### UNITED STATES ENVIRONMENTAL PROTECTION AGENCY WASHINGTON , D .C

. 2 0460



## OCT 1 5 2015 OFFICE OF CHEMICA L SAFETY ANO POLLUTION PREVENTION

Step h en L. D obson, Ph.D. U ni vers ity of Ke ntu cky, D epartmen t of Entomo logy S-225 Agricu ltura l Scie nc e Center North Lex in g ton, KY 40546-0091 S ubj ect: Ex perimental Use Pennit Issua nce for 

### Wolba chia pipientis, wA lbB Stra in in Male 

### Aedes aegypti WB l Strain Mosquitoes ( Including Egg, La rva l, Pupal , and Adult Life Stages) in Ca lifornia 

### Expe rim e n tal Use Permit No.: 88877-EUP-2 

### OPP Deci s ion No .: 500458 

### E ffecti ve Dates: Immediatel y until December 3 1. 2016 (pesticide applications and

associated activities , e.g., collectio n of field data) 

### Quantity Auth o riz ed : 2.400,000 male 

### Aedes aegypti WBI Strain mosquitoes , weighing



### 5.672 ounces 

### (160.8 grams) and containing 

### 5.672 x 10 · 5 ounce 

### (l.608 x 10· 3 gran1) of act i ve ingredient 

### Ac r es In vo lve d : 840 acres Dear Dr. D obson: On th e bas is of th e in fo rm ation furn ished b y you, the s ubj ec t Expe rim e ntal Use Pennit (EUP) under sec ti o n 5 of th e Federa l In secticide , Fun g ic ide, and Rodent ic i de Act (FIFRA) is hereb y issued for the pesticidal act ive in gredient 

### Wolbachia pipientis, wA l bB St rain in ma l e 

### Aedes aegypti WB 1 Stra in mosquitoes. The EUP, which is authorized only in the state of Ca li fornia, w ill eva luate the active ingredient's effecti veness in suppress in g and elim inatin g 

### Aedes aegypti m osqu itoe s at particular s ites in Fresno Cou nty. Shipment and/or use under this permit are subject to the provisions of 40 CFR Part 172. Prior to s hipm e nt and/or u se of this material. you mu s t consu lt w ith the pesticide regulatory officials of the s tate s in which yo u will conduct your experimental program and obta in a state permit or license if s uch is required. I ssuance of this Federal permit doe s not negate the need for pem1ission from individual s tate s. Prior to initiating this experimental program in any s tate. yo u are required to n otify the lead agency of the s tate s in which you will conduct your experimental program of the spec i fic testing program (w hen. where. how much, etc.). Failure to obtain that perm iss ion may result in revocation or modification of the EUP. You mu s t provide notice to the s tate pesticide regulatory agency at lea s t 72 h ours prior to the app li cation of this experi m e nt a l pestic ide product. You must a lso provide a copy of this au thor ization letter and the

### Page 2of3 EPA EUP No. 88877 -EUP -2 OPP Decision No. 500458 stampe d , accepted EUP labeling to all cooperators, participants, and users prior to the initial pesticide application made in accordance wi th this E U P. The U.S. Environmental Protecti on Agency (EPA) w ill notify the re levant EPA Region of the approval of thi s EUP by sending copies of this EUP authori zation lette r and th e stamped, accepted EUP labelfn g to · it. Based on the experime ntal program submitted , this product may be shipped for use und e r this EUP to the state of Ca li forn ia for the next exper imental period , effective immediately until December 31. 2016. Within 840 acres of Fresno County. you may release. monitor , and collect data on 2,400 .000 male 

### Aedes aegypti WB I Stra in mosquitoes that weigh 5.672 ounces (oz) ( 160.8 grams (g)) and con tain 5.672 x 10· 5 

### oz (1

. 608 x I 0 · 3 

### g) of 

### Wolbachia pipientis , wAlbB Stra in. You will immediately notify th e EPA of any findings from the experim ental u ses that have a bearing on safety (i.e

.. the EPA require s reporting of any adverse effects from the use of o r exposure to pesticide s)

. You will also keep records of production, distribut ion . and per formance, and make the records available on request to any authorized officer or employee of the EPA. Prior to registrat ion o r amendmen t under F IFRA sec tion 3, a ll data requirement s mu s t be sati sfied (40 CFR Part 1 58, Subpart V). The labeling sub mitted in connection w ith the application fo r th e EU P is acceptable. and a stamped. accepted copy is enclosed for your records. Thi s labeling mu st be u sed for a ll s hipments under this EU P and must b e in possession of the user at th e time of pesticide app li catio n. You mu st provide a fina l rep o rt at the conclusion of the experiment. Thi s final report sha ll include a ll of the items set fort h in 40 C FR 

### § I 72.8(b)(2). In addition, you must perform the fo ll owi ng testing and monitoring and include the results of this testing and monitoring in the final report: (1) Sample the mosquito population used to produce male wAlbB Strain 

### Aedes aegypti mo s quitoes for poss i b le infection wit h pathogenic v iru ses s uch as de n g ue ; Eastern equ in e e ncep h a liti s; West N il e virus; other arbov iru ses; and lymp hatic , subc utaneous, and serious cavity fi l ariasis. (2) Monitor the release of wA lbB Stra in-infected 

### Aedes aegypri fe male m osq uito es at the release s ites. If polyme rase chai n reaction ( P C R) is used to monitor for the wA lbB Strai n , de sc rib e how the se wA lbB Strain-infected female mosqui toes w ill be d istinguished from the w ild 

### Aedes aegypti fema le mo squ itoes th at have mated w ith wAlbB S tra in-in fected 

### Aedes aegypti male mosquitoes. In other words, describe how you ensure that the wi ld type female mosquitoes do not appear wAlbB Strain p ositive (us in g PCR) after fertilization wit h wAlbB Stra in-inf ected male mosquitoes. (3) Samp le re leased mo sq ui toes to confirm the rate of female mosquitoes released. (4) Monitor population density for an imp ac t of male mosquito releases throu g h trappin g of

### Aedes aegypti mosquitoes and additional mosquitoes that co-occur at the sites and that are collected in traps.Page 3o f 3 EPA EU P No

. 88877- EU P- 2 O PP D ec is io n No

. 500458 (5) Assess r e le ase m a le mos qui to quality b y h old in g a s ub set in a cage a nd monitorin g s urvival/ft tne ss . (6) Mark a s ub set of re lease male m osqui toes wi th dus t and then monitor for th e ir recapture ( m arkr e lea se -recapture). (This addresses questions of the release to indi ge n o u s male mosquito ratio and re lease mal e m osqui to s u rviva l. ) (7) Collect eggs fro m th e fie l d to exami ne fo r a reduction in egg hatch

. ( 8) M o nito r e n v ironm e n ta l co ndition s (temperatur e. wind s pe e d , etc.) fr o m a local Nat ional Oceanic and Atmospheric Ad mini s tration s tati o n. If yo u ha ve a n y question s reg arding this permit. please con tact J eann in e Kau sc h by p h o ne at ( 703 ) 3 47- 8920 or v ia email at kau sc h.j eannine @ epa . gov.

E nc l osu re

# s~Zi**~)·** lf' Rob e rt M c a ll y. Director Bi opes ti c id es and Pollution

Preve nti o n Di v is ion (75 11 P) Offi ce of Pestic ide P rograms

cc: Patti TenBrook. EPJ\ Reg io n 9 ( California)