TIE ACUTE TOXICITY OF HBCD LOT 990.;;:17 TO Tl£ BL1JEGILL SUNFISH L£POMIS MACROCHIRUG RAFINESQUE '-!lTH TEST DATA AND 

### COVER ·LETTER

### March**~. l990~** 



### Document Processing Center (TS-790) RoomL-lOO,.Office of Toxic Substances Environmental Protection Agency 401 

### M street, sw Washington, DC 2046.0 

### Attn: B(d} Health & 

### Safety Reporting ( Noti fica tionjReport.i ng) Dear Sir:
In response t6 the TSCA Section 8 (d) 

### rule p\.lbllshed in the December 12, 1989 

### Federal Register, the enclosed information is**beingsub~** 

### mitted on the following substances: ·**2,4,6-~ribtomophenol** 

### Tetrabromophthalic anhydride Hexabromocyclododecane**1,2-Bis(~rib':"omophenoxy)ethane** 

### Decabrornodiphenyl ether Pentabromodiphenyl ether Octabromodiphenyl ether CAS Number
118-79-6 632-79-1**3194~55-6** 37853-59-1 1:;.63-19-5

.**3~534-81-9** 32536-52-0 

### The studies on the first four compounds are submitted on beha.lf of ·the following manufacturing**faci~ity:** 

### Great Lakes.Chemical corporation Route 7, Box 529 · ELDorado, AR 71730 

### studies onthe last three compounds following manufacturing facility:

Great Lakes Chemical Co't"poration Highway 15 south, 

## P.o. Box 1878. · El Dorado, AR**71731~18.18**- '-- C"

- -- - --

c 

### There are
. no

### know~)" .additives to these. COlnpoumis

### ~ .·· Pentabrofuodiphenyl _ether consists of hexa...;,.**p~nta-,** 

### tetra-bromodiphenyl eth.er. Octabromcidiphenyl ether consists of nona-,**octa-~·** 

### hepta,-. 1 ._and,hexabromodiphenyl ether. We· are not aware of l;.ny ongoing stud.j.es with·.·

·· any.of the listed compounds. If you have any**q~estio:ns** 

### concerning _this sublllission, please call me at (317) 497-6391.



### · Sincerely, 

# :D.-e1 tk 

## d/? · /_/c

# /~2/ <-A_ 

### Dennis 

### L• ·McFadden, Ph.D. ProductSafety Coordinator

DLM:sb:030890 Enclosures

### Acute toxicity_studies in ratsand rabbits Primacy skin in:itation study in albino rabbits Eye irritation study in albinorabbits

_ · Acute inhalation toxicity in the albino rat Acute dermal toxicityin Dlaleand-fellale albino rabbits Acute opal toxicity (LD 50 ) 

### study in albino rats Dermal sensitization study .inthe albino guinea pig

_ _ _ Bioaccumulation >study in fat tissue with 2, 4, 6-tribromophenol

. 48-Hour dynamic aquatic toxicity_ study with 2 1 

### 4, 6-tribromophenol in Daphnia · _ ·_ · _ Four-day static aquatic toxicity studies with 2,4,6-tribromo..;.

· phenol in rainbow trout and bluegills Screening testfor microbiological inhibition with2,4,6- tribromophenol

_ _ _ - Acute dust inhalation toxicity study with 2, 4, (>.;..tribromophehol, lot number 3106 ih rats 28-Day subacute dermal toxicity study with 2,4,6-tribromophenol in albino rabbits (10/27/77) 28-Day subacute dermal toxicity study wth 2,4,6-tribromophenol in albino rabbits ( 12/12/77) · · · Pilot teratology study in rata Mutagenicity evaluation of 2,4,6-tribromophenol, lot number .J287, in_the Ames Salmonellil/Microsome Plate Test Acute oral t.oxicity. (LD 5 

## oJ st1..•dy in rats

__ _ The bioaccurnulation of 2,4,6-tribromophenol in bluegillsunfish2, 4, 6-TribromC>phEmol biodegradation (9/30/75) -2,4,6-Tribrornophenoi (TBP) biodegradation (6/1/76)

Pharmacokinetics study of 2,4,6.;.tribrornophenol in rats Photolysis of**2,4,6-t~ibromophenol** 

### _ · · Partition coefficient of several flame**retardants~and indust~ial**  

### chemicals Water**solubilit~** 

### of several flame retardants and industrial chemicals ·_Photolysis of 2,4,6-tribrornophenol in aqueous solution

### Acute.oralstudy

·. Acute dermal study

. .· ...

. Acute toxicitystudies of tet:rabromophthalic.anhydrfde

Primacy s1dn ··irritation study in albino rabbits Eye irritation_study.in albino rabbits Acute inhalation toxicity in the albino rat Dermal sensitization study in the albino guinea pig Acute inhalat1on toxicity in the albino rat after (3/12/75)

. 

### Acute inh<llation toxicity in the albino rat. 

# after pyrolysis (3/26/75) 

### 28-bay dennal toxicity study in rabbits 2l;...Day inhalation toxicity in rats Human repeated insult patch test with Firemaster PHT4 ,;tutagenicity evaluation of 859-74-4 Pilot:. teratology study in rats The. '-'CUte toxicity of Firemaster PHT4 to the water flea, Daphnia magna Straus The acute toxicity of Firemaster PHT4 I 

### lot humber 63:32-B to the rainbow trout Salmo gairQ.ngtl Richardson The acute toxicity of Firemaster PHT4, lot number 6332-'B 

### to the bluegill sunfish tepomis macrohfrus.Rafinesque Acute oral toxicity study in mice · · Comparative inhalation toxicity tests Evaluation of the inhalation toxicity ofcombustion products of selected polymers II· The bloaccumulation of tetrabromophthalicanhydride in the bluegill sunfish Persisterice of**PHT~4** 

### in soil at 1**~nd** 

### 10 

### pgjg Photolysis of PHT-4 Effec:its.of PHT-4 on soil microflora at 1 

### and 

### 10 J,lgjg Pharmacokinetics of r>HT-4 ·in rats

. Partition coefficient of several flame retardants and industrial chenicals

. Water solubility of several flame retardants and industrial·. chemicals

### /.Hexabromocyclododecane: 28-clay' feeding trials**~** 

### ... · 90-:-day feeding trials wi thrats vrnvitro microbiological mutgenicity Geigy Corporation compOunds Pilot cataractogenic study in chicks Cataractogenic study in- chicks Mutagenicity evaluation of 421-32B Acute toxicity studies in rabbits ad rats

. Mutagenicity of**~two** 

### lots of FM-100, lot 53 and residue of.

· lot 3322 

### in the absence and presence- of metabolic

activation

.. . · The acute toxj,ci t.y of .HBCD lot numbez- 990-17 · t.o the bluegi].l

. . sunfish Lepomis macrohirus Rafinesque

. · ·

. Partition coefficient of dicamba, eridrin, VEL 3510 and several industrial chemicals and flame retardants Water solubility of several industrial chemicals I 

### flame

· :retardants and a herbicide VEL 3510**H~drol~sis** 

### of Firemaster 100 · HBCD

- biodegradability study

### -Water solubility of sevetal flame retardants and**industrial·che~icals** 

### ._photolysis of Firemaster 680 -Pharmcokinetic study of FM-680 -Rate of hydrolysis studies .;..partition coefficient of MC-680, ·chlorendic anhydride and chlorendic acid -28-Day rat feeding study --Acute oral toxicity study in the albino rat.· -Acute dermal. LD 50 ( 

### 1 level) -Acute inhalation t

....**~·icity** 

### in the albino rat -Acute oral toxicity study in beagle dogs -Acute toxicity**(T~ )**  

### study in the bluegill ...;Acute toxicity (LD 50 ) 

### t;;tudy in the rainbow trout -Acute heated vapor inhalation toxicity study in rats .;..Modified Draize multiple insult test in male humans ....--28-bay dermal toxicity study in rabbits**~21-Day** 

### inhalation toxicity in rats -Acute inhalation toxicity in the albino rat after

· pyrolysis (ABS resin/F!tt-680/Sb 0 3 ) 

### Acute inhalation toxicity in the albino rat after pyrolysis (ABS resin/Sb 2 

### 0 3 ) 

### --14-Day range fnding study in rats '28-Day toxicity study in t'ats '-Biodegradation study with He-tagged MC-680 -Biodegradability study with 14 

### c-tagged MC...;680 "Primary skin irritation test with mother liquor 680 process, code no. 7-29-76-B in albino rabbits**~skin** 

### sensitization**t~st** 

### with mother liquor 680 process iri albino gi1inea pigs --90-Day subacute oral toxicity study with Firemaster 6BO



### , in albino rats (Jan. 6, 1977) '-90-Day subacute oral toxicity study with Firemaster 680 in albino rats (Mari 10, 1977) 'Mutagenicity evaluation of 859-74-5 final :t"eport -Test report (method for testing the degree of accumulation of chemical substances in fish body) (Bioaccumulation in fish) 'Acute inhalationtoxicity.study in thealblno rat after pyrolysis (ABS**resinjSb~Oycontaining** 

### 680) Pilot teratology study in rats 'Teratology study in rats '-'!'issue residue accumulation/depletion study with Y.'in•master 680 in albino rats---: _- - - . __ '

_-**~-** -- - - --



### . \Ac\lte tcxicity studies· in rat!;; alid rabbits

\Mutagenh':ity evalu.r1tion 

## of compound 364..;5A '-4!8-Day to.'<icity study in r,ats (6/12/74) '28..;Day toxicity study in rats (6/24/76) '213-Day toxicity study in rats (J/2/77)

. 'Mutagenic_:ity evaluation of compound 277-10

### ~utagenicity evaluation of compound 345-76A final rep<:>rt 'The acute oral toxicity of pentabromodiphenyl ether to rats '\Acute toxicity studies in rats and rabbits \28-Day toxicity study in rats "rhe bioaccumuJ.atiori of compound S-512 by carp**~~o.30-Daydietarystudy** 

### in rats withpentabromodiphenyl oxide

. · . including recovery periods of 6, 12, and 24 weeks ,90-Day die·tary study in rats with pentabromodiphenyl

· oxide (DE-71) · Octabromodiphenyl oxide 

# J The bioaccumulation of compound S-511 by carp

...-Acute toxicity studies in.· rats and rabbits v1subacute inhalation toxicity study in rats

. ·. JMutagenicity evaluation of compound 345-79A final report Jinvitro sister chromatid exchange in Chinese hamster ovary cells with oct.abromodiphenyl ether (DE-79) J,unscheduled DNA synthesis assay, compound DE-79 "28-Day toxicity study in rats '13-Week feeding study in rats 'A range-finding teratology study in**rat~** 

### with DE-79THE ACUTE TOXICITY OF
HBCD

### ;./e;e~tbNI<t~c 

## yc (o dod ect(ue LOT #990-17 · 

### CA.N/. ,3/9{- 

### :_SS-t
' TO THE
BLUEGILL SUNFISH Leoomismacrochirus Rafinesque

Prepared For
Velsicol Chemical Corporatibn Chicagoj Illinois

Prepared By
UNION CARBIDE ENVIRONHENTAL SERVICES Union Carbide Corporation

. Tarrytmm, New York 10591

October 

### :3, 1978

# . J -**~** . - --- - - ENVI RONM£NTAl SER\liCES

· 1 M"HlYTOWN HCHtm:;,\L CENTER

. 1Ahnv·TO\;J'i

. .-.;rw vom: 10591 • f•q'

Date:

UCES Proj. Nb. :
summary:

Species: Length:**~\eight:** Source:
96 

### Hour LCs o: 95% Conf. Limits:
96 

### Hour Observed No Effect**Level~** Nater Quality: Temperature: pH:
Total Hardness
as CaC03:
96 Hr. LCso 

### Ref.**~o~icant** p,p'-DDT:
Notebook Ref.: Velsicol Che:nica:l**Cc-rporat~.on.** The test was**conduct~d** 

### from**Sept~mber** through September 

### 11, 1978. 

### i:ECD Lot i!':;l90-l7
ll506-03-77
The96 hour LCso for HBCD (Lot 5990-17) 

### to bluegill**sunfi~h** is greatex than 100.0 mg/l. This**valu~** is 

### based tioon nominal**concentra~** tions 6f the chemical.in soft recdristitutbd \:;a ter.

Bluegill SUCI1Sn
44 

### nun 1.38 gr.:1ms
Nebraska
>.lOO.O mg/1
Not available

100.0 mg/1 

### Soft

7.50

. 42 r.lg/1

4 • 0 3 ( 3 

## .s 9 - 4 • 52)**~** 

### g/ 1

# I 

# I I - -_-

-- -**~-** --**-·~:ai conduct~(l**  

### i!t. the**•requ~st**

- -**~** ' - - --

- -

-

__**c..,,<~•_.,o·-·a 1**  r---,.,....;,...
....**,f--~',.._:n-** ·h·>**-(~At-r>r..-.;·0·,._.** "'},_,;-S;c..,t--1· 

### c- -,_ .**..,""~···-L~-"'_,A.. "'·"L,--..._::..-"'...:~(._,._._~t..,..~•-**  """-'-

..... .,.**~"""'~_'P"_-** "'::"" ll..

.... .-**""""~--**
:!**~90-17,** to the bluegill sunfish.

- - - fo::-med at Union Carbide Environmental**Set~vices' laborato~y**  in**Tarrytown~ Ne~** **Yo~k.** 

### is moderately soluble in acetone. I!BCD formed 

### a fine precipitate in the stock solution which Has suspended 

## by agitation for dispensing into the te!>;:. vessels.- A white flocculate was**fo~med** on-the surface of the water in 

### all test**~on** centrations upon addition of the stock.
The bluegill sunfish, Lenomis macrochirus Rafinesque 1 is



### a \.;arm-\vater fish usually found in ponds,**lal~es** and sluggish

streams**~ith** bottmns of sandi**g~ave~** or mud. They feed on a variety of aquatic organisms including fish eggs, small fish, snails, insects andamphipods. Bluegills prefer temperatures
above 20°C and can tolerate a Hide pH range. Because of their
wide geographic distz.·ibution, temperature requirel"lents, and 

## j- importance as a food \veb organism, the bluegill sunfish has
been recommendedby the Committee on Nethods for Toxicity 

# I J \.'ith Aquatic Organisms (1975) as a bioassay orgLnism.
-NETHODS

Dilution Hater used in all basic toxicity
UCES laboratory is obtained from a \veil on the
treated vlib1 a Continental Heverse Osmosis

### I


# .. I· - --=--- --- - - ·• trea 

### t!ilent, : the

. vltl 

## te i

. is

to the desired pH

### a~d h;-u;dne':ls according. 

### to 

## the

of 1·1arking and Dilh'SOn ( 1973}. For this test the 

## sofe
tuted water Has characteri?.,ed 

## as havin';} a 

### pH of 

### 7 • SO; 

### total**h~idnes~o£** 42 

### mg/1**~s caco~,**  total**~lkalinity ~f**  

### 31 mg/1 as

· CaCO J 1 and a sped fie conductance of. 160 Iimhos;cm. · llardhess
and alkalinity were determined**accordin~** to**standat~d** analytical procedures (Amedcan Publh-:. Health Association, 1976} 

### i pB with

an ORION pH Heter I conductivity with a XSI Conductivity
and dissolved oxygen with a YSI Oxygen Het.er.
Five concentrations, a control and solvent control were

gill sunfish. Test rnethodology follo-..:ed recorr
... nended bioassay practices (U.s. Enviromnental Protection l1gency, 1975} with the

excr 1tion that replicate conccr"ltrations \·.et"e not: used. Fresh
stock solution for the test 

### Has**pz~pared** by weight to a precision of 0.1 mg and diluted to volume iri volumett-ic ulassware
with reagent grade acetone. The test 

### ,,,a.:;; conducted in 19.6 liter, chemidelly clean glass jars containing 

### 15 liters of

water. 'rhe test was started by introducing the toxicant into

test vessels**co:~taining** dilution \vater, thoroughly mixing, and
then intrcaucing the fish, 'l'he amount of solvent in the solvent
control**equa~** l0d ·that. amount used in "the highes.t. concentration. Bluegill sunfish t·Jere obtained from a conunercial hatchery
in Nebraska and were maintained in the .UCES la'..)orator:y at. 22°C**acco.t~dinl]** oto 

### tfl_e 0 procedures
:·!ort<: lities in the stock c:'ul ture 

### over a one ;:erio-:1 ',·:ere less than bib percent. Blu8gil1 sunfish at
:Cir .• cof testing were upproximatcly· 4 months old and had (lO organisms) length of 44 mm and a mean weight of Fi::;h used in this test were randomly selected from the stock
cul tnre and acclirr.ated to the test water for 24 hot!rs prior to testing. Forty-eight hours before initiating the tP.st the
fish were taken off feed. Ten**indiv~du~ls** were placed in each
of the 19; 6 liter test vessels. Biological loading was 0:.92 gjl.

Dissolvc:d oxygen and pt-1 were determined ini t.ially and every 

### 48 hours thereafter for the control, solvent control, high;
medium and lev.; toxicc.nt concentrations.**~'later** bath temperature

was deter.:nined initially and at 4 8 hour intervals subsequent

to the initiation of the**~est.** In additio!l to obtaining tht:!
above chemical and physical parameters, abnormal behnvi6.ra1

responses of the test fish \vere noted and reco.rded at 24 hour intervals.
The concentration of toxicant lethal to SO% of the population (LC 50 ) and 95t, confidence limits could not be determined**d~e** to the absehce 6f mortalities. Test results were**b~sed** uport nomj :1al concentrations of the t.est material in**sof~** reconstituted water. -The nb effect level was determined At the 

### 96**hourex~osure** pericid. This

### v~lue is GasGd upon the absence of96 hour LC 50 for HBCD, Lot #990-17, to bluegill sun

.... ·fish is >100.0 mg/1. Test results are presented in Table 1.
The chemical and physic3.l parameters monitored during the

are**pr.~sented** in Table 2. Behavioral observations made during
the test indicated no observable abnormal beha•Jior in any concentration throughout the test •.
It should be noted that LCs o values may vary with different species, temperatures and \vater qualities.---'..Table1PercentI·lo.ctalitiesandLCs0ValuesClient:Vclsico1ChemicalCoq:Joration.Test!·lateria.l:.II!3CD;.Lot11990-17TestOrganism:DluegillSunfishr:-"'"----,-'--_:__--,-,_:...__.c:..t!-lor...:...i..:...t;:..y..:-,___··:···-·-··:···:·:--::--..-····.-··········Test!·latcrial,Nominal··conc;.rnq/J·controlIr;oI.32.o--·----·-··I::f:;''-----'--LCnValues48·nour96HourLCso·mg;l>100.0>100.0>100.0Confidence··....·.·.·.:95'Lo·•N.•A.IN.A.±it=N.A..·.··..··..Limi,tSHigh.··N.l\o,N.A.,,!LA.=NotavaiIableduetoabsenceofmortalities.The9 6hourobservednoeffectlevelis100.0P.lg/LUNIQf.JEtlV!RONt·1BNTAL·SEnVICESI,'"''',--Initial4.8hour96hour:._.J·-·-·-·;)2 -Physicalanc1ChemicalParametersClient:TestTestSpecificConcJI.lcti1nce·VelsicoiChcmical.CorporatioriIli3CD,Lot#990-17.BluegillSunfishDilutionWater_.1Averagencllanlnessmg/1u:>_CuCO3TotalAlk<:Jlinityrnq/1as.CaCOJ.Control8.57.34•.4IControl7.506..907.0716020.3±0.74231Oxygen,mg/1SolventConl:roJ.8.57..22.5SolventControl7.496.886.90TestN<:lterial,Nomi!'lalCone..mg/1-------r10.0328.6.8.56.87.0--2.2I..1.9p!ITes.tMateriaf,NomirialCone.mg/110.0;2•0100•07.497..4 96.87..6.896•.85··.··j....JUNIONAl-Tierica.n Public Health Association; 1976. Standard 1>1ethods _£or the Examination of \vater and h'astewater, 14th ed. New**York~** 1193 

### pp. Brauhn, 

### J. L., R. A. Schoettger and L. H.

### Nueller~ 1975. Acquisition and Culture of Research Fish: Rainbow Trout, Fathead Mirinows, Channel Catfish and-Dluegills.; EPA-660/3-75-001. 45 

### pp.

- Commjttee on Nethods for Toxicity Tests with Aquatic Organisms.

-- 1975. Nethods for Acute Toxicity 'l'ests withFish, Nacreinvertebrates and Amphibians. EPA-660/3-75-009. 61 PP• !-1arking, L. L. and V. K. Dawson. 1973. Toxicity of Quinaldine Sulfate to Fish. Invest. Fish Control No. 48. 

## u.s. Fish

- Wildl;

### ~erv.,**Washington~** D.C. 8 

### PP•\'l. Calmbacher Aquatic Toxicologist

RcportApproved By

JUgJ.iLdas G. Vilkas Section Leader

. Aquatic Toxicology

Report Approved By· 

# ( ) t

.. ·. 

# 1\·+f;

I..

- 'I

- - -

### ~-'l.,,): <(). \ _t,.- \ .-J. ,( .1-. ,, Curt Hutchinson Hanager UNION CARBIDE**CORPOfu~TION** ENVIRONt,lENTAL SERVICES

October 

### 3, 197 8

### .· CERTIFICATf·OF AUTHENTICITY

TriiS IS TO CERTIFY that the microimages appearing on this microfiche are accurate

- - - - and complete reproductions of the records of U.S; Environmental Protection Agency**documa~ta** as delivered_ in the regular course of business for microfilming.

Data produced 

# f · ·**~3** (Month) (Day)

New York (State) 

# 9o (Year)

# 0~~ Camera C?perator 

# a
AMTEK.

·corp