 282 376 3,196 376 0 0 3,008 282 2,068 846 MON 89034 × MIR162 × MON 88017 × DAS-59122-7 376 658 282 188 2,209 188 0 0 1,786 282 1,598 564 MON 89034 × MON 87411 376 658 282 188 2,397 188 0 0 1,786 282 1,598 564 MON 89034 × MON 88017 658 940 282 188 2,773 470 0 0 3,948 282 1,692 846 
Monsanto Company CR240-13E3 Page 154 of 166 

Table 7 (continued). Amount of Seed (pounds) Expected to be Planted in States Over the Duration 

AL 

AR 

CA 

CO 

FL 

GA 

HI 

IA 

MON 89034 × MON 88017 × DAS-59122-7 470 470 0 1,128 282 282 1,504 3,32MON 89034 × MON 88017 × MON 87411 0 0 0 0 0 0 188 846 MON 89034 × TC1507 658 470 0 752 282 470 11,656 4,83MON 89034 × TC1507 × DAS-59122-7 470 470 0 1,128 282 282 2,914 3,32MON 89034 × TC1507 × MON 87411 × DAS-59122-7 470 564 188 1,269 564 517 4,935 5,91MON 89034 × TC1507 × MIR162 658 658 188 893 470 564 20,868 14,56MON 89034 × TC1507 × MIR162 × DAS-59122-7 188 188 0 0 0 0 1,222 1,78MON 89034 × TC1507 × MIR162 × MON 87411 × DAS-59122-7 470 470 0 1,128 282 282 4,700 5,49MON 89034 × TC1507 × MIR162 × MON 87411 470 564 0 1,128 282 376 4,653 3,42MON 89034 × TC1507 × MIR162 × MON 88017 × DAS-59122-7 470 470 0 1,128 282 282 1,598 3,42MON 89034 × TC1507 × MON 87411 470 470 0 1,128 282 282 1,551 3,37MON 89034 × TC1507 × MON 88017 470 470 0 1,128 282 282 3,196 2,81MON 89034 × TC1507 × MON 88017 × DAS-59122-7 470 470 0 1,128 282 282 4,606 6,94MON 89034 × TC1507 × MON 88017 × MON 87411 × DAS-59122-7 470 470 0 1,128 282 282 4,606 5,39Non-PIP 1,598 2,350 1,081 1,833 470 1,316 62,651 45,44TC1507 470 517 71 611 282 306 12,126 3,11TC1507 × DAS-59122-7 470 470 0 1,128 282 282 4,606 4,22TC1507 × MON 87411 × DAS-59122-7 470 470 0 1,128 282 282 1,551 2,99TC1507 × MIR162 × DAS-59122-7 188 188 0 0 0 0 1,222 1,41TC1507 × MIR162 × MON87411 × DAS-59122-7 188 188 0 0 0 0 1,222 1,41TC1507 × MON 87411 470 470 0 1,128 282 282 1,551 3,37Total 21,338 22,114 2,938 36,049 9,870 12,455 388,314 286,6
Monsanto Company CR240-13E3 Page 155 of 166 

Table 7 (continued). Amount of Seed (pounds) Expected to be Planted in States Over the Duration 

KS 

KY 

LA 

MI 

MN 

MO 

MS 

NC 

MON 89034 × MON 88017 × DAS-59122-7 1,692 282 282 188 1,551 376 658 282 MON 89034 × MON 88017 × MON 87411 188 0 0 0 0 282 94 0 MON 89034 × TC1507 1,833 470 470 658 2,632 658 1,034 282 MON 89034 × TC1507 × DAS-59122-7 1,692 282 282 188 1,551 376 658 282 MON 89034 × TC1507 × MON 87411 × DAS-59122-7 2,209 282 282 470 3,243 752 940 329 MON 89034 × TC1507 × MIR162 2,162 470 282 752 2,914 846 1,128 376 MON 89034 × TC1507 × MIR162 × DAS-59122-7 564 0 0 188 423 376 282 0 MON 89034 × TC1507 × MIR162 × MON 87411 × DAS-59122-7 2,021 282 282 470 3,243 658 940 282 MON 89034 × TC1507 × MIR162 × MON 87411 2,068 282 282 564 1,880 752 1,034 282 MON 89034 × TC1507 × MIR162 × MON 88017 × DAS-59122-7 1,692 282 282 188 1,551 376 658 282 MON 89034 × TC1507 × MON 87411 1,692 282 282 188 1,551 376 658 282 MON 89034 × TC1507 × MON 88017 1,786 282 282 470 1,927 658 846 282 MON 89034 × TC1507 × MON 88017 × DAS-59122-7 2,679 282 282 470 3,948 658 940 282 MON 89034 × TC1507 × MON 88017 × MON 87411 × DAS-59122-7 2,021 282 282 470 3,243 658 940 282 Non-PIP 28,106 658 658 2,891 6,956 19,082 14,570 893 TC1507 1,504 282 282 494 1,739 705 870 306 TC1507 × DAS-59122-7 1,786 282 282 470 2,303 658 940 282 TC1507 × MON 87411 × DAS-59122-7 1,504 282 282 188 1,551 376 658 282 TC1507 × MIR162 × DAS-59122-7 376 0 0 188 423 376 282 0 TC1507 × MIR162 × MON87411 × DAS-59122-7 376 0 0 188 423 376 282 0 TC1507 × MON 87411 1,692 282 282 188 1,551 376 658 282 Total 110,991 10,810 10,622 25,451 109,209 60,560 58,562 10,364 
Monsanto Company CR240-13E3 Page 156 of 166 

Table 7 (continued). Amount of Seed (pounds) Expected to be Planted in States Over the Duration 

OK 

PA 

PR 

SC 

SD 

TN 

TX 

WA 

MON 89034 × MON 88017 × DAS-59122-7 0 0 1,786 282 1,598 564 188 18MON 89034 × MON 88017 × MON 87411 0 0 282 0 94 94 0 0 MON 89034 × TC1507 0 0 3,948 282 1,363 1,034 376 47MON 89034 × TC1507 × DAS-59122-7 0 0 3,196 282 1,598 564 188 18MON 89034 × TC1507 × MON 87411 × DAS-59122-7 47 94 4,042 282 1,927 846 329 564 MON 89034 × TC1507 × MIR162 235 188 3,995 282 1,363 1,034 658 61MON 89034 × TC1507 × MIR162 × DAS-59122-7 0 0 1,222 0 470 282 0 18MON 89034 × TC1507 × MIR162 × MON 87411 × DAS-59122-7 0 0 4,042 282 1,927 846 188 47MON 89034 × TC1507 × MIR162 × MON 87411 141 94 4,042 282 1,692 846 282 47MON 89034 × TC1507 × MIR162 × MON 88017 × DAS-59122-7 0 0 1,880 282 1,598 564 188 18MON 89034 × TC1507 × MON 87411 0 0 1,833 282 1,598 564 188 18MON 89034 × TC1507 × MON 88017 0 0 2,538 282 1,692 846 188 47MON 89034 × TC1507 × MON 88017 × DAS-59122-7 0 0 3,948 282 2,397 846 188 47MON 89034 × TC1507 × MON 88017 × MON 87411 × DAS-59122-7 0 0 3,948 282 1,927 846 188 47Non-PIP 846 940 44,321 470 15,745 14,100 1,927 2,77TC1507 71 47 3,948 282 1,128 846 282 51TC1507 × DAS-59122-7 0 0 3,948 282 1,692 846 188 47TC1507 × MON 87411 × DAS-59122-7 0 0 1,833 282 1,598 564 188 18TC1507 × MIR162 × DAS-59122-7 0 0 1,222 0 470 282 0 18TC1507 × MIR162 × MON87411 × DAS-59122-7 0 0 1,222 0 470 282 0 18TC1507 × MON 87411 0 0 1,833 282 1,598 564 188 18Total 2,421 2,186 205,766 9,400 90,663 54,144 10,787 23,7
Monsanto Company CR240-13E3 Page 157 of 166 

Table 8. Proposed Amount of PIP Active Ingredient (grams) Over the Duration of the EUP for E

Cry1F Cry1A.105 Cry2Ab2 Vip3Aa20 Dv49 dsRNA Cry3Bb1 Cry3

AL 29.37 32.49 15.33 130.71 5.37E-05 78.01 34AR 29.53 32.52 14.83 131.57 5.62E-05 78.83 354CA 1.50 3.25 0.95 11.61 3.45E-06 2.65 11CO 53.53 61.14 29.48 191.76 1.06E-04 157.28 63FL 15.79 17.51 8.30 55.03 2.83E-05 39.91 16GA 16.97 20.24 9.63 69.22 2.96E-05 40.32 16HI 335.54 388.08 173.08 1889.25 8.11E-04 772.16 467IA 280.99 351.12 153.71 1444.24 6.46E-04 754.31 397ID 0.32 0.30 0.09 0.00 7.25E-07 0.23 4IL 251.77 376.37 161.29 1124.35 7.17E-04 967.32 328IN 84.95 97.51 47.27 351.71 1.74E-04 247.52 102KS 106.58 120.20 57.65 447.16 2.15E-04 304.14 128KY 15.47 18.23 9.03 61.91 2.61E-05 39.20 15LA 14.84 17.63 8.85 58.47 2.61E-05 39.20 15MI 27.24 29.25 13.59 146.62 5.53E-05 70.28 312MN 129.64 152.55 72.93 571.85 2.71E-04 367.95 165
Monsanto Company CR240-13E3 Page 158 of 166 

Table 8 (continued). Proposed Amount of PIP Active Ingredient (grams) Over the Duration of the EU

Cry1F Cry1A.105 Cry2Ab2 Vip3Aa20 Dv49 dsRNA Cry3Bb1 C

MO 40.58 43.17 19.28 208.96 8.60E-05 112.91 MS 51.87 56.72 26.39 244.65 1.05E-04 142.02 NC 14.76 16.84 8.09 55.46 2.67E-05 39.38 ND 13.90 14.26 6.31 68.79 2.76E-05 38.81 NE 159.09 180.05 83.30 696.97 3.29E-04 462.34 2OH 32.29 37.04 17.99 158.66 6.91E-05 90.53 OK 1.66 2.76 0.83 15.05 3.08E-06 1.00 PA 1.42 2.24 0.67 11.18 2.54E-06 0.82 PR 198.32 206.87 94.89 884.00 4.17E-04 550.32 2SC 14.21 16.02 7.85 51.60 2.61E-05 39.20 SD 96.00 108.53 51.59 399.00 1.97E-04 285.81 TN 47.69 49.93 23.85 216.70 9.43E-05 129.53 TX 12.79 16.02 7.35 60.19 2.00E-05 24.91 WA 26.21 25.62 11.99 126.41 5.48E-05 70.11 WI 78.40 85.85 41.15 310.00 1.57E-04 227.51 Total AI (grams) 2184.80 2580.30 1177.52 10193.08 4.84E-03 6175.68 2
Monsanto Company CR240-13E3 Page 159 of 166 

G.3 EUP Program Details 

G.3.a. Crop: Corn (Zea mays L.) 

G.3.b. Target Pests: Lepidopteran and Coleopteran insect pests 

G.3.c. Major Geographic Areas: EUP trials may occur in 30 states and Puerto Rico, 

representing diverse agronomic zones. Shipment (to/from) may occur in the United States and 

Puerto Rico. 

G.3.d. Sites: Research stations, seed production fields, privately owned farms, and 

university farms. 

G.3.e. Period of Use: Plantings from February 15, 2014 through February 28, 2016. 

G.3.f. Protocols for EUP Trials: 

Research trials planned under the EUP Program will be conducted under the following two 

protocols: 

1. Protocol 1 - Inbred and Hybrid Breeding and Observation Nurseries 

 Seed development and increase for future testing 

 Nursery observations of traits in various genetic backgrounds 

2. Protocol 2 - Product Characterization 

 Phenotypic and Agronomic Observations 

 Efficacy and Yield Benefits 

 Product Characterization, Performance, and Regulatory 
Monsanto Company CR240-13E3 Page 160 of 166 

G.3.f.1 Protocol 1 - Inbred and Hybrid Breeding and Observation Nurseries 

 Seed development and increase for future testing 

 Nursery observations of traits in various genetic backgrounds 

Objective 

 Introgress biotech corn events into elite inbred corn lines. 

 Produce research hybrid seed for evaluation in field, greenhouse, and/or laboratory trials. 

 Collect phenotypic data on converted inbred lines. 

 Collect plant tissue on converted inbred lines for laboratory analysis. 

 Seed is generally retained for future breeding or testing purposes. 

Description 

EUP Trials will generally be planted in a randomized block design or single large blocks. Plants 

may be hand- or open-pollinated with conventional or other biotechnology-derived corn. The 

corn may be self-pollinated, backcrossed, or otherwise crossed to other PIP or non-PIP plants. 

Seeds and/or plants may be treated with herbicides, plant growth regulators, fungicides and/or 

insecticides. Samples may be collected for analyses to determine phenotype, genotype, and/or 

other research purposes. 

Genotypes and Vectors 

The PIP test material will consist of insect-protected corn in different genetic backgrounds. 

Containment 

EUP Trials will be managed consistent with the containment procedures outlined in Section 

G.3.h., titled EUP Design Protocol. 
Monsanto Company CR240-13E3 Page 161 of 166 

G.3.f.2 Protocol 2 - Product Characterization 

 Phenotypic and Agronomic Observations 

 Efficacy and Yield Benefits 

 Product Characterization, Performance, and Regulatory 

Objective 

 Assess agronomic effects of the genetic insertion and combinations of biotech corn event 

in various genetic backgrounds under various environmental and insect pressure 

conditions. 

 Assess the yield and agronomic properties of EUP test inbreds and hybrids compared to 

other single and stacked event inbreds and hybrids (both registered and non-registered 

PIP single events and breeding stacks), commercial check hybrids (registered PIPs), 

conventional hybrids, and/or insecticide treatments. 

 Assess the herbicide tolerance of EUP test materials (including EUP test materials that 

have been crossed with non-PIP herbicide tolerant lines of corn). 

 Assess efficacy in various genetic backgrounds against lepidopteran, coleopteran, and 

other potential pests of corn. Develop data to assess the benefits of PIP test materials in 

various combinations. 

 Develop data to assess the benefits of MON 87411 and MIR162 alone and/or in 

combination with other test materials. 

 Produce grain and plant material for product characterization and performance studies. 

 Assess the level of control that PIP test plants provide of key lepidopteran, coleopteran, 

and other potential pests of corn. Develop data to assess the benefits of PIP test materials 

in various combinations. 

 Conduct field trials as needed to support global regulatory submissions. 

 Generate plant tissue samples from EUP test plants for use in regulatory and/or other 

research characterization studies. 

 Collect phenotypic data for use in global regulatory submissions. 

 To develop data to fulfill the requirements for Section 3 product registration under 

FIFRA. 

 Trials are generally terminal, whereas the seed may be disposed using approved methods 

outlined in Final Disposition. 

Description 

EUP Trials will generally be planted in a randomized block design or single large blocks. Seeds 

and/or plants may be treated with herbicides, plant growth regulators, fungicides and/or 

insecticides.

Phenotypic and Agronomic Observations

EUP Trials will be evaluated for several phenotypic and agronomic characteristics, such as 

seedling germination, seedling emergence, early vigor, plant color, plant height, days to 

flowering, days to maturity, yield and test weight. EUP trials may be assessed for herbicide 

tolerance and/or disease resistance resulting from genetic insertion and combinations of biotech 

corn events in various genetic backgrounds. 
Monsanto Company CR240-13E3 Page 162 of 166 

Efficacy and Yield Benefits 

EUP Trials may be evaluated for efficacy against lepidopteran, coleopteran and other corn pests 

and/or for yield. Artificial or natural infestations of lepidopteran and coleopteran may be used in 

these trials. Ears from EUP plants may be assessed for insect damage and mold incidence. Trials 

may include and/or be compared to non-transgenic or transgenic corn. Commercial checks may 

be planted as comparators. 

Product Characterization, Performance, and Regulatory

EUP trials may be evaluated for phenotypic/agronomic characteristics and plant tissue samples 

may be collected at various developmental growth stages for generating the basic 

characterization data including (but not limited to) composition, protein expression, and 

agronomic/phenotypic assessments. 

Data will be collected for the development of insect resistance management (IRM) strategies and 

to determine the level of control of lepidopteran, coleopteran, and other corn pests provided by 

the PIP test plants. Testing may include rating levels of damage and mortality for the relevant 

species in naturally and artificially infested trials. Trials may include non-transgenic or 

transgenic corn. 

Genotypes and Vectors 

The PIP test material will consist of insect-protected corn in different genetic backgrounds. 

Containment 

EUP Trials will be managed consistent with the containment procedures outlined in Section 

G.3.h., titled EUP Design Protocol. 
Monsanto Company CR240-13E3 Page 163 of 166 

G.3.g. EUP Design Protocol 

Seed generated under this EUP will not be offered for commercial sale or offered for use as food 

or feed. 

The following containment procedures will be used for all EUP Trials. 

Schedule 

 Hawaii and Puerto Rico plantings are continuous, but include a minimum 30-day 

irrigated fallow period after each harvest. In Puerto Rico, rainfall may substitute for 

irrigation. 

 The U.S. (excluding Hawaii) may have up to four plantings per site. Trials are generally 

planted February through July and harvested August through November. 

Storage of EUP Material 

 EUP test seed will be stored in a secure location and handled in a manner to prevent 

unintended release into the environment. 

 To prevent accidental mixing of EUP-regulated and non-EUP material, a uniform 

identification scheme should be implemented. Examples of identification include (but 

are not limited to) color coding, marking, tagging, or otherwise distinguishing seed to 

allow easy recognition. 

Shipment (Movement) of Regulated Plant Material 

 Packing Regulated Material – EUP test seed will be transported in secured, closed 

containers of such construction to avoid loss of seed outside the container during 

transport. The EPA stamped master label and a Monsanto EUP shipment summary label 

(see example) will be included with shipments of viable plant material (corn seed, whole 

plants, seedlings, immature embryos, pollen and cobs). Non-viable plant material (leaf 

tissue and roots) may be shipped without a label. 

 Labeling the Shipping Container – When shipping, the shipping container must be 

labeled with the crop, the EUP number, address, and number of containers. 

 
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Example: Monsanto EUP Shipment Summary Label 

Monsanto EUP Shipment Summary Label 

524-EUP-104 

Event Net contents (lbs) 

MON 87411 1.0 

MIR162 5.0 

MON 89034 x MIR162 6.5 

Non-PIP 2.5 

Border 10 

Planting 

 Trials will be planted at locations specified in USDA notifications or permits (if 

applicable), in states approved by the EPA Experimental Use Permit (EUP), and in 

locations approved according to EUP Section G.1.c. (See “Counties Where Experimental 

Trials will be Conducted”). 

 EUP trials will generally be planted in a randomized block design or single large blocks. 

 After planting is completed and before the planter leaves the field, the planter will be 

thoroughly cleaned out to prevent mixing with other corn seed. Seed will be disposed 

according to approved disposition methods (see “Final Disposition”). 

Isolation (Pollen Containment) 

 PIP test materials will be isolated to prevent persistence in the environment. Isolation 

methods will be implemented consistent with USDA Performance Standards at 7 CFR § 

340.3(c). 

Sampling 

 Plant tissue, seed, and/or whole plant samples may be collected during the growing 

season and shipped to Monsanto facilities and/or third party facilities for analysis. 

Harvest Procedures 

 Plots may be harvested by hand or mechanically. 

 EUP test material may be harvested for further research purposes. 

 Harvested ears, seed, or grain will be placed in labeled containers of such construction to 

prevent loss of plant material. 

 Equipment will be thoroughly cleaned after harvesting operations. 

 
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Final Disposition 

 Containers 

o May be inspected and cleared of remnant seed for reuse; or 

o Destroyed by burning, incineration, dry or steam heat, composting or buried in the 

test field, and/or shipped to approved outside processor for destruction 

 Leftover planting seed and harvested seed, grain, or plant material may be: 

o Used for other research purposes; or 

o Used as future planting seed; or 

o Returned to a secure on-site storage location; or 

o Returned to Monsanto facilities or third party facilities; or 

o Destroyed by dry or steam heat, burning or incineration, physical grinding, 

chemical application, composting or burying in the test field, or shipping to 

approved outside processor for destruction 

 Non-harvested plots (including border) and volunteer monitoring 

o Plant material and/or seed from non-harvested plots (including border) will be 

devitalized. Devitalization methods include (but are not limited to) hand 

weeding, grinding, burning or incineration, chemical application, or mechanical 

cultivation. 

o Volunteer plants will be routinely monitored and destroyed by hand weeding, 

grinding, burning or incineration, chemical application, or mechanical cultivation. 

Crop Rotation 

 Options for replanting the trial site the following growing season: 

o Leave the site fallow and control any volunteer plants. 

o Plant to another appropriate rotational crop that would allow the clear 

identification and control of volunteer corn plants. Replanting to corn not 

regulated by EPA or USDA is NOT allowed. 

o Plant the same or another regulated corn material and follow the requirements for 

that regulated corn material. 

o In Hawaii and Puerto Rico, continuous cropping of corn may occur, but must 

include at least a 30-day irrigated fallow period after each harvest, during which 

time the plots will be monitored for germinating corn plants. In Puerto Rico, 

rainfall may substitute for irrigation. 

Volunteer Monitoring 

 If the EUP trial is destroyed prior to flowering, no volunteer monitoring is required. 

 EUP plots that are allowed to flower will be monitored for volunteer plants to prevent 

persistence of EUP test plants in the environment. Any volunteer plants will be 

destroyed prior to flowering using approved Final Disposition methods (see “Nonharvested plots (including border) and volunteer monitoring”). 

 
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Termination of Experimental Use Program 

In the event that the EUP test plants receive EPA registration prior to the expiration date of the 

EUP, trials shall be managed as outlined below: 

 All terms and conditions of this EUP program shall be discontinued for trials that do not 

contain any unregistered PIPs, and registered PIPs shall be managed in accordance with 

the applicable registration. 

 Trials that contain unregistered PIPs shall be managed in accordance with the terms and 

conditions of the Experimental Use Permit.