Reproductive and Developmental Effects of Oral Exposure to Pentaerythritol Tetranitrate (PETN) (CASRN

78

- 11

- 5) in the Rat

Performed by U.S. Army Center for Health Promotion and Preventive Medicine for test sponsor Dyno

Nobel Inc.



## Responses to EPA’s Request for Additional Information

Mortality Data and Esophageal Perforations

-

All rats that were found dead or were euthanized prior to the study completion were from the 1000

mg/kg

- d dose group. All of these rats had evidence of esophageal perforatio ns caused by dosing

containing large amounts of compound and vehicle . Rat #441 was dosed a total of four days and died

prior to being paired with a female. Upon necropsy, corn oil was found in its lungs and an esophageal

perforation was noted. An unexpl ained cut along the length of its testes was also noted. Rat #452’ s

esophagus was so inflamed at the day of its euthanasia that it was noted that the gavage needle co uld

not reach the stomach. During necropsy corn oil was observed in a blind pocket of th e left axilla and an

esophageal perforation was observed. Rat #401 likewise was noted to have “caseous/purulent material

SQ (subcutaneous) in left axilla” with a perforated esophagus following euthanasia. Of the two rats that

were found dead, corn oil wa s noted in the peritoneal space along with an esophageal perforation for

#456 and only an esophageal perforation was noted for #417. All other remaining rats were euthanized

upon the completion of the study and no additional esophageal perforations were o bserved.

Requested Animal Data

– Please find tables as requested as follows.Table 1. Mating data per pair of rats exposed to PETN : date pairs were made (date paired), date sperm plug observed (sperm plug), number of sperm plugs

per p air (# plugs), female achieved pregnancy (pregnant), date of birth for pups (DoB), number of days pregnant (days pregnant), n umber of days from date

paired to date sperm plug observed (days to mate), number of males per litter (male pups), number of female s per litter (female pups), male/female ratio

(m/f ratio), comments. treatment female ID male ID date paired sperm plug # plugs pregnant DoB # pups DoD days pregnant days to mate male pups female pups m/f ratio comments

Control 386 427 26 Sep. 28-Sep 3 yes 21-Oct 9 25-Oct 23 2 5 4 1.25

Control 387 426 26 Sep. 28-Sep 3 yes 20-Oct 12 24-Oct 22 2 6 6 1.00

Control 390 435 27 Sep. 28-Sep 1 no NA 0 29-Oct 0 1 0 0 NA

Control 391 431 27 Sep. 28-Sep 4 yes 21-Oct 14 25-Oct 23 1 11 3 3.67 1 M & 1F died by d4

Control 394 430 27 Sep. 28-Sep 3 yes 21-Oct 14 25-Oct 23 1 4 10 0.40

Control 395 434 27 Sep. 28-Sep 3 yes 21-Oct 14 25-Oct 23 1 6 8 0.75

Control 402 443 28 Sep. 1-Oct 3 no NA 0 29-Oct 0 3 0 0 NA

Control 403 459 28 Sep. 2-Oct 2 yes 24-Oct 13 28-Oct 22 4 9 4 2.25 1 runt (F) FD d2; 1 F dead by d4

Control 418 458 28 Sep. 3-Oct 3 yes 25-Oct 13 29-Oct 22 5 8 5 1.60

Control 419 442 28 Sep. 29-Sep 2 yes 21-Oct 15 25-Oct 22 1 8 7 1.14

100 mg/kg 388 429 26 Sep. 28-Sep 5 yes 20-Oct 16 24-Oct 22 2 9 8 1.13 1 M stillborn

100 mg/kg 389 436 26 Sep. 29-Sep 2 yes 22-Oct 14 26-Oct 23 3 8 6 1.33

100 mg/kg 396 460 26 Sep. 27-Sep 5 yes 19-Oct 16 23-Oct 22 1 8 8 1.00 1 F died 2d after birth

100 mg/kg 397 428 26 Sep. 30-Sep 2 yes 22-Oct 16 26-Oct 22 4 8 8 1.00 1 M died by d4

100 mg/kg 420 461 27 Sep. 1-Oct 3 yes 23-Oct 14 27-Oct 22 4 8 6 1.33

100 mg/kg 421 437 27 Sep. 30-Sep 4 yes 22-Oct 14 26-Oct 22 3 4 10 0.40

100 mg/kg 422 462 27 Sep. 28-Sep 3 yes 20-Oct 15 24-Oct 22 1 9 6 1.50

100 mg/kg 423 463 27 Sep. 28-Sep 3 yes 21-Oct 11 25-Oct 23 1 6 5 1.20

100 mg/kg 424 465 28 Sep. 1-Oct 4 yes 23-Oct 16 27-Oct 22 3 9 7 1.29

100 mg/kg 425 464 28 Sep. 29-Sep 1 yes 21-Oct 19 25-Oct 22 1 10 9 1.11 2 M died by d4

500 mg/kg 392 432 26 Sep. 28-Sep 4 yes 20-Oct 10 24-Oct 22 2 7 3 2.33

500 mg/kg 393 433 26 Sep. 28-Sep 3 yes 20-Oct 11 24-Oct 22 2 6 5 1.20

500 mg/kg 398 438 26 Sep. 30-Sep 4 yes 23-Oct 10 27-Oct 23 4 4 6 0.67

500 mg/kg 399 439 26 Sep. 27-Sep 5 yes 19-Oct 14 23-Oct 22 2 5 8 0.63

500 mg/kg 408 448 27 Sep. 1-Oct 3 no NA 0 29-Oct 0 4 0 0 NA

500 mg/kg 409 449 27 Sep. 30-Sep 2 yes 22-Oct 19 26-Oct 22 3 9 11 0.82

500 mg/kg 410 454 28 Sep. 1-Oct 1 yes 23-Oct 14 27-Oct 22 3 8 6 1.33

500 mg/kg 411 451 28 Sep. 29-Sep 2 yes 21-Oct 10 25-Oct 22 1 6 10 0.60

500 mg/kg 414 455 28 Sep. 29-Sep 3 yes 21-Oct 17 25-Oct 22 1 12 5 2.40

500 mg/kg 415 450 28 Sep. 1-Oct 4 yes 23-Oct 14 27-Oct 22 3 7 7 1.00

1000 mg/kg 400 444 26 Sep. 28-Sep 3 yes 21-Oct 14 25-Oct 23 2 5 9 0.56

1000 mg/kg 404 440 27 Sep. 30-Sep 1 no NA 0 29-Oct 0 3 0 0 NA

1000 mg/kg 405 447 27 Sep. 28-Sep 3 yes 20-Oct 14 24-Oct 22 1 10 4 2.50

1000 mg/kg 406 445 27 Sep. 30-Sep 3 yes 22-Oct 15 26-Oct 22 3 9 6 1.50 1 M dead by d4

1000 mg/kg 407 446 27 Sep. 2-Oct 1 no NA 0 29-Oct 0 5 0 0 NA

1000 mg/kg 412 453 28 Sep. 1-Oct 3 yes 23-Oct 7 27-Oct 22 3 1 6 0.17

1000 mg/kg 413 457 28 Sep. 29-Oct 4 yes 21-Oct 15 25-Oct 22 1 8 7 1.14Table 2. Individual data for rats exposed to PETN : number of left corpora lutea (L CL), number of right

corpora lutea (R CL), number of left implantation sites (L IS), right implantation sites (R IS), total number

of corpora lutea (total CL), total number of implantation sites (total IS), number of live pups born (# live ),

number of stillborn (# dead), calculated pre

- an d post

- implantation losses. *The one pup that was found

dead on the day of its birth (perhaps stillborn) had a laceration on its throat.

treatment female ID R CL L CL R IS L IS total CL total IS # live # dead pre-implant loss post-implant loss

Control 386 7 7 7 7 14 14 9 0 0 5

Control 387 9 11 8 5 20 13 12 0 7 1

Control 390 0 0 0 0 0 0 0 0 0 0

Control 391 8 5 9 5 14 14 14 0 0 0

Control 394 10 6 9 6 16 15 14 0 1 1

Control 395 7 9 7 9 16 16 14 0 0 2

Control 402 0 0 0 0 0 0 0 0 0 0

Control 403 6 10 5 10 16 15 13 0 1 2

Control 418 8 6 8 6 14 14 13 0 0 1

Control 419 15 11 9 7 26 16 15 0 10 1

100 mg/kg 388 10 16 7 11 26 18 16 1* 8 3

100 mg/kg 389 9 7 9 7 16 16 14 0 0 2

100 mg/kg 396 10 8 9 8 18 17 16 0 1 1

100 mg/kg 397 5 11 5 11 16 16 16 0 0 0

100 mg/kg 420 10 8 7 8 18 15 14 0 3 1

100 mg/kg 421 4 11 4 11 15 15 14 0 0 1

100 mg/kg 422 7 10 7 9 17 16 15 0 1 1

100 mg/kg 423 7 8 7 8 15 15 11 0 0 4

100 mg/kg 424 10 6 10 6 16 16 16 0 0 0

100 mg/kg 425 9 11 9 11 20 20 19 0 0 1

500 mg/kg 392 8 8 6 6 16 12 10 0 4 2

500 mg/kg 393 9 4 8 4 13 12 11 0 1 1

500 mg/kg 398 11 10 7 5 21 12 10 0 9 2

500 mg/kg 399 12 5 9 5 17 14 14 0 3 0

500 mg/kg 408 0 0 0 0 0 0 0 0 0 0

500 mg/kg 409 10 10 10 10 20 20 19 0 0 1

500 mg/kg 410 7 11 6 10 18 16 14 0 2 2

500 mg/kg 411 6 10 6 10 16 16 10 0 0 6

500 mg/kg 414 4 13 4 13 17 17 17 0 0 0

500 mg/kg 415 7 10 7 7 17 14 14 0 3 0

1000 mg/kg 400 4 11 3 11 15 14 14 0 1 0

1000 mg/kg 404 0 0 0 0 0 0 0 0 0 0

1000 mg/kg 405 11 4 11 4 15 15 14 0 0 1

1000 mg/kg 406 8 8 7 8 16 15 15 0 1 0

1000 mg/kg 407 0 0 0 0 0 0 0 0 0 0

1000 mg/kg 412 7 8 7 8 15 15 7 0 0 8

1000 mg/kg 413 9 8 9 8 17 17 15 0 0 2Table 3. Organ weights from male and female Sprague

- Dawley rats orally exposed to PETN. treatment male ID bw testes epididymis female ID bw ovaries



### control 427 497 3.604 1.258 386 282 0.116

control 426 433 3.375 1.314 387 281 0.102

control 435 462 3.638 1.222 390 301 0.113

control 431 433 3.775 1.604 391 248 0.12

control 430 515 3.669 1.576 394 312 0.131

control 434 452 3.569 1.366 395 301 0.11

control 443 534 3.405 1.227 402 300 0.087

control 459 555 3.375 1.417 403 368 0.125

control 458 482 3.674 1.429 418 301 0.123

control 442 532 3.412 1.219 419 321 0.131

100 mg/kg 429 484 3.803 1.579 388 318 0.128

100 mg/kg 436 474 3.584 1.307 389 325 0.079

100 mg/kg 460 446 3.531 1.175 396 318 0.134

100 mg/kg 428 535 3.572 1.57 397 341 0.156

100 mg/kg 461 476 3.822 1.343 420 317 0.147

100 mg/kg 437 470 3.316 1.292 421 324 0.135

100 mg/kg 462 450 3.452 1.255 422 328 0.122

100 mg/kg 463 483 3.332 1.382 423 348 0.117

100 mg/kg 465 519 3.436 1.276 424 336 0.146

100 mg/kg 464 474 3.09 1.274 425 341 0.152

500 mg/kg 432 460 3.375 1.241 392 355 0.159

500 mg/kg 433 481 3.175 1.395 393 324 0.124

500 mg/kg 438 462 3.464 1.682 398 321 0.126

500 mg/kg 439 503 3.696 1.528 399 348 0.228

500 mg/kg 448 518 3.255 1.217 408 321 0.177

500 mg/kg 449 505 3.566 1.207 409 340 0.114

500 mg/kg 454 492 3.425 1.387 410 312 0.123

500 mg/kg 451 459 3.092 1.105 411 325 0.134

500 mg/kg 455 442 3.999 1.361 414 329 0.131

500 mg/kg 450 489 3.311 1.46 415 342 0.14

1000 mg/kg 444 474 3.784 1.574 400 340 0.122

1000 mg/kg 440 452 3.635 1.282 404 252 0.126

1000 mg/kg 447 482 3.507 1.288 405 291 0.133

1000 mg/kg 445 494 3.304 1.057 406 311 0.114

1000 mg/kg 446 441 3.429 1.139 407 314 0.131

1000 mg/kg 453 445 3.208 1.132 412 313 0.151

1000 mg/kg 457 547 3.531 1.443 413 346 0.131

1000 mg/kg 416 268 0.105Table 4 . Number of litters produced per pair for each treatment level of rats exposed to PETN. treatment number of litters

Control 8/10

100 mg/kg 10/10

500 mg/kg 9/10

1000 mg/kg 5/7

dose female

ID F1 BW F4 BW M1 BW M4 BW Clinical Obervations/Gross Abnormalities

0 387 5.8 9.5 6.1 9.8 All pups NGLR

5.9 9.9 6.6 9.7

5.7 9.1 5.9 9.9

6.1 9.9 5.8 9.9

6.2 9.6 6.3 10.8

5.8 9.8 5.9 10

0 386 6.4 11.5 6.7 12.2 M

- left testis & epididymis slightly hemorrhagic

6.5 11.6 7 12.6 All other pups NGLR

7.2 10.5 7.1 11.8

6.9 11.6 7.5 11.5

6.9 11.9

0 419 5.7 8.6 6 8.5 All pups NGLR

5.9 9.5 6.2 10.3

5.7 9.4 6.3 10.5

5.4 10.2 5.7 9.4

5.7 10.1 6 11

5.8 8.8 6.4 9.2

5.7 9.8 6.1 8.7

5.6 11

0 391 5.6 6.9 5.9 7.4 All pups NGLR

5.5 7 6 7.7

5.5 5.8 7.8

5.9 7.2

5.2 7.3

5.9 6.9

5.6 7.5

5.9 6.8

5.4 7.2

4.7 7.2

6

0 395 6.2 10.5 6.7 9.9 All pups NGLR

6.4 9.8 6.7 11

6.2 9.7 6.3 9.96.5 10 7.4 11.1

6.5 10.4 6.9 10.3

6.6 10.1 6.9 9.9

6.8 9.5

6.8 10.5

0 394 6.9 11.8 6.9 11.1 All pups NGLR

6.4 11.1 7.1 12

6.7 10.8 7.2 11.2

6.5 11.1 7.2 12.2

7 12.2

6.8 11.5

6.7 11.3

6.8 11.1

6.7 12.1

6.8 11.3

0 403 4.5 10.5 6.7 10.5 All pups NGLR

6.1 10.3 6.1 10.8

5.6 6.5 11

5.7 6.7 11.9

6 11.3

6.1 11.5

6.4 10.7

6.1 11.6

6.7 10.4

0 418 4.9 9.5 5.2 9.2 M

- hematoma on left testis

5.7 9.3 5 10.5 M

- hematoma on right testis

5.1 9.6 5.4 8.6 All other pups NGLR

5.5 9.3 5.1 10

6 8.6 6 10.5

5 9.9

5.3 9.3

4.9 8.6

100 396 6.2 9.8 5.8 10.3 F

- mottled liver

5.5 9.8 5.6 9.5 F

- mottled liver

6.2 9.7 6.3 9.9 F

- mottled liver

5.8 8.7 5.6 9.4 M

- mottled liver

6.3 10.4 6.5 11.9 All other pups NGLR

6 10.6 6.6 9.6

5.8 9.6 6.7 9.6

6.5 6.4 9.4

100 388 5.2 9 6.3 9.9 All pups NGLR

6.3 7.6 6.3 9.7

5.9 9.7 5.2 9.16.6 8.2 6.1 9.7

6.4 9.4 5.8 9

5.5 10.4 6.3 9.5

6 9.8 6.1 10.1

5.6 9 5.3 9.8

6.4

100 422 6.1 10.4 6.1 10.2 All pups NGLR

6 9.8 6.3 10.1

5.7 9.3 6.5 10

5.5 9.8 5.9 10.5

5.9 9.5 6 10.5

6.1 9.3 6 10.5

6.4 10.3

6.2 10.7

6.3 11.1

100 423 7.3 13.9 8.6 12.3 All pups NGLR

8.4 13.3 8.5 12.9

7.9 13 8 14.1

8 12.5 8.5 13.7

7.7 12.3 7.5 13.8

7.3 13.2

100 425 6 9 5.6 9.3 All pups NGLR

6 9.1 6 9.4

5.2 8.1 6.2 9.5

5.5 7.9 5.8 10

5.4 9.7 5.7 9.8

6.1 8.7 5.5 7.7

5.9 9.1 5.9 9.9

5.2 8.4 6 9.6

5.8 9.2 5.8

6.2

100 389 7.9 10.4 8.1 11.4 M

- hematoma on L testis

7 10.9 6.3 12.1 All other pups NGLR

6.8 11.5 7.7 9.7

6.8 11.5 7.5 12.2

7.5 11.1 6.6 11.4

6.6 12.5 7.6 10

7.6 11.7

7.8 12.6

100 397 6.3 9.7 6.7 10.9 M

- hematoma on L testis

5.2 9.3 5.6 9 All other pups NGLR

6.2 9.6 6.3 8.9

5.7 9.2 6.6 10.25.8 11.5 6.5 9.7

6 10.3 6.4 10.6

6.2 11.2 6.7 10.4

6.5 10.1 5.4

100 421 7.8 11 7.6 10.3 All pups NGLR

7.6 10.7 7.6 10.6

7.8 10.8 7.8 10.7

8 11.1 7.9 10.4

6.7 9.9

7.1 10.9

7.8 10

7.7 10.3

7.2 10.4

7.6 11

100 424 7.2 9.7 6.9 9.3 M

- only 1 testis located

6.5 10.7 6.7 10.4 All other pups NGLR

6.4 10.4 6.2 10

5.5 9.7 6.3 10.9

6.4 8.7 6.9 9.8

6.5 10.4 7.1 10.3

6.6 8.5 6.8 10.6

6.4 10.9

6.7 10.5

100 420 6.4 10.6 6.5 11 All pups NGLR

5.8 10.9 6.9 11.4

6.6 10 5.9 11.1

6.5 10.2 6.8 9.9

6.7 10.2 6.8 11.1

6.3 10.2 6.7 10.1

6.7 10.7

6.4 10.6

500 399 6.4 10.8 6.4 10.5 M

- mottled liver

5.9 9.4 6.6 10.6 M

- mottled liver

6.1 10.9 6.4 11 F

- mottled liver

6.6 9.3 6.4 10.8 All other pups NGLR

6.5 9.8 6.8 10.4

6.1 10.1

5.9 10.5

6.1 10.3

500 393 6.9 10.7 7 11.4 M

- enlarged spleen

6.3 10.4 6.6 11 All other pups NGLR

6.5 11.6 6.6 11.2

6.6 10.3 7 10.37.2 11.5 6.9 11

6.8 10.2

500 392 6.1 12 6.9 10.2 All pups NGLR

6.4 11.8 6.3 11.6

6 11.3 6.7 11.4

6.4 11.6

5.6 12.5

6 11.7

6.3 11.9

500 414 5.9 9.1 6.6 10.6 All pups NGLR

5.9 9.2 6.3 9.9

5.7 8.8 6.5 10.8

6.6 8.7 6.6 8.9

6.4 11.3 6.8 9.4

6.4 9.5

5.8 11.2

6.6 7.7

6.6 9.1

6.2 10

6.4 10.7

7 10.4

500 411 7 9.7 7.1 10.9 All pups NGLR

5.9 10.1 7.2 10.9

6.6 9.7 7.4 9.9

6.7 11.4 6.4 10.8

6.3 10.7 7 10.6

7 10.6 6.3 10.1

6.8 9.9

6.4 10.3

6.2 10.9

5.8 10.8

500 409 5.6 9.1 6.2 9.7 All pups NGLR

5.6 8.9 6.3 8.1

5.6 8.4 6.3 10.2

6.1 8.9 5.8 9.8

5.8 8.5 5.5 10.1

5.5 8.6 6 9.4

5.3 9 5.7 9.5

5.5 9.7 5.4 9.7

5.5 8.8 6.5 10

5.5 9.6

5.6 9.1

500 398 8.2 12.2 8.4 13.5 All pups NGLR7.4 13.6 9 13.4

8.4 13.6 7.4 12.9

8.1 13.1 8.3 12.3

8 13.3

8.1 13.6

500 415 5.9 9 6.3 10 All pups NGLR

5.1 10 6.3 9.5

6.4 9.7 5.9 9.8

5.4 8.2 6.3 9.4

6.3 9.7 6 10.1

6.2 8.8 6.6 9.6

6 9.5 6.1 9.4

500 410 6.3 11 6.5 10.5 All pups NGLR

6.6 10.8 6.9 9.2

6.4 10.6 6.7 10.9

6.8 10.5 6.9 11

6.3 10.5 6.8 11.6

6 10.3 6.8 11.1

6.8 11.1

7.5 11.8

1000 413 5.9 9.7 6.5 10.7 All pups NGLR

5.7 9.6 6.6 10

5.8 11.1 6 9.9

5.2 9.4 6 10.5

6.2 10.6 5.9 11

6.3 9.2 6.2 10.8

6.7 9.4 6.3 10.3

5.8 10.4

1000 400 6.6 12.2 7.8 11.3 F

- left atrial hematoma

7.8 11.9 5.8 11.9 All other pups NGLR

6.7 11.3 7.7 12.7

7.1 12.8 6.8 12.5

6.6 12 7.3 10.1

7.4 11

6.8 11.1

6.7 11.7

7.4 11.5

1000 405 6.8 8.9 5.9 9.3 All pups NGLR

5.5 9.3 5.7 9.3

5.8 9.2 6.3 9.6

5.8 8.5 6 9.2

6.2 9.2

6 10.2Table 5

. Pup body weights (BW; g) by dam (female ID): female body weights at day 1 (F1 BW), female

body weights at day 4 (F4 BW), male body weights at day 1 (M1 BW), male body weights at day 4 (M4

BW). Unless otherwise noted, missing data represent pups that wer e unable to be located and were

assumed to be dead /e aten by the dam. 6 9

5.8 9.3

5.5 9.3

5.8 9.2

1000 406 5.9 9.3 6.5 9.3 All pups NGLR

5.6 8.8 5.5 10.4

5.2 8.5 5.7 10.9

5.4 9.2 6.3 10.1

5.7 10.1 5.5 9.1

5.7 9.3 6.1 9

5.6 9.3

6.1 10.3

5.2

1000 412 6.6 12.1 7.3 13 All pups NGLR

6.5 12.3

6.6 12.4

6.5 12.1

7 12.6

6.4 12.5Table 6 . Adult male body weights (g) from Sprague

- Dawley rats that were orally exposed to PETN

. treatment male ID day 0 day 7 day 14 day 21 day 28 day 34

control 427 335 366 381 402 419 437

control 426 345 389 420 450 478 499

control 435 358 396 429 462 499 516

control 431 339 371 389 396 422 433

control 430 341 377 402 400 437 452

control 434 361 392 411 426 444 462

control 443 358 398 439 462 497 532

control 459 380 425 466 482 512 534

control 458 364 396 427 422 455 482

control 442 416 441 479 491 524 555

100 mg/kg 429 373 436 460 489 511 537

100 mg/kg 436 362 402 425 432 464 482

100 mg/kg 460 352 388 420 442 457 474

100 mg/kg 428 357 391 420 428 445 470

100 mg/kg 461 348 369 390 403 436 446

100 mg/kg 437 362 404 412 418 456 476

100 mg/kg 462 351 386 401 424 426 450

100 mg/kg 463 362 412 435 437 466 483

100 mg/kg 465 362 399 434 390 453 474

100 mg/kg 464 378 424 460 427 494 519

500 mg/kg 432 343 370 393 402 425 459

500 mg/kg 433 363 400 422 433 457 485

500 mg/kg 438 365 395 419 433 450 467

500 mg/kg 439 365 405 439 457 485 505

500 mg/kg 448 355 421 449 471 490 518

500 mg/kg 449 361 413 444 473 489 505

500 mg/kg 454 353 415 445 459 471 489

500 mg/kg 451 368 387 417 417 437 459

500 mg/kg 455 367 404 433 450 472 492

500 mg/kg 450 373 394 410 415 431 442

1000 mg/kg 400 340 371 383 394 432 462

1000 mg/kg 441 355

1000 mg/kg 404 366 397 417 426 450 470

1000 mg/kg 405 363 386 416 436 479 496

1000 mg/kg 406 377 397 420 400 415 441

1000 mg/kg 407 354 390 423 451 464 482

1000 mg/kg 452 367 390 366

1000 mg/kg 412 344 372 388 401 423 445

1000 mg/kg 456 358 399 348

1000 mg/kg 413 378 426 459 480 514 547Table 7 . Adult female body weights from Sprague

- Dawley rats that were orally exposed to PETN. treatment female ID day 0 day 7 day 14 day 21 post-partum

control 386 231 230 233 253 283

0 387 215 217 224 250 286

0 390 219 224 241 259

0 391 213 206 210 230 229

0 394 221 238 242 266 285

0 395 214 222 235 279 282

0 402 227 240 245 267

0 403 242 246 258 278 340

0 418 226 226 223 231 278

0 419 237 245 253 272

100 mg/kg 388 217 227 228 276 290

100 mg/kg 389 226 217 247 266 300

100 mg/kg 396 209 213 230 265 275

100 mg/kg 397 226 246 239 279 314

100 mg/kg 420 220 234 240 276 287

100 mg/kg 421 217 226 230 267 299

100 mg/kg 422 213 225 241 273 284

100 mg/kg 423 229 243 271 292 321

100 mg/kg 424 245 251 275 287 314

100 mg/kg 425 218 229 254 290 293

500 mg/kg 392 224 227 243 278 329

500 mg/kg 393 213 228 246 274 313

500 mg/kg 398 217 230 235 269 286

500 mg/kg 399 214 246 246 284 325

500 mg/kg 408 235 255 261 299

500 mg/kg 409 205 225 246 265 291

500 mg/kg 410 232 226 247 274 289

500 mg/kg 411 217 220 218 291 302

500 mg/kg 414 214 225 242 286 303

500 mg/kg 415 242 249 273 289 321

1000 mg/kg 400 218 244 253 277 294

1000 mg/kg 404 220 214 225 241

1000 mg/kg 405 222 224 239 265 283

1000 mg/kg 406 211 217 237 256 286

1000 mg/kg 407 225 242 259 272

1000 mg/kg 412 207 220 239 255 297

1000 mg/kg 413 243 253 259 293 311Table 8

. Weekly a dult male feed consumption data from Sprague

- Dawley rats orally exposed to PETN

. Missing values are circumstances when ca ge

- mate s were found dead or euthanized during/between

periods of feed consumption measurements

. 

### feed consumption (g)

treatment ID day 0-7 day 7-14 day 28-34

control 426/427 147 136 105

control 430/431 154 138 110

control 434/435 145 140 101

control 442/443 168 162 123

control 458/459 173 157 111

100 mg/kg 428/429 213 209 186

100 mg/kg 436/437 210 192 166

100 mg/kg 460/461 191 166 162

100 mg/kg 462/463 191 154

100 mg/kg 464/465 200 210 176

500 mg/kg 432/433 180 165 147

500 mg/kg 438/439 182 172 145

500 mg/kg 448/449 186 177 147

500 mg/kg 450/451 178 180 138

500 mg/kg 454/455 169 167 135

1000 mg/kg 440 137 122

1000 mg/kg 444/445 145 135 105

1000 mg/kg 446/447 142 134 99

1000 mg/kg 452/453 89 111

1000 mg/kg 456/457 157 125 67Table 9 . Adult female feed consumption encompassing paired, pregnant, and lactation periods from

Sprague

- Dawley rats orally exposed to PETN . Values in the day 0

- 7 and day 7

- 14 columns that are

adjacent to one another and identical are halved feed consum ption values that wer e derived from a

value from pair ed of rats. DoE = date of euthanasia. feed consumption (g)

treatment ID day 0-7 day 7-14 day 28-35 day 35- day 42- DoE

control 386 73 78 126 122 25 43

control 387 73 78 128 115 42

control 390 67 73 83 83 56 46

control 391 67 73 105 59 42

control 394 81 96 111 151 42

control 395 81 96 163 126 42

control 402 89 98 94 90 45 45

control 403 89 98 143 155 79 44

control 418 79 73 104 115 115 45

control 419 79 73 41

100 mg/kg 388 122 123 183 217 42

100 mg/kg 389 122 123 206 183 95 44

100 mg/kg 396 119 127 179 41

100 mg/kg 397 119 127 195 203 111 44

100 mg/kg 420 127 124 184 181 95 44

100 mg/kg 421 127 124 193 204 52 43

100 mg/kg 422 124 137 183 41

100 mg/kg 423 124 137 108 243 42

100 mg/kg 424 129 137 188 204 50 43

100 mg/kg 425 129 137 193 41

500 mg/kg 392 98 111 165 192 42

500 mg/kg 393 98 111 170 214 42

500 mg/kg 398 104 134 131 154 120 45

500 mg/kg 399 104 134 210 41

500 mg/kg 408 119 131 113 127 84 46

500 mg/kg 409 119 131 179 207 55 43

500 mg/kg 410 102 122 146 178 46 43

500 mg/kg 411 102 122 202 41

500 mg/kg 414 117 127 200 41

500 mg/kg 415 117 127 171 199 51 43

1000 mg/kg 400 93 78 133 147 42 43

1000 mg/kg 401 93 78 16

1000 mg/kg 404 74 91 83 93 59 46

1000 mg/kg 405 74 91 135 41

1000 mg/kg 406 86 95 134 153 43

1000 mg/kg 407 86 95 125 119 67 46

1000 mg/kg 412 92 101 145 123 32 43

1000 mg/kg 413 92 101 162 41

1000 mg/kg 416 76 83 82 41 45

1000 mg/kg 417 76 14