U.S. Department of the Interior

U.S. Geological Survey

Open-File Report 2009–1053

Prepared in cooperation with the Missouri Department of Conservation

Determination of Polychlorinated Biphenyls, Selected 

Persistent Organochlorine Pesticides, and Polybrominated 

Flame Retardants in Fillets of Fishes from the 2007 Missouri 

Department of Conservation Monitoring Program

Determination of Polychlorinated Biphenyls, 

Selected Persistent Organochlorine Pesticides, 

and Polybrominated Flame Retardants in Fillets 

of Fishes from the 2007 Missouri Department of 

Conservation Monitoring Program

By Robert W. Gale, Carl E. Orazio, and Michael J. McKee

Prepared in cooperation with the Missouri Department of Conservation

Open-File Report 2009–1053

U.S. Department of the Interior

U.S. Geological Survey
U.S. Department of the Interior

KEN SALAZAR, Secretary

U.S. Geological Survey

Suzette M. Kimball, Acting Director

U.S. Geological Survey, Reston, Virginia: 2009

For more information on the USGS—the Federal source for science about the Earth, its natural and living resources, 

natural hazards, and the environment, visit http://www.usgs.gov or call 1-888-ASK-USGS

For an overview of USGS information products, including maps, imagery, and publications, 

visit http://www.usgs.gov/pubprod

To order this and other USGS information products, visit http://store.usgs.gov

Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the 

U.S. Government.

Although this report is in the public domain, permission must be secured from the individual copyright owners to 

reproduce any copyrighted materials contained within this report.

Suggested citation:

Gale, R.W., Orazio, C.E., and McKee, M.J., 2009, Determination of polychlorinated biphenyls, selected persistent 

organochlorine pesticides, and polybrominated flame retardants in fillets of fishes from the 2007 Missouri Department 

of Conservation Monitoring Programs: U.S. Geological Survey Open-File Report 2009–1053, 20 p.
iii

Contents

Abstract...........................................................................................................................................................1

Introduction.....................................................................................................................................................1

Sample History ...............................................................................................................................................1

Methods...........................................................................................................................................................2

Compositing and Homogenization .....................................................................................................2

Preparation ............................................................................................................................................2

Quality Control................................................................................................................................................3

Results and Discussion.................................................................................................................................3

Lipids.......................................................................................................................................................3

Total PCBs ..............................................................................................................................................4

Organochlorine Pesticides..................................................................................................................4

Selected PBDEs ....................................................................................................................................4

References Cited............................................................................................................................................4

Tables 1–4........................................................................................................................................................7

Tables

1. Sample description and percent lipid values in Missouri Department of 

Conservation 2007 fish fillets ......................................................................................................8

2. Total polychlorinated biphenyl and estimated Aroclor® concentrations and 

Aroclor® percentages in Missouri Department of Conservation 2007 fish fillets.............. 9

3. Organochlorine pesticide concentrations in Missouri Department of 

Conservation 2007 fish fillets ....................................................................................................11

4. Polybrominated diphenyl ethers concentrations and percentages in Missouri 

Department of Conservation 2007 fish fillets..........................................................................19
iv

Conversion Factors and Datums

SI to Inch/Pound

Multiply By To obtain

Volume

milliliter (mL) 0.03382 ounce, fluid (fl. oz)

Mass

gram (g) 0.03527 ounce, avoirdupois (oz)

nanogram (ng) 1 x 10-9 grams

nanogram (ng) 3.527 x 10-11 ounce, avoirdupois (oz)

Concentration

nanogram per gram (ng/g) = part per billion (ppb; 109)

nanogram per milliliter (ng/mL) = part per billion (ppb; 109)

Temperature in degrees Celsius (°C) may be converted to degrees Fahrenheit (°F) as follows:

 °F=(1.8×°C)+32

Concentrations of chemical constituents in solid materials (fish fillets) are given in nanogram 

per gram (ng/g). Concentrations of chemical constituents in liquid solutions (calibration 

standards) are given in nanogram per milliliter (ng/mL).
Determination of Polychlorinated Biphenyls, 

Selected Persistent Organochlorine Pesticides, and 

Polybrominated Flame Retardants in Fillets of Fishes 

from the 2007 Missouri Department of Conservation 

Monitoring Programs

By Robert W. Gale1, Carl E. Orazio1, and Michael J. McKee2

1

U.S. Geological Survey.

2

Missouri Department of Conservation.

Abstract

This report presents the results of a study to determine 

polychlorinated biphenyl, organochlorine pesticide, and polybrominated diphenylether flame retardant concentrations in 

selected fishes from lakes and streams across Missouri. Fillets 

were collected from each fish sample and after homogenization, compositing, and preparation, analyte concentrations 

were determined with dual column capillary gas chromatography-electron-capture detection. Total concentrations of 

polychlorinated biphenyls in samples ranged from background 

levels of about 50 to 300 nanograms per gram. In samples 

with elevated contaminant concentrations, chlordanes, DDTrelated chemicals, and dieldrin constituted the primary classes 

of pesticides present, and ranged from 5 to 75 nanograms per 

gram. Total concentrations of polybrominated diphenyl ethers 

in samples ranged from background levels of 5 to 86 nanograms per gram. Channel catfish from the upper and lower 

Blue River and lake sturgeon from the Mississippi River at 

Saverton exhibited different polybrominated diphenyl ethers 

ratios. Concentrations of polychlorinated biphenyls, chlordanes, DDT-related compounds, and polybrominated diphenyl 

ethers all were greatest in samples of channel catfish from the 

upper and lower Blue River, and in samples of lake sturgeon 

from the Mississippi River at Saverton.

Introduction

The Missouri Department of Conservation (MDC) 

began a long-term state-wide fish monitoring program in 

1984 (Bataille, 2003; May and others, 2007; Gale and others, 

2008). The program is designed to characterize contaminant 

concentrations at 20 to 30 lakes and streams throughout Missouri by annually collecting predator and bottom-feeding 

species samples. The sites that are monitored each year 

vary based on data needs, budgets, and personnel resources. 

Emphasis is on human health and, therefore, incorporates 

fish fillets, composite samples, and sample replication at each 

site to assess any potential fish consumption risks. In 2007, 

26 samples from 7 sites were selected for sampling as part 

of the MDC General Contaminant Monitoring Program. The 

following predator and bottom-feeding species were selected 

based on the need for organic contaminant information and the 

potentially greater risk for human consumption: largemouth 

bass (Micropterus salmoides), channel catfish (Ictalurus 

punctatus), lake sturgeon (Acipenser fulvescens), and common 

carp (Cyprinus carpio). The MDC has requested the collaborative assistance of the U.S. Geological Survey (USGS) for this 

monitoring program because of its experience with aquatic 

biota monitoring projects and expertise in the preparation and 

analysis of fish for trace-level organic contaminants. 

Sample History

A shipment of 26 fish fillet composites was received by 

the USGS on November 19, 2007. Upon receipt, the shipment 

was assigned USGS batch number 1428 and sample identification numbers 41267–41292. All samples were logged into the 

data base and assigned data-base numbers. The identification 

numbers, MDC field identification, location descriptions, and 

sample percent lipids for each sample are listed in table 1, at 

the back of this report.

Fish were collected from eight Missouri sites: Mississippi River at Saverton, Pool 22, Montrose Lake, the upper 

and lower Blue River, LaBelle Lake, Mozingo Lake, and 

Locust Creek near Pershing State Park. All samples had 

been stored wrapped in aluminum foil in sealed polyethylene bags at -20 degrees Celsius (°C) since collection at the 
2 Determination of Biphenyls, Pesticides, and Flame Retardants in Fillets of Fishes from MDC Monitoring Programs–2007

MDC’s Resource Science Center in Columbia, Missouri, and 

were delivered to the USGS by MDC personnel. Requested 

analyses included total polychlorinated biphenyls (PCBs) and 

PCBs expressed as commercial Aroclor® mixtures historically 

reported in MDC monitoring programs, selected persistent 

organochlorine pesticides (OCPs), and selected polybrominated diphenyl ethers (PBDEs).

Methods

Compositing and Homogenization

Most samples were divided into three five-fish skinned 

fillet composites per site. Fish fillets were prepared by MDC 

personnel, frozen, and transported to the USGS in individually 

labeled bags. In some cases where fish were large, individual 

fillets were analyzed. Samples were stored frozen at -16 oC 

before composite homogenization. Fillets were ground and 

homogenized to prepare samples, then the samples were 

stored in their original sample collection bags at -16 oC before 

analysis. Sample location information was obtained from the 

individually labeled bags.

Preparation

The procedure used to analyze the fish for PCBs, OCPs 

and PBDEs has been previously reported (Hinck and others, 

2006). Briefly, 5-gram (g) portions of fish fillet composite 

samples were removed for analysis, dehydrated by addition 

of anhydrous Na2SO4, and spiked with procedural internal 

standards. Each sample was fortified with 40 nanograms (ng) 

of the following procedural internal standard constituents: 

2,4,5-trichlorobiphenyl (PCB-029), 2,2’,4,4’,6,6’-hexachlorobiphenyl (PCB-155), 2,2’,3,4,4’,5,6,6’-octachlorobiphenyl 

(PCB-204), and deuterated p,p’-DDD (p,p’-DDD-d8) to monitor method recoveries. 

As a trichlorobiphenyl, PCB-029 is representative of the 

more volatile PCBs with one to three chlorine substitutions 

(Cl1 – Cl3); the PCB-155, a hexachlorobiphenyl, of mid-volatility-range PCB congeners with four to six chlorine substitutions (Cl4

 – Cl6); and PCB-204, an octachlorobiphenyl, of 

PCBs with seven to ten chlorine substitutions (Cl7 – Cl10). The 

PCB procedural internal standards also were used to monitor 

recovery of the five less-polar pesticides collected in the first 

silica-gel fraction (SG-1). The latter procedural internal standard, p,p’-DDD-d8

, was used to monitor the recovery of more 

polar pesticides and PBDEs collected in the second silica-gel 

fraction (SG-2). The procedural internal standard compounds 

provide recovery information for each fraction of each sample 

and were used to monitor analyte concentrations.

The Na2SO4-dried fish tissue samples were column 

extracted with dichloromethane, concentrated to 10 milliters (mL), and aliquants (2 percent of the extract) removed 

for lipid analysis determined as total non-volatile residues. 

Aliquants of the extracts (1-g equivalent) were removed for 

PCB analyses. These aliquants were treated by secondary reactive cleanup for removal of biogenic materials and analyzed 

by dual-column gas chromatography with electron-capture 

detection (GC-ECD).

Additional 1.00-g equivalent aliquants of the extracts 

were removed for OCP/PBDE analyses. Sequentially, lowperformance size exclusion chromatography (LP-SEC) and 

high-performance size exclusion chromatography (HP-SEC) 

were used to separate the analytes from residual higher 

molecular weight compounds in the lipophilic extracts. 

Additional biogenic interferences (such as cholesterols) were 

removed by fractionation using basic alumina open-column 

chromatography. Next, the extracts were fractionated using 

layered octadecyl silica/activated silica-gel open-column 

chromatography. The analytes were eluted with mobile phases 

containing increasing solvent polarities: SG-1, hexane eluent, 

collecting about 90 percent of the total PCBs (by mass) and 

six of the targeted OCPs; and SG-2, hexane/methyl t-butyl 

ether (55:45 volume/volume) eluent, collecting the residual 

10 percent of the total PCBs (the less chlorinated congeners), 

the 23 remaining OCPs, and the PBDEs. The fractions were 

concentrated to 1.00 mL for a final concentration of 1 g-equivalents per milliliter.

An operational quality-control (QC) system of checks 

was used to control and assess the measurement quality. This 

system of checks was managed by a quality-assurance system 

that ensures that defined standards of quality are being met 

at stated levels of confidence. The accuracy and precision of 

environmental methods were assessed by utilizing appropriate 

checks for sample preparation and instrumental analysis. The 

appropriate QC sample types were selected based on the applicability to the objectives of this study, and were incorporated 

into the analysis plans, including procedural blanks, replicated 

fish fillet samples, fish tissue matrix blanks, analyte-fortified 

fish tissue matrix blanks, and positive control reference material (Saginaw Bay carp).

Instrumental internal standards (IIS) were added to each 

reactively cleaned-up fraction and final silica-gel fraction, 

with the final volume of each fraction adjusted to 1.0 mL. 

Individual congeners of PCBs, individual congeners of 

PBDEs, and organochlorine pesticides were measured in 

sample fractions by GC-ECD. Potential peaks for analytes 

were matched and identified on one or both gas chromatography (GC) capillary columns with individual standards. Up 

to nine levels of calibration for each analyte were used to 

quantify the targeted congeners. The calibration curves covered a concentration range of 0.01–0.03 to 200 nanograms per 

milliliter (ng/mL).

Method reporting limits (MRLs) for total PCBs were set 

at the average total amounts measured in the procedural blanks 

run with each set based on the lowest calibration standard run 

in the calibration curve [equivalent to 0.1 nanogram per gram 

(ng/g) per congener]. The MRLs were set at 0.1 ng/g for the 

OCPs and 1 ng/g for PBDEs based on procedural background 

amounts and the analysis of low-level calibration standards.
Results and Discussion 3

Quality Control

Recoveries of the procedural internal standards indicative 

of the first silica-gel fraction OCP and PCB analytes (PCB-029, 

-155, and -204) were within QC guidelines of 50 to 125 percent. All samples were between 83 and 114 percent, with the 

exception of the procedural blank sample USGS 021308, 

which incurred preparative losses and recoveries of 60 to 

72 percent for surrogates in the first silica-gel fraction, PCB029, -155, and -204. Recoveries of total PCBs from the fortified negative control matrix (whole bluegill) were 89 percent. 

The recovery of total PCBs from the positive control Saginaw 

Bay carp matrix was 128 percent of the historical average 

(table 2, at the back of this report). The precision of replicate 

analyses (n=3) of sample USGS 41290 (MDC 2007-81-230-1) 

for total PCBs was undetermined because concentrations of 

selected PCB congeners were less than the method reporting 

limits (table 3, at the back of this report). Concentrations of 

total PCBs in the replicate sample were less than 10 ng/g when 

corrected for the procedural blank background PCB concentration. The procedural blank analysis indicated slightly more than 

normal laboratory background levels of total PCBs, of 57 total 

ng per sample. The long term MRL for total PCBs at USGS 

is 21 ng/g. The correlation coefficients of the individual PCB 

congener calibration curves were greater than 0.98, with nearly 

all correlation coefficients greater than 0.98.

The recoveries of the procedural internal standard indicative of the second silica-gel fraction OCP analytes (p,p’-DDD-d8

) 

were within QC guidelines (50 to 125 percent) with recoveries in most samples between 64 to 101 percent, again, with 

the exception of the procedural blank sample USGS 021308 

which incurred preparative losses and recovery of 26 percent 

for the surrogate for the SG-2 fraction, p,p’-DDD-d8. Recoveries of OCPs from the fortified negative control matrix (whole 

bluegill) ranged from 58 to 127 percent, though for most analytes recoveries were more precise, averaging about 99 percent 

(table 3). The recovery of OCPs from the positive control 

Saginaw Bay carp matrix ranged from 61 to 170 percent of 

the historical average for those pesticides with concentrations significantly above the reporting limits, averaging about 

109 percent (table 3). The precision of replicate analyses (n=3) 

of sample USGS 41290 (MDC 2007-81-230-1) for OCPs are 

presented in the table 3. Only 10 OCPs were quantified at 

levels greater than the MRL (0.1 ng/g) in the replicate, and 

7 of these OCPs were less than 1 ng/g and had large relative 

standard deviations (RSDs) (11 to 35 percent). The remaining 

OCPs concentrations ranged from 1 to 2 ng/g and had smaller 

RSDs (6 to 10 percent). Lower values for correlation coefficients were related to slight on-column degradation of active 

contaminants (endrin, p,p’-DDT, and methoxychlor) and the 

precision of methoxychlor was less than most other OCPs as a 

result of PBDE and PCB interferences. The procedural blank 

analyses indicated negligible background levels of OCPs. The 

long term MRL for OCPs at USGS is less than 1 ng/g.

The procedural blank analysis indicated normal laboratory background levels of individual PBDEs ranging from less 

than 0.1 to 3.6 ng/g (PBDE-047), which were considered when 

evaluating the lower PBDE concentration samples (table 4, 

at the back of this report). Recoveries of individual PBDEs 

from the fortified negative control matrix (whole bluegill) 

ranged from 80 to 125 percent, with the exception of PBDE183, which was degraded on-column. The recovery of PBDEs 

from the positive control Saginaw Bay carp matrix was about 

200 percent of the limited historical average (table 4). The 

precision of replicate analyses (n=3) of sample USGS 41290 

(MDC 2007-81-230-1) for PBDEs was undetermined because 

concentrations of selected PBDE congeners were less than the 

MRLs (table 4). Concentrations of total PBDEs in the replicate 

sample were less than 10 ng/g when corrected for the procedural blank background PBDE concentration. The correlation coefficients of the individual PBDE congener calibration 

curves were greater than 0.95.

Results and Discussion

Twenty-six fish fillet composite samples of predator 

and bottom-feeding fish were selected based on the need for 

organic contaminant information and the potentially greater 

risk for human consumption. Samples were collected from 

eight Missouri sites: Mississippi River at Saverton, Pool 22, 

Montrose Lake, the upper and lower Blue River, LaBelle 

Lake, Mozingo Lake, and Locust Creek near Pershing State 

Park. Samples of individual or composited fish fillets are designated by USGS ID and MDC field ID in table 1. The composite samples were prepared and analyzed for PCBs, OCPs, 

and PBDEs by the USGS. The results are presented below. All 

sample values, except procedural blanks, were adjusted for 

recoveries of procedural internal standards (surrogates) and 

corrected for procedural blank background values (tables 2–4). 

Procedural blank values were adjusted for surrogate recoveries 

only (not corrected for procedural blank background values).

Lipids

Lipids in the samples ranged from less than 0.1 to 12 percent (table 1). Largemouth bass and common carp were lowest 

(0.33 to 0.62 percent lipid), lake sturgeon were moderate in 

lipids (1.8 to 2.9 percent lipid), and channel catfish generally 

were the highest (less than 0.1 to 12 percent lipid). No noticeable trend in percent lipids in composited fillets was apparent 

between the 5 sites for the 22 channel catfish samples. The 

small numbers of each species and the lack of representative samples of each species at each site make any definite 

statements about lipid content of these samples difficult. The 

recoveries of procedural internal standards indicated complete 

extraction, indicating that extraction efficiencies for lipids (as 

total dichloromethane-extractable organophilic residues) also 

were excellent. 
4 Determination of Biphenyls, Pesticides, and Flame Retardants in Fillets of Fishes from MDC Monitoring Programs–2007

Total PCBs

Polychlorinated biphenyl results for previous MDC 

monitoring programs were expressed as concentrations of 

Aroclors® 1242, 1248, 1254, and 1260; therefore, the PCB 

congener results from this study were expressed as estimates 

of Aroclors® for compatibility (table 2). Response factors 

for three to four selected PCB congeners per Aroclor® were 

used to determine the fractional contribution of each congener to each Aroclor®: Aroclor® 1242—PCB-018, -019, 

-028, and -031; Aroclor® 1248—PCB-066, -070, and -074; 

Aroclor® 1254—PCB-101, -110, and -118; and Aroclor® 

1260—PCB-153, -170, and -180.

Total concentrations of PCBs in samples ranged from 

background levels of about 60 ng/g to 300 ng/g (table 2). 

Concentrations of PCBs were greatest in channel catfish 

samples from the lower Blue River (110 to 300 ng/g) and in 

lake sturgeon from the Mississippi River-Saverton (Pool 22) 

(78 to 140 ng/g). Expressed as Aroclors®, PCBs consisted 

of a mixture of all four Aroclors® 1242, 1248, 1254, and 

1260, in channel catfish samples from the lower Blue River, 

mean ratio from all samples was 23:25:14:38 percent of each 

Aroclor®, respectively. Samples of channel catfish from the 

upper Blue River were low in PCBs (27 to 33 ng/g), expressed 

as Aroclor® 1260. Expressed as Aroclors®, PCBs primarily 

consisted of Aroclors® 1248, 1254, and 1260 in lake sturgeon 

samples from the Mississippi River, generally in the ratio of 

13:25:62 percent of each Aroclor®, respectively. Negligible 

contributions (less than 10 percent) were estimated from 

Aroclor® 1242. All other samples had concentrations of total 

PCBs that were less than the MRL of 10 ng/g. 

Organochlorine Pesticides

Several OCPs were detected in most samples and were 

present at 10- to 50-fold greater levels in channel catfish 

samples from the lower Blue River (table 3). These OCPs also 

were detected in channel catfish samples from the upper Blue 

River and lake sturgeon from the Mississippi River at Saverton, but at concentrations 2- to 5-fold less than levels from the 

lower Blue River (table 3). Chlordanes (cis/trans-nonachlor, 

cis/trans-chlordane, heptachlor epoxide, and oxychlordane), DDT-related chemicals (p,p’-DDE, and p,p’-DDD), 

and dieldrin constituted the primary classes of pesticides 

detected at elevated concentrations in these samples, ranging from 5 to 75 ng/g. The greatest values of these pesticides 

were detected for a channel catfish composite from the lower 

Blue River USGS 41270 (MDC 2007-12-230-1); other OCP 

concentrations in samples generally were low. Hexachlorocyclohexanes (BHCs: α-BHC, β-BHC, γ-BHC ‘Lindane’, 

δ-BHC), heptachlor, endosulfans (I, II, sulfate), endrin, aldrin, 

pentachlorobenzene, hexachlorobenzene, dacthal, heptachlor, 

and o,p’-DDD, o,p’-DDE, o,p’-DDT, p,p’-DDT, mirex, and 

methoxychlor were not present in any sample at concentrations greater than 5 ng/g.

Selected PBDEs

Total concentrations of PBDEs in samples ranged from 

background levels of about 5 to 86 ng/g, the greatest value 

present in a channel catfish composite sample from the lower 

Blue River (table 4). Concentrations of total PBDEs generally were low, but correlated well with concentrations of total 

PCBs in the fish fillet composites for those sites and samples 

with detectable concentrations. Concentrations of PBDEs, like 

those of total PCBs, were greatest in samples from channel 

catfish from the lower Blue River and were 3- to 6-fold less in 

channel catfish samples from the upper Blue River. Concentrations of PBDEs in lake sturgeon samples from the Mississippi 

River at Saverton were several-fold less than channel catfish 

concentrations from the lower Blue River even though total 

PCBs concentrations were similar in samples from both sites. 

Of the target PBDE congeners, PBDE-047 contributed from 

50 to 80 percent and PBDE-099 contributed from 18 to 40 percent of the total PBDE concentration, respectively. Together, 

these two congeners accounted for 67 to 100 percent of the 

total PBDEs in all samples. The next most significant contribution was from PBDE-100 (less than 1 to about 16 percent). 

Smaller contributions to overall PBDEs were made by PBDE028, with no other congeners contributing to any sample. 

Distinct profiles of PBDEs were observed for the Blue River 

channel catfish samples and for the Mississippi River lake 

sturgeon samples. Channel catfish from the upper and lower 

Blue River exhibited consistent ratios for PBDE-047:-100:-099 

of about 60:10:30. Lake sturgeon from the Mississippi River at 

Saverton exhibited consistent ratios for PBDE-028:-047:-100 

of about 15:75:15. It is not know whether or not the different 

ratios of PBDE congeners reflect differences between species 

(uptake and metabolism), sites (exposure), or both. 

References Cited

Bataille, K.J., 2003, Contaminant concentrations in Missouri 

fish—2001: Missouri Department of Conservation Internal 

Publication. 

Gale, R.W., May, T.W., Orazio, C.E., and McKee, M.J., 2008, 

Determination of polychlorinated biphenyls, selected persistent organochlorine pesticides, and polybrominated flame 

retardants in fillets of fishes from the 2006 Missouri Department of Conservation Monitoring Programs: U.S. Geological Survey Open-File Report 2008–1028, 36 p.

Hinck, J.E., Blazer, V.S., Denslow, N.D., Gross, T.S., Echols, 

K.R., Davis, A.P., May, T.W., Orazio, C.E., Coyle, J.J., and 

Tillitt, D.E., 2006, Biomonitoring of Environmental Status 

arid Trends (BEST) Program: environmental contaminants, 

health indicators, and reproductive biomarkers in fish from 

the Colorado River Basin: U.S. Geological Survey Scientific Investigations Report 2006–5163, 119 p.
References Cited 5

May, T.W., Walther, M.J., Brumbaugh, W.G., 2007, Concentrations of elements in hellbender blood and fish fillets from 

the 2006 Missouri Department of Conservation Monitoring Programs: U.S. Geological Survey Open-File Report 

2007–1299, 18 p.
6 Determination of Biphenyls, Pesticides, and Flame Retardants in Fillets of Fishes from MDC Monitoring Programs–2007
Tables 1–4 

7

Tables 1–4
8 Determination of Biphenyls, Pesticides, and Flame Retardants in Fillets of Fishes from MDC Monitoring Programs–2007

Table 1. Sample description and percent lipid values in Missouri Department of Conservation 2007 fish fillets.

[USGS, U.S. Geological Survey; ID, identification; <, less than; Average, average of replicate analyses; SD, standard deviation; RSD, percent 

relative standard deviation]

USGS ID

number Field ID Fishcommon name

Sample

type Location Lipid(percent)

41267 2007-544-230-1 Channel catfish Fillet Upper Blue River 1.6

41268 2007-544-230-2 Channel catfish Fillet Upper Blue River 1.6

41269 2007-544-230-3 Channel catfish Fillet Upper Blue River 0.9

41270 2007-012-230-1 Channel catfish Fillet Lower Blue River 5.9

41271 2007-012-230-2 Channel catfish Fillet Lower Blue River 2.0

41272 2007-012-230-3 Channel catfish Fillet Lower Blue River 2.2

41273 2007-006-406-1 Largemouth bass Fillet Watkins Mill State Park Lake 0.3

41274 2007-006-230-1 Channel catfish Fillet Watkins Mill State Park Lake 1.5

41275 2007-040-406-1 Largemouth bass Fillet Montrose Lake 0.6

41276 2007-040-070-1 Carp Fillet Montrose Lake 0.5

41277 2007-315-010-1 Lake sturgeon Fillet Mississippi River-Saverton Pool 22 2.9

41278 2007-315-010-2 Lake sturgeon Fillet Mississippi River-Saverton Pool 22 2.8

41279 2007-315-010-3 Lake sturgeon Fillet Mississippi River-Saverton Pool 22 1.8

41280 2007-222-230-1 Channel catfish Fillet LaBelle Lake < 0.1

41281 2007-222-230-2 Channel catfish Fillet LaBelle Lake 0.3

41282 2007-222-230-3 Channel catfish Fillet LaBelle Lake 2.7

41283 2007-222-230-4 Channel catfish Fillet LaBelle Lake 0.9

41284 2007-222-230-5 Channel catfish Fillet LaBelle Lake 4.3

41285 2007-651-230-1 Channel catfish Fillet Mozingo Lake 1.4

41286 2007-651-230-2 Channel catfish Fillet Mozingo Lake 1.8

41287 2007-651-230-3 Channel catfish Fillet Mozingo Lake 3.1

41288 2007-651-230-4 Channel catfish Fillet Mozingo Lake 12

41289 2007-651-230-5 Channel catfish Fillet Mozingo Lake 6.7

41290-1 2007-081-230-1 Channel catfish Fillet Locust Creek near Pershing State Park 3.2

41290-2 2007-081-230-1 Channel catfish Fillet Locust Creek near Pershing State Park 3.4

41290-3 2007-081-230-1 Channel catfish Fillet Locust Creek near Pershing State Park 3.5

Average: 3.4

SD: .15

RSD: 5

41291 2007-081-230-2 Channel catfish Fillet Locust Creek near Pershing State Park 1.9

41292 2007-081-230-3 Channel catfish Fillet Locust Creek near Pershing State Park 2.3

PB-0213086 Procedural blank < 0.1

MB-021408 Matrix blank Control bluegill 654C Whole 1.2

MS-OC-021308 Matrix spike - OCs Control bluegill 654C Whole 1.0

MS-PCB/PBDE-021308 Matrix spike - PCBs/PBDEs Control bluegill 654C Whole 1.0

PC-021408 Positive control Saginaw carp 6806 Whole 16

Baseline PC data: Positive control Saginaw carp 6806 Whole Percent lipid range (1994 – 2008) 12 – 16
Table 2. Total polychlorinated biphenyl and estimated Aroclor® concentrations and Aroclor® percentages in Missouri Department of Conservation 2fillets.—Continued[USGS, U.S. Geological Survey; ID, identification; PCB, polychlorinated biphenyl; ng/g, nanogram per gram; <, less than the reporting limit; --, not reported; Average, average standard deviation; RSD, percent relative standard deviation; All values are adjusted for surrogate recoveries and procedural blank sample background values]

USGS ID

number Field ID

Measured1

 total PCBs 

(ng/g)

Aroclor 12422 Aroclor 12483 Aroclor 12544 Aroclor 12605 

(ng/g) (percent) (ng/g) (percent) (ng/g) (percent) (ng/g) (percen

41267 2007-544-230-1 37 < 10 -- 11 21 < 10 -- 33 64 41268 2007-544-230-2 11 < 10 -- < 10 -- < 10 -- 33 100 41269 2007-544-230-3 < 10 < 10 -- < 10 -- < 10 -- 27 100 41270 2007-012-230-1 300 95 28 82 24 49 14 110 32 41271 2007-012-230-2 110 31 20 37 24 19 13 65 43 41272 2007-012-230-3 150 43 22 49 26 26 14 73 38 41273 2007-006-406-1 < 10 < 10 -- < 10 -- < 10 -- < 10 -- 41274 2007-006-230-1 < 10 < 10 -- < 10 -- < 10 -- < 10 -- 41275 2007-040-406-1 < 10 < 10 -- < 10 -- < 10 -- < 10 -- 41276 2007-040-070-1 < 10 < 10 -- < 10 -- < 10 -- < 10 -- 41277 2007-315-010-1 96 < 10 -- 18 13 35 26 82 60 41278 2007-315-010-2 78 < 10 -- 14 13 25 23 68 63 41279 2007-315-010-3 140 < 10 -- 21 12 44 25 110 62 41280 2007-222-230-1 < 10 < 10 -- < 10 -- < 10 -- < 10 -- 41281 2007-222-230-2 < 10 < 10 -- < 10 -- < 10 -- < 10 -- 41282 2007-222-230-3 < 10 < 10 -- < 10 -- < 10 -- < 10 -- 41283 2007-222-230-4 < 10 < 10 -- < 10 -- < 10 -- < 10 -- 41284 2007-222-230-5 < 10 < 10 -- < 10 -- < 10 -- < 10 -- 41285 2007-651-230-1 < 10 < 10 -- < 10 -- < 10 -- < 10 -- 41286 2007-651-230-2 < 10 < 10 -- < 10 -- < 10 -- < 10 -- 41287 2007-651-230-3 < 10 < 10 -- < 10 -- < 10 -- < 10 -- 41288 2007-651-230-4 < 10 < 10 -- < 10 -- < 10 -- < 10 -- 41289 2007-651-230-5 < 10 < 10 -- < 10 -- < 10 -- < 10 -- 41290-1 2007-081-230-1 < 10 < 10 -- < 10 -- < 10 -- < 10 -- 41290-2 2007-081-230-1 < 10 < 10 -- < 10 -- < 10 -- < 10 -- 41290-3 2007-081-230-1 < 10 < 10 -- < 10 -- < 10 -- < 10 -- 

Average: < 10 < 10 -- < 10 -- < 10 -- < 10 -- SD: -- -- -- -- -- -- -- -- -- RSD: -- -- -- -- -- -- -- -- -- 
Table 2. Total polychlorinated biphenyl and estimated Aroclor® concentrations and Aroclor® percentages in Missouri Department of Conservation 2fillets.—Continued [USGS, U.S. Geological Survey; ID, identification; PCB, polychlorinated biphenyl; ng/g, nanogram per gram; <, less than the reporting limit; --, not reported; Average, average standard deviation; RSD, percent relative standard deviation; All values are adjusted for surrogate recoveries and procedural blank sample background values] USGS ID number Field ID Measured1 total PCBs (ng/g) 

Aroclor 12422 Aroclor 12483 Aroclor 12544 Aroclor 12605 

(ng/g) (percent) (ng/g) (percent) (ng/g) (percent) (ng/g) (percen

41291 2007-081-230-2 < 10 < 10 -- < 10 -- < 10 -- < 10 -- 41292 2007-081-230-3 < 10 < 10 -- < 10 -- < 10 -- < 10 -- PB-0213086 Procedural blank7 57 2.7 -- 2.4 -- 7.6 -- 4.3 -- MB-021408 Matrix blank 16 < 10 -- < 10 -- < 10 -- < 10 -- MS-PCB/PBDE-021308 Matrix spike - PCBs/ PBDEs 890 230 19 260 22 280 23 440 36 PC-021408 Positive control Saginaw carp 6806 8,300 780 6 4,400 35 4,500 36 2,900 23 Baseline PC data: Positive control Saginaw carp 6806 6,500 530 8 2,400 38 2,400 38 1,100 17 Commercial PCB mixtures used as Reference Materials Aroclor® 1242 (n=2) 850 620 62 330 33 51 5 < 10 -- Aroclor® 1248 (n=2) 920 340 29 600 52 170 15 36 3 Aroclor® 1254 (n=2) 890 12 1 150 13 600 52 390 34 Aroclor® 1260 (n=2) 890 < 10 -- < 10 -- 150 12 1,100 85 

1 Summation of individual congeners. 2 Congeners summed for Aroclor ® 1242 estimation (PCB-018, -019, -028, -031). 3 Congeners summed for Aroclor ® 1248 estimation (PCB-066, -070, -074). 4 Congeners summed for Aroclor ® 1254 estimation (PCB-101, -110, -118). 5 Congeners summed for Aroclor ® 1260 estimation (PCB-153, -170, -180). 6 Total Aroclor ® (sum of Aroclors ® 1242, 1248, 1254, 1260) compared to the sum of individual congeners and reported as a percentage. 7 Procedural blank values are reported as total amounts (not concentrations), are adjusted for surrogate recovery, and are not corrected for background values.
Table 3. Organochlorine pesticide concentrations in Missouri Department of Conservation 2007 fish fillets.—Continued[USGS, U.S. Geological Survey; ID, identification; ng/g, nanogram per gram; BHC, benzene hexachloride; HCH, hexachlorocyclohexane; <, less than the reporting limit; --, noAverage, average of replicate analyses; SD, standard deviation; RSD, percent relative standard deviation; All values are adjusted for surrogate recoveries and procedural blank sground values]

USGS ID

number Field ID

Pentachlorobenzene

(ng/g)

Hexachlorobenzene

(ng/g)

Pentachloroanisole

(ng/g)

alpha-BHC

(α-HCH)

(ng/g)

beta-BHC

(β-HCH)

(ng/g)

Lindane

(γ-HCH)

(ng/g)

delta-BH

(δ-HCH

(ng/g)

41267 2007-544-230-1 < 0.1 0.2 2.0 < 0.1 < 0.1 < 0.1 < 0.1 41268 2007-544-230-2 < 0.1 0.2 2.2 < 0.1 < 0.1 < 0.1 < 0.1 41269 2007-544-230-3 < 0.1 < 0.1 1.4 < 0.1 < 0.1 < 0.1 < 0.1 41270 2007-012-230-1 0.3 1.5 3.8 < 0.1 < 0.1 < 0.1 < 0.1 41271 2007-012-230-2 0.1 0.5 1.7 < 0.1 < 0.1 < 0.1 < 0.1 41272 2007-012-230-3 0.1 0.6 1.9 < 0.1 < 0.1 < 0.1 < 0.1 41273 2007-006-406-1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 41274 2007-006-230-1 < 0.1 < 0.1 0.6 < 0.1 < 0.1 < 0.1 < 0.1 41275 2007-040-406-1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 41276 2007-040-070-1 < 0.1 < 0.1 0.3 < 0.1 < 0.1 < 0.1 < 0.1 41277 2007-315-010-1 < 0.1 0.2 0.6 < 0.1 < 0.1 < 0.1 < 0.1 41278 2007-315-010-2 < 0.1 0.3 0.8 < 0.1 < 0.1 < 0.1 < 0.1 41279 2007-315-010-3 < 0.1 < 0.1 0.7 < 0.1 < 0.1 < 0.1 < 0.1 41280 2007-222-230-1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 0.2 < 0.1 41281 2007-222-230-2 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 0.2 < 0.1 41282 2007-222-230-3 < 0.1 < 0.1 0.4 < 0.1 < 0.1 0.4 < 0.1 41283 2007-222-230-4 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 0.2 < 0.1 41284 2007-222-230-5 < 0.1 0.1 0.4 < 0.1 < 0.1 0.2 < 0.1 41285 2007-651-230-1 < 0.1 < 0.1 0.2 < 0.1 < 0.1 0.2 < 0.1 41286 2007-651-230-2 < 0.1 0.1 0.2 < 0.1 < 0.1 0.3 < 0.1 41287 2007-651-230-3 < 0.1 < 0.1 0.5 < 0.1 < 0.1 0.5 < 0.1 41288 2007-651-230-4 < 0.1 0.3 0.8 < 0.1 < 0.1 0.4 < 0.1 41289 2007-651-230-5 < 0.1 0.3 0.9 < 0.1 < 0.1 0.5 < 0.1 
Table 3. Organochlorine pesticide concentrations in Missouri Department of Conservation 2007 fish fillets.—Continued [USGS, U.S. Geological Survey; ID, identification; ng/g, nanogram per gram; BHC, benzene hexachloride; HCH, hexachlorocyclohexane; <, less than the reporting limit; --, noAverage, average of replicate analyses; SD, standard deviation; RSD, percent relative standard deviation; All values are adjusted for surrogate recoveries and procedural blank sground values] USGS ID number Field ID Pentachloro- benzene (ng/g) Hexachloro- benzene (ng/g) Pentachloro- anisole (ng/g) alpha-BHC (α-HCH) (ng/g) beta-BHC (β-HCH) (ng/g) Lindane (γ-HCH) (ng/g) delta-BH

(δ-HCH

(ng/g) 

41290-1 2007-081-230-1 < 0.1 0.1 0.8 < 0.1 < 0.1 0.4 < 0.1 41290-2 2007-081-230-1 < 0.1 0.2 0.8 < 0.1 < 0.1 0.4 < 0.1 41290-3 2007-081-230-1 < 0.1 < 0.1 0.4 < 0.1 < 0.1 0.5 < 0.1 

Average: < 0.1 0.1 0.7 < 0.1 < 0.1 0.4 < 0.1 SD: -- -- 0.2 -- -- 0.06 -- RSD: -- -- 35 -- -- 13 -- 

41291 2007-081-230-2 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 0.3 < 0.1 41292 2007-081-230-3 < 0.1 < 0.1 0.6 < 0.1 < 0.1 0.2 < 0.1 PB-0213086 Procedural blank7 < 0.1 0.1 0.2 < 0.1 < 0.1 < 0.1 < 0.1 MB-021408 Matrix blank < 0.1 0.3 0.2 0.2 < 0.1 0.3 < 0.1 MS-OCP-021408 Matrix spike - OCPs 36 39 50 43 40 43 40 PC-021408 Positive control Saginaw carp 6806 6.4 15 2.0 4.0 < 0.1 2.4 < 0.1 Baseline PC data: Positive control Saginaw carp 6806 -- 10 3.3 5.1 2.9 3 1 
Table 3. Organochlorine pesticide concentrations in Missouri Department of Conservation 2007 fish fillets.—Continued[USGS, U.S. Geological Survey; ID, identification; ng/g, nanogram per gram; BHC, benzene hexachloride; HCH, hexachlorocyclohexane; <, less than the reporting limit; --, noreplicate analyses; SD, standard deviation; RSD, percent relative standard deviation; All values are adjusted for surrogate recoveries and procedural blank sample background v

USGS ID

number Field ID

Heptachlor

epoxide

(ng/g)

Aldrin

(ng/g)

Dacthal

(ng/g)

Oxychlordane

(ng/g)

cis-Chlordane

(ng/g)

trans-Chlordane

(ng/g)

cis-N

(n

41267 2007-544-230-1 0.6 < 0.1 < 0.1 1.1 4.8 2.9 41268 2007-544-230-2 0.6 < 0.1 < 0.1 0.9 3.0 2.0 41269 2007-544-230-3 0.3 < 0.1 < 0.1 0.5 2.1 1.2 41270 2007-012-230-1 15 < 0.1 0.2 5.0 55 40 41271 2007-012-230-2 6.6 < 0.1 < 0.1 2.4 24 17 41272 2007-012-230-3 8.9 < 0.1 < 0.1 3.2 35 24 41273 2007-006-406-1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 41274 2007-006-230-1 < 0.1 < 0.1 < 0.1 0.1 0.3 0.1 41275 2007-040-406-1 < 0.1 < 0.1 < 0.1 < 0.1 0.1 < 0.1 41276 2007-040-070-1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 41277 2007-315-010-1 1.1 < 0.1 < 0.1 1.0 3.0 2.1 41278 2007-315-010-2 1.1 < 0.1 < 0.1 0.9 4.3 2.5 41279 2007-315-010-3 0.6 < 0.1 < 0.1 1.1 3.0 2.4 41280 2007-222-230-1 < 0.1 < 0.1 < 0.1 < 0.1 0.1 < 0.1 41281 2007-222-230-2 < 0.1 < 0.1 < 0.1 < 0.1 0.1 < 0.1 41282 2007-222-230-3 0.2 < 0.1 < 0.1 0.4 1.2 1.2 41283 2007-222-230-4 < 0.1 < 0.1 < 0.1 0.2 0.8 0.5 41284 2007-222-230-5 0.4 < 0.1 0.1 0.7 1.5 1.4 41285 2007-651-230-1 < 0.1 < 0.1 < 0.1 0.2 0.4 0.2 41286 2007-651-230-2 0.2 < 0.1 < 0.1 0.3 1.0 0.4 41287 2007-651-230-3 0.3 < 0.1 0.1 0.4 0.9 0.4 41288 2007-651-230-4 0.9 < 0.1 < 0.1 0.5 0.9 0.7 41289 2007-651-230-5 0.7 < 0.1 0.4 0.4 1.2 0.8 
Table 3. Organochlorine pesticide concentrations in Missouri Department of Conservation 2007 fish fillets.—Continued [USGS, U.S. Geological Survey; ID, identification; ng/g, nanogram per gram; BHC, benzene hexachloride; HCH, hexachlorocyclohexane; <, less than the reporting limit; --, noreplicate analyses; SD, standard deviation; RSD, percent relative standard deviation; All values are adjusted for surrogate recoveries and procedural blank sample background v

USGS ID number Field ID Heptachlor epoxide (ng/g) Aldrin (ng/g) Dacthal (ng/g) Oxychlordane (ng/g) cis-Chlordane (ng/g) trans-Chlordane (ng/g) cis-N

(n

41290-1 2007-081-230-1 0.5 < 0.1 < 0.1 0.3 0.6 0.3 41290-2 2007-081-230-1 0.6 < 0.1 < 0.1 0.3 0.5 0.2 41290-3 2007-081-230-1 0.5 < 0.1 < 0.1 0.3 0.5 0.3 

Average: 0.5 < 0.1 < 0.1 0.3 0.5 0.2 SD: 0.06 -- -- -- 0.06 0.06 RSD: 11 -- -- -- 11 22 

41291 2007-081-230-2 0.2 < 0.1 < 0.1 0.2 0.3 0.1 41292 2007-081-230-3 0.7 < 0.1 < 0.1 0.4 0.4 0.3 PB-0213086 Procedural blank7 0.1 < 0.1 0.3 < 0.1 0.1 0.1 < MB-021408 Matrix blank 0.4 < 0.1 0.5 0.9 0.4 < 0.1 MS-OCP-021408 Matrix spike - OCPs 47 37 56 48 49 48 PC-021408 Positive control Saginaw carp 6806 4.1 < 0.1 3.9 3.5 26 12 Baseline PC data: Positive control Saginaw carp 6806 4 < 0.1 4.4 4 21 10 
Table 3. Organochlorine pesticide concentrations in Missouri Department of Conservation 2007 fish fillets.—Continued[USGS, U.S. Geological Survey; ID, identification; ng/g, nanogram per gram; BHC, benzene hexachloride; HCH, hexachlorocyclohexane; <, less than the reporting limit; --, noaverage of replicate analyses; SD, standard deviation; RSD, percent relative standard deviation; All values are adjusted for surrogate recoveries and procedural blank sample ba

USGS ID

Number Field ID o,p’-DDE (ng/g) o,p’-DDD (ng/g) o,p’-DDT (ng/g) p,p’-DDE (ng/g) p,p’-DDD (ng/g) p,p’-DDT (ng/g) Endosulfan I (ng/g) Endos

(ng

41267 2007-544-230-1 0.5 < 0.1 0.2 8.2 2.4 < 0.1 < 0.1 < 0.41268 2007-544-230-2 < 0.1 < 0.1 < 0.1 5.5 0.7 < 0.1 < 0.1 < 0.41269 2007-544-230-3 0.2 < 0.1 < 0.1 3.5 0.4 < 0.1 < 0.1 < 0.41270 2007-012-230-1 0.3 5.2 < 0.1 43 24 2.4 < 0.1 < 0.41271 2007-012-230-2 < 0.1 2.2 < 0.1 17 11 0.4 < 0.1 < 0.41272 2007-012-230-3 0.1 3.1 < 0.1 24 14 0.4 < 0.1 < 0.41273 2007-006-406-1 < 0.1 < 0.1 < 0.1 0.3 < 0.1 < 0.1 < 0.1 < 0.41274 2007-006-230-1 0.4 < 0.1 < 0.1 3.2 0.2 < 0.1 < 0.1 < 0.41275 2007-040-406-1 < 0.1 < 0.1 < 0.1 0.7 < 0.1 < 0.1 < 0.1 < 0.41276 2007-040-070-1 < 0.1 < 0.1 < 0.1 0.5 < 0.1 < 0.1 < 0.1 < 0.41277 2007-315-010-1 0.2 0.6 < 0.1 12 1.8 < 0.1 < 0.1 < 0.41278 2007-315-010-2 0.3 0.6 < 0.1 13 1.5 < 0.1 < 0.1 < 0.41279 2007-315-010-3 0.5 < 0.1 < 0.1 17 2.3 < 0.1 < 0.1 < 0.41280 2007-222-230-1 < 0.1 < 0.1 < 0.1 0.7 0.2 < 0.1 < 0.1 < 0.41281 2007-222-230-2 < 0.1 < 0.1 < 0.1 < 0.1 0.1 < 0.1 < 0.1 < 0.41282 2007-222-230-3 < 0.1 < 0.1 < 0.1 2.2 0.3 < 0.1 < 0.1 < 0.41283 2007-222-230-4 < 0.1 < 0.1 < 0.1 2.7 0.3 < 0.1 < 0.1 < 0.41284 2007-222-230-5 < 0.1 < 0.1 < 0.1 2.9 0.3 < 0.1 < 0.1 < 0.41285 2007-651-230-1 < 0.1 < 0.1 < 0.1 2.2 0.2 < 0.1 < 0.1 < 0.41286 2007-651-230-2 < 0.1 < 0.1 < 0.1 3.0 0.4 < 0.1 < 0.1 < 0.41287 2007-651-230-3 < 0.1 0.2 < 0.1 4.8 0.6 < 0.1 < 0.1 < 0.41288 2007-651-230-4 < 0.1 0.3 < 0.1 2.6 0.5 < 0.1 < 0.1 < 0.41289 2007-651-230-5 < 0.1 < 0.1 < 0.1 2.6 0.4 < 0.1 < 0.1 < 0.
Table 3. Organochlorine pesticide concentrations in Missouri Department of Conservation 2007 fish fillets.—Continued [USGS, U.S. Geological Survey; ID, identification; ng/g, nanogram per gram; BHC, benzene hexachloride; HCH, hexachlorocyclohexane; <, less than the reporting limit; --, noaverage of replicate analyses; SD, standard deviation; RSD, percent relative standard deviation; All values are adjusted for surrogate recoveries and procedural blank sample ba

USGS ID Number Field ID o,p’-DDE (ng/g) o,p’-DDD (ng/g) o,p’-DDT (ng/g) p,p’-DDE (ng/g) p,p’-DDD (ng/g) p,p’-DDT (ng/g) Endosulfan I (ng/g) Endos

(ng

41290-1 2007-081-230-1 < 0.1 0.4 < 0.1 1.8 0.3 < 0.1 0.1 < 041290-2 2007-081-230-1 < 0.1 0.5 < 0.1 2.0 0.2 < 0.1 < 0.1 < 041290-3 2007-081-230-1 < 0.1 < 0.1 < 0.1 2.1 0.3 < 0.1 < 0.1 < 0

Average: < 0.1 0.3 < 0.1 1.9 0.3 < 0.1 < 0.1 < 0SD: -- 0.24 -- 0.15 0.06 -- -- -

RSD: -- 75 -- 7.8 22 -- -- -

41291 2007-081-230-2 < 0.1 0.2 < 0.1 0.9 0.2 < 0.1 < 0.1 < 041292 2007-081-230-3 < 0.1 < 0.1 < 0.1 1.9 0.3 < 0.1 < 0.1 < 0PB-0213086 Procedural blank7 0.1 < 0.1 < 0.1 0.4 < 0.1 < 0.1 < 0.1 < 0MB-021408 Matrix blank < 0.1 < 0.1 < 0.1 4.0 0.4 < 0.1 < 0.1 < 0MS-OCP-021408 Matrix spike - OCPs 46 46 33 57 36 27 46 39 PC-021408 Positive control Saginaw carp 6806 6.9 57 0.9 590 260 0.9 3.1 < 0Baseline PC data: Positive control Saginaw carp 6806 9.1 48 0.21 350 240 4.6 12 1
Table 3. Organochlorine pesticide concentrations in Missouri Department of Conservation 2007 fish fillets.—Continued[USGS, U.S. Geological Survey; ID, identification; ng/g, nanogram per gram; BHC, benzene hexachloride; HCH, hexachlorocyclohexane; <, less than the reporting limit; --, noaverage of replicate analyses; SD, standard deviation; RSD, percent relative standard deviation; All values are adjusted for surrogate recoveries and procedural blank sample ba

USGS ID

Number Field ID Dieldrin (ng/g) Mirex (ng/g) Methoxychlor (ng/g) Total BHCs1 (ng/g) Total endrin2 (ng/g) Total DDT Series3 (ng/g) Total chlordanes4 (ng/g) 

is

(

41267 2007-544-230-1 2.0 < 0.1 < 0.1 < 1 < 1 11 19 41268 2007-544-230-2 2.1 < 0.1 < 0.1 < 1 < 1 6.2 12 41269 2007-544-230-3 1.3 < 0.1 < 0.1 < 1 < 1 4.1 8.5 41270 2007-012-230-1 75 < 0.1 < 0.1 < 1 < 1 75 160 41271 2007-012-230-2 28 < 0.1 < 0.1 < 1 < 1 31 69 41272 2007-012-230-3 44 < 0.1 < 0.1 < 1 < 1 42 98 41273 2007-006-406-1 < 0.1 < 0.1 < 0.1 < 1 < 1 < 1 < 1 41274 2007-006-230-1 0.3 < 0.1 < 0.1 < 1 < 1 3.8 1.8 41275 2007-040-406-1 < 0.1 < 0.1 < 0.1 < 1 < 1 < 1 < 1 41276 2007-040-070-1 < 0.1 < 0.1 < 0.1 < 1 < 1 < 1 < 1 41277 2007-315-010-1 4.5 < 0.1 < 0.1 < 1 < 1 15 15 41278 2007-315-010-2 4.1 0.1 < 0.1 < 1 < 1 15 18 41279 2007-315-010-3 2.7 0.1 < 0.1 < 1 < 1 20 17 41280 2007-222-230-1 < 0.1 < 0.1 < 0.1 < 1 < 1 < 1 < 1 41281 2007-222-230-2 < 0.1 < 0.1 < 0.1 < 1 < 1 < 1 < 1 41282 2007-222-230-3 0.6 < 0.1 < 0.1 < 1 < 1 2.5 6.3 41283 2007-222-230-4 < 0.1 < 0.1 < 0.1 < 1 < 1 3.0 3.8 41284 2007-222-230-5 0.9 < 0.1 < 0.1 < 1 < 1 3.2 7.7 41285 2007-651-230-1 0.9 < 0.1 < 0.1 < 1 < 1 2.4 2.1 41286 2007-651-230-2 2.2 < 0.1 < 0.1 < 1 < 1 3.4 4.2 41287 2007-651-230-3 2.7 < 0.1 < 0.1 < 1 < 1 5.6 4.9 41288 2007-651-230-4 7.8 < 0.1 < 0.1 < 1 < 1 3.4 5.1 41289 2007-651-230-5 6.8 < 0.1 < 0.1 < 1 < 1 3.0 5.2 
Table 3. Organochlorine pesticide concentrations in Missouri Department of Conservation 2007 fish fillets.—Continued [USGS, U.S. Geological Survey; ID, identification; ng/g, nanogram per gram; BHC, benzene hexachloride; HCH, hexachlorocyclohexane; <, less than the reporting limit; --, noaverage of replicate analyses; SD, standard deviation; RSD, percent relative standard deviation; All values are adjusted for surrogate recoveries and procedural blank sample ba

USGS ID Number Field ID Dieldrin (ng/g) Mirex (ng/g) Methoxychlor (ng/g) Total BHCs1 (ng/g) Total endrin2 (ng/g) Total DDT Series3 (ng/g) Total chlordanes4 (ng/g) 

is

(

41290-1 2007-081-230-1 1.4 < 0.1 < 0.1 < 1 < 1 2.5 3.0 41290-2 2007-081-230-1 1.6 < 0.1 < 0.1 < 1 < 1 2.7 2.8 41290-3 2007-081-230-1 1.7 < 0.1 < 0.1 < 1 < 1 2.4 2.9 

Average: 1.6 < 0.1 < 0.1 < 1 < 1 2.5 2.9 SD: 0.15 -- -- -- -- 0.15 0.1 RSD: 9.8 -- -- -- -- 6 3.4 

41291 2007-081-230-2 0.8 < 0.1 < 0.1 < 1 < 1 1.3 1.7 41292 2007-081-230-3 1.9 < 0.1 < 0.1 < 1 < 1 2.2 3.1 PB-0213086 Procedural blank7 0.6 < 0.1 < 0.1 < 1 < 1 < 1 < 1 MB-021408 Matrix blank 4.5 < 0.1 < 0.1 < 1 < 1 4.4 6.3 MS-OCP-021408 Matrix spike - OCPs 51 45 21 170 34 250 340 PC-021408 Positive control Saginaw carp 6806 18 3.6 < 0.1 6.4 3.4 920 86 Baseline PC data: Positive control Saginaw carp 6806 16 1.9 27 12 2.8 650 97 

1 Summation of α-HCH, β-HCH, γ-HCH, δ-HCH. 2 Summation of endrin, endrin aldehyde, endrin ketone. 3 Summation of o,p’-DDD, o,p’-DDE, o,p’-DDT, p,p’-DDD, p,p’-DDE, p,p’-DDT. 4 Summation of cis-chlordane, trans-chlordane, cis-nonachlor, trans-nonachlor, oxychlordane, heptachlor, heptachlor epoxide, methoxychlor. 5 Summation of aldrin, dieldrin, total endrin. 6 Summation of endosulfan I, endosulfan II, endosulfan sulfate. 7 Procedural blank values are reported as total amounts (not concentrations), are adjusted for surrogate recovery, and are not corrected for background values.
Tables 1–4 19

Table 4. Polybrominated diphenyl ethers concentrations and percentages in Missouri Department of Conservation 2007 fish 

fillets.

[USGS, U.S. Geological Survey; ID, identification; PBDE, polybrominated diphenyl ether; ng/g, nanogram per gram; <, less than the reporting limit; --, not 

reported; Average, average of replicate analyses; SD, standard deviation; RSD, percent relative standard deviation; All values are adjusted for surrogate 

recoveries and procedural blank sample background values]

USGS ID

number Field ID

PBDE-028 PBDE-047 PBDE-066 PBDE-100 PBDE-099

(ng/g) (percent) (ng/g) (percent) (ng/g) (percent) (ng/g) (percent) (ng/g) (percent)

41267 2007-544-230-1 < 1 -- 21 61 < 1 -- 3.7 11 9.9 29

41268 2007-544-230-2 < 1 -- 12 69 < 1 -- 1.3 7 4.1 24

41269 2007-544-230-3 < 1 -- 10 74 < 1 -- 1.2 9 2.4 18

41270 2007-012-230-1 < 1 -- 44 51 < 1 -- 8.6 10 33 39

41271 2007-012-230-2 < 1 -- 13 53 < 1 -- 2.5 10 9.1 37

41272 2007-012-230-3 < 1 -- 15 49 < 1 -- 3.8 12 12 39

41273 2007-006-406-1 < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

41274 2007-006-230-1 < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

41275 2007-040-406-1 < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

41276 2007-040-070-1 < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

41277 2007-315-010-1 < 1 5.8 84 < 1 -- 1.1 16 < 1 --

41278 2007-315-010-2 2 12 11 74 < 1 -- 2.1 14 < 1 --

41279 2007-315-010-3 1.9 17 7.3 67 < 1 -- 1.7 16 < 1 --

41280 2007-222-230-1 < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

41281 2007-222-230-2 < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

41282 2007-222-230-3 < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

41283 2007-222-230-4 < 1 -- < 1 -- < 1 -- < 1 -- 1.5 100

41284 2007-222-230-5 < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

41285 2007-651-230-1 < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

41286 2007-651-230-2 < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

41287 2007-651-230-3 < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

41288 2007-651-230-4 < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

41289 2007-651-230-5 < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

41290-1 2007-081-230-1 < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

41290-2 2007-081-230-1 < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

41290-3 2007-081-230-1 < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

Average: < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

SD: -- -- -- -- -- -- -- -- -- --

RSD: -- -- -- -- -- -- -- -- -- --

41291 2007-081-230-2 < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

41292 2007-081-230-3 < 1 -- < 1 -- < 1 -- < 1 -- < 1 --

PB-0213086 Procedural blank2 0.2 -- 3.6 -- 0.2 -- 0.2 -- 1.2 --

MB-021408 Matrix blank < 1 -- < 1 -- < 1 -- < 1 -- 1.8 100

MS-PCB/PBDE-021308 Matrix spike - PCBs/PBDEs 25 15 21 12 24 14 23 14 24 14

PC-021408 Positive control Saginaw 

carp 6806

< 1 -- 1.7 41 < 1 -- 1.3 32 1.1 27

Baseline PC data: Positive control Saginaw 

carp 6806

-- -- 5.1 53 -- -- 0.8 8 2.5 26
20 Determination of Biphenyls, Pesticides, and Flame Retardants in Fillets of Fishes from MDC Monitoring Programs–2007

Table 4. Polybrominated diphenyl ethers concentrations and percentages in Missouri Department of Conservation 2007 fish 

fillets.—Continued

[USGS, U.S. Geological Survey; ID, identification; PBDE, polybrominated diphenyl ether; ng/g, nanogram per gram; <, less than the reporting limit; --, not 

reported; Average, average of replicate analyses; SD, standard deviation; RSD, percent relative standard deviation; All values are adjusted for surrogate 

recoveries and procedural blank sample background values]

USGS ID number Field ID

PBDE-085 PBDE-154 PBDE-153 PBDE-1831 Total PBDE

(ng/g) (percent) (ng/g) (percent) (ng/g) (percent) (ng/g) (percent) (ng/g)

41267 2007-544-230-1 < 1 -- < 1 -- < 1 -- < 1 -- 35

41268 2007-544-230-2 < 1 -- < 1 -- < 1 -- < 1 -- 17

41269 2007-544-230-3 < 1 -- < 1 -- < 1 -- < 1 -- 14

41270 2007-012-230-1 < 1 -- < 1 -- < 1 -- < 1 -- 86

41271 2007-012-230-2 < 1 -- < 1 -- < 1 -- < 1 -- 25

41272 2007-012-230-3 < 1 -- < 1 -- < 1 -- < 1 -- 31

41273 2007-006-406-1 < 1 -- < 1 -- < 1 -- < 1 -- < 10

41274 2007-006-230-1 < 1 -- < 1 -- < 1 -- < 1 -- < 10

41275 2007-040-406-1 < 1 -- < 1 -- < 1 -- < 1 -- < 10

41276 2007-040-070-1 < 1 -- < 1 -- < 1 -- < 1 -- < 10

41277 2007-315-010-1 < 1 -- < 1 -- < 1 -- < 1 -- 7

41278 2007-315-010-2 < 1 -- < 1 -- < 1 -- < 1 -- 15

41279 2007-315-010-3 < 1 -- < 1 -- < 1 -- < 1 -- 11

41280 2007-222-230-1 < 1 -- < 1 -- < 1 -- < 1 -- < 10

41281 2007-222-230-2 < 1 -- < 1 -- < 1 -- < 1 -- < 10

41282 2007-222-230-3 < 1 -- < 1 -- < 1 -- < 1 -- < 10

41283 2007-222-230-4 < 1 -- < 1 -- < 1 -- < 1 -- 2

41284 2007-222-230-5 < 1 -- < 1 -- < 1 -- < 1 -- < 10

41285 2007-651-230-1 < 1 -- < 1 -- < 1 -- < 1 -- < 10

41286 2007-651-230-2 < 1 -- < 1 -- < 1 -- < 1 -- < 10

41287 2007-651-230-3 < 1 -- < 1 -- < 1 -- < 1 -- < 10

41288 2007-651-230-4 < 1 -- < 1 -- < 1 -- < 1 -- < 10

41289 2007-651-230-5 < 1 -- < 1 -- < 1 -- < 1 -- < 10

41290-1 2007-081-230-1 < 1 -- < 1 -- < 1 -- < 1 -- < 10

41290-2 2007-081-230-1 < 1 -- < 1 -- < 1 -- < 1 -- < 10

41290-3 2007-081-230-1 < 1 -- < 1 -- < 1 -- < 1 -- < 10

Average: < 1 -- < 1 -- < 1 -- < 1 -- < 10

SD: -- -- -- -- -- -- -- -- --

RSD: -- -- -- -- -- -- -- -- --

41291 2007-081-230-2 < 1 -- < 1 -- < 1 -- < 1 -- < 10

41292 2007-081-230-3 < 1 -- < 1 -- < 1 -- < 1 -- < 10

PB-0213086 Procedural blank2 < 1 -- < 1 -- < 1 -- < 1 -- 5

MB-021408 Matrix blank < 1 -- < 1 -- < 1 -- < 1 -- < 10

MS-PCB/PBDE-021308 Matrix spike - PCBs/PBDEs 19 11 18 11 16 9 < 1 -- 170

PC-021408 Positive control Saginaw 

carp 6806

< 1 -- < 1 -- < 1 -- < 1 -- < 10

Baseline PC data: Positive control Saginaw 

carp 6806

-- -- -- -- -- -- -- -- 10

1

 PBDE-183 not recovered, degradation via gas chromatography column activity.

2

 Procedural blank values are reported as total amounts (not concentrations), are adjusted for surrogate recovery, and are not corrected for background values.
Tables 1–4 21

Publishing support provided by: 

Rolla Publishing Service Center 

For more information concerning this publication, contact:

Director, USGS Columbia Environmental Research Center

4200 New Haven Road

Columbia, MO 65201

(573) 875–5399

Or visit the Columbia Environmental Research Center Web site at:

http://www.cerc.usgs.gov
Gale and others—Determination of Biphenyls, Pesticides, and Flame Retardants in Fillets of Fishes from MDC Monitoring Programs–2007—Open-File Report 2009–1053