### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 1 of 23 Data Requirement: PMRA Data Code: 9.8.4 (TGAI) or 9.8.6 (EP)

EPA DP Barcode: 4 16756

OECD Data Point: IIA 8.12 (TGAI) and IIIA 10.8.1.1 (EP)

EPA Guideline: 850. 4100

Test material: Nicosulfuron 40 g/L SC Purity: 39.8 %

Common name

Chemical name: IUPAC :

CAS name : 2

- [[[[(4,6

- Dimethoxy

- 2

- pyrimidinyl)amino]carbonyl]amino]sulfonyl]

- N,N

- dimethyl

3

- pyridinecarboxamide

CAS No. : 111991

- 09

- 4

Synonyms : DPX

- V9360 40 g/L SC , DPX

- V9360

- 155 , DPX

- V9360, Nicosulfuron 40 g/L SC, 3

- Pyridinecarboxamide, 2

- - [[[[(4, 6

- Dimethoxy

- 2

- pyrimidinyl)amino]carbonyl]amino]sulfonyl]

- N,N

dimethyl

Primary Reviewer: Dana Worcester Signature:

Senior Scientist, CDM Smith Date: 12 / 17 /1 4

Secondary Reviewer: Teri S. Myers Signature:

Senior Scientist, CDM S mith Date: 0 4 / 03 /1 5

Primary Reviewer: Hannah Yingling Date: 9/18/2015

Biologist, EPA

CITATION : Porch, J.R. and K.H

. Martin. Nicosulfuron (DPX

- V9360) 40 G/L SC: A Greenhouse Study to Investigate

the Effects on Seedling Emergence and Gr owth of Ten Terrestrial Plants Following Soil Exp os ure

. Unpublished

study performed by Wildlife International, Easto n , Maryland and sponsored by E. I. du Pont de Nemours and

Company, Wilmington, Delaware

. DuPont Study Number: DuPont

- 21826

. Study complete d August 27 , 20 0 7

.

DISCLAIMER : This document provides guidance for EPA and PMRA reviewers on how to complete a data

evaluation record after reviewing a scientific study concerning the acute toxicity of a pesticide to terrestrial vascular

plants. It is no t intended to prescribe conditions to any external party for conducting this study nor to establish

absolute criteria regarding the assessment of whether the study is scientifically sound and whether the study satisfies

any applicable data requirements. R eviewers are expected to review and to determine for each study, on a case

- by

case basis, whether it is scientifically sound and provides sufficient information to satisfy applicable data

requirements. Studies that fail to meet any of the conditions may b e accepted, if appropriate; similarly, studies that

meet all of the conditions may be rejected, if appropriate. In sum, the reviewer is to take into account the totality of

factors related to the test methodology and results in determining the acceptabili ty of the study.

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 2 of 23 EXECUTIVE SUMMARY :

The effect of Nicosulf

uron 4 0 g/L SC on the seedling emergence of monocot ( corn, Zea mays ; oat, Avena sativa ;

onion, Allium cepa ; and sorghum

, Sorghum bicol o r ) and dicot ( cucumber, Cucumis sativus ; oilseed rape, Bra ssica

napus ; pea, Pisum sativum ; s oybean, Glycine max ; sugarbeet Beta vulgaris ; and tomato, Lycopersicon esculentum )

crops was studied at a nominal concentration for oilseed rape of 0 (negative), 0.000024, 0.000073, 0.00022,

0.00066, 0.0020, 0.0059, 0.018 and 0.053 lb ai/A ; and 0.00042, 0.00083, 0.0017, 0.0034, 0.0067, 0.013, 0.027 and

0.053 lb ai/A for all others. The measured concentration was 0.053 lb ai/A for corn, oat, onion, sorghum and oilseed

rape; 0.052 lb ai/A for cucumber, pea, sugarbee t and tom ato; and 0.056 lb ai/A for soybean.

The growth medium used in the seedling emergence test was standard soil ( loam, pH 6.2, organic carbon 1.0%). On

day 21 the surviving plants per pot were recorded and cut at soil level for measuring the plant height an d dry weight

The emergence in the negative control ranged from 83 to 95%. The reviewer found significant inhibitions in

emergence for sugarbeet of 29% at 0.00083 lb ai/A treatment (p<0.05; Dunnett’s test). There were no significant

effects at any other treatment level for sugarbeet. There were no significant effects on emergence for any other

species.

The reviewer measured survival based on number planted. Negative control survival based on number planted

ranged from 20 to 100 % . The reviewer found s ignificant inhibitions in survival compared to the negative control for

sugarbeet, with inhibition of 43% at the 0.052 lb ai/A treatment level (p<0.05; Dunnett’s test). There were no

significant inhibitions in survival compared to the negative control for any other species.

There were significant inhibitions in dry weight for all species except corn, oats and pea

. Inhibitions compared to the

negative control in cucumber dry weight of 35% at 0.052 lb ai/A; onion of 42% at the 0.053 lb ai/A ; o ilseed rape of

36 and 65% at the 0.018 and 0.053 lb ai/A ; s orghum of 20

- 68% beginning a t the 0.0017 lb ai/A treatments; s oybean

of 22 and 38% at the 0.027 and 0.056 lb ai/A ; s ugarbeet of 33 and 72% at the 0.027 and 0.052 lb ai/A ; and t omato of

46% at the 0.052 lb ai/ A ( Williams test, p<0.05).

There were significant inhibitions in height for all species except corn, pea and tomato

. The reviewer found

s ignificant decrease in height compared to the negative control for cucumber of 29% at the 0.052 lb ai/A treatment

(D unnetts test, p<0.05). There w ere also s ignificant decrease in oats height compared to the negative control of 27,

35 and 15% at the 0.013, 0.027 and 0.053 lb ai/A treatments ; in onion of 23% at the 0.053 lb ai/A ; in o ilseed rape of

15, 46 and 77% at the 0.0059, 0.018 and 0.053 lb ai/A levels; in s orghum of 19

- 71% beginning a t the 0.0017 lb ai/A

treatments; in s oybean of 14, 10, and 22% at the 0.013, 0.027 and 0.056 lb ai/A ; and in s ugarbeet of 42% at the

0.052 lb ai/A treatments (Williams test, p<0.05).

Based on the reviewers results, t he most sensitive monocot species was sorghum based on dry weight , with NOAEC

and I C 25 values of 0.00083 and 0.000954 lb ai/A, respectively ; and the most sensitive dicot species was oilseed rape

based on height with NOAE C and I C 25 values of 0.00 2 and 0.00 89 lb ai/A, respectively

.

The occurrence of phytotoxic effects increased in severity and prevalence with increasing test concentrations;

symptoms included wilting, stunting, leaf deformation, bleaching, chlorosis, necro sis, and mortality. The visual

response score of 100 indicated normal seedlings and 0 indicated complete mortality. Maximum effects were 35

- 73

for all species with the exception corn and tomato, which had maximum effects of 100 and 93, respectively. Effe cts

were dose

- related.

Maximum Labeled Rate: 0.053 lb ai/A (equivalent to 60 g as/ha)

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 3 of 23 Results Synopsis

Most sensitive monocot: Sorghum, based on dry weight

EC 50 /IC 50 : 0. 00678 lb ai/A 95% C.I.: 0.00462

- 0.00996 lb ai/A

EC 25 /IC 25 : 0.000954 lb ai/ A 95% C.I.: 0.000436

- 0.00185 lb ai/A

EC 05 /IC 05 : 0 .0000567 lb ai/A 95% C.I.: N/A

- 0.00 0401 lb ai/A

NOEC: 0.00083 lb ai/A

Slope: N/A 95% C.I.: N/A

Most sensitive dicot: Oilseed rape , based on height

EC 50 /IC 50 : 0.0207 lb ai/A 95% C.I.: 0. 0168

- 0.0255 lb ai/A

EC 25 /IC 25 : 0.0089 lb ai/A 95% C.I.: 0.00599

- 0.0123 lb ai/A

EC 05 /IC 05 : 0.00264 lb ai/A 95% C.I.: N /A

- 0.00441 lb ai/A

NOEC: 0.00 2 lb ai/A

Slope: N/A 95% C.I.: N/A

Table 1 (Tier II studies). Summary of most sensitive pa rameters by species (lbs ai/A).

Species Endpoint NOEC EC 05 / I C 05 EC 25 / I C 25 EC 50 / I C 50

Corn Dry weight 0.053 0.000636 NC NC

Cucumber Dry weight 0.027 0.000 0 12 0.0105 1.17

Oats Height 0.0067 0.000535 0. 0 329 0.577

Onion Height 0.027 0.00514 0.0535 0.273

Oi lseed Rape Height 0.002 0.00264 0.0089 0.0207

Pea None 0.052 >0.052 >0.052 >0.052

Sorghum Dry weight 0.00083 0.0000567 0.000954 0.00678

Soybean Dry weight 0.013 0.00789 0.0418 0.133

Sugarbeet Dry weight 0.027 0.0118 0.0225 0.0354

Tomato Dry weight 0.0 27 0.0024 0.0179 0.0724

This study is scientifically sound and is classified as acceptable.

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 4 of 23 I. MATERIALS AND METHODS

GUIDELINE FOLLOWED: This study was conducted in compliance with OPPTS 850.4 100 : Seedling

emergence and seedling growth (January 2012) and OECD 208 Guideline

for test of chemicals, Terrestrial Plant Test: Seedling Emergence and

Seedling Growth Test (July 2006)

. The reviewer evaluated the study

methods according to EPA Ecological Effects Test Guidelines, OCSPP

Guideline 850.4100: Se edling Emergence and Seedling Growth. There

were some deficiency and d eviations noted by the reviewer

.

1. The physico

- chemical properties of the test material were not reported

.

The deficiency and d eviations did not have an impact on the acceptability o f this study.

COMPLIANCE: S igned and dated GLP, Quality Assurance and Data Confidenti ality

statements were provided. This study was conducted in compliance with

Good Laboratory Practice Standards as published by USEPA, August 17,

1989, OECD Principle of Good Laboratory Practice ( Paris, 1998 ) ; and

Japan MAFF, October 1, 1999

.

A. MATERIALS:

1. Test Material Nicosulfuron

Description: Not reported

.

Lot No./Batch No. : DPX

- V9360

- 155

Purity: 38.7 %

Stability of compound

under test conditions: Analytical determinations based on measured concentration of the test

material in the initial spray solution yielded a re cover ies of 98

- 105 % of

nominal (n = 4 )

.

(OECD recommends chemical stability in water and light )

Storage cond itions of

test chemicals: T he test material was stored at <40oC

.

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 5 of 23 Table 2. Physical/chemical properties of Nicosulfuron

Parameter Values Comments

Water solubility at 20  ̊ C Not reported

Vapor pressure Not reported

UV absorption Not reported

pKa Not reported

Kow Not reported

2. Test organism :

Monocotyledonous species: Oats ( Avena sativa , Poaceae ), corn ( Zea mays , Poaceae ), onion ( Allium cepa

, Lila ceae) and sorghum ( Sorghum bicolar , Poaceae ) ; EPA recommends four monocots in two families,

including corn.

Dicotyledonous species: Cucumber ( Cucumis sativus ; Cucurbitaceae); Oilseed rape ( Brassica napus

, Brassicaceae ); pea ( Pisum sativum

, Fabaceae ); Soybe an ( Glycine max , Fabaceae); Sugar beet ( Beta

vulgaris ; Chenopodiaceae); and Tomato ( Lycopersicon esculentum , Solanaceae ) ; EPA recommends six

dicots in four families, including soybean and a root crop.

OECD recommends a minimum of three species selected f or testing, at least one from each of the following categories:

Category 1: ryegrass, rice, oat, wheat, and sorghum; Category 2: mustard, rape, radish, turnip, and Chinese cabbage;

Category 3: vetch, mung bean, red clover, fenugreek, lettuce, and cress.

S eed source: Corn and oilseed rape from Pioneer Hi

- Bred International, Inc.; Cucumber from Ferry

- Morse

Meyer Seed Co.; Oat from Gries Seed Farm; Onion from Wannamaker Seeds; Pea and Tomato from Meyer

Seed Co.; Sorghum from N.C. Organics; Soybean from Missou ri Foundation Seeds; and Sugarbeet from

Betaseed Inc.

Prior seed treatment/sterilization: The seeds were not treated with any type of fungicides, insecticides, or

any pesticides.

Historical % germination of seed: Corn, 96%; cucumber, 82%; oat, 97%; oilseed rape, 100%; onion,

>80%; pea, 85%; soybean, 98%; sugarbeet, 90%; and tomato, 90%.

Seed storage, if any: Not reported.

B. STUDY DESIGN:

1. Experimental Conditions

a. Limit test: None.

b. Range

- finding study : None

.

c. Definitive Study

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 6 of 23 Table 3: Experimental Parameters

- Seedling Emergence.

Parameters Seedling Emergence

Details Remarks

Criteria

Duration of the test 21 days

Recommended test duration is 14

- 21 days.

OECD recommends that the test be terminated no

s ooner than 14 days after 50 percent of the control

seedlings have emerged

Number of

seeds/plants/species/

replicate 4 pots (replicates) with 10 seeds

per pot

.

Ten seeds per replicate should be used.

OECD recommends a minimum of five seeds planted

in each replicate within 24 hours of incorporation

of the test substance. All seeds of each species for

each test should be of the same size class. The seed

should not be imbibed.

Number of replicates

Control:

Adjuvant control:

Treate d: 4

N/A

4 Four replicates per dose should be used.

OECD recommends a minimum of four replicates

per treatment

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 7 of 23 Parameters Seedling Emergence

Details Remarks

Criteria

Test concentrations (lb

ai/A)

Nominal:

Measured: A ll species except oilseed rape :

0 (negative), 0. 00042 , 0. 00 08 3

, 0.00 17 , 0.0 0 34 , 0.0 0 67

, 0.0 13

, 0.0 27 and 0.0 53 lb ai/A ;

Oilseed rape:

0 (negative), 0.000024, 0.000073,

0.00022, 0.00066, 0.0020, 0.00 59,

0.018 and 0.053 lb ai/A.

Corn, oat, onion, and sorghum:

0 (negative), 0.00042, 0.00083,

0.0017, 0.0034, 0.00 67, 0.013,

0.027 and 0.053 lb ai/A for corn,

oat, onion, and sorghum;

Cucumber, pea, sugarbeet, and

tomato:

0 (negative), 0 0.00042, 0.00083,

0.0017, 0.0034, 0.0067, 0.013,

0.027and 0.052 lb ai/A ;

Oilseed rape:

0 (negative), 0.000024, 0.000073,

0.00022 , 0.00066, 0.0020, 0.0059,

0.018 and 0.053 lb ai/A;

Soybean

0 (negative), 0.00042, 0.00083,

0.0017, 0.0034, 0.0067, 0.013,

0.027 and 0.056 lb ai/A

. The high dose concentration was measured, all

others were nominal.

Five test conce ntrations should be used with a dose

range of 2X or 3X progression

OECD recommends three concentrations,

preferably with application rates equivalent to 0.0

(control), 1.0, 10.0 and 100 mg substance per kg of

oven

- dried soil.

Method and interval of

anal ytical verification

LOQ:

LOD: Duplicate samples were taken

from the negative control and the

treatment groups for analysis via

HPLC with Ultracarb C8 column

with UV detection ( 240 nm)

. Not reported.

Lowest analytical standard

analyzed

.

Adjuvant (type, percentage,

if used) N/A

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 8 of 23 Parameters Seedling Emergence

Details Remarks

Criteria

Test container (pot)

Size/Volume

Material:

(glass/polystyrene) Pots with diameter of 1 6 cm and

12 cm depth

.

Plastic Non

- porous containers should be used.

OECD recommends that non

- porous plastic or

glazed pot be used.

Growth facility Greenhouse

Method/depth of seeding A template was used to prepare 10

holes and s eeds introduced

manual ly . Seeds were covered

depressing the soil surface

.

Test material application

Application time including

the p lant growth stage

Number of application

Application interval

Method of application A fter planting.

1

N/A

- single application

The test material was applied

using a linear cabinet track

sprayer ( DeVries Research, Teejet

8001 EVS V nozzle, 40 psi and

applied 41 cm from the soil

surface

.

Details of soil used

Geographic location

Depth of soil collection

Soil texture

% sand N/A

N/A

L oam

51 Non

- sterile top soil

.

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 9 of 23 Parameters Seedling Emergence

Details Remarks

Criteria

% silt

% clay

pH:

% organic carbon

CEC

Moisture at 1/3 atm (%) 38

11

6.2

1. 0

7.1

14.6 Soil mixes containing sandy loam, loam, or clay

loam soil with no greater than 2% organic matter

are preferable. Glass beads, rock wool, and 100%

acid washed sand are not preferred.

OECD prefers the soil to be sieved (0.5 cm) to

r emove coarse fragments. Carbon content should

not exceed 1.5% (3% organic matter). Fine particles

(under 20um) makeup should be between 10 and

20%. The recommended pH is between 5.0 and 7.5.

Details of nutrient medium,

if used N/A

Watering regime and

schedules

Water source/type:

Volume applied:

Interval of application:

Method of application: Sub

- irrigation

. Domestic well water

. Daily

. The plants were bottom watered

daily

. EPA prefers that bottom watering be utilized for

seed ling emergence studies so that the chemical is

not leached out of the soil during the test.

Any pest control

method/fertilization, if used None reported

.

Test conditions

Temperature:

Photoperiod:

Light intensity and quality:

Relative humidity : 16

- 31  ̊ C for corn, oat, onion,

sorghum and oilseed rape ; 16

-

30  ̊C for cucumber, pea, sugarbeet

and tomato; 18

- 31oC for soybean

16L:8D

Natural sunlight supplemented

with a rtificial light.

8.8

- 19.1 mol e s/m 2 /day for corn,

oat, onion, sorghum and oilseed

rape; 10.1

- 1 9.1 moles/m 2 /day for

cucumber, pea, sugarbeet and

tomato and 10.7

- 13.3

moles/m 2 /day for soybean

13

- 86% for soybean and 9

- 80 %

for all others EPA prefers that the cold vs warm loving plants be

tested in two separate groups to optimi ze plant

growth.

OECD prefers that the temperature, humidity and

light conditions be suitable for maintaining normal

growth of each species for the test period.

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 10 of 23 Parameters Seedling Emergence

Details Remarks

Criteria

Reference chemical (if

used)

Name:

Concentrations: N/A

Other parameters, if any None

2. Observations:

Table 4: Observation Parameters

- Seedling Emergence.

Parameters Seedling Emergence

Details Remarks

Parameters measured (e.g., number

of germinated seeds, emerged

seedlings, plant height, dry weight

or other endpoints) - Emergen ce

- Survival

- Shoot height

- Total d ry weight

- Phytotoxicity

Measurement technique for each

parameter Emergence and

phytotoxicity were

visually determined.

Survival was defined as

the percent of emerged by

the study author

. Total

plant weight was

es timated measuring the

total dry weight per

replicate following oven

drying.

Observation intervals Each pot was inspected

weekly , emergence and

phytotoxicity assessments

performed. D ry weight

and shoot height were

recorded at study

termination.

Ot her observations, if any N/A

Were raw data included? Yes

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 11 of 23 Phytotoxicity rating system, if used 0 % = complete mortality or

defoliation ; 9 0 % =at the threshold

of visual detection and 100 % =

No injury or effect

.

II. RESULTS and DISCUSSION:

A. INHIBITOR Y EFFECTS:

1. Seedling Emergence:

The emergence in the negative control ranged from 83 to 95%

. The study author reported no significant

inhibitions in emergence for any species at any treatment level (p>0.05 )

. The reviewer found significant

inhibiti ons in emergence for sugarbeet of 29% at 0.00083 lb ai/A treatment (p<0.05; Dunnett ’s test). There

were no significant effects at any other treatment level for sugarbeet . There were no significant effects on

emergence for any other species

.

The study a uthor reported % survived based on number emerged. The study author survival in the negative

control ranged from 89 to 100%. T he study author reported no significant inhibitions in survival for any

species except sugarbeet, which had significant effects at the 0.052 lb ai/A (p<0.05; Jonckheere

- Terpstra

Step

- Down test; inhibitions not reported)

. The reviewer measured survival based on number planted.

Negative control survival based on number planted ranged from 20 to 100 %

. The reviewer found

significant inhibitions in survival compared to the negative control for sugarbeet , with in hibition of 43% at

the 0.052 lb ai/A treatment level (p<0.05; Dunnett’s test). The re were n o significant inhibitions in survival

compared to the negative control for a ny other species

.

The study author found inhibitio ns in dry weight for all species except corn, oats and pea. Cucumber had

significant inhibitions compared to the negative control of up to a maximum of 35% beginning at 0.0067 lb

ai/A; onion inhibitions of 42% at 0.053 lb ai/A; oilseed rape of up to 65% beginning at 0.0059 lb ai/A;

sorghum of up to 68% beginning at 0.0017 lb ai/A, soybean of up to 38% beginning at 0.013 lb ai/A;

sugarbeet of 72% at 0.053 lb ai/A and tomato of up to 46% beginning at 0.013 lb ai/ A (p<0.05; Jonckheere

Terpstra Step

- Down test). The reviewer found significant inhibitions in dry weight for all species except

corn, oat and pea

. The reviewer found were significant inhibitions in dry weight for all species except corn,

oats and pea. Th ere were s ignificant inhibitions compared to the negative control in cucumber dry weight of

35% at 0.052 lb ai/A; onion of 42% at the 0.053 lb ai/A ; o ilseed rape of 36 and 65% at the 0.018 and 0.053

lb ai/A ; s orghum of 20

- 68% beginning a t the 0.0017 lb ai/ A treatments; s oybean of 22 and 38% at the 0.027

and 0.056 lb ai/A ; s ugarbeet of 33 and 72% at the 0.027 and 0.052 lb ai/A ; and t omato of 46% at the 0.052

lb ai/A ( Williams test, p<0.05).

The study author found inhibitions in height for all species except corn, pea and tomato . Cucumber had

significant inhibitions compared to the negative control of up to a maximum of 29 % beginning at 0.0 13 lb

ai/A; oat inhibitions of up to 35% beginning at 0.0067 lb ai/A; onion inhibitions of up to 23 % at 0.0 067 lb

ai/A; oilseed rape of 46 and 77 % at 0.018 and 0.053 lb ai/A ; sorghum of up to 71 % beginning at 0.0017 lb

ai/A, soybean beginning at 0.013 lb ai/A (inhibitions not reported) ; and sugarbeet of 2 and 42 % at 0.027

and 0.052 lb ai/A (p<0.05; Jonckheere

- Terpstra Step

- Down test). The reviewer found significant

inhibitions in height for all species except corn, pea and tomato

. The reviewer found s ignificant decrease in

height compared to the negative control for cucumber of 29% at the 0.052 lb ai/A treatment (Dunnetts test,

p<0.05). There were also s ignificant decrease s in oats height compared to the negative control of 27, 35 and

15% at the 0.013, 0.027 and 0.053 lb ai/A treatments ; in onion of 23% at the 0.053 lb ai/A ; in o ilseed rape

of 15, 46 and 77% at the 0.0059, 0.018 and 0.053 lb ai/A levels; in s orghum of 19

- 71% beginning a t the

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 12 of 23 0.0017 lb ai/A treatments; in s oybean of 14, 10, and 22% at the 0.013, 0.027 and 0.056 lb ai/A ; and in

s ugarbeet of 42% at the 0.052 lb ai/A treatments (Williams test, p<0.05).

Based o n the study author’s results, t he most sensitive monocot species was sorghum based on h eight , with

NOAEC and ER 5 0 values of 0.00 08 and 0.006 3 lb ai/A, respectively ; and t he most sensitive dicot species

was oilseed rape based on height , with NOAEC and E R 5 0 values of 0.0059 and 0.0 204 lb ai/A, respectively

.

The occurrence of phytotoxic effects increased in severity and prevalence with increasing test

concentrations; symptoms included wilting, stunting, leaf deformation, bleaching, chlorosis

, necrosis

, and

m ortality

. The visual response score of 100 indicated normal seedlings and 0 indicated complete mortality.

Maximum effects were 35

- 73 for all species with the exception corn and tomato , which had maximum

effects of 100 and 93, respectively . Effects were d ose

- related.

B. REPORTED STATISTICS:

Emergence, survival, dry weight, and height means and standard deviations were determined for control and

treatment groups. Treatments were compared to the negative control. NOEC was determined using Jonckheere

Terpstra test. EC 25 and EC 50 and their 95% confidence intervals were determined using non

- linear regression

analysis

. All statistical determinations were made with 95% certainty. Nominal concentrations were used for all

analyses.

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 13 of 23 Table 5: Effect of Nicosulfuron on 21

- Day Seedling Emergence

Species Results summary for height (lbs ai/A) *

height (cm) NOEC I C 05 95%CI I C 25 95%CI I C 50 95%CI slope 95%CI

Corn 26.9

- 29 0.05 3 ND N/A >0.05 3 N/A >0.05 3 N/A N/A N/A

C ucumber 1 4.7

- 7.35 0.0067 ND N/A 0.0373 0.00 16

-

0.0533 >0.05 3 N/A N/A N/A

Oats 2 14.8

- 23.1 0.0034 ND N/A 0.0329 0.0018

-

0.6145 >0.05 3 N/A N/A N/A

Onion 3 6.38

- 9.35 0.00 34 ND N/A >0.53 N/A >0.05 3 N/A N/A N/A

Oilseed Rape 4 1.73

- 8.3 0.005 9 ND N/A 0.0088 0.0055

-

0.0142 0.0204 0.0155

-

0.0272 N/A N/A

Pea 8. 8

- 11.1 >0.05 3 ND N/A >0.05 3 N/A >0.05 3 N/A N/A N/A

Sorghum 5 5.23

- 18 0.00 08 3 ND N/A 0.0012 0.0004 2

-

0.0082 0.0063 0.0019

-

0.0214 N/A N/A

Soybean 6 15.4

- 19.7 0.0067 ND N/A 0.0533 0.0000

-

0.0000 >0.05 3 N/A N/A N/A

Sugarbeet 7 3.23

- 6.73 0.0133 ND N/A 0.0401 0.0 212

-

> 0.053 >0.05 3 N/A N/A N/A

Tomato 4.7

- 6.85 0.05 3 ND N/A >0.05 3 N/A >0.05 3 N/A N/A N/A

N D

- No t determined . NC

- Not calculable.

* All statistically significant inhibitions based on Jonckheere

- Terpstra Step

- Down test, p<0.05.

1 Significant in hibition in cucumber height of 21, 12 and 29% at the 0.013, 0.027 and 0.053 lb ai/ A treatments c ompared to the negative control.

2 Significant inhibitions in oat height of 8, 27, 35 and 15% at the 0.0067, 0.013, 0.027 and 0.053 lb ai/A treatments compared to the negat ive

control.

3 Significant i nhibition in onion height of 2, 5, 7 and 23% at the 0.0067, 0.013, 0.027 and 0.053 lb ai/A treatments c ompared to the negative

control.

4 Significant inhibition in oilseed rape height of 46 and 77% at the 0 .018 and 0.053 lb ai/A levels compared to the negative control.

5 Significant i nhibition in sorghum height of 1 8

- 71 % beginning at the 0.0017 lb ai/A treatments c ompared to the negative control.

6 Significant inhibition in soybean height at the 0.013, 0.027 and 0.053 lb ai/A tre atments compared to the negative control (inhibitions not

reported).

7 Significant i nhibition in sugarbeet of 2 and 42 % at the 0.027 and 0.052 lb ai/A treatments compared to the negative control.

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 14 of 23 Table 5a : Effect of Nicosulfuron on 21

- Day Seedling Emer gence

Species Results summary for biomass (lbs ai/A) *

weight ( m g) NOEC I C 05 95%CI I C 25 95%CI I C 50 95%CI slope 95%CI

Corn 1.39

- 1.67 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

C ucumber 1 0.675

- 1.06 0.0034 ND N/A 0.0106 0.0004 2

-

> 0.053 >0.5 3 N/A N/A N/A

Oa ts 0.429

- 0.606 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Onion 2 0.0388

- 0.0773 0.0266 ND N/A 0.0281 0.0199

-

0.0398 >0.053 N/A N/A N/A

Oilseed Rape 3 0.0928

- 0.279 0.0020 ND N/A 0.0099 0.0050

-

0.0198 0.0300 0.0202

-

0.0445 N/A N/A

Pea 1.35

- 1.84 0.05 3 ND N/A >0 .05 3 N/A > 0.05 3 N/A N/A N/A

Sorghum 4 0.168

- 0.519 0.0008 ND N/A 0.0010 0.0004

-

0.0101 0.0068 0.0016

-

0.0287 N/A N/A

Soybean 5 2.32

- 3.75 0.0067 ND N/A 0.0328 0.0144

-

> 0.05 3 >0.05 3 N/A N/A N/A

Sugarbeet 6 0.055

- 0.26 0.0266 ND N/A 0.0223 0.0072

-

> 0.053 0.0355 0.0 186

-

> 0.053 N/A N/A

Tomato 7 0.138

- 0.303 0.0067 ND N/A 0.0181 0.0052

-

> 0.053 >0.05 3 N/A N/A N/A

N D

- No t determined . NC

- Not calculable.

*All statistically significant inhibitions based on Jonckheere

- Terpstra Step

- Down test, p<0.05.

1 S ignificant inhibition in cucumber dry weight of 35, 28, 15 and 35% at the 0.0067, 0.13, 0.027 and 0 .0 5 3 lb ai/A treatments co mpared to the

negative control

. 2 Significant i nhibition in onion dry weight of 42 % at the 0.0 53 lb ai/A treatments compared to the negative control.

3 S ignificant inhibition in oilseed rape dry weight of 22, 36 and 65 % at the 0.0059, 0.018 and 0.053 lb ai/A levels compared to the negative control.

4 Significant i nhibition in sorghum dry weight of 1 9

- 68 % beginning at the 0.0 0 1 7 lb ai/A treatments compared to the negative control

. 5 Significant inhibition in soybean dry weight of 16, 22 and 38 % at the 0.0 13

, 0.0 27 and 0.0 5 3 lb ai/A treatments compared to the negative control

. 6 Significant i nhibition in sugarbeet dry weight of 72 % at the 0.05 3 lb ai/A treat ments c ompared to the negative control.

7 Significant i nhibition in tomato dry weight of 13, 13 and 46% at the 0.013, 0.027 and 0.052 lb ai/A treatments compared to the negative control.

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 15 of 23 Table 5b: Effect of Nicosulfuron on 21

- Day Seedling Emerg ence

Species Results summary for emergence (lbs ai/A)

% NOEC EC 05 95%CI EC 25 95%CI EC 50 95%CI slope 95%CI

Corn 88

- 98 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

C ucumber 55

- 83 0.05 3 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Oats 83

- 95 0.053 ND N/A >0.053 N/ A >0.053 N/A N/A N/A

Onion 85

- 95 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Oilseed Rape 80

- 93 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Pea 88

- 98 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Sorghum 93

- 100 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

So ybean 85

- 98 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Sugarbeet 68

- 90 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Tomato 75

- 90 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

N D

- No t determined . NC

- Not calculable.

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 16 of 23 Table 5c: Effect of Nico su l furon on 21

- Day Seedling Emergence

Species Results summary for survival (lbs ai/A) ; based on # emerge d

% NOEC EC 05 95%CI EC 25 95%CI EC 50 95%CI S lope * 95%CI

Corn ND 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

C ucumber ND 0.05 3 ND N/A >0.05 3 N/A >0.05 3 N/A N/A N/A

O ats ND 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Onion ND 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Oilseed Rape ND 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Pea ND 0.05 3 ND N/A >0.05 3 N/A >0.05 3 N/A N/A N/A

Sorghum ND 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Soybean ND 0.056 3 ND N/A >0.05 3 N/A >0.05 3 N/A N/A N/A

Sugarbeet 1 ND 0.05 3 N D N/A 0.044 0.036

-

0.052 >0.053 N/A N/A N/A

Tomato ND 0.05 3 ND N/A >0.05 3 N/A >0.05 3 N/A N/A N/A

N D

- No t determined . NC

- Not calculable.

1 Significant i nhibition a t the 0.052 lb ai/A treatment compared to the negative control ( Jonckheere

- Terpstra Step

- Down test , p<0.05 ; inhibition not

reported ).

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 17 of 23 Mid

- study emergence

Control Corn Cucumber Oats Onion Oilseed

Rape Pea Sorghum Soybean Sugarbeet Tomato Formulation

Blank

83

- 95 85

- 98 55

- 83 80

- 95 85

- 95 78

- 93 83

- 98 88

- 100 78

- 90 60

- 90 75

- 90 N/A

Plant Injury Index*

Control Corn Cucumber Oats Onion Oilseed

Rape Pea Sorghum Soybean Sugarbeet Tomato Formulation

Blank

0 100 70

- 98 73

- 100 75

- 100 35

- 95 75

- 100 38

- 100 80

- 100 55

- 100 93

- 100 N/A

0 % = Complete mortality ; 100%

= No visual response.

.

C. VERIFICATION OF STATISTICAL RESULTS BY THE REVIEWER:

All analyses were conducted comparing treated to the negative control. These analyses were conducted using

CETIS version 1.8. 7. 12 and backend settings approved for use by EFED on 3 / 25 /1 4

. Data for each endpoint

were tested to determine if their distributions were normal and if their variances were homogeneous using

Shapiro

- Wilk’s and Levene’s tests, respectively. Data that sa tisfied these assumptions were subjected to

Dunnett’s and William’s tests, and data that did not satisfy these assumptions were subjected to the non

parametric MannWhitney

- U and Jonckheere’s tests. Nominal concentrations were used for all analyses except

f or high dose concentrations which were measured

. Linear (survival and emergence) and nonlinear (height and

dry weight) regression models were used to interpret EC/ICx values

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 18 of 23 Table 6: Effect of Nicosulfuron on 21

- Day Seedling Emergence

Species Results su mmary for height (lbs ai/A)

height

(cm) NOEC I C 05 95%CI I C 25 95%CI I C 50 95%CI slope 95%CI

Corn 26.9

- 29 0.053 >0.053 N/A >0.053 N/A >0.053 N/A N/A N/A

C ucumber 1 4.7

- 7.35 0.027 0.000585 N/A

0.00516 0.0363 0.008 4 4

-

0.118 0.638 0.00669

-

60.9 N/A N/A

Oats 2 14.8

- 23.1 0.0067 0.000535 N/A

0.00279 0.0329 0.0135

-

0.072 0.577 0.0415

-

8.02 N/A N/A

Onion 3 6.38

- 9.35 0.027 0.00514 N/A

0.0139 0.0535 0.0235

-

0.104 0.273 0.0274

-

2.73 N/A N/A

Oilseed Rape 4 1.73

- 8.3 0.002 0.00264 N/A

0.00441 0.0089 0.00599

-

0.0123 0.0207 0.01 68

-

0.0255 N/A N/A

Pea 8.8

- 11.1 0.052 >0.052 N/A >0.052 N/A >0.052 N/A N/A N/A

Sorghum 5 5.23

- 18 0.00083 0.000102 N/A

0.000431 0.0011

6 0.000674

-

0.00186 0.00628 0.0047

-

0.00839 N/A N/A

Soybean 6 15.4

- 19.7 0.0067 0.0000707 N/A

0.00505 2.2 0.000107

-

683 >0.056 N/A N/A N/A

Sugarbeet 7 3.23

- 6.73 0.027 0.00335 N/A

0.0106 0.0231 0.0111

-

0.0412 0.0882 0.0264

-

0.294 N/A N/A

Tomato 4.7

- 6.85 0.052 >0.052 N/A >0.052 N/A >0.052 N/A N/A N/A

N D

- No t determined . NC

- Not calculable.

1 Significant decrease in cucumber height, inhibition of 29% at the 0.052 lb ai/A treatments compared to the negative control ( Dunnett s test,

p<0.05).

2 Significant decrease in oats height, inhibition of 27, 35 and 15% at the 0.013, 0.027 and 0.053 lb ai/A treatments compared to the negative contr ol

(Williams test, p<0.05).

3 Significant decrease in onion height, inhibition of 23% at the 0.053 lb ai/A treatments compared to the negative control (W illiams test, p<0.05).

4 Significant decrease in oilseed rape height, inhibitions of 15, 46 and 77% at the 0.0059, 0.018 and 0.053 lb ai/A levels compared to the negative

control ( Williams test, p<0.05).

5 Significant decrease in sorghum height, inhibition of 19

- 71% beginning at the 0.0017 lb ai/A treatments compared to the negative control

(Williams test, p<0.05).

6 Significant decrease in soybean height, inhibition of 14, 10, and 22% at the 0.013, 0.027 and 0.056 lb ai/A treatments comp ared to the negative

control (Williams test, p<0.05).

7 Significant decrease in sugarbeet h eight, inhibition of 42% at th e 0.052 lb ai/A treatments compared to the negative control (Williams test,

p<0.05).

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 19 of 23 Table 6 a : Effect of Nicosulfuron on 21

- Day Seedling Emergence

Species Results summary for biomass (lbs ai/A)

weight ( m g) NOEC I C 05 95%CI I C 25 95%CI I C 50 95%CI slop e 95%CI

Corn 1.39

- 1.67 0.053 0.000636 N/A

0.00029 NC N/A NC N/A N/A N/A

C ucumber 1 0.675

- 1.06 0.027 0.000012 N/A

0. 00306 0.0105 0.00156

-

0.0525 1.17 0.000936

-

1470 N/A N/A

Oats 0.429

- 0.606 0.053 >0.053 N/A >0.053 N/A >0.053 N/A N/A N/A

Onion 2 0.0388

- 0.077 3 0.027 >0.053 N/A >0.053 N/A >0.053 N/A N/A N/A

Oilseed Rape 3 0.0928

- 0.279 0.0059 0.00206 N/A

0.00488 0.01 0.00499

-

0.0171 0.0301 0.021

-

0.0431 N/A N/A

Pea 1.35

- 1.84 0.052 >0.052 N/A >0.052 N/A >0.052 N/A N/A N/A

Sorghum 4 0.168

- 0.519 0.00083 0.0000567 N/ A

0.000401 0.000954 0.000436

-

0.00185 0.00678 0.00462

-

0.00996 N/A N/A

Soybean 5 2.32

- 3.75 0.013 0.00789 N/A

0.0193 0.0418 0.0239

-

0.0659 0.133 0.0341

-

0.518 N/A N/A

Sugarbeet 6 0.055

- 0.26 0.013 0.0118 N/A

0.0203 0.0225 0.008

-

0.0346 0.0354 0.0264

-

0.0473 N/A N/ A

Tomato 7 0.138

- 0.303 0.027 0.0024 N/A

0.00678 0.0179 0.00964

-

0.0299 0.0724 0.0318

-

0.165 N/A N/A

N D

- No t determined . NC

- Not calculable.

1 Significant decrease in cucumber dry weight, inhibition of 35% at the 0.052 lb ai/A treatments compared to the nega tive control (Williams test,

p<0.05).

2 Significant decrease in onion dry weight, inhibition of 42% at the 0.053 lb ai/A treatments compared to the negative contro l (Williams test,

p<0.05).

3 Significant decrease in oilseed rape dry weight, inhibitions of 36 and 65% at the 0.018 and 0.053 lb ai/A levels compared to the negative control

(Williams test, p<0.05).

4 Significant decrease in sor ghum dry weight, inhibition of 20

- 68% beginning at the 0.0017 lb ai/A treatments compared to the negative control

(Will iams test, p<0.05).

5 Significant decrease in soybean dry weight, inhibition of 22 and 38% at the 0.027 and 0.056 lb ai/A treatments compared to the negative control

(Williams test, p<0.05);

6 Significant decrease in sugarbeet dry weight, inhibition of 33 and 72% at the 0.027 and 0.052 lb ai/A treatments compared to the negative control

(Williams test, p<0.05).

7 Significant decrease in tomato dry weight, inhibition of 46% at the 0.052 lb ai/A treatments compared to the negative contr ol Williams test,

p<0. 05).

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 20 of 23 Table 6b: Effect of Nicosulfuron on 21

- Day Seedling Emergence

Species Results summary for emergence (lbs ai/A)

% NOEC EC 05 95%CI EC 25 95%CI EC 50 95%CI slope 95%CI

Corn 88

- 98 0.053 >0.053 N/A >0.053 N/A >0.053 N/A N/A N/A

C ucumber 55

- 83 0.052 >0 .052 N/A >0.052 N/A >0.052 N/A N/A N/A

Oats 83

- 95 0.053 >0.053 N/A >0.053 N/A >0.053 N/A N/A N/A

Onion 85

- 95 0.053 >0.053 N/A >0.053 N/A >0.053 N/A N/A N/A

Oilseed Rape 80

- 93 0.053 >0.053 N/A >0.053 N/A >0.053 N/A N/A N/A

Pea 85

- 98 0.052 >0.052 N/A >0. 052 N/A >0.052 N/A N/A N/A

Sorghum 93

- 100 0.053 >0.053 N/A >0.053 N/A >0.053 N/A N/A N/A

Soybean 85

- 98 0.056 >0.056 N/A >0.056 N/A >0.056 N/A N/A N/A

Sugarbeet 1 60

- 90 0.00042 N/C N/A N/C N/A N/C N/A N/A N/A

Tomato 83

- 90 0.052 >0.052 N/A >0.052 N/A >0.0 52 N/A N/A N/A

N D

- No t determined . NC

- Not calculable

. 1 There was a significant inhibition of 29% in sugarbeet dry weight at the 0.00083 lb ai/A level (Dunnett’s test, p<0.05).

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 21 of 23 Table 6c: Effect of Nicosulfuron on 21

- Day Seedling Emergence

Species Results summary for survival (lbs ai/A) ; based on # planted

% NOEC EC 05 95%CI EC 25 95%CI EC 50 95%CI slope 95%CI

Corn 88

- 98 0.053 >0.053 N/A >0.053 N/A >0.053 N/A N/A N/A

C ucumber 50

- 78 0.052 >0.052 N/A >0.052 N/A >0.052 N/A N/A N/A

Oats 83

- 95 0.053 >0 .053 N/A >0.053 N/A >0.053 N/A N/A N/A

Onion 85

- 95 0.053 >0.053 N/A >0.053 N/A >0.053 N/A N/A N/A

Oilseed Rape 80

- 88 0.053 >0.053 N/A >0.053 N/A >0.053 N/A N/A N/A

Pea 85

- 98 0.052 >0.052 N/A >0.052 N/A >0.052 N/A N/A N/A

Sorghum 90

- 98 0.053 >0.053 N/A >0.053 N/A >0.053 N/A N/A N/A

Soybean 80

- 98 0.056 >0.056 N/A >0.056 N/A >0.056 N/A N/A N/A

Sugarbeet 1 43

- 90 0.027 NC N/A NC N/A NC N/A N/A N/A

Tomato 93

- 90 0.052 >0.052 N/A >0.052 N/A >0.052 N/A N/A N/A

N D

- No t determined . NC

- Not calculable

. 1 Signifi cant decrease in sugarbeet survival, inhibition of 43% at the 0.052 lb ai/A treatments compared to the negative control ( Dunnett’s test,

p<0.05).

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 22 of 23 Mid

- study emergence

Control Corn Cucumber Oats Onion Oilseed

Rape Pea Sorghum Soybean Sugarbeet Tomato Fo rmulation

Blank

Not reported N/A

Plant Injury Index*

Control Corn Cucumber Oats Onion Oilseed

Rape Pea Sorghum Soybean Sugarbeet Tomato Formulation

Blank

0 100 70

- 98 73

- 100 75

- 100 35

- 95 75

- 100 38

- 100 80

- 100 55

- 100 93

- 100 N/A

0%

= Complete mortality ; 10 0%=No visual response

.

Monocot

Most sensitive monocot: Sorghum , based on dry weight

EC 50 /IC 50 : 0. 00678 lb ai/A 95% C.I.: 0.00462

- 0.00996 lb ai/A

EC 25 /IC 25 : 0.000954 lb ai/A 95% C.I.: 0.000436

- 0.00185 lb ai/A

EC 05 /IC 05 : 0 .0000567 lb ai/A 9 5% C.I.: N/A

- 0.00 0401 lb ai/A

NOEC: 0.00083 lb ai/A

Slope: N/A 95% C.I.: N/A

Most sensitive dicot: Oilseed rape

, based on height

EC 50 /IC 50 : 0.0207 lb ai/A 95% C.I.: 0.0168

- 0.0255 lb ai/A

EC 25 /IC 25 : 0.0089 lb ai/A 95% C.I.: 0.00599

- 0.0123 lb ai/A

EC 05 /IC 05 : 0.00264 lb ai/A 95% C.I.: N /A

- 0.00441 lb ai/A

NOEC: 0.00 2 lb ai/A

Slope: N/A 95% C.I.: N/A

D. STUDY DEFICIENCIES:

1. The physico

- chemical properties of the test material were not reported

.

E. REVIEWER ’ S COMMENTS:

The reviewer and study author results are in general agreement. The study author reported the most

sensitive monocot species was so r ghum based on height , with NOAEC and I R 5 0 values of 0.0008 and

0.006 8 lb ai/A, respectively; and the most sensitive dicot s pecies was oilseed rape based on height , with

NOAEC and I R 5 0 values of 0.0059 and 0.0 155 lb ai/A, respectively. In comparison, based on the reviewers

### Data Evaluation Record on the Acute Toxicity of Nicosulfuron to Terre strial Vascular

Plants: Seedling Emergence

PMRA Submission Number {..............} EPA MRID Number 49169704

Page 23 of 23 results, the most sensitive monocot species was sorghum based on dry weight, with NOAEC and IC 25 values

o f 0.00083 and 0.00 0954 lb ai/A, respectively; and the most sensitive dicot species was oilseed rape based

on height , with NOAEC and I C 25 values of 0.002 and 0. 0089 lb ai/A, respectively. The study author based

most sensitive species results on the EC 50 va lue, while the reviewer based the most sensitive species on IC 25

. Also, i f the study author had used their calculated I C 25 for sorghum, dry weight would have been the most

sensitive endpoint. These differences most likely resulted in the difference in the monocot endpoint of

height vs. dry weight for sorghum. T he reviewer’s results are presented in the Executive Summary and

Conclusions sections of this DER.

Th e in

- life portion of this study was initiated on March 13, 2007 to Ma y 24, 200 7

.

F. CONCLUSIO NS:

This study is scientifically sound and is classified as acceptable

. Th e most sensitive monocot species was

sorghum based on dry weight , with NOAEC and I C 25 values of 0.00083 and 0.00 0954 lb ai/A, respectively ;

and the most sensitive dicot species w as oilseed rape based on height , with NOAEC and I C 25 values of

0.002 and 0.00 89 lb ai/A, respectively

.

Most sensitive monocot and IC 25 : S orghum (dry weight, 0.00 0954 lb ai/A).

Most sensitive dicot and I C 25 : Oilseed rape ( height

, 0.00 89 lb ai/A).

III

. REFERENCES :

1. 

### U.S. Environmental Protection Agency, Series 850

- Ecological Effects Test Guidelines ( draft ), PPPTS

Number 850.4100: Terrestrial Plant Toxicity, Tier I (Seedling Emergence). 1996

.

2. 

### U.S. Environmental Protection Agency, Series 850

- Ecological Effects Test Guidelines ( draft ), PPPTS

Number 850.4225: Terrestrial Plant Toxicity, Tier II (Seedling Emergence). 1996

.

3. 

### Bruce, Robert D. and Donald J. Versteeg. 1992 A Statistical Procedure for Modeling Continuous

Toxicity Data. Environmental Toxicology and Chemistry . 11:1485

- 1494

.

4. 

### U.S. Environmental Protection Agency, “Pesticide Assessment Guidelines, Subdivision J, Hazard

Evaluation Nontarget Plants, Series 122

- 1 and 123

- 1”, 22 October 1982

.

5. 

### OECD , OECD: Guideline for the T esting of C hemicals, Guidelin e 208. Terrestrial Plant Test:

Seedling Emergence and Seedling Growth Test, OECD, July 2006.

6. 

### Frans, R. E. and Talbert, R. E., Design of Fields Experiment and the Measurements and Analysis of

Plant Responses. Pages 15

- 23 in B. Truelove, ed. Research Method s in Weed Science. Southern Weed

Science Society, Auburn University, Alabama, 1977.

7. 

### SAS Institute, Inc. 1999

. SAS/STAT User’s Guide, Version 8. Cary, NC, SAS Institute, Inc.