STUDY NO. 00-6130

SPONSOR STUDY NO. 211-DIPE-S 

GASOLINE DIPE VAPOR CONDENSATE:

A 13-WEEK WHOLE-BODY INHALATION TOXICITY STUDY IN RATS 

WITH NEUROTOXICITY ASSESSMENTS AND 4-WEEK IN VIVO 

GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS

Final Report

Volume II of IV

Submitted to: American Petroleum Institute (API)

1220 L Street, Northwest

Washington, DC 20005-4070

Attn: Thomas M. Gray, M.S., D.A.B.T.

Date: 31 May 2013

Page 381 of 1446
Huntingdon Life Sciences 00-6130 Page 382 

211-DIPE-S Final Report 

TABLE OF CONTENTS

Volume II

COVER PAGE.................................................................................................................381 

TABLE OF CONTENTS .................................................................................................382 

APPENDICES

E. Individual Body Weight Change from Baseline ........................................................383 

F. Individual Feed Consumption Values ........................................................................399 

G. Individual Motor Activity Values ..............................................................................419 

H. Individual Functional Observational Battery Evaluations .........................................435 

I. Individual Hematology Values ..................................................................................472 

J. Individual Coagulation Values ...................................................................................513 

K. Individual Clinical Chemistry Values ........................................................................530 

L. Individual Organ Weights and Brain Measurements .................................................591 

M. Individual Animal Gross and Microscopic Observations ..........................................630 
26-SEP-2003 10:02 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

MALES 

ANIMAL# 

MEAN 

S.D. 

N 

1051 1052 1053 1054 1055 1056 1057 1058 1059 1060 1061 1062 1063 1064 1065 1066 

1067 

1068 1069 

1070 

GROUP 1 0 MG/M3 

WEEK OF STUDY 

0-1 

42 41 

39 39 32 

43 

33 

48 

21 

38 

45 

39 

35 

45 

33 36 

27 26 22 

39 36 

7.5 

20 

0-2 

76 77 72 74 

54 

77 

63 

83 

48 

77 75 74 73 73 

65 

54 

61 

50 

53 

66 67 

10.6 

20 

APPENDIX E 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

0-3 

101 

95 97 99 

86 

101 

82 

110 

68 

97 

105 107 101 

99 93 

88 

90 

77 79 

97 94 

10.8 

20 

0-4 

117 

123 

127 

122 

90 

117 110 111 

83 

108 125 115 137 127 114 132 110 

94 98 

117 114 

14.0 

20 

INDIVIDUAL BODY WEIGHT CHANGE FROM BASELINE (GRAMS) 

0-5 

133 

132 140 139 111 145 

128 

139 105 

143 

149 140 135 154 136 145 131 114 105 134 

133 

13.8 

20 

0-6 

143 

141 158 149 

124 

158 

142 

161 119 156 168 157 165 162 160 169 145 133 

123 

152 149 

15.0 

20 

0-7 

164 162 175 170 144 168 161 196 139 194 

193 

177 

188 

188 

185 

198 176 153 148 185 173 

18.0 

20 

0-8 

174 174 

192 176 156 

182 

168 

204 

160 

202 206 

193 

213 

205 

203 212 190 160 161 187 186 

18.8 

20 

0-9 

191 185 

212 

193 

168 192 185 

220 

177 

218 225 214 227 221 

214 

230 207 

176 173 

205 202 

19.9 

20 

0-10 

197 198 

233 204 

180 

207 

193 

231 

191 195 233 

234 242 232 218 239 215 

196 

193 

220 212 

19.3 

20 

0-11 

218 206 246 

209 

194 

222 211 247 

206 

218 249 249 

253 253 

234 250 227 211 204 229 227 

19.5 

20 

0-12 

227 211 260 209 

203 

236 221 

254 

210 

223 

254 260 265 257 250 

262 

239 225 

216 248 

237 

20.8 

20 

P
26-SEP-2003 10:02 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

MALES GROUP 2 2000 MG/M3 

WEEK OF STUDY 

ANIMAL# 0-1 0-2 

APPENDIX E 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL BODY WEIGHT CHANGE FROM BASELINE (GRAMS) 

0-3 0-4 0-5 0-6 0-7 0-8 0-9 0-10 0-11 0-12 

-----------------------------------------------------------------------------------------------------------------

2051 37 71 94 115 125 151 164 185 199 205 217 181 2052 26 53 81 96 126 134 154 162 171 175 185 193 2053 37 62 91 115 127 147 172 185 199 213 226 231 2054 30 61 92 118 128 140 160 172 186 193 201 210 

2055 27 63 83 113 133 161 179 187 201 216 229 238 

2056 45 74 105 138 149 168 189 199 213 219 228 237 2057 36 66 96 122 138 152 169 183 200 205 215 224 2058 38 69 109 135 157 178 205 221 242 248 266 269 2059 33 69 94 124 139 158 180 204 222 223 245 258 

2060 43 75 102 128 144 165 184 201 209 223 238 248 

MEAN 35 66 95 120 136 155 175 190 204 212 225 229 

S.D. 6.3 6.6 8.8 12.2 10.8 13.3 15.2 16.9 19.3 19.6 22.6 27.7 

N 10 10 10 10 10 10 10 10 10 10 10 10 

P
26-SEP-2003 10:02 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

MALES GROUP 3 10000 MG/M3 

WEEK OF STUDY 

ANIMAL# 0-1 0-2 

3051 24 52 

3052 30 70 

3053 17 55 

3054 33 64 3055 33 73 3056 25 51 3057 47 81 3058 39 61 

3059 29 60 

3060 29 52 

MEAN 31 62 

S.D. 8.4 10.2 

N 10 10 

APPENDIX E 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL BODY WEIGHT CHANGE FROM BASELINE (GRAMS) 

0-3 0-4 0-5 0-6 0-7 0-8 0-9 0-10 0-11 0-12 

71 97 113 134 162 176 187 207 215 221 

102 133 152 175 198 218 225 238 242 256 

72 107 124 138 174 178 190 205 213 216 

89 119 133 154 183 195 215 222 240 249 

100 117 136 160 181 206 217 229 241 249 

67 90 100 124 144 161 171 184 197 200 

104 130 153 176 201 219 244 269 289 299 

81 107 122 138 158 175 188 200 205 213 81 100 126 147 161 177 188 204 218 223 

73 102 120 138 157 170 181 189 202 209 

84 110 128 148 172 187 201 215 226 233 

13.9 14.3 16.5 17.6 18.7 20.7 23.4 25.6 27.6 29.8 

10 10 10 10 10 10 10 10 10 10 

P
26-SEP-2003 10:02 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

MALES 

ANIMAL# 

MEAN 

S.D. 

N 

4051 4052 

4053 

4054 

4055 4056 4057 

4058 

4059 4060 

4061 

4062 4063 4064 

4065 4066 

4067 4068 

4069 4070 

GROUP 4 20000 MG/M3 

WEEK OF STUDY 

0-1 

25 28 

26 

32 

26 

33 

42 

35 

19 

29 

33 

28 

37 36 

29 27 

40 47 

19 41 

32 

7.5 

20 

0-2 

51 

60 

53 53 57 

64 68 

71 

49 

60 

58 

55 

75 

59 58 

61 

70 

87 

40 

70 

61 

10.4 

20 

APPENDIX E 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

0-3 

75 

85 

77 

80 

77 

88 

100 

97 

69 

93 

85 

78 

111 

83 82 

87 

92 

116 

62 

99 

87 

13.4 

20 

0-4 

104 109 

102 

103 

93 

105 109 

110 

87 

112 109 

69 

139 104 109 

124 

119 149 

81 

118 

108 

18.0 

20 

INDIVIDUAL BODY WEIGHT CHANGE FROM BASELINE (GRAMS) 

0-5 

124 129 

129 122 114 

127 

134 

124 

110 139 117 

77 

155 118 123 

138 

133 

175 

90 

136 

126 

20.6 

20 

0-6 

141 148 

143 143 123 143 

157 

144 129 

152 138 

95 

177 

139 

136 

155 

151 

197 107 158 144 

21.9 

20 

0-7 

169 170 170 169 141 171 180 

177 

154 

177 161 

109 

206 

173 

159 187 

173 

231 

128 182 169 

25.6 

20 

0-8 

184 176 

182 

185 

156 

158 

193 

186 171 194 

178 

118 

221 

186 

173 

198 

181 

248 146 195 181 

26.7 

20 

0-9 

201 

188 

201 

194 

175 182 

210 

191 190 

220 

191 

133 

237 

205 

191 

214 

199 

276 157 

221 

199 

29.1 

20 

0-10 

216 

195 

220 209 

185 194 

216 205 201 239 207 

147 

247 

214 196 

221 

207 295 

166 

236 

211 

30.6 

20 

0-11 

235 

207 

236 

217 

186 203 

226 

220 200 250 

220 

152 

263 

232 

217 

239 

217 

316 

174 

255 

223 

34.7 

20 

0-12 

236 219 

243 

219 

191 

210 231 

223 

209 

263 233 

161 

267 232 225 246 229 

333 

185 

263 231 

35.7 

20 

P
26-SEP-2003 lO:OS 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

APPENDIX E 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

MALES GROUP 1 

ANIMAL# 

lOSl 

10S2 

10S3 

10S4 

lOSS 

10S6 10S7 10S8 

10S9 

1060 

1061 1062 

1063 

1064 

106S 

1066 

1067 1068 

1069 

1070 

MEAN 

S.D. 

N 

0 MG/M3 

WEEK OF STUDY 

0-13 0-14 0-lS 

243 2S6 264 

219 220 232 

267 266 273 202 21S 231" 

209 226 236 

247 2SS 27S 

218 226 232 

266 28S 281 

227 223 239 

224 224 233 

TS TS TS TS 

TS TS 

TS 

TS TS 

TS 

232 240 2SO 

22.7 23.7 20.9 

10 10 10 

TS=Terminal Sacrifice 

INDIVIDUAL BODY WEIGHT CHANGE FROM BASELINE (GRAMS) 

0-16 0-17 

272 274 

233 240 

279 281 

239 241 248 2S9 

288 297 

237 239 

30S 309 

2Sl 249 

239 244 

2S9 263 

2S.O 2S.S 

10 10 

P
26-SEP-2003 10:19 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

MALES GROUP 4 20000 MG/M3 

WEEK OF STUDY 

ANIMAL# 0-13 0-14 

4051 236 253 4052 219 234 4053 246 261 4054 225 240 4055 184 203 4056 214 221 4057 231 246 4058 232 238 4059 214 222 4060 269 270 4061 TS 4062 TS 4063 TS 4064 TS 4065 TS 4066 TS 4067 TS 4068 TS 4069 TS 4070 TS 

MEAN 227 239 

S.D. 22.4 20.1 

N 10 10 

TS=Terminal Sacrifice 

APPENDIX E 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL BODY WEIGHT CHANGE FROM BASELINE (GRAMS) 

0-15 0-16 0-17 

267 269 276 244 255 259 271 281 296 

266 279 282 

220 221 229 

237 244 247 

256 270 269 

250 267 274 

237 251 263 

296 305 305 

254 264 270 

21.9 23.0 22.3 

10 10 10 

P
26-SEP-2003 10:20 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

FEMALES GROUP 1 

ANIMAL# 

MEAN 

S.D. 

N 

1551 

1552 1553 1554 1555 1556 1557 1558 1559 1560 1561 1562 1563 1564 1565 1566 

1567 1568 1569 

1570 

0 MG/M3 

WEEK OF STUDY 

0-1 

9 

14 

2 

18 

9 

19 12 11 

13 

15 

16 18 

27 

7 

22 24 

14 

19 

13 

20 

15 

6.1 

20 

0-2 

15 

25 

16 

28 26 27 

21 

29 24 

36 

25 27 

42 

32 

28 

42 

27 

56 

20 

34 

29 

9.5 

20 

APPENDIX E 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

0-3 

21 

35 

28 

37 33 

40 

25 

40 

21 

37 31 

40 48 

38 

49 

37 

42 

67 

32 

48 

37 

10.6 

20 

0-4 

31 

47 

37 

58 

so so 

34 

44 

38 

53 

44 49 

53 

43 

50 

so 

55 

71 

45 

56 

48 

9.1 

20 

INDIVIDUAL BODY WEIGHT CHANGE FROM BASELINE (GRAMS) 

0-5 

31 

58 

40 

67 

55 59 

38 

49 43 

56 

54 

55 

63 

45 

69 

56 

65 

90 

so 

59 55 

13.1 

20 

0-6 

38 

56 

36 

70 

64 61 

42 

51 

44 

56 

60 

54 

63 

51 51 

63 

70 

91 

44 

69 

57 

13.1 

20 

0-7 

40 

66 

47 

83 

74 72 

55 58 

47 

58 

60 63 65 

70 75 

68 

78 

112 

63 

88 

67 

16.1 

20 

0-8 

45 

74 

49 

94 

77 

68 

54 

63 

55 

72 70 70 

67 62 

73 

68 

97 

117 

68 

95 

72 

17.6 

20 

0-9 

54 

79 

53 

98 78 72 

64 69 60 

79 

81 

68 

82 

61 

81 

75 

107 117 

71 

92 

77 

16.6 

20 

0-10 

59 

88 

63 

98 

92 

84 

72 75 

61 

87 

84 

74 

84 

72 74 

82 

103 132 

72 

101 

83 

17.1 

20 

0-11 

64 

92 

66 

104 

96 

88 

67 

78 

60 

102 

78 

80 85 

78 

93 

71 

104 128 

81 

95 

86 

16.8 

20 

0-12 

61 

89 

67 

112 

97 

89 

74 

82 

70 

88 

104 

79 

82 

77 

89 

76 

115 127 

79 

97 

88 

17.1 

20 

P
26-SEP-2003 10:20 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

FEMALES GROUP 2 

ANIMAL# 

2000 MG/M3 

WEEK OF STUDY 

APPENDIX E 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL BODY WEIGHT CHANGE FROM BASELINE (GRAMS) 

0-1 0-2 0-3 0-4 0-5 0-6 0-7 0-8 0-9 0-10 0-11 0-12 

------------------------------------------------------------------------------------------------------------------

2551 17 31 41 49 57 57 71 71 76 88 91 85 2552 18 20 38 50 53 52 66 71 71 72 75 79 

2553 13 12 27 42 40 33 49 56 57 57 63 71 2554 27 37 40 50 60 67 71 79 88 95 98 100 2555 23 34 50 60 64 77 77 79 86 91 96 93 2556 20 33 45 52 58 63 70 83 92 101 94 105 

2557 23 49 62 64 63 71 87 86 90 105 98 110 2558 4 10 20 36 35 35 49 54 53 55 61 68 2559 21 36 44 45 58 66 65 68 72 74 75 82 2560 24 40 48 62 65 74 86 94 92 109 104 118 

MEAN 19 30 41 51 55 60 69 74 78 85 85 91 

S.D. 6.5 12.5 11.8 8.9 10.0 15.2 12.9 12.7 14.2 19.2 15.5 16.7 

N 10 10 10 10 10 10 10 10 10 10 10 10 

P
26-SEP-2003 10:20 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

FEMALES GROUP 3 10000 MG/M3 

WEEK OF STUDY 

ANIMAL# 0-1 0-2 

APPENDIX E 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL BODY WEIGHT CHANGE FROM BASELINE (GRAMS) 

0-3 0-4 0-5 0-6 0-7 0-8 0-9 0-10 0-11 0-12 

------------------------------------------------------------------------------------------------------------------

3551 23 41 44 64 70 73 84 88 97 105 103 106 3552 27 47 55 59 72 75 88 92 106 112 116 113 3553 0 21 22 35 38 43 53 49 60 70 60 58 3554 10 25 35 58 57 62 72 85 99 105 116 121 3555 8 23 37 52 55 55 70 70 83 81 90 96 3556 29 52 66 71 85 92 98 104 121 129 131 127 3557 17 25 27 42 50 52 60 70 79 82 80 89 3558 18 36 52 59 68 77 85 76 92 100 104 101 3559 21 29 29 51 66 77 74 93 109 102 94 111 

3560 25 44 47 68 79 80 97 99 107 102 112 108 

MEAN 18 34 41 56 64 69 78 83 95 99 101 103 

S.D. 9.2 11.0 13.9 11.3 14.2 15.0 14.8 16.5 17.5 17.0 20.4 19.3 

N 10 10 10 10 10 10 10 10 10 10 10 10 

P
26-SEP-2003 10:20 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

FEMALES GROUP 4 

ANIMAL# 

4551 4552 4553 4554 4555 4556 4557 4558 4559 4560 

4561 

4562 4563 4564 4565 4566 

4567 4568 4569 4570 

MEAN 

S.D. 

N 

20000 MG/M3 

WEEK OF STUDY 

0-1 

13 

-2 

7 

3 

6 

17 

24 

16 12 

15 

13 

8 

12 

25 

21 

17 

16 

11 14 10 

13 

6.5 

20 

0-2 

24 

10 

33 

16 

21 26 

37 32 

25 29 23 

15 

24 

39 32 

29 

30 

28 25 27 

26 

7.1 

20 

APPENDIX E 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

0-3 

31 

19 

36 

18 

29 

32 

56 

42 

29 

37 32 

27 

36 

52 

30 36 32 38 

25 

37 34 

9.2 

20 

0-4 

32 

27 

45 

29 

39 

46 41 47 

38 

53 

47 

35 39 

58 

35 

52 

45 40 

38 37 

41 

7.9 

20 

INDIVIDUAL BODY WEIGHT CHANGE FROM BASELINE (GRAMS) 

0-5 

40 

37 

49 

33 

45 

55 

57 

60 

46 

62 

54 

38 

55 

70 

39 

51 

53 53 

48 

52 

50 

9.2 

20 

0-6 

45 

33 

57 

37 

48 

58 59 

69 

52 

68 

59 

43 

53 

71 

47 

59 

58 

62 

53 56 54 

10.1 

20 

0-7 

51 

41 

67 

42 

58 

62 

56 

74 

55 

65 

61 

53 

41 

85 

57 

65 66 66 

47 

70 

59 

11.5 

20 

0-8 

54 

44 

69 

51 

61 

70 76 70 

56 

80 

70 

54 

58 

84 

52 

67 

72 76 

62 

59 

64 

10.7 

20 

0-9 

62 

45 

70 

61 67 

80 

78 

81 

63 

85 

78 

54 

62 

88 

59 

73 

88 84 

68 

75 71 

11.8 

20 

0-10 

66 

51 

76 

62 

70 

86 

72 

91 

71 

91 

84 

56 

74 

83 

60 

76 

87 

80 

71 

82 

74 

11.4 

20 

0-11 

72 

52 

85 

69 

72 

83 

70 

91 

71 

85 87 

61 

69 

96 

67 

81 

86 83 

68 

83 

77 

11.0 

20 

0-12 

65 

59 

81 

77 72 

93 

81 

91 69 

95 

91 64 69 

92 

66 

84 89 

91 

80 

78 79 

11.3 

20 

P
26-SEP-2003 10:20 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

FEMALES GROUP 1 0 MG/M3 

WEEK OF STUDY 

ANIMAL# 0-13 0-14 

1551 65 71 1552 91 94 1553 63 67 1554 114 109 1555 93 96 

1556 82 87 1557 78 63 1558 77 85 1559 64 66 1560 79 84 1561 TS 1562 TS 1563 TS 1564 TS 1565 TS 1566 TS 

1567 TS 

1568 TS 1569 TS 1570 TS 

MEAN 81 82 

S.D. 15.8 15.2 

N 10 10 

TS=Terminal Sacrifice 

APPENDIX E 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL BODY WEIGHT CHANGE FROM BASELINE (GRAMS) 

0-15 0-16 0-17 

75 77 71 

98 97 95 77 69 78 

112 121 127 105 101 100 

92 91 92 

64 67 72 

84 84 81 

69 72 80 

83 85 74 

86 87 87 

15.7 16.8 17.1 

10 10 10 

P
26-SEP-2003 10:50 

Huntingdon Life Sciences 00-6130N 

Neurotoxicity Sub-Group 

MALES GROUP 4 

ANIMAL# 

4071 4072 4073 4074 4075 

MEAN 

S.D. 

N 

20000 MG/M3 

WEEK OF STUDY 

APPENDIX E 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL BODY WEIGHT CHANGE FROM BASELINE (GRAMS) 

0-1 0-2 0-3 0-4 0-5 0-6 0-7 0-8 0-9 0-10 0-11 0-12 

31 56 82 103 127 138 165 161 182 192 202 218 

48 87 119 141 173 201 218 244 259 281 296 312 

37 71 100 121 140 159 178 189 210 228 240 253 

42 76 97 118 139 131 136 130 137 139 147 133 

38 73 97 121 142 156 177 198 210 223 238 247 39 73 99 121 144 157 175 184 200 212 225 233 

6.3 11.0 13.0 13.4 17.4 27.1 29.6 42.5 44.6 52.4 55.2 65.4 

5 5 5 5 5 5 5 5 5 5 5 5 

0-13 

215 

308 

254 

129 

250 231 

66.2 

5 

P
26-SEP-2003 13:49 

Huntingdon Life Sciences 00-6130n 

Neurotoxicity Sub-Group 

FEMALES GROUP 1 

ANIMAL# 

0 MG/M3 

WEEK OF STUDY 

APPENDIX E 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL BODY WEIGHT CHANGE FROM BASELINE (GRAMS) 

0-1 0-2 0-3 0-4 0-5 0-6 0-7 0-8 0-9 0-10 0-ll 0-12 0-13 

------------------------------------------------------------------------------------------------------------------

1571 16 30 35 47 63 63 68 69 77 80 79 90 83 1572 23 42 51 59 75 76 84 102 99 109 ll4 ll8 103 1573 23 49 55 72 82 89 102 106 ll3 123 123 140 140 1574 24 46 55 60 83 85 94 96 105 110 ll5 ll9 121 

1575 21 21 34 42 46 43 58 55 58 56 64 65 61 

MEAN 21 37 46 56 69 71 81 86 90 96 99 106 102 

S.D. 3.1 ll.6 10.9 ll.8 15.5 18.6 18.5 22.3 22.6 27.2 26.0 29.0 31.0 

N 5 5 5 5 5 5 5 5 5 5 5 5 5 

P
26-SEP-2003 13:49 

Huntingdon Life Sciences 00-6130n 

Neurotoxicity Sub-Group 

FEMALES GROUP 2 

ANIMAL# 

2000 MG/M3 

WEEK OF STUDY 

APPENDIX E 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL BODY WEIGHT CHANGE FROM BASELINE (GRAMS) 

0-1 0-2 0-3 0-4 0-5 0-6 0-7 0-8 0-9 0-10 0-ll 0-12 0-13 

------------------------------------------------------------------------------------------------------------------

2561 20 32 42 40 53 54 60 51 64 70 73 71 70 2562 6 19 56 53 so 66 78 78 81 95 103 ll3 92 

2563 28 41 48 62 74 69 73 82 86 90 95 103 llO 2564 20 36 45 45 61 61 71 73 84 87 88 104 96 2565 20 32 49 56 69 63 80 81 85 83 98 105 98 

MEAN 19 32 48 51 61 62 73 73 80 85 92 99 93 

S.D. 8.1 8.2 5.3 8.9 10.4 5.5 7.8 12.9 9.3 9.5 ll.6 16.0 14.4 

N 5 5 5 5 5 5 5 5 5 5 5 5 5 

P
26-SEP-2003 13:49 

Huntingdon Life Sciences 00-6130n 

Neurotoxicity Sub-Group 

FEMALES GROUP 3 

ANIMAL# 

10000 MG/M3 

WEEK OF STUDY 

APPENDIX E 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL BODY WEIGHT CHANGE FROM BASELINE (GRAMS) 

0-1 0-2 0-3 0-4 0-5 0-6 0-7 0-8 0-9 0-10 0-11 0-12 0-13 

-----------------------------------------------------------------------------------------------------------------

3561 2 21 33 39 46 52 63 72 70 81 97 94 91 3562 10 17 15 31 36 35 32 44 48 49 48 64 55 3563 16 30 30 44 49 54 62 65 64 68 67 75 73 3564 15 28 41 50 59 70 82 76 87 95 101 102 100 3565 23 40 50 71 83 88 94 109 117 118 118 136 140 

MEAN 13 27 34 47 55 60 66 73 77 82 86 94 92 

S.D. 7.9 8.9 13.1 15.1 18.1 20.2 23.6 23.5 26.3 26.5 28.1 27.6 32.0 

N 5 5 5 5 5 5 5 5 5 5 5 5 5 

P
26-SEP-2003 13:49 

Huntingdon Life Sciences 00-6130n 

Neurotoxicity Sub-Group 

FEMALES GROUP 4 20000 MG/M3 

WEEK OF STUDY 

ANIMAL# 0-1 0-2 

APPENDIX E 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL BODY WEIGHT CHANGE FROM BASELINE (GRAMS) 

0-3 0-4 0-5 0-6 0-7 0-8 0-9 0-10 0-11 0-12 0-13 

-------------------------------------------------------------------------------------------------------------------

4571 26 41 47 57 75 83 96 94 111 120 127 127 125 4572 28 42 56 55 64 67 74 73 77 82 93 102 99 4573 3 24 37 47 54 65 68 70 68 82 84 89 66 4574 14 13 27 34 40 34 46 51 52 49 62 66 85 4575 23 42 49 51 70 76 82 83 97 104 99 100 105 

MEAN 19 32 43 49 61 65 73 74 81 87 93 97 96 

S.D. 10.4 13.0 11.4 9.1 14.1 19.0 18.6 15.9 23.5 26.8 23.6 22.3 22.0 

N 5 5 5 5 5 5 5 5 5 5 5 5 5 

P
26-SEP-2003 13:52 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

MALES 

ANIMAL# 

MEAN 

S.D. 

N 

1051 1052 

1053 1054 1055 1056 1057 1058 1059 1060 

1061 

1062 

1063 

1064 1065 

1066 

1067 1068 1069 

1070 

GROUP 1 0 MG/M3 

WEEK OF STUDY 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

0 1 2 3 4 5 6 7 8 9 10 11 

100 

97 

86 

94 

101 100 

96 99 96 

103 

95 

102 

96 

100 104 105 

95 

91 

89 

99 

97 

4.9 

20 

79 77 

73 78 

85 83 

79 78 77 

87 

78 

81 

83 84 

90 

84 

78 74 72 

81 80 

4.6 

20 

70 70 

66 68 

75 

71 71 70 70 

80 

69 

73 71 73 75 

68 

74 

69 65 

72 

71 

3.4 

20 

66 

61 

62 

63 

71 

64 64 65 63 

72 

63 

72 

66 

72 

68 

67 

70 70 

61 65 

66 

3.7 

20 

64 

58 

59 

61 

63 

58 

62 

54 58 

61 

60 63 66 67 66 67 

67 64 

58 

63 62 

3.7 

20 

63 

55 

54 57 

64 

58 56 57 

63 69 60 62 

53 

63 66 

59 

61 

59 

55 59 

60 

4.2 

20 

60 

57 

56 

56 

63 

59 

59 

59 

58 

66 

59 

60 

57 59 

66 

59 

62 63 

58 

61 60 

2.9 

20 

59 55 

55 

52 59 

53 

55 59 

56 

62 

54 

57 

56 

59 

58 

57 

60 

56 54 

59 57 

2.5 

20 

52 

51 

52 

50 

56 53 

51 

54 53 58 

so 

55 55 55 

56 

51 

53 

51 

48 

55 53 

2.5 

20 

52 

so 

so 

47 

55 53 52 53 54 55 

49 

54 

51 

54 

51 

46 

54 53 

so 

54 52 

2.5 

20 

52 

so 

50 

49 

54 52 

51 

53 

51 

42 48 

56 

so 

55 

49 45 

51 

52 50 53 

51 

3.2 

20 

51 

so 

46 45 

55 53 

49 

so so 

56 

46 

52 

46 

54 

48 42 

51 51 

47 

so so 

3.6 

20 

12 

47 48 

46 44 

53 53 

49 

48 

48 

50 

45 

49 

47 

so 

47 43 

48 

51 

47 

50 

48 

2.7 

20 

P
26-SEP-2003 13:52 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

MALES GROUP 2 2000 MG/M3 

WEEK OF STUDY 

ANIMAL# 0 1 

2051 89 74 2052 93 76 2053 94 78 2054 102 83 2055 91 71 2056 101 80 2057 92 77 2058 100 80 

2059 96 78 

2060 98 78 

MEAN 96 77 

S.D. 4.7 3.2 

N 10 10 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

2 3 4 5 6 7 8 9 10 11 

69 66 58 58 63 58 56 54 52 50 

72 68 63 61 62 59 57 54 53 51 70 65 61 58 58 54 50 48 47 45 

72 67 64 58 56 55 52 52 51 48 

69 65 61 56 55 51 48 49 48 45 

75 71 70 60 59 56 51 48 46 45 

65 62 60 56 54 49 47 47 44 44 

70 67 66 59 59 58 56 53 50 49 74 71 67 60 62 60 57 54 52 52 72 64 63 59 57 52 50 49 48 47 71 67 63 59 59 55 52 51 49 48 

2.7 3.0 3.6 1.8 3.0 3.5 3.8 2.8 3.0 2.8 

10 10 10 10 10 10 10 10 10 10 

12 

39 

50 

43 48 43 45 

43 

49 

52 

45 46 

3.9 

10 

P
26-SEP-2003 13:52 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

MALES GROUP 3 

ANIMAL# 

10000 MG/M3 

WEEK OF STUDY 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

0 1 2 3 4 5 6 7 8 9 10 11 12 

------------------------------------------------------------------------------------------------------------------

3051 95 88 71 69 68 59 65 60 54 54 53 49 48 3052 91 63 67 62 60 54 53 50 49 46 46 44 45 3053 93 70 72 67 68 58 63 61 54 52 52 49 47 

3054 97 80 70 66 61 56 58 55 53 52 50 50 48 

3055 97 78 80 69 64 60 66 60 58 56 52 48 47 3056 98 79 75 70 SF 64 73 63 61 57 53 51 48 

3057 105 80 67 69 67 64 59 57 53 55 56 49 46 3058 96 83 67 64 65 55 57 55 53 so 51 45 46 

3059 98 79 73 66 60 60 60 54 53 48 48 47 44 

3060 95 77 72 67 64 60 62 58 55 52 52 51 so 

MEAN 96 78 71 67 64 59 61 57 54 52 51 48 47 

S.D. 3.7 6.9 4.1 2.5 3.1 3.3 5.7 3.9 3.2 3.4 2.8 2.3 1.6 

N 10 10 10 10 9 10 10 10 10 10 10 10 10 

SF=Spilled Feeder 

P
26-SEP-2003 13:52 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

MALES 

ANIMAL# 

4051 4052 

4053 4054 4055 4056 4057 4058 4059 4060 4061 4062 4063 4064 4065 4066 

4067 4068 4069 4070 

MEAN 

S.D. 

N 

GROUP 4 

EF=Empty Feeder 

20000 MG/M3 

WEEK OF STUDY 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

0 1 2 3 4 5 6 7 8 9 10 11 

93 

97 

100 

98 93 93 

107 

103 

102 102 

92 95 93 

88 

91 95 

102 

109 

94 

91 

97 

5.7 

20 

71 75 

78 79 72 71 

88 

78 78 

84 

76 75 74 71 76 75 

79 

80 

71 77 76 

4.4 

20 

65 69 

71 72 71 

66 

77 78 73 70 70 70 

69 62 

68 68 

73 

69 65 68 

70 

3.8 

20 

61 63 

64 

69 

65 62 

73 75 70 72 

67 

65 

66 

59 

65 66 

68 64 63 65 66 

4.0 

20 

59 

60 

64 63 60 

56 

63 68 66 61 61 

53 

61 

58 

63 64 

66 60 61 61 61 

3.5 

20 

55 

55 

61 60 

58 55 

68 63 65 63 

54 

49 

55 

52 

57 

60 

59 

58 54 56 58 

4.7 

20 

53 55 

56 58 58 54 

69 66 63 60 60 63 

55 57 

58 56 

58 

57 55 

56 58 

4.2 

20 

53 

52 

55 

60 

55 

54 

EF 

60 

59 

58 

55 57 56 

54 57 56 

54 55 

63 

50 56 

3.0 

19 

52 50 

53 56 54 

47 

67 

57 58 54 54 54 

51 

52 54 

51 

49 49 

51 

50 53 

4.2 

20 

52 

49 

53 53 54 

48 

67 

54 

57 

55 51 54 

50 51 51 51 

49 49 

52 52 53 

4.1 

20 

50 

47 

51 

52 52 50 

60 

53 53 

52 

50 

55 

49 49 

51 50 

49 48 

50 

53 

51 

2.8 

20 

41 45 

30 

44 49 49 

59 

52 

50 

49 48 

52 

49 46 49 48 

48 

46 48 49 48 

5.5 

20 

12 44 44 

46 48 

49 49 

56 

49 

50 

47 47 

52 

46 45 48 47 

46 45 47 47 48 

2.7 

20 

P
26-SEP-2003 14:00 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

MALES GROUP 1 0 MG/M3 

WEEK OF STUDY 

ANIMAL# 13 14 

1051 47 49 1052 48 51 1053 43 44 1054 40 44 

lOSS so 53 

1056 so 54 1057 45 45 1058 46 49 1059 46 44 1060 49 51 1061 TS TS 1062 TS TS 1063 TS TS 1064 TS TS 1065 TS TS 1066 TS TS 1067 TS TS 1068 TS TS 1069 TS TS 1070 TS TS 

MEAN 46 48 

S.D. 3.0 3.9 

N 10 10 

TS=Terminal Sacrifice 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

15 

48 

48 43 45 

51 

54 

46 47 47 

51 

48 

3.2 

10 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

16 

47 46 

42 

46 

51 

52 

46 49 46 48 47 

2.9 

10 

17 46 

49 40 

41 

51 

so 

45 47 43 48 

46 

3.5 

10 

P
26-SEP-2003. 14:00 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

MALES GROUP 4 20000 MG/M3 

WEEK OF STUDY 

ANIMAL# 13 14 

4051 42 47 4052 42 45 

4053 44 48 4054 46 51 4055 42 53 4056 45 50 4057 48 57 4058 46 50 4059 47 53 4060 45 47 

4061 TS TS 

4062 TS TS 4063 TS TS 4064 TS TS 4065 TS TS 4066 TS TS 

4067 TS TS 4068 TS TS 4069 TS TS 4070 TS TS 

MEAN 45 so 

S.D. 2.2 3.7 

N 10 10 

TS=Terminal Sacrifice 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

15 

46 

45 

47 

54 

53 

52 59 55 

52 

49 

51 

4.3 

10 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

16 

46 44 

47 

so 

47 

so 

58 

54 51 

47 

49 

4.2 

10 

17 

43 42 

47 49 49 47 

55 

51 

49 

46 

48 

3.7 

10 

P
26-SEP-2003 14:04 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

FEMALES GROUP 1 

ANIMAL# 

MEAN 

S.D. 

N 

1551 

1552 

1553 1554 1555 1556 1557 1558 1559 1560 

1561 

1562 1563 1564 

1565 

1566 1567 

1568 1569 

1570 

0 MG/M3 

WEEK OF STUDY 

0 

101 

95 94 99 99 95 94 94 

97 93 90 

100 

106 

90 

108 

88 

100 

104 

CF 

96 97 

5.3 

19 

1 

80 80 82 

79 

82 84 

76 

81 

76 77 77 78 

84 

79 

92 

83 

81 

82 

CF 

83 

81 

3.6 

19 

CF=Contaminated Feeder 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

2 

71 76 

81 

76 79 72 75 76 71 

76 

75 74 

73 79 

85 

78 

80 

77 

CF 

79 77 

3.7 

19 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

3 

68 

73 

79 72 73 

80 

73 73 

68 68 

71 72 

68 

73 76 

72 

81 

70 70 79 73 

4.1 

20 

4 

67 

75 75 71 72 70 71 

68 

70 

69 69 67 69 69 

79 74 77 

64 65 

73 71 

3.9 

20 

5 

60 

71 72 

67 69 66 

68 

72 

65 64 

71 

64 62 66 

72 

69 66 60 

CF 

67 67 

3.8 

19 

6 

61 65 

71 

65 69 

73 

69 

67 61 

59 

71 

62 60 66 64 65 

75 

63 

CF 

68 66 

4.5 

19 

7 

61 

70 71 

68 65 69 68 63 60 60 

64 60 

59 

64 

73 

61 

75 

62 63 

70 

65 

4.8 

20 

8 

62 67 

72 

66 

65 65 63 65 63 61 66 61 

59 

59 

62 61 

70 

58 57 

64 63 

3.8 

20 

9 

63 61 

74 

60 65 68 

64 67 60 

57 

65 60 60 60 68 62 68 

56 

60 

58 

63 

4.6 

20 

10 

66 63 69 

61 

64 

70 

63 64 

58 

59 

61 

60 

57 

60 60 

58 

60 

58 

62 64 62 

3.6 

20 

11 

58 58 

67 63 

56 58 

58 59 

55 

64 

51 59 

54 56 

65 

50 

60 

52 

59 

58 58 

4.5 

20 

12 

54 

57 

64 

58 58 59 

62 60 

59 

48 

63 

56 55 56 55 

60 

57 51 56 57 57 

3.8 

20 

P
26-SEP-2003 14:04 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

FEMALES GROUP 2 

ANIMAL# 

2000 MG/M3 

WEEK OF STUDY 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

0 1 2 3 4 5 6 7 8 9 10 11 12 

------------------------------------------------------------------------------------------------------------------

2551 93 83 74 71 70 62 57 64 61 60 63 59 58 2552 94 80 79 78 70 66 64 65 63 62 62 58 57 

2553 99 85 85 84 SF 70 72 73 70 68 70 68 65 2554 93 84 75 69 70 65 63 61 63 62 61 56 58 2555 90 81 70 70 66 59 62 56 57 57 57 55 56 2556 94 78 74 75 68 63 67 64 70 66 68 62 64 2557 111 95 86 77 69 64 68 64 61 65 67 58 66 2558 113 78 88 81 75 65 72 70 64 64 67 64 62 2559 94 78 74 68 63 62 63 54 56 51 53 54 58 

2560 99 86 68 72 72 65 68 64 63 65 67 59 64 

MEAN 98 83 77 75 69 64 66 64 63 62 63 59 61 

S.D. 8.0 5.1 6.9 5.3 3.5 2.9 4.5 5.8 4.6 5.0 5.4 4.5 3.8 

N 10 10 10 10 9 10 10 10 10 10 10 10 10 

SF=Spilled Feeder 

P
26-SEP-2003 14:04 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

FEMALES GROUP 3 10000 MG/M3 

WEEK OF STUDY 

ANIMAL# 0 1 2 3 4 5 6 7 8 9 10 11 

3551 90 75 74 72 72 65 67 65 60 57 57 55 3552 96 86 81 71 76 72 67 68 71 69 68 62 3553 95 72 77 68 71 67 67 68 67 66 61 55 3554 101 75 70 70 72 59 61 64 63 66 63 59 3555 CF 69 76 76 64 62 67 66 60 59 61 58 3556 97 83 77 73 72 67 69 64 65 66 63 58 

3557 94 CF 68 67 67 59 62 60 63 59 59 61 3558 94 82 76 72 71 62 65 62 61 65 59 56 3559 104 77 66 66 66 60 64 60 61 59 52 55 3560 100 83 80 77 76 70 72 69 65 66 66 62 

MEAN 97 78 75 71 71 64 66 65 64 63 61 58 

S.D. 4.4 5.8 5.0 3.5 3.9 4.5 3.1 3.3 3.5 4.1 4.5 2.8 

N 9 9 10 10 10 10 10 10 10 10 10 10 

CF=Contaminated Feeder 

12 

53 

64 

60 

60 

58 

62 

58 59 

55 

60 

59 

3.1 

10 

P
26-SEP-2003 14:04 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

FEMALES GROUP 4 

ANIMAL# 

MEAN 

S.D. 

N 

4551 4552 

4553 4554 4555 4556 4557 4558 

4559 

4560 4561 4562 

4563 4564 4565 4566 

4567 4568 4569 

4570 

20000 MG/M3 

WEEK OF STUDY 

0 1 2 

89 

99 

93 92 

101 

91 

98 

90 97 

111 

94 98 99 

106 

92 

64 

103 103 101 

95 

96 

9.4 

20 

74 72 

69 68 

75 78 79 78 74 

90 

68 

SF 

90 76 

80 88 

77 

91 

78 78 78 

7.1 

19 

72 76 

75 

70 75 

73 77 75 

69 

84 

71 

88 

79 

82 

77 75 

75 78 

73 74 

76 

4.7 

20 

SF=Spilled Feeder 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

3 

71 72 

65 66 

76 

73 75 72 

68 

80 

71 79 73 79 71 7.5 

73 75 

69 69 

72 

4.2 

20 

4 

66 

70 

64 63 

71 

72 

60 

71 

69 

82 

69 

SF 

70 75 

69 

73 

69 

71 

67 

70 

70 

4.7 

19 

5 

64 68 

67 65 69 66 68 

70 

65 

76 

62 66 

73 77 

68 68 66 

68 

58 

69 68 

4.3 

20 

6 

66 66 

64 65 69 67 65 67 

63 

76 

61 

72 

64 

74 

69 67 

69 65 61 66 67 

3.8 

20 

7 

67 69 

62 

60 66 67 

63 63 60 

73 

64 

70 

55 

67 67 67 

67 65 

59 

65 65 

4.2 

20 

8 

64 

68 

62 

59 

63 65 67 63 62 

77 

63 65 

SF 

66 65 62 

64 

63 61 60 

64 

3.8 

19 

9 

63 61 

57 57 

63 65 

59 

62 60 69 

58 

64 62 66 

62 62 

63 60 

58 

65 62 

3.1 

20 

10 

61 

78 

57 58 59 

63 

59 

60 

59 

70 

56 

66 67 69 61 62 

63 

59 

56 

62 62 

5.4 

20 

11 

58 

64 

53 57 59 58 59 56 56 

64 

55 

62 

55 

68 62 60 

58 59 57 58 59 

3.7 

20 

12 

55 

65 

53 

55 57 

62 60 

57 59 

66 

55 

60 62 69 63 

56 

60 

59 

56 

60 60 

4.1 

20 

P
26-SEP-2003 14:06 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

FEMALES GROUP 1 0 MG/M3 

WEEK OF STUDY 

ANIMAL# 13 14 

1551 54 81 1552 57 59 1553 60 66 1554 54 55 1555 59 61 1556 57 64 1557 55 59 1558 61 67 1559 52 58 1560 49 55 1561 TS TS 

1562 TS TS 

1563 TS TS 

1564 TS TS 1565 TS TS 1566 TS TS 1567 TS TS 1568 TS TS 1569 TS TS 1570 TS TS 

MEAN 56 62 

S.D. 3.8 7.5 

N 10 10 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

15 

61 

57 

64 

55 

55 58 

56 57 

59 

55 

58 

3.0 

10 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

16 

SF 

55 

61 

55 54 59 57 

60 

57 53 

57 

2.7 

9 

17 

56 59 

65 

55 56 

62 63 60 

59 52 

59 

4.1 

10 

SF=Spilled Feeder TS=Terminal Sacrifice 

P
26-SEP-2003 14:06 

Huntingdon Life Sciences 00-6130 

Toxicity Sub-Group (Main and Recovery) 

FEMALES GROUP 4 

ANIMAL# 

4551 4552 

4553 4554 4555 4556 4557 4558 4559 4560 4561 4562 4563 4564 4565 4566 

4567 

4568 

4569 4570 

MEAN 

S.D. 

N 

20000 MG/M3 

WEEK OF STUDY 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

13 14 15 16 17 

55 62 

60 100 

54 56 53 57 58 64 

55 59 55 60 

56 56 

57 60 

62 64 

TS TS 

TS TS TS TS TS TS TS TS TS TS 

TS TS TS TS TS TS TS TS 

56 64 

2.8 13.0 

10 10 

60 

70 

55 56 

66 62 

SF 

56 

58 

71 

62 

6.1 

9 

59 

85 

55 

53 

66 62 

70 

58 59 

73 

64 

9.8 

10 

59 

62 

57 

55 

60 

59 55 

58 

66 

70 

60 

4.7 

10 

SF=Spilled Feeder TS=Terminal Sacrifice 

P
26-SEP-2003 14:10 

Huntingdon Life Sciences 00-6130N 

Neurotoxicity Sub-Group 

MALES GROUP 1 0 MG/M3 

WEEK OF STUDY 

ANIMAL# 0 1 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

2 3 4 5 6 7 8 9 10 11 12 13 

------------------------------------------------------------------------------------------------------------------

1071 104 86 75 71 66 64 58 61 53 52 so so 49 46 

1072 96 82 74 71 66 66 62 60 54 56 52 so 51 43 

1073 100 81 74 66 57 63 64 59 55 52 52 49 51 48 1074 96 81 73 70 64 65 62 60 55 56 51 52 so 48 1075 94 77 67 63 61 58 55 53 49 so so 47 46 45 

MEAN 98 82 73 68 63 63 60 59 53 53 51 49 49 46 

S.D. 3.8 3.2 3.4 3.3 3.5 3.2 3.5 3.3 2.4 2.7 1.1 1.7 2.0 2.2 

N 5 5 5 5 5 5 5 5 5 5 5 5 5 5 

P
26-SEP-2003 14:10 

Huntingdon Life Sciences 00-6130N 

Neurotoxicity Sub-Group 

MALES GROUP 2 

ANIMAL# 

2000 MG/M3 

WEEK OF STUDY 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

0 1 2 3 4 5 6 7 8 9 10 11 12 13 

------------------------------------------------------------------------------------------------------------------

2061 96 77 72 65 64 64 58 53 53 53 51 48 48 44 2062 96 81 73 65 61 61 55 53 51 so so 47 38 42 2063 92 80 SF 67 62 63 58 56 51 49 49 47 44 44 2064 93 78 75 70 64 61 59 57 53 55 53 51 so 46 2065 105 88 75 69 61 60 60 55 so 53 48 51 48 46 

MEAN 97 81 74 67 62 62 58 55 52 52 so 49 46 44 

S.D. 5.2 4.4 1.7 2.2 1.3 1.5 1.6 1.7 1.3 2.2 1.8 2.1 4.8 1.6 

N 5 5 4 5 5 5 5 5 5 5 5 5 5 5 

SF=SpHled Feeder 

P
26-SEP-2003 14:10 

Huntingdon Life Sciences 00-6130N 

Neurotoxicity Sub-Group 

MALES GROUP 3 

ANIMAL# 

10000 MG/M3 

WEEK OF STUDY 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

0 1 2 3 4 5 6 7 8 9 10 11 12 13 

------------------------------------------------------------------------------------------------------------------

3061 96 75 69 64 63 57 55 51 50 50 47 48 46 43 3062 93 69 69 70 62 63 59 57 51 53 50 51 47 45 3063 96 81 76 70 63 64 57 56 51 52 51 49 47 44 

3064 100 80 73 70 66 62 57 57 54 55 50 49 47 42 3065 94 83 75 70 65 61 61 59 54 53 53 51 48 45 

MEAN 96 78 73 69 64 62 58 56 52 52 50 50 47 44 

S.D. 2.7 5.7 3.3 2.6 1.7 2.5 2.1 3.0 1.6 1.8 2.0 1.3 0.6 1.2 

N 5 5 5 5 5 5 5 5 5 5 5 5 5 5 

P
26-SEP-2003 14:10 

Huntingdon Life Sciences 00-6130N 

Neurotoxicity Sub-Group 

MALES GROUP 4 

ANIMAL# 

20000 MG/M3 

WEEK OF STUDY 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

0 1 2 3 4 5 6 7 8 9 10 11 12 13 

------------------------------------------------------------------------------------------------------------------

4071 93 76 67 62 59 60 58 57 53 53 so 49 48 45 4072 95 76 67 62 60 58 57 53 50 49 49 46 43 41 4073 104 82 73 69 62 61 59 57 so 51 52 51 49 45 4074 93 79 68 69 64 65 64 56 59 52 62 48 48 46 4075 93 83 77 69 66 67 56 55 53 55 51 so 49 46 

MEAN 96 79 71 66 62 62 59 55 53 52 53 49 48 45 

S.D. 4.5 3.2 4.4 3.7 2.9 3.5 3.1 1.6 3.4 2.0 5.1 1.7 2.5 2.2 

N 5 5 5 5 5 5 5 5 5 5 5 5 5 5 

P
26-SEP-2003 14:10 

Huntingdon Life Sciences 00-6130N 

Neurotoxicity Sub-Group 

FEMALES GROUP 1 0 MG/M3 

WEEK OF STUDY 

ANIMAL# 0 1 

1571 102 79 1572 99 87 

1573 101 82 1574 94 82 1575 95 87 

MEAN 98 84 

S.D. 3.4 3.5 

N 5 5 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

2 3 4 5 6 7 8 9 10 11 

76 74 68 69 62 62 54 57 57 57 

82 75 69 72 64 60 61 63 62 58 

80 72 68 63 65 63 56 57 55 56 

77 69 68 71 63 62 58 60 57 59 

81 79 67 66 66 67 59 62 62 63 

79 74 68 68 64 63 58 60 59 58 

2.5 4.0 0.8 3.4 1.6 2.8 2.8 3.0 3.0 3.0 

5 5 5 5 5 5 5 5 5 5 

12 13 

55 47 57 52 

58 47 58 51 

60 57 

58 51 

1.8 4.3 

5 5 

P
26-SEP-2003 14:10 

Huntingdon Life Sciences 00-6130N 

Neurotoxicity Sub-Group 

FEMALES GROUP 2 2000 MG/M3 

WEEK OF STUDY 

ANIMAL# 0 1 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

2 3 4 5 6 7 8 9 10 11 12 13 

------------------------------------------------------------------------------------------------------------------

2561 84 CF 104 71 68 75 67 67 61 70 63 61 64 56 2562 99 100 80 77 64 69 71 65 59 64 66 59 59 51 2563 102 91 74 76 73 70 62 64 62 59 65 61 62 54 2564 89 79 78 70 65 65 63 61 57 59 60 66 57 48 2565 110 88 82 79 68 69 68 68 54 52 61 60 58 so 

MEAN 97 90 84 75 68 69 66 65 59 61 63 61 60 52 

S.D. 10.6 8.4 11.7 4.0 3.7 3.6 3.5 2.8 3.4 6.7 2.5 3.0 3.2 3.2 

N 5 4 5 5 5 5 5 5 5 5 5 5 5 5 

CF=Contaminated Feeder 

P
26-SEP-2003 14:10 

Huntingdon Life Sciences 00-6130N 

Neuro'toxicity Sub-Group 

FEMALES GROUP 3 10000 MG/M3 

WEEK OF STUDY 

ANIMAL# 0 1 

3561 88 72 3562 102 79 3563 90 81 3564 92 76 3565 106 87 

MEAN 96 79 

S.D. 7.7 5.8 

N 5 5 

SF=Spi1led Feeder 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

2 3 4 5 6 7 8 9 10 11 

78 70 65 67 SF 62 59 59 62 58 74 74 71 68 63 64 63 62 61 62 

75 73 71 63 66 64 59 60 59 59 

68 66 65 62 64 62 58 59 59 55 

SF 73 70 67 68 63 58 57 55 53 

74 71 68 65 65 63 59 59 59 57 

4.1 3.5 3.2 2.6 2.3 1.1 2.0 1.8 2.5 3.3 

4 5 5 5 4 5 5 5 5 5 

12 13 

55 53 

62 52 

60 53 

54 53 

55 48 57 52 

3.7 2.2 

5 5 

P
26-SEP-2003 14:10 

Huntingdon Life Sciences 00-6130N 

Neurotoxicity Sub-Group 

FEMALES GROUP 4 

ANIMAL# 

4571 4572 

4573 4574 

4575 

MEAN 

S.D. 

N 

20000 MG/M3 

WEEK OF STUDY 

APPENDIX F 

GASOLINE DIPE VAPOR CONDENSATE: A 13-WEEK WHOLE-BODY 

INHALATION TOXICITY STUDY IN RATS WITH NEUROTOXICITY ASSESSMENTS 

AND 4-WEEK IN VIVO GENOTOXICITY AND IMMUNOTOXICITY ASSESSMENTS 

INDIVIDUAL FEED CONSUMPTION VALUES (GRAMS/KG/DAY) 

0 1 2 3 4 5 6 7 8 9 10 11 

86 78 69 68 68 68 61 66 59 60 58 58 

102 89 78 75 70 70 69 68 62 65 65 66 

93 79 77 77 75 68 71 65 63 59 66 62 

106 88 85 80 70 69 71 71 64 63 64 65 118 88 87 81 74 77 76 71 69 69 65 60 101 84 79 76 71 70 69 68 63 63 64 62 

12.2 5.5 7.0 5.3 2.9 3.5 5.6 2.4 3.5 4.2 3.6 3.5 

5 5 5 5 5 5 5 5 5 5 5 5 

12 1

57 5

66 5

62 5

58 5

63 561 5

3.8 3.0 

5 

P
Huntingdon Life Sciences 00-6130 Page 419

211-DIPE-S Final Report

Males

Individual Motor Activity Valuesa

Pretest Appendix G

a

Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group I - 0 mg/m3

1071 197 204 98 132 127 162 156 110 127 74 5 0

1072 49 131 133 65 63 45 48 10 0 0 0 0

1073 207 186 178 141 113 108 99 52 105 83 1 79

1074 20 75 208 147 134 108 29 60 0 0 0 0

1075 223 133 129 99 146 79 33 164 141 24 16 0

1076 173 150 231 202 44 85 51 5 0 0 2 1

1077 1 150 176 83 116 86 25 0 0 3 0 0

1078 27 167 132 179 151 152 99 132 78 45 0 0

1079 116 133 136 102 64 100 132 74 72 133 146 119

1080 149 107 72 129 136 130 155 87 56 35 40 31

Mean 116.2 143.6 149.3 127.9 109.4 105.5 82.7 69.4 57.9 39.7 21.0 23.0

SD 85.30 37.27 48.78 42.34 38.36 35.21 52.13 55.51 55.69 44.88 45.69 42.26

N 10 10 10 10 10 10 10 10 10 10 10 10

Group II - 2000 mg/m3

2061 174 141 95 21 8 14 7 0 2 1 0 2

2062 146 132 84 119 134 112 59 71 127 31 86 71

2063 256 257 110 113 107 65 59 7 0 1 1 0

2064 137 149 77 25 9 149 97 23 3 0 2 2

2065 175 147 133 216 143 121 49 40 40 0 0 2

2066 207 172 174 120 121 195 175 103 117 16 0 0

2067 11 225 196 147 149 150 57 31 8 0 0 0

2068 72 126 152 155 131 78 173 135 132 86 64 14

2069 19 224 169 160 146 109 68 107 82 17 0 0

2070 1 149 177 142 130 162 126 110 76 8 0 2

Mean 119.8 172.2 136.7 121.8 107.8 115.5 87.0 62.7 58.7 16.0 15.3 9.3

SD 89.14 46.06 42.97 59.72 53.76 52.85 55.21 48.59 54.73 26.68 31.90 22.08

N 10 10 10 10 10 10 10 10 10 10 10 10
Huntingdon Life Sciences 00-6130 Page 420

211-DIPE-S Final Report

Males

Individual Motor Activity Valuesa

Pretest Appendix G

a

Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group III – 10000 mg/m3

3061 147 188 56 111 120 80 0 3 0 0 2 0

3062 49 161 92 146 100 124 31 66 11 2 0 0

3063 127 98 106 119 112 130 20 8 0 9 0 2

3064 137 81 103 73 49 0 0 1 0 0 0 0

3065 22 6 14 52 129 14 6 0 0 0 0 0

3066 193 198 133 141 123 4 12 0 1 2 1 0

3067 209 209 193 202 189 133 112 65 21 5 0 0

3068 232 169 132 164 134 123 152 112 123 30 0 2

3069 157 249 115 94 33 74 153 138 20 55 42 25

3070 94 42 128 89 129 48 82 43 0 0 0 3

Mean 136.7 140.1 107.2 119.1 111.8 73.0 56.8 43.6 17.6 10.3 4.5 3.2

SD 67.30 79.16 47.95 45.12 44.13 53.95 62.41 50.64 38.00 18.19 13.19 7.74

N 10 10 10 10 10 10 10 10 10 10 10 10

Group IV – 20000 mg/m3

4071 19 170 201 81 84 7 13 94 186 142 36 6

4072 36 34 81 116 91 108 108 73 47 0 0 0

4073 11 190 60 4 0 0 0 0 0 6 0 0

4074 136 141 166 182 167 87 13 1 0 0 0 6

4075 20 83 126 125 28 103 112 5 0 0 0 0

4076 161 72 59 112 113 125 24 14 0 0 0 0

4077 138 140 90 123 101 76 66 103 12 0 0 1

4078 228 117 183 130 117 82 30 155 146 70 22 0

4079 225 243 109 187 158 113 122 35 1 0 0 0

4080 235 236 193 156 167 176 28 76 3 0 1 2

Mean 120.9 142.6 126.8 121.6 102.6 87.7 51.6 55.6 39.5 21.8 5.9 1.5

SD 92.68 68.96 55.19 52.49 56.03 52.54 46.45 52.82 68.86 47.55 12.62 2.46

N 10 10 10 10 10 10 10 10 10 10 10 10
Huntingdon Life Sciences 00-6130 Page 421

211-DIPE-S Final Report

Females

Individual Motor Activity Valuesa

Pretest Appendix G

a

Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group I - 0 mg/m3

1571 143 119 67 111 7 0 1 0 0 0 1 0

1572 127 128 25 0 0 84 0 2 0 0 0 3

1573 120 106 99 33 19 0 0 0 0 0 0 0

1574 164 137 139 131 74 145 23 0 0 0 0 0

1575 84 95 97 88 63 5 1 3 4 4 9 0

1576 27 167 132 179 151 152 99 132 78 45 0 0

1577 122 100 101 35 55 66 88 97 59 10 0 0

1578 116 113 89 103 85 100 103 70 69 151 44 88

1579 162 80 141 117 126 88 37 0 0 0 0 0

1580 126 81 108 99 84 67 84 68 49 128 121 48

Mean 119.1 112.6 99.8 89.6 66.4 70.7 43.6 37.2 25.9 33.8 17.5 13.9

SD 39.82 26.68 35.18 53.12 49.16 55.59 44.85 50.04 33.39 57.64 38.87 30.05

N 10 10 10 10 10 10 10 10 10 10 10 10

Group II – 2000 mg/m3

2561 149 154 132 7 0 0 4 71 67 83 1 3

2562 186 142 120 123 124 179 140 139 132 145 57 34

2563 174 156 79 19 109 3 0 18 51 135 89 42

2564 150 121 1 0 0 100 155 132 96 71 88 64

2565 122 79 39 88 40 63 9 0 0 0 0 0

2566 124 126 8 72 167 47 21 18 0 0 0 1

2567 92 93 92 97 68 80 60 47 31 14 6 0

2568 102 54 48 0 0 0 41 130 68 43 0 3

2569 165 159 119 90 112 130 156 113 42 47 178 87

2570 137 113 123 67 80 120 111 5 0 0 0 0

Mean 140.1 119.7 76.1 56.3 70.0 72.2 69.7 67.3 48.7 53.8 41.9 23.4

SD 30.58 35.45 49.21 45.67 58.99 61.28 64.57 56.81 43.92 54.25 60.63 31.85

N 10 10 10 10 10 10 10 10 10 10 10 10
Huntingdon Life Sciences 00-6130 Page 422

211-DIPE-S Final Report

Females

Individual Motor Activity Valuesa

Pretest Appendix G

a

Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group III – 10000 mg/m3

3561 164 208 80 27 44 77 0 0 0 0 0 0

3562 121 130 78 0 0 0 0 0 0 0 0 34

3563 128 90 6 0 4 1 0 0 4 0 0 1

3564 185 77 34 13 0 0 0 0 0 0 0 0

3565 162 153 116 70 179 167 168 58 8 0 0 0

3566 70 105 110 62 73 124 94 79 100 34 16 0

3567 156 73 70 70 0 0 6 70 137 126 103 81

3568 123 108 110 85 32 101 48 94 100 79 44 10

3569 119 92 132 82 74 65 82 7 8 0 0 0

3570 57 133 62 17 4 76 143 119 134 0 0 0

Mean 128.5 116.9 79.8 42.6 41.0 61.1 54.1 42.7 49.1 23.9 16.3 12.6

SD 40.88 40.98 39.19 34.37 56.79 59.69 64.38 46.33 60.33 44.17 33.57 26.31

N 10 10 10 10 10 10 10 10 10 10 10 10

Group IV – 20000 mg/m3

4571 195 142 41 130 79 109 3 0 1 0 1 1

4572 76 128 90 56 26 64 25 0 0 0 0 2

4573 77 36 1 3 0 99 98 41 46 175 27 0

4574 181 100 49 87 102 24 3 0 0 0 0 0

4575 99 141 114 110 127 117 158 125 137 125 88 39

4576 160 114 96 98 91 78 60 70 58 90 61 80

4577 186 149 138 63 94 29 65 68 0 29 94 10

4578 86 126 98 69 92 58 74 46 48 66 69 71

4579 53 122 113 122 101 85 82 116 81 0 0 33

4580 116 126 89 64 128 22 84 24 134 163 106 22

Mean 122.9 118.4 82.9 80.2 84.0 68.5 65.2 49.0 50.5 64.8 44.6 25.8

SD 52.82 32.27 40.84 37.58 40.90 35.15 46.88 45.91 53.30 70.02 43.64 29.78

N 10 10 10 10 10 10 10 10 10 10 10 10
Huntingdon Life Sciences 00-6130 Page 423

211-DIPE-S Final Report

Males

Individual Motor Activity Valuesa

4th week of exposure Appendix G

a

Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group I - 0 mg/m3

1071 180 145 129 187 241 179 105 77 133 166 81 5

1072 183 212 163 194 37 1 0 0 0 0 0 0

1073 263 258 108 204 152 115 174 171 11 46 113 5

1074 176 121 123 54 105 55 7 4 0 0 0 0

1075 186 157 216 141 110 93 69 46 58 98 65 0

1076 179 144 61 151 93 63 73 22 1 0 1 0

1077 216 107 135 100 147 42 37 117 31 45 0 2

1078 197 179 105 125 33 6 1 4 0 0 0 0

1079 214 209 148 128 55 87 20 2 1 1 15 0

1080 142 142 172 114 126 143 96 123 135 13 1 1

Mean 193.6 167.4 136.0 139.8 109.9 78.4 58.2 56.6 37.0 36.9 27.6 1.3

SD 32.12 46.75 42.37 46.41 62.49 57.02 56.33 61.99 54.44 55.63 42.38 2.06

N 10 10 10 10 10 10 10 10 10 10 10 10

Group II – 2000 mg/m3

2061 139 122 148 102 22 42 10 0 20 4 1 0

2062 166 126 142 143 119 113 156 101 182 139 182 141

2063 248 190 58 6 2 33 32 10 1 0 11 0

2064 185 131 101 81 143 53 11 32 0 1 2 0

2065 241 221 151 216 184 167 110 15 0 2 10 8

2066 202 147 58 1 33 0 0 2 0 0 0 0

2067 121 41 117 0 0 0 12 2 10 2 7 5

2068 182 129 110 99 232 151 68 0 14 0 0 0

2069 175 178 107 89 12 0 21 3 0 6 0 3

2070 201 164 121 127 107 161 42 23 1 11 0 2

Mean 186.0 144.9 111.3 86.4 85.4 72.0 46.2 18.8 22.8 16.5 21.3 15.9

SD 39.89 48.78 33.00 69.37 83.35 69.28 50.89 30.84 56.40 43.18 56.63 44.04

N 10 10 10 10 10 10 10 10 10 10 10 10
Huntingdon Life Sciences 00-6130 Page 424

211-DIPE-S Final Report

Males

Individual Motor Activity Valuesa

4th week of exposure Appendix G

a

Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group III – 10000 mg/m3

3061 138 146 142 46 22 0 1 0 1 0 2 1

3062 176 181 167 78 16 17 206 116 87 22 13 26

3063 320 238 137 158 66 173 9 0 0 0 0 0

3064 109 174 134 115 90 221 48 0 1 0 0 0

3065 85 174 98 60 0 0 0 0 0 4 4 3

3066 104 162 119 150 69 38 6 5 0 0 0 0

3067 197 135 205 108 151 196 104 35 17 0 4 0

3068 302 187 154 131 129 114 27 159 22 0 1 9

3069 117 159 63 24 14 10 10 2 56 110 0 0

 32 128 151 42 117 75 22 0 0 0 0 0

Mean 158.0 168.4 137.0 91.2 67.4 84.4 43.3 31.7 18.4 13.6 2.4 3.9

SD 92.75 31.25 38.53 47.70 53.60 85.90 65.18 57.68 29.99 34.56 4.06 8.27

N 10 10 10 10 10 10 10 10 10 10 10 10

Group IV – 20000 mg/m3

4071 45 158 63 98 60 106 58 4 11 4 0 0

4072 90 127 61 137 3 4 0 0 6 0 0 0

4073 119 185 139 52 0 0 0 0 24 0 0 0

4074 232 195 167 116 107 85 179 34 5 5 9 0

4075 124 175 49 145 4 1 0 0 0 0 0 0

4076 229 191 220 184 139 90 7 0 8 0 0 0

4077 196 206 128 166 185 133 82 69 111 32 49 4

4078 174 132 118 93 77 79 96 105 96 24 42 2

4079 284 190 176 70 148 121 15 2 4 6 8 0

4080 177 235 153 26 6 5 0 0 0 0 0 2

Mean 167.0 179.4 127.4 108.7 72.9 62.4 43.7 21.4 26.5 7.1 10.8 0.8

SD 72.81 32.98 55.86 50.59 69.40 53.99 60.11 37.09 41.30 11.42 18.69 1.40

N 10 10 10 10 10 10 10 10 10 10 10 10
Huntingdon Life Sciences 00-6130 Page 425

211-DIPE-S Final Report

Females

Individual Motor Activity Valuesa

4th week of exposure Appendix G

a

Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group I - 0 mg/m3

1571 120 100 5 0 0 0 0 0 0 0 0 0

1572 131 81 49 0 0 0 0 0 0 0 0 1

1573 132 94 52 109 55 30 21 14 0 0 0 0

1574 98 119 106 109 102 84 108 29 34 51 21 4

1575 165 66 37 0 0 0 0 0 0 4 0 0

1576 55 99 82 46 102 103 102 75 53 3 0 0

1577 119 142 102 105 76 35 29 17 0 0 0 0

1578 138 133 85 106 119 103 132 118 53 5 23 0

1579 171 189 111 145 8 48 44 131 64 0 0 0

1580 129 137 88 127 95 85 2 9 3 0 0 0

Mean 125.8 116.0 71.7 74.7 55.7 48.8 43.8 39.3 20.7 6.3 4.4 0.5

SD 32.78 35.74 34.55 57.24 49.25 42.31 51.13 50.19 27.07 15.83 9.29 1.27

N 10 10 10 10 10 10 10 10 10 10 10 10

Group II – 2000 mg/m3

2561 154 111 133 145 121 74 18 12 0 0 0 0

2562 132 102 87 138 90 110 50 23 9 0 0 0

2563 83 104 93 56 28 10 0 7 3 0 0 0

2564 115 158 83 45 90 211 93 73 83 34 53 11

2565 143 127 100 107 87 69 0 3 4 0 1 0

2566 139 128 167 76 18 4 0 0 0 0 0 0

2567 108 100 87 97 2 41 11 0 2 5 0 9

2568 159 138 80 22 0 0 0 0 0 0 0 0

2569 161 129 22 0 105 7 1 0 0 0 0 1

2570 145 110 102 108 28 11 0 3 0 0 0 0

Mean 133.9 120.7 95.4 79.4 56.9 53.7 17.3 12.1 10.1 3.9 5.4 2.1

SD 25.00 18.61 37.31 48.27 45.89 66.66 30.92 22.62 25.77 10.69 16.73 4.20

N 10 10 10 10 10 10 10 10 10 10 10 10
Huntingdon Life Sciences 00-6130 Page 426

211-DIPE-S Final Report

Females

Individual Motor Activity Valuesa

4th week of exposure Appendix G

a

Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group III – 10000 mg/m3

3561 138 41 0 0 0 74 19 0 0 2 0 0

3562 182 121 77 4 0 0 0 0 0 0 5 0

3563 121 167 75 85 70 81 9 3 4 0 0 0

3564 137 78 5 14 5 0 0 0 0 0 2 0

3565 165 234 176 175 109 76 131 94 6 0 0 0

3566 155 144 123 83 22 9 0 0 0 0 0 0

3567 123 77 106 94 27 15 0 0 0 0 0 0

3568 120 128 81 75 0 34 0 0 0 0 0 0

3569 111 102 103 86 8 21 14 6 0 1 0 0

3570 107 107 43 5 1 16 98 61 5 0 0 26

Mean 135.9 119.9 78.9 62.1 24.2 32.6 27.1 16.4 1.5 0.3 0.7 2.6

SD 24.58 53.95 53.42 56.04 36.86 32.22 47.21 33.19 2.46 0.67 1.64 8.22

N 10 10 10 10 10 10 10 10 10 10 10 10

Group IV – 20000 mg/m3

4571 54 126 87 0 0 0 0 0 10 6 0 1

4572 144 75 106 44 9 0 0 0 0 104 116 82

4573 38 168 134 83 87 63 4 12 0 0 0 0

4574 133 82 95 29 49 0 0 0 0 0 0 0

4575 215 192 174 113 130 146 78 59 138 146 17 74

4576 148 142 146 16 97 134 119 15 44 0 0 0

4577 138 100 92 100 76 98 44 94 110 0 0 0

4578 139 76 84 24 10 64 106 55 70 66 34 21

4579 111 101 96 118 96 92 49 27 1 0 0 3

4580 153 138 120 109 73 21 146 134 108 134 69 2

Mean 127.3 120.0 113.4 63.6 62.7 61.8 54.6 39.6 48.1 45.6 23.6 18.3

SD 50.50 40.14 29.66 45.49 44.06 55.43 55.11 45.60 54.37 61.18 39.57 32.16

N 10 10 10 10 10 10 10 10 10 10 10 10
Huntingdon Life Sciences 00-6130 Page 427

211-DIPE-S Final Report

Males

Individual Motor Activity Valuesa

8th week of exposure Appendix G

a

Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group I - 0 mg/m3

1071 153 104 154 61 54 68 18 2 2 13 8 23

1072 161 3 2 5 0 0 1 0 0 0 1 0

1073 210 228 178 143 134 72 53 10 0 71 174 145

1074 156 92 56 0 3 0 0 0 0 0 0 0

1075 137 78 84 11 0 0 0 0 0 0 0 17

1076 153 113 31 70 0 5 3 8 0 8 0 2

1077 178 194 111 107 87 79 156 71 9 23 22 12

1078 148 182 75 87 29 2 0 0 0 0 0 0

1079 105 136 68 106 160 29 15 33 0 0 0 0

1080 190 87 33 127 91 164 21 29 1 0 0 0

Mean 159.1 121.7 79.2 71.7 55.8 41.9 26.7 15.3 1.2 11.5 20.5 19.9

SD 28.92 65.69 55.27 51.84 59.66 54.02 48.32 23.09 2.82 22.31 54.39 44.76

N 10 10 10 10 10 10 10 10 10 10 10 10

Group II – 2000 mg/m3

2061 118 137 40 10 0 6 59 2 0 4 0 0

2062 154 134 102 40 1 0 10 0 0 30 128 124

2063 151 62 25 76 7 0 1 0 0 0 0 0

2064 219 67 23 79 113 100 20 0 7 38 12 0

2065 152 154 117 104 91 118 49 4 13 0 10 1

2066 166 168 71 0 14 0 0 2 4 6 5 0

2067 186 196 19 62 5 0 38 0 0 0 0 3

2068 181 103 103 57 19 0 2 1 6 0 7 17

2069 165 155 57 0 3 1 0 0 0 1 5 2

2070 16 23 0 2 2 5 11 3 46 30 42 60

Mean 150.8 119.9 55.7 43.0 25.5 23.0 19.0 1.2 7.6 10.9 20.9 20.7

SD 54.26 54.59 40.94 38.21 41.09 45.58 21.96 1.48 14.18 15.31 39.61 40.82

N 10 10 10 10 10 10 10 10 10 10 10 10
Huntingdon Life Sciences 00-6130 Page 428

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Males

Individual Motor Activity Valuesa

8th week of exposure Appendix G

a

Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group III – 10000 mg/m3

3061 103 61 12 0 59 137 13 0 2 5 2 2

3062 135 14 63 16 56 41 30 15 4 0 0 1

3063 155 58 30 2 0 0 0 0 10 0 0 4

3064 171 71 26 2 0 0 0 2 4 1 10 0

3065 132 58 38 4 6 4 10 0 0 0 6 0

3066 222 205 183 98 84 7 1 9 0 0 0 5

3067 253 236 69 78 161 58 39 1 28 4 0 6

3068 253 236 69 78 161 58 39 1 28 4 0 6

3069 102 8 0 8 4 0 1 1 0 0 0 4

3070 144 45 83 47 27 23 0 0 0 0 0 0

Mean 167.0 99.2 57.3 33.3 55.8 32.8 13.3 2.9 7.6 1.4 1.8 2.8

SD 56.84 89.93 51.85 38.35 62.50 43.48 16.48 5.04 11.19 2.07 3.46 2.49

N 10 10 10 10 10 10 10 10 10 10 10 10

Group IV – 20000 mg/m3

4071 146 41 56 138 37 27 54 7 0 2 5 2

4072 200 119 22 41 1 0 0 0 0 9 0 12

4073 80 84 15 18 0 3 0 0 0 5 0 0

4074 109 79 102 55 93 6 1 0 0 0 2 0

4075 152 91 78 26 50 71 94 0 4 5 3 1

4076 145 154 75 50 123 150 73 176 145 34 9 42

4077 227 126 126 158 57 6 90 143 50 7 136 56

4078 75 84 105 35 69 38 1 0 12 4 6 4

4079 156 95 129 14 4 3 93 152 131 113 20 0

4080 263 205 127 33 6 72 1 0 0 0 0 0

Mean 155.3 107.8 83.5 56.8 44.0 37.6 40.7 47.8 34.2 17.9 18.1 11.7

SD 60.74 45.84 42.04 49.96 42.62 48.13 43.80 75.81 56.91 34.83 41.87 20.27

N 10 10 10 10 10 10 10 10 10 10 10 10
Huntingdon Life Sciences 00-6130 Page 429

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Females

Individual Motor Activity Valuesa

8th week of exposure Appendix G

a

Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group I - 0 mg/m3

1571 138 100 28 71 30 35 9 4 0 0 0 0

1572 57 0 0 0 0 0 0 1 9 0 0 1

1573 159 113 128 55 20 33 0 0 0 0 0 0

1574 148 139 57 94 1 0 0 0 0 0 11 80

1575 71 95 59 81 79 64 97 32 23 2 9 5

1576 134 122 148 56 24 1 1 0 0 0 0 0

1577 149 180 129 128 85 104 86 64 79 81 40 5

1578 161 132 107 128 76 108 22 0 0 0 0 0

1579 159 132 75 22 120 19 0 40 3 0 0 1

1580 163 110 151 87 90 124 30 0 12 1 0 0

Mean 133.9 112.3 88.2 72.2 52.5 48.8 24.5 14.1 12.6 8.4 6.0 9.2

SD 38.23 46.23 52.20 41.22 42.24 48.09 36.92 22.97 24.53 25.52 12.66 24.96

N 10 10 10 10 10 10 10 10 10 10 10 10

Group II – 2000 mg/m3

2561 117 55 55 63 43 0 1 0 0 0 5 0

2562 99 91 47 0 36 0 0 0 0 0 0 0

2563 88 108 30 0 0 0 0 0 0 0 14 2

2564 138 76 64 13 0 69 42 60 10 0 0 38

2565 122 79 95 76 127 86 77 130 77 87 30 4

2566 187 99 60 2 65 9 0 0 0 0 0 0

2567 128 29 61 17 3 0 0 3 0 0 0 0

2568 111 69 77 88 29 15 1 0 0 0 0 0

2569 85 3 117 38 0 0 0 0 0 0 0 0

2570 160 126 120 2 0 0 0 0 0 0 0 0

Mean 123.5 73.5 72.6 29.9 30.3 17.9 12.1 19.3 8.7 8.7 4.9 4.4

SD 31.87 36.90 29.60 34.09 41.05 32.07 26.31 43.18 24.20 27.51 9.89 11.88

N 10 10 10 10 10 10 10 10 10 10 10 10
Huntingdon Life Sciences 00-6130 Page 430

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Females

Individual Motor Activity Valuesa

8th week of exposure Appendix G

a

Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group III – 10000 mg/m3

3561 100 79 47 7 0 1 0 0 90 13 12 5

3562 78 65 0 1 0 0 0 0 0 46 3 0

3563 96 87 64 2 2 0 0 1 3 1 0 0

3564 81 111 6 10 11 0 0 1 2 6 0 0

3565 193 71 96 53 23 0 0 2 2 14 2 0

3566 155 56 119 1 1 0 0 0 7 0 0 0

3567 190 97 131 17 0 0 0 4 1 10 0 47

3568 134 21 0 0 0 0 0 0 0 0 0 1

3569 108 72 4 0 0 0 0 0 0 1 6 0

3570 104 13 0 0 0 0 0 5 0 0 0 0

Mean 123.9 67.2 46.7 9.1 3.7 0.1 0.0 1.3 10.5 9.1 2.3 5.3

SD 42.37 30.87 52.80 16.41 7.59 0.32 0.00 1.83 28.02 14.11 3.95 14.74

N 10 10 10 10 10 10 10 10 10 10 10 10

Group IV – 20000 mg/m3

4571 110 67 4 71 3 0 29 4 0 1 5 0

4572 97 82 68 34 35 68 0 6 0 0 5 27

4573 69 93 44 1 0 0 5 0 0 0 11 0

4574 96 46 0 0 0 0 0 0 0 0 7 0

4575 160 123 92 31 37 1 0 1 0 0 2 0

4576 198 149 123 91 41 33 22 0 0 0 0 0

4577 182 172 168 84 0 0 8 3 0 0 0 0

4578 117 132 96 1 28 109 110 107 86 59 40 8

4579 136 141 84 84 113 18 5 0 0 0 0 0

4580 198 38 74 5 1 70 18 0 0 0 0 1

Mean 136.3 104.3 75.3 40.2 25.8 29.9 19.7 12.1 8.6 6.0 7.0 3.6

SD 45.98 45.77 51.03 38.61 35.25 39.27 33.30 33.41 27.20 18.62 12.17 8.59

N 10 10 10 10 10 10 10 10 10 10 10 10
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Males

Individual Motor Activity Valuesa

13th week of exposure Appendix G

a

Number of beam breaks per interval

b

Sacrificed for humane reason.

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group I - 0 mg/m3

1071 233 150 102 17 45 26 2 0 15 40 38 0

1072 215 30 71 141 32 5 9 6 7 7 0 0

1073 220 121 124 30 46 13 8 0 5 117 94 20

1074 225 122 64 15 2 0 7 12 0 2 1 0

1075 156 132 161 80 79 38 7 107 7 0 0 3

1076 67 111 71 53 87 0 2 6 0 0 0 3

1077 154 161 107 70 96 155 28 8 156 103 64 11

1078 253 158 130 42 21 8 25 2 0 47 115 1

1079 137 148 131 77 24 40 0 0 5 0 17 54

1080 163 198 121 9 27 90 26 34 59 57 7 4

Mean 182.3 133.1 108.2 53.4 45.9 37.5 11.4 17.5 25.4 37.3 33.6 9.6

SD 56.86 44.10 31.55 40.36 31.40 49.49 10.73 33.04 49.15 44.09 43.01 16.83

N 10 10 10 10 10 10 10 10 10 10 10 10

Group II – 2000 mg/m3

2061 237 130 26 8 7 34 0 0 0 2 0 1

2062 164 90 83 11 40 1 0 3 0 0 1 0

2063 197 167 17 6 0 1 0 0 0 0 3 5

2064 232 164 146 111 46 19 11 0 0 0 0 0

2065 197 135 142 105 56 77 64 75 118 86 4 143

2066 216 88 64 91 30 56 177 51 100 2 13 0

2067 159 102 54 8 8 14 52 98 1 4 19 9

2068 192 108 169 69 39 12 0 0 5 0 1 4

2069 164 95 5 0 4 0 0 0 1 1 2 3

2070 b b b b b b b b b b b b

Mean 195.3 119.9 78.4 45.4 25.6 23.8 33.8 25.2 25.0 10.6 4.8 18.3

SD 29.17 30.59 60.78 47.55 20.99 27.01 59.14 38.91 47.86 28.32 6.67 46.85

N 9 9 9 9 9 9 9 9 9 9 9 9
Huntingdon Life Sciences 00-6130 Page 432

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Males

Individual Motor Activity Valuesa

13th week of exposure Appendix G

a

Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group III – 10000 mg/m3

3061 88 37 11 0 7 0 0 9 4 53 119 5

3062 87 136 46 108 27 65 10 13 40 139 36 4

3063 229 78 46 5 1 0 0 125 0 0 0 0

3064 79 140 67 45 116 115 9 0 7 0 1 15

3065 140 25 40 2 0 0 0 0 0 0 0 0

3066 205 151 127 112 16 2 32 4 0 8 0 0

3067 241 209 150 2 0 3 4 3 0 0 0 2

3068 196 106 72 101 75 48 0 21 0 0 40 22

3069 160 167 136 7 0 34 92 1 4 0 13 1

3070 198 96 23 0 15 55 14 75 23 1 0 14

Mean 162.3 114.5 71.8 38.2 25.7 32.2 16.1 25.1 7.8 20.1 20.9 6.3

SD 61.02 57.63 49.13 49.36 39.07 38.84 28.46 41.72 13.34 44.91 37.80 7.85

N 10 10 10 10 10 10 10 10 10 10 10 10

Group IV – 20000 mg/m3

4071 219 119 59 74 0 15 0 15 133 17 33 0

4072 137 111 8 16 31 75 2 10 1 6 0 0

4073 150 74 116 0 6 1 1 2 2 1 2 4

4074 123 114 116 104 63 14 8 11 6 0 0 1

4075 174 70 77 118 63 11 46 0 1 0 0 0

4076 138 91 57 18 23 17 3 49 90 98 5 1

4077 172 126 100 111 23 8 21 107 41 3 8 6

4078 158 120 99 90 199 138 24 10 47 2 0 0

4079 210 75 8 11 74 5 0 0 6 0 0 0

4080 254 127 89 0 0 52 0 0 0 0 0 0

Mean 173.5 102.7 72.9 54.2 48.2 33.6 10.5 20.4 32.7 12.7 4.8 1.2

SD 41.93 22.83 39.83 49.40 59.48 43.47 15.31 33.73 46.02 30.42 10.28 2.10

N 10 10 10 10 10 10 10 10 10 10 10 10
Huntingdon Life Sciences 00-6130 Page 433

211-DIPE-S Final Report

Females

Individual Motor Activity Valuesa

13th week of exposure Appendix G

a

Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group I - 0 mg/m3

1571 83 128 119 119 41 1 8 82 9 82 41 1

1572 87 14 0 0 0 0 0 3 0 2 0 1

1573 185 102 91 44 0 3 4 0 0 4 27 13

1574 159 140 99 105 111 14 9 7 35 1 2 0

1575 93 61 78 96 55 51 37 0 23 3 3 0

1576 139 104 112 48 53 0 0 4 37 91 65 25

1577 144 123 99 59 120 87 72 44 12 0 0 1

1578 157 136 38 31 0 39 9 0 1 0 3 0

1579 208 105 141 141 6 0 0 0 0 0 3 0

1580 120 122 118 8 0 31 6 0 2 0 7 0

Mean 137.5 103.5 89.5 65.1 38.6 22.6 14.5 14.0 11.9 18.3 15.1 4.1

SD 42.06 38.78 41.92 47.90 46.39 29.35 22.92 27.43 14.67 36.03 22.20 8.36

N 10 10 10 10 10 10 10 10 10 10 10 10

Group II – 2000 mg/m3

2561 120 80 89 47 3 36 0 0 9 93 37 0

2562 148 84 78 54 113 28 2 0 1 9 0 0

2563 117 92 91 5 1 0 0 13 6 5 4 0

2564 127 49 18 7 19 1 5 28 11 0 0 5

2565 164 55 88 1 1 0 0 0 67 98 124 64

2566 171 56 21 2 2 0 0 1 0 0 9 2

2567 119 58 37 14 2 0 0 0 0 0 0 0

2568 146 51 109 77 1 4 152 134 140 135 50 0

2569 196 95 0 31 1 3 80 48 0 0 0 0

2570 92 63 0 0 0 0 0 6 0 0 1 1

Mean 140.0 68.3 53.1 23.8 14.3 7.2 23.9 23.0 23.4 34.0 22.5 7.2

SD 30.94 17.61 41.96 27.13 35.13 13.28 51.45 42.11 45.77 52.73 39.83 20.02

N 10 10 10 10 10 10 10 10 10 10 10 10
Huntingdon Life Sciences 00-6130 Page 434

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Females

Individual Motor Activity Valuesa

13th week of exposure Appendix G

a

Number of beam breaks per interval

Animal Interval

Number 1 2 3 4 5 6 7 8 9 10 11 12

Group III – 10000 mg/m3

3561 184 100 122 88 21 0 0 3 7 7 6 2

3562 131 66 21 0 58 3 4 69 8 90 5 0

3563 139 81 69 76 0 0 0 0 0 0 1 0

3564 146 53 3 0 0 0 0 0 0 0 1 1

3565 54 62 2 0 15 125 101 40 30 10 0 1

3566 142 97 15 104 38 97 26 0 0 0 0 0

3567 188 164 99 144 20 8 0 3 0 0 1 6

3568 101 47 1 0 0 1 1 0 5 34 133 28

3569 97 135 136 74 0 0 0 0 27 0 8 2

3570 106 69 62 0 4 0 3 0 0 0 3 0

Mean 128.8 87.4 53.0 48.6 15.6 23.4 13.5 11.5 7.7 14.1 15.8 4.0

SD 40.87 37.54 52.04 54.70 19.60 46.71 31.76 23.70 11.42 28.71 41.27 8.63

N 10 10 10 10 10 10 10 10 10 10 10 10

Group IV – 20000 mg/m3

4571 149 102 25 21 52 0 0 0 2 3 0 13

4572 98 92 71 21 82 0 0 0 0 0 0 0

4573 122 27 25 0 0 0 0 0 1 0 0 0

4574 146 69 4 20 4 3 1 1 2 2 1 4

4575 219 155 152 100 73 89 9 1 0 0 1 1

4576 145 117 106 29 69 31 23 23 1 0 15 8

4577 122 89 113 10 0 0 2 6 0 0 0 0

4578 129 103 57 87 40 71 9 63 74 19 0 0

4579 189 133 133 145 69 3 0 2 153 0 0 1

4580 162 75 22 0 31 0 0 0 0 0 0 0

Mean 148.1 96.2 70.8 43.3 42.0 19.7 4.4 9.6 23.3 2.4 1.7 2.7

SD 35.19 35.55 52.40 49.52 32.00 33.43 7.47 20.05 51.06 5.93 4.69 4.45

N 10 10 10 10 10 10 10 10 10 10 10 10
Huntingdon Life Sciences 00-6130 Page 435

211-DIPE-S Final Report

Individual Functional Observational 

Battery Evaluations 

Preface Appendix H

Key:

HOME CAGE EVALUATIONS

Posture: 1 - Sitting or standing normally

(Post) 2 - Alert; sitting or standing oriented 

 toward the observer

3 - Asleep; may be curled up

4 - Lying on side

5 - Flattened; limbs may be spread

6 - Crouched; sitting hunched, head

hung down

Vocalizations: 1 - No vocalization present

(Vocal) 2 - Vocalization present

Palpebral Closure: 1 - Eyelids open

(Palp Close) 2 - Eyelids completely closed

3 - Eyelids slightly drooping

4 - Eyelids half closed

HANDLING EVALUATIONS

Ease of Removal: 1 - Very easy; sits quietly, accepts 

(Rem) observer’s touch

2 - Easy; vocalization without resistance

3 - Moderately difficult; rears, often

follows observer’s hand

4 - Freezes; with or without vocalization

5 - Difficult; runs around cage, is hard to

pick up, attempts to bite or attack,

with or without vocalization
Huntingdon Life Sciences 00-6130 Page 436

211-DIPE-S Final Report

Individual Functional Observational 

Battery Evaluations 

Preface Appendix H

HANDLING EVALUATIONS (cont.)

Ease of Handling: 1 - Very easy; totally limp

(Hand) 2 - Normal

3 - Moderately Difficult

 4 - Freezes

5 - Difficult

Chromodacryorrhea: 1 - Not present

(Chromo) 2 - Present

Lacrimation: 1 - No lacrimation

(Lac) 2 - Some lacrimation

3 - Extreme lacrimation

Coat: 1 - Normal; well groomed

2 - Slightly soiled

3 - Moderately soiled

4 - Extremely soiled; crusty unkempt

Salivation: 1 - Not present

(Sal) 2 - Slight salivation; around mouth

3 - Moderate salivation (facial and/or

 cervical area)

4 - Extreme salivation (on ventral

 surface)
Huntingdon Life Sciences 00-6130 Page 437

211-DIPE-S Final Report

Individual Functional Observational 

Battery Evaluations 

Preface Appendix H

OPEN FIELD EVALUATIONS

Gait and Posture: 1 - Normal

(Gait) 2 - Ataxia; uncoordinated movement,

excessive sway, rock or lurch

3 - Body drags or is flattened; animal’s

ventral surface contacts cart surface

4 - Limbs splayed or dragging (indicate hind or fore)

5 - Hunched posture

Locomotion: 1 - Normal (animal moves easily around

(Loco) open field)

2 - Increased movement

 3 - Decreased movement

4 - None, animal does not move around

field

Arousal: 1 - Normal; alert with exploratory

 movements

2 - Low; slight stupor, some head or body

movements

3 - Very low; stupor, little or no

responsiveness to the environment

4 - High; slight excitement, tense, sudden

darting or freezing

5 - Very high; hyperalert, sudden boost of 

running or movement

Piloerection: 1 - Not present

(Pilo) 2 - Present

Exophthalmia: 1 - Not present

(Exo) 2 - Present

Feces: U - Unformed stool

# - Number of pellets indicated

Urine: X - Polyuria

# - Number of pools indicated
Huntingdon Life Sciences 00-6130 Page 438

211-DIPE-S Final Report

Individual Functional Observational 

Battery Evaluations 

Preface Appendix H

REFLEX ASSESSMENTS

Visual Approach: 1 - Normal, slowly approaches, sniffs

 and/or turns away

2 - No reaction

3 - Freezes or pulls away slightly

4 - Jumps or turns abruptly to avoid, or attacks

and/or bites

Pain Perception: 1 - Normal; turns and bites at the site or

(Pain) walks forward, away from the stimulus

 2 - No reaction

 3 - Increased pain; jumps forward, with or

without vocalization, or exhibits highly 

exaggerated bizarre reaction, attacks, bites

Audition Assessment: 1 - Normal; flinches and/or flicks ears

(Sound) 2 - No reaction

3 - Exaggerated; jumps, flips, bites

Pupil Response: 1 - Pupil constricts normally

2 - Pupil size does not change

3 - Miosis (location)

4 - Mydriasis (location)

Proprioception: 1 - Returns leg to original position

(Propri) 2 - Returns leg only partially to original

position

3 - No response, rat allows leg to remain 

pulled back

Pinna Reflex: 1 - Ear flattens against head

2 - Animal shakes head

3 - No response

Air Righting Reflex: 1 - Normal; lands on four feet

2 - Slightly uncoordinated; two-legged or

unsteady landing

3 - Lands on side

4 - Lands on back
Huntingdon Life Sciences 00-6130 Page 439

211-DIPE-S Final Report

Individual Functional Observational 

Battery Evaluations 

Preface Appendix H

ABNORMAL MOVEMENTS

Convulsions: 1 - Not present

(Conv) 2 - Slight

3 - Extreme

Tremors: 1 - Not present

(Trem) 2 - Slight

3 - Extreme

Fasciculation: 1 - Not present

(Fasc) 2 - Slight

3 - Extreme

GRIP STRENGTH

Forelimb and hindlimb grip strength measured in grams of force

LANDING FOOT SPLAY

Measured in centimeters

BODY WEIGHTS

Measured in grams
Huntingdon Life Sciences 00-6130 Page 440

211-DIPE-S Final Report

Males

Individual Functional Observational Battery Evaluations

Pretest Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp 

Number Post Vocal Close Rem Han Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group I – 0 mg/m3

1071 1 1 1 1 2 1 1 1 1 257.0 1 1 1 1 1 0 0 1 1 1

1072 1 1 1 1 2 1 1 1 1 230.9 1 1 1 1 1 0 0 1 1 1

1073 1 1 1 1 2 1 1 1 1 256.1 1 1 1 1 1 0 0 1 1 1

1074 1 1 1 1 1 1 1 1 1 227.7 1 1 1 1 1 0 0 1 1 1

1075 1 1 1 1 1 1 1 1 1 250.0 1 1 1 1 1 0 0 1 1 1

1076 1 1 1 1 2 1 1 1 1 279.6 1 1 1 1 1 3 2 1 1 1

1077 1 1 1 1 2 1 1 1 1 271.2 1 1 1 1 1 0 1 1 1 1

1078 1 1 1 1 2 1 1 1 1 259.7 1 1 1 1 1 6 0 1 1 1

1079 1 1 1 1 2 1 1 1 1 273.1 1 1 1 1 1 0 0 1 1 1

1080 2 1 1 1 2 1 1 1 1 262.2 1 1 1 1 1 0 0 1 1 1

Group II – 2000 mg/m3

2061 1 1 1 1 2 1 1 1 1 226.8 1 1 1 1 1 0 0 1 1 1

2062 1 1 1 1 2 1 1 1 1 273.2 1 1 1 1 1 0 0 1 1 1

2063 1 1 1 1 1 1 1 1 1 257.9 1 1 1 1 1 1 0 1 1 1

2064 1 1 1 1 1 1 1 1 1 217.9 1 1 1 1 1 0 0 1 1 1

2065 1 1 1 1 1 1 1 1 1 266.4 1 1 1 1 1 0 0 1 1 1

2066 1 1 1 1 2 1 1 1 1 277.0 1 1 1 1 1 0 0 1 1 1

2067 1 1 1 1 2 1 1 1 1 233.4 1 1 1 1 1 2 1 1 1 1

2068 1 1 1 1 2 1 1 1 1 285.6 1 1 1 1 1 0 2 1 1 1

2069 1 1 1 1 2 1 1 1 1 271.4 1 1 1 1 1 0 0 1 1 1

2070 1 1 1 2 2 1 1 1 1 255.7 1 1 1 1 1 0 1 1 1 1
Huntingdon Life Sciences 00-6130 Page 441

211-DIPE-S Final Report

Males

Individual Functional Observational Battery Evaluations

Pretest Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group I - 0 mg/m3

1071 1 1 1 1 2 1 790 875 833 715 680 698 1 5.7 5.1 5.4

1072 1 1 1 1 2 1 880 1050 965 695 610 653 1 5.1 4.8 5.0

1073 1 1 1 1 2 1 800 1050 925 660 445 553 1 9.0 7.3 8.2

1074 1 1 1 1 2 1 830 780 805 620 610 615 1 6.5 6.8 6.7

1075 1 1 1 1 2 1 780 980 880 360 410 385 1 7.8 7.4 7.6

1076 1 1 1 1 2 1 565 750 658 560 595 578 1 4.5 4.1 4.3

1077 1 1 1 1 2 1 965 1205 1085 830 715 773 1 8.2 7.4 7.8

1078 1 1 1 1 2 1 945 995 970 490 610 550 1 5.9 5.1 5.5

1079 1 1 1 1 2 1 705 1010 858 550 675 613 1 7.1 7.2 7.2

1080 1 1 1 1 2 1 785 825 805 485 385 435 1 5.5 4.3 4.9

Group II – 2000 mg/m3

2061 1 1 1 1 2 1 850 1000 925 490 565 528 1 7.2 7.1 7.2

2062 1 1 1 1 2 1 855 840 848 560 425 493 1 5.3 5.2 5.3

2063 1 1 1 1 2 1 1005 1245 1125 505 490 498 1 9.8 7.3 8.6

2064 1 1 1 1 2 1 810 960 885 460 410 435 1 4.8 5.2 5.0

2065 1 1 1 1 2 1 880 760 820 430 395 413 1 4.6 4.2 4.4

2066 1 1 1 1 2 1 780 845 813 590 690 640 1 6.0 6.2 6.1

2067 1 1 1 1 2 1 1110 1265 1188 755 690 723 1 7.7 5.4 6.6

2068 1 1 1 1 2 1 1095 980 1038 530 840 685 1 5.9 4.8 5.4

2069 1 1 1 1 2 1 720 1100 910 730 600 665 1 6.2 4.8 5.5

2070 1 1 1 1 2 1 795 1105 950 800 920 860 1 8.1 7.8 8.0
Huntingdon Life Sciences 00-6130 Page 442

211-DIPE-S Final Report

Males

Individual Functional Observational Battery Evaluations

Pretest Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp 

Number Post Vocal Close Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group III – 10000 mg/m3

3061 1 1 1 1 2 1 1 1 1 246.3 1 1 1 1 1 0 1 1 1 1

3062 1 1 1 1 2 1 1 1 1 264.4 1 1 1 1 1 0 6 1 1 1

3063 1 1 1 1 2 1 1 1 1 242.2 1 1 1 1 1 0 0 1 1 1

3064 1 1 1 1 1 1 1 1 1 254.0 1 1 1 1 1 4 4 1 1 1

3065 1 1 1 1 1 1 1 1 1 212.6 1 1 1 1 1 0 0 1 1 1

3066 1 1 1 1 2 1 1 1 1 244.8 1 1 1 1 1 0 0 1 1 1

3067 1 1 1 1 2 1 1 1 1 265.9 1 1 1 1 1 1 2 1 1 1

3068 1 1 1 1 2 1 1 1 1 265.6 1 1 1 1 1 0 0 1 1 1

3069 1 1 1 1 2 1 1 1 1 283.7 1 1 1 1 1 0 0 1 1 1

3070 1 1 1 1 2 1 1 1 1 263.3 1 1 1 1 1 1 4 1 1 1

Group IV – 20000 mg/m3

4071 1 1 1 1 2 1 1 1 1 243.5 1 1 1 1 1 0 1 1 1 1

4072 1 1 1 1 2 1 1 1 1 226.8 1 1 1 1 1 0 0 1 1 1

4073 1 1 1 1 1 1 1 1 1 271.4 1 1 1 1 1 3 2 1 1 1

4074 1 1 1 1 1 1 1 1 1 255.9 1 1 1 1 1 0 2 1 1 1

4075 1 1 1 1 1 1 1 1 1 227.2 1 1 1 1 1 0 0 1 1 1

4076 1 1 1 1 2 1 1 1 1 272.5 1 1 1 1 1 0 0 1 1 1

4077 1 1 1 1 2 1 1 1 1 259.4 1 1 1 1 1 4 2 1 1 1

4078 1 1 1 2 2 1 1 1 1 299.4 1 1 1 1 1 2 1 1 1 1

4079 1 1 1 2 2 1 1 1 1 280.6 1 1 1 1 1 0 0 1 1 1

4080 1 1 1 1 2 1 1 1 1 271.7 1 1 1 1 1 1 0 1 1 1
Huntingdon Life Sciences 00-6130 Page 443

211-DIPE-S Final Report

Males

Individual Functional Observational Battery Evaluations

Pretest Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group III – 10000 mg/m3

3061 1 1 1 1 2 1 535 1065 800 340 625 483 1 5.6 5.1 5.4

3062 1 1 1 1 2 1 955 970 963 590 625 608 1 6.0 4.3 5.2

3063 1 1 1 1 2 1 860 960 910 510 415 463 1 6.3 5.5 5.9

3064 1 1 1 1 2 1 900 1010 955 445 355 400 1 8.3 7.3 7.8

3065 1 1 1 1 2 1 735 1020 878 600 435 518 1 9.9 6.4 8.2

3066 1 1 1 1 2 1 920 875 898 475 720 598 1 7.7 6.7 7.2

3067 1 1 1 1 2 1 850 950 900 665 750 708 1 7.3 5.6 6.5

3068 1 1 1 1 2 1 625 990 808 760 625 693 1 5.9 5.5 5.7

3069 1 1 1 1 2 1 935 1000 968 595 450 523 1 6.6 5.2 5.9

3070 1 1 1 1 2 1 650 755 703 430 550 490 1 5.2 6.1 5.7

Group IV – 20000 mg/m3

4071 1 1 1 1 2 1 870 955 913 485 455 470 1 8.3 8.4 8.4

4072 1 1 1 1 2 1 955 885 920 715 610 663 1 7.7 8.5 8.1

4073 1 1 1 1 2 1 865 940 903 235 640 438 1 6.5 7.2 6.9

4074 1 1 1 1 2 1 1005 1020 1013 875 660 768 1 6.6 6.6 6.6

4075 1 1 1 1 2 1 595 950 773 495 430 463 1 5.2 5.6 5.4

4076 1 1 1 1 2 1 1145 1140 1143 610 660 635 1 6.0 5.7 5.9

4077 1 1 1 1 2 1 960 1040 1000 670 650 660 1 7.0 5.6 6.3

4078 1 1 1 1 2 1 855 1050 953 785 445 615 1 6.4 7.0 6.7

4079 1 1 1 1 2 1 875 1030 953 820 885 853 1 8.3 8.1 8.2

4080 1 1 1 1 2 1 1005 1205 1105 490 635 563 1 7.1 6.5 6.8
Huntingdon Life Sciences 00-6130 Page 444

211-DIPE-S Final Report

Females

Individual Functional Observational Battery Evaluations

Pretest Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp 

Number Post Vocal Close Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group I - 0 mg/m3

1571 1 1 1 1 2 1 1 1 1 200.3 1 1 1 1 1 0 0 1 1 1

1572 1 1 1 1 2 1 1 1 1 189.9 1 1 1 1 1 0 0 1 1 1

1573 1 1 1 1 1 1 1 1 1 212.0 1 1 1 1 1 0 0 1 1 1

1574 1 1 1 1 1 1 1 1 1 199.2 1 1 1 1 1 0 0 1 1 1

1575 1 1 1 1 1 1 1 1 1 186.6 1 1 1 1 1 0 2 1 1 1

1576 1 1 1 1 2 1 1 1 1 182.1 1 1 1 1 1 0 0 1 1 1

1577 1 1 1 1 2 1 1 1 1 187.0 1 1 1 1 1 0 0 1 1 1

1578 1 1 1 1 2 1 1 1 1 207.6 1 1 1 1 1 0 0 1 1 1

1579 2 1 1 1 2 1 1 1 1 207.5 1 1 1 1 1 1 1 1 1 1

1580 1 1 1 1 2 1 1 1 1 197.9 1 1 1 1 1 0 0 1 1 1

Group II – 2000 mg/m3

2561 2 1 1 1 2 1 1 1 1 191.6 1 1 1 1 1 0 0 1 1 1

2562 1 1 1 1 2 1 1 1 1 202.8 1 1 1 1 1 0 0 1 1 1

2563 1 1 1 1 2 1 1 1 1 195.7 1 1 1 1 1 0 0 1 1 1

2564 1 1 1 1 1 1 1 1 1 198.1 1 1 1 1 1 0 0 1 1 1

2565 1 1 1 1 1 1 1 1 1 182.5 1 1 1 1 1 0 0 1 1 1

2566 1 1 1 1 2 1 1 1 1 182.2 1 1 1 1 1 0 0 1 1 1

2567 1 1 1 1 2 1 1 1 1 185.6 1 1 1 1 1 0 0 1 1 1

2568 1 1 1 1 2 1 1 1 1 202.4 1 1 1 1 1 0 0 1 1 1

2569 1 1 1 1 2 1 1 1 1 201.8 1 1 1 1 1 0 0 1 1 1

2570 2 1 1 1 2 1 1 1 1 203.1 1 1 1 1 1 0 0 1 1 1
Huntingdon Life Sciences 00-6130 Page 445

211-DIPE-S Final Report

Females

Individual Functional Observational Battery Evaluations

Pretest Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group I - 0 mg/m3

1571 1 1 1 1 2 1 1145 1030 1088 830 620 725 1 4.8 5.3 5.1

1572 1 1 1 1 2 1 695 795 745 755 670 713 1 4.8 5.3 5.1

1573 1 1 1 1 2 1 860 775 818 475 765 620 1 3.8 3.6 3.7

1574 1 1 1 1 2 1 900 1045 973 680 770 725 1 5.7 5.1 5.4

1575 1 1 1 1 2 1 900 900 900 620 650 635 1 6.8 5.2 6.0

1576 1 1 1 1 2 1 665 815 740 575 620 598 1 5.3 4.9 5.1

1577 1 1 1 1 2 1 765 910 838 615 550 583 1 4.8 5.3 5.1

1578 1 1 1 1 2 1 910 805 858 615 375 495 1 4.2 3.7 4.0

1579 1 1 1 1 2 1 970 990 980 685 695 690 1 6.8 4.1 5.5

1580 1 1 1 1 2 1 865 950 908 855 805 830 1 5.3 3.9 4.6

Group II – 2000 mg/m3

2561 1 1 1 1 2 1 855 710 783 645 430 538 1 5.6 5.3 5.5

2562 1 1 1 1 2 1 475 870 673 625 635 630 1 4.3 5.7 5.0

2563 1 1 1 1 2 1 1070 880 975 805 610 708 1 6.2 5.3 5.8

2564 1 1 1 1 2 1 695 955 825 775 365 570 1 4.6 3.5 4.1

2565 1 1 1 1 2 1 885 1010 948 480 695 588 1 6.0 6.0 6.0

2566 1 1 1 1 2 1 1150 1145 1148 800 505 653 1 6.0 5.7 5.9

2567 1 1 1 1 2 1 980 1075 1028 610 630 620 1 4.5 4.2 4.4

2568 1 1 1 1 2 1 775 1005 890 480 435 458 1 5.0 4.9 5.0

2569 1 1 1 1 2 1 1040 995 1018 620 455 538 1 6.2 5.1 5.7

2570 1 1 1 1 2 1 850 985 918 735 595 665 1 5.6 4.8 5.2
Huntingdon Life Sciences 00-6130 Page 446

211-DIPE-S Final Report

Females

Individual Functional Observational Battery Evaluations

Pretest Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp 

Number Post Vocal Close Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Tre Conv

(grams)

Group III – 10000 mg/m3

3561 1 1 1 1 2 1 1 1 1 190.8 1 1 1 1 1 0 0 1 1 1

3562 1 1 1 1 2 1 1 1 1 175.2 1 1 1 1 1 0 0 1 1 1

3563 1 1 1 1 1 1 1 1 1 196.0 1 1 1 1 1 0 2 1 1 1

3564 1 1 1 1 1 1 1 1 1 209.8 1 1 1 1 1 0 0 1 1 1

3565 1 1 1 1 1 1 1 1 1 209.4 1 1 1 1 1 0 0 1 1 1

3566 1 1 1 1 2 1 1 1 1 179.8 1 1 1 1 1 0 1 1 1 1

3567 2 1 1 1 2 1 1 1 1 187.2 1 1 1 1 1 0 0 1 1 1

3568 1 1 1 1 2 1 1 1 1 216.6 1 1 1 1 1 0 3 1 1 1

3569 1 1 1 1 2 1 1 1 1 195.7 1 1 1 1 1 0 1 1 1 1

3570 1 1 1 1 2 1 1 1 1 194.6 1 1 1 1 1 0 0 1 1 1

Group IV – 20000 mg/m3

4571 1 1 1 1 2 1 1 1 1 203.8 1 1 1 1 1 1 0 1 1 1

4572 1 1 1 1 2 1 1 1 1 181.2 1 1 1 1 1 0 0 1 1 1

4573 1 1 1 1 2 1 1 1 1 188.3 1 1 1 1 1 0 1 1 1 1

4574 1 1 1 1 1 1 1 1 1 197.9 1 1 1 1 1 0 0 1 1 1

4575 1 1 1 1 1 1 1 1 1 188.1 1 1 1 1 1 0 0 1 1 1

4576 1 1 1 1 2 1 1 1 1 190.9 1 1 1 1 1 0 1 1 1 1

4577 1 1 1 1 2 1 1 1 1 187.2 1 1 1 1 1 0 0 1 1 1

4578 1 1 1 1 2 1 1 1 1 188.7 1 1 1 1 1 0 0 1 1 1

4579 1 1 1 1 2 1 1 1 1 209.5 1 1 1 1 1 0 0 1 1 1

4580 1 1 1 1 2 1 1 1 1 215.2 1 1 1 1 1 0 0 1 1 1
Huntingdon Life Sciences 00-6130 Page 447

211-DIPE-S Final Report

Females

Individual Functional Observational Battery Evaluations

Pretest Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean 

Group III – 10000 mg/m3

3561 1 1 1 1 2 1 835 945 890 705 510 608 1 7.3 5.8 6.6

3562 1 1 1 1 2 1 945 865 905 690 615 653 1 5.2 4.0 4.6

3563 1 1 1 1 2 1 720 885 803 550 625 588 1 6.2 5.3 5.8

3564 1 1 1 1 2 1 985 1120 1053 500 420 460 1 5.1 5.0 5.1

3565 1 1 1 1 2 1 705 795 750 405 395 400 1 3.1 2.8 3.0

3566 1 1 1 1 2 1 1015 785 900 575 445 510 1 6.2 5.3 5.8

3567 1 1 1 1 2 1 965 780 873 580 575 578 1 5.3 5.5 5.4

3568 1 1 1 1 2 1 935 835 885 655 485 570 1 6.8 6.6 6.7

3569 1 1 1 1 2 1 885 1000 943 870 645 758 1 4.0 3.8 3.9

3570 1 1 1 1 2 1 1000 960 980 660 755 708 1 4.6 4.9 4.8

Group IV – 20000 mg/m3

4571 1 1 1 1 2 1 850 970 910 610 420 515 1 6.1 5.9 6.0

4572 1 1 1 1 2 1 670 605 638 495 575 535 1 5.2 6.0 5.6

4573 1 1 1 1 2 1 950 1035 993 645 605 625 1 4.3 3.1 3.7

4574 1 1 1 1 2 1 945 1015 980 775 645 710 1 4.6 4.2 4.4

4575 1 1 1 1 2 1 1050 945 998 670 610 640 1 5.2 4.3 4.8

4576 1 1 1 1 2 1 1085 965 1025 535 705 620 1 6.2 6.8 6.5

4577 1 1 1 1 2 1 705 975 840 585 625 605 1 7.0 7.9 7.5

4578 1 1 1 1 1 1 570 845 708 690 605 648 1 5.8 5.3 5.6

4579 1 1 1 1 2 1 745 1075 910 620 465 543 1 4.1 4.0 4.1

4580 1 1 1 1 2 1 815 1075 945 705 920 813 1 5.6 5.2 5.4
Huntingdon Life Sciences 00-6130 Page 448

211-DIPE-S Final Report

Males

Individual Functional Observational Battery Evaluations

4th week of exposure Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp 

Number Post Vocal Close Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group I - 0 mg/m3

1071 1 1 1 1 2 1 1 1 1 448.3 1 1 1 1 1 0 0 1 1 1

1072 1 1 1 1 2 1 1 1 1 366.2 1 1 1 1 1 7 3 1 1 1

1073 1 1 1 1 2 1 1 1 1 397.1 1 1 1 1 1 0 0 1 1 1

1074 1 1 1 1 2 1 1 1 1 340.6 1 1 1 1 1 2 0 1 1 1

1075 1 1 1 1 2 1 1 1 1 372.8 1 1 1 1 1 0 0 1 1 1

1076 1 1 1 1 2 1 1 1 1 445.5 1 1 1 1 1 0 5 1 1 1

1077 1 1 1 1 2 1 1 1 1 407.0 1 1 1 1 1 0 0 1 1 1

1078 1 1 1 1 2 1 1 1 1 387.5 1 1 1 1 1 1 4 1 1 1

1079 1 1 1 1 2 1 1 1 1 405.9 1 1 1 1 1 0 0 1 1 1

1080 2 1 1 1 2 1 1 1 1 400.3 1 1 1 1 1 0 0 1 1 1

Group II – 2000 mg/m3

2061 1 1 1 1 2 1 1 1 1 360.9 1 1 1 1 1 0 0 1 1 1

2062 1 1 1 1 2 1 1 1 1 384.0 1 1 1 1 1 0 0 1 1 1

2063 1 1 1 1 2 1 1 1 1 407.1 1 1 1 1 1 0 0 1 1 1

2064 1 1 1 1 2 1 1 1 1 345.6 1 1 1 1 1 5 5 1 1 1

2065 1 1 1 1 2 1 1 1 1 448.2 1 1 1 1 1 0 3 1 1 1

2066 1 1 1 1 2 1 1 1 1 419.2 1 1 1 1 1 0 5 1 1 1

2067 1 1 1 1 2 1 1 1 1 393.6 1 1 1 1 1 0 0 1 1 1

2068 2 1 1 1 2 1 1 1 1 434.5 1 1 1 1 1 0 2 1 1 1

2069 1 1 1 1 2 1 1 1 1 425.0 1 1 1 1 1 0 0 1 1 1

2070 1 1 1 1 2 1 1 1 1 344.4 1 1 1 1 1 0 3 1 1 1
Huntingdon Life Sciences 00-6130 Page 449

211-DIPE-S Final Report

Males

Individual Functional Observational Battery Evaluations

4th week of exposure Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group I - 0 mg/m3

1071 1 1 1 1 2 1 1405 1360 1383 765 705 735 1 7.7 6.8 7.3

1072 1 1 1 1 2 1 1380 1235 1308 755 960 858 1 6.0 5.2 5.6

1073 1 1 1 1 2 1 1495 1250 1373 785 1125 955 1 6.5 7.2 6.9

1074 1 1 1 1 2 1 1230 1585 1408 875 925 900 1 8.8 7.2 8.0

1075 1 1 1 1 2 1 1305 1225 1265 640 705 673 1 11.2 9.6 10.4

1076 1 1 1 1 2 1 1050 1335 1193 770 805 788 1 5.7 4.8 5.3

1077 1 1 1 1 2 1 1335 1180 1258 1030 835 933 1 9.8 8.7 9.3

1078 2 1 1 1 2 1 1345 1440 1393 925 1150 1038 1 7.3 5.6 6.5

1079 1 1 1 1 2 1 1110 1230 1170 835 810 823 1 7.6 6.3 7.0

1080 1 1 1 1 2 1 1010 900 955 740 550 645 1 5.4 5.1 5.3

Group II – 2000 mg/m3

2061 1 1 1 1 2 1 1480 1095 1288 580 635 608 1 7.0 7.3 7.2

2062 1 1 1 1 2 1 1440 1090 1265 825 785 805 1 7.6 7.7 7.7

2063 1 1 1 1 2 1 1505 1540 1523 1010 1280 1145 1 6.1 5.8 6.0

2064 1 1 1 1 2 1 1240 1230 1235 455 475 465 1 7.7 6.8 7.3

2065 1 1 1 1 1 1 1035 1235 1135 915 660 788 1 5.4 5.3 5.4

2066 1 1 1 1 2 1 1235 1265 1250 830 750 790 1 5.6 5.6 5.6

2067 1 1 1 1 2 1 1270 1520 1395 770 560 665 1 8.0 7.0 7.5

2068 1 1 1 1 2 1 1320 1670 1495 820 805 813 1 5.6 6.5 6.1

2069 1 1 1 1 2 1 1070 1315 1193 805 835 820 1 5.3 5.1 5.2

2070 1 1 1 1 2 1 1455 1535 1495 890 930 910 1 6.2 7.5 6.9
Huntingdon Life Sciences 00-6130 Page 450

211-DIPE-S Final Report

Males

Individual Functional Observational Battery Evaluations

4th week of exposure Appendix H

a

Opens and closes mouth repeatedly.

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp 

Number Post Vocal Close Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group III – 10000 mg/m3

3061 1 1 1 2 2 1 1 1 1 418.2 1 1 1 1 1 3 0 1 1 1

3062 1 1 1 1 2 1 1 1 1 414.6 1 1 1 1 1 0 6 1 1 1

3063 1 1 1 1 2 1 1 1 1 395.0 1 1 1 1 1 5 1 1 1 1

3064 1 1 1 1 2 1 1 1 1 420.5 1 1 1 1 1 4 1 1 1 1

3065 2 1 1 1 2 1 1 1 1 345.9 1 1 1 1 1 0 0 1 1 1

3066 1 1 1 1 2 1 1 1 1 377.4 1 1 1 1 1 3 1 1 1 1

3067 1 1 1 2 2 1 1 1 1 451.6 1 1 1 1 1 5 0 1 1 1

3068 2 1 1 2 2 1 1 1 1 423.5 1 1 1 1 1 0 0 1 1 1

3069 1 1 1 1 2 1 1 1 1 431.7 1 1 1 1 1 0 0 1 1 1

3070 1 1 1 1 2 1 1 1 1 422.1 1 1 1 1 1 5 0 1 1 1

Group IV – 20000 mg/m3

4071 1 1 1 1 2 1 1 1 1 379.8 1 1 1 1 1 1 0 1 1 1

4072 1 1 1 1 2 1 1 1 1 430.1 1 1 1 1 1 6 0 1 1 1

4073 1 1 1 1 2 1 1 1 1 418.6 1 1 1 1 1 4 2 1 1 1

4074 1 1 1 1 2 1 1 1 1 400.5 1 1 1 1 1 2 2 1 1 1

4075 1 1 1 1 2 1 1 1 1 368.4 1 1 1 1 1 0 2 1 1 1

4076 1 1 1 1 2 1 1 1 1 429.1 1 1 1 1 1 0 0 1 1 1

4077 1 1 1 1 2 1 1 1 1 379.1 1 1 1 1 1 0 0 1 1 1

4078a 2 1 1 1 2 1 1 1 1 424.4 1 1 1 1 1 0 0 1 1 1

4079 1 1 1 1 2 1 1 1 1 424.2 1 1 1 1 1 0 2 1 1 1

4080 1 1 1 1 2 1 1 1 1 401.2 1 1 1 1 1 1 0 1 1 1
Huntingdon Life Sciences 00-6130 Page 451

211-DIPE-S Final Report

Males

Individual Functional Observational Battery Evaluations

4th week of exposure Appendix H

a

Opens and closes mouth repeatedly.

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Numbe Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group III – 10000 mg/m3

3061 1 1 1 1 2 1 1255 1315 1285 830 560 695 1 7.0 5.3 6.2

3062 1 1 1 1 2 1 810 850 830 1050 920 985 1 4.6 4.2 4.4

3063 1 1 1 1 2 1 1400 1470 1435 720 790 755 1 7.1 7.3 7.2

3064 1 1 1 1 2 1 1375 1400 1388 500 420 460 1 7.7 7.2 7.5

3065 1 1 1 1 2 1 1510 1710 1610 535 720 628 1 5.8 4.3 5.1

3066 1 1 1 1 2 1 1410 1585 1498 1130 795 963 1 8.1 7.7 7.9

3067 1 1 1 1 2 1 1490 1375 1433 1470 1280 1375 1 4.8 4.9 4.9

3068 1 1 1 1 2 1 1355 1475 1415 610 810 710 1 5.6 5.6 5.6

3069 1 1 1 1 2 1 1420 990 1205 680 820 750 1 8.3 8.1 8.2

3070 1 1 1 1 2 1 1220 1145 1183 1070 1265 1168 1 8.1 6.3 7.2

Group IV – 20000 mg/m3

4071 1 1 1 1 2 1 1325 1355 1340 595 685 640 1 7.2 6.8 7.0

4072 1 1 1 1 2 1 1265 1200 1233 1155 1175 1165 1 10.3 9.7 10.0

4073 1 1 1 1 2 1 1275 1155 1215 620 550 585 1 6.5 5.9 6.2

4074 1 1 1 1 2 1 1295 1310 1303 1165 1020 1093 1 5.8 5.9 5.9

4075 1 1 1 1 2 1 1135 1410 1273 700 845 773 1 5.7 3.7 4.7

4076 1 1 1 1 2 1 1335 890 1113 805 755 780 1 5.8 5.7 5.8

4077 1 1 1 1 2 1 1475 1255 1365 1195 840 1018 1 7.3 7.1 7.2

4078a 1 1 1 1 2 1 1490 1665 1578 1010 1030 1020 1 5.2 6.3 5.8

4079 1 1 1 1 2 1 1440 1585 1513 1130 965 1048 1 10.3 10.2 10.3

4080 1 1 1 1 2 1 1285 1405 1345 870 565 718 1 7.2 6.6 6.9
Huntingdon Life Sciences 00-6130 Page 452

211-DIPE-S Final Report

Females

Individual Functional Observational Battery Evaluations

4th week of exposure Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp 

Number Post Vocal Close Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams

Group I - 0 mg/m3

1571 1 1 1 1 2 1 1 1 1 274.3 1 1 1 1 1 0 0 1 1 1

1572 1 1 1 1 2 1 1 1 1 265.4 1 1 1 1 1 0 0 1 1 1

1573 1 1 1 1 2 1 1 1 1 297.9 1 1 1 1 1 0 0 1 1 1

1574 1 1 1 1 2 1 1 1 1 276.7 1 1 1 1 1 0 0 1 1 1

1575 3 1 2 1 2 1 1 1 1 237.8 1 1 1 1 1 4 2 1 1 1

1576 1 1 1 1 2 1 1 1 1 264.2 1 1 1 1 1 0 0 1 1 1

1577 1 1 1 1 2 1 1 1 1 252.6 1 1 1 1 1 0 0 1 1 1

1578 2 1 1 1 2 1 1 1 1 279.5 1 1 1 1 1 0 0 1 1 1

1579 1 1 1 1 2 1 1 1 1 302.3 1 1 1 1 1 0 0 1 1 1

1580 2 1 1 1 2 1 1 1 1 264.3 1 1 1 1 1 0 0 1 1 1

Group II – 2000 mg/m3

2561 2 1 1 1 2 1 1 1 1 244.0 1 1 1 1 1 0 0 1 1 1

2562 1 1 1 1 2 1 1 1 1 293.5 1 1 1 1 1 0 0 1 1 1

2563 1 1 1 1 1 1 1 1 1 260.1 1 1 1 1 1 0 0 1 1 1

2564 2 1 1 1 2 1 1 1 1 260.1 1 1 1 1 1 0 0 1 1 1

2565 2 1 1 1 2 1 1 1 1 264.1 1 1 1 1 1 0 0 1 1 1

2566 1 1 1 1 2 1 1 1 1 215.9 1 1 1 1 1 0 0 1 1 1

2567 1 1 1 1 2 1 1 1 1 250.2 1 1 1 1 1 0 0 1 1 1

2568 3 1 2 1 2 1 1 1 1 291.3 1 1 1 1 1 0 0 1 1 1

2569 1 1 1 1 2 1 1 1 1 266.8 1 1 1 1 1 0 0 1 1 1

2570 1 1 1 1 2 1 1 1 1 277.0 1 1 1 1 1 0 0 1 1 1
Huntingdon Life Sciences 00-6130 Page 453

211-DIPE-S Final Report

Females

Individual Functional Observational Battery Evaluations

4th week of exposure Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group I - 0 mg/m3

1571 1 1 1 1 2 1 1260 1140 1200 525 685 605 1 5.2 6.3 5.8

1572 1 1 1 1 2 1 1105 1020 1063 830 810 820 1 4.2 3.6 3.9

1573 1 1 1 1 1 1 1240 975 1108 760 730 745 1 3.9 4.3 4.1

1574 1 1 1 1 2 1 1145 1440 1293 765 710 738 1 5.7 5.1 5.4

1575 1 1 1 1 2 1 1105 1050 1078 805 505 655 1 6.0 5.4 5.7

1576 1 1 1 1 2 1 1000 1345 1173 855 1130 993 1 6.6 5.2 5.9

1577 1 1 1 1 2 1 1125 1285 1205 795 875 835 1 6.0 4.9 5.5

1578 1 1 1 1 2 1 1115 1340 1228 660 655 658 1 6.1 6.7 6.4

1579 1 1 1 1 2 1 1245 1180 1213 930 855 893 1 6.2 5.0 5.6

1580 1 1 1 1 2 1 1365 1100 1233 955 910 933 1 6.6 5.9 6.3

Group II – 2000 mg/m3

2561 1 1 1 1 2 1 1225 1205 1215 720 840 780 1 4.3 4.8 4.6

2562 1 1 1 1 2 1 1130 970 1050 855 940 898 1 5.4 5.3 5.4

2563 1 1 1 1 2 1 1570 1050 1310 825 855 840 1 6.2 5.8 6.0

2564 1 1 1 1 2 1 1285 1175 1230 895 955 925 1 3.5 4.8 4.2

2565 1 1 1 1 2 1 1235 1055 1145 695 665 680 1 6.1 5.7 5.9

2566 1 1 1 1 2 1 1305 1305 1305 530 625 578 1 5.0 4.6 4.8

2567 1 1 1 1 2 1 1290 1180 1235 785 805 795 1 6.2 7.0 6.6

2568 1 1 1 1 2 1 915 1040 978 455 525 490 1 5.2 4.5 4.9

2569 1 1 1 1 2 1 1145 1065 1105 800 835 818 1 4.8 4.4 4.6

2570 1 1 1 1 2 1 1040 1245 1143 900 835 868 1 5.1 5.8 5.5
Huntingdon Life Sciences 00-6130 Page 454

211-DIPE-S Final Report

Females

Individual Functional Observational Battery Evaluations

4th week of exposure Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp 

Number Post Vocal Close Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group III – 10000 mg/m3

3561 1 1 1 1 2 1 1 1 1 248.4 1 1 1 1 1 0 0 1 1 1

3562 1 1 1 1 2 1 1 1 1 219.7 1 1 1 1 1 0 1 1 1 1

3563 2 1 1 1 2 1 1 1 1 236.5 1 1 1 1 1 1 1 1 1 1

3564 2 1 1 1 2 1 1 1 1 280.2 1 1 1 1 1 0 0 1 1 1

3565 1 1 1 1 2 1 1 1 1 306.6 1 1 1 1 1 0 0 1 1 1

3566 2 1 1 1 2 1 1 1 1 240.7 1 1 1 1 1 0 0 1 1 1

3567 2 1 1 1 2 1 1 1 1 237.7 1 1 1 1 1 0 0 1 1 1

3568 1 1 1 1 1 1 1 1 1 279.1 1 1 1 1 1 0 1 1 1 1

3569 1 1 1 1 1 1 1 1 1 240.8 1 1 1 1 1 0 0 1 1 1

3570 1 1 1 1 2 1 1 1 1 254.4 1 1 1 1 1 0 0 1 1 1

Group IV – 20000 mg/m3

4571 1 1 1 1 2 1 1 1 1 276.2 1 1 1 1 1 0 0 1 1 1

4572 1 1 1 1 2 1 1 1 1 258.9 1 1 1 1 1 0 0 1 1 1

4573 1 1 1 1 2 1 1 1 1 250.6 1 1 1 1 1 0 0 1 1 1

4574 1 1 1 1 2 1 1 1 1 240.6 1 1 1 1 1 0 0 1 1 1

4575 2 1 1 1 2 1 1 1 1 250.8 1 1 1 1 1 0 0 1 1 1

4576 2 1 1 2 2 1 1 1 1 237.9 1 1 1 1 1 0 0 1 1 1

4577 2 1 1 1 2 1 1 1 1 237.1 1 1 1 1 1 0 0 1 1 1

4578 1 1 1 1 2 1 1 1 1 250.5 1 1 1 1 1 0 0 1 1 1

4579 2 1 1 1 2 1 1 1 1 261.3 1 1 1 1 1 0 0 1 1 1

4580 2 1 1 1 2 1 1 1 1 268.6 1 1 1 1 1 0 0 1 1 1
Huntingdon Life Sciences 00-6130 Page 455

211-DIPE-S Final Report

Females

Individual Functional Observational Battery Evaluations

4th week of exposure Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group III – 10000 mg/m3

3561 1 1 1 1 2 1 1295 1425 1360 890 975 933 1 7.2 6.8 7.0

3562 1 1 1 1 2 1 750 1000 875 785 660 723 1 6.2 4.1 5.2

3563 1 1 1 1 1 1 1250 1170 1210 700 755 728 1 5.2 4.7 5.0

3564 1 1 1 1 2 1 1405 1145 1275 820 515 668 1 4.6 5.6 5.1

3565 1 1 1 1 2 1 1150 1385 1268 680 505 593 1 5.6 4.7 5.2

3566 1 1 1 1 2 1 845 885 865 830 830 830 1 4.6 4.1 4.4

3567 1 1 1 1 2 1 1120 1210 1165 875 750 813 1 5.2 4.4 4.8

3568 1 1 1 1 2 1 1100 865 983 950 695 823 1 8.8 6.9 7.9

3569 1 1 1 1 2 1 1400 1320 1360 985 810 898 1 6.3 6.0 6.2

3570 1 1 1 1 2 1 1315 1420 1368 825 840 833 1 4.3 4.2 4.3

Group IV – 20000 mg/m3

4571 1 1 1 1 2 1 1310 825 1068 580 485 533 1 7.8 8.6 8.2

4572 1 1 1 1 2 1 1335 1000 1168 520 755 638 1 6.6 6.5 6.6

4573 1 1 1 1 2 1 1040 1160 1100 610 610 610 1 4.7 4.0 4.4

4574 1 1 1 1 2 1 1215 1355 1285 675 730 703 1 5.2 4.6 4.9

4575 1 1 1 1 2 1 1220 1530 1375 815 870 843 1 6.2 5.1 5.7

4576 1 1 1 1 2 1 1495 1085 1290 1050 630 840 1 6.5 6.8 6.7

4577 1 1 1 1 2 1 1015 1285 1150 585 660 623 1 7.8 7.2 7.5

4578 1 1 1 1 2 1 1050 1035 1043 805 795 800 1 5.2 5.1 5.2

4579 1 1 1 1 2 1 1095 1205 1150 880 510 695 1 6.3 5.6 6.0

4580 1 1 1 1 2 1 1280 1145 1213 980 695 838 1 5.6 5.3 5.5
Huntingdon Life Sciences 00-6130 Page 456

211-DIPE-S Final Report

Males

Individual Functional Observational Battery Evaluations

8th week of exposure Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp 

Number Post Vocal Close Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group I - 0 mg/m3

1071 1 1 1 1 2 1 1 1 1 569.1 1 1 1 1 1 1 0 1 1 1

1072 1 1 1 1 2 1 1 1 1 471.6 1 1 1 1 1 0 0 1 1 1

1073 2 1 1 2 2 1 1 1 1 479.4 1 1 1 1 1 0 0 1 1 1

1074 1 1 1 1 2 1 1 1 1 411.0 1 1 1 1 1 5 1 1 1 1

1075 1 1 1 2 2 1 1 1 1 454.2 1 1 1 1 1 3 0 1 1 1

1076 1 1 1 1 2 1 1 1 1 555.1 1 1 1 1 1 0 7 1 1 1

1077 2 1 1 1 2 1 1 1 1 482.3 1 1 1 1 1 0 0 1 1 1

1078 1 1 1 1 2 1 1 1 1 456.1 1 1 1 1 1 0 0 1 1 1

1079 1 1 1 2 2 1 1 1 1 476.6 1 1 1 1 1 0 0 1 1 1

1080 1 1 1 1 2 1 1 1 1 495.2 1 1 1 1 1 0 0 1 1 1

Group II – 2000 mg/m3

2061 1 1 1 1 2 1 1 1 1 432.8 1 1 1 1 1 2 0 1 1 1

2062 1 1 1 1 2 1 1 1 1 483.5 1 1 1 1 1 1 0 1 1 1

2063 1 1 1 1 2 1 1 1 1 514.2 1 1 1 1 1 0 0 1 1 1

2064 2 1 1 2 2 1 1 1 1 403.9 1 1 1 1 1 5 3 1 1 1

2065 2 1 1 1 2 1 1 1 1 546.9 1 1 1 1 1 0 3 1 1 1

2066 1 1 1 1 2 1 1 1 1 507.0 1 1 1 1 1 3 3 1 1 1

2067 1 1 1 1 2 1 1 1 1 470.1 1 1 1 1 1 0 0 1 1 1

2068 1 1 1 1 2 1 1 1 1 517.3 1 1 1 1 1 0 0 1 1 1

2069 1 1 1 1 2 1 1 1 1 502.7 1 1 1 1 1 0 0 1 1 1

2070 1 1 1 2 2 2 2 1 1 252.6 5 3 2 1 1 0 0 1 1 1
Huntingdon Life Sciences 00-6130 Page 457

211-DIPE-S Final Report

Males

Individual Functional Observational Battery Evaluations

8th week of exposure Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group I - 0 mg/m3

1071 1 1 1 1 2 1 1445 1435 1440 780 825 803 1 7.5 8.2 7.9

1072 1 1 1 1 2 1 1245 1365 1305 870 850 860 1 5.1 5.1 5.1

1073 1 1 1 1 2 1 1310 850 1080 855 1840 1348 1 8.9 9.8 9.4

1074 1 1 1 1 2 1 1100 1120 1110 820 935 878 1 7.5 8.0 7.8

1075 1 1 1 1 2 1 1025 975 1000 840 615 728 1 9.6 6.3 8.0

1076 1 1 1 1 2 1 1415 1025 1220 925 735 830 1 6.1 5.2 5.7

1077 1 1 1 1 2 1 1535 1005 1270 825 995 910 1 6.7 6.6 6.7

1078 1 1 1 1 2 1 1555 1400 1478 1105 1295 1200 1 4.9 4.7 4.8

1079 1 1 1 1 2 1 590 1300 945 830 870 850 1 9.6 9.7 9.7

1080 1 1 1 1 2 1 1260 1190 1225 960 660 810 1 6.7 5.5 6.1

Group II – 2000 mg/m3

2061 1 1 1 1 2 1 1245 1505 1375 890 900 895 1 7.4 8.6 8.0

2062 1 1 1 1 2 1 985 1050 1018 900 990 945 1 4.8 5.6 5.2

2063 1 1 1 1 2 1 1405 1455 1430 910 780 845 1 6.9 7.9 7.4

2064 1 1 1 1 2 1 1350 1180 1265 600 585 593 1 6.9 6.7 6.8

2065 1 1 1 1 2 1 1390 1295 1343 705 800 753 1 6.0 5.2 5.6

2066 1 1 1 1 2 1 1070 1080 1075 730 715 723 1 7.3 5.7 6.5

2067 1 1 1 1 2 1 655 1010 833 795 550 673 1 7.7 6.7 7.2

2068 1 1 1 1 2 1 1595 1340 1468 1355 965 1160 1 5.0 4.7 4.9

2069 1 1 1 1 2 1 1130 990 1060 635 580 608 1 5.6 5.7 5.7

2070 1 1 1 1 2 1 950 820 885 870 820 845 1 5.9 6.2 6.1
Huntingdon Life Sciences 00-6130 Page 458

211-DIPE-S Final Report

Males

Individual Functional Observational Battery Evaluations

8th week of exposure Appendix H

a

Opens and closes mouth repeatedly.

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp 

Numbe Post Vocal Close Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group III – 10000 mg/m3

3061 1 1 1 1 2 1 1 1 1 519.2 1 1 1 1 1 3 0 1 1 1

3062 1 1 1 1 2 1 1 1 1 512.8 1 1 1 1 1 0 6 1 1 1

3063 1 1 1 1 2 1 1 1 1 492.6 1 1 1 1 1 0 3 1 1 1

3064 1 1 1 1 2 1 1 1 1 523.4 1 1 1 1 1 5 2 1 1 1

3065 1 1 1 1 2 1 1 1 1 429.3 1 1 1 1 1 1 0 1 1 1

3066 2 1 1 1 2 1 1 1 1 464.7 1 1 1 1 1 0 2 1 1 1

3067 1 1 1 1 2 1 1 1 1 546.4 1 1 1 1 1 0 0 1 1 1

3068 2 1 1 1 2 1 1 1 1 538.7 1 1 1 1 1 0 0 1 1 1

3069 1 1 1 1 2 1 1 1 1 504.3 1 1 1 1 1 0 0 1 1 1

3070 1 1 1 1 2 1 1 1 1 517.4 1 1 1 1 1 5 0 1 1 1

Group IV – 20000 mg/m3

4071 1 1 1 1 2 1 1 1 1 594.4 1 1 1 1 1 0 0 1 1 1

4072 1 1 1 1 2 1 1 1 1 536.3 1 1 1 1 1 0 0 1 1 1

4073 2 1 1 1 2 1 1 1 1 504.2 1 1 1 1 1 5 2 1 1 1

4074 1 1 1 1 2 1 1 1 1 466.6 1 1 1 1 1 4 0 1 1 1

4075 1 1 1 1 2 1 1 1 1 442.9 1 1 1 1 1 1 1 1 1 1

4076 2 1 1 1 2 1 1 1 1 521.8 1 1 1 1 1 0 0 1 1 1

4077 2 1 1 1 2 1 1 1 1 452.8 1 1 1 1 1 0 2 1 1 1

4078a 1 1 1 2 2 1 1 1 1 493.5 1 1 1 1 1 0 0 1 1 1

4079 2 1 1 2 2 1 1 1 1 518.1 1 1 1 1 1 0 0 1 1 1

4080 2 1 1 1 2 1 1 1 1 487.5 1 1 1 1 1 2 1 1 1 1
Huntingdon Life Sciences 00-6130 Page 459

211-DIPE-S Final Report

Males

Individual Functional Observational Battery Evaluations

8th week of exposure Appendix H

a

Opens and closes mouth repeatedly.

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group III – 10000 mg/m3

3061 1 1 1 1 2 1 1280 1295 1288 515 545 530 1 5.1 4.7 4.9

3062 1 1 1 1 2 1 1075 745 910 680 840 760 1 3.5 3.7 3.6

3063 1 1 1 1 2 1 1150 1310 1230 575 595 585 1 5.9 6.2 6.1

3064 1 1 1 1 2 1 1310 1210 1260 475 540 508 1 9.1 7.7 8.4

3065 1 1 1 1 2 1 1350 1375 1363 785 830 808 1 5.5 4.4 5.0

3066 1 1 1 1 2 1 1390 1370 1380 950 655 803 1 7.1 6.9 7.0

3067 1 1 1 1 2 1 1280 1245 1263 1070 1040 1055 1 7.4 5.7 6.6

3068 1 1 1 1 2 1 960 880 920 605 1015 810 1 5.3 5.1 5.2

3069 1 1 1 1 2 1 1295 850 1073 860 745 803 1 8.3 6.6 7.5

3070 1 1 1 1 2 1 930 460 695 1655 1795 1725 1 5.4 4.5 5.0

Group IV – 20000 mg/m3

4071 1 1 1 1 2 1 1520 1565 1543 630 660 645 1 7.2 7.1 7.2

4072 1 1 1 1 2 1 1535 1295 1415 1130 1045 1088 1 7.4 6.8 7.1

4073 1 1 1 1 2 1 1260 1125 1193 590 640 615 1 7.3 6.1 6.7

4074 1 1 1 1 2 1 610 1130 870 880 660 770 1 6.8 6.0 6.4

4075 1 1 1 1 2 1 1195 1270 1233 775 700 738 1 5.3 5.5 5.4

4076 1 1 1 1 2 1 975 1370 1173 885 805 845 1 6.0 5.9 6.0

4077 1 1 1 1 2 1 1305 1275 1290 860 865 863 1 5.6 5.4 5.5

 4078a 1 1 1 1 2 1 1420 1465 1443 835 1050 943 1 5.4 5.0 5.2

4079 1 1 1 1 2 1 1315 1430 1373 930 1040 985 1 6.0 6.3 6.2

4080 1 1 1 1 2 1 1360 900 1130 1025 1175 1100 1 6.7 7.9 7.3
Huntingdon Life Sciences 00-6130 Page 460

211-DIPE-S Final Report

Females

Individual Functional Observational Battery Evaluations

8th week of exposure Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp 

Numbe Post Vocal Close Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams

Group I - 0 mg/m3

1571 2 1 1 1 2 1 1 1 1 309.3 1 1 1 1 1 0 0 1 1 1

1572 1 1 1 1 2 1 1 1 1 296.6 1 1 1 1 1 0 0 1 1 1

1573 2 1 1 1 2 1 1 1 1 339.7 1 1 1 1 1 0 0 1 1 1

1574 1 1 1 1 2 1 1 1 1 321.6 1 1 1 1 1 0 0 1 1 1

1575 3 1 2 1 2 1 1 1 1 253.4 1 1 1 1 1 0 3 1 1 1

1576 1 1 1 1 2 1 1 1 1 285.6 1 1 1 1 1 0 0 1 1 1

1577 2 1 1 1 2 1 1 1 1 275.9 1 1 1 1 1 0 0 1 1 1

1578 2 1 1 1 2 1 1 1 1 304.3 1 1 1 1 1 0 0 1 1 1

1579 1 1 1 1 2 1 1 1 1 350.4 1 1 1 1 1 0 0 1 1 1

1580 2 1 1 1 2 1 1 1 1 288.4 1 1 1 1 1 0 0 1 1 1

Group II – 2000 mg/m3

2561 2 1 1 1 2 1 1 1 1 265.1 1 1 1 1 1 0 0 1 1 1

2562 2 1 1 1 2 1 1 1 1 323.7 1 1 1 1 1 0 0 1 1 1

2563 2 1 1 1 2 1 1 1 1 289.6 1 1 1 1 1 0 0 1 1 1

2564 2 1 1 1 2 1 1 1 1 291.3 1 1 1 1 1 0 0 1 1 1

2565 1 1 1 1 2 1 1 1 1 293.7 1 1 1 1 1 0 0 1 1 1

2566 1 1 1 1 2 1 1 1 1 229.8 1 1 1 1 1 0 0 1 1 1

2567 1 1 1 1 2 1 1 1 1 285.6 1 1 1 1 1 2 2 1 1 1

2568 2 1 1 1 2 1 1 1 1 316.5 1 1 1 1 1 0 0 1 1 1

2569 1 1 1 1 2 1 1 1 1 288.4 1 1 1 1 1 0 0 1 1 1

2570 1 1 1 1 2 1 1 1 1 304.1 1 1 1 1 1 0 0 1 1 1
Huntingdon Life Sciences 00-6130 Page 461

211-DIPE-S Final Report

Females

Individual Functional Observational Battery Evaluations

8th week of exposure Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group I - 0 mg/m3

1571 1 1 1 1 2 1 1380 1050 1215 690 900 795 1 5.6 4.7 5.2

1572 1 1 1 1 2 1 1135 1050 1093 715 895 805 1 3.9 4.5 4.2

1573 1 1 1 1 2 1 1185 1185 1185 575 590 583 1 4.6 4.5 4.6

1574 1 1 1 1 2 1 1125 955 1040 870 900 885 1 5.8 5.6 5.7

1575 1 1 1 1 2 1 930 845 888 585 550 568 1 5.5 5.2 5.4

1576 1 1 1 1 2 1 1405 1180 1293 785 775 780 1 5.5 4.7 5.1

1577 1 1 1 1 2 1 1245 1330 1288 560 570 565 1 4.9 4.4 4.7

1578 1 1 1 1 2 1 1080 1490 1285 880 825 853 1 4.7 4.8 4.8

1579 1 1 1 1 2 1 905 1445 1175 750 885 818 1 5.7 4.1 4.9

1580 1 1 1 1 2 1 1410 1055 1233 1020 1175 1098 1 6.1 4.4 5.3

Group II – 2000 mg/m3

2561 1 1 1 1 2 1 1145 1055 1100 400 760 580 1 4.1 4.2 4.2

2562 1 1 1 1 2 1 880 895 888 985 950 968 1 4.8 3.7 4.3

2563 1 1 1 1 2 1 1070 660 865 815 805 810 1 5.7 5.2 5.5

2564 1 1 1 1 2 1 995 1480 1238 830 1040 935 1 4.2 3.9 4.1

2565 1 1 1 1 2 1 970 985 978 840 635 738 1 6.4 6.3 6.4

2566 1 1 1 1 1 1 1100 1045 1073 530 500 515 1 5.1 4.1 4.6

2567 1 1 1 1 2 1 1300 1050 1175 880 985 933 1 5.1 6.9 6.0

2568 1 1 1 1 2 1 1195 495 845 830 720 775 1 5.1 5.0 5.1

2569 1 1 1 1 2 1 865 950 908 640 835 738 1 4.8 5.2 5.0

2570 1 1 1 1 2 1 1145 1315 1230 675 845 760 1 5.5 4.8 5.2
Huntingdon Life Sciences 00-6130 Page 462

211-DIPE-S Final Report

Females

Individual Functional Observational Battery Evaluations

8th week of exposure Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp 

Number Post Vocal Close Rem Hand Chromo Lac Sa Coat BW Gai Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group III – 10000 mg/m3

3561 2 1 1 1 2 1 1 1 1 279.1 1 1 1 1 1 0 0 1 1 1

3562 1 1 1 1 2 1 1 1 1 234.5 1 1 1 1 1 0 0 1 1 1

3563 1 1 1 1 2 1 1 1 1 254.4 1 1 1 1 1 0 0 1 1 1

3564 1 1 1 1 2 1 1 1 1 309.1 1 1 1 1 1 0 0 1 1 1

3565 2 1 1 1 2 1 1 1 1 351.7 1 1 1 1 1 0 0 1 1 1

3566 2 1 1 1 2 1 1 1 1 273.1 1 1 1 1 1 0 0 1 1 1

3567 2 1 1 1 2 1 1 1 1 258.5 1 1 1 1 1 0 0 1 1 1

3568 1 1 1 1 2 1 1 1 1 305.6 1 1 1 1 1 0 0 1 1 1

3569 1 1 1 1 2 1 1 1 1 262.3 1 1 1 1 1 0 0 1 1 1

3570 1 1 1 1 2 1 1 1 1 274.6 1 1 1 1 1 0 0 1 1 1

Group IV – 20000 mg/m3

4571 1 1 1 1 2 1 1 1 1 324.3 1 1 1 1 1 0 0 1 1 1

4572 1 1 1 1 2 1 1 1 1 282.2 1 1 1 1 1 0 0 1 1 1

4573 1 1 1 1 2 1 1 1 1 282.5 1 1 1 1 1 0 0 1 1 1

4574 2 1 1 1 2 1 1 1 1 266.4 1 1 1 1 1 0 0 1 1 1

4575 2 1 1 1 2 1 1 1 1 292.9 1 1 1 1 1 0 0 1 1 1

4576 2 1 1 1 2 1 1 1 1 262.1 1 1 1 1 1 0 0 1 1 1

4577 1 1 1 1 2 1 1 1 1 262.9 1 1 1 1 1 0 0 1 1 1

4578 1 1 1 1 2 1 1 1 1 269.5 1 1 1 1 1 0 0 1 1 1

4579 2 1 1 1 2 1 1 1 1 288.7 1 1 1 1 1 0 0 1 1 1

4580 2 1 1 1 2 1 1 1 1 291.3 1 2 4 1 1 0 0 1 1 1
Huntingdon Life Sciences 00-6130 Page 463

211-DIPE-S Final Report

Females

Individual Functional Observational Battery Evaluations

8th week of exposure Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group III – 10000 mg/m3

3561 1 1 1 1 2 1 955 845 900 900 900 900 1 6.1 5.2 5.7

3562 1 1 1 1 2 1 1245 1185 1215 680 505 593 1 5.0 4.7 4.9

3563 1 1 1 1 2 1 1490 1340 1415 595 915 755 1 5.2 3.6 4.4

3564 1 1 1 1 2 1 940 1370 1155 810 550 680 1 3.3 3.8 3.6

3565 1 1 1 1 2 1 1310 1255 1283 805 960 883 1 4.0 4.7 4.4

3566 1 1 1 1 2 1 1255 1285 1270 700 840 770 1 4.1 3.8 4.0

3567 1 1 1 1 2 1 760 1200 980 600 885 743 1 4.8 4.7 4.8

3568 1 1 1 1 2 1 990 930 960 985 710 848 1 6.2 5.6 5.9

3569 1 1 1 1 2 1 1440 1405 1423 805 1000 903 1 5.8 6.7 6.3

3570 1 1 1 1 2 1 775 995 885 1035 890 963 1 4.6 4.5 4.6

Group IV – 20000 mg/m3

4571 1 1 1 1 2 1 715 1195 955 615 465 540 1 7.1 6.7 6.9

4572 1 1 1 1 2 1 865 795 830 750 610 680 1 6.2 5.4 5.8

4573 1 1 1 1 2 1 1090 1335 1213 915 515 715 1 4.0 4.1 4.1

4574 1 1 1 1 2 1 750 920 835 690 615 653 1 5.3 5.0 5.2

4575 1 1 1 1 2 1 805 1215 1010 935 705 820 1 5.0 5.1 5.1

4576 1 1 1 1 2 1 1355 1110 1233 725 610 668 1 6.2 4.6 5.4

4577 1 1 1 1 2 1 1150 1390 1270 840 645 743 1 6.2 7.5 6.9

4578 1 1 1 1 2 1 810 845 828 840 1085 963 1 5.1 4.3 4.7

4579 1 1 1 1 2 1 1085 695 890 885 505 695 1 5.7 5.3 5.5

4580 1 1 1 1 2 1 1530 705 1118 995 880 938 1 5.3 6.6 6.0
Huntingdon Life Sciences 00-6130 Page 464

211-DIPE-S Final Report

Males

Individual Functional Observational Battery Evaluations

13th week of exposure Appendix H

a

Humane sacrificed.

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp 

Numbe Post Vocal Close Rem Hand Chromo Lac Sa Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group I - 0 mg/m3

1071 1 1 1 1 2 1 1 1 1 665.3 1 1 1 1 1 0 0 1 1 1

1072 1 1 1 1 2 1 1 1 1 543.7 1 1 1 1 1 6 3 1 1 1

1073 2 1 1 3 3 1 1 1 1 547.3 1 1 1 1 1 0 0 1 1 1

1074 1 1 1 1 2 1 1 1 1 464.1 1 1 1 1 1 4 3 1 1 1

1075 1 1 1 1 2 1 1 1 1 511.3 1 1 1 1 1 0 0 1 1 1

1076 1 1 1 1 2 1 1 1 1 635.0 1 1 1 1 1 0 4 1 1 1

1077 1 1 1 1 2 1 1 1 1 524.7 1 1 1 1 1 0 0 1 1 1

1078 1 1 1 1 2 1 1 1 1 524.6 1 1 1 1 1 0 1 1 1 1

1079 1 1 1 1 2 1 1 1 1 550.9 1 1 1 1 1 0 0 1 1 1

1080 2 1 1 1 2 1 1 1 1 588.9 1 1 1 1 1 0 2 1 1 1

Group II – 2000 mg/m3

2061 2 1 1 1 2 1 1 1 1 503.2 1 1 1 1 1 0 0 1 1 1

2062 1 1 1 1 2 1 1 1 1 552.4 1 1 1 1 1 0 0 1 1 1

2063 1 1 1 1 2 1 1 1 1 582.5 1 1 1 1 1 0 0 1 1 1

2064 1 1 1 1 2 1 1 1 1 462.5 1 1 1 1 1 6 4 1 1 1

2065 2 1 1 1 1 1 1 1 1 611.6 1 1 1 1 1 5 7 1 1 1

2066 1 1 1 1 2 1 1 1 1 589.0 1 1 1 1 1 4 0 1 1 1

2067 1 1 1 1 2 1 1 1 1 532.6 1 1 1 1 1 7 0 1 1 1

2068 2 1 1 2 2 1 1 1 1 601.1 1 1 1 1 1 2 0 1 1 1

2069 1 1 1 1 2 1 1 1 1 577.9 1 1 1 1 1 0 0 1 1 1

2070 a a a a a a a a a a a a a a a a a a a a
Huntingdon Life Sciences 00-6130 Page 465

211-DIPE-S Final Report

Males

Individual Functional Observational Battery Evaluations

13th week of exposure Appendix H

a

Humane sacrificed.

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approac Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group I - 0 mg/m3

1071 1 1 1 1 2 1 1830 1795 1813 1145 1345 1245 1 7.1 7.2 7.2

1072 1 1 1 1 2 1 1370 1000 1185 880 970 925 1 4.7 5.4 5.1

1073 1 1 1 1 2 1 1565 1400 1483 1440 1540 1490 1 7.7 8.0 7.9

1074 1 1 1 1 2 1 970 490 730 1025 715 870 1 7.5 6.7 7.1

1075 1 1 1 1 2 1 1050 1115 1083 835 745 790 1 10.5 7.9 9.2

1076 1 1 1 1 2 1 1080 1085 1083 830 905 868 1 4.5 4.2 4.4

1077 3 1 1 1 2 1 1340 1500 1420 770 840 805 1 7.0 6.4 6.7

1078 1 1 1 1 2 1 1390 1355 1373 1225 1105 1165 1 4.6 3.9 4.3

1079 1 1 1 1 2 1 1350 1550 1450 890 1030 960 1 5.6 4.7 5.2

1080 1 1 1 1 2 1 1295 1165 1230 945 880 913 1 6.8 5.4 6.1

Group II – 2000 mg/m3

2061 1 1 1 1 2 1 1165 1070 1118 1225 1135 1180 1 6.4 6.2 6.3

2062 1 1 1 1 2 1 1295 1170 1233 1095 1220 1158 1 7.1 6.8 7.0

2063 1 1 1 1 2 1 1480 1555 1518 805 920 863 1 6.6 9.4 8.0

2064 1 1 1 1 2 1 1395 1025 1210 790 625 708 1 6.8 6.4 6.6

2065 1 1 1 1 2 1 815 930 873 915 910 913 1 5.3 5.2 5.3

2066 1 1 1 1 2 1 925 1005 965 1040 790 915 1 6.3 5.2 5.8

2067 1 1 1 1 2 1 1185 1155 1170 685 930 808 1 5.3 5.4 5.4

2068 1 1 1 1 2 1 1535 1575 1555 1390 1255 1323 1 7.7 5.8 6.8

2069 1 1 1 1 2 1 1025 1230 1128 620 695 658 1 4.8 4.2 4.5

2070 a a a a a a a a a a a a a a a a
Huntingdon Life Sciences 00-6130 Page 466

211-DIPE-S Final Report

Males

Individual Functional Observational Battery Evaluations

13th week of exposure Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp 

Numbe Post Vocal Clos Rem Hand Chromo Lac Sa Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group III – 10000 mg/m3

3061 1 1 1 1 2 1 1 1 1 599.2 1 1 1 1 1 0 0 1 1 1

3062 1 1 1 1 2 1 1 1 1 597.1 1 1 1 1 1 0 7 1 1 1

3063 2 1 1 1 2 1 1 1 1 567.0 1 1 1 1 1 4 0 1 1 1

3064 1 1 1 1 2 1 1 1 1 594.3 1 1 1 1 1 2 0 1 1 1

3065 1 1 1 1 2 1 1 1 1 476.5 1 1 1 1 1 0 0 1 1 1

3066 1 1 1 1 2 1 1 1 1 533.5 1 1 1 1 1 0 0 1 1 1

3067 1 1 1 1 2 1 1 1 1 617.8 1 1 1 1 1 0 0 1 1 1

3068 1 1 1 2 2 1 1 1 1 626.7 1 1 1 1 1 0 0 1 1 1

3069 1 1 1 1 2 1 1 1 1 567.0 1 1 1 1 1 0 0 1 1 1

3070 1 1 1 1 2 1 1 1 1 601.7 1 1 1 1 1 7 0 1 1 1

Group IV – 20000 mg/m3

4071 1 1 1 1 2 1 1 1 1 515.7 1 1 1 1 1 0 0 1 1 1

4072 1 1 1 1 2 1 1 1 1 622.5 1 1 1 1 1 0 1 1 1 1

4073 1 1 1 1 2 1 1 1 1 569.2 1 1 1 1 1 0 0 1 1 1

4074 1 1 1 1 2 1 1 1 1 433.7 1 1 1 1 1 0 0 1 1 1

4075 1 1 1 1 2 1 1 1 1 517.8 1 1 1 1 1 0 1 1 1 1

4076 2 1 1 2 2 1 1 1 1 593.2 1 1 1 1 1 0 0 1 1 1

4077 2 1 1 1 2 1 1 1 1 514.2 1 1 1 1 1 3 3 1 1 1

4078 2 1 1 2 2 1 1 1 1 566.3 1 1 1 1 1 3 0 1 1 1

4079 1 1 1 3 3 1 1 1 1 585.0 1 1 1 1 1 0 0 1 1 1

4080 2 1 1 2 2 1 1 1 1 539.5 1 1 1 1 1 0 0 1 1 1
Huntingdon Life Sciences 00-6130 Page 467

211-DIPE-S Final Report

Males

Individual Functional Observational Battery Evaluations

13th week of exposure Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group III – 10000 mg/m3

3061 1 1 1 1 2 1 975 1300 1138 750 475 613 1 5.3 5.2 5.3

3062 1 1 1 1 2 1 1740 1000 1370 940 675 808 1 4.5 3.7 4.1

3063 2 1 1 1 2 1 1245 955 1100 1410 1340 1375 1 7.3 7.4 7.4

3064 1 1 1 1 2 1 1245 1520 1383 660 670 665 1 7.9 7.8 7.9

3065 1 1 1 1 2 1 1315 1220 1268 735 700 718 1 5.9 4.6 5.3

3066 1 1 1 1 2 1 1830 1020 1425 1370 1040 1205 1 6.2 5.3 5.8

3067 1 1 1 1 2 1 1475 915 1195 1290 1165 1228 1 4.3 5.3 4.8

3068 1 1 1 1 2 1 675 1130 903 990 1075 1033 1 6.4 6.1 6.3

3069 1 1 1 1 2 1 1375 850 1113 1005 485 745 1 6.3 4.3 5.3

3070 1 1 1 1 2 1 840 950 895 1390 1685 1538 1 5.0 5.3 5.2

Group IV – 20000 mg/m3

4071 1 1 1 1 2 1 1235 1340 1288 670 435 553 1 5.6 6.3 6.0

4072 1 1 1 1 2 1 1100 1235 1168 1485 1505 1495 1 5.8 4.9 5.4

4073 1 1 1 1 2 1 1195 890 1043 680 685 683 1 7.6 6.3 7.0

4074 1 1 1 1 2 1 1020 1175 1098 785 740 763 1 7.8 6.9 7.4

4075 1 1 1 1 2 1 1135 1235 1185 705 705 705 1 5.1 4.6 4.9

4076 1 1 1 1 2 1 1295 1570 1433 930 1160 1045 1 5.5 3.6 4.6

4077 1 1 1 1 2 1 1240 1095 1168 880 1100 990 1 4.9 5.2 5.1

4078 1 1 1 1 2 1 1925 1635 1780 320 655 488 1 5.5 4.1 4.8

4079 1 1 1 1 2 1 1525 1550 1538 1225 1250 1238 1 5.2 4.4 4.8

4080 1 1 1 1 2 1 1375 1415 1395 1020 1290 1155 1 6.8 7.5 7.2
Huntingdon Life Sciences 00-6130 Page 468

211-DIPE-S Final Report

Females

Individual Functional Observational Battery Evaluations

13th week of exposure Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp 

Numbe Post Vocal Clos Rem Hand Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams

Group I - 0 mg/m3

1571 1 1 1 1 2 1 1 1 1 329.8 1 1 1 1 1 0 0 1 1 1

1572 1 1 1 1 2 1 1 1 1 329.9 1 1 1 1 1 0 0 1 1 1

1573 2 1 1 1 2 1 1 1 1 376.2 1 1 1 1 1 0 1 1 1 1

1574 1 1 1 1 1 1 1 1 1 345.5 1 1 1 1 1 0 0 1 1 1

1575 1 1 1 1 2 1 1 1 1 272.8 1 1 1 1 1 3 1 1 1 1

1576 1 1 1 1 2 1 1 1 1 308.8 1 1 1 1 1 0 0 1 1 1

1577 1 1 1 1 2 1 1 1 1 296.2 1 1 1 1 1 0 0 1 1 1

1578 1 1 1 1 2 1 1 1 1 331.5 1 1 1 1 1 1 2 1 1 1

1579 1 1 1 1 2 1 1 1 1 392.9 1 1 1 1 1 2 2 1 1 1

1580 2 1 1 1 2 1 1 1 1 304.0 1 1 1 1 1 0 0 1 1 1

Group II – 2000 mg/m3

2561 2 1 1 1 2 1 1 1 1 275.3 1 1 1 1 1 0 0 1 1 1

2562 2 1 1 1 2 1 1 1 1 352.2 1 1 1 1 1 0 0 1 1 1

2563 1 1 1 1 1 1 1 1 1 317.6 1 1 1 1 1 0 1 1 1 1

2564 2 1 1 1 2 1 1 1 1 320.7 1 1 1 1 1 0 1 1 1 1

2565 2 1 1 1 2 1 1 1 1 325.3 1 1 1 1 1 0 1 1 1 1

2566 1 1 1 1 2 1 1 1 1 255.1 1 1 1 1 1 0 0 1 1 1

2567 1 1 1 2 2 1 1 1 1 305.0 1 1 1 1 1 0 0 1 1 1

2568 2 1 1 1 2 1 1 1 1 341.0 1 2 4 1 1 0 0 1 1 1

2569 2 1 1 1 2 1 1 1 1 321.3 1 1 1 1 1 0 0 1 1 1

2570 2 1 1 1 2 1 1 1 1 327.9 1 1 1 1 1 0 0 1 1 1
Huntingdon Life Sciences 00-6130 Page 469

211-DIPE-S Final Report

Females

Individual Functional Observational Battery Evaluations

13th week of exposure Appendix H

a

Slight tremors upon handling.

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group I - 0 mg/m3

1571 1 1 1 1 2 1 1400 1395 1398 1135 1085 1110 1 4.2 3.6 3.9

1572 1 1 1 1 2 1 1080 1600 1340 910 890 900 1 3.9 3.2 3.6

1573 1 1 1 1 2 1 1065 990 1028 575 660 618 1 4.2 4.6 4.4

1574 1 1 1 1 2 1 815 930 873 755 750 753 1 4.1 4.3 4.2

1575 1 1 1 1 2 1 675 875 775 725 785 755 1 6.0 5.8 5.9

1576 1 1 1 1 2 1 1095 1300 1198 765 865 815 1 4.2 3.9 4.1

1577 1 1 1 1 2 1 1055 1365 1210 870 800 835 1 3.6 3.4 3.5

1578 1 1 1 1 2 1 1535 665 1100 685 950 818 1 4.6 4.1 4.4

1579 1 1 1 1 2 1 670 1075 873 735 1050 893 1 4.4 3.2 3.8

1580 1 1 1 1 2 1 1260 1010 1135 1260 1305 1283 1 4.3 3.4 3.9

Group II – 2000 mg/m3

2561 1 1 1 1 2 1 850 975 913 815 830 823 1 3.5 4.2 3.9

2562 1 1 1 1 2 1 1010 1390 1200 860 855 858 1 5.6 3.5 4.6

2563 1 1 1 1 2 1 580 1525 1053 1005 915 960 1 4.7 4.5 4.6

2564 1 1 1 1 2 1 815 1115 965 1220 1000 1110 1 3.0 3.9 3.5

2565 1 1 1 1 2 1 1145 640 893 635 455 545 1 6.9 5.8 6.4

2566 1 1 1 1 2 1 1270 1210 1240 730 630 680 1 3.3 3.7 3.5

2567 1 1 1 1 2 1 935 560 748 560 670 615 1a 5.6 5.4 5.5

2568 1 1 1 1 2 1 740 850 795 520 750 635 1 5.3 4.4 4.9

2569 1 1 1 1 2 1 1145 1000 1073 800 615 708 1 4.6 4.1 4.4

2570 1 1 1 1 2 1 1515 1185 1350 730 1050 890 1 4.7 5.2 5.0
Huntingdon Life Sciences 00-6130 Page 470

211-DIPE-S Final Report

Females

Individual Functional Observational Battery Evaluations

13th week of exposure Appendix H

Home Cage Handling Open Field

Evaluations Evaluations Evaluations

Animal Palp 

Numbe Post Vocal Clos Rem Han Chromo Lac Sal Coat BW Gait Loco Arousal Exo Pilo Feces Urine Fasc Trem Conv

(grams)

Group III – 10000 mg/m3

3561 1 1 1 1 2 1 1 1 1 313.6 1 1 1 1 1 0 0 1 1 1

3562 1 1 1 1 2 1 1 1 1 263.7 1 1 1 1 1 0 3 1 1 1

3563 1 1 1 1 2 1 1 1 1 263.3 1 1 1 1 1 0 0 1 1 1

3564 1 1 1 1 2 1 1 1 1 334.9 1 1 1 1 1 0 0 1 1 1

3565 1 1 1 1 1 1 1 1 1 379.6 1 1 1 1 1 0 0 1 1 1

3566 1 1 1 1 2 1 1 1 1 298.8 1 1 1 1 1 0 0 1 1 1

3567 2 1 1 1 2 1 1 1 1 292.0 1 1 1 1 1 0 0 1 1 1

3568 1 1 1 1 2 1 1 1 1 317.6 1 1 1 1 1 0 0 1 1 1

3569 1 1 1 1 2 1 1 1 1 273.5 1 1 1 1 1 0 1 1 1 1

3570 1 1 1 1 2 1 1 1 1 309.8 1 1 1 1 1 4 1 1 1 1

Group IV – 20000 mg/m3

4571 1 1 1 1 2 1 1 1 1 356.4 1 1 1 1 1 0 1 1 1 1

4572 1 1 1 1 2 1 1 1 1 309.3 1 1 1 1 1 0 0 1 1 1

4573 1 1 1 1 2 1 1 1 1 309.8 1 1 1 1 1 0 0 1 1 1

4574 1 1 1 1 1 1 1 1 1 282.1 1 1 1 1 1 0 0 1 1 1

4575 2 1 1 1 2 1 1 1 1 312.5 1 1 1 1 1 0 0 1 1 1

4576 2 1 1 1 2 1 1 1 1 280.3 1 1 1 1 1 0 3 1 1 1

4577 2 1 1 1 2 1 1 1 1 287.2 1 1 1 1 1 0 0 1 1 1

4578 1 1 1 1 2 1 1 1 1 279.8 1 1 1 1 1 0 0 1 1 1

4579 1 1 1 1 2 1 1 1 1 319.0 1 1 1 1 1 0 0 1 1 1

4580 2 1 1 1 2 1 1 1 1 312.2 1 2 4 1 1 0 0 1 1 1
Huntingdon Life Sciences 00-6130 Page 471

211-DIPE-S Final Report

Females

Individual Functional Observational Battery Evaluations

13th week of exposure Appendix H

Reflex Grip Strength Landing Foot Splay

Assessments (grams) Air (centimeters)

Animal Visual Pupil Pinna Forelimb Hindlimb Righting

Number Approach Sound Pain Response Reflex Propri Trial 1 Trial 2 Mean Trial 1 Trial 2 Mean Reflex Trial 1 Trial 2 Mean

Group III – 10000 mg/m3

3561 1 1 1 1 2 1 825 1010 918 625 865 745 1 4.8 3.6 4.2

3562 1 1 1 1 2 1 1280 1245 1263 1090 1135 1113 1 5.2 3.9 4.6

3563 1 1 1 1 2 1 1015 1215 1115 725 535 630 1 4.1 3.2 3.7

3564 1 1 1 1 2 1 1030 1180 1105 900 900 900 1 4.0 4.9 4.5

3565 1 1 1 1 2 1 1265 940 1103 800 610 705 1 4.7 5.5 5.1

3566 1 1 1 1 2 1 1420 1020 1220 645 905 775 1 5.7 3.6 4.7

3567 1 1 1 1 2 1 1190 1570 1380 985 935 960 1 4.6 5.0 4.8

3568 1 1 1 1 2 1 1255 985 1120 900 725 813 1 5.6 5.3 5.5

3569 1 1 1 1 2 1 1190 1245 1218 1005 830 918 1 4.8 4.5 4.7

3570 1 1 1 1 2 1 1175 950 1063 965 910 938 1 5.4 4.7 5.1

Group IV – 20000 mg/m3

4571 1 1 1 1 2 1 765 740 753 590 820 705 1 5.6 5.7 5.7

4572 1 1 1 1 2 1 695 1390 1043 900 800 850 1 5.3 4.5 4.9

4573 1 1 1 1 2 1 1165 895 1030 795 775 785 1 5.6 4.6 5.1

4574 1 1 1 1 2 1 1520 1285 1403 650 800 725 1 4.9 4.6 4.8

4575 1 1 1 1 2 1 950 980 965 855 805 830 1 4.4 3.8 4.1

4576 1 1 1 1 2 1 1165 1295 1230 830 875 853 1 4.6 6.2 5.4

4577 1 1 1 1 2 1 1030 895 963 620 720 670 1 4.6 4.7 4.7

4578 1 1 1 1 2 1 850 940 895 895 1080 988 1 4.8 4.2 4.5

4579 1 1 1 1 2 1 1290 805 1048 830 820 825 1 4.3 5.1 4.7

4580 1 1 1 1 1 1 1030 1075 1053 800 825 813 1 5.7 5.4 5.6
Huntingdon Life Sciences 00-6130 Page 472

211-DIPE-S Final Report

Individual Hematology Values

Preface Appendix I

Reporting 

Abbreviation Parameter Units 

HGB Hemoglobin Concentration g/dL

HCT Hematocrit percent

RBC Erythrocyte Count 106

/μL 

RETIC Absolute Reticulocyte Count 109

/L

PLT Platelet Count 103

/μL 

MCV Mean Corpuscular Volume fL

MCH Mean Corpuscular Hemoglobin pg

MCHC Mean Corpuscular Hemoglobin

Concentration g/dL

WBC Total Leukocyte Count 103

/μL 

ANEU Absolute Neutrophils 103

/μL 

ALYM Absolute Lymphocytes 103

/μL 

AMONO Absolute Monocytes 103

/μL 

AEOS Absolute Eosinophils 103

/μL 

ABASO Absolute Basophils 103

/μL 

ALUC Absolute Large Unstained Cells 103

/μL 

ERYTHROCYTE MORPHOLOGY

Abbreviation Cell Morphology Range

Poly Polychromasia 1+ = few

Poik Poikilocytosis 2+ = slight

NAN = No

Abnormalities

Noted

Key to Other Abbreviations

CS = Clotted Specimen

NS = No Specimen 

Notes:

1. Animal 2060 at termination had no specimen because it was transferred to the Satellite 

Group.

2. Manual differential values were reported for Animal Nos. 1063M (Week 4), 1068M, 

4062M, 1568F, 2559F, 4563F, 4569F (Termination), and 1057M (Recovery) and 

therefore the value for ALUC is presented as 0. 
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Week 4 

Group 

1M 

0 mg/m(3) 

Animal 

Number 

1061 1062 1063 1064 1065 

1066 1067 1068 1069 1070 

Mean 

SD 

n 

HGB 

g/dL 

16.5 16.7 16.2 16.0 15.8 

16.3 

15.1 16.2 15.2 15.5 

16.0 

0.54 

10 

HCT 

% 

49.6 

50.2 

49.7 48.4 46.4 49.5 47.3 49.3 46.4 47.5 48.4 

1. 43 

10 

RBC 

9.00 

8.89 8.55 8.62 8.42 8.78 8.62 8.91 8.36 8.04 

8.62 

0.294 

10 

RETIC PLT MCV MCH MCHC 

fL pg g/dL 

188.2 922 55.1 18.3 33.2 

212.3 960 56.5 18.7 33.2 

187.9 840 58.1 19.0 32.7 

199.0 930 56.1 18.6 33.1 

137.1 738 55.1 18.7 34.0 

234.6 913 56.4 18.6 33.0 

193.1 899 54.8 17.6 32.0 

225.0 753 55.3 18.2 32.9 200.7 1258 55.5 18.2 32.9 246.0 809 59.0 19.3 32.7 

202.4 902 56.2 18.5 33.0 

30.41 146.4 1. 39 0.47 0.50 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Week 4 

Group 

1M 

o mg/m(3) 

Animal 

Number 

1061 1062 1063 1064 1065 1066 

1067 

1068 1069 

1070 

Mean 

SD 

n 

ANEU 

X10A3/uL 

1. 63 

2.76 

1. 66 1. 57 

2.12 2.63 

1. 68 1. 69 

2.04 

1. 65 

1. 94 

0.437 

10 

ALYM 

X10A3/uL 

10.86 13.38 14.14 15.02 15.66 

12.09 

9.94 

12.61 12.38 

16.06 

13.21 

2.022 

10 

AMONO 

X10A3/uL 

0.40 

0.48 0.67 0.32 0.35 0.50 

0.34 0.36 0.38 0.43 0.42 

0.105 

10 

AEOS ABA SO ALUC Poik Poly 

X10A3/UL X10A3/uL X10A3/uL 

0.12 0.12 0.07 NAN 1+ 0.09 0.18 0.10 NAN 1+ 

0.17 0.00 0.00 NAN 1+ 0.28 0.17 0.06 1+ 1+ 0.32 0.18 0.07 1+ 1+ 0.12 0.15 0.06 NAN 1+ 0.17 0.08 0.04 1+ 1+ 

0.31 0.16 0.09 NAN 1+ 0.39 0.12 0.07 1+ 1+ 0.20 0.20 0.16 1+ 1+ 0.22 0.14 0.07 

0.102 0.060 0.041 

10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - week 4 

Group 

2M 

2000 mg/m(3) 

Animal 

Number 

2051 2052 2053 2054 2055 

2056 2057 

2058 2059 2060 

Mean 

SD 

n 

HGB 

g/dL 

16.1 15.7 16.5 15.7 16.1 

15.2 

15.9 

15.5 

15.7 

16.0 15.8 

0.36 

10 

HCT 

% 

49.9 47.3 

50.6 

47.8 

49.7 

46.2 48.5 46.7 48.0 49.5 

48.4 

1.47 

10 

RBC 

x10A6/uL 

8.72 8.68 

9.03 

8.97 

9.02 

8.20 8.60 8.02 

8.69 

9.32 

8.73 

0.392 

10 

RETIC PLT MCV MCH MCHC 

X10A9/L X10A3/UL fL pg g/dL 

167.9 751 57.2 18.4 32.2 

168.3 922 54.5 18.1 33.1 

178.7 921 56.1 18.3 32.6 

174.0 1121 53.3 17.5 32.8 

215.3 690 55.1 17.9 32.5 

201.9 1309 56.3 18.6 33.0 

145.6 824 56.4 18.5 32.8 

178.3 683 58.3 19.3 33.2 

174.3 978 55.2 18.0 32.7 166.2 937 53.1 17.1 32.3 

177.1 914 55.6 18.2 32.7 

19.35 194.9 1. 65 0.61 0.33 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Week 4 

Group 

2M 

2000 mg/m(3) 

Animal 

Number 

2051 2052 2053 2054 2055 2056 2057 2058 

2059 2060 

Mean 

SD 

n 

ANEU 

x10A3/uL 

2.16 

1.20 

1.17 

1. 60 1. 51 

2.38 

1. 47 

2.11 2.83 

1.40 

1. 78 

0.555 

10 

ALYM 

Xl0A3/uL 

10.29 

12.44 14.68 

18.19 

9.94 

13.42 17.35 14.99 13.41 

14.88 

13.96 

2.674 

10 

AMONO 

x10A3/uL 

0.48 0.20 0.27 0.30 0.26 

0. 71 0.23 

0.36 0.43 0.40 

0.36 

0.152 

10 

AEOS ABA SO ALUC Poik Poly X10A3/uL X10A3/UL X10A3/UL 

0.18 0.14 0.09 NAN 1+ 

0.17 0.12 0.07 1+ 1+ 0.09 0.13 0.07 1+ 1+ 0.11 0.23 0.10 1+ 1+ 0.21 0.09 0.07 1+ 1+ 0.09 0.11 0.10 NAN 1+ 0.12 0.19 0.08 1+ 1+ 0.19 0.20 0.08 1+ 1+ 0.11 0.16 0.12 1+ 1+ 

0.22 0.19 0.15 1+ 1+ 

0.15 0.16 0.09 

0.050 0.045 0.026 

10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Week 4 

Group 

3M 

10000 mg/m (3) 

Animal 

Number 

3051 3052 3053 3054 3055 3056 

3057 

3058 3059 3060 

Mean 

SD 

n 

HGB 

g/dL 

15.8 15.6 16.2 15.6 15.0 

15.6 

14.7 15.7 15.7 

15.3 

15.5 

0.42 

10 

HCT 

% 

47.6 49.4 48.9 47.1 43.6 48.5 

43.7 47.7 48.5 47.0 47.2 

2.02 

10 

RBC x10A6/uL 

8.70 8.96 8.43 8.20 

7.86 

8.94 

7.58 

8.68 8.98 8.31 

8.46 

0.481 

10 

RETIC PLT MCV MCH MCHC 

X10A9/L X10A3/UL fL pg g/dL 

188.8 681 54.6 18.1 33.2 

213.6 1052 55.1 17.4 31.6 

160.9 1135 58.0 19.3 33.2 

172.6 917 57.4 19.0 33.1 

136.8 1178 55.5 19.1 34.5 

152.6 803 54.2 17.5 32.2 182.9 809 57.6 19.4 33.6 147.9 701 55.0 18.1 32.8 

139.4 1214 54.0 17.5 32.5 

192.7 658 56.5 18.5 32.7 

168.8 915 55.8 18.4 32.9 

25.48 215.0 1.48 0.78 0.79 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Week 4 

Group 

3M 

10000 mg/m(3) 

Animal 

Number 

3051 3052 

3053 

3054 3055 3056 3057 3058 3059 

3060 

Mean 

SD 

n 

ANEU 

X10A3/uL 

2.56 

1.46 

2.22 

0.99 0.78 

1. 90 

1. 86 1.55 

1. 04 

2.30 

1.67 

0.605 

10 

ALYM 

x10A3/uL 

17.10 

14.38 

16.80 15.16 

8.03 

14.77 

17.58 

12.78 14.71 15.61 14.69 

2.744 

10 

AMONO AEOS ABA SO ALUC Poik Poly 

x10A3/uL X10A3/UL x10A3/uL X10A3/UL 

0.25 0.50 0.22 0.13 1+ 1+ 0.19 0.05 0.15 0.11 1+ 1+ 0.68 0.18 0.19 0.11 1+ 1+ 0.27 0.07 0.15 0.11 1+ 1+ 0.17 0.04 0.05 0.04 1+ 1+ 0.58 0.19 0.17 0.09 1+ 1+ 0.44 0.11 0.15 0.11 1+ 1+ 0.38 0.21 0.15 0.12 1+ 1+ 0.40 0.06 0.11 0.17 1+ 1+ 0.49 0.24 0.15 0.07 1+ 1+ 

0. 39 0.17 0.15 0.11 

0.168 0.139 0.045 0.035 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Week 4 

Group 

4M 

20000 mg/m(3) 

Animal 

Number 

4061 4062 4063 

4064 4065 4066 

4067 

4068 

4069 

4070 Mean 

SD 

n 

HGB 

g/dL 

15.7 

16.2 15.3 15.3 

15.1 

15.8 

15.6 

14.9 

14.4 15.2 

15.4 

0.51 

10 

HCT 

% 

47.7 

50.0 

46.3 46.5 45.4 

49.1 46.7 46.1 44.2 47.4 

46.9 

1. 70 

10 

RBC 

X10A6/uL 

8.55 

9.28 

8.31 8.44 8.29 8.91 8.46 8.37 8.46 8.44 

8.55 

0.309 

10 

RETIC PLT MCV MCH MCHC 

x10A9/L X10A3/UL fL pg g/dL 

158.7 1190 55.8 18.3 32.8 

118.4 693 53.9 17.5 32.5 

139.7 1335 55.8 18.4 33.0 

169.7 675 55.1 18.2 33.0 

146.8 1023 54.8 18.3 33.3 

192.6 731 55.1 17.7 32.2 155.1 625 55.2 18.4 33.3 

203.2 698 55.1 17.8 32.3 

133.2 1370 52.2 17.0 32.5 140.8 961 56.2 18.0 32.0 

155.8 930 54.9 18.0 32.7 

26.41 287.2 1.15 0.46 0.46 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Week 4 

Group 

4M 

20000 mg/m{3) 

Animal 

Number 

4061 

4062 

4063 4064 

4065 4066 4067 

4068 

4069 

4070 

Mean 

SD 

n 

ANEU 

1. 78 

3.93 

1. 35 

2.30 

1.36 

2.28 

1.41 

2.70 

1.65 1. 39 

2.02 

0.824 

10 

ALYM 

9. 96 

9.04 

13.00 

15.02 10.28 

14.03 10.24 

13.46 

10.34 12.73 

11.81 

2.062 

10 

AMONO 

0.33 

0.40 0.49 

0.69 0.19 0.34 0.16 0.28 

0.23 0.23 

0.33 

0.160 

10 

AEOS ABA SO ALUC Poik Poly 

0.05 0.09 0.04 1+ 1+ 0.43 0.15 0.07 1+ 1+ 0.13 0.11 0.09 1+ 1+ 

0.44 0.19 0.16 1+ 1+ 0.17 0.10 0.07 1+ 1+ 0.13 0.16 0.12 1+ 1+ 0.45 0.09 0.06 1+ 1+ 

0.40 0.15 0.14 1+ 1+ 0.04 0.10 0.14 1+ 1+ 0.09 0.11 0.08 1+ 1+ 

0.23 0.13 0.10 

0.174 0.035 0.040 

10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - week 4 

Group 

1F 

0 mg/m(3) 

Animal 

Number 

1561 1562 1563 1564 

1565 

1566 1567 

1568 1569 1570 

Mean 

SD 

n 

HGB 

g/dL 

15.8 16.5 15.6 

15.3 

15.2 16.0 16.0 15.4 15.2 15.0 

15.6 

0.47 

10 

HCT 

% 

46.5 49.9 

46.0 

45.7 46.1 

47.5 47.5 46.1 

45.2 

45.2 

46.6 

1.42 

10 

RBC 

x10-6/uL 

8.42 

8.92 8.57 8.39 8.23 

8.49 8.55 

7.95 

8.05 8.21 

8.38 

0.282 

10 

RETIC PLT MCV MCH MCHC 

x10-9/L x10-3/uL fL pg g/dL 

171.9 1122 55.2 18.8 34.0 

220.3 984 55.9 18.5 33.2 

196.3 1161 53.6 18.2 34.0 

174.6 728 54.5 18.3 33.5 

170.9 1208 56.0 18.5 33.0 

222.0 891 56.0 18.8 33.7 267.8 846 55.5 18.7 33.7 

153.4 916 57.9 19.4 33.5 

185.4 1070 56.1 18.8 33.5 181.1 1069 55.0 18.3 33.2 

194.4 1000 55.6 18.6 33.5 

33.69 153.0 1.14 0.35 0.33 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Week 4 

Group 

1F 

o mg/m(3) 

Animal 

Number 

1561 1562 1563 1564 1565 1566 1567 

1568 1569 1570 

Mean 

SD 

n 

ANEU 

1. 89 1.55 1. 45 

2.21 

1. 36 

2.41 2.26 

1. 32 1. 88 1.45 1. 78 

0.407 

10 

ALYM 

16.08 13.58 17.17 14.47 10.90 15.24 

15.15 

8.35 

12.18 

8.38 

13.15 

3.106 

10 

AMONO AEOS ABASO ALUC Poik Poly 

0.52 0.13 0.21 0.21 1+ 1+ 0.41 0.11 0.18 0.09 1+ 1+ 

0.49 0.18 0.16 0.14 1+ 1+ 0.35 0.17 0.14 0.11 1+ 1+ 0.34 0.07 0.09 0.07 1+ 1+ 0.35 0.30 0.21 0.11 1+ 1+ 0.46 0.27 0.22 0.09 1+ 1+ 0.30 0.12 0.08 0.07 1+ 1+ 0.37 0.10 0.09 0.07 1+ 1+ 

0.32 0.09 0.08 0.07 1+ 1+ 0.39 0.15 0.15 0.10 

0.075 0.077 0.058 0.044 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Week 4 

Group 

2F 2000 mg/m(3) 

Animal 

Number 

2551 2552 2553 2554 2555 2556 2557 2558 2559 2560 

Mean 

SD 

n 

HGB 

g/dL 

15.6 

15.6 16.8 16.2 16.3 16.2 14.5 14.8 17.3 15.6 

15.9 

0.85 

10 

HCT 

% 

47.8 45.8 

50.3 50.4 

47.6 49.1 41.8 44.2 

54.5 

47.4 

47.9 

3.54 

10 

RBC RETIC 

X10A6/uL x10A9/L 

8.54 169.0 

8.80 143.9 

9.12 228.2 9.21 164.3 

8.49 160.0 

8.69 186.9 

7.20 219.1 

8.41 181.3 

9.88 223.6 

8.76 219.8 

8. 71 189.6 

0.687 30.79 

10 10 

PLT MCV MCH MCHC 

X10A3/uL fL pg g/dL 

1136 56.0 18.2 32.5 

1194 52.1 17.7 34.0 

919 55.1 18.5 33.5 911 54.7 17.6 32.2 933 56.1 19.2 34.2 791 56.5 18.6 33.0 

1018 58.0 20.2 34.8 1284 52.6 17.6 33.5 

837 55.1 17.5 31.7 

668 54.1 17.8 32.9 

969 55.0 18.3 33.2 

190.6 1.78 0.87 0.96 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Week 4 

Group 

2F 

2000 mg/m (3) 

Animal 

Number 

2551 2552 

2553 2554 

2555 

2556 

2557 2558 2559 

2560 

Mean 

SD 

n 

ANEU 

Xl0A3/UL 

0.77 0.82 

1. 30 1. 53 1.17 1.48 1.66 

2.12 

1. 31 

1. 82 

1. 40 

0.420 

10 

ALYM 

Xl0A3/uL 

14.50 

9.58 

13.69 15.46 

12.23 

10.81 

10.73 

14.38 11.58 14.99 12.80 

2.068 

10 

AMONO 

xl0A3/uL 

0.24 0.26 

0.18 0.34 0.27 

0.33 0.34 

0.34 0.18 0.57 0.31 

0.112 

10 

AEOS ABASO ALUC Poik Poly 

Xl0A3/uL Xl0A3/UL Xl0A3/UL 

0.11 0.13 0.13 1+ 1+ 0.10 0.08 0.12 1+ 1+ 0.07 0.15 0.10 1+ 1+ 

0.10 0.17 0.09 1+ 1+ 

0.12 0.10 0.08 1+ 1+ 0.16 0.09 0.09 1+ 1+ 0.42 0.11 0.07 1+ 1+ 

0.69 0.18 0.14 1+ 1+ 0.11 0.10 0.07 1+ 1+ 0.24 0.16 0.12 1+ 1+ 0.21 0.13 0.10 

0.197 0.036 0.025 

10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Week 4 

Group 

3F 

10000 mg/m(3) 

Animal 

Number 

3551 3552 3553 3554 3555 

3556 3557 

3558 3559 3560 

Mean 

SD 

n 

HGB 

g/dL 

15.7 

14.6 15.4 14.7 

15.8 

15.5 

15.7 14.7 15.9 14.4 

15.2 

0.57 

10 

HCT 

% 

47.2 44.1 46.1 43.0 48.5 

46.2 48.2 44.4 46.8 

42.0 

45.7 

2.19 

10 

RBC RETIC 

8.63 158.7 

7.92 217.1 

8.42 170.5 

7.36 257.8 

8.60 181.2 8.31 137.0 

8.66 224.8 

8.03 162.1 8.36 184.4 

7.50 195.3 

8.18 188.9 

0.464 35.92 

10 10 

PLT MCV MCH MCHC 

fL pg g/dL 

1184 54.7 18.2 33.3 1152 55.7 18.4 33.0 1336 54.8 18.3 33.3 

859 58.4 19.9 34.1 

421 56.4 18.4 32.6 

661 55.6 18.7 33.6 

1071 55.7 18.1 32.5 

794 55.3 18.3 33.2 759 56.0 19.0 34.0 

1276 55.9 19.1 34.3 

951 55.9 18.6 33.4 

297.8 1. 04 0.55 0.61 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Week 4 

Group 

3F 

10000 mg/m(3) 

Animal 

Number 

3551 3552 3553 3554 3555 3556 3557 3558 3559 3560 

Mean 

SD 

n 

ANEU 

xlOh3/uL 

1. 85 

2.07 

1. 69 1. 45 

2.35 2.96 

1. 51 1. 31 1. 32 1. 40 

1.79 

0.535 

10 

ALYM 

xlOh3/uL 

14.37 11.49 10.38 

9.97 

18.76 

15.18 13.07 

9.57 

12.53 

13.35 12.87 

2.790 

10 

AMONO AEOS ABASO ALUC Poik Poly 

xlOh3/uL xlOh3/uL xlOh3/uL xlOh3/uL 

0.23 0.10 0.15 0.11 1+ 1+ 0.54 0.08 0.09 0.09 1+ 1+ 0.17 0.08 0.09 0.08 1+ 1+ 0.24 0.14 0.07 0.05 1+ 1+ 0.29 0.56 0.21 0.17 1+ 1+ 0.47 0.48 0.18 0.12 1+ 1+ 0.35 0.11 0.11 0.07 1+ 1+ 0.34 0.13 0.11 0.10 1+ 1+ 0.29 0.39 0.13 0.09 1+ 1+ 0.20 0.06 0.09 0.12 1+ 1+ 

0.31 0.21 0.12 0.10 

0.118 0.188 0.045 0.033 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Week 4 

Group 

4F 

20000 mg/m(3) 

Animal 

Number 

4561 

4562 4563 

4564 4565 4566 

4567 

4568 

4569 

4570 

Mean 

SD 

n 

HGB 

g/dL 

13.5 

14.7 

15.3 15.8 

15.6 

15.2 14.7 15.2 16.0 15.2 

15.1 

0. 71 

10 

HCT 

% 

39.6 

43.8 47.5 

47.5 45.6 45.7 43.8 44.8 48.4 45.2 45.2 

2.51 

10 

RBC 

Xl0A6/uL 

7.08 

8.10 8.15 

8.62 8.32 8.78 

7.64 

8.52 8.78 

8.73 

8.27 

0.555 

10 

RETIC PLT MCV MCH MCHC 

X10A9/L X10A3/uL fL pg g/dL 

239.9 1086 55.9 19.0 34.0 

205.7 1142 54.0 18.2 33.6 

198.1 738 58.3 18.8 32.2 

187.2 1368 55.0 18.3 33.3 

177.9 956 54.9 18.7 34.1 

142.8 1273 52.1 17.3 33.2 

195.7 977 57.3 19.2 33.6 

113.0 789 52.6 17.8 33.9 

221.3 815 55.1 18.2 33.0 

169.2 1333 51.8 17.4 33.5 

185.1 1048 54.7 18.3 33.4 

36.99 229.9 2.15 0.65 0.56 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Week 4 

Group 

4F 

20000 mg/m(3) 

Animal 

Number 

4561 4562 

4563 

4564 

4565 4566 4567 4568 4569 4570 

Mean 

SD 

n 

ANEU 

1. 31 1. 77 1. 56 

0.82 

1. 06 

2. 71 

2.39 

2.47 

1. 33 

0.84 

1. 63 

0.689 

10 

ALYM 

9.12 

13.95 

9.37 

12.28 

13.39 12.16 14.36 13.01 

8.58 

12.60 11.88 

2. 096 

10 

AMONO AEOS ABASO ALUC Poik Poly 

0.19 0.07 0.06 0.05 1+ 1+ 0.21 0.13 0.16 0.09 1+ 1+ 0.24 0.44 0.09 0.07 1+ 1+ 0.08 0.03 0.11 0.10 1+ 1+ 0.30 0.22 0.11 0.07 1+ 1+ 0.35 0.10 0.12 0.10 1+ 1+ 0.32 0.24 0.13 0.15 1+ 1+ 0.43 0.28 0.12 0.07 1+ 1+ 0.20 0.35 0.09 0.05 1+ 1+ 0.19 0.07 0.09 0.14 1+ 1+ 0.25 0.19 0.11 0.09 

0.100 0.136 0.027 0.034 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Termination 

Group 

1M 

0 mg/m(3) 

Animal 

Number 

1061 1062 

1063 

1064 

1065 

1066 1067 1068 1069 1070 

Mean 

SD 

n 

HGB HCT 

g/dL % 

16.6 48.6 16.6 48.2 15.7 48.8 

16.2 47.4 16.4 48.5 

16.1 48.2 

15.0 44.4 17.0 50.4 

16.7 49.7 

16.0 47.1 16.2 48.1 

0.58 1. 63 

10 10 

RBC 

X10A6/uL 

9.55 9.18 9.06 9.05 9.27 9.29 

8.76 

9.65 9.37 

8.41 

9.16 

0.367 

10 

RETIC PLT MCV MCH MCHC 

x10A9/L X10A3/UL fL pg g/dL 

160.8 755 50.9 17.4 34.1 170.4 850 52.5 18.1 34.5 146.6 786 53.9 17.4 32.3 

158.9 909 52.4 17.9 34.2 

216.4 725 52.3 17.7 33.9 203.6 398 51.9 17.3 33.4 

188.0 1376 50.7 17.1 33.7 

205.2 562 52.2 17.7 33.8 

194.5 1137 53.0 17.8 33.5 

247.1 1055 56.0 19.0 33.9 

189.2 855 52.6 17.7 33.7 

30.69 282.9 1. 52 0.54 0.60 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Termination 

Group 

1M 

0 mg/m(3) 

Animal 

Number 

1061 1062 1063 1064 1065 

1066 1067 

1068 1069 1070 

Mean 

SD 

n 

ANEU ALYM X10A3/uL X10A3/UL 

2.10 9.00 

1. 91 8.12 

3.30 9.58 3.33 12.17 

1. 88 14.25 

3.07 6.92 

2.17 6.57 

1.45 6.29 1. 93 9.08 1. 50 9.42 

2.26 9.14 

0.709 2.501 

10 10 

AMONO AEOS ABASO ALUC Poik Poly 

X10A3/UL Xl0A3/uL x10A3/uL x10A3/UL 

0.35 0.22 0.11 0.04 1+ 1+ 0.26 0.18 0.07 0.02 NAN 1+ 0.50 0.13 0.11 0.05 1+ 1+ 0.35 0.14 0.12 0.06 1+ 1+ 0.37 0.41 0.20 0.10 1+ 1+ 

0.25 0.35 0.32 0.03 1+ 1+ 0.22 0.17 0.04 0.02 1+ 1+ 0.24 0.08 0.00 0.00 1+ 1+ 

0.47 0.14 0.08 0.09 NAN 1+ 0.24 0.11 0.06 0.07 NAN 1+ 

0.33 0.19 0.11 0.05 

0.100 0.107 0.091 0.032 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Termination 

Group 

2M 

2000 mg/m(3) 

Animal 

Number 

2051 2052 2053 2054 2055 2056 

2057 

2058 

2059 2060 

Mean 

SD 

n 

HGB HCT 

g/dL % 

16.0 47.2 15.2 44.0 16.6 48.4 

16.7 50.1 

16.5 48.0 16.1 47.6 

16.4 47.9 16.8 49.1 15.5 47.5 

NS NS 

16.2 47.8 

0.55 1. 67 

9 9 

RBC RETIC 

9.01 137.3 

8.58 185.7 

9.12 177.7 9.99 148.5 9.46 160.9 

8.96 187.7 

8.97 169.2 

9.25 195.9 

9.00 166.0 

NS NS 

9.15 169.9 

0.395 19.11 

9 9 

PLT MCV MCH MCHC 

fL pg g/dL 

1177 52.4 17.7 33.8 1243 51.2 17.8 34.7 1136 53.1 18.2 34.4 1119 50.2 16.7 33.3 

760 50.7 17.4 34.3 

1220 53.2 17.9 33.7 

1180 53.4 18.3 34.3 

1125 53.1 18.2 34.2 1169 52.8 17.2 32.5 

NS NS NS NS 

1125 52.2 17.7 33.9 

143.2 1. 21 0.53 0.68 

9 9 9 9 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Termination 

Group 

2M 

2000 mg/m(3) 

Animal 

Number 

2051 2052 2053 2054 2055 2056 2057 2058 2059 2060 

Mean 

SD 

n 

ANEU ALYM 

4.15 10.18 

1. 87 8.56 

2.30 11.67 

1. 66 18.80 1. 75 6.41 

1.35 9.02 1. 05 6.70 1. 90 9.81 

2.83 13.70 

NS NS 

2.10 10.54 

0.925 3.844 

9 9 

AMONO 

0.37 0.26 0.18 0.36 0.14 0.33 0.10 0.48 0.59 

NS 

0.31 

0.161 

9 

AEOS ABA SO ALUC Poik Poly 

0.08 0.14 0.06 1+ 1+ 

0.09 0.07 0.05 1+ 1+ 0.10 0.12 0.07 NAN 1+ 

0.25 0.20 0.20 1+ 1+ 0.19 0.05 0.03 NAN 1+ 0.15 0.06 0.06 1+ 1+ 0.11 0.04 0.04 1+ 1+ 0.27 0.10 0. 06 1+ 1+ 0.16 0.19 0.13 1+ 1+ 

NS NS NS NS NS 

0.16 0.11 0.08 

0.069 0.059 0.054 

9 9 9 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Termination 

Group 

3M 

10000 mg/m(3) 

Animal 

Number 

3051 3052 

3053 

3054 3055 3056 3057 3058 3059 3060 

Mean 

SD 

n 

HGB HCT 

g/dL % 

17.0 48.9 

13.9 49.0 15.9 46.4 16.6 49.8 15.9 45.8 16.9 50.4 14.5 41.9 

14.4 42.7 

15.6 45.4 

16.1 47.4 

15.7 46.8 

1. 08 2.90 

10 10 

RBC RETIC 

9.50 176.4 9.61 203.6 

8.67 169.7 

9.23 191.5 

8.79 141.0 

9.85 169.8 

7.82 182.1 

8.12 464.2 

9.33 143.2 

8.80 178.0 8.97 202.0 

0.655 94.13 

10 10 

PLT MCV MCH MCHC 

fL pg g/dL 

1242 51.5 17.9 34.7 

940 51.0 14.5 28.4 

1306 53.5 18.4 34.3 

953 53.9 18.0 33.3 

1366 52.1 18.0 34.7 

838 51.2 17.2 33.6 

913 53.5 18.5 34.6 

1193 52.7 17.7 33.6 1353 48.6 16.8 34.5 

1129 53.9 18.3 34.1 

1123 52.2 17.5 33.6 

197.8 1. 68 1.19 1. 89 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Termination 

Group 

3M 

10000 mg/m(3) 

Animal 

Number 

3051 3052 

3053 

3054 3055 

3056 

3057 

3058 3059 3060 

Mean 

SD 

n 

ANEU ALYM 

xl0A3/uL Xl0A3/uL 

1.92 10.73 

2.05 9.81 2.01 9.57 

1. 50 10.44 

1. 57 5.06 

1. 78 9.70 

3.03 8.64 

1. 38 6.98 

0.86 8.30 

1. 55 8.82 

1. 77 8.81 

0.566 1. 713 

10 10 

AMONO 

Xl0A3/UL 

0.16 0.17 0.42 

0.29 

0.19 0.21 0.73 0.15 

0.21 0.21 0.27 

0.179 

10 

AEOS ABA SO ALUC Poik Poly 

Xl0A3/uL Xl0A3/uL Xl0A3/uL 

0.23 0.09 0.04 1+ 1+ 0.11 0.08 0.07 NAN 1+ 

0.37 0.09 0.04 NAN 1+ 

0.10 0.09 0.06 NAN 1+ 0.08 0.03 0.02 1+ 1+ 

0.10 0.08 0.05 NAN 1+ 

0.10 0.07 0.04 1+ 1+ 

0.12 0.06 0.04 1+ 1+ 0.10 0.05 0.05 1+ 1+ 0.10 0.07 0.05 NAN 1+ 0.14 0.07 0.05 

0.091 0.020 0.013 

10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Termination 

Group 

4M 

20000 mg/m(3) 

Animal 

Number 

4061 4062 

4063 

4064 4065 4066 4067 4068 4069 

4070 

Mean 

SD 

n 

HGB HCT 

g/dL % 

16.5 48.7 17.5 52.5 16.5 49.8 16.7 49.3 15.2 44.4 15.8 48.4 15.7 46.2 15.7 48.0 14.8 42.9 15.7 46.9 16.0 47.7 

0.79 2.76 

10 10 

RBC 

9.38 

10.23 

9.51 9.49 

8.51 

9.42 

8.89 

9.27 

8.79 

9.09 

9.26 

0.477 

10 

RETIC PLT MCV MCH MCHC 

fL pg g/dL 

132.9 889 51.9 17.7 34.0 

160.5 622 51.4 17.1 33.4 161.1 1248 52.4 17.3 33.0 119.4 1016 51.9 17.6 33.9 

146.3 801 52.2 17.9 34.3 

184.2 934 51.4 16.7 32.6 

143.4 1309 52.0 17.7 34.1 

167.8 1035 51.8 16.9 32.6 148.4 1386 48.8 16.9 34.6 136.8 1196 51.6 17.3 33.6 

150.1 1044 51.5 17.3 33.6 

18.81 241.8 1. 01 0.41 0.70 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Termination 

Group 

4M 

20000 mg/m(3) 

Animal 

Number 

4061 

4062 4063 4064 4065 

4066 4067 4068 4069 

4070 

Mean 

SD 

n 

ANEU ALYM 

1. 62 8.81 

3.13 7.35 

2.09 10.99 

1. 63 15.55 1.18 7.18 

1. 54 9. 36 1. 52 8.08 

1. 91 8.30 

1. 45 6.11 

1. 94 7.76 

1. 80 8.95 

0.537 2. 672 

10 10 

AMONO AEOS ABASO ALUC Poik Poly 

0.27 0.40 0.09 0.03 1+ 1+ 0.11 0.22 0.00 0.00 NAN 1+ 

0.35 0.21 0.10 0.07 NAN 1+ 0.70 0.26 0.20 0.15 NAN 1+ 0.17 0.11 0.06 0.03 1+ 1+ 

0.24 0.39 0.08 0.06 1+ 1+ 0.18 0.10 0.06 0.07 1+ 1+ 0.34 0.11 0.09 0.06 NAN 1+ 0.17 0.08 0.04 0.05 NAN 1+ 0.25 0.08 0.06 0.06 NAN 1+ 

0.28 0.20 0.08 0.06 

0.167 0.122 0.052 0.039 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Termination 

Group 

1F 

0 mg/m(3) 

Animal 

Number 

1561 1562 1563 

1564 1565 

1566 1567 1568 1569 1570 

Mean 

SD 

n 

HGB HCT 

g/dL % 

16.1 46.7 15.4 44.5 

15.6 46.4 15.6 45.6 15.2 44.5 

15.5 44.5 15.9 46.3 

15.3 44.7 

16.4 48.2 14.8 43.4 

15.6 45.5 

0.46 1.42 

10 10 

RBC RETIC 

8.29 140.5 

8.28 212.8 8.86 139.7 8.54 161.1 8.13 151.6 8.08 216.3 8.37 201.1 8.03 143.2 8.66 178.2 8.08 168.2 

8.33 171.3 

0.278 29.67 

10 10 

PLT MCV MCH MCHC 

fL pg g/dL 

804 56.4 19.4 34.4 

1393 53.7 18.6 34.6 

1021 52.4 17.6 33.7 

955 53.4 18.2 34.2 

1295 54.8 18.6 34.0 

906 55.0 19.1 34.8 

830 55.3 19.0 34.3 

725 55.6 19.0 34.2 

1243 55.7 18.9 33.9 1069 53.7 18.2 34.0 

1024 54.6 18.7 34.2 

224.5 1. 25 0.54 0.33 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Termination 

Group 

1F 

0 mg/m(3) 

Animal 

Number 

1561 1562 

1563 

1564 

1565 1566 1567 1568 1569 1570 

Mean 

SD 

n 

ANEU ALYM 

1. 89 9.26 

4.30 6.74 

1. 80 8.78 1. 00 7.29 1. 71 5.62 

2.90 9.36 2.22 10.72 

1.15 3.20 

0.81 10.22 0.98 4.17 

1.88 7.54 

1.070 2.575 

10 10 

AMONO AEOS ABA SO ALUC Poik Poly 

0.22 0.18 0.07 0.06 1+ 1+ 0.39 0.10 0.04 0.05 1+ 1+ 0.25 0.26 0.07 0.04 1+ 1+ 0.19 0.12 0.05 0.04 1+ 1+ 0.17 0.07 0.05 0.03 2+ 1+ 0.35 0.38 0.10 0.09 NAN 1+ 0.38 0.14 0.07 0.05 NAN 1+ 0.04 0.04 0.00 0.00 1+ 1+ 0.29 0.16 0.06 0.06 NAN 1+ 0.10 0.09 0.03 0.02 NAN 1+ 0.24 0.15 0.05 0.04 

0.117 0.101 0.027 0.025 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Termination 

Group 

2F 

2000 mg/m(3) 

Animal 

Number 

2551 2552 2553 2554 2555 2556 2557 2558 2559 2560 

Mean 

SD 

n 

HGB HCT 

g/dL % 

16.0 47.2 16.5 47.8 16.4 48.3 16.4 49.4 16.7 46.9 16.8 48.1 14.2 40.8 15.2 44.0 17.4 53.6 15.7 46.1 

16.1 47.2 

0.91 3.34 

10 10 

RBC 

8.60 

9.31 

8.93 

9.03 

8.46 8.58 

7.18 

8.46 

9.68 

8.66 

8.69 

0.662 

10 

RETIC PLT MCV MCH MCHC 

fL pg g/dL 

154.1 903 55.0 18.6 33.9 150.6 918 51.4 17.7 34.4 181.6 955 54.1 18.4 34.0 176.4 971 54.6 18.2 33.3 155.0 800 55.4 19.8 35.7 

189.3 712 56.0 19.5 34.9 

178.7 1110 56.8 19.7 34.7 152.8 989 52.0 18.0 34.6 

220.9 678 55.3 18.0 32.5 

157.8 1173 53.2 18.2 34.1 

171.7 921 54.4 18.6 34.2 

22.32 158.2 1. 73 0.77 0.88 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Termination 

Group 

2F 2000 mg/m(3) 

Animal 

Number 

2551 

2552 2553 

2554 

2555 2556 

2557 

2558 2559 2560 

Mean 

SD 

n 

ANEU ALYM 

X10A3/UL X10A3/uL 

1. 49 11.11 1. 01 5.56 

0.73 7.91 

1.11 9.24 

0.76 7.58 

1. 07 7.04 

0.87 5.05 0.88 7.86 

1.66 8.50 

0.97 11.10 

1. 06 8.10 

0.303 2.021 

10 10 

AMONO AEOS ABASO ALUC Poik Poly 

X10A3/UL X10A3/UL x10A3/uL X10A3/uL 

0.22 0.16 0.09 0.07 NAN 1+ 0.12 0.16 0.04 0.03 1+ 1+ 0.16 0.05 0.05 0.04 NAN 1+ 0.18 0.16 0.07 0.05 NAN 1+ 0.15 0.14 0.05 0.04 NAN 1+ 0.20 0.18 0.05 0.04 NAN 1+ 0.14 0.05 0.03 0.02 NAN 1+ 0.15 0.14 0.06 0.05 1+ 1+ 0.10 0.10 0.00 0.00 1+ 1+ 

0. 38 0.18 0.07 0.12 1+ 1+ 

0.18 0.13 0.05 0.05 

0.079 0.049 0.025 0.032 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Termination 

Group 

3F 

10000 mg/m(3) 

Animal 

Number 

3551 3552 3553 

3554 

3555 3556 3557 

3558 3559 3560 

Mean 

SD 

n 

HGB HCT 

g/dL % 

15.8 45.4 

16.5 49.0 

16.0 46.1 

14.7 42.9 16.1 46.2 16.4 47.6 

15.9 46.6 15.5 44.1 

16.2 46.0 

16.1 45.6 

15.9 46.0 

0.52 1. 69 

10 10 

RBC x10A6/uL 

8.53 8.96 8.49 

7.40 

8.46 8.67 

8.41 8.05 8.45 

8.50 

8.39 

0.415 

10 

RETIC PLT MCV MCH MCHC x10A9/L X10A3/uL fL pg g/dL 

137.2 1000 53.3 18.5 34.7 

234.9 1076 54.7 18.4 33.6 

146.8 1419 54.3 18.8 34.6 

209.9 1006 58.0 19.8 34.2 205.0 686 54.6 19.0 34.9 

167.9 832 54.9 18.9 34.5 

205.9 935 55.4 18.9 34.0 

174.7 803 54.8 19.3 35.2 

133.6 1005 54.5 19.2 35.2 180.5 1368 53.6 18.9 35.3 

179.6 1013 54.8 19.0 34.6 

34.10 232.1 1.28 0.40 0.56 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Termination 

Group 

3F 

10000 mg/m(3) 

Animal 

Number 

3551 3552 3553 3554 3555 

3556 3557 

3558 3559 3560 

Mean 

SD 

n 

ANEU ALYM 

X10A3/UL X10A3/UL 

1.44 10.11 

2.56 7.85 

0.60 5.75 

1. 22 6.01 

2.55 12.56 

1. 84 6.65 1.18 7.63 1.12 6.38 

1.16 6.72 1.78 8.17 

1. 55 7.78 

0.637 2.110 

10 10 

AMONO AEOS ABASO ALUC Poik Poly 

X10A3/UL X10A3/uL X10A3/UL X10A3/uL 

0.10 0.10 0.08 0.05 1+ 1+ 0.49 0.34 0.10 0.06 1+ 1+ 0.07 0.09 0.06 0.04 NAN 1+ 0.26 0.05 0.03 0.03 1+ 1+ 0.26 0. 71 0.14 0.07 NAN 1+ 

0.20 0.34 0.06 0.06 NAN 1+ 0.19 0.12 0.04 0.05 1+ 1+ 0.17 0.09 0.05 0.05 1+ 1+ 0.16 0.25 0.04 0.04 NAN 1+ 0.15 0.13 0.06 0.07 1+ 1+ 0.21 0.22 0.07 0.05 

0.117 0.201 0.033 0.013 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Termination 

Group 

4F 

20000 mg/m(3) 

Animal 

Number 

4561 4562 

4563 

4564 4565 4566 4567 

4568 

4569 

4570 

Mean 

SD 

n 

HGB HCT 

g/dL % 

15.0 44.2 15.8 45.3 16.2 48.5 16.5 48.2 16.3 48.3 16.1 47.9 

cs cs 

15.0 44.2 15.9 46.6 

15.4 45.1 15.8 46.5 

0.55 1. 81 

9 9 

RBC RETIC 

8.04 212.6 8.41 208.4 8.33 232.4 

9.01 147.1 

8.54 196.1 

9.42 127.1 

cs cs 

8.55 194.7 

8.38 216.1 8.83 205.5 8.61 193.3 

0.414 34.14 

9 9 

PLT MCV MCH MCHC 

fL pg g/dL 

1194 54.9 18.7 34.0 

930 53.8 18.8 34.9 

625 58.2 19.5 33.5 

1137 53.5 18.3 34.2 

724 56.5 19.1 33.7 

1076 50.8 17.1 33.7 

cs cs cs cs 

1017 51.7 17.5 33.9 1034 55.6 19.0 34.2 1439 51.1 17.5 34.2 1020 54.0 18.4 34.0 

243.6 2.54 0.84 0.41 

9 9 9 9 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Termination 

Group 

4F 

20000 mg/m(3) 

Animal 

Number 

4561 

4562 4563 4564 

4565 4566 4567 4568 4569 

4570 

Mean 

SD 

n 

ANEU ALYM 

1. 62 7.26 1. 34 8.80 

2.06 7.93 

0.55 5.79 

1. 07 6.69 

0.62 7.80 

cs cs 

1. 38 9.73 1. 22 5.24 

2.16 7.89 

1. 34 7.46 

0.559 1 .. 406 

9 9 

AMONO 

0.11 0.17 0.10 0.06 0.19 0.17 

cs 

0.22 0.20 0.18 

0.16 

0.053 

9 

AEOS ABA SO ALUC Poik Poly 

0.10 0.09 0.05 1+ 1+ 0.12 0.08 0.06 NAN 1+ 0.21 0.00 0.00 NAN 1+ 0.07 0.06 0.02 1+ 1+ 0.19 0.05 0.03 NAN 1+ 

0.12 0.05 0.04 1+ 1+ 

cs cs cs cs cs 

0.25 0.07 0.06 1+ 1+ 0.14 0.00 0.00 NAN 1+ 0.10 0.04 0.08 NAN 1+ 

0.14 0.05 0.04 

0.059 0.032 0.028 

9 9 9 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Recovery 

Group 

1M 

0 mg/m(3) 

Animal 

Number 

1051 1052 1053 1054 

1055 1056 1057 1058 1059 1060 

Mean 

SD 

n 

HGB 

g/dL 

14.8 15.7 15.6 

16.1 

16.0 

15.5 15.9 14.5 16.1 15.2 

15.5 

0.55 

10 

HCT 

% 

46.3 48.4 49.3 49.5 44.0 47.9 48.7 

45.6 

51.7 

49.4 

48.1 

2.23 

10 

RBC 

8.73 

9.06 9.30 9.23 

8.26 8.94 

9.24 

8.79 

9.70 9.72 9.10 

0.446 

10 

RETIC PLT MCV MCH MCHC 

fL pg g/dL 

198.9 1035 53.0 17.0 32.0 139.0 1114 53.5 17.3 32.4 133.1 1232 53.0 16.8 31.7 

210.0 811 53.6 17.4 32.5 

105.5 898 53.2 19.3 36.3 151.8 820 53.5 17.3 32.4 

145.1 867 52.7 17.2 32.6 

186.3 1282 51.9 16.5 31.8 

202.1 686 53.4 16.6 31.2 

152.3 1083 50.8 15.6 30.8 

162.4 983 52.9 17.1 32.4 

34.81 196.2 0.88 0.94 1. 50 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Recovery 

Group 

1M 

o mg/m(3) 

Animal 

Number 

1051 

1052 1053 

1054 1055 1056 1057 1058 1059 1060 

Mean 

SD 

n 

ANEU 

2.11 

1. 02 1. 30 1. 72 1.14 

2.58 

1. 79 1. 79 

2.29 2.08 

1. 78 

0.508 

10 

ALYM 

13.30 11.44 

8.95 

13.44 

7.30 

9.29 

4.54 

10.15 13.02 

7.74 

9.92 

2.938 

10 

AMONO AEOS ABASO ALUC Poik Poly 

0.82 0.20 0.28 0.13 1+ 1+ 0.39 0.17 0.18 0.04 1+ 1+ 0.23 0.06 0.15 0.04 1+ 1+ 0.43 0.14 0.31 0.06 1+ 1+ 0.37 0.12 0.15 0.04 1+ 1+ 0.38 0.33 0.18 0.04 1+ 1+ 0.48 0.07 0.00 0.00 1+ 1+ 

0.69 0.19 0.20 0.08 1+ 1+ 0.49 0.69 0.37 0.05 1+ 1+ 0.32 0.14 0.15 0.05 1+ 1+ 0.46 0.21 0.20 0.05 

0.175 0.185 0.103 0.034 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Recovery 

Group 

4M 

20000 mg/m(3) 

Animal 

Number 

4051 

4052 4053 4054 4055 

4056 4057 4058 4059 

4060 

Mean 

SD 

n 

HGB 

g/dL 

14.9 

15.7 

15.9 15.4 

16.0 

16.1 15.7 

14.5 15.0 

15.6 

15.5 

0.52 

10 

HCT 

% 

45.5 47.9 48.8 48.4 44.9 

49.9 

44.3 

47.1 46.7 48.5 

47.2 

1. 84 

10 

RBC 

X10A6/UL 

9.05 9.30 9.19 9.32 

8.12 

9.13 

8.37 

9.37 

8.99 

9.03 

8.99 

0.416 

10 

RETIC PLT MCV MCH MCHC 

X10A9/L X10A3/UL fL pg g/dL 

128.5 1298 50.3 16.5 32.7 127.0 1308 51.5 16.9 32.7 

131.7 853 53.1 17.3 32.6 

138.2 1159 52.0 16.6 31.9 

133.2 1026 55.2 19.8 35.8 

104.2 1027 54.7 17.6 32.2 124.3 810 52.9 18.8 35.5 127.9 1258 50.3 15.5 30.9 

108.6 599 51.9 16.7 32.1 

144.8 857 53.7 17.3 32.2 126.8 1020 52.6 17.3 32.9 

12.34 238.5 1. 68 1. 22 1. 56 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Recovery 

Group 

4M 

20000 mg/m(3) 

Animal 

Number 

4051 

4052 4053 4054 4055 

4056 4057 

4058 

4059 

4060 Mean 

SD 

n 

ANEU 

X10A3/uL 

1. 24 1.12 

2.20 2.06 

1.41 

2.78 

1. 81 1.41 

3.86 

1. 73 

1. 96 

0.834 

10 

ALYM X10A3/UL 

13.51 11.05 11.76 

6.70 6.26 

10.48 

6.91 

9.03 

3.95 

13.59 

9.32 

3.284 

10 

AMONO AEOS ABASO ALUC Poik Poly 

x10A3/uL X10A3/UL x10A3/uL X10A3/uL 

0.58 0.13 0.19 0.10 1+ 1+ 0.26 0.11 0.16 0.06 1+ 1+ 0.43 0.14 0.22 0.06 1+ 1+ 0.47 0.14 0.20 0.03 1+ 1+ 0.22 0.09 0.08 0.04 1+ 1+ 0.34 0.10 0.19 0.06 NAN 1+ 0.29 0.09 0.10 0.04 NAN 1+ 0.33 0.10 0.21 0.05 1+ 1+ 0.22 0.34 0.05 0.02 1+ 1+ 0.37 0.50 0.19 0.09 1+ 1+ 

0.35 0.17 0.16 0.06 

0.116 0.136 0.060 0.025 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Recovery 

Group 

1F 

0 mg/m(3) 

Animal 

Number 

1551 1552 

1553 1554 1555 1556 1557 1558 

1559 1560 

Mean 

SD 

n 

HGB 

g/dL 

14.2 15.9 16.4 15.3 

15.7 

15.6 14.6 15.0 15.6 14.8 15.3 

0.66 

10 

HCT 

% 

42.0 46.7 

50.1 

46.5 45.9 

46.4 42.7 45.5 47.0 44.9 45.8 

2.27 

10 

RBC RETIC 

X10A6/UL X10A9/L 

7.90 133.9 

8.34 167.4 8.91 132.0 8.25 115.9 8.18 143.5 8.33 114.8 

7.72 157.3 

8.24 136.1 8.28 152.5 8.41 76.5 8.26 133.0 

0.314 26.02 

10 10 

PLT MCV MCH MCHC 

X10A3/uL fL pg g/dL 

1250 53.1 17.9 33.8 

1301 55.9 19.0 34.0 

970 56.3 18.4 32.7 

866 56.3 18.5 32.8 847 56.1 19.2 34.3 

1095 55.7 18.7 33.5 

780 55.3 18.9 34.1 

1223 55.2 18.2 33.0 1017 56.7 18.8 33.2 1092 53.4 17.6 33.1 1044 55.4 18.5 33.5 

180.1 1.22 0.51 0.57 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Recovery 

Group 

1F 

0 mg/m(3) 

Animal 

Number 

1551 1552 1553 1554 1555 1556 1557 1558 1559 

1560 

Mean 

SD 

n 

ANEU 

X10A3/uL 

0.93 

1.15 

1. 60 

2.21 

1. 39 1. 85 

2.63 

1.40 

1.41 

2.50 

1. 71 

0.574 

10 

ALYM 

X10A3/uL 

9.83 9.05 

6.05 

9.40 

8.25 

7.49 7.79 

6.55 

10.06 

8.48 8.30 

1.345 

10 

AMONO AEOS ABA SO ALUC Poik Poly 

x10A3/uL x10A3/uL X10A3/UL X10A3/uL 

0.30 0.07 0.15 0.02 1+ 1+ 0.32 0.08 0.22 0.05 1+ 1+ 0.35 0.15 0.10 0.05 1+ 1+ 0.40 0.14 0.13 0.06 1+ 1+ 0.33 0.08 0.10 0.04 1+ 1+ 0.40 0.13 0.12 0.04 1+ 1+ 0.31 0.16 0.09 0.03 1+ 1+ 0.21 0.09 0.08 0.05 1+ 1+ 

0.20 0.09 0.13 0.05 1+ 1+ 0.59 0.13 0.12 0.03 1+ 1+ 0.34 0.11 0.12 0.04 

0.110 0.033 0.040 0.012 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Recovery 

Group 

4F 

20000 mg/m(3) 

Animal 

Number 

4551 4552 4553 4554 4555 

4556 4557 

4558 

4559 

4560 Mean 

SD 

n 

HGB 

g/dL 

15.7 16.5 15.5 

15.7 15.4 14.8 15.3 15.8 15.3 15.5 

15.6 

0.44 

10 

HCT 

% 

47.0 

50.2 

46.1 47.3 45.4 

43.2 

46.2 47.8 46.4 

49.2 

46.9 

1. 96 

10 

RBC RETIC x10A6/uL X10A9/L 

8.63 99.8 8.91 124.7 

8.65 120.5 8.22 156.8 8.31 139.4 

7.78 99.8 

8.32 82.7 8.91 101.2 8.50 110.0 8.81 150.0 

8.50 118.5 

0.355 24.25 

10 10 

PLT MCV MCH MCHC 

X10A3/UL fL pg g/dL 

1231 54.5 18.2 33.4 

988 56.3 18.5 32.8 931 53.3 17.9 33.6 

1060 57.5 19.1 33.2 1105 54.6 18.5 33.9 

810 55.5 19.0 34.3 

691 55.5 18.5 33.3 

795 53.6 17.7 33.0 

500 54.6 18.0 33.0 

666 55.9 17.6 31.5 

878 55.1 18.3 33.2 

225.7 1. 27 0.51 0.75 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix I 

Individual Hematology Values - Recovery 

Group 

4F 

20000 mg/m(3) 

Animal 

Number 

4551 

4552 4553 4554 

4555 

4556 

4557 

4558 4559 4560 

Mean 

SD 

n 

ANEU 

x10h3/uL 

0.68 

1. 36 1. 06 1.10 1. 25 1. 36 

2.64 

1. 29 

2.82 

1.61 

1.52 

0.685 

10 

ALYM 

x10h3/uL 

7.40 7.54 7.74 

10.19 

7.00 

8.32 8.43 

7.57 

11.55 

7.83 

8.36 

1.425 

10 

AMONO AEOS ABA SO ALUC Poik Poly 

x10h3/uL x10h3/uL x10h3/uL x10h3/uL 

0.20 0.10 0.10 0.03 NAN 1+ 0.22 0.14 0.16 0.03 1+ 1+ 0.20 0.17 0.15 0.02 1+ 1+ 0.19 0.12 0.10 0.04 NAN 1+ 0.14 0.15 0.16 0.03 1+ 1+ 0.20 0.14 0.10 0.06 NAN 1+ 0.26 0.18 0.17 0.04 NAN 1+ 0.30 0.13 0.10 0.02 1+ 1+ 0.28 0.63 0.23 0.06 NAN 1+ 

0.18 0.17 0.31 0.03 NAN 1+ 0.22 0.19 0.16 0.04 

0.049 0.155 0.068 0.014 

10 10 10 10 

P
Huntingdon Life Sciences 00-6130 Page 513

211-DIPE-S Final Report

Individual Coagulation Values

Preface Appendix J

Abbreviation Parameter Reporting Units

PT Prothrombin Time seconds

APTT Activated Partial Thromboplastin Time seconds

Key to Other Abbreviations

NS = No specimen

QNS = Quantity not sufficient to complete request.
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix J 

Individual Coagulation Values - Week 4 

Group 

1M 

0 mg/m(3) 

Animal 

Number 

1061 1062 

1063 

1064 1065 

1066 1067 1068 1069 1070 

Mean 

SD 

n 

PT 

Seconds 

12.4 11.7 12.2 11.4 12.2 10.9 11.5 12.5 12.0 11.7 11.9 

0.50 

10 

APTT 

Seconds 

19.7 

17.2 

18.5 

15.2 

19.9 13.5 14.8 

21.8 

19.6 

16.3 

17.7 

2.67 

10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix J 

Individual Coagulation Values - Week 4 

Group 

2M 

2000 mg/m(3) 

Animal 

Number 

2051 2052 2053 2054 2055 2056 2057 2058 2059 2060 

Mean 

SD 

n 

PT 

Seconds 

13.0 12.5 12.4 

12.7 

12.8 12.2 12.6 11.8 

12.7 12.6 

12.5 

0.34 

10 

APTT 

Seconds 

21.1 

19.5 19.0 

21.1 20.2 

17.9 

21.8 

19.4 

22.9 

21.4 

20.4 

1. 50 

10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix J 

Individual Coagulation Values - Week 4 

Group 

3M 

10000 mg/m(3) 

Animal 

Number 

3051 3052 3053 

3054 3055 3056 

3057 3058 

3059 

3060 

Mean 

SD 

n 

PT 

Seconds 

12.3 12.3 

12.6 

11.9 13.0 11.9 11.9 13.9 12.8 13.0 

12.6 

0.64 

10 

APTT 

Seconds 

18.5 

20.8 21.7 

19.8 

20.2 

18.5 

18.3 

23.1 22.5 20.9 20.4 

1.69 

10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix J 

Individual Coagulation Values - Week 4 

Group 

4M 

20000 mg/m(3) 

Animal 

Number 

4061 

4062 4063 

4064 

4065 4066 4067 

4068 

4069 

4070 

Mean 

SD 

n 

PT 

Seconds 

13.1 

12.3 12.1 12.3 

11.5 12.6 13.5 11.8 11.7 13.6 12.5 

0.74 

10 

APTT 

Seconds 

21.7 20.6 21.0 

19.4 19.0 

23.6 25.3 

18.7 

20.9 21.2 21.1 2.04 

10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix J 

Individual Coagulation Values - Week 4 

Group 

1F 

0 mg/m(3) 

Animal 

Number 

1561 1562 

1563 1564 1565 

1566 

1567 1568 1569 1570 

Mean 

SD 

n 

PT 

Seconds 

11.1 

11.5 11.1 12.3 11.2 11.1 11.2 11.5 

12.0 10.8 

11.4 

0.46 

10 

APTT 

Seconds 

21.4 

20.0 

16.6 

20.2 

19.2 

20.2 

19.4 

20.0 20.5 

20.2 

19.8 

1.26 

10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix J 

Individual Coagulation Values - Week 4 

Group 

2F 2000 mg/m(3) 

Animal 

Number 

2551 2552 2553 2554 2555 2556 2557 2558 2559 2560 

Mean 

SD 

n 

PT 

Seconds 

12.1 11.5 12.2 11.5 

10.3 

12.1 11.4 

QNS 

11.3 

11.5 11.5 

0.58 

9 

APTT 

Seconds 

21.1 

18.8 

21.3 

18.9 17.4 

20.9 

16.5 

QNS 

20.1 

18.2 19.2 1. 71 

9 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix J 

Individual Coagulation Values - Week 4 

Group 

3F 

10000 mg/m(3) 

Animal 

Number 

3551 3552 3553 3554 3555 3556 3557 3558 3559 

3560 

Mean 

SD 

n 

PT 

Seconds 

ll. 5 

11.1 12.0 

ll. 5 

11.7 

ll.4 

12.0 

11.6 

12.0 11.2 

ll.6 

0.33 

10 

APTT 

Seconds 

19.5 18.9 

21.7 20.0 

18.7 18.8 

22.1 

19.8 

22.3 

13.1 

19.5 

2.63 

10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix J 

Individual Coagulation Values - Week 4 

Group 

4F 

20000 mg/m(3) 

Animal 

Number 

4561 

4562 4563 

4564 

4565 

4566 4567 

4568 

4569 

4570 

Mean 

SD 

n 

PT 

Seconds 

12.2 11.1 11.4 11.2 12.1 

12.2 11.5 11.5 

10.9 

11.4 

11.6 

0.46 

10 

APTT 

Seconds 

21.0 

18.7 

19.2 

20.9 21.5 

21.0 

18.7 

19.2 18.9 18.7 19.8 1.16 

10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix J 

Individual Coagulation Values - Termination 

Group 

1M 

0 mg/m(3) 

Animal 

Number 

1061 1062 

1063 1064 1065 

1066 1067 1068 

1069 1070 

Mean 

SD 

n 

PT APTT 

seconds Seconds 

12.2 21.1 

12.2 17.8 

11.9 15.9 

11.8 22.2 

11.8 13.9 

12.0 22.2 

11.8 17.0 

12.6 18.5 

13.2 23.9 12.2 19.2 

12.2 19.2 

0.44 3.16 

10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix J 

Individual Coagulation Values - Termination 

Group 

2M 

2000 mg/m(3) 

Animal 

Number 

2051 2052 2053 2054 2055 2056 2057 2058 2059 

2060 

Mean 

SD 

n 

PT APTT 

Seconds Seconds 

11.8 17.7 12.0 23.4 13.0 22.1 12.8 22.4 12.6 18.2 12.4 20.8 

13.3 24.5 

12.6 19.9 13.6 21.7 

NS NS 

12.7 21.2 

0.58 2.27 

9 9 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix J 

Individual Coagulation Values - Termination 

Group 

3M 

10000 mg/m(3) 

Animal 

Number 

3051 3052 3053 3054 

3055 

3056 

3057 

3058 3059 

3060 

Mean 

SD 

n 

PT APTT 

Seconds Seconds 

12.1 19.1 11.8 17.9 14.0 18.0 

11.6 17.5 

11.5 15.5 12.8 23.0 

12.8 19.2 12.4 20.3 

14.8 25.9 

12.9 21.7 12.7 19.8 1. 06 3.03 

10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix J 

Individual Coagulation Values - Termination 

Group 

4M 

20000 mg/m(3) 

Animal 

Number 

4061 4062 4063 4064 

4065 

4066 4067 4068 

4069 

4070 

Mean 

SD 

n 

PT APTT 

Seconds Seconds 

14.4 20.9 

12.2 18.8 12.0 18.2 

12.7 17.2 

11.8 20.9 

12.6 18.5 12.4 25.9 12.2 20.0 

12.4 23.9 12.3 20.4 

12.5 20.5 

0.72 2.67 

10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix J 

Individual Coagulation Values - Termination 

Group 

1F 

0 mg/m(3) 

Animal 

Number 

1561 1562 

1563 

1564 1565 1566 1567 1568 1569 1570 

Mean 

SD 

n 

PT APTT 

Seconds Seconds 

11.3 20.6 10.6 18.1 

11.2 14.6 11.4 17.8 11.2 19.2 11.5 23.7 

11.1 17.7 11.1 14.2 11.6 20.5 

11.2 15.2 

11.2 18.2 

0.27 2.99 

10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix J 

Individual Coagulation Values - Termination 

Group 

2F 2000 mg/m(3) 

Animal 

Number 

2551 2552 2553 2554 2555 

2556 

2557 2558 2559 2560 

Mean 

SD 

n 

PT APTT 

Seconds Seconds 

11.2 18.1 10.9 17.1 10.7 17.5 

11.2 16.2 10.9 16.4 

11.9 18.0 

10.6 14.8 11.1 15.4 

11.4 14.0 11.1 18.0 

11.1 16.6 

0.37 1. 45 

10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix J 

Individual Coagulation Values - Termination 

Group 

3F 

10000 mg/m(3) 

Animal 

Number 

3551 3552 3553 

3554 3555 

3556 

3557 3558 

3559 

3560 

Mean 

SD 

n 

PT APTT 

Seconds Seconds 

11.4 18.1 11.2 16.0 11.3 20.1 10.7 19.4 

11.6 19.1 

11.1 15.7 

11.0 18.4 11.1 13.9 11.7 17.5 11.2 14.0 

11.2 17.2 

0.29 2.21 

10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix J 

Individual Coagulation Values - Termination 

Group 

4F 

20000 mg/m(3) 

Animal 

Number 

4561 

4562 4563 4564 

4565 4566 4567 4568 4569 

4570 

Mean 

SD 

n 

PT APTT 

Seconds Seconds 

11.5 18.1 

11.2 16.3 

10.5 16.1 10.9 17.6 11.6 19.9 11.4 16.9 11.3 18.6 

11.2 16.5 

10.7 13.6 

11.6 15.3 

11.2 16.9 

0.38 1. 78 

10 10 

P
Huntingdon Life Sciences 00-6130 Page 530

211-DIPE-S Final Report

Individual Clinical Chemistry Values

Preface Appendix K

 Reporting 

Abbreviation Parameter Units 

AST Aspartate Aminotransferase U/L

ALT Alanine Aminotransferase U/L

ALKP Alkaline Phosphatase U/L

LD Lactate Dehydrogenase U/L

SDH Sorbitol Dehydrogenase U/L

BUN Blood Urea Nitrogen mg/dL

CREAT Creatinine mg/dL

GLU Fasting Glucose mg/dL

CK Creatine Kinase U/L

CHOL Cholesterol (Enzymatic) mg/dL

TP Total Protein g/dL

ALB Albumin g/dL

Glob Globulin (calculated) g/dL 

A/G Albumin/Globulin Ratio (calculated)

TBILI Total Bilirubin mg/dL

DBILI Direct Bilirubin mg/dL

IBILI Indirect Bilirubin (calculated) mg/dL

Na+ Sodium mEq/L

K+ Potassium mEq/L

Cl- Chloride mEq/L

Ca++ Calcium mg/dL

PHOS Inorganic Phosphorus mg/dL

GGT Gamma-Glutamyl Transferase U/L

Bile Acids Bile Acids μmol/L

Key to Other Abbreviations

NS = No specimen

QNS = Quantity not sufficient to complete request.
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Group 

1M 

o mg/m(3) 

Animal 

Number 

1061 1062 1063 1064 1065 

1066 1067 1068 1069 

1070 

Mean 

SD 

n 

AST ALT 

U/L U/L 

86 56 81 42 

76 49 

61 34 

77 47 

88 45 83 37 

97 49 

74 41 

85 36 81 44 

9.7 6.9 

10 10 

ALKP 

U/L 

151 140 199 117 

196 134 154 

217 110 

159 158 

35.8 

10 

LD SDH BUN CREAT GLU 

U/L U/L mg/dL mg/dL mg/dL 

217 20.2 14.2 0.4 128 

176 22.6 9.4 0.3 123 

205 22.4 13.8 0.3 111 

172 15.3 11.7 0.3 124 

214 17.3 13.1 0.3 118 160 17.8 12.1 0.3 138 170 18.1 17.2 0.4 135 

178 15.8 14.7 0.3 141 

128 16.4 11.8 0.4 127 

232 14.1 12.9 0.3 127 185 18.0 13.1 0.3 127 

31.4 2.90 2.09 0.05 9.1 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Group 

1M 

0 mg/m(3) 

Animal 

Number 

1061 1062 1063 1064 1065 1066 1067 1068 1069 1070 

Mean 

SD 

n 

CHOL TP 

mg/dL g/dL 

74 6.4 

63 6.7 

64 7.0 

73 6.6 73 6.6 78 6.6 

63 6.2 

52 6.1 52 6.2 

71 6.6 

66 6.5 

9.1 0.27 

10 10 

ALB 

g/dL 

4.2 4.2 4.4 4.3 4.2 4.0 4.0 

3.7 

4.1 4.3 4.1 

0.20 

10 

Glob A/G TBILI DBILI IBILI 

g/dL mg/dL mg/dL mgjdL 

2.2 1.9 0.14 0.04 0.10 

2.5 1.7 0.11 0.03 0.08 

2.6 1.7 0.15 0.05 0.10 

2.3 1.9 0.11 0.04 0.07 

2.4 1.8 0.17 0.04 0.13 

2.6 1.5 0.10 0.03 0.07 

2.2 1.8 0.10 0.03 0.07 2.4 1.5 0.14 0.03 0.11 2.1 2.0 0.13 0.02 0.11 

2.3 1.9 0.10 0.04 0.06 

2.4 1.8 0.13 0.04 0.09 

0.17 0.17 0.025 0.008 0.023 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Animal 

Group Number K+ Cl- Ca++ PHOS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

1M 1061 4.8 102 11.2 9.2 0 96.9 

0 mg/m(3) 1062 5.8 103 11.5 9.7 0 15.6 

1063 5.2 101 11.7 9.8 0 81.1 1064 6.0 102 11.8 9.8 0 17.5 1065 5.6 102 11.4 9.7 0 25.0 

1066 5.4 103 12.0 11.1 0 17.2 

1067 5.8 103 11.4 10.9 0 90.3 

1068 5.8 103 11.4 12.0 2 125.0 

1069 5.4 103 11.4 8.6 0 13.3 1070 6.1 103 11.6 9.6 0 42.7 

Mean 5.6 103 11.5 10.0 0 52.5 

SD 0.40 0.7 0.24 1. 00 0.6 41.76 

n 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Animal 

Group Number AST ALT ALKP LD SDH BUN CREAT GLU 

U/L U/L U/L U/L U/L mg/dL mg/dL mg/dL 

2M 2051 79 36 190 126 13.1 12.5 0.3 118 

2000 mg/m(3) 2052 73 32 141 145 12.1 11.7 0.3 104 

2053 74 38 178 106 14.1 13.4 0.3 119 2054 78 46 142 114 13.4 10.9 0.3 120 2055 71 37 157 113 17.1 14.0 0.3 128 

2056 72 42 147 132 21.9 15.4 0.3 126 2057 74 47 158 141 15.1 14.4 0.3 112 2058 72 32 121 170 15.5 11.0 0.4 126 

2059 78 49 169 171 16.4 10.2 0.3 105 2060 74 35 180 104 14.3 10.4 0.3 125 

Mean 75 39 158 132 15.3 12.4 0.3 118 

SD 2.8 6.2 21.3 24.5 2.77 1. 83 0.03 8.7 

n 10 10 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Animal 

Group Number CHOL TP ALB Glob A/G TBILI DBILI IBILI 

mg/dL g/dL g/dL g/dL mg/dL mg/dL mg/dL 

2M 2051 53 6.5 4.1 2.4 1.7 0.11 0.03 0.08 

2000 mg/m (3) 2052 40 6.2 4.0 2.2 1.8 0.09 0.01 0.08 

2053 61 6.0 4.0 2.0 2.0 0.11 0.03 0.08 2054 59 6.5 4.1 2.4 1.7 0.09 0.02 0.07 2055 61 6.3 4.2 2.1 2.0 0.15 0.03 0.12 2056 55 6.5 4.1 2.4 1.7 0.13 0.04 0.09 2057 78 6.4 4.1 2.3 1.8 0.10 0.03 0.07 2058 77 6.3 4.1 2.2 1.9 0.15 0.04 0.11 2059 67 6.6 4.2 2.4 1.8 0.13 0.04 0.09 2060 60 6.7 4.1 2.6 1.6 0.11 0.03 0.08 

Mean 61 6.4 4.1 2.3 1.8 0.12 0.03 0.09 

SD 11.2 0.21 0.07 0.18 0.13 0.022 0.009 0.016 

n 10 10 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Animal 

Group Number K+ Cl- Ca++ PHOS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

2M 2051 5.6 103 11.8 10.1 0 45.7 

2000 mg/m(3) 2052 5.7 102 11.1 9.3 0 34.6 

2053 5.2 101 11.0 9.7 0 32.2 2054 5.8 101 11.3 9.7 0 11.4 2055 5.0 103 11.3 9.3 0 121.8 2056 5.9 102 11.3 10.3 0 302.8 

2057 5.4 104 11.5 10.4 0 61.6 2058 6.0 101 11.2 10.3 0 26.9 2059 5.3 101 11.5 9.5 0 72.9 2060 5.3 101 11.4 9.8 0 57.1 

Mean 5.5 102 11.3 9.8 0 76.7 

SD 0.33 1.1 0.23 0.41 0.0 85.14 

n 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Animal 

Group Number AST ALT ALKP LD SDH BUN CREAT GLU 

U/L U/L U/L U/L U/L mg/dL mg/dL mg/dL 

3M 3051 89 47 118 141 20.7 11.1 0.3 111 

10000 mg/m(3) 3052 63 34 165 146 12.6 12.2 0.3 121 

3053 105 57 202 272 21.8 11.2 0.4 106 3054 71 31 133 65 16.3 15.4 0.4 122 3055 75 34 156 138 13.5 11.4 0.3 100 

3056 74 37 156 136 13.3 11.1 0.3 100 

3057 76 35 118 76 10.7 8.1 0.2 109 3058 72 39 155 54 13.0 11.0 0.3 112 3059 75 42 186 77 12.1 11.7 0.3 116 3060 84 42 168 73 12.5 12.8 0.3 110 

Mean 78 40 156 118 14.7 11.6 0.3 111 

SD 11.7 7.7 27.2 65.1 3.76 1.81 0.06 7.6 

n 10 10 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Group 

3M 

10000 mg/m(3) 

Animal 

Number 

3051 3052 3053 

3054 

3055 3056 

3057 

3058 3059 3060 

Mean 

SD 

n 

CHOL TP 

mg/dL g/dL 

48 6.6 

69 6.7 

41 6.8 

47 6.5 

49 6.2 

56 6.3 

61 6.1 62 6.6 

71 6.8 

66 5.8 

57 6.4 

10.3 0.33 

10 10 

ALB 

g/dL 

4.1 

4.1 4.4 

4.2 

4.0 4.0 

4.0 

4.3 

4.3 

3.9 

4.1 

0.16 

10 

Glob A/G TBILI DBILI IBILI 

g/dL mg/dL mg/dL mg/dL 

2.5 1.6 0.09 0.02 0.07 

2.6 1.6 0.13 0.03 0.10 2.4 1.8 0.15 0.04 0.11 

2.3 1.8 0.16 0.04 0.12 2.2 1.8 0.09 0.04 0.05 2.3 1.7 0.15 0.04 0.11 

2.1 1.9 0.11 0.03 0.08 2.3 1.9 0.13 0.04 0.09 2.5 1.7 0.11 0.04 0.07 

1.9 2.1 0.13 0.04 0.09 

2.3 1.8 0.13 0.04 0.09 

0.21 0.15 0.025 0.007 0.022 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Animal 

Group Number K+ Cl- Ca++ PHOS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

3M 3051 6.1 105 11.5 10.5 0 13.6 

10000 mg/m(3) 3052 5.3 103 11.6 9.6 0 63.3 

3053 5.6 105 11.5 11.4 0 42.7 3054 5.7 101 11.8 10.6 0 43.1 3055 5.0 103 11.1 9.9 0 221.4 

3056 QNS QNS QNS QNS 0 QNS 3057 5.1 102 11.3 9.8 0 12.6 

3058 5.0 103 11.6 9.3 0 18.1 3059 5.0 102 11.6 9.4 0 19.7 3060 5.4 103 11.2 10.9 0 151.1 

Mean 5.4 103 11.5 10.2 0 65.1 

SD 0.38 1.3 0.22 0.73 0.0 72.88 

n 9 9 9 9 10 9 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Animal 

Group Number AST ALT ALKP LD SDH BUN CREAT GLU 

U/L U/L U/L U/L U/L mg/dL mg/dL mg/dL 

4M 4061 76 37 174 72 18.3 9.1 0.3 118 

20000 mg/m(3) 4062 68 39 119 94 16.9 14.2 0.3 122 

4063 69 36 173 79 12.4 12.9 0.3 107 

4064 89 49 143 98 18.5 11.5 0.3 97 4065 63 43 158 139 11.5 12.0 0.3 113 4066 65 38 154 92 15.1 13.3 0.3 122 4067 57 29 124 77 10.4 13.6 0.3 118 4068 73 34 106 102 13.9 13.1 0.3 114 4069 77 38 134 69 13.0 12.4 0.3 113 4070 71 39 150 72 14.4 11.7 0.4 114 

Mean 71 38 144 89 14.4 12.4 0.3 114 

SD 8.8 5.3 22.7 21.1 2.78 1.44 0.03 7.5 

n 10 10 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Animal 

Group Number CHOL TP ALB Glob A/G TBILI DBILI IBILI 

mg/dL g/dL g/dL g/dL mg/dL mg/dL mg/dL 

4M 4061 46 6.9 4.4 2.5 1.8 0.14 0.03 0.11 

20000 mg/m(3) 4062 53 6.4 3.8 2.6 1.5 0.09 0.03 0.06 

4063 54 6.2 4.0 2.2 1.8 0.11 0.03 0.08 4064 58 6.2 4.2 2.0 2.1 0.11 0.03 0.08 

4065 59 6.0 3.8 2.2 1.7 0.09 0.02 0.07 

4066 69 6.5 4.2 2.3 1.8 0.11 0.04 0.07 4067 67 6.8 4.4 2.4 1.8 0.11 0.03 0.08 4068 77 6.1 3.8 2.3 1.7 0.12 0.02 0.10 4069 49 6.3 4.1 2.2 1.9 0.11 0.05 0.06 

4070 57 6.2 3.9 2.3 1.7 0.08 0.03 0.05 

Mean 59 6.4 4.1 2.3 1.8 0.11 0.03 0.08 

SD 9.6 0.30 0.24 0.17 0.15 0.017 0.009 0.018 

n 10 10 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Animal 

Group Number K+ Cl- Ca++ PHOS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

4M 4061 4.9 101 11.6 9.1 0 10.4 

20000 mg/m(3) 4062 6.0 104 11.3 10.7 0 19.1 

4063 5.5 99 11.5 10.4 0 55.9 4064 5.9 104 11.6 9.5 0 22.5 4065 5.8 105 11.6 9.9 0 28.0 

4066 4.8 102 11.5 9.6 0 33.1 

4067 5.0 102 11.4 8.6 0 33.7 

4068 5.5 105 11.3 10.2 0 75.0 4069 5.3 102 10.8 9.3 0 83.0 

4070 6.3 105 10.7 10.4 0 14.1 

Mean 5.5 103 11.3 9.8 0 37.5 

SD 0.50 2.0 0.33 0.67 0.0 25.35 

n 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Animal 

Group Number AST ALT ALKP LD SDH BUN CREAT GLU 

U/L U/L U/L U/L U/L mg/dL mg/dL mg/dL 

1F 1561 79 48 97 68 16.6 12.7 0.4 126 

o mg/m(3) 1562 77 31 85 68 17.3 13.8 0.6 128 

1563 79 34 106 134 14.6 15.2 0.4 126 1564 93 40 116 93 16.5 16.2 0.5 114 1565 83 38 101 74 15.7 15.9 0.5 119 

1566 104 48 94 174 18.6 15.2 0.5 117 1567 64 29 97 66 16.2 14.4 0.5 124 1568 66 25 105 45 13.2 14.1 0.4 123 1569 57 30 86 73 12.5 14.8 0.4 116 1570 66 41 64 70 11.5 13.4 0.5 123 

Mean 77 36 95 87 15.3 14.6 0.5 122 

SD 14.3 7.9 14.3 38.6 2.26 1.10 0.07 4.8 

n 10 10 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Animal 

Group Number CHOL TP ALB Glob A/G TBILI DBILI IBILI 

mg/dL g/dL g/dL g/dL mg/dL mg/dL mg/dL 

1F 1561 91 7.2 4.8 2.4 2.0 0.13 0.05 0.08 

0 mg/m(3) 1562 95 7.9 5.1 2.8 1.8 0.21 0.06 0.15 

1563 73 6.7 4.2 2.5 1.7 0.19 0.03 0.16 1564 99 6.8 4.4 2.4 1.8 0.21 0.06 0.15 

1565 69 7.4 4.8 2.6 1.8 0.18 0.04 0.14 1566 73 7.8 5.0 2.8 1.8 0.21 0.05 0.16 

1567 75 8.0 5.2 2.8 1.9 0.19 0.04 0.15 1568 96 7.5 4.8 2.7 1.8 0.17 0.04 0.13 

1569 70 6.6 4.4 2.2 2.0 0.15 0.04 0.11 

1570 68 8.3 5.6 2.7 2.1 0.13 0.03 0.10 

Mean 81 7.4 4.8 2.6 1.9 0.18 0.04 0.13 

SD 12.7 0.59 0.42 0.21 0.13 0.031 0. 011 0.028 

n 10 10 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Animal 

Group Number K+ Cl- Ca++ PHOS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

1F 1561 4.8 104 11.7 10.3 0 52.5 

0 mg/m(3) 1562 6.0 100 12.4 10.6 0 92.1 

1563 5.7 101 11.5 10.4 0 141.4 1564 5.5 100 11.7 9.4 0 191.7 1565 5.8 100 12.0 10.3 0 >200. 1566 5.0 102 12.0 9.9 0 104.8 1567 5.2 103 12.2 10.1 0 28.5 1568 4.8 102 12.2 9.9 0 103.6 1569 4.4 103 11.1 9.1 0 26.4 1570 5.6 102 12.6 9.0 0 15.5 

Mean 5.3 102 11.9 9.9 0 84.1 

SD 0.52 1.4 0.45 0.56 0.0 59.01 

n 10 10 10 10 10 9 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Group 

2F 2000 mg/m(3) 

Animal 

Number 

2551 2552 2553 2554 2555 

2556 

2557 2558 2559 2560 

Mean 

SD 

n 

AST ALT 

U/L U/L 

63 28 

74 27 72 40 

86 36 

67 28 

68 30 

97 39 

100 50 

64 26 

88 34 

78 34 

13.8 7.6 

10 10 

ALKP LD U/L U/L 

88 67 

101 72 

70 68 

135 64 

109 108 

122 50 138 219 

101 327 

95 92 

125 99 

108 117 

21.7 88.0 

10 10 

SDH BUN CREAT GLU 

U/L mg/dL mg/dL mg/dL 

10.9 14.3 0.4 115 12.6 10.9 0.3 118 

19.2 12.9 0.4 115 12.3 15.9 0.5 123 

11.9 14.9 0.4 126 

8.7 14.6 0.4 113 

28.0 19.4 0.5 201 

18.5 12.6 0.5 164 12.6 13.5 0.4 149 

21.4 12.9 0.6 125 

15.6 14.2 0.4 135 

5.97 2.31 0.08 28.5 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Animal 

Group Number CHOL TP ALB Glob A/G TBILI DBILI IBILI 

mg/dL g/dL g/dL g/dL mg/dL mg/dL mg/dL 

2F 2551 76 7.4 4.9 2.5 2.0 0.15 0.02 0.13 2000 mg/m(3) 2552 80 7.0 4.5 2.5 1.8 0.15 0.03 0.12 

2553 104 7.9 5.1 2.8 1.8 0.20 0.05 0.15 2554 75 7.5 4.9 2.6 1.9 0.20 0.05 0.15 2555 71 6.8 4.5 2.3 2.0 0.15 0.03 0.12 2556 87 7.1 4.4 2.7 1.6 0.13 0.03 0.10 2557 99 6.7 4.6 2.1 2.2 0.25 0.06 0.19 2558 QNS 7.1 4.4 2.7 1.6 0.12 0.02 0.10 2559 QNS 7.3 4.8 2.5 1.9 0.14 0.03 0.11 2560 87 7.5 5.1 2.4 2.1 0.16 0.03 0.13 

Mean 85 7.2 4.7 2.5 1.9 0.17 0.04 0.13 

SD 11.8 0.36 0.27 0.21 0.20 0.040 0.014 0.027 

n 8 10 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Animal 

Group Number K+ Cl- Ca++ PHOS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

2F 2551 6.3 103 11.9 10.1 0 15.0 2000 mg/m(3) 2552 5.0 104 11.7 9.7 0 20.1 

2553 6.2 101 12.2 8.8 0 19.2 2554 5.3 102 12.2 10.4 1 75.0 2555 5.5 103 11.6 10.3 0 18.4 

2556 5.8 103 12.0 12.0 0 17.6 2557 6.1 99 12.0 15.1 0 >200. 2558 6.6 110 11.6 11.3 0 21.5 2559 6.2 108 12.2 10.4 0 18.2 2560 6.6 107 12.1 10.2 0 33.0 

Mean 6.0 104 12.0 10.8 0 26.4 

SD 0.54 3.4 0.24 1. 73 0.3 18.91 

n 10 10 10 10 10 9 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Group 

3F 

10000 mg/m(3) 

Animal 

Number 

3551 3552 

3553 3554 

3555 3556 

3557 3558 3559 3560 

Mean 

SD 

n 

AST ALT 

U/L U/L 

62 28 

70 37 

71 32 71 35 

75 38 

69 37 63 28 65 35 

69 28 

84 33 

70 33 

6.3 4.0 

10 10 

ALKP 

U/L 

80 

123 

68 

108 

77 92 

80 

78 

119 168 

99 

30.7 

10 

LD SDH BUN CREAT GLU 

U/L U/L mg/dL mg/dL mg/dL 

77 11.2 13.6 0.3 129 

42 10.3 13.2 0.4 114 

65 10.1 11.9 0.4 108 

77 16.0 13.3 0.5 120 

72 10.3 10.3 0.4 119 

47 9.2 14.6 0.4 117 

85 11.7 11.1 0.4 109 

52 10.0 11.6 0.4 114 

66 11.6 11.4 0.5 114 

95 14.6 16.4 0.5 121 

68 11.5 12.7 0.4 117 

16.9 2.17 1. 84 0.06 6.2 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Animal 

Group Number CHOL TP ALB Glob A/G TBILI DBILI IBILI 

mg/dL g/dL g/dL g/dL mg/dL mg/dL mg/dL 

3F 3551 79 7.0 4.7 2.3 2.0 0.14 0.01 0.13 

10000 mg/m(3} 3552 107 7.2 4.5 2.7 1.7 0.20 0.05 0.15 

3553 101 7.6 5.0 2.6 1.9 0.18 0.04 0.14 

3554 94 7.9 5.2 2.7 1.9 0.17 0.04 0.13 3555 83 6.7 4.5 2.2 2.0 0.15 0.03 0.12 3556 QNS 7.5 5.0 2.5 2.0 0.19 0.04 0.15 3557 QNS 7.4 4.8 2.6 1.8 0.14 0.03 0.11 3558 98 7.3 4.7 2.6 1.8 0.13 0.02 0.11 3559 115 7.8 5.0 2.8 1.8 0.24 0.06 0.18 3560 85 7.4 4.7 2.7 1.7 0.17 0.06 0.11 

Mean 95 7.4 4.8 2.6 1.9 0.17 0.04 0.13 

SD 12.5 0.36 0.23 0.19 0.12 0.033 0.016 0.023 

n 8 10 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Animal 

Group Number K+ Cl- Ca++ PHOS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

3F 3551 6.2 108 11.9 11.7 0 16.0 

10000 mg/m(3) 3552 5.3 103 11.9 10.4 0 >200. 

3553 6.0 103 11.8 9.2 0 24.4 

3554 5.8 105 12.0 9.6 0 34.8 3555 5.5 108 11.7 9.6 0 17.1 

3556 5.8 104 12.3 9.8 0 59.7 3557 6.1 105 12.1 10.2 0 15.7 3558 5.9 105 12.3 10.2 0 26.7 3559 5.7 104 12.2 10.0 0 166.4 3560 5.3 105 12.0 11.7 0 >200. 

Mean 5.8 105 12.0 10.2 0 45.1 

SD 0.31 1.8 0.20 0.85 0.0 51.12 

n 10 10 10 10 10 8 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Animal 

Group Number AST ALT ALKP LD SDH BUN CREAT GLU 

U/L U/L U/L U/L U/L mg/dL mg/dL mg/dL 

4F 4561 67 32 74 39 9.0 12.8 0.4 121 

20000 mg/m(3) 4562 71 27 71 60 11.6 15.3 0.4 112 

4563 69 31 68 45 10.1 15.3 0.4 136 

4564 73 30 105 99 16.0 11.2 0.4 106 4565 62 31 100 43 10.3 12.4 0.4 116 4566 64 31 97 37 14.9 14.3 0.4 117 4567 68 32 79 78 11.7 10.4 0.4 122 4568 75 20 98 92 12.7 15.7 0.5 107 4569 70 27 109 77 15.2 13.4 0.4 113 4570 67 30 86 45 8.0 14.9 0.4 114 

Mean 69 29 89 62 12.0 13.6 0.4 116 

SD 3.9 3.7 15.0 23.2 2.73 1. 84 0.03 8.6 

n 10 10 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Group 

4F 

20000 mg/m(3) 

Animal 

Number 

4561 

4562 4563 

4564 4565 

4566 

4567 

4568 

4569 

4570 

Mean 

SD 

n 

CHOL TP 

mg/dL g/dL 

69 6.7 

87 7.4 

69 7.8 

102 7.3 

111 7.0 

90 7.6 

QNS 7.4 

83 6.6 

121 8.2 

QNS 7.0 

92 7.3 

18.8 0.49 

8 10 

ALB 

g/dL 

4.4 4.8 

5.2 

4.6 4.6 

5.0 

4.9 4.4 

5.2 

4.8 

4.8 

0.29 

10 

Glob A/G TBILI DBILI IBILI 

g/dL mg/dL mg/dL mg/dL 

2.3 1.9 0.07 0.03 0.04 2.6 1.8 0.14 0.03 0.11 2.6 2.0 0.18 0.03 0.15 2.7 1.7 0.10 0.02 0.08 2.4 1.9 0.18 0.03 0.15 2.6 1.9 0.16 0.05 0.11 2.5 2.0 0.16 0.02 0.14 2.2 2.0 0.17 0.05 0.12 

3.0 1.7 0.13 0.03 0.10 

2.2 2.2 0.17 0.04 0.13 2.5 1.9 0.15 0.03 0.11 

0.25 0.15 0.037 0.011 0.034 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Week 4 

Animal 

Group Number K+ Cl- Ca++ PHOS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

4F 4561 5.4 106 11.2 10.1 0 17.7 

20000 mg/m(3) 4562 5.9 108 11.6 8.0 0 31.4 

4563 5.5 106 12.4 10.2 0 80.7 4564 6.1 105 11.8 9.9 0 34.8 

4565 5.7 104 11.9 9.4 0 39.1 

4566 5.1 102 11.8 8.7 0 42.7 

4567 5.9 108 11.9 9.3 0 17.5 

4568 5.0 107 11.6 10.2 0 >200. 

4569 6.0 105 12.2 9.6 0 19.7 

4570 5.6 106 11.7 9.7 0 24.5 

Mean 5.6 106 11.8 9.5 0 34.2 

SD 0.37 1.8 0.33 0. 71 0.0 19.76 

n 10 10 10 10 10 9 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Group 

1M 

o mg/m(3) 

Animal 

Number 

1061 1062 1063 1064 1065 1066 1067 1068 1069 1070 

Mean 

SD 

n 

AST 

U/L 

87 

80 86 

79 

79 

90 

78 

114 

84 

73 

85 

11.4 

10 

ALT ALKP 

U/L U/L 

55 88 

43 97 

54 92 

41 117 

43 82 42 69 

34 77 

46 105 46 72 

39 99 

44 90 

6.4 15.2 

10 10 

LD 

U/L 

116 

130 

125 

79 

140 

159 

225 

496 

186 153 

181 

117.6 

10 

SDH BUN CREAT GLU 

U/L mg/dL mg/dL mg/dL 

17.1 12.8 0.4 137 

15.8 13.6 0.4 146 

12.5 13.0 0.5 195 

13.2 15.6 0.4 151 14.1 18.5 0.5 178 

23.3 13.7 0.4 127 

15.0 16.6 0.4 128 

25.8 17.5 0.5 143 

17.7 16.7 0.5 171 

21.2 16.0 0.3 117 

17.6 15.4 0.4 149 

4.48 2.01 0.07 24.9 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Group 

1M 

0 mg/m(3) 

Animal 

Number 

1061 

1062 1063 1064 1065 1066 1067 1068 1069 1070 

Mean 

SD 

n 

CHOL 

mg/dL 

87 

48 

63 

77 

85 82 

63 

59 50 

77 

69 

14.3 

10 

TP ALB 

g/dL g/dL 

7.2 4.3 

7.1 4.3 7.3 4.3 7.1 4.4 7.7 4.6 7.3 4.2 

6.6 4.0 6.5 3.8 

6.9 4.2 

7.1 4.5 7.1 4.3 

0.35 0.23 

10 10 

Glob A/G TBILI DBILI IBILI 

g/dL mg/dL mg/dL mg/dL 

2.9 1.5 0.12 0.05 0.07 2.8 1.5 0.11 0.04 0.07 

3.0 1.4 0.12 0.04 0.08 

2.7 1.6 0.11 0.03 0.08 

3.1 1.5 0.13 0.06 0.07 3.1 1.4 0.10 0.03 0.07 

2.6 1.5 0.10 0.01 0.09 

2.7 1.4 0.10 0.01 0.09 2.7 1.6 0.08 0.01 0.07 2.6 1.7 0.14 0.01 0.13 2.8 1.5 0.11 0.03 0.08 

0.19 0.10 0.017 0.019 0.019 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Animal 

Group Number K+ Cl- Ca++ PROS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

1M 1061 5.7 101 11.7 8.1 0 38.1 

0 mg/m(3) 1062 5.4 103 11.3 8.4 0 24.8 

1063 5.4 103 11.8 10.0 0 68.0 1064 5.6 104 11.6 7.7 0 23.5 1065 6.3 100 12.6 9.2 0 38.7 1066 5.8 99 11.1 8.5 0 13.5 1067 6.2 98 10.5 8.0 0 12.9 1068 5.8 97 10.5 9.2 0 16.3 1069 6.3 96 11.4 8.2 0 13.6 1070 6.3 98 10.8 6.5 0 22.9 

Mean 5.9 100 11.3 8.4 0 27.2 

SD 0.37 2.8 0.65 0.96 0.0 17.16 

n 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Group 

2M 

2000 mg/m(3) 

Animal 

Number 

2051 2052 2053 2054 2055 

2056 

2057 2058 2059 2060 

Mean 

SD 

n 

AST 

U/L 

96 

77 

87 

91 

66 

68 

75 

100 

107 

NS 

85 

14.5 

9 

ALT ALKP 

U/L U/L 

42 67 

39 87 

46 99 

52 115 

37 87 

40 80 

50 99 

39 73 

56 96 

NS NS 

45 89 

6.7 14.8 

9 9 

LD 

U/L 

530 

294 

145 

107 

79 

193 

99 

361 

402 

NS 

246 

159.0 

9 

SDH BUN CREAT GLU 

U/L mg/dL mg/dL mg/dL 

13.3 13.2 0.4 133 11.5 14.7 0.4 103 

20.6 16.7 0.6 205 

13.8 14.4 0.5 235 15.1 15.0 0.4 212 

17.3 16.6 0.4 122 14.2 15.8 0.4 127 

18.5 13.2 0.5 124 

28.6 15.1 0.5 224 

NS NS NS NS 

17.0 15.0 0.5 165 

5.19 1.28 0.07 52.5 

9 9 9 9 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Group 

2M 

2000 mg/m(3) 

Animal 

Number 

2051 2052 2053 2054 2055 

2056 

2057 

2058 

2059 2060 

Mean 

SD 

n 

CHOL TP 

mg/dL g/dL 

67 6.9 

33 6.7 

50 6.6 

63 7.4 

66 6.8 

59 7.1 

85 7.2 

90 7.4 

61 6.8 

NS NS 

64 7.0 

17.0 0.30 

9 9 

ALB 

g/dL 

4.0 4.0 4.3 4.6 4.3 

4.2 4.3 4.4 

4.2 

NS 

4.3 

0.19 

9 

Glob A/G TBILI DBILI IBILI 

g/dL mg/dL mg/dL mg/dL 

2.9 1.4 0.15 0.04 0.11 2.7 1.5 0.08 0.01 0.07 2.3 1.9 0.11 0.03 0.08 2.8 1.6 0.12 0.02 0.10 2.5 1.7 0.11 0.02 0.09 

2.9 1.4 0.08 0.01 0.07 

2.9 1.5 0.12 0.02 0.10 

3.0 1.5 0.15 0.02 0.13 

2.6 1.6 0.11 0.02 0.09 

NS NS 

2.7 1.6 0.11 0.02 0.09 

0.23 0.16 0.025 0.009 0.019 

9 9 9 9 9 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Animal 

Group Number K+ Cl- Ca++ PHOS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

2M 2051 6.0 101 11.3 8.5 0 65.6 

2000 mg/m(3) 2052 5.8 103 10.5 6.6 0 12.1 

2053 4.9 103 10.4 9.9 0 18.6 2054 5.6 101 12.1 8.2 0 16.3 2055 5.1 103 11.9 8.5 0 17.0 2056 6.4 100 10.6 8.0 0 12.9 2057 5.5 97 10.7 7.5 0 16.8 

2058 6.5 98 11.2 8.1 0 22.5 2059 5.6 99 10.8 14.5 0 25.1 2060 NS NS NS NS NS NS 

Mean 5.7 101 11.1 8.9 0 23.0 

SD 0.53 2.2 0.61 2.29 0.0 16.50 

n 9 9 9 9 9 9 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Group 

3M 

10000 mg/m(3} 

Animal 

Number 

3051 

3052 3053 3054 3055 3056 3057 3058 

3059 

3060 

Mean 

SD 

n 

AST 

U/L 

97 

104 

85 

105 

69 

65 

89 

79 

69 

80 84 

14.5 

10 

ALT ALKP 

U/L U/L 

49 82 

33 83 

45 116 

51 70 

41 97 

27 108 

36 82 

41 99 

46 100 43 97 41 93 

7.4 13.9 

10 10 

LD SDH BUN CREAT GLU 

U/L U/L mg/dL mg/dL mg/dL 

281 19.9 11.7 0.4 137 

482 10.9 14.4 0.4 139 

156 23.5 15.1 0.7 178 

123 20.0 14.5 0.5 131 

91 9.8 15.9 0.3 124 

211 15.2 14.4 0.3 106 

278 22.4 11.5 0.5 145 

87 12.2 13.6 0.4 120 

123 13.2 14.7 0.4 114 

62 11.4 14.4 0.4 119 

189 15.9 14.0 0.4 131 

128.5 5.13 1.40 0.12 20.4 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Group 

3M 

10000 mg/m(3) 

Animal 

Number 

3051 3052 3053 3054 3055 

3056 3057 3058 3059 3060 

Mean 

SD 

n 

CHOL TP 

mg/dL g/dL 

67 7.5 

76 7.3 

36 6.8 

51 7.2 51 6.7 

91 7.0 

73 6.6 70 6.9 

71 7.2 

70 6.7 

66 7.0 

15.6 0.30 

10 10 

ALB 

g/dL 

4.4 

4.4 4.3 4.5 

4.2 4.4 4.3 4.4 4.3 

4.3 

4.4 

0.08 

10 

Glob A/G TBILI DBILI IBILI 

g/dL mg/dL mg/dL mg/dL 

3.1 1.4 0.09 0.02 0.07 

2.9 1.5 0.12 0.03 0.09 2.5 1.7 0.11 0.05 0.06 2.7 1.7 0.08 0.03 0.05 2.5 1.7 0.12 0.04 0.08 

2.6 1.7 0.09 0.01 0.08 2.3 1.9 0.11 0.01 0.10 2.5 1.8 0.10 0.02 0.08 2.9 1.5 0.09 0.02 0.07 

2.4 1.8 0.08 0.02 0.06 

2.6 1.7 0.10 0.03 0.07 

0.25 0.16 0.015 0.013 0.015 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry values - Termination 

Animal 

Group Number K+ Cl- Ca++ PHOS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

3M 3051 6.7 104 11.5 8.8 0 32.5 

10000 mg/m(3) 3052 5.5 105 11.2 7.2 0 24.1 

3053 4.9 102 10.8 10.7 0 73.9 3054 6.0 102 11.6 7.0 0 24.9 3055 5.7 102 11.1 7.6 0 23.7 3056 6.2 100 11.2 7.3 0 17.1 3057 5.0 101 10.8 13.6 0 25.4 3058 6.2 101 11.3 8.0 0 17.9 3059 6.4 100 10.6 8.0 0 12.2 3060 5.8 99 11.1 8.1 0 29.2 

Mean 5.8 102 11.1 8.6 0 28.1 

SD 0.58 1.8 0.32 2.04 0.0 17.16 

n 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Group 

4M 

20000 mg/m(3) 

Animal 

Number 

4061 4062 4063 4064 

4065 4066 4067 

4068 4069 4070 

Mean 

SD 

n 

AST ALT 

U/L U/L 

80 41 

75 40 

75 40 

104 59 

68 33 64 30 

56 33 

69 33 

71 40 

62 32 

72 38 

13.1 8.4 

10 10 

ALKP 

U/L 

123 

79 

99 90 79 93 

65 67 

79 

80 

85 

16.9 

10 

LD SDH BUN CREAT GLU 

U/L U/L mg/dL mg/dL mg/dL 

171 25.7 16.2 0.5 185 

133 17.0 13.1 0.4 119 

138 15.3 15.0 0.4 172 

178 22.7 18.1 0.5 133 

51 8.3 13.9 0.4 111 

125 21.4 14.9 0.4 191 

83 10.7 16.1 0.3 131 

64 10.8 12.2 0.3 108 

80 9.6 13.5 0.3 112 

105 12.6 14.7 0.4 97 

113 15.4 14.8 0.4 136 

43.5 6.08 1. 72 0.07 34.2 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Group 

4M 

20000 mg/m(3) 

Animal 

Number 

4061 

4062 4063 4064 4065 

4066 

4067 

4068 

4069 

4070 

Mean 

SD 

n 

CHOL 

mg/dL 

34 

42 

61 

58 57 

80 

86 

84 

55 54 

61 

17.4 

10 

TP ALB 

g/dL g/dL 

6.7 4.3 

7.1 4.4 

6.8 4.1 6.7 4.3 6.2 3.9 

7.3 4.4 

7.6 4.5 

6.6 4.0 6.8 4.3 6.6 4.0 

6.8 4.2 

0.40 0.20 

10 10 

Glob A/G TBILI DBILI IBILI 

g/dL mg/dL mg/dL mg/dL 

2.4 1.8 0.11 0.04 0.07 2.7 1.6 0.09 0.02 0.07 2.7 1.5 0.08 0.02 0.06 2.4 1.8 0.09 0.03 0.06 2.3 1.7 0.07 0.03 0.04 2.9 1.5 0.12 0.02 0.10 

3.1 1.5 0.09 0.02 0.07 

2.6 1.5 0.07 0.01 0.06 

2.5 1.7 0.13 0.03 0.10 2.6 1.5 0.08 0.01 0.07 

2.6 1.6 0.09 0.02 0.07 

0.24 0.13 0.021 0.009 0.018 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Animal 

Group Number K+ Cl- Ca++ PROS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

4M 4061 6.7 100 11.1 9.6 0 30.0 

20000 mg/m(3) 4062 6.3 105 11.1 8.6 0 21.9 

4063 5.8 103 10.9 10.3 0 14.8 4064 5.1 107 11.2 12.1 0 35.0 

4065 5.9 103 11.0 8.0 0 24.6 4066 5.0 101 10.5 8.1 0 25.0 4067 5.7 98 10.9 7.5 0 13.5 

4068 5.7 100 10.8 7.2 0 13.9 4069 5.6 98 10.7 7.5 2 24.9 4070 6.7 100 10.9 8.8 0 10.9 

Mean 5.9 102 10.9 8.8 0 21.5 

SD 0.58 3.0 0.21 1. 52 0.6 7.94 

n 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Group 

1F 

0 mg/m(3) 

Animal 

Number 

1561 1562 1563 1564 1565 1566 1567 1568 1569 1570 

Mean 

SD 

n 

AST 

U/L 

82 

90 

104 

91 78 

134 

70 

104 

80 80 

91 

18.6 

10 

ALT ALKP 

U/L U/L 

45 60 

38 27 

38 47 32 48 

35 43 

63 42 

26 36 

38 42 

48 50 

52 30 

42 43 

10.7 9.7 

10 10 

LD SDH BUN CREAT GLU 

U/L U/L mg/dL mg/dL mg/dL 

247 18.5 15.2 0.4 111 

343 17.3 12.0 0.5 114 

434 14.6 16.0 0.5 124 

299 16.9 14.2 0.6 164 

99 12.8 14.5 0.5 127 

225 25.0 17.2 0.5 135 

281 15.7 16.1 0.4 142 

129 27.4 17.0 0.5 195 

205 18.8 16.0 0.5 160 

439 14.5 12.2 0.5 131 

270 18.2 15.0 0.5 140 

114.3 4.67 1. 82 0.06 25.9 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Group 

1F 

o mg/m(3) 

Animal 

Number 

1561 1562 

1563 

1564 1565 1566 1567 1568 1569 

1570 

Mean 

SD 

n 

CHOL 

mg/dL 

71 

82 

66 

87 

63 64 

57 

85 

95 96 

77 

14.1 

10 

TP ALB 

g/dL g/dL 

7.5 4.8 

8.1 5.2 

7.3 4.5 

6.7 4.5 

7.5 5.0 

7.7 5.0 

8.0 5.4 

7.9 5.1 7.3 5.1 

9.4 6.2 

7.7 5.1 

0. 71 0.49 

10 10 

Glob A/G TBILI DBILI IBILI 

g/dL mg/dL mg/dL mg/dL 

2.7 1.8 0.13 0.04 0.09 

2.9 1.8 0.19 0.05 0.14 2.8 1.6 0.13 0.02 0.11 2.2 2.0 0.10 0.03 0.07 2.5 2.0 0.12 0.01 0.11 2.7 1.9 0.16 0.04 0.12 2.6 2.1 0.13 0.03 0.10 2.8 1.8 0.12 0.02 0.10 2.2 2.3 0.21 0.04 0.17 

3.2 1.9 0.11 0.01 0.10 

2.7 1.9 0.14 0.03 0.11 

0.31 0.19 0.036 0.014 0.028 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Animal 

Group Number K+ Cl- Ca++ PHOS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

1F 1561 5.2 104 11.1 6.3 0 31.8 

0 mg/m(3) 1562 4.7 103 11.7 5.8 0 25.8 

1563 4.9 108 10.9 7.2 0 34.5 1564 4.6 104 10.8 6.9 0 13.9 

1565 6.1 106 11.4 6.2 0 11.8 1566 4.6 103 11.2 9.8 0 62.9 1567 4.4 100 11.0 6.9 0 18.0 1568 3.7 100 11.1 6.1 0 94.5 1569 4.4 100 11.6 8.4 0 63.4 

1570 5.1 97 12.0 4.8 0 12.0 

Mean 4.8 103 11.3 6.8 0 36.9 

SD 0.63 3.3 0.39 1.41 0.0 27.86 

n 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Group 

2F 2000 mg/m(3) 

Animal 

Number 

2551 2552 2553 2554 

2555 

2556 

2557 

2558 2559 2560 

Mean 

SD 

n 

AST ALT 

U/L U/L 

89 33 

88 29 81 47 81 30 81 27 

72 29 

80 34 

108 63 

80 31 

78 35 

84 36 

9.8 11.1 

10 10 

ALKP 

U/L 

41 44 

33 

74 

50 

53 

39 

55 

46 47 48 

11.2 

10 

LD SDH BUN CREAT GLU 

U/L U/L mg/dL mg/dL mg/dL 

669 10.8 15.4 0.5 121 

379 14.6 15.7 0.4 120 

183 17.7 17.8 0.6 113 

263 14.5 15.4 0.5 120 

441 14.0 18.7 0.5 140 

158 15.3 19.3 0.5 131 

270 13.6 13.6 0.4 113 

387 15.9 18.2 0.6 123 319 19.4 16.7 0.5 167 

106 14.8 14.7 0.6 197 

318 15.1 16.6 0.5 135 

163.6 2.32 1. 89 0.07 27.2 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Group 

2F 2000 mg/m (3) 

Animal 

Number 

2551 2552 

2553 2554 2555 2556 2557 

2558 2559 2560 

Mean 

SD 

n 

CHOL TP 

mg/dL g/dL 

65 7.7 

85 7.4 85 8.1 

65 7.5 69 7.0 66 7.1 

126 8.6 

57 7.6 

90 7.8 

90 7.8 

80 7.7 

20.2 0.47 

10 10 

ALB 

g/dL 

5.1 

4.8 

5.5 

5.1 

4.8 4.5 

6.0 

4.9 

5.0 5.5 5.1 

0.44 

10 

Glob A/G TBILI DBILI IBILI 

g/dL mg/dL mg/dL mg/dL 

2.6 2.0 0.20 0.05 0.15 2.6 1.8 0.11 0.02 0.09 2.6 2.1 0.14 0.05 0.09 2.4 2.1 0.14 0.04 0.10 2.2 2.2 0.17 0.04 0.13 

2.6 1.7 0.11 0.02 0.09 2.6 2.3 0.22 0.04 0.18 2.7 1.8 0.11 0.02 0.09 2.8 1.8 0.12 0.02 0.10 

2.3 2.4 0.16 0.03 0.13 

2.5 2.0 0.15 0.03 0.12 

0.18 0.24 0.039 0.013 0.031 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Animal 

Group Number K+ Cl- Ca++ PHOS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

2F 2551 5.7 106 11.7 6.6 0 42.2 2000 mg/m(3) 2552 4.9 106 11.5 6.5 0 13.2 

2553 5.9 104 12.3 6.0 0 18.2 2554 5.7 106 11.4 6.3 0 16.8 2555 5.2 105 11.5 8.2 0 36.1 2556 6.2 99 10.6 6.1 0 15.7 2557 5.1 98 11.5 5.9 0 81.5 2558 6.3 100 11.0 6.4 0 9.3 2559 6.2 106 11.8 8.4 0 17.7 2560 5.7 99 12.2 7.3 0 78.6 

Mean 5.7 103 11.6 6.8 0 32.9 

SD 0.49 3.4 0.51 0.90 0.0 26.86 

n 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Group 

3F 

10000 mg/m(3) 

Animal 

Number 

3551 3552 3553 3554 3555 3556 3557 3558 3559 3560 

Mean 

SD 

n 

AST 

U/L 

83 

63 67 61 

78 

74 

64 

76 

58 

85 

71 

9.6 

10 

ALT ALKP 

U/L U/L 

36 57 

31 29 

33 31 

30 34 

37 39 

34 40 

35 39 

37 38 

25 52 

35 67 

33 43 

3.7 12.2 

10 10 

LD SDH BUN CREAT GLU 

U/L U/L mg/dL mg/dL mg/dL 

225 21.9 16.1 0.5 122 

84 11.4 13.7 0.4 130 

98 12.4 15.4 0.5 113 

115 12.2 15.9 0.5 112 150 13.6 16.5 0.5 138 111 14.7 12.1 0.5 134 

122 13.5 12.8 0.4 140 

110 13.6 15.1 0.5 142 

92 20.5 13.6 0.6 176 

123 16.7 13.3 0.5 152 123 15.1 14.5 0.5 136 

40.3 3.57 1. 53 0.06 19.0 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Group 

3F 

10000 mg/m(3) 

Animal 

Number 

3551 3552 3553 3554 3555 3556 

3557 3558 3559 3560 

Mean 

SD 

n 

CHOL 

mg/dL 

88 

62 

79 

127 

88 

80 

106 

79 

96 

92 90 

17.6 

10 

TP ALB 

g/dL g/dL 

7.5 4.7 

6.8 4.9 

8.0 5.4 

9.1 6.0 

6.9 4.2 

7.9 5.1 

8.0 5.2 

7.5 4.9 7.7 5.1 7.6 5.0 7.7 5.1 

0.64 0.46 

10 10 

Glob A/G TBILI DBILI IBILI 

g/dL mg/dL mg/dL mg/dL 

2.8 1.7 0.14 0.02 0.12 

1.9 2.6 0.14 0.03 0.11 

2.6 2.1 0.14 0.04 0.10 

3.1 1.9 0.15 0.04 0.11 

2.7 1.6 0.12 0.02 0.10 2.8 1.8 0.14 0.03 0.11 2.8 1.9 0.12 0.01 0.11 2.6 1.9 0.12 0.01 0.11 2.6 2.0 0.14 0.02 0.12 2.6 1.9 0.11 0.02 0.09 2.7 1.9 0.13 0.02 0.11 

0.31 0.27 0.013 0.011 0.009 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Animal 

Group Number K+ Cl- Ca++ PHOS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

3F 3551 5.3 107 11.7 8.8 0 46.1 

10000 mg/m(3) 3552 5.5 103 11.4 6.6 0 13.6 

3553 6.3 105 11.6 6.3 0 14.8 

3554 4.8 99 12.2 6.4 0 53.6 

3555 5.8 105 11.3 6.3 0 22.4 3556 5.9 101 11.6 6.5 0 13.4 3557 5.1 101 11.4 5.8 0 12.1 3558 6.0 101 11.4 6.7 0 21.1 3559 5.2 104 11.6 9.6 0 30.8 3560 5.3 103 11.4 8.6 0 19.3 

Mean 5.5 103 11.6 7.2 0 24.7 

SD 0.47 2.4 0.26 1. 32 0.0 14.47 

n 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Group 

4F 

20000 mg/m(3) 

Animal 

Number 

4561 4562 

4563 

4564 4565 4566 4567 4568 4569 

4570 

Mean 

SD 

n 

AST 

U/L 

88 

69 

96 

74 

65 

87 

69 

70 

58 

70 

75 

11.9 

10 

ALT ALKP U/L U/L 

41 34 

29 so 

72 45 

39 43 

29 49 

36 37 

31 44 

31 38 

25 46 

31 52 

36 44 

13.4 5.9 

10 10 

LD 

U/L 

153 

83 

106 

120 

66 

74 

66 67 

81 

48 

86 

31.3 

10 

SDH BUN CREAT GLU 

U/L mg/dL mg/dL mg/dL 

16.7 13.5 0.5 113 11.4 16.6 0.5 106 

21.9 15.3 0.5 167 

12.8 14.1 0.5 145 11.1 15.7 0.5 119 13.2 16.5 0.4 135 11.1 19.6 0.5 128 10.7 15.5 0.5 128 10.8 12.7 0.5 129 

7.0 16.7 0.5 121 

12.7 15.6 0.5 129 

4.05 1.95 0.03 17.3 

10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry values - Termination 

Group 

4F 

20000 mg/m(3) 

Animal 

Number 

4561 

4562 4563 4564 4565 

4566 

4567 

4568 4569 

4570 

Mean 

SD 

n 

CHOL TP 

mg/dL g/dL 

67 7.5 

93 7.7 

75 8.4 

85 8.0 

97 7.6 

94 8.3 

101 8.4 

98 7.4 

112 8.5 

86 7.4 

91 7.9 

13.0 0.45 

10 10 

ALB 

g/dL 

4.9 

5.0 5.3 5.0 

4.9 

5.5 5.7 

4.5 

5.5 

4.7 

5.1 

0.39 

10 

Glob A/G TBILI DBILI IBILI 

g/dL mg/dL mg/dL mg/dL 

2.6 1.9 0.13 0.02 0.11 2.7 1.9 0.12 0.04 0.08 

3.1 1.7 0.14 0.04 0.10 3.0 1.7 0.09 0.03 0.06 

2.7 1.8 0.15 0.04 0.11 

2.8 2.0 0.13 0.03 0.10 2.7 2.1 0.13 0.03 0.10 2.9 1.6 0.12 0.02 0.10 

3.0 1.8 0.17 0.03 0.14 

2.7 1.7 0.15 0.04 0.11 

2.8 1.8 0.13 0.03 0.10 

0.17 0.15 0.022 0.008 0.021 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Termination 

Animal 

Group Number K+ Cl- Ca++ PROS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

4F 4561 6.8 104 11.5 7.2 1 13.9 

20000 mg/m(3) 4562 5.8 103 11.8 6.5 0 17.9 

4563 5.5 106 13.0 8.3 0 127.6 4564 6.7 109 12.2 7.6 0 18.5 

4565 5.6 107 11.9 6.4 0 16.4 4566 5.9 100 11.9 8.0 0 80.3 4567 5.5 97 11.9 7.5 0 165.6 

4568 6.1 103 11.5 7.6 0 53.7 

4569 6.0 100 11.7 5.2 1 14.4 

4570 6.5 101 11.4 6.8 0 32.1 

Mean 6.0 103 11.9 7.1 0 54.0 

SD 0.48 3.7 0.46 0.91 0.4 53.95 

n 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Recovery 

Animal 

Group Number AST ALT ALKP LD SDH BUN CREAT GLU 

U/L U/L U/L U/L U/L mg/dL mg/dL mg/dL 

1M 1051 86 44 96 114 18.7 11.4 0.3 107 

0 mg/m(3) 1052 34 44 82 686 9.8 13.0 0.4 121 

1053 68 48 89 92 17.0 11.8 0.3 98 1054 93 44 79 270 19.9 13.7 0.4 99 1055 73 42 100 153 16.8 11.3 0.3 99 

1056 92 42 62 126 19.3 13.6 0.4 104 1057 193 115 79 193 29.9 13.6 0.4 148 1058 70 40 102 112 18.1 11.9 0.3 113 1059 92 41 73 190 25.9 13.3 0.4 193 

1060 88 51 67 158 20.3 13.0 0.4 98 

Mean 89 51 83 209 19.6 12.7 0.4 118 

SD 40.7 22.7 13.7 175.3 5.38 0. 96 0.05 30.5 

n 10 10 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Recovery 

Animal 

Group Number CHOL TP ALB Glob A/G TBILI DBILI IBILI 

mg/dL g/dL g/dL g/dL mg/dL mg/dL mg/dL 

1M 1051 91 7.0 4.0 3.0 1.3 0.13 0.03 0.10 

o mg/m{3) 1052 62 7.0 4.2 2.8 1.5 0.13 0.03 0.10 

1053 75 6.8 4.0 2.8 1.4 0.13 0.04 0.09 1054 78 7.4 4.6 2.8 1.6 0.17 0.06 0.11 1055 61 6.8 4.2 2.6 1.6 0.11 0.04 0.07 1056 81 7.3 4.2 3.1 1.4 0.12 0.02 0.10 1057 56 6.7 4.1 2.6 1.6 0.11 0.03 0.08 1058 58 7.2 4.1 3.1 1.3 0.11 0.04 0.07 1059 57 6.9 4.3 2.6 1.7 0.15 0.03 0.12 1060 51 6.9 4.2 2.7 1.6 0.12 0.04 0.08 

Mean 67 7.0 4.2 2.8 1.5 0.13 0.04 0.09 

SD 13.2 0.23 0.17 0.20 0.14 0.019 0. 011 0.017 

n 10 10 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Recovery 

Animal 

Group Number K+ Cl- Ca++ PROS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

1M 1051 6.9 103 11.4 8.8 0 33.5 

o mg/m{3) 1052 6.6 101 11.0 7.7 0 16.6 

1053 6.1 102 11.6 7.3 0 66.5 1054 5.7 103 11.5 7.3 1 29.5 

1055 5.8 103 11.0 7.3 0 12.9 

1056 6.6 104 11.2 8.1 0 34.5 1057 5.2 105 10.8 7.9 1 74.0 

1058 6.4 103 11.0 6.7 0 13.7 1059 5.9 106 11.6 8.6 0 75.6 1060 6.2 105 11.2 7.9 0 36.3 

Mean 6.1 104 11.2 7.8 0 39.3 

SD 0.51 1.5 0.28 0.64 0.4 24.25 

n 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Recovery 

Group 

4M 

20000 mg/m(3) 

Animal 

Number 

4051 

4052 4053 

4054 

4055 

4056 4057 4058 4059 

4060 

Mean 

SD 

n 

AST ALT 

U/L U/L 

86 56 

68 38 

70 39 

68 43 62 29 

67 39 

81 44 

65 60 

86 47 

63 38 

72 43 

9.2 9.1 

10 10 

ALKP 

U/L 

90 91 

88 82 84 83 

77 

80 

91 98 

86 

6.3 

10 

LD SDH BUN CREAT GLU 

U/L U/L mg/dL mg/dL mg/dL 

169 18.5 13.1 0.4 185 

92 14.1 9.8 0.3 101 

73 14.0 12.9 0.3 102 

97 15.6 12.9 0.4 105 

133 14.3 13.8 0.4 85 

73 10.3 14.4 0.3 94 

59 12.1 12.5 0.4 118 

69 18.9 14.0 0.3 116 68 15.8 10.6 0.3 102 

109 13.5 12.0 0.3 119 

94 14.7 12.6 0.3 113 

34.6 2.64 1.46 0.05 27.6 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Recovery 

Animal 

Group Number CHOL TP ALB Glob A/G TBILI DBILI I BILl 

mg/dL g/dL g/dL g/dL mg/dL mg/dL mg/dL 

4M 4051 84 6.9 4.1 2.8 1.5 0.13 0.01 0.12 

20000 mg/m(3) 4052 72 6.9 4.3 2.6 1.7 0.15 0.04 0.11 

4053 62 7.0 4.2 2.8 1.5 0.13 0.03 0.10 4054 80 6.8 4.0 2.8 1.4 0.12 0.02 0.10 4055 58 6.8 4.2 2.6 1.6 0.13 0.05 0.08 4056 53 7.1 4.4 2.7 1.6 0.13 0.04 0.09 

4057 38 6.8 4.4 2.4 1.8 0.11 0.04 0.07 4058 96 7.0 4.2 2.8 1.5 0.14 0.04 0.10 4059 43 6.5 4.2 2.3 1.8 0.14 0.03 0.11 

4060 81 6.8 4.0 2.8 1.4 0.11 0.02 0.09 Mean 67 6.9 4.2 2.7 1.6 0.13 0.03 0.10 

SD 19.0 0.16 0.14 0.18 0.15 0.013 0.012 0.015 

n 10 10 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Recovery 

Animal 

Group Number K+ Cl- Ca++ PHOS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

4M 4051 5.6 105 11.8 8.1 0 31.1 

20000 mg/m(3} 4052 6.3 104 11.0 7.2 1 43.3 

4053 6.0 105 11.4 7.4 0 25.3 4054 6.4 106 10.4 6.6 0 42.9 

4055 6.2 106 10.8 7.6 0 37.3 

4056 6.1 104 11.3 7.2 0 40.1 4057 6.2 107 10.7 7.3 0 11.8 4058 6.4 103 11.5 7.5 0 22.1 4059 6.3 106 10.9 7.7 0 38.2 

4060 6.0 107 11.4 7.6 0 17.3 

Mean 6.2 105 11.1 7.4 0 30.9 

SD 0.24 1.3 0.43 0.39 0.3 11.23 

n 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Recovery 

Animal 

Group Number AST ALT ALKP LD SDH BUN CREAT GLU 

U/L U/L U/L U/L U/L mg/dL mg/dL mg/dL 

1F 1551 111 76 20 95 23.9 14.1 0.5 98 

0 mg/m(3) 1552 97 63 66 140 19.1 15.5 0.4 107 

1553 97 57 36 246 20.1 15.6 0.5 95 1554 72 36 48 229 16.1 14.8 0.4 113 

1555 70 49 44 136 18.0 10.8 0.4 102 1556 86 40 46 120 16.6 11.4 0.5 95 1557 77 31 36 85 11.9 16.2 0.4 88 1558 321 175 44 192 40.6 15.1 0.4 105 1559 77 43 34 115 14.1 13.5 0.4 97 1560 80 46 40 136 13.8 13.8 0.4 94 

Mean 109 62 41 149 19.4 14.1 0.4 99 

SD 75.7 42.0 11.8 54.9 8.21 1. 79 0.05 7.4 

n 10 10 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Recovery 

Animal 

Group Number CHOL TP ALB Glob A/G TBILI DBILI IBILI 

mg/dL g/dL g/dL g/dL mg/dL mg/dL mg/dL 

1F 1551 145 9.0 6.0 3.0 2.0 0.20 0.05 0.15 

0 mg/m(3) 1552 94 7.2 4.9 2.3 2.1 0.18 0.05 0.13 

1553 75 7.6 4.8 2.8 1.7 0.16 0.03 0.13 1554 89 7.4 4.7 2.7 1.7 0.19 0.04 0.15 1555 93 7.6 5.2 2.4 2.2 0.18 0.03 0.15 1556 78 7.4 4.9 2.5 2.0 0.17 0.04 0.13 1557 104 8.3 5.5 2.8 2.0 0.18 0.07 0.11 

1558 81 7.3 4.8 2.5 1.9 0.22 0.06 0.16 1559 94 7.0 4.8 2.2 2.2 0.14 0.03 0.11 1560 107 7.0 4.3 2.7 1.6 0.14 0.03 0.11 

Mean 96 7.6 5.0 2.6 1.9 0.18 0.04 0.13 

SD 20.1 0.62 0.47 0.25 0.21 0.025 0.014 0.019 

n 10 10 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Recovery 

Animal 

Group Number K+ Cl- Ca++ PHOS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

1F 1551 5.3 101 12.5 5.6 0 63.7 

0 mg/m{3) 1552 5.7 108 11.4 7.1 0 19.6 

1553 6.1 105 11.5 6.1 0 14.5 1554 5.4 103 11.3 7.0 0 61.4 1555 5.6 107 11.6 6.1 0 18.0 1556 5.9 109 11.4 6.6 0 13.4 1557 5.1 103 12.0 5.5 0 52.4 1558 6.0 108 11.5 6.9 2 57.6 1559 5.9 109 11.3 6.9 1 16.6 1560 6.0 108 11.0 6.9 0 24.4 

Mean 5.7 106 11.6 6.5 0 34.2 

SD 0.34 2.9 0.42 0.60 0.7 21.58 

n 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Recovery 

Animal 

Group Number AST ALT ALKP LD SDH BUN CREAT GLU 

U/L U/L U/L U/L U/L mg/dL mg/dL mg/dL 

4F 4551 51 29 28 50 8.0 12.7 0.4 107 

20000 mg/m(3) 4552 60 38 26 87 10.6 13.2 0.4 95 

4553 70 40 33 52 9.7 15.4 0.5 103 4554 54 33 51 54 9.2 14.1 0.5 109 

4555 88 70 32 78 20.0 17.4 0.4 100 4556 99 50 50 97 15.4 17.8 0.5 85 4557 97 69 39 58 13.6 15.5 0.5 92 

4558 60 35 40 54 9.9 14.0 0.5 105 

4559 77 33 57 57 8.6 19.1 0.4 107 

4560 149 113 43 101 23.4 12.3 0.5 125 Mean 81 51 40 69 12.8 15.2 0.5 103 

SD 29.6 26.3 10.4 19.9 5.25 2.31 0.05 10.9 

n 10 10 10 10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Recovery 

Group 

4F 

20000 mg/m(3) 

Animal 

Number 

4551 4552 

4553 

4554 4555 4556 4557 4558 

4559 

4560 

Mean 

SD 

n 

CHOL TP 

mg/dL g/dL 

74 7.8 

95 8.0 

96 7.7 94 8.1 

83 8.5 

105 7.1 

79 8.1 70 7.6 

53 7.5 

115 8.5 

86 7.9 

18.2 0.44 

10 10 

ALB 

g/dL 

5.4 5.5 

5.0 5.5 5.7 

4.6 4.5 

4.8 4.8 

5.6 5.1 

0.45 

10 

Glob A/G TBILI DBILI IBILI 

g/dL mg/dL mg/dL mg/dL 

2.4 2.3 0.18 0.05 0.13 2.5 2.2 0.19 0.05 0.14 

2.7 1.9 0.16 0.03 0.13 2.6 2.1 0.18 0.05 0.13 2.8 2.0 0.14 0.02 0.12 

2.5 1.8 0.16 0.03 0.13 

3.6 1.3 0.16 0.03 0.13 

2.8 1.7 0.08 0.02 0.06 2.7 1.8 0.24 0.08 0.16 

2.9 1.9 0.17 0.04 0.13 

2.8 1.9 0.17 0.04 0.13 

0.34 0.28 0.040 0.018 0.025 

10 10 10 10 10 

P
Huntingdon Life Sciences Study No.: 00-6130 

Sponsor Study No.: 211-DIPE-S 

Appendix K 

Individual Clinical Chemistry Values - Recovery 

Animal 

Group Number K+ Cl- Ca++ PHOS GGT Bile 

Acids 

mEq/L mEq/L mg/dL mg/dL U/L umol/L 

4F 4551 6.1 106 12.3 6.5 0 30.6 

20000 mg/m(3) 4552 5.7 105 11.6 6.4 0 67.7 

4553 5.6 106 11.4 4.9 0 31.0 4554 5.5 106 11.6 5.6 0 60.5 

4555 5.2 105 11.7 5.4 0 43.1 4556 5.9 110 11.2 6.8 2 19.5 

4557 5.0 108 12.0 6.6 0 17.7 

4558 5.3 105 12.0 6.1 1 27.7 4559 5.5 108 11.7 7.3 0 210.8 

4560 5.0 106 12.7 6.4 0 31.7 

Mean 5.5 107 11.8 6.2 0 54.0 

SD 0.36 1.6 0.44 0. 71 0.7 57.45 

n 10 10 10 10 10 10 

P
Huntingdon Life Sciences 00-6130 Page 591

211-DIPE-S Final Report

Individual Organ Weights and

Brain Measurements

Preface Appendix L

Absolute Organ Weights (Terminal Sacrifice)................................................... 592

% Organ to Body Weight (Terminal Sacrifice) .................................................. 600

% Organ to Brain Weight (Terminal Sacrifice).................................................. 608

Brain Measurements (Terminal Sacrifice) ....................................................... 616

Absolute Organ Weights (Recovery Sacrifice).................................................. 618

% Organ to Body Weight (Recovery Sacrifice)................................................. 622

% Organ to Brain Weight (Recovery Sacrifice)................................................. 626

Note: Brain length and width were measured in centimeters.

Corresponding exposure levels for each group were as follows:

Group I - 0 mg/m3

Group II - 2000 mg/m3

Group III - 10000 mg/m3

Group IV - 20000 mg/m3

Key to Abbreviations

g = Grams

wt. = Weight

observ. = Observations
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal 

Summary statistics for absolute organ weights (g) 

Study number: 006130 

Brain Heart 

No/sex Subgroup Body wt. (g) Adrenal Glands Epididymis 

1061/M 1/1 1062/M 1/1 1063/M 1/1 1064/M 1/1 1065/M 1/1 1066/M 1/1 1067/M 1/1 1068/M 1/1 1069/M 1/1 

1070/M 1/1 

Me an: 

Standard deviation: 

Number of observ. 

2051/M 2052/M 2053/M 2054/M 2055/M 2056/M 2057/M 2058/M 

2059/M 

2/1 2/1 

2/1 

2/1 

2/1 

2/1 2/1 2/1 

2/1 

M e a n: 

Standard deviation: 

Number of observ. 

3051/M 3052/M 3053/M 3054/M 

3055/M 3056/M 

3057/M 3058/M 3059/M 3060/M 

3/1 3/1 3/1 3/1 3/1 3/1 3/1 3/1 3/1 3/1 

M e a n: 

Standard deviation: 

Number of observ. 

4061/M 4062/M 

4063/M 4064/M 

4/1 4/1 4/1 4/1 

564.5 523.3 540.7 541.2 523.2 549.9 509.9 505.6 

493.1 

537.6 528.9 

21.9 

(10) 

441.7 485.6 

507.2 

487.5 

522.2 509.9 

494.6 

557.9 549.5 

506.2 

35.2 

(9) 

501.2 530.0 

497.1 

530.2 517.9 

456.3 

628.4 

501.8 505.6 

475.9 

514.4 

46.0 

(10) 

505.3 

426.4 

542.9 504.2 

0.0676 0.0613 0.0755 0.0838 0.0739 0.0777 0.0702 0.0757 0.0647 

0.0745 0.0725 

0.0066 

(10) 

0.0786 0.0862 0. 0713 0.0751 0.0834 0.0774 0.0579 0.0672 0.0563 

0.0726 0.0105 

(9) 

0.0563 0.0646 0.0583 0.0738 0.0731 

0.0715 

0.0963 0.0712 

0.0541 

0.0610 

0.0680 

0.0123 

(10) 

0.0678 0.0697 0.0726 0.0832 

M a l e 

2.4063 2.1225 2.2540 

2.1570 

2.2516 2.2892 

2.2500 

2.1890 2.0485 2.1870 

2.2155 

0.0985 

(10) 

2.0432 2.1677 2.3236 2.0907 2.1000 

2.0610 

2.2039 2.0541 2.2852 

2.1477 

0.1038 

(9) 

2. 0871 2.2285 2.2277 2.1600 2.1983 

1.9884 

2.2298 2.1621 2.2104 2.1815 

2.1674 

0.0766 

(10) 

2.2471 2.0665 2.2383 

2.2660 

A n i m a l s 

1.8598 

1. 6593 

1.4214 

1. 6813 

1.3663 1.4992 1.5657 1.6763 1.3746 

1.6610 1.5765 

0.1600 

(10) 

1.3804 1.6101 

1. 7778 

1.3683 

2.0733 

1.6661 1.5475 1.4596 1.8291 

1.6347 

0.2308 

(9) 

1.5131 1.5347 

1. 7208 

1.5431 

1. 6042 

1.5880 1.4622 1.6400 1.4811 

0.2914 

1.4379 

0.4102 

(10) 

1.3042 

1. 5135 

1.6082 1.5339 

1.7694 

1. 8443 1. 6738 

1.9793 

1.7349 

1. 6046 

1.7320 

1. 5507 1. 6053 

1.9278 1.7422 

0.1418 

(10) 

1.5408 

1.6749 

1. 5448 1. 6006 1. 7223 

1.8562 

1.4287 1.7297 

1.8114 

1. 6566 

0.1391 

(9) 

1.8168 

1.8896 

1.5161 

1.6716 

1.5333 

1.5794 1.9941 

1.6123 

1.5273 

1.4732 

1. 6614 

0.1786 

(10) 

1.5751 1.3685 1.7095 

1. 5118 

Kidneys 

4.7984.9204.416

5.082

4.540

3.8303.896

3.928

3.673

4.3784.346

0.496

(10

3.589

4.1014.2774.3214.3934.463

3.948

4.2764.960

4.259

0.375

(9

4. 723 4.744

3.915

4.141

5.148

4.125

5.053

4.630

4.219

4.035

4.473

0. 441

(10

4.672

3.813

4.514

5.028
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal 

Summary statistics for absolute organ weights (g) 

Study number: 006130 

Brain Heart 

No/sex Subgroup Body wt. (g) Adrenal Glands Epididymis Kidneys 

-----------------------------------------------------------------------------------------------------------------

4065/M 4/1 497.7 0.0615 2.2338 1.5265 1. 4 763 4.3208 4066/M 4/1 526.6 0.0640 2.0945 1.6999 1.8448 4. 7114067/M 4/1 513.3 0.0712 2.2213 1.8551 1.6337 4.5666 4068/M 4/1 648.2 0.0826 2.1176 1. 8495 2.1954 5.2334069/M 4/1 458.3 0.0587 1.9940 1. 8152 1.3946 3.9382 4070/M 4/1 543.2 0.0701 2.1595 1. 5532 1. 7270 4.8858 

M e a n: 516.6 0.0701 2.1639 1. 6259 1.6437 4.5684 

Standard deviation: 58.7 0.0080 0.0921 0.1776 0.2459 0.4495 

Number of observ. : (10) (10) (10) (10) (10) (10
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal 

Summary statistics for absolute organ weights (g) 

Study number: 006130 

Prostate Spleen 

No/sex Subgroup Body wt. (g) Lungs Seminal vesicles 

1061/M 1/1 1062/M 1/1 1063/M 1/1 1064/M 1/1 1065/M 1/1 1066/M 1/1 1067/M 1/1 1068/M 1/1 1069/M 1/1 

1070/M 1/1 

M e a n: 

Standard deviation: 

Number of observ. 

2051/M 2052/M 

2053/M 2054/M 

2055/M 2056/M 2057/M 2058/M 2059/M 

2/1 2/1 2/1 2/1 2/1 2/1 2/1 

2/1 2/1 

M e a n: 

Standard deviation: 

Number of observ. 

3051/M 3052/M 3053/M 3054/M 3055/M 3056/M 3057/M 3058/M 3059/M 3060/M 

3/1 3/1 3/1 3/1 3/1 3/1 3/1 

3/1 

3/1 3/1 

M e a n: 

Standard deviation: 

Number of observ. 

4061/M 4062/M 4063/M 4064/M 

4/1 4/1 4/1 4/1 

564.5 523.3 540.7 541.2 523.2 549.9 509.9 505.6 

493.1 

537.6 

528.9 

21.9 

(10) 

441.7 485.6 

507.2 

487.5 

522.2 509.9 

494.6 

557.9 549.5 

506.2 

35.2 

(9) 

501.2 530.0 

497.1 

530.2 517.9 

456.3 

628.4 

501.8 505.6 

475.9 

514.4 

46.0 

(10) 

505.3 

426.4 

542.9 504.2 

2.8749 2.4519 2.1797 2.7940 2.3708 2.0346 2.0837 2.0613 2.1187 2.0381 

2.3008 

0.3150 

(10) 

2.0442 

1. 9518 

2.3282 2.2657 

1.9007 

2.2364 

1.9291 

1. 9442 

2.2458 

2.0940 

0.1722 

(9) 

1.8389 

2.4263 2.2576 2.1391 2.2686 2.0484 2.6291 

1.9559 1.9580 1.9780 

2.1500 

0.2456 

(10) 

2.0676 

1.6483 

2.0400 2.0895 

M a 1 e 

1.0387 

0. 9658 

1.3573 

1.2873 

1.7554 

0.7982 

1. 0436 

1.2206 1.0631 

0.8006 

1.1331 

0.2870 

(10) 

1.0224 

1. 2170 

1.0937 1.3740 1.1686 

0.8610 0.7979 0.9312 

1.2233 

1.0766 

0.1893 

(9) 

0.9867 

1.4637 1.5945 

0.8603 

1.2122 1.0245 1.1311 

1. 2989 

1.5916 

1.3287 

1. 2492 

0.2533 

(10) 

0.9868 

1.0217 1.2751 

0.9505 

A n i m a 1 s 

1.7028 

1. 8701 1. 2440 1. 5248 

1.9499 

2.2242 

1. 5742 

1.9077 

1. 4149 

1.8661 

1.7279 

0.2915 

(10) 

1.1152 

1. 5154 

1. 5052 

1.2733 

1. 5966 

1.1780 

1. 4852 

1.3045 1.8199 

1. 4215 

0.2231 

(9) 

2.2161 

1.1997 1.2646 

2.1342 2.5035 

1. 8020 1. 4989 

2.2541 

1.7969 

0.8360 

1.7506 

0.5404 

(10) 

1.4900 

1. 3550 

2.0265 2.2162 

0.7652 0.6641 

0.6345 0.8319 

0.8542 0.7046 0. 7195 0.7359 0.7758 

1.0586 

0.7744 0.1209 

(10) 

0.7329 0.9385 0.9693 

0.9129 

0.6463 

1.0285 

0.8044 0.8095 0.7348 

0.8419 0.1272 

(9) 

0.8195 

1.0280 

0.6120 0.9795 0.6773 0. 6964 0.7622 0.7652 0.7447 0.8663 

0.7951 0.1315 

(10) 

0.5725 0.5667 0.8970 0.6757 

Testes 

3.8803.5513.296

4.465

2.604

3.6983.350

3. 3 97

3.290

4.558

3.609

0.581

(10

3.1753.3743.8363.0553.5763.6003.8623.2863.089

3.428

0.305

(9

3.240

4.206

3.293

3.7203.8853.743

3.542

4.180

3.106

1.269

3.418

0.842

(10

3.589

4.069

3.754

3.583
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal 

Summary statistics for absolute organ weights (g) 

Study number: 006130 

Prostate Spleen 

No/sex Subgroup Body wt. (g) Lungs Seminal vesicles 

4065/M 4/1 497.7 1.7449 0.7834 1.8261 0.9435 4066/M 4/1 526.6 2.0652 1.5570 1.3858 0.8443 4067/M 4/1 513.3 2.1094 1.2787 1.1647 0.7463 4068/M 4/1 648.2 2.6291 1. 4548 1.8186 0.9193 4069/M 4/1 458.3 1.6757 1.1268 1.8678 0.6386 4070/M 4/1 543.2 1.9854 1.0819 2.2629 0.9476 

M e a n: 516.6 2.0055 1.1517 1. 7414 0.7752 

Standard deviation: 58.7 0.2828 0.2388 0.3770 0.1536 

Number of observ. : (10) (10) (10) (10) (10) 

Testes 

3.5833.8553.586

4.085

3.9683.2933.736

0.255

(10
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal 

Summary statistics for absolute organ weights (g) 

Study number: 006130 

Brain Kidneys 

No/sex Subgroup Body wt. (g) Adrenal Glands Heart 

1561/F 1/1 1562/F 1/1 

1563/F 1/1 

1564/F 1/1 

1565/F 1/1 1566/F 1/1 1567/F 1/1 1568/F 1/1 

1569/F 1/1 

1570/F 1/1 

M e a n: 

Standard deviation: 

Number of observ. 

2551/F 2552/F 

2553/F 2554/F 

2555/F 2556/F 2557/F 2558/F 2559/F 

2560/F 

2/1 2/1 

2/1 2/1 2/1 2/1 2/1 2/1 2/1 

2/1 

M e a n: 

Standard deviation: 

Number of observ. 

3551/F 3/1 3552/F 3/1 

3553/F 3/1 

3554/F 3/1 3555/F 3/1 3556/F 3/1 3557/F 3/1 3558/F 3/1 3559/F 3/1 

3560/F 3/1 

M e a n: 

Standard deviation: 

Number of observ. 

4561/F 4562/F 

4563/F 

4/1 4/1 

4/1 

290.5 291.0 292.3 

285.1 282.9 267.5 

297.3 

359.8 

282.6 

291.2 

294.0 

24.5 

(10) 

294.5 273.1 

281.8 287.0 287.5 

301.1 338.7 

249.0 262.0 

310.2 

288.5 

25.2 

(10) 

323.0 306;2 

265.7 

358.1 

288.4 

324.4 

267.1 

289.6 

352.2 

316.3 309.1 

32.0 

(10) 

302.8 

238.9 

265.1 

0.0567 0.0772 0.0762 

0.0552 

0.0708 0.0737 

0.0725 0.0636 0.0652 

0. 0718 0.0683 0.0078 

(10) 

0.0799 0.0978 

0.0888 0.0546 

0.0731 0.0773 0.0761 0.0666 0. 0716 

0.0819 0.0768 0.0118 

(10) 

0.0879 0.0953 

0.0550 0.0716 0.0569 0.0737 0.0641 0.0505 0.0521 

0.0675 

0.0675 0.0151 

(10) 

0.0738 0.0776 

0.0731 

F e m a 1 e 

1.9643 

1.9007 

2.0359 2.0639 2.0198 2.0881 2.0335 2.1674 

1.9637 

2.0447 

2.0282 

0.0739 

(10) 

2.0169 2.0800 

2.1760 2.0436 2.0994 2.0835 2.1288 

1.8170 

2 .1726 

2.0648 2.0683 

0.1021 

(10) 

2.1453 2.0739 2.2182 2.0652 

1.9817 

2.1663 2.0555 2.1400 2.1955 

1.9850 

2.1027 

0.0831 

(10) 

2.0233 2.0816 

2.0601 

A n i m a 1 s 

0.9904 0.9597 

1. 0142 

0.9561 

1.1183 

0.9976 0.9844 

1.2869 

1.0073 

1. 2504 

1.0565 

0.1207 

(10) 

1.1859 

1.0662 

1. 2248 

1.0525 

0.9184 

1.0531 

1.2013 

0.9334 

1.0031 

1.1833 1.0822 

0.1119 

(10) 

1.1221 

1.1910 

1.0215 

1. 3 049 

0.9747 

1.1055 

1.0800 

1. 0192 

1. 2093 

1.1544 

1.1183 

0.1006 

(10) 

1.0174 

0.9772 

0.9991 

2.3319 

1.9793 

2.2968 

2.2404 2.5332 2.5514 

2.2928 2.5304 2.3313 

2.5142 2.3602 

0.1791 

(10) 

2. 7772 2.7270 

2.5605 

2.0785 

2.2898 2.2906 2.9423 

1.8489 

2 .1180 

2.3242 

2.3957 

0.3473 

(10) 

3.2003 

2.9761 2.6512 2.7174 2.4327 2.6121 2.5210 2.3557 2.6128 

2.4639 

2.6543 

0.2585 

(10) 

2.4806 2.4411 

2.4708 

Liver 

7.8007.8417.444

6.869

8.846

9.045

8.218

9.936

7.707

9.417

8.312

0.964

(10

8.0478.304

8.9408.120

8.478

7.947

10.849

6.822

7.307

9.003

8.382

1.093

(10

9.7049.935

8.186

12.782

7.507

8.576

7.975

8.050

10.137

8.829

9.168

1.554

(10

8.859

8.269

9.047
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal 

Summary statistics for absolute organ weights (g) 

Study number: 006130 

Brain Kidneys 

No/sex Subgroup Body wt. (g) Adrenal Glands Heart 

4564/F 4/1 318.2 0.0780 2 .1092 1.1228 2.9107 4565/F 4/1 247.0 0.0549 1. 9549 0.8709 1.8795 4566/F 4/1 285.9 0.0625 2.1297 0.9804 2.4547 

4567/F 4/1 324.7 0.0846 2.1351 1 .1143 2.5823 

4568/F 4/1 280.1 0.0791 2.0966 0.8479 2.4035 

4569/F 4/1 299.5 0.0496 2.1919 1.0888 2.5291 4570/F 4/1 283.5 0.0623 2. 0119 1.0605 2.0879 

M e a n: 284.6 0.0696 2.0794 1.0079 2.4240 

Standard deviation: 28.3 0. 0115 0.0692 0.0943 0.2768 

Number of observ. : (10) (10) (10) (10) (10) 

Live

9.596

7.3967.913

11.366

8.567

9.057

8.178.825

1.096

(1
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal 

Summary statistics for absolute organ weights (g) 

Study number: 006130 

Spleen Uterus 

No/sex Subgroup Body wt. (g) Ovaries Thymus 

F e m a 1 e A n i m a 1 s 

1561/F 1/1 290.5 0.1175 0. 6116 0.4340 0.7637 1562/F 1/1 291.0 0.1075 0.5311 0.3376 1.2560 1563/F 1/1 292.3 0.0982 0.5895 0.2959 0.8180 1564/F 1/1 285.1 0.0826 0.5224 0.2562 1.1473 1565/F 1/1 282.9 0.0988 0.5805 0.2948 0.9121 

1566/F 1/1 267.5 0.0987 0.5588 0.2922 0.7364 1567/F 1/1 297.3 0.0950 0.6376 0.3105 0.9374 1568/F 1/1 359.8 0.0858 0.5481 0.3124 0.8851 1569/F 1/1 282.6 0.0806 0.6837 0.3541 0.6695 1570/F 1/1 291.2 0.0639 0.5043 0.3019 0.8581 

M e a n: 294.0 0.0929 0.5768 0.3190 0.8984 

Standard deviation: 24.5 0.0152 0.0557 0.0483 0.1815 

Number of observ. (10) (10) (10) (10) (10) 

2551/F 2/1 294.5 0.1159 0.6205 0.3603 0.7080 

2552/F 2/1 273.1 0.0830 0.5184 0.2951 0.6536 2553/F 2/1 281.8 0.0877 0.4849 0.3175 0.6131 2554/F 2/1 287.0 0.1133 0.5825 0.3433 0.7749 2555/F 2/1 287.5 0.0924 0.5644 0.4026 0.6564 2556/F 2/1 301.1 0.0828 0.5058 0.3215 0.7290 2557/F 2/1 338.7 0.0696 0.6234 0.2641 0.7378 2558/F 2/1 249.0 0.0947 0.4954 0.2642 0.5761 2559/F 2/1 262.0 0.0863 0.3964 0.4146 0.6915 

2560/F 2/1 310.2 0.0952 0.7102 0.3538 0. 7191 

M e a n: 288.5 0.0921 0.5502 0.3337 0.6860 

Standard deviation: 25.2 0.0140 0.0889 0.0517 0.0609 

Number of observ. : (10) (10) (10) (10) (10) 

3551/F 3/1 323.0 0.1061 0.6653 0.3457 0.6784 3552/F 3/1 306.2 0 .1166 0. 5966 0.3832 0.6139 3553/F 3/1 265.7 0.0942 0.5239 0.2295 0.8359 3554/F 3/1 358.1 0.0652 0. 7174 0.3376 0.8513 3555/F 3/1 288.4 0.0955 0.6891 0.3376 0.8370 3556/F 3/1 324.4 0.0928 0.5748 0.4736 1.5785 3557/F 3/1 267.1 0.0692 0.4306 0.3119 0.8597 3558/F 3/1 289.6 0.0830 0.5067 0.3132 1.7885 3559/F 3/1 352.2 0.1007 0.5914 0.3320 0.8650 

3560/F 3/1 316.3 0. 0913 0.5855 0.3281 1.1408 

M e a n: 309.1 0.0915 0.5881 0.3392 1.0049 

Standard deviation: 32.0 0.0157 0.0874 0.0612 0.3861 

Number of observ. : (10) (10) (10) (10) (10) 

4561/F 4/1 302.8 0.0989 0.7258 0.3519 0.6059 4562/F 4/1 238.9 0.1062 0.4973 0.3957 0.8783 4563/F 4/1 265.1 0.0887 0.5980 0.2658 0.6781 
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal 

Summary statistics for absolute organ weights (g) 

Study number: 006130 

Spleen Uterus 

No/sex Subgroup Body wt. (g) Ovaries Thymus 

4564/F 4/1 318.2 0 .1101 0.5714 0.2940 0.5814 

4565/F 4/1 247.0 0.0678 0.5418 0.1421 0. 7718 4566/F 4/1 285.9 0.0859 0.4713 0.2804 0.6585 4567/F 4/1 324.7 0.0790 0.5985 0.3560 0.9037 4568/F 4/1 280.1 0. 0771 0.6547 0.3270 0.8758 4569/F 4/1 299.5 0.0920 0.5199 0.2821 0.6079 4570/F 4/1 283.5 0.0842 0.5920 0.2955 1.4417 

M e a n: 284.6 0.0890 0.5771 0.2991 0.8003 

Standard deviation: 28.3 0.0132 0.0755 0.0688 0.2566 

Number of observ. : (10) (10) (10) (10) (10) 
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal 

Summary Statistics for % Organ to Body Weight 

Study number: 006130 

Brain Heart 

No/sex Subgroup Body wt. (g) Adrenal Glands Epididymis 

1061/M 1/1 1062/M 1/1 

1063/M 1/1 

1064/M 1/1 

1065/M 1/1 1066/M 1/1 1067/M 1/1 1068/M 1/1 1069/M 1/1 1070/M 1/1 

M e a n: 

Standard deviation: 

Number of observ. 

2051/M 2052/M 2053/M 2054/M 2055/M 2056/M 2057/M 2058/M 2059/M 

2/1 2/1 2/1 2/1 2/1 2/1 2/1 2/1 2/1 

M e a n: 

Standard deviation: 

Number of observ. 

3051/M 3052/M 3053/M 3054/M 3055/M 3056/M 3057/M 

3058/M 3059/M 3060/M 

3/1 3/1 3/1 3/1 3/1 3/1 

3/1 3/1 3/1 3/1 

M e a n: 

Standard deviation: 

Number of observ. 

4061/M 4062/M 

4063/M 4064/M 

4/1 4/1 4/1 4/1 

564.5 523.3 540.7 541.2 523.2 549.9 509.9 505.6 

493.1 

537.6 528.9 

21.9 

(10) 

441.7 485.6 

507.2 

487.5 

522.2 509.9 

494.6 

557.9 549.5 

506.2 

35.2 

(9) 

501.2 

530.0 

497.1 

530.2 517.9 

456.3 

628.4 

501.8 505.6 

475.9 

514.4 

46.0 

(10) 

505.3 

426.4 

542.9 504.2 

0.0120 0. 0117 

0.0140 0.0155 0.0141 0.0141 0.0138 0.0150 0.0131 0.0139 0.0137 0.0012 

(10) 

0.0178 0.0178 0.0141 0.0154 0.0160 

0.0152 

0. 0117 0.0120 

0.0102 

0.0145 0.0027 

(9) 

0. 0112 0.0122 0. 0117 0.0139 0.0141 0.0157 0.0153 0.0142 0.0107 0.0128 

0. 0132 

0.0017 

(10) 

0.0134 0.0163 0.0134 0.0165 

M a l e 

0.4263 0.4056 0.4169 0.3986 0.4304 0.4163 0.4413 0.4330 0.4154 0.4068 0.4190 0. 0135 

(10) 

0.4626 0.4464 0. 4581 0.4289 0. 4021 0. 4042 0.4456 0.3682 0.4159 0.4258 0.0310 

(9) 

0.4164 0.4205 0.4481 0.4074 0.4245 0.4358 0.3548 

·o .4309 

0.4372 0.4584 0.4234 0.0284 

(10) 

0.4447 0.4846 0.4123 0.4494 

A n i m a l s 

0.3295 0.3171 0.2629 0.3107 0.2611 

0. 2726 0.3071 0.3315 0.2788 

0.3090 

0.2980 0.0268 

(10) 

0.3125 0.3316 

0.3505 0.2807 0.3970 0.3268 0.3129 0.2616 0.3329 0.3229 0.0390 

(9) 

0.3019 0.2896 0.3462 

0.2910 

0.3098 

0.3480 0.2327 0.3268 0.2929 0.0612 0.2800 0.0837 

(10) 

0.2581 0.3549 0.2962 0.3042 

0.3134 0.3524 0.3096 

0.3657 0.3316 0.2918 0.3397 0.3067 0.3256 0.3586 0.3295 0.0245 

(10) 

0.3488 0.3449 0.3046 0.3283 0.3298 0.3640 0.2889 0.3100 0.3296 0.3277 0.0235 

(9) 

0.3625 0.3565 0.3050 0.3153 0.2961 0.3461 0.3173 0.3213 

0.3021 0.3096 0.3232 0.0235 

(10) 

0.3117 0.3209 0.3149 0.2998 

Kidney

0.850.940.810.930.860.69

0.760.77

0.74

0.810.820.08

(1

0.810.840.840.880.840.870.790.760.900.840.040.94

0.89

0.780. 780.99

0.900.800.920.83

0.840. 870. 07

(1

0.920.890.830.99
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal 

Summary Statistics for % Organ to Body Weight 

Study number: 006130 

Brain Heart 

No/sex Subgroup Body wt. (g) Adrenal Glands Epididymis 

4065/M 4/1 497.7 0.0124 0.4488 0.3067 0.2966 4066/M 4/1 526.6 0.0122 0.3977 0.3228 0.3503 

4067/M 4/1 513.3 0.0139 0.4327 0.3614 0.3183 4068/M 4/1 648.2 0.0127 0.3267 0.2853 0.3387 4069/M 4/1 458.3 0.0128 0.4351 0.3961 0.3043 4070/M 4/1 543.2 0.0129 0.3976 0.2859 0.3179 

M e a n: 516.6 0.0136 0.4230 0.3172 0.3174 

Standard deviation: 58.7 0.0015 0.0429 0.0419 0.0167 

Number of observ. : (10) (10) (10) (10) (10) 

Kidneys 

0.8680.8940.8890.8070.8590.8990.8860.052

(10
Huntingdon Life Sciences Summary Statistics for % Organ to Body Weight 

Princeton Research Center Study number: 006130 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal Prostate Spleen 

No/sex Subgroup Body wt. (g) Lungs Seminal vesicles 

1061/M 1062/M 1063/M 1064/M 1065/M 

1066/M 1067/M 1068/M 1069/M 1070/M 

1/1 1/1 1/1 1/1 1/1 1/1 

1/1 

1/1 1/1 1/1 

M e a n: 

Standard deviation: 

Number of observ. 

2051/M 2052/M 2053/M 2054/M 2055/M 2056/M 2057/M 2058/M 2059/M 

2/1 2/1 2/1 2/1 2/1 2/1 2/1 2/1 2/1 

M e a n: 

Standard deviation: 

Number of observ. 

3051/M 3052/M 3053/M 3054/M 3055/M 3056/M 3057/M 3058/M 3059/M 3060/M 

3/1 3/1 3/1 3/1 3/1 3/1 3/1 3/1 3/1 3/1 

M e a n: 

Standard deviation: 

Number of observ. 

4061/M 4062/M 4063/M 

4064/M 

4/1 4/1 4/1 4/1 

564.5 

523.3 

540.7 541.2 523.2 549.9 509.9 505.6 

493.1 

537.6 528.9 

21.9 

(10) 

441.7 

485.6 

507.2 

487.5 

522.2 

509.9 

494.6 

557.9 

549.5 506.2 

35.2 

(9) 

501.2 530.0 

497.1 

530.2 517.9 

456.3 

628.4 

501.8 

505.6 

475.9 

514.4 

46.0 

(10) 

505.3 

426.4 

542.9 

504.2 

0.5093 0.4685 

0.4031 0.5163 0.4531 0.3700 0.4086 0.4077 0.4297 0.3791 0.4345 0.0510 

(10) 

0.4628 0.4019 

0.4590 

0.4648 

0.3640 0.4386 0.3900 0.3485 0.4087 0.4154 0.0435 

(9) 

0.3669 0.4578 0.4542 0.4035 0.4380 0.4489 0.4184 0.3898 

0.3873 0.4156 0.4180 0.0313 

(10) 

0.4092 0.3866 0.3758 

0.4144 

M a 1 e 

0.1840 0.1846 0.2510 0.2379 0.3355 0.1452 0.2047 0.2414 0.2156 0.1489 

0.2149 0.0561 

(10) 

0.2315 0.2506 0.2156 0.2818 0.2238 0.1689 

0.1613 

0.1669 0.2226 0.2137 0.0410 

(9) 

0.1969 0.2762 0.3208 0.1623 

0.2341 

0.2245 0.1800 0.2588 0.3148 0.2792 0.2447 0.0546 

(10) 

0.1953 

0.2396 0.2349 

0.1885 

A n i m a 1 s 

0.3016 0.3574 0.2301 0.2817 0.3727 0.4045 0.3087 0.3773 0.2869 0.3471 0.3268 0.0537 

(10) 

0.2525 0.3121 0.2968 0.2612 

0.3057 0.2310 

0.3003 

0.2338 0.3312 0.2805 0.0365 

(9) 

0.4422 0.2264 0.2544 0.4025 0.4834 0. 3 949 0.2385 0.4492 

0.3554 0.1757 

0.3423 

0.1093 

(10) 

0.2949 0.3178 0.3733 

0.4395 

0.1356 0.1269 

0.1173 0.1537 0.1633 0.1281 0.1411 0.1455 0.1573 

0.1969 0.1466 0.0229 

(10) 

0.1659 0.1933 0.1911 0.1873 0.1238 0.2017 0.1626 0.1451 0.1337 0.1672 0.0282 

(9) 

0.1635 0.1940 0.1231 0.1847 0.1308 0.1526 0.1213 0.1525 0.1473 0.1820 0.1552 0.0259 

(10) 

0.1133 0.1329 0.1652 0.1340 

Testes 

0.6870.6780.6090.8250.4970. 672

0.6570.6710.6670.8470.6810.099

(10

0.7190.6940.7560.6260.6840.7060.7800.5890.5620.6800.073

(9

0.6460.7930.6620.7010.7500.8200.5630.8330.6140.266

0.6650.166

(10

0.7100.9540.6910. 710
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal 

Animal Group/ Terminal 

No/sex Subgroup Body wt. (g) 

4065/M 4/1 497.7 4066/M 4/1 526.6 4067/M 4/1 513.3 4068/M 4/1 648.2 4069/M 4/1 458.3 4070/M 4/1 543.2 

M e a n: 516.6 

Standard deviation: 58.7 

Number of observ. : (10) 

Sacrifice 

Summary Statistics for % Organ to Body Weight 

Study number: 006130 

Prostate Spleen 

Lungs Seminal vesicles 

0.3506 0.1574 0.3669 0.1896 

0.3922 0.2957 0.2632 0.1603 

0.4109 0.2491 0.2269 0.1454 

0.4056 0.2244 0.2806 0.1418 

0.3656 0.2459 0.4075 0.1393 

0.3655 0.1992 0.4166 0.1744 

0.3876 0.2230 0.3387 0.1496 

0.0225 0.0391 0.0722 0.0226 

(10) (10) (10) (10) 

Teste

0.7190.73

0.69

0.630

0.865

0.600.7320.104

(1
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal 

Summary Statistics for % Organ to Body Weight 

Study number: 006130 

Brain Kidneys 

No/sex Subgroup Body wt. (g) Adrenal Glands Heart 

1561/F 1562/F 1563/F 1564/F 

1565/F 1566/F 1567/F 1568/F 1569/F 

1570/F 

1/1 1/1 1/1 1/1 1/1 

1/1 

1/1 

1/1 1/1 

1/1 

M e a n: 

Standard deviation: 

Number of observ. 

2551/F 2552/F 2553/F 2554/F 2555/F 2556/F 2557/F 2558/F 2559/F 2560/F 

2/1 

2/1 

2/1 2/1 2/1 2/1 2/1 2/1 2/1 

2/1 

M e a n: 

Standard deviation: 

Number of observ. 

3551/F 3/1 

3552/F 3/1 3553/F 3/1 3554/F 3/1 3555/F 3/1 3556/F 3/1 3557/F 3/1 3558/F 3/1 3559/F 3/1 

3560/F 3/1 

M e a n: 

Standard deviation: 

Number of observ. 

4561/F 4562/F 

4563/F 

4/1 4/1 4/1 

290.5 

291.0 292.3 285.1 282.9 267.5 297.3 

359.8 

282.6 291.2 

294.0 

24.5 

(10) 

294.5 273.1 

281.8 

287.0 287.5 

301.1 

338.7 

249.0 262.0 

310.2 

288.5 

25.2 

(10) 

323.0 306.2 

265.7 

358.1 

288.4 

324.4 

267.1 289.6 

352.2 316.3 

309.1 

32.0 

(10) 

302.8 

238.9 265.1 

0.0195 

0.0265 0.0261 0.0194 0.0250 0.0276 0.0244 0.0177 0.0231 0.0247 

0.0234 0.0034 

(10) 

0. 0271 0.0358 0.0315 0.0190 0.0254 0.0257 0.0225 0.0267 0.0273 

0.0264 0.0268 0.0045 

(10) 

0. 0272 

0. 0311 

0.0207 

0.0200 

0.0197 

0.0227 0.0240 0.0174 0.0148 

0.0213 0.0219 0.0047 

(10) 

0.0244 0.0325 0.0276 

F e m a 1 e 

0.6762 0.6532 

0.6965 

0. 7239 

0.7140 0.7806 0.6840 0.6024 0.6949 0.7022 

0.6928 0.0463 

(10) 

0.6849 0.7616 0.7722 0.7121 0.7302 0.6920 

0.6285 

0.7297 

0.8292 

0.6656 0. 7206 0.0577 

(10) 

0.6642 0.6773 0.8349 0.5767 0.6871 0.6678 0. 7696 0.7390 0.6234 

0.6276 

0.6867 

0.0761 

(10) 

0.6682 0.8713 0.7771 

A n i m a 1 s 

0.3409 0.3298 0.3470 0.3354 0.3953 0.3729 0.3311 0.3577 0.3564 0.4294 

0.3596 

0.0319 

(10) 

0.4027 0.3904 0.4346 0.3667 0.3194 0.3498 0.3547 0.3749 0.3829 

0.3815 

0.3758 0.0314 

(10) 

0.3474 0.3890 0.3845 0.3644 

0.3380 

0.3408 0.4043 0.3519 0.3434 

0.3650 0.3629 0.0229 

(10) 

0.3360 0.4090 0.3769 

0.8027 0.6802 0.7858 0.7858 0.8954 0.9538 0. 7712 0.7033 0.8249 0.8634 

0.8067 0.0829 

(10) 

0.9430 0.9985 

0.9086 

0. 7242 

0.7965 

0.7607 0.8687 0.7425 0.8084 

0.7493 0.8300 0.0946 

(10) 

0.9908 0. 9719 0.9978 0.7588 0.8435 0.8052 0.9438 0. 8134 

0.7419 

0.7790 0.8646 0.1009 

(10) 

0.8192 

1.0218 

0.9320 

Live

2.6852.6942.5462.409

3.126

3.381

2.764

2.761

2.727

3.234

2.833

0.311

(10

2. 732

3.040

3 .172

2.8292.9482.639

3.203

2.7392.789

2.9022.899

0.190

(10

3.0043.244

3.0813.569

2.6032.6432.9852.7802.878

2.7912.958

0.291

(1

2.925

3.4613.412
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal 

Animal Group/ Terminal 

No/sex Subgroup Body wt. (g) 

4564/F 4/1 318.2 4565/F 4/1 247.0 4566/F 4/1 285.9 4567/F 4/1 324.7 4568/F 4/1 280.1 4569/F 4/1 299.5 4570/F 4/1 283.5 

M e a n: 284.6 

Standard deviation: 28.3 

Number of observ. : (10) 

Sacrifice 

Summary Statistics for % Organ to Body Weight 

Study number: 006130 

Brain Kidneys 

Adrenal Glands Heart 

0.0245 0.6629 0.3529 0.9147 0.0222 0.7915 0.3526 0.7609 

0.0219 0.7449 0.3429 0.8586 0.0261 0.6576 0.3432 0.7953 

0.0282 0.7485 0.3027 0.8581 

0.0166 0.7319 0.3635 0.8444 

0.0220 0.7097 0.3741 0.7365 

0.0246 0.7363 0.3554 0.8542 

0.0044 0.0668 0.0283 0.0851 

(10) (10) (10) (10) 

Liver 

3.015

2.994

2.767

3.5003.0583.024

2.884

3.104

0.258

(10
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal 

Animal Group/ Terminal 

No/sex Subgroup Body wt. (g) 

1561/F 1/1 290.5 

1562/F 1/1 291.0 1563/F 1/1 292.3 1564/F 1/1 285.1 

1565/F 1/1 282.9 

1566/F 1/1 267.5 1567/F 1/1 297.3 1568/F 1/1 359.8 

1569/F 1/1 282.6 1570/F 1/1 291.2 

M e a n: 294.0 

Standard deviation: 24.5 

Number of observ. : (10) 

2551/F 2/1 294.5 2552/F 2/1 273.1 2553/F 2/1 281.8 2554/F 2/1 287.0 2555/F 2/1 287.5 

2556/F 2/1 301.1 2557/F 2/1 338.7 2558/F 2/1 249.0 2559/F 2/1 262.0 2560/F 2/1 310.2 

M e a n: 288.5 

Standard deviation: 25.2 

Number of observ. : (10) 

3551/F 3/1 323.0 3552/F 3/1 306.2 3553/F 3/1 265.7 3554/F 3/1 358.1 3555/F 3/1 288.4 3556/F 3/1 324.4 3557/F 3/1 267.1 3558/F 3/1 289.6 3559/F 3/1 352.2 3560/F 3/1 316.3 

M e a n: 309.1 

Standard deviation: 32.0 

Number of observ. : (10) 

4561/F 4/1 302.8 4562/F 4/1 238.9 4563/F 4/1 265.1 

Sacrifice 

Summary Statistics for % Organ to Body Weight 

Study number: 006130 

Spleen Uterus 

Ovaries Thymus 

F e m a l e A n i m a l s 

0.0404 0.2105 0.1494 0.2629 0.0369 0.1825 0.1160 0.4316 

0.0336 0.2017 0.1012 0.2798 

0.0290 0.1832 0.0899 0.4024 0.0349 0.2052 0.1042 0.3224 0.0369 0.2089 0.1092 0.2753 0.0320 0.2145 0.1044 0.3153 

0.0238 0.1523 0.0868 0.2460 

0.0285 0.2419 0.1253 0.2369 

0.0219 0.1732 0.1037 0.2947 

0.0318 0.1974 0.1090 0.3067 0.0060 0.0252 0.0181 0.0645 

(10) (10) (10) (10) 

0.0394 0.2107 0.1223 0.2404 

0.0304 0.1898 0.1081 0.2393 0. 0311 0.1721 0.1127 0.2176 0.0395 0.2030 0.1196 0.2700 0.0321 0.1963 0.1400 0.2283 0.0275 0.1680 0.1068 0.2421 0.0205 0.1841 0.0780 0.2178 

0.0380 0.1990 0.1061 0.2314 

0.0329 0.1513 0.1582 0.2639 

0.0307 0.2289 0.1141 0.2318 

0.0322 0.1903 0.1166 0.2383 

0.0058 0.0225 0.0214 0.0174 

(10) (10) (10) (10) 

0.0328 0.2060 0.1070 0.2100 

0.0381 0.1948 0.1251 0.2005 

0.0355 0.1972 0.0864 0.3146 

0.0182 0.2003 0.0943 0.2377 

0.0331 0.2389 0.1171 0.2902 

0.0286 0.1772 0.1460 0.4866 

0.0259 0.1612 0.1168 0.3219 

0.0287 0.1750 0.1081 0.6176 

0.0286 0.1679 0.0943 0.2456 

0.0289 0.1851 0.1037 0.3607 

0.0298 0.1904 0.1099 0.3285 0.0055 0.0225 0.0174 0.1320 

(10) (10) (10) (10) 

0.0327 0.2397 0.1162 0.2001 0.0445 0.2082 0.1656 0.3676 

0.0335 0.2256 0.1003 0.2558 
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal 

Summary Statistics for % Organ to Body Weight 

Study number: 006130 

Spleen Uterus 

No/sex Subgroup Body wt. (g) Ovaries Thymus 

4564/F 4/1 318.2 0.0346 0.1796 0.0924 0.1827 

4565/F 4/1 247.0 0.0274 0.2194 0.0575 0.3125 4566/F 4/1 285.9 0.0300 0.1648 0.0981 0.2303 4567/F 4/1 324.7 0.0243 0.1843 0.1096 0.2783 4568/F 4/1 280.1 0.0275 0.2337 0.1167 0.3127 4569/F 4/1 299.5 0.0307 0.1736 0.0942 0.2030 4570/F 4/1 283.5 0.0297 0.2088 0.1042 0.5085 

M e a n: 284.6 0.0315 0.2038 0.1055 0.2852 

Standard deviation: 28.3 0.0055 0.0265 0.0270 0.0981 

Number of observ. : (10) (10) (10) (10) (10) 
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal 

Summary Statistics for % Organ to Brain Weight 

Study number: 006130 

Brain Heart 

No/sex Subgroup Body wt. (g) Adrenal Glands Epididymis 

1061/M 1062/M 1063/M 1064/M 1065/M 1066/M 1067/M 1068/M 1069/M 

1070/M 

1/1 1/1 

1/1 

1/1 1/1 1/1 1/1 1/1 1/1 1/1 

M e a n: 

Standard deviation: 

Number of observ. 

2051/M 2052/M 2053/M 2054/M 2055/M 2056/M 2057/M 2058/M 2059/M 

2/1 2/1 2/1 2/1 2/1 2/1 

2/1 

2/1 2/1 

M e a n: 

Standard deviation: 

Number of observ. 

3051/M 3/1 3052/M 3/1 3053/M 3/1 3054/M 3/1 3055/M 3/1 3056/M 3/1 3057/M 3/1 3058/M 3/1 3059/M 3/1 3060/M 3/1 

M e a n: 

Standard deviation: 

Number of observ. 

4061/M 4062/M 4063/M 4064/M 

4/1 4/1 4/1 4/1 

564.5 523.3 540.7 541.2 

523.2 549.9 509.9 505.6 

493.1 

537.6 528.9 

21.9 

(10) 

441.7 485.6 

507.2 

487.5 

522.2 

509.9 

494.6 

557.9 549.5 506.2 

35.2 

(9) 

501.2 530.0 

497.1 

530.2 517.9 

456.3 

628.4 

501.8 505.6 

475.9 

514.4 

46.0 

(10) 

505.3 

426.4 

542.9 504.2 

2.8093 2.8881 

3.3496 3.8850 3.2821 

3. 3 942 

3.1200 3.4582 3.1584 3.4065 3.2751 

0.3068 

(10) 

3.8469 3.9766 3.0685 3.5921 3.9714 3.7555 

2. 6272 

3.2715 

2.4637 

3.3970 

0. 5725 

(9) 

2.6975 2.8988 2.6170 

3.4167 3.3253 3.5959 

4.3188 

3.2931 

2.4475 2.7962 

3.1407 

0.5634 

(10) 

3.0172 3.3729 3.2435 3.6717 

M a 1 e 

100.0000 100.0000 100.0000 100.0000 100.0000 

100.0000 

100.0000 

100.0000 100.0000 100.0000 100.0000 

0.0000 

(10) 

100.0000 100.0000 100.0000 100.0000 

100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 

0.0000 

(9) 

100.0000 100.0000 

100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 

0.0000 

(10) 

100.0000 100.0000 100.0000 100.0000 

A n i m a 1 s 

77.2888 78.1767 

63.0612 

77.9462 

60.6813 65.4901 69.5867 

76.5784 

67.1028 

75.9488 71.1861 

6.7627 

(10) 

67.5607 

74.2769 76.5106 

65.4470 

98.7286 

80.8394 

70.2164 71.0579 

80.0411 

76.0754 

9.9999 

(9) 

72.4977 

68.8670 

77.2456 71.4398 72.9746 79.8632 

65.5754 

75.8522 

67.0060 

13.3578 

66.4679 

19.1909 

(10) 

58.0393 

73.2398 71.8492 

67.6920 

73.5320 

86.8928 

74.2591 

91.7617 

77.0519 70.0944 76.9778 70.8406 78.3647 

88.1482 

78.7923 

7.5643 

(10) 

75.4111 77.2662 

66.4830 

76.5581 

82.0143 

90.0631 

64.8260 

84.2072 

79.2666 

77.3440 

8. 0110 

(9) 

87.0490 84.7925 

68.0567 

77.3889 

69.7494 

79.4307 

89.4296 

74.5710 

69.0961 

67.5315 

76.7095 

8.2462 

(10) 

70.0948 

66.2231 

76.3749 

66.7167 

Kidneys 

199.401

231.830

195.958

235.605

201.674

167.316173.155179.442179.306

200.182

196.387

23.117

(10

175.695189.214184.085

206.720

209.214216.579

179.150

208.173217.066

198.433

16.340

(9

226.328212.901

175.750191.726

234.221

207.463

226.643214.157

190.897185.005

206.509

19.824

(10

207.921

184.514

201.679221.915
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal 

Summary Statistics for % Organ to Brain Weight 

Study number: 006130 

Brain Heart 

No/sex Subgroup Body wt. (g) Adrenal Glands Epididymis 

4065/M 4/1 497.7 2.7532 100.0000 68.3365 66.0892 4066/M 4/1 526.6 3.0556 100.0000' 81.1602 88.0783 4067/M 4/1 513.3 3.2053 100.0000 83.5142 73.5470 4068/M 4/1 648.2 3.9006 100.0000 87.3394 103.6740 4069/M 4/1 458.3 2.9438 100.0000 91.0331 69.9398 4070/M 4/1 543.2 3.2461 100.0000 71.9241 79.9722 

M e a n: 516.6 3.2410 100.0000 75.4128 76.0710 

Standard deviation: 58.7 0.3416 0.0000 10.1435 11.9499 

Number of observ. : (10) (10) (10) (10) (10) 

Kidneys 

193.428

224.927205.582247.143

197.502

226.246211.086

18.767

(10
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Summary Statistics for % Organ to Brain Weight 

Study number: 006130 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal 

No/sex Subgroup Body wt. (g) Lungs 

1061/M 1/1 1062/M 1/1 1063/M 1/1 1064/M 1/1 

1065/M 1/1 

1066/M 1/1 

1067/M 1/1 

1068/M 1/1 1069/M 1/1 

1070/M 1/1 

M e a n: 

Standard deviation: 

Number of observ. 

2051/M 2/1 2052/M 2/1 2053/M 2/1 2054/M 2/1 2055/M 2/1 2056/M 2/1 

2057/M 2/1 2058/M 2/1 

2059/M 2/1 

M e a n: 

Standard deviation: 

Number of observ. 

3051/M 3/1 3052/M 3/1 

3053/M 3/1 3054/M 3/1 3055/M 3/1 3056/M 3/1 3057/M 3/1 3058/M 3/1 3059/M 3/1 3060/M 3/1 

M e a n: 

Standard deviation: 

Number of observ. 

4061/M 4062/M 4063/M 4064/M 

4/1 4/1 4/1 4/1 

564.5 523.3 

540.7 

541.2 523.2 549.9 509.9 505.6 

493.1 

537.6 528.9 

21.9 

(10) 

441.7 485.6 

507.2 

487.5 

522.2 

509.9 

494.6 

557.9 549.5 506.2 

35.2 

(9) 

501.2 530.0 

497.1 

530.2 517.9 

456.3 

628.4 

501.8 505.6 

475.9 

514.4 

46.0 

(10) 

505.3 

426.4 

542.9 504.2 

119.4739 115.5194 

96.7036 

129.5318 105.2940 

88.8782 

92.6089 94.1663 

103.4269 

93.1916 

103.8795 

13.5444 

(10) 

100.0490 

90.0401 

100.1980 108.3704 

90.5095 

108.5104 

87.5312 

94.6497 98.2759 97.5705 

7.6301 

(9) 

88.1079 

108.8759 

101.3422 

99.0324 

103.1979 103.0175 117.9074 

90.4630 

88.5812 

90.6716 99.1197 

9.7955 

(10) 

92.0119 

79.7629 

91.1406 92.2110 

Prostate 

Seminal vesicles 

M a 1 e 

43.1659 45.5029 

60.2174 

59.6801 

77.9623 

34.8681 

46.3822 

55.7606 51.8965 

36.6072 

51.2043 

12.8696 

(10) 

50.0392 56.1425 

47.0692 

65.7196 

55.6476 

41.7758 

36.2040 

45.3337 

53.5314 50.1626 

8.7842 

(9) 

47.2761 

65.6810 

71.5761 

39.8287 

55.1426 

51.5238 50.7265 

60.0759 

72.0051 

60.9076 

57.4743 

10.5286 

(10) 

43.9144 49.4411 

56.9673 

41.9462 

A n i m a 1 s 

70.7643 

88.1084 

55.1908 

70.6908 

86.6006 

97.1606 

69.9645 

87.1494 

69.0701 

85.3269 

78.0026 

12.6975 

(10) 

54.5811 

69.9082 64.7788 60.9031 

76.0286 

57.1567 

67.3896 63.5071 

79.6385 

65.9880 

8.2619 

(9) 

106.1808 

53.8344 56.7671 

98.8056 

113.8835 

90.6256 

67.2213 

104.2551 

81.2930 

38.3223 

81.1189 

25.8248 

(10) 

66.3077 65.5698 

90.5375 97.8023 

Spleen 

31.7999 31.2886 

28.1500 

38.5675 

37.9375 30.7793 31.9778 33.6181 

37.8716 

48.4042 

35.0394 

5.8600 

(10) 

35.8702 

43.2947 41.7154 43.6648 

30.7762 

49.9030 

36.4989 39.4090 32.1547 39.2541 

6.0887 

(9) 

39.2650 

46.1297 

27.4723 

45.3472 

30.8102 35.0231 34.1824 35.3915 33.6907 39.7112 36.7023 

5.9544 

(10) 

25.4773 27.4232 

40.0751 

29.8191 

Testes 

161.264167.335146.255

207.000

115.664161.554

148.928

155.189160.649

208.413

163.225

27.502

(10

155.437155.662165.127

146.132

170.323174.677

175.257159.972135.182

159.752

13.362

(9

155.248188.777147.856172.236

176.750188.282158.857193.344140.553

58.198

158.010

39.501

(10

159.730196.946167.721158.155
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal 

Animal Group/ Terminal 

No/sex Subgroup Body wt. (g) 

4065/M 4/1 497.7 

4066/M 4/1 526.6 

4067/M 4/1 513.3 4068/M 4/1 648.2 4069/M 4/1 458.3 4070/M 4/1 543.2 

M e a n: 516.6 

Standard deviation: 58.7 

Number of observ. : (10) 

Summary Statistics for % Organ to Brain Weight 

Study number: 006130 

Sacrifice 

Prostate Spleen 

Lungs Seminal vesicles 

78.1135 35.0703 81.7486 42.2374 

98.6011 74.3376 66.1638 40.3103 94.9624 57.5654 52.4333 33.5974 

124.1547 68.7004 85.8802 43.4124 

84.0371 56.5095 93.6710 32.0261 

91.9380 50.0996 104.7882 43.8805 92.6933 53.4552 80.4902 35.8259 

12.8593 11.9996 17.0347 6.9513 

(10) (10) (10) (10) 

Teste

160.40184.05161.45192.92199.02152.48173.29

17.96

(1
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal 

Animal Group/ Terminal 

No/sex Subgroup Body wt. (g) 

1561/F 1/1 1562/F 1/1 1563/F 1/1 

1564/F 1/1 1565/F 1/1 1566/F 1/1 1567/F 1/1 1568/F 1/1 1569/F 1/1 1570/F 1/1 

M e a n: 

Standard deviation: 

Number of observ. 

2551/F 2552/F 2553/F 2554/F 2555/F 2556/F 2557/F 2558/F 2559/F 2560/F 

2/1 2/1 2/1 2/1 2/1 2/1 

2/1 

2/1 2/1 

2/1 

M e a n: 

Standard deviation: 

Number of observ. 

3551/F 3/1 3552/F 3/1 3553/F 3/1 3554/F 3/1 

3555/F 3/1 3556/F 3/1 3557/F 3/1 3558/F 3/1 3559/F 3/1 3560/F 3/1 

M e a n: 

Standard deviation: 

Number of observ. 

4561/F 4562/F 4563/F 

4/1 4/1 4/1 

290.5 291.0 292.3 285.1 

282.9 

267.5 

297.3 

359.8 

282.6 291.2 294.0 

24.5 

(10) 

294.5 

273.1 

281.8 287.0 287.5 

301.1 338.7 

249.0 262.0 

310.2 

288.5 

25.2 

(10) 

323.0 

306.2 

265.7 

358.1 

288.4 

324.4 

267.1 289.6 

352.2 316.3 309.1 

32.0 

(10) 

302.8 

238.9 265.1 

Sacrifice 

Summary Statistics for % Organ to Brain Weight 

Study number: 006130 

Brain Kidneys 

Adrenal Glands Heart 

2.8865 

4.0617 

3.7428 

2.6745 

3.5053 3.5295 3.5653 

2.9344 

3.3203 3.5115 3.3732 

0.4254 

(10) 

3.9615 

4.7019 4.0809 

2.6718 

3.4819 

3.7101 3.5748 3.6654 3.2956 3.9665 3.7110 

0.5346 

(10) 

4.0973 4.5952 

2.4795 

3.4670 

2. 8713 

3.4021 3.1185 

2.3598 2.3730 

3.4005 3.2164 

0.7397 

(10) 

3.6475 3.7279 3.5484 

F e m a l e 

100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 

100.0000 

100.0000 

100.0000 100.0000 100.0000 

0.0000 

(10) 

100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 

0.0000 

(10) 

100.0000 

100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 

100.0000 100.0000 

0.0000 

(10) 

100.0000 100.0000 100.0000 

A n i m a l s 

50.4200 

50.4919 

49.8158 

46.3249 

55.3669 

47.7755 48.4091 

59.3753 51.2960 

61.1532 

52.0429 

4. 9719 

(10) 

58.7982 51.2596 56.2868 51.5023 

43.7458 

50.5448 56.4309 51.3704 

46.1705 

57.3082 

52.3417 

4.9064 

(10) 

52.3050 57.4280 

46.0509 

63.1852 

49.1850 

51.0317 52.5420 

47.6262 

55.0809 58.1562 53.2591 

5.2692 

(10) 

50.2842 

46.9447 48.4976 

118.7141 104.1353 112.8150 108.5518 125.4184 122.1876 

112.7514 116.7482 118.7198 122.9618 116.3003 

6.7233 

(10) 

137.6965 131.1058 117.6700 101.7078 109.0693 109.9400 138.2140 101.7556 

97.4869 

112.5630 115.7209 

15.0587 

(10) 

149.1773 143.5026 119.5203 131.5805 122.7582 

120.5789 122.6466 110.0794 119.0071 124.1260 126.2977 

11.8921 

(10) 

122.6017 117.2704 119.9359 

Liver 

397.118

412.574

365.651

332.840

437.969433.197404.145

458.429

392.488

460.581409.499

40.353

(10

398.978399.259

410.850

397.382

403.829

381.439

509.639

375.470336.352

436.056404.926

44.989

(10

452.361479.087

369.074

618.937

378.821395.910387.998376.210

461.721444.786436.490

75.690

(10

437.854

397.257

439.163
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal 

Summary Statistics for % Organ to Brain Weight 

Study number: 006130 

Brain Kidneys 

No/sex Subgroup Body wt. (g) Adrenal Glands Heart 

4564/F 4/1 318.2 3.6981 100.0000 53.2335 138.0002 4565/F 4/1 247.0 2.8083 100.0000 44.5496 96.1430 4566/F 4/1 285.9 2.9347 100.0000 46.0347 115.2604 4567/F 4/1 324.7 3.9623 100.0000 52.1896 120.9452 4568/F 4/1 280.1 3. 7728 100.0000 40.4417 114.6380 4569/F 4/1 299.5 2.2629 100.0000 49.6738 115.3839 4570/F 4/1 283.5 3.0966 100.0000 52.7114 103.7775 

M e a n: 284.6 3.3459 100.0000 48.4561 116.3956 

Standard deviation: 28.3 0.5434 0.0000 4.0461 11.1258 

Number of observ. : (10) (10) (10) (10) (10) 

Liver 

454.968

378.362

371.559

532.363

408.633413.239406.426

423.982

46.347

(10
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal 

Summary Statistics for % Organ to Brain Weight 

Study number: 006130 

Spleen Uterus 

No/sex Subgroup Body wt. (g) Ovaries Thymus 

F e m a 1 e A n i m a 1 s 

1561/F 1/1 290.5 5.9818 31.1358 22.0944 38.8790 1562/F 1/1 291.0 5.6558 27.9423 17.7619 66.0809 1563/F 1/1 292.3 4.8234 28.9553 14.5341 40.1788 1564/F 1/1 285.1 4.0021 25.3113 12.4134 55.5889 1565/F 1/1 282.9 4.8916 28.7405 14.5955 45.1579 1566/F 1/1 267.5 4. 7268 26.7612 13.9936 35.2665 1567/F 1/1 297.3 4.6717 31.3548 15.2692 46.0979 

1568/F 1/1 359.8 3.9587 25.2884 14.4136 40.8370 1569/F 1/1 282.6 4.1045 34.8169 18.0323 34.0938 1570/F 1/1 291.2 3.1252 24.6638 14.7650 41.9670 

M e a n: 294.0 4.5942 28.4970 15.7873 44.4148 

Standard deviation: 24.5 0.8412 3.2283 2.7791 9.7483 

Number of observ. : (10) (10) (10) (10) (10) 

2551/F 2/1 294.5 5.7464 30.7650 17.8640 35.1034 2552/F 2/1 273.1 3.9904 24.9231 14.1875 31.4231 2553/F 2/1 281.8 4.0303 22.2840 14.5910 28.1756 2554/F 2/1 287.0 5.5441 28.5036 16.7988 37.9184 2555/F 2/1 287.5 4.4013 26.8839 19.1769 31.2661 2556/F 2/1 301.1 3.9741 24.2765 15.4308 34.9892 2557/F 2/1 338.7 3.2694 29.2841 12.4061 34.6580 2558/F 2/1 249.0 5.2119 27.2647 14.5405 31.7061 2559/F 2/1 262.0 3.9722 18.2454 19.0831 31.8282 

2560/F 2/1 310.2 4.6106 34.3956 17.1348 34.8266 

M e a n: 288.5 4.4751 26.6826 16.1213 33.1895 

Standard deviation: 25.2 0.7969 4.5465 2.2483 2.7911 

Number of observ. : (10) (10) (10) (10) (10) 

3551/F 3/1 323.0 4.9457 31.0120 16.1143 31.6226 3552/F 3/1 306.2 5.6223 28.7671 18.4773 29.6012 3553/F 3/1 265.7 4.2467 23.6182 10.3462 37.6837 3554/F 3/1 358.1 3 .1571 34.7376 16.3471 41.2212 3555/F 3/1 288.4 4.8191 34.7732 17.0359 42.2365 3556/F 3/1 324.4 4.2838 26.5337 21.8622 72.8662 3557/F 3/1 267.1 3.3666 20.9487 15.1739 41.8244 3558/F 3/1 289.6 3.8785 23.6776 14.6355 83.5748 3559/F 3/1 352.2 4.5867 26.9369 15.1218 39.3988 

3560/F 3/1 316.3 4.5995 29.4962 16.5290 57.4710 

M e a n: 309.1 4.3506 28.0501 16.1643 47.7500 

Standard deviation: 32.0 0.7408 4.6431 2.9250 17.8805 

Number of observ. : (10) (10) (10) (10) (10) 

4561/F 4/1 302.8 4.8881 35.8721 17.3924 29.9461 4562/F 4/1 238.9 5.1018 23.8903 19.0094 42.1935 4563/F 4/1 265.1 4.3056 29.0277 12.9023 32.9159 
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Main Study - Terminal Sacrifice 

Animal Group/ Terminal 

Summary Statistics for % Organ to Brain Weight 

Study number: 006130 

Spleen Uterus 

No/sex Subgroup Body wt. (g) Ovaries Thymus 

4564/F 4/1 318.2 5.2200 27.0908 13.9389 27.5650 4565/F 4/1 247.0 3.4682 27.7150 7.2689 39.4803 4566/F 4/1 285.9 4.0334 22.1299 13.1662 30.9199 4567/F 4/1 324.7 3.7001 28.0315 16.6737 42.3259 4568/F 4/1 280.1 3.6774 31.2267 15.5967 41.7724 4569/F 4/1 299.5 4.1973 23.7192 12.8701 27.7339 4570/F 4/1 283.5 4.1851 29.4249 14.6876 71.6586 

M e a n: 284.6 4.2777 27.8128 14.3506 38.6511 

Standard deviation: 28.3 0.6114 4.0235 3.2323 13.0661 

Number of observ. : (10) (10) (10) (10) (10) 
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Terminal Sacrifice 

Animal Group/ Terminal 

No/sex Subgroup Body wt. (g) 

1071/M 1/1 676.4 1072/M 1/1 550.1 1073/M 1/1 557.6 1074/M 1/1 480.0 

1075/M 1/1 528.8 

M e a n: 558.6 

Standard deviation: 72.5 

Number of observ. : (5) 

2061/M 2/1 506.0 

2062/M 2/1 573.5 2063/M 2/1 605.4 2064/M 2/1 472.0 2065/M 2/1 649.1 

M e a n: 561.2 

Standard deviation: 72.2 

Number of observ. : (5) 

3061/M 3/1 619.5 3062/M 3/1 627.3 3063/M 3/1 577.1 3064/M 3/1 597.3 3065/M 3/1 496.6 

M e a n: 583.6 

Standard deviation: 52.4 

Number of observ. : (5) 

4071/M 4/1 516.6 4072/M 4/1 629.8 4073/M 4/1 586.7 4074/M 4/1 452.9 4075/M 4/1 526.3 

M e a n: 542.5 

Standard deviation: 68.1 

Number of observ. : (5) 

Summary statistics for absolute organ weights (g) 

Study number: 006130N 

- Neurotox Brain Measurements 

Length 

weight Width 

M a 1 e A n i m a 1 s 

1. 843 0 2.2 1.5 

1.7890 2.1 1.4 

1.6500 2.1 1.5 1.4600 2.1 1.5 1.6670 2.1 1.5 1.6818 2.1 1.5 

0.1482 0.0 0.0 

(5) (5) (5) 

1.7980 2.1 1.6 

1. 7270 2.1 1.5 

1.7340 2.2 1.5 1.6610 2.2 1.5 

1.8460 2.2 1.5 

1.7532 2.2 1.5 

0. 0710 0.1 0.0 

(5) (5) (5) 

1.9290 2.1 1.5 1.7700 2.2 1.5 1.6880 2.2 1.5 

1.8950 2.2 1.5 

1.6660 2.1 1.5 

1. 7896 2.2 1.5 

0.1189 0.1 0.0 

(5) (5) (5) 

1.7960 2.2 1.5 

1. 8030 2.1 1.5 

1.8350 2.1 1.5 1.6980 2.1 1.5 

1. 6240 2.2 1.5 1. 7512 2.1 1.5 

0.0876 0.1 0.0 

(5) (5) (5) 
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Terminal Sacrifice 

Animal Group/ Terminal 

No/sex Subgroup Body wt. (g) 

1571/F 1/1 316.6 1572/F 1/1 324.2 1573/F 1/1 385.8 1574/F 1/1 348.9 1575/F 1/1 277.1 

M e a n: 330.5 

Standard deviation: 40.3 

Number of observ. : (5) 

2561/F 2/1 287.0 2562/F 2/1 355.8 

2563/F 2/1 318.1 

2564/F 2/1 316.1 2565/F 2/1 315.7 

M e a n: 318.5 

Standard deviation: 24.5 

Number of observ. : (5) 

3561/F 3/1 316.3 3562/F 3/1 259.9 3563/F 3/1 269.7 3564/F 3/1 344.7 3565/F 3/1 388.9 

M e a n: 315.9 

Standard deviation: 53.5 

Number of observ. : (5) 

4571/F 4/1 364.3 

4572/F 4/1 309.9 

4573/F 4/1 307.8 4574/F 4/1 283.5 4575/F 4/1 318.2 

M e a n: 316.7 

Standard deviation: 29.6 

Number of observ. : (5) 

Summary statistics for absolute organ weights (g) 

Study number: 006130N 

- Neurotox Brain Measurements 

Length 

Weight Width 

F e m a 1 e A n i m a 1 s 

1.6420 2.1 1.5 1.5960 2.1 1.5 

l. 6540 2.1 1.5 

1.6850 2.1 1.5 1.6700 2.1 1.4 

l. 6494 2.1 1.5 

0.0340 0.0 0.0 

(5) (5) (5) 

1. 6720 2.1 1.5 

1.7490 2.1 1.5 1.6750 2.1 1.5 1.6990 2.1 1.5 

l. 7040 2.1 1.5 

1.6998 2.1 1.5 

0.0309 0.0 0.0 

(5) (5) (5) 

1.6420 2.1 1.4 

l. 9130 2.1 1.5 l. 5010 2.1 1.3 

1.6980 2.1 1.5 1.6390 2.2 1.5 1.6786 2.1 1.4 

0.1498 0.0 0.1 

(5) (5) (5) 

1.7410 2.1 1.5 

1. 7150 2.2 1.4 

1.5890 2.1 1.4 1.7620 2.1 1.4 

1. 7220 2.1 1.4 

1.7058 2.1 1.4 

0.0678 0.0 0.0 

(5) (5) (5) 
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Recovery Sacrifice 

Animal Group/ Terminal 

No/sex Subgroup Body wt. (g) 

1051/M 1052/M 1053/M 1054/M 1055/M 

1056/M 

1057/M 1058/M 1059/M 

1060/M 

1/1 1/1 1/1 1/1 

1/1 1/1 

1/1 1/1 1/1 

1/1 

M e a n: 

Standard deviation: 

Number of observ. 

4051/M 4/1 4052/M 4/1 4053/M 4/1 4054/M 4/1 4055/M 4/1 4056/M 4/1 4057/M 4/1 4058/M 4/1 4059/M 4/1 

4060/M 4/1 

M e a n: 

Standard deviation: 

Number of observ. : 

559.1 523.7 559.3 509.5 516.7 587.2 503.5 

608.6 

535.9 501.9 

540.5 

36.8 

(10) 

568.5 532.4 565.0 533.3 

488.0 

520.2 566.7 554.5 520.7 

586.9 

543.6 

29.8 

(10) 

Summary statistics for absolute organ weights (g) 

Study number: 006130 

Adrenal Glands 

0.0603 0.0798 0.0599 

0.0793 

0.0549 0.0637 0.0855 0.0763 0.0598 

0.0660 0.0686 0.0107 

(10) 

0.0621 0.0665 0.0631 0.0700 0.0904 0.0601 0.0753 0.0628 0.0696 

0. 0710 0.0691 0.0089 

(10) 

Brain 

M a 1 e 

2.2150 

2 .1165 

2.2935 2.0569 2.2336 2.3081 2.3388 2.3123 2.3616 2.1799 

2.2416 

0.1002 

(10) 

2.1738 

2 .1162 

2.3360 2.3030 2.1655 2.3611 2.3106 2.1846 2.3381 

2.3435 2.2632 

0.0920 

(10) 

Epididymis 

A n i m a 1 s 

1.5185 1.7080 

1. 4124 

1.5351 

1. 5524 1. 5194 

1.5322 

1.5963 

1.5890 1.5801 

1.5543 

0.0751 

(10) 

1.6130 1.6675 1.7339 1.7621 1.7910 1.3080 

1. 5882 

1.6300 1.6856 

1.5781 1.6357 

0.1361 

(10) 

Heart 

1.9896 

1.5497 

1.5605 

1. 5344 

1.5558 1.8500 

1. 6429 

1.8142 1.9068 1.5850 

1.6989 

0.1729 

(10) 

1.7857 1.7788 

1. 8936 

1.7733 

1. 5879 1. 5963 1. 9201 

1.4654 

1. 7011 

1.7816 1.7284 

0.1421 

(10) 

Kidneys 

4.411

4. 091

3.8193.741

4.657

5 .115

4.1794.8534.6634.089

4.362

0.453

(10

4.341

3.938

4.0914.7854.1694.534

5.095

4.425

5.415

5.464

4.626

0.544

(10
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Recovery Sacrifice 

Animal Group/ Terminal 

No/sex Subgroup Body wt. (g) 

1051/M 1052/M 

1053/M 1054/M 

1055/M 1056/M 1057/M 1058/M 1059/M 1060/M 

1/1 

1/1 

1/1 1/1 1/1 1/1 1/1 1/1 1/1 

1/1 

M e a n: 

Standard deviation: 

Number of observ. 

4051/M 4/1 4052/M 4/1 4053/M 4/1 4054/M 4/1 4055/M 4/1 4056/M 4/1 4057/M 4/1 4058/M 4/1 4059/M 4/1 4060/M 4/1 

M e a n: 

Standard deviation: 

Number of observ. : 

559.1 523.7 559.3 509.5 

516.7 

587.2 503.5 

608.6 

535.9 501.9 540.5 

36.8 

(10) 

568.5 532.4 565.0 533.3 

488.0 

520.2 566.7 554.5 520.7 586.9 543.6 

29.8 

(10) 

Summary statistics for absolute organ weights (g) 

Study number: 006130 

Prostate Spleen 

Lungs Seminal vesicles 

2.2079 

1.8610 1.9705 

2.5807 

1.8505 1.9294 

2.1541 

1. 8028 

2.1229 

1. 7517 

2.0232 

0.2499 

(10) 

1.9827 

2.2708 2.3857 2.2257 2.0069 

1.9762 

2.2217 

1.8333 

2.0006 2.0785 2.0982 

0.1702 

(10) 

M a 1 e 

1.1263 

1.3119 

0.7913 

1.3059 

1. 6364 1. 2344 

1.7102 

1. 2673 

1.0652 

1.3387 

1.2788 

0.2642 

(10) 

1. 0703 

1.1222 

1.1576 

1. 2516 

1.2977 

0.9984 

1.2537 

0.9621 

1. 6024 

1.1271 

1.1843 

0.1831 

(10) 

A n i m a 1 s 

1.9863 1.5410 1.5819 

0.6735 

1.9735 

2.8140 

1.9832 

2.3253 

1.9398 1.6169 1.8435 

0.5598 

(10) 

2.7714 2.1199 

1.6621 1.4088 

2.5209 

1. 5904 

2.9253 2.5867 2.2400 2.0801 2.1906 

0.5179 

(10) 

1.0800 

0.9046 0.8309 0.9196 0.8330 0.9879 0.5653 0.9743 0.8730 0. 7183 0.8687 

0.1458 

(10) 

0.8881 

0.9269 

1.0225 

0.7904 

0.6555 

0.9293 

1. 0519 

0.8267 0.5998 

1. 2107 

0.8902 0.1834 

(10) 

Testes 

3.109

3.282

3.317

3.2313.4523.496

3.2163.8203.7503.5273.420

0.233

(10

3.747

4.151

3.8333.7623.751

3.087

4.050

3.6423.754

3. 639

3.742

0.282

(10
Huntingdon Life Sciences Summary statistics for absolute organ weights (g) 

Princeton Research Center Study number: 006130 

East Millstone, New Jersey 

AJ2J2endix L: Recovery Sacrifice 

Animal Group/ Terminal Brain Kidneys 

No/sex Subgroup Body wt. (g) Adrenal Glands Heart Live

-----------------------------------------------------------------------------------------------------------------

Female A n i m a 1 s 

1551/F 1/1 306.0 0.0813 2.1871 1.1239 2.5152 9.4061552/F 1/1 294.8 0.0650 2.0815 0.9216 2.1260 7.9721553/F 1/1 275.1 0.0949 2.2919 1.0433 2.3420 9.5091554/F 1/1 335.5 0.0790 2.0611 1.0610 2.6179 8.9251555/F 1/1 307.1 0.0653 2.2349 1.0452 2.4540 8.6821556/F 1/1 280.2 0.0663 2 .1128 1. 0629 2.4021 8.7231557/F 1/1 262.2 0.0668 2.0631 1.0810 2.4876 8.1241558/F 1/1 285.2 0.0666 2.1593 0.9973 2.4896 8.2171559/F 1/1 273.6 0.0758 2.1249 0. 9972 2.2122 9.2951560/F 1/1 269.4 0.0845 2.1035 0.9490 2.3145 9.419

M e a n: 288.9 0.0746 2.1420 1.0282 2.3961 8.827

Standard deviation: 22.2 0.0103 0.0764 0.0617 0.1492 0.578

Number of observ. : (10) (10) (10) (10) (10) (10

4551/F 4/1 287.6 0. 0777 2.0266 1. 0127 2.2872 7.9574552/F 4/1 274.9 0.0799 2.1472 1.1731 2.4381 8.4024553/F 4/1 314.7 0.0837 2. 0717 1. 0554 2. 7286 9.6214554/F 4/1 305.7 0.0731 2.0974 1. 0770 2.3284 8.6014555/F 4/1 295.9 0.0864 2.1098 1. 0643 2.4985 9.495

4556/F 4/1 297.9 0.0796 2.1741 2.0578 2.2036 11.1634557/F 4/1 279.1 0.0762 2.1646 1.0018 2.5828 9. 4674558/F 4/1 305.0 0.0868 2.0486 1.0408 2.3864 8.2154559/F 4/1 266.8 0.0657 2.0838 1.0447 2.2137 6.6334560/F 4/1 317.5 0.0740 2.0574 1.2935 2. 7113 10.029

M e a n: 294.5 0.0783 2.0981 1.1821 2.4379 8.958

Standard deviation: 17.1 0.0065 0.0504 0.3198 0.1904 1.264

Number of observ. : (10) (10) (10) (10) (10) (10
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Recovery Sacrifice 

Animal Group/ Terminal 

No/sex Subgroup Body wt. (g) 

1551/F 1/1 306.0 1552/F 1/1 294.8 1553/F 1/1 275.1 

1554/F 1/1 335.5 

1555/F 1/1 307.1 1556/F 1/1 280.2 1557/F 1/1 262.2 1558/F 1/1 285.2 

1559/F 1/1 273.6 

1560/F 1/1 269.4 

M e a n: 288.9 

Standard deviation: 22.2 

Number of observ. : (10) 

4551/F 4/1 287.6 4552/F 4/1 274.9 4553/F 4/1 314.7 4554/F 4/1 305.7 4555/F 4/1 295.9 4556/F 4/1 297.9 4557/F 4/1 279.1 4558/F 4/1 305.0 4559/F 4/1 266.8 4560/F 4/1 317.5 

M e a n: 294.5 

Standard deviation: 17.1 

Number of observ. : (10) 

Summary statistics for absolute organ weights (g) 

Study number: 006130 

Spleen Uterus 

Ovaries Thymus 

F e m a 1 e A n i m a 1 s 

0.0570 0.5520 0. 2672 1.0315 0.0896 0.5513 0.3332 1.2383 0.1128 0.5279 0.2937 0.7314 0.1128 0.7643 0.3016 0.6593 

0.0598 0.6882 0.1341 1.2982 

0.1020 0.5478 0.2870 0.9852 0.0609 0.5547 0.2230 0.8357 

0.0895 0.5931 0.1999 1. 5834 

0.1058 0.6044 0.2773 0.7876 0.1302 0.6469 0.2263 0. 7972 0. 0920 0.6031 0.2543 0.9948 

0.0255 0.0757 0.0586 0.2960 

(10) (10) (10) (10) 

0.1018 0.5495 0.2523 0.6932 

0.0942 0.5164 0.1902 0.5793 

0.1512 0.6332 0.2802 1.4908 

0.1087 0.7670 0.3150 0.7855 0.0797 0. 5096 0.2652 1.1993 0.0800 0.7456 0.3026 0.7581 0.1153 0.5887 0.2361 0.5021 0.0846 0.5272 0.3122 0.5336 

0.0631 0.5532 0.2726 0.9410 

0.0926 0.5965 0.2288 0.7160 0. 0971 0.5987 0.2655 0.8199 0.0244 0.0916 0.0399 0.3129 

(10) (10) (10) (10) 
Huntingdon Life Sciences Summary Statistics for % Organ to Body Weight 

Princeton Research Center Study number: 006130 

East Millstone, New Jersey 

AJ2J2endix L: Recovery Sacrifice 

Animal Group/ Terminal Brain Heart 

No/sex Subgroup Body wt. (g) Adrenal Glands Epididymis Kidneys 

-----------------------------------------------------------------------------------------------------------------

M a 1 e A n i m a 1 s 

1051/M 1/1 559.1 0.0108 0.3962 0. 2716 0.3559 0.7891052/M 1/1 523.7 0.0152 0.4041 0.3261 0.2959 0.7811053/M 1/1 559.3 0.0107 0.4101 0.2525 0.2790 0.6821054/M 1/1 509.5 0.0156 0. 4037 0.3013 0.3012 0.7341055/M 1/1 516.7 0.0106 0.4323 0.3004 0.3011 0.9011056/M 1/1 587.2 0.0108 0.3931 0.2588 0.3151 0.8711057/M 1/1 503.5 0.0170 0.4645 0.3043 0.3263 0.8301058/M 1/1 608.6 0.0125 0.3799 0.2623 0.2981 0.7971059/M 1/1 535.9 0.0112 0.4407 0.2965 0.3558 0.8701060/M 1/1 501.9 0.0132 0.4343 0.3148 0.3158 0.814

M e a n: 540.5 0.0128 0.4159 0.2889 0. 3144 0.807

Standard deviation: 36.8 0.0024 0.0261 0.0256 0.0254 0.066

Number of observ. : (10) (10) (10) (10) (10) (10

4051/M 4/1 568.5 0.0109 0.3824 0.2837 0.3141 0.763

4052/M 4/1 532.4 0.0125 0.3975 0.3132 0.3341 0.7394053/M 4/1 565.0 0.0112 0.4135 0.3069 0.3352 0.7244054/M 4/1 533.3 0.0131 0.4318 0.3304 0.3325 0.8974055/M 4/1 488.0 0.0185 0.4438 0.3670 0.3254 0.8544056/M 4/1 520.2 0. 0116 0.4539 0.2514 0.3069 0. 8714057/M 4/1 566.7 0.0133 0.4077 0.2803 0.3388 0.8994058/M 4/1 554.5 0. 0113 0.3940 0.2940 0.2643 0.7984059/M 4/1 520.7 0.0134 0.4490 0.3237 0.3267 1. 0404060/M 4/1 586.9 0.0121 0.3993 0.2689 0.3036 0. 931

M e a n: 543.6 0.0128 0.4173 0.3020 0.3181 0.852

Standard deviation: 29.8 0.0022 0.0255 0.0336 0.0225 0.097

Number of observ. : (10) (10) (10) (10) (10) (10
Huntingdon Life Sciences Summary Statistics for % Organ to Body Weight 

Princeton Research Center Study number: 006130 

East Millstone, New Jersey 

AEEendix L: Recovery Sacrifice 

Animal Group/ Terminal Prostate Spleen 

No/sex Subgroup Body wt. (g) Lungs Seminal vesicles Testes 

-----------------------------------------------------------------------------------------------------------------

M a 1 e A n i m a 1 s 

1051/M 1/1 559.1 0.3949 0.2014 0.3553 0.1932 0.5561052/M 1/1 523.7 0.3554 0.2505 0.2943 0 .1727 0.6261053/M 1/1 559.3 0.3523 0.1415 0.2828 0.1486 0.5931054/M 1/1 509.5 0.5065 0.2563 0.1322 0.1805 0.6341055/M 1/1 516.7 0.3581 0.3167 0.3819 0.1612 0.6681056/M 1/1 587.2 0.3286 0.2102 0.4792 0.1682 0.5951057/M 1/1 503.5 0.4278 0.3397 0.3939 0.1123 0.6381058/M 1/1 608.6 0.2962 0.2082 0.3821 0.1601 0.6271059/M 1/1 535.9 0.3961 0.1988 0.3620 0.1629 0.6991060/M 1/1 501.9 0.3490 0.2667 0.3222 0.1431 0.702

M e a n: 540.5 0.3765 0.2390 0.3386 0.1603 0.634

Standard deviation: 36.8 0.0588 0.0593 0.0915 0.0222 0.046

Number of observ. (10) (10) (10) (10) (10) (10

4051/M 4/1 568.5 0.3488 0.1883 0.4875 0.1562 0.6594052/M 4/1 532.4 0.4265 0.2108 0.3982 0.1741 0.7794053/M 4/1 565.0 0.4222 0.2049 0.2942 0.1810 0.6784054/M 4/1 533.3 0.4173 0.2347 0.2642 0.1482 0.7054055/M 4/1 488.0 0.4113 0.2659 0.5166 0.1343 0.7684056/M 4/1 520.2 0.3799 0.1919 0.3057 0.1786 0.5934057/M 4/1 566.7 0.3920 0.2212 0.5162 0.1856 0. 7144058/M 4/1 554.5 0.3306 0.1735 0.4665 0.1491 0.656

4059/M 4/1 520.7 0.3842 0.3077 0.4302 0 .1152 0.7214060/M 4/1 586.9 0.3541 0.1920 0.3544 0.2063 0.620

M e a n: 543.6 0.3867 0.2191 0.4034 0.1629 0.689

Standard deviation: 29.8 0.0335 0.0409 0.0946 0.0272 0.060

Number of observ. : (10) (10) (10) (10) (10) (10
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Recovery Sacrifice 

Animal Group/ Terminal 

No/sex Subgroup Body wt. (g) 

1551/F 1/1 1552/F 1/1 1553/F 1/1 1554/F 1/1 1555/F 1/1 1556/F 1/1 1557/F 1/1 1558/F 1/1 1559/F 1/1 1560/F 1/1 

M e a n: 

Standard deviation: 

Number of observ. 

4551/F 4/1 4552/F 4/1 4553/F 4/1 4554/F 4/1 4555/F 4/1 4556/F 4/1 4557/F 4/1 4558/F 4/1 4559/F 4/1 

4560/F 4/1 

M e a n: 

Standard deviation: 

Number of observ. : 

306.0 

294.8 275.1 

335.5 307.1 

280.2 262.2 285.2 273.6 269.4 

288.9 

22.2 

(10) 

287.6 274.9 

314.7 305.7 

295.9 297.9 279.1 

305.0 

266.8 

317.5 

294.5 

17.1 

(10) 

Summary Statistics for % Organ to Body Weight 

Study number: 006130 

Adrenal Glands 

0.0266 0.0220 0.0345 0.0235 0.0213 0.0237 0.0255 0.0234 0.0277 0.0314 

0.0259 0.0042 

(10) 

0.0270 0.0291 

0.0266 0.0239 0.0292 0.0267 

0.0273 0.0285 0.0246 

0.0233 0.0266 0.0021 

(10) 

Brain 

F e m a l e 

0. 7147 0.7061 0.8331 0.6143 0.7277 0.7540 0.7868 0.7571 0.7766 0. 7808 

0.7451 0.0595 

(10) 

0.7047 0. 7811 0.6583 0.6861 0.7130 0.7298 

0.7756 0.6717 0.7810 

0.6480 

0.7149 0.0507 

(10) 

Heart 

A n i m a 1 s 

0.3673 0.3126 0.3792 0.3162 0.3403 0.3793 0.4123 0.3497 0.3645 0.3523 

0.3574 0.0302 

(10) 

0.3521 

0.4267 

0.3354 0.3523 

0.3597 0.6908 0.3589 0.3412 0.3916 

0.4074 

0.4016 0.1059 

(10) 

Kidneys 

0.8220 0. 7212 0.8513 0.7803 0.7991 0.8573 0.9487 0. 8729 0.8086 0.8591 

0.8320 0.0612 

(10) 

0.7953 0.8869 0.8670 0.7617 0.8444 0.7397 0.9254 0.7824 0.8297 

0.8540 

0.8286 0.0585 

(10) 

Liver 

3.073

2.704

3.456

2.660

2.827

3 .113

3.098

2. 881

3.397

3.496

3. 071

0.305

(10

2.766

3.0563.057

2.813

3.2093.7473.392

2.6932.486

3.1583.038

0.368

(10
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Recovery Sacrifice 

Animal Group/ Terminal 

No/sex Subgroup Body wt. (g) 

1551/F 1/1 306.0 1552/F 1/1 294.8 1553/F 1/1 275.1 

1554/F 1/1 335.5 

1555/F 1/1 307.1 1556/F 1/1 280.2 1557/F 1/1 262.2 1558/F 1/1 285.2 1559/F 1/1 273.6 1560/F 1/1 269.4 

M e a n: 288.9 

Standard deviation: 22.2 

Number of observ. : (10) 

4551/F 4/1 287.6 4552/F 4/1 274.9 

4553/F 4/1 314.7 4554/F 4/1 305.7 4555/F 4/1 295.9 4556/F 4/1 297.9 4557/F 4/1 279.1 4558/F 4/1 305.0 4559/F 4/1 266.8 

4560/F 4/1 317.5 

M e a n: 294.5 

Standard deviation: 17.1 

Number of observ. : (10) 

Summary Statistics for % Organ to Body Weight 

Study number: 006130 

Spleen Uterus 

Ovaries Thymus 

F e m a 1 e A n i m a 1 s 

0.0186 0.1804 0.0873 0.3371 

0.0304 0.1870 0.1130 0.4200 

0.0410 0.1919 0.1068 0.2659 

0.0336 0.2278 0.0899 0.1965 

0.0195 0.2241 0.0437 0.4227 

0.0364 0.1955 0.1024 0.3516 

0.0232 0.2116 0.0850 0.3187 0.0314 0.2080 0.0701 0.5552 0.0387 0.2209 0.1014 0.2879 

0.0483 0.2401 0.0840 0.2959 

0.0321 0.2087 0.0884 0.3452 0. 0096 0.0197 0.0202 0.1004 

(10) (10) (10) (10) 

0.0354 0.1911 0.0877 0.2410 

0.0343 0.1879 0.0692 0.2107 

0.0480 0.2012 0.0890 0.4737 

0.0356 0.2509 0.1030 0.2570 0.0269 0.1722 0.0896 0.4053 

0.0269 0.2503 0.1016 0.2545 

0.0413 0.2109 0.0846 0.1799 

0.0277 0.1729 0.1024 0.1750 

0.0237 0.2073 0.1022 0.3527 

0.0292 0.1879 0. 0721 0.2255 0.0329 0.2033 0.0901 0.2775 0.0076 0.0280 0.0125 0.1000 

(10) (10) (10) (10) 
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Sacrifice 

Animal Group/ Terminal 

No/sex Subgroup Body wt. (g) 

1051/M 1/1 1052/M 1/1 1053/M 1/1 

1054/M 1/1 1055/M 1/1 1056/M 1/1 1057/M 1/1 1058/M 1/1 1059/M 1/1 1060/M 1/1 

M e a n: 

Standard deviation: 

Number of observ. 

4051/M 4/1 4052/M 4/1 

4053/M 4/1 

4054/M 4/1 4055/M 4/1 4056/M 4/1 4057/M 4/1 4058/M 4/1 4059/M 4/1 

4060/M 4/1 

M e a n: 

Standard deviation: 

Number of observ. : 

559.1 523.7 559.3 

509.5 516.7 587.2 503.5 

608.6 

535.9 501.9 

540.5 

36.8 

(10) 

568.5 532.4 

565.0 533.3 

488.0 

520.2 566.7 

554.5 

520.7 586.9 543.6 

29.8 

(10) 

Summary Statistics for % Organ to Brain Weight 

Study number: 006130 

Adrenal Glands 

2.7223 

3.7704 

2.6117 

3.8553 

2.4579 

2.7598 

3.6557 3.2997 

2.5322 

3.0277 

3.0693 

0. 5371 

(10) 

2.8567 

3.1424 

2.7012 

3.0395 

4.1746 

2.5454 

3.2589 

2.8747 2.9768 

3.0297 3.0600 

0.4430 

(10) 

Brain 

M a 1 e 

100.0000 100.0000 100.0000 

100.0000 100.0000 100.0000 

100.0000 100.0000 100.0000 100.0000 

100.0000 

0.0000 

(10) 

100.0000 100.0000 

100.0000 100.0000 100.0000 

100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 

0.0000 

(10) 

Epididymis 

A n i m a 1 s 

68.5553 

80.6993 

61.5827 

74.6317 

69.5022 65.8290 65.5122 69.0352 67.2849 

72 .4850 

69.5118 

5.3618 

(10) 

74.2019 78.7969 

74.2252 

76.5133 

82.7061 

55.3979 

68.7354 

74.6132 

72.0927 

67.3395 

72.4622 

7.4811 

(10) 

Heart 

89.8239 

73.2199 

68.0401 

74.5977 

69.6544 

80.1525 

70.2454 78.4587 

80.7419 

72.7098 

75.7644 

6.6130 

(10) 

82.1465 84.0563 

81.0616 

76.9996 73.3272 

67.6083 

83.0996 

67.0786 

72.7557 

76.0230 

76.4156 

6.1973 

(10) 

Kidneys 

199.178193.328

166.531

181.904

208.528221.632

178.707

209.916

197.467187.586

194.478

16.468

(10

199.737186.116

175.167

207.811

192.560192.037

220.522202.590231.602233.176204.132

19.281

(10
Huntingdon Life Sciences Summary Statistics for % Organ to Brain Weight 

Princeton Research Center Study number: 006130 

East Millstone, New Jersey 

A22endix L: Recovery Sacrifice 

Animal Group/ Terminal Prostate Spleen 

No/sex Subgroup Body wt. (g) Lungs Seminal vesicles Testes 

-----------------------------------------------------------------------------------------------------------------

M a 1 e A n i m a 1 s 

1051/M 1/1 559.1 99.6795 50.8488 89.6749 48.7585 140.3881052/M 1/1 523.7 87.9282 61.9844 72.8089 42.7404 155.0951053/M 1/1 559.3 85.9167 34.5019 68.9732 36.2285 144.652

1054/M 1/1 509.5 125.4655 63.4887 32.7435 44.7081 157.0811055/M 1/1 516.7 82.8483 73.2629 88.3551 37.2941 154.5891056/M 1/1 587.2 83.5926 53.4812 121.9185 42.8014 151.4961057/M 1/1 503.5 92.1028 73.1230 84.7956 24.1705 137.5191058/M 1/1 608.6 77.9657 54.8069 100.5622 42.1355 165.203

1059/M 1/1 535.9 89.8925 45.1050 82.1392 36.9665 158.8071060/M 1/1 501.9 80.3569 61.4111 74.1731 32.9511 161.819

M e a n: 540.5 90.5749 57.2014 81.6144 38.8754 152.665

Standard deviation: 36.8 13.7582 12.0790 23.0587 6.9694 9.150

Number of observ. : (10) (10) (10) (10) (10) (10

4051/M 4/1 568.5 91.2089 49.2364 127.4910 40.8547 172.3984052/M 4/1 532.4 107.3056 53.0290 100.1749 43.8002 196.186

4053/M 4/1 565.0 102.1276 49.5548 71.1515 43.7714 164.1224054/M 4/1 533.3 96.6435 54.3465 61.1724 34.3205 163.3564055/M 4/1 488.0 92.6761 59.9261 116.4119 30.2701 173.2164056/M 4/1 520.2 83.6983 42.2854 67.3584 39.3588 130.7824057/M 4/1 566.7 96.1525 54.2586 126.6035 45.5250 175.2964058/M 4/1 554.5 83.9193 44.0401 118.4061 37.8422 166.7354059/M 4/1 520.7 85.5652 68.5343 95.8043 25.6533 160.570

4060/M 4/1 586.9 88.6921 48.0947 88.7604 51.6621 155.306

M e a n: 543.6 92.7989 52.3306 97.3335 39.3058 165.797

Standard deviation: 29.8 7.8662 7.6983 24.8323 7.6661 16.618

Number of observ. : (10) (10) (10) (10) (10) (10
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Recovery Sacrifice 

Animal Group/ Terminal 

No/sex Subgroup Body wt. (g) 

1551/F 1/1 1552/F 1/1 1553/F 1/1 

1554/F 1/1 1555/F 1/1 

1556/F 1/1 1557/F 1/1 1558/F 1/1 1559/F 1/1 1560/F 1/1 

M e a n: 

Standard deviation: 

Number of observ. 

4551/F 4/1 4552/F 4/1 4553/F 4/1 4554/F 4/1 4555/F 4/1 4556/F 4/1 4557/F 4/1 4558/F 4/1 4559/F 4/1 

4560/F 4/1 

M e a n: 

Standard deviation: 

Number of observ. : 

306.0 

294.8 275.1 

335.5 

307.1 

280.2 262.2 285.2 273.6 269.4 

288.9 

22.2 

(10) 

287.6 

274.9 

314.7 

305.7 

295.9 297.9 279.1 

305.0 

266.8 

317.5 

294.5 

17.1 

(10) 

Summary Statistics for % Organ to Brain Weight 

Study number: 006130 

Adrenal Glands 

3. 7173 

3.1227 

4.1407 

3.8329 

2.9218 

3.1380 3.2378 3.0843 3.5672 

4.0171 

3.4780 

0.4327 

(10) 

3.8340 

3. 7211 

4.0402 

3.4853 

4.0952 

3.6613 3.5203 

4.2370 

3.1529 

3.5968 3.7344 

0.3260 

(10) 

Brain 

Female 

100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 

100.0000 

0.0000 

(10) 

100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 

100.0000 

100.0000 

0.0000 

(10) 

Heart 

A n i m a 1 s 

51.3877 

44.2758 45.5212 

51.4774 

46.7672 

50.3077 52.3969 

46.1863 

46.9293 

45 .1153 

48.0365 

3.0277 

(10) 

49.9704 

54.6339 50.9437 51.3493 50.4455 

94.6507 

46.2811 

50.8054 50.1344 

62.8706 

56.2085 

14.1922 

(10) 

Kidneys 

115.0016 102.1379 102.1860 127.0147 109.8036 113.6927 120.5758 115.2966 104.1084 110.0309 

111.9848 

8. 0711 

(10) 

112.8590 113.5479 131.7083 111.0136 118.4236 101.3569 119.3200 116.4893 106.2338 

131.7828 

116.2735 

9.7910 

(10) 

Live

430.06

383.03

414.904433.06

388.50

412.88

393.810380.54

437.465

447.810

412.20

24.54

(1

392.66391.30

464.41

410.11

450.06

513.45

437.36401.02

318.32

487.46426.61

56.16

(1
Huntingdon Life Sciences 

Princeton Research Center 

East Millstone, New Jersey 

Appendix L: Recovery Sacrifice 

Animal Group/ Terminal 

No/sex Subgroup Body wt. (g) 

1551/F 1/1 306.0 1552/F 1/1 294.8 1553/F 1/1 275.1 1554/F 1/1 335.5 1555/F 1/1 307.1 1556/F 1/1 280.2 1557/F 1/1 262.2 1558/F 1/1 285.2 

1559/F 1/1 273.6 1560/F 1/1 269.4 

M e a n: 288.9 

Standard deviation: 22.2 

Number of observ. : (10) 

4551/F 4/1 287.6 4552/F 4/1 274.9 4553/F 4/1 314.7 4554/F 4/1 305.7 4555/F 4/1 295.9 4556/F 4/1 297.9 4557/F 4/1 279.1 4558/F 4/1 305.0 4559/F 4/1 266.8 

4560/F 4/1 317.5 

M e a n: 294.5 

Standard deviation: 17.1 

Number of observ. : (10) 

Summary Statistics for % Organ to Brain Weight 

Study number: 006130 

Spleen Uterus 

Ovaries Thymus 

F e m a l e A n i m a l s 

2.6062 25.2389 12.2171 47.1629 

4.3046 26.4857 16.0077 59.4908 

4.9217 23.0333 12.8147 31.9124 

5.4728 37.0821 14.6330 31.9878 

2.6757 30.7933 6.0003 58.0876 

4.8277 25.9277 13.5839 46.6301 

2.9519 26.8867 10.8090 40.5070 

4.1449 27.4672 9.2576 73.3293 4.9791 28.4437 13.0500 37.0653 

6.1897 30.7535 10.7583 37.8987 

4.3074 28.2112 11.9131 46.4072 

1. 2211 3.9161 2.8620 13.5217 

(10) (10) (10) (10) 

5.0232 27.1144 12.4494 34.2051 

4.3871 24.0499 8.8580 26.9793 

7.2984 30.5643 13.5251 71.9602 

5.1826 36.5691 15.0186 37.4511 

3.7776 24.1539 12.5699 56.8443 

3.6797 34.2947 13.9184 34.8696 

5.3266 27.1967 10.9073 23.1960 

4.1297 25.7346 15.2397 26.0471 

3.0281 26.5477 13.0819 45.1579 

4.5008 28.9929 11.1208 34.8012 4.6334 28.5218 12.6689 39.1512 

1.1826 4.1761 1.9629 15.1460 

(10) (10) (10) (10) 
Huntingdon Life Sciences 00-6130 Page 630

211-DIPE-S Final Report

Individual Animal Gross and

Microscopic Observations

Preface Appendix M

Key to Abbreviations

Fibr = Fiber

Gang = Ganglia

LN = Lymph Node

Nose/Turb Sec 1 = Nose/Turbinates Section 1

Nose/Turb Sec 2 = Nose/Turbinates Section 2

Nose/Turb Sec 3 = Nose/Turbinates Section 3

Nose/Turb Sec 4 = Nose/Turbinates Section 4

SC = Spinal Cord

Submandib/Max = Submandibular/Submaxillary

Ventrl = Ventral

V-DVTC = Ventral Diverticulum

V-SM-G = Ventral Seromucinous Glands

Notes:

1. The exposure levels for each group were as follows:

Group 1 – 0 mg/m3

Group 2 – 2000 mg/m3

Group 3 – 10000 mg/m3

Group 4 – 20000 mg/m3

2. Unless otherwise specified in a histopathology note, the organ/tissue 

examined was the required (routine) section.

3. “Phase Day of Death” is calculated from the first day of the indicated phase.

Table of Contents:

Individual Animal Gross and Microscopic Observations (Main and Recovery) . 631

Individual Animal Gross and Microscopic Observations (Neuropathology) ...... 775
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

Thymus 

Discolored, Red, Foci, </= 0.1 em, Slight 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Examined; 1 correlation found: 

CONGESTION, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue 

Thymus 

Gross Observations/Comments 

Discolored, Right lobe, Red, Foci, </= 0.1 

em, Slight 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Examined; 1 correlation found: 

CONGESTION, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue 

Lungs ........ . 

Gross Observations/Comments 

Discolored, All lobes, Red, Foci, </= 0.1 

em, Slight 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissue is unremarkable. 

Examined; no correlation found 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Esophagus No gross observations on tissue. 

Heart . No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is missing. 

A

MONONUCLEAR CELL INFILTRATE WITH MYOFIBER 

DEGENERATION/LOSS, Minimal. 

Liver ......... No gross observations on tissue. 

Mediastinal LN .... No gross observations on tissue. 

Lungs . . No gross observations on tissue. 

Larynx: V-SM-G . No gross observations on tissue. 

Thyroid gland ..... No gross observations on tissue. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

SINUSES: FREE ERYTHROCYTES, Minimal. 

Tissue is unremarkable (with comment) . 

- One lobe is atelectatic. 

Tissue is unremarkable (with comment). 

- Ventral seromucous glands are absent. 

Tissue is unremarkable (with comment). 

- Kursteiner's cysts are present 

bilaterally. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain Sternum Femur 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Cecum Colon Duodenum 

Jejunum 

Sciatic Nerve 

Pancreas 

Thoracic SC 

Seminal vesicles 

Kidneys 

Nose/Turb Sec 1 

Prostate 

Lumbar SC 

Testes 

Mesenteric LN 

Nose/Turb Sec 2 

Parathyroid 

Salivary glands 

Thymus 

Epididymis 

Submandib/Max LN 

Nose/Turb Sec 3 

Rectum/Low Colon 

Spleen 

Trachea 

Ileum 

Larynx: V-DVTC 

Nose/Turb Sec 4 

Cervical SC 

Stomach 

Urinary bladder 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

SEX: Male 

PHASE: Dosing phase 

A

Tissue Gross Observations/Comments 

Heart . . No gross observations on tissue. 

Kidneys ........ No gross observations on tissue. 

Liver . . No gross observations on tissue. 

Mediastinal LN .... No gross observations on tissue. 

Lungs ........ . 

Larynx: V-DVTC 

Discolored, All lobes, Red, Foci, </= 0.1 

em, Slight 

No gross observations on tissue. 

Larynx: V-SM-G .... No gross observations on tissue. 

Parathyroid ...... No gross observations on tissue. 

Microscopic Observations/Comments 

MONONUCLEAR CELL INFILTRATE WITH MYOFIBER 

DEGENERATION/LOSS, Minimal. 

BASOPHILIC TUBULES, Minimal. 

CORTEX: LYMPHOID CELL AGGREGATE(S), 

Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

SINUSES: FREE ERYTHROCYTES, Minimal. 

Tissue is unremarkable. 

Examined; no correlation found 

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

Tissue is unremarkable (with comment) . 

- Ventral seromucous glands are absent. 

Tissue is unremarkable; one-of-pair 

missing. 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum Colon Duodenum 

Ileum Jejunum Mesenteric LN 

Nose/Turb Sec 1 Nose/Turb Sec 2 Nose/Turb Sec 3 

Prostate Rectum/Low Colon Cervical SC 

Salivary glands Spleen Stomach 

Thyroid gland Thymus Trachea 

Sternum 

Epididymis 

Submandib/Max 

Nose/Turb Sec 

Thoracic SC 

LN 

4 

Seminal vesicles 

Urinary bladder 

Femur 

Esophagus 

Sciatic Nerve 

Pancreas Lumbar SC 

Testes 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

SEX: Male 

PHASE: Dosing phase 

A

Tissue Gross Observations/Comments 

Colon ......... No gross observations on tissue. 

Heart . . No gross observations on tissue. 

Liver . . No gross observations on tissue. 

Mediastinal LN .... No gross observations on tissue. 

Submandib/Max LN ... No gross observations on tissue. 

Larynx: V-DVTC .... No gross observations on tissue. 

Larynx: V-SM-G . No gross observations on tissue. 

Nose/Turb Sec 2 .... No gross observations on tissue. 

Nose/Turb Sec 3 .... No gross observations on tissue. 

Parathyroid ...... No gross observations on tissue. 

Microscopic Observations/Comments 

LUMEN: NEMATODE(S), Present. 

MONONUCLEAR CELL INFILTRATE WITH MYOFIBER DEGENERATION/LOSS, Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

SINUSES: FREE ERYTHROCYTES, Minimal. SINUSES: FREE ERYTHROCYTES, Minimal. 

SUBMUCOSAL CYSTS WITH EOSINOPHILIC 

CRYSTALS, Present. 

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

NASOLACRIMAL DUCT(S): LUMEN-EOSINOPHILIC 

MATERIAL, UNILATERAL, Slight. 

NASOLACRIMAL DUCT(S): LUMEN-EOSINOPHILIC 

MATERIAL, UNILATERAL, Minimal. 

Tissue is unremarkable; one-of-pair 

missing. 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Rectum/Low Colon ... No gross observations on tissue. 

Thymus ........ No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

LUMEN: NEMATODE(S), Present. 

CONGESTION, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands 

Cecum 

Jejunum 

Nose/Turb Sec 1 

Thoracic SC 

Seminal vesicles 

Aorta 

Duodenum 

Kidneys 

Nose/Turb Sec 4 

Lumbar SC 

Testes 

Brain 

Epididymis 

Mesenteric LN 

Pancreas 

Salivary glands 

Thyroid ·gland 

Sternum Femur 

Esophagus Ileum 

Lungs Sciatic Nerve 

Prostate Cervical SC 

Spleen Stomach 

Trachea Urinary bladder 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Kidneys . · · · · · · · 

Cyst, Right, Clear, Fluid, 0.2 - 0.5 em 

Mediastinal LN . No gross observations on tissue. 

Larynx: V-DVTC .... No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Examined; 1 correlation found: 

CORTICAL CYST, Present. 

CORTEX: LYMPHOID CELL AGGREGATE(S), 

Minimal. 

CORTEX/CORTICO-MEDULLARY JUNCTION: 

TUBULE(S) DILATED, Minimal. 

- Cyst is on slide 28. 

SINUSES: FREE ERYTHROCYTES, Minimal. 

A

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

Larynx: V-SM-G .... No gross observations on tissue. 

Nose/Turb Sec 2 .... No gross observations on tissue. 

Prostate . No gross observations on tissue. 

Rectum/Low Colon ... No gross observations on tissue. 

SUBMUCOSAL CYSTS WITH EOSINOPHILIC 

CRYSTALS, Present. 

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

NASOLACRIMAL DUCT(S): LUMEN-EOSINOPHILIC 

MATERIAL, BILATERAL, Slight. 

LYMPHOID CELL AGGREGATE(S), Minimal. 

LUMEN: NEMATODE(S), Present. 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments Testes . No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

A

SEMINIFEROUS TUBULES: DILATED, UNILATERAL, 

Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum Colon Duodenum 

Heart Ileum Jejunum 

Submandib/Max LN Lungs Sciatic Nerve 

Nose/Turb Sec 4 Pancreas Parathyroid 

Lumbar SC Salivary glands Spleen 

Thyroid gland Thymus Trachea 

Sternum 

Epididymis Liver 

Nose/Turb Sec 1 

Cervical SC 

Stomach 

Urinary bladder 

Femur 

Esophagus 

Mesenteric LN 

Nose/Turb Sec 3 

Thoracic SC 

Seminal vesicles 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Epididymis 

Small, Right, Moderate 

Liver . . No gross observations on tissue. 

Larynx: V-SM-G .... No gross observations on tissue. 

Rectum/Low Colon ... No gross observations on tissue. 

Testes 

Small, Right, Severe 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable (with comment); 

one-of-pair missing. 

- Right epididymis is not presented for 

histopathology. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

Tissue is unremarkable (with comment). 

- Ventral seromucous glands are absent. 

LUMEN: NEMATODE(S), Present. 

Tissue is unremarkable (with comment); 

one-of-pair missing. 

- Right testis is not presented for 

histopathology. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain Sternum Femur 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Cecum Colon Duodenum Esophagus Heart 

Ileum Jejunum Kidneys Mesenteric LN Mediastinal LN 

Submandib/Max LN Lungs Larynx: V-DVTC Sciatic Nerve Nose/Turb Sec 1 

Nose/Turb Sec 2 Nose/Turb Sec 3 Nose/Turb Sec 4 Pancreas Prostate 

Parathyroid Cervical SC Thoracic SC Lumbar SC Salivary glands 

Spleen Stomach Seminal vesicles Thyroid gland Thymus 

Trachea Urinary bladder 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Kidneys . · · · · · · · 

Dilated Pelvis, Left, Moderate 

Liver . . No gross observations on tissue. Larynx: V-SM-G .... No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Examined; 1 correlation found: 

PELVIS: DILATED, UNILATERAL, Slight. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

A

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

- Intralumenal hemorrhage. 

Cervical SC ...... No gross observations on tissue. Tissue is unremarkable (with comment) . 

- Caudal medulla is present. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum Colon Duodenum 

Heart Ileum Jejunum 

Submandib/Max LN Lungs Larynx: V-DVTC 

Nose/Turb Sec 2 Nose/Turb Sec 3 Nose/Turb Sec 4 

Parathyroid Rectum/Low Colon Thoracic SC 

Sternum 

Epididymis 

Mesenteric LN 

Sciatic Nerve 

Pancreas 

Lumbar SC 

Spleen Stomach Seminal vesicles Testes 

Thymus Trachea Urinary bladder 

Femur 

Esophagus 

Mediastinal LN 

Nose/Turb Sec 1 

Prostate 

Salivary glands 

Thyroid gland 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

SEX: Male 

PHASE: Dosing phase 

A

Tissue Gross Observations/Comments 

Heart . . No gross observations on tissue. 

Liver . . No gross observations on tissue. 

Mediastinal LN .... No gross observations on tissue. 

Submandib/Max LN 

Discolored/ (post-fixation observation) 

Enlarged/ (post-fixation observation) 

Larynx: V-DVTC No gross observations on tissue. 

Prostate . No gross observations on tissue. 

Parathyroid ...... No gross observations on tissue. 

Microscopic Observations/Comments 

MONONUCLEAR CELL INFILTRATE WITH MYOFIBER 

DEGENERATION/LOSS, Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

SINUSES: FREE ERYTHROCYTES, Minimal. 

Examined; 1 correlation found: 

SINUSES: FREE ERYTHROCYTES, Slight. 

Examined; no correlation found 

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal

LYMPHOID CELL AGGREGATE(S), Minimal. 

Tissue is unremarkable; one-of-pair 

missing. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain Sternum Femur 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Tissues without comment under Gross Observations were within 

The following tissues were unremarkable microscopically: 

Cecum Colon Duodenum 

Ileum Jejunum Kidneys 

Larynx: V-SM-G Sciatic Nerve Nose/Turb Sec 1 

Nose/Turb Sec 4 Pancreas Rectum/Low Colon 

Lumbar SC Salivary glands Spleen 

Testes Thyroid gland Thymus 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

normal limits at necropsy. 

Epididymis 

Mesenteric LN 

Nose/Turb Sec 2 

Cervical SC 

Stomach 

Trachea 

Esophagus 

Lungs 

Nose/Turb Sec 3 

Thoracic SC 

Seminal vesicles 

Urinary bladder 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Kidneys ........ No gross observations on tissue. 

Liver . . No gross observations on tissue. 

Mediastinal LN .... No gross observations on tissue. 

Larynx: V-DVTC .... No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

BASOPHILIC TUBULES, Minimal. 

CORTEX: LYMPHOID CELL AGGREGATE(S), 

Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

SINUSES: FREE ERYTHROCYTES, Slight. 

A

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum Colon Duodenum 

Heart Ileum Jejunum Lungs Larynx: V-SM-G Sciatic Nerve 

Nose/Turb Sec 3 Nose/Turb Sec 4 Pancreas 

Rectum/Low Colon Cervical SC Thoracic SC 

Sternum 

Epididymis 

Mesenteric LN 

Nose/Turb Sec 1 

Prostate 

Lumbar SC 

Spleen Stomach Seminal vesicles Testes 

Thymus Trachea Urinary bladder 

Femur 

LN 

Esophagus 

Submandib/Max 

Nose/Turb Sec 2 

Parathyroid 

Salivary glands 

Thyroid gland 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Kidneys . · · · · · · · 

Dilated Pelvis/ (post-fixation observation) 

Liver . . No gross observations on tissue. 

Mediastinal LN 

Lungs ... 

Larynx: V-SM-G 

. No gross observations on tissue. 

Discolored, All lobes, Red, Foci, </= 0.1 

em, Moderate 

No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Examined; 1 correlation found: 

PELVIS: DILATED, Minimal. 

BASOPHILIC TUBULES, Slight. 

CORTEX: LYMPHOID CELL AGGREGATE(S), 

Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

Tissue is missing. 

Examined; 1 correlation found: 

HEMORRHAGE, Minimal. 

Tissue is unremarkable (with comment) . 

- Ventral seromucous glands are absent. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum Colon Duodenum 

Sternum 

Epididymis 

Femur 

Esophagus 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Heart Ileum Jejunum 

Larynx: V-DVTC Sciatic Nerve Nose/Turb Sec 1 

Nose/Turb Sec 4 Pancreas Prostate 

Cervical SC Thoracic SC Lumbar SC 

Stomach Seminal vesicles Testes 

Trachea Urinary bladder 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Mesenteric LN Submandib/Max LN 

Nose/Turb Sec 2 Nose/Turb Sec 3 

Parathyroid Rectum/Low Colon 

Salivary glands Spleen 

Thyroid gland Thymus 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Liver . . No gross observations on tissue. 

Larynx: V-DVTC .... No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

MONONUCLEAR CELL AGGREGATES, Minimal. 

A

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

Larynx: V-SM-G .... No gross observations on tissue. 

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum Colon Duodenum 

Heart Ileum Jejunum 

Mediastinal LN Submandib/Max LN Lungs 

Nose/Turb Sec 2 Nose/Turb Sec 3 Nose/Turb Sec 4 

Parathyroid Rectum/Low Colon Cervical SC 

Salivary glands Spleen Stomach 

Thyroid gland Thymus Trachea 

Sternum 

Epididymis 

Kidneys 

Sciatic Nerve 

Pancreas 

Thoracic SC 

Seminal vesicles 

Urinary bladder 

Femur 

Esophagus 

Mesenteric LN 

Nose/Turb Sec 1 

Prostate 

Lumbar SC 

Testes 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue 

Lungs ........ . 

Gross Observations/Comments 

Discolored, Left lobe(s), Red, Focus, 0.2-

0.5 em, Slight 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable. 

Examined; no correlation found 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue 

Lungs ........ . 

Gross Observations/Comments 

Discolored, All lobes, Red, Foci, 0.2 - 0.5 

em, Slight 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable. 

Examined; no correlation found 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Lungs . . No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable (with comment) . 

- Two lobes are atelectatic. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue 

Lungs .. · · · · · · · 

Gross Observations/Comments 

Discolored, All lobes, Red, Foci, 0.2 - 0.5 

em, Slight 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable (with comment) . 

Examined; no correlation found 

- One lobe is atelectatic. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Lungs ......... No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable (with comment) . 

- One lobe is atelectatic. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Lungs ......... No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable (with comment) . 

- One lobe is atelectatic. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Lungs ......... No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable (with comment) . 

- Four lobes are atelectatic. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue 

Lungs ........ . 

Gross Observations/Comments 

Discolored, Left lobe(s), Red, Focus, 0.2-

0.5 em, Slight 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable. 

Examined; no correlation found 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Epididymis 

Small, Bilateral, severe 

Lungs . . No gross observations on tissue. 

Seminal vesicles 

Small, Bilateral, Slight 

Testes 

Small, Bilateral, Moderate 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is missing. 

-Adipose tissue is present. 

Tissue is unremarkable (with comment). 

- One lobe is partially atelectatic. 

Examined; 1 correlation found: 

SECRETORY PRODUCT: DECREASED, Slight. 

Examined; 1 correlation found: 

GERMINAL EPITHELIUM: 

A

DEGENERATION/ATROPHY, BILATERAL, Severe. 

Soft, Bilateral, Slight Examined; 1 correlation found: 

GERMINAL EPITHELIUM: 

DEGENERATION/ATROPHY, BILATERAL, Severe

Thymus 

Discolored, Right lobe, Red, Slight Examined; 1 correlation found: 

CONGESTION, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue 

Lungs ........ . 

Gross Observations/Comments 

Discolored, All lobes, Red, Foci, </= 0.1 

em, Slight 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissue is unremarkable. 

Examined; no correlation found 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue 

Thymus 

Gross Observations/Comments 

Discolored, Right lobe, Red, Foci, </= 0.1 

em, Slight 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Examined; 1 correlation found: 

CONGESTION, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

SEX: Male 

PHASE: Dosing phase 

A

Tissue Gross Observations/Comments 

Sternum . . No gross observations on tissue. 

Kidneys ........ No gross observations on tissue. 

Liver . . No gross observations on tissue. 

Submandib/Max LN ... No gross observations on tissue. 

Lungs ........ . 

Larynx: V-DVTC 

Discolored, All lobes, Tan, Foci, 0.2 - 0.5 

em, Slight 

No gross observations on tissue. 

Nose/Turb Sec 1 .... No gross observations on tissue. 

Nose/Turb Sec 2 .... No gross observations on tissue. 

Microscopic Observations/Comments 

Tissue is unremarkable (with comment) . 

- Marrow is slightly hypocellular. 

CORTEX: PROXIMAL CONVOLUTED TUBULAR 

EPITHELIUM-EOSINOPHILIC/HYALINE DROPLETS, 

Slight. 

BASOPHILIC TUBULES, Minimal. 

CORTEX: LYMPHOID CELL AGGREGATE(S), 

Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

SINUSES: FREE ERYTHROCYTES, Slight. 

Tissue is unremarkable. 

Examined; no correlation found 

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

NASOLACRIMAL DUCT(S): LUMEN-HEMORRHAGE(S), 

UNILATERAL, Slight. 

NASOLACRIMAL DUCT(S): LUMEN-HEMORRHAGE(S), 

UNILATERAL, Slight. 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Rectum/Low Colon ... No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

LUMEN: NEMATODE(S), Present. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Colon Duodenum Epididymis 

Ileum Jejunum Mesenteric LN 

Sciatic Nerve Nose/Turb Sec 3 Nose/Turb Sec 4 

Parathyroid Cervical SC Thoracic SC 

Spleen Stomach Seminal vesicles 

Thymus Trachea Urinary bladder 

Femur 

Esophagus 

Mediastinal LN 

Pancreas 

Lumbar SC 

Testes 

Cecum 

Heart 

Larynx: V-SM-G 

Prostate 

Salivary glands 

Thyroid gland 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Kidneys . . No gross observations on tissue. 

Liver . . No gross observations on tissue. Larynx: V-DVTC .... No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

CORTEX: PROXIMAL CONVOLUTED TUBULAR 

EPITHELIUM-EOSINOPHILIC/HYALINE DROPLETS, 

Slight. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

A

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal

Nose/Turb Sec 1 .... No gross observations on tissue. 

ODONTOPATHY, UNILATERAL, Moderate. 

Nose/Turb Sec 2 .... No gross observations on tissue. 

ODONTOPATHY, BILATERAL, Slight. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum Colon Duodenum 

Heart 

Submandib/Max 

Nose/Turb Sec 

Cervical SC 

Stomach 

LN 

4 

Ileum 

Lungs 

Pancreas 

Thoracic SC 

Seminal vesicles 

Jejunum Larynx: V-SM-G 

Prostate 

Lumbar SC 

Testes 

Sternum 

Epididymis 

Mesenteric LN 

Sciatic Nerve 

Parathyroid 

Salivary glands 

Thyroid gland 

Femur 

Esophagus 

Mediastinal LN 

Nose/Turb Sec 3 

Rectum/Low Colon 

Spleen 

Thymus 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

SEX: Male 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments 

Trachea Urinary bladder 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Kidneys ........ No gross observations on tissue. 

Liver . . No gross observations on tissue. 

Mediastinal LN .... No gross observations on tissue. 

Larynx: V-SM-G .... No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

CORTEX: PROXIMAL CONVOLUTED TUBULAR 

EPITHELIUM-EOSINOPHILIC/HYALINE DROPLETS, 

Slight. 

BASOPHILIC TUBULES, Minimal. 

CORTEX: LYMPHOID CELL AGGREGATE(S), 

Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

SINUSES: FREE ERYTHROCYTES, Minimal. 

A

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum Colon Duodenum 

Heart Ileum Jejunum Lungs Larynx: V-DVTC Sciatic Nerve 

Nose/Turb Sec 3 Nose/Turb Sec 4 Pancreas 

Sternum 

Epididymis 

Mesenteric LN 

Nose/Turb Sec 1 

Prostate 

Femur 

LN 

Esophagus 

Submandib/Max 

Nose/Turb Sec 2 

Parathyroid 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Rectum/Low Colon 

Spleen 

Thymus 

Cervical SC 

Stomach 

Trachea 

Thoracic SC 

Seminal vesicles 

Urinary bladder 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Lumbar SC 

Testes 

Salivary glands 

Thyroid gland 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Heart . . No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

A

MONONUCLEAR CELL INFILTRATE WITH MYOFIBER 

DEGENERATION/LOSS, Minimal. 

Kidneys . . No gross observations on tissue. 

Liver . . No gross observations on tissue. 

Mediastinal LN .... No gross observations on tissue. 

Thymus ........ No gross observations on tissue. 

CORTEX: PROXIMAL CONVOLUTED TUBULAR 

EPITHELIUM-EOSINOPHILIC/HYALINE DROPLETS, 

Minimal. 

BASOPHILIC TUBULES, Minimal. MONONUCLEAR CELL AGGREGATES, Minimal. 

Tissue is unremarkable (with comment) . 

- Scant representation. 

CONGESTION, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum Colon Duodenum 

Ileum Jejunum Mesenteric LN 

Sternum 

Epididymis 

Submandib/Max LN 

Femur 

Esophagus 

Lungs 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Larynx : V-DVTC 

Nose/Turb Sec 3 

Rectum/Low Colon 

Spleen 

Trachea 

Larynx: V-SM-G 

Nose/Turb Sec 4 

Cervical SC 

Stomach 

Urinary bladder 

Sciatic Nerve 

Pancreas 

Thoracic SC 

Seminal vesicles 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Nose/Turb Sec 1 

Prostate 

Lumbar SC 

Testes 

Nose/Turb Sec 2 

Parathyroid 

Salivary glands 

Thyroid gland 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Kidneys ........ No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

CORTEX: PROXIMAL CONVOLUTED TUBULAR 

EPITHELIUM-EOSINOPHILIC/HYALINE DROPLETS, 

Slight. 

BASOPHILIC TUBULES, Minimal. 

CORTEX: LYMPHOID CELL AGGREGATE(S), 

Minimal. 

A

UROTHELIAL HYPERPLASIA, BILATERAL, Minima

Mediastinal LN . No gross observations on tissue. 

Larynx: V-SM-G .... No gross observations on tissue. 

Seminal vesicles 

Small, Right, Slight 

SINUSES: FREE ERYTHROCYTES, Minimal. 

Tissue is unremarkable (with comment) . 

- Ventral seromucous glands are absent. 

Examined; 1 correlation found: 

SECRETORY PRODUCT: DECREASED, 

UNILATERAL, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain Sternum Femur 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Cecum Colon Duodenum 

Heart 

Submandib/Max LN 

Nose/Turb Sec 2 

Parathyroid 

Salivary glands 

Thymus 

Ileum 

Lungs 

Nose/Turb Sec 3 

Rectum/Low Colon 

Spleen 

Trachea 

Jejunum Larynx: V-DVTC 

Nose/Turb Sec 4 

Cervical SC 

Stomach 

Urinary bladder 

Epididymis Liver 

Sciatic Nerve 

Pancreas 

Thoracic SC Testes 

Esophagus 

Mesenteric LN 

Nose/Turb Sec 1 

Prostate 

Lumbar SC 

Thyroid gland 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Kidneys ........ No gross observations on tissue. 

Liver . . No gross observations on tissue. 

Lungs . . No gross observations on tissue. 

Larynx: V-DVTC .... No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

CORTEX: PROXIMAL CONVOLUTED TUBULAR 

EPITHELIUM-EOSINOPHILIC/HYALINE DROPLETS, 

Slight. 

BASOPHILIC TUBULES, Slight. 

CORTEX: LYMPHOID CELL AGGREGATE(S), 

Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

Tissue is unremarkable (with comment) . 

- One lobe is atelectatic. 

A

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

Tissues without comment under Gross Observations were within 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum Colon Duodenum 

Heart Ileum 

Submandib/Max LN Larynx: V-SM-G 

Jejunum 

Sciatic Nerve 

normal limits at necropsy. 

Sternum 

Epididymis 

Mesenteric LN 

Nose/Turb Sec 1 

Femur 

Esophagus 

Mediastinal LN 

Nose/Turb Sec 2 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Nose/Turb Sec 3 

Rectum/Low Colon 

Spleen 

Thymus 

Nose/Turb Sec 4 

Cervical SC 

Stomach 

Trachea 

Pancreas 

Thoracic SC 

Seminal vesicles 

Urinary bladder 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Prostate 

Lumbar SC 

Testes 

Parathyroid 

Salivary glands 

Thyroid gland 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Kidneys ........ No gross observations on tissue. 

Mediastinal LN No gross observations on tissue. 

Testes No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

CORTEX: PROXIMAL CONVOLUTED TUBULAR 

EPITHELIUM-EOSINOPHILIC/HYALINE DROPLETS, 

Slight. 

CORTEX: LYMPHOID CELL AGGREGATE(S), 

Minimal. 

Tissue is missing. 

Tissue is unremarkable (with comment) . 

- Poor fixation. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum Colon Duodenum 

Heart 

Submandib/Max LN 

Nose/Turb Sec 1 

Prostate 

Lumbar SC 

Thyroid gland 

Ileum 

Lungs 

Nose/Turb Sec 2 

Parathyroid 

Salivary glands 

Thymus 

Jejunum Larynx: V-DVTC 

Nose/Turb Sec 3 

Rectum/Low Colon 

Spleen 

Trachea 

Sternum 

Epididymis 

Liver Larynx: V-SM-G 

Nose/Turb Sec 4 

Cervical SC 

Stomach 

Urinary bladder 

Femur 

Esophagus 

Mesenteric LN 

Sciatic Nerve 

Pancreas 

Thoracic SC 

Seminal vesicles 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Kidneys ........ No gross observations on tissue. 

Liver . . No gross observations on tissue. 

Lungs ........ . 

Discolored, All lobes, Red, Foci, 0.2 - 0.5 

em, Slight 

Larynx: V-DVTC .... No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

CORTEX: PROXIMAL CONVOLUTED TUBULAR 

EPITHELIUM-EOSINOPHILIC/HYALINE DROPLETS, 

Slight. 

CORTEX: LYMPHOID CELL AGGREGATE(S), 

Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

Examined; 1 correlation found: 

HEMORRHAGE, Minimal. 

- Two lobes are atelectatic. 

A

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal

Thymus ........ No gross observations on tissue. 

CONGESTION, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain Sternum Femur 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Cecum Colon Duodenum 

Heart 

Submandib/Max LN 

Nose/Turb Sec 3 

Rectum/Low Colon 

Spleen 

Trachea 

Ileum 

Larynx: V-SM-G 

Nose/Turb Sec 4 

Cervical SC 

Stomach 

Urinary bladder 

Jejunum 

Sciatic Nerve 

Pancreas 

Thoracic SC 

Seminal vesicles 

Epididymis 

Mesenteric LN 

Nose/Turb Sec 1 

Prostate 

Lumbar SC 

Testes 

Esophagus 

Mediastinal LN 

Nose/Turb Sec 2 

Parathyroid 

Salivary glands 

Thyroid gland 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Kidneys ........ No gross observations on tissue. 

Liver . . No gross observations on tissue. 

Lungs ........ . 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

CORTEX: PROXIMAL CONVOLUTED TUBULAR 

EPITHELIUM-EOSINOPHILIC/HYALINE DROPLETS, 

Slight. 

BASOPHILIC TUBULES, Minimal. 

CORTEX: LYMPHOID CELL AGGREGATE(S), 

Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

Discolored, All lobes, Red, Foci, </= 0.1 

em, Slight 

Tissue is unremarkable. 

Examined; no correlation found 

Testes No gross observations on tissue. Tissue is unremarkable (with comment) . 

- Poorly fixed. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain Sternum Femur 

Cecum Colon Duodenum Epididymis Esophagus 

Heart Ileum Jejunum Mesenteric LN Mediastinal LN 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Submandib/Max LN 

Nose/Turb Sec 2 

Parathyroid 

Salivary glands 

Thymus 

Larynx: V-DVTC 

Nose/Turb Sec 3 

Rectum/Low Colon 

Spleen 

Trachea 

Larynx: V-SM-G 

Nose/Turb Sec 4 

Cervical SC 

Stomach 

Urinary bladder 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Sciatic Nerve 

Pancreas 

Thoracic SC 

Seminal vesicles 

Nose/Turb Sec 1 

Prostate 

Lumbar SC 

Thyroid gland 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Kidneys . · · · · · · · 

Dilated Pelvis/ (post-fixation observation) 

Liver . . No gross observations on tissue. 

Mediastinal LN . No gross observations on tissue. 

Lungs ......... No gross observations on tissue. 

Larynx: V-DVTC .... No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Examined; 1 correlation found: 

PELVIS: DILATED, UNILATERAL, Minimal. 

CORTEX: PROXIMAL CONVOLUTED TUBULAR 

EPITHELIUM-EOSINOPHILIC/HYALINE DROPLETS, 

Slight. 

BASOPHILIC TUBULES, Slight. 

CORTEX: LYMPHOID CELL AGGREGATE(S), 

Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

SINUSES: FREE ERYTHROCYTES, Minimal. 

Tissue is unremarkable (with comment) . 

- Three lobes are atelectatic. 

A

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal

Larynx: V-SM-G .... No gross observations on tissue. 

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Slight. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain Sternum Femur 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Cecum Colon Duodenum 

Heart 

Sciatic Nerve 

Pancreas 

Thoracic SC 

Seminal vesicles 

Urinary bladder 

Ileum 

Nose/Turb Sec 1 

Prostate 

Lumbar SC 

Testes 

Jejunum 

Nose/Turb Sec 2 

Parathyroid 

Salivary glands 

Thyroid gland 

Epididymis 

Mesenteric LN 

Nose/Turb Sec 3 

Rectum/Low Colon 

Spleen 

Thymus 

LN 

Esophagus 

Submandib/Max 

Nose/Turb Sec 4 

Cervical SC 

Stomach 

Trachea 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue 

Lungs ....... · . 

Skin 

Gross Observations/Comments 

Discolored, All lobes, Tan, Foci, <I= 0.1 

em, Slight 

Hair Thin/Absent, foreleg, Slight/ Left 

Hair Thin/Absent, Hindleg, Slight/ Left 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Examined; 1 correlation found: 

VISCERAL PLEURA/CAPSULE: FIBROSIS, 

Focal, Minimal. 

Examined; 1 correlation found: 

HYPOTRICHOSIS, Minimal. 

Examined; 1 correlation found: 

HYPOTRICHOSIS, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue 

Vagina 

Gross Observations/Comments 

Nodule, Mucosa, Tan, Firm, 0.2 - 0.5 em/ 

Proximal 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Examined; 1 correlation found: 

CYST, Present. 

- Section comprises haired skin having 

deep dermal cyst lined by transitional 

epithelium. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

SEX: Female 

PHASE: Dosing phase 

A

Tissue Gross Observations/Comments 

Adrenal Glands .... No gross observations on tissue. 

Heart . . No gross observations on tissue. 

Kidneys ........ No gross observations on tissue. 

Liver . . No gross observations on tissue. 

Lungs ........ . 

Larynx: V-SM-G 

Discolored, All lobes, Red, Foci, </= 0.1 

em, Slight 

No gross observations on tissue. 

Nose/Turb Sec 2 .... No gross observations on tissue. 

Microscopic Observations/Comments 

CONGESTION, BILATERAL, Minimal. 

MONONUCLEAR CELL INFILTRATE WITH MYOFIBER 

DEGENERATION/LOSS, Minimal. 

CORTEX: LYMPHOID CELL AGGREGATE(S), 

Minimal. 

CORTEX/CORTICO-MEDULLARY JUNCTION: MINERAL 

DEPOSIT(S), Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

Tissue is unremarkable. 

Examined; no correlation found 

GRANULOMATOUS CHONDROPATHY, Slight. 

SEPTAL DEVIATION, Present. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Aorta Brain Sternum Femur Cecum 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments Tissues without comment under Gross Observations were within 

The following tissues were unremarkable microscopically: 

Colon Duodenum Esophagus 

Mesenteric LN Mediastinal LN Submandib/Max LN 

Nose/Turb Sec 1 Nose/Turb Sec 3 Nose/Turb Sec 4 

Parathyroid Rectum/Low Colon Cervical SC 

Salivary glands Spleen Stomach 

Trachea Urinary bladder Uterus 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

normal limits at necropsy. 

Ileum 

Larynx: V-DVTC 

Ovaries 

Thoracic SC 

Thyroid gland 

Jejunum 

Sciatic Nerve 

Pancreas 

Lumbar SC 

Thymus 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

SEX: Female 

PHASE: Dosing phase 

A

Tissue Gross Observations/Comments 

Heart . . No gross observations on tissue. 

Liver . . No gross observations on tissue. Larynx: V-SM-G . No gross observations on tissue. 

Nose/Turb Sec 2 .... No gross observations on tissue. 

Parathyroid ...... No gross observations on tissue. 

Uterus . No gross observations on tissue. 

Microscopic Observations/Comments 

MONONUCLEAR CELL INFILTRATE WITH MYOFIBER 

DEGENERATION/LOSS, Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

Tissue is unremarkable (with comment). 

- Ventral seromucous glands are absent. 

ODONTOPATHY, UNILATERAL, Minimal. 

- Periodontal hemorrhage, woven bone 

production, and disruption of ameloblasts 

and odontoblasts. 

Tissue is unremarkable; one-of-pair 

missing. 

LUMEN: DILATED, Slight. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain Sternum Femur 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Cecum Colon Duodenum 

Jejunum 

Lungs 

Nose/Turb Sec 4 

Thoracic SC Thyroid gland 

Kidneys 

Larynx: V-DVTC 

Ovaries 

Lumbar 

Thymus 

sc 

Mesenteric LN 

Sciatic Nerve 

Pancreas 

Salivary glands 

Trachea 

Esophagus 

Mediastinal LN 

Nose/Turb Sec 1 

Rectum/Low Colon 

Spleen 

Urinary bladder 

LN 

Ileum 

Submandib/Max 

Nose/Turb Sec 3 

Cervical SC 

Stomach 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

SEX: Female 

PHASE: Dosing phase 

A

Tissue Gross Observations/Comments 

Kidneys ........ No gross observations on tissue. 

Liver . . No gross observations on tissue. 

Mediastinal LN .... No gross observations on tissue. 

Larynx: V-DVTC .... No gross observations on tissue. Larynx: V-SM-G . No gross observations on tissue. 

Parathyroid ...... No gross observations on tissue. 

Rectum/Low Colon ... No gross observations on tissue. 

Microscopic Observations/Comments 

CORTEX: LYMPHOID CELL AGGREGATE(S), 

Minimal. 

CORTEX/CORTICO-MEDULLARY JUNCTION: MINERAL 

DEPOSIT(S), Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

SINUSES: FREE ERYTHROCYTES, Minimal. SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal

GRANULOMATOUS CHONDROPATHY, Minimal. 

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal

Tissue is unremarkable; one-of-pair 

missing. 

LUMEN: NEMATODE(S), Present. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain Sternum Femur 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Cecum Colon Duodenum 

Ileum Jejunum Mesenteric LN 

Sciatic Nerve Nose/Turb Sec 1 Nose/Turb Sec 2 

Ovaries Pancreas Cervical SC 

Salivary glands Spleen Stomach 

Trachea Urinary bladder Uterus 

Esophagus 

Submandib/Max LN 

Nose/Turb Sec 3 

Thoracic SC 

Thyroid gland 

Heart Lungs 

Nose/Turb Sec 4 

Lumbar SC 

Thymus 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Mediastinal LN .... No gross observations on tissue. 

Larynx: V-SM-G . No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

SINUSES: FREE ERYTHROCYTES, Minimal. 

A

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal

Thymus . No gross observations on tissue. 

MEDULLARY HYPERPLASIA, Minimal. 

Uterus . No gross observations on tissue. 

LUMEN: DILATED, Slight. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum 

Ileum 

Submandib/Max LN 

Nose/Turb Sec 2 

Parathyroid 

Salivary glands 

Urinary bladder 

Colon 

Jejunum 

Lungs 

Nose/Turb Sec 3 

Rectum/Low Colon 

Spleen 

Duodenum 

Kidneys 

Larynx: V-DVTC 

Nose/Turb Sec 4 

Cervical SC 

Stomach 

Sternum 

Esophagus 

Liver 

Sciatic Nerve 

Ovaries 

Thoracic SC Thyroid gland 

Femur 

Heart 

Mesenteric LN 

Nose/Turb Sec 1 

Pancreas 

Lumbar SC 

Trachea 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Adrenal Glands .... No gross observations on tissue. 

Liver . . No gross observations on tissue. 

Lungs ......... No gross observations on tissue. 

Parathyroid ...... No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable (with comment) . 

- One gland has scant representation of 

medulla. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

Tissue is unremarkable (with comment). 

- One lobe is atelectatic. 

Tissue is unremarkable; one-of-pair 

missing. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Aorta 

Colon 

Jejunum Larynx: V-DVTC 

Nose/Turb Sec 3 

Cervical SC 

Stomach 

Uterus 

Brain 

Duodenum 

Kidneys 

Larynx: V-SM-G 

Nose/Turb Sec 4 

Thoracic SC Thyroid gland 

Sternum 

Esophagus 

Mesenteric LN 

Sciatic Nerve 

Ovaries 

Lumbar SC 

Thymus 

Femur Cecum 

Heart Ileum Mediastinal LN Submandib/Max LN 

Nose/Turb Sec 1 Nose/Turb Sec 2 

Pancreas Rectum/Low Colon 

Salivary glands Spleen 

Trachea Urinary bladder 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

SEX: Female 

PHASE: Dosing phase 

A

Tissue Gross Observations/Comments 

Kidneys ........ No gross observations on tissue. 

Liver . . No gross observations on tissue. Larynx: V-SM-G .... No gross observations on tissue. 

Nose/Turb Sec 1 .... No gross observations on tissue. Nose/Turb Sec 2 .... No gross observations on tissue. 

Parathyroid ...... No gross observations on tissue. 

Skin 

Hair Thin/Absent, Thoracic, Moderate 

Microscopic Observations/Comments 

CORTEX/CORTICO-MEDULLARY JUNCTION: MINERAL 

DEPOSIT(S), Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

ODONTOPATHY, BILATERAL, Minimal. ODONTOPATHY, BILATERAL, Slight. 

Tissue is unremarkable; one-of-pair 

missing. 

Examined; 1 correlation found: 

HYPOTRICHOSIS, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain Sternum Femur 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Cecum Colon Duodenum 

Ileum Jejunum Mesenteric LN 

Lungs Larynx: V-DVTC Sciatic Nerve 

Ovaries Pancreas Rectum/Low Colon 

Lumbar SC Salivary glands Spleen 

Thymus Trachea Urinary bladder 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Esophagus Heart 

Mediastinal LN Submandib/Max LN 

Nose/Turb Sec 3 Nose/Turb Sec 4 

Cervical SC Thoracic SC 

Stomach Thyroid gland 

Uterus 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Colon ......... No gross observations on tissue. 

Liver . . No gross observations on tissue. Larynx: V-SM-G .... No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

LUMEN: NEMATODE(S), Present. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

GRANULOMATOUS CHONDROPATHY, Slight. 

A

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

Parathyroid ...... No gross observations on tissue. 

Skin 

Hair Thin/Absent, Hindpaws, Moderate 

Hair Thin/Absent, Forepaws, Severe 

Tissue is unremarkable; one-of-pair 

missing. 

Examined; 1 correlation found: 

HYPOTRICHOSIS, Minimal. 

Examined; 1 correlation found: 

HYPOTRICHOSIS, Minimal. 

EPITHELIUM: SQUAMOUS CELL HYPERPLASIA, Minimal. 

EPITHELIUM: HYPERKERATOSIS, Minimal. 

- Slide 28: skin from hindpaws; slide 29: 

skin from forepaws. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain Sternum Femur 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Cecum Duodenum Esophagus 

Jejunum Kidneys Mesenteric LN 

Lungs Larynx: V-DVTC Sciatic Nerve 

Nose/Turb Sec 3 Nose/Turb Sec 4 Ovaries 

Cervical SC Thoracic SC Lumbar SC 

Stomach Thyroid gland Thymus 

Uterus 

Heart 

Mediastinal LN 

Nose/Turb Sec 1 

Pancreas 

Salivary glands 

Trachea 

Ileum 

Submandib/Max LN 

Nose/Turb Sec 2 

Rectum/Low Colon 

Spleen 

Urinary bladder 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

SEX: Female 

PHASE: Dosing phase 

A

Tissue Gross Observations/Comments 

Kidneys ........ No gross observations on tissue. 

Larynx: V-DVTC .... No gross observations on tissue. Larynx: V-SM-G .... No gross observations on tissue. 

Parathyroid ...... No gross observations on tissue. 

Uterus . No gross observations on tissue. 

Microscopic Observations/Comments 

CORTEX/CORTICO-MEDULLARY JUNCTION: MINERAL 

DEPOSIT(S), Minimal. 

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, MinimalSUBMUCOSAL CYSTS WITH EOSINOPHILIC 

CRYSTALS, Present. 

Tissue is unremarkable (with comment) . 

- Ventral seromucous glands are absent. 

Tissue is unremarkable; one-of-pair 

missing. 

CERVIX: LYMPHANGIECTASIA, Present. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum 

Ileum 

Submandib/Max LN 

Colon 

Jejunum 

Lungs 

Duodenum 

Liver 

Sciatic Nerve 

Sternum 

Esophagus 

Mesenteric LN 

Nose/Turb Sec 1 

Femur 

Heart 

Mediastinal LN 

Nose/Turb Sec 2 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Nose/Turb Sec 3 

Cervical SC 

Stomach 

Nose/Turb Sec 4 

Thoracic SC Thyroid gland 

Ovaries 

Lumbar SC 

Thymus 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Pancreas 

Salivary glands 

Trachea 

Rectum/Low Colon 

Spleen 

Urinary bladder 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Kidneys ........ No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

A

CORTEX/CORTICO-MEDULLARY JUNCTION: MINERAL 

DEPOSIT(S), Minimal. 

Larynx: V-DVTC .... No gross observations on tissue. Larynx: V-SM-G .... No gross observations on tissue. 

SUBMUCOSAL CYSTS WITH EOSINOPHILIC 

CRYSTALS, Present. 

Tissue is unremarkable (with comment). 

- Ventral seromucous glands are absent. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum Colon Duodenum 

Ileum Jejunum Liver 

Submandib/Max LN Lungs Sciatic Nerve 

Nose/Turb Sec 3 Nose/Turb Sec 4 Ovaries 

Rectum/Low Colon Cervical SC Thoracic SC 

Spleen Stomach Thyroid gland 

Urinary bladder Uterus 

Sternum 

Esophagus 

Mesenteric LN 

Nose/Turb Sec 1 

Pancreas 

Lumbar SC 

Thymus 

Femur 

Heart 

Mediastinal LN 

Nose/Turb Sec 2 

Parathyroid 

Salivary glands 

Trachea 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Kidneys ........ No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

A

CORTEX/CORTICO-MEDULLARY JUNCTION: MINERAL 

DEPOSIT(S), Slight. 

Mediastinal LN .... No gross observations on tissue. 

Lungs . . No gross observations on tissue. 

Larynx: V-DVTC .... No gross observations on tissue. 

SINUSES: FREE ERYTHROCYTES, Minimal. 

Tissue is unremarkable (with comment) . 

- One lobe is atelectatic. 

SUBMUCOSAL CYSTS WITH EOSINOPHILIC 

CRYSTALS, Present. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum Colon Duodenum 

Ileum Jejunum Liver 

Larynx: V-SM-G Sciatic Nerve Nose/Turb Sec 1 

Nose/Turb Sec 4 Ovaries Pancreas 

Cervical SC Thoracic SC Lumbar SC 

Stomach Thyroid gland Thymus 

Uterus 

Sternum 

Esophagus 

Mesenteric LN 

Nose/Turb Sec 2 

Parathyroid 

Salivary glands 

Trachea 

Femur 

Heart 

Submandib/Max LN 

Nose/Turb Sec 3 

Rectum/Low Colon 

Spleen 

Urinary bladder 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

SEX: Female 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Lungs . . No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable (with comment). 

- Three lobes are atelectatic. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Lungs . . No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable (with comment) . 

- One lobe is atelectatic. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue 

Skin 

Gross Observations/Comments 

Hair Thin/Absent, Abdominal, Area, 6.0 -

6.9 em, Moderate 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Examined; 1 correlation found: 

HYPOTRICHOSIS, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

SEX: Female 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

SEX: Female 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Lungs . . No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable (with comment). 

- One lobe is atelectatic. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Lungs ........ . 

Discolored, All lobes, Pale, Slight 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable (with comment) . 

Examined; no correlation found 

- Three lobes are atelectatic. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

SEX: Female 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

SEX: Female 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

SEX: Female 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Skin 

Hair Thin/Absent, Forepaws, Severe 

Hair Thin/Absent, Hindpaws, Moderate 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Examined; 1 correlation found: 

HYPOTRICHOSIS, Minimal. 

Examined; 1 correlation found: 

HYPOTRICHOSIS, Minimal. 

EPITHELIUM: HYPERKERATOSIS, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

SEX: Female 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Lungs ......... No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable (with comment) . 

- One lobe is atelectatic. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

SEX: Female 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

SEX: Female 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Lungs ......... No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable (with comment) . 

- One lobe is atelectatic. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue 

Lungs ........ . 

Gross Observations/Comments 

Discolored, Left diaphragmatic lobe, Tan, 

Foci, </= 0.1 em, Slight 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable (with comment) . 

Examined; no correlation found 

- One lobe is atelectatic. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Lungs . . No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable (with comment). 

- One lobe is atelectatic. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue 

Lungs .... 

Gross Observations/Comments 

Discolored, All lobes, Red, Foci, 0.2 - 0.5 

em, Slight 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissue is unremarkable. 

Examined; no correlation found 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 32 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Recovery phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

No tissues examined. 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Larynx: V-DVTC . No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

A

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

Larynx: V-SM-G .... No gross observations on tissue. 

Parathyroid ...... No gross observations on tissue. 

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

Tissue is unremarkable; one-of-pair 

missing. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum Colon Duodenum 

Ileum Jejunum Kidneys 

Mediastinal LN Submandib/Max LN Lungs 

Nose/Turb Sec 2 Nose/Turb Sec 3 Nose/Turb Sec 4 

Rectum/Low Colon Cervical SC Thoracic SC 

Spleen Stomach Thyroid gland 

Urinary bladder Uterus 

Sternum 

Esophagus 

Liver 

Sciatic Nerve 

Ovaries 

Lumbar sc 

Thymus 

Femur 

Heart 

Mesenteric LN 

Nose/Turb Sec 1 

Pancreas 

Salivary glands 

Trachea 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Kidneys . . No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

BASOPHILIC TUBULES, Minimal. 

CORTEX: LYMPHOID CELL AGGREGATE(S), 

Minimal. 

A

CORTEX/CORTICO-MEDULLARY JUNCTION: MINERAL 

DEPOSIT(S), Minimal. 

Liver . . No gross observations on tissue. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

Larynx: V-SM-G .... No gross observations on tissue. Tissue is unremarkable (with comment) . 

- Ventral seromucous glands are absent. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum 

Ileum 

Lungs 

Nose/Turb Sec 3 

Rectum/Low Colon 

Spleen 

Urinary bladder 

Colon 

Jejunum 

Larynx: V-DVTC 

Nose/Turb Sec 4 

Cervical SC 

Stomach 

Uterus 

Duodenum 

Mesenteric LN 

Sciatic Nerve 

Ovaries 

Thoracic SC Thyroid gland 

Sternum 

Esophagus 

Mediastinal LN 

Nose/Turb Sec 1 

Pancreas 

Lumbar SC 

Thymus 

Femur 

Heart 

Submandib/Max LN 

Nose/Turb Sec 2 

Parathyroid 

Salivary glands 

Trachea 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Kidneys ........ No gross observations on tissue. 

Liver . . No gross observations on tissue. 

Lungs . . No gross observations on tissue. 

Larynx: V-DVTC .... No gross observations on tissue. Larynx: V-SM-G .... No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

BASOPHILIC TUBULES, Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

ALVEOLAR/INTRAALVEOLAR MACROPHAGES, 

Minimal. 

- Two lobes are atelectatic. 

SUBMUCOSAL CYSTS WITH EOSINOPHILIC 

CRYSTALS, Present. 

A

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

Nose/Turb Sec 1 .... No gross observations on tissue. Nose/Turb Sec 2 .... No gross observations on tissue. 

Ovaries . . No gross observations on tissue. 

Parathyroid ...... No gross observations on tissue. 

ODONTOPATHY, BILATERAL, Minimal. 

SEPTAL DEVIATION, Present. SEPTAL DEVIATION, Present. 

ODONTOPATHY, BILATERAL, Moderate. 

MINERALIZATION, Minimal. 

Tissue is unremarkable; one-of-pair 

missing. 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

SEX: Female 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments 

Rectum/Low Colon ... No gross observations on tissue. 

LUMEN: NEMATODE(S), Present. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain Sternum Femur 

Cecum Colon Duodenum Esophagus Heart 

Ileum Jejunum Mesenteric LN Mediastinal LN Submandib/Max LN 

Sciatic Nerve Nose/Turb Sec 3 Nose/Turb Sec 4 Pancreas Cervical SC 

Thoracic SC Lumbar SC Salivary glands Spleen Stomach Thyroid gland Thymus Trachea Urinary bladder Uterus 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

Liver . . No gross observations on tissue. 

Mediastinal LN . No gross observations on tissue. 

Larynx: V-SM-G . No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

MONONUCLEAR CELL AGGREGATES, Minimal. 

SINUSES: FREE ERYTHROCYTES, Minimal. 

A

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum 

Ileum 

Lungs 

Nose/Turb Sec 3 

Rectum/Low Colon 

Spleen 

Urinary bladder 

Colon 

Jejunum 

Larynx: V-DVTC 

Nose/Turb Sec 4 

Cervical SC 

Stomach 

Uterus 

Duodenum 

Kidneys 

Sciatic Nerve 

Ovaries 

Thoracic SC 

Thyroid gland 

Sternum 

Esophagus 

Mesenteric LN 

Nose/Turb Sec 1 

Pancreas 

Lumbar SC 

Thymus 

Femur 

Heart 

Submandib/Max LN 

Nose/Turb Sec 2 

Parathyroid 

Salivary glands 

Trachea 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

SEX: Female 

PHASE: Dosing phase 

A

Tissue Gross Observations/Comments 

Kidneys ........ No gross observations on tissue. 

Liver . . No gross observations on tissue. Larynx: V-DVTC .... No gross observations on tissue. Larynx: V-SM-G .... No gross observations on tissue. 

Nose/Turb Sec 1 .... No gross observations on tissue. 

Nose/Turb Sec 2 .... No gross observations on tissue. 

Parathyroid ...... No gross observations on tissue. 

Stomach . . No gross observations on tissue. 

Microscopic Observations/Comments 

CORTEX/CORTICO-MEDULLARY JUNCTION: MINERAL 

DEPOSIT(S), Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

GRANULOMATOUS CHONDROPATHY, Minimal. 

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

NASOLACRIMAL DUCT(S): LUMEN-HEMORRHAGE(S), 

UNILATERAL, Minimal. 

NASOLACRIMAL DUCT(S): LUMEN-HEMORRHAGE(S), 

UNILATERAL, Minimal. 

Tissue is unremarkable; one-of-pair 

missing. 

GLANDULAR ECTASIA, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain Sternum Femur 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 90 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Cecum Colon Duodenum 

Ileum Jejunum Mesenteric LN 

Lungs Sciatic Nerve Nose/Turb Sec 3 

Pancreas Rectum/Low Colon Cervical SC 

Salivary glands Spleen Thyroid gland 

Urinary bladder Uterus 

Esophagus 

Mediastinal LN 

Nose/Turb Sec 4 

Thoracic SC 

Thymus 

Heart 

Submandib/Max LN 

Ovaries 

Lumbar SC 

Trachea 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Liver . . No gross observations on tissue. 

Larynx: V-DVTC .... No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

MONONUCLEAR CELL AGGREGATES, Minimal. 

A

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

Larynx: V-SM-G . No gross observations on tissue. Tissue is unremarkable (with comment). 

- Ventral seromucous glands are absent. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum 

Ileum 

Submandib/Max LN 

Nose/Turb Sec 3 

Rectum/Low Colon 

Spleen 

Urinary bladder 

Colon 

Jejunum 

Lungs 

Nose/Turb Sec 4 

Cervical SC 

Stomach 

Uterus 

Duodenum 

Kidneys 

Sciatic Nerve 

Ovaries 

Thoracic SC Thyroid gland 

Sternum 

Esophagus 

Mesenteric LN 

Nose/Turb Sec 1 

Pancreas 

Lumbar SC 

Thymus 

Femur 

Heart 

Mediastinal LN 

Nose/Turb Sec 2 

Parathyroid 

Salivary glands 

Trachea 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Liver . . No gross observations on tissue. 

Mediastinal LN . No gross observations on tissue. 

Larynx: V-SM-G .... No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

MONONUCLEAR CELL AGGREGATES, Minimal. 

SINUSES: FREE ERYTHROCYTES, Minimal. 

A

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum Colon Duodenum 

Ileum Jejunum Kidneys 

Lungs Larynx: V-DVTC Sciatic Nerve 

Nose/Turb Sec 3 Nose/Turb Sec 4 Ovaries 

Rectum/Low Colon Cervical SC Thoracic SC 

Spleen Stomach Thyroid gland 

Urinary bladder Uterus 

Sternum 

Esophagus 

Mesenteric LN 

Nose/Turb Sec 1 

Pancreas 

Lumbar SC 

Thymus 

Femur 

Heart 

Submandib/Max 

Nose/Turb Sec 

LN 

2 

Parathyroid 

Salivary glands 

Trachea 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

SEX: Female 

PHASE: Dosing phase 

A

Tissue Gross Observations/Comments 

Kidneys . . No gross observations on tissue. 

Liver . . No gross observations on tissue. 

Mediastinal LN . No gross observations on tissue. 

Lungs ........ . 

Discolored, All lobes, Tan, Foci, </= 0.1 

em, Slight 

Larynx: V-DVTC .... No gross observations on tissue. 

Larynx: V-SM-G .... No gross observations on tissue. 

Parathyroid ...... No gross observations on tissue. Rectum/Low Colon ... No gross observations on tissue. 

Microscopic Observations/Comments 

CORTEX/CORTICO-MEDULLARY JUNCTION: MINERAL 

DEPOSIT(S), Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

SINUSES: FREE ERYTHROCYTES, Slight. 

Examined; 1 correlation found: 

ALVEOLAR/INTRAALVEOLAR MACROPHAGES, 

Minimal. 

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

GRANULOMATOUS CHONDROPATHY, Minimal. 

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

Tissue is unremarkable; one-of-pair 

missing. 

LUMEN: NEMATODE(S), Present. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain Sternum Femur 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments Tissues without comment under Gross Observations were within 

The following tissues were unremarkable microscopically: 

Cecum Colon Duodenum 

Ileum Jejunum Mesenteric LN 

Nose/Turb Sec 1 Nose/Turb Sec 2 Nose/Turb Sec 3 

Pancreas Cervical SC Thoracic SC 

Spleen Stomach Thyroid gland 

Urinary bladder Uterus 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

normal limits at necropsy. 

Esophagus 

Submandib/Max LN 

Nose/Turb Sec 4 

Lumbar SC 

Thymus 

Heart 

Sciatic Nerve 

Ovaries 

Salivary glands 

Trachea 

A
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

SEX: Female 

PHASE: Dosing phase 

A

Tissue Gross Observations/Comments 

Kidneys ........ No gross observations on tissue. 

Liver . . No gross observations on tissue. Larynx: V-SM-G . No gross observations on tissue. 

Parathyroid ...... No gross observations on tissue. 

Microscopic Observations/Comments 

CORTEX/CORTICO-MEDULLARY JUNCTION: MINERAL 

DEPOSIT(S), Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

Tissue is unremarkable; one-of-pair 

missing. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum Colon Duodenum 

Ileum Jejunum Mesenteric LN 

Lungs Larynx: V-DVTC Sciatic Nerve 

Nose/Turb Sec 3 Nose/Turb Sec 4 Ovaries 

Cervical SC Thoracic sc Lumbar SC 

Stomach Thyroid gland Thymus 

Uterus 

Sternum 

Esophagus 

Mediastinal LN 

Nose/Turb Sec 1 

Pancreas 

Salivary glands 

Trachea 

Femur 

Heart 

Submandib/Max LN 

Nose/Turb Sec 2 

Rectum/Low Colon 

Spleen 

Urinary bladder 
15-Dec-11; 17:46 

Huntingdon Life Sciences, Inc. 006130 

Main Study 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 91 

Tissue Gross Observations/Comments 

Kidneys . . No gross observations on tissue. 

Liver . . No gross observations on tissue. Larynx: V-SM-G .... No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

CORTEX: LYMPHOID CELL AGGREGATE(S), 

Minimal. 

MONONUCLEAR CELL AGGREGATES, Minimal. 

GRANULOMATOUS CHONDROPATHY, Minimal. 

A

SUBMUCOSAL LYMPHOCYTIC AGGREGATE, Minimal. 

Uterus . No gross observations on tissue. 

LUMEN: DILATED, Slight. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Adrenal Glands Aorta Brain 

Cecum Colon Duodenum 

Ileum Jejunum Mesenteric LN 

Lungs Larynx: V-DVTC Sciatic Nerve 

Nose/Turb Sec 3 Nose/Turb Sec 4 Ovaries 

Rectum/Low Colon Cervical SC Thoracic SC 

Spleen Stomach Thyroid gland 

Urinary bladder 

Sternum 

Esophagus 

Mediastinal LN 

Nose/Turb Sec 1 

Pancreas 

Lumbar SC 

Thymus 

Femur 

LN 

Heart 

Submandib/Max 

Nose/Turb Sec 2 

Parathyroid 

Salivary glands 

Trachea 
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

Tissue Gross Observations/Comments 

Kidneys . · · · · · · · 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Discolored, Bilateral, Tan, Foci, 0.2 - 0.5 

em, Moderate 

Examined; 1 correlation found: 

DILATED TUBULES, BILATERAL, Slight. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Brain Eyes Lungs 

Sural Nerve Trigeminal Gang Dorsal Root Gang 

Optic nerve Cervical SC Thoracic SC 

Sciatic Nerve 

Dorsal Root Fibr Lumbar SC 

Tibial Nerve 

Ventrl Root Fibr 

Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Brain Eyes Lungs 

Sural Nerve Trigeminal Gang Dorsal Root Gang 

Optic nerve Cervical SC Thoracic SC 

Sciatic Nerve 

Dorsal Root Fibr Lumbar SC 

Tibial Nerve 

Ventrl Root Fibr 

Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

SEX: Male 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Brain Eyes Lungs Sciatic Nerve Tibial Nerve 

Sural Nerve Trigeminal Gang Dorsal Root Gang Dorsal Root Fibr Ventrl Root Fibr 

Optic nerve Cervical SC Thoracic SC Lumbar SC Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

SEX: Male 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments 

Lungs ........ . Tissue is unremarkable. 

Discolored, All lobes/ (post-fixation Examined; no correlation found 

observation) 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Brain Eyes Sciatic Nerve Tibial Nerve Sural Nerve 

Trigeminal Gang Dorsal Root Gang Dorsal Root Fibr Ventrl Root Fibr Optic nerve 

Cervical SC Thoracic SC Lumbar SC Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

Tissue Gross Observations/Comments Tissues without comment under Gross Observations were within 

The following tissues were unremarkable microscopically: 

Brain Eyes Lungs 

Sural Nerve Trigeminal Gang Dorsal Root Gang 

Optic nerve Cervical SC Thoracic SC 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

normal limits at necropsy. 

Sciatic Nerve 

Dorsal Root Fibr 

Lumbar SC 

Tibial Nerve 

Ventrl Root Fibr 

Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs Trachea 

A
15-Dec-ll; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

Tissue Gross Observations/Comments 

Lungs . . No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

HEMORRHAGE(S), Multifocal, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

Tissue Gross Observations/Comments 

Lungs . . No gross observations on tissue. 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

ALVEOLAR MACROPHAGES, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

Tissue Gross Observations/Comments 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Brain 

Sural Nerve 

Optic nerve 

Eyes 

Trigeminal Gang 

Cervical SC 

Lungs 

Dorsal Root Gang 

Thoracic SC 

Sciatic Nerve 

Dorsal Root Fibr Lumbar SC 

Tibial Nerve 

Ventrl Root Fibr 

Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

SEX: Male 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments 

Lungs . . No gross observations on tissue. 

HEMORRHAGE(S), Focal, Minimal. 

Thymus 

Discolored, Red, Foci, </= 0.1 em, Slight Examined; 1 correlation found: 

CONGESTION, Slight. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Brain Eyes Sciatic Nerve Tibial Nerve Sural Nerve 

Trigeminal Gang Dorsal Root Gang Dorsal Root Fibr Ventrl Root Fibr Optic nerve 

Cervical SC Thoracic SC Lumbar SC Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

SEX: Male 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Brain Eyes Lungs Sciatic Nerve Tibial Nerve 

Sural Nerve Trigeminal Gang Dorsal Root Gang Dorsal Root Fibr Ventrl Root Fibr 

Optic nerve Cervical SC Thoracic SC Lumbar SC Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

Tissue 

Kidneys . . · · · · · · 

Skin 

Gross Observations/Comments 

Discolored, Bilateral, Tan, Foci, </= 0.1 

em, Moderate 

Hair Thin/Absent, Slight/ Left lateral 

Scab, Cervical, Tan, 1.0 - 1.5 em/ With 

hair loss 

SEX: Male 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Examined; no correlation found 

PROXIMAL TUBULAR EPITHELIUM: EOSINOPHILIC 

CRYSTALS, Slight. 

Examined; 1 correlation found: 

HYPOTRICHOSIS, Slight. 

Examined; 1 correlation found: 

EPITHELIUM: ULCER(S), Marked. 

SURFACE BACTERIA, Present. 

DERMAL GRANULATION TISSUE, Moderate. 

NEUTROPHILIC INFILTRATE, Moderate. 

- Slide "0" has cervical skin. Slide "P" 

has left lateral skin. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Brain Eyes Lungs Sciatic Nerve Tibial Nerve 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

SEX: Male 

PHASE: Dosing phase 

Tissue 

Sural Nerve 

Optic nerve 

Gross Observations/Comments Microscopic Observations/Comments 

Trigeminal Gang 

Cervical SC 

Dorsal Root Gang Dorsal Root Fibr Ventrl Root Fibr 

Thoracic SC Lumbar SC Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

SEX: Male 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments 

Lungs ......... No gross observations on tissue. 

HEMORRHAGE(S), Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Brain Eyes Sciatic Nerve Tibial Nerve Sural Nerve 

Trigeminal Gang Dorsal Root Gang Dorsal Root Fibr Ventrl Root Fibr Optic nerve 

Cervical SC Thoracic SC Lumbar SC Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Brain 

Sural Nerve 

Optic nerve 

Eyes 

Trigeminal Gang 

Cervical SC 

Lungs 

Dorsal Root Gang 

Thoracic SC 

Sciatic Nerve 

Dorsal Root Fibr 

Lumbar SC 

Tibial Nerve 

Ventrl Root Fibr 

Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

Tissue Gross Observations/Comments 

Eyes ......... No gross observations on tissue. 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

A

CILIARY BODY: MONONUCLEAR CELL INFILTRATE, 

Minimal. 

Sural Nerve . . No gross observations on tissue. Tissue is missing. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Brain Lungs Sciatic Nerve Tibial Nerve 

Dorsal Root Gang Dorsal Root Fibr Ventr1 Root Fibr Optic nerve 

Thoracic SC Lumbar SC Trachea 

Trigeminal Gang 

Cervical SC 
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Brain Eyes Lungs 

Sural Nerve Trigeminal Gang Dorsal Root Gang 

Optic nerve Cervical SC Thoracic SC 

Sciatic Nerve 

Dorsal Root Fibr Lumbar SC 

Tibial Nerve 

Ventrl Root Fibr 

Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

SEX: Female 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments 

Sural Nerve . . No gross observations on tissue. Tissue is unremarkable (with comment) . 

- The longitudinal section is missing. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Brain Eyes Lungs Sciatic Nerve Tibial Nerve 

Trigeminal Gang Dorsal Root Gang Dorsal Root Fibr Ventrl Root Fibr Optic nerve 

Cervical SC Thoracic SC Lumbar SC Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

SEX: Female 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Brain Eyes Lungs Sciatic Nerve Tibial Nerve 

Sural Nerve Trigeminal Gang Dorsal Root Gang Dorsal Root Fibr Ventrl Root Fibr 

Optic nerve Cervical SC Thoracic SC Lumbar SC Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

SEX: Female 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

SEX: Female 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

SEX: Female 

PHASE: Dosing phase 

Tissue 

Lungs . . . . . . . . . 

Gross Observations/Comments 

Discolored, All lobes/ (post-fixation 

observation) 

Microscopic Observations/Comments 

Tissue is unremarkable. 

Examined; no correlation found 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

SEX: Female 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

SEX: Female 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

Tissue 

Lungs .. · · · · · · · 

Gross Observations/Comments 

Discolored, All lobes/ (post-fixation 

observation) 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissue is unremarkable. 

Examined; no correlation found 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Trachea 

AN

G
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs Trachea 

A
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

Tissue 

Lungs ........ . 

Gross Observations/Comments 

Discolored, Right diaphragmatic lobe, Tan, 

Foci, <I= 0.1 em, Slight 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Examined; no correlation found 

HEMORRHAGE(S), Focal, Minimal. 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Trachea 

AN

G
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Lungs Trachea 

AN

G
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

Tissue Gross Observations/Comments 

Tissues without comment under Gross Observations were within 

The following tissues were unremarkable microscopically: 

Brain Eyes Lungs 

Sural Nerve Trigeminal Gang Dorsal Root Gang 

Optic nerve Cervical SC Thoracic SC 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

normal limits at necropsy. 

Sciatic Nerve 

Dorsal Root Fibr 

Lumbar SC 

Tibial Nerve 

Ventrl Root Fibr 

Trachea 

AN

G
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Brain Eyes Lungs 

Sural Nerve Trigeminal Gang Dorsal Root Gang 

Optic nerve Cervical SC Thoracic SC 

Sciatic Nerve 

Dorsal Root Fibr 

Lumbar SC 

Tibial Nerve 

Ventrl Root Fibr 

Trachea 

AN

G
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 93 

SEX: Female 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Brain Eyes Lungs Sciatic Nerve Tibial Nerve 

Sural Nerve Trigeminal Gang Dorsal Root Gang Dorsal Root Fibr Ventrl Root Fibr 

Optic nerve Cervical SC Thoracic SC Lumbar SC Trachea 

AN

G
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

Tissue Gross Observations/Comments 

SEX: Female 

PHASE: Dosing phase 

Microscopic Observations/Comments 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Brain 

Sural Nerve 

Optic nerve 

Eyes 

Trigeminal Gang 

Cervical SC 

Lungs 

Dorsal Root Gang 

Thoracic SC 

Sciatic Nerve 

Dorsal Root Fibr 

Lumbar SC 

Tibial Nerve 

Ventrl Root Fibr 

Trachea 

AN

G
15-Dec-11; 19:15 

Huntingdon Life Sciences, Inc. 006130N 

Neurotox 

Gasoline DIPE Vapor Condensate: A 13-Week Whole-Body Inhalation 

Toxicity Study in Rats with Neurotoxicity Assessments and 4-Week 

In Vivo Genotoxicity and Immunotoxicity Assessments 

Individual Animal Gross and Microscopic Observations 

STATUS: Final phase sacrifice 

PHASE DAY OF DEATH: 94 

SEX: Female 

PHASE: Dosing phase 

Tissue Gross Observations/Comments Microscopic Observations/Comments 

Lungs ........ . Tissue is unremarkable. 

Discolored, Right diaphragmatic lobe, Tan, Examined; no correlation found 

Focus, 0.2 - 0.5 em, Slight 

Tissues without comment under Gross Observations were within normal limits at necropsy. 

The following tissues were unremarkable microscopically: 

Brain Eyes Sciatic Nerve Tibial Nerve Sural Nerve 

Trigeminal Gang Dorsal Root Gang Dorsal Root Fibr Ventrl Root Fibr Optic nerve 

Cervical SC Thoracic SC Lumbar SC Trachea 

A