Nanosys

Worker Activity EPA SanitizedTask Worker Activity Personal Protective Equipment/Eng

Controls Phy Forms and % product # Workers

Exposed Max

(hrs/day) Duration

(days/yr)

LS

‐ S1 Fume hood, closed vessel, Nitrile

gloves

, safety glasses, lab coat 0% 6 1.00 255

LS

‐ S2 Prepare Binned Core syringes based on calculation, fill x

number of 30 mL syringes to get to calculated amount

of 220

‐ 415 mL. Glove box (inert), safety glasses, lab

coat 0% 6 1.00 255

LS

‐ S3 Heat to 60°C. Evacuate the flask. Hold at 60 °C under

vacuum for 10

‐ 40 min. Start nitrogen purge. Closed vessel process in fume hood,

nitrile gloves, safety glasses, lab coat 0% 6 0.50 255

LS

‐ S4 Closed vessel process in fume hood,

nitrile gloves, safety glasses, lab coat 0% 6 2.00 255

LS

‐ S5 Glove box (inert), safety glasses, lab

coat 0% 6 2.00 255

LS

‐ S6 Closed vessel process in fume hood,

nitrile gloves, safety glasses, lab coat 6 1.00 255

LS

‐ S7 After addition and temperature ramp, hold at 210

‐ 220°C

for 10

‐ 12 min, cool to 50

‐ 70 C Closed vessel process in fume hood,

nitrile gloves

, safety glasses, lab coat 6 0.50 255

LS

‐ S8 Replace thermocouple and septa cap with secured glass

stoppers. Bring flask into the glove

‐ box under vacuum

using secondary containment, zip bag over the top and a

cart Fume hood, nitrile gloves, safety

glasses, lab coat and vinyl apron 6 0.50 255

LS

‐ S9 Move flask to glove box in a Ziploc bag and secondary

container. Pour 370

‐ 590 g of shell solution into 1 L

amber bottles and add 150

‐ 370 g of Toluene. Glove box (inert), glove box gloves

plus nitrile over gloves, safety glasses,

lab coat 6 1.00 255

LS

‐ S10 Quality Control

‐‐ Collect 14

‐ 16 mL in sealed cuvette.

Determine Quantum Yield, wavelength, and Full Width

Half Max (FWHM). Once analysis is complete liquid is

discarded into solvent waste. Glove box (inert), Nitrile gloves,

safety glasses, and lab coat. 2 0.3 255

LS

‐ S11 Add 66.5 mL of Growth Solution to a 250 mL centrifuge

tube bottle. Glove box (inert), glove box gloves

plus nitrile over gloves, safety glasses,

lab coat 3 4.00 255

LS

‐ S12 Glove box (inert), glove box gloves

plus nitrile over gloves, safety glasses,

lab coat 3 4.00 255

LS

‐ S13 Remove from glove

‐ box in bottles, shake vigorously for

30sec and centrifuge for 15min at 4000 RPM Nitrile gloves, safety glasses, and lab

coat. 3 4.00 255

LS

‐ S14 Bring back into glove

‐ box in bottles and pour off

supernatant. Dissolve the precipitates in 13 mL of

Toluene and combine into single container. Rinse bottles

with remaining toluene. Glove box (inert), glove box gloves

plus nitrile over gloves, safety glasses,

lab coat 3 4.00 255

LS

‐ S15 Glove box (inert), glove box gloves

plus nitrile over gloves, safety glasses,

lab coat 3 4.00 255

LS

‐ S16 Quality Control

‐‐ Dilute 20μL of this solution in 4 mL of

hexane. Filter and determine the optical density using

the UV/vis spectrometer. Material is placed into cuvette

in preparation for removal from glove box. Once

analysis is complete liquid is discarded into solvent

waste. Glove box (inert), Nitrile gloves,

safety glasses, and lab coat.

3 1.00 255

LS

‐ S17 Laboratory glassware is placed into a 30% Potassium

Hydroxide (KOH) / 70% Isopropyl Alcohol (IPA) base

bath for a minimum of thirty minutes. Splash protection including gloves

(PVC/Butyl/Neoprene outer gloves in

combination with inner nitrile gloves),

safety glasses, splash shield, lab coat

and splash apron. 0.01% of residual

quantum dots 3 0.50 255

LS

‐ S18 Retrieve glassware and pour off any residual base bath

and gently submerge into sodium bicarbonate rinse bath

to neutralize the 30% Potassium Hydroxide (KOH) / 70%

Isopropyl Alcohol (IPA). Splash protection including gloves

(PVC/Butyl/Neoprene outer gloves in

combination with inner nitrile gloves),

safety glasses, splash shield, lab coat

and splash apron. 0% 3 0.10 255 Lab Scale Shell Reaction Procedure

Lab Scale Shell Wash Procedure

Lab Scale Glassware Washing

PMN Page 9 Attachment

Section A--(2) Occupational Exposure

Page 1 of 3Nanosys

Worker Activity EPA SanitizedTask Worker Activity Personal Protective Equipment/Eng

Controls Phy Forms and % product # Workers

Exposed Max

(hrs/day) Duration

(days/yr)

LS

‐ S19 Retrieve glassware from neutralizer bath and place into

nitric acid bath bin. Splash protection including gloves

(PVC/Butyl/Neoprene outer gloves in

combination with inner nitrile gloves),

safety glasses, splash shield, lab coat

and splash apron. 0% 3 0.10 255

LS

‐ S20 Place glassware in bin and transport to rinse sink.

Glassware is washed minimal of two times with tap

water, inspected and then rinsed with deionized water. Nitrile gloves, safety glasses, and lab

coat. 0% 3 0.10 255

LS

‐ S21 Glassware is placed on pegs to dry. If the wet glasswear

is needed hastily, it can be rinsed with ethanol and then

drip dried before placing in a designated glass drying

oven (100° C). Glassware is dried for a minimum of

fifteen minutes before being removed. Thermal gloves, nitrile gloves, safety

glasses, and lab coat.

0% 3 0.20 255

LS

‐ S22 The hot glass should be cooled in a designated area with

visible warning signs for hot glassware. Once cooled and

safe to handle with clean nitrile gloves, the glassware

can be restocked in appropriately labeled locations and

is ready for use. Nitrile gloves, safety glasses, lab coat.

0% 3 0.20 255

GEN II

S1 Closed tank system using flexible SS

hose to transfer from transfer tank to

Shell Reactor tank: nitrile gloves,

safety glasses, lab coat; face shield

used during all hose connections and

disconnections. 0% 1 0.25 255

GEN II

S2 Heat to 60°C. Evacuate the Shell Reactor. Hold at 60 °C

under vacuum for 30

‐ 40 min. This is a closed system and personnel

where lab coat, gloves, safety glasses,

and face shield with connecting and

discounting lines 0% 1 0.25 255

GEN II

S3 Closed tank system using flexible SS

hose to transfer from transfer tank to

Shell Reactor tank: nitrile gloves,

safety glasses, lab coat; face shield

used during all hose connections and

disconnections. 0% 1 0.25 255

GEN II

S4 Closed tank system using flexible SS

hose to transfer from transfer tank to

Shell Reactor tank: nitrile gloves,

safety glasses, lab coat; face shield

used during all hose connections and

disconnections. 0% 1 0.25 255

GEN II

S5 Closed tank system using flexible SS

hose to transfer from transfer tank to

Shell Reactor tank: nitrile gloves,

safety glasses, lab coat; face shield

used during all hose connections and

disconnections. 0% 1 0.25 255

GEN II

S6 Closed tank system using flexible SS

hose to transfer from transfer tank to

Shell Reactor tank: nitrile gloves,

safety glasses, lab coat; face shield

used during all hose connections and

disconnections. 1 0.25 255

GEN II

S7 Closed tank system using flexible SS

hose to transfer from transfer tank to

TFF Feed Tank: nitrile gloves, safety

glasses, lab coat; face shield used

during all hose connections and

disconnections. 1 0.25 255 GEN II Shell Reaction Procedure

GEN II Shell Tangential Flow Filtration (TFF) Process

PMN Page 9 Attachment

Section A--(2) Occupational Exposure

Page 2 of 3Nanosys

Worker Activity EPA SanitizedTask Worker Activity Personal Protective Equipment/Eng

Controls Phy Forms and % product # Workers

Exposed Max

(hrs/day) Duration

(days/yr)

GEN II

S8 Closed tank system using flexible SS

hose to transfer from transfer tank to

TFF Feed Tank/TFF: nitrile gloves,

safety glasses, lab coat; face shield

used during all hose connections and

disconnections. 1 0.25 255

GEN II

S9 Quality Control

‐‐ Dilute 20μL of this solution in 4 mL of

hexane. Filter and determine the optical density using

the UV/vis spectrometer. Material is placed into cuvette

in preparation for removal from glove box. Once

analysis is complete liquid is discarded into solvent

waste. Glove box; Nitrile gloves, safety

glasses, and lab coat.

1 0.25 255

GEN II

S10 All tanks and lines are cleaned with a clean in place

system using Toluene at 50 C. This is a closed system and personnel

where lab coat, gloves, safety glasses,

and face shield with connecting and

discounting lines 0.01% of residual

quantum dots 1 0.25 255

GEN II

S11 All Transfer tanks and flexible lines are taking to the

stationary pumped cleaning system using Toluene. This is a closed system and personnel

where lab coat, gloves, safety glasses,

and face shield with connecting and

discounting lines 0.01% of residual

quantum dots 1 0.25 255

End of Document GEN II Clean In Place System

PMN Page 9 Attachment

Section A--(2) Occupational Exposure

Page 3 of 3