13339 Hagers Ferry Road

Huntersville, NC 28078-7929

McGuire Nuclear Complex - MG03A2

Phone: 980-875-5245 Fax: 980-875-4349

Order Summary Report

Analytical Laboratory

Order Number: J12080128

Project Name: Somerville Waste Water

Lab Contact: Jason C Perkins

Date: 8/24/2012

Customer Address: 253 Plant Allen Road

Customer Name(s): BillK-RonL--RobnJ-DonS-RayL

Belmont, NC 28012

Phone: 980-875-5348

Report Authorized By:

(Signature)

Program Comments:

Please contact the Program Manager (Jason C Perkins) with any questions regarding this report.

Data Flags & Calculations:

Any analytical tests or individual analytes within a test flagged with a Qualifier indicate a deviation from the method quality 

system or quality control requirement. The qualifier description is found at the end of the Certificate of Analysis (sample results) 

under the qualifiers heading. All results are reported on a dry weight basis unless otherwise noted. Subcontracted data 

included on the Duke Certificate of Analysis is to be used as information only. Certified vendor results can be found in the 

subcontracted lab final report. Duke Energy Analytical Laboratory subcontracts analyses to other vendor laboratories that have 

been qualified by Duke Energy to perform these analyses except where noted.

Data Package:

This data package includes analytical results that are applicable only to the samples described in this narrative. An estimation of 

the uncertainty of measurement for the results in the report is available upon request. This report shall not be reproduced, except 

in full, without the written consent of the Analytical Laboratory. Please contact the Analytical laboratory with any questions. The 

order of individual sections within this report is as follows:

Job Summary Report, Sample Identification, Technical Validation of Data Package, Analytical Laboratory Certificate of Analysis, 

Analytical Laboratory QC Reports, Sub-contracted Laboratory Results, Customer Specific Data Sheets, Reports & 

Documentation, Customer Database Entries, Test Case Narratives, Chain of Custody (COC)

Certification:

The Analytical Laboratory holds the following State Certifications : North Carolina (DENR) Certificate #248, South Carolina 

(DHEC) Laboratory ID # 99005. Contact the Analytical Laboratory for definitive information about the certification status of 

specific methods. 

Page 1 of 40
Sample ID's & Descriptions:

Sample ID Plant/Station

Collection 

Date and Time Collected By Sample Description

2012017083 ALLEN 06-Aug-12 8:03 AM Jeff Womack FGD Purge Eff 

2012017084 ALLEN 06-Aug-12 8:07 AM Jeff Womack EQ Tank Eff 

2012017085 ALLEN 06-Aug-12 8:11 AM Jeff Womack BioReactor 1 Inf 

2012017086 ALLEN 06-Aug-12 3:31 PM Jeff Womack BioReactor 1 Inf BLANK 

2012017087 ALLEN 06-Aug-12 8:20 AM Jeff Womack BioReactor 2 Inf 

2012017088 ALLEN 06-Aug-12 3:35 PM Jeff Womack BioReactor 2 Inf BLANK 

2012017089 ALLEN 06-Aug-12 8:15 AM Jeff Womack BioReactor 2 Eff 

2012017090 ALLEN 06-Aug-12 3:32 PM Jeff Womack BioReactor 2 Eff BLANK 

2012017091 ALLEN 06-Aug-12 10:03 AM Jeff Womack Filter Blk 

9 Total Samples

Page 2 of 40
COC and .pdf report are in agreement with sample totals 

and analyses (compliance programs and procedures).

All Results are less than the laboratory reporting limits.

All laboratory QA/QC requirements are acceptable.

Yes No

Technical Validation Review

Checklist:

Yes No

Yes No

Report Sections Included:

Job Summary Report Sub-contracted Laboratory Results

Sample Identification Customer Specific Data Sheets, Reports, & Documentation

Technical Validation of Data Package Customer Database Entries

Analytical Laboratory Certificate of Analysis

Analytical Laboratory QC Report

Chain of Custody

Reviewed By: DataBase Administrator Date: 8/24/2012

Electronic Data Deliverable (EDD) Sent Separately

Page 3 of 40
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12080128

2012017083

Collection Date: 06-Aug-12 8:03 AM

Site: FGD Purge Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY - (Analysis Performed by Prism Labs)

Vendor Parameter Complete Vendor Method V_PRISM

INORGANIC IONS BY IC

Bromide 14 mg/L 5 50 EPA 300.0 8/13/2012 5:25:00 PM JAHERMA

Chloride 2400 mg/L 50 500 EPA 300.0 8/13/2012 5:25:00 PM JAHERMA

Sulfate 1600 mg/L 50 500 EPA 300.0 8/13/2012 5:25:00 PM JAHERMA

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 31.2 ug/L 2.5 50 EPA 245.1 8/16/2012 1:58:11 PM AGIBBS

DISSOLVED METALS BY ICP

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 8/13/2012 12:50:00 PM MHH7131

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 243 mg/L 0.5 10 EPA 200.7 8/13/2012 11:01:00 AM MHH7131

Calcium (Ca) 1750 mg/L 0.1 10 EPA 200.7 8/13/2012 11:01:00 AM MHH7131

Iron (Fe) 93.5 mg/L 0.1 10 EPA 200.7 8/13/2012 11:01:00 AM MHH7131

Lithium (Li) 0.156 mg/L 0.05 10 EPA 200.7 8/13/2012 11:01:00 AM MHH7131

Magnesium (Mg) 738 mg/L 0.05 10 EPA 200.7 8/13/2012 11:01:00 AM MHH7131

Manganese (Mn) 3.26 mg/L 0.05 10 EPA 200.7 8/13/2012 11:01:00 AM MHH7131

Potassium (K) 36.6 mg/L 1 10 EPA 200.7 8/13/2012 11:01:00 AM MHH7131

Sodium (Na) 32.6 mg/L 0.5 10 EPA 200.7 8/13/2012 11:01:00 AM MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) 721 ug/L 10 10 EPA 200.8 8/14/2012 1:39:00 PM DJSULL1

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 127 ug/L 10 10 EPA 200.8 8/14/2012 12:23:00 PM DJSULL1

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 8/14/2012 12:23:00 PM DJSULL1

Chromium (Cr) 166 ug/L 10 10 EPA 200.8 8/14/2012 12:23:00 PM DJSULL1

Copper (Cu) 119 ug/L 10 10 EPA 200.8 8/14/2012 12:23:00 PM DJSULL1

Nickel (Ni) 202 ug/L 10 10 EPA 200.8 8/14/2012 12:23:00 PM DJSULL1

Selenium (Se) 792 ug/L 10 10 EPA 200.8 8/14/2012 12:23:00 PM DJSULL1

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 8/14/2012 12:23:00 PM DJSULL1

Zinc (Zn) 250 ug/L 10 10 EPA 200.8 8/14/2012 12:23:00 PM DJSULL1

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

TOTAL DISSOLVED SOLIDS

TDS 10000 mg/L 200 1 SM2540C 8/15/2012 4:29:00 PM TJA7067

TOTAL SUSPENDED SOLIDS

TSS 2200 mg/L 250 1 SM2540D 8/13/2012 2:26:00 PM TJA7067

Page 4 of 40
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12080128

2012017083

Collection Date: 06-Aug-12 8:03 AM

Site: FGD Purge Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

2012017084

Collection Date: 06-Aug-12 8:07 AM

Site: EQ Tank Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 21.5 ug/L 2.5 50 EPA 245.1 8/16/2012 2:00:35 PM AGIBBS

DISSOLVED METALS BY ICP

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 8/13/2012 12:54:00 PM MHH7131

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 255 mg/L 0.5 10 EPA 200.7 8/13/2012 11:05:00 AM MHH7131

Calcium (Ca) 1900 mg/L 0.1 10 EPA 200.7 8/13/2012 11:05:00 AM MHH7131

Iron (Fe) 65.6 mg/L 0.1 10 EPA 200.7 8/13/2012 11:05:00 AM MHH7131

Lithium (Li) 0.143 mg/L 0.05 10 EPA 200.7 8/13/2012 11:05:00 AM MHH7131

Magnesium (Mg) 721 mg/L 0.05 10 EPA 200.7 8/13/2012 11:05:00 AM MHH7131

Manganese (Mn) 2.19 mg/L 0.05 10 EPA 200.7 8/13/2012 11:05:00 AM MHH7131

Potassium (K) 34.9 mg/L 1 10 EPA 200.7 8/13/2012 11:05:00 AM MHH7131

Sodium (Na) 37.0 mg/L 0.5 10 EPA 200.7 8/13/2012 11:05:00 AM MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) 610 ug/L 10 10 EPA 200.8 8/14/2012 1:42:00 PM DJSULL1

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 87.9 ug/L 10 10 EPA 200.8 8/14/2012 12:26:00 PM DJSULL1

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 8/14/2012 12:26:00 PM DJSULL1

Chromium (Cr) 123 ug/L 10 10 EPA 200.8 8/14/2012 12:26:00 PM DJSULL1

Copper (Cu) 88.2 ug/L 10 10 EPA 200.8 8/14/2012 12:26:00 PM DJSULL1

Nickel (Ni) 141 ug/L 10 10 EPA 200.8 8/14/2012 12:26:00 PM DJSULL1

Selenium (Se) 779 ug/L 10 10 EPA 200.8 8/14/2012 12:26:00 PM DJSULL1

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 8/14/2012 12:26:00 PM DJSULL1

Zinc (Zn) 172 ug/L 10 10 EPA 200.8 8/14/2012 12:26:00 PM DJSULL1

2012017085

Collection Date: 06-Aug-12 8:11 AM

Site: BioReactor 1 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY - (Analysis Performed by Prism Labs)

Vendor Parameter Complete Vendor Method V_PRISM

Page 5 of 40
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12080128

2012017085

Collection Date: 06-Aug-12 8:11 AM

Site: BioReactor 1 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

DISSOLVED METALS BY ICP

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 8/13/2012 12:58:00 PM MHH7131

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 256 mg/L 0.5 10 EPA 200.7 8/13/2012 11:09:00 AM MHH7131

Calcium (Ca) 1800 mg/L 0.1 10 EPA 200.7 8/13/2012 11:09:00 AM MHH7131

Iron (Fe) < 0.1 mg/L 0.1 10 EPA 200.7 8/13/2012 11:09:00 AM MHH7131

Lithium (Li) 0.081 mg/L 0.05 10 EPA 200.7 8/13/2012 11:09:00 AM MHH7131

Magnesium (Mg) 639 mg/L 0.05 10 EPA 200.7 8/13/2012 11:09:00 AM MHH7131

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 8/13/2012 11:09:00 AM MHH7131

Potassium (K) 19.8 mg/L 1 10 EPA 200.7 8/13/2012 11:09:00 AM MHH7131

Sodium (Na) 39.4 mg/L 0.5 10 EPA 200.7 8/13/2012 11:09:00 AM MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) 624 ug/L 10 10 EPA 200.8 8/14/2012 1:46:00 PM DJSULL1

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 10 ug/L 10 10 EPA 200.8 8/14/2012 12:29:00 PM DJSULL1

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 8/14/2012 12:29:00 PM DJSULL1

Chromium (Cr) 15.6 ug/L 10 10 EPA 200.8 8/14/2012 12:29:00 PM DJSULL1

Copper (Cu) < 10 ug/L 10 10 EPA 200.8 8/14/2012 12:29:00 PM DJSULL1

Nickel (Ni) < 10 ug/L 10 10 EPA 200.8 8/14/2012 12:29:00 PM DJSULL1

Selenium (Se) 695 ug/L 10 10 EPA 200.8 8/14/2012 12:29:00 PM DJSULL1

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 8/14/2012 12:29:00 PM DJSULL1

Zinc (Zn) < 10 ug/L 10 10 EPA 200.8 8/14/2012 12:29:00 PM DJSULL1

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

2012017086

Collection Date: 06-Aug-12 3:31 PM

Site: BioReactor 1 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

Page 6 of 40
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12080128

2012017086

Collection Date: 06-Aug-12 3:31 PM

Site: BioReactor 1 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

2012017087

Collection Date: 06-Aug-12 8:20 AM

Site: BioReactor 2 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY - (Analysis Performed by Prism Labs)

Vendor Parameter Complete Vendor Method V_PRISM

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

DISSOLVED METALS BY ICP

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 8/13/2012 1:02:00 PM MHH7131

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 259 mg/L 0.5 10 EPA 200.7 8/13/2012 11:13:00 AM MHH7131

Calcium (Ca) 1830 mg/L 0.1 10 EPA 200.7 8/13/2012 11:13:00 AM MHH7131

Iron (Fe) 0.164 mg/L 0.1 10 EPA 200.7 8/13/2012 11:13:00 AM MHH7131

Lithium (Li) 0.088 mg/L 0.05 10 EPA 200.7 8/13/2012 11:13:00 AM MHH7131

Magnesium (Mg) 646 mg/L 0.05 10 EPA 200.7 8/13/2012 11:13:00 AM MHH7131

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 8/13/2012 11:13:00 AM MHH7131

Potassium (K) 33.2 mg/L 1 10 EPA 200.7 8/13/2012 11:13:00 AM MHH7131

Sodium (Na) 39.5 mg/L 0.5 10 EPA 200.7 8/13/2012 11:13:00 AM MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) 41.3 ug/L 10 10 EPA 200.8 8/14/2012 1:49:00 PM DJSULL1

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 10 ug/L 10 10 EPA 200.8 8/14/2012 12:32:00 PM DJSULL1

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 8/14/2012 12:32:00 PM DJSULL1

Chromium (Cr) < 10 ug/L 10 10 EPA 200.8 8/14/2012 12:32:00 PM DJSULL1

Copper (Cu) < 10 ug/L 10 10 EPA 200.8 8/14/2012 12:32:00 PM DJSULL1

Nickel (Ni) < 10 ug/L 10 10 EPA 200.8 8/14/2012 12:32:00 PM DJSULL1

Selenium (Se) 44.9 ug/L 10 10 EPA 200.8 8/14/2012 12:32:00 PM DJSULL1

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 8/14/2012 12:32:00 PM DJSULL1

Zinc (Zn) < 10 ug/L 10 10 EPA 200.8 8/14/2012 12:32:00 PM DJSULL1

Page 7 of 40
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12080128

2012017087

Collection Date: 06-Aug-12 8:20 AM

Site: BioReactor 2 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

2012017088

Collection Date: 06-Aug-12 3:35 PM

Site: BioReactor 2 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

2012017089

Collection Date: 06-Aug-12 8:15 AM

Site: BioReactor 2 Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY - (Analysis Performed by Prism Labs)

Vendor Parameter Complete Vendor Method V_PRISM

INORGANIC IONS BY IC

Bromide 16 mg/L 5 50 EPA 300.0 8/13/2012 4:32:00 PM JAHERMA

Chloride 3300 mg/L 50 500 EPA 300.0 8/13/2012 4:32:00 PM JAHERMA

Sulfate 1800 mg/L 50 500 EPA 300.0 8/13/2012 4:32:00 PM JAHERMA

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

DISSOLVED METALS BY ICP

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 8/13/2012 1:06:00 PM MHH7131

Page 8 of 40
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12080128

2012017089

Collection Date: 06-Aug-12 8:15 AM

Site: BioReactor 2 Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 257 mg/L 0.5 10 EPA 200.7 8/13/2012 11:16:00 AM MHH7131

Calcium (Ca) 1820 mg/L 0.1 10 EPA 200.7 8/13/2012 11:16:00 AM MHH7131

Iron (Fe) 0.266 mg/L 0.1 10 EPA 200.7 8/13/2012 11:16:00 AM MHH7131

Lithium (Li) 0.092 mg/L 0.05 10 EPA 200.7 8/13/2012 11:16:00 AM MHH7131

Magnesium (Mg) 653 mg/L 0.05 10 EPA 200.7 8/13/2012 11:16:00 AM MHH7131

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 8/13/2012 11:16:00 AM MHH7131

Potassium (K) 33.8 mg/L 1 10 EPA 200.7 8/13/2012 11:16:00 AM MHH7131

Sodium (Na) 36.2 mg/L 0.5 10 EPA 200.7 8/13/2012 11:16:00 AM MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) 10.6 ug/L 5 5 EPA 200.8 8/14/2012 1:52:00 PM DJSULL1

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 5 ug/L 5 5 EPA 200.8 8/14/2012 12:35:00 PM DJSULL1

Cadmium (Cd) < 5 ug/L 5 5 EPA 200.8 8/14/2012 12:35:00 PM DJSULL1

Chromium (Cr) < 5 ug/L 5 5 EPA 200.8 8/14/2012 12:35:00 PM DJSULL1

Copper (Cu) < 5 ug/L 5 5 EPA 200.8 8/14/2012 12:35:00 PM DJSULL1

Nickel (Ni) < 5 ug/L 5 5 EPA 200.8 8/14/2012 12:35:00 PM DJSULL1

Selenium (Se) 9.07 ug/L B 5 5 EPA 200.8 8/14/2012 12:35:00 PM DJSULL1

Silver (Ag) < 5 ug/L 5 5 EPA 200.8 8/14/2012 12:35:00 PM DJSULL1

Zinc (Zn) < 5 ug/L 5 5 EPA 200.8 8/14/2012 12:35:00 PM DJSULL1

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

2012017090

Collection Date: 06-Aug-12 3:32 PM

Site: BioReactor 2 Eff BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

Page 9 of 40
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12080128

2012017091

Collection Date: 06-Aug-12 10:03 AM

Site: Filter Blk 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

DISSOLVED METALS BY ICP

Manganese (Mn) < 0.005 mg/L 0.005 1 EPA 200.7 8/13/2012 1:10:00 PM MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) 1.21 ug/L B 1 1 EPA 200.8 8/14/2012 1:24:00 PM DJSULL1

Qualifiers:

B Target analyte detected in method blank at or above the reporting limit. Target analyte concentration in 

sample is less than 10X the concentration in the method blank. Analyte concentration in sample could be 

due to contamination.

Page 10 of 40
 

August 16, 2012 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

(704) 875-5245 

Project: Allen Shay/MillerCreek (LIMS #J12080128) 

Dear Mr. Perkins, 

Attached is the report associated with four (4) aqueous samples submitted for selenium 

speciation analysis on August 7, 2012. The samples were received in a sealed cooler at -0.3°C 

on August 8, 2012. Selenium speciation analysis was performed via ion chromatography 

inductively coupled plasma collision reaction cell mass spectrometry (IC-ICP-CRC-MS). Any 

issues associated with the analysis are addressed in the following report. 

If you have any questions, please feel free to contact me at your convenience. 

 Sincerely, 

 

 Russell Gerads 

 Vice President 

 Applied Speciation and Consulting, LLC 

 

Page 11 of 40
Applied Speciation and Consulting, LLC 

Report prepared for: 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

Project: Allen Shay/MillerCreek (LIMS #J12080128) 

August 16, 2012 

1. Sample Reception 

Four (4) aqueous samples in 125mL HDPE bottles (provided by Applied Speciation and 

Consulting) were submitted for selenium speciation analysis on August 7, 2012. The 

samples were received on August 8, 2012 in a sealed container at -0.3°C.

The samples were received in a laminar flow clean hood, void of trace metals contamination 

and ultra-violet radiation, and were designated discrete sample identifiers. An aliquot of 

each sample was filtered (0.45μm) and each filtrate was stored in a secure, monitored 

cryofreezer (maintained at a temperature of -80oC) until selenium speciation analysis could 

be performed via ion chromatography inductively coupled plasma collision reaction cell 

mass spectrometry (IC-ICP-CRC-MS). 

2. Sample Preparation 

All sample preparation is performed in laminar flow clean hoods known to be free from trace 

metals contamination. All applied water for dilutions and sample preservatives are 

monitored for contamination to account for any biases associated with the sample results. 

Selenium Speciation Analysis by IC-ICP-CRC-MS Prior to analysis, an aliquot of each 

sample was filtered with a syringe filter (0.45μm) and injected directly into a sealed 

autosampler vial. No further sample preparation was performed as any chemical alteration of 

a sample may shift the equilibrium of the system, resulting in changes in speciation ratios. 

3. Sample Analysis 

All sample analysis is preceded by a minimum of a five-point calibration curve spanning the 

entire concentration range of interest. Calibration curves are performed at the beginning of 

each analytical day. All calibration curves, associated with each species of interest, are 

Page 12 of 40
standardized by linear regression resulting in a response factor. All sample results are 

instrument blank corrected to account for any operational biases associated with the 

analytical platform. 

Prior to sample analysis, all calibration curves are verified using second source standards 

which are identified as initial calibration verification standards (ICV). 

Ongoing instrument performance is identified by the analysis of continuing calibration 

verification standards (CCV) and continuing calibration blanks (CCB) at a minimal interval 

of every ten analytical runs. 

Selenium Speciation Analysis by IC-ICP-CRC-MS Each sample for selenium speciation 

analysis was analyzed by ion chromatography inductively coupled plasma collision reaction 

cell mass spectrometry (IC-ICP-CRC-MS) on August 8, 2012. An aliquot of each sample is

injected onto an anion exchange column and mobilized by a basic (pH > 7) gradient. The 

eluting selenium species are then introduced into a radio frequency (RF) plasma where 

energy-transfer processes cause desolvation, atomization, and ionization. The ions are 

extracted from the plasma through a differentially-pumped vacuum interface and travel 

through a pressurized chamber (CRC) containing a reaction gas which preferentially reacts 

with interfering ions of the same target mass to charge ratios (m/z). A solid-state detector 

detects ions transmitted through the mass analyzer and the resulting current is processed by a 

data handling system. 

Retention times for each eluting species are compared to known standards for species 

identification. 

4. Analytical Issues 

The overall analyses went well and no significant analytical issues were encountered. All 

quality control parameters associated with the samples were within acceptance limits.

The estimated method detection limits (eMDLs) for selenite, selenate, and selenocyanate are 

generated from replicate analyses of the lowest standard in the calibration curve. Not all 

selenium species are present in preparation blanks; therefore, eMDL calculations based on 

preparation blanks are artificially biased low. 

The eMDL for methylseleninic acid and selenomethionine is calculated from the average 

eMDL of selenite, selenate, and selenocyanate. The calibration does not contain 

methylseleninic acid or selenomethionine due to impurities in these standards which would 

bias the results for other selenium species. 

Page 13 of 40
If you have any questions or concerns regarding this report, please feel free to contact me. 

Sincerely, 

Russell Gerads 

Vice President 

Applied Speciation and Consulting, LLC 

Page 14 of 40
Sample Results

Sample ID Se(IV) Se(VI) SeCN MeSe(IV) SeMe

Unknown Se 

Species (n)

FGD Purge Eff ND (<2.0) 573 ND (<1.5) ND (<2.1) ND (<2.1) 0.0 (0)

BioReactor 1 Inf 1.48 511 ND (<0.38) ND (<0.52) ND (<0.52) 0.0 (0)

BioReactor 2 Inf 19.7 1.86 0.46 ND (<0.52) ND (<0.52) 0.0 (0)

BioReactor 2 Eff 0.66 ND (<0.67) ND (<0.38) ND (<0.52) ND (<0.52) 0.0 (0)

All results reflect the applied dilution and are reported in μg/L

ND = Not detected at the applied dilution

SeCN = Selenocyanate

MeSe(IV) = Methylseleninic acid

SeMe = Selenomethionine

Unknown Se Species = Total concentration of all unknown Se species observed by IC-ICP-MS

n = number of unknown Se species observed

Report Generated by: Russell Gerads

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Allen Shay/MillerCreek

Contact: Jay Perkins

Date: August 16, 2012

LIMS #J12080128

Page 15 of 40
Quality Control Summary - Preparation Blank Summary

Analyte (μg/L) PBW1 PBW2 PBW3 PBW4 Mean StdDev eMDL* eMDL 250x eMDL 1000x

Se(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.0020 0.51 2.0

Se(VI) 0.000 0.000 0.000 0.000 0.000 0.000 0.0027 0.67 2.7

SeCN 0.000 0.000 0.000 0.000 0.000 0.000 0.0015 0.38 1.5

MeSe(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.0021 0.52 2.1

SeMe 0.000 0.000 0.000 0.000 0.000 0.000 0.0021 0.52 2.1

eMDL = Estimated Method Detection Limit

*Please see narrative regarding eMDL calculations

Quality Control Summary - Certified Reference Materials

Analyte (μg/L) CRM True Value Result Recovery

Se(IV) LCS 9.57 10.11 105.6

Se(VI) LCS 9.48 10.50 110.7

SeCN LCS 8.92 9.50 106.6

MeSe(IV) LCS 6.47 6.85 105.9

SeMe LCS 9.32 9.22 98.9

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Allen Shay/MillerCreek

Contact: Jay Perkins

LIMS #J12080128

Date: August 16, 2012

Report Generated by: Russell Gerads

Page 16 of 40
Quality Control Summary - Matrix Duplicates

Analyte (μg/L) Sample ID Rep 1 Rep 2 Mean RPD

Se(IV) BioReactor 1 Inf 1.48 1.57 1.52 6.3

Se(VI) BioReactor 1 Inf 511.4 511.6 511.5 0.0

SeCN BioReactor 1 Inf ND (<0.38) ND (<0.38) NC NC

MeSe(IV) BioReactor 1 Inf 0.45 0.44 0.44 2.2

SeMe BioReactor 1 Inf ND (<0.52) ND (<0.52) NC NC

ND = Not detected at the applied dilution

NC = Value was not calculated due to one or more concentrations below the eMDL

Quality Control Summary - Matrix Spike/ Matrix Spike Duplicate

Analyte (μg/L) Sample ID Spike Conc MS Result Recovery Spike Conc MSD Result Recovery RPD

Se(IV) BioReactor 1 Inf 1390 1336 96.0 1390 1312 94.3 1.8

Se(VI) BioReactor 1 Inf 1261 1717 95.6 1261 1694 93.7 1.4

SeCN BioReactor 1 Inf 1144 1028 89.8 1144 1007 88.0 2.0

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Allen Shay/MillerCreek

Contact: Jay Perkins

LIMS #J12080128

Date: August 16, 2012

Report Generated by: Russell Gerads

Page 17 of 40
Page 18 of 40
3958 6th Ave NW • Seattle, WA 98107 • T: 206-632-6206 • F: 206-632-6017 • www.brooksrand.com • brl@brooksrand.com

August 22, 2012

Duke Energy

ATTN: Jay Perkins

Scientific Support-Laboratory

13339 Hagers Ferry Road

Huntersville NC 28078

jcperkins@duke-energy.com

labcustomer@duke-energy.com

RE: Project DUK-HV1201 Client Project: J12080128

Dear Mr. Perkins,

On August 8, 2012, Brooks Rand Labs (BRL) received three (3) wastewater samples and three

(3) corresponding field blanks. An aliquot was removed from each sample bottle and filtered into 

a separate container designed for dissolved mercury (Hg) analysis. The sample volume from 

the original container was logged-in for total Hg analysis. All samples were received, prepared, 

analyzed, and stored according to BRL SOPs and EPA methodology.

Data used for regulatory purposes has a 24 hour filtration holding time requirement. Nonregulatory purposed data has a 48 hour filtration holding time. The samples were received

within the 48 hour filtration requirement.

The results were blank-corrected as described in the calculations section of the relevant SOP(s) 

and may have been evaluated using reporting limits that have been adjusted to account for 

sample aliquot size. Please refer to the Sample Results page for sample-specific MDLs, MRLs, 

and other details. 

Instrument blank –IBL1 was analyzed on a faulty purge vessel. The sample was then reanalyzed on good purge vessel, produced an acceptable result, and reported as –IBL5.

The original analyses of the low and high calibration standards were analyzed on the same 

faulty purge vessel as –IBL1. Both samples were re-analyzed and reported as –CAL6 and –

CAL7. The original purge vessel was removed from service.

The analysis of the first continuing calibration blank (-CCB1) was elevated. This was likely 

attributed to carry-over contamination from the analysis of –CAL7 (10,000 pg of Hg). The purge 

vessel used for the analysis of –CCB1 and –CAL7 was checked and reported as –CCB2, which 

yielded a very low result. All data was reported without qualification, aside from concentration 

qualifiers, and all associated quality control sample results met the acceptance criteria.

BRL, an accredited laboratory, certifies the reported results of all analyses for which BRL is 

NELAP accredited meet all NELAP requirements. For more details, see the Report Information

page of the report.

Page 19 of 40
Please feel free to contact us if you have any questions regarding this report.

Sincerely,

 

Tiffany Stilwater Mi Sun Um

Project Manager Data Manager

tiffany@brooksrand.com misun@brooksrand.com

Page 20 of 40
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Definition of Data Qualifiers

(Effective 9/23/09)

Laboratory Accreditation

BRL is accredited by the National Environmental Laboratory Accreditation Program (NELAP) through the State of Florida 

Department of Health, Bureau of Laboratories (E87982) and is certified to perform many environmental analyses. BRL is 

also certified by many other states to perform environmental analyses. For a current list of our accreditations /certifications, 

please visit our website at <http://www.brooksrand.com/default.asp?contentID=586>. Results reported relate only to the 

samples listed in the report.

Report Information

BLK

BRL

BS

CAL

CCV

CRM

D

DUP

ICV

MSD

ND

NR

PS

REC

RPD

RSD

SCV

SOP

method blank 

Brooks Rand Labs

laboratory fortified blank

calibration standard

continuing calibration verification

certified reference material

dissolved fraction

duplicate

initial calibration verification

matrix spike duplicate

non-detect

non-reportable

post preparation spike

percent recovery

relative percent difference

relative standard deviation

secondary calibration verification

standard operating procedure

MDL

MRL

MS

method detection limit

method reporting limit

matrix spike

SRM

T

COC

standard reference material

total recoverable fraction

chain of custody record 

Common Abbreviations

These qualifiers are based on those previously utilized by Brooks Rand Labs, those found in the EPA SOW ILM03.0, 

Exhibit B, Section III, pg. B-18, and the USEPA Contract Laboratory Program National Functional Guidelines for Inorganic 

Superfund Data Review; USEPA; January 2010. These supersede all previous qualifiers ever employed by BRL.

Detected by the instrument, the result is > the MDL but ≤ the MRL. Result is reported and considered an estimate.

B

E 

An estimated value due to the presence of interferences. A full explanation is presented in the narrative.

H 

Holding time and/or preservation requirements not met. Result is estimated.

J 

Estimated value. A full explanation is presented in the narrative.

J-M

Duplicate precision (RPD) for associated QC sample was not within acceptance criteria. Result is estimated.

J-N

Spike recovery for associated QC sample was not within acceptance criteria. Result is estimated.

M 

Duplicate precision (RPD) was not within acceptance criteria. Result is estimated.

N 

Spike recovery was not within acceptance criteria. Result is estimated.

R 

Rejected, unusable value. A full explanation is presented in the narrative.

U 

Result is ≤ the MDL or client requested reporting limit (CRRL). Result reported as the MDL or CRRL.

X 

Result is not BLK-corrected and is within 10x the absolute value of the highest detectable BLK in the batch. 

Result is estimated.

Field Quality Control Samples

Please be notified that certain EPA methods require the collection of field quality control samples of an appropriate type 

and frequency; failure to do so is considered a deviation from some methods and for compliance purposes should only be 

done with the approval of regulatory authorities. Please see the specific EPA methods for details regarding required field 

quality control samples.

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 21 of 40
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Information

Sample Lab ID Report Matrix Type Sampled Received

BioReactor 1 Inf 1232020-01 Influent Sample 08/06/2012 08/08/2012

BioReactor 1 Inf 1232020-02 Influent Sample 08/06/2012 08/08/2012

BioReactor 1 Inf Hg Blk 1232020-03 DIW Field Blank 08/06/2012 08/08/2012

BioReactor 1 Inf Hg Blk 1232020-04 DIW Field Blank 08/06/2012 08/08/2012

BioReactor 2 Inf 1232020-05 Influent Sample 08/06/2012 08/08/2012

BioReactor 2 Inf 1232020-06 Influent Sample 08/06/2012 08/08/2012

BioReactor 2 Inf Hg Blk 1232020-07 DIW Field Blank 08/06/2012 08/08/2012

BioReactor 2 Inf Hg Blk 1232020-08 DIW Field Blank 08/06/2012 08/08/2012

BioReactor 2 Eff 1232020-09 Effluent Sample 08/06/2012 08/08/2012

BioReactor 2 Eff 1232020-10 Effluent Sample 08/06/2012 08/08/2012

BioReactor 2 Eff Hg Blk 1232020-11 DIW Field Blank 08/06/2012 08/08/2012

BioReactor 2 Eff Hg Blk 1232020-12 DIW Field Blank 08/06/2012 08/08/2012

Batch Summary

Analyte Lab Matrix Method Prepared Analyzed Batch Sequence

Hg Water EPA 1631 08/10/2012 08/13/2012 B121425 1200625

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 22 of 40
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Results

Sample Analyte Report Matrix Basis Result Qualifier MDL MRL Unit Batch Sequence

BioReactor 1 Inf

1232020-01 Hg Influent T 6930 50.5 135 ng/L B121425 1200625

1232020-02 Hg Influent D 4480 30.3 80.8 ng/L B121425 1200625

BioReactor 1 Inf Hg Blk

1232020-03 Hg DIW T 0.15 U 0.15 0.41 ng/L B121425 1200625

1232020-04 Hg DIW D 0.15 U 0.15 0.40 ng/L B121425 1200625

BioReactor 2 Eff

1232020-09 Hg Effluent T 118 1.52 4.04 ng/L B121425 1200625

1232020-10 Hg Effluent D 7.32 0.15 0.40 ng/L B121425 1200625

BioReactor 2 Eff Hg Blk

1232020-11 Hg DIW T 0.15 U 0.15 0.40 ng/L B121425 1200625

1232020-12 Hg DIW D 0.15 U 0.15 0.40 ng/L B121425 1200625

BioReactor 2 Inf

1232020-05 Hg Influent T 1550 3.06 8.16 ng/L B121425 1200625

1232020-06 Hg Influent D 5.69 0.15 0.41 ng/L B121425 1200625

BioReactor 2 Inf Hg Blk

1232020-07 Hg DIW T 0.15 U 0.15 0.40 ng/L B121425 1200625

1232020-08 Hg DIW D 0.15 U 0.15 0.40 ng/L B121425 1200625

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 23 of 40
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Accuracy & Precision Summary

Batch: B121425

Analyte Native Spike Result Units REC & Limits RPD & Limits

Method: EPA 1631

Lab Matrix: Water

Sample

B121425-SRM1 Certified Reference Material (1229081, NIST 1641d 1000x dilution)

Hg 15.68 14.81 ng/L 94% 85-115

B121425-MS6 Matrix Spike (1232019-01)

Hg 18140 52630 65640 ng/L 90% 71-125

B121425-MSD6 Matrix Spike Duplicate (1232019-01)

Hg 18140 52630 68670 ng/L 96% 71-125 245%

B121425-MS5 Matrix Spike (1232019-09)

Hg 54.08 252.5 335.3 ng/L 111% 71-125

B121425-MSD5 Matrix Spike Duplicate (1232019-09)

Hg 54.08 252.5 295.7 ng/L 96% 71-125 2413%

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 24 of 40
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Method Blanks & Reporting Limits

Batch: B121425

Method: EPA 1631

Matrix: Water

Analyte: Hg

Sample Result Units

B121425-BLK1 0.10 ng/L

B121425-BLK2 0.17 ng/L

B121425-BLK3 0.09 ng/L

B121425-BLK4 0.06 ng/L

Average: 0.11 Standard Deviation: 0.05 MDL: 0.15

Limit: 0.50 Limit: 0.10 MRL: 0.40

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 25 of 40
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Instrument Calibration

Sequence: 1200625

Instrument: THG-05 Method: EPA 1631

Date: 08/13/2012

Total Mercury and Mercury Speciation by CVAFS

Analyte: Hg

Lab ID True Value Result Units REC & Limits

1200625-IBL2 5.17 pg of Hg

1200625-IBL3 4.59 pg of Hg

1200625-IBL4 7.11 pg of Hg

1200625-CAL2 100.0 99.63 pg of Hg 100%

1200625-CAL3 500.0 522.9 pg of Hg 105%

1200625-CAL4 2500 2631 pg of Hg 105%

1200625-ICV1 1568 1481 pg of Hg 94% 85-115

1200625-CCV1 500.0 490.6 pg of Hg 98% 77-123

1200625-IBL5 8.15 pg of Hg

1200625-CAL6 25.00 23.33 pg of Hg 93%

1200625-CAL7 10000 9821 pg of Hg 98%

1200625-CCB1 39.3 pg of Hg

1200625-CCB2 -0.94 pg of Hg

1200625-CCV2 500.0 411.4 pg of Hg 82% 77-123

1200625-CCV3 500.0 478.9 pg of Hg 96% 77-123

1200625-CCV4 500.0 395.3 pg of Hg 79% 77-123

1200625-CCV5 500.0 450.8 pg of Hg 90% 77-123

1200625-CCV6 500.0 392.1 pg of Hg 78% 77-123

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 26 of 40
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Containers

Lab ID: 1232020-01 Report Matrix: Influent

Sample: BioReactor 1 Inf Sample Type: Sample Received: 08/08/2012

Collected: 08/06/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71666330

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1232020-02 Report Matrix: Influent

Sample: BioReactor 1 Inf Sample Type: Sample Received: 08/08/2012

Collected: 08/06/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 250 mL none n/a Cooler

Comments: Split from THg Container

Lab ID: 1232020-03 Report Matrix: DIW

Sample: BioReactor 1 Inf Hg Blk Sample Type: Field Blank Received: 08/08/2012

Collected: 08/06/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71666330

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1232020-04 Report Matrix: DIW

Sample: BioReactor 1 Inf Hg Blk Sample Type: Field Blank Received: 08/08/2012

Collected: 08/06/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 250 mL none n/a Cooler

Comments: Split from THg Container

Lab ID: 1232020-05 Report Matrix: Influent

Sample: BioReactor 2 Inf Sample Type: Sample Received: 08/08/2012

Collected: 08/06/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71666330

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1232020-06 Report Matrix: Influent

Sample: BioReactor 2 Inf Sample Type: Sample Received: 08/08/2012

Collected: 08/06/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 250 mL none n/a Cooler

Comments: Split from THg Container

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 27 of 40
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Containers

Lab ID: 1232020-07 Report Matrix: DIW

Sample: BioReactor 2 Inf Hg Blk Sample Type: Field Blank Received: 08/08/2012

Collected: 08/06/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71666330

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1232020-08 Report Matrix: DIW

Sample: BioReactor 2 Inf Hg Blk Sample Type: Field Blank Received: 08/08/2012

Collected: 08/06/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 250 mL none n/a Cooler

Comments: Split from THg Container

Lab ID: 1232020-09 Report Matrix: Effluent

Sample: BioReactor 2 Eff Sample Type: Sample Received: 08/08/2012

Collected: 08/06/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71666330

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1232020-10 Report Matrix: Effluent

Sample: BioReactor 2 Eff Sample Type: Sample Received: 08/08/2012

Collected: 08/06/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 250 mL none n/a Cooler

Comments: Split from THg Container

Lab ID: 1232020-11 Report Matrix: DIW

Sample: BioReactor 2 Eff Hg Blk Sample Type: Field Blank Received: 08/08/2012

Collected: 08/06/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71666330

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1232020-12 Report Matrix: DIW

Sample: BioReactor 2 Eff Hg Blk Sample Type: Field Blank Received: 08/08/2012

Collected: 08/06/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 250 mL none n/a Cooler

Comments: Split from THg Container

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 28 of 40
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Shipping Containers

Cooler

Tracking No: 5353 0519 2804 via FedEx

Temperature: -0.8 °C

Coolant Type: Ice

Description: Cooler

Damaged in transit? No

Returned to client? No

Custody seals present? No

Custody seals intact? No

COC present? Yes

Received: August 8, 2012 9:15

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 29 of 40
Page 30 of 40
This data package contains the analytical results for the project identified above and includes a Case Narrative, Sample 

Results and Chain of Custody. Unless otherwise noted, all samples were received in acceptable condition and processed 

according to the referenced methods. 

Data qualifiers are flagged individually on each sample. A key reference for the data qualifiers appears at the end of this case 

narrative.

NC Certification No. 402

SC Certification No. 99012

NC Drinking Water Cert No. 37735

Case Narrative

08/13/2012

Duke Energy Corporation (04)

Jay Perkins

13339 Hagers Ferry Road

Huntersville, NC 28078

Project: Allen Shay/Miller Creek

Project No.: J12080128

Lab Submittal Date: 08/07/2012

Prism Work Order: 2080159

VA Certification No. 1287

Please call if you have any questions relating to this analytical report.

Respectfully,

PRISM LABORATORIES, INC.

VP Laboratory Services Reviewed By

Data Qualifiers Key Reference:

HT Sample received and analyzed outside of the hold time.

MDL Method Detection Limit

BRL Below Reporting Limit

RPD Relative Percent Difference

*

Results reported to the reporting limit. All other results are reported to the MDL with values between MDL and 

reporting limit indicated with a J.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 1 of 9

Page 31 of 40
Sample Receipt Summary

08/13/2012

Prism Work Order: 2080159

Client Sample ID Lab Sample ID Matrix Date Sampled Date Received

2012017083/FGD Purge Eff 2080159-01 Water 08/06/12 08/07/12

2012017085/BioReactor 1 Inf 2080159-02 Water 08/06/12 08/07/12

2012017087/BioReactor 2 Inf 2080159-03 Water 08/06/12 08/07/12

2012017089/BioReactor 2 Eff 2080159-04 Water 08/06/12 08/07/12

Samples received in good condition at 4.5 degrees C unless otherwise noted.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 2 of 9

Page 32 of 40
Laboratory Report

08/13/2012

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation (04) Project: Allen Shay/Miller Creek

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 2080159-01

Prism Work Order: 2080159

Time Collected: 08/06/12 08:03

Time Submitted: 08/07/12 16:25

2012017083/FGD Purge Eff

J12080128

General Chemistry Parameters

pH 7.2 HT pH Units 1 *SM4500-H B 8/8/12 11:30 JAB P2H0162

Total Alkalinity 74 mg/L 5.0 0.66 1 *SM2320 B 8/8/12 11:00 JAB P2H0159

Carbonate Alkalinity BRL mg/L 5.0 0.66 1 *SM2320 B 8/8/12 11:00 JAB P2H0160

Bicarbonate Alkalinity 74 mg/L 5.0 0.66 1 *SM2320 B 8/8/12 11:00 JAB P2H0161

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 3 of 9

Page 33 of 40
Laboratory Report

08/13/2012

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation (04) Project: Allen Shay/Miller Creek

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 2080159-02

Prism Work Order: 2080159

Time Collected: 08/06/12 08:11

Time Submitted: 08/07/12 16:25

2012017085/BioReactor 1 Inf

J12080128

General Chemistry Parameters

pH 7.3 HT pH Units 1 *SM4500-H B 8/8/12 11:30 JAB P2H0162

Total Alkalinity 79 mg/L 5.0 0.66 1 *SM2320 B 8/8/12 11:00 JAB P2H0159

Carbonate Alkalinity BRL mg/L 5.0 0.66 1 *SM2320 B 8/8/12 11:00 JAB P2H0160

Bicarbonate Alkalinity 79 mg/L 5.0 0.66 1 *SM2320 B 8/8/12 11:00 JAB P2H0161

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 4 of 9

Page 34 of 40
Laboratory Report

08/13/2012

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation (04) Project: Allen Shay/Miller Creek

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 2080159-03

Prism Work Order: 2080159

Time Collected: 08/06/12 08:20

Time Submitted: 08/07/12 16:25

2012017087/BioReactor 2 Inf

J12080128

General Chemistry Parameters

pH 7.0 HT pH Units 1 *SM4500-H B 8/8/12 11:30 JAB P2H0162

Total Alkalinity 280 mg/L 5.0 0.66 1 *SM2320 B 8/8/12 11:00 JAB P2H0159

Carbonate Alkalinity BRL mg/L 5.0 0.66 1 *SM2320 B 8/8/12 11:00 JAB P2H0160

Bicarbonate Alkalinity 280 mg/L 5.0 0.66 1 *SM2320 B 8/8/12 11:00 JAB P2H0161

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 5 of 9

Page 35 of 40
Laboratory Report

08/13/2012

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation (04) Project: Allen Shay/Miller Creek

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 2080159-04

Prism Work Order: 2080159

Time Collected: 08/06/12 08:15

Time Submitted: 08/07/12 16:25

2012017089/BioReactor 2 Eff

J12080128

General Chemistry Parameters

pH 7.4 HT pH Units 1 *SM4500-H B 8/8/12 11:30 JAB P2H0162

Total Alkalinity 290 mg/L 5.0 0.66 1 *SM2320 B 8/8/12 11:00 JAB P2H0159

Carbonate Alkalinity BRL mg/L 5.0 0.66 1 *SM2320 B 8/8/12 11:00 JAB P2H0160

Bicarbonate Alkalinity 290 mg/L 5.0 0.66 1 *SM2320 B 8/8/12 11:00 JAB P2H0161

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 6 of 9

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8/13/12

Level II QC Report

13339 Hagers Ferry Road Project No: J12080128

Duke Energy Corporation (04) Project: Allen Shay/Miller Creek

Huntersville, NC 28078

Attn: Jay Perkins

Prism Work Order: 2080159

Time Submitted: 8/7/2012 4:25:00PM

Result Limit

Reporting

Units Level

Spike

Result

Source

%REC

%REC

Limits RPD

RPD

 Analyte Limit Notes 

General Chemistry Parameters - Quality Control

Batch P2H0159 - NO PREP

Blank (P2H0159-BLK1) Prepared & Analyzed: 08/08/12 

Total Alkalinity BRL 5.0 mg/L

LCS (P2H0159-BS1) Prepared & Analyzed: 08/08/12 

Total Alkalinity 245 5.0 mg/L 250.0 98 90-110

LCS Dup (P2H0159-BSD1) Prepared & Analyzed: 08/08/12 

Total Alkalinity 245 5.0 mg/L 250.0 98 90-110 0 200

Duplicate (P2H0159-DUP1) Source: 2080159-04 Prepared & Analyzed: 08/08/12 

Total Alkalinity 307 5.0 mg/L 289 6 20

Batch P2H0160 - NO PREP

Blank (P2H0160-BLK1) Prepared & Analyzed: 08/08/12 

Carbonate Alkalinity BRL 5.0 mg/L

LCS (P2H0160-BS1) Prepared & Analyzed: 08/08/12 

Carbonate Alkalinity 245 5.0 mg/L 90-110

LCS Dup (P2H0160-BSD1) Prepared & Analyzed: 08/08/12 

Carbonate Alkalinity 245 5.0 mg/L 90-110 0 200

Duplicate (P2H0160-DUP1) Source: 2080159-04 Prepared & Analyzed: 08/08/12 

Carbonate Alkalinity BRL 5.0 mg/L BRL 20

Batch P2H0161 - NO PREP

Blank (P2H0161-BLK1) Prepared & Analyzed: 08/08/12 

Bicarbonate Alkalinity BRL 5.0 mg/L

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 7 of 9

Page 37 of 40
8/13/12

Level II QC Report

13339 Hagers Ferry Road Project No: J12080128

Duke Energy Corporation (04) Project: Allen Shay/Miller Creek

Huntersville, NC 28078

Attn: Jay Perkins

Prism Work Order: 2080159

Time Submitted: 8/7/2012 4:25:00PM

Result Limit

Reporting

Units Level

Spike

Result

Source

%REC

%REC

Limits RPD

RPD

 Analyte Limit Notes 

General Chemistry Parameters - Quality Control

Batch P2H0161 - NO PREP

LCS (P2H0161-BS1) Prepared & Analyzed: 08/08/12 

Bicarbonate Alkalinity 245 5.0 mg/L 250.0 98 90-110

LCS Dup (P2H0161-BSD1) Prepared & Analyzed: 08/08/12 

Bicarbonate Alkalinity 245 5.0 mg/L 250.0 98 90-110 0 200

Duplicate (P2H0161-DUP1) Source: 2080159-04 Prepared & Analyzed: 08/08/12 

Bicarbonate Alkalinity 307 5.0 mg/L 289 6 20

Batch P2H0162 - NO PREP

LCS (P2H0162-BS1) Prepared & Analyzed: 08/08/12 

pH 6.91 pH Units 6.860 101 99-101

Duplicate (P2H0162-DUP1) Source: 2080159-04 Prepared & Analyzed: 08/08/12 

pH 7.37 pH Units 7.36 0.1 10

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 8 of 9

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