Printing Date 2013-09-02 12:00:55 UTC Restriction of specific regulatory purposes Confidentiality Owner (TS 204240) 1-Propanesulfonic acid, 2-hydroxy-3-(2- propen-1-yloxy)-, sodium salt (1:1) / WIL Research Europe B.V. / 'sHertogenbosch / Netherlands Legal entity owner WIL Research Europe B.V. / 's-Hertogenbosch / Nethe rlands 

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# Endpoint study record: Short - term toxicity to aquatic

invertebrates. WIL Research Europe 500676

Administrative Data

Data source Reference



# Materials and methods Test guideline
GLP compliance 

# Test materials Identity of test material same as for substance def ined in section 1 (if not read-across) Test material identity
Test material form Details on test material UUID IUC5 - a67d4e33 - c1c1 - 4123 - ae8e - 4b38607e86fb Dossier UUID 0 Author BOL / WIL Research Europe B.V. / 's - Hertogenbosch / Netherlands Date 2013 - 09 - 02 11:59:24 UTC Remarks
Purpose flag key study; robust study summary Study result type experimental result Study period 09 April 2013 - 15 May 2013 Reliability 1 (reliable without restriction) Rationale for reliability incl. deficiencies This study has been performed according to EPA and OECD guidelines and according to GLP principles.

Reference type Author Year Title Bibliographic source Testing

laboratory Report

no. Owner

company Company

study no. Report

date study report L.M.

Bouwman, MSc. 2013 ACUTE TOXICITY

STUDY IN DAPHNIA MAGNA WITH 1- PROPANESULFONIC ACID, 2-HYDROXY-3- (2-PROPEN-1- YLOXY)-, SODIUM SALT (1:1) (STATIC) - WIL Research Europe B.V. Hambakenwetering7 5231 DD 'sHertogenbosch The Netherlands 500676 Society of Chemical Manufacturers and Affiliates 1850 M Street, NW Suite 700 WASHINGTON, DC 20036 USA - 2013- 07-18

Qualifier Guideline Deviations according to EPA OPPTS 850.1010 (Aquatic Invertebra te Acute Toxicity Test, Freshwater Daphnids) no according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) no yes

yes Identifier Identity CAS number 52556-42-0; 123-34-2 and 7732-18-5 other: clear colourless liquid Molecular formula: C6H11NaO5S Molecular weight: 218.22 Description: Clear colourless liquid Batch: 12E1101 Purity/Composition: Sodium-1-allyloxy-2- hydroxypropane sulphonate 3-allyl-1,2-propanediol Water CAS Number: 2556-42-0 123-34-2 7732-18-5 Percent: 38.64 2.26 

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## Analytical monitoring Details on sampling

Details on analytical methods

Vehicle Details on test solutions


# Test organisms Test organisms (species) Details on test organisms 59.1 Test substance storage: At room temperature in the dark Stability under storage conditions: Stable Expiry date: 13 August 2013 Hygroscopic: No Volatile: No Specific Gravity / Density: 1.17 Stability in water: 1 year
yes Samples for possible analysis were taken from both the control and the test concentration according to the schedule below. Frequency: at t=0 h and t=48 h Volume: 1.5 ml from the approximate centre of the t est vessels Storage: Samples were stored in a freezer until ana lysis. At the end of the exposure period, the replicates w ere pooled at each concentration before sampling. Additionally, reserve samples of 1.5 ml were taken for possible analysis. If not used, these samples w ere stored in a freezer for a maximum of three months after delivery of the draft report, pe nding on the decision of the sponsor for additional analysis. The samples were stored in the freezer ( ≤ -15°C). Storage stability under these conditions w as demonstrated in WIL Research project 500674. On the day of analysis, samples were defrosted at r oom temperature and analysed. If necessary, the sam ples were diluted with ISO-medium to obtain concentrations within the calibration ran ge. Quantitative analysis was based on the analytical m ethod validated for the test substance in WIL Resea rch project 500674 Analytical method: Instrument: Alliance Separation Module 2695 (Waters , Milford, MA, USA) Detector: Refractive Index Detector 2414 (Waters) Column: Stationary phase: Synergi Hydro-RP 80A Dimensions: 250 mm x 4.6 mm i.d., dp =4 μm Brand: Phenomenex, Torrance, CA, USA Column temperature: 40°C +/- 1°C Injection volume: 50 μl Mobile phase: water containing 0.05M Na2HPO4, 0.05M NaH2PO4 and 0.05M NaNO3 Flow: 1 ml/min RI detection Temperature: 30°C Sensitivity: 4 Time constant: 1 seconds

no The batch of 1-Propanesulfonic acid, 2-hydroxy-3-(2 -propen-1-yloxy)-, sodium salt (1:1) tested was a c lear colourless liquid and completely soluble in test medium at the concentration tested. Preparation of test solutions started with a nomina l concentration of 1000 mg/l, containing 386 mg of the active ingredient (a.i.) Sodium-1- allyloxy-2-hydroxypropane sulphonate. Approximately fifteen minutes of magnetic stirring was applied t o accelerate the dissolving of the test substance in the test medium. The lower test concen trations for the range-finding test were prepared b y subsequent dilutions of the 1000 mg/l concentration in test medium. The final test soluti ons were all clear and colourless.
Daphnia magna Species: Daphnia magna (Crustacea, Cladocera) (Stra us, 1820), at least third generation, obtained by a cyclical parthenogenesis under specified breeding conditions. Source: In-house laboratory culture with a known hi story. Reason for selection: This system has been selected as an internationally accepted invertebrate specie s. Validity of batch: Daphnids originated from a healt hy stock, 2nd to 5th brood, showing no signs of str ess such as mortality >20%, presence of males, ephippia or discoloured animals and there wa s no delay in the production of the first brood. Characteristics: For the test selection of young da phnids with an age of < 24 hours, from parental dap hnids of more than two weeks old. Start of each batch: With newborn daphnids, i.e. le ss than 3 days old, by placing about 250 of them in to 5 litres of medium in an all-glass culture vessel. Maximum age of the cultures: 4 weeks Renewal of the cultures: After 7 days of cultivatio n half of the medium twice a week. 

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# Study design Test type Water media type Limit test Total exposure duration Post exposure observation period 

# Test conditions Hardness Test temperature pH Dissolved oxygen Salinity Nominal and measured concentrations
Details on test conditions

Reference substance (positive control) 

# Results and discussions Effect concentrations

Details on results

Results with reference substance Feeding: Daily, a suspension of fresh water algae.

static freshwater yes 48 h
none
180 mg/l expressed as CaCO3 19.4 - 20.3°C 7.6 - 7.9 8.8 - 9.2 mg/l not measured Nominal concentration: 1000 mg/l, containing 386 mg a.i./l The actual test concentration was in agreement with nominal (100%). This concentration remained stable during the test period (102% of initial at the end of the test). Given these results, the e ffect parameters were based on the nominal exposure concentration. Test duration: 48 hours Test type: static Test vessel: 100 ml, all glass Medium: Adjusted ISO-medium Number of daphnids: 20 in the control and the test concentration. Loading: 5 per vessel containing 80 ml of test solu tion Light: 16h light 8h dark Feeding: No feeding Aeration: No aeration of the test solutions Introduction of the daphnids: Within 20 minutes aft er preparation of the test solutions. yes (potassium dichromate)
Duration Endpoint Effect conc. Nominal/Measured Conc. based on Basis for effect Remarks (e.g. 95% CL) 24 h EC50 > 1000 mg/L nominal test mat. (analytically confirmed nominal concentration ) mobility EC50: >386 mg active ingredient /L 48 h EC50 > 1000 mg/L nominal test mat. (analytically confirmed nominal concentration) mobility EC50: >386 mg active ingredient /L Behavioural abnormaliies - Other biological observations: none - Mortality of control: no - Other adverse effects control: no -Results test substance valid? Yes -EC50: The 48h-EC50 was 0.53 mg/l with a 95% confid ence interval between 0.49 and 0.62 mg/l. 

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## Reported statistics and error estimates



# Applicant's summary and conclusion Validity criteria fulfilled Conclusions No EC50 could be calculated because the test substa nce proved to be non-toxic (EC50 > maximum concentr ation tested).

yes 1-Propanesulfonic acid, 2-hydroxy-3-(2-propen-1-ylo xy)-, sodium salt (1:1) did not induce acute immobi lisation of Daphnia magna at an analytically confirmed nominal concentration of 100 0 mg/l. This concentration contained 386 mg of the active ingredient per litre. Hence, the 48h-EC50 was >1000 mg test substance/l, corresponding to >386 mg a.i./l. 

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