TIE ACUTE TOXICITY OF HBCD LOT 990.;;:17 TO Tl£ BL1JEGILL 

SUNFISH L£POMIS MACROCHIRUG RAFINESQUE '-!lTH TEST DATA AND 

COVER ·LETTER 
March ~. l990~ 

Document Processing Center (TS-790) 

RoomL-lOO,.Office of Toxic Substances 

Environmental Protection Agency 

401 M street, sw 

Washington, DC 2046.0 

Attn: B(d} Health & Safety Reporting 

( Noti fica tionjReport.i ng) 

Dear Sir: 

In response t6 the TSCA Section 8 (d) rule p\.lbllshed in the December 

12, 1989 Federal Register, the enclosed information is beingsub~ 

mitted on the following substances: · 

2,4,6-~ribtomophenol 

Tetrabromophthalic anhydride 

Hexabromocyclododecane 

1,2-Bis(~rib':"omophenoxy)ethane 

Decabrornodiphenyl ether 

Pentabromodiphenyl ether 

Octabromodiphenyl ether 

CAS Number 

118-79-6 

632-79-1 

3194~55-6 

37853-59-1 

1:;.63-19-5 . 

3~534-81-9 

32536-52-0 

The studies on the first four compounds are submitted on beha.lf of 

·the following manufacturing faci~ity: 

Great Lakes.Chemical corporation Route 7, Box 529 · ELDorado, AR 71730 

studies onthe last three compounds 

following manufacturing facility: 

Great Lakes Chemical Co't"poration 

Highway 15 south, P.o. Box 1878. · 

El Dorado, AR 71731~18.18 
- '-- C" - -- - --

c There are . no know~)" .additives to these. COlnpoumis ~ .·· Pentabrofuodiphenyl _ether consists of hexa...;,. p~nta-, tetra-bromodiphenyl eth.er. 

Octabromcidiphenyl ether consists of nona-, octa-~· hepta,-. 1._and,hexabromodiphenyl ether. We· are not aware of l;.ny ongoing stud.j.es with·.· ·· any.of the listed compounds. If you have any q~estio:ns concerning 

_this sublllission, please call me at (317) 497-6391. 

· Sincerely, 

:D.-e1 tk d/? · /_/c /~2/ <-A_ 

Dennis L• ·McFadden, Ph.D. 

ProductSafety Coordinator 

DLM:sb:030890 

Enclosures 
Acute toxicity_studies in ratsand rabbits 

Primacy skin in:itation study in albino rabbits 

Eye irritation study in albinorabbits _ · Acute inhalation toxicity in the albino rat 

Acute dermal toxicityin Dlaleand-fellale albino rabbits 

Acute opal toxicity (LD50) study in albino rats 

Dermal sensitization study .inthe albino guinea pig _ _ _ Bioaccumulation >study in fat tissue with 2, 4, 6-tribromophenol . 48-Hour dynamic aquatic toxicity_ study with 2 1 4, 6-tribromophenol in Daphnia · _ ·_ · _ 

Four-day static aquatic toxicity studies with 2,4,6-tribromo..;. · phenol in rainbow trout and bluegills 

Screening testfor microbiological inhibition with2,4,6-

tribromophenol _ _ _ - Acute dust inhalation toxicity study with 2, 4, (>.;..tribromophehol, lot number 3106 ih rats 

28-Day subacute dermal toxicity study with 2,4,6-tribromophenol in albino rabbits (10/27/77) 

28-Day subacute dermal toxicity study wth 2,4,6-tribromophenol in albino rabbits ( 12/12/77) · · · Pilot teratology study in rata 

Mutagenicity evaluation of 2,4,6-tribromophenol, lot number 

.J287, in_the Ames Salmonellil/Microsome Plate Test 

Acute oral t.oxicity. (LD5oJ st1..•dy in rats __ _ The bioaccurnulation of 2,4,6-tribromophenol in bluegill sunfish2, 4, 6-TribromC>phEmol biodegradation (9/30/75) 

-2,4,6-Tribrornophenoi (TBP) biodegradation (6/1/76) 

Pharmacokinetics study of 2,4,6.;.tribrornophenol in rats 

Photolysis of 2,4,6-t~ibromophenol _ · · Partition coefficient of several flame retardants~and 

indust~ial chemicals 

Water solubilit~ of several flame retardants and industrial 

chemicals 

·_Photolysis of 2,4,6-tribrornophenol in aqueous solution 
Acute.oralstudy 

·. Acute dermal study . .· ... . Acute toxicitystudies of tet:rabromophthalic.anhydrfde 

Primacy s1dn ··irritation study in albino rabbits 

Eye irritation_study.in albino rabbits 

Acute inhalation toxicity in the albino rat 

Dermal sensitization study in the albino guinea pig 

Acute inhalat1on toxicity in the albino rat after 

(3/12/75) . 

Acute inh<llation toxicity in the albino rat. after pyrolysis 

(3/26/75) 

28-bay dennal toxicity study in rabbits 

2l;...Day inhalation toxicity in rats 

Human repeated insult patch test with Firemaster PHT4 

,;tutagenicity evaluation of 859-74-4 

Pilot:. teratology study in rats 

The. '-'CUte toxicity of Firemaster PHT4 to the water flea, 

Daphnia magna Straus 

The acute toxicity of Firemaster PHT4 I lot humber 63:32-B 

to the rainbow trout Salmo gairQ.ngtl Richardson 

The acute toxicity of Firemaster PHT4, lot number 6332-'B 

to the bluegill sunfish tepomis macrohfrus.Rafinesque Acute oral toxicity study in mice · · Comparative inhalation toxicity tests 

Evaluation of the inhalation toxicity ofcombustion products of selected polymers II· 

The bloaccumulation of tetrabromophthalicanhydride in the 

bluegill sunfish 

Persisterice of PHT~4 in soil at 1 ~nd 10 pgjg 

Photolysis of PHT-4 

Effec:its.of PHT-4 on soil microflora at 1 and 10 J,lgjg Pharmacokinetics of r>HT-4 ·in rats . Partition coefficient of several flame retardants and 

industrial chenicals . Water solubility of several flame retardants and industrial·. 

chemicals 
/.Hexabromocyclododecane: 28-clay' feeding trials ~ ... · 90-:-day feeding trials wi thrats 

vrnvitro microbiological mutgenicity 

Geigy Corporation compOunds 

Pilot cataractogenic study in chicks 

Cataractogenic study in- chicks 

Mutagenicity evaluation of 421-32B 

Acute toxicity studies in rabbits ad rats . 

Mutagenicity of ~two lots of FM-100, lot 53 and residue of. · lot 3322 in the absence and presence- of metabolic 

activation .. . · The acute toxj,ci t.y of .HBCD lot numbez- 990-17 · t.o the bluegi].l . . sunfish Lepomis macrohirus Rafinesque . · · 

. Partition coefficient of dicamba, eridrin, VEL 3510 and 

several industrial chemicals and flame retardants 

Water solubility of several industrial chemicals I flame · :retardants and a herbicide VEL 3510 H~drol~sis of Firemaster 100 · HBCD - biodegradability study 
-Water solubility of sevetal flame retardants and industrial·che~icals 

._photolysis of Firemaster 680 

-Pharmcokinetic study of FM-680 

-Rate of hydrolysis studies 

.;..partition coefficient of MC-680, ·chlorendic anhydride 

and chlorendic acid 

-28-Day rat feeding study --Acute oral toxicity study in the albino rat.· 

-Acute dermal. LD50 ( 1 level) 

-Acute inhalation t .... ~·icity in the albino rat 

-Acute oral toxicity study in beagle dogs 

-Acute toxicity (T~ ) study in the bluegill 

...;Acute toxicity (LD50 ) t;;tudy in the rainbow trout 

-Acute heated vapor inhalation toxicity study in rats 

.;..Modified Draize multiple insult test in male humans 

....--28-bay dermal toxicity study in rabbits ~21-Day inhalation toxicity in rats 

-Acute inhalation toxicity in the albino rat after · pyrolysis (ABS resin/F!tt-680/Sb 03 ) 

Acute inhalation toxicity in the albino rat after 

pyrolysis (ABS resin/Sb203) 

--14-Day range fnding study in rats 

'28-Day toxicity study in t'ats 

'-Biodegradation study with He-tagged MC-680 

-Biodegradability study with 14c-tagged MC...;680 

"Primary skin irritation test with mother liquor 680 

process, code no. 7-29-76-B in albino rabbits ~skin sensitization t~st with mother liquor 680 process 

iri albino gi1inea pigs --90-Day subacute oral toxicity study with Firemaster 6BO 

, in albino rats (Jan. 6, 1977) 

'-90-Day subacute oral toxicity study with Firemaster 680 

in albino rats (Mari 10, 1977) 

'Mutagenicity evaluation of 859-74-5 final :t"eport 

-Test report (method for testing the degree of accumulation 

of chemical substances in fish body) (Bioaccumulation 

in fish) 'Acute inhalationtoxicity.study in thealblno rat after 

pyrolysis (ABS resinjSb~Oycontaining 680) 

Pilot teratology study in rats 

'Teratology study in rats 

'-'!'issue residue accumulation/depletion study with Y.'in•master 

680 in albino rats 
---: _- - - . __ ' _- ~- -- - - --

. \Ac\lte tcxicity studies· in rat!;; alid rabbits 

\Mutagenh':ity evalu.r1tion of compound 364..;5A 

'-4!8-Day to.'<icity study in r,ats (6/12/74) 

'28..;Day toxicity study in rats (6/24/76) 

'213-Day toxicity study in rats (J/2/77) . 'Mutagenic_:ity evaluation of compound 277-10 

~utagenicity evaluation of compound 345-76A final rep<:>rt 'The acute oral toxicity of pentabromodiphenyl ether to rats 

'\Acute toxicity studies in rats and rabbits 

\28-Day toxicity study in rats 

"rhe bioaccumuJ.atiori of compound S-512 by carp ~~o.30-Daydietarystudy in rats withpentabromodiphenyl oxide . · . including recovery periods of 6, 12, and 24 weeks 

,90-Day die·tary study in rats with pentabromodiphenyl · oxide (DE-71) · 

Octabromodiphenyl oxide 

J The bioaccumulation of compound S-511 by carp 

...-Acute toxicity studies in.· rats and rabbits 

v1subacute inhalation toxicity study in rats . ·. JMutagenicity evaluation of compound 345-79A final report Jinvitro sister chromatid exchange in Chinese hamster ovary 

cells with oct.abromodiphenyl ether (DE-79) 

J,unscheduled DNA synthesis assay, compound DE-79 

"28-Day toxicity study in rats 

'13-Week feeding study in rats 

'A range-finding teratology study in rat~ with DE-79 
THE ACUTE TOXICITY OF 

HBCD ;./e;e~tbNI<t~c yc (o dod ect(ue 

LOT #990-17 · CA.N/. ,3/9{- :_SS-t 

' TO THE 

BLUEGILL SUNFISH 

Leoomismacrochirus Rafinesque 

Prepared For 

Velsicol Chemical Corporatibn 

Chicagoj Illinois 

Prepared By 

UNION CARBIDE ENVIRONHENTAL SERVICES 

Union Carbide Corporation 

. Tarrytmm, New York 10591 

October :3, 1978 
. J 

- ~ . - --- - -

ENVI RONM£NTAl SER\liCES 

· 1 M"HlYTOWN HCHtm:;,\L CENTER 

. 1Ahnv·TO\;J'i . .-.;rw vom: 10591 • f•q' 

Date: 

UCES Proj. Nb. : 

summary: 

Species: 

Length: 

~\eight: 

Source: 

96 Hour LCs o: 

95% Conf. Limits: 

96 Hour Observed 

No Effect Level~ 

Nater Quality: 

Temperature: 

pH: 

Total Hardness 

as CaC03: 

96 Hr. LCso Ref. ~o~icant p,p'-DDT: 

Notebook Ref.: 

Velsicol Che:nica:l Cc-rporat~.on. 

The test was conduct~d from Sept~mber 

through September 11, 1978. 

i:ECD Lot i!':;l90-l7 

ll506-03-77 

The96 hour LCso for HBCD (Lot 5990-17) to 

bluegill sunfi~h is greatex than 100.0 mg/l. 

This valu~ is based tioon nominal concentra~ 

tions 6f the chemical.in soft recdristitutbd 

\:;a ter. 

Bluegill SUCI1Sn 

44 nun 

1.38 gr.:1ms 

Nebraska 

>.lOO.O mg/1 

Not available 

100.0 mg/1 

Soft 

7.50 

. 42 r.lg/1 

4 • 0 3 ( 3 .s 9 - 4 • 52) ~ g/ 1 
I 

I 

I 

- -_- -- - ~- --

-·~:ai conduct~(l i!t. the •requ~st - - ~ ' - - --

- - -

__ c..,,<~•_.,o·-·a 1 r---,.,....;,... .... ,f--~',.._:n- ·h·> -(~At-r>r..-.;·0·,._. "'},_,;-S;c..,t--1· c- -,_ . ..,""~···-L~-"'_,A.. "'·"L,--..._::..-"'...:~(._,._._~t..,..~•- """-'- ..... .,. ~"""'~_'P"_- "'::"" ll.. .... .- """"~--

:! ~90-17, to the bluegill sunfish. 

- - -

fo::-med at Union Carbide Environmental Set~vices' 

laborato~y in Tarrytown~ Ne~ Yo~k. 

is moderately soluble in acetone. I!BCD formed a fine 

precipitate in the stock solution which Has suspended by agitation for dispensing into the te!>;:. vessels.- A white flocculate was fo~med on-the surface of the water in all test ~oncentrations upon addition of the stock. 

The bluegill sunfish, Lenomis macrochirus Rafinesque 1 is 

a \.;arm-\vater fish usually found in ponds, lal~es and sluggish 

streams ~ith bottmns of sandi g~ave~ or mud. They feed on a 

variety of aquatic organisms including fish eggs, small fish, 

snails, insects andamphipods. Bluegills prefer temperatures 

above 20°C and can tolerate a Hide pH range. Because of their 

wide geographic distz.·ibution, temperature requirel"lents, and 

j- importance as a food \veb organism, the bluegill sunfish has 

been recommendedby the Committee on Nethods for Toxicity 

I 

J 

\.'ith Aquatic Organisms (1975) as a bioassay orgLnism. 

-NETHODS 

Dilution Hater used in all basic toxicity 

UCES laboratory is obtained from a \veil on the 

treated vlib1 a Continental Heverse Osmosis 
I 

.. 

I· 

- --=--- --- - -

·• trea t!ilent, : the . vltl te i . is 

to the desired pH a~d h;-u;dne':ls according. to the 

of 1·1arking and Dilh'SOn ( 1973}. For this test the sofe 

tuted water Has characteri?.,ed as havin';} a pH of 7 • SO; total 

h~idnes~o£ 42 mg/1 ~s caco~, total ~lkalinity ~f 31 mg/1 as 

· CaCO J 1 and a sped fie conductance of. 160 Iimhos;cm. · llardhess 

and alkalinity were determined accordin~ to standat~d analytical 

procedures (Amedcan Publh-:. Health Association, 1976} i pB with 

an ORION pH Heter I conductivity with a XSI Conductivity 

and dissolved oxygen with a YSI Oxygen Het.er. 

Five concentrations, a control and solvent control were 

gill sunfish. Test rnethodology follo-..:ed recorr ... nended bioassay 

practices (U.s. Enviromnental Protection l1gency, 1975} with the 

excr 1tion that replicate conccr"ltrations \·.et"e not: used. Fresh 

stock solution for the test Has pz~pared by weight to a precision of 0.1 mg and diluted to volume iri volumett-ic ulassware 

with reagent grade acetone. The test ,,,a.:;; conducted in 19.6 

liter, chemidelly clean glass jars containing 15 liters of 

water. 'rhe test was started by introducing the toxicant into 

test vessels co:~taining dilution \vater, thoroughly mixing, and 

then intrcaucing the fish, 'l'he amount of solvent in the solvent 

control equa~ l0d ·that. amount used in "the highes.t. concentration. 

Bluegill sunfish t·Jere obtained from a conunercial hatchery 

in Nebraska and were maintained in the .UCES la'..)orator:y at. 22°C 
acco.t~dinl] oto tfl_e 0 procedures 

:·!ort<: lities in the stock c:'ul ture over a one 

;:erio-:1 ',·:ere less than bib percent. Blu8gil1 sunfish at 

:Cir .• cof testing were upproximatcly· 4 months old and had 

(lO organisms) length of 44 mm and a mean weight of 

Fi::;h used in this test were randomly selected from the stock 

cul tnre and acclirr.ated to the test water for 24 hot!rs prior to 

testing. Forty-eight hours before initiating the tP.st the 

fish were taken off feed. Ten indiv~du~ls were placed in each 

of the 19; 6 liter test vessels. Biological loading was 0:.92 

gjl. 

Dissolvc:d oxygen and pt-1 were determined ini t.ially and every 

48 hours thereafter for the control, solvent control, high; 

medium and lev.; toxicc.nt concentrations. ~'later bath temperature 

was deter.:nined initially and at 4 8 hour intervals subsequent 

to the initiation of the ~est. In additio!l to obtaining tht:! 

above chemical and physical parameters, abnormal behnvi6.ra1 

responses of the test fish \vere noted and reco.rded at 24 hour 

intervals. 

The concentration of toxicant lethal to SO% of the population (LC50 ) and 95t, confidence limits could not be determined 

d~e to the absehce 6f mortalities. Test results were b~sed 

uport nomj :1al concentrations of the t.est material in sof~ reconstituted water. -The nb effect level was determined At the 96 

hourex~osure pericid. This v~lue is GasGd upon the absence of 
96 hour LC50 for HBCD, Lot #990-17, to bluegill sun .... 

·fish is >100.0 mg/1. Test results are presented in Table 1. 

The chemical and physic3.l parameters monitored during the 

are pr.~sented in Table 2. Behavioral observations made during 

the test indicated no observable abnormal beha•Jior in any concentration throughout the test •. 

It should be noted that LCs o values may vary with different species, temperatures and \vater qualities. 
---' 

.. Table 1 Percent I·lo.ctalities and LCs 0 Values 

Client: Vclsico1 Chemical Coq:Joration. 

Test !·lateria.l:. II!3CD;. Lot 11990-17 Test Organism: Dluegill Sunfish 

r:-"'"----,-'--_:__--,-,_:...__.c:.. t !-lor . ..:...i..:...t;:..y..:-,___ ··:···-·-··:···:·:--::--.. -····

Test !·latcrial, Nominal··conc;. 

control I r;o I .32.o 

- -·----·-·· 

I : : f : ; ' ' -----'-

LCn Values 

48 ·nour 96 Hour 

LCs o · mg;l >100.0 > 100.0 >100. 0 

Confidence · ·. . .. · . · . ·. : 

95' Lo·• N .• A. I N.A.±it=N.A .. ·.· · .. ·· . 

. Limi,tS High .·· N.l\o , N.A. ,, 

!LA. = Not avai I able due to absence of mortalities. 

The 9 6 hour observed no effect level is 100.0 P.lg/L 

UNIQf.J EtlV!RONt·1BNTAL· SEnVICES I,' "' ' ', 
--

Initial 

4.8 hour 

96 hour 

:._.J ·-·-·-· ;) 

2 - Physical anc1 Chemical Parameters 

Client: 

Test 

Test 

Specific 

ConcJI.lcti1nce 

· Velsicoi Chcmical.Corporatiori Ili3CD, Lot #990-17 . 

Bluegill Sunfish 

Dilution Water 

_ 

.

1 

Average 

nc 

llanlness 

mg/1 u:>_ CuCO 3 

Total 

Alk<:Jlin

rnq/1 as. Ca

Control 

8.5 

7.3 

4 •. 4 

I Control 

7.50 

6 .. 90 

7.07 

160 20.3±0.7 42 31 

Oxygen, mg/1 

Solvent 

Con l:roJ. 

8.5 

7 .. 2 

2.5 

Solvent 

Control 

7.49 

6.88 

6. 90 

Test N<:lterial, Nomi!'lal Cone .

------- r 

10.0 32 

8.6 . 8. 5 

6. 8 7.0 

--

2.2 I .. 1.9 

p!I 

Tes.t Materiaf, Nomirial Cone. 

10.0 ;2 • 0 

7. 49 7.. 4 9 

6.87 

6. 89 6 •. 85 ··.··j 

UNION 
Al-Tierica.n Public Health Association; 1976. Standard 1>1ethods 

_£or the Examination of \vater and h'astewater, 14th ed. 

New York~ 1193 pp. 

Brauhn, J. L., R. A. Schoettger and L. H. Nueller~ 1975. 

Acquisition and Culture of Research Fish: Rainbow Trout, 

Fathead Mirinows, Channel Catfish and-Dluegills.; EPA-660/3-75-001. 45 pp. 

-Commjttee on Nethods for Toxicity Tests with Aquatic Organisms. 

-- 1975. Nethods for Acute Toxicity 'l'ests withFish, Nacreinvertebrates and Amphibians. EPA-660/3-75-009. 61 PP• 

!-1arking, L. L. and V. K. Dawson. 1973. Toxicity of Quinaldine 

Sulfate to Fish. Invest. Fish Control No. 48. u.s. Fish - Wildl; ~erv., Washington~ D.C. 8 PP• 
\'l. Calmbacher 

Aquatic Toxicologist 

RcportApproved By 

JUgJ.iLdas G. Vilkas 

Section Leader . 

Aquatic Toxicology 

Report Approved By· 

() t ..·. 1\·+f; I.. - 'I - - - ~-'l.,,): <(). \ _t,.- \ .-J. ,( .1-. ,, 

Curt Hutchinson 

Hanager 

UNION CARBIDE CORPOfu~TION 

ENVIRONt,lENTAL SERVICES 

October 3, 197 8 
.· CERTIFICATf·OF AUTHENTICITY 

TriiS IS TO CERTIFY that the microimages appearing on this microfiche are accurate - - - -

and complete reproductions of the records of U.S; Environmental Protection Agency 

documa~ta as delivered_ in the regular course of business for microfilming. 

Data produced f · · ~3 (Month) (Day) 

New York 

(State) 

9o 

(Year) 

0~~ Camera C?perator 

a 

AMTEK. 

·corp