Printing Date 2013-09-02 12:00:55 UTC 

Restriction of specific regulatory purposes 

 

Confidentiality 

 

Owner (TS 204240) 1-Propanesulfonic acid, 2-hydroxy-3-(2-propen-1-yloxy)-, sodium salt (1:1) / WIL Research Europe B.V. / 'sHertogenbosch / Netherlands 

Legal entity 

owner 

WIL Research Europe B.V. / 's-Hertogenbosch / Netherlands 

Endpoint study record: Short-term toxicity to aquatic invertebrates. WIL Research Eur... Page 1 of 5

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Endpoint study record: Short-term toxicity to aquatic 

invertebrates. WIL Research Europe 500676 

Administrative Data 

Data source 

Reference 

Materials and methods 

Test guideline 

GLP compliance 

Test materials 

Identity of test material same as for substance defined in section 1 (if not read-across) 

Test material identity 

Test material form 

Details on test material 

UUID 

 

IUC5-a67d4e33-c1c1-4123-ae8e-4b38607e86fb 

Dossier UUID 

 

0 

Author 

 

BOL / WIL Research Europe B.V. / 's-Hertogenbosch / Netherlands 

Date 

 

2013-09-02 11:59:24 UTC 

Remarks 

 

 

Purpose flag key study; robust study summary 

Study result type experimental result Study period 09 April 2013 - 15 May 2013 

Reliability 1 (reliable without restriction) 

Rationale for reliability incl. 

deficiencies 

This study has been performed according to EPA and OECD guidelines and according to GLP 

principles. 

Reference 

type 

Author Year Title Bibliographic 

source 

Testing 

laboratory 

Report 

no. 

Owner 

company 

Company 

study no. 

Report 

date 

study 

report 

L.M. 

Bouwman, 

MSc. 

2013 ACUTE TOXICITY 

STUDY IN DAPHNIA 

MAGNA WITH 1-

PROPANESULFONIC 

ACID, 2-HYDROXY-3-

(2-PROPEN-1-

YLOXY)-, SODIUM 

SALT (1:1) (STATIC) 

- WIL Research 

Europe B.V. 

Hambakenwetering 

7 5231 DD 'sHertogenbosch 

The Netherlands 

500676 Society of 

Chemical 

Manufacturers 

and Affiliates 

1850 M Street, 

NW Suite 700 

WASHINGTON, 

DC 20036 USA 

- 2013-

07-18 

Qualifier Guideline Deviations 

according to EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids) no 

according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) no 

yes 

yes 

Identifier Identity 

CAS number 52556-42-0; 123-34-2 and 7732-18-5 

other: clear colourless liquid 

Molecular formula: C6H11NaO5S 

Molecular weight: 218.22 

Description: Clear colourless liquid 

Batch: 12E1101 

Purity/Composition: 

Sodium-1-allyloxy-2- hydroxypropane sulphonate 

3-allyl-1,2-propanediol 

Water 

CAS Number: 

2556-42-0 

123-34-2 

7732-18-5 

Percent: 

38.64 

2.26 

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Analytical monitoring 

Details on sampling 

Details on analytical methods 

Vehicle 

Details on test solutions 

Test organisms 

Test organisms (species) 

Details on test organisms 

59.1 

Test substance storage: At room temperature in the dark

Stability under storage conditions: Stable 

Expiry date: 13 August 2013 

Hygroscopic: No 

Volatile: No 

Specific Gravity / Density: 1.17 

Stability in water: 1 year 

yes 

Samples for possible analysis were taken from both the control and the test concentration according to the schedule below. 

Frequency: at t=0 h and t=48 h 

Volume: 1.5 ml from the approximate centre of the test vessels 

Storage: Samples were stored in a freezer until analysis. 

At the end of the exposure period, the replicates were pooled at each concentration before sampling. 

Additionally, reserve samples of 1.5 ml were taken for possible analysis. If not used, these samples were stored in a freezer for a maximum of 

three months after delivery of the draft report, pending on the decision of the sponsor for additional analysis. 

The samples were stored in the freezer (≤ -15°C). Storage stability under these conditions was demonstrated in WIL Research project 500674.

On the day of analysis, samples were defrosted at room temperature and analysed. If necessary, the samples were diluted with ISO-medium 

to obtain concentrations within the calibration range. 

Quantitative analysis was based on the analytical method validated for the test substance in WIL Research project 500674 

Analytical method: 

Instrument: Alliance Separation Module 2695 (Waters, Milford, MA, USA) 

Detector: Refractive Index Detector 2414 (Waters) 

Column: 

Stationary phase: Synergi Hydro-RP 80A 

Dimensions: 250 mm x 4.6 mm i.d., dp =4 μm 

Brand: Phenomenex, Torrance, CA, USA 

Column temperature: 40°C +/- 1°C 

Injection volume: 50 μl 

Mobile phase: water containing 0.05M Na2HPO4, 0.05M NaH2PO4 and 0.05M NaNO3 

Flow: 1 ml/min 

RI detection 

Temperature: 30°C 

Sensitivity: 4 

Time constant: 1 seconds 

no 

The batch of 1-Propanesulfonic acid, 2-hydroxy-3-(2-propen-1-yloxy)-, sodium salt (1:1) tested was a clear colourless liquid and completely 

soluble in test medium at the concentration tested.

Preparation of test solutions started with a nominal concentration of 1000 mg/l, containing 386 mg of the active ingredient (a.i.) Sodium-1-

allyloxy-2-hydroxypropane sulphonate. Approximately fifteen minutes of magnetic stirring was applied to accelerate the dissolving of the test 

substance in the test medium. The lower test concentrations for the range-finding test were prepared by subsequent dilutions of the 1000 mg/l 

concentration in test medium. The final test solutions were all clear and colourless. 

Daphnia magna 

Species: Daphnia magna (Crustacea, Cladocera) (Straus, 1820), at least third generation, obtained by acyclical parthenogenesis under 

specified breeding conditions. 

Source: In-house laboratory culture with a known history. 

Reason for selection: This system has been selected as an internationally accepted invertebrate species. 

Validity of batch: Daphnids originated from a healthy stock, 2nd to 5th brood, showing no signs of stress such as mortality >20%, presence of 

males, ephippia or discoloured animals and there was no delay in the production of the first brood. 

Characteristics: For the test selection of young daphnids with an age of < 24 hours, from parental daphnids of more than two weeks old. 

Start of each batch: With newborn daphnids, i.e. less than 3 days old, by placing about 250 of them into 5 litres of medium in an all-glass 

culture vessel. 

Maximum age of the cultures: 4 weeks 

Renewal of the cultures: After 7 days of cultivation half of the medium twice a week. 

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Study design 

Test type 

Water media type 

Limit test 

Total exposure duration 

Post exposure observation period 

Test conditions 

Hardness 

Test temperature 

pH 

Dissolved oxygen 

Salinity 

Nominal and measured concentrations 

Details on test conditions 

Reference substance (positive control) 

Results and discussions 

Effect concentrations 

Details on results 

Results with reference substance 

Feeding: Daily, a suspension of fresh water algae. 

static 

freshwater 

yes 

48 h 

none 

180 mg/l expressed as CaCO3 

19.4 - 20.3°C 

7.6 - 7.9 

8.8 - 9.2 mg/l 

not measured 

Nominal concentration: 1000 mg/l, containing 386 mg a.i./l 

The actual test concentration was in agreement with nominal (100%). This concentration remained stable during the test period (102% of initial 

at the end of the test). Given these results, the effect parameters were based on the nominal exposure concentration. 

Test duration: 48 hours 

Test type: static 

Test vessel: 100 ml, all glass 

Medium: Adjusted ISO-medium 

Number of daphnids: 20 in the control and the test concentration. 

Loading: 5 per vessel containing 80 ml of test solution 

Light: 16h light 8h dark 

Feeding: No feeding 

Aeration: No aeration of the test solutions 

Introduction of the daphnids: Within 20 minutes after preparation of the test solutions. 

yes (potassium dichromate) 

Duration Endpoint Effect 

conc. 

Nominal/Measured Conc. based on Basis for 

effect 

Remarks (e.g. 95% CL) 

24 h EC50 > 1000 

mg/L 

nominal test mat. (analytically confirmed nominal 

concentration ) 

mobility EC50: >386 mg active 

ingredient /L 

48 h EC50 > 1000 

mg/L 

nominal test mat. (analytically confirmed nominal 

concentration) 

mobility EC50: >386 mg active 

ingredient /L 

Behavioural abnormaliies 

- Other biological observations: none

- Mortality of control: no 

- Other adverse effects control: no 

-Results test substance valid? Yes 

-EC50: The 48h-EC50 was 0.53 mg/l with a 95% confidence interval between 0.49 and 0.62 mg/l. 

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Reported statistics and error estimates 

Applicant's summary and conclusion 

Validity criteria fulfilled 

Conclusions 

No EC50 could be calculated because the test substance proved to be non-toxic (EC50 > maximum concentration tested). 

yes 

1-Propanesulfonic acid, 2-hydroxy-3-(2-propen-1-yloxy)-, sodium salt (1:1) did not induce acute immobilisation of Daphnia magna at an 

analytically confirmed nominal concentration of 1000 mg/l. This concentration contained 386 mg of the active ingredient per litre. 

Hence, the 48h-EC50 was >1000 mg test substance/l, corresponding to >386 mg a.i./l. 

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