# STUDY NO. 00

- 6130

SPONSOR STUDY NO. 211

- DIPE -S

GASOLINE DIPE VAPOR CONDENSATE:

A 13

- WEEK WHOLE

- BODY INHALATION TOXICITY STUDY IN RATS

WITH NEUROTOXICITY A SSESSMENTS AND 4

- WEEK IN VIVO

GENOTOXICITY AND IMMUNOTOXICITY ASSESSME NTS

Final Report

Volume II of IV

Submitted to: American Petroleum Institute (API)

1220 L Street, Northwest

Washington, DC 20005

- 4070

Attn: Thomas M. Gray, M.S., D.A.B.T.

Date: 31 May 2013

Page 381 of 1446

# Huntingdon Life Sciences 00-6130 Page 382

211- DIPE -S Final Report

TABLE OF CONTENTS

Volume II

COVER PAGE .................................................................................................................381

TABLE OF CONTENTS .................................................................................................382

APPENDICES

E. Individual Body Weight Change from Baseline ........................................................383

F. Individual Feed Consumption Values ........................................................................399

G. Individual Motor Activity Values ..............................................................................419

H. Individual Functional Observational Battery Evaluations .........................................435

I. Individual Hematology Values ..................................................................................472

J. Individual Coagulation Values ...................................................................................513

K. Individual Clinical Chemistry Values ........................................................................530

L. Individual Organ Weights and Brain Measurements .................................................591

M. Individual Animal Gross and Microscopic Observations ..........................................63026-SEP-200310:02HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)MALESANIMAL#MEANS.D.N10511052105310541055105610571058105910601061106210631064106510661067106810691070GROUP10MG/M3WEEKOFSTUDY0-14241393932433348213845393545333627262239367.5200-276777274547763834877757473736554615053666710.620APPENDIXEGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTS0-310195979986101821106897105107101999388907779979410.8200-41171231271229011711011183108125115137127114132110949811711414.020INDIVIDUALBODYWEIGHTCHANGEFROMBASELINE(GRAMS)0-513313214013911114512813910514314914013515413614513111410513413313.8200-614314115814912415814216111915616815716516216016914513312315214915.0200-716416217517014416816119613919419317718818818519817615314818517318.0200-817417419217615618216820416020220619321320520321219016016118718618.8200-919118521219316819218522017721822521422722121423020717617320520219.9200-1019719823320418020719323119119523323424223221823921519619322021219.3200-1121820624620919422221124720621824924925325323425022721120422922719.5200-1222721126020920323622125421022325426026525725026223922521624823720.820Page 38326-SEP-200310:02HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)MALESGROUP22000MG/M3WEEKOFSTUDYANIMAL#0-10-2APPENDIXEGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALBODYWEIGHTCHANGEFROMBASELINE(GRAMS)0-30-40-50-60-70-80-90-100-110-12-----------------------------------------------------------------------------------------------------------------------------------2051377194115125151164 185 199205217181205226538196126134154 16217117518519320533762911151271471721851992132262312054306192118128140160172186 1932012102055276383113133161179187201216229 23820564574105138149168 189199213219 228237205736669612213815216918320020521522420583869109135157 178205221 242248266 2692059336994124139158180204222 22324525820604375102128 144165 184201209223238248MEAN356695120136 155175190204212225229S.D.6.36.68.812.210.813.3 15.2 16.919.319.622.6 27.7N101010101010101010101010Page 38426-SEP-200310:02HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)MALESGROUP310000MG/M3WEEKOFSTUDYANIMAL#0-10-230512452305230703053175530543364305533733056255130574781305839613059296030602952MEAN3162S.D.8.410.2N1010APPENDIXEGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALBODYWEIGHTCHANGEFROMBASELINE(GRAMS)0-30-40-50-60-70-80-90-100-110-127197113134162176187207215221102133152 175198218 225238242 25672107124 138 17417819020521321689119133154183195215222240249100117136160181206217 2292412496790100124144161171 184197200104130153176201219 244 269 2892998110712213815817518820020521381100126147161 177188204218223731021201381571701811892022098411012814817218720121522623313.914.316.517.618.720.723.425.627.6 29.810101010101010101010Page 38526-SEP-200310:02HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)MALESANIMAL#MEANS.D.N40514052405340544055405640574058405940604061406240634064406540664067406840694070GROUP420000MG/M3WEEKOFSTUDY0-12528263226334235192933283736292740471941327.5200-251605353576468714960585575595861708740706110.420APPENDIXEGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTS0-375857780778810097699385781118382879211662998713.4200-41041091021039310510911087112109691391041091241191498111810818.020INDIVIDUALBODYWEIGHTCHANGEFROMBASELINE(GRAMS)0-5124129129122114127134124110139117771551181231381331759013612620.6200-61411481431431231431571441291521389517713913615515119710715814421.9200-716917017016914117118017715417716110920617315918717323112818216925.6200-818417618218515615819318617119417811822118617319818124814619518126.7200-920118820119417518221019119022019113323720519121419927615722119929.1200-1021619522020918519421620520123920714724721419622120729516623621130.6200-1123520723621718620322622020025022015226323221723921731617425522334.7200-1223621924321919121023122320926323316126723222524622933318526323135.720Page 38626-SEP-2003lO:OSHuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)APPENDIXEGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSMALESGROUP1ANIMAL#lOSl10S210S310S4lOSS10S610S710S810S910601061106210631064106S10661067106810691070MEANS.D.N0MG/M3WEEKOFSTUDY0-130-140-lS2432S626421922023226726627320221S231"209226 2362472SS27S218 226 23226628S281227223239224224233TSTSTSTSTSTSTSTSTSTS2322402SO22.723.720.9101010TS=TerminalSacrificeINDIVIDUALBODYWEIGHTCHANGEFROMBASELINE(GRAMS)0-160-17272 274233240279 2812392412482S928829723723930S3092Sl2492392442S92632S.O2S.S1010Page 38726-SEP-200310:19HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)MALESGROUP420000MG/M3WEEKOFSTUDYANIMAL#0-130-1440512362534052219234405324626140542252404055184203405621422140572312464058232 2384059214 22240602692704061TS4062TS4063TS4064TS4065TS4066TS4067TS4068TS4069TS4070TSMEAN227 239S.D.22.420.1N1010TS=TerminalSacrificeAPPENDIXEGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALBODYWEIGHTCHANGEFROMBASELINE(GRAMS)0-150-160-17267 269276244255259271 281296266279282220221229237 244 24725627026925026727423725126329630530525426427021.923.022.3101010Page 38826-SEP-200310:20HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)FEMALESGROUP1ANIMAL#MEANS.D.N155115521553155415551556155715581559156015611562156315641565156615671568156915700MG/M3WEEKOFSTUDY0-1914218919121113151618277222414191320156.1200-21525162826272129243625274232284227562034299.520APPENDIXEGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTS0-321352837334025402137314048384937426732483710.6200-431473758soso344438534449534350so55714556489.120INDIVIDUALBODYWEIGHTCHANGEFROMBASELINE(GRAMS)0-5315840675559384943565455634569566590so595513.1200-638563670646142514456605463515163709144695713.1200-7406647837472555847586063657075687811263886716.1200-8457449947768546355727070676273689711768957217.6200-95479539878726469607981688261817510711771927716.6200-1059886398928472756187847484727482103132721018317.1200-11649266104968867786010278808578937110412881958616.8200-12618967112978974827088104798277897611512779978817.120Page 38926-SEP-200310:20HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)FEMALESGROUP2ANIMAL#2000MG/M3WEEKOFSTUDYAPPENDIXEGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALBODYWEIGHTCHANGEFROMBASELINE(GRAMS)0-10-2 0-3 0-4 0-5 0-60-70-80-90-100-110-12-----------------------------------------------------------------------------------------------------------------------------------2551173141495757717176889185255218203850535266717172757925531312274240334956 57 576371255427374050606771798895981002555233450606477 77 79869196 93255620334552 58637083921019410525572349626463718786901059811025584102036 3535495453 5561682559213644 45586665687274758225602440486265748694 92109 104118MEAN1930415155606974 78858591S.D.6.512.5 11.88.910.015.212.9 12.714.219.215.5 16.7N101010101010101010101010Page 39026-SEP-200310:20HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)FEMALESGROUP310000MG/M3WEEKOFSTUDYANIMAL#0-10-2APPENDIXEGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALBODYWEIGHTCHANGEFROMBASELINE(GRAMS)0-30-40-50-60-70-80-90-100-110-12-----------------------------------------------------------------------------------------------------------------------------------355123414464707384 88971051031063552274755 59727588921061121161133553021223538435349607060583554102535585762728599105116121355582337525555707083819096355629526671859298104121 12913112735571725 274250526070798280893558183652 596877857692100104101355921292951667774931091029411135602544 4768 798097 99107102112108MEAN18344156646978839599101103S.D.9.211.013.911.3 14.215.014.816.517.517.020.419.3N101010101010101010101010Page 39126-SEP-200310:20HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)FEMALESGROUP4ANIMAL#45514552455345544555455645574558455945604561456245634564456545664567456845694570MEANS.D.N20000MG/M3WEEKOFSTUDY0-113-273617241612151381225211716111410136.5200-22410331621263732252923152439322930282527267.120APPENDIXEGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTS0-33119361829325642293732273652303632382537349.2200-43227452939464147385347353958355245403837417.920INDIVIDUALBODYWEIGHTCHANGEFROMBASELINE(GRAMS)0-54037493345555760466254385570395153534852509.2200-645335737485859695268594353714759586253565410.1200-751416742586256745565615341855765666647705911.5200-854446951617076705680705458845267727662596410.7200-962457061678078816385785462885973888468757111.8200-1066517662708672917191845674836076878071827411.4200-1172528569728370917185876169966781868368837711.0200-1265598177729381916995916469926684899180787911.320Page 39226-SEP-200310:20HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)FEMALESGROUP10MG/M3WEEKOFSTUDYANIMAL#0-130-14155165711552919415536367155411410915559396155682 871557786315587785155964 66156079841561TS1562TS1563TS1564TS1565TS1566TS1567TS1568TS1569TS1570TSMEAN8182S.D.15.8 15.2N1010TS=TerminalSacrificeAPPENDIXEGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALBODYWEIGHTCHANGEFROMBASELINE(GRAMS)0-150-160-177577719897 95776978112121 12710510110092 91 9264677284 848169728083857486878715.7 16.8 17.1101010Page 39326-SEP-200310:50HuntingdonLifeSciences00-6130NNeurotoxicitySub-GroupMALESGROUP4ANIMAL#40714072407340744075MEANS.D.N20000MG/M3WEEKOFSTUDYAPPENDIXEGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALBODYWEIGHTCHANGEFROMBASELINE(GRAMS)0-10-20-30-40-50-60-70-80-90-100-110-123156821031271381651611821922022184887119141173201218 244 259 2812963123771100121140159 178 189210228240253427697118139 131136130137139147 133387397121142 156 177 198210223 238247397399121144157 175 184200212225 2336.311.013.013.4 17.427.129.642.544.652.4 55.265.45 5 5 5 55 5 5 5 5 5 50-1321530825412925023166.25Page 39426-SEP-200313:49HuntingdonLifeSciences00-6130nNeurotoxicitySub-GroupFEMALESGROUP1ANIMAL#0MG/M3WEEKOFSTUDYAPPENDIXEGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALBODYWEIGHTCHANGEFROMBASELINE(GRAMS)0-10-20-30-40-50-60-70-80-90-100-ll0-120-13-----------------------------------------------------------------------------------------------------------------------------------15711630354763636869778079908315722342515975768410299109ll4 ll810315732349557282 89102106ll312312314014015742446556083859496105110ll5 ll9121157521213442 464358 5558 56646561MEAN2137465669718186909699106102S.D.3.1ll.610.9ll.815.5 18.618.522.322.627.226.029.031.0N55 55 555 5 5 5 5 55Page 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39626-SEP-200313:49HuntingdonLifeSciences00-6130nNeurotoxicitySub-GroupFEMALESGROUP3ANIMAL#10000MG/M3WEEKOFSTUDYAPPENDIXEGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALBODYWEIGHTCHANGEFROMBASELINE(GRAMS)0-10-20-30-40-50-60-70-80-90-100-110-120-13-----------------------------------------------------------------------------------------------------------------------------------3561221333946526372708197 949135621017153136 353244 48 49 48645535631630304449546265646867 7573356415284150597082768795101102100356523405071838894109117118 118136140MEAN13273447556066737782869492S.D.7.98.913.1 15.1 18.120.223.623.526.3 26.5 28.127.632.0N5 5 5 5 5 5 5 5 5 5 5 55Page 39726-SEP-200313:49HuntingdonLifeSciences00-6130nNeurotoxicitySub-GroupFEMALESGROUP420000MG/M3WEEKOFSTUDYANIMAL#0-10-2APPENDIXEGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALBODYWEIGHTCHANGEFROMBASELINE(GRAMS)0-30-40-50-60-70-80-90-100-110-120-13-----------------------------------------------------------------------------------------------------------------------------------45712641 4757758396 94111120127 127 1254572284256556467 747377829310299457332437475465 68706882848966457414 132734403446515249626685457523424951707682839710499100105MEAN193243 49616573 748187939796S.D.10.413.011.49.114.119.018.615.923.526.823.6 22.322.0N5 5 55 5555 5 5 5 5 5Page 39826-SEP-200313:52HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)MALESANIMAL#MEANS.D.N10511052105310541055105610571058105910601061106210631064106510661067106810691070GROUP10MG/M3WEEKOFSTUDYAPPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)01 234567891011100978694101100969996103951029610010410595918999974.9207977737885837978778778818384908478747281804.6207070666875717170708069737173756874696572713.4206661626371646465637263726672686770706165663.7206458596163586254586160636667666767645863623.7206355545764585657636960625363665961595559604.2206057565663595959586659605759665962635861602.9205955555259535559566254575659585760565459572.52052515250565351545358so555555565153514855532.52052soso475553525354554954515451465453so54522.52052so50495452515351424856so55494551525053513.22051so4645555349soso56465246544842515147soso3.6201247484644535349484850454947so474348514750482.720Page 39926-SEP-200313:52HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)MALESGROUP22000MG/M3WEEKOFSTUDYANIMAL#0120518974205293762053947820541028320559171205610180205792772058100802059967820609878MEAN9677S.D.4.73.2N1010APPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)23456 7 8 9101169 6658 58635856 54 52507268 6361625957 5453517065 615858 54504847 4572676458 56 55 52 52514869 656156 555148 49 484575 71706059 565148 464565626056 5449 474744 4470676659595856 53504974716760626057 545252726463595752504948 4771676359 5955525149 482.73.03.61.83.03.5 3.82.83.02.8101010101010101010101239504348434543495245463.910Page 40026-SEP-200313:52HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)MALESGROUP3ANIMAL#10000MG/M3WEEKOFSTUDYAPPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)01 234567 8 9101112-----------------------------------------------------------------------------------------------------------------------------------3051958871696859656054 54 5349483052916367626054 535049 464644453053937072676858636154 525249 473054978070666156 58555352505048305597788069 6460666058565248 4730569879 7570SF64736361575351483057105806769 67 6459575355 5649 46305896836764 6555575553so5145 4630599879736660606054534848 47 44306095777267 64606258 555252 51soMEAN967871 676459615754 525148 47S.D.3.76.94.12.53.13.35.73.93.23.42.82.31.6N1010101091010101010101010SF=SpilledFeederPage 40126-SEP-200313:52HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)MALESANIMAL#40514052405340544055405640574058405940604061406240634064406540664067406840694070MEANS.D.NGROUP4EF=EmptyFeeder20000MG/M3WEEKOFSTUDYAPPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)01 23456789101193971009893931071031021029295938891951021099491975.7207175787972718878788476757471767579807177764.4206569717271667778737070706962686873696568703.8206163646965627375707267656659656668646365664.0205960646360566368666161536158636466606161613.5205555616058556863656354495552576059585456584.7205355565858546966636060635557585658575556584.220535255605554EF60595855575654575654556350563.0195250535654476757585454545152545149495150534.2205249535354486754575551545051515149495252534.1205047515252506053535250554949515049485053512.8204145304449495952504948524946494848464849485.520124444464849495649504747524645484746454747482.720Page 40226-SEP-200314:00HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)MALESGROUP10MG/M3WEEKOFSTUDYANIMAL#13 14105147 49105248511053434410544044lOSSso531056so54105745 45105846 49105946 44106049511061TS TS1062TS TS1063TS TS1064TS TS1065TS TS1066TSTS1067TS TS1068TS TS1069TS TS1070TS TSMEAN4648S.D.3.03.9N1010TS=TerminalSacrificeAPPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTS1548484345515446474751483.210INDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)1647464246515246494648472.910174649404151so45474348463.510Page 40326-SEP-2003.14:00HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)MALESGROUP420000MG/M3WEEKOFSTUDYANIMAL#1314405142 4740524245405344 48405446514055425340564550405748574058465040594753406045 474061TSTS4062TS TS4063TS TS4064TS TS4065TSTS4066TSTS4067TSTS4068TSTS4069TS TS4070TS TSMEAN45soS.D.2.23.7N1010TS=TerminalSacrificeAPPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTS1546454754535259555249514.310INDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)16464447so47so58545147494.2101743424749494755514946483.710Page 40426-SEP-200314:04HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)FEMALESGROUP1ANIMAL#MEANS.D.N155115521553155415551556155715581559156015611562156315641565156615671568156915700MG/M3WEEKOFSTUDY0101959499999594949793901001069010888100104CF96975.3191808082798284768176777778847992838182CF83813.619CF=ContaminatedFeederAPPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTS2717681767972757671767574737985788077CF79773.719INDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)36873797273807373686871726873767281707079734.12046775757172707168706969676969797477646573713.9205607172676966687265647164626672696660CF67673.8196616571656973696761597162606664657563CF68664.51976170716865696863606064605964736175626370654.82086267726665656365636166615959626170585764633.82096361746065686467605765606060686268566058634.620106663696164706364585961605760605860586264623.620115858676356585859556451595456655060525958584.520125457645858596260594863565556556057515657573.820Page 40526-SEP-200314:04HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)FEMALESGROUP2ANIMAL#2000MG/M3WEEKOFSTUDYAPPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)01 23456789101112-----------------------------------------------------------------------------------------------------------------------------------2551938374717062576461606359 582552948079 787066 6465636262585725539985 85 84SF70727370687068652554938475 697065636163626156582555908170706659625657 57575556255694787475 686367647066 68626425571119586776964 68 646165675866255811378888175657270646467 646225599478 746863 62635456 51 535458256099866872726568646365 675964MEAN988377 75696466646362 635961S.D.8.05.16.95.33.52.94.55.84.65.05.44.53.8N1010101091010101010101010SF=SpilledFeederPage 40626-SEP-200314:04HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)APPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)FEMALESGROUP310000MG/M3WEEKOFSTUDYANIMAL#01 2345678 91011355190757472726567656057 575535529686817176726768716968623553957277 687167 67 68676661553554101757070725961 6463 66 63593555CF6976 7664 62 6766605961583556978377737267 69 64 65 666358355794CF68 67675962606359 59613558948276 727162656261655956355910477 66 66 666064606159 52553560100838077 76707269 6566 6662MEAN977875 71 7164 666564636158S.D.4.45.85.03.53.94.53.13.33.54.1 4.52.8N9 910101010101010101010CF=ContaminatedFeeder1253646060586258595560593.110Page 40726-SEP-200314:04HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)APPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSFEMALESGROUP4ANIMAL#MEANS.D.N4551455245534554455545564557455845594560456145624563456445654566456745684569457020000MG/M3WEEKOFSTUDY01 28999939210191989097111949899106926410310310195969.4207472696875787978749068SF9076808877917878787.1197276757075737775698471887982777575787374764.720SF=SpilledFeederINDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)37172656676737572688071797379717.573756969724.22046670646371726071698269SF7075697369716770704.71956468676569666870657662667377686866685869684.32066666646569676567637661726474696769656166673.82076769626066676363607364705567676767655965654.2208646862596365676362776365SF66656264636160643.81996361575763655962606958646266626263605865623.120106178575859635960597056666769616263595662625.420115864535759585956566455625568626058595758593.720125565535557626057596655606269635660595660604.120Page 40826-SEP-200314:06HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)FEMALESGROUP10MG/M3WEEKOFSTUDYANIMAL#1314155154811552575915536066155454 5515555961155657641557555915586167155952 58156049551561TSTS1562TSTS1563TSTS1564TSTS1565TSTS1566TSTS1567TSTS1568TSTS1569TSTS1570TSTSMEAN5662S.D.3.87.5N1010APPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTS1561576455555856575955583.010INDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)16SF556155545957605753572.791756596555566263605952594.110SF=SpilledFeederTS=TerminalSacrificePage 40926-SEP-200314:06HuntingdonLifeSciences00-6130ToxicitySub-Group(MainandRecovery)FEMALESGROUP4ANIMAL#45514552455345544555455645574558455945604561456245634564456545664567456845694570MEANS.D.N20000MG/M3WEEKOFSTUDYAPPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)1314 15161755626010054 5653 57586455 59556056 5657606264TSTSTSTSTSTSTSTSTSTSTSTSTSTSTSTSTSTSTSTS56642.813.01010607055566662SF565871626.1959855553666270585973649.81059625755605955586670604.710SF=SpilledFeederTS=TerminalSacrificePage 41026-SEP-200314:10HuntingdonLifeSciences00-6130NNeurotoxicitySub-GroupMALESGROUP10MG/M3WEEKOFSTUDYANIMAL#01APPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)234 5 6 7 8 9101112 13-----------------------------------------------------------------------------------------------------------------------------------10711048675 716664586153 52soso49461072968274716666 6260545652so514310731008174 665763 6459 5552 524951481074968173706465626055 565152so4810759477 67636158 555349soso4746 45MEAN9882736863 63605953 535149 4946S.D.3.8 3.2 3.43.33.5 3.2 3.53.32.4 2.71.11.72.02.2N5 5 555 5 5 5 5 5 555 5Page 41126-SEP-200314:10HuntingdonLifeSciences00-6130NNeurotoxicitySub-GroupMALESGROUP2ANIMAL#2000MG/M3WEEKOFSTUDYAPPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)01 2345678910111213-----------------------------------------------------------------------------------------------------------------------------------206196777265646458 53 535351484844206296817365616155 5351soso47384220639280SF67 6263585651494947 44442064937875706461595753 555351so4620651058875 69 61606055so5348514846MEAN978174676262585552 52so49 46 44S.D.5.24.41.72.21.3 1.5 1.6 1.71.32.21.82.14.81.6N5 54 5 5 5 5 55 5 5 5 5 5SF=SpHledFeederPage 41226-SEP-200314:10HuntingdonLifeSciences00-6130NNeurotoxicitySub-GroupMALESGROUP3ANIMAL#10000MG/M3WEEKOFSTUDYAPPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)01 2345678 910111213-----------------------------------------------------------------------------------------------------------------------------------3061967569 64635755 515050474846 4330629369697062635957515350514745306396817670636457 5651 525149 47 443064100807370666257 57 54 555049 474230659483757065 61 6159 54 5353514845MEAN9678 7369646258 565252505047 44S.D.2.75.73.32.61.72.5 2.13.01.6 1.82.01.30.61.2N5555 5 5 5 5 5 5 5 5 5 5Page 41326-SEP-200314:10HuntingdonLifeSciences00-6130NNeurotoxicitySub-GroupMALESGROUP4ANIMAL#20000MG/M3WEEKOFSTUDYAPPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)01 2345 678 910111213-----------------------------------------------------------------------------------------------------------------------------------40719376676259605857 5353so49 48 45407295766762605857535049 49 46434140731048273 6962615957so51525149 45407493796869 64 656456 595262484846407593837769 66675655535551so4946MEAN9679716662 62595553525349 4845S.D.4.53.24.43.72.93.5 3.11.63.42.05.11.72.5 2.2N5 5 55 5 5 5 5 5 55 5 5 5Page 41426-SEP-200314:10HuntingdonLifeSciences00-6130NNeurotoxicitySub-GroupFEMALESGROUP10MG/M3WEEKOFSTUDYANIMAL#01157110279157299871573101821574948215759587MEAN9884S.D.3.4 3.5N5 5APPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)2 345678 910117674 686962 6254 57 57578275 69726460616362588072 68 63 656356575556776968716362586057 598179676666 675962 6263797468 68 6463586059582.54.00.83.41.62.82.83.03.03.05 555 5 55 55 512135547575258475851605758 511.84.35 5Page 41526-SEP-200314:10HuntingdonLifeSciences00-6130NNeurotoxicitySub-GroupFEMALESGROUP22000MG/M3WEEKOFSTUDYANIMAL#01APPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)2 3 4 5 6 78 910111213-----------------------------------------------------------------------------------------------------------------------------------256184CF104716875676761706361645625629910080776469716559646659 59512563102917476 73706264 62596561625425648979787065656361575960665748256511088827968 69 68685452616058soMEAN9790847568 69 6665596163616052S.D.10.68.411.74.03.7 3.63.52.83.46.72.53.03.2 3.2N5 4 5 5 5 55 5 5 5 5 5 5 5CF=ContaminatedFeederPage 41626-SEP-200314:10HuntingdonLifeSciences00-6130NNeuro'toxicitySub-GroupFEMALESGROUP310000MG/M3WEEKOFSTUDYANIMAL#01356188723562102793563908135649276356510687MEAN9679S.D.7.75.8N5 5SF=Spi1ledFeederAPPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)23456 7891011787065 67SF6259596258747471686364636261 6275737163 666459605959686665 62 646258 59 59 55SF73706768635857 555374716865 6563595959 574.13.53.22.62.31.12.01.82.53.345 5 5 4 555 5 512135553625260535453554857523.72.25 5Page 41726-SEP-200314:10HuntingdonLifeSciences00-6130NNeurotoxicitySub-GroupFEMALESGROUP4ANIMAL#45714572457345744575MEANS.D.N20000MG/M3WEEKOFSTUDYAPPENDIXFGASOLINEDIPEVAPORCONDENSATE:A13-WEEKWHOLE-BODYINHALATIONTOXICITYSTUDYINRATSWITHNEUROTOXICITYASSESSMENTSAND4-WEEKINVIVOGENOTOXICITYANDIMMUNOTOXICITYASSESSMENTSINDIVIDUALFEEDCONSUMPTIONVALUES(GRAMS/KG/DAY)01 234567891011867869 6868 686166596058 58102897875707069 686265 65669379 77 7775 68716563596662106888580706971 716463646511888 87817477 76 7169 6965601018479767170696863 6364 6212.25.57.05.32.93.55.62.43.54.23.63.55 55 55 55 55 555121357516657625958 55635961563.83.05 5Page 418

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# Pretest Appendix G

a 

# Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group I

- 0 mg/m 3



# 1071 197 204 98 132 127 162 156 110 127 74 5 0

1072 49 131 133 65 63 45 48 10 0 0 0 0

1073 207 186 178 141 113 108 99 52 105 83 1 79

1074 20 75 208 147 134 108 29 60 0 0 0 0

1075 223 133 129 99 146 79 33 164 141 24 16 0

1076 173 150 231 202 44 85 51 5 0 0 2 1

1077 1 150 176 83 116 86 25 0 0 3 0 0

1078 27 167 132 179 151 152 99 132 78 45 0 0

1079 116 133 136 102 64 100 132 74 72 133 146 119

1080 149 107 72 129 136 130 155 87 56 35 40 31

Mean 116.2 143.6 149.3 127.9 109.4 105.5 82.7 69.4 57.9 39.7 21.0 23.0

SD 85.30 37.27 48.78 42.34 38.36 35.21 52.13 55.51 55.69 44.88 45.69 42.26

N 10 10 10 10 10 10 10 10 10 10 10 10

Group II

- 2000 mg/m 3



# 2061 174 141 95 21 8 14 7 0 2 1 0 2

2062 146 132 84 119 134 112 59 71 127 31 86 71

2063 256 257 110 113 107 65 59 7 0 1 1 0

2064 137 149 77 25 9 149 97 23 3 0 2 2

2065 175 147 133 216 143 121 49 40 40 0 0 2

2066 207 172 174 120 12 1 195 175 103 117 16 0 0

2067 11 225 196 147 149 150 57 31 8 0 0 0

2068 72 126 152 155 131 78 173 135 132 86 64 14

2069 19 224 169 160 146 109 68 107 82 17 0 0

2070 1 149 177 142 130 162 126 110 76 8 0 2

Mean 119.8 172.2 136.7 121.8 107. 8 115.5 87.0 62.7 58.7 16.0 15.3 9.3

SD 89.14 46.06 42.97 59.72 53.76 52.85 55.21 48.59 54.73 26.68 31.90 22.08

N 10 10 10 10 10 10 10 10 10 10 10 10

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# Pretest Appendix G

a 

# Number of beam breaks per interval Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group III

– 10000 mg/m 3



# 3061 147 188 56 111 120 80 0 3 0 0 2 0

3062 49 161 92 146 100 124 31 66 11 2 0 0

3063 127 98 106 119 112 130 20 8 0 9 0 2

3064 137 81 103 73 49 0 0 1 0 0 0 0

3065 22 6 14 52 129 14 6 0 0 0 0 0

3066 193 198 133 141 123 4 12 0 1 2 1 0

3067 209 209 193 202 189 1 33 112 65 21 5 0 0

3068 232 169 132 164 134 123 152 112 123 30 0 2

3069 157 249 115 94 33 74 153 138 20 55 42 25

3070 94 42 128 89 129 48 82 43 0 0 0 3

Mean 136.7 140.1 107.2 119.1 111.8 73.0 56.8 43.6 17.6 10.3 4.5 3.2

SD 67.30 79.16 47 .95 45.12 44.13 53.95 62.41 50.64 38.00 18.19 13.19 7.74

N 10 10 10 10 10 10 10 10 10 10 10 10

Group IV

– 20000 mg/m 3



# 4071 19 170 201 81 84 7 13 94 186 142 36 6

4072 36 34 81 116 91 108 108 73 47 0 0 0

4073 11 190 60 4 0 0 0 0 0 6 0 0

4074 136 141 166 182 167 87 13 1 0 0 0 6

4075 20 83 126 125 28 103 112 5 0 0 0 0

4076 161 72 59 112 113 125 24 14 0 0 0 0

4077 138 140 90 123 101 76 66 103 12 0 0 1

4078 228 117 183 130 117 82 30 155 146 70 22 0

4079 225 243 109 187 158 1 13 122 35 1 0 0 0

4080 235 236 193 156 167 176 28 76 3 0 1 2

Mean 120.9 142.6 126.8 121.6 102.6 87.7 51.6 55.6 39.5 21.8 5.9 1.5

SD 92.68 68.96 55.19 52.49 56.03 52.54 46.45 52.82 68.86 47.55 12.62 2.46

N 10 10 10 10 10 10 10 10 10 10 10 10

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# Pretest Appendix G

a 

# Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group I

- 0 mg/m 3



# 1571 143 119 67 111 7 0 1 0 0 0 1 0

1572 127 128 25 0 0 84 0 2 0 0 0 3

1573 120 106 99 33 19 0 0 0 0 0 0 0

1574 164 137 139 131 74 145 23 0 0 0 0 0

1575 84 95 97 88 63 5 1 3 4 4 9 0

1576 27 167 132 179 151 152 99 132 78 45 0 0

1577 122 100 101 35 55 66 88 97 59 10 0 0

1578 116 113 89 103 85 100 103 70 69 151 44 88

1579 162 80 141 117 126 88 37 0 0 0 0 0

1580 126 81 108 99 84 67 84 68 49 128 121 48

Mean 119.1 112.6 99.8 89.6 66.4 70.7 43.6 37.2 25.9 33.8 17.5 13.9

SD 39.82 26.68 35.18 53.12 49.16 55.59 44.85 50.04 33.39 57.64 38.87 30.05

N 10 10 10 10 10 10 10 10 10 10 10 10

Group II

– 2000 mg/m 3



# 2561 149 154 13 2 7 0 0 4 71 67 83 1 3

2562 186 142 120 123 124 179 140 139 132 145 57 34

2563 174 156 79 19 109 3 0 18 51 135 89 42

2564 150 121 1 0 0 100 155 132 96 71 88 64

2565 122 79 39 88 40 63 9 0 0 0 0 0

2566 124 126 8 72 167 47 21 18 0 0 0 1

2567 92 93 92 9 7 68 80 60 47 31 14 6 0

2568 102 54 48 0 0 0 41 130 68 43 0 3

2569 165 159 119 90 112 130 156 113 42 47 178 87

2570 137 113 123 67 80 120 111 5 0 0 0 0

Mean 140.1 119.7 76.1 56.3 70.0 72.2 69.7 67.3 48.7 53.8 41.9 23.4

SD 30.58 35.45 49. 21 45.67 58.99 61.28 64.57 56.81 43.92 54.25 60.63 31.85

N 10 10 10 10 10 10 10 10 10 10 10 10

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# Pretest Appendix G

a 

# Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group III

– 10000 mg/m 3



# 3561 164 208 80 27 44 77 0 0 0 0 0 0

3562 121 130 78 0 0 0 0 0 0 0 0 34

3563 128 90 6 0 4 1 0 0 4 0 0 1

3564 185 77 34 13 0 0 0 0 0 0 0 0

3565 162 153 116 70 179 167 168 58 8 0 0 0

3566 70 105 110 62 73 124 94 79 100 34 16 0

3567 156 73 70 70 0 0 6 70 137 126 103 81

3568 123 108 110 85 32 101 48 94 100 79 44 10

3569 119 92 132 82 74 65 82 7 8 0 0 0

3570 57 133 62 17 4 76 143 119 134 0 0 0

Mean 128.5 116.9 79.8 42.6 41.0 61.1 54.1 42.7 49.1 23.9 16.3 12.6

SD 40.88 40.98 39.19 34.37 56.79 59.69 64.38 46.33 60.33 44.17 33.57 26.31

N 10 10 10 10 10 10 10 10 10 10 10 10

Group IV

– 20000 mg/m 3



# 4571 195 142 41 130 79 109 3 0 1 0 1 1

4572 76 128 90 56 26 64 25 0 0 0 0 2

4573 77 36 1 3 0 99 98 41 46 175 27 0

4574 181 100 49 87 102 24 3 0 0 0 0 0

4575 99 141 114 110 127 11 7 158 125 137 125 88 39

4576 160 114 96 98 91 78 60 70 58 90 61 80

4577 186 149 138 63 94 29 65 68 0 29 94 10

4578 86 126 98 69 92 58 74 46 48 66 69 71

4579 53 122 113 122 101 85 82 116 81 0 0 33

4580 116 126 89 64 128 22 84 24 134 163 106 22

Mean 122.9 118.4 82.9 80.2 84.0 68.5 65.2 49.0 50.5 64.8 44.6 25.8

SD 52.82 32.27 40.84 37.58 40.90 35.15 46.88 45.91 53.30 70.02 43.64 29.78

N 10 10 10 10 10 10 10 10 10 10 10 10

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# 4 th 

# week of exposure Appendix G

a 

# Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group I

- 0 mg/m 3



# 1071 180 145 129 187 241 179 105 77 133 166 81 5

1072 183 212 163 194 37 1 0 0 0 0 0 0

1073 263 258 108 204 152 115 174 171 11 46 113 5

1074 176 121 123 54 105 55 7 4 0 0 0 0

1075 186 157 216 141 110 93 69 46 58 98 65 0

1076 179 144 61 151 93 63 73 22 1 0 1 0

1077 216 107 135 100 147 42 37 117 31 45 0 2

1078 197 179 105 125 33 6 1 4 0 0 0 0

1079 214 209 148 128 55 87 20 2 1 1 15 0

1080 142 142 172 114 126 143 96 123 135 13 1 1

Mean 193.6 167.4 136.0 139.8 109.9 78.4 58.2 56.6 37.0 36.9 27.6 1.3

SD 32.12 46.75 42.37 46.41 62.49 57.02 56.33 61.99 54.44 55.63 42.38 2.06

N 10 10 10 10 10 10 10 10 10 10 10 10

Group II

– 2000 mg/m 3



# 2061 139 122 148 102 22 42 10 0 20 4 1 0

2062 166 126 1 42 143 119 113 156 101 182 139 182 141

2063 248 190 58 6 2 33 32 10 1 0 11 0

2064 185 131 101 81 143 53 11 32 0 1 2 0

2065 241 221 151 216 184 167 110 15 0 2 10 8

2066 202 147 58 1 33 0 0 2 0 0 0 0

2067 121 41 117 0 0 0 12 2 10 2 7 5

2068 182 129 110 99 232 151 68 0 14 0 0 0

2069 175 178 107 89 12 0 21 3 0 6 0 3

2070 201 164 121 127 107 161 42 23 1 11 0 2

Mean 186.0 144.9 111.3 86.4 85.4 72.0 46.2 18.8 22.8 16.5 21.3 15.9

SD 39.89 48.78 33.00 69.37 83.35 69.28 50.89 30.84 56.40 43.18 56.63 44.04

N 10 10 10 10 10 10 10 10 10 10 10 10

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# 4 th 

# week of exposure Appendix G

a 

# Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group III

– 10000 mg/m 3



# 3061 138 146 142 46 22 0 1 0 1 0 2 1

3062 176 181 167 78 16 17 206 116 87 22 13 26

3063 320 238 137 158 66 173 9 0 0 0 0 0

3064 109 174 134 115 90 221 48 0 1 0 0 0

3065 85 174 98 60 0 0 0 0 0 4 4 3

3066 104 162 119 150 69 38 6 5 0 0 0 0

3067 197 135 205 108 151 196 104 35 17 0 4 0

3068 302 187 154 131 129 114 27 159 22 0 1 9

3069 117 159 63 24 14 10 10 2 56 110 0 0

32 128 151 42 117 75 22 0 0 0 0 0

Mean 158.0 168.4 137.0 91.2 67.4 84.4 43.3 31.7 18.4 13.6 2.4 3.9

SD 92.75 31.25 38.53 47.70 53.60 85.90 65.18 57.68 29.99 34.56 4.06 8.27

N 10 10 10 10 10 10 10 10 10 10 10 1 0

Group IV

– 20000 mg/m 3



# 4071 45 158 63 98 60 106 58 4 11 4 0 0

4072 90 127 61 137 3 4 0 0 6 0 0 0

4073 119 185 139 52 0 0 0 0 24 0 0 0

4074 232 195 167 116 107 85 179 34 5 5 9 0

4075 124 175 49 145 4 1 0 0 0 0 0 0

4076 2 29 191 220 184 139 90 7 0 8 0 0 0

4077 196 206 128 166 185 133 82 69 111 32 49 4

4078 174 132 118 93 77 79 96 105 96 24 42 2

4079 284 190 176 70 148 121 15 2 4 6 8 0

4080 177 235 153 26 6 5 0 0 0 0 0 2

Mean 167.0 179.4 127.4 108.7 72.9 6 2.4 43.7 21.4 26.5 7.1 10.8 0.8

SD 72.81 32.98 55.86 50.59 69.40 53.99 60.11 37.09 41.30 11.42 18.69 1.40

N 10 10 10 10 10 10 10 10 10 10 10 10

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# 4 th 

# week of exposure Appendix G

a 

# Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group I

- 0 mg/m 3



# 1571 120 100 5 0 0 0 0 0 0 0 0 0

1572 131 81 49 0 0 0 0 0 0 0 0 1

1573 132 94 52 109 55 30 21 14 0 0 0 0

1574 98 119 106 109 102 84 108 29 34 51 21 4

1575 165 66 37 0 0 0 0 0 0 4 0 0

1576 55 99 82 46 102 103 102 75 53 3 0 0

1577 119 142 102 105 76 35 29 17 0 0 0 0

1578 138 133 85 106 119 103 132 118 53 5 23 0

1579 171 189 111 145 8 48 44 131 64 0 0 0

1580 129 137 88 127 95 85 2 9 3 0 0 0

Mean 125.8 116.0 71.7 74.7 55.7 48.8 43.8 39.3 20.7 6.3 4.4 0.5

SD 32.78 35.74 34.55 57.24 49.25 42.31 51.13 50.19 27 .07 15.83 9.29 1.27

N 10 10 10 10 10 10 10 10 10 10 10 10

Group II

– 2000 mg/m 3



# 2561 154 111 133 145 121 74 18 12 0 0 0 0

2562 132 102 87 138 90 110 50 23 9 0 0 0

2563 83 104 93 56 28 10 0 7 3 0 0 0

2564 115 158 83 45 90 2 11 93 73 83 34 53 11

2565 143 127 100 107 87 69 0 3 4 0 1 0

2566 139 128 167 76 18 4 0 0 0 0 0 0

2567 108 100 87 97 2 41 11 0 2 5 0 9

2568 159 138 80 22 0 0 0 0 0 0 0 0

2569 161 129 22 0 105 7 1 0 0 0 0 1

2570 145 110 102 108 28 11 0 3 0 0 0 0

Mean 133.9 120.7 95.4 79.4 56.9 53.7 17.3 12.1 10.1 3.9 5.4 2.1

SD 25.00 18.61 37.31 48.27 45.89 66.66 30.92 22.62 25.77 10.69 16.73 4.20

N 10 10 10 10 10 10 10 10 10 10 10 10

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# 4 th 

# week of exposure Appendix G

a 

# Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group III

– 1 0000 mg/m 3



# 3561 138 41 0 0 0 74 19 0 0 2 0 0

3562 182 121 77 4 0 0 0 0 0 0 5 0

3563 121 167 75 85 70 81 9 3 4 0 0 0

3564 137 78 5 14 5 0 0 0 0 0 2 0

3565 165 234 176 175 109 76 131 94 6 0 0 0

3566 155 144 123 83 22 9 0 0 0 0 0 0

3567 1 23 77 106 94 27 15 0 0 0 0 0 0

3568 120 128 81 75 0 34 0 0 0 0 0 0

3569 111 102 103 86 8 21 14 6 0 1 0 0

3570 107 107 43 5 1 16 98 61 5 0 0 26

Mean 135.9 119.9 78.9 62.1 24.2 32.6 27.1 16.4 1.5 0.3 0.7 2.6

SD 24.58 53.95 53.42 56.04 36.8 6 32.22 47.21 33.19 2.46 0.67 1.64 8.22

N 10 10 10 10 10 10 10 10 10 10 10 10

Group IV

– 20000 mg/m 3



# 4571 54 126 87 0 0 0 0 0 10 6 0 1

4572 144 75 106 44 9 0 0 0 0 104 116 82

4573 38 168 134 83 87 63 4 12 0 0 0 0

4574 133 82 95 29 49 0 0 0 0 0 0 0

4575 215 192 174 113 130 146 78 59 138 146 17 74

4576 148 142 146 16 97 134 119 15 44 0 0 0

4577 138 100 92 100 76 98 44 94 110 0 0 0

4578 139 76 84 24 10 64 106 55 70 66 34 21

4579 111 101 96 118 96 92 49 27 1 0 0 3

4580 15 3 138 120 109 73 21 146 134 108 134 69 2

Mean 127.3 120.0 113.4 63.6 62.7 61.8 54.6 39.6 48.1 45.6 23.6 18.3

SD 50.50 40.14 29.66 45.49 44.06 55.43 55.11 45.60 54.37 61.18 39.57 32.16

N 10 10 10 10 10 10 10 10 10 10 10 10

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# 8 th 

# week of exposure Appendix G

a 

# Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group I

- 0 mg/m 3



# 1071 153 104 154 61 54 68 18 2 2 13 8 23

1072 161 3 2 5 0 0 1 0 0 0 1 0

1073 210 228 178 143 134 72 53 10 0 71 174 145

1074 156 92 56 0 3 0 0 0 0 0 0 0

1075 137 78 84 11 0 0 0 0 0 0 0 17

1076 153 113 31 70 0 5 3 8 0 8 0 2

1077 178 194 111 107 87 79 156 71 9 23 22 12

1078 148 182 75 87 29 2 0 0 0 0 0 0

1079 105 136 68 106 160 29 15 33 0 0 0 0

1080 190 87 33 127 91 164 21 29 1 0 0 0

Mean 159.1 121.7 79.2 71.7 55.8 41.9 26.7 15.3 1.2 11.5 20.5 19.9

SD 28.92 65.69 55.27 51.84 59.66 54.02 48.32 23.09 2.82 22.31 54.39 44.76

N 10 10 10 10 10 10 10 10 10 10 10 10

Group II

– 2000 mg/m 3



# 2061 118 137 40 10 0 6 59 2 0 4 0 0

2062 154 134 102 40 1 0 10 0 0 30 128 124

2063 151 62 25 76 7 0 1 0 0 0 0 0

2064 219 67 23 79 113 100 20 0 7 38 12 0

2065 152 154 117 104 91 118 49 4 13 0 10 1

2066 166 168 71 0 14 0 0 2 4 6 5 0

2067 186 196 19 62 5 0 38 0 0 0 0 3

2068 181 103 103 57 19 0 2 1 6 0 7 17

2069 165 155 57 0 3 1 0 0 0 1 5 2

2070 16 23 0 2 2 5 11 3 46 30 42 60

Mean 150.8 119.9 55.7 43.0 25.5 23.0 19.0 1.2 7.6 10.9 20.9 20.7

SD 54.26 54.59 40.94 38.21 41.09 45.58 21.96 1.48 14.18 15.31 39.61 40.82

N 10 10 10 10 10 10 10 10 10 10 10 10

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# 8 th 

# week of exposure Appendix G

a 

# Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group III

– 10000 mg/m 3



# 3061 103 61 12 0 59 137 13 0 2 5 2 2

3062 135 14 63 16 56 41 30 15 4 0 0 1

3063 155 58 30 2 0 0 0 0 10 0 0 4

3064 171 71 26 2 0 0 0 2 4 1 10 0

3065 132 58 38 4 6 4 10 0 0 0 6 0

3066 222 205 183 98 84 7 1 9 0 0 0 5

3067 253 236 69 78 161 58 39 1 28 4 0 6

3068 253 236 69 78 161 58 39 1 28 4 0 6

3069 102 8 0 8 4 0 1 1 0 0 0 4

3070 144 45 83 47 27 23 0 0 0 0 0 0

Mean 167.0 99.2 57.3 3 3.3 55.8 32.8 13.3 2.9 7.6 1.4 1.8 2.8

SD 56.84 89.93 51.85 38.35 62.50 43.48 16.48 5.04 11.19 2.07 3.46 2.49

N 10 10 10 10 10 10 10 10 10 10 10 10

Group IV

– 20000 mg/m 3



# 4071 146 41 56 138 37 27 54 7 0 2 5 2

4072 200 119 2 2 41 1 0 0 0 0 9 0 12

4073 80 84 15 18 0 3 0 0 0 5 0 0

4074 109 79 102 55 93 6 1 0 0 0 2 0

4075 152 91 78 26 50 71 94 0 4 5 3 1

4076 145 154 75 50 123 150 73 176 145 34 9 42

4077 227 126 126 158 57 6 90 143 50 7 136 56

4078 75 84 105 35 69 38 1 0 12 4 6 4

4079 156 95 129 14 4 3 93 152 131 113 20 0

4080 263 205 127 33 6 72 1 0 0 0 0 0

Mean 155.3 107.8 83.5 56.8 44.0 37.6 40.7 47.8 34.2 17.9 18.1 11.7

SD 60.74 45.84 42.04 49.96 42.62 48.13 43.80 75.81 56.91 34.83 41.87 20.27

N 10 10 1 0 10 10 10 10 10 10 10 10 10

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# 8 th 

# week of exposure Appendix G

a 

# Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group I

- 0 mg/m 3



# 1571 138 100 28 71 30 35 9 4 0 0 0 0

1572 57 0 0 0 0 0 0 1 9 0 0 1

1573 159 113 128 55 20 33 0 0 0 0 0 0

1574 148 139 57 94 1 0 0 0 0 0 11 80

1575 71 95 59 81 79 64 97 32 23 2 9 5

1576 134 122 148 56 24 1 1 0 0 0 0 0

1577 149 180 129 128 85 104 86 64 79 81 40 5

1578 161 132 107 128 76 108 22 0 0 0 0 0

1579 159 132 75 22 120 19 0 40 3 0 0 1

1580 163 110 151 87 90 124 30 0 12 1 0 0

Mean 133.9 112.3 88.2 72.2 52.5 48.8 24.5 14.1 12.6 8.4 6.0 9.2

SD 38.23 46.23 52.20 41.22 42.24 48.09 36.92 22.97 24.53 25.52 12.66 24.96

N 10 10 10 10 10 10 10 10 10 10 10 10

Group II

– 2000 mg/m 3



# 2561 117 55 55 63 43 0 1 0 0 0 5 0

2562 99 91 47 0 36 0 0 0 0 0 0 0

2563 88 108 30 0 0 0 0 0 0 0 14 2

2564 138 76 64 13 0 69 42 60 10 0 0 38

2565 122 79 95 76 127 86 77 130 77 87 30 4

2566 187 99 60 2 65 9 0 0 0 0 0 0

2567 128 29 61 17 3 0 0 3 0 0 0 0

2568 111 69 77 88 29 15 1 0 0 0 0 0

2569 85 3 117 38 0 0 0 0 0 0 0 0

2570 160 126 120 2 0 0 0 0 0 0 0 0

Mean 123.5 73.5 72.6 29.9 30.3 17.9 12.1 19.3 8.7 8.7 4.9 4.4

SD 31.87 36.90 29.60 34.09 41.05 32.07 26.31 43.18 24.20 27.51 9.89 11.88

N 10 1 0 10 10 10 10 10 10 10 10 10 10

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# 8 th 

# week of exposure Appendix G

a 

# Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group III

– 10000 mg/m 3



# 3561 100 79 47 7 0 1 0 0 90 13 12 5

3562 78 65 0 1 0 0 0 0 0 46 3 0

3563 96 87 64 2 2 0 0 1 3 1 0 0

3564 81 111 6 10 11 0 0 1 2 6 0 0

3565 193 71 96 53 23 0 0 2 2 14 2 0

3566 155 56 119 1 1 0 0 0 7 0 0 0

3567 190 97 131 17 0 0 0 4 1 10 0 47

3568 134 21 0 0 0 0 0 0 0 0 0 1

3569 108 72 4 0 0 0 0 0 0 1 6 0

3570 104 13 0 0 0 0 0 5 0 0 0 0

Mean 123.9 67.2 46.7 9.1 3.7 0.1 0.0 1.3 10.5 9.1 2.3 5.3

SD 42.37 30.87 52.80 16.41 7.59 0.32 0.00 1.83 28.02 14.11 3.95 14.74

N 10 10 10 10 10 10 10 10 10 10 10 10

Group IV

– 20000 mg/m 3



# 4571 110 67 4 71 3 0 29 4 0 1 5 0

4572 97 82 68 34 35 68 0 6 0 0 5 27

4573 69 93 44 1 0 0 5 0 0 0 11 0

4574 96 46 0 0 0 0 0 0 0 0 7 0

4575 160 123 92 31 37 1 0 1 0 0 2 0

4576 198 149 123 91 41 33 22 0 0 0 0 0

4577 182 172 168 84 0 0 8 3 0 0 0 0

4578 117 132 96 1 28 109 110 107 86 59 40 8

4579 136 141 84 84 113 18 5 0 0 0 0 0

4580 198 38 74 5 1 70 18 0 0 0 0 1

Mean 136.3 104.3 75.3 40.2 25.8 29.9 19.7 12.1 8.6 6.0 7.0 3.6

SD 45.98 45.77 51.03 38.61 35.25 39.27 33.30 33.41 27.20 18.62 12.17 8.59

N 10 10 10 10 10 10 10 10 10 10 10 10

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# 13 th 

# week of exposure Appendix G

a 

# Number of beam breaks per interval

b 

# Sacrificed for humane reason.

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group I

- 0 mg/m 3



# 1071 233 150 102 17 45 26 2 0 15 40 38 0

1072 215 30 71 141 32 5 9 6 7 7 0 0

1073 220 121 124 30 46 13 8 0 5 117 94 20

1074 225 122 64 15 2 0 7 12 0 2 1 0

1075 156 132 161 80 79 38 7 107 7 0 0 3

1076 67 111 71 53 87 0 2 6 0 0 0 3

1077 154 161 107 70 96 155 28 8 156 103 64 11

1078 253 158 130 42 21 8 25 2 0 47 115 1

1079 137 148 131 77 24 40 0 0 5 0 17 54

1080 163 198 121 9 27 90 26 34 59 57 7 4

Mean 182. 3 133.1 108.2 53.4 45.9 37.5 11.4 17.5 25.4 37.3 33.6 9.6

SD 56.86 44.10 31.55 40.36 31.40 49.49 10.73 33.04 49.15 44.09 43.01 16.83

N 10 10 10 10 10 10 10 10 10 10 10 10

Group II

– 2000 mg/m 3



# 2061 237 130 26 8 7 34 0 0 0 2 0 1

2062 164 90 83 11 40 1 0 3 0 0 1 0

2063 197 167 17 6 0 1 0 0 0 0 3 5

2064 232 164 146 111 46 19 11 0 0 0 0 0

2065 197 135 142 105 56 77 64 75 118 86 4 143

2066 216 88 64 91 30 56 177 51 100 2 13 0

2067 159 102 54 8 8 14 52 98 1 4 19 9

2068 192 1 08 169 69 39 12 0 0 5 0 1 4

2069 164 95 5 0 4 0 0 0 1 1 2 3

2070 b b b b b b b b b b b b



# Mean 195.3 119.9 78.4 45.4 25.6 23.8 33.8 25.2 25.0 10.6 4.8 18.3

SD 29.17 30.59 60.78 47.55 20.99 27.01 59.14 38.91 47.86 28.32 6.67 46.85

N 9 9 9 9 9 9 9 9 9 9 9 9

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# 13 th 

# week of exposure Appendix G

a 

# Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group III

– 10000 mg/m 3



# 3061 88 37 11 0 7 0 0 9 4 53 119 5

3062 87 136 46 108 27 65 10 13 40 139 36 4

3063 229 78 46 5 1 0 0 125 0 0 0 0

3064 79 140 67 45 116 115 9 0 7 0 1 15

3065 140 25 40 2 0 0 0 0 0 0 0 0

3066 205 151 127 112 16 2 32 4 0 8 0 0

3067 241 209 150 2 0 3 4 3 0 0 0 2

3068 196 106 72 101 75 48 0 21 0 0 40 22

3069 160 167 136 7 0 34 92 1 4 0 13 1

3070 198 96 23 0 15 55 14 75 23 1 0 14

Mean 162.3 114.5 71.8 38.2 25.7 32.2 16.1 25.1 7.8 20.1 20.9 6.3

SD 61.02 57.63 49.13 49.36 39.07 38.84 28.46 41.72 13.34 44.91 37.80 7.85

N 10 10 10 10 10 10 10 10 10 10 10 10

Group IV

– 20000 mg/m 3



# 4071 219 119 59 74 0 1 5 0 15 133 17 33 0

4072 137 111 8 16 31 75 2 10 1 6 0 0

4073 150 74 116 0 6 1 1 2 2 1 2 4

4074 123 114 116 104 63 14 8 11 6 0 0 1

4075 174 70 77 118 63 11 46 0 1 0 0 0

4076 138 91 57 18 23 17 3 49 90 98 5 1

4077 172 126 100 111 23 8 21 107 41 3 8 6

4078 158 120 99 90 199 138 24 10 47 2 0 0

4079 210 75 8 11 74 5 0 0 6 0 0 0

4080 254 127 89 0 0 52 0 0 0 0 0 0

Mean 173.5 102.7 72.9 54.2 48.2 33.6 10.5 20.4 32.7 12.7 4.8 1.2

SD 41.93 22.83 39.83 49.40 59.48 43.47 15.31 33.73 46.02 30.42 10.28 2.10

N 10 10 10 10 10 10 10 10 10 10 10 10

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# 13 th 

# week of exposure Appendix G

a 

# Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group I

- 0 mg/m 3



# 1571 83 128 119 119 41 1 8 82 9 82 41 1

1572 87 14 0 0 0 0 0 3 0 2 0 1

1573 185 102 91 44 0 3 4 0 0 4 27 13

1574 159 140 99 105 111 14 9 7 35 1 2 0

1575 93 61 78 96 55 51 37 0 23 3 3 0

1576 139 104 112 48 53 0 0 4 37 91 65 25

1577 144 123 99 59 120 87 72 44 12 0 0 1

1578 157 136 38 31 0 39 9 0 1 0 3 0

1579 208 105 141 141 6 0 0 0 0 0 3 0

1580 120 122 118 8 0 31 6 0 2 0 7 0

Mean 137.5 103.5 89.5 65.1 38.6 22.6 14.5 14.0 11.9 18.3 15.1 4.1

SD 42.06 38.78 41.92 47.90 46.39 29.35 22.92 27.43 14.67 36.03 22.20 8.36

N 10 10 10 10 10 10 10 10 10 10 10 10

Group II

– 2000 mg/m 3



# 2561 120 80 89 47 3 36 0 0 9 93 37 0

2562 148 84 78 54 113 28 2 0 1 9 0 0

2563 117 92 91 5 1 0 0 13 6 5 4 0

2564 127 49 18 7 19 1 5 28 11 0 0 5

2565 164 55 88 1 1 0 0 0 67 98 124 64

2566 171 56 21 2 2 0 0 1 0 0 9 2

2567 119 58 37 14 2 0 0 0 0 0 0 0

2568 146 51 109 77 1 4 152 134 140 135 50 0

2569 196 95 0 31 1 3 80 48 0 0 0 0

2570 92 63 0 0 0 0 0 6 0 0 1 1

Mean 140.0 68.3 53.1 23.8 14.3 7.2 23.9 23.0 23.4 34.0 22.5 7.2

SD 30.94 17.61 41.96 27.13 35.13 13.28 51.45 42.11 45.77 52. 73 39.83 20.02

N 10 10 10 10 10 10 10 10 10 10 10 10

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# 13 th 

# week of exposure Appendix G

a 

# Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group III

– 10000 mg/m 3



# 3561 184 100 122 88 21 0 0 3 7 7 6 2

3562 131 66 21 0 58 3 4 69 8 90 5 0

3563 139 81 69 76 0 0 0 0 0 0 1 0

3564 146 53 3 0 0 0 0 0 0 0 1 1

3565 54 62 2 0 15 125 101 40 30 10 0 1

3566 142 97 15 104 38 97 26 0 0 0 0 0

3567 188 164 99 144 20 8 0 3 0 0 1 6

3568 101 47 1 0 0 1 1 0 5 34 133 28

3569 97 135 136 74 0 0 0 0 27 0 8 2

3570 106 69 62 0 4 0 3 0 0 0 3 0

Mean 128.8 87.4 53.0 48.6 15.6 23.4 13.5 11.5 7.7 14.1 15.8 4.0

SD 40.87 37.54 52.04 54.70 19.60 46.71 31.76 23.70 11.42 28.71 41.27 8.63

N 10 10 10 10 10 10 10 10 10 10 10 10

Group IV

– 20000 mg/m 3



# 4571 1 49 102 25 21 52 0 0 0 2 3 0 13

4572 98 92 71 21 82 0 0 0 0 0 0 0

4573 122 27 25 0 0 0 0 0 1 0 0 0

4574 146 69 4 20 4 3 1 1 2 2 1 4

4575 219 155 152 100 73 89 9 1 0 0 1 1

4576 145 117 106 29 69 31 23 23 1 0 15 8

4577 122 89 113 10 0 0 2 6 0 0 0 0

457 8 129 103 57 87 40 71 9 63 74 19 0 0

4579 189 133 133 145 69 3 0 2 153 0 0 1

4580 162 75 22 0 31 0 0 0 0 0 0 0

Mean 148.1 96.2 70.8 43.3 42.0 19.7 4.4 9.6 23.3 2.4 1.7 2.7

SD 35.19 35.55 52.40 49.52 32.00 33.43 7.47 20.05 51.06 5.93 4.69 4.45

N 10 10 10 10 10 10 10 10 10 10 10 10

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Individual Functio nal Observational

Battery Evaluations

Preface Appendix H

Key:

HOME CAGE EVALUATIONS

Posture: 1

- Sitting or standing normally

(Post) 2

- Alert; sitting or standing oriented

toward the observer

3

- Asleep; may be curled up

4

- Lying on side

5

- Flattened; limbs may be spread

6

- Crouched; sitting hunched, head

hung down

Vocalizatio ns: 1

- No vocalization present

(Vocal) 2

- Vocalization present

Palpebral Closure: 1

- Eyelids open

(Palp Close) 2

- Eyelids completely closed

3

- Eyelids slightly drooping

4

- Eyelids half closed

HANDLING EVALUATIONS

Ease of Removal: 1

- Very easy; sits quietly, accepts

(Rem) observer’s touch

2

- Easy; vocalization without resistance

3

- Moderately difficult; rears, often

follows observer’s hand

4

- Freezes; with or without vocalization

5

- Difficult; runs around cage, is hard to

pick up, attempts to bite or attack,

with or without vocalization

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Individual Functio nal Observational

Battery Evaluations

Preface Appendix H

HANDLING EVALUATIONS (cont.)

Ease of Handling: 1

- Very easy; totally limp

(Hand) 2

- Normal

3

- Moderately Difficult

4

- Freezes

5

- Difficult

Chromodacryorrhea: 1

- Not present

(Chromo) 2

- Present

Lacrimation: 1

- No lacrimation

(Lac) 2

- Some lacrimation

3

- Extreme lacrimation

Coat: 1

- Normal; well groomed

2

- Slightly soiled

3

- Moderately soiled

4

- Extremely soiled; crusty unkempt

Sa livation: 1

- Not present

(Sal) 2

- Slight salivation; around mouth

3

- Moderate salivation (facial and/or

cervical area)

4

- Extreme salivation (on ventral

surface)

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Individual Functio nal Observational

Battery Evaluations

Preface Appendix H

OPEN FIELD EVALUATIONS

Gait and Posture: 1

- Normal

(Gait) 2

- Ataxia; uncoo rdinated movement,

excessive sway, rock or lurch

3

- Body drags or is flattened; animal’s

ventral surface contacts cart surface

4

- Limbs splayed or dragging (indicate hind or fore)

5

- Hunched posture

Locomotion: 1

- Normal (animal moves easily a round

(Loco) open field)

2

- Increased movement

3

- Decreased movement

4

- None, animal does not move around

field

Arousal: 1

- Normal; alert with exploratory

movements

2

- Low; slight stupor, some head or body

movements

3

- Very low; stup or, little or no

responsiveness to the environment

4

- High; slight excitement, tense, sudden

darting or freezing

5

- Very high; hyperalert, sudden boost of

running or movement

Piloerection: 1

- Not present

(Pilo) 2

- Present

Exophthalmia: 1

- Not present

(Exo) 2

- Present

Feces: U

- Unformed stool

#

- Number of pellets indicated

Urine: X

- Polyuria

#

- Number of pools indicated

# Huntingdon Life Sciences 00

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Individual Functio nal Observational

Battery Evaluations

Preface Appendix H

REFLEX ASSESSMENTS

Visual Approach: 1

- Normal, slowly approaches, sniffs

and/or turns away

2

- No re action

3

- Freezes or pulls away slightly

4

- Jumps or turns abruptly to avoid, or attacks

and/or bites

Pain Perception: 1

- Normal; turns and bites at the site or

(Pain) walks forward, away from the stimulus

2

- No reaction

3

- Increased pain; jumps forward, with or

without vocalization, or exhibits highly

exaggerated bizarre reaction, attacks, bites

Audition Assessment: 1

- Normal; flinches and/or flicks ears

(Sound) 2

- No reaction

3

- Exaggerated; jumps, flips, bites

Pupil Respons e: 1

- Pupil constricts normally

2

- Pupil size does not change

3

- Miosis (location)

4

- Mydriasis (location)

Proprioception: 1

- Returns leg to original position

(Propri) 2

- Returns leg only partially to original

position

3

- No response, rat a llows leg to remain

pulled back

Pinna Reflex: 1

- Ear flattens against head

2

- Animal shakes head

3

- No response

Air Righting Reflex: 1

- Normal; lands on four feet

2

- Slightly uncoordinated; two

- legged or

unsteady landing

3

- Lands on side

4

- Lands on back

# Huntingdon Life Sciences 00

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Individual Functio nal Observational

Battery Evaluations

Preface Appendix H

ABNORMAL MOVEMENTS

Convulsions: 1

- Not present

(Conv) 2

- Slight

3

- Extreme

Tremors: 1

- Not present

(Trem) 2

- Slight

3

- Extreme

Fasciculation: 1

- Not present

(Fasc) 2

- Slight

3

- Extreme

GRIP STRENGTH

Forelimb and hindlimb grip stre ngth measured in grams of force

LANDING FOOT SPLAY

Measured in centimeters

BODY WEIGHTS

Measured in grams

# Huntingdon Life Sciences 00

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Males Individual Functional Observational Battery Evaluations

Pretest Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp

Number Post Vo cal Close Rem Han Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group I

– 0 mg/m 3



# 1071 1 1 1 1 2 1 1 1 1 257.0 1 1 1 1 1 0 0 1 1 1

1072 1 1 1 1 2 1 1 1 1 230.9 1 1 1 1 1 0 0 1 1 1

1073 1 1 1 1 2 1 1 1 1 256.1 1 1 1 1 1 0 0 1 1 1

1074 1 1 1 1 1 1 1 1 1 227.7 1 1 1 1 1 0 0 1 1 1

1075 1 1 1 1 1 1 1 1 1 250.0 1 1 1 1 1 0 0 1 1 1

1076 1 1 1 1 2 1 1 1 1 279.6 1 1 1 1 1 3 2 1 1 1

1077 1 1 1 1 2 1 1 1 1 271.2 1 1 1 1 1 0 1 1 1 1

1078 1 1 1 1 2 1 1 1 1 259.7 1 1 1 1 1 6 0 1 1 1

1079 1 1 1 1 2 1 1 1 1 273.1 1 1 1 1 1 0 0 1 1 1

1080 2 1 1 1 2 1 1 1 1 262.2 1 1 1 1 1 0 0 1 1 1

Group II

– 2000 mg/m 3



# 2061 1 1 1 1 2 1 1 1 1 226.8 1 1 1 1 1 0 0 1 1 1

2062 1 1 1 1 2 1 1 1 1 273.2 1 1 1 1 1 0 0 1 1 1

2063 1 1 1 1 1 1 1 1 1 257.9 1 1 1 1 1 1 0 1 1 1

2064 1 1 1 1 1 1 1 1 1 217.9 1 1 1 1 1 0 0 1 1 1

2065 1 1 1 1 1 1 1 1 1 266.4 1 1 1 1 1 0 0 1 1 1

2066 1 1 1 1 2 1 1 1 1 277.0 1 1 1 1 1 0 0 1 1 1

2067 1 1 1 1 2 1 1 1 1 233.4 1 1 1 1 1 2 1 1 1 1

2068 1 1 1 1 2 1 1 1 1 285.6 1 1 1 1 1 0 2 1 1 1

2069 1 1 1 1 2 1 1 1 1 271.4 1 1 1 1 1 0 0 1 1 1

2070 1 1 1 2 2 1 1 1 1 255.7 1 1 1 1 1 0 1 1 1 1

# Huntingdon Life Sciences 00

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Males Individual Functional Observational Battery Evaluations

Pretest Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Tri al 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group I

- 0 mg/m 3



# 1071 1 1 1 1 2 1 790 875 833 715 680 698 1 5.7 5.1 5.4

1072 1 1 1 1 2 1 880 1050 965 695 610 653 1 5.1 4.8 5.0

1073 1 1 1 1 2 1 800 1050 925 660 445 553 1 9.0 7.3 8.2

1074 1 1 1 1 2 1 830 780 805 620 610 615 1 6.5 6.8 6.7

1075 1 1 1 1 2 1 780 980 880 360 410 385 1 7.8 7.4 7.6

1076 1 1 1 1 2 1 565 750 658 560 595 578 1 4.5 4.1 4.3

1077 1 1 1 1 2 1 965 1205 1085 830 715 773 1 8.2 7.4 7.8

1078 1 1 1 1 2 1 945 995 970 490 610 550 1 5.9 5.1 5.5

1079 1 1 1 1 2 1 705 1010 858 550 675 613 1 7.1 7.2 7.2

1080 1 1 1 1 2 1 785 825 805 485 385 435 1 5.5 4.3 4.9

Group II

– 2000 mg/m 3



# 2061 1 1 1 1 2 1 850 1000 925 490 565 528 1 7.2 7.1 7.2

2062 1 1 1 1 2 1 855 840 848 560 425 493 1 5.3 5.2 5.3

2063 1 1 1 1 2 1 1005 1245 1125 505 490 498 1 9.8 7.3 8.6

2064 1 1 1 1 2 1 810 960 885 460 410 435 1 4.8 5.2 5.0

2065 1 1 1 1 2 1 880 760 820 430 395 413 1 4.6 4.2 4.4

2066 1 1 1 1 2 1 780 845 813 590 690 640 1 6.0 6.2 6.1

2067 1 1 1 1 2 1 1110 1265 1188 755 690 723 1 7.7 5.4 6.6

2068 1 1 1 1 2 1 1095 980 1038 530 840 685 1 5.9 4.8 5.4

2069 1 1 1 1 2 1 720 1100 910 730 600 665 1 6.2 4.8 5.5

2070 1 1 1 1 2 1 795 1105 950 800 920 860 1 8.1 7.8 8.0

# Huntingdon Life Sciences 00

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Males Individual Functional Observational Battery Evaluations

Pretest Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp

Number Post Vocal Close Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group III

– 10000 mg/m 3



# 3061 1 1 1 1 2 1 1 1 1 246.3 1 1 1 1 1 0 1 1 1 1

3062 1 1 1 1 2 1 1 1 1 264.4 1 1 1 1 1 0 6 1 1 1

3063 1 1 1 1 2 1 1 1 1 242.2 1 1 1 1 1 0 0 1 1 1

3064 1 1 1 1 1 1 1 1 1 254.0 1 1 1 1 1 4 4 1 1 1

3065 1 1 1 1 1 1 1 1 1 212.6 1 1 1 1 1 0 0 1 1 1

3066 1 1 1 1 2 1 1 1 1 244.8 1 1 1 1 1 0 0 1 1 1

3067 1 1 1 1 2 1 1 1 1 265.9 1 1 1 1 1 1 2 1 1 1

3068 1 1 1 1 2 1 1 1 1 265.6 1 1 1 1 1 0 0 1 1 1

3069 1 1 1 1 2 1 1 1 1 283.7 1 1 1 1 1 0 0 1 1 1

3070 1 1 1 1 2 1 1 1 1 263.3 1 1 1 1 1 1 4 1 1 1

Group IV

– 20000 mg/m 3



# 4071 1 1 1 1 2 1 1 1 1 243.5 1 1 1 1 1 0 1 1 1 1

4072 1 1 1 1 2 1 1 1 1 226.8 1 1 1 1 1 0 0 1 1 1

4073 1 1 1 1 1 1 1 1 1 271.4 1 1 1 1 1 3 2 1 1 1

4074 1 1 1 1 1 1 1 1 1 255.9 1 1 1 1 1 0 2 1 1 1

4075 1 1 1 1 1 1 1 1 1 227.2 1 1 1 1 1 0 0 1 1 1

4076 1 1 1 1 2 1 1 1 1 272.5 1 1 1 1 1 0 0 1 1 1

4077 1 1 1 1 2 1 1 1 1 259.4 1 1 1 1 1 4 2 1 1 1

4078 1 1 1 2 2 1 1 1 1 299.4 1 1 1 1 1 2 1 1 1 1

4079 1 1 1 2 2 1 1 1 1 280.6 1 1 1 1 1 0 0 1 1 1

4080 1 1 1 1 2 1 1 1 1 271.7 1 1 1 1 1 1 0 1 1 1

# Huntingdon Life Sciences 00

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- S Final Report

Males Individual Functional Observational Battery Evaluations

Pretest Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group III

– 10000 mg/m 3



# 3061 1 1 1 1 2 1 535 1065 800 340 625 483 1 5.6 5.1 5.4

3062 1 1 1 1 2 1 955 970 963 590 625 608 1 6.0 4.3 5.2

3063 1 1 1 1 2 1 860 960 910 510 415 463 1 6.3 5.5 5.9

3064 1 1 1 1 2 1 900 1010 955 445 355 400 1 8.3 7.3 7.8

3065 1 1 1 1 2 1 735 1020 878 600 435 518 1 9.9 6.4 8.2

3066 1 1 1 1 2 1 920 875 898 475 720 598 1 7.7 6.7 7.2

3067 1 1 1 1 2 1 850 950 900 665 750 708 1 7.3 5.6 6.5

3068 1 1 1 1 2 1 625 990 808 760 625 693 1 5.9 5.5 5.7

3069 1 1 1 1 2 1 935 1000 968 595 450 523 1 6.6 5.2 5.9

3070 1 1 1 1 2 1 650 755 703 430 550 490 1 5.2 6.1 5.7

Group IV

– 20000 mg/m 3



# 4071 1 1 1 1 2 1 870 955 913 485 455 470 1 8.3 8.4 8.4

4072 1 1 1 1 2 1 955 885 920 715 610 663 1 7.7 8.5 8.1

4073 1 1 1 1 2 1 865 940 903 235 640 438 1 6.5 7.2 6.9

4074 1 1 1 1 2 1 1005 1020 1013 875 660 768 1 6.6 6.6 6.6

4075 1 1 1 1 2 1 595 950 773 495 430 463 1 5.2 5.6 5.4

4076 1 1 1 1 2 1 1145 1140 1143 610 660 635 1 6.0 5.7 5.9

4077 1 1 1 1 2 1 960 1040 1000 670 650 660 1 7.0 5.6 6.3

4078 1 1 1 1 2 1 855 1050 953 785 445 615 1 6.4 7.0 6.7

4079 1 1 1 1 2 1 875 1030 953 820 885 853 1 8.3 8.1 8.2

4080 1 1 1 1 2 1 1005 1205 1105 490 635 563 1 7.1 6.5 6.8

# Huntingdon Life Sciences 00

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Females Individual Functional Observational Battery Evaluations

Pretest Appendix H

Home Cage Handling Open Field

E valuations Evaluations Evaluations

Animal Palp

Number Post Vocal Close Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group I

- 0 mg/m 3



# 1571 1 1 1 1 2 1 1 1 1 200.3 1 1 1 1 1 0 0 1 1 1

1572 1 1 1 1 2 1 1 1 1 189.9 1 1 1 1 1 0 0 1 1 1

1573 1 1 1 1 1 1 1 1 1 212.0 1 1 1 1 1 0 0 1 1 1

1574 1 1 1 1 1 1 1 1 1 199.2 1 1 1 1 1 0 0 1 1 1

1575 1 1 1 1 1 1 1 1 1 186.6 1 1 1 1 1 0 2 1 1 1

1576 1 1 1 1 2 1 1 1 1 182.1 1 1 1 1 1 0 0 1 1 1

1577 1 1 1 1 2 1 1 1 1 187.0 1 1 1 1 1 0 0 1 1 1

1578 1 1 1 1 2 1 1 1 1 207.6 1 1 1 1 1 0 0 1 1 1

1579 2 1 1 1 2 1 1 1 1 207.5 1 1 1 1 1 1 1 1 1 1

1580 1 1 1 1 2 1 1 1 1 197.9 1 1 1 1 1 0 0 1 1 1

Group II

– 2000 mg/m 3



# 2561 2 1 1 1 2 1 1 1 1 191.6 1 1 1 1 1 0 0 1 1 1

2562 1 1 1 1 2 1 1 1 1 202.8 1 1 1 1 1 0 0 1 1 1

2563 1 1 1 1 2 1 1 1 1 195.7 1 1 1 1 1 0 0 1 1 1

2564 1 1 1 1 1 1 1 1 1 198.1 1 1 1 1 1 0 0 1 1 1

2565 1 1 1 1 1 1 1 1 1 182.5 1 1 1 1 1 0 0 1 1 1

2566 1 1 1 1 2 1 1 1 1 182.2 1 1 1 1 1 0 0 1 1 1

2567 1 1 1 1 2 1 1 1 1 185.6 1 1 1 1 1 0 0 1 1 1

2568 1 1 1 1 2 1 1 1 1 202.4 1 1 1 1 1 0 0 1 1 1

2 569 1 1 1 1 2 1 1 1 1 201.8 1 1 1 1 1 0 0 1 1 1

2570 2 1 1 1 2 1 1 1 1 203.1 1 1 1 1 1 0 0 1 1 1

# Huntingdon Life Sciences 00

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Females Individual Func tional Observational Battery Evaluations

Pretest Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number A pproach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group I

- 0 mg/m 3



# 1571 1 1 1 1 2 1 1145 1030 1088 830 620 725 1 4.8 5.3 5.1

1572 1 1 1 1 2 1 695 795 745 755 670 713 1 4.8 5.3 5.1

1573 1 1 1 1 2 1 860 775 818 475 765 620 1 3.8 3.6 3.7

1574 1 1 1 1 2 1 900 1045 973 680 770 725 1 5.7 5.1 5.4

1575 1 1 1 1 2 1 900 900 900 620 650 635 1 6.8 5.2 6.0

1576 1 1 1 1 2 1 665 815 740 575 620 598 1 5.3 4.9 5.1

1577 1 1 1 1 2 1 765 910 838 615 550 583 1 4.8 5.3 5.1

1578 1 1 1 1 2 1 910 805 858 615 375 495 1 4.2 3.7 4.0

1579 1 1 1 1 2 1 970 990 980 685 695 690 1 6.8 4.1 5.5

1580 1 1 1 1 2 1 865 950 908 855 805 830 1 5.3 3.9 4.6

Grou p II

– 2000 mg/m 3



# 2561 1 1 1 1 2 1 855 710 783 645 430 538 1 5.6 5.3 5.5

2562 1 1 1 1 2 1 475 870 673 625 635 630 1 4.3 5.7 5.0

2563 1 1 1 1 2 1 1070 880 975 805 610 708 1 6.2 5.3 5.8

2564 1 1 1 1 2 1 695 955 825 775 365 570 1 4.6 3.5 4.1

2565 1 1 1 1 2 1 885 1010 948 480 695 588 1 6.0 6.0 6.0

2566 1 1 1 1 2 1 1150 1145 1148 800 505 653 1 6.0 5.7 5.9

2567 1 1 1 1 2 1 980 1075 1028 610 630 620 1 4.5 4.2 4.4

2568 1 1 1 1 2 1 775 1005 890 480 435 458 1 5.0 4.9 5.0

2569 1 1 1 1 2 1 1040 995 1018 620 455 538 1 6.2 5.1 5.7

2570 1 1 1 1 2 1 850 985 918 735 595 665 1 5.6 4.8 5.2

# Huntingdon Life Sciences 00

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- S Final Report

Females Individual Func tional Observational Battery Evaluations

Pretest Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp

Number Post Vocal Close Rem Hand Chro mo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Tre Conv

(grams)

Group III

– 10000 mg/m 3



# 3561 1 1 1 1 2 1 1 1 1 190.8 1 1 1 1 1 0 0 1 1 1

3562 1 1 1 1 2 1 1 1 1 175.2 1 1 1 1 1 0 0 1 1 1

3563 1 1 1 1 1 1 1 1 1 196.0 1 1 1 1 1 0 2 1 1 1

3564 1 1 1 1 1 1 1 1 1 209.8 1 1 1 1 1 0 0 1 1 1

3565 1 1 1 1 1 1 1 1 1 209.4 1 1 1 1 1 0 0 1 1 1

3566 1 1 1 1 2 1 1 1 1 179.8 1 1 1 1 1 0 1 1 1 1

3567 2 1 1 1 2 1 1 1 1 187.2 1 1 1 1 1 0 0 1 1 1

3568 1 1 1 1 2 1 1 1 1 216.6 1 1 1 1 1 0 3 1 1 1

3569 1 1 1 1 2 1 1 1 1 195.7 1 1 1 1 1 0 1 1 1 1

3570 1 1 1 1 2 1 1 1 1 194.6 1 1 1 1 1 0 0 1 1 1

Group IV

– 20000 mg/m 3



# 4571 1 1 1 1 2 1 1 1 1 203.8 1 1 1 1 1 1 0 1 1 1

4572 1 1 1 1 2 1 1 1 1 181.2 1 1 1 1 1 0 0 1 1 1

4573 1 1 1 1 2 1 1 1 1 188.3 1 1 1 1 1 0 1 1 1 1

4574 1 1 1 1 1 1 1 1 1 197.9 1 1 1 1 1 0 0 1 1 1

4575 1 1 1 1 1 1 1 1 1 188.1 1 1 1 1 1 0 0 1 1 1

4 576 1 1 1 1 2 1 1 1 1 190.9 1 1 1 1 1 0 1 1 1 1

4577 1 1 1 1 2 1 1 1 1 187.2 1 1 1 1 1 0 0 1 1 1

4578 1 1 1 1 2 1 1 1 1 188.7 1 1 1 1 1 0 0 1 1 1

4579 1 1 1 1 2 1 1 1 1 209.5 1 1 1 1 1 0 0 1 1 1

4580 1 1 1 1 2 1 1 1 1 215.2 1 1 1 1 1 0 0 1 1 1

# Huntingdon Life Sciences 00

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- S Final Report

Females Individual Func tional Observational Battery Evaluations

Pretest Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Tria l 2 Mean

Group III

– 10000 mg/m 3



# 3561 1 1 1 1 2 1 835 945 890 705 510 608 1 7.3 5.8 6.6

3562 1 1 1 1 2 1 945 865 905 690 615 653 1 5.2 4.0 4.6

3563 1 1 1 1 2 1 720 885 803 550 625 588 1 6.2 5.3 5.8

356 4 1 1 1 1 2 1 985 1120 1053 500 420 460 1 5.1 5.0 5.1

3565 1 1 1 1 2 1 705 795 750 405 395 400 1 3.1 2.8 3.0

3566 1 1 1 1 2 1 1015 785 900 575 445 510 1 6.2 5.3 5.8

3567 1 1 1 1 2 1 965 780 873 580 575 578 1 5.3 5.5 5.4

3568 1 1 1 1 2 1 935 835 885 655 485 570 1 6.8 6.6 6.7

3569 1 1 1 1 2 1 885 1000 943 870 645 758 1 4.0 3.8 3.9

3570 1 1 1 1 2 1 1000 960 980 660 755 708 1 4.6 4.9 4.8

Group IV

– 20000 mg/m 3



# 4571 1 1 1 1 2 1 850 970 910 610 420 515 1 6 .1 5.9 6.0

4572 1 1 1 1 2 1 670 605 638 495 575 535 1 5.2 6.0 5.6

4573 1 1 1 1 2 1 950 1035 993 645 605 625 1 4.3 3.1 3.7

4574 1 1 1 1 2 1 945 1015 980 775 645 710 1 4.6 4.2 4.4

4575 1 1 1 1 2 1 1050 945 998 670 610 640 1 5.2 4.3 4.8

4576 1 1 1 1 2 1 1085 965 1025 535 705 620 1 6.2 6.8 6.5

4577 1 1 1 1 2 1 705 975 840 585 625 605 1 7.0 7.9 7.5

4578 1 1 1 1 1 1 570 845 708 690 605 648 1 5.8 5.3 5.6

4579 1 1 1 1 2 1 745 1075 910 620 465 543 1 4.1 4.0 4.1

4580 1 1 1 1 2 1 815 1075 945 705 920 813 1 5. 6 5.2 5.4

# Huntingdon Life Sciences 00

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Males Individual Functional Observational Battery Evaluations

4 th 

# week of exposure Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp

Number Post Vocal Close Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group I

- 0 mg/m 3



# 1071 1 1 1 1 2 1 1 1 1 448.3 1 1 1 1 1 0 0 1 1 1

1072 1 1 1 1 2 1 1 1 1 366.2 1 1 1 1 1 7 3 1 1 1

1073 1 1 1 1 2 1 1 1 1 397.1 1 1 1 1 1 0 0 1 1 1

1074 1 1 1 1 2 1 1 1 1 340.6 1 1 1 1 1 2 0 1 1 1

1075 1 1 1 1 2 1 1 1 1 372.8 1 1 1 1 1 0 0 1 1 1

1076 1 1 1 1 2 1 1 1 1 445.5 1 1 1 1 1 0 5 1 1 1

1077 1 1 1 1 2 1 1 1 1 407.0 1 1 1 1 1 0 0 1 1 1

1078 1 1 1 1 2 1 1 1 1 387.5 1 1 1 1 1 1 4 1 1 1

1079 1 1 1 1 2 1 1 1 1 405.9 1 1 1 1 1 0 0 1 1 1

1080 2 1 1 1 2 1 1 1 1 400.3 1 1 1 1 1 0 0 1 1 1

Group II

– 2000 mg/m 3



# 2061 1 1 1 1 2 1 1 1 1 360.9 1 1 1 1 1 0 0 1 1 1

2062 1 1 1 1 2 1 1 1 1 384.0 1 1 1 1 1 0 0 1 1 1

20 63 1 1 1 1 2 1 1 1 1 407.1 1 1 1 1 1 0 0 1 1 1

2064 1 1 1 1 2 1 1 1 1 345.6 1 1 1 1 1 5 5 1 1 1

2065 1 1 1 1 2 1 1 1 1 448.2 1 1 1 1 1 0 3 1 1 1

2066 1 1 1 1 2 1 1 1 1 419.2 1 1 1 1 1 0 5 1 1 1

2067 1 1 1 1 2 1 1 1 1 393.6 1 1 1 1 1 0 0 1 1 1

206 8 2 1 1 1 2 1 1 1 1 434.5 1 1 1 1 1 0 2 1 1 1

2069 1 1 1 1 2 1 1 1 1 425.0 1 1 1 1 1 0 0 1 1 1

2070 1 1 1 1 2 1 1 1 1 344.4 1 1 1 1 1 0 3 1 1 1

# Huntingdon Life Sciences 00

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Males Individual Functional Observational Battery Evaluations

4 th 

# week of exposure Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group I

- 0 mg/m 3



# 1071 1 1 1 1 2 1 1405 136 0 1383 765 705 735 1 7.7 6.8 7.3

1072 1 1 1 1 2 1 1380 1235 1308 755 960 858 1 6.0 5.2 5.6

1073 1 1 1 1 2 1 1495 1250 1373 785 1125 955 1 6.5 7.2 6.9

1074 1 1 1 1 2 1 1230 1585 1408 875 925 900 1 8.8 7.2 8.0

1075 1 1 1 1 2 1 1305 1225 1265 640 705 673 1 11.2 9.6 10.4

1076 1 1 1 1 2 1 1050 1335 1193 770 805 788 1 5.7 4.8 5.3

1077 1 1 1 1 2 1 1335 1180 1258 1030 835 933 1 9.8 8.7 9.3

1078 2 1 1 1 2 1 1345 1440 1393 925 1150 1038 1 7.3 5.6 6.5

1079 1 1 1 1 2 1 1110 1230 1170 835 810 823 1 7.6 6.3 7.0

1080 1 1 1 1 2 1 1010 900 955 740 550 645 1 5.4 5.1 5.3

Group II

– 2000 mg/m 3



# 2061 1 1 1 1 2 1 1480 1095 1288 580 635 608 1 7.0 7.3 7.2

2062 1 1 1 1 2 1 1440 1090 1265 825 785 805 1 7.6 7.7 7.7

2063 1 1 1 1 2 1 1505 1540 1523 1010 1280 1145 1 6.1 5.8 6.0

2064 1 1 1 1 2 1 1240 1230 1235 455 475 465 1 7.7 6.8 7.3

2065 1 1 1 1 1 1 1035 1235 1135 915 660 788 1 5.4 5.3 5.4

2066 1 1 1 1 2 1 1235 1265 1250 830 750 790 1 5.6 5.6 5.6

2067 1 1 1 1 2 1 1270 1520 1395 770 560 665 1 8.0 7.0 7.5

2068 1 1 1 1 2 1 1320 1670 1495 820 805 813 1 5.6 6.5 6.1

2069 1 1 1 1 2 1 1070 1315 1193 805 835 820 1 5.3 5.1 5.2

2070 1 1 1 1 2 1 1455 1535 1495 890 930 910 1 6.2 7.5 6.9

# H untingdon Life Sciences 00

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Males Individual Functional Observational Battery Evaluations

4 th 

# week of exposure Appendix H

a 

# Opens and closes mouth repeatedly. Home Cage Handling Open Field

Evaluation s Evaluations Evaluations

Animal Palp

Number Post Vocal Close Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group III

– 10000 mg/m 3



# 3061 1 1 1 2 2 1 1 1 1 418.2 1 1 1 1 1 3 0 1 1 1

3062 1 1 1 1 2 1 1 1 1 414.6 1 1 1 1 1 0 6 1 1 1

3063 1 1 1 1 2 1 1 1 1 395.0 1 1 1 1 1 5 1 1 1 1

3064 1 1 1 1 2 1 1 1 1 420.5 1 1 1 1 1 4 1 1 1 1

3065 2 1 1 1 2 1 1 1 1 345.9 1 1 1 1 1 0 0 1 1 1

3066 1 1 1 1 2 1 1 1 1 377.4 1 1 1 1 1 3 1 1 1 1

3067 1 1 1 2 2 1 1 1 1 451.6 1 1 1 1 1 5 0 1 1 1

3068 2 1 1 2 2 1 1 1 1 423.5 1 1 1 1 1 0 0 1 1 1

3069 1 1 1 1 2 1 1 1 1 431.7 1 1 1 1 1 0 0 1 1 1

3070 1 1 1 1 2 1 1 1 1 422.1 1 1 1 1 1 5 0 1 1 1

Group IV

– 20000 mg/m 3



# 4071 1 1 1 1 2 1 1 1 1 379.8 1 1 1 1 1 1 0 1 1 1

4072 1 1 1 1 2 1 1 1 1 430.1 1 1 1 1 1 6 0 1 1 1

4073 1 1 1 1 2 1 1 1 1 418.6 1 1 1 1 1 4 2 1 1 1

40 74 1 1 1 1 2 1 1 1 1 400.5 1 1 1 1 1 2 2 1 1 1

4075 1 1 1 1 2 1 1 1 1 368.4 1 1 1 1 1 0 2 1 1 1

4076 1 1 1 1 2 1 1 1 1 429.1 1 1 1 1 1 0 0 1 1 1

4077 1 1 1 1 2 1 1 1 1 379.1 1 1 1 1 1 0 0 1 1 1

4078 a 

# 2 1 1 1 2 1 1 1 1 424.4 1 1 1 1 1 0 0 1 1 1

4 079 1 1 1 1 2 1 1 1 1 424.2 1 1 1 1 1 0 2 1 1 1

4080 1 1 1 1 2 1 1 1 1 401.2 1 1 1 1 1 1 0 1 1 1

# Huntingdon Life Sciences 00

- 6130 Pa ge 451

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Males Individual Functional Observational Battery Evaluations

4 th 

# week of exposure Appendix H

a 

# Opens and closes mouth repeatedly. Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Numbe A pproach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group III

– 10000 mg/m 3



# 3061 1 1 1 1 2 1 1255 1315 1285 830 560 695 1 7.0 5.3 6.2

3062 1 1 1 1 2 1 810 850 830 1050 920 985 1 4.6 4.2 4.4

3063 1 1 1 1 2 1 1400 1470 1435 720 790 755 1 7.1 7.3 7.2

3064 1 1 1 1 2 1 1375 1400 1388 500 420 460 1 7.7 7.2 7.5

3065 1 1 1 1 2 1 1510 1710 1610 535 720 628 1 5.8 4.3 5.1

3066 1 1 1 1 2 1 1410 1585 1498 1130 795 963 1 8.1 7.7 7.9

3067 1 1 1 1 2 1 1490 1375 1433 1470 1280 1375 1 4.8 4.9 4.9

3068 1 1 1 1 2 1 1355 1475 1415 610 810 710 1 5.6 5.6 5.6

3069 1 1 1 1 2 1 1420 990 1205 680 820 750 1 8.3 8.1 8.2

3070 1 1 1 1 2 1 1220 1145 1183 1070 1265 1168 1 8.1 6.3 7.2

Group IV

– 20000 mg/m 3



# 4071 1 1 1 1 2 1 1325 1355 1340 595 685 640 1 7.2 6.8 7.0

4072 1 1 1 1 2 1 1265 1200 1233 1155 1175 1165 1 10.3 9.7 10.0

4073 1 1 1 1 2 1 1275 1155 1215 620 550 585 1 6.5 5.9 6.2

4074 1 1 1 1 2 1 1295 1310 1303 1165 1020 1093 1 5.8 5.9 5.9

4075 1 1 1 1 2 1 1135 1410 1273 700 845 773 1 5.7 3.7 4.7

4076 1 1 1 1 2 1 1335 890 1113 805 755 780 1 5.8 5.7 5.8

4077 1 1 1 1 2 1 1475 1255 1365 1195 840 1018 1 7.3 7.1 7.2

40 78 a 

# 1 1 1 1 2 1 1490 1665 1578 1010 1030 1020 1 5.2 6.3 5.8

4079 1 1 1 1 2 1 1440 1585 1513 1130 965 1048 1 10.3 10.2 10.3

4080 1 1 1 1 2 1 1285 1405 1345 870 565 718 1 7.2 6.6 6.9

# Huntingdon Life Sciences 00

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Females Individual Functional Observational Battery Evaluations

4 th 

# week of exposure Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluation s

Animal Palp

Number Post Vocal Close Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams

Group I

- 0 mg/m 3



# 1571 1 1 1 1 2 1 1 1 1 274.3 1 1 1 1 1 0 0 1 1 1

1572 1 1 1 1 2 1 1 1 1 265.4 1 1 1 1 1 0 0 1 1 1

1573 1 1 1 1 2 1 1 1 1 297.9 1 1 1 1 1 0 0 1 1 1

1574 1 1 1 1 2 1 1 1 1 276.7 1 1 1 1 1 0 0 1 1 1

1575 3 1 2 1 2 1 1 1 1 237.8 1 1 1 1 1 4 2 1 1 1

1576 1 1 1 1 2 1 1 1 1 264.2 1 1 1 1 1 0 0 1 1 1

1577 1 1 1 1 2 1 1 1 1 252.6 1 1 1 1 1 0 0 1 1 1

1578 2 1 1 1 2 1 1 1 1 279.5 1 1 1 1 1 0 0 1 1 1

1579 1 1 1 1 2 1 1 1 1 302.3 1 1 1 1 1 0 0 1 1 1

1580 2 1 1 1 2 1 1 1 1 264.3 1 1 1 1 1 0 0 1 1 1

Group II

– 2000 mg/m 3



# 2561 2 1 1 1 2 1 1 1 1 244.0 1 1 1 1 1 0 0 1 1 1

2562 1 1 1 1 2 1 1 1 1 293.5 1 1 1 1 1 0 0 1 1 1

2563 1 1 1 1 1 1 1 1 1 260.1 1 1 1 1 1 0 0 1 1 1

2564 2 1 1 1 2 1 1 1 1 260.1 1 1 1 1 1 0 0 1 1 1

2565 2 1 1 1 2 1 1 1 1 264.1 1 1 1 1 1 0 0 1 1 1

2566 1 1 1 1 2 1 1 1 1 215.9 1 1 1 1 1 0 0 1 1 1

2567 1 1 1 1 2 1 1 1 1 250.2 1 1 1 1 1 0 0 1 1 1

2568 3 1 2 1 2 1 1 1 1 291.3 1 1 1 1 1 0 0 1 1 1

2569 1 1 1 1 2 1 1 1 1 266.8 1 1 1 1 1 0 0 1 1 1

2570 1 1 1 1 2 1 1 1 1 277.0 1 1 1 1 1 0 0 1 1 1

# Huntingdon Life Sciences 00

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211

- DIPE

- S Final Report

Females Individual Functional Observational Battery Evaluations

4 th 

# week of exposure Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Respons e Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group I

- 0 mg/m 3



# 1571 1 1 1 1 2 1 1260 1140 1200 525 685 605 1 5.2 6.3 5.8

1572 1 1 1 1 2 1 1105 1020 1063 830 810 820 1 4.2 3.6 3.9

1573 1 1 1 1 1 1 1240 975 1108 760 730 745 1 3.9 4.3 4.1

1574 1 1 1 1 2 1 1145 1440 1293 765 710 738 1 5.7 5.1 5.4

1575 1 1 1 1 2 1 1105 1050 1078 805 505 655 1 6.0 5.4 5.7

1576 1 1 1 1 2 1 1000 1345 1173 855 1130 993 1 6.6 5.2 5.9

1 577 1 1 1 1 2 1 1125 1285 1205 795 875 835 1 6.0 4.9 5.5

1578 1 1 1 1 2 1 1115 1340 1228 660 655 658 1 6.1 6.7 6.4

1579 1 1 1 1 2 1 1245 1180 1213 930 855 893 1 6.2 5.0 5.6

1580 1 1 1 1 2 1 1365 1100 1233 955 910 933 1 6.6 5.9 6.3

Gro up II

– 2000 mg/m 3



# 2561 1 1 1 1 2 1 1225 1205 1215 720 840 780 1 4.3 4.8 4.6

2562 1 1 1 1 2 1 1130 970 1050 855 940 898 1 5.4 5.3 5.4

2563 1 1 1 1 2 1 1570 1050 1310 825 855 840 1 6.2 5.8 6.0

2564 1 1 1 1 2 1 1285 1175 1 230 895 955 925 1 3.5 4.8 4.2

2565 1 1 1 1 2 1 1235 1055 1145 695 665 680 1 6.1 5.7 5.9

2566 1 1 1 1 2 1 1305 1305 1305 530 625 578 1 5.0 4.6 4.8

2567 1 1 1 1 2 1 1290 1180 1235 785 805 795 1 6.2 7.0 6.6

2568 1 1 1 1 2 1 915 1040 978 455 525 490 1 5.2 4.5 4.9

2569 1 1 1 1 2 1 1145 1065 1105 800 835 818 1 4.8 4.4 4.6

2570 1 1 1 1 2 1 1040 1245 1143 900 835 868 1 5.1 5.8 5.5

# Huntingdon Life Sciences 00

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- S Final Report

Females Individual Functional Observational Battery Evaluations

4 th 

# week of exposure Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp

Number Post Vocal Cl ose Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group III

– 10000 mg/m 3



# 3561 1 1 1 1 2 1 1 1 1 248.4 1 1 1 1 1 0 0 1 1 1

3562 1 1 1 1 2 1 1 1 1 219.7 1 1 1 1 1 0 1 1 1 1

3563 2 1 1 1 2 1 1 1 1 236.5 1 1 1 1 1 1 1 1 1 1

3564 2 1 1 1 2 1 1 1 1 280.2 1 1 1 1 1 0 0 1 1 1

3565 1 1 1 1 2 1 1 1 1 306.6 1 1 1 1 1 0 0 1 1 1

3566 2 1 1 1 2 1 1 1 1 240.7 1 1 1 1 1 0 0 1 1 1

3567 2 1 1 1 2 1 1 1 1 237.7 1 1 1 1 1 0 0 1 1 1

3568 1 1 1 1 1 1 1 1 1 279.1 1 1 1 1 1 0 1 1 1 1

3569 1 1 1 1 1 1 1 1 1 240.8 1 1 1 1 1 0 0 1 1 1

3570 1 1 1 1 2 1 1 1 1 254.4 1 1 1 1 1 0 0 1 1 1

Group IV

– 20000 mg/m 3



# 4571 1 1 1 1 2 1 1 1 1 276.2 1 1 1 1 1 0 0 1 1 1

4572 1 1 1 1 2 1 1 1 1 258.9 1 1 1 1 1 0 0 1 1 1

4573 1 1 1 1 2 1 1 1 1 250.6 1 1 1 1 1 0 0 1 1 1

4574 1 1 1 1 2 1 1 1 1 240.6 1 1 1 1 1 0 0 1 1 1

4575 2 1 1 1 2 1 1 1 1 250.8 1 1 1 1 1 0 0 1 1 1

4576 2 1 1 2 2 1 1 1 1 237.9 1 1 1 1 1 0 0 1 1 1

4577 2 1 1 1 2 1 1 1 1 237.1 1 1 1 1 1 0 0 1 1 1

4578 1 1 1 1 2 1 1 1 1 250.5 1 1 1 1 1 0 0 1 1 1

4579 2 1 1 1 2 1 1 1 1 261.3 1 1 1 1 1 0 0 1 1 1

4580 2 1 1 1 2 1 1 1 1 268.6 1 1 1 1 1 0 0 1 1 1

# Huntingdon Life Sciences 00

- 6130 Page 455

211

- DIPE

- S Final Report

Females Individual Functional Observational Battery Evaluations

4 th 

# week of exposure Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Tria l 2 Mean Reflex Trial 1 Trial 2 Mean

Group III

– 10000 mg/m 3



# 3561 1 1 1 1 2 1 1295 1425 1360 890 975 933 1 7.2 6.8 7.0



# 3562 1 1 1 1 2 1 750 1000 875 785 660 723 1 6.2 4.1 5.2



# 3563 1 1 1 1 1 1 1250 1170 12 10 700 755 728 1 5.2 4.7 5.0



# 3564 1 1 1 1 2 1 1405 1145 1275 820 515 668 1 4.6 5.6 5.1



# 3565 1 1 1 1 2 1 1150 1385 1268 680 505 593 1 5.6 4.7 5.2



# 3566 1 1 1 1 2 1 845 885 865 830 830 830 1 4.6 4.1 4.4



# 3567 1 1 1 1 2 1 1120 1210 1165 875 750 813 1 5.2 4. 4 4.8



# 3568 1 1 1 1 2 1 1100 865 983 950 695 823 1 8.8 6.9 7.9



# 3569 1 1 1 1 2 1 1400 1320 1360 985 810 898 1 6.3 6.0 6.2



# 3570 1 1 1 1 2 1 1315 1420 1368 825 840 833 1 4.3 4.2 4.3



# Group IV

– 20000 mg/m 3



# 4571 1 1 1 1 2 1 1310 825 1068 580 485 533 1 7.8 8.6 8.2



# 4572 1 1 1 1 2 1 1335 1000 1168 520 755 638 1 6.6 6.5 6.6



# 4573 1 1 1 1 2 1 1040 1160 1100 610 610 610 1 4.7 4.0 4.4



# 4574 1 1 1 1 2 1 1215 1355 1285 675 730 703 1 5.2 4.6 4.9



# 4575 1 1 1 1 2 1 1220 1530 1375 815 870 843 1 6.2 5.1 5.7



# 4576 1 1 1 1 2 1 1495 1085 1290 1050 630 840 1 6.5 6.8 6.7



# 4577 1 1 1 1 2 1 1015 1285 1150 585 660 623 1 7.8 7.2 7.5



# 4578 1 1 1 1 2 1 1050 1035 1043 805 795 800 1 5.2 5.1 5.2



# 4579 1 1 1 1 2 1 1095 1205 1150 880 510 695 1 6.3 5.6 6.0



# 4580 1 1 1 1 2 1 1280 1145 1213 980 695 838 1 5.6 5.3 5.5

# Huntingdon Life Sciences 00

- 6130 Page 456

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- S Final Report

Males Individual Functional Observational Battery Eva luations

8 th 

# week of exposure Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp

Number Post Vocal Close Rem Hand Chromo Lac Sal Coat BW Gait Loco Ar ousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group I

- 0 mg/m 3



# 1071 1 1 1 1 2 1 1 1 1 569.1 1 1 1 1 1 1 0 1 1 1

1072 1 1 1 1 2 1 1 1 1 471.6 1 1 1 1 1 0 0 1 1 1

1073 2 1 1 2 2 1 1 1 1 479.4 1 1 1 1 1 0 0 1 1 1

1074 1 1 1 1 2 1 1 1 1 411.0 1 1 1 1 1 5 1 1 1 1

1075 1 1 1 2 2 1 1 1 1 454.2 1 1 1 1 1 3 0 1 1 1

1076 1 1 1 1 2 1 1 1 1 555.1 1 1 1 1 1 0 7 1 1 1

1077 2 1 1 1 2 1 1 1 1 482.3 1 1 1 1 1 0 0 1 1 1

1078 1 1 1 1 2 1 1 1 1 456.1 1 1 1 1 1 0 0 1 1 1

1079 1 1 1 2 2 1 1 1 1 476.6 1 1 1 1 1 0 0 1 1 1

1080 1 1 1 1 2 1 1 1 1 495.2 1 1 1 1 1 0 0 1 1 1

Group II

– 2000 mg/m 3



# 2061 1 1 1 1 2 1 1 1 1 432.8 1 1 1 1 1 2 0 1 1 1

2062 1 1 1 1 2 1 1 1 1 483.5 1 1 1 1 1 1 0 1 1 1

2063 1 1 1 1 2 1 1 1 1 514.2 1 1 1 1 1 0 0 1 1 1

2064 2 1 1 2 2 1 1 1 1 403.9 1 1 1 1 1 5 3 1 1 1

2065 2 1 1 1 2 1 1 1 1 546.9 1 1 1 1 1 0 3 1 1 1

2066 1 1 1 1 2 1 1 1 1 507.0 1 1 1 1 1 3 3 1 1 1

2067 1 1 1 1 2 1 1 1 1 470.1 1 1 1 1 1 0 0 1 1 1

2068 1 1 1 1 2 1 1 1 1 517.3 1 1 1 1 1 0 0 1 1 1

2069 1 1 1 1 2 1 1 1 1 502.7 1 1 1 1 1 0 0 1 1 1

2070 1 1 1 2 2 2 2 1 1 252.6 5 3 2 1 1 0 0 1 1 1

# Huntingdon Life Sciences 00

- 6130 Page 457

211

- DIPE

- S Final Report

Males Individual Functional Observational Battery Eva luations

8 th 

# week of exposure Appendix H

Reflex Grip Strength Landing Foot Sp lay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

G roup I

- 0 mg/m 3



# 1071 1 1 1 1 2 1 1445 1435 1440 780 825 803 1 7.5 8.2 7.9

1072 1 1 1 1 2 1 1245 1365 1305 870 850 860 1 5.1 5.1 5.1

1073 1 1 1 1 2 1 1310 850 1080 855 1840 1348 1 8.9 9.8 9.4

1074 1 1 1 1 2 1 1100 1120 1 110 820 935 878 1 7.5 8.0 7.8

1075 1 1 1 1 2 1 1025 975 1000 840 615 728 1 9.6 6.3 8.0

1076 1 1 1 1 2 1 1415 1025 1220 925 735 830 1 6.1 5.2 5.7

1077 1 1 1 1 2 1 1535 1005 1270 825 995 910 1 6.7 6.6 6.7

1078 1 1 1 1 2 1 1555 1400 1478 1105 1295 1200 1 4.9 4.7 4.8

1079 1 1 1 1 2 1 590 1300 945 830 870 850 1 9.6 9.7 9.7

1080 1 1 1 1 2 1 1260 1190 1225 960 660 810 1 6.7 5.5 6.1

Group II

– 2000 mg/m 3



# 2061 1 1 1 1 2 1 1245 1505 1375 890 900 895 1 7.4 8.6 8.0

2062 1 1 1 1 2 1 985 1050 1018 900 990 945 1 4.8 5.6 5.2

2063 1 1 1 1 2 1 1405 1455 1430 910 780 845 1 6.9 7.9 7.4

2064 1 1 1 1 2 1 1350 1180 1265 600 585 593 1 6.9 6.7 6.8

2065 1 1 1 1 2 1 1390 1295 1343 705 800 753 1 6.0 5.2 5.6

2066 1 1 1 1 2 1 1070 1080 1075 730 715 723 1 7.3 5.7 6.5

2067 1 1 1 1 2 1 655 1010 833 795 550 673 1 7.7 6.7 7.2

2068 1 1 1 1 2 1 1595 1340 1468 1355 965 1160 1 5.0 4.7 4.9

2069 1 1 1 1 2 1 1130 990 1060 635 580 608 1 5.6 5.7 5.7

2070 1 1 1 1 2 1 950 820 885 870 820 845 1 5.9 6.2 6.1

# Huntingdon Life Sciences 00

- 6130 Page 458

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- S Final Report

Males Individual Functional Observational Battery Evaluations

8 th 

# week of exposure Appendix H

a 

# Opens and closes mouth re peatedly. Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp

Numbe Post Vocal Close Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(gram s)

Group III

– 10000 mg/m 3



# 3061 1 1 1 1 2 1 1 1 1 519.2 1 1 1 1 1 3 0 1 1 1

3062 1 1 1 1 2 1 1 1 1 512.8 1 1 1 1 1 0 6 1 1 1

3063 1 1 1 1 2 1 1 1 1 492.6 1 1 1 1 1 0 3 1 1 1

3064 1 1 1 1 2 1 1 1 1 523.4 1 1 1 1 1 5 2 1 1 1

3065 1 1 1 1 2 1 1 1 1 429.3 1 1 1 1 1 1 0 1 1 1

3066 2 1 1 1 2 1 1 1 1 464.7 1 1 1 1 1 0 2 1 1 1

3067 1 1 1 1 2 1 1 1 1 546.4 1 1 1 1 1 0 0 1 1 1

3068 2 1 1 1 2 1 1 1 1 538.7 1 1 1 1 1 0 0 1 1 1

3069 1 1 1 1 2 1 1 1 1 504.3 1 1 1 1 1 0 0 1 1 1

3070 1 1 1 1 2 1 1 1 1 517.4 1 1 1 1 1 5 0 1 1 1

Group IV

– 20000 mg/m 3



# 4071 1 1 1 1 2 1 1 1 1 594.4 1 1 1 1 1 0 0 1 1 1

4072 1 1 1 1 2 1 1 1 1 536.3 1 1 1 1 1 0 0 1 1 1

4073 2 1 1 1 2 1 1 1 1 504.2 1 1 1 1 1 5 2 1 1 1

4074 1 1 1 1 2 1 1 1 1 466.6 1 1 1 1 1 4 0 1 1 1

4075 1 1 1 1 2 1 1 1 1 442.9 1 1 1 1 1 1 1 1 1 1

4076 2 1 1 1 2 1 1 1 1 521.8 1 1 1 1 1 0 0 1 1 1

4077 2 1 1 1 2 1 1 1 1 452.8 1 1 1 1 1 0 2 1 1 1

4078 a 

# 1 1 1 2 2 1 1 1 1 493.5 1 1 1 1 1 0 0 1 1 1

4079 2 1 1 2 2 1 1 1 1 518.1 1 1 1 1 1 0 0 1 1 1

4080 2 1 1 1 2 1 1 1 1 487.5 1 1 1 1 1 2 1 1 1 1

# Huntingdon Life Sciences 00

- 6130 Page 459

211

- DIPE

- S Final Report

Males Individual Functional Observational Battery Evaluations

8 th 

# week of exposure Appendix H

a 

# Opens and closes mouth re peatedly. Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group III

– 10000 mg/m 3



# 3061 1 1 1 1 2 1 1280 1295 1288 515 545 530 1 5.1 4.7 4.9

3062 1 1 1 1 2 1 1075 745 910 680 840 760 1 3.5 3.7 3.6

3063 1 1 1 1 2 1 1150 1310 1230 575 595 585 1 5.9 6.2 6.1

3064 1 1 1 1 2 1 1310 1210 1260 475 540 508 1 9.1 7.7 8.4

3065 1 1 1 1 2 1 1350 1375 1363 785 830 808 1 5.5 4.4 5.0

3066 1 1 1 1 2 1 1390 1370 1380 950 655 803 1 7.1 6.9 7.0

3067 1 1 1 1 2 1 1280 1245 1263 1070 1040 1055 1 7.4 5.7 6.6

3068 1 1 1 1 2 1 960 880 920 605 1015 810 1 5.3 5.1 5.2

3069 1 1 1 1 2 1 1295 850 1073 860 7 45 803 1 8.3 6.6 7.5

3070 1 1 1 1 2 1 930 460 695 1655 1795 1725 1 5.4 4.5 5.0

Group IV

– 20000 mg/m 3



# 4071 1 1 1 1 2 1 1520 1565 1543 630 660 645 1 7.2 7.1 7.2

4072 1 1 1 1 2 1 1535 1295 1415 1130 1045 1088 1 7.4 6.8 7.1

4073 1 1 1 1 2 1 1260 1125 1193 590 640 615 1 7.3 6.1 6.7

4074 1 1 1 1 2 1 610 1130 870 880 660 770 1 6.8 6.0 6.4

4075 1 1 1 1 2 1 1195 1270 1233 775 700 738 1 5.3 5.5 5.4

4076 1 1 1 1 2 1 975 1370 1173 885 805 845 1 6.0 5.9 6.0

407 7 1 1 1 1 2 1 1305 1275 1290 860 865 863 1 5.6 5.4 5.5

4078 a 

# 1 1 1 1 2 1 1420 1465 1443 835 1050 943 1 5.4 5.0 5.2

4079 1 1 1 1 2 1 1315 1430 1373 930 1040 985 1 6.0 6.3 6.2

4080 1 1 1 1 2 1 1360 900 1130 1025 1175 1100 1 6.7 7.9 7.3

# Huntingdon Life Sciences 00

- 6130 Page 460

211

- DIPE

- S Final Report

Females Individual Functional Observational Battery Evaluations

8 th 

# week of exposure Appendix H

Home Cage Han dling Open Field

Evaluations Evaluations Evaluations

Animal Palp

Numbe Post Vocal Close Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams

Group I

- 0 mg/m 3



# 1571 2 1 1 1 2 1 1 1 1 309.3 1 1 1 1 1 0 0 1 1 1

1572 1 1 1 1 2 1 1 1 1 296.6 1 1 1 1 1 0 0 1 1 1

1573 2 1 1 1 2 1 1 1 1 339.7 1 1 1 1 1 0 0 1 1 1

1574 1 1 1 1 2 1 1 1 1 321.6 1 1 1 1 1 0 0 1 1 1

1575 3 1 2 1 2 1 1 1 1 253.4 1 1 1 1 1 0 3 1 1 1

1576 1 1 1 1 2 1 1 1 1 285.6 1 1 1 1 1 0 0 1 1 1

1577 2 1 1 1 2 1 1 1 1 275.9 1 1 1 1 1 0 0 1 1 1

1578 2 1 1 1 2 1 1 1 1 304.3 1 1 1 1 1 0 0 1 1 1

1579 1 1 1 1 2 1 1 1 1 350.4 1 1 1 1 1 0 0 1 1 1

1580 2 1 1 1 2 1 1 1 1 288.4 1 1 1 1 1 0 0 1 1 1

Group II

– 2000 mg/m 3



# 2561 2 1 1 1 2 1 1 1 1 265.1 1 1 1 1 1 0 0 1 1 1

2562 2 1 1 1 2 1 1 1 1 323.7 1 1 1 1 1 0 0 1 1 1

2563 2 1 1 1 2 1 1 1 1 289.6 1 1 1 1 1 0 0 1 1 1

2564 2 1 1 1 2 1 1 1 1 291.3 1 1 1 1 1 0 0 1 1 1

2565 1 1 1 1 2 1 1 1 1 293.7 1 1 1 1 1 0 0 1 1 1

2566 1 1 1 1 2 1 1 1 1 229.8 1 1 1 1 1 0 0 1 1 1

2567 1 1 1 1 2 1 1 1 1 285.6 1 1 1 1 1 2 2 1 1 1

2568 2 1 1 1 2 1 1 1 1 316.5 1 1 1 1 1 0 0 1 1 1

2569 1 1 1 1 2 1 1 1 1 288.4 1 1 1 1 1 0 0 1 1 1

2570 1 1 1 1 2 1 1 1 1 304.1 1 1 1 1 1 0 0 1 1 1

# Huntingdon Life Sciences 00

- 6130 Page 461

211

- DIPE

- S Final Report

Females Individual Functional Observational Battery Evaluations

8 th 

# week of exposure Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group I

- 0 mg/m 3



# 1571 1 1 1 1 2 1 1380 1050 1215 690 900 795 1 5.6 4.7 5.2

1572 1 1 1 1 2 1 1135 1050 1093 715 895 805 1 3.9 4.5 4.2

1573 1 1 1 1 2 1 1185 1185 1185 575 590 583 1 4.6 4.5 4.6

1574 1 1 1 1 2 1 1125 955 1040 870 900 885 1 5.8 5.6 5.7

1575 1 1 1 1 2 1 930 845 888 585 550 568 1 5.5 5.2 5.4

1576 1 1 1 1 2 1 1405 1180 1293 785 775 780 1 5.5 4.7 5.1

1577 1 1 1 1 2 1 1245 1330 1288 560 570 565 1 4.9 4.4 4.7

1578 1 1 1 1 2 1 1080 1490 1285 880 825 853 1 4.7 4.8 4.8

1579 1 1 1 1 2 1 905 1445 1175 750 885 818 1 5.7 4.1 4.9

1580 1 1 1 1 2 1 1410 1055 1233 1020 1175 1098 1 6.1 4.4 5.3

Group II

– 2000 mg/m 3



# 2561 1 1 1 1 2 1 1145 1055 1100 400 760 580 1 4.1 4.2 4.2

2562 1 1 1 1 2 1 880 895 888 985 950 968 1 4.8 3.7 4.3

2563 1 1 1 1 2 1 1070 660 865 815 805 8 10 1 5.7 5.2 5.5

2564 1 1 1 1 2 1 995 1480 1238 830 1040 935 1 4.2 3.9 4.1

2565 1 1 1 1 2 1 970 985 978 840 635 738 1 6.4 6.3 6.4

2566 1 1 1 1 1 1 1100 1045 1073 530 500 515 1 5.1 4.1 4.6

2567 1 1 1 1 2 1 1300 1050 1175 880 985 933 1 5.1 6.9 6.0

2568 1 1 1 1 2 1 1195 495 845 830 720 775 1 5.1 5.0 5.1

2569 1 1 1 1 2 1 865 950 908 640 835 738 1 4.8 5.2 5.0

2570 1 1 1 1 2 1 1145 1315 1230 675 845 760 1 5.5 4.8 5.2

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Females Individual Functional Observational Battery Evaluations

8 th 

# week of exposure Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp

Number Post Vocal Close Rem Hand Chromo Lac Sa Coat BW Gai Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group III

– 10000 mg/m 3



# 3561 2 1 1 1 2 1 1 1 1 279. 1 1 1 1 1 1 0 0 1 1 1

3562 1 1 1 1 2 1 1 1 1 234.5 1 1 1 1 1 0 0 1 1 1

3563 1 1 1 1 2 1 1 1 1 254.4 1 1 1 1 1 0 0 1 1 1

3564 1 1 1 1 2 1 1 1 1 309.1 1 1 1 1 1 0 0 1 1 1

3565 2 1 1 1 2 1 1 1 1 351.7 1 1 1 1 1 0 0 1 1 1

3566 2 1 1 1 2 1 1 1 1 273.1 1 1 1 1 1 0 0 1 1 1

3567 2 1 1 1 2 1 1 1 1 258.5 1 1 1 1 1 0 0 1 1 1

3568 1 1 1 1 2 1 1 1 1 305.6 1 1 1 1 1 0 0 1 1 1

3569 1 1 1 1 2 1 1 1 1 262.3 1 1 1 1 1 0 0 1 1 1

3570 1 1 1 1 2 1 1 1 1 274.6 1 1 1 1 1 0 0 1 1 1

Group I V

– 20000 mg/m 3



# 4571 1 1 1 1 2 1 1 1 1 324.3 1 1 1 1 1 0 0 1 1 1

4572 1 1 1 1 2 1 1 1 1 282.2 1 1 1 1 1 0 0 1 1 1

4573 1 1 1 1 2 1 1 1 1 282.5 1 1 1 1 1 0 0 1 1 1

4574 2 1 1 1 2 1 1 1 1 266.4 1 1 1 1 1 0 0 1 1 1

4575 2 1 1 1 2 1 1 1 1 292.9 1 1 1 1 1 0 0 1 1 1

4576 2 1 1 1 2 1 1 1 1 262.1 1 1 1 1 1 0 0 1 1 1

4577 1 1 1 1 2 1 1 1 1 262.9 1 1 1 1 1 0 0 1 1 1

4578 1 1 1 1 2 1 1 1 1 269.5 1 1 1 1 1 0 0 1 1 1

4579 2 1 1 1 2 1 1 1 1 288.7 1 1 1 1 1 0 0 1 1 1

4580 2 1 1 1 2 1 1 1 1 291.3 1 2 4 1 1 0 0 1 1 1

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Females Individual Functional Observational Battery Evaluations

8 th 

# week of exposure Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex P ropri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group III

– 10000 mg/m 3



# 3561 1 1 1 1 2 1 955 845 900 900 900 900 1 6.1 5.2 5.7

3562 1 1 1 1 2 1 1245 1185 1215 680 505 593 1 5.0 4.7 4.9

3563 1 1 1 1 2 1 1490 1340 1415 595 915 755 1 5.2 3.6 4.4

3564 1 1 1 1 2 1 940 1370 1155 810 550 680 1 3.3 3.8 3.6

3565 1 1 1 1 2 1 1310 1255 1283 805 960 883 1 4.0 4.7 4.4

3566 1 1 1 1 2 1 1255 1285 1270 700 840 770 1 4.1 3.8 4.0

3567 1 1 1 1 2 1 760 1200 980 600 885 743 1 4.8 4.7 4.8

3568 1 1 1 1 2 1 990 930 960 985 710 848 1 6.2 5.6 5.9

3569 1 1 1 1 2 1 1440 1405 1423 805 1000 903 1 5.8 6.7 6.3

3570 1 1 1 1 2 1 775 995 885 1035 890 963 1 4.6 4.5 4.6

Group IV

– 20000 mg/ m 3



# 4571 1 1 1 1 2 1 715 1195 955 615 465 540 1 7.1 6.7 6.9

4572 1 1 1 1 2 1 865 795 830 750 610 680 1 6.2 5.4 5.8

4573 1 1 1 1 2 1 1090 1335 1213 915 515 715 1 4.0 4.1 4.1

4574 1 1 1 1 2 1 750 920 835 690 615 653 1 5.3 5 .0 5.2

4575 1 1 1 1 2 1 805 1215 1010 935 705 820 1 5.0 5.1 5.1

4576 1 1 1 1 2 1 1355 1110 1233 725 610 668 1 6.2 4.6 5.4

4577 1 1 1 1 2 1 1150 1390 1270 840 645 743 1 6.2 7.5 6.9

4578 1 1 1 1 2 1 810 845 828 840 1085 963 1 5.1 4.3 4.7

4579 1 1 1 1 2 1 1085 695 890 885 505 695 1 5.7 5.3 5.5

4580 1 1 1 1 2 1 1530 705 1118 995 880 938 1 5.3 6.6 6.0

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Males Individual Functional Observational Battery Evaluations

13 th 

# week of exposure Appendix H

a 

# Humane sacrificed. Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp

Numbe Post Vocal Close Rem Hand Chromo Lac Sa Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group I

- 0 mg/m 3



# 1071 1 1 1 1 2 1 1 1 1 665.3 1 1 1 1 1 0 0 1 1 1

1072 1 1 1 1 2 1 1 1 1 543.7 1 1 1 1 1 6 3 1 1 1

1073 2 1 1 3 3 1 1 1 1 547.3 1 1 1 1 1 0 0 1 1 1

1074 1 1 1 1 2 1 1 1 1 464.1 1 1 1 1 1 4 3 1 1 1

1075 1 1 1 1 2 1 1 1 1 511.3 1 1 1 1 1 0 0 1 1 1

1076 1 1 1 1 2 1 1 1 1 635.0 1 1 1 1 1 0 4 1 1 1

1077 1 1 1 1 2 1 1 1 1 524.7 1 1 1 1 1 0 0 1 1 1

1078 1 1 1 1 2 1 1 1 1 524.6 1 1 1 1 1 0 1 1 1 1

1079 1 1 1 1 2 1 1 1 1 550.9 1 1 1 1 1 0 0 1 1 1

1080 2 1 1 1 2 1 1 1 1 588.9 1 1 1 1 1 0 2 1 1 1

Group II

– 2000 mg/m 3



# 2061 2 1 1 1 2 1 1 1 1 503.2 1 1 1 1 1 0 0 1 1 1

2062 1 1 1 1 2 1 1 1 1 552.4 1 1 1 1 1 0 0 1 1 1

2063 1 1 1 1 2 1 1 1 1 582.5 1 1 1 1 1 0 0 1 1 1

2064 1 1 1 1 2 1 1 1 1 462.5 1 1 1 1 1 6 4 1 1 1

2065 2 1 1 1 1 1 1 1 1 611.6 1 1 1 1 1 5 7 1 1 1

2066 1 1 1 1 2 1 1 1 1 589.0 1 1 1 1 1 4 0 1 1 1

2067 1 1 1 1 2 1 1 1 1 532.6 1 1 1 1 1 7 0 1 1 1

2068 2 1 1 2 2 1 1 1 1 601.1 1 1 1 1 1 2 0 1 1 1

2069 1 1 1 1 2 1 1 1 1 577.9 1 1 1 1 1 0 0 1 1 1

2070 a a a a a a a a a a a a a a a a a a a a

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Males Individual Functional Observational Battery Evaluations

13 th 

# week of exposure Append ix H

a 

# Humane sacrificed. Reflex Grip Strengt h Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approac Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group I

- 0 mg/m 3



# 1071 1 1 1 1 2 1 1830 1795 1813 1145 1345 1245 1 7.1 7.2 7.2

1072 1 1 1 1 2 1 1370 1000 1185 880 970 925 1 4.7 5.4 5.1

1073 1 1 1 1 2 1 1565 1400 1483 1440 1540 1490 1 7.7 8.0 7.9

1074 1 1 1 1 2 1 970 490 730 1025 715 870 1 7.5 6.7 7.1

1075 1 1 1 1 2 1 1050 1115 1083 835 745 790 1 10.5 7.9 9.2

1076 1 1 1 1 2 1 1080 1085 1083 830 905 868 1 4.5 4.2 4.4

1077 3 1 1 1 2 1 1340 1500 1420 770 840 805 1 7.0 6.4 6.7

1078 1 1 1 1 2 1 1390 1355 1373 1225 1105 1165 1 4.6 3.9 4.3

1079 1 1 1 1 2 1 1350 1550 1450 890 1030 960 1 5.6 4.7 5.2

1080 1 1 1 1 2 1 1295 1165 1230 945 880 913 1 6.8 5.4 6.1

Group II

– 2000 mg/m 3



# 2061 1 1 1 1 2 1 1165 1070 11 18 1225 1135 1180 1 6.4 6.2 6.3

2062 1 1 1 1 2 1 1295 1170 1233 1095 1220 1158 1 7.1 6.8 7.0

2063 1 1 1 1 2 1 1480 1555 1518 805 920 863 1 6.6 9.4 8.0

2064 1 1 1 1 2 1 1395 1025 1210 790 625 708 1 6.8 6.4 6.6

2065 1 1 1 1 2 1 815 930 873 915 910 913 1 5.3 5.2 5.3

2066 1 1 1 1 2 1 925 1005 965 1040 790 915 1 6.3 5.2 5.8

2067 1 1 1 1 2 1 1185 1155 1170 685 930 808 1 5.3 5.4 5.4

2068 1 1 1 1 2 1 1535 1575 1555 1390 1255 1323 1 7.7 5.8 6.8

2069 1 1 1 1 2 1 1025 1230 1128 620 695 658 1 4.8 4.2 4.5

2070 a a a a a a a a a a a a a a a a

# Huntingdon Life Sciences 00

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- S Final Report

Males Individual Functional Observational Battery Evaluations

13 th 

# week of exposure Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp

Numbe Post Vocal Clos Rem Hand Chromo Lac Sa Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group III

– 10000 mg/m 3



# 3061 1 1 1 1 2 1 1 1 1 599.2 1 1 1 1 1 0 0 1 1 1

3062 1 1 1 1 2 1 1 1 1 597.1 1 1 1 1 1 0 7 1 1 1

3063 2 1 1 1 2 1 1 1 1 567.0 1 1 1 1 1 4 0 1 1 1

3064 1 1 1 1 2 1 1 1 1 594.3 1 1 1 1 1 2 0 1 1 1

3065 1 1 1 1 2 1 1 1 1 476.5 1 1 1 1 1 0 0 1 1 1

3066 1 1 1 1 2 1 1 1 1 533.5 1 1 1 1 1 0 0 1 1 1

3067 1 1 1 1 2 1 1 1 1 617.8 1 1 1 1 1 0 0 1 1 1

3068 1 1 1 2 2 1 1 1 1 626.7 1 1 1 1 1 0 0 1 1 1

3069 1 1 1 1 2 1 1 1 1 567.0 1 1 1 1 1 0 0 1 1 1

3070 1 1 1 1 2 1 1 1 1 601.7 1 1 1 1 1 7 0 1 1 1

Group IV

– 20000 mg/m 3



# 4071 1 1 1 1 2 1 1 1 1 515.7 1 1 1 1 1 0 0 1 1 1

4072 1 1 1 1 2 1 1 1 1 6 22.5 1 1 1 1 1 0 1 1 1 1

4073 1 1 1 1 2 1 1 1 1 569.2 1 1 1 1 1 0 0 1 1 1

4074 1 1 1 1 2 1 1 1 1 433.7 1 1 1 1 1 0 0 1 1 1

4075 1 1 1 1 2 1 1 1 1 517.8 1 1 1 1 1 0 1 1 1 1

4076 2 1 1 2 2 1 1 1 1 593.2 1 1 1 1 1 0 0 1 1 1

4077 2 1 1 1 2 1 1 1 1 51 4.2 1 1 1 1 1 3 3 1 1 1

4078 2 1 1 2 2 1 1 1 1 566.3 1 1 1 1 1 3 0 1 1 1

4079 1 1 1 3 3 1 1 1 1 585.0 1 1 1 1 1 0 0 1 1 1

4080 2 1 1 2 2 1 1 1 1 539.5 1 1 1 1 1 0 0 1 1 1

# Huntingdon Life Sciences 00

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Males Individual Functional Observational Battery Evaluations

13 th 

# week of exposure Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimet ers)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group III

– 10000 mg/m 3



# 3061 1 1 1 1 2 1 975 1300 1138 750 475 613 1 5.3 5.2 5.3

3062 1 1 1 1 2 1 1740 1000 1370 940 675 808 1 4.5 3.7 4.1

3063 2 1 1 1 2 1 1245 955 1100 1410 1340 1375 1 7.3 7.4 7.4

3064 1 1 1 1 2 1 1245 1520 1383 660 670 665 1 7.9 7.8 7.9

306 5 1 1 1 1 2 1 1315 1220 1268 735 700 718 1 5.9 4.6 5.3

3066 1 1 1 1 2 1 1830 1020 1425 1370 1040 1205 1 6.2 5.3 5.8

3067 1 1 1 1 2 1 1475 915 1195 1290 1165 1228 1 4.3 5.3 4.8

3068 1 1 1 1 2 1 675 1130 903 990 1075 1033 1 6.4 6.1 6.3

3069 1 1 1 1 2 1 1 375 850 1113 1005 485 745 1 6.3 4.3 5.3

3070 1 1 1 1 2 1 840 950 895 1390 1685 1538 1 5.0 5.3 5.2

Group IV

– 20000 mg/m 3



# 4071 1 1 1 1 2 1 1235 1340 1288 670 435 553 1 5.6 6.3 6.0

4072 1 1 1 1 2 1 1100 1 235 1168 1485 1505 1495 1 5.8 4.9 5.4

4073 1 1 1 1 2 1 1195 890 1043 680 685 683 1 7.6 6.3 7.0

4074 1 1 1 1 2 1 1020 1175 1098 785 740 763 1 7.8 6.9 7.4

4075 1 1 1 1 2 1 1135 1235 1185 705 705 705 1 5.1 4.6 4.9

4076 1 1 1 1 2 1 1295 1570 1433 930 1160 1045 1 5.5 3.6 4.6

4077 1 1 1 1 2 1 1240 1095 1168 880 1100 990 1 4.9 5.2 5.1

4078 1 1 1 1 2 1 1925 1635 1780 320 655 488 1 5.5 4.1 4.8

4079 1 1 1 1 2 1 1525 1550 1538 1225 1250 1238 1 5.2 4.4 4.8

4080 1 1 1 1 2 1 1375 1415 1395 1020 1290 1155 1 6.8 7. 5 7.2

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Females Individual Functional Observational Battery Evaluations

13 th 

# week of exposure Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp

Numbe Post Vocal Clos Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams

Group I

- 0 mg/m 3



# 1571 1 1 1 1 2 1 1 1 1 329.8 1 1 1 1 1 0 0 1 1 1

1572 1 1 1 1 2 1 1 1 1 329.9 1 1 1 1 1 0 0 1 1 1

1573 2 1 1 1 2 1 1 1 1 376.2 1 1 1 1 1 0 1 1 1 1

1574 1 1 1 1 1 1 1 1 1 345.5 1 1 1 1 1 0 0 1 1 1

1575 1 1 1 1 2 1 1 1 1 272.8 1 1 1 1 1 3 1 1 1 1

1576 1 1 1 1 2 1 1 1 1 308.8 1 1 1 1 1 0 0 1 1 1

1577 1 1 1 1 2 1 1 1 1 296.2 1 1 1 1 1 0 0 1 1 1

1578 1 1 1 1 2 1 1 1 1 331.5 1 1 1 1 1 1 2 1 1 1

1579 1 1 1 1 2 1 1 1 1 392.9 1 1 1 1 1 2 2 1 1 1

1580 2 1 1 1 2 1 1 1 1 304.0 1 1 1 1 1 0 0 1 1 1

Group II

– 2000 mg/m 3



# 2561 2 1 1 1 2 1 1 1 1 275.3 1 1 1 1 1 0 0 1 1 1

2562 2 1 1 1 2 1 1 1 1 352.2 1 1 1 1 1 0 0 1 1 1

2563 1 1 1 1 1 1 1 1 1 317.6 1 1 1 1 1 0 1 1 1 1

2564 2 1 1 1 2 1 1 1 1 320.7 1 1 1 1 1 0 1 1 1 1

2565 2 1 1 1 2 1 1 1 1 325.3 1 1 1 1 1 0 1 1 1 1

2566 1 1 1 1 2 1 1 1 1 255.1 1 1 1 1 1 0 0 1 1 1

2567 1 1 1 2 2 1 1 1 1 305.0 1 1 1 1 1 0 0 1 1 1

2568 2 1 1 1 2 1 1 1 1 341.0 1 2 4 1 1 0 0 1 1 1

2569 2 1 1 1 2 1 1 1 1 321.3 1 1 1 1 1 0 0 1 1 1

2570 2 1 1 1 2 1 1 1 1 327.9 1 1 1 1 1 0 0 1 1 1

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- S Final Report

Females Individual Functional Observational Battery Evaluations

13 th 

# week of exposure Appendix H

a 

# Slight tremors upon handling. Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pin na Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group I

- 0 mg/m 3



# 1571 1 1 1 1 2 1 1400 1395 1398 1135 1085 1110 1 4.2 3.6 3.9

1572 1 1 1 1 2 1 1080 1600 1340 910 890 900 1 3.9 3.2 3.6

1573 1 1 1 1 2 1 1065 990 1028 575 660 618 1 4.2 4.6 4.4

1574 1 1 1 1 2 1 815 930 873 755 750 753 1 4.1 4.3 4.2

1575 1 1 1 1 2 1 675 875 775 725 785 755 1 6.0 5.8 5.9

1576 1 1 1 1 2 1 1095 1300 1198 765 865 815 1 4.2 3.9 4.1

1577 1 1 1 1 2 1 1055 1365 1210 870 800 835 1 3.6 3.4 3.5

1578 1 1 1 1 2 1 1535 665 1100 685 950 818 1 4.6 4.1 4.4

1579 1 1 1 1 2 1 670 1075 873 735 1050 893 1 4.4 3.2 3.8

1580 1 1 1 1 2 1 1260 1010 1135 1260 1305 1283 1 4.3 3.4 3.9

Group II

– 2000 mg/m 3



# 2561 1 1 1 1 2 1 850 975 913 815 830 823 1 3.5 4.2 3.9

2562 1 1 1 1 2 1 1010 1390 1200 860 855 858 1 5.6 3.5 4.6

2563 1 1 1 1 2 1 580 1525 1053 1005 915 960 1 4.7 4.5 4.6

2564 1 1 1 1 2 1 815 1115 965 1220 1000 1110 1 3.0 3.9 3.5

2565 1 1 1 1 2 1 1145 640 893 635 455 545 1 6.9 5.8 6.4

2566 1 1 1 1 2 1 1270 1210 1240 730 630 680 1 3.3 3.7 3.5

2567 1 1 1 1 2 1 935 560 748 560 67 0 615 1 a 

# 5.6 5.4 5.5

2568 1 1 1 1 2 1 740 850 795 520 750 635 1 5.3 4.4 4.9

2569 1 1 1 1 2 1 1145 1000 1073 800 615 708 1 4.6 4.1 4.4

2570 1 1 1 1 2 1 1515 1185 1350 730 1050 890 1 4.7 5.2 5.0

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Females Individual Functional Observational Battery Evaluations

13 th 

# week of exposure Appendix H

Home Cage Handling Open Field

Evaluations Evaluatio ns Evaluations

Animal Palp

Numbe Post Vocal Clos Rem Han Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group III

– 10000 mg/m 3



# 3561 1 1 1 1 2 1 1 1 1 313.6 1 1 1 1 1 0 0 1 1 1

3562 1 1 1 1 2 1 1 1 1 263.7 1 1 1 1 1 0 3 1 1 1

3563 1 1 1 1 2 1 1 1 1 263.3 1 1 1 1 1 0 0 1 1 1

3564 1 1 1 1 2 1 1 1 1 334.9 1 1 1 1 1 0 0 1 1 1

3565 1 1 1 1 1 1 1 1 1 379.6 1 1 1 1 1 0 0 1 1 1

3566 1 1 1 1 2 1 1 1 1 298.8 1 1 1 1 1 0 0 1 1 1

3567 2 1 1 1 2 1 1 1 1 292.0 1 1 1 1 1 0 0 1 1 1

3568 1 1 1 1 2 1 1 1 1 317.6 1 1 1 1 1 0 0 1 1 1

3569 1 1 1 1 2 1 1 1 1 273.5 1 1 1 1 1 0 1 1 1 1

3570 1 1 1 1 2 1 1 1 1 309.8 1 1 1 1 1 4 1 1 1 1

Group IV

– 20000 mg/m 3



# 4571 1 1 1 1 2 1 1 1 1 356.4 1 1 1 1 1 0 1 1 1 1

4572 1 1 1 1 2 1 1 1 1 309.3 1 1 1 1 1 0 0 1 1 1

4573 1 1 1 1 2 1 1 1 1 309.8 1 1 1 1 1 0 0 1 1 1

4574 1 1 1 1 1 1 1 1 1 282.1 1 1 1 1 1 0 0 1 1 1

4575 2 1 1 1 2 1 1 1 1 312.5 1 1 1 1 1 0 0 1 1 1

4576 2 1 1 1 2 1 1 1 1 280.3 1 1 1 1 1 0 3 1 1 1

4577 2 1 1 1 2 1 1 1 1 287.2 1 1 1 1 1 0 0 1 1 1

4578 1 1 1 1 2 1 1 1 1 279.8 1 1 1 1 1 0 0 1 1 1

4579 1 1 1 1 2 1 1 1 1 319.0 1 1 1 1 1 0 0 1 1 1

4580 2 1 1 1 2 1 1 1 1 312.2 1 2 4 1 1 0 0 1 1 1

# Huntingdon Life Sciences 00

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Females Individual Functional Observational Battery Evaluations

13 th 

# week of exposure Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approa ch Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group III

– 10000 mg/m 3



# 3561 1 1 1 1 2 1 825 1010 918 625 865 745 1 4.8 3.6 4.2

3562 1 1 1 1 2 1 1 280 1245 1263 1090 1135 1113 1 5.2 3.9 4.6

3563 1 1 1 1 2 1 1015 1215 1115 725 535 630 1 4.1 3.2 3.7

3564 1 1 1 1 2 1 1030 1180 1105 900 900 900 1 4.0 4.9 4.5

3565 1 1 1 1 2 1 1265 940 1103 800 610 705 1 4.7 5.5 5.1

3566 1 1 1 1 2 1 1420 1020 1220 645 905 775 1 5.7 3.6 4.7

3567 1 1 1 1 2 1 1190 1570 1380 985 935 960 1 4.6 5.0 4.8

3568 1 1 1 1 2 1 1255 985 1120 900 725 813 1 5.6 5.3 5.5

3569 1 1 1 1 2 1 1190 1245 1218 1005 830 918 1 4.8 4.5 4.7

3570 1 1 1 1 2 1 1175 950 1063 965 910 938 1 5.4 4.7 5.1

Group IV

– 20000 mg/m 3



# 4571 1 1 1 1 2 1 765 740 753 590 820 705 1 5.6 5.7 5.7

4572 1 1 1 1 2 1 695 1390 1043 900 800 850 1 5.3 4.5 4.9

4573 1 1 1 1 2 1 1165 895 1030 795 775 785 1 5.6 4.6 5.1

4574 1 1 1 1 2 1 1520 1285 1403 650 800 725 1 4.9 4.6 4.8

4575 1 1 1 1 2 1 950 980 965 855 805 830 1 4.4 3.8 4.1

4576 1 1 1 1 2 1 1165 1295 1230 830 875 853 1 4.6 6.2 5.4

4577 1 1 1 1 2 1 1030 895 963 620 720 670 1 4.6 4.7 4.7

4578 1 1 1 1 2 1 850 940 895 895 1080 988 1 4.8 4.2 4.5

4579 1 1 1 1 2 1 1290 805 1048 830 820 825 1 4.3 5.1 4.7

4580 1 1 1 1 1 1 1030 1075 1053 800 825 813 1 5.7 5.4 5.6

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Individual Hematology Values

Preface Appendix I

Reporting

Abbreviation Param eter Units

HGB Hemoglobin Concentration g/dL

HCT Hematocrit percent

RBC Erythrocyte Count 10 6 

# / μ L

RETIC Absolute Reticulocyte Count 10 9 

# /L

PLT Platelet Count 10 3 

# / μ L

MCV Mean Corpuscular Volume fL

MCH Mean Corpuscular Hemoglobin pg

MCHC Mean Corpuscular Hemoglobin

Co ncentration g/dL

WBC Total Leukocyte Count 10 3 

# / μ L

ANEU Absolute Neutrophils 10 3 

# / μ L

ALYM Absolute Lymphocytes 10 3 

# / μ L

AMONO Absolute Monocytes 10 3 

# / μ L

AEOS Absolute Eosinophils 10 3 

# / μ L

ABASO Absolute Basophils 10 3 

# / μ L

ALUC Absolute Large Unstained Cells 10 3 

# / μ L

ERYTHROCYTE MORPHOLOGY

Abbreviation Cell Morphology Range

Poly Polychromasia 1+

= few

Poik Poikilocytosis 2+

= slight

NAN

= No

Abnormalities

Noted

Key to Other Abbreviations

CS

= Clotted Specimen

NS

= No Specimen

Notes:

1. Animal 2060 at termination had no specimen because it was transferred to the Satellite

Group.

2. Manual differential values were reported for Animal Nos. 1063M (Week 4), 1068M,

4062M, 1568F, 2559F, 4563F, 4569F (Termination), and 1057M (R ecovery ) and

therefore the value for ALUC is presented as 0

.HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-Week4Group1M0mg/m(3)AnimalNumber1061106210631064106510661067106810691070MeanSDnHGBg/dL16.516.716.216.015.816.315.116.215.215.516.00.5410HCT%49.650.249.748.446.449.547.349.346.447.548.41.4310RBC9.008.898.558.628.428.788.628.918.368.048.620.29410RETICPLTMCVMCHMCHCWBCfLpgg/dL188.292255.118.333.213.20212.396056.518.733.216.98187.984058.119.032.716.64199.093056.118.633.117.43137.173855.118.734.018.69234.691356.418.633.015.54193.189954.817.632.012.25225.075355.318.232.915.21200.7125855.518.232.915.38246.080959.019.332.718.70202.490256.218.533.016.0030.41146.41.390.470.502.136101010101010Page 473HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-Week4Group1Momg/m(3)AnimalNumber1061106210631064106510661067106810691070MeanSDnANEUX10A3/uL1.632.761.661.572.122.631.681.692.041.651.940.43710ALYMX10A3/uL10.8613.3814.1415.0215.6612.099.9412.6112.3816.0613.212.02210AMONOX10A3/uL0.400.480.670.320.350.500.340.360.380.430.420.10510AEOSABASOALUCPoikPolyX10A3/ULX10A3/uLX10A3/uL0.120.120.07NAN1+0.090.180.10NAN1+0.170.000.00NAN1+0.280.170.061+1+0.320.180.071+1+0.120.150.06NAN1+0.170.080.041+ 1+0.310.160.09NAN1+0.390.120.071+1+0.200.200.161+1+0.220.140.070.1020.0600.041101010Page 474HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-week4Group2M2000mg/m(3)AnimalNumber2051205220532054205520562057205820592060MeanSDnHGBg/dL16.115.716.515.716.115.215.915.515.716.015.80.3610HCT%49.947.350.647.849.746.248.546.748.049.548.41.4710RBCx10A6/uL8.728.689.038.979.028.208.608.028.699.328.730.39210RETICPLTMCVMCHMCHCWBCX10A9/LX10A3/ULfLpgg/dLx10A3/uL167.975157.218.432.213.33168.392254.518.133.114.20178.792156.118.332.616.42174.0112153.317.532.820.54215.369055.117.932.512.08201.9130956.318.633.016.81145.682456.418.532.819.43178.368358.319.333.217.92174.397855.218.032.717.06166.293753.117.132.317.24177.191455.618.232.716.5019.35 194.91.650.610.332.641101010101010Page 475HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-Week4Group2M2000mg/m(3)AnimalNumber2051205220532054205520562057205820592060MeanSDnANEUx10A3/uL2.161.201.171.601.512.381.472.112.831.401.780.55510ALYMXl0A3/uL10.2912.4414.6818.199.9413.4217.3514.9913.4114.8813.962.67410AMONOx10A3/uL0.480.200.270.300.260.710.230.360.430.400.360.15210AEOSABASOALUCPoik PolyX10A3/uLX10A3/ULX10A3/UL0.180.140.09NAN1+0.170.120.071+1+0.090.130.071+1+0.110.230.101+ 1+0.210.090.071+ 1+0.090.110.10NAN1+0.120.190.081+ 1+0.190.200.081+1+0.110.160.121+1+0.220.190.151+ 1+0.15 0.160.090.0500.0450.026101010Page 476HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-Week4Group3M10000mg/m(3)AnimalNumber3051305230533054305530563057305830593060MeanSDnHGBg/dL15.815.616.215.615.015.614.715.715.715.315.50.4210HCT%47.649.448.947.143.648.543.747.748.547.047.22.0210RBCx10A6/uL8.708.968.438.207.868.947.588.688.988.318.460.48110RETICPLTMCVMCHMCHCWBCX10A9/LX10A3/ULfLpgg/dLX10A3/UL188.868154.618.133.220.76213.6105255.117.431.616.34160.9113558.019.333.220.17172.691757.419.033.116.75136.8117855.519.134.59.12152.680354.217.532.217.70182.980957.619.433.620.24147.970155.018.132.815.20139.4121454.017.532.516.50192.765856.518.532.718.85168.891555.818.432.917.1625.48215.01.480.780.793.40610 10 101010 10Page 477HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-Week4Group3M10000mg/m(3)AnimalNumber3051305230533054305530563057305830593060MeanSDnANEUX10A3/uL2.561.462.220.990.781.901.861.551.042.301.670.60510ALYMx10A3/uL17.1014.3816.8015.168.0314.7717.5812.7814.7115.6114.692.74410AMONOAEOSABASOALUCPoikPolyx10A3/uLX10A3/ULx10A3/uLX10A3/UL0.250.500.220.131+1+0.190.050.150.111+ 1+0.680.180.190.111+ 1+0.270.070.150.111+1+0.170.040.050.041+1+0.580.190.170.091+ 1+0.440.110.150.111+1+0.380.210.150.121+1+0.400.060.110.171+1+0.490.240.150.071+ 1+0.390.170.150.110.1680.1390.0450.03510101010Page 478HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-Week4Group4M20000mg/m(3)AnimalNumber4061406240634064406540664067406840694070MeanSDnHGBg/dL15.716.215.315.315.115.815.614.914.415.215.40.5110HCT%47.750.046.346.545.449.146.746.144.247.446.91.7010RBCX10A6/uL8.559.288.318.448.298.918.468.378.468.448.550.30910RETICPLTMCVMCHMCHCWBCx10A9/LX10A3/ULfLpgg/dLX10A3/uL158.7119055.818.332.812.25118.469353.917.532.514.02139.7133555.818.433.015.19169.767555.118.233.018.80146.8102354.818.333.312.16192.673155.117.732.217.07155.162555.218.433.312.41203.269855.117.832.317.13133.2137052.217.032.512.50140.896156.218.032.014.62155.893054.918.032.714.6226.41287.21.150.460.462.392101010101010Page 479HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-Week4Group4M20000mg/m{3)AnimalNumber4061406240634064406540664067406840694070MeanSDnANEU1.783.931.352.301.362.281.412.701.651.392.020.82410ALYM9.969.0413.0015.0210.2814.0310.2413.4610.3412.7311.812.06210AMONO0.330.400.490.690.190.340.160.280.230.230.330.16010AEOSABASOALUCPoikPoly0.050.090.041+1+0.430.150.071+1+0.130.110.091+1+0.440.190.161+1+0.170.100.071+1+0.130.160.121+1+0.450.090.061+1+0.400.150.141+1+0.040.100.141+1+0.090.110.081+1+0.230.130.100.1740.0350.040101010Page 480HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-week4Group1F0mg/m(3)AnimalNumber1561156215631564156515661567156815691570MeanSDnHGBg/dL15.816.515.615.315.216.016.015.415.215.015.60.4710HCT%46.549.946.045.746.147.547.546.145.245.246.61.4210RBCx10-6/uL8.428.928.578.398.238.498.557.958.058.218.380.28210RETICPLTMCVMCHMCHCWBCx10-9/Lx10-3/uLfLpgg/dLx10-3/uL171.9112255.218.834.019.05220.398455.918.533.215.91196.3116153.618.234.019.58174.672854.518.333.517.46170.9120856.018.533.012.84222.089156.018.833.718.62267.884655.518.733.718.45153.491657.919.433.510.23185.4107056.118.833.514.68181.1106955.018.333.210.37194.4100055.618.633.515.7233.69153.01.140.350.333.544101010101010Page 481HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-Week4Group1Fomg/m(3)AnimalNumber1561156215631564156515661567156815691570MeanSDnANEU1.891.551.452.211.362.412.261.321.881.451.780.40710ALYM16.0813.5817.1714.4710.9015.2415.158.3512.188.3813.153.10610AMONOAEOSABASOALUCPoikPoly0.520.130.210.211+ 1+0.410.11 0.180.091+ 1+0.490.18 0.160.141+ 1+0.350.170.140.111+ 1+0.340.070.090.071+ 1+0.350.300.210.111+1+0.460.270.220.091+ 1+0.300.120.080.071+ 1+0.370.100.090.071+ 1+0.320.090.080.071+ 1+0.390.15 0.150.100.0750.0770.0580.04410101010Page 482HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-Week4Group2F2000mg/m(3)AnimalNumber2551255225532554255525562557255825592560MeanSDnHGBg/dL15.615.616.816.216.316.214.514.817.315.615.90.8510HCT%47.845.850.350.447.649.141.844.254.547.447.93.5410RBCRETICX10A6/uLx10A9/L8.54169.08.80143.99.12228.29.21164.38.49160.08.69186.97.20219.18.41181.39.88223.68.76219.88.71189.60.68730.791010PLTMCVMCHMCHCWBCX10A3/uLfLpgg/dLX10A3/uL113656.018.232.515.88119452.117.734.010.9691955.118.533.515.4991154.717.632.217.6993356.119.234.213.9779156.518.633.012.95101858.020.234.813.32128452.617.633.517.8583755.117.531.713.3566854.117.832.917.9096955.018.333.214.94190.61.780.870.962.40110 101010 10Page 483HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-Week4Group2F2000mg/m(3)AnimalNumber2551255225532554255525562557255825592560MeanSDnANEUXl0A3/UL0.770.821.301.531.171.481.662.121.311.821.400.42010ALYMXl0A3/uL14.509.5813.6915.4612.2310.8110.7314.3811.5814.9912.802.06810AMONOxl0A3/uL0.240.260.180.340.270.330.340.340.180.570.310.11210AEOSABASOALUCPoik PolyXl0A3/uLXl0A3/ULXl0A3/UL0.110.130.131+1+0.100.080.121+ 1+0.070.150.101+1+0.100.170.091+1+0.120.100.081+1+0.160.090.091+1+0.420.110.071+1+0.690.180.141+1+0.110.100.071+1+0.240.160.121+1+0.210.130.100.1970.0360.025101010Page 484HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-Week4Group3F10000mg/m(3)AnimalNumber3551355235533554355535563557355835593560MeanSDnHGBg/dL15.714.615.414.715.815.515.714.715.914.415.20.5710HCT%47.244.146.143.048.546.248.244.446.842.045.72.1910RBCRETIC8.63158.77.92217.18.42170.57.36257.88.60181.28.31137.08.66224.88.03162.18.36184.47.50195.38.18188.90.46435.921010PLTMCVMCHMCHCWBCfLpgg/dL118454.718.233.316.81115255.718.433.014.36133654.818.333.312.4985958.419.934.111.9342156.418.432.622.3466155.618.733.619.39107155.718.132.515.2279455.318.333.211.5675956.019.034.014.75127655.919.134.315.2295155.918.633.415.41297.81.040.550.613.3811010101010Page 485HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-Week4Group3F10000mg/m(3)AnimalNumber3551355235533554355535563557355835593560MeanSDnANEUxlOh3/uL1.852.071.691.452.352.961.511.311.321.401.790.53510ALYMxlOh3/uL14.3711.4910.389.9718.7615.1813.079.5712.5313.3512.872.79010AMONOAEOSABASOALUCPoikPolyxlOh3/uLxlOh3/uLxlOh3/uLxlOh3/uL0.230.100.150.111+ 1+0.540.080.090.091+1+0.170.080.090.081+ 1+0.240.140.070.051+ 1+0.290.560.210.171+ 1+0.470.480.180.121+ 1+0.350.110.110.071+ 1+0.340.130.110.101+1+0.290.390.130.091+1+0.200.060.090.121+1+0.310.210.120.100.1180.1880.0450.03310101010Page 486HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-Week4Group4F20000mg/m(3)AnimalNumber4561456245634564456545664567456845694570MeanSDnHGBg/dL13.514.715.315.815.615.214.715.216.015.215.10.7110HCT%39.643.847.547.545.645.743.844.848.445.245.22.5110RBCXl0A6/uL7.088.108.158.628.328.787.648.528.788.738.270.55510RETICPLTMCVMCHMCHCWBCX10A9/LX10A3/uLfLpgg/dLX10A3/uL239.9108655.919.034.010.81205.7114254.018.233.616.32198.173858.318.832.211.76187.2136855.018.333.313.42177.995654.918.734.115.14142.8127352.117.333.215.53195.797757.319.233.617.60113.078952.617.833.916.38221.381555.118.233.010.61169.2133351.817.433.513.93185.1104854.718.333.414.1536.99229.92.150.650.562.460101010101010Page 487HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-Week4Group4F20000mg/m(3)AnimalNumber4561456245634564456545664567456845694570MeanSDnANEU1.311.771.560.821.062.712.392.471.330.841.630.68910ALYM9.1213.959.3712.2813.3912.1614.3613.018.5812.6011.882.09610AMONOAEOSABASOALUCPoikPoly0.190.070.060.051+1+0.210.130.160.091+1+0.240.440.090.071+1+0.080.030.110.101+ 1+0.300.220.110.071+1+0.350.100.120.101+1+0.320.240.130.151+ 1+0.430.280.120.071+1+0.200.350.090.051+1+0.190.070.090.141+1+0.250.190.110.090.1000.1360.0270.03410101010Page 488HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-TerminationGroup1M0mg/m(3)AnimalNumber1061106210631064106510661067106810691070MeanSDnHGBHCTg/dL%16.648.616.648.215.748.816.247.416.448.516.148.215.044.417.050.416.749.716.047.116.248.10.581.631010RBCX10A6/uL9.559.189.069.059.279.298.769.659.378.419.160.36710RETICPLTMCVMCHMCHCWBCx10A9/LX10A3/ULfLpgg/dLX10A3/uL160.875550.917.434.111.83170.485052.518.134.510.56146.678653.917.432.313.68158.990952.417.934.216.16216.472552.317.733.917.22203.639851.917.333.410.94188.0137650.717.133.79.19205.256252.217.733.88.06194.5113753.017.833.511.79247.1105556.019.033.911.40189.285552.617.733.712.0830.69282.91.520.540.602.874101010101010Page 489HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-TerminationGroup1M0mg/m(3)AnimalNumber1061106210631064106510661067106810691070MeanSDnANEUALYMX10A3/uLX10A3/UL2.109.001.918.123.309.583.3312.171.8814.253.076.922.176.571.456.291.939.081.509.422.269.140.7092.5011010AMONOAEOSABASOALUCPoikPolyX10A3/ULXl0A3/uLx10A3/uLx10A3/UL0.350.220.110.041+1+0.260.180.070.02NAN1+0.500.130.110.051+1+0.350.140.120.061+1+0.370.410.200.101+1+0.250.350.320.031+1+0.220.170.040.021+1+0.240.080.000.001+1+0.470.140.080.09NAN1+0.240.110.060.07NAN1+0.330.190.110.050.1000.1070.0910.03210101010Page 490HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-TerminationGroup2M2000mg/m(3)AnimalNumber2051205220532054205520562057205820592060MeanSDnHGBHCTg/dL%16.047.215.244.016.648.416.750.116.548.016.147.616.447.916.849.115.547.5NSNS16.247.80.551.6799RBCRETIC9.01137.38.58185.79.12177.79.99148.59.46160.98.96187.78.97169.29.25195.99.00166.0NSNS9.15169.90.39519.1199PLTMCVMCHMCHCWBCfLpgg/dL117752.417.733.814.97124351.217.834.710.89113653.118.234.414.43111950.216.733.321.4776050.717.434.38.57122053.217.933.710.98118053.418.334.38.03112553.118.234.212.61116952.817.232.517.61NSNSNSNSNS112552.217.733.913.28143.21.210.530.684.34799999Page 491HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-TerminationGroup2M2000mg/m(3)AnimalNumber2051205220532054205520562057205820592060MeanSDnANEUALYM4.1510.181.878.562.3011.671.6618.801.756.411.359.021.056.701.909.812.8313.70NSNS2.1010.540.9253.84499AMONO0.370.260.180.360.140.330.100.480.59NS0.310.1619AEOSABASOALUCPoikPoly0.080.140.061+1+0.090.070.051+1+0.100.120.07NAN1+0.250.200.201+1+0.190.050.03NAN1+0.150.060.061+1+0.110.040.041+1+0.270.100.061+ 1+0.160.190.131+ 1+NSNSNSNSNS0.160.110.080.0690.0590.054999Page 492HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-TerminationGroup3M10000mg/m(3)AnimalNumber3051305230533054305530563057305830593060MeanSDnHGBHCTg/dL%17.048.913.949.015.946.416.649.815.945.816.950.414.541.914.442.715.645.416.147.415.746.81.082.901010RBCRETIC9.50176.49.61203.68.67169.79.23191.58.79141.09.85169.87.82182.18.12464.29.33143.28.80178.08.97202.00.65594.131010PLTMCVMCHMCHCWBCfLpgg/dL124251.517.934.713.1794051.014.528.412.28130653.518.434.312.4895353.918.033.312.48136652.118.034.76.9683851.217.233.611.9191353.518.534.612.61119352.717.733.68.74135348.616.834.59.58112953.918.334.110.80112352.217.533.611.10197.81.681.191.892.0421010101010Page 493HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-TerminationGroup3M10000mg/m(3)AnimalNumber3051305230533054305530563057305830593060MeanSDnANEUALYMxl0A3/uLXl0A3/uL1.9210.732.059.812.019.571.5010.441.575.061.789.703.038.641.386.980.868.301.558.821.778.810.5661.7131010AMONOXl0A3/UL0.160.170.420.290.190.210.730.150.210.210.270.17910AEOSABASOALUCPoik PolyXl0A3/uLXl0A3/uLXl0A3/uL0.230.090.041+1+0.110.080.07NAN1+0.370.090.04NAN1+0.100.090.06NAN1+0.080.030.021+1+0.100.080.05NAN1+0.100.070.041+1+0.120.060.041+1+0.100.050.051+ 1+0.100.070.05NAN1+0.140.070.050.0910.0200.013101010Page 494HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-TerminationGroup4M20000mg/m(3)AnimalNumber4061406240634064406540664067406840694070MeanSDnHGBHCTg/dL%16.548.717.552.516.549.816.749.315.244.415.848.415.746.215.748.014.842.915.746.916.047.70.792.761010RBC9.3810.239.519.498.519.428.899.278.799.099.260.47710RETICPLTMCVMCHMCHCWBCfLpgg/dL132.988951.917.734.011.23160.562251.417.133.410.30161.1124852.417.333.013.81119.4101651.917.633.918.49146.380152.217.934.38.72184.293451.416.732.611.68143.4130952.017.734.110.01167.8103551.816.932.610.81148.4138648.816.934.67.90136.8119651.617.333.610.15150.1104451.517.333.611.3118.81241.81.010.410.702.992101010101010Page 495HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-TerminationGroup4M20000mg/m(3)AnimalNumber4061406240634064406540664067406840694070MeanSDnANEUALYM1.628.813.137.352.0910.991.6315.551.187.181.549.361.528.081.918.301.456.111.947.761.808.950.5372.6721010AMONOAEOSABASOALUCPoikPoly0.270.400.090.031+ 1+0.110.220.000.00NAN1+0.350.210.100.07NAN1+0.700.260.200.15NAN1+0.170.110.060.031+ 1+0.240.390.080.061+1+0.180.100.060.071+1+0.340.110.090.06NAN1+0.170.080.040.05NAN1+0.250.080.060.06NAN1+0.280.200.080.060.1670.1220.0520.03910101010Page 496HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-TerminationGroup1F0mg/m(3)AnimalNumber1561156215631564156515661567156815691570MeanSDnHGBHCTg/dL%16.146.715.444.515.646.415.645.615.244.515.544.515.946.315.344.716.448.214.843.415.645.50.461.421010RBCRETIC8.29140.58.28212.88.86139.78.54161.18.13151.68.08216.38.37201.18.03143.28.66178.28.08168.28.33171.30.27829.671010PLTMCVMCHMCHCWBCfLpgg/dL80456.419.434.411.68139353.718.634.611.62102152.417.633.711.1995553.418.234.28.69129554.818.634.07.6590655.019.134.813.1683055.319.034.313.5872555.619.034.24.44124355.718.933.911.60106953.718.234.05.39102454.618.734.29.90224.51.250.540.333.1891010101010Page 497HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-TerminationGroup1F0mg/m(3)AnimalNumber1561156215631564156515661567156815691570MeanSDnANEUALYM1.899.264.306.741.808.781.007.291.715.622.909.362.2210.721.153.200.8110.220.984.171.887.541.0702.5751010AMONOAEOSABASOALUCPoikPoly0.220.180.070.061+ 1+0.390.100.040.051+1+0.250.260.070.041+1+0.190.120.050.041+ 1+0.170.070.050.032+1+0.350.380.100.09NAN1+0.380.140.070.05NAN1+0.040.040.000.001+1+0.290.160.060.06NAN1+0.100.090.030.02NAN1+0.240.150.050.040.1170.1010.0270.02510101010Page 498HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-TerminationGroup2F2000mg/m(3)AnimalNumber2551255225532554255525562557255825592560MeanSDnHGBHCTg/dL%16.047.216.547.816.448.316.449.416.746.916.848.114.240.815.244.017.453.615.746.116.147.20.913.341010RBC8.609.318.939.038.468.587.188.469.688.668.690.66210RETICPLTMCVMCHMCHCWBCfLpgg/dL154.190355.018.633.913.13150.691851.417.734.46.91181.695554.118.434.08.94176.497154.618.233.310.80155.080055.419.835.78.73189.371256.019.534.98.57178.7111056.819.734.76.16152.898952.018.034.69.14220.967855.318.032.510.37157.8117353.218.234.112.81171.792154.418.634.29.5622.32158.21.730.770.882.268101010101010Page 499HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-TerminationGroup2F2000mg/m(3)AnimalNumber2551255225532554255525562557255825592560MeanSDnANEUALYMX10A3/ULX10A3/uL1.4911.111.015.560.737.911.119.240.767.581.077.040.875.050.887.861.668.500.9711.101.068.100.3032.0211010AMONOAEOSABASOALUCPoikPolyX10A3/ULX10A3/ULx10A3/uLX10A3/uL0.220.160.090.07NAN1+0.120.160.040.031+1+0.160.050.050.04NAN1+0.180.160.070.05NAN1+0.150.140.050.04NAN1+0.200.180.050.04NAN1+0.140.050.030.02NAN1+0.150.140.060.051+1+0.100.100.000.001+ 1+0.380.180.070.121+ 1+0.180.130.050.050.0790.0490.0250.03210101010Page 500HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-TerminationGroup3F10000mg/m(3)AnimalNumber3551355235533554355535563557355835593560MeanSDnHGBHCTg/dL%15.845.416.549.016.046.114.742.916.146.216.447.615.946.615.544.116.246.016.145.615.946.00.521.691010RBCx10A6/uL8.538.968.497.408.468.678.418.058.458.508.390.41510RETICPLTMCVMCHMCHCWBCx10A9/LX10A3/uLfLpgg/dLx10A3/uL137.2100053.318.534.711.88234.9107654.718.433.611.41146.8141954.318.834.66.62209.9100658.019.834.27.61205.068654.619.034.916.29167.983254.918.934.59.15205.993555.418.934.09.21174.780354.819.335.27.86133.6100554.519.235.28.37180.5136853.618.935.310.38179.6101354.819.034.69.8834.10232.11.280.400.562.805101010101010Page 501HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-TerminationGroup3F10000mg/m(3)AnimalNumber3551355235533554355535563557355835593560MeanSDnANEUALYMX10A3/ULX10A3/UL1.4410.112.567.850.605.751.226.012.5512.561.846.651.187.631.126.381.166.721.788.171.557.780.6372.1101010AMONOAEOSABASOALUCPoikPolyX10A3/ULX10A3/uLX10A3/ULX10A3/uL0.100.100.080.051+ 1+0.490.340.100.061+1+0.070.090.060.04NAN1+0.260.050.030.031+1+0.260.710.140.07NAN1+0.200.340.060.06NAN1+0.190.120.040.051+1+0.170.090.050.051+ 1+0.160.250.040.04NAN1+0.150.130.060.071+1+0.210.220.070.050.1170.2010.0330.01310101010Page 502HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-TerminationGroup4F20000mg/m(3)AnimalNumber4561456245634564456545664567456845694570MeanSDnHGBHCTg/dL%15.044.215.845.316.248.516.548.216.348.316.147.9cscs15.044.215.946.615.445.115.846.50.551.8199RBCRETIC8.04212.68.41208.48.33232.49.01147.18.54196.19.42127.1cs cs8.55194.78.38216.18.83205.58.61193.30.41434.1499PLTMCVMCHMCHCWBCfLpgg/dL119454.918.734.09.2293053.818.834.910.5662558.219.533.510.81113753.518.334.26.5672456.519.133.78.21107650.817.133.78.80cs cs cs cscs101751.717.533.911.70103455.619.034.26.80143951.117.534.210.45102054.018.434.09.23243.62.540.840.411.80799999Page 503HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-TerminationGroup4F20000mg/m(3)AnimalNumber4561456245634564456545664567456845694570MeanSDnANEUALYM1.627.261.348.802.067.930.555.791.076.690.627.80cscs1.389.731.225.242.167.891.347.460.5591..40699AMONO0.110.170.100.060.190.17cs0.220.200.180.160.0539AEOSABASOALUCPoikPoly0.100.090.051+1+0.12 0.08 0.06NAN1+0.210.000.00NAN1+0.070.06 0.021+1+0.190.050.03NAN1+0.120.05 0.041+1+cscscs cscs0.250.070.061+ 1+0.14 0.00 0.00NAN1+0.100.040.08NAN1+0.14 0.05 0.040.059 0.0320.028999Page 504HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-RecoveryGroup1M0mg/m(3)AnimalNumber1051105210531054105510561057105810591060MeanSDnHGBg/dL14.815.715.616.116.015.515.914.516.115.215.50.5510HCT%46.348.449.349.544.047.948.745.651.749.448.12.2310RBC8.739.069.309.238.268.949.248.799.709.729.100.44610RETICPLTMCV MCHMCHCWBCfLpgg/dL198.9103553.017.032.016.84139.0111453.517.332.413.24133.1123253.016.831.710.74210.081153.617.432.516.10105.589853.219.336.39.13151.882053.517.332.412.81145.186752.717.232.66.88186.3128251.916.531.813.09202.168653.416.631.216.91152.3108350.815.630.810.48162.498352.917.132.412.6234.81196.20.880.941.503.370101010101010Page 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506HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-RecoveryGroup4M20000mg/m(3)AnimalNumber4051405240534054405540564057405840594060MeanSDnHGBg/dL14.915.715.915.416.016.115.714.515.015.615.50.5210HCT%45.547.948.848.444.949.944.347.146.748.547.21.8410RBCX10A6/UL9.059.309.199.328.129.138.379.378.999.038.990.41610RETICPLTMCVMCHMCHCWBCX10A9/LX10A3/ULfLpgg/dLx10A3/uL128.5129850.316.532.715.74127.0130851.516.932.712.76131.785353.117.332.614.81138.2115952.016.631.99.60133.2102655.219.835.88.11104.2102754.717.632.213.95124.381052.918.835.59.23127.9125850.315.530.911.12108.659951.916.732.18.44144.885753.717.332.216.47126.8102052.617.332.912.0212.34238.51.681.221.563.131101010101010Page 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508HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixIIndividualHematologyValues-RecoveryGroup1F0mg/m(3)AnimalNumber1551155215531554155515561557155815591560MeanSDnHGBg/dL14.215.916.415.315.715.614.615.015.614.815.30.6610HCT%42.046.750.146.545.946.442.745.547.044.945.82.2710RBCRETICX10A6/ULX10A9/L7.90133.98.34167.48.91132.08.25115.98.18143.58.33114.87.72157.38.24136.18.28152.58.4176.58.26133.00.31426.021010PLTMCVMCHMCHCWBCX10A3/uLfLpgg/dLX10A3/uL125053.117.933.811.30130155.919.034.010.8697056.318.432.78.2986656.318.532.812.3484756.119.234.310.20109555.718.733.510.0278055.318.934.111.01122355.218.233.08.38101756.718.833.211.94109253.417.633.111.85104455.418.533.510.62180.11.220.510.571.4091010101010Page 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# Huntingdon Life Sciences 00

- 6130 Page 513

211

- DIPE

- S Final Report

Individual Coagulation Values

Preface Appendix J

Abbreviation Parameter Reporting Units

PT Prothrombin Time seconds

APTT Activated Partial Thromboplastin Time seconds

Key to Other Abbreviations

NS = No specimen

QNS = Quantity not sufficient to complete request.HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixJIndividualCoagulationValues-Week4Group1M0mg/m(3)AnimalNumber1061106210631064106510661067106810691070MeanSDnPTSeconds12.411.712.211.412.210.911.512.512.011.711.90.5010APTTSeconds19.717.218.515.219.913.514.821.819.616.317.72.6710Page 514HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixJIndividualCoagulationValues-Week4Group2M2000mg/m(3)AnimalNumber2051205220532054205520562057205820592060MeanSDnPTSeconds13.012.512.412.712.812.212.611.812.712.612.50.3410APTTSeconds21.119.519.021.120.217.921.819.422.921.420.41.5010Page 515HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixJIndividualCoagulationValues-Week4Group3M10000mg/m(3)AnimalNumber3051305230533054305530563057305830593060MeanSDnPTSeconds12.312.312.611.913.011.911.913.912.813.012.60.6410APTTSeconds18.520.821.719.820.218.518.323.122.520.920.41.6910Page 516HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixJIndividualCoagulationValues-Week4Group4M20000mg/m(3)AnimalNumber4061406240634064406540664067406840694070MeanSDnPTSeconds13.112.312.112.311.512.613.511.811.713.612.50.7410APTTSeconds21.720.621.019.419.023.625.318.720.921.221.12.0410Page 517HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixJIndividualCoagulationValues-Week4Group1F0mg/m(3)AnimalNumber1561156215631564156515661567156815691570MeanSDnPTSeconds11.111.511.112.311.211.111.211.512.010.811.40.4610APTTSeconds21.420.016.620.219.220.219.420.020.520.219.81.2610Page 518HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixJIndividualCoagulationValues-Week4Group2F2000mg/m(3)AnimalNumber2551255225532554255525562557255825592560MeanSDnPTSeconds12.111.512.211.510.312.111.4QNS11.311.511.50.589APTTSeconds21.118.821.318.917.420.916.5QNS20.118.219.21.719Page 519HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixJIndividualCoagulationValues-Week4Group3F10000mg/m(3)AnimalNumber3551355235533554355535563557355835593560MeanSDnPTSecondsll.511.112.0ll.511.7ll.412.011.612.011.2ll.60.3310APTTSeconds19.518.921.720.018.718.822.119.822.313.119.52.6310Page 520HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixJIndividualCoagulationValues-Week4Group4F20000mg/m(3)AnimalNumber4561456245634564456545664567456845694570MeanSDnPTSeconds12.211.111.411.212.112.211.511.510.911.411.60.4610APTTSeconds21.018.719.220.921.521.018.719.218.918.719.81.1610Page 521HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixJIndividualCoagulationValues-TerminationGroup1M0mg/m(3)AnimalNumber1061106210631064106510661067106810691070MeanSDnPTAPTTsecondsSeconds12.221.112.217.811.915.911.822.211.8 13.912.022.211.817.012.618.513.223.912.2 19.212.2 19.20.443.1610 10Page 522HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixJIndividualCoagulationValues-TerminationGroup2M2000mg/m(3)AnimalNumber2051205220532054205520562057205820592060MeanSDnPTAPTTSecondsSeconds11.817.712.023.413.022.112.822.412.6 18.212.420.813.324.512.619.913.621.7NSNS12.721.20.582.2799Page 523HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixJIndividualCoagulationValues-TerminationGroup3M10000mg/m(3)AnimalNumber3051305230533054305530563057305830593060MeanSDnPTAPTTSecondsSeconds12.119.111.8 17.914.018.011.617.511.5 15.512.823.012.8 19.212.420.314.825.912.921.712.719.81.063.031010Page 524HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixJIndividualCoagulationValues-TerminationGroup4M20000mg/m(3)AnimalNumber4061406240634064406540664067406840694070MeanSDnPTAPTTSeconds Seconds14.420.912.2 18.812.018.212.717.211.820.912.618.512.425.912.220.012.423.912.320.412.520.50.722.671010Page 525HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixJIndividualCoagulationValues-TerminationGroup1F0mg/m(3)AnimalNumber1561156215631564156515661567156815691570MeanSDnPTAPTTSecondsSeconds11.320.610.618.111.2 14.611.4 17.811.219.211.523.711.117.711.114.211.620.511.2 15.211.218.20.272.991010Page 526HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixJIndividualCoagulationValues-TerminationGroup2F2000mg/m(3)AnimalNumber2551255225532554255525562557255825592560MeanSDnPTAPTTSecondsSeconds11.218.110.917.110.717.511.2 16.210.916.411.918.010.614.811.115.411.414.011.118.011.116.60.371.451010Page 527HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixJIndividualCoagulationValues-TerminationGroup3F10000mg/m(3)AnimalNumber3551355235533554355535563557355835593560MeanSDnPTAPTTSecondsSeconds11.418.111.216.011.320.110.719.411.619.111.115.711.018.411.1 13.911.7 17.511.214.011.2 17.20.292.211010Page 528HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixJIndividualCoagulationValues-TerminationGroup4F20000mg/m(3)AnimalNumber4561456245634564456545664567456845694570MeanSDnPTAPTTSecondsSeconds11.518.111.216.310.516.110.917.611.6 19.911.416.911.318.611.2 16.510.713.611.615.311.2 16.90.381.781010Page 529

# Huntingdon Life Sciences 00

- 6130 Page 530

211

- DIPE -S Final Report

Individual Clinical Chemistry Values

Preface App endix K

Reporting

Abbreviation Parameter Units

AST Aspartate Aminotransferase U/L

ALT Alanine Aminotransferase U/L

ALKP Alkaline Phosphatase U/L

LD Lactate Dehydrogenase U/L

SDH Sorbitol Dehydrogenase U/L

BU N Blood Urea Nitrogen mg/dL

CREAT Creatinine mg/dL

GLU Fasting Glucose mg/dL

CK Creatine Kinase U/L

CHOL Cholesterol (Enzymatic) mg/dL

TP Total Protein g/dL

ALB Albumin g/dL

Glob Globulin (calculated) g/dL

A/G Albumin/Globulin Ratio (calculated)

TBILI Tot al Bilirubin mg/dL

DBILI Direct Bilirubin mg/dL

IBILI Indirect Bilirubin (calculated) mg/dL

Na + 

# Sodium mEq/L

K + 

# Potassium mEq/L

Cl - 

# Chloride mEq/L

Ca ++ 

# Calcium mg/dL

PHOS Inorganic Phosphorus mg/dL

GGT Gamma

- Glutamyl Transferase U/L

Bile Acids Bile Acids μ mol/L

Key to Other Abbreviations

NS

= No specimen

QNS

= Quantity not sufficient to complete request.HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4Group1Momg/m(3)AnimalNumber1061106210631064106510661067106810691070MeanSDnASTALTU/LU/L865681427649613477478845833797497441853681449.76.91010ALKPU/L15114019911719613415421711015915835.810LDSDHBUNCREATGLUCKU/LU/Lmg/dLmg/dLmg/dLU/L21720.214.20.412823317622.69.40.312316620522.413.80.311120417215.311.70.3124 15521417.313.10.311821516017.8 12.10.3138 16817018.1 17.20.4135 15317815.814.70.3141 18912816.411.80.412713823214.1 12.90.312777918518.013.10.312724031.42.902.090.059.1191.8101010101010Page 531HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4Group1M0mg/m(3)AnimalNumber1061106210631064106510661067106810691070MeanSDnCHOLTPmg/dLg/dL746.4636.7647.0736.6736.6786.6636.2526.1526.2716.6666.59.10.271010ALBg/dL4.24.24.44.34.24.04.03.74.14.34.10.2010GlobA/GTBILIDBILIIBILINa+g/dLmg/dL mg/dLmgjdLmEq/L2.21.90.140.040.101482.51.70.110.030.081492.61.70.150.050.101482.31.90.110.040.071462.41.80.170.040.131472.61.50.100.030.071472.21.80.100.030.071492.41.50.140.030.111482.12.00.130.020.111472.31.90.100.040.061472.41.80.130.040.091480.170.170.0250.0080.0231.0101010101010Page 532HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4AnimalGroupNumberK+Cl-Ca++PHOSGGTBileAcidsmEq/L mEq/Lmg/dLmg/dLU/Lumol/L1M10614.810211.29.2096.90mg/m(3)10625.810311.59.7015.610635.210111.79.8081.110646.010211.89.8017.510655.610211.49.7025.010665.410312.011.1017.210675.810311.410.9090.310685.810311.412.02125.010695.410311.48.6013.310706.110311.69.6042.7Mean5.610311.510.0052.5SD0.400.70.241.000.641.76n101010101010Page 533HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4AnimalGroupNumberASTALTALKPLDSDHBUNCREATGLUCKU/LU/LU/LU/LU/Lmg/dL mg/dLmg/dLU/L2M20517936190 12613.112.50.31181642000mg/m(3)2052733214114512.1 11.70.31041712053743817810614.113.40.311912520547846142 11413.4 10.90.31201972055713715711317.114.00.3128 1272056724214713221.915.40.3126 11620577447158 14115.114.40.311214020587232121 17015.511.00.41261832059784916917116.4 10.20.31051662060743518010414.310.40.3125125Mean753915813215.3 12.40.3118151SD2.86.221.324.52.771.830.038.728.3n101010101010101010Page 534HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4AnimalGroupNumberCHOLTPALBGlobA/GTBILIDBILIIBILINa+mg/dLg/dLg/dLg/dLmg/dLmg/dLmg/dLmEq/L2M2051536.54.12.41.70.110.030.081502000mg/m(3)2052406.24.02.21.80.090.010.081472053616.04.02.02.00.110.030.081472054596.54.12.41.70.090.020.071462055616.34.22.12.00.150.030.121472056556.54.12.41.70.130.040.091482057786.44.12.31.80.100.030.071492058776.34.12.21.90.150.040.111472059676.64.22.41.80.130.040.091482060606.74.12.61.60.110.030.08146Mean616.44.12.31.80.120.030.09148SD11.20.210.070.180.130.0220.0090.0161.3n101010101010101010Page 535HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4AnimalGroupNumberK+Cl-Ca++PHOSGGTBileAcidsmEq/LmEq/Lmg/dLmg/dLU/Lumol/L2M20515.610311.810.1045.72000mg/m(3)20525.710211.19.3034.620535.210111.09.7032.220545.810111.39.7011.420555.010311.39.30121.820565.910211.3 10.30302.820575.410411.510.4061.620586.010111.210.3026.920595.310111.59.5072.920605.310111.49.8057.1Mean5.510211.39.8076.7SD0.331.10.230.410.085.14n101010101010Page 536HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4AnimalGroupNumberASTALTALKPLDSDHBUNCREATGLUCKU/LU/LU/LU/LU/Lmg/dL mg/dLmg/dLU/L3M3051894711814120.711.10.311113810000mg/m(3)3052633416514612.612.20.3121 13430531055720227221.811.20.4106323305471311336516.315.40.412211630557534156 13813.511.40.310012930567437156 13613.311.10.3100122305776351187610.78.10.2109144305872391555413.011.00.3112115305975421867712.1 11.70.3116 128306084421687312.512.80.3110139Mean784015611814.7 11.60.3111149SD11.77.727.265.13.761.810.067.662.0n101010101010101010Page 537HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4Group3M10000mg/m(3)AnimalNumber3051305230533054305530563057305830593060MeanSDnCHOLTPmg/dLg/dL486.6696.7416.8476.5496.2566.3616.1626.6716.8665.8576.410.30.331010ALBg/dL4.14.14.44.24.04.04.04.34.33.94.10.1610GlobA/GTBILIDBILIIBILINa+g/dLmg/dLmg/dLmg/dLmEq/L2.51.60.090.020.071482.61.60.130.030.101482.41.80.150.040.111472.31.80.160.040.121482.21.80.090.040.051462.31.70.150.040.11QNS2.11.90.110.030.081482.31.90.130.040.091492.51.70.110.040.071461.92.10.130.040.091462.31.80.130.040.091470.210.150.0250.0070.0221.110101010109Page 538HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4AnimalGroupNumberK+Cl-Ca++PHOSGGTBileAcidsmEq/LmEq/Lmg/dLmg/dLU/Lumol/L3M30516.110511.510.5013.610000mg/m(3)30525.310311.69.6063.330535.610511.5 11.4042.730545.710111.810.6043.130555.010311.19.90221.43056QNSQNS QNS QNS0QNS30575.110211.39.8012.630585.010311.69.3018.130595.010211.69.4019.730605.410311.210.90151.1Mean5.410311.510.2065.1SD0.381.30.220.730.072.88n9999109Page 539HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4AnimalGroupNumberASTALTALKPLDSDHBUNCREATGLUCKU/LU/LU/LU/LU/Lmg/dLmg/dLmg/dLU/L4M406176371747218.39.10.3118 10720000mg/m(3)406268391199416.914.20.3122101406369361737912.412.90.3107101406489491439818.511.50.3971774065634315813911.512.00.3113141406665381549215.1 13.30.3122131406757291247710.413.60.311810340687334106 10213.9 13.10.3114101406977381346913.012.40.3113143407071391507214.411.70.4114 178Mean71381448914.4 12.40.3114128SD8.85.322.721.12.781.440.037.530.8n10 10101010 10 101010Page 540HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4AnimalGroupNumberCHOLTPALBGlobA/GTBILIDBILIIBILINa+mg/dLg/dLg/dLg/dLmg/dLmg/dL mg/dLmEq/L4M4061466.94.42.51.80.140.030.1114720000mg/m(3)4062536.43.82.61.50.090.030.061474063546.24.02.21.80.110.030.081484064586.24.22.02.10.110.030.081494065596.03.82.21.70.090.020.071474066696.54.22.31.80.110.040.071494067676.84.42.41.80.110.030.081484068776.13.82.31.70.120.020.101464069496.34.12.21.90.110.050.061484070576.23.92.31.70.080.030.05150Mean596.44.12.31.80.110.030.08148SD9.60.300.240.170.150.0170.0090.0181.2n101010101010101010Page 541HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4AnimalGroupNumberK+Cl-Ca++PHOSGGTBileAcidsmEq/L mEq/Lmg/dL mg/dLU/Lumol/L4M40614.910111.69.1010.420000mg/m(3)40626.010411.310.7019.140635.59911.510.4055.940645.910411.69.5022.540655.810511.69.9028.040664.810211.59.6033.140675.010211.48.6033.740685.510511.310.2075.040695.310210.89.3083.040706.310510.710.4014.1Mean5.510311.39.8037.5SD0.502.00.330.670.025.35n101010101010Page 542HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4AnimalGroupNumberASTALTALKPLDSDHBUNCREATGLUCKU/LU/LU/LU/LU/Lmg/dLmg/dL mg/dLU/L1F15617948976816.6 12.70.4126131omg/m(3)15627731856817.3 13.80.61281461563793410613414.615.20.4126103156493401169316.516.20.5114 132156583381017415.7 15.90.5119 1181566104489417418.615.20.511727115676429976616.214.40.512498156866251054513.214.10.41238615695730867312.514.80.41168515706641647011.513.40.5123106Mean7736958715.3 14.60.5122 128SD14.37.914.338.62.261.100.074.854.3n101010101010101010Page 543HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4AnimalGroupNumberCHOLTPALBGlobA/GTBILIDBILIIBILINa+mg/dLg/dL g/dL g/dLmg/dLmg/dLmg/dLmEq/L1F1561917.24.82.42.00.130.050.081470mg/m(3)1562957.95.12.81.80.210.060.151501563736.74.22.51.70.190.030.161481564996.84.42.41.80.210.060.151471565697.44.82.61.80.180.040.141471566737.85.02.81.80.210.050.161471567758.05.22.81.90.190.040.151471568967.54.82.71.80.170.040.131491569706.64.42.22.00.150.040.111481570688.35.62.72.10.130.030.10148Mean817.44.82.61.90.180.040.13148SD12.70.590.420.210.130.0310.0110.0281.0n101010101010101010Page 544HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4AnimalGroupNumberK+Cl-Ca++PHOSGGTBileAcidsmEq/LmEq/Lmg/dLmg/dLU/Lumol/L1F15614.810411.710.3052.50mg/m(3)15626.010012.410.6092.115635.710111.510.40141.415645.510011.79.40191.715655.810012.010.30>200.15665.010212.09.90104.815675.210312.210.1028.515684.810212.29.90103.615694.410311.19.1026.415705.610212.69.0015.5Mean5.310211.99.9084.1SD0.521.40.450.560.059.01n10101010109Page 545HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4Group2F2000mg/m(3)AnimalNumber2551255225532554255525562557255825592560MeanSDnASTALTU/LU/L63287427724086366728683097391005064268834783413.87.61010ALKPLDU/LU/L8867101727068135641091081225013821910132795 921259910811721.788.01010SDHBUNCREATGLUCKU/Lmg/dLmg/dLmg/dLU/L10.914.30.411510412.610.90.311814319.212.90.411511712.315.90.512317511.914.90.41261228.714.60.41138328.019.40.520118418.5 12.60.516429812.6 13.50.414919621.412.90.612522315.6 14.20.41351655.972.310.0828.564.61010101010Page 546HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4AnimalGroupNumberCHOLTPALBGlobA/GTBILIDBILIIBILINa+mg/dLg/dLg/dLg/dLmg/dLmg/dLmg/dLmEq/L2F2551767.44.92.52.00.150.020.131482000mg/m(3)2552807.04.52.51.80.150.030.1215025531047.95.12.81.80.200.050.151492554757.54.92.61.90.200.050.151482555716.84.52.32.00.150.030.121472556877.14.42.71.60.130.030.101512557996.74.62.12.20.250.060.191422558QNS7.14.42.71.60.120.020.101522559QNS7.34.82.51.90.140.030.111532560877.55.12.42.10.160.030.13153Mean857.24.72.51.90.170.040.13149SD11.80.360.270.210.200.0400.0140.0273.3n81010101010101010Page 547HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4AnimalGroupNumberK+Cl-Ca++PHOSGGTBileAcidsmEq/L mEq/Lmg/dL mg/dLU/Lumol/L2F25516.310311.910.1015.02000mg/m(3)25525.010411.79.7020.125536.210112.28.8019.225545.310212.210.4175.025555.510311.610.3018.425565.810312.012.0017.625576.19912.015.10>200.25586.611011.611.3021.525596.210812.210.4018.225606.610712.110.2033.0Mean6.010412.010.8026.4SD0.543.40.241.730.318.91n10101010109Page 548HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4Group3F10000mg/m(3)AnimalNumber3551355235533554355535563557355835593560MeanSDnASTALTU/LU/L622870377132713575386937632865356928843370336.34.01010ALKPU/L8012368108779280781191689930.710LDSDHBUNCREATGLUCKU/LU/Lmg/dL mg/dLmg/dLU/L7711.213.60.31291104210.313.20.4114726510.111.90.41081157716.013.30.5120887210.310.30.4119125479.214.60.4117 1118511.711.10.4109875210.011.60.4114986611.6 11.40.51141319514.6 16.40.51211436811.512.70.411710816.92.171.840.066.222.0101010101010Page 549HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4AnimalGroupNumberCHOLTPALBGlobA/GTBILIDBILIIBILINa+mg/dLg/dLg/dLg/dLmg/dLmg/dLmg/dLmEq/L3F3551797.04.72.32.00.140.010.1315310000mg/m(3}35521077.24.52.71.70.200.050.1515235531017.65.02.61.90.180.040.141503554947.95.22.71.90.170.040.131503555836.74.52.22.00.150.030.121533556QNS7.55.02.52.00.190.040.151523557QNS7.44.82.61.80.140.030.111523558987.34.72.61.80.130.020.1115235591157.85.02.81.80.240.060.181513560857.44.72.71.70.170.060.11151Mean957.44.82.61.90.170.040.13152SD12.50.360.230.190.120.0330.0160.0231.1n81010101010101010Page 550HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4AnimalGroupNumberK+Cl-Ca++PHOSGGTBileAcidsmEq/LmEq/Lmg/dLmg/dLU/Lumol/L3F35516.210811.9 11.7016.010000mg/m(3)35525.310311.910.40>200.35536.010311.89.2024.435545.810512.09.6034.835555.510811.79.6017.135565.810412.39.8059.735576.110512.110.2015.735585.910512.310.2026.735595.710412.210.00166.435605.310512.011.70>200.Mean5.810512.010.2045.1SD0.311.80.200.850.051.12n10101010108Page 551HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4AnimalGroupNumberASTALTALKPLDSDHBUNCREATGLUCKU/LU/LU/LU/LU/Lmg/dLmg/dL mg/dLU/L4F4561673274399.012.80.41218220000mg/m(3)45627127716011.615.30.411211745636931684510.115.30.4136137456473301059916.011.20.4106104456562311004310.312.40.41167345666431973714.914.30.41179845676832797811.710.40.41221524568752098 9212.715.70.5107275456970271097715.2 13.40.4113 1244570673086458.014.90.411489Mean6929896212.013.60.4116125SD3.93.715.023.22.731.840.038.658.2n101010101010101010Page 552HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4Group4F20000mg/m(3)AnimalNumber4561456245634564456545664567456845694570MeanSDnCHOLTPmg/dLg/dL696.7877.4697.81027.31117.0907.6QNS7.4836.61218.2QNS7.0927.318.80.49810ALBg/dL4.44.85.24.64.65.04.94.45.24.84.80.2910GlobA/GTBILIDBILIIBILINa+g/dLmg/dL mg/dLmg/dLmEq/L2.31.90.070.030.041522.61.80.140.030.111512.62.00.180.030.151542.71.70.100.020.081532.41.90.180.030.151532.61.90.160.050.111502.52.00.160.020.141542.22.00.170.050.121513.01.70.130.030.101502.22.20.170.040.131542.51.90.150.030.111520.250.150.0370.0110.0341.6101010101010Page 553HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-Week4AnimalGroupNumberK+Cl-Ca++PHOSGGTBileAcidsmEq/LmEq/Lmg/dLmg/dLU/Lumol/L4F45615.410611.210.1017.720000mg/m(3)45625.910811.68.0031.445635.510612.410.2080.745646.110511.89.9034.845655.710411.99.4039.145665.110211.88.7042.745675.910811.99.3017.545685.010711.610.20>200.45696.010512.29.6019.745705.610611.79.7024.5Mean5.610611.89.5034.2SD0.371.80.330.710.019.76n10101010109Page 554HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-TerminationGroup1Momg/m(3)AnimalNumber1061106210631064106510661067106810691070MeanSDnASTU/L8780867979907811484738511.410ALTALKPU/LU/L55884397549241117438242693477461054672399944906.415.210 10LDU/L11613012579140159225496186153181117.610SDHBUNCREATGLUCKU/Lmg/dLmg/dLmg/dLU/L17.1 12.80.413725715.813.60.41469412.5 13.00.519518913.215.60.41517614.118.50.517811323.313.70.412726415.016.60.4128 10425.817.50.514347317.7 16.70.517124121.216.00.31178517.615.40.41491904.482.010.0724.9124.410 1010 1010Page 555HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-TerminationGroup1M0mg/m(3)AnimalNumber1061106210631064106510661067106810691070MeanSDnCHOLmg/dL874863778582635950776914.310TPALBg/dL g/dL7.24.37.14.37.34.37.14.47.74.67.34.26.64.06.53.86.94.27.14.57.14.30.350.231010GlobA/GTBILIDBILIIBILINa+g/dLmg/dL mg/dLmg/dLmEq/L2.91.50.120.050.071492.81.50.110.040.071483.01.40.120.040.081492.71.60.110.030.081503.11.50.130.060.071483.11.40.100.030.071492.61.50.100.010.091472.71.40.100.010.091472.71.60.080.010.071472.61.70.140.010.131472.81.50.110.030.081480.190.100.0170.0190.0191.1101010101010Page 556HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-TerminationAnimalGroupNumberK+Cl-Ca++PROSGGTBileAcidsmEq/L mEq/Lmg/dLmg/dLU/Lumol/L1M10615.710111.78.1038.10mg/m(3)10625.410311.38.4024.810635.410311.810.0068.010645.610411.67.7023.510656.310012.69.2038.710665.89911.18.5013.510676.29810.58.0012.910685.89710.59.2016.310696.39611.48.2013.610706.39810.86.5022.9Mean5.910011.38.4027.2SD0.372.80.650.960.017.16n101010101010Page 557HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-TerminationGroup2M2000mg/m(3)AnimalNumber2051205220532054205520562057205820592060MeanSDnASTU/L96778791666875100107NS8514.59ALTALKPU/LU/L4267398746995211537874080509939735696NSNS45896.714.899LDU/L5302941451077919399361402NS246159.09SDHBUNCREATGLUCKU/Lmg/dLmg/dLmg/dLU/L13.313.20.413321411.5 14.70.410313620.616.70.620513913.814.40.523512615.115.00.42129417.316.60.412240314.215.80.41279118.513.20.512425428.615.10.5224652NSNSNSNSNS17.015.00.51652345.191.280.0752.5185.299999Page 558HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-TerminationGroup2M2000mg/m(3)AnimalNumber2051205220532054205520562057205820592060MeanSDnCHOLTPmg/dLg/dL676.9336.7506.6637.4666.8597.1857.2907.4616.8NSNS647.017.00.3099ALBg/dL4.04.04.34.64.34.24.34.44.2NS4.30.199GlobA/GTBILIDBILIIBILINa+g/dLmg/dLmg/dLmg/dLmEq/L2.91.40.150.040.111482.71.50.080.010.071472.31.90.110.030.081482.81.60.120.020.101482.51.70.110.020.091482.91.40.080.010.071472.91.50.120.020.101483.01.50.150.020.131472.61.60.110.020.09149NSNSNS2.71.60.110.020.091480.230.160.0250.0090.0190.7999999Page 559HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-TerminationAnimalGroupNumberK+Cl-Ca++PHOSGGTBileAcidsmEq/LmEq/Lmg/dLmg/dLU/Lumol/L2M20516.010111.38.5065.62000mg/m(3)20525.810310.56.6012.120534.910310.49.9018.620545.610112.18.2016.320555.110311.98.5017.020566.410010.68.0012.920575.59710.77.5016.820586.59811.28.1022.520595.69910.814.5025.12060NSNSNSNSNSNSMean5.710111.18.9023.0SD0.532.20.612.290.016.50n999999Page 560HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-TerminationGroup3M10000mg/m(3}AnimalNumber3051305230533054305530563057305830593060MeanSDnASTU/L97104851056965897969808414.510ALTALKPU/L U/L498233834511651704197271083682419946100439741937.413.91010LDSDHBUNCREATGLUCKU/LU/Lmg/dLmg/dLmg/dLU/L28119.911.70.413729548210.914.40.413948115623.515.10.717815012320.014.50.5131139919.815.90.312419821115.214.40.310612927822.411.50.51452488712.213.60.4120 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562HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryvalues-TerminationAnimalGroupNumberK+Cl-Ca++PHOSGGTBileAcidsmEq/LmEq/Lmg/dLmg/dLU/Lumol/L3M30516.710411.58.8032.510000mg/m(3)30525.510511.27.2024.130534.910210.810.7073.930546.010211.67.0024.930555.710211.17.6023.730566.210011.27.3017.130575.010110.813.6025.430586.210111.38.0017.930596.410010.68.0012.230605.89911.18.1029.2Mean5.810211.18.6028.1SD0.581.80.322.040.017.16n101010101010Page 563HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-TerminationGroup4M20000mg/m(3)AnimalNumber4061406240634064406540664067406840694070MeanSDnASTALTU/LU/L80417540754010459683364305633693371406232723813.18.41010ALKPU/L1237999907993656779808516.910LDSDHBUNCREATGLUCKU/LU/Lmg/dL mg/dLmg/dLU/L17125.716.20.518518513317.013.10.4119 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565HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-TerminationAnimalGroupNumberK+Cl-Ca++PROSGGTBileAcidsmEq/LmEq/Lmg/dLmg/dLU/Lumol/L4M40616.710011.19.6030.020000mg/m(3)40626.310511.18.6021.940635.810310.910.3014.840645.110711.212.1035.040655.910311.08.0024.640665.010110.58.1025.040675.79810.97.5013.540685.710010.87.2013.940695.69810.77.5224.940706.710010.98.8010.9Mean5.910210.98.8021.5SD0.583.00.211.520.67.94n101010101010Page 566HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-TerminationGroup1F0mg/m(3)AnimalNumber1561156215631564156515661567156815691570MeanSDnASTU/L829010491781347010480809118.610ALTALKPU/LU/L4560382738473248354363422636384248505230424310.79.71010LDSDHBUNCREATGLUCKU/LU/Lmg/dLmg/dL mg/dLU/L24718.515.20.411113234317.312.00.511414443414.616.00.512421729916.914.20.61643109912.814.50.51277122525.017.20.513526128115.7 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568HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-TerminationAnimalGroupNumberK+Cl-Ca++PHOSGGTBileAcidsmEq/LmEq/Lmg/dLmg/dLU/Lumol/L1F15615.210411.16.3031.80mg/m(3)15624.710311.75.8025.815634.910810.97.2034.515644.610410.86.9013.915656.110611.46.2011.815664.610311.29.8062.915674.410011.06.9018.015683.710011.16.1094.515694.410011.68.4063.415705.19712.04.8012.0Mean4.810311.36.8036.9SD0.633.30.391.410.027.86n101010101010Page 569HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-TerminationGroup2F2000mg/m(3)AnimalNumber2551255225532554255525562557255825592560MeanSDnASTALTU/LU/L8933882981478130812772298034108638031783584369.811.11010ALKPU/L414433745053395546474811.210LDSDHBUNCREATGLUCKU/LU/Lmg/dLmg/dL mg/dLU/L66910.815.40.512128037914.615.70.412024618317.717.80.6113 10126314.515.40.5120 15844114.018.70.514020215815.3 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571HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-TerminationAnimalGroupNumberK+Cl-Ca++PHOSGGTBileAcidsmEq/LmEq/Lmg/dLmg/dLU/Lumol/L2F25515.710611.76.6042.22000mg/m(3)25524.910611.56.5013.225535.910412.36.0018.225545.710611.46.3016.825555.210511.58.2036.125566.29910.66.1015.725575.19811.55.9081.525586.310011.06.409.325596.210611.88.4017.725605.79912.27.3078.6Mean5.710311.66.8032.9SD0.493.40.510.900.026.86n101010101010Page 572HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-TerminationGroup3F10000mg/m(3)AnimalNumber3551355235533554355535563557355835593560MeanSDnASTU/L83636761787464765885719.610ALTALKPU/LU/L365731293331303437393440353937382552356733433.712.21010LDSDHBUNCREATGLUCKU/LU/Lmg/dLmg/dLmg/dLU/L22521.916.10.51221988411.413.70.4130689812.4 15.40.51137411512.2 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574HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-TerminationAnimalGroupNumberK+Cl-Ca++PHOSGGTBileAcidsmEq/LmEq/Lmg/dLmg/dLU/Lumol/L3F35515.310711.78.8046.110000mg/m(3)35525.510311.46.6013.635536.310511.66.3014.835544.89912.26.4053.635555.810511.36.3022.435565.910111.66.5013.435575.110111.45.8012.135586.010111.46.7021.135595.210411.69.6030.835605.310311.48.6019.3Mean5.510311.67.2024.7SD0.472.40.261.320.014.47n101010101010Page 575HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-TerminationGroup4F20000mg/m(3)AnimalNumber4561456245634564456545664567456845694570MeanSDnASTU/L886996746587697058707511.910ALTALKPU/LU/L413429so72453943294936373144313825463152364413.45.91010LDU/L153831061206674666781488631.310SDHBUNCREATGLUCKU/Lmg/dLmg/dLmg/dLU/L16.713.50.511310011.416.60.51068421.915.30.51677112.814.10.514512411.115.70.51196913.216.50.41357311.119.60.51285510.715.50.51287610.812.70.51291087.016.70.512110812.715.60.5129874.051.950.0317.322.01010101010Page 576HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryvalues-TerminationGroup4F20000mg/m(3)AnimalNumber4561456245634564456545664567456845694570MeanSDnCHOLTPmg/dLg/dL677.5937.7758.4858.0977.6948.31018.4987.41128.5867.4917.913.00.451010ALBg/dL4.95.05.35.04.95.55.74.55.54.75.10.3910GlobA/GTBILIDBILIIBILINa+g/dLmg/dL mg/dLmg/dLmEq/L2.61.90.130.020.111492.71.90.120.040.081503.11.70.140.040.101533.01.70.090.030.061532.71.80.150.040.111542.82.00.130.030.101492.7 2.10.130.030.101482.91.60.120.020.101503.01.80.170.030.141492.71.70.150.040.111492.81.80.130.030.101500.170.150.0220.0080.0212.1101010101010Page 577HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-TerminationAnimalGroupNumberK+Cl-Ca++PROSGGTBileAcidsmEq/LmEq/Lmg/dLmg/dLU/Lumol/L4F45616.810411.57.2113.920000mg/m(3)45625.810311.86.5017.945635.510613.08.30127.645646.710912.27.6018.545655.610711.96.4016.445665.910011.98.0080.345675.59711.97.50165.645686.110311.57.6053.745696.010011.75.2114.445706.510111.46.8032.1Mean6.010311.97.1054.0SD0.483.70.460.910.453.95n101010101010Page 578HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-RecoveryAnimalGroupNumberASTALTALKPLDSDHBUNCREATGLUCKU/LU/LU/LU/LU/Lmg/dL mg/dLmg/dLU/L1M105186449611418.711.40.3107940mg/m(3)10523444826869.813.00.412136510536848899217.011.80.39891105493447927019.913.70.4991751055734210015316.811.30.399109105692426212619.313.60.4104941057193 1157919329.913.60.41482191058704010211218.111.90.3113101105992417319025.913.30.4193161106088516715820.313.00.498127Mean89518320919.612.70.4118 154SD40.722.713.7175.35.380.960.0530.585.6n101010101010101010Page 579HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-RecoveryAnimalGroupNumberCHOLTPALBGlobA/GTBILIDBILIIBILINa+mg/dLg/dL g/dL g/dLmg/dLmg/dLmg/dLmEq/L1M1051917.04.03.01.30.130.030.10145omg/m{3)1052627.04.22.81.50.130.030.101421053756.84.02.81.40.130.040.091481054787.44.62.81.60.170.060.111451055616.84.22.61.60.110.040.071461056817.34.23.11.40.120.020.101461057566.74.12.61.60.110.030.081471058587.24.13.11.30.110.040.071471059576.94.32.61.70.150.030.121471060516.94.22.71.60.120.040.08148Mean677.04.22.81.50.130.040.09146SD13.20.230.170.200.140.0190.0110.0171.8n101010101010101010Page 580HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-RecoveryAnimalGroupNumberK+Cl-Ca++PROSGGTBileAcidsmEq/L mEq/Lmg/dLmg/dLU/Lumol/L1M10516.910311.48.8033.5omg/m{3)10526.610111.07.7016.610536.110211.67.3066.510545.710311.57.3129.510555.810311.07.3012.910566.610411.28.1034.510575.210510.87.9174.010586.410311.06.7013.710595.910611.68.6075.610606.210511.27.9036.3Mean6.110411.27.8039.3SD0.511.50.280.640.424.25n101010101010Page 581HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-RecoveryGroup4M20000mg/m(3)AnimalNumber4051405240534054405540564057405840594060MeanSDnASTALTU/LU/L865668387039684362296739814465608647633872439.2 9.11010ALKPU/L90918882848377809198866.310LDSDHBUNCREATGLUCKU/LU/Lmg/dLmg/dL mg/dLU/L16918.5 13.10.41852289214.19.80.3101817314.012.90.3102829715.612.90.410510013314.313.80.485 897310.314.40.394905912.1 12.50.4118736918.914.00.3116736815.810.60.31027310913.512.00.3119 1119414.712.60.311310034.62.641.460.0527.646.7101010101010Page 582HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-RecoveryAnimalGroupNumberCHOLTPALBGlobA/GTBILIDBILIIBILlNa+mg/dLg/dLg/dLg/dLmg/dL mg/dLmg/dLmEq/L4M4051846.94.12.81.50.130.010.1214720000mg/m(3)4052726.94.32.61.70.150.040.111494053627.04.22.81.50.13 0.03 0.101494054806.84.02.81.40.120.020.101484055586.84.22.61.60.130.050.081494056537.14.42.71.60.130.040.091484057386.84.42.41.80.110.040.071504058967.04.22.81.50.140.040.101484059436.54.22.31.80.140.030.111474060816.84.02.81.40.11 0.02 0.09147Mean676.94.22.71.60.13 0.03 0.10148SD19.00.160.140.180.150.0130.0120.0151.0n1010 10101010 10 10 10Page 583HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-RecoveryAnimalGroupNumberK+Cl-Ca++PHOSGGTBileAcidsmEq/LmEq/Lmg/dLmg/dLU/Lumol/L4M40515.610511.88.1031.120000mg/m(3}40526.310411.07.2143.340536.010511.47.4025.340546.410610.46.6042.940556.210610.87.6037.340566.110411.37.2040.140576.210710.77.3011.840586.410311.57.5022.140596.310610.97.7038.240606.010711.47.6017.3Mean6.210511.17.4030.9SD0.241.30.430.390.311.23n101010101010Page 584HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-RecoveryAnimalGroupNumberASTALTALKPLDSDHBUNCREATGLUCKU/LU/LU/LU/LU/Lmg/dL mg/dLmg/dLU/L1F155111176209523.914.10.598660mg/m(3)155297636614019.115.50.410796155397573624620.115.60.595127155472364822916.114.80.4113131155570494413618.010.80.410294155686404612016.611.40.59512715577731 368511.916.20.4889615583211754419240.615.10.410591155977433411514.1 13.50.497105156080464013613.8 13.80.49492Mean109624114919.4 14.10.499103SD75.742.011.854.98.211.790.057.420.4n101010101010101010Page 585HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-RecoveryAnimalGroupNumberCHOLTPALBGlobA/GTBILIDBILIIBILINa+mg/dLg/dLg/dL g/dLmg/dLmg/dLmg/dLmEq/L1F15511459.06.03.02.00.200.050.151450mg/m(3)1552947.24.92.32.10.180.050.131491553757.64.82.81.70.160.030.131471554897.44.72.71.70.190.040.151461555937.65.22.42.20.180.030.151491556787.44.92.52.00.170.040.1314915571048.35.52.82.00.180.070.111461558817.34.82.51.90.220.060.161491559947.04.82.22.20.140.030.1114915601077.04.32.71.60.140.030.11148Mean967.65.02.61.90.180.040.13148SD20.10.620.470.250.210.0250.0140.0191.6n101010101010101010Page 586HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-RecoveryAnimalGroupNumberK+Cl-Ca++PHOSGGTBileAcidsmEq/LmEq/Lmg/dL mg/dLU/Lumol/L1F15515.310112.55.6063.70mg/m{3)15525.710811.47.1019.615536.110511.56.1014.515545.410311.37.0061.415555.610711.66.1018.015565.910911.46.6013.415575.110312.05.5052.415586.010811.56.9257.615595.910911.36.9116.615606.010811.06.9024.4Mean5.710611.66.5034.2SD0.342.90.420.600.721.58n101010101010Page 587HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-RecoveryAnimalGroupNumberASTALTALKPLDSDHBUNCREATGLUCKU/LU/LU/LU/LU/Lmg/dLmg/dLmg/dLU/L4F4551512928508.012.70.41075520000mg/m(3)45526038268710.613.20.495664553704033529.715.40.5103624554543351549.214.10.51095745558870327820.017.40.41006845569950509715.417.80.58512245579769395813.615.50.592794558603540549.914.00.5105644559773357578.619.10.410710745601491134310123.412.30.512587Mean8151406912.815.20.510377SD29.626.310.419.95.252.310.0510.922.4n10 1010 10 1010101010Page 588HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-RecoveryGroup4F20000mg/m(3)AnimalNumber4551455245534554455545564557455845594560MeanSDnCHOLTPmg/dLg/dL747.8958.0967.7948.1838.51057.1798.1707.6537.51158.5867.918.20.441010ALBg/dL5.45.55.05.55.74.64.54.84.85.65.10.4510GlobA/GTBILIDBILIIBILINa+g/dLmg/dLmg/dLmg/dLmEq/L2.4 2.30.180.050.131482.52.20.190.050.141492.71.90.160.030.131482.62.10.180.050.131482.82.00.140.020.121472.51.80.160.030.131503.61.30.160.030.131522.81.70.080.020.061502.71.80.240.080.161502.91.90.170.040.131492.81.90.170.040.131490.340.280.0400.0180.0251.4101010101010Page 589HuntingdonLifeSciencesStudyNo.:00-6130SponsorStudyNo.:211-DIPE-SAppendixKIndividualClinicalChemistryValues-RecoveryAnimalGroupNumberK+Cl-Ca++PHOSGGTBileAcidsmEq/LmEq/Lmg/dLmg/dLU/Lumol/L4F45516.110612.36.5030.620000mg/m(3)45525.710511.66.4067.745535.610611.44.9031.045545.510611.65.6060.545555.210511.75.4043.145565.911011.26.8219.545575.010812.06.6017.745585.310512.06.1127.745595.510811.77.30210.845605.010612.76.4031.7Mean5.510711.86.2054.0SD0.361.60.440.710.757.45n101010101010Page 590

# Huntingdon Life Sciences 00

- 6130 Page 591

211

- DIPE

- S Final Report

Individual Organ Weights and

Brain Measurements

Preface Appendix L

Absolute Organ Weights (Terminal Sacrifice)

................................................... 592

% Organ to Body Weight (Terminal Sacrifice)

.................................................. 600

% Organ to Brain Weight (Terminal Sacrifice)

.................................................. 608

Brain Measurements (Terminal Sacrifice)

....................................................... 616

Absolute Organ Weights (Recovery Sacrifice)

.................................................. 618

% Organ to Body Weight (Recovery Sacrifice)

................................................. 622

% Organ to Brain Weight (Recovery Sacrifice)

................................................. 626

Note: Brain length and width were measured in centimeters.

Corresponding exposure levels for each group were as follows:

Group I

- 0 mg/m 3



# Group II

- 2000 mg/m 3



# Group III

- 10000 mg/m 3



# Group I V - 20000 mg/m 3



# Key to Abbreviations

g = Grams

wt. = Weight

observ. = ObservationsHuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalSummarystatisticsforabsoluteorganweights(g)Studynumber:006130BrainHeartNo/sexSubgroupBodywt.(g)AdrenalGlandsEpididymis1061/M1/11062/M1/11063/M1/11064/M1/11065/M1/11066/M1/11067/M1/11068/M1/11069/M1/11070/M1/1Mean:Standarddeviation:Numberofobserv.2051/M2052/M2053/M2054/M2055/M2056/M2057/M2058/M2059/M2/12/12/12/12/12/12/12/12/1Me an:Standarddeviation:Numberofobserv.3051/M3052/M3053/M3054/M3055/M3056/M3057/M3058/M3059/M3060/M3/13/13/13/13/13/13/13/13/13/1Me an:Standarddeviation:Numberofobserv.4061/M4062/M4063/M4064/M4/14/14/14/1564.5523.3540.7541.2523.2549.9509.9505.6493.1537.6528.921.9(10)441.7485.6507.2487.5522.2509.9494.6557.9549.5506.235.2(9)501.2530.0497.1530.2517.9456.3628.4501.8505.6475.9514.446.0(10)505.3426.4542.9504.20.06760.06130.07550.08380.07390.07770.07020.07570.06470.07450.07250.0066(10)0.07860.08620.07130.07510.08340.07740.05790.06720.05630.07260.0105(9)0.05630.06460.05830.07380.07310.07150.09630.07120.05410.06100.06800.0123(10)0.06780.06970.07260.0832Male2.40632.12252.25402.15702.25162.28922.25002.18902.04852.18702.21550.0985(10)2.04322.16772.32362.09072.10002.06102.20392.05412.28522.14770.1038(9)2.08712.22852.22772.16002.19831.98842.22982.16212.21042.18152.16740.0766(10)2.24712.06652.23832.2660Anim als1.85981.65931.42141.68131.36631.49921.56571.67631.37461.66101.57650.1600(10)1.38041.61011.77781.36832.07331.66611.54751.45961.82911.63470.2308(9)1.51311.53471.72081.54311.60421.58801.46221.64001.48110.29141.43790.4102(10)1.30421.51351.60821.53391.76941.84431.67381.97931.73491.60461.73201.55071.60531.92781.74220.1418(10)1.54081.67491.54481.60061.72231.85621.42871.72971.81141.65660.1391(9)1.81681.88961.51611.67161.53331.57941.99411.61231.52731.47321.66140.1786(10)1.57511.36851.70951.5118Printed:25-Mar-04Page:1Kidneys4.79824.92064.41695.08204.54093.83023.89603.92803.67314.37804.34640.4963(10)3.58984.10164.27744.32194.39354.46373.94834.27614.96044.25920.3751(9)4.72374.74453.91524.14135.14894.12525.05374.63034.21964.03594.47380.4413(10)4.67223.81304.51425.0286Version4.2.2Liver17.062116.341015.640316.395317.166416.967317.724512.905113.065915.077315.83451.6854(10)13.034413.862113.740113.573915.609814.212912.742317.796617.808914.70901.9306(9)14.515814.490813.299016.266415.213713.179217.824816.856115.332813.518215.04971.5655(10)15.321011.449715.983713.5426Page 592HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalSummarystatisticsforabsoluteorganweights(g)Studynumber:006130BrainHeartNo/sexSubgroupBodywt.(g)AdrenalGlandsEpididymisKidneysPrinted:25-Mar-04Page:2Version4.2.2Liver-----------------------------------------------------------------------------------------------------------------------------------4065/M4/1497.70.06152.23381.52651.47634.320814.55134066/M4/1526.60.06402.09451.69991.84484.711117.57594067/M4/1513.30.07122.22131.85511.63374.566616.98184068/M4/1648.20.08262.11761.84952.19545.233520.40464069/M4/1458.30.05871.99401.81521.39463.938213.07984070/M4/1543.20.07012.15951.55321.72704.885816.5023M e an:516.60.07012.16391.62591.64374.568415.5393Standarddeviation:58.70.00800.09210.17760.24590.44952.5562Numberofobserv.:(10)(10)(10)(10)(10)(10)(10)Page 593HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalSummarystatisticsforabsoluteorganweights(g)Studynumber:006130ProstateSpleenNo/sexSubgroupBodywt.(g)LungsSeminalvesicles1061/M1/11062/M1/11063/M1/11064/M1/11065/M1/11066/M1/11067/M1/11068/M1/11069/M1/11070/M1/1Me an:Standarddeviation:Numberofobserv.2051/M2052/M2053/M2054/M2055/M2056/M2057/M2058/M2059/M2/12/12/12/12/12/12/12/12/1Me an:Standarddeviation:Numberofobserv.3051/M3052/M3053/M3054/M3055/M3056/M3057/M3058/M3059/M3060/M3/13/13/13/13/13/13/13/13/13/1M e an:Standarddeviation:Numberofobserv.4061/M4062/M4063/M4064/M4/14/14/14/1564.5523.3540.7541.2523.2549.9509.9505.6493.1537.6528.921.9(10)441.7485.6507.2487.5522.2509.9494.6557.9549.5506.235.2(9)501.2530.0497.1530.2517.9456.3628.4501.8505.6475.9514.446.0(10)505.3426.4542.9504.22.87492.45192.17972.79402.37082.03462.08372.06132.11872.03812.30080.3150(10)2.04421.95182.32822.26571.90072.23641.92911.94422.24582.09400.1722(9)1.83892.42632.25762.13912.26862.04842.62911.95591.95801.97802.15000.2456(10)2.06761.64832.04002.0895Ma1e1.03870.96581.35731.28731.75540.79821.04361.22061.06310.80061.13310.2870(10)1.02241.21701.09371.37401.16860.86100.79790.93121.22331.07660.1893(9)0.98671.46371.59450.86031.21221.02451.13111.29891.59161.32871.24920.2533(10)0.98681.02171.27510.9505A nim a 1 s1.70281.87011.24401.52481.94992.22421.57421.90771.41491.86611.72790.2915(10)1.11521.51541.50521.27331.59661.17801.48521.30451.81991.42150.2231(9)2.21611.19971.26462.13422.50351.80201.49892.25411.79690.83601.75060.5404(10)1.49001.35502.02652.21620.76520.66410.63450.83190.85420.70460.71950.73590.77581.05860.77440.1209(10)0.73290.93850.96930.91290.64631.02850.80440.80950.73480.84190.1272(9)0.81951.02800.61200.97950.67730.69640.76220.76520.74470.86630.79510.1315(10)0.57250.56670.89700.6757Printed:25-Mar-04Page:3Testes3.88053.55173.29664.46502.60433.69833.35093.39713.29094.55803.60930.5810(10)3.17593.37433.83693.05523.57683.60013.86253.28603.08923.42850.3058(9)3.24024.20693.29383.72033.88553.74383.54224.18033.10681.26963.41890.8429(10)3.58934.06993.75413.5838Version4.2.2Thymus0.52740.42410.34000.33470.27460.43900.34630.27220.27430.46400.36970.0893(10)0.24330.31130.44140.41580.45780.40270.39860.48090.36010.39020.0750(9)0.40700.34410.30340.43140.33140.41430.43720.35350.25970.28110.35630.0638(10)0.37110.23210.44340.2529Page 594HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalSummarystatisticsforabsoluteorganweights(g)Studynumber:006130ProstateSpleenNo/sexSubgroupBodywt.(g)LungsSeminalvesicles4065/M4/1497.71.74490.78341.82610.94354066/M4/1526.62.06521.55701.38580.84434067/M4/1513.32.10941.27871.16470.74634068/M4/1648.22.62911.45481.81860.91934069/M4/1458.31.67571.12681.86780.63864070/M4/1543.21.98541.08192.26290.9476M e an:516.62.00551.15171.74140.7752Standarddeviation:58.70.28280.23880.37700.1536Numberofobserv.:(10)(10)(10)(10)(10)Printed:25-Mar-04Page:4Version4.2.2ThymusTestes3.58310.32413.85500.39603.58650.32164.08540.40083.96850.30343.29300.30043.73690.33460.25580.0674(10)(10)Page 595HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalSummarystatisticsforabsoluteorganweights(g)Studynumber:006130BrainKidneysNo/sexSubgroupBodywt.(g)AdrenalGlandsHeart1561/F1/11562/F1/11563/F1/11564/F1/11565/F1/11566/F1/11567/F1/11568/F1/11569/F1/11570/F1/1Me an:Standarddeviation:Numberofobserv.2551/F2552/F2553/F2554/F2555/F2556/F2557/F2558/F2559/F2560/F2/12/12/12/12/12/12/12/12/12/1Me an:Standarddeviation:Numberofobserv.3551/F3/13552/F3/13553/F3/13554/F3/13555/F3/13556/F3/13557/F3/13558/F3/13559/F3/13560/F3/1Me 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596HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalSummarystatisticsforabsoluteorganweights(g)Studynumber:006130BrainKidneysNo/sexSubgroupBodywt.(g)AdrenalGlandsHeart4564/F4/1318.20.07802.10921.12282.91074565/F4/1247.00.05491.95490.87091.87954566/F4/1285.90.06252.12970.98042.45474567/F4/1324.70.08462.13511.11432.58234568/F4/1280.10.07912.09660.84792.40354569/F4/1299.50.04962.19191.08882.52914570/F4/1283.50.06232.01191.06052.0879M e an:284.60.06962.07941.00792.4240Standarddeviation:28.30.01150.06920.09430.2768Numberofobserv.:(10)(10)(10)(10)(10)Printed:30-Mar-04Page:2Version4.2.2LungsLiver9.59621.79157.39661.21007.91311.444411.36651.56988.56741.69099.05781.61878.17691.46528.82501.60561.09680.2027(10)(10)Page 597HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalSummarystatisticsforabsoluteorganweights(g)Studynumber:006130SpleenUterusNo/sexSubgroupBodywt.(g)OvariesThymusF e m a 1 e A n i m a 1 s1561/F1/1290.50.11750.61160.43400.76371562/F1/1291.00.10750.53110.33761.25601563/F1/1292.30.09820.58950.29590.81801564/F1/1285.10.08260.52240.25621.14731565/F1/1282.90.09880.58050.29480.91211566/F1/1267.50.09870.55880.29220.73641567/F1/1297.30.09500.63760.31050.93741568/F1/1359.80.08580.54810.31240.88511569/F1/1282.60.08060.68370.35410.66951570/F1/1291.20.06390.50430.30190.8581M e an:294.00.09290.57680.31900.8984Standarddeviation:24.50.01520.05570.04830.1815Numberofobserv.(10)(10)(10)(10)(10)2551/F2/1294.50.11590.62050.36030.70802552/F2/1273.10.08300.51840.29510.65362553/F2/1281.80.08770.48490.31750.61312554/F2/1287.00.11330.58250.34330.77492555/F2/1287.50.09240.56440.40260.65642556/F2/1301.10.08280.50580.32150.72902557/F2/1338.70.06960.62340.26410.73782558/F2/1249.00.09470.49540.26420.57612559/F2/1262.00.08630.39640.41460.69152560/F2/1310.20.09520.71020.35380.7191M e an:288.50.09210.55020.33370.6860Standarddeviation:25.20.01400.08890.05170.0609Numberofobserv.:(10)(10)(10)(10)(10)3551/F3/1323.00.10610.66530.34570.67843552/F3/1306.20.11660.59660.38320.61393553/F3/1265.70.09420.52390.22950.83593554/F3/1358.10.06520.71740.33760.85133555/F3/1288.40.09550.68910.33760.83703556/F3/1324.40.09280.57480.47361.57853557/F3/1267.10.06920.43060.31190.85973558/F3/1289.60.08300.50670.31321.78853559/F3/1352.20.10070.59140.33200.86503560/F3/1316.30.09130.58550.32811.1408M e an:309.10.09150.58810.33921.0049Standarddeviation:32.00.01570.08740.06120.3861Numberofobserv.:(10)(10)(10)(10)(10)4561/F4/1302.80.09890.72580.35190.60594562/F4/1238.90.10620.49730.39570.87834563/F4/1265.10.08870.59800.26580.6781Printed:30-Mar-04Page:3Version4.2.2Page 598HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalSummarystatisticsforabsoluteorganweights(g)Studynumber:006130SpleenUterusNo/sexSubgroupBodywt.(g)OvariesThymus4564/F4/1318.20.11010.57140.29400.58144565/F4/1247.00.06780.54180.14210.77184566/F4/1285.90.08590.47130.28040.65854567/F4/1324.70.07900.59850.35600.90374568/F4/1280.10.07710.65470.32700.87584569/F4/1299.50.09200.51990.28210.60794570/F4/1283.50.08420.59200.29551.4417M e an:284.60.08900.57710.29910.8003Standarddeviation:28.30.01320.07550.06880.2566Numberofobserv.:(10)(10)(10)(10)(10)Printed:30-Mar-04Page:4Version4.2.2Page 599HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalSummaryStatisticsfor%OrgantoBodyWeightStudynumber:006130BrainHeartNo/sexSubgroupBodywt.(g)AdrenalGlandsEpididymis1061/M1/11062/M1/11063/M1/11064/M1/11065/M1/11066/M1/11067/M1/11068/M1/11069/M1/11070/M1/1Me an:Standarddeviation:Numberofobserv.2051/M2052/M2053/M2054/M2055/M2056/M2057/M2058/M2059/M2/12/12/12/12/12/12/12/12/1Me an:Standarddeviation:Numberofobserv.3051/M3052/M3053/M3054/M3055/M3056/M3057/M3058/M3059/M3060/M3/13/13/13/13/13/13/13/13/13/1Me 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600HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalSummaryStatisticsfor%OrgantoBodyWeightStudynumber:006130BrainHeartNo/sexSubgroupBodywt.(g)AdrenalGlandsEpididymis4065/M4/1497.70.01240.44880.30670.29664066/M4/1526.60.01220.39770.32280.35034067/M4/1513.30.01390.43270.36140.31834068/M4/1648.20.01270.32670.28530.33874069/M4/1458.30.01280.43510.39610.30434070/M4/1543.20.01290.39760.28590.3179M e an:516.60.01360.42300.31720.3174Standarddeviation:58.70.00150.04290.04190.0167Numberofobserv.:(10)(10)(10)(10)(10)Printed:25-Mar-04Page:2Version4.2.2LiverKidneys0.86822.92370.89463.33760.88973.30840.80743.14790.85932.85400.89943.03800.88662.99570.05210.2264(10)(10)Page 601HuntingdonLifeSciencesSummaryStatisticsfor%OrgantoBodyWeightPrincetonResearchCenterStudynumber:006130EastMillstone,NewJerseyAppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalProstateSpleenNo/sexSubgroupBodywt.(g)LungsSeminalvesicles1061/M1062/M1063/M1064/M1065/M1066/M1067/M1068/M1069/M1070/M1/11/11/11/11/11/11/11/11/11/1Me an:Standarddeviation:Numberofobserv.2051/M2052/M2053/M2054/M2055/M2056/M2057/M2058/M2059/M2/12/12/12/12/12/12/12/12/1Me an:Standarddeviation:Numberofobserv.3051/M3052/M3053/M3054/M3055/M3056/M3057/M3058/M3059/M3060/M3/13/13/13/13/13/13/13/13/13/1Me 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602HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalAnimalGroup/TerminalNo/sexSubgroupBodywt.(g)4065/M4/1497.74066/M4/1526.64067/M4/1513.34068/M4/1648.24069/M4/1458.34070/M4/1543.2M e an:516.6Standarddeviation:58.7Numberofobserv.:(10)SacrificeSummaryStatisticsfor%OrgantoBodyWeightStudynumber:006130ProstateSpleenLungsSeminalvesicles0.35060.15740.36690.18960.39220.29570.26320.16030.41090.24910.22690.14540.40560.22440.28060.14180.36560.24590.40750.13930.36550.19920.41660.17440.38760.22300.33870.14960.02250.03910.07220.0226(10)(10)(10)(10)Printed:25-Mar-04Page:4Version4.2.2ThymusTestes0.71990.06510.73210.07520.69870.06270.63030.06180.86590.06620.60620.05530.73200.06460.10400.0100(10)(10)Page 603HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalSummaryStatisticsfor%OrgantoBodyWeightStudynumber:006130BrainKidneysNo/sexSubgroupBodywt.(g)AdrenalGlandsHeart1561/F1562/F1563/F1564/F1565/F1566/F1567/F1568/F1569/F1570/F1/11/11/11/11/11/11/11/11/11/1Me an:Standarddeviation:Numberofobserv.2551/F2552/F2553/F2554/F2555/F2556/F2557/F2558/F2559/F2560/F2/12/12/12/12/12/12/12/12/12/1Me an:Standarddeviation:Numberofobserv.3551/F3/13552/F3/13553/F3/13554/F3/13555/F3/13556/F3/13557/F3/13558/F3/13559/F3/13560/F3/1Me 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604HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalAnimalGroup/TerminalNo/sexSubgroupBodywt.(g)4564/F4/1318.24565/F4/1247.04566/F4/1285.94567/F4/1324.74568/F4/1280.14569/F4/1299.54570/F4/1283.5M e an:284.6Standarddeviation:28.3Numberofobserv.:(10)SacrificeSummaryStatisticsfor%OrgantoBodyWeightStudynumber:006130BrainKidneysAdrenalGlandsHeart0.02450.66290.35290.91470.02220.79150.35260.76090.02190.74490.34290.85860.02610.65760.34320.79530.02820.74850.30270.85810.01660.73190.36350.84440.02200.70970.37410.73650.02460.73630.35540.85420.00440.06680.02830.0851(10)(10)(10)(10)Printed:25-Mar-04Page:2version4.2.2LungsLiver3.01580.56302.99460.48992.76780.50523.50060.48353.05870.60373.02430.54052.88430.51683.10460.56940.25850.0996(10)(10)Page 605HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalAnimalGroup/TerminalNo/sexSubgroupBodywt.(g)1561/F1/1290.51562/F1/1291.01563/F1/1292.31564/F1/1285.11565/F1/1282.91566/F1/1267.51567/F1/1297.31568/F1/1359.81569/F1/1282.61570/F1/1291.2M e an:294.0Standarddeviation:24.5Numberofobserv.:(10)2551/F2/1294.52552/F2/1273.12553/F2/1281.82554/F2/1287.02555/F2/1287.52556/F2/1301.12557/F2/1338.72558/F2/1249.02559/F2/1262.02560/F2/1310.2M e an:288.5Standarddeviation:25.2Numberofobserv.:(10)3551/F3/1323.03552/F3/1306.23553/F3/1265.73554/F3/1358.13555/F3/1288.43556/F3/1324.43557/F3/1267.13558/F3/1289.63559/F3/1352.23560/F3/1316.3M e an:309.1Standarddeviation:32.0Numberofobserv.:(10)4561/F4/1302.84562/F4/1238.94563/F4/1265.1SacrificeSummaryStatisticsfor%OrgantoBodyWeightStudynumber:006130SpleenUterusOvariesThymusF e m a l eA n i m a l 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606HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalSummaryStatisticsfor%OrgantoBodyWeightStudynumber:006130SpleenUterusNo/sexSubgroupBodywt.(g)OvariesThymus4564/F4/1318.20.03460.17960.09240.18274565/F4/1247.00.02740.21940.05750.31254566/F4/1285.90.03000.16480.09810.23034567/F4/1324.70.02430.18430.10960.27834568/F4/1280.10.02750.23370.11670.31274569/F4/1299.50.03070.17360.09420.20304570/F4/1283.50.02970.20880.10420.5085M e an:284.60.03150.20380.10550.2852Standarddeviation:28.30.00550.02650.02700.0981Numberofobserv.:(10)(10)(10)(10)(10)Printed:25-Mar-04Page:4Version4.2.2Page 607HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalSummaryStatisticsfor%OrgantoBrainWeightStudynumber:006130BrainHeartNo/sexSubgroupBodywt.(g)AdrenalGlandsEpididymis1061/M1062/M1063/M1064/M1065/M1066/M1067/M1068/M1069/M1070/M1/11/11/11/11/11/11/11/11/11/1Me an:Standarddeviation:Numberofobserv.2051/M2052/M2053/M2054/M2055/M2056/M2057/M2058/M2059/M2/12/12/12/12/12/12/12/12/1Me an:Standarddeviation:Numberofobserv.3051/M3/13052/M3/13053/M3/13054/M3/13055/M3/13056/M3/13057/M3/13058/M3/13059/M3/13060/M3/1M e an:Standarddeviation:Numberofobserv.4061/M4062/M4063/M4064/M4/14/14/14/1564.5523.3540.7541.2523.2549.9509.9505.6493.1537.6528.921.9(10)441.7485.6507.2487.5522.2509.9494.6557.9549.5506.235.2(9)501.2530.0497.1530.2517.9456.3628.4501.8505.6475.9514.446.0(10)505.3426.4542.9504.22.80932.88813.34963.88503.28213.39423.12003.45823.15843.40653.27510.3068(10)3.84693.97663.06853.59213.97143.75552.62723.27152.46373.39700.5725(9)2.69752.89882.61703.41673.32533.59594.31883.29312.44752.79623.14070.5634(10)3.01723.37293.24353.6717Ma1e100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.00000.0000(10)100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.00000.0000(9)100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.00000.0000(10)100.0000100.0000100.0000100.0000Anim 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608HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalSummaryStatisticsfor%OrgantoBrainWeightStudynumber:006130BrainHeartNo/sexSubgroupBodywt.(g)AdrenalGlandsEpididymis4065/M4/1497.72.7532100.000068.3365 66.08924066/M4/1526.63.0556100.0000'81.160288.07834067/M4/1513.33.2053100.000083.514273.54704068/M4/1648.23.9006100.000087.3394103.67404069/M4/1458.32.9438100.000091.033169.93984070/M4/1543.23.2461100.000071.924179.9722M e an:516.63.2410100.000075.412876.0710Standarddeviation:58.70.34160.000010.143511.9499Numberofobserv.:(10)(10)(10)(10)(10)Printed:25-Mar-04Page:2Version4.2.2LiverKidneys193.4283651.4147224.9272839.1454205.5823764.4984247.1430963.5720197.5025655.9579226.2468764.1724211.0862718.637918.7674120.2962(10)(10)Page 609HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseySummaryStatisticsfor%OrgantoBrainWeightStudynumber:006130AppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalNo/sexSubgroupBodywt.(g)Lungs1061/M1/11062/M1/11063/M1/11064/M1/11065/M1/11066/M1/11067/M1/11068/M1/11069/M1/11070/M1/1M e an:Standarddeviation:Numberofobserv.2051/M2/12052/M2/12053/M2/12054/M2/12055/M2/12056/M2/12057/M2/12058/M2/12059/M2/1M e an:Standarddeviation:Numberofobserv.3051/M3/13052/M3/13053/M3/13054/M3/13055/M3/13056/M3/13057/M3/13058/M3/13059/M3/13060/M3/1Me 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610HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalAnimalGroup/TerminalNo/sexSubgroupBodywt.(g)4065/M4/1497.74066/M4/1526.64067/M4/1513.34068/M4/1648.24069/M4/1458.34070/M4/1543.2M e an:516.6Standarddeviation:58.7Numberofobserv.:(10)SummaryStatisticsfor%OrgantoBrainWeightStudynumber:006130SacrificeProstateSpleenLungsSeminalvesicles78.113535.070381.748642.237498.601174.337666.163840.310394.962457.565452.433333.5974124.154768.700485.880243.412484.037156.509593.671032.026191.938050.0996104.788243.880592.693353.455280.490235.825912.859311.9996 17.03476.9513(10)(10)(10)(10)Printed:25-Mar-04Page:4Version4.2.2ThymusTestes160.403814.5089184.053518.9067161.459514.4780192.926018.9271199.022115.2156152.489013.9106173.290715.466317.96863.0664(10)(10)Page 611HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalAnimalGroup/TerminalNo/sexSubgroupBodywt.(g)1561/F1/11562/F1/11563/F1/11564/F1/11565/F1/11566/F1/11567/F1/11568/F1/11569/F1/11570/F1/1Me an:Standarddeviation:Numberofobserv.2551/F2552/F2553/F2554/F2555/F2556/F2557/F2558/F2559/F2560/F2/12/12/12/12/12/12/12/12/12/1M e an:Standarddeviation:Numberofobserv.3551/F3/13552/F3/13553/F3/13554/F3/13555/F3/13556/F3/13557/F3/13558/F3/13559/F3/13560/F3/1Me an:Standarddeviation:Numberofobserv.4561/F4562/F4563/F4/14/14/1290.5291.0292.3285.1282.9267.5297.3359.8282.6291.2294.024.5(10)294.5273.1281.8287.0287.5301.1338.7249.0262.0310.2288.525.2(10)323.0306.2265.7358.1288.4324.4267.1289.6352.2316.3309.132.0(10)302.8238.9265.1SacrificeSummaryStatisticsfor%OrgantoBrainWeightStudynumber:006130BrainKidneysAdrenalGlandsHeart2.88654.06173.74282.67453.50533.52953.56532.93443.32033.51153.37320.4254(10)3.96154.70194.08092.67183.48193.71013.57483.66543.29563.96653.71100.5346(10)4.09734.59522.47953.46702.87133.40213.11852.35982.37303.40053.21640.7397(10)3.64753.72793.5484Fe m ale100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.00000.0000(10)100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.00000.0000(10)100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.00000.0000(10)100.0000100.0000100.0000Anim 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612HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalSummaryStatisticsfor%OrgantoBrainWeightStudynumber:006130BrainKidneysNo/sexSubgroupBodywt.(g)AdrenalGlandsHeart4564/F4/1318.23.6981100.000053.2335138.00024565/F4/1247.02.8083100.000044.549696.14304566/F4/1285.92.9347100.000046.0347115.26044567/F4/1324.73.9623100.000052.1896120.94524568/F4/1280.13.7728100.000040.4417114.63804569/F4/1299.52.2629100.000049.6738115.38394570/F4/1283.53.0966100.000052.7114103.7775M e an:284.63.3459100.000048.4561116.3956Standarddeviation:28.30.54340.00004.046111.1258Numberofobserv.:(10)(10)(10)(10)(10)Printed:25-Mar-04Page:2Version4.2.2LungsLiver454.968784.9374378.362161.8958371.559467.8218532.363973.5235408.633180.6496413.239773.8492406.426872.8267423.982877.155546.34709.0447(10)(10)Page 613HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalSummaryStatisticsfor%OrgantoBrainWeightStudynumber:006130SpleenUterusNo/sexSubgroupBodywt.(g)OvariesThymusF e m a 1 e A n i m a 1 s1561/F1/1290.55.981831.135822.094438.87901562/F1/1291.05.655827.942317.761966.08091563/F1/1292.34.823428.955314.534140.17881564/F1/1285.14.002125.311312.413455.58891565/F1/1282.94.891628.740514.595545.15791566/F1/1267.54.726826.761213.993635.26651567/F1/1297.34.671731.354815.269246.09791568/F1/1359.83.958725.288414.413640.83701569/F1/1282.64.104534.816918.032334.09381570/F1/1291.23.125224.663814.765041.9670M e an:294.04.594228.497015.787344.4148Standarddeviation:24.50.84123.22832.77919.7483Numberofobserv.:(10)(10)(10)(10)(10)2551/F2/1294.55.746430.765017.864035.10342552/F2/1273.13.990424.923114.187531.42312553/F2/1281.84.030322.284014.591028.17562554/F2/1287.05.544128.503616.798837.91842555/F2/1287.54.401326.883919.176931.26612556/F2/1301.13.974124.276515.430834.98922557/F2/1338.73.269429.284112.406134.65802558/F2/1249.05.211927.264714.540531.70612559/F2/1262.03.972218.245419.083131.82822560/F2/1310.24.610634.395617.134834.8266M e an:288.54.475126.682616.121333.1895Standarddeviation:25.20.79694.54652.24832.7911Numberofobserv.:(10)(10)(10)(10)(10)3551/F3/1323.04.945731.012016.114331.62263552/F3/1306.25.622328.767118.477329.60123553/F3/1265.74.246723.618210.346237.68373554/F3/1358.13.157134.737616.347141.22123555/F3/1288.44.819134.773217.035942.23653556/F3/1324.44.283826.533721.862272.86623557/F3/1267.13.366620.948715.173941.82443558/F3/1289.63.878523.677614.635583.57483559/F3/1352.24.586726.936915.121839.39883560/F3/1316.34.599529.496216.529057.4710M e an:309.14.350628.050116.164347.7500Standarddeviation:32.00.74084.64312.925017.8805Numberofobserv.:(10)(10)(10)(10)(10)4561/F4/1302.84.888135.872117.392429.94614562/F4/1238.95.101823.890319.009442.19354563/F4/1265.14.305629.027712.902332.9159Printed:25-Mar-04Page:3Version4.2.2Page 614HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:MainStudy-TerminalSacrificeAnimalGroup/TerminalSummaryStatisticsfor%OrgantoBrainWeightStudynumber:006130SpleenUterusNo/sexSubgroupBodywt.(g)OvariesThymus4564/F4/1318.25.220027.090813.938927.56504565/F4/1247.03.468227.71507.268939.48034566/F4/1285.94.033422.129913.166230.91994567/F4/1324.73.700128.031516.673742.32594568/F4/1280.13.677431.226715.596741.77244569/F4/1299.54.197323.719212.870127.73394570/F4/1283.54.185129.424914.687671.6586M e an:284.64.277727.812814.350638.6511Standarddeviation:28.30.61144.02353.232313.0661Numberofobserv.:(10)(10)(10)(10)(10)Printed:25-Mar-04Page:4Version4.2.2Page 615HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:TerminalSacrificeAnimalGroup/TerminalNo/sexSubgroupBodywt.(g)1071/M1/1676.41072/M1/1550.11073/M1/1557.61074/M1/1480.01075/M1/1528.8M e an:558.6Standarddeviation:72.5Numberofobserv.:(5)2061/M2/1506.02062/M2/1573.52063/M2/1605.42064/M2/1472.02065/M2/1649.1M e an:561.2Standarddeviation:72.2Numberofobserv.:(5)3061/M3/1619.53062/M3/1627.33063/M3/1577.13064/M3/1597.33065/M3/1496.6M e an:583.6Standarddeviation:52.4Numberofobserv.:(5)4071/M4/1516.64072/M4/1629.84073/M4/1586.74074/M4/1452.94075/M4/1526.3M e an:542.5Standarddeviation:68.1Numberofobserv.:(5)Summarystatisticsforabsoluteorganweights(g)Studynumber:006130N-NeurotoxBrainMeasurementsLengthweightWidthM a 1 e A n i m a 1 s1.84302.21.51.78902.11.41.65002.11.51.46002.11.51.66702.11.51.68182.11.50.14820.00.0(5)(5)(5)1.79802.11.61.72702.11.51.73402.21.51.66102.21.51.84602.21.51.75322.21.50.07100.10.0(5)(5)(5)1.92902.11.51.77002.21.51.68802.21.51.89502.21.51.66602.11.51.78962.21.50.11890.10.0(5)(5)(5)1.79602.21.51.80302.11.51.83502.11.51.69802.11.51.62402.21.51.75122.11.50.08760.10.0(5)(5)(5)Printed:25-Mar-04Page:1Version4.2.2Page 616HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:TerminalSacrificeAnimalGroup/TerminalNo/sexSubgroupBodywt.(g)1571/F1/1316.61572/F1/1324.21573/F1/1385.81574/F1/1348.91575/F1/1277.1M e an:330.5Standarddeviation:40.3Numberofobserv.:(5)2561/F2/1287.02562/F2/1355.82563/F2/1318.12564/F2/1316.12565/F2/1315.7M e an:318.5Standarddeviation:24.5Numberofobserv.:(5)3561/F3/1316.33562/F3/1259.93563/F3/1269.73564/F3/1344.73565/F3/1388.9M e an:315.9Standarddeviation:53.5Numberofobserv.:(5)4571/F4/1364.34572/F4/1309.94573/F4/1307.84574/F4/1283.54575/F4/1318.2M e an:316.7Standarddeviation:29.6Numberofobserv.:(5)Summarystatisticsforabsoluteorganweights(g)Studynumber:006130N-NeurotoxBrainMeasurementsLengthWeightWidthF e m a 1 eA n i m a 1 s1.64202.11.51.59602.11.5l.65402.11.51.68502.11.51.67002.11.4l.64942.11.50.03400.00.0(5)(5)(5)1.67202.11.51.74902.11.51.67502.11.51.69902.11.5l.70402.11.51.69982.11.50.03090.00.0(5)(5)(5)1.64202.11.4l.91302.11.5l.50102.11.31.69802.11.51.63902.21.51.67862.11.40.14980.00.1(5)(5)(5)1.74102.11.51.71502.21.41.58902.11.41.76202.11.41.72202.11.41.70582.11.40.06780.00.0(5)(5)(5)Printed:25-Mar-04Page:1Version4.2.2Page 617HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:RecoverySacrificeAnimalGroup/TerminalNo/sexSubgroupBodywt.(g)1051/M1052/M1053/M1054/M1055/M1056/M1057/M1058/M1059/M1060/M1/11/11/11/11/11/11/11/11/11/1M e an:Standarddeviation:Numberofobserv.4051/M4/14052/M4/14053/M4/14054/M4/14055/M4/14056/M4/14057/M4/14058/M4/14059/M4/14060/M4/1Me an:Standarddeviation:Numberofobserv.:559.1523.7559.3509.5516.7587.2503.5608.6535.9501.9540.536.8(10)568.5532.4565.0533.3488.0520.2566.7554.5520.7586.9543.629.8(10)Summarystatisticsforabsoluteorganweights(g)Studynumber:006130AdrenalGlands0.06030.07980.05990.07930.05490.06370.08550.07630.05980.06600.06860.0107(10)0.06210.06650.06310.07000.09040.06010.07530.06280.06960.07100.06910.0089(10)BrainMa1e2.21502.11652.29352.05692.23362.30812.33882.31232.36162.17992.24160.1002(10)2.17382.11622.33602.30302.16552.36112.31062.18462.33812.34352.26320.0920(10)EpididymisAnim a1s1.51851.70801.41241.53511.55241.51941.53221.59631.58901.58011.55430.0751(10)1.61301.66751.73391.76211.79101.30801.58821.63001.68561.57811.63570.1361(10)Heart1.98961.54971.56051.53441.55581.85001.64291.81421.90681.58501.69890.1729(10)1.78571.77881.89361.77331.58791.59631.92011.46541.70111.78161.72840.1421(10)Printed:25-Mar-04Page:1Kidneys4.41184.09183.81943.74164.65775.11554.17964.85394.66344.08924.36240.4534(10)4.34193.93864.09194.78594.16994.53425.09544.42585.41515.46454.62630.5443(10)Version4.2.2Liver15.535414.522913.496814.074115.840515.549313.674017.341113.214115.406714.86551.2932(10)16.109713.883215.244815.220815.088614.075616.364616.108117.137818.426415.76601.3737(10)Page 618HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:RecoverySacrificeAnimalGroup/TerminalNo/sexSubgroupBodywt.(g)1051/M1052/M1053/M1054/M1055/M1056/M1057/M1058/M1059/M1060/M1/11/11/11/11/11/11/11/11/11/1Me an:Standarddeviation:Numberofobserv.4051/M4/14052/M4/14053/M4/14054/M4/14055/M4/14056/M4/14057/M4/14058/M4/14059/M4/14060/M4/1M e an:Standarddeviation:Numberofobserv.:559.1523.7559.3509.5516.7587.2503.5608.6535.9501.9540.536.8(10)568.5532.4565.0533.3488.0520.2566.7554.5520.7586.9543.629.8(10)Summarystatisticsforabsoluteorganweights(g)Studynumber:006130ProstateSpleenLungsSeminalvesicles2.20791.86101.97052.58071.85051.92942.15411.80282.12291.75172.02320.2499(10)1.98272.27082.38572.22572.00691.97622.22171.83332.00062.07852.09820.1702(10)Ma1e1.12631.31190.79131.30591.63641.23441.71021.26731.06521.33871.27880.2642(10)1.07031.12221.15761.25161.29770.99841.25370.96211.60241.12711.18430.1831(10)Anim a1s1.98631.54101.58190.67351.97352.81401.98322.32531.93981.61691.84350.5598(10)2.77142.11991.66211.40882.52091.59042.92532.58672.24002.08012.19060.5179(10)1.08000.90460.83090.91960.83300.98790.56530.97430.87300.71830.86870.1458(10)0.88810.92691.02250.79040.65550.92931.05190.82670.59981.21070.89020.1834(10)Printed:25-Mar-04Page:2Testes3.10963.28263.31763.23103.45293.49673.21633.82003.75043.52753.42050.2335(10)3.74764.15173.83393.76213.75103.08794.05043.64253.75433.63963.74210.2829(10)Version4.2.2Thymus0.28570.46640.33190.30360.19260.22450.20660.36050.25490.12440.27510.0973(10)0.35040.20850.46830.31220.27570.23980.23270.25650.28740.36980.30010.0782(10)Page 619HuntingdonLifeSciencesSummarystatisticsforabsoluteorganweights(g)Printed:25-Mar-04PrincetonResearchCenterStudynumber:006130Page:1EastMillstone,NewJerseyVersion4.2.2AJ2J2endixL:RecoverySacrificeAnimalGroup/TerminalBrainKidneysLungsNo/sexSubgroupBodywt.(g)AdrenalGlandsHeartLiver-----------------------------------------------------------------------------------------------------------------------------------FemaleA n i m a 1 s1551/F1/1306.00.08132.18711.12392.51529.40601.54181552/F1/1294.80.06502.08150.92162.12607.97291.63201553/F1/1275.10.09492.29191.04332.34209.50921.57771554/F1/1335.50.07902.06111.06102.61798.92581.46871555/F1/1307.10.06532.23491.04522.45408.68271.35731556/F1/1280.20.06632.11281.06292.40218.72341.68831557/F1/1262.20.06682.06311.08102.48768.12471.54521558/F1/1285.20.06662.15930.99732.48968.21701.50541559/F1/1273.60.07582.12490.99722.21229.29571.49891560/F1/1269.40.08452.10350.94902.31459.41971.5369M e an:288.90.07462.14201.02822.39618.82771.5352Standarddeviation:22.20.01030.07640.06170.14920.57810.0901Numberofobserv.:(10)(10)(10)(10)(10)(10)(10)4551/F4/1287.60.07772.02661.01272.28727.95771.77864552/F4/1274.90.07992.14721.17312.43818.40211.33824553/F4/1314.70.08372.07171.05542.72869.62121.70444554/F4/1305.70.07312.09741.07702.32848.60181.59414555/F4/1295.90.08642.10981.06432.49859.49551.66904556/F4/1297.90.07962.17412.05782.203611.16302.01064557/F4/1279.10.07622.16461.00182.58289.46711.41774558/F4/1305.00.08682.04861.04082.38648.21531.46664559/F4/1266.80.06572.08381.04472.21376.63321.42894560/F4/1317.50.07402.05741.29352.711310.02921.6674M e an:294.50.07832.09811.18212.43798.95861.6076Standarddeviation:17.10.00650.05040.31980.19041.26450.2025Numberofobserv.:(10)(10)(10)(10)(10)(10)(10)Page 620HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:RecoverySacrificeAnimalGroup/TerminalNo/sexSubgroupBodywt.(g)1551/F1/1306.01552/F1/1294.81553/F1/1275.11554/F1/1335.51555/F1/1307.11556/F1/1280.21557/F1/1262.21558/F1/1285.21559/F1/1273.61560/F1/1269.4M e an:288.9Standarddeviation:22.2Numberofobserv.:(10)4551/F4/1287.64552/F4/1274.94553/F4/1314.74554/F4/1305.74555/F4/1295.94556/F4/1297.94557/F4/1279.14558/F4/1305.04559/F4/1266.84560/F4/1317.5M e an:294.5Standarddeviation:17.1Numberofobserv.:(10)Summarystatisticsforabsoluteorganweights(g)Studynumber:006130SpleenUterusOvariesThymusF e m a 1 eA n i m a 1 s0.05700.55200.26721.03150.08960.55130.33321.23830.11280.52790.29370.73140.11280.76430.30160.65930.05980.68820.13411.29820.10200.54780.28700.98520.06090.55470.22300.83570.08950.59310.19991.58340.10580.60440.27730.78760.13020.64690.22630.79720.09200.60310.25430.99480.02550.07570.05860.2960(10)(10)(10)(10)0.10180.54950.25230.69320.09420.51640.19020.57930.15120.63320.28021.49080.10870.76700.31500.78550.07970.50960.26521.19930.08000.74560.30260.75810.11530.58870.23610.50210.08460.52720.31220.53360.06310.55320.27260.94100.09260.59650.22880.71600.09710.59870.26550.81990.02440.09160.03990.3129(10)(10)(10)(10)Printed:25-Mar-04Page:2Version4.2.2Page 621HuntingdonLifeSciencesSummaryStatisticsfor%OrgantoBodyWeightPrinted:25-Mar-04PrincetonResearchCenterStudynumber:006130Page:1EastMillstone,NewJerseyVersion4.2.2AJ2J2endixL:RecoverySacrificeAnimalGroup/TerminalBrainHeartLiverNo/sexSubgroupBodywt.(g)AdrenalGlandsEpididymisKidneys-----------------------------------------------------------------------------------------------------------------------------------M a 1 e A n i m a 1 s1051/M1/1559.10.01080.39620.27160.35590.78912.77861052/M1/1523.70.01520.40410.32610.29590.78132.77311053/M1/1559.30.01070.41010.25250.27900.68292.41321054/M1/1509.50.01560.40370.30130.30120.73442.76231055/M1/1516.70.01060.43230.30040.30110.90143.06571056/M1/1587.20.01080.39310.25880.31510.87122.64801057/M1/1503.50.01700.46450.30430.32630.83012.71581058/M1/1608.60.01250.37990.26230.29810.79762.84931059/M1/1535.90.01120.44070.29650.35580.87022.46581060/M1/1501.90.01320.43430.31480.31580.81473.0697M e an:540.50.01280.41590.28890.31440.80732.7542Standarddeviation:36.80.00240.02610.02560.02540.06610.2157Numberofobserv.:(10)(10)(10)(10)(10)(10)(10)4051/M4/1568.50.01090.38240.2837 0.3141 0.76372.83374052/M4/1532.40.01250.39750.31320.33410.73982.60774053/M4/1565.00.01120.41350.30690.33520.72422.69824054/M4/1533.30.01310.43180.33040.33250.89742.85414055/M4/1488.00.01850.44380.36700.32540.85453.09194056/M4/1520.20.01160.45390.25140.30690.87162.70584057/M4/1566.70.01330.40770.28030.33880.89912.88774058/M4/1554.50.01130.39400.29400.26430.79822.90504059/M4/1520.70.01340.44900.32370.32671.04003.29134060/M4/1586.90.01210.39930.26890.30360.93113.1396M e an:543.60.01280.41730.30200.31810.85202.9015Standarddeviation:29.80.00220.02550.03360.02250.09770.2155Numberofobserv.:(10)(10)(10)(10)(10)(10)(10)Page 622HuntingdonLifeSciencesSummaryStatisticsfor%OrgantoBodyWeightPrinted:25-Mar-04PrincetonResearchCenterStudynumber:006130Page:2EastMillstone,NewJerseyVersion4.2.2AEEendixL:RecoverySacrificeAnimalGroup/TerminalProstateSpleenThymusNo/sexSubgroupBodywt.(g)LungsSeminalvesiclesTestes-----------------------------------------------------------------------------------------------------------------------------------M a 1 e A n i m a 1 s1051/M1/1559.10.39490.20140.35530.19320.55620.05111052/M1/1523.70.35540.25050.29430.17270.62680.08911053/M1/1559.30.35230.14150.28280.14860.59320.05931054/M1/1509.50.50650.25630.13220.18050.63420.05961055/M1/1516.70.35810.31670.38190.16120.66830.03731056/M1/1587.20.32860.21020.47920.16820.59550.03821057/M1/1503.50.42780.33970.39390.11230.63880.04101058/M1/1608.60.29620.20820.38210.16010.62770.05921059/M1/1535.90.39610.19880.36200.16290.69980.04761060/M1/1501.90.34900.26670.32220.14310.70280.0248M e an:540.50.37650.23900.33860.16030.63430.0507Standarddeviation:36.80.05880.05930.09150.02220.04660.0177Numberofobserv.(10)(10)(10)(10)(10)(10)(10)4051/M4/1568.50.34880.18830.48750.15620.65920.06164052/M4/1532.40.42650.21080.39820.17410.77980.03924053/M4/1565.00.42220.20490.29420.18100.67860.08294054/M4/1533.30.41730.23470.26420.14820.70540.05854055/M4/1488.00.41130.26590.51660.13430.76860.05654056/M4/1520.20.37990.19190.30570.17860.59360.04614057/M4/1566.70.39200.22120.51620.18560.71470.04114058/M4/1554.50.33060.17350.46650.14910.65690.04634059/M4/1520.70.38420.30770.43020.11520.72100.05524060/M4/1586.90.35410.19200.35440.20630.62010.0630M e an:543.60.38670.21910.40340.16290.68980.0550Standarddeviation:29.80.03350.04090.09460.02720.06000.0129Numberofobserv.:(10)(10)(10)(10)(10)(10)(10)Page 623HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:RecoverySacrificeAnimalGroup/TerminalNo/sexSubgroupBodywt.(g)1551/F1/11552/F1/11553/F1/11554/F1/11555/F1/11556/F1/11557/F1/11558/F1/11559/F1/11560/F1/1M e an:Standarddeviation:Numberofobserv.4551/F4/14552/F4/14553/F4/14554/F4/14555/F4/14556/F4/14557/F4/14558/F4/14559/F4/14560/F4/1M e an:Standarddeviation:Numberofobserv.:306.0294.8275.1335.5307.1280.2262.2285.2273.6269.4288.922.2(10)287.6274.9314.7305.7295.9297.9279.1305.0266.8317.5294.517.1(10)SummaryStatisticsfor%OrgantoBodyWeightStudynumber:006130AdrenalGlands0.02660.02200.03450.02350.02130.02370.02550.02340.02770.03140.02590.0042(10)0.02700.02910.02660.02390.02920.02670.02730.02850.02460.02330.02660.0021(10)BrainF e m a l e0.71470.70610.83310.61430.72770.75400.78680.75710.77660.78080.74510.0595(10)0.70470.78110.65830.68610.71300.72980.77560.67170.78100.64800.71490.0507(10)HeartAnim a1s0.36730.31260.37920.31620.34030.37930.41230.34970.36450.35230.35740.0302(10)0.35210.42670.33540.35230.35970.69080.35890.34120.39160.40740.40160.1059(10)Kidneys0.82200.72120.85130.78030.79910.85730.94870.87290.80860.85910.83200.0612(10)0.79530.88690.86700.76170.84440.73970.92540.78240.82970.85400.82860.0585(10)Printed:25-Mar-04Page:1Liver3.07392.70453.45662.66042.82733.11333.09872.88113.39763.49653.07100.3050(10)2.76693.05643.05732.81383.20903.74723.39202.69352.48623.15883.03810.3681(10)Version4.2.2Lungs0.50390.55360.57350.43780.44200.60250.58930.52780.54780.57050.53490.0576(10)0.61840.48680.54160.52150.56400.67490.50800.48090.53560.52520.54570.0601(10)Page 624HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:RecoverySacrificeAnimalGroup/TerminalNo/sexSubgroupBodywt.(g)1551/F1/1306.01552/F1/1294.81553/F1/1275.11554/F1/1335.51555/F1/1307.11556/F1/1280.21557/F1/1262.21558/F1/1285.21559/F1/1273.61560/F1/1269.4M e an:288.9Standarddeviation:22.2Numberofobserv.:(10)4551/F4/1287.64552/F4/1274.94553/F4/1314.74554/F4/1305.74555/F4/1295.94556/F4/1297.94557/F4/1279.14558/F4/1305.04559/F4/1266.84560/F4/1317.5M e an:294.5Standarddeviation:17.1Numberofobserv.:(10)SummaryStatisticsfor%OrgantoBodyWeightStudynumber:006130SpleenUterusOvariesThymusF e m a 1 eA n i m a 1 s0.01860.18040.08730.33710.03040.18700.11300.42000.04100.19190.10680.26590.03360.22780.08990.19650.01950.22410.04370.42270.03640.19550.10240.35160.02320.21160.08500.31870.03140.20800.07010.55520.03870.22090.10140.28790.04830.24010.08400.29590.03210.20870.08840.34520.00960.01970.02020.1004(10)(10)(10)(10)0.03540.19110.08770.24100.03430.18790.06920.21070.04800.20120.08900.47370.03560.25090.10300.25700.02690.17220.08960.40530.02690.25030.10160.25450.04130.21090.08460.17990.02770.17290.10240.17500.02370.20730.10220.35270.02920.18790.07210.22550.03290.20330.09010.27750.00760.02800.01250.1000(10)(10)(10)(10)Printed:25-Mar-04Page:2Version4.2.2Page 625HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:SacrificeAnimalGroup/TerminalNo/sexSubgroupBodywt.(g)1051/M1/11052/M1/11053/M1/11054/M1/11055/M1/11056/M1/11057/M1/11058/M1/11059/M1/11060/M1/1M e an:Standarddeviation:Numberofobserv.4051/M4/14052/M4/14053/M4/14054/M4/14055/M4/14056/M4/14057/M4/14058/M4/14059/M4/14060/M4/1Me an:Standarddeviation:Numberofobserv.:559.1523.7559.3509.5516.7587.2503.5608.6535.9501.9540.536.8(10)568.5532.4565.0533.3488.0520.2566.7554.5520.7586.9543.629.8(10)SummaryStatisticsfor%OrgantoBrainWeightStudynumber:006130AdrenalGlands2.72233.77042.61173.85532.45792.75983.65573.29972.53223.02773.06930.5371(10)2.85673.14242.70123.03954.17462.54543.25892.87472.97683.02973.06000.4430(10)BrainMa1e100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.00000.0000(10)100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.00000.0000(10)EpididymisAnim a1s68.555380.699361.582774.631769.502265.829065.512269.035267.284972.485069.51185.3618(10)74.201978.796974.225276.513382.706155.397968.735474.613272.092767.339572.46227.4811(10)Heart89.823973.219968.040174.597769.654480.152570.245478.458780.741972.709875.76446.6130(10)82.146584.056381.061676.999673.327267.608383.099667.078672.755776.023076.41566.1973(10)Printed:25-Mar-04Page:1Kidneys199.1783193.3286166.5315181.9048208.5289221.6325178.7070209.9165197.4678187.5866194.478316.4682(10)199.7378186.1166175.1670207.8116192.5606192.0376220.5228202.5909231.6026233.1769204.132419.2810(10)Version4.2.2Liver701.3726686.1754588.4806684.2384709.1915673.6840584.6589749.9504559.5402706.7618664.405363.7343(10)741.0848656.0439652.6028660.9119696.7722596.1459708.2403737.3478732.9799786.2770696.840655.8618(10)Page 626HuntingdonLifeSciencesSummaryStatisticsfor%OrgantoBrainWeightPrinted:25-Mar-04PrincetonResearchCenterStudynumber:006130Page:2EastMillstone,NewJerseyVersion4.2.2A22endixL:RecoverySacrificeAnimalGroup/TerminalProstateSpleenThymusNo/sexSubgroupBodywt.(g)LungsSeminalvesiclesTestes-----------------------------------------------------------------------------------------------------------------------------------M a 1 e A n i m a 1 s1051/M1/1559.199.679550.848889.674948.7585140.388312.89841052/M1/1523.787.928261.984472.808942.7404155.095722.03641053/M1/1559.385.916734.501968.973236.2285144.652314.47131054/M1/1509.5125.465563.488732.743544.7081157.081114.76011055/M1/1516.782.848373.262988.355137.2941154.58908.62291056/M1/1587.283.592653.4812121.918542.8014151.49699.72661057/M1/1503.592.102873.123084.795624.1705137.51938.83361058/M1/1608.677.965754.8069100.562242.1355165.203515.59051059/M1/1535.989.892545.105082.139236.9665158.807610.79351060/M1/1501.980.356961.411174.173132.9511161.81945.7067M e an:540.590.574957.201481.614438.8754152.665312.3440Standarddeviation:36.813.758212.079023.05876.96949.15024.6503Numberofobserv.:(10)(10)(10)(10)(10)(10)(10)4051/M4/1568.591.208949.2364127.491040.8547172.398616.11924052/M4/1532.4107.305653.0290100.174943.8002196.18669.85264053/M4/1565.0102.127649.554871.151543.7714164.122420.04714054/M4/1533.396.643554.346561.172434.3205163.356513.55624055/M4/1488.092.676159.9261116.411930.2701173.216412.73154056/M4/1520.283.698342.285467.358439.3588130.782310.15634057/M4/1566.796.152554.2586126.603545.5250175.296510.07104058/M4/1554.583.919344.0401118.406137.8422166.735311.74134059/M4/1520.785.565268.534395.804325.6533160.570612.29204060/M4/1586.988.692148.094788.760451.6621155.306215.7798M e an:543.692.798952.330697.333539.3058165.797113.2347Standarddeviation:29.87.86627.698324.83237.666116.61813.2593Numberofobserv.:(10)(10)(10)(10)(10)(10)(10)Page 627HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:RecoverySacrificeAnimalGroup/TerminalNo/sexSubgroupBodywt.(g)1551/F1/11552/F1/11553/F1/11554/F1/11555/F1/11556/F1/11557/F1/11558/F1/11559/F1/11560/F1/1Me an:Standarddeviation:Numberofobserv.4551/F4/14552/F4/14553/F4/14554/F4/14555/F4/14556/F4/14557/F4/14558/F4/14559/F4/14560/F4/1Me an:Standarddeviation:Numberofobserv.:306.0294.8275.1335.5307.1280.2262.2285.2273.6269.4288.922.2(10)287.6274.9314.7305.7295.9297.9279.1305.0266.8317.5294.517.1(10)SummaryStatisticsfor%OrgantoBrainWeightStudynumber:006130AdrenalGlands3.71733.12274.14073.83292.92183.13803.23783.08433.56724.01713.47800.4327(10)3.83403.72114.04023.48534.09523.66133.52034.23703.15293.59683.73440.3260(10)BrainFemale100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.00000.0000(10)100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.0000100.00000.0000(10)HeartA nim a 1 s51.387744.275845.521251.477446.767250.307752.396946.186346.929345.115348.03653.0277(10)49.970454.633950.943751.349350.445594.650746.281150.805450.134462.870656.208514.1922(10)Kidneys115.0016102.1379102.1860127.0147109.8036113.6927120.5758115.2966104.1084110.0309111.98488.0711(10)112.8590113.5479131.7083111.0136118.4236101.3569119.3200116.4893106.2338131.7828116.27359.7910(10)Printed:25-Mar-04Page:1Liver430.0673383.0363414.9047433.0601388.5051412.8834393.8103380.5400437.4653447.8108412.208324.5428(10)392.6626391.3050464.4109410.1173450.0663513.4539437.3603401.0202318.3223487.4696426.618856.1668(10)Version4.2.2Lungs70.495278.405068.838171.258160.732079.908274.897069.717070.539873.063971.78545.3723(10)87.762862.323082.270676.003679.107092.479765.494871.590468.571881.044076.66489.7296(10)Page 628HuntingdonLifeSciencesPrincetonResearchCenterEastMillstone,NewJerseyAppendixL:RecoverySacrificeAnimalGroup/TerminalNo/sexSubgroupBodywt.(g)1551/F1/1306.01552/F1/1294.81553/F1/1275.11554/F1/1335.51555/F1/1307.11556/F1/1280.21557/F1/1262.21558/F1/1285.21559/F1/1273.61560/F1/1269.4M e an:288.9Standarddeviation:22.2Numberofobserv.:(10)4551/F4/1287.64552/F4/1274.94553/F4/1314.74554/F4/1305.74555/F4/1295.94556/F4/1297.94557/F4/1279.14558/F4/1305.04559/F4/1266.84560/F4/1317.5M e an:294.5Standarddeviation:17.1Numberofobserv.:(10)SummaryStatisticsfor%OrgantoBrainWeightStudynumber:006130SpleenUterusOvariesThymusF e m a l e A n i m a l s2.606225.238912.217147.16294.304626.485716.007759.49084.921723.033312.814731.91245.472837.082114.633031.98782.675730.79336.000358.08764.827725.927713.583946.63012.951926.886710.809040.50704.144927.46729.257673.32934.979128.443713.050037.06536.189730.753510.758337.89874.307428.211211.913146.40721.22113.91612.862013.5217(10)(10)(10)(10)5.023227.114412.449434.20514.387124.04998.858026.97937.298430.564313.525171.96025.182636.569115.018637.45113.777624.153912.569956.84433.679734.294713.918434.86965.326627.196710.907323.19604.129725.734615.239726.04713.028126.547713.081945.15794.500828.992911.120834.80124.633428.521812.668939.15121.18264.17611.962915.1460(10)(10)(10)(10)Printed:25-Mar-04Page:2Version4.2.2Page 629

# Huntingdon Life Sciences 00

- 6130 Page 630

211

- DIPE

- S Final Report

Individual Animal Gross and

Microscopi c Observations

Preface Appendix M

Key to Abbreviations

Fibr = Fiber

Gang = Ganglia

LN = Lymph Node

Nose/Turb Sec 1 = Nose/Turbinates Section 1

Nose/Turb Sec 2 = Nose/Turbinates Section 2

Nose/Turb Sec 3 = Nose/Turbinates Section 3

Nose/Turb Sec 4 = Nose/Turbinates Section 4

SC = Spinal C ord

Submandib/Max = Submandibular/Submaxillary

Ventrl = Ventral

V- DVTC = Ventral Diverticulum

V- SM -G = Ventral Seromucinous Glands

Notes:

1. The exposure levels for each group were as follows:

Group 1

– 0 mg/m 3



# Group 2

– 2000 mg/m 3



# Group 3

– 10000 mg/m 3



# Gr oup 4

– 20000 mg/m 3



# 2. Unless otherwise specified in a histopathology note, the organ/tissue

examined was the required (routine) section.

3. “Phase Day of Death” is calculated from the first day of the indicated phase.

Table of Contents:

Individual Animal Gross and Microscopic Observations (Main and Recovery)

. 631

Individual Animal Gross and Microscopic Observations (Neuropathology)

...... 77515-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1051GROUP:1Page 63115-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1052GROUP:1Page 63215-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1053GROUP:1Page 63315-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1054GROUP:1Page 63415-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsThymusDiscolored,Red,Foci,</=0.1em,SlightSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsExamined;1correlationfound:CONGESTION,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1055GROUP:1Page 63515-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1056GROUP:1Page 63615-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1057GROUP:1Page 63715-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueThymusGrossObservations/CommentsDiscolored,Rightlobe,Red,Foci,</=0.1em,SlightSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsExamined;1correlationfound:CONGESTION,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1058GROUP:1Page 63815-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueLungs.........GrossObservations/CommentsDiscolored,Alllobes,Red,Foci,</=0.1em,SlightSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueisunremarkable.Examined;nocorrelationfoundTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1059GROUP:1Page 63915-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1060GROUP:1Page 64015-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsEsophagusNogrossobservationsontissue.Heart.Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueismissing.ANIMAL:1061GROUP:1MONONUCLEARCELLINFILTRATEWITHMYOFIBERDEGENERATION/LOSS,Minimal.Liver.........Nogrossobservationsontissue.MediastinalLN....Nogrossobservationsontissue.Lungs..Nogrossobservationsontissue.Larynx:V-SM-G.Nogrossobservationsontissue.Thyroidgland.....Nogrossobservationsontissue.MONONUCLEARCELLAGGREGATES,Minimal.SINUSES:FREEERYTHROCYTES,Minimal.Tissueisunremarkable(withcomment).-Onelobeisatelectatic.Tissueisunremarkable(withcomment).-Ventralseromucousglandsareabsent.Tissueisunremarkable(withcomment).-Kursteiner'scystsarepresentbilaterally.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainSternumFemurPage 64115-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:CecumColonDuodenumJejunumSciaticNervePancreasThoracicSCSeminalvesiclesKidneysNose/TurbSec1ProstateLumbarSCTestesMesentericLNNose/TurbSec2ParathyroidSalivaryglandsThymusEpididymisSubmandib/MaxLNNose/TurbSec3Rectum/LowColonSpleenTracheaIleumLarynx:V-DVTCNose/TurbSec4CervicalSCStomachUrinarybladderANIMAL:1061GROUP:1Page 64215-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90SEX:MalePHASE:DosingphaseANIMAL:1062GROUP:1TissueGrossObservations/CommentsHeart..Nogrossobservationsontissue.Kidneys........Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.MediastinalLN....Nogrossobservationsontissue.Lungs.........Larynx:V-DVTCDiscolored,Alllobes,Red,Foci,</=0.1em,SlightNogrossobservationsontissue.Larynx:V-SM-G....Nogrossobservationsontissue.Parathyroid......Nogrossobservationsontissue.MicroscopicObservations/CommentsMONONUCLEARCELLINFILTRATEWITHMYOFIBERDEGENERATION/LOSS,Minimal.BASOPHILICTUBULES,Minimal.CORTEX:LYMPHOIDCELLAGGREGATE(S),Minimal.MONONUCLEARCELLAGGREGATES,Minimal.SINUSES:FREEERYTHROCYTES,Minimal.Tissueisunremarkable.Examined;nocorrelationfoundSUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.Tissueisunremarkable(withcomment).-Ventralseromucousglandsareabsent.Tissueisunremarkable;one-of-pairmissing.Page 64315-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumColonDuodenumIleumJejunumMesentericLNNose/TurbSec1Nose/TurbSec2Nose/TurbSec3ProstateRectum/LowColonCervicalSCSalivaryglandsSpleenStomachThyroidglandThymusTracheaSternumEpididymisSubmandib/MaxNose/TurbSecThoracicSCLN4SeminalvesiclesUrinarybladderFemurEsophagusSciaticNervePancreasLumbarSCTestesANIMAL:1062GROUP:1Page 64415-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90SEX:MalePHASE:DosingphaseANIMAL:1063GROUP:1TissueGrossObservations/CommentsColon.........Nogrossobservationsontissue.Heart..Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.MediastinalLN....Nogrossobservationsontissue.Submandib/MaxLN...Nogrossobservationsontissue.Larynx:V-DVTC....Nogrossobservationsontissue.Larynx:V-SM-G.Nogrossobservationsontissue.Nose/TurbSec2....Nogrossobservationsontissue.Nose/TurbSec3....Nogrossobservationsontissue.Parathyroid......Nogrossobservationsontissue.MicroscopicObservations/CommentsLUMEN:NEMATODE(S),Present.MONONUCLEARCELLINFILTRATEWITHMYOFIBERDEGENERATION/LOSS,Minimal.MONONUCLEARCELLAGGREGATES,Minimal.SINUSES:FREEERYTHROCYTES,Minimal.SINUSES:FREEERYTHROCYTES,Minimal.SUBMUCOSALCYSTSWITHEOSINOPHILICCRYSTALS,Present.SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.NASOLACRIMALDUCT(S):LUMEN-EOSINOPHILICMATERIAL,UNILATERAL,Slight.NASOLACRIMALDUCT(S):LUMEN-EOSINOPHILICMATERIAL,UNILATERAL,Minimal.Tissueisunremarkable;one-of-pairmissing.Page 64515-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsRectum/LowColon...Nogrossobservationsontissue.Thymus........Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsLUMEN:NEMATODE(S),Present.CONGESTION,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsCecumJejunumNose/TurbSec1ThoracicSCSeminalvesiclesAortaDuodenumKidneysNose/TurbSec4LumbarSCTestesBrainEpididymisMesentericLNPancreasSalivaryglandsThyroid·glandSternumFemurEsophagusIleumLungsSciaticNerveProstateCervicalSCSpleenStomachTracheaUrinarybladderANIMAL:1063GROUP:1Page 64615-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsKidneys.·······Cyst,Right,Clear,Fluid,0.2-0.5emMediastinalLN.Nogrossobservationsontissue.Larynx:V-DVTC....Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsExamined;1correlationfound:CORTICALCYST,Present.CORTEX:LYMPHOIDCELLAGGREGATE(S),Minimal.CORTEX/CORTICO-MEDULLARYJUNCTION:TUBULE(S)DILATED,Minimal.-Cystisonslide28.SINUSES:FREEERYTHROCYTES,Minimal.ANIMAL:1064GROUP:1SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.Larynx:V-SM-G....Nogrossobservationsontissue.Nose/TurbSec2....Nogrossobservationsontissue.Prostate.Nogrossobservationsontissue.Rectum/LowColon...Nogrossobservationsontissue.SUBMUCOSALCYSTSWITHEOSINOPHILICCRYSTALS,Present.SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.NASOLACRIMALDUCT(S):LUMEN-EOSINOPHILICMATERIAL,BILATERAL,Slight.LYMPHOIDCELLAGGREGATE(S),Minimal.LUMEN:NEMATODE(S),Present.Page 64715-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsTestes.Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsANIMAL:1064GROUP:1SEMINIFEROUSTUBULES:DILATED,UNILATERAL,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumColonDuodenumHeartIleumJejunumSubmandib/MaxLNLungsSciaticNerveNose/TurbSec4PancreasParathyroidLumbarSCSalivaryglandsSpleenThyroidglandThymusTracheaSternumEpididymisLiverNose/TurbSec1CervicalSCStomachUrinarybladderFemurEsophagusMesentericLNNose/TurbSec3ThoracicSCSeminalvesiclesPage 64815-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsEpididymisSmall,Right,ModerateLiver..Nogrossobservationsontissue.Larynx:V-SM-G....Nogrossobservationsontissue.Rectum/LowColon...Nogrossobservationsontissue.TestesSmall,Right,SevereSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable(withcomment);one-of-pairmissing.-Rightepididymisisnotpresentedforhistopathology.MONONUCLEARCELLAGGREGATES,Minimal.Tissueisunremarkable(withcomment).-Ventralseromucousglandsareabsent.LUMEN:NEMATODE(S),Present.Tissueisunremarkable(withcomment);one-of-pairmissing.-Righttestisisnotpresentedforhistopathology.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainSternumFemurANIMAL:1065GROUP:1Page 64915-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:CecumColonDuodenumEsophagusHeartIleumJejunumKidneysMesentericLNMediastinalLNSubmandib/MaxLNLungsLarynx:V-DVTCSciaticNerveNose/TurbSec1Nose/TurbSec2Nose/TurbSec3Nose/TurbSec4PancreasProstateParathyroidCervicalSCThoracicSCLumbarSCSalivaryglandsSpleenStomachSeminalvesiclesThyroidglandThymusTracheaUrinarybladderANIMAL:1065GROUP:1Page 65015-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsKidneys.·······DilatedPelvis,Left,ModerateLiver..Nogrossobservationsontissue.Larynx:V-SM-G....Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsExamined;1correlationfound:PELVIS:DILATED,UNILATERAL,Slight.MONONUCLEARCELLAGGREGATES,Minimal.ANIMAL:1066GROUP:1SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.-Intralumenalhemorrhage.CervicalSC......Nogrossobservationsontissue.Tissueisunremarkable(withcomment).-Caudalmedullaispresent.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumColonDuodenumHeartIleumJejunumSubmandib/MaxLNLungsLarynx:V-DVTCNose/TurbSec2Nose/TurbSec3Nose/TurbSec4ParathyroidRectum/LowColonThoracicSCSternumEpididymisMesentericLNSciaticNervePancreasLumbarSCSpleenStomachSeminalvesiclesTestesThymusTracheaUrinarybladderFemurEsophagusMediastinalLNNose/TurbSec1ProstateSalivaryglandsThyroidglandPage 65115-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91SEX:MalePHASE:DosingphaseANIMAL:1067GROUP:1TissueGrossObservations/CommentsHeart..Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.MediastinalLN....Nogrossobservationsontissue.Submandib/MaxLNDiscolored/(post-fixationobservation)Enlarged/(post-fixationobservation)Larynx:V-DVTCNogrossobservationsontissue.Prostate.Nogrossobservationsontissue.Parathyroid......Nogrossobservationsontissue.MicroscopicObservations/CommentsMONONUCLEARCELLINFILTRATEWITHMYOFIBERDEGENERATION/LOSS,Minimal.MONONUCLEARCELLAGGREGATES,Minimal.SINUSES:FREEERYTHROCYTES,Minimal.Examined;1correlationfound:SINUSES:FREEERYTHROCYTES,Slight.Examined;nocorrelationfoundSUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.LYMPHOIDCELLAGGREGATE(S),Minimal.Tissueisunremarkable;one-of-pairmissing.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainSternumFemurPage 65215-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinThefollowingtissueswereunremarkablemicroscopically:CecumColonDuodenumIleumJejunumKidneysLarynx:V-SM-GSciaticNerveNose/TurbSec1Nose/TurbSec4PancreasRectum/LowColonLumbarSCSalivaryglandsSpleenTestesThyroidglandThymusSEX:MalePHASE:DosingphaseMicroscopicObservations/Commentsnormallimitsatnecropsy.EpididymisMesentericLNNose/TurbSec2CervicalSCStomachTracheaEsophagusLungsNose/TurbSec3ThoracicSCSeminalvesiclesUrinarybladderANIMAL:1067GROUP:1Page 65315-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsKidneys........Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.MediastinalLN....Nogrossobservationsontissue.Larynx:V-DVTC....Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsBASOPHILICTUBULES,Minimal.CORTEX:LYMPHOIDCELLAGGREGATE(S),Minimal.MONONUCLEARCELLAGGREGATES,Minimal.SINUSES:FREEERYTHROCYTES,Slight.ANIMAL:1068GROUP:1SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumColonDuodenumHeartIleumJejunumLungsLarynx:V-SM-GSciaticNerveNose/TurbSec3Nose/TurbSec4PancreasRectum/LowColonCervicalSCThoracicSCSternumEpididymisMesentericLNNose/TurbSec1ProstateLumbarSCSpleenStomachSeminalvesiclesTestesThymusTracheaUrinarybladderFemurLNEsophagusSubmandib/MaxNose/TurbSec2ParathyroidSalivaryglandsThyroidglandPage 65415-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsKidneys.·······DilatedPelvis/(post-fixationobservation)Liver..Nogrossobservationsontissue.MediastinalLNLungs...Larynx:V-SM-G.Nogrossobservationsontissue.Discolored,Alllobes,Red,Foci,</=0.1em,ModerateNogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsExamined;1correlationfound:PELVIS:DILATED,Minimal.BASOPHILICTUBULES,Slight.CORTEX:LYMPHOIDCELLAGGREGATE(S),Minimal.MONONUCLEARCELLAGGREGATES,Minimal.Tissueismissing.Examined;1correlationfound:HEMORRHAGE,Minimal.Tissueisunremarkable(withcomment).-Ventralseromucousglandsareabsent.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumColonDuodenumSternumEpididymisFemurEsophagusANIMAL:1069GROUP:1Page 65515-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsHeartIleumJejunumLarynx:V-DVTCSciaticNerveNose/TurbSec1Nose/TurbSec4PancreasProstateCervicalSCThoracicSCLumbarSCStomachSeminalvesiclesTestesTracheaUrinarybladderSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsMesentericLNSubmandib/MaxLNNose/TurbSec2Nose/TurbSec3ParathyroidRectum/LowColonSalivaryglandsSpleenThyroidglandThymusANIMAL:1069GROUP:1Page 65615-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsLiver..Nogrossobservationsontissue.Larynx:V-DVTC....Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsMONONUCLEARCELLAGGREGATES,Minimal.ANIMAL:1070GROUP:1SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.Larynx:V-SM-G....Nogrossobservationsontissue.SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumColonDuodenumHeartIleumJejunumMediastinalLNSubmandib/MaxLNLungsNose/TurbSec2Nose/TurbSec3Nose/TurbSec4ParathyroidRectum/LowColonCervicalSCSalivaryglandsSpleenStomachThyroidglandThymusTracheaSternumEpididymisKidneysSciaticNervePancreasThoracicSCSeminalvesiclesUrinarybladderFemurEsophagusMesentericLNNose/TurbSec1ProstateLumbarSCTestesPage 65715-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:2051GROUP:2Page 65815-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueLungs.........GrossObservations/CommentsDiscolored,Leftlobe(s),Red,Focus,0.2-0.5em,SlightSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable.Examined;nocorrelationfoundTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:2052GROUP:2Page 65915-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:2053GROUP:2Page 66015-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:2054GROUP:2Page 66115-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:2055GROUP:2Page 66215-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueLungs.........GrossObservations/CommentsDiscolored,Alllobes,Red,Foci,0.2-0.5em,SlightSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable.Examined;nocorrelationfoundTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:2056GROUP:2Page 66315-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:2057GROUP:2Page 66415-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:2058GROUP:2Page 66515-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsLungs..Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable(withcomment).-Twolobesareatelectatic.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:2059GROUP:2Page 66615-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueLungs..·······GrossObservations/CommentsDiscolored,Alllobes,Red,Foci,0.2-0.5em,SlightSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable(withcomment).Examined;nocorrelationfound-Onelobeisatelectatic.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:3051GROUP:3Page 66715-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsLungs.........Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable(withcomment).-Onelobeisatelectatic.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:3052GROUP:3Page 66815-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:3053GROUP:3Page 66915-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:3054GROUP:3Page 67015-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:3055GROUP:3Page 67115-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:3056GROUP:3Page 67215-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsLungs.........Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable(withcomment).-Onelobeisatelectatic.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:3057GROUP:3Page 67315-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsLungs.........Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable(withcomment).-Fourlobesareatelectatic.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:3058GROUP:3Page 67415-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueLungs.........GrossObservations/CommentsDiscolored,Leftlobe(s),Red,Focus,0.2-0.5em,SlightSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable.Examined;nocorrelationfoundTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:3059GROUP:3Page 67515-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsEpididymisSmall,Bilateral,severeLungs..Nogrossobservationsontissue.SeminalvesiclesSmall,Bilateral,SlightTestesSmall,Bilateral,ModerateSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueismissing.-Adiposetissueispresent.Tissueisunremarkable(withcomment).-Onelobeispartiallyatelectatic.Examined;1correlationfound:SECRETORYPRODUCT:DECREASED,Slight.Examined;1correlationfound:GERMINALEPITHELIUM:ANIMAL:3060GROUP:3DEGENERATION/ATROPHY,BILATERAL,Severe.Soft,Bilateral,SlightExamined;1correlationfound:GERMINALEPITHELIUM:DEGENERATION/ATROPHY,BILATERAL,Severe.ThymusDiscolored,Rightlobe,Red,SlightExamined;1correlationfound:CONGESTION,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.Page 67615-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4051GROUP:4Page 67715-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4052GROUP:4Page 67815-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4053GROUP:4Page 67915-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4054GROUP:4Page 68015-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueLungs.........GrossObservations/CommentsDiscolored,Alllobes,Red,Foci,</=0.1em,SlightSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueisunremarkable.Examined;nocorrelationfoundTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4055GROUP:4Page 68115-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueThymusGrossObservations/CommentsDiscolored,Rightlobe,Red,Foci,</=0.1em,SlightSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsExamined;1correlationfound:CONGESTION,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4056GROUP:4Page 68215-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4057GROUP:4Page 68315-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4058GROUP:4Page 68415-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4059GROUP:4Page 68515-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:MalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4060GROUP:4Page 68615-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90SEX:MalePHASE:DosingphaseANIMAL:4061GROUP:4TissueGrossObservations/CommentsSternum..Nogrossobservationsontissue.Kidneys........Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.Submandib/MaxLN...Nogrossobservationsontissue.Lungs.........Larynx:V-DVTCDiscolored,Alllobes,Tan,Foci,0.2-0.5em,SlightNogrossobservationsontissue.Nose/TurbSec1....Nogrossobservationsontissue.Nose/TurbSec2....Nogrossobservationsontissue.MicroscopicObservations/CommentsTissueisunremarkable(withcomment).-Marrowisslightlyhypocellular.CORTEX:PROXIMALCONVOLUTEDTUBULAREPITHELIUM-EOSINOPHILIC/HYALINEDROPLETS,Slight.BASOPHILICTUBULES,Minimal.CORTEX:LYMPHOIDCELLAGGREGATE(S),Minimal.MONONUCLEARCELLAGGREGATES,Minimal.SINUSES:FREEERYTHROCYTES,Slight.Tissueisunremarkable.Examined;nocorrelationfoundSUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.NASOLACRIMALDUCT(S):LUMEN-HEMORRHAGE(S),UNILATERAL,Slight.NASOLACRIMALDUCT(S):LUMEN-HEMORRHAGE(S),UNILATERAL,Slight.Page 68715-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsRectum/LowColon...Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsLUMEN:NEMATODE(S),Present.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainColonDuodenumEpididymisIleumJejunumMesentericLNSciaticNerveNose/TurbSec3Nose/TurbSec4ParathyroidCervicalSCThoracicSCSpleenStomachSeminalvesiclesThymusTracheaUrinarybladderFemurEsophagusMediastinalLNPancreasLumbarSCTestesCecumHeartLarynx:V-SM-GProstateSalivaryglandsThyroidglandANIMAL:4061GROUP:4Page 68815-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsKidneys..Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.Larynx:V-DVTC....Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsCORTEX:PROXIMALCONVOLUTEDTUBULAREPITHELIUM-EOSINOPHILIC/HYALINEDROPLETS,Slight.MONONUCLEARCELLAGGREGATES,Minimal.ANIMAL:4062GROUP:4SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.Nose/TurbSec1....Nogrossobservationsontissue.ODONTOPATHY,UNILATERAL,Moderate.Nose/TurbSec2....Nogrossobservationsontissue.ODONTOPATHY,BILATERAL,Slight.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumColonDuodenumHeartSubmandib/MaxNose/TurbSecCervicalSCStomachLN4IleumLungsPancreasThoracicSCSeminalvesiclesJejunumLarynx:V-SM-GProstateLumbarSCTestesSternumEpididymisMesentericLNSciaticNerveParathyroidSalivaryglandsThyroidglandFemurEsophagusMediastinalLNNose/TurbSec3Rectum/LowColonSpleenThymusPage 68915-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90SEX:MalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsTracheaUrinarybladderANIMAL:4062GROUP:4Page 69015-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsKidneys........Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.MediastinalLN....Nogrossobservationsontissue.Larynx:V-SM-G....Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsCORTEX:PROXIMALCONVOLUTEDTUBULAREPITHELIUM-EOSINOPHILIC/HYALINEDROPLETS,Slight.BASOPHILICTUBULES,Minimal.CORTEX:LYMPHOIDCELLAGGREGATE(S),Minimal.MONONUCLEARCELLAGGREGATES,Minimal.SINUSES:FREEERYTHROCYTES,Minimal.ANIMAL:4063GROUP:4SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumColonDuodenumHeartIleumJejunumLungsLarynx:V-DVTCSciaticNerveNose/TurbSec3Nose/TurbSec4PancreasSternumEpididymisMesentericLNNose/TurbSec1ProstateFemurLNEsophagusSubmandib/MaxNose/TurbSec2ParathyroidPage 69115-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsRectum/LowColonSpleenThymusCervicalSCStomachTracheaThoracicSCSeminalvesiclesUrinarybladderSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsLumbarSCTestesSalivaryglandsThyroidglandANIMAL:4063GROUP:4Page 69215-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsHeart..Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsANIMAL:4064GROUP:4MONONUCLEARCELLINFILTRATEWITHMYOFIBERDEGENERATION/LOSS,Minimal.Kidneys..Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.MediastinalLN....Nogrossobservationsontissue.Thymus........Nogrossobservationsontissue.CORTEX:PROXIMALCONVOLUTEDTUBULAREPITHELIUM-EOSINOPHILIC/HYALINEDROPLETS,Minimal.BASOPHILICTUBULES,Minimal.MONONUCLEARCELLAGGREGATES,Minimal.Tissueisunremarkable(withcomment).-Scantrepresentation.CONGESTION,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumColonDuodenumIleumJejunumMesentericLNSternumEpididymisSubmandib/MaxLNFemurEsophagusLungsPage 69315-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsLarynx:V-DVTCNose/TurbSec3Rectum/LowColonSpleenTracheaLarynx:V-SM-GNose/TurbSec4CervicalSCStomachUrinarybladderSciaticNervePancreasThoracicSCSeminalvesiclesSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsNose/TurbSec1ProstateLumbarSCTestesNose/TurbSec2ParathyroidSalivaryglandsThyroidglandANIMAL:4064GROUP:4Page 69415-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsKidneys........Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsCORTEX:PROXIMALCONVOLUTEDTUBULAREPITHELIUM-EOSINOPHILIC/HYALINEDROPLETS,Slight.BASOPHILICTUBULES,Minimal.CORTEX:LYMPHOIDCELLAGGREGATE(S),Minimal.ANIMAL:4065GROUP:4UROTHELIALHYPERPLASIA,BILATERAL,Minimal.MediastinalLN.Nogrossobservationsontissue.Larynx:V-SM-G....Nogrossobservationsontissue.SeminalvesiclesSmall,Right,SlightSINUSES:FREEERYTHROCYTES,Minimal.Tissueisunremarkable(withcomment).-Ventralseromucousglandsareabsent.Examined;1correlationfound:SECRETORYPRODUCT:DECREASED,UNILATERAL,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainSternumFemurPage 69515-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:CecumColonDuodenumHeartSubmandib/MaxLNNose/TurbSec2ParathyroidSalivaryglandsThymusIleumLungsNose/TurbSec3Rectum/LowColonSpleenTracheaJejunumLarynx:V-DVTCNose/TurbSec4CervicalSCStomachUrinarybladderEpididymisLiverSciaticNervePancreasThoracicSCTestesEsophagusMesentericLNNose/TurbSec1ProstateLumbarSCThyroidglandANIMAL:4065GROUP:4Page 69615-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsKidneys........Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.Lungs..Nogrossobservationsontissue.Larynx:V-DVTC....Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsCORTEX:PROXIMALCONVOLUTEDTUBULAREPITHELIUM-EOSINOPHILIC/HYALINEDROPLETS,Slight.BASOPHILICTUBULES,Slight.CORTEX:LYMPHOIDCELLAGGREGATE(S),Minimal.MONONUCLEARCELLAGGREGATES,Minimal.Tissueisunremarkable(withcomment).-Onelobeisatelectatic.ANIMAL:4066GROUP:4SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.TissueswithoutcommentunderGrossObservationswerewithinThefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumColonDuodenumHeartIleumSubmandib/MaxLNLarynx:V-SM-GJejunumSciaticNervenormallimitsatnecropsy.SternumEpididymisMesentericLNNose/TurbSec1FemurEsophagusMediastinalLNNose/TurbSec2Page 69715-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsNose/TurbSec3Rectum/LowColonSpleenThymusNose/TurbSec4CervicalSCStomachTracheaPancreasThoracicSCSeminalvesiclesUrinarybladderSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsProstateLumbarSCTestesParathyroidSalivaryglandsThyroidglandANIMAL:4066GROUP:4Page 69815-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsKidneys........Nogrossobservationsontissue.MediastinalLNNogrossobservationsontissue.TestesNogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsCORTEX:PROXIMALCONVOLUTEDTUBULAREPITHELIUM-EOSINOPHILIC/HYALINEDROPLETS,Slight.CORTEX:LYMPHOIDCELLAGGREGATE(S),Minimal.Tissueismissing.Tissueisunremarkable(withcomment).-Poorfixation.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumColonDuodenumHeartSubmandib/MaxLNNose/TurbSec1ProstateLumbarSCThyroidglandIleumLungsNose/TurbSec2ParathyroidSalivaryglandsThymusJejunumLarynx:V-DVTCNose/TurbSec3Rectum/LowColonSpleenTracheaSternumEpididymisLiverLarynx:V-SM-GNose/TurbSec4CervicalSCStomachUrinarybladderFemurEsophagusMesentericLNSciaticNervePancreasThoracicSCSeminalvesiclesANIMAL:4067GROUP:4Page 69915-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsKidneys........Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.Lungs.........Discolored,Alllobes,Red,Foci,0.2-0.5em,SlightLarynx:V-DVTC....Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsCORTEX:PROXIMALCONVOLUTEDTUBULAREPITHELIUM-EOSINOPHILIC/HYALINEDROPLETS,Slight.CORTEX:LYMPHOIDCELLAGGREGATE(S),Minimal.MONONUCLEARCELLAGGREGATES,Minimal.Examined;1correlationfound:HEMORRHAGE,Minimal.-Twolobesareatelectatic.ANIMAL:4068GROUP:4SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.Thymus........Nogrossobservationsontissue.CONGESTION,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainSternumFemurPage 70015-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:CecumColonDuodenumHeartSubmandib/MaxLNNose/TurbSec3Rectum/LowColonSpleenTracheaIleumLarynx:V-SM-GNose/TurbSec4CervicalSCStomachUrinarybladderJejunumSciaticNervePancreasThoracicSCSeminalvesiclesEpididymisMesentericLNNose/TurbSec1ProstateLumbarSCTestesEsophagusMediastinalLNNose/TurbSec2ParathyroidSalivaryglandsThyroidglandANIMAL:4068GROUP:4Page 70115-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsKidneys........Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.Lungs.........SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsCORTEX:PROXIMALCONVOLUTEDTUBULAREPITHELIUM-EOSINOPHILIC/HYALINEDROPLETS,Slight.BASOPHILICTUBULES,Minimal.CORTEX:LYMPHOIDCELLAGGREGATE(S),Minimal.MONONUCLEARCELLAGGREGATES,Minimal.Discolored,Alllobes,Red,Foci,</=0.1em,SlightTissueisunremarkable.Examined;nocorrelationfoundTestesNogrossobservationsontissue.Tissueisunremarkable(withcomment).-Poorlyfixed.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainSternumFemurCecumColonDuodenumEpididymisEsophagusHeartIleumJejunumMesentericLNMediastinalLNANIMAL:4069GROUP:4Page 70215-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsSubmandib/MaxLNNose/TurbSec2ParathyroidSalivaryglandsThymusLarynx:V-DVTCNose/TurbSec3Rectum/LowColonSpleenTracheaLarynx:V-SM-GNose/TurbSec4CervicalSCStomachUrinarybladderSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsSciaticNervePancreasThoracicSCSeminalvesiclesNose/TurbSec1ProstateLumbarSCThyroidglandANIMAL:4069GROUP:4Page 70315-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsKidneys.·······DilatedPelvis/(post-fixationobservation)Liver..Nogrossobservationsontissue.MediastinalLN.Nogrossobservationsontissue.Lungs.........Nogrossobservationsontissue.Larynx:V-DVTC....Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsExamined;1correlationfound:PELVIS:DILATED,UNILATERAL,Minimal.CORTEX:PROXIMALCONVOLUTEDTUBULAREPITHELIUM-EOSINOPHILIC/HYALINEDROPLETS,Slight.BASOPHILICTUBULES,Slight.CORTEX:LYMPHOIDCELLAGGREGATE(S),Minimal.MONONUCLEARCELLAGGREGATES,Minimal.SINUSES:FREEERYTHROCYTES,Minimal.Tissueisunremarkable(withcomment).-Threelobesareatelectatic.ANIMAL:4070GROUP:4SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.Larynx:V-SM-G....Nogrossobservationsontissue.SUBMUCOSALLYMPHOCYTICAGGREGATE,Slight.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainSternumFemurPage 70415-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:CecumColonDuodenumHeartSciaticNervePancreasThoracicSCSeminalvesiclesUrinarybladderIleumNose/TurbSec1ProstateLumbarSCTestesJejunumNose/TurbSec2ParathyroidSalivaryglandsThyroidglandEpididymisMesentericLNNose/TurbSec3Rectum/LowColonSpleenThymusLNEsophagusSubmandib/MaxNose/TurbSec4CervicalSCStomachTracheaANIMAL:4070GROUP:4Page 70515-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1551GROUP:1Page 70615-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1552GROUP:1Page 70715-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1553GROUP:1Page 70815-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueLungs.......·.SkinGrossObservations/CommentsDiscolored,Alllobes,Tan,Foci,<I=0.1em,SlightHairThin/Absent,foreleg,Slight/LeftHairThin/Absent,Hindleg,Slight/LeftSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsExamined;1correlationfound:VISCERALPLEURA/CAPSULE:FIBROSIS,Focal,Minimal.Examined;1correlationfound:HYPOTRICHOSIS,Minimal.Examined;1correlationfound:HYPOTRICHOSIS,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1554GROUP:1Page 70915-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1555GROUP:1Page 71015-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1556GROUP:1Page 71115-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueVaginaGrossObservations/CommentsNodule,Mucosa,Tan,Firm,0.2-0.5em/ProximalSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsExamined;1correlationfound:CYST,Present.-Sectioncompriseshairedskinhavingdeepdermalcystlinedbytransitionalepithelium.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1557GROUP:1Page 71215-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1558GROUP:1Page 71315-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1559GROUP:1Page 71415-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:1560GROUP:1Page 71515-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90SEX:FemalePHASE:DosingphaseANIMAL:1561GROUP:1TissueGrossObservations/CommentsAdrenalGlands....Nogrossobservationsontissue.Heart..Nogrossobservationsontissue.Kidneys........Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.Lungs.........Larynx:V-SM-GDiscolored,Alllobes,Red,Foci,</=0.1em,SlightNogrossobservationsontissue.Nose/TurbSec2....Nogrossobservationsontissue.MicroscopicObservations/CommentsCONGESTION,BILATERAL,Minimal.MONONUCLEARCELLINFILTRATEWITHMYOFIBERDEGENERATION/LOSS,Minimal.CORTEX:LYMPHOIDCELLAGGREGATE(S),Minimal.CORTEX/CORTICO-MEDULLARYJUNCTION:MINERALDEPOSIT(S),Minimal.MONONUCLEARCELLAGGREGATES,Minimal.Tissueisunremarkable.Examined;nocorrelationfoundGRANULOMATOUSCHONDROPATHY,Slight.SEPTALDEVIATION,Present.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AortaBrainSternumFemurCecumPage 71615-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinThefollowingtissueswereunremarkablemicroscopically:ColonDuodenumEsophagusMesentericLNMediastinalLNSubmandib/MaxLNNose/TurbSec1Nose/TurbSec3Nose/TurbSec4ParathyroidRectum/LowColonCervicalSCSalivaryglandsSpleenStomachTracheaUrinarybladderUterusSEX:FemalePHASE:DosingphaseMicroscopicObservations/Commentsnormallimitsatnecropsy.IleumLarynx:V-DVTCOvariesThoracicSCThyroidglandJejunumSciaticNervePancreasLumbarSCThymusANIMAL:1561GROUP:1Page 71715-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90SEX:FemalePHASE:DosingphaseANIMAL:1562GROUP:1TissueGrossObservations/CommentsHeart..Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.Larynx:V-SM-G.Nogrossobservationsontissue.Nose/TurbSec2....Nogrossobservationsontissue.Parathyroid......Nogrossobservationsontissue.Uterus.Nogrossobservationsontissue.MicroscopicObservations/CommentsMONONUCLEARCELLINFILTRATEWITHMYOFIBERDEGENERATION/LOSS,Minimal.MONONUCLEARCELLAGGREGATES,Minimal.Tissueisunremarkable(withcomment).-Ventralseromucousglandsareabsent.ODONTOPATHY,UNILATERAL,Minimal.-Periodontalhemorrhage,wovenboneproduction,anddisruptionofameloblastsandodontoblasts.Tissueisunremarkable;one-of-pairmissing.LUMEN:DILATED,Slight.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainSternumFemurPage 71815-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:CecumColonDuodenumJejunumLungsNose/TurbSec4ThoracicSCThyroidglandKidneysLarynx:V-DVTCOvariesLumbarThymusscMesentericLNSciaticNervePancreasSalivaryglandsTracheaEsophagusMediastinalLNNose/TurbSec1Rectum/LowColonSpleenUrinarybladderLNIleumSubmandib/MaxNose/TurbSec3CervicalSCStomachANIMAL:1562GROUP:1Page 71915-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90SEX:FemalePHASE:DosingphaseANIMAL:1563GROUP:1TissueGrossObservations/CommentsKidneys........Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.MediastinalLN....Nogrossobservationsontissue.Larynx:V-DVTC....Nogrossobservationsontissue.Larynx:V-SM-G.Nogrossobservationsontissue.Parathyroid......Nogrossobservationsontissue.Rectum/LowColon...Nogrossobservationsontissue.MicroscopicObservations/CommentsCORTEX:LYMPHOIDCELLAGGREGATE(S),Minimal.CORTEX/CORTICO-MEDULLARYJUNCTION:MINERALDEPOSIT(S),Minimal.MONONUCLEARCELLAGGREGATES,Minimal.SINUSES:FREEERYTHROCYTES,Minimal.SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.GRANULOMATOUSCHONDROPATHY,Minimal.SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.Tissueisunremarkable;one-of-pairmissing.LUMEN:NEMATODE(S),Present.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainSternumFemurPage 72015-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:CecumColonDuodenumIleumJejunumMesentericLNSciaticNerveNose/TurbSec1Nose/TurbSec2OvariesPancreasCervicalSCSalivaryglandsSpleenStomachTracheaUrinarybladderUterusEsophagusSubmandib/MaxLNNose/TurbSec3ThoracicSCThyroidglandHeartLungsNose/TurbSec4LumbarSCThymusANIMAL:1563GROUP:1Page 72115-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsMediastinalLN....Nogrossobservationsontissue.Larynx:V-SM-G.Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsSINUSES:FREEERYTHROCYTES,Minimal.ANIMAL:1564GROUP:1SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.Thymus.Nogrossobservationsontissue.MEDULLARYHYPERPLASIA,Minimal.Uterus.Nogrossobservationsontissue.LUMEN:DILATED,Slight.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumIleumSubmandib/MaxLNNose/TurbSec2ParathyroidSalivaryglandsUrinarybladderColonJejunumLungsNose/TurbSec3Rectum/LowColonSpleenDuodenumKidneysLarynx:V-DVTCNose/TurbSec4CervicalSCStomachSternumEsophagusLiverSciaticNerveOvariesThoracicSCThyroidglandFemurHeartMesentericLNNose/TurbSec1PancreasLumbarSCTracheaPage 72215-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsAdrenalGlands....Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.Lungs.........Nogrossobservationsontissue.Parathyroid......Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable(withcomment).-Oneglandhasscantrepresentationofmedulla.MONONUCLEARCELLAGGREGATES,Minimal.Tissueisunremarkable(withcomment).-Onelobeisatelectatic.Tissueisunremarkable;one-of-pairmissing.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AortaColonJejunumLarynx:V-DVTCNose/TurbSec3CervicalSCStomachUterusBrainDuodenumKidneysLarynx:V-SM-GNose/TurbSec4ThoracicSCThyroidglandSternumEsophagusMesentericLNSciaticNerveOvariesLumbarSCThymusFemurCecumHeartIleumMediastinalLNSubmandib/MaxLNNose/TurbSec1Nose/TurbSec2PancreasRectum/LowColonSalivaryglandsSpleenTracheaUrinarybladderANIMAL:1565GROUP:1Page 72315-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91SEX:FemalePHASE:DosingphaseANIMAL:1566GROUP:1TissueGrossObservations/CommentsKidneys........Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.Larynx:V-SM-G....Nogrossobservationsontissue.Nose/TurbSec1....Nogrossobservationsontissue.Nose/TurbSec2....Nogrossobservationsontissue.Parathyroid......Nogrossobservationsontissue.SkinHairThin/Absent,Thoracic,ModerateMicroscopicObservations/CommentsCORTEX/CORTICO-MEDULLARYJUNCTION:MINERALDEPOSIT(S),Minimal.MONONUCLEARCELLAGGREGATES,Minimal.SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.ODONTOPATHY,BILATERAL,Minimal.ODONTOPATHY,BILATERAL,Slight.Tissueisunremarkable;one-of-pairmissing.Examined;1correlationfound:HYPOTRICHOSIS,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainSternumFemurPage 72415-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsCecumColonDuodenumIleumJejunumMesentericLNLungsLarynx:V-DVTCSciaticNerveOvariesPancreasRectum/LowColonLumbarSCSalivaryglandsSpleenThymusTracheaUrinarybladderSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsEsophagusHeartMediastinalLNSubmandib/MaxLNNose/TurbSec3Nose/TurbSec4CervicalSCThoracicSCStomachThyroidglandUterusANIMAL:1566GROUP:1Page 72515-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsColon.........Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.Larynx:V-SM-G....Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsLUMEN:NEMATODE(S),Present.MONONUCLEARCELLAGGREGATES,Minimal.GRANULOMATOUSCHONDROPATHY,Slight.ANIMAL:1567GROUP:lSUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.Parathyroid......Nogrossobservationsontissue.SkinHairThin/Absent,Hindpaws,ModerateHairThin/Absent,Forepaws,SevereTissueisunremarkable;one-of-pairmissing.Examined;1correlationfound:HYPOTRICHOSIS,Minimal.Examined;1correlationfound:HYPOTRICHOSIS,Minimal.EPITHELIUM:SQUAMOUSCELLHYPERPLASIA,Minimal.EPITHELIUM:HYPERKERATOSIS,Minimal.-Slide28:skinfromhindpaws;slide29:skinfromforepaws.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainSternumFemurPage 72615-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:CecumDuodenumEsophagusJejunumKidneysMesentericLNLungsLarynx:V-DVTCSciaticNerveNose/TurbSec3Nose/TurbSec4OvariesCervicalSCThoracicSCLumbarSCStomachThyroidglandThymusUterusHeartMediastinalLNNose/TurbSec1PancreasSalivaryglandsTracheaIleumSubmandib/MaxLNNose/TurbSec2Rectum/LowColonSpleenUrinarybladderANIMAL:1567GROUP:1Page 72715-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91SEX:FemalePHASE:DosingphaseANIMAL:1568GROUP:1TissueGrossObservations/CommentsKidneys........Nogrossobservationsontissue.Larynx:V-DVTC....Nogrossobservationsontissue.Larynx:V-SM-G....Nogrossobservationsontissue.Parathyroid......Nogrossobservationsontissue.Uterus.Nogrossobservationsontissue.MicroscopicObservations/CommentsCORTEX/CORTICO-MEDULLARYJUNCTION:MINERALDEPOSIT(S),Minimal.SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.SUBMUCOSALCYSTSWITHEOSINOPHILICCRYSTALS,Present.Tissueisunremarkable(withcomment).-Ventralseromucousglandsareabsent.Tissueisunremarkable;one-of-pairmissing.CERVIX:LYMPHANGIECTASIA,Present.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumIleumSubmandib/MaxLNColonJejunumLungsDuodenumLiverSciaticNerveSternumEsophagusMesentericLNNose/TurbSec1FemurHeartMediastinalLNNose/TurbSec2Page 72815-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsNose/TurbSec3CervicalSCStomachNose/TurbSec4ThoracicSCThyroidglandOvariesLumbarSCThymusSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsPancreasSalivaryglandsTracheaRectum/LowColonSpleenUrinarybladderANIMAL:1568GROUP:1Page 72915-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsKidneys........Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsANIMAL:1569GROUP:1CORTEX/CORTICO-MEDULLARYJUNCTION:MINERALDEPOSIT(S),Minimal.Larynx:V-DVTC....Nogrossobservationsontissue.Larynx:V-SM-G....Nogrossobservationsontissue.SUBMUCOSALCYSTSWITHEOSINOPHILICCRYSTALS,Present.Tissueisunremarkable(withcomment).-Ventralseromucousglandsareabsent.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumColonDuodenumIleumJejunumLiverSubmandib/MaxLNLungsSciaticNerveNose/TurbSec3Nose/TurbSec4OvariesRectum/LowColonCervicalSCThoracicSCSpleenStomachThyroidglandUrinarybladderUterusSternumEsophagusMesentericLNNose/TurbSec1PancreasLumbarSCThymusFemurHeartMediastinalLNNose/TurbSec2ParathyroidSalivaryglandsTracheaPage 73015-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsKidneys........Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsANIMAL:1570GROUP:1CORTEX/CORTICO-MEDULLARYJUNCTION:MINERALDEPOSIT(S),Slight.MediastinalLN....Nogrossobservationsontissue.Lungs..Nogrossobservationsontissue.Larynx:V-DVTC....Nogrossobservationsontissue.SINUSES:FREEERYTHROCYTES,Minimal.Tissueisunremarkable(withcomment).-Onelobeisatelectatic.SUBMUCOSALCYSTSWITHEOSINOPHILICCRYSTALS,Present.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumColonDuodenumIleumJejunumLiverLarynx:V-SM-GSciaticNerveNose/TurbSec1Nose/TurbSec4OvariesPancreasCervicalSCThoracicSCLumbarSCStomachThyroidglandThymusUterusSternumEsophagusMesentericLNNose/TurbSec2ParathyroidSalivaryglandsTracheaFemurHeartSubmandib/MaxLNNose/TurbSec3Rectum/LowColonSpleenUrinarybladderPage 73115-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90SEX:FemalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:2551GROUP:2Page 73215-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsLungs..Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable(withcomment).-Threelobesareatelectatic.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:2552GROUP:2Page 73315-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsLungs..Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable(withcomment).-Onelobeisatelectatic.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:2553GROUP:2Page 73415-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueSkinGrossObservations/CommentsHairThin/Absent,Abdominal,Area,6.0-6.9em,ModerateSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsExamined;1correlationfound:HYPOTRICHOSIS,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:2554GROUP:2Page 73515-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90SEX:FemalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:2555GROUP:2Page 73615-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91SEX:FemalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:2556GROUP:2Page 73715-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsLungs..Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable(withcomment).-Onelobeisatelectatic.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:2557GROUP:2Page 73815-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsLungs.........Discolored,Alllobes,Pale,SlightSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable(withcomment).Examined;nocorrelationfound-Threelobesareatelectatic.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:2558GROUP:2Page 73915-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91SEX:FemalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:2559GROUP:2Page 74015-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91SEX:FemalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:2560GROUP:2Page 74115-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90SEX:FemalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:3551GROUP:3Page 74215-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsSkinHairThin/Absent,Forepaws,SevereHairThin/Absent,Hindpaws,ModerateSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsExamined;1correlationfound:HYPOTRICHOSIS,Minimal.Examined;1correlationfound:HYPOTRICHOSIS,Minimal.EPITHELIUM:HYPERKERATOSIS,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:3552GROUP:3Page 74315-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90SEX:FemalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:3553GROUP:3Page 74415-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsLungs.........Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable(withcomment).-Onelobeisatelectatic.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:3554GROUP:3Page 74515-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90SEX:FemalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:3555GROUP:3Page 74615-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91SEX:FemalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:3556GROUP:3Page 74715-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsLungs.........Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable(withcomment).-Onelobeisatelectatic.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:3557GROUP:3Page 74815-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueLungs.........GrossObservations/CommentsDiscolored,Leftdiaphragmaticlobe,Tan,Foci,</=0.1em,SlightSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable(withcomment).Examined;nocorrelationfound-Onelobeisatelectatic.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:3558GROUP:3Page 74915-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsLungs..Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable(withcomment).-Onelobeisatelectatic.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:3559GROUP:3Page 75015-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsANIMAL:3560GROUP:3Page 75115-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4551GROUP:4Page 75215-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4552GROUP:4Page 75315-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4553GROUP:4Page 75415-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4554GROUP:4Page 75515-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4555GROUP:4Page 75615-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueLungs....GrossObservations/CommentsDiscolored,Alllobes,Red,Foci,0.2-0.5em,SlightSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueisunremarkable.Examined;nocorrelationfoundTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4556GROUP:4Page 75715-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4557GROUP:4Page 75815-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4558GROUP:4Page 75915-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4559GROUP:4Page 76015-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:32TissueGrossObservations/CommentsSEX:FemalePHASE:RecoveryphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:Notissuesexamined.ANIMAL:4560GROUP:4Page 76115-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsLarynx:V-DVTC.Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsANIMAL:4561GROUP:4SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.Larynx:V-SM-G....Nogrossobservationsontissue.Parathyroid......Nogrossobservationsontissue.SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.Tissueisunremarkable;one-of-pairmissing.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumColonDuodenumIleumJejunumKidneysMediastinalLNSubmandib/MaxLNLungsNose/TurbSec2Nose/TurbSec3Nose/TurbSec4Rectum/LowColonCervicalSCThoracicSCSpleenStomachThyroidglandUrinarybladderUterusSternumEsophagusLiverSciaticNerveOvariesLumbarscThymusFemurHeartMesentericLNNose/TurbSec1PancreasSalivaryglandsTracheaPage 76215-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsKidneys..Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsBASOPHILICTUBULES,Minimal.CORTEX:LYMPHOIDCELLAGGREGATE(S),Minimal.ANIMAL:4562GROUP:4CORTEX/CORTICO-MEDULLARYJUNCTION:MINERALDEPOSIT(S),Minimal.Liver..Nogrossobservationsontissue.MONONUCLEARCELLAGGREGATES,Minimal.Larynx:V-SM-G....Nogrossobservationsontissue.Tissueisunremarkable(withcomment).-Ventralseromucousglandsareabsent.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumIleumLungsNose/TurbSec3Rectum/LowColonSpleenUrinarybladderColonJejunumLarynx:V-DVTCNose/TurbSec4CervicalSCStomachUterusDuodenumMesentericLNSciaticNerveOvariesThoracicSCThyroidglandSternumEsophagusMediastinalLNNose/TurbSec1PancreasLumbarSCThymusFemurHeartSubmandib/MaxLNNose/TurbSec2ParathyroidSalivaryglandsTracheaPage 76315-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsKidneys........Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.Lungs..Nogrossobservationsontissue.Larynx:V-DVTC....Nogrossobservationsontissue.Larynx:V-SM-G....Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsBASOPHILICTUBULES,Minimal.MONONUCLEARCELLAGGREGATES,Minimal.ALVEOLAR/INTRAALVEOLARMACROPHAGES,Minimal.-Twolobesareatelectatic.SUBMUCOSALCYSTSWITHEOSINOPHILICCRYSTALS,Present.ANIMAL:4563GROUP:4SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.Nose/TurbSec1....Nogrossobservationsontissue.Nose/TurbSec2....Nogrossobservationsontissue.Ovaries..Nogrossobservationsontissue.Parathyroid......Nogrossobservationsontissue.ODONTOPATHY,BILATERAL,Minimal.SEPTALDEVIATION,Present.SEPTALDEVIATION,Present.ODONTOPATHY,BILATERAL,Moderate.MINERALIZATION,Minimal.Tissueisunremarkable;one-of-pairmissing.Page 76415-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90SEX:FemalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsRectum/LowColon...Nogrossobservationsontissue.LUMEN:NEMATODE(S),Present.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainSternumFemurCecumColonDuodenumEsophagusHeartIleumJejunumMesentericLNMediastinalLNSubmandib/MaxLNSciaticNerveNose/TurbSec3Nose/TurbSec4PancreasCervicalSCThoracicSCLumbarSCSalivaryglandsSpleenStomachThyroidglandThymusTracheaUrinarybladderUterusANIMAL:4563GROUP:4Page 76515-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsLiver..Nogrossobservationsontissue.MediastinalLN.Nogrossobservationsontissue.Larynx:V-SM-G.Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsMONONUCLEARCELLAGGREGATES,Minimal.SINUSES:FREEERYTHROCYTES,Minimal.ANIMAL:4564GROUP:4SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumIleumLungsNose/TurbSec3Rectum/LowColonSpleenUrinarybladderColonJejunumLarynx:V-DVTCNose/TurbSec4CervicalSCStomachUterusDuodenumKidneysSciaticNerveOvariesThoracicSCThyroidglandSternumEsophagusMesentericLNNose/TurbSec1PancreasLumbarSCThymusFemurHeartSubmandib/MaxLNNose/TurbSec2ParathyroidSalivaryglandsTracheaPage 76615-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90SEX:FemalePHASE:DosingphaseANIMAL:4565GROUP:4TissueGrossObservations/CommentsKidneys........Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.Larynx:V-DVTC....Nogrossobservationsontissue.Larynx:V-SM-G....Nogrossobservationsontissue.Nose/TurbSec1....Nogrossobservationsontissue.Nose/TurbSec2....Nogrossobservationsontissue.Parathyroid......Nogrossobservationsontissue.Stomach..Nogrossobservationsontissue.MicroscopicObservations/CommentsCORTEX/CORTICO-MEDULLARYJUNCTION:MINERALDEPOSIT(S),Minimal.MONONUCLEARCELLAGGREGATES,Minimal.SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.GRANULOMATOUSCHONDROPATHY,Minimal.SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.NASOLACRIMALDUCT(S):LUMEN-HEMORRHAGE(S),UNILATERAL,Minimal.NASOLACRIMALDUCT(S):LUMEN-HEMORRHAGE(S),UNILATERAL,Minimal.Tissueisunremarkable;one-of-pairmissing.GLANDULARECTASIA,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainSternumFemurPage 76715-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:90TissueGrossObservations/CommentsSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:CecumColonDuodenumIleumJejunumMesentericLNLungsSciaticNerveNose/TurbSec3PancreasRectum/LowColonCervicalSCSalivaryglandsSpleenThyroidglandUrinarybladderUterusEsophagusMediastinalLNNose/TurbSec4ThoracicSCThymusHeartSubmandib/MaxLNOvariesLumbarSCTracheaANIMAL:4565GROUP:4Page 76815-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsLiver..Nogrossobservationsontissue.Larynx:V-DVTC....Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsMONONUCLEARCELLAGGREGATES,Minimal.ANIMAL:4566GROUP:4SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.Larynx:V-SM-G.Nogrossobservationsontissue.Tissueisunremarkable(withcomment).-Ventralseromucousglandsareabsent.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumIleumSubmandib/MaxLNNose/TurbSec3Rectum/LowColonSpleenUrinarybladderColonJejunumLungsNose/TurbSec4CervicalSCStomachUterusDuodenumKidneysSciaticNerveOvariesThoracicSCThyroidglandSternumEsophagusMesentericLNNose/TurbSec1PancreasLumbarSCThymusFemurHeartMediastinalLNNose/TurbSec2ParathyroidSalivaryglandsTracheaPage 76915-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsLiver..Nogrossobservationsontissue.MediastinalLN.Nogrossobservationsontissue.Larynx:V-SM-G....Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsMONONUCLEARCELLAGGREGATES,Minimal.SINUSES:FREEERYTHROCYTES,Minimal.ANIMAL:4567GROUP:4SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumColonDuodenumIleumJejunumKidneysLungsLarynx:V-DVTCSciaticNerveNose/TurbSec3Nose/TurbSec4OvariesRectum/LowColonCervicalSCThoracicSCSpleenStomachThyroidglandUrinarybladderUterusSternumEsophagusMesentericLNNose/TurbSec1PancreasLumbarSCThymusFemurHeartSubmandib/MaxNose/TurbSecLN2ParathyroidSalivaryglandsTracheaPage 77015-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91SEX:FemalePHASE:DosingphaseANIMAL:4568GROUP:4TissueGrossObservations/CommentsKidneys..Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.MediastinalLN.Nogrossobservationsontissue.Lungs.........Discolored,Alllobes,Tan,Foci,</=0.1em,SlightLarynx:V-DVTC....Nogrossobservationsontissue.Larynx:V-SM-G....Nogrossobservationsontissue.Parathyroid......Nogrossobservationsontissue.Rectum/LowColon...Nogrossobservationsontissue.MicroscopicObservations/CommentsCORTEX/CORTICO-MEDULLARYJUNCTION:MINERALDEPOSIT(S),Minimal.MONONUCLEARCELLAGGREGATES,Minimal.SINUSES:FREEERYTHROCYTES,Slight.Examined;1correlationfound:ALVEOLAR/INTRAALVEOLARMACROPHAGES,Minimal.SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.GRANULOMATOUSCHONDROPATHY,Minimal.SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.Tissueisunremarkable;one-of-pairmissing.LUMEN:NEMATODE(S),Present.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainSternumFemurPage 77115-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinThefollowingtissueswereunremarkablemicroscopically:CecumColonDuodenumIleumJejunumMesentericLNNose/TurbSec1Nose/TurbSec2Nose/TurbSec3PancreasCervicalSCThoracicSCSpleenStomachThyroidglandUrinarybladderUterusSEX:FemalePHASE:DosingphaseMicroscopicObservations/Commentsnormallimitsatnecropsy.EsophagusSubmandib/MaxLNNose/TurbSec4LumbarSCThymusHeartSciaticNerveOvariesSalivaryglandsTracheaANIMAL:4568GROUP:4Page 77215-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91SEX:FemalePHASE:DosingphaseANIMAL:4569GROUP:4TissueGrossObservations/CommentsKidneys........Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.Larynx:V-SM-G.Nogrossobservationsontissue.Parathyroid......Nogrossobservationsontissue.MicroscopicObservations/CommentsCORTEX/CORTICO-MEDULLARYJUNCTION:MINERALDEPOSIT(S),Minimal.MONONUCLEARCELLAGGREGATES,Minimal.SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.Tissueisunremarkable;one-of-pairmissing.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumColonDuodenumIleumJejunumMesentericLNLungsLarynx:V-DVTCSciaticNerveNose/TurbSec3Nose/TurbSec4OvariesCervicalSCThoracicscLumbarSCStomachThyroidglandThymusUterusSternumEsophagusMediastinalLNNose/TurbSec1PancreasSalivaryglandsTracheaFemurHeartSubmandib/MaxLNNose/TurbSec2Rectum/LowColonSpleenUrinarybladderPage 77315-Dec-11;17:46HuntingdonLifeSciences,Inc.006130MainStudyGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:91TissueGrossObservations/CommentsKidneys..Nogrossobservationsontissue.Liver..Nogrossobservationsontissue.Larynx:V-SM-G....Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsCORTEX:LYMPHOIDCELLAGGREGATE(S),Minimal.MONONUCLEARCELLAGGREGATES,Minimal.GRANULOMATOUSCHONDROPATHY,Minimal.ANIMAL:4570GROUP:4SUBMUCOSALLYMPHOCYTICAGGREGATE,Minimal.Uterus.Nogrossobservationsontissue.LUMEN:DILATED,Slight.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:AdrenalGlandsAortaBrainCecumColonDuodenumIleumJejunumMesentericLNLungsLarynx:V-DVTCSciaticNerveNose/TurbSec3Nose/TurbSec4OvariesRectum/LowColonCervicalSCThoracicSCSpleenStomachThyroidglandUrinarybladderSternumEsophagusMediastinalLNNose/TurbSec1PancreasLumbarSCThymusFemurLNHeartSubmandib/MaxNose/TurbSec2ParathyroidSalivaryglandsTracheaPage 77415-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93TissueGrossObservations/CommentsKidneys.·······SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsDiscolored,Bilateral,Tan,Foci,0.2-0.5em,ModerateExamined;1correlationfound:DILATEDTUBULES,BILATERAL,Slight.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:BrainEyesLungsSuralNerveTrigeminalGangDorsalRootGangOpticnerveCervicalSCThoracicSCSciaticNerveDorsalRootFibrLumbarSCTibialNerveVentrlRootFibrTracheaANIMAL:1071GROUP:1Page 77515-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:BrainEyesLungsSuralNerveTrigeminalGangDorsalRootGangOpticnerveCervicalSCThoracicSCSciaticNerveDorsalRootFibrLumbarSCTibialNerveVentrlRootFibrTracheaANIMAL:1072GROUP:1Page 77615-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93SEX:MalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:BrainEyesLungsSciaticNerveTibialNerveSuralNerveTrigeminalGangDorsalRootGangDorsalRootFibrVentrlRootFibrOpticnerveCervicalSCThoracicSCLumbarSCTracheaANIMAL:1073GROUP:1Page 77715-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94SEX:MalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsLungs.........Tissueisunremarkable.Examined;nocorrelationfoundDiscolored,Alllobes/(post-fixationobservation)TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:BrainEyesSciaticNerveTibialNerveSuralNerveTrigeminalGangDorsalRootGangDorsalRootFibrVentrlRootFibrOpticnerveCervicalSCThoracicSCLumbarSCTracheaANIMAL:1074GROUP:1Page 77815-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94TissueGrossObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinThefollowingtissueswereunremarkablemicroscopically:BrainEyesLungsSuralNerveTrigeminalGangDorsalRootGangOpticnerveCervicalSCThoracicSCSEX:MalePHASE:DosingphaseMicroscopicObservations/Commentsnormallimitsatnecropsy.SciaticNerveDorsalRootFibrLumbarSCTibialNerveVentrlRootFibrTracheaANIMAL:1075GROUP:1Page 77915-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsTracheaANIMAL:2061GROUP:2Page 78015-Dec-ll;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsTracheaANIMAL:2062GROUP:2Page 78115-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsTracheaANIMAL:2063GROUP:2Page 78215-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsTracheaANIMAL:2064GROUP:2Page 78315-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94TissueGrossObservations/CommentsLungs..Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsHEMORRHAGE(S),Multifocal,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:TracheaANIMAL:2065GROUP:2Page 78415-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsTracheaANIMAL:3061GROUP:3Page 78515-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93TissueGrossObservations/CommentsLungs..Nogrossobservationsontissue.SEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsALVEOLARMACROPHAGES,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:TracheaANIMAL:3062GROUP:3Page 78615-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsTracheaANIMAL:3063GROUP:3Page 78715-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsTracheaANIMAL:3064GROUP:3Page 78815-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsTracheaANIMAL:3065GROUP:3Page 78915-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93TissueGrossObservations/CommentsSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:BrainSuralNerveOpticnerveEyesTrigeminalGangCervicalSCLungsDorsalRootGangThoracicSCSciaticNerveDorsalRootFibrLumbarSCTibialNerveVentrlRootFibrTracheaANIMAL:4071GROUP:4Page 79015-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93SEX:MalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsLungs..Nogrossobservationsontissue.HEMORRHAGE(S),Focal,Minimal.ThymusDiscolored,Red,Foci,</=0.1em,SlightExamined;1correlationfound:CONGESTION,Slight.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:BrainEyesSciaticNerveTibialNerveSuralNerveTrigeminalGangDorsalRootGangDorsalRootFibrVentrlRootFibrOpticnerveCervicalSCThoracicSCLumbarSCTracheaANIMAL:4072GROUP:4Page 79115-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94SEX:MalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:BrainEyesLungsSciaticNerveTibialNerveSuralNerveTrigeminalGangDorsalRootGangDorsalRootFibrVentrlRootFibrOpticnerveCervicalSCThoracicSCLumbarSCTracheaANIMAL:4073GROUP:4Page 79215-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94TissueKidneys. .······SkinGrossObservations/CommentsDiscolored,Bilateral,Tan,Foci,</=0.1em,ModerateHairThin/Absent,Slight/LeftlateralScab,Cervical,Tan,1.0-1.5em/WithhairlossSEX:MalePHASE:DosingphaseMicroscopicObservations/CommentsExamined;nocorrelationfoundPROXIMALTUBULAREPITHELIUM:EOSINOPHILICCRYSTALS,Slight.Examined;1correlationfound:HYPOTRICHOSIS,Slight.Examined;1correlationfound:EPITHELIUM:ULCER(S),Marked.SURFACEBACTERIA,Present.DERMALGRANULATIONTISSUE,Moderate.NEUTROPHILICINFILTRATE,Moderate.-Slide"0"hascervicalskin.Slide"P"hasleftlateralskin.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:BrainEyesLungsSciaticNerveTibialNerveANIMAL:4074GROUP:4Page 79315-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94SEX:MalePHASE:DosingphaseTissueSuralNerveOpticnerveGrossObservations/CommentsMicroscopicObservations/CommentsTrigeminalGangCervicalSCDorsalRootGangDorsalRootFibrVentrlRootFibrThoracicSCLumbarSCTracheaANIMAL:4074GROUP:4Page 79415-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94SEX:MalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsLungs.........Nogrossobservationsontissue.HEMORRHAGE(S),Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:BrainEyesSciaticNerveTibialNerveSuralNerveTrigeminalGangDorsalRootGangDorsalRootFibrVentrlRootFibrOpticnerveCervicalSCThoracicSCLumbarSCTracheaANIMAL:4075GROUP:4Page 79515-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93TissueGrossObservations/CommentsSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:BrainSuralNerveOpticnerveEyesTrigeminalGangCervicalSCLungsDorsalRootGangThoracicSCSciaticNerveDorsalRootFibrLumbarSCTibialNerveVentrlRootFibrTracheaANIMAL:1571GROUP:1Page 79615-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93TissueGrossObservations/CommentsEyes.........Nogrossobservationsontissue.SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsANIMAL:1572GROUP:1CILIARYBODY:MONONUCLEARCELLINFILTRATE,Minimal.SuralNerve..Nogrossobservationsontissue.Tissueismissing.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:BrainLungsSciaticNerveTibialNerveDorsalRootGangDorsalRootFibrVentr1RootFibrOpticnerveThoracicSCLumbarSCTracheaTrigeminalGangCervicalSCPage 79715-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94TissueGrossObservations/CommentsSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:BrainEyesLungsSuralNerveTrigeminalGangDorsalRootGangOpticnerveCervicalSCThoracicSCSciaticNerveDorsalRootFibrLumbarSCTibialNerveVentrlRootFibrTracheaANIMAL:1573GROUP:1Page 79815-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94SEX:FemalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsSuralNerve..Nogrossobservationsontissue.Tissueisunremarkable(withcomment).-Thelongitudinalsectionismissing.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:BrainEyesLungsSciaticNerveTibialNerveTrigeminalGangDorsalRootGangDorsalRootFibrVentrlRootFibrOpticnerveCervicalSCThoracicSCLumbarSCTracheaANIMAL:1574GROUP:1Page 79915-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94SEX:FemalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:BrainEyesLungsSciaticNerveTibialNerveSuralNerveTrigeminalGangDorsalRootGangDorsalRootFibrVentrlRootFibrOpticnerveCervicalSCThoracicSCLumbarSCTracheaANIMAL:1575GROUP:1Page 80015-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93SEX:FemalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsTracheaANIMAL:2561GROUP:2Page 80115-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93TissueGrossObservations/CommentsSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsTracheaANIMAL:2562GROUP:2Page 80215-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93SEX:FemalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsTracheaANIMAL:2563GROUP:2Page 80315-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94SEX:FemalePHASE:DosingphaseTissueLungs. . . . . . . . .GrossObservations/CommentsDiscolored,Alllobes/(post-fixationobservation)MicroscopicObservations/CommentsTissueisunremarkable.Examined;nocorrelationfoundTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:TracheaANIMAL:2564GROUP:2Page 80415-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94SEX:FemalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsTracheaANIMAL:2565GROUP:2Page 80515-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93SEX:FemalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsTracheaANIMAL:3561GROUP:3Page 80615-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93TissueLungs..·······GrossObservations/CommentsDiscolored,Alllobes/(post-fixationobservation)SEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueisunremarkable.Examined;nocorrelationfoundTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:TracheaANIMAL:3562GROUP:3Page 80715-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94TissueGrossObservations/CommentsSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsTracheaANIMAL:3563GROUP:3Page 80815-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94TissueLungs.........GrossObservations/CommentsDiscolored,Rightdiaphragmaticlobe,Tan,Foci,<I=0.1em,SlightSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsExamined;nocorrelationfoundHEMORRHAGE(S),Focal,Minimal.TissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:TracheaANIMAL:3564GROUP:3Page 80915-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94TissueGrossObservations/CommentsSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:LungsTracheaANIMAL:3565GROUP:3Page 81015-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93TissueGrossObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinThefollowingtissueswereunremarkablemicroscopically:BrainEyesLungsSuralNerveTrigeminalGangDorsalRootGangOpticnerveCervicalSCThoracicSCSEX:FemalePHASE:DosingphaseMicroscopicObservations/Commentsnormallimitsatnecropsy.SciaticNerveDorsalRootFibrLumbarSCTibialNerveVentrlRootFibrTracheaANIMAL:4571GROUP:4Page 81115-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93TissueGrossObservations/CommentsSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:BrainEyesLungsSuralNerveTrigeminalGangDorsalRootGangOpticnerveCervicalSCThoracicSCSciaticNerveDorsalRootFibrLumbarSCTibialNerveVentrlRootFibrTracheaANIMAL:4572GROUP:4Page 81215-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:93SEX:FemalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:BrainEyesLungsSciaticNerveTibialNerveSuralNerveTrigeminalGangDorsalRootGangDorsalRootFibrVentrlRootFibrOpticnerveCervicalSCThoracicSCLumbarSCTracheaANIMAL:4573GROUP:4Page 81315-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94TissueGrossObservations/CommentsSEX:FemalePHASE:DosingphaseMicroscopicObservations/CommentsTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:BrainSuralNerveOpticnerveEyesTrigeminalGangCervicalSCLungsDorsalRootGangThoracicSCSciaticNerveDorsalRootFibrLumbarSCTibialNerveVentrlRootFibrTracheaANIMAL:4574GROUP:4Page 81415-Dec-11;19:15HuntingdonLifeSciences,Inc.006130NNeurotoxGasolineDIPEVaporCondensate:A13-WeekWhole-BodyInhalationToxicityStudyinRatswithNeurotoxicityAssessmentsand4-WeekInVivoGenotoxicityandImmunotoxicityAssessmentsIndividualAnimalGrossandMicroscopicObservationsSTATUS:FinalphasesacrificePHASEDAYOFDEATH:94SEX:FemalePHASE:DosingphaseTissueGrossObservations/CommentsMicroscopicObservations/CommentsLungs.........Tissueisunremarkable.Examined;nocorrelationfoundDiscolored,Rightdiaphragmaticlobe,Tan,Focus,0.2-0.5em,SlightTissueswithoutcommentunderGrossObservationswerewithinnormallimitsatnecropsy.Thefollowingtissueswereunremarkablemicroscopically:BrainEyesSciaticNerveTibialNerveSuralNerveTrigeminalGangDorsalRootGangDorsalRootFibrVentrlRootFibrOpticnerveCervicalSCThoracicSCLumbarSCTracheaANIMAL:4575GROUP:4Page 815