### 13339 Hagers Ferry Road Huntersville, NC 28078-7929 McGuire Nuclear Complex - MG03A2 Phone: 980-875-5245 Fax: 980-875-4349 

# Order Summary Report 

# Analytical Laboratory

Order Number: J12080128 Project Name: Somerville Waste Water

L ab Contact: Jason C Perkins 

### Date: 8/24/2012 Customer Address: 253 Plant Allen Road Customer Name(s): BillK-RonL--RobnJ-DonS-RayL

Belmont, NC 28012

Phone: 980-875-5348 Report Authorized By: (Signature)


### Program Comments: Please contact the Program Manager (Jason C Perkins) with any questions regarding this report. Data Flags & Calculations: Any analytical tests or individual analytes within a test flagged with a Qualifier indicate a deviation from the method quality system or quality control requirement. The qualifier description is found at the end of the Certificate of Analysis (sample results) under the qualifiers heading. All results are reported on a dry weight basis unless otherwise noted. Subcontracted data included on the Duke Certificate of Analysis is to be used as information only. Certified vendor results can be found in the subcontracted lab final report. Duke Energy Analytical Laboratory subcontracts analyses to other vendor laboratories that have been qualified by Duke Energy to perform these analyses except where noted. Data Package: This data package includes analytical results that are applicable only to the samples described in this narrative. An estimation of the uncertainty of measurement for the results in the report is available upon request. This report shall not be reproduced, except in full, without the written consent of the Analytical Laboratory. Please contact the Analytical laboratory with any questions. The order of individual sections within this report is as follows: Job Summary Report, Sample Identification, Technical Validation of Data Package, Analytical Laboratory Certificate of Analysis, Analytical Laboratory QC Reports, Sub-contracted Laboratory Results, Customer Specific Data Sheets, Reports & Documentation, Customer Database Entries, Test Case Narratives, Chain of Custody (COC) Certification: The Analytical Laboratory holds the following State Certifications : North Carolina (DENR) Certificate #248, South Carolina (DHEC) Laboratory ID # 99005. Contact the Analytical Laboratory for definitive information about the certification status of specific methods.

## Sample ID's & Descriptions:

Sample ID Plant/Station Collection Date and Time Collected By Sample Description 2012017083 ALLEN 06-Aug-12 8:03 AM Jeff Womack FGD Purge Eff 2012017084 ALLEN 06-Aug-12 8:07 AM Jeff Womack EQ Tank Eff 2012017085 ALLEN 06-Aug-12 8:11 AM Jeff Womack BioReactor 1 Inf 2012017086 ALLEN 06-Aug-12 3:31 PM Jeff Womack BioReactor 1 Inf BLANK 2012017087 ALLEN 06-Aug-12 8:20 AM Jeff Womack BioReactor 2 Inf 2012017088 ALLEN 06-Aug-12 3:35 PM Jeff Womack BioReactor 2 Inf BLANK 2012017089 ALLEN 06-Aug-12 8:15 AM Jeff Womack BioReactor 2 Eff 2012017090 ALLEN 06-Aug-12 3:32 PM Jeff Womack BioReactor 2 Eff BLANK 2012017091 ALLEN 06-Aug-12 10:03 AM Jeff Womack Filter Blk 9 Total Samples

### COC and .pdf report are in agreement with sample totals

and analyses (compliance programs and procedures). All Results are less than the laboratory reporting limits. All laboratory QA/QC requirements are acceptable. Yes No



# Technical Validation Review Checklist:

Yes No
Yes No



## Report Sections Included:

Job Summary Report Sub-contracted Laboratory Results Sample Identification Customer Specific Data Sheets, Reports, & Documentation Technical Validation of Data Package Customer Database Entries Analytical Laboratory Certificate of Analysis Analytical Laboratory QC Report Chain of Custody

Reviewed By: 

### DataBase Administrator Date: 

### 8/24/2012 Electronic Data Deliverable (EDD) Sent Separately

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12080128

2012017083 Collection Date: 06-Aug-12 8:03 AM 

### Site: FGD Purge Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF ALKALINITY - (Analysis Performed by Prism Labs) Vendor Parameter Complete Vendor Method V_PRISM INORGANIC IONS BY IC Bromide 14 mg/L EPA 300.0 8/13/2012 5:25:00 PM JAHERMA 5 50 Chloride 2400 mg/L EPA 300.0 8/13/2012 5:25:00 PM JAHERMA 50 500 Sulfate 1600 mg/L EPA 300.0 8/13/2012 5:25:00 PM JAHERMA 50 500 MERCURY (COLD VAPOR) IN WATER Mercury (Hg) 31.2 ug/L EPA 245.1 8/16/2012 1:58:11 PM AGIBBS 2.5 50 DISSOLVED METALS BY ICP Manganese (Mn) < 0.05 mg/L EPA 200.7 8/13/2012 12:50:00 PM MHH7131 0.05 10 TOTAL RECOVERABLE METALS BY ICP Boron (B) 243 mg/L EPA 200.7 8/13/2012 11:01:00 AM MHH7131 0.5 10 Calcium (Ca) 1750 mg/L EPA 200.7 8/13/2012 11:01:00 AM MHH7131 0.1 10 Iron (Fe) 93.5 mg/L EPA 200.7 8/13/2012 11:01:00 AM MHH7131 0.1 10 Lithium (Li) 0.156 mg/L EPA 200.7 8/13/2012 11:01:00 AM MHH7131 0.05 10 Magnesium (Mg) 738 mg/L EPA 200.7 8/13/2012 11:01:00 AM MHH7131 0.05 10 Manganese (Mn) 3.26 mg/L EPA 200.7 8/13/2012 11:01:00 AM MHH7131 0.05 10 Potassium (K) 36.6 mg/L EPA 200.7 8/13/2012 11:01:00 AM MHH7131 1 10 Sodium (Na) 32.6 mg/L EPA 200.7 8/13/2012 11:01:00 AM MHH7131 0.5 10 DISSOLVED METALS BY ICP-MS Selenium (Se) 721 ug/L EPA 200.8 8/14/2012 1:39:00 PM DJSULL1 10 10 TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) 127 ug/L EPA 200.8 8/14/2012 12:23:00 PM DJSULL1 10 10 Cadmium (Cd) < 10 ug/L EPA 200.8 8/14/2012 12:23:00 PM DJSULL1 10 10 Chromium (Cr) 166 ug/L EPA 200.8 8/14/2012 12:23:00 PM DJSULL1 10 10 Copper (Cu) 119 ug/L EPA 200.8 8/14/2012 12:23:00 PM DJSULL1 10 10 Nickel (Ni) 202 ug/L EPA 200.8 8/14/2012 12:23:00 PM DJSULL1 10 10 Selenium (Se) 792 ug/L EPA 200.8 8/14/2012 12:23:00 PM DJSULL1 10 10 Silver (Ag) < 10 ug/L EPA 200.8 8/14/2012 12:23:00 PM DJSULL1 10 10 Zinc (Zn) 250 ug/L EPA 200.8 8/14/2012 12:23:00 PM DJSULL1 10 10 SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC) Vendor Parameter Complete Vendor Method V_AS&C TOTAL DISSOLVED SOLIDS TDS 10000 mg/L SM2540C 8/15/2012 4:29:00 PM TJA7067 200 1 TOTAL SUSPENDED SOLIDS TSS 2200 mg/L SM2540D 8/13/2012 2:26:00 PM TJA7067 250 1

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12080128

2012017083 Collection Date: 06-Aug-12 8:03 AM 

### Site: FGD Purge Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF


### 2012017084 Collection Date: 06-Aug-12 8:07 AM 

### Site: EQ Tank Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY (COLD VAPOR) IN WATER Mercury (Hg) 21.5 ug/L EPA 245.1 8/16/2012 2:00:35 PM AGIBBS 2.5 50 DISSOLVED METALS BY ICP Manganese (Mn) < 0.05 mg/L EPA 200.7 8/13/2012 12:54:00 PM MHH7131 0.05 10 TOTAL RECOVERABLE METALS BY ICP Boron (B) 255 mg/L EPA 200.7 8/13/2012 11:05:00 AM MHH7131 0.5 10 Calcium (Ca) 1900 mg/L EPA 200.7 8/13/2012 11:05:00 AM MHH7131 0.1 10 Iron (Fe) 65.6 mg/L EPA 200.7 8/13/2012 11:05:00 AM MHH7131 0.1 10 Lithium (Li) 0.143 mg/L EPA 200.7 8/13/2012 11:05:00 AM MHH7131 0.05 10 Magnesium (Mg) 721 mg/L EPA 200.7 8/13/2012 11:05:00 AM MHH7131 0.05 10 Manganese (Mn) 2.19 mg/L EPA 200.7 8/13/2012 11:05:00 AM MHH7131 0.05 10 Potassium (K) 34.9 mg/L EPA 200.7 8/13/2012 11:05:00 AM MHH7131 1 10 Sodium (Na) 37.0 mg/L EPA 200.7 8/13/2012 11:05:00 AM MHH7131 0.5 10 DISSOLVED METALS BY ICP-MS Selenium (Se) 610 ug/L EPA 200.8 8/14/2012 1:42:00 PM DJSULL1 10 10 TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) 87.9 ug/L EPA 200.8 8/14/2012 12:26:00 PM DJSULL1 10 10 Cadmium (Cd) < 10 ug/L EPA 200.8 8/14/2012 12:26:00 PM DJSULL1 10 10 Chromium (Cr) 123 ug/L EPA 200.8 8/14/2012 12:26:00 PM DJSULL1 10 10 Copper (Cu) 88.2 ug/L EPA 200.8 8/14/2012 12:26:00 PM DJSULL1 10 10 Nickel (Ni) 141 ug/L EPA 200.8 8/14/2012 12:26:00 PM DJSULL1 10 10 Selenium (Se) 779 ug/L EPA 200.8 8/14/2012 12:26:00 PM DJSULL1 10 10 Silver (Ag) < 10 ug/L EPA 200.8 8/14/2012 12:26:00 PM DJSULL1 10 10 Zinc (Zn) 172 ug/L EPA 200.8 8/14/2012 12:26:00 PM DJSULL1 10 10


### 2012017085 Collection Date: 06-Aug-12 8:11 AM 

### Site: BioReactor 1 Inf Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF ALKALINITY - (Analysis Performed by Prism Labs) Vendor Parameter Complete Vendor Method V_PRISM

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12080128

2012017085 Collection Date: 06-Aug-12 8:11 AM 

### Site: BioReactor 1 Inf Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND DISSOLVED METALS BY ICP Manganese (Mn) < 0.05 mg/L EPA 200.7 8/13/2012 12:58:00 PM MHH7131 0.05 10 TOTAL RECOVERABLE METALS BY ICP Boron (B) 256 mg/L EPA 200.7 8/13/2012 11:09:00 AM MHH7131 0.5 10 Calcium (Ca) 1800 mg/L EPA 200.7 8/13/2012 11:09:00 AM MHH7131 0.1 10 Iron (Fe) < 0.1 mg/L EPA 200.7 8/13/2012 11:09:00 AM MHH7131 0.1 10 Lithium (Li) 0.081 mg/L EPA 200.7 8/13/2012 11:09:00 AM MHH7131 0.05 10 Magnesium (Mg) 639 mg/L EPA 200.7 8/13/2012 11:09:00 AM MHH7131 0.05 10 Manganese (Mn) < 0.05 mg/L EPA 200.7 8/13/2012 11:09:00 AM MHH7131 0.05 10 Potassium (K) 19.8 mg/L EPA 200.7 8/13/2012 11:09:00 AM MHH7131 1 10 Sodium (Na) 39.4 mg/L EPA 200.7 8/13/2012 11:09:00 AM MHH7131 0.5 10 DISSOLVED METALS BY ICP-MS Selenium (Se) 624 ug/L EPA 200.8 8/14/2012 1:46:00 PM DJSULL1 10 10 TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) < 10 ug/L EPA 200.8 8/14/2012 12:29:00 PM DJSULL1 10 10 Cadmium (Cd) < 10 ug/L EPA 200.8 8/14/2012 12:29:00 PM DJSULL1 10 10 Chromium (Cr) 15.6 ug/L EPA 200.8 8/14/2012 12:29:00 PM DJSULL1 10 10 Copper (Cu) < 10 ug/L EPA 200.8 8/14/2012 12:29:00 PM DJSULL1 10 10 Nickel (Ni) < 10 ug/L EPA 200.8 8/14/2012 12:29:00 PM DJSULL1 10 10 Selenium (Se) 695 ug/L EPA 200.8 8/14/2012 12:29:00 PM DJSULL1 10 10 Silver (Ag) < 10 ug/L EPA 200.8 8/14/2012 12:29:00 PM DJSULL1 10 10 Zinc (Zn) < 10 ug/L EPA 200.8 8/14/2012 12:29:00 PM DJSULL1 10 10 SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC) Vendor Parameter Complete Vendor Method V_AS&C


### 2012017086 Collection Date: 06-Aug-12 3:31 PM 

### Site: BioReactor 1 Inf BLANK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12080128

2012017086 Collection Date: 06-Aug-12 3:31 PM 

### Site: BioReactor 1 Inf BLANK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND


### 2012017087 Collection Date: 06-Aug-12 8:20 AM 

### Site: BioReactor 2 Inf Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF ALKALINITY - (Analysis Performed by Prism Labs) Vendor Parameter Complete Vendor Method V_PRISM MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND DISSOLVED METALS BY ICP Manganese (Mn) < 0.05 mg/L EPA 200.7 8/13/2012 1:02:00 PM MHH7131 0.05 10 TOTAL RECOVERABLE METALS BY ICP Boron (B) 259 mg/L EPA 200.7 8/13/2012 11:13:00 AM MHH7131 0.5 10 Calcium (Ca) 1830 mg/L EPA 200.7 8/13/2012 11:13:00 AM MHH7131 0.1 10 Iron (Fe) 0.164 mg/L EPA 200.7 8/13/2012 11:13:00 AM MHH7131 0.1 10 Lithium (Li) 0.088 mg/L EPA 200.7 8/13/2012 11:13:00 AM MHH7131 0.05 10 Magnesium (Mg) 646 mg/L EPA 200.7 8/13/2012 11:13:00 AM MHH7131 0.05 10 Manganese (Mn) < 0.05 mg/L EPA 200.7 8/13/2012 11:13:00 AM MHH7131 0.05 10 Potassium (K) 33.2 mg/L EPA 200.7 8/13/2012 11:13:00 AM MHH7131 1 10 Sodium (Na) 39.5 mg/L EPA 200.7 8/13/2012 11:13:00 AM MHH7131 0.5 10 DISSOLVED METALS BY ICP-MS Selenium (Se) 41.3 ug/L EPA 200.8 8/14/2012 1:49:00 PM DJSULL1 10 10 TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) < 10 ug/L EPA 200.8 8/14/2012 12:32:00 PM DJSULL1 10 10 Cadmium (Cd) < 10 ug/L EPA 200.8 8/14/2012 12:32:00 PM DJSULL1 10 10 Chromium (Cr) < 10 ug/L EPA 200.8 8/14/2012 12:32:00 PM DJSULL1 10 10 Copper (Cu) < 10 ug/L EPA 200.8 8/14/2012 12:32:00 PM DJSULL1 10 10 Nickel (Ni) < 10 ug/L EPA 200.8 8/14/2012 12:32:00 PM DJSULL1 10 10 Selenium (Se) 44.9 ug/L EPA 200.8 8/14/2012 12:32:00 PM DJSULL1 10 10 Silver (Ag) < 10 ug/L EPA 200.8 8/14/2012 12:32:00 PM DJSULL1 10 10 Zinc (Zn) < 10 ug/L EPA 200.8 8/14/2012 12:32:00 PM DJSULL1 10 10

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12080128

2012017087 Collection Date: 06-Aug-12 8:20 AM 

### Site: BioReactor 2 Inf Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC) Vendor Parameter Complete Vendor Method V_AS&C


### 2012017088 Collection Date: 06-Aug-12 3:35 PM 

### Site: BioReactor 2 Inf BLANK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND


### 2012017089 Collection Date: 06-Aug-12 8:15 AM 

### Site: BioReactor 2 Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF ALKALINITY - (Analysis Performed by Prism Labs) Vendor Parameter Complete Vendor Method V_PRISM INORGANIC IONS BY IC Bromide 16 mg/L EPA 300.0 8/13/2012 4:32:00 PM JAHERMA 5 50 Chloride 3300 mg/L EPA 300.0 8/13/2012 4:32:00 PM JAHERMA 50 500 Sulfate 1800 mg/L EPA 300.0 8/13/2012 4:32:00 PM JAHERMA 50 500 MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND DISSOLVED METALS BY ICP Manganese (Mn) < 0.05 mg/L EPA 200.7 8/13/2012 1:06:00 PM MHH7131 0.05 10

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12080128

2012017089 Collection Date: 06-Aug-12 8:15 AM 

### Site: BioReactor 2 Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF TOTAL RECOVERABLE METALS BY ICP Boron (B) 257 mg/L EPA 200.7 8/13/2012 11:16:00 AM MHH7131 0.5 10 Calcium (Ca) 1820 mg/L EPA 200.7 8/13/2012 11:16:00 AM MHH7131 0.1 10 Iron (Fe) 0.266 mg/L EPA 200.7 8/13/2012 11:16:00 AM MHH7131 0.1 10 Lithium (Li) 0.092 mg/L EPA 200.7 8/13/2012 11:16:00 AM MHH7131 0.05 10 Magnesium (Mg) 653 mg/L EPA 200.7 8/13/2012 11:16:00 AM MHH7131 0.05 10 Manganese (Mn) < 0.05 mg/L EPA 200.7 8/13/2012 11:16:00 AM MHH7131 0.05 10 Potassium (K) 33.8 mg/L EPA 200.7 8/13/2012 11:16:00 AM MHH7131 1 10 Sodium (Na) 36.2 mg/L EPA 200.7 8/13/2012 11:16:00 AM MHH7131 0.5 10 DISSOLVED METALS BY ICP-MS Selenium (Se) 10.6 ug/L EPA 200.8 8/14/2012 1:52:00 PM DJSULL1 5 5 TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) < 5 ug/L EPA 200.8 8/14/2012 12:35:00 PM DJSULL1 5 5 Cadmium (Cd) < 5 ug/L EPA 200.8 8/14/2012 12:35:00 PM DJSULL1 5 5 Chromium (Cr) < 5 ug/L EPA 200.8 8/14/2012 12:35:00 PM DJSULL1 5 5 Copper (Cu) < 5 ug/L EPA 200.8 8/14/2012 12:35:00 PM DJSULL1 5 5 Nickel (Ni) < 5 ug/L EPA 200.8 8/14/2012 12:35:00 PM DJSULL1 5 5 Selenium (Se) 9.07 ug/L EPA 200.8 8/14/2012 12:35:00 PM B DJSULL1

5 5 Silver (Ag) < 5 ug/L EPA 200.8 8/14/2012 12:35:00 PM DJSULL1 5 5 Zinc (Zn) < 5 ug/L EPA 200.8 8/14/2012 12:35:00 PM DJSULL1 5 5 SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC) Vendor Parameter Complete Vendor Method V_AS&C


### 2012017090 Collection Date: 06-Aug-12 3:32 PM 

### Site: BioReactor 2 Eff BLANK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12080128

2012017091 Collection Date: 06-Aug-12 10:03 AM 

### Site: Filter Blk Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF DISSOLVED METALS BY ICP Manganese (Mn) < 0.005 mg/L EPA 200.7 8/13/2012 1:10:00 PM MHH7131 0.005 1 DISSOLVED METALS BY ICP-MS Selenium (Se) 1.21 ug/L EPA 200.8 8/14/2012 1:24:00 PM B DJSULL1

1 1



### Qualifiers: B Target analyte detected in method blank at or above the reporting limit. Target analyte concentration in sample is less than 10X the concentration in the method blank. Analyte concentration in sample could be due to contamination.

## August 1 6 , 201 2

Jay Perkins Duke Energy Analytical Laboratory Mail Code MGO3A2 (Building 7405) 13339 Hagers Ferry Rd. Huntersville, NC 28078 (704) 875-5 245 Project: Allen Shay/MillerCreek (LIMS #J12080128)

Dear Mr. Perkins, Attached is the report associated with four (4 ) aqueous samples submitted for selenium speciation analysis on August 7, 2012. The samples were received in a sealed cooler at-0.3 ° C on August 8, 2012

. Selenium speciation analysis was performed via ion chromatography inductively coupled plasma collision reaction cell mass spectrometry (IC-ICP- CRC -MS). Any issues associated with the analysis are addressed in the following report. If you have any questions, please feel free to contact me at your convenience.

Si ncerely,

Russell Gerads Vice President Applied Speciation and Consulting, LLC

## Applied Speciation and Consulting, LLC Report prepared for: Jay Perkins Duke Energy Analytical Laboratory Mail Code MGO3A2 (Building 7405) 13339 Hagers Ferry Rd. Huntersville, NC 28078 Project: Allen Shay/MillerCreek (LIMS #J12080128)

August 16 , 2012

1. Sample Reception Four (4 ) aqueous samples in125mL HDPE bottles (provided by Applied Speciation and Consulting) were submitted for selenium speciation analysis on August 7, 2012. The samples were received on August 8, 201 2 in a sealed container at -0.3 ° C. The samples were received in a laminar flow clean hood, void of trace metals contamination and ultra-violet radiation, and were designated discrete sample identifiers. An aliquot of each sample was filtered (0.45μm) and each filtrate w as stored in a secure, monitored cryofreezer (maintained at a temperature of -80 o 

## C) until selenium speciation analysis could be performed via ion chromatography inductively coupled plasma collision reaction cell mass spectrometry (IC-ICP- CRC -MS).

2. Sample Preparation All sample preparation is performed in laminar flow clean hoods known to be free from trace metals contamination. All applied water for dilutions and sample preservatives are monitored for contamination to account for any biases associated with the sample results. Selenium Speciation Analysis by IC-ICP-CRC-MS Prior to analysis, an aliquot of each sample was filtered with a syringe filter (0.45μm) and injected directly into a sealed autosampler vial. No further sample preparation was performed as any chemical alteration of a sample may shift the equilibrium of the system, resulting in changes in speciation ratios. 3. Sample Analysis All samp leanalysis is preceded by a minimum of a five-point calibration curve spanning the entire concentration range of interest. Calibration curves are performed at the beginning of each analytical day. All calibration curves, associated with each species of interest, are

## standardized by linear regression resulting in a response factor. All sample results are

instrument blank correctedto account for any operational biases associated with the analytical platform. Prior to sample analysis, all calibration curves are verified using second source standard s which are identified as initial calibration verification standards (ICV). Ongoing instrument performance is identified by the analysis of continuing calibration verification standards (CCV) and continuing calibration blanks (CCB) at a minimal interval of every ten analytical runs. Selenium Speciation Analysis by IC-ICP-CRC-MS Eachsample for selenium speciation analysis w asanalyzed by ion chromatography inductively coupled plasma collision reaction cell ma ss spectrometry (IC-ICP- CRC -MS) on August 8, 2012. An aliquot of eachsample is injected onto an anion exchange column and mobilized by a basic (pH > 7) gradient. The eluting selenium species are then introduced into a radio frequency (RF) plasma where energy-transfer processes cause desolvation, atomization, and ionization. The ions are extracted from the plasma through a differentially-pumped vacuum interface and travel through a pressurized chamber ( CRC ) containing a reaction gas which preferentially reacts with interfering ions of the same target mass to charge ratios (m/z). A solid-state detector detects ions transmitted through the mass analyzer and the resulting current is processed by a data handling system. Retention times for each eluting species are compared to known standards for species identification.

4. Analytical Issues The overall analyses went well and no significant analytical issues were encountered. All quality control parameters associated with the samples were within acceptance limits

. The estimated method detection limits (eMDLs) for selenite, selenate, and selenocyanate are generated from replicate analyses of the lowest standard in the calibration curve. Not all selenium species are present in preparation blanks; therefore, eMDL calculations based on preparation blanks are artificially biased low. The eMDL for methylseleninic acid and selenomethionine is calculated from the average eMDL of selenite, selenate, and selenocyanate. The calibration does not contain methylseleninic acid or selenomethionine due to impurities in these standards which would bias the results for other selenium species.

## If you have any questions or concerns regarding this report, please feel free to contact me.

Sincerely,

Russell Gerads Vice President Applied Speciation and Consulting, LLC

### Sample Results Sample ID Se(IV) Se(VI) SeCN MeSe(IV) SeMe Unknown Se Species (n) FGD Purge Eff ND (<2.0) 573 ND (<1.5) ND (<2.1) ND (<2.1) 0.0 (0) BioReactor 1 Inf 1.48 511 ND (<0.38) ND (<0.52) ND (<0.52) 0.0 (0) BioReactor 2 Inf 19.7 1.86 0.46 ND (<0.52) ND (<0.52) 0.0 (0) BioReactor 2 Eff 0.66 ND (<0.67) ND (<0.38) ND (<0.52) ND (<0.52) 0.0 (0) All results reflect the applied dilution and are reported in μg/L ND = Not detected at the applied dilution SeCN = Selenocyanate MeSe(IV) = Methylseleninic acid SeMe = Selenomethionine Unknown Se Species = Total concentration of all unknown Se species observed by IC-ICP-MS n = number of unknown Se species observed Report Generated by: Russell Gerads Applied Speciation and Consulting, LLC Selenium Speciation Results for Duke Energy Project Name: Allen Shay/MillerCreek Contact: Jay Perkins
Date: August 16, 2012 LIMS #J12080128

### Quality Control Summary - Preparation Blank Summary

Analyte (μg/L) PBW1 PBW2 PBW3 PBW4 Mean StdDev eMDL* eMDL 250x eMDL 1000x Se(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.0020 0.51 2.0 Se(VI) 0.000 0.000 0.000 0.000 0.000 0.000 0.0027 0.67 2.7 SeCN 0.000 0.000 0.000 0.000 0.000 0.000 0.0015 0.38 1.5 MeSe(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.0021 0.52 2.1 SeMe 0.000 0.000 0.000 0.000 0.000 0.000 0.0021 0.52 2.1 eMDL = Estimated Method Detection Limit *Please see narrative regarding eMDL calculations
Quality Control Summary - Certified Reference Materials Analyte (μg/L) CRM True Value Result Recovery Se(IV) LCS 9.57 10.11 105.6 Se(VI) LCS 9.48 10.50 110.7 SeCN LCS 8.92 9.50 106.6 MeSe(IV) LCS 6.47 6.85 105.9 SeMe LCS 9.32 9.22 98.9 Applied Speciation and Consulting, LLC Selenium Speciation Results for Duke Energy Project Name: Allen Shay/MillerCreek Contact: Jay Perkins LIMS #J12080128 Date: August 16, 2012 Report Generated by: Russell Gerads

### Quality Control Summary - Matrix Duplicates

Analyte (μg/L) Sample ID Rep 1 Rep 2 Mean RPD Se(IV) BioReactor 1 Inf 1.48 1.57 1.52 6.3 Se(VI) BioReactor 1 Inf 511.4 511.6 511.5 0.0 SeCN BioReactor 1 Inf ND (<0.38) ND (<0.38) NC NC MeSe(IV) BioReactor 1 Inf 0.45 0.44 0.44 2.2 SeMe BioReactor 1 Inf ND (<0.52) ND (<0.52) NC NC ND = Not detected at the applied dilution NC = Value was not calculated due to one or more concentrations below the eMDL

Quality Control Summary - Matrix Spike/ Matrix Spike Duplicate Analyte (μg/L) Sample ID Spike Conc MS Result Recovery Spike Conc MSD Result Recovery RPD Se(IV) BioReactor 1 Inf 1390 1336 96.0 1390 1312 94.3 1.8 Se(VI) BioReactor 1 Inf 1261 1717 95.6 1261 1694 93.7 1.4 SeCN BioReactor 1 Inf 1144 1028 89.8 1144 1007 88.0 2.0 Applied Speciation and Consulting, LLC Selenium Speciation Results for Duke Energy Project Name: Allen Shay/MillerCreek Contact: Jay Perkins LIMS #J12080128 Date: August 16, 2012 Report Generated by: Russell Gerads

### 3958 6 th 

### Ave NW • Seattle, WA 98107 • T: 206

- 632

- 6206 • F: 206

- 632

- 6017 • www.brooksrand.com • brl@brooksrand.com 

## August 22, 2012 Duke Energy ATTN : Jay Perkins Scientific Support
- Laboratory 13339 Hagers Ferry Road Huntersville NC 28078 jcperkins@duke

- energy.com labcustomer@duke

- energy.com
RE: Project DUK
- HV1 201 Client P roject : J12080128 Dear Mr. Perkins, O n August 8 , 2012, Brooks Rand Labs ( BRL ) received three(3 ) wastewater sample s and three (3) corresponding field blank s. An aliquot was removed from each sample bottle and filtered into a separate container designed for dissolved mercury (Hg) analysis. The sample volume from the original container was logged
- in for total Hg analysis

. All samples were received, prepared, analyzed, and stored according to BRL SOPs and EPA methodology. Data used for regulatory purposes has a 24 hour filtration holding time requirement. Non
regulatory purposed data has a 48 hour filtration holding time. The samples were received within the 48 hour filtration requirement. The results were blank
- corrected as described in the calculations section of the relevant SOP(s) and may have been evaluated using reporting limits that have been adjusted to account for sample aliquot size. Please refer to the Sample Results page for sample
- specific MDLs, MRLs, and other details. Instrument blank

– IBL1 was analyzed on a faulty purge vessel. The sample was then re
analyzed on good purge vessel, produced an acceptable result, and reported as

– IBL5. The original analyses of the low and high calibration standards were analyzed on the same faulty purge vessel as

– IBL1. Both samples were re
- analyzed and reported as

– CAL6 and

– CAL7. The original purge vessel was removed from service. The analysis of the first continuing calibration blank (
- CCB1) was elevated. This was likely attributed to carry
- over contamination from the analysis of

– CAL7 (10,000 pg of Hg). The purge vessel used for the analysis of

–CCB1 and

–CAL7 was checked and reported as

–CCB2, which yielded a very low result. All data wa s reported without qualification, aside from concentration qualifiers, and all associated q uality control sample results met the acceptance criteria. BRL, an accredited laboratory, certifies the reported results of all analyses for which BRL is NELAP accredited meet all NELAP requirements. For more details , see the Report Information page of the report.

## Please feel free to contact us if you have any questions regarding this report. Sincerely,

Tiffany Stilwater Mi Sun Um Project Manager Data Manager tiffany@brooksrand.com misun@brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



### Definition of Data Qualifiers

(Effective 9/23/09) 

### Laboratory Accreditation BRL is accredited by the National Environmental Laboratory Accreditation Program (NELAP) through the State of Florida Department of Health, Bureau of Laboratories (E87982) and is certified to perform many environmental analyses. BRL is also certified by many other states to perform environmental analyses. For a current list of our accreditations /certifications, please visit our website at <http://www.brooksrand.com/default.asp?contentID=586>. Results reported relate only to the samples listed in the report. 

# Report Information

BLK BRL BS CAL CCV CRM D DUP ICV MSD ND NR PS REC RPD RSD SCV SOP 

### method blank Brooks Rand Labs laboratory fortified blank calibration standard continuing calibration verification certified reference material dissolved fraction duplicate initial calibration verification matrix spike duplicate non-detect non-reportable post preparation spike percent recovery relative percent difference relative standard deviation secondary calibration verification standard operating procedure MDL MRL MS



### method detection limit method reporting limit matrix spike

SRM T COC



### standard reference material total recoverable fraction chain of custody record 

### Common Abbreviations

These qualifiers are based on those previously utilized by Brooks Rand Labs, those found in the EPA SOW ILM03.0, Exhibit B, Section III, pg. B-18, and the USEPA Contract Laboratory Program National Functional Guidelines for Inorganic Superfund Data Review; USEPA; January 2010. These supersede all previous qualifiers ever employed by BRL. Detected by the instrument, the result is > the MDL but ≤the MRL. Result is reported and considered an estimate. B E 

### An estimated value due to the presence of interferences. A full explanation is presented in the narrative. H 

### Holding time and/or preservation requirements not met. Result is estimated. J 

### Estimated value. A full explanation is presented in the narrative. J-M 

### Duplicate precision (RPD) for associated QC sample was not within acceptance criteria. Result is estimated. J-N 

### Spike recovery for associated QC sample was not within acceptance criteria. Result is estimated. M 

### Duplicate precision (RPD) was not within acceptance criteria. Result is estimated. N 

### Spike recovery was not within acceptance criteria. Result is estimated. R 

### Rejected, unusable value. A full explanation is presented in the narrative. U 

### Result is ≤the MDL or client requested reporting limit (CRRL). Result reported as the MDL or CRRL. X 

### Result is not BLK-corrected and is within 10x the absolute value of the highest detectable BLK in the batch. Result is estimated. 

### Field Quality Control Samples Please be notified that certain EPA methods require the collection of field quality control samples of an appropriate type and frequency; failure to do so is considered a deviation from some methods and for compliance purposes should only be done with the approval of regulatory authorities. Please see the specific EPA methods for details regarding required field quality control samples.

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Sample Information

Report Matrix Type Received Sampled Sample Lab ID 

### 1232020-01 BioReactor 1 Inf 08/06/2012 08/08/2012 Sample Influent 1232020-02 BioReactor 1 Inf 08/06/2012 08/08/2012 Sample Influent 1232020-03 BioReactor 1 Inf Hg Blk 08/06/2012 08/08/2012 Field Blank DIW 1232020-04 BioReactor 1 Inf Hg Blk 08/06/2012 08/08/2012 Field Blank DIW 1232020-05 BioReactor 2 Inf 08/06/2012 08/08/2012 Sample Influent 1232020-06 BioReactor 2 Inf 08/06/2012 08/08/2012 Sample Influent 1232020-07 BioReactor 2 Inf Hg Blk 08/06/2012 08/08/2012 Field Blank DIW 1232020-08 BioReactor 2 Inf Hg Blk 08/06/2012 08/08/2012 Field Blank DIW 1232020-09 BioReactor 2 Eff 08/06/2012 08/08/2012 Sample Effluent 1232020-10 BioReactor 2 Eff 08/06/2012 08/08/2012 Sample Effluent 1232020-11 BioReactor 2 Eff Hg Blk 08/06/2012 08/08/2012 Field Blank DIW 1232020-12 BioReactor 2 Eff Hg Blk 08/06/2012 08/08/2012 Field Blank DIW 

# Batch Summary

Analyte Prepared Analyzed Sequence Batch Lab Matrix Method 

### B121425 08/10/2012 08/13/2012 1200625 Water EPA 1631 Hg

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Sample Results

Sample Sequence Result MDL MRL Unit Batch

Qualifier Analyte Basis

Report Matrix BioReactor 1 Inf 

### 6930 T 1200625 B121425 Influent 135 50.5 1232020-01 Hg ng/L 4480 D 1200625 B121425 Influent 80.8 30.3 1232020-02 Hg ng/L
BioReactor 1 Inf Hg Blk 

### 0.15 T U 1200625 B121425 DIW 0.41 0.15 1232020-03 Hg ng/L 0.15 D U 1200625 B121425 DIW 0.40 0.15 1232020-04 Hg ng/L

BioReactor 2 Eff 

### 118 T 1200625 B121425 Effluent 4.04 1.52 1232020-09 Hg ng/L 7.32 D 1200625 B121425 Effluent 0.40 0.15 1232020-10 Hg ng/L
BioReactor 2 Eff Hg Blk 

### 0.15 T U 1200625 B121425 DIW 0.40 0.15 1232020-11 Hg ng/L 0.15 D U 1200625 B121425 DIW 0.40 0.15 1232020-12 Hg ng/L

BioReactor 2 Inf 

### 1550 T 1200625 B121425 Influent 8.16 3.06 1232020-05 Hg ng/L 5.69 D 1200625 B121425 Influent 0.41 0.15 1232020-06 Hg ng/L
BioReactor 2 Inf Hg Blk 

### 0.15 T U 1200625 B121425 DIW 0.40 0.15 1232020-07 Hg ng/L 0.15 D U 1200625 B121425 DIW 0.40 0.15 1232020-08 Hg ng/L

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Accuracy & Precision Summary

Batch: 

### B121425

Analyte Result Units Native Spike REC & Limits RPD & Limits Method: 

### EPA 1631 Lab Matrix: 

### Water

Sample Certified Reference Material (1229081, NIST 1641d 1000x dilution) B121425-SRM1 

### 15.68 94% ng/L 85-115 Hg 14.81
Matrix Spike (1232019-01) B121425-MS6 

### 52630 90% ng/L 71-125 Hg 65640

18140
Matrix Spike Duplicate (1232019-01) B121425-MSD6 

### 52630 96% ng/L 71-125 245% Hg 68670

18140
Matrix Spike (1232019-09) B121425-MS5 

### 252.5 111% ng/L 71-125 Hg 335.3

54.08
Matrix Spike Duplicate (1232019-09) B121425-MSD5 

### 252.5 96% ng/L 71-125 2413% Hg 295.7

54.08

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Method Blanks & Reporting Limits

Batch: 

### B121425
Method: 

### EPA 1631 Matrix: 

### Water Analyte: 

### Hg Result Units Sample 

### B121425-BLK1 ng/L 0.10 B121425-BLK2 ng/L 0.17 B121425-BLK3 ng/L 0.09 B121425-BLK4 ng/L 0.06 MDL: 

### 0.15 Average: 

### 0.11 Standard Deviation: 

### 0.05 Limit: 

### 0.10 Limit: 

### 0.50 MRL: 

### 0.40

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Instrument Calibration

Sequence: 

### 1200625 Method: 

### EPA 1631 Instrument: 

### THG-05 Date: 

### 08/13/2012 Total Mercury and Mercury Speciation by CVAFS
Analyte: 

### Hg REC & Limits Result True Value Lab ID Units 

### 1200625-IBL2 5.17 pg of Hg 1200625-IBL3 4.59 pg of Hg 1200625-IBL4 7.11 pg of Hg 1200625-CAL2 100.0 99.63 pg of Hg 100% 1200625-CAL3 500.0 522.9 pg of Hg 105% 1200625-CAL4 2500 2631 pg of Hg 105% 1200625-ICV1 1568 1481 pg of Hg 94% 85-115 1200625-CCV1 500.0 490.6 pg of Hg 98% 77-123 1200625-IBL5 8.15 pg of Hg 1200625-CAL6 25.00 23.33 pg of Hg 93% 1200625-CAL7 10000 9821 pg of Hg 98% 1200625-CCB1 39.3 pg of Hg 1200625-CCB2 -0.94 pg of Hg 1200625-CCV2 500.0 411.4 pg of Hg 82% 77-123 1200625-CCV3 500.0 478.9 pg of Hg 96% 77-123 1200625-CCV4 500.0 395.3 pg of Hg 79% 77-123 1200625-CCV5 500.0 450.8 pg of Hg 90% 77-123 1200625-CCV6 500.0 392.1 pg of Hg 78% 77-123

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Sample Containers

Lab ID: 

### 1232020-01 Report Matrix: 

### Influent Sample Type: 

### Sample Received: 

### 08/08/2012 Sample: 

### BioReactor 1 Inf Collected: 

### 08/06/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71666330 10 Cooler

none n/a 500 mL Bottle FLPE Hg-T

Lab ID: 

### 1232020-02 Report Matrix: 

### Influent Sample Type: 

### Sample Received: 

### 08/08/2012 Sample: 

### BioReactor 1 Inf Collected: 

### 08/06/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71659890 20 Cooler

none n/a 250 mL Bottle FLPE Hg-T Comments: 

### Split from THg Container

Lab ID: 

### 1232020-03 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 08/08/2012 Sample: 

### BioReactor 1 Inf Hg Blk Collected: 

### 08/06/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71666330 10 Cooler

none n/a 500 mL Bottle FLPE Hg-T

Lab ID: 

### 1232020-04 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 08/08/2012 Sample: 

### BioReactor 1 Inf Hg Blk Collected: 

### 08/06/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71659890 20 Cooler

none n/a 250 mL Bottle FLPE Hg-T Comments: 

### Split from THg Container

Lab ID: 

### 1232020-05 Report Matrix: 

### Influent Sample Type: 

### Sample Received: 

### 08/08/2012 Sample: 

### BioReactor 2 Inf Collected: 

### 08/06/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71666330 10 Cooler

none n/a 500 mL Bottle FLPE Hg-T

Lab ID: 

### 1232020-06 Report Matrix: 

### Influent Sample Type: 

### Sample Received: 

### 08/08/2012 Sample: 

### BioReactor 2 Inf Collected: 

### 08/06/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71659890 20 Cooler

none n/a 250 mL Bottle FLPE Hg-T Comments: 

### Split from THg Container

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Sample Containers

Lab ID: 

### 1232020-07 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 08/08/2012 Sample: 

### BioReactor 2 Inf Hg Blk Collected: 

### 08/06/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71666330 10 Cooler

none n/a 500 mL Bottle FLPE Hg-T

Lab ID: 

### 1232020-08 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 08/08/2012 Sample: 

### BioReactor 2 Inf Hg Blk Collected: 

### 08/06/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71659890 20 Cooler

none n/a 250 mL Bottle FLPE Hg-T Comments: 

### Split from THg Container

Lab ID: 

### 1232020-09 Report Matrix: 

### Effluent Sample Type: 

### Sample Received: 

### 08/08/2012 Sample: 

### BioReactor 2 Eff Collected: 

### 08/06/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71666330 10 Cooler

none n/a 500 mL Bottle FLPE Hg-T

Lab ID: 

### 1232020-10 Report Matrix: 

### Effluent Sample Type: 

### Sample Received: 

### 08/08/2012 Sample: 

### BioReactor 2 Eff Collected: 

### 08/06/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71659890 20 Cooler

none n/a 250 mL Bottle FLPE Hg-T Comments: 

### Split from THg Container

Lab ID: 

### 1232020-11 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 08/08/2012 Sample: 

### BioReactor 2 Eff Hg Blk Collected: 

### 08/06/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71666330 10 Cooler

none n/a 500 mL Bottle FLPE Hg-T

Lab ID: 

### 1232020-12 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 08/08/2012 Sample: 

### BioReactor 2 Eff Hg Blk Collected: 

### 08/06/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71659890 20 Cooler

none n/a 250 mL Bottle FLPE Hg-T Comments: 

### Split from THg Container

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Shipping Containers

Cooler Tracking No: 

### 5353 0519 2804 via FedEx Temperature: 

### -0.8 °C Coolant Type: 

### Ice Description: 

### Cooler Damaged in transit? 

### No Returned to client? 

### No Custody seals present? 

### No Custody seals intact? 

### No COC present? 

### Yes Received: 

### August 8, 2012 9:15

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

### This data package contains the analytical results for the project identified above and includes a Case Narrative, Sample Results and Chain of Custody. Unless otherwise noted, all samples were received in acceptable condition and processed according to the referenced methods. Data qualifiers are flagged individually on each sample. A key reference for the data qualifiers appears at the end of this case narrative. NC Certification No. 402 SC Certification No. 99012 NC Drinking Water Cert No. 37735 

# Case Narrative 08/13/2012

Duke Energy Corporation (04) Jay Perkins 13339 Hagers Ferry Road Huntersville, NC 28078 Project: Allen Shay/Miller Creek Project No.: J12080128 Lab Submittal Date: 08/07/2012 Prism Work Order: 2080159 VA Certification No. 1287



### Please call if you have any questions relating to this analytical report. Respectfully, PRISM LABORATORIES, INC.



### VP Laboratory Services Reviewed By

Data Qualifiers Key Reference: 

### HT Sample received and analyzed outside of the hold time.

Method Detection Limit MDL Below Reporting Limit BRL
Relative Percent Difference RPD


## * Results reported to the reporting limit. All other results are reported to the MDL with values between MDL and reporting limit indicated with a J.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 1 of 9

# Sample Receipt Summary 08/13/2012 

### Prism Work Order: 2080159 Client Sample ID Matrix Lab Sample ID Date Sampled Date Received 

### 2080159-01 08/06/12 2012017083/FGD Purge Eff 08/07/12 Water 2080159-02 08/06/12 2012017085/BioReactor 1 Inf 08/07/12 Water 2080159-03 08/06/12 2012017087/BioReactor 2 Inf 08/07/12 Water 2080159-04 08/06/12 2012017089/BioReactor 2 Eff 08/07/12 Water Samples received in good condition at 4.5 degrees C unless otherwise noted.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 2 of 9

# Laboratory Report 08/13/2012

Result Parameter Batch ID Report Limit Analysis Date/Time Method Dilution Factor Units MDL Analyst 

### 13339 Hagers Ferry Road Project No.: Project: Allen Shay/Miller Creek Duke Energy Corporation (04)
Huntersville, NC 28078 Attn: Jay Perkins Sample Matrix: Water Client Sample ID: Prism Sample ID: 2080159-01 Prism Work Order: 2080159 Time Collected: 08/06/12 08:03 Time Submitted: 08/07/12 16:25 2012017083/FGD Purge Eff


### J12080128

General Chemistry Parameters 7.2 *SM4500-H B P2H0162 1 8/8/12 11:30 JAB HT pH Units pH 74 *SM2320 B P2H0159 1 5.0 8/8/12 11:00 JAB mg/L 0.66 Total Alkalinity BRL *SM2320 B P2H0160 1 5.0 8/8/12 11:00 JAB mg/L 0.66 Carbonate Alkalinity 74 *SM2320 B P2H0161 1 5.0 8/8/12 11:00 JAB mg/L 0.66 Bicarbonate Alkalinity

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc. 449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 3 of 9

# Laboratory Report 08/13/2012

Result Parameter Batch ID Report Limit Analysis Date/Time Method Dilution Factor Units MDL Analyst 

### 13339 Hagers Ferry Road Project No.: Project: Allen Shay/Miller Creek Duke Energy Corporation (04)
Huntersville, NC 28078 Attn: Jay Perkins Sample Matrix: Water Client Sample ID: Prism Sample ID: 2080159-02 Prism Work Order: 2080159 Time Collected: 08/06/12 08:11 Time Submitted: 08/07/12 16:25 2012017085/BioReactor 1 Inf


### J12080128

General Chemistry Parameters 7.3 *SM4500-H B P2H0162 1 8/8/12 11:30 JAB HT pH Units pH 79 *SM2320 B P2H0159 1 5.0 8/8/12 11:00 JAB mg/L 0.66 Total Alkalinity BRL *SM2320 B P2H0160 1 5.0 8/8/12 11:00 JAB mg/L 0.66 Carbonate Alkalinity 79 *SM2320 B P2H0161 1 5.0 8/8/12 11:00 JAB mg/L 0.66 Bicarbonate Alkalinity

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc. 449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 4 of 9

# Laboratory Report 08/13/2012

Result Parameter Batch ID Report Limit Analysis Date/Time Method Dilution Factor Units MDL Analyst 

### 13339 Hagers Ferry Road Project No.: Project: Allen Shay/Miller Creek Duke Energy Corporation (04)
Huntersville, NC 28078 Attn: Jay Perkins Sample Matrix: Water Client Sample ID: Prism Sample ID: 2080159-03 Prism Work Order: 2080159 Time Collected: 08/06/12 08:20 Time Submitted: 08/07/12 16:25 2012017087/BioReactor 2 Inf


### J12080128

General Chemistry Parameters 7.0 *SM4500-H B P2H0162 1 8/8/12 11:30 JAB HT pH Units pH 280 *SM2320 B P2H0159 1 5.0 8/8/12 11:00 JAB mg/L 0.66 Total Alkalinity BRL *SM2320 B P2H0160 1 5.0 8/8/12 11:00 JAB mg/L 0.66 Carbonate Alkalinity 280 *SM2320 B P2H0161 1 5.0 8/8/12 11:00 JAB mg/L 0.66 Bicarbonate Alkalinity

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc. 449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 5 of 9

# Laboratory Report 08/13/2012

Result Parameter Batch ID Report Limit Analysis Date/Time Method Dilution Factor Units MDL Analyst 

### 13339 Hagers Ferry Road Project No.: Project: Allen Shay/Miller Creek Duke Energy Corporation (04)
Huntersville, NC 28078 Attn: Jay Perkins Sample Matrix: Water Client Sample ID: Prism Sample ID: 2080159-04 Prism Work Order: 2080159 Time Collected: 08/06/12 08:15 Time Submitted: 08/07/12 16:25 2012017089/BioReactor 2 Eff


### J12080128

General Chemistry Parameters 7.4 *SM4500-H B P2H0162 1 8/8/12 11:30 JAB HT pH Units pH 290 *SM2320 B P2H0159 1 5.0 8/8/12 11:00 JAB mg/L 0.66 Total Alkalinity BRL *SM2320 B P2H0160 1 5.0 8/8/12 11:00 JAB mg/L 0.66 Carbonate Alkalinity 290 *SM2320 B P2H0161 1 5.0 8/8/12 11:00 JAB mg/L 0.66 Bicarbonate Alkalinity

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc. 449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 6 of 98/13/12 

### Level II QC Report

13339 Hagers Ferry Road Project No: J12080128 Project: Allen Shay/Miller Creek Duke Energy Corporation (04)
Huntersville, NC 28078 Attn: Jay Perkins Prism Work Order: 2080159 Time Submitted: 8/7/2012 4:25:00PM

Result Limit

Reporting Units Level Spike Result

Source %REC %REC Limits RPD RPD Limit Notes Analyte General Chemistry Parameters - Quality Control

Batch P2H0159 - NO PREP Blank (P2H0159-BLK1) Prepared & Analyzed: 08/08/12 Total Alkalinity mg/L BRL 5.0 LCS (P2H0159-BS1) Prepared & Analyzed: 08/08/12 Total Alkalinity mg/L 245 5.0 250.0 90-110 98 LCS Dup (P2H0159-BSD1) Prepared & Analyzed: 08/08/12 Total Alkalinity mg/L 245 5.0 250.0 200 90-110 98 0 Duplicate (P2H0159-DUP1) Prepared & Analyzed: 08/08/12 Source: 2080159-04 Total Alkalinity mg/L 307 5.0 289 20 6 Batch P2H0160 - NO PREP Blank (P2H0160-BLK1) Prepared & Analyzed: 08/08/12 Carbonate Alkalinity mg/L BRL 5.0 LCS (P2H0160-BS1) Prepared & Analyzed: 08/08/12 Carbonate Alkalinity mg/L 245 5.0 90-110 LCS Dup (P2H0160-BSD1) Prepared & Analyzed: 08/08/12 Carbonate Alkalinity mg/L 245 5.0 200 90-110 0 Duplicate (P2H0160-DUP1) Prepared & Analyzed: 08/08/12 Source: 2080159-04 Carbonate Alkalinity mg/L BRL 5.0 BRL 20 Batch P2H0161 - NO PREP Blank (P2H0161-BLK1) Prepared & Analyzed: 08/08/12 Bicarbonate Alkalinity mg/L BRL 5.0

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 7 of 98/13/12 

### Level II QC Report

13339 Hagers Ferry Road Project No: J12080128 Project: Allen Shay/Miller Creek Duke Energy Corporation (04)
Huntersville, NC 28078 Attn: Jay Perkins Prism Work Order: 2080159 Time Submitted: 8/7/2012 4:25:00PM

Result Limit

Reporting Units Level Spike Result

Source %REC %REC Limits RPD RPD Limit Notes Analyte General Chemistry Parameters - Quality Control

Batch P2H0161 - NO PREP LCS (P2H0161-BS1) Prepared & Analyzed: 08/08/12 Bicarbonate Alkalinity mg/L 245 5.0 250.0 90-110 98 LCS Dup (P2H0161-BSD1) Prepared & Analyzed: 08/08/12 Bicarbonate Alkalinity mg/L 245 5.0 250.0 200 90-110 98 0 Duplicate (P2H0161-DUP1) Prepared & Analyzed: 08/08/12 Source: 2080159-04 Bicarbonate Alkalinity mg/L 307 5.0 289 20 6 Batch P2H0162 - NO PREP LCS (P2H0162-BS1) Prepared & Analyzed: 08/08/12 pH pH Units 6.91 6.860 99-101 101 Duplicate (P2H0162-DUP1) Prepared & Analyzed: 08/08/12 Source: 2080159-04 pH pH Units 7.37 7.36 10 0.1

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

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