Methyl Anthranilate

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OFFICE OF

CHEMICAL SAFETY AND 

POLLUTION PREVENTION

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

WASHINGTON D.C., 20460

MEMORANDUM DATE: 08/24/2011

SUBJECT: Non-target Organism and Endangered Species Screening Risk Assessment 

for the Methyl Anthranilate (Benzoic Acid, 2-Amino, Methyl Ester) 

Registration Review Preliminary Work Plan

Chemical Class: Biochemical

PC Code: 128725

CAS Number: 134-20-3

Tolerance Exemptions: 40 CFR 180.

FROM: Russell S. Jones, Ph.D., Senior Biologist

Biochemical Pesticides Branch

Biopesticides & Pollution Prevention Division

TO: Chris Pfeifer, Regulatory Action Leader

Biochemical Pesticides Branch

Biopesticides & Pollution Prevention Division

EXECUTIVE SUMMARY

The bird repellent, Methyl Anthranilate, in a naturally occurring flavoring substance in 

grapes. The repellent action arises from the stimulation of the trigemimal nerve in the head 

of a bird resulting in a pain response (Kirifides et. al., 2004). 

Based on the available data and information, the Agency does not foresee the need for new 

data or for new risk assessments for this active ingredient. Non-target organism hazard and 

exposure information as well as Agency risk assessments on Methyl Anthranilate were 

evaluated against current safety standards established by the Agency’s scientific policies and 

regulations and it was determined that there is no need to conduct additional risk 

assessments. The active ingredient is a naturally occurring substance, has a non-toxic mode 

of action, and is of low toxicity. There is reasonable certainty of no harm to non-target 

organisms when Methyl Anthranilate is applied in accordance with EPA-approved product 
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labeling. A screening level assessment was conducted to evaluate the existing non-target 

organism data base. 

Toxic endpoints have not been identified for non-target mammals and non-target birds on an 

acute and dietary basis in laboratory testing (respective non-definitive endpoints of >5000 

mg/kg and >500 mg/kg/day). The active ingredient is moderately toxic to slightly toxic to 

freshwater fish on a acute basis (9.12 ppm to 42.56 ppm), and practically non-toxic on a 

dietary basis (>1000 mg/kg in diet) in laboratory testing. It is slightly toxic to aquatic 

invertebrates (17.0 to 29.1 ppm). Toxic endpoints have not been established for non-target 

insects (honey bees). Non-target terrestrial plants can tolerate >40,000 ppm of Methyl 

Anthranilate before exhibiting any observable foliar desiccation or necrosis (MRID 

42740204). Based on the highest respective application rates for terrestrial and aquatic use 

sites, calculated Risk Quotients (RQs) are all <0.05 for all taxa. 

Based on the fact that Methyl Anthranilate is relatively non-toxic to non-target organisms 

and on its use pattern and use instructions, EPA has determined that Methyl Anthranilate will 

have "No Effect" on any currently listed threatened or endangered species or any designated 

critical habitat. 

1. Description of the Active Ingredient

Common Name: Methyl Anthranilate

Chemical Name: Benzoic Acid, 2-Amino, Methyl Ester

Synonyms: Anthranilic Acid, methyl ester

Methyl-2-amonobenzoate

Methyl o-aminobenzoate

o-Carbomethoxyaniline

Neroli Oil

Nevoli Oil

CAS Number: 134-20-3

Empirical Formula: C8H9NO2

Figure 1. Structure of Methyl Anthranilate
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Methyl Anthranilate is a repellent used to repel pest birds from food use and non-food use 

sites. The active ingredient is employed in pesticide products used on landfills, tailing ponds, 

commercial/industrial water impoundments, temporary pools, decorative fountains, non-fish 

bearing waters, electrical substations, farmsteads, agricultural sites, structures, buildings, 

airports & other open areas; ornamental trees & shrubs; turf; lakes, ponds, harbors, boat 

docks, aquatic production sites (non-fish bearing waters such as hatcheries and culture 

ponds), pome fruits, stone fruits, cereal grains, blueberries, sunflowers, table grapes, and turf.

Methyl Anthranilate was classified as a Biochemical Pesticide Active Ingredient by the OPP 

Biotechnology Work Group August 24, 1992 (EPA, 1992).

2. Registered End-Use Products and Maximum Use Rates

There are 7 currently registered End-Use Products (EPs) containing Methyl Anthranilate as 

their active ingredient (see Appendix Table 1). 

3. Ecological Hazards

Adequate non-target toxicology data/information are available to support registration of

Methyl Anthranilate. All non-target organism toxicology data requirements for Methyl

Anthranilate have been satisfied. No additional data are required. 

There are no concerns for any non-target organisms when Methyl Anthranilate is applied in 

accordance with EPA-approved label use directions. Toxic endpoints have not been 

identified for non-target mammals and non-target birds on an acute and dietary basis in 

laboratory testing. The active ingredient is moderately toxic to slightly toxic to freshwater 

fish on a acute basis, and practically non-toxic on a dietary basis in laboratory testing. It is 

slightly toxic to aquatic invertebrates. Toxic endpoints have not been established for nontarget insects (honey bees). Non-target terrestrial plants can tolerate >40,000 ppm of Methyl 

Anthranilate before exhibiting any observable foliar desiccation or necrosis (MRID 

42740204). 

A review of the ECOTOX database did not reveal any additional non-target toxicity data or 

endpoints that were lower than those previously evaluated by the Agency. 

4. Environmental Fate and Ground Water Data

The need for environmental fate and groundwater data was not triggered because results of 

the acute toxicity assessment did not trigger any additional Tier I studies. Some data, 

however, are available from studies submitted in support of a Tolerance Exemption and from 

external technical sources. Residues of Methyl Anthranilate (MA) applied to food crops are 

expected to dissipate to background levels before they are distributed for consumption. 
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Methyl anthranilate is extremely volatile (vapor pressure of 2.7 x 10-2 mm Hg at 25oC) and 

will rapidly dissipate into the surrounding atmosphere from foliar surfaces to which it has 

been applied, and is expected to have an atmospheric half-life of 11 hours (HSDB, 2004). 

Based on its vapor pressure and low water solubility, MA is also expected to volatilize 

rapidly from water surfaces (HSDB, 2004) in as little as 16 days. 

Data obtained from the technical literature suggest a range of degradation rates depending 

upon environmental conditions. Aronov and Clark (1996) report that MA is not subject to 

hydrolysis under laboratory conditions between pH 5 to pH 9 in aqueous phosphate buffers, 

but is susceptible to degradation by UV light (up to 42% after 27 days of illuminance at 1.25 

mW cm-2, equivalent to natural sunlight at noon at 40onorth latitude). Microbial activity was 

observed to be a significant degradation pathway with 22% loss after nine days and 100% 

loss after 20 days at optimal conditions of light and temperature (a 12:12 hr light:dark cycle 

and 23oC). Laboratory studies (Askham, 1992) with MA on glass slides demonstrated that 

MA was extremely photolabile with 50% loss after two days of exposure to full sunlight and 

was undetectable after four days exposure.

Avery et. al. (1995) reported residues on rice as 11.7 ppm immediately following an 

application of ReJex-It AG-36 (14.5% a.i.) at 6.2 lb a.i./A, which rapidly declined over the 

next 14 days with a half-life of 7 days (interpolated by reviewer based on study author data). 

The application rate used in this study is equivalent to the maximum approved label 

application rate for food crops (see Appendix Table 1). 

Based on data from empirical studies, MA residues will be low immediately following 

application and will rapidly dissipate in terrestrial environments under the combined 

influences of UV light and microbial degradation (Aronov & Clark, 1996; Askham, 1992) . 

Low solubility in water coupled with rapid volatilization and microbial degradation will 

result in minimal residues in aquatic habitats. 

5. Ecological Exposure and Risk Characterization

Methyl Anthranilate is a naturally-occurring substance in plants such as corn, sunflowers, 

grapes (33 ppm) and cherries (35 ppm), as well as cocoa and black tea (EPA, 1994). Honey

made from citrus floral sources has been reported to contain from 3.60 to 5.04 mg/kg of MA 

(Ferreres et. a., 1994; White & Bryant, 1996, unpublished study). It is extremely volatile 

and degrades rapidly into non-toxic components such as anthranilic acid. Numerous studies 

are available evaluating residues on crops and aquatic environments following application of 

Methyl Anthranilate at maximum label use rates for the respective use sites. 

Terrestrial Residues: Data from magnitude of the residue studies on apples, corn (sweet), 

and sunflower, submitted in support of an Experimental Use Permit in 2000 (66550-EUP001; EPA, 2000) confirm the rapid dissipation of MA. Residues on whole apples treated 

with a single foliar application of MA at 2.29 lbs a.i./A declined from 1.79 ppm at 1-hour 

posttreatment to <0.055 ppm (non-detectable) by 14 days posttreatment (MRID 45065105). 

Residues on sweet corn treated with a single foliar application of MA at 0.573 lbs a.i./A were 
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1.65 ppm at 1-hour posttreatment. Following a second treatment after a five-day application 

interval, residues were 0.374 ppm 10-days after the second application (MRID 45065103). 

Residues on sunflowers treated with a single foliar application of MA at 0.573 lbs a.i./A were 

0.011 ppm at 1-hour posttreatment. Following a second treatment after a five-day

application interval, residues were 0.233 ppm 10-days after the second application (MRID 

45065104). 

Avery et. al. (1995) reported residues on rice as 11.7 ppm immediately following an 

application of ReJex-It AG-36 (14.5% a.i.) at 6.2 lb a.i./A, which rapidly declined over the 

next 14 days with a half-life of 7 days (interpolated by reviewer based on study author data). 

The application rate used in this study is equivalent to the maximum approved label 

application rate for food crops (see Appendix Table 1). 

The residue value reported by Avery et. al. (1995) was used to calculate Risk Quotients 

(RQs) for mammals and birds following terrestrial applications of methyl Anthranilate at the 

maximum label used rate. 

Aquatic Residues: Magnitude of the residue studies have been conducted for both direct 

application and fogging applications of Methyl Anthranilate products to aquatic sites. 

In a catfish toxicity study (Dorr et. al. 1998), a 40% liquid MA end-use product (ReJex-iT 

AG 40; EPA Reg. No. 58035-7; not currently registered) was applied directly to the surface 

of an aquaculture pond at an exaggerated rate of 80 lb a.i./A water surface (10X the 

maximum label rate for currently registered products). The maximum concentration of MA 

was 4.44 ppm at a depth of 24 inches following seven applications over a two week period. 

If mathematically adjusted to a 1X rate, the calculated residues are estimated to be 

approximately 0.444 ppm. 

A magnitude of the residue study (MRIDs 45210201, 45210202, & 45229701) was 

conducted to determine the maximum amount of MA residues that would occur in a pond 

following fogging applications using two different types of foggers (thermal and ultra low 

volume) and a product that is no longer registered for use (ReJex-iT TP-40; EPA Reg. No. 

58035-7; 40% a.i.). The samples were not replicated which precluded statistical analysis and 

an assessment of sample variability, and there were five anomalously high MA residue 

measurements (5.97 ppm to 60.47 ppm) that were well outside the range of the majority of 

samples (total = 26), which were in the range of <0.016 ppm to 1.6 ppm. In addition, the 

unusually high values were well outside the values reported by Dorr et. al. (1998), following 

direct spray application to pond. Therefore, these data are considered unreliable for purposes 

of non-target organism risk assessment. It was observed, however, that MA content of water 

samples generally decreased with distance from the foggers. 

Fog application rates are an order of magnitude below that of direct spray applications (0.21 

lb a.i/A vs. 8.0 lb a.i./A). The Tier I Rice Model equation (EPA, 2007) was used to estimate 

environmental concentrations in water following a fog application at maximum label use 

rates (0.21 lbs a.i./A or 0.24 kg a.i./ HA) and assuming a Koc of 250 for MA (HSDB, 2004):
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Cw = mai/(0.00105 + (0.00013 * Kd))

Where: Cw = water concentration [μg/L]

mai' = mass applied per unit area [kg/ha]

Kd = water-sediment partitioning coefficient [L/kg] (and Kd = 0.01Koc)

Koc = organic carbon partitioning coefficient [L/kg]

Then: Cw = 0.24/(0.00105 + (0.00013 * 2.5)) = 0.175 ppm

This value is less that the calculated EEC of 0.444 ppm (estimated using data from Dorr et. 

al., 1998). The conservative EEC of 0.444 ppm will be used to evaluate risk to non-target 

aquatic organisms when Methyl Anthranilate is applied at the maximum label use rates. 

6. Threatened and Endangered Species Assessment

A qualitative risk assessment can be conducted with acute and dietary mammal and avian 

toxicity data; aquatic organism LC50 and EC50 data; and terrestrial plant and insect (honey 

bee) data from obtained from guideline studies, non-guideline studies, and the open technical 

literature (Appendix Table 2), in conjunction with the label use information for Methyl

Anthranilate from the product labels (Appendix Table 1). Although none of the labels 

specified any limitation on the number of applications, there is sufficient information to 

conduct a qualitative endangered species risk assessment due to the minimal toxicity of 

Methyl Anthranilate to non-target species and its rapid biodegradation in the environment. 

Toxic endpoints were not found for mammals, birds, and terrestrial insects in laboratory

testing (Appendix Table 2). Field testing on cherries, blueberries, grapes, and sweet corn 

demonstrated that >40000 ppm of methyl anthranilate was required to induce slight necrosis 

and desiccation of foliage (MRID 42740204). The product is considered slightly toxic for 

five of six fish species tested (LD50 range: 16.23 to 42.56 ppm) and moderately toxic (LD50

= 9.12 ppm) to one fish species (Appendix Table 2). 

Mammals: Mammalian (rat) acute oral toxicity is >5000 mg/kg (Toxicity Category IV; 

Appendix Table 2). The subchronic (90-day) dietary No Observable Effects Level (NOEL) 

was >500 ppm (Appendix Table 2). The non-definitive endpoints are respectively 

approximately 430X and 43X greater than the observed maximum environmental 

concentrations of 11.7 ppm for MA residues (Avery et. al., 1995) on terrestrial foliage. 

Based on the data and the Guidance for Using Non-Definitive Endpoints in Evaluating Risks 

to Listed and Non-listed Animal Species (EPA, 2010), there are no concerns for non-target 

mammals. 

Birds: Avian acute oral toxicity >2250 mg/kg (Practically Non-toxic; Appendix Table 2). 

avian dietary toxicity was >5620 ppm (Practically Non-toxic; Appendix Table 2). The nondefinitive endpoints are respectively approximately 192X and 480X greater than the 

observed maximum environmental concentrations of 11.7 ppm for MA residues (Avery et. 
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al., 1995) on terrestrial foliage. Based on the data and the Guidance for Using NonDefinitive Endpoints in Evaluating Risks to Listed and Non-listed Animal Species (EPA, 

2010), there are no concerns for non-target birds. 

Fish: Freshwater fish 96-hr LC50s ranged from 9.12 ppm to 42.56 ppm across five fish 

species, which are categorized as moderately toxic to slightly toxic (Appendix Table 2). 

Using the most conservative endpoint for fish in laboratory testing (Bluegill sunfish; 9.12

ppm) and the measured calculated maximum EEC of MA in water (0.44 ppm), the Risk 

Quotient (RQ) was calculated as:

RQ = 0.44 ppm = 0.019

 9.12 ppm

The RQ is well below any Level of Concern (LOC) for non-target fish, including threatened 

and endangered species. 

Aquatic Invertebrates: Aquatic Invertebrate EC50s for daphnids ranged from 17.0 to 29.1 

ppm which is categorized as moderately to slightly toxic (Appendix Table 2). Using the 

most conservative endpoint for aquatic invertebrates (17.0 ppm) in laboratory testing and the 

maximum estimated environmental concentration (EEC) of MA in water following a direct 

spray application to water (0.44 ppm), the RQ was calculated as:

RQ = 0.44 ppm = 0.048 

17.0 ppm

The RQ is below any LOC for non-target aquatic invertebrates, including threatened and 

endangered species. 

Plants: Methyl anthranilate is a naturally-occurring substance found in many plant species

such as corn, sunflowers, grapes (33 ppm) and cherries (35 ppm). It is extremely volatile and 

degrades rapidly into non-toxic components such as anthranilic acid. MA has no known 

toxic effects when applied exogenously to plants at the proposed rates of application. 

Reports of leaf desiccation in some studies were later attributed to the presence of certain 

surfactants in the end-use products and were not caused by MA (Askham, 1992; MRID 

42740204). In a field study using leaves of blueberries, cherries, and grapes, it was 

demonstrated that non-target terrestrial plants can tolerate >40,000 ppm of Methyl 

Anthranilate before exhibiting any observable, but minor foliar desiccation or necrosis 

(MRID 42740204). Minor foliar desiccation or necrosis was observed at 80000 ppm, but no 

plant mortality was reported (Askham, 1992; MRID 42740204). . 

Using the most conservative non-definitive endpoint for plants (40000 ppm) and the 

maximum observable residues on plants following a foliar spray application at the maximum 

label use rate (11.7 ppm; Avery et. al., 1995), the RQ was calculated as:
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RQ = 11.7 ppm = 0.002 

40000 ppm

The RQ is well below any LOC for non-target plants, including threatened and endangered 

species. 

Insects: Non-target insect contact toxicity LD50 for insects (honey bee) was >25 ug/bee (see 

Appendix Table 2), which is categorized as practically non-toxic. No toxic effects were 

observed at the highest rate used in laboratory testing. Methyl anthranilate is a naturallyoccurring substance found in many plant species such as corn, sunflowers, grapes (33 ppm) 

and cherries (35 ppm) to which pollinators and other insects are exposed on a regular basis

(EPA, 1994). Endogneous levels of MA reported for grapes and cherries are higher than 

residues measured on foliage (11.7 ppm) following a single application of MA at maximum 

label use rates (Avery et. al., 1995). Honey made from citrus floral sources has been reported 

to contain from 3.60 to 5.04 mg/kg of MA (Ferreres et. a., 1994; White & Bryant, 1996, 

unpublished study) indicating regualr dietary and contact exposure to naturally-occurring 

MA by pollinators. Based on these data, the Agency has no concerns to non-target insects. 

CONCLUSIONS: There are no concerns for any non-target organism when Methyl 

Anthranilate is applied in accordance with EPA-approved product labeling. 

Based on the fact that Methyl Anthranilate has minimal toxicity to non-target organisms and 

on its use pattern and use instructions, EPA has determined that Methyl Anthranilate will 

have "No Effect" on any currently listed threatened or endangered species or any designated 

critical habitat. 

REFERENCES

Aronov, E. V. and L. Clark. 1996. Degradation studies of the non-lethal bird repellent, 

methyl anthranilate. Pesticide Science 47: 355-362. 

Askham, L. R. and J. K. Fellman. 1989. Use of DMA to reduce robin depredation on 

cherries. Proceedings of the Great Plains Wildlands Damage Control Workshop 9: 116-119.

Askham, L. R. 1992. Efficacy of methyl anthranilate as a bird repellent on cherries, 

blueberries and grapes. Proceedings of the Fifteenth Vertebrate Pest Conference. J. E. 

Borrecco & R. E. Marsh, Eds. Published at University of California, Davis. 1992, pp. 137-

141. 

Avery, M. L. 1989. Experimental evaluation of partial repellent treatment for reducing bird 

damage to crops. Journal of Applied Ecology 26: 433-439.
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Avery, M. L., D. G. Decker, J. S. Humphrey, S. Aronov, S. D. Lindscombe, and M. O. Way. 

1995. Methyl anthranilate as a rice seed treatment to deter birds. Journal of Wildlife 

management 59(1): 50-56. 

Dorr, B., L. Clark, J. E. Glahn, I. Mezine. 1998. Evaluation of a methyl anthranilate-based 

bird repellent. Toxicity to channel catfish Ictalurus punctatus and effect on Great Blue 

Heron Ardea herodias feeding behavior. Journal of the World Aquaculture Society 29(4): 

451-462.

EPA. 1992. Classification of Methyl Anthranilate (Chem. No. 128725) as a Conventional 

Chemical or as a Biochemical Pesticide. Memorandum from J. T. McClintock to D. B. 

Peacock. 

EPA. 1994. SAB review of the product chemistry, toxicology, summary information, and 

toxicology and residue chemistry waivers submitted for the registration of technical methyl 

anthranilate and an end-use product, “Bird Shield Repellent.” Product chemistry (Case# 

008125; Chemical # 128725; Submission # S455617; DP barcode D197915). Memorandum 

from S. K. Reilly to R. Forrest, dated 11/17/1994. 

EPA. 2000. Experimental use permit for Bird Shield Repellent (EPA Reg. No. 6550-EUP001) containing 26.4% methyl anthranilate as its active ingredient in or on corn sunflowers, 

and apples (chemical no. 128725): Evaluation of residue chemistry and analytical method. 

MRIDs 450651-01, -02, -03, -04, and -05; Case no. 066100; Submission no. S580610; DP 

barcode D266613. Memorandum from F. Toghrol to J. Downing, dated 08/16/2000. 

EPA. 2007. Guidance for Tier I Estimation of Aqueous Pesticide Concentrations in Rice 

Paddies. Memorandum from S. Bradbury to EFED/OPP, dated 05/08/2007. 

EPA. 2010. Guidance for Using Non-Definitive Endpoints in Evaluating Risks to Listed and 

Non-listed Animal Species. Memorandum from D. J. Brady (Director, Environmental Fate 

and Effects Division) to Environmental Fate and Effects Division Scientists, dated 

08/03/2010. 

Ferreres, F., J. M. Giner, and F. A. Tomas-Barberan. 1994. A comparative study of 

hesperetin and methyl anthranilate as markers of floral origin of citrus honey. Journal of the 

Science of Food and Agricultural Science 65(3): 371-372. 

Harpaz, S. and L. Clark. 2006. Effects of addition of a bird repellent to fish diets on their 

growth and bioaccumulation. Aquaculture Research 37: 132-138.

Hazardous Substances Data Base (HSDB). 2004. National Library of Medicine/TOXNET 

(05/25/2004 update). 

http://toxnet.nlm.nih.gov/cgi-bin/sis/search/a?dbs+hsdb:@term+@DOCNO+1008
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Kirifides, M. L., M. P. Kurnellas, L. Clark, and B. P. Bryant. Calcium responses of chicken 

trigeminal nerve ganglion neurons to methyl anthranilate and capsaicin. Journal of 

Experimental Biology 207: 715-722. 

White, J. W. and V. M. Bryant 1996. Assessing citrus honey quality: Pollen and methyl 

anthranilate content. (Unpublished study). http://www.honey.com/white/pdf/white199-

assessing-citrus-honey-quality.pdf

cc: C. Pfeifer, R.S. Jones, BPPD Subject File/IHAD

R. S. Jones, Ph.D., Sr. Scientist, FT, OPY: 08/24/2011
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APPENDIX

Appendix Table 1. Currently registered products and maximum label use rates for 

each product label. Highest use rates are boldfaced. 

Product EPA 

Reg. 

No.

Methyl 

Anthranilate

Percentage

Maximum Use Rate per Acre

per Application 

Application

Intervals

Avian ControlTM 33162-1 20.00% Ground: 8 lb a.i./A surface water 11 - 4 days

Fog: 0.21 lbs a.i./A 21 - 4 days

ReJex-It Fog ForceTM 58037-7 40.00% Ground: 8 lb a.i./A surface water 11 - 4 days

Outdoor Fog: 0.21 lbs a.i./A 2, 3 1 - 4 days

ReJex-It AG-36TM 58035-9 14.50% Ground: 6.18 lb a.i./A 4 Not specified

Aerial: 6.18 lb a.i./A 5 Not specified

Aerial: 6.18 lb a.i./A

6

22 days 

Ground: 0.07 lb a.i./A 74 days

ReJex-It Fog Force AR-20TM 58035-15 20.00% Outdoor Fog: 0.21 lb a.i./A 2, 7 1 - 4 days

Bird Shield Repellent 66550-1 20.00% Ground/aerial: 0.28 – 5.73 lb a.i./A 8 Not specified

Aerial: 0.28 lb a.i./A 9 Not specified

Ground: 2.29 lb a.i./A 10 Not specified

Ground: 4.58 lb a.i./A 7

7 – 14 days

Ground: 0.07 lb a.i./gal Spot spray

Ground: 0.12 lbs a.i./gal 1 Added to pool

Liquid Fence Goose Repellent 72041-2 20.72% Ground: 0.5 gal/40K sq ft 7 Not specified

Avex 83359-4 26.40% Ground/aerial: 5.73 lb a.i./A 8 Not specified

Aerial: 0.28 lb a.i./A 9 Not specified

Ground: 2.29 lb a.i./A 10 Not specified

Ground: 2.29 – 4.58 lb a.i./A 77 – 14 days

Ground: 0.07 lb a.i./gal Spot spray

Ground: 0.12 lbs a.i./gal 1 Added to pool

1 Ground application to landfills, tailing ponds, commercial/industrial water impoundments, temporary pools, 

decorative fountains, non-fish bearing waters.

2 Electrical substations, farmsteads, agricultural sites, structures, buildings, airports & other open areas; 

ornamental trees & shrubs; turf; lakes, ponds, harbors, boat docks, aquatic production sites (non-fish bearing 

waters such as hatcheries and culture ponds)

3 Tree fruits & cereal grains

4 Apples, stonefruits, blueberries, & grapes

5 Cereal Grains

6 Sunflowers

7 Turf

8 Pome fruit, stone fruit

9 Sunflower, blueberries

10 Table grapes
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Appendix Table 2. Summary of Non-Target Organism Data

Study Type/OCSPP Guideline LD50/LC50/EC50

Results

Toxicity 

Category

MRID

Acute Oral Toxicity

/OCSPP 870.1100 

>5000 mg/kg (rat) Tox Category IV 42608702

90-day Feeding (rat)

/OCSPP 870.

>500 mg/kg/day No effects on growth 

or survival 42151904

Avian Acute Oral Toxicity

/OCSPP 850.2100 

>2036 ppm

(Bobwhite quail) Practically non-toxic 42966902

>2250 ppm

(Bobwhite quail) Practically non-toxic 43610701

Avian Dietary Toxicity

/OCSPP 850.

>5620 ppm

(Mallard duck)

Practically non-toxic 42608808

>2200

(White crowned sparrow)

Practically non-toxic 42966903

>5259 ppm

(Mallard duck)

Practically non-toxic 44741501

Freshwater Fish Acute Toxicity, 96 hr

/OCSPP 850.1075 

9.12 ppm 1, 2

(Bluegill sunfish)

Moderately toxic 42718202

42.56 ppm 

NOEC = 33.6 ppm

(bluegill)

Slightly toxic 43610702

22.91 ppm 1

(Rainbow trout)

Slightly toxic 42966901

25.40 ppm 2

(Rainbow trout)

Slightly toxic 43610703

16.23 ppm 1

(Channel catfish)

Slightly toxic 42699803

32.25 ppm 1

(Atlantic salmon)

Slightly toxic 42995101

Freshwater Fish Dietary Toxicity, 12-hr

/Non-Guideline

>1000 mg/kg

(striped bass & African 

cichlid)

No effects on growth 

or survival

Harpaz & 

Clark, 2006

Aquatic Freshwater Invertebrate Toxicity, 48-hr

/OCSPP 850.1010 

17.0 mg/L Slightly toxic 41895207

29.1 mg/L Slightly toxic 42718203

Non-target Plants >40000 ppm on 

blueberries, cherries, 

grapes, and raspberries3

Practically non-toxic 42740204

Non-target Insects (Honey Bee Contact Toxicity, >25 ng/bee Practically non-toxic 41623704
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Study Type/OCSPP Guideline LD50/LC50/EC50

Results

Toxicity 

Category

MRID

48-hr)/OCSPP 850.3020

1

Static conditions

2

Flow-through conditions

3 Based on visual observations of foliar necrosis/desiccation
Methyl Anthranilate

PC Code: 128725

Non-Target Organism Hazard Assessment and Endangered Species Screening Risk Assessment for Registration 

Review Preliminary Work Plan

14

Appendix Table 3. Maximum N-6-BA residues following a single application, or two 

consecutive applications at the maximum label use rates. 1

Environmental Matrix

Residues (ppm)

following single 

application at 0.039lb 

a.i./A 2

Residues (ppm)

following two 

applications at 0.041 lb 

a.i./A with a 3-day 

interval 3

Residues (ppm)

following two 

applications at 0.165 

lb a.i./A with a 7-day 

interval 3

Short Grass

Tall Grass

Broadleaf Plants/Small Insects

Fruits/Pods/Seedlings/Large 

insects

1 Dietary-based Estimated Environmental Concentration (EEC) calculated using T-REX model (EPA, 2005). 

2

Promalin Plant Growth Regulator (EPA Reg. No. 73049-1)

3 Riteway (EPA Reg. No. 71368-60)

Appendix Table 4. Maximum N-6-BA residues in aquatic environments following a 

single application, or two consecutive airblast applications at the maximum label use 

rates (airblast/orchard application method). 1

Environmental Matrix

Residues (ppb)

following single 

application at 0.039lb 

a.i./A 2

Residues (ppb)

following two 

applications at 0.041 lb 

a.i./A with a 3-day 

interval 3

Residues (ppb)

following two 

applications at 0.165 

lb a.i./A with a 7-day 

interval 3

Peak GEEC

Maximum 4-Day Avg. GEEC

Maximum 21-Day Avg. GEEC

Maximum 60-Day Avg. GEEC

Maximum 90-Day Avg. GEEC

1 Estimated Environmental Concentration (EEC) calculated using GENEEC2 model (EPA, 2001). 

2

Promalin Plant Growth Regulator (EPA Reg. No. 73049-1)

3 Riteway (EPA Reg. No. 71368-60)