### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 2 of 37



### STATEMENT OF NO DATA CONFIDENTIALITY CLAIMS

No claim of confidentiality is made for any information contained in this study on the basis of its falling within the scope of FIFRA Section 10 (d) (1) (A), (B), or (C).

Sponsor Tiarco Chemical 1300 Tiarco Drive, Dalton, Ga 30720

Signature ___________________________

Sponsor/Submitter

Date ____________________________

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 6 of 37



### CONTENTS
PAGE N° TITLE PAGE

............................................................................................................................................. 1 STATEMENT OF NO DATA CONFIDENTIALITY CLAIMS

.............................................................. 2 STATEMENT OF GLP COMPLIANCE

.................................................................................................. 3 STATEMENT OF COMPLIANCE WITH GOOD LABORATORY PRACTICE

.................................. 4 STATEMENT OF QUALITY ASSURANCE

.......................................................................................... 5 CONTENTS

.............................................................................................................................................. 6 PERSONNEL INVOLVED IN THE STUDY

.......................................................................................... 7 SUMMARY

............................................................................................................................................... 8 1. INTRODUCTION

............................................................................................................................... 9 1.1 Study Objective

......................................................................................................................... 9 1.2 Study Guidelines

....................................................................................................................... 9 1.3 Test Facility and Study Period

.................................................................................................. 9 1.4 Archives

..................................................................................................................................... 9 2. EXPERIMENTAL PROCEDURE

................................................................................................... 10 2.1 Test Item

.................................................................................................................................. 10 2.2 Instrument

................................................................................................................................ 10 2.3 Principle

................................................................................................................................... 10 2.4 Methodology

........................................................................................................................... 11 2.4.1 Determination of Vapour Pressure [Effusion Method using TGA]

........................... 11 2.4.2 Calculation

................................................................................................................. 11 3. RESULTS

......................................................................................................................................... 12 3.1 Vapour Pressure

........................................................................................................................ 12 4. REFERENCES

.................................................................................................................................. 13 TABLES 1. Calculation of Vapour Pressure (Replication 1)

......................................................................... 14 2. Calculation of Vapour Pressure (Replication 2)

......................................................................... 15 3. Calculation of Vapour Pressure (Replication 3)

......................................................................... 16 4. Calculation of Vapour Pressure

.................................................................................................. 17 FIGURES 1. Reciprocal Temperature (1/K) Vs Log 10 

### [Vp (Pa)] (Replication 1)

........................................... 18 2. Reciprocal Temperature (1/K) Vs Log 10 

### [Vp (Pa)] (Replication 2)

........................................... 19 3. Reciprocal Temperature (1/K) Vs Log 10 

### [Vp (Pa)] (Replication 3)

........................................... 20 APPENDICES 1. Thermographs

............................................................................................................................. 21 2. Signed Study Plan and Study Plan Amendments

...................................................................... 24 3. Certificate of Analysis of Sodium 3-Nitrobenzene Sulfonate

.................................................... 35 4. GLP Endorsement of Compliance

.............................................................................................. 36

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 8 of 37



### SUMMARY

This study was conducted to determine the vapour pressure of sodium 3-nitrobenzene sulfonate (CAS # 127-

68 -4, US EPA HPV 3). The test item was supplied by Tiarco Chemical
, 1300 Tiarco Drive, Dalton, Ga 30720. The method followed was as per the guidelines of the OECD N° 104 (March 23, 2006) and U.S. EPA OPPTS 830.7950 (August 1996) and test method ASTM E 1782-08 (March 01, 2008).

The vapour pressure of sodium 3-nitrobenzene sulfonate at 25 °C was 1.07 × 10 -3 

### Pa.

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 9 of 37



### 1. INTRODUCTION 1.1 Study Objective This study was performed to determine the vapour pressure of sodium 3-nitrobenzene sulfonate (CAS

# 127- 68 -4, US EPA HPV 3). The study was conducted in compliance with the OECD Principles of GLP (1998).
1.2 Study Guidelines The present study was conducted according to the following guidelines:
OECD, 2006: OECD N° 104, “Vapour Pressure”. The Organisation for Economic Cooperation and Development (OECD) guidelines for the Testing of Chemicals, adopted by the Council on March 23,

2006.
Standard Test Method for Determining Vapour Pressure by Thermal Analysis, ASTM E 1782-08, approved March 1, 2008.
U.S. EPA, 1996: OPPTS 830.7950 “Vapour Pressure” (EPA 71 2-C- 96 -043). The United States Environmental Protection Agency, Product Properties Test Guidelines (August 1996).
1.3 Test Facility and Study Period This study was performed at the Department of Chemistry, Jai Research Foundation, Valvada - 396 108, Dist. Valsad, Gujarat, India.
Study Initiation : February 24, 2012 Experiment Start : October 04, 2012 Experiment Completion : October 04, 2012 Study Completion : November 07, 2012

1.4 Archives All original raw data including any storage medium for electronically recorded data, documentation, thermographs, the signed study plan
, the study plan amendments
, the draft report, a copy of final report and the representative sample of the test item will be retained in the GLP Archives at Jai Research Foundation for a period of ten years. At the end of this period, the Sponsor's instructions will be sought to either extend the archiving period or return the archived material to the Sponsor or for the material to be disposed of.

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 10 of 37

2. EXPERIMENTAL PROCEDURE
2.1 Tes t Item Details of the test item provided by the Sponsor (Ref. TIDS) are as follows:

Test Item Name
IUPAC Name
C.A. Name
CAS N°
Batch N° Analysed Purity Manufacture by Supplied by
Date of Manufacture Date of Expiry Appearance Sodium 3

- nitrobenzene sulfonate

Benzenesulfonic acid, 3

- nitro, sodium salt (1:1) Benzenesulfonic acid, 3

- nitro, sodium salt (1:1)
127

- 68

- 4
50623
96.9% ( Refer Certificate of Analysis, Appendix 3 )
Tiarco Chemical
Tiarco Chemical September 08, 2011 September 08, 2013 Yellow to tan granular Storage Condition (at JRF) As per the instruction received from the Sponsor on storage of the test item,

the test item was stored in its original container as supplied by the Sponsor at room temperature in the Test Item Control Office (TICO). The stabili ty of the test item in storage is the responsibility of the Sponsor.

2.2 Instrument TGA : Perkin Elmer, model Pyris 6 TGA
2.3 Principle The quantity of test item leaving a cell per unit time through an aperture of known size is determined under vacuum conditions such that return of substance into the cell is negligible (by measuring the weight loss).

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 11 of 37

2.4 Methodology 2.4.1 Determination of Vapour Pressure [Effusion Method using TGA] A quantity of 24.3396
, 29.2884 and 28.2148 mg test item was added to the balance pan of the instrument for replications I, II and III, respectively. The balance pan was allowed to stand for sometime to get stabilized. After the balance was stabilized, the reading was recorded electronically. The temperature scanning was started from 35 to 45 °C at a rate of 1 °C/minute using nitrogen as purging gas @ 50 mL/min. The mass (mg) and the temperature reading (°C) were recorded automatically into a computer file and printed. The readings of temperature and mass were converted into Kelvin (K) and kilogram (kg), respectively. The experiment was performed in triplicate and the mean value was reported.

2.4.2 Calculation The vapour pressure was calculated at each set temperature using the following formula:

 m  g Vp = ----------

A Where, Vp = Vapour pressure (Pa)  m = Mass difference (kg) g = Acceleration due to gravity (9.813 m/s 2 

### ) A = Area of the orifice (3.14 × 10 -6 

### m 2 

### ) After calculating the vapour pressure (Pa), the Log 10 

### value was calculated.
A graph for reciprocal temperature (1/K) versus Log 10 

### [Vp(Pa)] was plotted. The correlation coefficient, intercept and slope were calculated.
The vapour pressure at 25 °C was calculated using the following formula: Slope Log 10 

### [Vp(Pa)] =

-------------------- + Intercept

Temperature (K)
After calculating the Log 10 

### [Vp(Pa)] at the particular temperature, the antilog of the mean of the replications was calculated and reported.
The vapour pressure of the sample was measured over a range of temperature to enable extrapolation

to 29 8.15 K.

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 12 of 37



### 3. RESULTS 3.1 Vapour Pressure The Log 10 

### [Vp(Pa)] of sodium 3-nitrobenzene sulfonate (CAS # 127- 68 -4, US EPA HPV 3) for replications I, II and III was -2.95, -3.04 and -2.92, respectively (Refer Tables 1, 2 and 3). The mean Log 10 

### [Vp(Pa)] was - 2. 97. The vapour pressure of sodium 3-nitrobenzene sulfonate (CAS # 127- 68 -4, US EPA HPV 3) at 25 °C was 1.07 × 10 -3 

### Pa (Refer Table 4).

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 13 of 37



### 4. REFERENCES OECD, 1998: OECD Series on Principles of Good Laboratory Practice and Compliance Monitoring, Number 1, “OECD Principles on Good Laboratory Practice” ENV/MC/CHEM(98)17 (as revised in 1997).

OECD, 2006: OECD N° 104, “Vapour Pressure”. The Organisation for Economic Cooperation and Development (OECD) guidelines for the Testing of Chemicals, adopted by the council on March 23,

2006.

Standard Test Method for Determining Vapour Pressure by Thermal Analysis, ASTM E 1782-08, approved March 1, 2008.

U.S. EPA, 1996: OPPTS 830.7950 “Vapour Pressure” (EPA 712 -C- 96 -043). The United States Environmental Protection Agency, Product Properties Test Guidelines (August 1996).

Vohra.Y, 2006: Validation of Effusion Method for Determination of Vapour Pressure of Ethofumesate Technical, JRF Study Number 5874, Jai Research Foundation, Valvada - 396 108, Gujarat, India.

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 14 of 37



### Vapour Pressure of Sodium 3-Nitrobenzene Sulfonate (CAS # 127-68-4, US EPA HPV 3)
TABLE 1 Calculation of Vapour Pressure (Replication 1)

Tempera

ture (°C) Tempera

ture (K) Reciprocal of

Temperature (1/K) Mass (mg)

of Test Item Mass Difference (mg) Mass

Difference (kg)  m Vapour

Pressure (Pa) Vp Log 10 Vp (Pa) Log 10 [Vp(Pa)] at 2 5 °C

35.00 308.15 0.003245173 24.3396

- - - -

- 2.95 36.00 309.15 0.003234676 24.3341 0.0055 0.0000000055 0.017188376

- 1.76
37.00 310.15 0.003224246 24.3292 0.0104 0.0000000104 0.032501656

- 1.49
38.00 311.15 0.003213884 24.3249 0.0147 0.0000000147 0.045939841

- 1.34
39.00 312.15 0.003203588 24.3200 0.0196 0.0000000196 0.061253121

- 1.21
40.00 313.15 0.003193358 24.3132 0.0264 0.0000000264 0.082504204

- 1.08
41.00 314.15 0.003183193 24.3062 0. 0334 0.0000000334 0.104380318

- 0.98
42.00 315.15 0.003173092 24.2993 0.0403 0.0000000403 0.125943917

- 0.90
43.00 316.15 0.003163056 24.2906 0.0490 0.0000000490 0.153132803

- 0.81
44.00 317.15 0.003153082 24.2816 0.0580 0.0000000580 0.181259236

- 0.74
45.00 318.15 0.003143171 24.2702 0.0694 0.0000000694 0.216886051

- 0.66

Typical Calculation:

 m.g 0.0000000055 × 9.813 Vp = --------- = ---------------------------- = 0.017188376

A 3.14 × 10 -6 Where, Vp = Vapour pressure (Pa)  m = Mass difference (kg) g = Acceleration due to gravity (9.813 m/s 2 ) A = Area of the orifice (3.14 × 10 -6 m 2 )
Calculation of Vapour Pressure at: 25 °C Conversion of temperature from °C to (K) = °C + 273.15 = 25 + 273.15 = 298.15

Slope (b) Log 10 [Vp(Pa)] = --------------------- + Intercept (a)

Temperature (K)

-11241.63

= -------------- + 34.75 = -2.95 298.15
Value of a and b Refer Figure 1 (Page N° 18)

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 15 of 37



### Vapour Pressure of Sodium 3-Nitrobenzene Sulfonate (CAS # 127-68-4, US EPA HPV 3)
TABLE 2 Calculation of Vapour Pressure (Replication 2)

Tempera

ture (°C) Tempera

ture (K) Reciprocal of

Temperature (1/K) Mass (mg)

of Test Item Mass Difference (mg) Mass

Difference (kg)  m Vapour

Pressure (Pa) Vp Log 10 Vp (Pa) Log 10 [Vp(Pa)] at 2 5 °C

35.00 308.15 0.0 03245173 29.2884

- - - -

- 3.04 36.00 309.15 0.003234676 29.2826 0.0058 0.0000000058 0.018125924

- 1.74
37.00 310.15 0.003224246 29.2771 0.0113 0.0000000113 0.035314299

- 1.45
38.00 311.15 0.003213884 29.2701 0.0183 0.0000000183 0.057190414

- 1.24
39.00 312.15 0.003203588 29.2635 0.0249 0.0000000249 0.077816465

- 1.11
40.00 313.15 0.003193358 29.2554 0.0330 0.0000000330 0.103130255

- 0.99
41.00 314.15 0.003183193 29.2458 0.0426 0.0000000426 0.133131783

- 0.88
42.00 315.15 0.003173092 29.2347 0.0537 0. 0000000537 0.167821051

- 0.78
43.00 316.15 0.003163056 29.2228 0.0656 0.0000000656 0.205010446

- 0.69
44.00 317.15 0.003153082 29.2100 0.0784 0.0000000784 0.245012484

- 0.61
45.00 318.15 0.003143171 29.1961 0.0923 0.0000000923 0.288452197

- 0.54

Typical Calculation:

 m.g 0.0000000058 × 9.813 Vp = --------- = ---------------------------- = 0.018125924

A 3.14 × 10 -6 Where, Vp = Vapour pressure (Pa)  m = Mass difference (kg) g = Acceleration due to gravity (9.813 m/s 2 ) A = Area of the orifice (3.14 × 10 -6 m 2 )
Calculation of Vapour Pressure at: 25 °C Conversion of temperature from °C to (K) = °C + 273.15 = 25 + 273.15 = 298.15

Slope (b) Log 10 [Vp(Pa)] = --------------------- + Intercept (a)

Temperature (K)

-12258.49

= -------------- + 38.08 = -3.04 298.15
Value of a and b Refer Figure 2 (Page N° 19)

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 16 of 37



### Vapour Pressure of Sodium 3-Nitrobenzene Sulfonate (CAS # 127-68-4, US EPA HPV 3)
TABLE 3 Calculation of Vapour Pressure (Replication 3)

Tempera

ture (°C) Tempera

ture (K) R eciprocal of

Temperature (1/K) Mass (mg)

of Test Item Mass Difference (mg) Mass

Difference (kg)  m Vapour

Pressure (Pa) Vp Log 10 Vp (Pa) Log 10 [Vp(Pa)] at 2 5 °C

35.00 308.15 0.003245173 28.2148

- - - -

- 2.92 36.00 309.15 0.003234676 28.2089 0.0059 0.00000 00059 0.018438439

- 1.73
37.00 310.15 0.003224246 28.2004 0.0144 0.0000000144 0.045002293

- 1.35
38.00 311.15 0.003213884 28.1944 0.0204 0.0000000204 0.063753248

- 1.20
39.00 312.15 0.003203588 28.1874 0.0274 0.0000000274 0.085629363

- 1.07
40.00 313.1 5 0.003193358 28.1795 0.0353 0.0000000353 0.110318121

- 0.96
41.00 314.15 0.003183193 28.1687 0.0461 0.0000000461 0.144069841

- 0.84
42.00 315.15 0.003173092 28.1582 0.0566 0.0000000566 0.176884013

- 0.75
43.00 316.15 0.003163056 28.1461 0.0687 0.000000 0687 0.214698439

- 0.67
44.00 317.15 0.003153082 28.1335 0.0813 0.0000000813 0.254075446

- 0.60
45.00 318.15 0.003143171 28.1201 0.0947 0.0000000947 0.295952580

- 0.53

Typical Calculation:

 m.g 0.0000000059 × 9.813 Vp = --------- = ---------------------------- = 0.018438439

A 3.14 × 10 -6 Where, Vp = Vapour pressure (Pa)  m = Mass difference (kg) g = Acceleration due to gravity (9.813 m/s 2 ) A = Area of the orifice (3.14 × 10 -6 m 2 )
Calculation of Vapour Pressure at: 25 °C Conversion of temperature from °C to (K) = °C + 273.15 = 25 + 273.15 = 298.15

Slope (b) Log 10 [Vp(Pa)] = --------------------- + Intercept (a)

Temperature (K)

-11813.04

= -------------- + 36.70 = -2.92 298.15
Value of a and b Refer Figure 3 (Page N° 20)

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 17 of 37



### Vapour Pressure of Sodium 3-Nitrobenzene Sulfonate (CAS # 127-68-4, US EPA HPV 3)
TABLE 4 Calculation of Vapour Pressure

Replication Log 10 

### Vp (Pa) at 2 5 °C

I

- 2.95

II

- 3.04

III

- 2.9 2

Mean

- 2.9 7 Vapour Pressure (Antilog) 1.0 7 × 10 - 3

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 18 of 37



### Vapour Pressure of Sodium 3-Nitrobenzene Sulfonate (CAS # 127-68-4, US EPA HPV 3)
FIGURE 1 Reciprocal Temperature (1/K) Vs Log 10 

### [Vp(Pa)]

(Replication 1)

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### Vapour Pressure of Sodium 3-Nitrobenzene Sulfonate (CAS # 127-68-4, US EPA HPV 3)
FIGURE 2 Reciprocal Temperature (1/K) Vs Log 10 

### [Vp(Pa)]

(Replication 2)

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### Vapour Pressure of Sodium 3-Nitrobenzene Sulfonate (CAS # 127-68-4, US EPA HPV 3)
FIGURE 3 Reciprocal Temperature (1/K) Vs Log 10 

### [Vp(Pa)]

(Replication 3)

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### Vapour Pressure of Sodium 3-Nitrobenzene Sulfonate (CAS # 127-68-4, US EPA HPV 3)
APPENDIX 1 Thermographs

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APPENDIX 1 (Continued)

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APPENDIX 1 (Continued)

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### Vapour Pressure of Sodium 3-Nitrobenzene Sulfonate (CAS # 127-68-4, US EPA HPV 3)
APPENDIX 2 Signed Study Plan and Study Plan Amendments

JRF Study Number: 207-2- 11 -4305 Page 1 of 9

STUDY PLAN

VAPOUR PRESSURE OF SODIUM 3-NITROBENZENE SULFONATE

(CAS # 127- 68 -4, US EPA HPV 3)

GUIDELINES: OECD N° 104 AND U.S. EPA, OPPTS 830.7950 TEST: ASTM E 1782-08 (THERMAL ANALYSIS)

TEST RULE: 40 CFR 799.5089

SPONSOR

TIARCO CHEMICAL 1300 TIARCO DRIVE DALTON GA 30720

TECHNICAL CONTACT: JEFF VANDERLIP REGULATORY CONTACT: BENNETT KING

PERFORMING LABORATORY

JAI RESEARCH FOUNDATION DEPARTMENT OF CHEMISTRY VALVADA - 396 108 DIST. VALSAD GUJARAT INDIA

FEBRUARY - 2012

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 25 of 37 APPENDIX 2 (Continued)
JRF Study Number: 207-2- 11 -4305 Page 2 of 9

CONTENTS PAGE N° TITLE PAGE

........................................................................................................................................................... 1 CONTENTS

............................................................................................................................................................. 2 1. GENERAL INFORMATION

........................................................................................................................... 3 1.1 Study Professional

................................................................................................................................ . 3 1.2 Facility Management

.............................................................................................................................. 3 1.3 Technical Contact

.................................................................................................................................. 3 1.4 Regulatory Contact

................................................................................................................................ 3 1.5 Proposed Study Dates

............................................................................................................................ 4 1.6 Study Plan and Amendment (if any) Distribution

.................................................................................. 4 2. INTRODUCTION

............................................................................................................................................ 4 2.1 Objective

................................................................................................................................................ 4 2.2 Regulatory Guidelines

............................................................................................................................ 4 2.3 Principle

................................................................................................................................................. 4 2.4 Test Item

................................................................................................................................................ 5 3. GOOD LABORATORY PRACTICE

.............................................................................................................. 6 3.1 GLP Compliance

.................................................................................................................................... 6 3.2 Standard Operating Procedures (SOP)

................................................................................................... 6 3.3 Amendment to Study Plan

..................................................................................................................... 6 3.4 Deviation from the Study Plan

............................................................................................................... 6 3.5 Quality Assurance

.................................................................................................................................. 6 4. EXPERIMENTAL PROCEDURE

................................................................................................................... 6 4.1 Instrument

.............................................................................................................................................. 6 4.2 Methodology

.......................................................................................................................................... 7 4.2.1 Determination of Vapour Pressure [Effusion Method using TGA]

............................................. 7 4.2.2 Calculation

.................................................................................................................................. 7 5. REPORT

........................................................................................................................................................... 8 6. ARCHIVES

...................................................................................................................................................... 8 7. REFERENCES

................................................................................................................................................. 8 8. STUDY PLAN APPROVAL

............................................................................................................................ 9

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 26 of 37 APPENDIX 2 (Continued)
JRF Study Number: 207-2- 11 -4305 Page 3 of 9

1. GENERAL INFORMATION

1.1 Study Professional :

Note: List of additional study personnel will be documented in the study file and will be presented in the final report.
1.2 Facility Management : Dr. Manishi Agarwal Assistant Director, Department of Chemistry Jai Research Foundation, Off. N.H. N°. 8

Near Daman Ganga Bridge Valvada. 396 108, Dist. Valsad, Gujarat, India. Ph. : + 91

- 260 654 0242 (Office) Ph : +91 99099 00929 (Mobile) E-mail: agarwalm@jrfonline.com

1.3 Technical Contact : Jeff Vanderlip 706/277-1300 Tiarco Chemical 1300 Tiarco Drive Dalton. Georgia 30720 1.4 Regulatory Contact : Bennett King 864/457-2321 Tiarco Chemical 1300 Tiarco Drive Dalton. Georgia 30720

Name of Personnel Qualification Profession Experience Training A.H. Patel (Study Director) M.Sc. Chemist 14 year s Declared Study Director for all Physico

- chemical studies

Ashok S. Amruskar (Dy. Study Director) M.Sc. Chemist 5 years Declared Study Director for all Physico

- chemical studies

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 27 of 37 APPENDIX 2 (Continued)
JRF Study Number: 207-2- 11 -4305 Page 4 of 9

1.5 Proposed Study Dates Experiment Start : March 2012* Experiment Completion : March 2012* Draft Report Submission : November 01, 2012 Final Report Submission : December 10, 2012 Note: (*) = Experiment start and completion date depends on EPA response to the study plan
1.6 Study Plan and Amendment (if any) Distribution a. Original copy in Archive and Study Sponsor; b. Photocopy to Study Director and QAU

2. INTRODUCTION 2.1 Objective The objective of this study is to determine the vapour pressure of Sodium 3-nitrobenzene sulfonate (CAS No. 127-68-4).

2.2 Regulatory Guidelines
OECD, 2006: OECD N° 104, “Vapour Pressure”. The Organisation for Economic Cooperation and Development (OECD) guidelines for the Testing of Chemicals, adopted by the Council on March 23, 2006.

Sta ndard Test Method for Determining Vapour Pressure by Thermal Analysis, ASTM E 1782-08, approved
March 1, 2008.
U.S. EPA, 1996: OPPTS 830.7950 “Vapour Pressure” (EPA 712 -C- 96 -043). The United States Environmental Protection Agency, Product Properties Test Guidelines (August 1996).

2.3 Principle The quantity of the test item leaving a cell per unit time through an aperture of known size is determined under vacuum conditions such that return of the test item into the cell is negligible (by measuring the weight loss).

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 28 of 37 APPENDIX 2 (Continued)
JRF Study Number: 207-2- 11 -4305 Page 5 of 9

2.4 Test Item The Test Item Data Sheet has been completed by the Sponsor. The representative sample of the test item will be retained for archive purpose. Any residual test item will be disposed off at JRF after the expiry date unless otherwise instructed by the Sponsor. The details relating to the test item (as furnished by the study Sponsor) are
as follows:
Test Item Name
IUPAC Name
C.A. Name
CAS N°
Batch N° Analysed Purity Manufacture by Supplied by
Date of Manufacture Date of Expiry Appearance Sodium 3

- nitrobenzene sulfonate Benzenesulfonic acid, 3

- nitro, sodium salt (1:1) Benzenesulfonic acid, 3

- nitro, sodium salt (1:1)
127

- 68

- 4
50623
96.9%
Tiarco Chemical
Tiarco Chemical September 08, 2011 Septemb er 08, 2013 Yellow to tan granular Storage Condition (at JRF) As per the instruction received from the Sponsor on storage of the test item, the test item will be stored in its original container, as supplied by the Sponsor at 40

- 90 °F in Test Item Contr ol Office (TICO). The stability of the test item in storage will be the responsibility of the Sponsor

.

JRF Test Item Code SNB 444

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 29 of 37 APPENDIX 2 (Continued)
JRF Study Number: 207-2- 11 -4305 Page 6 of 9

3. GOOD LABORATORY PRACTICE 3.1 GLP Compliance This study will be conducted in compliance with the OECD Principles of Good Laboratory Practice (as revised in 1997), ENV/MC/CHEM(98)17, N° 1, Environment Directorate, Organisation for Economic Cooperation and Development, Paris (1998).

3.2 Standard Operating Procedures (SOP) Unless otherwise specified all procedures mentioned in the study plan are subject to detailed Standard Operating Procedures of Jai Research Foundation.

3.3 Amendment to Study Plan This study plan may be subject to amendment. Amendment to study plan, whether initiated by the Sponsor or the Study Director will be generated, authorized by the Study Director and will be sent to the Sponsor for approval.

In the event that circumstances dictate immediate action, the nature of these circumstances will communicated to the Sponsor as soon as practicable (by telephone, facsimile transmission or e-mail) and will be confirmed as soon as possible by way of formal study plan amendment.

3.4 Deviation from the Study Plan Any deviation from the study plan will be documented in study file and reported in the study report.

3.5 Quality Assurance The Quality Assurance Unit (QAU) of JRF will audit the critical phases of the study, the raw data, draft and final reports. The audit reports will be provided to the Study Director and the Management. The dates of audits and reporting of findings to the Study Director and the Management will be incorporated in the study report.

4. EXPERIMENTAL PROCEDURE
4.1 Instrument Thermogravimetric analyzer (TGA).

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 30 of 37 APPENDIX 2 (Continued)
JRF Study Number: 207-2- 11 -4305 Page 7 of 9

4.2 Methodology 4.2.1 Determination of Vapour Pressure [Effusion Method using TGA] A quantity of 10-50 mg test item will be added to the balance pan of the instrument. The balance will be allowed to stand for some time to get stabilized. After the balance is stabilized, the reading will be recorded electronically. The temperature scanning will be started between the ranges of 40 to 70 °C and will be scanned for a range of 10 °C using nitrogen as purging gas @ 50mL/min. The mass (mg) and the temperature readings (°C) will be recorded automatically into a computer file and the report will be printed. The readings of temperature and mass will be converted into Kelvin (K) and Kilogram (kg), respectively. The experiment will be performed in triplicate and the mean value will be reported.

4.2.2 Calculation The vapour pressure will be calculated at each set temperature using the following formula:

Vp =

Where, Vp = Vapour pressure (Pa)  m = Mass difference (kg) g = Acceleration due to gravity (9.813 m/s 2 ) A = Area of the orifice (3.14 x 10 -6 m 2 )
After calculating the vapour pressure (Pa) the Log 10 of the value obtained will be calculated.
A graph will be plotted of reciprocal temperature (1/K) versus Log 10 [Vp(Pa)] to obtain a straight line, intercept and slope.

To obtain the vapour pressure at a particular temperature the following formula will be used:

Log 10 [Vp(Pa)] =

After calculating the Log 10 [Vp(Pa)] at the particular temperature, the anti log of the mean of the replications will be calculated and reported.

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 31 of 37 APPENDIX 2 (Continued)
JRF Study Number: 207-2- 11 -4305 Page 8 of 9

5. REPORT Unless otherwise requested by the Sponsor, one copy of the final report will be issued along with one soft copy in PDF. The report will include the following information:

- Description of the test item including certificate of analysis - Details of the experimental procedure - Results - Personnel involved in the study - Thermographs - The signed study plan and study plan amendment (if any) - Records of deviation(s) from the study plan (if any)

6. ARCHIVES On completion of the study, all original raw data including any storage medium for electronically recorded data, documentation, the signed study plan, any study plan amendment, thermographs, the draft report, a copy of the final report and the representative sample of the test item will be retained in the GLP Archives at Jai Research Foundation for a period of ten years. At the end of this period, the Sponsor's instructions will be sought to either extend the archiving period or return the archived material to the Sponsor or for the material to be disposed off.

7. REFERENCES OECD, 1998: OECD Series on Principles of Good Laboratory Practice and Compliance Monitoring, Number 1, “OECD Principles on Good Laboratory Practice” ENV/MC/CHEM(98)17 (as revised in 1997).

OECD, 2006: OECD N° 104, “Vapour Pressure”. The Organisation for Economic Cooperation and Development (OECD) guidelines for the Testing of Chemicals, adopted by the council on March 23, 2006.

Standard Test Method for Determining Vapour Pressure by Thermal Analysis, ASTM E 1782-08, approved March 1, 2008.

U.S. EPA, 1996: OPPTS 830.7950 “Vapour Pressure” (EPA 712 -C- 96 -043). The United States Environmental Protection Agency, Product Properties Test Guidelines (August 1996).

Vohra.Y, 2006: Validation of Effusion Method for Determination of Vapour Pressure of Ethofumesate Technical, JRF Study Number 5874, Jai Research Foundation, Valvada - 396 108, Gujarat, India.

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### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 35 of 37



### Vapour Pressure of Sodium 3-Nitrobenzene Sulfonate (CAS # 127-68-4, US EPA HPV 3)
APPENDIX 3 Certificate of Analysis of Sodium 3-Nitrobenzene Sulfonate

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 36 of 37



### Vapour Pressure of Sodium 3-Nitrobenzene Sulfonate (CAS # 127-68-4, US EPA HPV 3)
APPENDIX 4 GLP Endorsement of Compliance

### JRF Study Number: 207-2- 11 -4305 (Final Report) Page 37 of 37

APPENDIX 4 (Continued)