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# CHEMICAL HAZARD DATA AVAILABILITY STUD Y
What Do We Really Know About the Safety of High

Production Volume Chemicals ?

EPA's 1998 Baseline of Hazard Information that is Readily Available to the Publi c Prepared by EPA's Office of Pollution Prevention and Toxics (April 1998)

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CHEMICAL HAZARD DATA AVAILABILITY STUDY What do we Really Know About the Safety of High Production Volume Chemicals? The 1998 Baseline of Hazard Information that is Readily Available to the Publb Q This repo rt presents the results of EPA's analysis of test data availability for 2,863 organic chemicals produced or imported at or above I million pounds per year in the United States . The repo rt includes a chemical-by-chemical presentation of the hazard information available to the public on each of the US High Production Volume (HPV) chemicals . The US produces or imports close to 3,000 chemicals (excluding polymers and inorganic chemicals) at over 1 million pounds per year . A fundamental question concerns whether, and to what extent, basic toxicity information is available to the public on these major commercial chemicals in the United States . One might assume that basic toxicity information is available for these chemicals so that producers, users, workers, and consumers could be aware and be able to evaluate the hazards and risks posed by the chemicals they encounter in their daily lives . Based on results from a recent analysis done by EPA, this is not a prudent assumption . EPA's analysis found that no basic toxicity information, i .e., neither human health nor environmental toxicity, is publicly available for 43% of the high volume chemicals manufactured in the US and that a full set of basic toxicity information is available for only 7% of these chemicals. The lack of availability of basic toxicity information on most high volume chemicals is a serious issue for several reasons . For EPA, the availability of hazard information on individual chemicals is fundamental to many of the Agency's approaches to accomplishing its mission of environmental protection - risk assessment, safeguarding children's health, expanding the public's right-to-know, and promoting the pollution prevention ethic . Longrange strategic planning at EPA has recently been formalized and expanded as a result of the Agency-wide effort to implement the Government Performance and Results Act (GPRA) of 1993 . Strategic program objectives under the Toxic Substances Control Act (TSCA) for EPA's Office of Pesticides, Prevention, and Toxic Substances, incorporated into the Agency-wide strategic plan, call for EPA to significantly increase the introduction by industry of safer or "greener" chemicals which will decrease the need for regulatory management by EPA, and to improve the ability of the public to reduce exposure to specific environmental and human health risks by making current, accurate substance-specific information widely and easily accessible . Steps to ensure the availability of basic toxicity information on HPV chemicals would be an integral part of meeting these objectives . Congress acknowledged the need for data in identifying, controlling and preventing the possible hazardous effects of chemicals on health and the environment when it passed TSCA (P .L . 94-469, October 11, 1976) . TSCA states that it is the policy of the US that manufacturers are responsible for testing chemicals, and the Act gives EPA some authority to require chemical testing and impose controls as necessary . In practice, implementation has been difficult for a variety of reasons and the rate of testing has been slow . It is time to fill the gap in information about these high volume chemicals . US companies need to do much more testing and generate basic toxicity assessments in a form that is publicly available and usable by consumers, community groups, chemical users, and workers . Industry has committed to increase testing, but their progress has been slow; meanwhile companies continue to reap significant profits from the production and sale of these HPV chemicals . What Tests are Necessary? International authorities agree that testing in six basic endpoint areas (known as "basic tests" in this report) is necessary for a minimum understanding of a chemical's toxicity . These tests cover : acute toxicity; chronic toxicity; developmental and reproductive toxicity ; mutagenicity; ecotoxicity ; and environmental fate . This basic level of testing and other information is called the Screening Information Data Set, or SIDS . The SIDS includes information on the identity of the chemical, its physical and chemical properties, uses, sources and extent of 2

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exposure . The testing required is designed to answer basic questions about the chemical . For instance, environmental fate testing can indicate whether the chemical degrades quickly in the environment and how it is distributed throughout the environment . Acute toxicity testing is designed to measure how toxic the chemical is from acute or one-time exposures, such as from accidental ingestion or skin contact . Other tests measure the effects from longer exposures as might be encountered in the workplace or in communities near production facilities, such as subchronic toxicity testing and mutagenicity tests (which could indicate a potential to cause cancer) . Tests are also required that measure the chemical's ability to interfere with reproduction (fertility) and fetal development . Finally, a number of studies are required to indicate the potential for environmental effects such as to fish, invertebrates, and aquatic plants should the chemical be released to water from production and wastewater treatment facilities . While these tests do not fully measure a chemical's toxicity, they do provide a minimum set of information that can be used to determine the relative hazards of chemicals and to judge if additional testing is necessary . Background While there is much work to be done, it is true that some progress has been made in improving our understanding of chemical hazards and risks in the past twenty years . The National Academy of Sciences National Research Council (NAS/NRC, 1984) studied this question in the mid-1980s and concluded that "minimal" toxicity information was available for only 22% of high volume chemicals .' The Organization for Economic Cooperation and Development (OECD), recognizing this problem in the late 1980s, initiated a voluntary program to assure that basic information is available on international high production volume (HPV) chemicals (OECD, 1990) . This program, known as the Screening Information Data Set or SIDS program, has produced an internationally-agreed-upon set of screening tests2 and is working to complete the SIDS data set for international HPV chemicals (OECD, 1998a) . To date, the OECD has initiated or completed work on over 300 HPV chemicals (OECD, 1998b) . The US has committed to handling 25% of the HPV chemicals as its contribution to the OECD SIDS effort ; other countries have committed to handling chemicals in proportion to the size of their gross domestic product . Because most of the international HPV chemicals are also commercially available in the US, EPA considers the OECD/SIDS program to be an integral part of domestic testing activities . EPA has committed to partnering with industry in the US and abroad to achieve the common goal of accomplishing this minimum level of testing for HPV chemicals and will work with other parties (international organizations, environmental groups, unions, other Federal agencies, environmental justice groups and others) to secure their interest and support for this effort . Nevertheless, because of the slow pace of the international efforts to generate the needed data, it will be necessary for the US to accelerate its efforts in order to ensure the availability of the data needed to support US domestic efforts on chemicals . Last year, the Environmental Defense Fund (EDF, 1997) reported in "Toxic Ignorance" the results of its analysis of the availability of basic health test data on HPV chemicals and concluded that only 29% of the HPV chemicals in the US met the minimum data requirements for health hazard screening established by the OECD/SIDS program . Note that the percentages reported in EDF's conclusions were based on a selected sample of 100 HPV chemicals, and deal only with human health hazard information, which is a subset of the full SIDS described above . EDF subsequently challenged the US chemical industry to confront this issue and

1The National Research Council defined "minimal toxicity information" for industrial chemicals as the availability of an y one or more of the five test types included in their study : acute toxicity ; subchronic toxicity ; chronic toxicity ; reproductiveldevelopmental toxicity ; mutagenicity. 2 The OECD Screening Information Data Set consists of the following required tests : Physical/Chemical Properties - melting/boiling point ; water solubility ; vapor pressure ; octanoUwater partition coefficient ; water solubility. Environmental Fate - aerobic biodegradation . Environmental Toxicity - acute toxicity to fish, Daphnia, and algae . Toxicological Data - acute toxicity; repeated dose toxicity ; genetic toxicity ; reproductive/developmental toxicity. 

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accept the responsibility for assuring completion of and public access to health screening test data on HPV chemicals . The Chemical Manufacturers Association ( CMA) conducted its own analysis of the sample of 100 HPV chemicals and concluded that 47% of the chemicals had full SIDS health data sets - a conclusion which, although quantitatively different from EDF's, is consistent with the view th at significant basic testing requirements remain to be filled for the HPV chemicals . With regard to indust ry response to the EDF challenge, CMA, its member companies, and others in the US chemical indust ry have expressed an interest and willingness to engage both EDF and EPA in a constructive dialogue to address the significant issues raised in the "Toxic Ignorance" repo rt . CMA, on behalf of its member companies, previously agreed to the policy adopted by the OECD that high production volume status alone is sufficient to establish the need for a full SIDS data set . Unfortunately, there is a major inconsistency between CMA's Responsible Care/Product Stewardship Program and the organization's w ritten commitment to the OECD/SIDS program in that the Responsible Care/Product Stewardship codes do not include a commitment to ensure that SIDS data sets are developed and made publicly available for HPV chemicals . Recent developments indicate that CMA is conside ri ng a number of initiatives to increase indust ry participation in SIDS testing, including increasing its volunta ry commitment to the SIDS program . CMA has also initiated an expansive search effo rt to determine the actual amounts and types of SIDS data which are available for HPV chemicals . These are encouraging signs, but must be followed by concrete actions . EPA concluded that a comprehensive analysis looking at the full set of US HPV chemicals was needed to determine the public availability of basic screening data on these chemicals . This baseline report is the result of that analysis, and should clarify the magnitude of the problem and establish a basis against which future improvements can be judged . EPA intends to update this search pe ri odically to measure progress against these initial baseline results . EPA recognized the importance of and welcomed EDF's evaluation and its challenge to the chemical indust ry on this critical issue . While the EDF report's challenge to US indust ry focuses on human health-related infomiation, EPA believes that the full SIDS data set (which includes environmental fate and effects information) is needed . EPA applauds the willingness of both EDF and CMA to join in this "constructive discussion" and th e Agency welcomes st rong participation from other environmental groups and a broader range of stakeholders . It is encouraging that these major organizations are in agreement with the basic message that more testing is needed . Method The evaluation of the availability of hazard data for US HPV chemicals included a search for basic health and environmental information on these chemicals, as well as a compa ri son of available information for various subsets of the 3,000 HPV chemicals . Important aspects to the current methodology include : 1 . The search for available hazard data was restricted to information in the Chemical Information System ( CIS, 1998). 2 . Assessment of va rious subsets of chemicals (such as Toxic Release Invento ry chemicals, or chemicals with an associated Occupational Health and Safety Administration Permissible Exposure Limit) are based only on the subset of those chemicals identified as HPV . 3. The analysis focused on identifying "publicly available" data on these HPV chemicals . EPA considered "publicly available" data as meeting the following requirements : that full descripti ons of th e study method and the results obtained were accessible to the public . The data can exist in the form of a journal a rticle or as a publicly accessible study repo rt such as a study submitted to EPA under sec tion 4 or sections 8(d)/8(e) of TSCA. EPA recognized that 4

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while various publicly accessible databases point to the existence of test data (in the form of brief data summaries), the studies cited in these data bases do not meet the requirement that "full descriptions of the study method and the results obtained" were publicly accessible and thus have not been included in this analysis .3 Creation of the US HPV Hazard Databas e A database containing the results of a search for available hazard data on US HPV chemicals (excluding polymers and inorganics) was created for use in this analysis . These chemicals are restricted to those for which data were submitted to EPA under the 1990 TSCA Inventory Update Rule . TSCA chemicals do not include cosmetics, drugs, food and food additives, pesticides, nuclear material, firearms, tobacco and tobacco products . A brief description of the format of this database is provided in Appendix I . The database will soon be available on EPA's Chemical Testing and Information High Production Volume website at www.epa .gov/opptintr/chemtest/hpv .htm . An extensive search for all publicly available hazard data was not conducted due to limited resources ; thus, the search was restricted to information in the Chemical Information System (CIS) . It is noted that the search strategy used in this study is not comprehensive and will have missed studies that were not included in the data bases searched . Studies contained in databases which did not meet the criteria for Opublicly available" data, such that full descriptions of the study method and results obtained were available to the public, have also been excluded from the analysis . The CIS was originally formed under joint contract to EPA and NIH, and is a publicly accessible commercial vendor for nearly 100 databases . Among its varied topics, the CIS includes a number of different source collections and datasets which document available data related to the SIDS test battery which served as the basis for evaluating chemicals in these analyses . As noted, the six endpoints comprising the SIDS test battery are acute toxicity, chronic toxicity, developmental and reproductive toxicity, mutagenicity, ecotoxicity, and environmental fate . Details of the CIS search are presented below . The Chemical Abstract Services (CAS) registry numbers for the approximately 3,000 HPV chemicals were used to search the CIS databases . CAS numbers, as opposed to chemical names, were used as a matching criteria because CAS numbers are uniquely assigned to chemicals, while chemicals are often known by multiple names . In addition to its multiple individual data sources which can be searched, the CIS contains a separate system, known as the Structure and Nomenclature Search System (SANSS), that can be used to identify which of the individual data sources contain information for a particular chemical . Although the SANSS is an easier way to identify available information across all of the individual data sources, it is not updated as frequently as the individual data sources . For this reason, some of the individual data sources (RTECS, TSCATS, IRIS, GENETOX, AQUIRE, Phytotox, and Envirofate) were searched independently of the search that was performed using SANSS . The eleven CIS-SANSS data sources which were used in the search for available hazard data are listed below . Descriptions of each of the data sources are presented in Appendix II .

Registry of Toxic Effects of Chemical Substances (RTECS) Toxic Substances Control Act Test Submissions (TSCATS) Integrated Risk Information System (IRIS ) Chemical Carcinogenesis Research Information System (CCRIS) GENETO X 3One example can be found in a paper prepared by the Chemical Manufacturers Association (CMA, 1997) which included EUCLID, a data base prepared by the European Union . In many instances, the information which is available on studies included in EUCLID is limited to the summary provided in the database, thus additional documentation as to the study method and details on the results is not publicly available for many of the studies . 

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Environmental Mutagen Information Center (EMIC ) Environmental Teratology Information Center (ETIC) Catalog of Teratogenic Agents (CTA) Aquatic Information Retrieval Database (AQUIRE) Phytotox Envirofate For each SIDS endpoint, Table A lists the individual data sources which were searched, along with the specific keywords used, where applicable . 

# Table A. Individual Data Sources Searched for Available Hazard Data on Each

SIDS Test Componen t SIDS Test Component Data Source Search Keyword Acute Toxicity RTECS TO X TSCATS HE-ATOX Chronic Toxicity TSCATS HE-CTOX IRIS HAZ Rf D Developmental & Reproductive RTECS TE R Toxicity TSCATS H E-RTOX-TE RA ETIC CTA Mutagenicity RTECS MU T TSCATS HE-GTOX GENETOX EMIC Ecotoxicity AQUIR E TSCATS EE-ATOX, EE-CTOX, EE-RTOX Phytotox Environmental Fate TSCATS EF Envirofate This analysis focused on a "best case" scena rio in that credit for data availability was given to several endpoints even though the complete SIDS testing requirement for that endpoint may not be met . Specifi cally, for rep roductive/developmental toxicity, mutagenicity, ecotoxicity, and environmental fate tests, the analysis accepted th e availability of at least one test in each endpoint area as being sufficient to suggest that th e SIDS need was filled ( in fact, multiple tests are required to fulfill the SIDS test requirements for these endpoints) . In addition, no assessment of data quality was made for the data located in the various searches . The mere existence of data meeting the publicly available requirements warranted an "X" in the column for an endpoint . Thus, th e analysis only points to th e availability of information . W hat Basic Screening and Testing Has Been Done : Ove rview of Result s Tables/Figures 1 and 2 presented in Appendix III provide highlights from the analysis of US HPV chemicals to determine th e availabili ty of key test data . Examination of these tables and figures leads to the following obse rvations : 43% of the US HPV chemicals (1,216 of 2,863) are identified as having pQ SIDS test data ( Le., no basic screening data) of any type available and only 7% of the chemicals (

202 of 2,863) are reported as having data available for all of the SIDS data types . These statistics indicate that there are many US HPV chemicals for which numerous SIDS data gaps exist and that there is a continuing need for significantly expanded testing efforts by US 6

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chemical companies to deal with this important problem . Some 277 of the 2,863 U .S . HPV chemicals are part of the ongoing OECD SIDS international testing program, in which companies in various countries around the world have voluntarily agreed to conduct the SIDS battery of tests on selected chemicals . Most of the OECD SIDS test data are not yet publicly available, but they will become accessible as they are incorporated into existing databases . Some 46 of the 277 U .S . HPV chemicals have already completed the SIDS test battery under the OECD program . For 9 of those chemicals, all of the test data are publicly available (and are included in the counts listed in Table 2) ; for the remaining 37 completed chemicals, one or more of the tests, while complete, are not yet available through the publicly accessible databases searched in this study. Adding these 37 chemicals to the number of chemicals for which all SIDS data are available (202 chemicals) increases the percentage of fully-tested chemicals from 7 .1 % to 8 .3% . Com parisons to Other Studie s The National Academy of Sciences National Research Council (NAS/NRC, 1984) studied theavailability of "minimal toxicity information" for existing chemicals in the TSCA inventory in the mid1980s . NRC defined "minimal toxicity information" for industrial chemicals as the availability of any one or more of the five test types included in their study . The first column of Table 3 shows the availability of hazard data information for 259 US HPV chemicals based on the analysis conducted by NAS/NRC in 1984 . This information was extracted from Auer and Gould (1987) . NRC concluded that one of five toxicity tests (acute toxicity, subchronic toxicity, chronic toxicity, reproductive/developmental toxicity, and mutagenicity) was available for only 22% of the randomly selected set of 259 HPV chemicals . In contrast, EPA's current analysis located at least one of the human health hazard data components for 55% of the 2,863 HPV chemicals . A more direct comparison can be made by comparing the percent of chemicals with test data for a specific hazard endpoint . For instance, NAS/NRC located information on acute toxicity for only 20% of the 259 HPV chemicals . In contrast, EPA's current study was able to find test data on acute toxicity for almost 50 percent of the 2,863 HPV chemicals . In 1997, the Environmental Defense Fund (EDF) reported in "Toxic Ignorance" (EDF, 1997) the results of its analysis of the availability of basic human health test data on a sample of 100 HPV chemicals . The Chemical Manufacturers Association (CMA, 1997) conducted its own analysis of these same 100 HPV chemicals to determine whether there are additional publicly available data which were not reflected in the EDF report . Their results of these analyses on the EDF 100 HPV chemicals and EPA's analysis results on the 2863 HPV chemicals are summarized in Table 3 . EDF's analysis estimated that only 29% of the 100 HPV chemicals met the minimum data requirements for health hazard screening established by the OECD/SIDS program . On the other hand, CMA concluded that 47% of the chemicals had full SIDS health data available . EPA's analysis estimated that only 9% of all 2863 US HPV chemicals met the data availability requirements for human health hazard screening . EPA's investigation of the extent to which hazard data is available for the nearly 3000 US HPV chemicals is proceeding . However, the results to date indicate that significant and serious data gaps do exist related to the availability of hazard information for many of the chemicals most widely used in the United States . An examination of Table 3 indicates that while the conclusions of previous studies and EPA's current assessment are quantitatively different, they are consistent with the view that significant basic testing requirements remain to be filled for the HPV chemicals . Table 3 also indicates that this remains true even though the amount of hazard information that is publicly available for US HPV chemicals has substantially increased in the past decade . 

# HPV Chemicals and Right-to-Know - What Information Is Readily Available to Communities?
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Test Data Availability of Selected Chemicals in the Toxic Release Inventory TRI Database The Toxic Release Inventory (TRI), initiated by EPA in 1987, provides information to the public on releases and other waste management information for more than 600 chemicals and chemical categories from certain industry sectors . Facilities report their TRI information annually to EPA . The information includes : the amounts of each listed chemical released to the environment at the facility ; amounts of each chemical shipped offsite for recycling, energy recovery, treatment, or disposal ; amounts of each chemical recycled or burned for energy recovery, or treated at the facility ; and maximum amounts of the chemical present at the facility during the year . With this information, communities know what toxic chemicals are present in their neighborhoods, and facility managers can identify opportunities for materials management, source reduction, pollution prevention, and cost savings
. A search of the HPV chemicals included in the 1995 Toxic Release Inventory (TRI) was conducted to determine what SIDS testing information was publicly available for these chemicals . As shown in Table 4 and Figure 3, 203 HPV chemicals appear on the TRI list . As a result of its review, EPA has learned that many chemicals may have never been tested to determine how toxic they are to humans or the environment . (A number of these chemicals are also pesticides, but, this analysis did not consider information developed under the Federal Fungicide, Insecticide, and Rodenticide Act (FIFRA)) . While one might expect TRI chemicals to be relatively well tested, in fact, there are numerous testing gaps . The subset of 203 HPV chemicals in the 1995 TRI list yielded better results than for HPV chemicals as a whole - the full six-test SIDS battery was available for - 54% of the chemicals and all of the TRI HPV chemicals had at least some data available . Nonetheless, the TRI chemicals showed some significant gaps in the basic data set -- about 20% of the TRI HPV chemicals were missing 2 or more of the basic SIDS tests . On the other hand, however, is the observation that the majority of HPV chemicals not listed on TRI lack the basic information needed to determine whether they should be listed on TRI . For example, - 46% of the non-TRI HPV chemicals have n2 data available and less than 4% have the full set of basic tests. The HPV TRI chemicals with higher exposure potential were also examined and 91 (out of the 203) HPV TRI chemicals were identified with reported total (on-site and off-site) releases at levels greater than 1 million pounds (for the 1995 reporting year) . Of these 91 high release HPV TRI chemicals, 74% have information available on all six basic SIDS tests, an additional 20% had five of the SIDS tests available, and all of the high release HPV TRI chemicals have data available from at least three of the SIDS tests . See Table 5 and Figure 4 in Appendix III . Nonetheless, given the large releases and potential for exposure, it is clear that testing beyond the basic SIDS level is necessary to adequately understand the risks of such high exposure chemicals . HPV Chemicals and Worker Safety Test Data Availability of Chemicals wi th Occuoational Safety and Health Administration (OSHA) Permissible Exoosure Limits The Federal Occupational Safety and Health Administration (OSHA) sets Permissible Exposure Limits (PELs) for hazardous chemicals in the workplace . These PELs are limits, usually calculated on an eight hour time weighted average basis, on inhalation exposures developed to protect worker health . As another means of evaluating the US HPV chemicals, EPA obtained an electronic file of the 738 chemicals currently subject to occupational exposure controls set by OSHA . Based on information in this file, EPA identified the US HPV chemicals which have OSHA PELs . 

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Up to six separate measures of permissible exposure limits were defined in the file . These measures were : control limit PEL in mg/m3, control limit PEL in ppm, time weighted average PEL in mg/m3, time weighted average PEL in ppm, short term exposure limit PEL in mg/m3, or short term exposure limit PEL in ppm . For the purposes of this evaluation, a chemical was considered to have an OSHA PEL if any of the six limits were defined . Among the 738 chemicals in the file, 288 chemicals were excluded from the analysis because they did not have defined OSHA PELs . Of the 450 chemicals with OSHA PELs, 17 chemicals did not have CAS numbers and were also excluded from the analysis . After crossreferencing the remaining 433 OSHA PEL chemicals with the US HPV list, 193 of them were found to be high production volume chemicals . EPA assessed the extent to which hazard data are publicly available for these 193 HPV OSHA PEL chemicals as compared with the remainder of the US HPV chemicals . The results of human health hazard data availability for OSHA PEL chemicals are summarized in Table 6 and Figure 5 . EPA found that only 52% of these HPV chemicals with PELs have basic screening tests for all four of the human health endpoints (acute toxicity, chronic toxicity, developmental/reproductive toxicity, and mutagenicity) . In contrast, of the HPV chemicals without PALS, only 5% had all four health data types and 49% had p2 data of any type available ; more than 80% have hazard data available on two or fewer SIDS health data components . Thus, it is clear that the bulk of HPV chemicals without PELs lack even the minimal test data needed to support development of a PEL value to protect workers . HPV Chemicals and Widely-Used Consumer Products Test Data Availability of Chemicals Believed to be Contained in Consumer Products Of particular interest are the statistics showing the degree to which untested chemicals are used in everyday consumer products found in the average American home . Another major concern is the range of uses and the volumes of HPV chemicals present in the materials used in construction of the homes, schools, churches, offices, factories, shopping malls, and other buildings in which Americans spend most of their time . The point here is not to identify specific brands or manufacturers ; the point is that many generic product types found in every home contain chemicals that are untested for toxicity . EPA is developing the source ranking database (S .D .) which among other things identifies the sources of consumer product chemicals (CPC) . There are 1418 chemicals in the S .D ., of which 1303 have unique CAS numbers . Among these 1303 chemicals, 491 are US HPV chemicals . EPA assessed the extent to which hazard data are publicly available for the 491 US HPV-CPC chemicals found in the S. D . . The results of hazard data availability for these consumer product chemicals are summarized in Table 7 and Figure 6 . As the figures indicate, relative to other subsets of chemicals, substances used in consumer products appear to have received a greater level of screening testing than is typical for all HPV chemicals . However, chemicals used in consumer products warrant a higher level of testing than that provided by the SIDS set in order to adequately assess potential risks . As with the high release TRI chemicals, there is high exposure potential to chemicals in consumer products, including exposure to children . Significantly greater amounts of testing are needed to demonstrate the safety of such consumer chemicals given the large and varied makeup of the exposed population .



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Do Chemical Manufacturers Ensure that Test Data are Made Publicly Available on the Chemicals They Make ? Analyses were also conducted to determine the proportion of hazard data available for US high producti on volume (HPV) chemicals for each manufacturer/impo rter of these chemicals . The analysis focused on the six basic tests previously identified to comp rise a minimally acceptable screening information data set (SIDS) and, for each HPV chemical, the fraction of the six components of hazard information for which data were available was computed . For example, a given chemical might have hazard data available for only acute toxicity and ecotoxicity. This chemical has available information for two of th e six hazard data components and th e proportion of hazard data available for this chemical is 2/6 . For each company, the average proportion of hazard data available for all of the HPV chemicals manufactured by that company was computed . For example, suppose that a chemical company manufactures four chemicals and that the number of hazard data components available for the four chemicals are 1, 2, 0, and 3 . The average propo rt ion of the six hazard data components available for the four chemicals manufactured by that company is (1/6 + 2/6 + 0/6 + 3/6)/4 = 1/4 . The average proporti on is 1 if all six hazard components are available for each HPV chemical produced/imported by the company and is 0 if none of the components are available for any of the chemicals produced/impo rt ed by the company . The results of the analysis are summa rized in Figure 7 . Average proporti ons of hazard data available ranged fro m 0 to 1 (6/6) . For graphical purposes, the average propo rtions were grouped into seven catego ries : 0/6 ; > 0/6 - < 1 .5/6 ; 1 .5/6 -- < 2 .5/6 ; 2 .5/6 - - < 3 .5/6 ; 3 .5/6 -< 4 .5/6 ; 4 .5/6 -< 6/6, and 6/6 . The first catego ry includes only those companies that manufacture/impo rt chemicals that do not have any hazard data publicly available . The second through sixth categories include companies for which the average proportion of hazard data available falls within th e ranges indicated . These catego ri es include companies that manufacture just one HPV chemical whose data availability proportion fell into one of th e ranges to companies that manufacture hundreds of chemicals where the average p ro portion of hazard data available for chemicals manufactured /i mported by the company fell into a given range . The seventh catego ry includes those companies that manufacture/impo rt chemicals for which all hazard data are publicly available . Th e analysis covered 830 manufacturers/impo rters of US HPV chemicals . As illustrated in Figure 7, all of the chemicals manufactured/imported by 148 of the 830 (18%) companies have no SIDS data available ( i .e., 0/6 SIDS tests are available) . This category is compri sed of fewer than 250 chemicals . An additional 459 companies (55%) sell products for which, on average, half (3/6) or less of the SIDS tests are publicly available . Finally, for only 21 of the 830 (3%) companies can it be said that all six of the SIDS tests (6/6) are publicly available for all of their chemicals ( fewer than 40 chemicals comprise this catego ry) . The Bottom Line -- How Much Will it Cost to Get the Remaining Basic Information? The Cost of the SIDS ScreeningTests And the Profitability of HPVC s Any additional testing or screening of chemicals has a price tag . For each chemical, the basic set of six SIDS screening test endpoints costs about $205,000
. Assuming th at (1) test data identified in this study as publicly available meet minimum criteria as to quali ty and usability and (2) where multiple studies are needed to complete an endpoint ( i .e., mutagenicity, reproductive/ developmental toxicity, ecotoxicity, and environmental fate), all such studies were in fact available, Table 8 shows the esti mated testing costs to complete remaining SIDS testing for all HPV chemicals . (Test data which may currently exist in restricted access files but is subsequently made publicly available would serve to lower th ese costs .) It would cost the chemical Industry approximately 0.2% of the total annual 10

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sales of the top 100 US chemical companies to rill all of the basic screening set data gaps for the high production volume chemicals . This represents less than $427 million of the 1996 industry sales figure of $231 billion (Chemical and Engineering News, May 1997) . EPA believes, however, that for many of these chemicals, the SIDS battery of tests will not provide sufficient understanding to adequately assess the hazards and risks presented by some chemicals . Table 8 presents the specific higher tier tests that may be necessary to adequately assess the hazards of higher exposure chemicals (e .g ., chemicals in consumer products, chemicals to which children may be exposed, high release TRI chemicals, chemicals with large numbers of exposed workers, etc .) and the costs for those tests . Where high exposure was not a major concern, the results of the SIDS battery of tests (identified as "Level 1(SIDS)" tier) would be used to determine which of the assays outlined in the SIDS+ and SIDS++ batteries are a priority such that higher level testing is necessary . Next step s EPA plans to use the results of this analysis to establish a baseline of available testing and measure progress in generating missing needed test data against that baseline . This report will also be of value to improving company product stewardship efforts, such as CMA's Responsible Care program, because of the fundamental issues which it poses concerning implementation of effective product stewardship efforts in the absence of even basic information on chemical hazards and risks . With this baseline report as a point of departure, EPA is gathering additional information to begin to engage manufacturers, interest groups, other Federal agencies, and academia in an effort to set priorities for completing the SIDS tests for the remaining HPV chemicals and to set goals for an aggressive testing program that will produce the hazard information on these chemicals which is needed by the public, industry, and others . For example, EPA could establish a challenge program for the industry to produce this testing by the end of 2003 or 2005 . That would make the cost to industry between $61-85 million per year - representing approximately 0 .04% of the chemical industry's annual sales . Considering that there are about 260 million American citizens, using the 2005 end date, that would amount to less than a quarter per year for each citizen over the seven years of testing .

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Reference s Auer, C .M ., Gould D .H . (1987) Carcinogenicity assessment and the role of structure activity relationship (SAR) analysis under TSCA Section 5 . Journal of Environmental Science and Health . C5(1) : 29-71 . Chemical Manufacturers Association . (1997) "Definition, Content and Availability of Databases Searched ." Appendix A to Draft Report (as yet unpublished), attached to CMA Letter to Environmental Defense Fund and EPA, September 19, 1997 . Environmental Defense Fund . (1997) "Toxic Ignorance ." Environmental Health Program of Environmental Defense Fund . Summer 199 7 National Research Council (NRC) . (1984) : Toxicity Testing : Strategies to Determine Needs and Priorities . National Academy Press, Washington, DC, 1984 . OECD . (1990) Decision-Recommendation on the Co-operative Investigation and Risk Reduction of Existing Chemicals - C(90)163/FINA L OECD . (1998a) Encyclopedia of Toxicology, Volume 2, pages 460-461 (Publ . Academic Press, 1998, Ed . Philip Wexler) OECD . (1998b) OCED press release on HPV chemicals is available on the Internet at http ://www.oecd .org/news_and_events/release/nw-38a .htm . UNEP . (1996) United Nations Environmental Program : "Screening Information Data Set (SIDS) for High Production Volume Chemicals", Volumes 1-4, Published by the United Nations, New York, NY and Geneva, Switzerland, March, 1996



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APPENDIX I . 

# HPV Hazard Database : Description of Database Format

14

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The U .S . High Production Volume (HPV) chemicals database contains information on whether or not eight hazard data components are publidy available for 2931 U .S . HPV chemicals . Six of the hazard data components (acute toxicity, chronic toxicity, developmental and reproductive toxicity, mutagenicity, ecotoxicity, and environmental fate) comprise the "Screening Information Data Set" (SIDS) test battery established by the Organization for Economic Cooperation and Development (OECD, 1998a) . Variable names for each column are shown in the first row . The remaining rows contain the information on hazard data availability for the chemicals . The first column (CAS) contains a chemical identity number : Chemical Abstract Services registry number . CAS numbers are uniquely assigned to chemicals . The name of the chemical is displayed in the second column (CHEMNAME) . An "X" is shown in the third column (ACUTE), if EPA was able to locate any information on acute toxicity testing . Columns 4 (CHRONIC), 5(DEVEL/REPRO), 6 (MUTAGEN), 7 (ECOTOX), and 8 (FATE) are also marked with an "X" if hazard data were located for chronic toxicity, neurotoxicity, developmental/reproductive toxicity, mutagenicity, carcinogenicity, ecotoxicity, and environmental fate, respectively . The total number of eight hazard data test components located for each chemical is shown in Column 9 (TOTAL) . Some 277 of the 2931 U .S . HPV Chemicals are part of the ongoing OECD SIDS international program . Some of the OECD SIDS test data have not been entered into the databases searched in this study . The database will be updated to include that information once the hazard data become available
. A "C" or "U" is marked in Column 10 (SIDS) if the chemical is part of SIDS testing program
. A "C" indicates that testing has been completed, and a "U" denotes that testing is ongoing . Copies of completed SIDS dossiers are available through the United Nations Environmental Programme (UNEP, 1996) . The table below displays the information contained in the database for three sample chemicals . The CAS number and chemical name of the first chemical are 71432 and benzene . As shown below, all six hazard tests have been conducted for benzene, and the chemical is active under the SIDS program . On the other hand, only three of the hazard tests (acute toxicity, ecotoxicity, and environmental fate) were located for hexene (CAS no . 25264931), and none of the six were located for 1-octanesulfonyl fluoride (CAS no . 40630635) . This database will be available at EPA's Chemical Testing and Information High Production Volume website at www.epa .gov/opptintr/chemtest/hpv.htm .

, ._.. E 71432 Benzene X X X X X X U 25264931 Hexene X X X ~~~ 1-Ocfanesulfonyl 15

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## p . 105

APPENDIX H . 

# Where can the Public Find the Information? : Selected Data Sources

16

## p . 106

Data Sources Available through the Chemical Information System (CIS) -- Call 1-800-247-8737 for information on CIS . 1 . Rggis~ of Toxic Effects of Chemical Substances T(RECS)o RTECS is a comprehensive database of basic toxicity information for over 100,000 chemical substances . It is maintained by NIOSH, and contains quarterly updates from 1971 to present . In addition to toxic effects and general toxicology reviews, data on skin and/or eye irritation, mutation, reproductive consequences, and tumorigenicity are provided . Toxic effects are linked to literature citations from both published and unpublished government reports (including unpublished test data from TSCATS, the EPA TSCA test submissions database), and published articles from the scientific literature . The database can also be searched online through the TOXNET system . URL : http ://sis .nim .nih .gov 2 . Toxic Substances Control Act Test Submissions (TSCATS) : TSCATS is a database that indexes unpublished health and safety studies, chemical test data, and substantial risk data submitted to EPA under TSCA sections 4, 8(d), 8(e), and For Your Information" ("FYI") . It was developed by Syracuse Research Corporation for EPA's Office of Toxic Substances, and is a means of conveying the Agency's receipt of unpublished, non-confidential studies covering test results and adverse effects of chemicals on health and ecological systems submitted under TSCA . TSCATS catalogs the purpose of testing (observations sought), the test organisms used, the routes of administration, and, where available, a description of the nature of the chemical tested (e .g ., pure, component of a mixture) . The title of the submission is given, as well as file identification data . The database can also be searched online through the NLM Medlars system . URL : http ://sis .nlm .nih .gov 3 . Integrated Risk Information System (IRIS) : IRIS, prepared and maintained by EPA, is an electronic database containing health risk and EPA regulatory information on specific chemicals . IRIS was developed by EPA staff in response to a growing demand for consistent risk information on chemical substances for use in decision-making and regulatory activities . IRIS is designed for EPA staff, but is also accessible to state and local environmental health agencies . The information in IRIS is intended for individuals with extensive training in toxicology, but with some knowledge of health sciences . The database can also be searched online through the TOXNET system . URL : http ://sis .nlm .nih .gov 4 . Chemical Carcinoaenesis Research Information System (CCRIS) : CCRIS contains over 7,300 chemical records and is sponsored by the National Cancer Institute . It contains scientifically evaluated data derived from carcinogenicity, mutagenicity, tumor promotion, and tumor inhibition studies . The database can also be searched online through the TOXNET system . URL : http ://sis .nim .nih .gov 5. GENETOX : GENETOX is a collection of genetic assay studies developed through the collaborative efforts of the National Institute of Environmental Health Sciences, the Oak Ridge National Laboratory, and the Environmental Mutagen Information Center (EMIC) . The database is directed toward the goal of establishing standard genetic testing and evaluation procedures for the purposes of regulating toxic substances and determining the direction of research and development in this area . Assays are compared on a chemical-by- chemical basis . Each GENETOX record corresponds to an individual chemical and may incorporate several studies . Assay results are included on mutagenicity tables . These tables provide specific information on the type of assay, the biological host, the assay endpoint, and the final qualitative results of the assay . When available, reference information for the assays is also given . GENETOX data are derived from the results of assays selected from published primary papers and from reports submitted to EMIC from contractors . Chemical data included in the database comes from the following sources : EMIC (Environmental Mutagen Information Center), ETIC (Environmental Teratogen Information Center), SOLM, WATER POLLUTION, OSHA (Occupational Safety and Health Administration), CHEMLINE, TSCA (Toxic Substances Control Act) Lists, and RTECS (Registry of Toxic Effects of Chemical Substances) . The database can also be searched online through the TOXNET system . URL : http ://sis .nim .nih .gov 6 . Aquatic Information Retrieval Database QUIRE) : AQUIRE is produced by the EPA Environmental Research Laboratory in Duluth, Minnesota . AQUIRE data are extracted from literature published worldwide and from independently compiled data files . Documents to be abstracted are identified through online literature searches, from 17

## p . 10 7

review article and criteria document bibliographies, and from existing aquatic toxicity reprint collections . AQU IRE includes data on acute and chronic toxicity, bioaccumulation, and sublethal effects generated from tests performed on freshwater and saltwater species . Data on aquatic mammals, birds, and bacteria are not included in AQUIRE . The data are formatted into records at the level of the individual tests or observations . Each record contains chemical substance information, test organism details, study protocol, experimental details, and results for one test or one observation within a given reference document . Thus, there can be multiple AQUIRE records for a given chemical from a single paper as well as from several different papers . 7 . Phvtotox: Phytotox is a database dealing with the effects of organic chemicals on terrestrial vascular plants . The database has been compiled in the Department of Botany and Microbiology at the University of Oklahoma under sponsorship of the U .S . Environmental Protection Agency . All information in the database has been extracted from open literature . Each record in the database contains information about the effects of the application of one concentration of a single chemical on a particular plant species as reported in one paper . Papers selected for inclusion in Phytotox must satisfy three principal criteria : (1) a terrestrial plant was studied ; (2) organic chemicals were applied ; and (3) direct effects were evaluated . Data are then extracted from these papers for inclusion in the Phytotox database . Each record in the database defines the chemical and plant species involved in the test, provides dosage and application information, lists all noted effects of the test on the plant, and provides a bibliographic citation for the source of the data . 8 . Envirofate : Envirofate, developed through the collaborative efforts of EPA's Office of Toxic Substances and the Syracuse Research Corporation, contains data on approximately 800 chemicals . Envirofate contains summary information concerning the environmental fate and the physical-chemical properties of chemicals released into the environment . Chemicals selected for inclusion in the database are produced annually in excess of one million pounds . Envirofate contains twenty-four types of data extracted from papers published worldwide dealing with environmental fate and behavior studies
. Data Sources Available through TOXNET . a Division of the National Library of Medicine (N LM1 -- Call 1-800-638-8480 for information on TOXNET. 9 . Environmental Mutagen Information Center (EMIC) : EMIC is a bibliographic database on biological and physical agents and chemicals that have been tested for genotoxic activity . EMIC covers publications from 1991 to present ; earlier years are covered in EMICBACK . The files are maintained by federal funding . The database can also be searched online through the TOXNET system . URL : http ://sis .nim .nih .gov 10 . Environmental Teratology Information Center (ETIC) . also known as Develoomental and Rel2roductive Toxicology Database (DART) : ETIC is a bibliographic database containing citations to publications concerning developmental and reproductive toxicology . It covers 1989 to present ; earlier years are covered in ETICBACK . The files are maintained by federal funding . The database can also be searched online through the TOXNET system . URL : http ://sis .nim .nih .gov Libra Reference Boo k 11 . Shepard's Catalog of Teratogenic Agents (CTA1 : The CTA (81h edition) is a reference book published in 1995 by The Johns Hopkins University Press containing teratogenic information on more than 2500 chemicals . The book was authored by Thomas Shepard, and it provides a comprehensive compilation of animal and human research on the teratogenicity of chemical and environmental agents . The CTA includes many references for the Japanese as well as the American and European literature .



# 18

## p . 108

APPENDIX III . 

# Tables and Figures

19

## p . 109

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## p . 112

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## p . 113

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## p . 114

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## 25 .0 17 .9 16• 7 13 .4 15 .1 11 .4 10 .1 6 .5 7 .3 8 .4 7 .1 5 .9 3 .4



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## p . 115

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## p . 116

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Tibk tNuu/ lm AM lNky Ur U .S. lu o hdoaia 

# Yoiaat (flPY) (ftiai s Ttas PaferoN` i o aft of 9PY (bcmia6 0 in6 (4zS%) 1 395 (B .8%) 2 296(10.3%) 3 ibb (9.3%) 4 z51(9 .o%) S 231 ( M ) 6 202 (7.1%) 

# TOTAL i86 3 SNSaaunYduk~mnmddt►,~a+~► .~u~t,~1 .~1 .~rarYw~uli~te. hrkrd~er~NqnMiaiKMdtl,~eYtl~l,calwtlry' .~ra~kiwa~dtxeaamweeauYnd~nwkr~aoAnrd~r+~tnth~Yat ~ae Iqtd, n~ tk SqS ftt Fr dae eilNMd "Mat* a o k c 

### anso.~ fM ►w~o~ .~9.1 Nwa~lokkw~o~llu~a~a

## p . 118

Table 3 . Comparisons of Availability of Hazard Data for High Production Volume (HPV)

Chemicals to Other Studies :EDF 100 CMmkalr 4~ Cha»Icsl Hazard Data Tast Type ` : $tudyc" Tozk lynoran+cs '~ i~ tlraff Raport" . ~lvalk blHty Study' Acute Toxicity 20% >90% 99% 49% Repeated Dose Toxicity' 10% >75% 95% 14% (Subch ronic / Ch ronic) (10%/4%) (>75% / >40%) (Not Available 114%) Developmental Toxicity >55% 71% 8 

### Reproductive Toxicity 

## 6% 47% 5 

## 8 9(, 23 % Genetic Toxici ty (jn yj

W 93 % Genetic Toxici ty (In yjyQ) 9% >75% 71% 34o,6e Meets SIDS Requirements for 29% 47% 9% Human Health ' 'Based on results of study conducted by National Academy of Sciences National Research Council In 1984 ( NRCJNAS . 1984; pp.45-50) on 259 HPV chemicals randomly selected from the TSCA existi ng chemical inventory list (at that time, 48,523 chemicals), which underwent a standardized sc reening procedu re to identify publicly available toxicity information (i .e., additional test data may exist in restricted acoess files) . 'Chemicals with at least one test in each SIDS health category were oonsidered to meet SIDS requi re ment for that ceEegory . The six SIDS health categories a re acute toxicity, repeated dose toxicity, developmental toxicity, reproductive toxicity, genetic toxicity Qp yW , and genetic toxicity QQ yW . 'CMA, 1997 . `Based on EPA Information on 2863 U .S . HPV Chemicals from 1990 IUR Update. 'Test components in repeated dose toxicity tes ti ng element include subch ronic toxicity testing and ch ronic toxicity testing. 'For developmental/reproductive toxicity and mutagenicity , test data were conside red available if any studies re levant to the endpoint we re located ; completing the SIDS set for these endpoints requires multi ple studies. 'For EPA's Chemical Hazard Data Availabili ty study, SIDS human health testi ng Includes acute toxicity, chronic toxicity, developmental/reproductive toxicity, and mutagenici ty. For EDF and CMA analysis, SIDS human health data were available In each of the six toxicity endpoints listed above .

## p . 119

Tabk 4. Ilu ud Data ArnUi bitlq for O.S. No MdactW 

# velawc (NPV) TAI (Wfals No L' 

### q

-t~ 

# K - 4 lfl 141M11 # 

### TtuS ~ f d'~f~" `_ , ~tf b l 

## 0 0(0-0%) 1216 (61 %) 1 z (1 .0%) 393 (148%) 2 (i .S % ) 2 91 ( 1 o1 96) 3 (1 4%) 29 (9•4%) 4 z1(10 .3%) 5 ~ 4 1%) 180 (6 .8%) 6 loq ( g .T%) 93 (3d%) TOTAL 103 z66o wtuaeua~ar~xreu~ .anrcw~r,~w~r,~dn .~r,~~r~ . ►»~~r.•~+an.aua~aa,~a~r.n~wrx~mcba~a~na~r~rarkcrem~n,~ ~ Nee Ncxd; aipktlK tlk fYS ~t ~r u~ne e~~Yn rc~im M~k ~aHn ero1~a v~~d~n~~3u~~Yabraerr~~oN~¢Mm

## p . 120

Table 

# S. Tlu : Hazard 

# oaq Arnili

# biliq f 

# or O .S. Nish Produdo 

# YoW (NPY) TRI (hapiais : Toni Oasite and OfBite Ikka us ir faess of t MiIIioA Pouads ' Maa btr 4 Sm TN (bald 16 T " woo ad : Ttst3 P~t71tf ~ ;~ ~4 Od~tc AdoStS~ j 

# 0 0(0-0%)

~ 0(0-0%) 

# 2 00 .0%) 

# 3 1 

# (i. 1%

# ) 4 4 (44%) S 18 

# (tq.8%) 6 68 

# (141%) TOTAL 'iI dl anilt a dMftlotrdeaf t O ud taltt d mn adtnW bN

ppl. `ipfkrtf~Intlokx~cnoddq .drMkutldtr .l~d~na~hqM~e~~dtP .~l~1 .~A~luii~Wf+tt. hrdMd~hqnMphenddtp .~t~ddtP,c~tP,y~leNireMetAf~t,teaqhretadkrdmWkYalmNardm~tuthalpYt ~ertNatd;a~ktlKtlrflqkt~rdrftaipharq~ra~Ntl~k paAtt. Im IIfR EM H~rwtW M 21aj U~ IMY(hEBldl hlnq~011M Iqlxe

## p . 121

Tibk 6 . ON : Nord Data A►ailablliry for U .S. Kip Produtk 

# Volume (8PY) OSHA PEL (hemin h :qr~ba_ofADi ltahp >s = OSl1A Pfl fbaninl = Tctd ~a~nrM' ' '16 0 1 (o.s%) B 03 (48.8%) 1 q (0 ) 4b ( 4 1 %) 2 29 hs .o96) 38604 4%) 3 53474%) 314 (n.896) 4 101 (¢.3%) 14 10) TOTAL 1 93 267o I spKholnaamnYdrexMemiary,enrcmddq * dW a ** NWk u " ,ir M% e " . ferlnd~naW/rpnwnhetatlry~l~t~eldt ►,caranMeea~Yed~aN~lkr~aelktrekwxntlknpi~t~ereYdcknn~ktlKthSNll~tkrmaernqaUu r*1MM**ft

k Wdllare : D~ 16 Ub Mtt63U1INYUMbhAan99eIUIw

## p . 122

Tibic 1 . (K: Nuud qm ArnMGtp for O.S. Niih Indoai M 

# Yoiamc ( NPY) (ooumer Prodoa (heminls -- , , . tiimra hoh a (DcmiaF l~mbcr of .AB S Tcu~ ~afar~cC ~ ~ 0 32 (6'~%) 1 36(7.3%) 359 (v,' %) z SA (n .4%) 20(10.1%) 3 66(13.4%) zoo (8.4%) 4 88 (r1 .9%) ibq (1 .1 %) s qz ( 8 '1%) 13q M %) 6 tt i (46%) 810.4%) TOTAL 49 237z 9Ktarqardwexaras+lar.drMCaddry.Irld bpn IM wMqMmddq, mv4eidt►.coppdtr,sresriwMedt w- hrksd * eN+l/rq►Ms~hepoddq, Wt N ekltb mWxkr.yraNN Nsulpa.axpnMeearolled MNdkrNY smaa *W nthteW* Wrt Mo * W 1 * tk 1N! sG fr ewe a * Yn "ft sw * sdlks. `k Naoeta! Y EM's ~ f~ Ih~p~ I~aku. IiaAarc: flslYf~rs>pH~~s~G11MYUseYOkf~st~9oNll~pa .

## p . 12 3



# Table 8 . Estimated Costs for SIDS, SIDS+ and SIDS++ Testing Tiers

(Based on High Production Volume/Exposure ) Level I (SIDS Battery

)
Physical Chamkal Propa►~ ~~ > ~ 102 Melting Point CMA :600 $600 103 Boiling Point CMA :650 $600 104 Vapor Pressure CMA =1,550 $1,550 107 n-Octanol/Water Partition Coefficient (Shake Flask) CMA $3,750 $3,750 105 Water Solubility CMA $3,200 $3,200 EnvironmonW Fate and Pathways'- Photodegradation (Via Simple Estimation) CMA :2,600 $2,600 - Hydrolysis - Stability in Water (Via Estimation) CMA $3,100 $3,100

Transport/Distribution (Fugacity Model) CMA $750 :750 302 Inherent Biodegradation CMA :13,050 $13,050 Ecohaxlclty , 203 Acute Toxicity to Fish CMA $8,000 $8,000 202(1) Acute Toxicity to Daphnia CMA $6,100 $6,100 201 Toxicity to Aquatic Plants (Algae) CMA $7,250 $7,250 Mammalian ro"xich , 403 Acute Inhalation Toxicity OR CMA $15,900 $8,00 0 401 Acute Oral Toxicity OR CMA $4,500 (Avg of 403, 401, 402) 402 Acute Dermal Toxicity (870.1200) [EPA] [ ; 3,700] Mammalian Toxicity - Genotoxicit y 471 Genetic Toxicity (Ames-Salmonella typhimurium) CMA $5,150 $5,150 473 Chromosomal Aberration (in vitro) OR CMA ;19,500 :18,750 (Avg of 473 and 474) 474 Chromosomal Aberration (in vivo - micronucleus test) CMA $18,000

Mammalian Toxicity - Repeated DosolRaprolDevo l 422 Combined Repeated Dose w/ Repro/Devel Tox Screen CMA $122,000 $122,80 0 OR (Avg of 422 & (407+415)) 407 Repeated Dose Oral Toxicity & CMA ; 60,000 415/421 Reproductive Toxicity (1-generation) CMA $63,600 

## Total $ M 250

## p . 124



# Level II (SIDS + )
SIDS Battery (Level Q AND CMA ; 205,000 [approxt miata flyu re] $ n5i0q0 Shake Flask Die-Away Test ( 835 .3170) [EPA] $33,500 $33,500 Fish Biocentration Study (850.1730) [EPA] $47,200 [flow-thru] $47,200 Fish Early Life Stage [static or flow-th rough] ( 850.1400) [EPA] $40,000 [avg all species] $40,000 Daphnia Chronic Toxicity (850 .1300) [EPA] $28,200 [avg of staticlflow-thru] $28,200 Avian Dieta ry Study in Bowhites or Mallards ( 797 .2050) [EPA] $10,000 [avg both species] $10,000 Pharmacokinetics/ADME [Tier 1] (870 .7485) [EPA] :27,000 :27,000 Acute Neu rotoxicity ( 798 .6050-6200-6400) [EPA] $65,000 [avg all ro utes - rat] $65,000 Mouse Lymphoma Assay (798 .5300) [EPA] $15,000 $15,000 Dominant Lethal Assay [rodents] (798.5450) [EPA] $75,000 [avg all] $75,000 Subchronic Toxicity (798 .2250, 2450, 2650) [EPA] ;125,000 [avg all routes - rat] ;125,000 

## wl Immunotoxicity Satellite (SRBC - PFC or ELISA) [EPA] [TBD] [TBD] Subchronic Neu rotoxicity (79&60517-6200-6400) [EPA] $200,000 [avg all routes - rat] :200,000 Developmental Tox (2 species) ( 798.4900) ($70,000 X 2) [EPA] $140,000 [avg all] : 140,000 2-Generation Reproduction Study ( 870.3800) [EPA] : 450,000 [avg all routes - rat] $450,000 Total - i 1,500,000

Level III (SIDS++ ) 

# - SIDS Battery +(Lavels I and II) AND $1,500,000 ;1 ^ pp0 :.. : Sediment/Water Microcosm Biodegradation [EPA] $81,400 [avg of staticlflow-thru] $81,400 Fish Full Life Cyle Test (850.1500) [EPA] $250,000 [RAD/OPPT Est. - oral comm .] :250,000 Avian Reproduction Test in Mallards (797 .2150) [EPA] :66,400 [dieta ry route] $66,400 Pharmacokinetics ( Mechanistic) [EPA] [TBD] [variable cost] (TBD] Mouse Specific Locus Assay ( 798 .51951 5200) [EPA] $750,000 [avg visible/biochemical] $750,000 Heritable Translocation Assay ( 798 .5460) [EPA] :60,000 [avg all routes - mice] $60,000 Developmental Neurotoxicity (870 .6300) [EPA] : 150,000 [avg all routes -rat ] $150,000 Chronic/Onco [2-species ( rats and mice)] (870.4200) [EPA] $1,352,000 [avg all routes] : 1,352,000 Total $ 4,200,00 0 " CMA estimated testing costs taken from a table enclosed with a letter dated June 3,1997, from D . HeIin/CMA add ressed to C .

Auer/CCD/OPPTIEPA with regard to a CMA proposal for an OECD/SIDS "scoring" system . SIDS+ estimate of - : 1,500,000 does not include costs for immunotoxicity satellite in the subchronic toxicity stud y SIDS++ estimate of - : 4,200,000 does not include pharmacokinetics testing which is both chemical case specific and variable in cost NOTE : Positive effect(s) obse rved in lower tier test(s) would shift the chemical to higher testing tier(s) as needed .

All EPA source cost estimates a re from Economic and Policy Analysis BranchlEETD/OPPT (11/03 1 97 Data Cost Table and a 1998 (specifi c date unknown) Data Cost Table) unless otherwise specified in "Considerations"