REVISED DATA EVALUATION RECORD

VEGETATIVE VIGOR EC 25 TEST

GUIDELINE 123

- 1B (TIER II)

1. CHEMICAL : Sulfentrazone (129081)

2. TEST MATERIAL : Sulfentrazone technical; 94.2%

3. CITATION :

Author: Chetram, R.S.

Date: 1992

Title: Tier 2 vegetative vigor nontarget phytotoxicity study using F6285 technical

Laboratory: Pan

- Agricultural Laboratories, Inc., Madera, CA

Report No. : BL91

- 474

Sponsor: FMC Corporation, Princeton, NJ

MRID No.: 433454

- 12 (report)

437836

- 02 (raw data)

Note: The r aw data file includes original test concentrations in addition to continuation studies for species

in which a NOAEC was not achieved. The continuation studies had treatment concentrations that were

lower than the original s tudy and in many cases the endpoints were derived from this data.

4. ORIGINALLY REVIEWED BY :

William Erickson

Biologist

EEB/EFED/EPA

1992

5. Re

- reviewed By:

Geoffrey Sinclair Signature:

Biologist

EPA/EFED/ERB5 Date: 1/29/15

6. STUDY PARAMETERS/RESULTS SYNOPSIS :

Definitive study duration: 21 days

Species tested: oat, ryegrass, corn, onion, soybean, le ttuce, tomato, radish, cabbage and

cucumber

Most sensitive species: cucumber

Measurement endpoint: dry weight

A new EC25 for cucumber dry weight w as calculated after reanalysis of the raw dat a with EFED’s CETIS

software. Results are summarized below.

E C

25 : 0 .001617 lb a. i

. /acre

95% C onfidence Interval (CI): 0.000679

- 0.002693 lb ai/acre

NOEC: 0.00044 lb ai/acre3

` The previous data analysis (from 1992) resulted in the following EC 25 and CI

. EC 25 : 0.00052 lb ai/acre

95% CI: 0.00024

- 0.0011 lb ai/acre

NOEC: 0.00044 lb ai/acre

These values were used in the 2014 preliminary risk assessment for sulfentrazone.

Although the EC25 changed, the NOEC remained the same, 0.00044 lbs ai/acre.

7. CONCLUSION : The study is scientifically sound and fulfills the guideline requirement for a Tier 2

plant vegetative vigor study. The CETIS statistical output is attached to this DER.

8. ADEQUACY OF THE STUDY : Core.

9. MAJOR GUIDELINE DEVIATIONS : None.

10. MATERIALS AND METHODS :

Test Organisms:

Guideline Criteria Reported Information

Species: (6 dicots in 4 families including

soybean and rootcrop; 4 monocots in 2 families

including corn) Monocots: corn, ryegrass,

oat, onion

Dicots: soybean, tomato,

cucumber, radish,

lettuce, cabbage

No. plants per rep.: (5)

No. reps per dose: (3) 5 plants per rep;

4 reps per dose

Source of seed reported? Y es

Test System:

Guideline Criteria Reported Information

Solvent: A cetone

Test site: G reenhouse

Soil type: pasteurized sandy loam (0.3% organic matter) with perlite

added to facilitate drainage

Fertilizer: none reported

Pot size and description: plastic pots

(7.5 x 7.5 x 6.0 cm)

No. plants per pot: 54

` Guideline Criteria Reported Information

Method of pesticide

application: spray booth equipped with a single TeeJet 8001

- E nozzle and a

compressed air cylinder

Watering method and

frequency: (under foliage) w atered by hand as needed during first 48 h after treatment;

thereafter,

watered using a HydroRain controlled irrigation system

Test duration: 21 days

Growth stage at

application: (past first true

leaf stage) 1

- 3 true leaf stage

Test Design:

Guideline Criteria Reported Information

Dose range: (2X or 3X) 3X

Doses: (at least 5) 6

Controls: (negative and solvent ) solvent control

Parameters observed and measured shoot weight (dry),

shoot height,

survival,

phytotoxic symptoms

Maximum labeled application rate: 0.375 lb ai/acre

(from label)

11. REPORTED RESULTS :

Guideline Criteria Reported Information

NOEC Observed? Y es

Measured Initial Chemical

Concentrations? (optional) Y es

Raw data included? (Y/N) Y es

(tomato data in MRID#

433454

- 12; others in

MRID# 437836

- 02)

Quality Assurance Measures? Y es5

` Shoot weight (dry), shoot height, plant survival: The mea surement endpoint with the most sensitive

EC 25 value is tabulated below for each of the 10 species. The most sensitive monocot and dicot

species are highlighted in bold.

Statistical Methods: EC 25

- regression analysis

NOEC

- Dunnett's Test

Phytotoxic symptoms: Ten days after treatment, all crops exhibited significant symptoms, mainly foliar

desiccation and burning. The NOECs (lb ai/acre) for the species tested, in order of increasing sensitivity

are as follows:

corn (0.167) < lettuce = radish = oat = ryegrass (0.019) < cabbage = onion (0.006) < tomato =

soybean (0.00133) < cucumber (0.00044) Species Endpoint EC 25

(lb ai/A) NOEC

(lb ai/A)

Monocots

corn dry weight 0.24 0.167

ryegrass dry weight; plant height 0.051 0.019

oat dry weight 0.023 0.019

onion dry weight 0.0067 0.006

Dicots

soybean dry weight 0.0044 0.00133

lettuce dry weight 0.020 0.006

cabbage dry weight 0.0099 0.006

radish dry weight 0.044 0.019

cucumber dry weight 0.0015 0.00133

tomato dry weight 0.0024 0.001336

` Mortality: Plant Mortality (no. dead plants out of 20 total) by day 21 is tabulated below.

Species Dose (lb ai/acre)

0 0.002 0.006 0.019 0.056 0.167 0.50

corn 0 0 0 0 0 0 12

ryegrass 0 0 0 0 0 11 20

oat 0 0 0 0 0 0 12

onion 1 2 4 7 12 20 20

soybean 0 0 0 0 0 0 5

lettuce 0 0 0 0 4 17 20

radish 0 0 0 0 0 1 6

cabbage 0 0 0 0 2 10 19

cucumber 0 0 0 6 20 20 20

T omato 0 0 0 0 19 20 20

12

. REVIEWER'S ANALYSIS :

Previous Analysis done in 1992

Statistical Methods: EPA/OPP/EFED Nuthatch Program (results attached)

EC 25

- weighted nonlinear regression

NOEC

- Williams' Test

Endpoint: dry weight

EC 25 : 0.00052 lb ai/acre

95% Confidence Limit ( C.L. ) : 0.00024

- 0.0011 lb ai/acre

NOEC: 0.0004 lb ai/acre

Slope: 2.28

Most sensitive monocot: onion

Endpoint: dry weight

EC 25 : 0.012 lb ai/acre

95% C.L.: 0.0056

- 0.025 lb ai/acre

NOEC : 0.006 lb ai/acre

Slope: 2.447

` Revised Analysis with CETIS (2015)

Most sensitive dicot: cucumber

End point: dry weight

E C 25 = 0.001617 lb ai/acre

95% CI: 0.000679

- 0.002693 lb ai/acre

NOEC = 0.00044 lb ai/acre

These analyses were conducted using CETIS version 1.8.7.12 with backend settings approved for use by

EFED on 5/29/13.

Species Endpoint EC 25

(lb ai/A) NOEC

(lb ai/A)

Monocots

C orn dry weight 0.18 0.056

ryegrass dry weight 0.045 0.019

O at dry weight 0.033 0.006

O nion dry weight 0.012 0.006

Dicots

soybean dry weight 0.0044 0.0013

L ettuce dry weight 0.025 0.006

C abbage dry weight 0.011 0.006

R adish dry weight 0.057 0.019

c ucumber d ry weight 0.001617

( 0.00052 * ) 0.00044

T omato dry weight 0.012 0.0013

* Value from original 1992 analysis.

12. CONCLUSIONS : The study is scientifically sound and fulfills the guideline requirement for a Tier 2

vegetative vigor study. Cucumber was the most sensitive species for both dry weight (EC 25 = 0.00 1617 lb

ai/acre) and plant height (EC 25 = 0.0032 lb ai/acre) measurement endpoints, but soybean (dry weight EC 25 = 0.0044 lb ai/acre) also was highly sensitive.