13339 Hagers Ferry Road

Huntersville, NC 28078-7929

McGuire Nuclear Complex - MG03A2

Phone: 980-875-5245 Fax: 980-875-4349

Order Summary Report

Analytical Laboratory

Order Number: J11040139

Project Name: WWTS - Biweekly

Lab Contact: Jason C Perkins

Date: 4/26/2011

Customer Address: 3195 Pine Hall Rd

Customer Name(s): Bill Kennedy, Melonie Martin, Wayne Chapman, Tom Johnson

Mailcode: Belews Steam Station

Belews Creek, NC 28012

Phone: 980-875-5348

Report Authorized By:

(Signature)

Program Comments:

Belews Bi Monthly - 4/13

Data Flags & Calculations:

Any analytical tests or individual analytes within a test flagged with an "X" or "1" indicate a deviation from the method quality 

system or quality control requirement. All results are reported on a dry weight basis unless otherwise noted.

Data Package:

This data package includes analytical results that are applicable only to the samples described in this narrative. An estimation of 

the uncertainty of measurement for the results in the report is available upon request. This report shall not be reproduced, except 

in full, without the written consent of the Analytical Laboratory. Please contact the Analytical laboratory with any questions. The 

order of individual sections within this report is as follows:

Job Summary Report, Sample Identification, Technical Validation of Data Package, Analytical Laboratory Certificate of Analysis, 

Analytical Laboratory QC Reports, Sub-contracted Laboratory Results, Customer Specific Data Sheets, Reports & 

Documentation, Customer Database Entries, Test Case Narratives, Chain of Custody (COC)

Certification:

The Analytical Laboratory holds the following State Certifications : North Carolina (DENR) Certificate #248, South Carolina 

(DHEC) Laboratory ID # 99005. Contact the Analytical Laboratory for definitive information about the certification status of 

specific methods. 

Page 1 of 27
Sample ID's & Descriptions:

Sample ID Plant/Station

Collection 

Date and Time Collected By Sample Description

2011007553 BELEWS 13-Apr-11 12:15 PM W.B. WORKMAN FGD Purge Eff 

2011007575 BELEWS 13-Apr-11 12:20 PM W. B. WORKMAN EQ TANK EFF. 

2011007576 BELEWS 13-Apr-11 12:25 PM W. B. WORKMAN BIOREACTOR 1 INF. 

2011007577 BELEWS 13-Apr-11 12:35 PM W. B. WORKMAN BIOREACTOR 2 INF. 

2011007578 BELEWS 13-Apr-11 12:30 PM W. B. WORKMAN BIOREACTOR 2 EFF. 

2011007579 BELEWS 06-Apr-11 2:20 PM L.DAVIS Trip Blank 

2011007580 BELEWS 06-Apr-11 2:20 PM L.DAVIS FILTER BLANK 

2011007581 BELEWS 13-Apr-11 12:25 PM W. B. WORKMAN BIOREACTOR 1 INF. 

2011007582 BELEWS 13-Apr-11 12:45 PM W. B. WORKMAN HG BLANK BIOREACTOR 1 INF. 

2011007583 BELEWS 13-Apr-11 12:35 PM W. B. WORKMAN BIOREACTOR 2 INF. 

2011007584 BELEWS 13-Apr-11 12:45 PM W. B. WORKMAN Hg Blk BioReactor 2 Inf 

2011007585 BELEWS 13-Apr-11 12:30 PM W. B. WORKMAN BIOREACTOR 2 EFF. 

2011007586 BELEWS 13-Apr-11 12:45 PM W. B. WORKMAN Hg Blk BioReactor 2 Eff 

13 Total Samples

Page 2 of 27
COC and .pdf report are in agreement with sample totals 

and analyses (compliance programs and procedures).

All Results are less than the laboratory reporting limits.

All laboratory QA/QC requirements are acceptable.

The Vendor Laboratories have been qualified by the 

Analytical Laboratory

Yes No

Technical Validation Review

Checklist:

Yes No

Yes No

Report Sections Included:

Job Summary Report Sub-contracted Laboratory Results

Sample Identification Customer Specific Data Sheets, Reports, & Documentation

Technical Validation of Data Package Customer Database Entries

Analytical Laboratory Certificate of Analysis Test Case Narratives

Analytical Laboratory QC Report Chain of Custody

Reviewed By: DataBase Administrator Date: 4/26/2011

Yes

Electronic Data Deliverable (EDD) Sent Separately

Page 3 of 27
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J11040139

2011007553

Collection Date: 13-Apr-11 12:15 PM

Site: FGD Purge Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 165 ug/L EPA 245.1 5 15-Apr-11 15:04 TLINN

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 203 mg/L EPA 200.7 0.5 20-Apr-11 11:42 DJSULL1

DISSOLVED METALS BY ICP-MS

Selenium (Se) 127 ug/L EPA 200.8 10 20-Apr-11 14:16 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 145 ug/L EPA 200.8 10 20-Apr-11 11:07 KRICHAR

Chromium (Cr) 200 ug/L 10 EPA 200.8 20-Apr-11 11:07 KRICHAR

Copper (Cu) 121 ug/L 10 EPA 200.8 20-Apr-11 11:07 KRICHAR

Nickel (Ni) 181 ug/L 10 EPA 200.8 20-Apr-11 11:07 KRICHAR

Selenium (Se) 6230 ug/L 20 EPA 200.8 20-Apr-11 11:07 KRICHAR

Silver (Ag) < 10 ug/L 10 EPA 200.8 20-Apr-11 11:07 KRICHAR

Zinc (Zn) 235 ug/L 20 EPA 200.8 20-Apr-11 11:07 KRICHAR

SELENIUM SPECIATION

Vendor Parameter Complete V_AS&C

TOTAL DISSOLVED SOLIDS

TDS 18000 mg/L SM2540C 100 19-Apr-11 09:15 TJA7067

2011007575

Collection Date: 13-Apr-11 12:20 PM

Site: EQ TANK EFF. 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 114 ug/L EPA 245.1 2.5 15-Apr-11 15:06 TLINN

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 183 mg/L EPA 200.7 0.5 20-Apr-11 11:46 DJSULL1

DISSOLVED METALS BY ICP-MS

Selenium (Se) 78.3 ug/L EPA 200.8 10 20-Apr-11 14:20 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 75.7 ug/L EPA 200.8 10 20-Apr-11 10:33 KRICHAR

Chromium (Cr) 115 ug/L 10 EPA 200.8 20-Apr-11 10:33 KRICHAR

Copper (Cu) 67.0 ug/L 10 EPA 200.8 20-Apr-11 10:33 KRICHAR

Nickel (Ni) 128 ug/L 10 EPA 200.8 20-Apr-11 10:33 KRICHAR

Page 4 of 27
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J11040139

2011007575

Collection Date: 13-Apr-11 12:20 PM

Site: EQ TANK EFF. 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

TOTAL RECOVERABLE METALS BY ICP-MS

Selenium (Se) 3290 ug/L EPA 200.8 10 20-Apr-11 10:33 KRICHAR

Silver (Ag) < 10 ug/L 10 EPA 200.8 20-Apr-11 10:33 KRICHAR

Zinc (Zn) 149 ug/L 20 EPA 200.8 20-Apr-11 10:33 KRICHAR

2011007576

Collection Date: 13-Apr-11 12:25 PM

Site: BIOREACTOR 1 INF. 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 162 mg/L EPA 200.7 0.5 20-Apr-11 11:50 DJSULL1

DISSOLVED METALS BY ICP-MS

Selenium (Se) 69.6 ug/L EPA 200.8 10 20-Apr-11 14:24 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 10 ug/L EPA 200.8 10 20-Apr-11 10:28 KRICHAR

Chromium (Cr) < 10 ug/L 10 EPA 200.8 20-Apr-11 10:28 KRICHAR

Copper (Cu) < 10 ug/L 10 EPA 200.8 20-Apr-11 10:28 KRICHAR

Nickel (Ni) 20.0 ug/L 10 EPA 200.8 20-Apr-11 10:28 KRICHAR

Selenium (Se) 76.2 ug/L 10 EPA 200.8 20-Apr-11 10:28 KRICHAR

Silver (Ag) < 10 ug/L 10 EPA 200.8 20-Apr-11 10:28 KRICHAR

Zinc (Zn) < 20 ug/L 20 EPA 200.8 20-Apr-11 10:28 KRICHAR

SELENIUM SPECIATION

Vendor Parameter Complete V_AS&C

2011007577

Collection Date: 13-Apr-11 12:35 PM

Site: BIOREACTOR 2 INF. 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 157 mg/L EPA 200.7 0.5 20-Apr-11 11:54 DJSULL1

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 10 ug/L EPA 200.8 10 20-Apr-11 10:24 KRICHAR

Chromium (Cr) < 10 ug/L 10 EPA 200.8 20-Apr-11 10:24 KRICHAR

Copper (Cu) < 10 ug/L 10 EPA 200.8 20-Apr-11 10:24 KRICHAR

Nickel (Ni) < 10 ug/L 10 EPA 200.8 20-Apr-11 10:24 KRICHAR

Selenium (Se) < 10 ug/L 10 EPA 200.8 20-Apr-11 10:24 KRICHAR

Silver (Ag) < 10 ug/L 10 EPA 200.8 20-Apr-11 10:24 KRICHAR

Zinc (Zn) < 20 ug/L 20 EPA 200.8 20-Apr-11 10:24 KRICHAR

Page 5 of 27
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J11040139

2011007578

Collection Date: 13-Apr-11 12:30 PM

Site: BIOREACTOR 2 EFF. 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) < 1 ug/L EPA 245.1 1 15-Apr-11 15:08 TLINN

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 144 mg/L EPA 200.7 0.5 20-Apr-11 11:58 DJSULL1

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 5 ug/L EPA 200.8 5 20-Apr-11 10:19 KRICHAR

Chromium (Cr) < 5 ug/L 5 EPA 200.8 20-Apr-11 10:19 KRICHAR

Copper (Cu) < 5 ug/L 5 EPA 200.8 20-Apr-11 10:19 KRICHAR

Nickel (Ni) < 5 ug/L 5 EPA 200.8 20-Apr-11 10:19 KRICHAR

Selenium (Se) < 5 ug/L 5 EPA 200.8 20-Apr-11 10:19 KRICHAR

Silver (Ag) < 5 ug/L 5 EPA 200.8 20-Apr-11 10:19 KRICHAR

Zinc (Zn) < 10 ug/L 10 EPA 200.8 20-Apr-11 10:19 KRICHAR

SELENIUM SPECIATION

Vendor Parameter Complete V_AS&C

2011007579

Collection Date: 06-Apr-11 2:20 PM

Site: Trip Blank 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

TOTAL RECOVERABLE METALS BY ICP

Boron (B) < 0.05 mg/L EPA 200.7 0.05 20-Apr-11 11:18 DJSULL1

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 1 ug/L EPA 200.8 1 20-Apr-11 10:14 KRICHAR

Chromium (Cr) < 1 ug/L 1 EPA 200.8 20-Apr-11 10:14 KRICHAR

Copper (Cu) < 1 ug/L 1 EPA 200.8 20-Apr-11 10:14 KRICHAR

Nickel (Ni) < 1 ug/L 1 EPA 200.8 20-Apr-11 10:14 KRICHAR

Selenium (Se) 1.88 ug/L 1 EPA 200.8 20-Apr-11 10:14 KRICHAR

Silver (Ag) < 1 ug/L 1 EPA 200.8 20-Apr-11 10:14 KRICHAR

Zinc (Zn) < 2 ug/L 2 EPA 200.8 20-Apr-11 10:14 KRICHAR

SELENIUM SPECIATION

Vendor Parameter Complete V_AS&C

2011007580

Collection Date: 06-Apr-11 2:20 PM

Site: FILTER BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

DISSOLVED METALS BY ICP-MS

Page 6 of 27
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J11040139

2011007580

Collection Date: 06-Apr-11 2:20 PM

Site: FILTER BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

DISSOLVED METALS BY ICP-MS

Selenium (Se) < 2 ug/L EPA 200.8 2 20-Apr-11 14:29 KRICHAR

2011007581

Collection Date: 13-Apr-11 12:25 PM

Site: BIOREACTOR 1 INF. 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY 1631

Vendor Parameter Complete V_BRAND

2011007582

Collection Date: 13-Apr-11 12:45 PM

Site: HG BLANK BIOREACTOR 1 INF. 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY 1631

Vendor Parameter Complete V_BRAND

2011007583

Collection Date: 13-Apr-11 12:35 PM

Site: BIOREACTOR 2 INF. 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY 1631

Vendor Parameter Complete V_BRAND

2011007584

Collection Date: 13-Apr-11 12:45 PM

Site: Hg Blk BioReactor 2 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY 1631

Vendor Parameter Complete V_BRAND

2011007585

Collection Date: 13-Apr-11 12:30 PM

Site: BIOREACTOR 2 EFF. 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY 1631

Vendor Parameter Complete V_BRAND

Page 7 of 27
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J11040139

2011007586

Collection Date: 13-Apr-11 12:45 PM

Site: Hg Blk BioReactor 2 Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY 1631

Vendor Parameter Complete V_BRAND

Page 8 of 27
 

April 22, 2011 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

(704) 875-5245 

Project: Belews – FGD WWTS (2011, Bi-Weekly Sampling) (LIMS # J11040139) 

Dear Mr. Perkins, 

Attached is the report associated with four (4) aqueous samples submitted for selenium 

speciation analysis on April 14, 2011. The samples were received on April 15, 2011 in a 

sealed cooler at -0.5o

C. Selenium speciation analysis was performed via ion 

chromatography inductively coupled plasma dynamic reaction cell mass spectrometry 

(IC-ICP-DRC-MS). Any analytical issues associated with the analysis are addressed in 

the following report. 

If you have any questions, please feel free to contact me at your convenience. 

 Sincerely, 

 

 Russell Gerads 

 Vice President 

 Applied Speciation and Consulting, LLC 

Page 9 of 27
Applied Speciation and Consulting, LLC 

Report prepared for: 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

Project: Belews – FGD WWTS (2010, Bi-Weekly Sampling) (LIMS # J11040139) 

April 22, 2011 

1. Sample Reception 

Four (4) aqueous samples in 125mL HDPE bottles (provided by Applied Speciation 

and Consulting) were submitted for selenium speciation analysis on April 14, 2011. 

The samples were received on April 15, 2011 in a sealed container at -0.5o

C. 

The samples were received in a laminar flow clean hood void of trace metals 

contamination and ultra-violet radiation. Upon reception, the samples were 

designated discrete sample identifiers. An aliquot of each sample was filtered 

(0.45μm) and these filtrates were stored in a secure, monitored cryofreezer 

(maintained at a temperature of -80oC) until selenium speciation analysis could be 

performed via ion chromatography inductively coupled plasma dynamic reaction cell 

mass spectrometry (IC-ICP-DRC-MS). 

2. Sample Preparation 

All sample preparation is performed in laminar flow clean hoods known to be free 

from trace metals contamination. All applied water for dilutions and sample 

preservatives are monitored for contamination to account for any biases associated 

with the sample results. 

Selenium Speciation Analysis by IC-ICP-DRC-MS Prior to analysis, an aliquot of 

each sample was filtered with a syringe filter (0.45μm) and injected directly into a 

sealed autosampler vial. No further sample preparation was performed as any 

chemical alteration of the samples may shift the equilibrium of the system resulting in 

changes in speciation ratios. 

Page 10 of 27
3. Sample Analysis 

All sample analysis is precluded by a minimum of a five-point calibration curve 

spanning the entire concentration range of interest. Calibration curves are performed 

at the beginning of each analytical day. All calibration curves, associated with each 

species of interest, are standardized by linear regression resulting in a response factor. 

All sample results are instrument blank corrected to account for any operational 

biases associated with the analytical platform. 

Prior to sample analysis, all calibration curves are verified using second source 

standards which are identified as initial calibration verification standards (ICV). 

Ongoing instrument performance is identified by the analysis of continuing 

calibration verification standards (CCV) and continuing calibration blanks (CCB) at a 

minimal interval of every ten analytical runs. 

Selenium Speciation Analysis by IC-ICP-DRC-MS All samples for selenium 

speciation analysis were analyzed by ion chromatography inductively coupled plasma 

dynamic reaction cell mass spectrometry (IC-ICP-DRC-MS) on April 15, 2011. An 

aliquot of each sample is injected onto an anion exchange column and mobilized by a 

basic (pH > 7) gradient. The eluting selenium species are then introduced into a radio 

frequency (RF) plasma where energy-transfer processes cause desolvation, 

atomization, and ionization. The ions are extracted from the plasma through a 

differentially-pumped vacuum interface and travel through a pressurized chamber 

(DRC) containing a specific reactive gas which preferentially reacts with interfering 

ions of the same target mass to charge ratios (m/z). A solid-state detector detects ions 

transmitted through the mass analyzer and the resulting current is processed by a data 

handling system. 

Retention times for each eluting species are compared to known standards for species 

identification. 

4. Analytical Issues 

The overall analyses went very well and no analytical issues were encountered. All 

quality control parameters associated with these samples were within acceptance 

limits. 

The estimated method detection limits (eMDLs) for selenite, selenate, and 

selenocyanate are generated from replicate analyses of the lowest standard in the 

calibration curve. Not all selenium species are present in preparation blanks; 

therefore, eMDL calculations based on preparation blanks are artificially biased low. 

Page 11 of 27
The eMDL for methylseleninic acid and selenomethionine is calculated from the 

average eMDL of selenite, selenate, and selenocyanate. The calibration does not 

contain methylseleninic acid or selenomethionine due to impurities in these standards 

which would bias the results for other selenium species. 

If you have any questions or concerns regarding this report, please feel free to contact 

me. 

Sincerely, 

Russell Gerads 

Vice President 

Applied Speciation and Consulting, LLC 

Page 12 of 27
Sample Results

Sample ID Se(IV) Se(VI) SeCN MeSe(IV) SeMe

Unkn

Spec

FGd Purge Eff 76.1 67.4 ND (<5.1) ND (<3.8) ND (<3.8) 0 

BioReactor 1 Inf 15.2 62.4 ND (<1.3) ND (<0.95) ND (<0.95) 0 BioReactor 2 Eff ND (<0.95) ND (<0.64) ND (<1.3) ND (<0.95) ND (<0.95) 0 Metals Trip Blk ND (<0.19) ND (<0.13) ND (<0.25) ND (<0.19) ND (<0.19) 0 All results reflect the applied dilution and are reported in μg/LND = Not detected at the applied dilutionSeCN = SelenocyanateMeSe(IV) = Methylseleninic acidSeMe = SelenomethionineUnknown Se Species = Total concentration of all unknown Se species observed by IC-ICP-MSn = number of unknown Se species observed

Report Generated by: Russell Gerads

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Belews - FGD WWTS (2010, Bi-Weekly Sampling)

Contact: Jay Perkins

Date: April 22, 2011

LIMS #J11040139
Quality Control Summary - Preparation Blank Summary

Analyte (μg/L) PBW1 PBW2 PBW3 PBW4 Mean StdDev eMDL* eMDL 10x eMSe(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.019 0.19 Se(VI) 0.000 0.000 0.000 0.000 0.000 0.000 0.013 0.13 SeCN 0.000 0.000 0.000 0.000 0.000 0.000 0.025 0.25 MeSe(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.019 0.19 SeMe 0.000 0.000 0.000 0.000 0.000 0.000 0.019 0.19 eMDL = Estimated Method Detection Limit*Please see narrative regarding eMDL calculationsQuality Control Summary - Certified Reference MaterialsAnalyte (μg/L) CRM True Value Result RecoverySe(IV) ICV 9.57 9.40 98.3Se(VI) ICV 9.48 9.02 95.2SeCN ICV 8.92 8.87 99.5MeSe(IV) ICV 6.47 5.97 92.3SeMe ICV 9.32 7.23 77.6

LIMS #J11040139

Date: April 22, 2011

Report Generated by: Russell Gerads

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Belews - FGD WWTS (2010, Bi-Weekly Sampling)

Contact: Jay Perkins
Quality Control Summary - Matrix Duplicates

Analyte (μg/L) Sample ID Rep 1 Rep 2 Mean RPDSe(IV) Batch QC 216.6 212.0 214.3 2.2Se(VI) Batch QC 242.0 244.5 243.2 1.0SeCN Batch QC ND (<5.1) ND (<5.1) NC NCMeSe(IV) Batch QC 8.4 7.3 7.9 13.9SeMe Batch QC 4.7 3.9 4.3 18.4ND = Not detected at the applied dilutionNC = Value was not calculated due to one or more concentrations below the eMDL Quality Control Summary - Matrix Spike/ Matrix Spike DuplicateAnalyte (μg/L) Sample ID Spike Conc MS Result Recovery Spike Conc MSD Result Recovery RPDSe(IV) Batch QC 1112 1294 97.1 1112 1288 96.5 0.5Se(VI) Batch QC 1009 1214 96.2 1009 1208 95.6 0.5SeCN Batch QC 915.0 801.2 87.6 915.0 792.1 86.6 0.0

LIMS #J11040139

Date: April 22, 2011

Report Generated by: Russell Gerads

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Belews - FGD WWTS (2010, Bi-Weekly Sampling)

Contact: Jay Perkins
Page 16 of 27
 

3958 6th Ave NW • Seattle, WA 98107 • T: 206-632-6206 • F: 206-632-6017 • www.brooksrand.com • brl@brooksrand.com 

April 22, 2011 

Duke Energy 

ATTN: Jay Perkins 

Scientific Support-Laboratory

13339 Hagers Ferry Road 

Huntersville NC 28078 

jcperkins@duke-energy.com 

labcustomer@duke-energy.com 

RE: Project DUK-HV1101 Client Project: J11040139 

Dear Mr. Perkins, 

On April 15, 2011, Brooks Rand Labs (BRL) received three (3) flue gas desulfurization (FGD) 

waste water samples and three (3) field blanks (one from each related FGD sample site). All 

samples were logged-in for total mercury (Hg) analysis and were received, prepared, analyzed, 

and stored according to BRL SOPs and EPA methodology. 

The results were blank-corrected as described in the calculations section of the applicable 

SOP(s) and may be evaluated using adjusted reporting limits to account for sample aliquot size. 

Please refer to the Sample Results page for sample-specific detection limits and other details. 

Aside from concentration qualifiers, all data was reported without qualification and all quality 

assurance criteria were satisfied. 

Sample Hg Blk BioReactor 2 Eff (1116035-06) was identified as a field blank and produced a 

detectable Hg concentration of 0.16 ng/L. The result was less than the method reporting limit of 

0.40 ng/L and not considered a significant source of contamination. 

BRL, an accredited laboratory, certifies the reported results of all analyses for which BRL is 

NELAP accredited meet all NELAP requirements. For more details, see the Report Information

page of the report. Please feel free to contact us if you have any questions regarding this report. 

Sincerely, 

 

Tiffany Stilwater Lydia Greaves 

Project Manager Project Coordinator 

tiffany@brooksrand.com lydia@brooksrand.com 

Page 17 of 27
Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Definition of Data Qualifiers

(Effective 9/23/09)

Laboratory Accreditation

BRL is accredited by the National Environmental Laboratory Accreditation Program (NELAP) through the State of Florida 

Department of Health, Bureau of Laboratories (E87982) and is certified to perform many environmental analyses. BRL is 

also certified by many other states to perform environmental analyses. For a current list of our accreditations /certifications, 

please visit our website at <http://www.brooksrand.com/default.asp?contentID=586>. Results reported relate only to the 

samples listed in the report.

Report Information

BLK

BRL

BS

CAL

CCV

CRM

D

DUP

ICV

MSD

ND

NR

PS

REC

RPD

RSD

SCV

SOP

method blank 

Brooks Rand Labs

laboratory fortified blank

calibration standard

continuing calibration verification

certified reference material

dissolved fraction

duplicate

initial calibration verification

matrix spike duplicate

non-detect

non-reportable

post preparation spike

percent recovery

relative percent difference

relative standard deviation

secondary calibration verification

standard operating procedure

MDL

MRL

MS

method detection limit

method reporting limit

matrix spike

SRM

T

COC

standard reference material

total recoverable fraction

chain of custody record 

Common Abbreviations

These qualifiers are based on those previously utilized by Brooks Rand, Ltd., those found in the EPA SOW ILM03.0, 

Exhibit B, Section III, pg. B-18, and the USEPA Laboratory Data Validation Functional Guidelines for Evaluating Inorganic 

Analyses; USEPA; July 2002. These supersede all previous qualifiers ever employed by BRL.

B Detected by the instrument, the result is > the MDL but ≤ the MRL. Result is reported and considered an estimate.

E An estimated value due to the presence of interferences. A full explanation is presented in the narrative.

H Holding time and/or preservation requirements not met. Result is estimated.

J Estimated value. A full explanation is presented in the narrative.

J-M Duplicate precision (RPD) for associated QC sample was not within acceptance criteria. Result is estimated.

J-N Spike recovery for associated QC sample was not within acceptance criteria. Result is estimated.

M Duplicate precision (RPD) was not within acceptance criteria. Result is estimated.

N Spike recovery was not within acceptance criteria. Result is estimated.

R Rejected, unusable value. A full explanation is presented in the narrative.

U Result is ≤ the MDL or client requested reporting limit (CRRL). Result reported as the MDL or CRRL.

X Result is not BLK-corrected and is within 10x the absolute value of the highest detectable BLK in the batch. 

Result is estimated.

Field Quality Control Samples

Please be notified that certain EPA methods require the collection of field quality control samples of an appropriate type 

and frequency; failure to do so is considered a deviation from some methods and for compliance purposes should only be 

done with the approval of regulatory authorities. Please see the specific EPA methods for details regarding required field 

quality control samples.

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 18 of 27
Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Information

Sample Lab ID Report Matrix Type Sampled Received

BioReactor 1 Inf 1116035-01 FGD Wastewater Sample 04/13/2011 04/15/2011

Hg Blk BioReactor 1 Inf 1116035-02 DIW Field Blank 04/13/2011 04/15/2011

BioReactor 2 Inf 1116035-03 FGD Wastewater Sample 04/13/2011 04/15/2011

Hg Blk BioReactor 2 Inf 1116035-04 DIW Field Blank 04/13/2011 04/15/2011

BioReactor 2 Eff 1116035-05 FGD Wastewater QC Sample 04/13/2011 04/15/2011

Hg Blk BioReactor 2 Eff 1116035-06 DIW Field Blank 04/13/2011 04/15/2011

Batch Summary

Analyte Lab Matrix Method Prepared Analyzed Batch Sequence

Hg Water EPA 1631 04/18/2011 04/21/2011 B110554 1100263

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 19 of 27
Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Results

Sample Analyte Report Matrix Fraction Result Qualifier MDL MRL Unit Batch Sequence

BioReactor 1 Inf

1116035-01 Hg FGD Wastewater T 80.8 1.52 4.04 ng/L B110554 1100263

BioReactor 2 Eff

1116035-05 Hg FGD Wastewater T 24.9 1.52 4.04 ng/L B110554 1100263

BioReactor 2 Inf

1116035-03 Hg FGD Wastewater T 171 1.52 4.04 ng/L B110554 1100263

Hg Blk BioReactor 1 Inf

1116035-02 Hg DIW T 0.15 U 0.15 0.40 ng/L B110554 1100263

Hg Blk BioReactor 2 Eff

1116035-06 Hg DIW T 0.16 B 0.15 0.41 ng/L B110554 1100263

Hg Blk BioReactor 2 Inf

1116035-04 Hg DIW T 0.15 U 0.15 0.41 ng/L B110554 1100263

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 20 of 27
Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Accuracy & Precision Summary

Analyte Native Spike Result Units REC & Limits RPD & Limits

Batch: B110554

Method: EPA 1631

Lab Matrix: Water

Sample

B110554-SRM1 Certified Reference Material (1116002, NIST 1641d 1000x dilution)

Hg 15.68 15.92 ng/L 102% 85-115

B110554-MS1 Matrix Spike (1116035-05)

Hg 24.88 121.2 160.4 ng/L 112% 71-125

B110554-MSD1 Matrix Spike Duplicate (1116035-05)

Hg 24.88 121.2 156.6 ng/L 109% 71-125 242%

Method Blanks & Reporting Limits

Batch: B110554

Method: EPA 1631

Matrix: Water

Analyte: Hg

Sample Result Units

B110554-BLK1 0.12 ng/L

B110554-BLK2 0.19 ng/L

B110554-BLK3 0.07 ng/L

B110554-BLK4 0.05 ng/L

Average: 0.11 Standard Deviation: 0.06 MDL: 0.15

Limit: 0.50 Limit: 0.10 MRL: 0.41

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 21 of 27
Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Instrument Calibration

Method: EPA 1631

Sequence: 1100263

Instrument: THG-05

Date: 04/21/2011

Total Mercury and Mercury Speciation by CVAFS

Analyte: Hg

Lab ID True Value Result Units REC & Limits

1100263-IBL1 6.35 pg of Hg

1100263-IBL2 6.90 pg of Hg

1100263-IBL3 6.34 pg of Hg

1100263-IBL4 6.83 pg of Hg

1100263-CAL1 25.00 24.78 pg of Hg 99%

1100263-CAL2 100.0 98.98 pg of Hg 99%

1100263-CAL3 500.0 474.7 pg of Hg 95%

1100263-CAL4 2500 2590 pg of Hg 104%

1100263-CAL5 10000 10390 pg of Hg 104%

1100263-ICV1 1568 1592 pg of Hg 102% 85-115

1100263-CCB1 14.1 pg of Hg

1100263-CCV1 500.0 499.3 pg of Hg 100% 77-123

1100263-CCV2 500.0 484.7 pg of Hg 97% 77-123

1100263-CCV3 500.0 513.9 pg of Hg 103% 77-123

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 22 of 27
Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Containers

Lab ID: 1116035-01 Report Matrix: FGD Wastewater

Sample: BioReactor 1 Inf Sample Type: Sample Received: 04/15/2011

Collected: 04/13/2011

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71281400

20

Bottle FLPE Hg-T 250 mL None N/A Cooler

Lab ID: 1116035-02 Report Matrix: DIW

Sample: Hg Blk BioReactor 1 Inf Sample Type: Field Blank Received: 04/15/2011

Collected: 04/13/2011

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71281400

20

Bottle FLPE Hg-T 250 mL None N/A Cooler

Lab ID: 1116035-03 Report Matrix: FGD Wastewater

Sample: BioReactor 2 Inf Sample Type: Sample Received: 04/15/2011

Collected: 04/13/2011

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71281400

20

Bottle FLPE Hg-T 250 mL None N/A Cooler

Lab ID: 1116035-04 Report Matrix: DIW

Sample: Hg Blk BioReactor 2 Inf Sample Type: Field Blank Received: 04/15/2011

Collected: 04/13/2011

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71281400

20

Bottle FLPE Hg-T 250 mL None N/A Cooler

Lab ID: 1116035-05 Report Matrix: FGD Wastewater

Sample: BioReactor 2 Eff Sample Type: QC Sample Received: 04/15/2011

Collected: 04/13/2011

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71281400

20

Bottle FLPE Hg-T 250 mL None N/A Cooler

Lab ID: 1116035-06 Report Matrix: DIW

Sample: Hg Blk BioReactor 2 Eff Sample Type: Field Blank Received: 04/15/2011

Collected: 04/13/2011

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71281400

20

Bottle FLPE Hg-T 250 mL None N/A Cooler

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 23 of 27
Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Shipping Containers

Cooler

Tracking No: 4726 7965 9580 via FedEx

Temperature: 1.9 °C

Coolant Type: Ice

Description: Cooler

Damaged in transit? No

Returned to client? No

Custody seals present? No

Custody seals intact? No

COC present? Yes

Received: April 15, 2011 8:40

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

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