# Patent

## Title
Isoindoline inhibitors of ROR-gamma

## Abstract

Provided are novel compounds of Formula (I):




pharmaceutically acceptable salts thereof, and pharmaceutical compositions thereof, which are useful in the treatment of diseases and disorders mediated by RORγ. Also provided are pharmaceutical compositions comprising the novel compounds of Formula (I) and methods for their use in treating one or more inflammatory, metabolic, autoimmune and other diseases or disorders.

## Background
RELATED APPLICATIONS
This application claims the benefit of U.S. Provisional Application No. 62/075,612, filed Nov. 5, 2014, the entire contents of which is incorporated herein by reference.


TECHNICAL FIELD OF THE INVENTION
The present invention is directed to novel retinoic acid receptor-related orphan receptor gamma (“RORγ” or “ROR-gamma”) inhibitors, processes for their preparation, pharmaceutical compositions containing these inhibitors, and their use in the treatment of inflammatory, metabolic, autoimmune and other diseases mediated by RORγ.
BACKGROUND OF THE INVENTION
Retinoic acid receptor-related orphan receptors (RORs) are a subfamily of transcription factors in the steroid hormone nuclear receptor superfamily (Jetten & Joo (2006) Adv. Dev. Biol. 2006, 16, 313-355). The ROR family consists of ROR alpha (RORα), ROR beta (RORβ) and ROR gamma (RORγ), each encoded by a separate gene (in human: RORA, RORB and RORC, respectively; in mouse: rora, rorb and rorc, respectively). RORs contain four principal domains shared by the majority of nuclear receptors: an N-terminal domain, a highly conserved DNA-binding domain (DBD) consisting of two zinc finger motifs, a hinge domain, and a ligand binding domain (LBD). Each ROR gene generates several isoforms, differing only in their N-terminal domains. RORγ has two isoforms: RORγ1 and RORγ2 (also known as RORγt). RORγ refers to RORγ1 and/or RORγt. RORγ1 is expressed in a variety of tissues including thymus, muscle, kidney and liver, but RORγt is exclusively expressed in the cells of the immune system, has a critical role in thymopoiesis and the development of several secondary lymphoid tissues, and is a key regulator of Th17 cell differentiation (Jetten, 2009, Nucl. Recept. Signal., 7:e003, doi:10.1621/nrs.07003, Epub 2009 Apr. 3).
Th17 cells are a subset of T helper cells which preferentially produce the pro-inflammatory cytokines IL-17A, IL-17F, IL-21 and IL-22. Th17 cells and their effector molecules, such as IL-17, IL-21, IL-22, GM-CSF and CCL20, are associated with the pathogenesis of several autoimmune and inflammatory diseases, such as rheumatoid arthritis, systemic lupus erythematosus, multiple sclerosis, psoriasis, inflammatory bowel disease, allergy and asthma (Maddur et al., 2012, Am. J. Pathol., 181:8-18). Recent findings support a role for IL17 and Th17 cells in the pathogenesis of acne (Thiboutot et al., 2014, J. Invest. Dermatol., 134(2):307-10, doi: 10.1038/jid.2013.400; Agak et al., 2014, J. Invest. Dermatol., 134(2):366-73, doi: 10.1038/jid.2013.334, Epub 2013 Aug. 7). Th17 cells are also potent inducers of inflammation associated with endometriosis, a chronic inflammatory disease (Hirata et al., 2010, Endocrinol., 151:5468-5476; Hirata et al., 2011, Fertil Steril., July; 96(1):113-7, doi: 10.1016/j.fertnstert.2011.04.060, Epub 2011 May 20). Additionally, Th17 cells have a key role in the mouse autoimmune models of experimental autoimmune encephalomyelitis (EAE), collagen-induced arthritis (CIA) and adjuvant-induced arthritis (AIA) (Bedoya et al., 2013, Clin. Dev. Immunol., 2013: 986789. Epub 2013 Dec. 26. Th17 cells are activated during inflammatory and autoimmune disease processes and are responsible for recruiting other inflammatory cell types, particularly neutrophils, to mediate pathology in target tissues (Miossec & Kolls, 2012, Nature Rev., 11:763-776; Korn et al., 2009, Annu. Rev. Immunol., 27:485-517). Aberrant Th17 cell function has been implicated in a variety of autoimmune diseases, including multiple sclerosis and rheumatoid arthritis. Autoimmune disease is believed to arise from the disruption of the equilibrium between effector and regulatory T cells (Solt et al., 2012, ACS Chem. Biol., 7:1515-1519, Epub 2012 Jul. 9). The importance of RORγt to Th17 cell differentiation and the pathogenic role of Th17 cells is evidenced by the fact that RORγt-deficient mice have very few Th17 cells and have a reduction in severity of EAE (Ivanov et al., 2006, Cell, 126:1121-1133).
Circadian rhythms are daily cycles of behavioral and physiological changes that are regulated by endogenous circadian clocks. A number of studies have established links between nuclear receptor (including RORγ) function and expression, the circadian regulatory circuitry, and the regulation of various physiological processes (Jetten (2009) op. cit.).
Obstructive sleep apnea syndrome (OSAS) is a chronic inflammatory disease regulated by T lymphocytes. OSAS patients have a significant increase in peripheral Th17 cell frequency, IL-17 and RORγt levels (Ye et al., 2012, Mediators Inflamm., 815308, doi: 10.1155/2012/815308, Epub 2012 Dec. 31).
A number of studies have provided evidence of a role of RORs in cancer. Mice deficient in the expression of RORγ exhibit a high incidence of thymic lymphomas that metastasize frequently to liver and spleen. High expression of Th17-associated genes (including RORγ) and high levels of Th17 cells in the tumor microenvironment has been shown to correlate with a poor prognosis in various cancers, including lung, gastric, breast and colon cancer (Tosolini et al., 2011, Cancer Res., 71:1263-1271, doi: 10.1158/0008-5472.CAN-10-2907, Epub 2011 Feb. 8; Su et al., 2014, Immunol. Res., 58:118-124, doi: 10.1007/s12026-013-8483-y, Epub 2014 Jan. 9; Carmi et al., 2011, J. Immunol., 186:3462-3471, doi: 10.4049/jimmunol.1002901, Epub 2011 Feb. 7; Chen et al., 2013, Histopathology, 63:225-233, doi: 10.1111/his.12156, Epub 2013 Jun. 6).
RORγ has also been identified to have a regulatory role in lipid/glucose homeostasis, and has been implicated in metabolic syndrome, obesity (Meissburger et al., 2011, EMBO Mol. Med., 3:637-651), hepatosteatosis, insulin resistance and diabetes.
Further support for the role of RORγ in the pathogenesis of inflammatory, metabolic, circadian effect, cancer, and autoimmune diseases and disorders can be found in the following references: Chang et al., 2012, J. Exp. Pharmacol., 4:141-148; Jetten et al., 2013, Frontiers Endocrinol., 4:1-8; Huh & Littman, 2012, Eur. J. Immunol., 42:2232-2237; Martinez et al., 2008, Ann. N.Y. Acad. Sci., 1143:188-211; Pantelyushin et al., 2012, J. Clin. Invest., 122:2252-2256; Jetten & Ueda, 2002, Cell Death Differen., 9:1167-1171; Solt et al., 2010, Curr. Opin. Lipidol., 21:204-211.
In light of the role that RORγ plays in disease pathogenesis, inhibition of RORγ activity and Th17 cell differentiation and activity, including IL17 production, will be of significant therapeutic benefit. It is therefore desirable to prepare compounds that inhibit RORγ activity and hence have utility in the treatment of inflammatory, autoimmune, metabolic, circadian effect, cancer, and other diseases mediated by RORγ, such as e.g., asthma, atopic dermatitis, acne, Crohn's disease, regional enteritis, ulcerative colitis, Sjögren's syndrome, uveitis, Behçet's disease, dermatomyositis, multiple sclerosis, ankylosing spondylitis, systemic lupus erythematosus, scleroderma, psoriasis, psoriatic arthritis, steroid resistant asthma and rheumatoid arthritis.
SUMMARY OF THE INVENTION
It has now been found that compounds described herein, and pharmaceutically acceptable compositions thereof, are effective inhibitors of RORγ (see e.g., Table 4). Such compounds include those of Formula (I):




or a pharmaceutically acceptable salt thereof, wherein each of R2, R3, R4, X, L1, n, m, Cy1, and Cy2 are as defined and described herein.

The provided compounds, and pharmaceutically acceptable compositions thereof, are inverse agonists or antagonists of RORγ and are useful for treating a variety of diseases, disorders or conditions. Such diseases, disorders, or conditions include those described herein.
The provided compounds can be used alone (i.e., as a monotherapy) or in combination with one or more other therapeutic agent effective for treating any of the indications described herein.


DETAILED DESCRIPTION OF CERTAIN EMBODIMENTS
1. General Description of Compounds of the Invention
In certain embodiments, the present invention provides a compound of Formula (I):




or a pharmaceutically acceptable salt thereof, wherein:

R2 is hydroxy, monocyclic cycloalkyl, monocyclic heterocyclyl, or (C1-C6)alkyl, wherein the (C1-C6)alkyl is optionally substituted with 1 to 2 groups independently selected from hydroxy, halo, (C1-C3)alkoxy, and cyano;
R3 is hydrogen, monocyclic cycloalkyl, monocyclic heterocyclyl, or (C1-C6)alkyl, wherein the (C1-C6)alkyl is optionally substituted with 1 to 2 groups independently selected from hydroxy, halo, and cyano;
R4 is hydrogen, (C1-C3)alkyl, or ═O;
X is —C(O)NH— or —NHC(O)—;
m is 0, 1, or 2;
n is 0, 1, 2, or 3;
L1 is absent or is SO2 or CR7R8;
Cy1 is aryl, heteroaryl, heterocyclyl, or cycloalkyl, each of which is optionally substituted with 1 to 3 groups independently selected from R5;
Cy2 is aryl, heteroaryl, monocyclic cycloalkyl, monocyclic heterocyclyl, or bicyclic heterocyclyl, each of which is optionally substituted with 1 to 3 groups independently selected from R6;
R5 and R6 are each independently selected from halo, cyano, nitro, amino, hydroxy, carboxy, (C1-C6)alkyl, heterocyclyl, hydroxy(C1-C6)alkyl, CO2H, (CH2)1-3COOH, (C1-C3)alkylcarbonyloxy, (C3-C6)cycloalkyl, hydroxy(C3-C6)cycloalkyl, (C4-C7)cycloalkylalkyl, (C2-C6)alkenyl, halo(C2-C6)alkenyl, hydroxy(C2-C6)alkenyl, (C2-C6)alkynyl, (C3-C6)cycloalkyl(C2-C4)alkynyl, halo(C1-C6)alkyl, halo(C3-C6)cycloalkyl, halo(C4-C7)cycloalkylalkyl, (C1-C6)alkoxy, (C3-C6)cycloalkoxy, (C4-C7)cycloalkylalkoxy, halo(C1-C6)alkoxy, halo(C3-C6)cycloalkoxy, halo(C4-C7)cycloalkylalkoxy, (C1-C6)alkylthio, (C3-C6)cycloalkythio, (C4-C7)cycloalkylalkylthio, halo(C1-C6)alkylthio, halo(C3-C6)cycloalkythio, halo(C4-C7)cycloalkylalkylthio, (C1-C6)alkylsulfinyl, (C3-C6)cycloalkylsulfinyl, (C4-C7)cycloalkylalkylsulfinyl, halo(C1-C6)alkylsulfinyl, halo(C3-C6)cycloalkylsulfinyl, halo(C4-C7)cycloalkylalkylsulfinyl, (C1-C6)alkylsulfonyl, (C3-C6)cycloalkylsulfonyl, (C4-C7)cycloalkylalkylsulfonyl, halo(C1-C6)alkylsulfonyl, hydroxy(C2-C6)alkylsulfonyl, halo(C3-C6)cycloalkylsulfonyl, halo(C4-C7)cycloalkylalkylsulfonyl, (C1-C6)alkylamino, di(C1-C6)alkylamino, (C1-C6)alkoxy(C1-C6)alkoxy, halo(C1-C6)alkoxy(C1-C6)alkoxy, (C1-C6)alkoxycarbonyl, H2NCO, H2NSO2, (C1-C6)alkylaminocarbonyl, di(C1-C6)alkylaminocarbonyl, (C1-C3)alkoxy(C1-C3)alkylaminocarbonyl, heterocyclylcarbonyl, (C1-C6)alkylamino sulfonyl, di(C1-C6)alkylaminosulfonyl, heterocyclylsulfonyl, (C1-C6)alkylcarbonylamino, (C1-C6)alkylcarbonylamino(C1-C6)alkyl, (C1-C6)alkylsulfonylamino, (C1-C6)alkylsulfonylamino(C1-C6)alkyl, (C1-C6)alkoxycarbonyl(C1-C6)alkoxy, (C1-C6)alkoxy(C1-C6)alkyl, halo(C1-C6)alkoxy(C1-C6)alkyl, hydroxy(C1-C6)alkoxy, aryl, heteroaryl, oxo, amino(C1-C6)alkyl, (C1-C6)alkylamino(C1-C6)alkyl, di(C1-C6)alkylamino(C1-C6)alkyl amino(C2-C6)alkoxy, (C1-C6)alkylamino(C2-C6)alkoxy, di(C1-C6)alkylamino(C2-C6)alkoxy, (C1-C6)alkylcarbonyl, hydroxy(C1-C6)alkylcarbonyl, (C1-C6)alkylhydroxycarbonyl, (C1-C6)alkylhydroxy(C1-C6)alkyl, (C3-C6)cycloalkylcarbonyl, (C3-C6)cycloalkylaminocarbonyl, {(C3-C6)cycloalkyl}{(C1-C6)alkyl}aminocarbonyl, di(C3-C6)cycloalkylaminocarbonyl, (C3-C6)cycloalkylaminosulfonyl, {(C3-C6)cycloalkyl}{(C1-C6)alkyl}amino sulfonyl, di(C3-C6)cycloalkylaminosulfonyl, cyano(C1-C6)alkyl, aminocarbonyl(C1-C6)alkyl, (C1-C6)alkylaminocarbonyl(C1-C6)alkyl, di(C1-C6)alkylaminocarbonyl(C1-C6)alkyl, (C3-C6)cyclo alkylaminocarbonyl(C1-C6)alkyl, {(C3-C6)cycloalkyl}{(C1-C6)alkyl}aminocarbonyl(C1-C6)alkyl, [(C1-C6)alkyl(C4-C6)heterocyclyl](C1-C6)alkyl, and di(C3-C6)cycloalkylaminocarbonyl(C1-C6)alkyl; and
R7 and R8 are each independently hydrogen, hydroxy, (C1-C3)alkyl, hydroxy(C1-C3)alkyl, (C1-C3)alkoxy(C1-C3)alkyl, aminocarbonyl-O(C1-C3)alkyl, hydroxycarbonyl(C1-C3)alkoxy(C1-C3)alkyl, (C1-C3)alkylcarbonylamino(C1-C3)alkyl, (C1-C3)alkoxycarbonylamino(C1-C3)alkyl, di(C1-C3)alkylamino(C1-C3)alkyl, di(C1-C3)alkylaminocarbonyl(C1-C3)alkyl, (C1-C3)alkylaminocarbonyl(C1-C3)alkyl, aminocarbonyl(C1-C3)alkyl, aminocarbonyl, mono(C1-C3)alkylamino, di(C1-C3)alkylamino, CO2H, (CH2)1-3COOH, moncyclic heterocyclyl, (C1-C3)alkoxycarbonyl, halophenyl, halophenyl(C1-C3)alkyl, or quinolin-2(1H)one-4yl-methyl; or
R7 and R8, together with the carbon atom to which they are attached, form a 3- to 6-membered cycloalkyl or heterocyclyl.
2. Compounds and Definitions
The terms “halo” and “halogen” as used herein refer to an atom selected from fluorine (fluoro, F), chlorine (chloro, —Cl), bromine (bromo, —Br), and iodine (iodo, —I).
The term “alkyl”, used alone or as a part of a larger moiety such as e.g., “haloalkyl”, means a saturated monovalent straight or branched hydrocarbon radical having, unless otherwise specified, 1-10 carbon atoms and includes, for example, methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, isobutyl, tert-butyl, n-pentyl, n-hexyl, n-heptyl, n-octyl, n-nonyl, n-decyl and the like. “Monovalent” means attached to the rest of the molecule at one point.
The term “haloalkyl” or “halocycloalkyl” include mono, poly, and perhaloalkyl groups where the halogens are independently selected from fluorine, chlorine, iodine, and bromine.
The term “cycloalkyl” used alone or as part of a larger moiety, refer to a saturated aliphatic monocyclic or bicyclic ring system, as described herein, having from, unless otherwise specified, 3 to 10 carbon ring atoms. Monocyclic cycloalkyl groups include, without limitation, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl. It will be understood that when specified, optional substituents on a cycloalkyl group may be present on any substitutable position and, include, e.g., the position at which the cycloalkyl group is attached.
The term “cycloaliphatic” used alone or as part of a larger moiety refer to unsaturated non-aromatic monocyclic or bicyclic ring system, as described herein, having from, unless otherwise specified, 3 to 10 carbon ring atoms. Monocyclic cycloaliphatic groups include, without limitation, cyclopentenyl, cyclohexenyl, and cycloheptenyl. It will be understood that when specified, optional substituents on a cycloaliphatic group may be present on any substitutable position and, include, e.g., the position at which the cycloaliphatic group is attached.
The term “carbocycle”, “carbocyclyl”, “carbocyclo”, or “carbocyclic” used alone or as part of a larger moiety refer to saturated, partially saturated, or aromatic ring systems comprising all carbon atoms having, unless otherwise specified, a total of 3 to 10 ring members. It will be understood that when specified, optional substituents on a carbocycle, carbocyclyl, carbocyclo, or carbocyclic may be present on any substitutable position and, include, e.g., the position at which the carbocycle is attached.
The term “aryl” used alone or as part of a larger moiety as in “aralkyl”, “aralkoxy”, or “aryloxyalkyl”, refers to an aromatic carbocyclic ring system having, unless otherwise specified, a total of 6 to 10 ring members. The term “aryl” may be used interchangeably with the term “aryl ring”, “aryl group”, “aryl moiety,” or “aryl radical”. In certain embodiments of the present disclosure, “aryl” refers to an aromatic ring system which includes, but is not limited to, phenyl (abbreviated as “Ph”), naphthyl and the like. It will be understood that when specified, optional substituents on an aryl group may be present on any substitutable position and, include, e.g., the position at which the aryl is attached.
The term “heteroaryl” used alone or as part of a larger moiety as in “heteroarylalkyl”, “heteroarylalkoxy”, or “heteroarylaminoalkyl”, refers to a 5-10-membered aromatic radical containing 1-4 heteroatoms selected from N, O, and S and includes, for example, thienyl, furanyl, pyrrolyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, oxazolyl, isoxazolyl, oxadiazolyl, thiazolyl, isothiazolyl, thiadiazolyl, pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, indolizinyl, purinyl, naphthyridinyl, and pteridinyl. The term “heteroaryl” may be used interchangeably with the terms “heteroaryl ring”, “heteroaryl group”, or “heteroaromatic”. The terms “heteroaryl” and “heteroar-”, as used herein, also include groups in which a heteroaromatic ring is fused to one or more aryl rings, where the radical or point of attachment is on the heteroaromatic ring. Nonlimiting examples include indolyl, indazolyl, benzimidazolyl, benzthiazolyl, quinolyl, quinazolinyl, imidazopyrimidinyl and quinoxalinyl. A heteroaryl group may be mono- or bicyclic. It will be understood that when specified, optional substituents on a heteroaryl group may be present on any substitutable position and, include, e.g., the position at which the heteroaryl is attached.
The term “heterocyclyl” means a 4-, 5-, 6- and 7-membered saturated or partially unsaturated heterocyclic ring containing 1 to 4 heteroatoms independently selected from N, O, and S. The terms “heterocycle”, “heterocyclyl”, “heterocyclyl ring”, “heterocyclic group”, “heterocyclic moiety”, and “heterocyclic radical”, are used interchangeably herein. A heterocyclyl ring can be attached to its pendant group at any heteroatom or carbon atom that results in a stable structure. Examples of such saturated or partially unsaturated heterocyclic radicals include, without limitation, tetrahydrofuranyl, tetrahydrothienyl, tetrahydropyranyl, pyrrolidinyl, pyrrolidonyl, piperidinyl, oxazolidinyl, piperazinyl, dioxanyl, dioxolanyl, morpholinyl, dihydrofuranyl, dihydropyranyl, dihydropyridinyl, tetrahydropyridinyl, dihydropyrimidinyl, and tetrahydropyrimidinyl. A heterocyclyl group may be mono- or bicyclic. Unless otherwise specified, bicyclic heterocyclyl groups include, e.g., unsaturated heterocyclic radicals fused to another unsaturated heterocyclic radical or aromatic, cycloalkyl, or heteroaryl ring, such as for example, tetrahydronaphthyridine, 4,5,6,7-tetrahydrobenzo[d]oxazolyl, indolinone, dihydropyrrolotriazole, quinolinone, dioxaspirodecane. It will also be understood that when specified, optional substituents on a heterocyclyl group may be present on any substitutable position and, include, e.g., the position at which the heterocyclyl is attached.
As used herein the terms “subject” and “patient” may be used interchangeably, and means a mammal in need of treatment, e.g., companion animals (e.g., dogs, cats, and the like), farm animals (e.g., cows, pigs, horses, sheep, goats and the like) and laboratory animals (e.g., rats, mice, guinea pigs and the like). Typically, the subject is a human in need of treatment.
Certain of the disclosed compounds may exist in various stereoisomeric forms. Stereoisomers are compounds that differ only in their spatial arrangement. Enantiomers are pairs of stereoisomers whose mirror images are not superimposable, most commonly because they contain an asymmetrically substituted carbon atom that acts as a chiral center. “Enantiomer” means one of a pair of molecules that are mirror images of each other and are not superimposable. Diastereomers are stereoisomers that contain two or more asymmetrically substituted carbon atoms. The symbol “*” in a structural formula represents the presence of a chiral carbon center. “R” and “S” represent the configuration of substituents around one or more chiral carbon atoms. Thus, “R*” and “S*” denote the relative configurations of substituents around one or more chiral carbon atoms.
“Racemate” or “racemic mixture” means a compound of equimolar quantities of two enantiomers, wherein such mixtures exhibit no optical activity, i.e., they do not rotate the plane of polarized light.
“Geometric isomer” means isomers that differ in the orientation of substituent atoms in relationship to a carbon-carbon double bond, to a cycloalkyl ring, or to a bridged bicyclic system. Atoms (other than H) on each side of a carbon-carbon double bond may be in an E (substituents are on opposite sides of the carbon-carbon double bond) or Z (substituents are oriented on the same side) configuration. “R,” “S,” “St,” “R*,” “E,” “Z,” “cis,” and “trans,” indicate configurations relative to the core molecule.
The compounds of the invention may be prepared as individual enantiomers by either enantio-specific synthesis or resolved from an enantiomerically enriched mixture. Conventional resolution techniques include forming the salt of a free base of each isomer of an enantiomeric pair using an optically active acid (followed by fractional crystallization and regeneration of the free base), forming the salt of the acid form of each enantiomer of an enantiomeric pair using an optically active amine (followed by fractional crystallization and regeneration of the free acid), forming an ester or amide of each of the enantiomers of an enantiomeric pair using an optically pure acid, amine or alcohol (followed by chromatographic separation and removal of the chiral auxiliary), or resolving an enantiomeric mixture of either a starting material or a final product using various well known chromatographic methods.
When the stereochemistry of a disclosed compound is named or depicted by structure, the named or depicted stereoisomer is at least 60%, 70%, 80%, 90%, 99% or 99.9% by weight pure relative to all of the other stereoisomers. Percent by weight pure relative to all of the other stereoisomers is the ratio of the weight of one stereoisomer over the weight of the the other stereoisomers. When a single enantiomer is named or depicted by structure, the depicted or named enantiomer is at least 60%, 70%, 80%, 90%, 99% or 99.9% by weight optically pure. Percent optical purity by weight is the ratio of the weight of the enantiomer over the weight of the enantiomer plus the weight of its optical isomer.
When a disclosed compound is named or depicted by structure without indicating the stereochemistry, and the compound has one chiral center, it is to be understood that the name or structure encompasses one enantiomer of compound free from the corresponding optical isomer, a racemic mixture of the compound and mixtures enriched in one enantiomer relative to its corresponding optical isomer.
When a disclosed compound is named or depicted by structure without indicating the stereochemistry and e.g., the compound has at least two chiral centers, it is to be understood that the name or structure encompasses one stereoisomer free of other stereoisomers, mixtures of stereoisomers, and mixtures of stereoisomers in which one or more stereoisomers is enriched relative to the other stereoisomer(s). For example, the name or structure may encompass one stereoisomer free of other diastereomers, mixtures of stereoisomers, and mixtures of stereoisomers in which one or more diastereomers is enriched relative to the other diastereomer(s).
The compounds of the invention may be present in the form of pharmaceutically acceptable salts. For use in medicines, the salts of the compounds of the invention refer to nontoxic “pharmaceutically acceptable salts.” Pharmaceutically acceptable salt forms include pharmaceutically acceptable acidic/anionic or basic/cationic salts.
Pharmaceutically acceptable basic/cationic salts include, the sodium, potassium, calcium, magnesium, diethanolamine, n-methyl-D-glucamine, L-lysine, L-arginine, ammonium, ethanolamine, piperazine and triethanolamine salts.
Pharmaceutically acceptable acidic/anionic salts include, e.g., the acetate, benzenesulfonate, benzoate, bicarbonate, bitartrate, carbonate, citrate, dihydrochloride, gluconate, glutamate, glycollylarsanilate, hexylresorcinate, hydrobromide, hydrochloride, malate, maleate, malonate, mesylate, nitrate, salicylate, stearate, succinate, sulfate, tartrate, and tosylate.
3. Description of Exemplary Compounds
In a first embodiment, the present invention provides a compound of Formula (I),




or a pharmaceutically acceptable salt thereof, wherein the variables are as described above.

In a second embodiment, the compound of Formula (I) is of Formula (II):




or a pharmaceutically acceptable salt thereof, wherein the variables in structural Formula (II) are as described for Formula (I).

In a third embodiment, the compound of Formula (I) is of Formula (III):




or a pharmaceutically acceptable salt thereof, wherein the variables in structural Formula (III) are as described for Formula (I).

In a fourth embodiment, the compound of Formula (I) is of Formula (IV):




or a pharmaceutically acceptable salt thereof, wherein the variables in structural Formula (IV) are as described for Formula (I).

In a fifth embodiment, the compound of Formula (I) is of Formula (V):




or a pharmaceutically acceptable salt thereof, wherein the variables in structural Formula (V) are as described for Formula (I).

In a sixth embodiment, the compound of Formula (I) is of Formula (VI):




or a pharmaceutically acceptable salt thereof, wherein the variables in structural Formula (VI) are as described for Formula (I).

In a seventh embodiment, the compound of Formula (I) is of Formula (VII) or (VIIa):




or a pharmaceutically acceptable salt thereof, wherein the variables in structural Formula (VII) and structural Formula (VIIa) are as described for Formula (I).

In an eighth embodiment, R2 in Formulas (I) to (VIIa) is (C1-C3)alkyl, wherein the remainder of the variables are as described in Formula (I).
In a ninth embodiment, Cy2 in Formulas (I) to (VIIa) is selected from aryl, monocyclic cycloalkyl, monocyclic heterocyclyl, and bicyclic heterocyclyl, each of which is optionally substituted with 1 to 2 groups independently selected from R6, wherein the remainder of the variables are as described in Formula (I) or the eighth embodiment.
In a tenth embodiment, Cy2 in Formulas (I) to (VIIa) is phenyl, cyclohexyl, dioxanyl, tetrahydropyranyl or 4,5,6,7-tetrahydrobenzo[d]oxazolyl, each of which is optionally substituted with 1 to 2 groups independently selected from R6, wherein the remainder of the varables are as described in Formula (I) or the eighth or ninth embodiment.
In an eleventh embodiment, Cy1 in Formulas (I) to (VIIa) is phenyl, piperidinyl, or pyridinyl, each of which is optionally substituted with 1 to 2 groups independently selected from R5, wherein the remainder of the varables are as described in Formula (I) or the eighth, ninth, or tenth embodiment.
In a twelfth embodiment, Cy1 in Formulas (I) to (VIIa) is phenyl or pyridinyl, each of which is optionally substituted with 1 to 2 groups independently selected from R5; and Cy2 is cyclohexyl or phenyl, each of which is optionally substituted with 1 to 2 groups independently selected from R6, wherein the remainder of the varables are as described in Formula (I) or the eighth, ninth, tenth, or eleventh embodiment.
In a thirteenth embodiment, R7 in Formulas (I) to (VIIa) is hydrogen; and R8 is hydrogen, hydroxy(C1-C3)alkyl, (C1-C3)alkoxy(C1-C3)alkyl, aminocarbonyl-O(C1-C3)alkyl, hydroxycarbonyl(C1-C3)alkoxy(C1-C3)alkyl, (C1-C3)alkylcarbonylamino(C1-C3)alkyl, (C1-C3)alkoxycarbonylamino(C1-C3)alkyl, di(C1-C3)alkylamino(C1-C3)alkyl, di(C1-C3)alkylaminocarbonyl(C1-C3)alkyl, (C1-C3)alkylaminocarbonyl(C1-C3)alkyl, aminocarbonyl(C1-C3)alkyl, or aminocarbonyl, wherein the remainder of the varables are as described in Formula (I) or the eighth, ninth, tenth, eleventh, or twelfth embodiment. Alternatively, R7 is hydrogen; and R8 is hydrogen, —CH2OH, —(CH2)2OH, —CH2OCH3, —CH2OC(O)NH2, —CH2OCH2COOH, —CH2NHC(O)CH3, —CH2NHC(O)OCH3, —(CH2)2N(CH3)2, —CH2C(O)N(CH3)2, —CH2C(O)NHCH3, —CH2C(O)NH2, or CONH2, wherein the remainder of the varables are as described in Formula (I) or the eighth, ninth, tenth, eleventh, or twelfth embodiment. In another alternative, R7 is hydrogen; and R8 is hydrogen, —CH2OH, —(CH2)2OH, or —CH2OCH3, wherein the remainder of the varables are as described in Formula (I) or the eighth, ninth, tenth, eleventh, or twelfth embodiment.
In a fourteenth embodiment, R5 in Formulas (I) to (VIIa) is selected from halo, (C1-C3)alkyl, halo(C1-C3)alkyl, cyano, hydroxy(C1-C3)alkyl, (C1-C3)alkoxycarbonyl, (C1-C3)alkylsulfonyl, (C1-C3)alkoxy, halo(C1-C3)alkoxy, oxo, hydroxy, (C1-C3)alkylcarbonyl, hydroxy(C1-C3)alkylcarbonyl, (C1-C3)alkylhydroxycarbonyl, (C1-C3)alkylaminosulfonyl, (C1-C3)alkylaminocarbonyl, di(C1-C3)alkylamino(C2-C6)alkoxy, (C1-C6)alkoxycarbonyl, [(C1-C3)alkyl(C4-C6)heterocyclyl](C1-C3)alkyl, and (C1-C3)alkylhydroxy(C1-C3)alkyl; and R6 is selected from halo, (C1-C3)alkyl, halo(C1-C3)alkyl, cyano, hydroxy(C1-C3)alkyl, (C1-C3)alkoxycarbonyl, (C1-C3)alkylsulfonyl, (C1-C3)alkoxy, halo(C1-C3)alkoxy, oxo, hydroxy, aryl(C1-C3)alkoxycarbonyl, (C1-C3)alkylhydroxy(C1-C3)alkyl, heteroaryl, and (C1-C3)alkoxycarbonyl, wherein the remainder of the varables are as described in Formula (I) or the eighth, ninth, tenth, eleventh, twelfth, or thirteenth embodiment. Alternatively, R5 is (C1-C3)alkylsulfonyl, wherein the remainder of the varables are as described in Formula (I) or the eighth, ninth, tenth, eleventh, twelfth, or thirteenth embodiment. In another alternative, R6 is selected from (C1-C3)alkyl, halo(C1-C3)alkyl, hydroxy, and (C1-C3)alkoxy, wherein the remainder of the varables are as described in Formula (I) or the eighth, ninth, tenth, eleventh, twelfth, or thirteenth embodiment. In another alternative, R5 is —SO2CH2CH3 or —SO2CH3, wherein the remainder of the varables are as described in Formula (I) or the eighth, ninth, tenth, eleventh, twelfth, or thirteenth embodiment. In another alternative, R6 is CF3, wherein the remainder of the varables are as described in Formula (I) or the eighth, ninth, tenth, eleventh, twelfth, or thirteenth embodiment.
In a fifteenth embodiment, R2 in Formulas (I) to (VIIa) is ethyl or isopropyl, wherein the remainder of the varables are as described in Formula (I) or the eighth, ninth, tenth, eleventh, twelfth, thirteenth, or fourteenth embodiment.
In a sixteenth embodiment, the compound of Formula (I) is of Formula (VIII):




or a pharmaceutically acceptable salt thereof, wherein

X is CH or N;
Y1 is O and Y2 and Y3 are each CH2, Y1 and Y3 are each CH2 and Y2 is O, Y1 and Y3 are each O and Y2 is CH2, Y1 and Y2 are each O and Y3 is CH2, or Y1, Y2 and Y3 are each CH2;
R2 is (C1-C3)alkyl;
R4 is hydrogen, (C1-C3)alkyl, or ═O;
R7 and R8 are each independently selected from hydrogen, (C1-C3)alkyl, (C1-C3)alkoxy(C1-C3)alkyl, halo(C1-C3)alkyl, hydroxy(C1-C3)alkyl, (C1-C3)alkoxy, aminocarbonyl-O(C1-C3)alkyl, (C1-C3)alkyl-aminocarbonyl-O(C1-C3)alkyl, and di(C1-C3)alkyl-aminocarbonyl-O(C1-C3)alkyl;
each R6 is independently selected from halo(C1-C3)alkyl, (C1-C3)alkoxy, and hydroxy; and
R5 is (C1-C3)alkylsulfonyl.
In a seventeenth embodiment, the compound of Formula (I) is of Formula (IX):




or a pharmaceutically acceptable salt thereof, wherein the variables in structural Formula (IX) are as described for Formula (VIII).

In an eighteenth embodiment, the compound of Formula (I) is of Formula (X):




or a pharmaceutically acceptable salt thereof, wherein the variables in structural Formula (X) are as described for Formula (VIII).

In a nineteenth embodiment, R4 in compounds of Formulas (VIII) to (X) is hydrogen or ═O; and R8 is hydrogen, hydroxy(C1-C3)alkyl, (C1-C3)alkoxyl(C1-C3)alkyl, or aminocarbonyl-O(C1-C3)alkyl, wherein the remaining variables are as described in Formula (VIII).
In a twentieth embodiment, the compound of Formula (I) is of Formula (XI):




or a pharmaceutically acceptable salt thereof, wherein the variables in structural Formula (XI) are as described for Formula (VIII) or the nineteenth embodiment.

In a twenty-first embodiment, the compound of Formula (I) is of Formula (XII):




or a pharmaceutically acceptable salt thereof, wherein the variables in structural Formula (XII) are as described for Formula (VIII) or the nineteenth embodiment.

In a twenty-second embodiment, R6 in compounds of Formulas (VIII) to (XII) is CF3; and R5 is —SO2(C1-C3)alkyl, wherein the remaining variables are as described for Formula (VIII) or the nineteenth embodiment. Alternatively, R6 in compounds of Formulas (VIII) to (XII) is CF3; R5 is —SO2(C1-C3)alkyl and X is N, wherein the remaining variables are as described for Formula (VIII) or the nineteenth embodiment
In a twenty-third embodiment, the compound of Formula (I) is of Formula (XIII)




or a pharmaceutically acceptable salt thereof, wherein each R6 is independently selected from halo(C1-C3)alkyl, (C1-C3)alkoxy, and hydroxy; and R5 is (C1-C3)alkylsulfonyl, wherein the remaining variables are as described in Formula (I).

In a twenty-fourth embodiment, the compound of Formula (I) is of Formula (XIV):




or a pharmaceutically acceptable salt thereof, wherein the variables in structural Formula (XIV) are as described in Formula (I) or the twenty-third embodiment.

In a twenty-fifth embodiment, the compound of Formula (I) is of Formula (XV):




or a pharmaceutically acceptable salt thereof, wherein the variables in structural Formula (XV) are as described in Formula (I) or the twenty-third embodiment.

In a twenty-sixth embodiment, the compound of Formula (I) is of Formula (XVI):




or a pharmaceutically acceptable salt thereof, wherein the variables in structural Formula (XVI) are as described in Formula (I) or the twenty-third embodiment.

In a twenty-seventh embodiment, the compound of Formula (I) is of Formula (XVII):




or a pharmaceutically acceptable salt thereof, wherein the variables in structural Formula (XVII) are as described in Formula (I) or the twenty-third embodiment.

In a twenty-eighth embodiment, the compound of Formula (I) is of Formula (XVIII) or (XIX):




or a pharmaceutically acceptable salt thereof, wherein the variables in structural Formulas (XVIII) and (XIX) are as described in Formula (I) and the twenty-third embodiment.

In a twenty-ninth embodiment, R2 in Formulas (XIII) to (XIX) is (C1-C3)alkyl, wherein the remainder of the variables are as described in Formula (I) or the twenty-third embodiment.
In a thirtieth embodiment, Cy2 in Formulas (XIII) to (XIX) is selected from aryl, monocyclic cycloalkyl, monocyclic heterocyclyl, and bicyclic heterocyclyl, wherein the remainder of the variables are as described in Formula (I) or the twenty-third or twenty-ninth embodiment.
In a thirty-first embodiment, Cy2 in Formulas (XIII) to (XIX) is phenyl, cyclohexyl, or 4,5,6,7-tetrahydrobenzo[d]oxazolyl, wherein the remainder of the variables are as described in Formula (I) or the twenty-third, twenty-ninth, or thirtieth embodiment.
In a thirty-second embodiment, Cy1 in Formulas (XIII) to (XIX) is phenyl, piperidinyl, or pyridinyl, wherein the remainder of the variables are as described in Formula (I) or the twenty-third, twenty-ninth, thirtieth, or thirty-first embodiment.
In a thirty-third embodiment, Cy1 in Formulas (XIII) to (XIX) is phenyl or pyridinyl; and Cy2 is cyclohexyl or phenyl, wherein the remainder of the variables are as described in Formula (I) or the twenty-third, twenty-ninth, thirtieth, thirty-first, or thirty-second embodiment.
In a thirty-fourth embodiment, R7 in Formulas (XIII) to (XIX) is hydrogen; and R8 is hydrogen, hydroxy(C1-C3)alkyl, (C1-C3)alkoxy(C1-C3)alkyl, aminocarbonyl-O(C1-C3)alkyl, hydroxycarbonyl(C1-C3)alkoxy(C1-C3)alkyl, (C1-C3)alkylcarbonylamino(C1-C3)alkyl, (C1-C3)alkoxycarbonylamino(C1-C3)alkyl, di(C1-C3)alkylamino(C1-C3)alkyl, di(C1-C3)alkylaminocarbonyl(C1-C3)alkyl, (C1-C3)alkylaminocarbonyl(C1-C3)alkyl, aminocarbonyl(C1-C3)alkyl, or aminocarbonyl, wherein the remainder of the variables are as described in Formula (I) or the twenty-third, twenty-ninth, thirtieth, thirty-first, or thirty-second, or thirty-third embodiment.
In a thirty-fifth embodiment, R7 in Formulas (XIII) to (XIX) is hydrogen; and R8 is hydrogen, —CH2OH, —(CH2)2OH, —CH2OCH3, —CH2OC(O)NH2, —CH2OCH2COOH, —CH2NHC(O)CH3, —CH2NHC(O)OCH3, —(CH2)2N(CH3)2, —CH2C(O)N(CH3)2, —CH2C(O)NHCH3, —CH2C(O)NH2, or CONH2, wherein the remainder of the variables are as described in Formula (I) or the twenty-third, twenty-ninth, thirtieth, thirty-first, or thirty-second, thirty-third, or thirty-fourth embodiment.
In a thirty-sixth embodiment, R7 in Formulas (XIII) to (XIX) is hydrogen; and R8 is hydrogen, —CH2OH, —(CH2)2OH, or —CH2OCH3, wherein the remainder of the variables are as described in Formula (I) or the twenty-third, twenty-ninth, thirtieth, thirty-first, or thirty-second, thirty-third, thirty-fourth, or thirty-fifth embodiment.
In a thirty-seventh embodiment, R5 in Formulas (XIII) to (XIX) is —SO2CH2CH3 or —SO2CH3; and R6 is CF3, wherein the remainder of the variables are as described in Formula (I) or the twenty-third, twenty-ninth, thirtieth, thirty-first, or thirty-second, thirty-third, thirty-fourth, thirty-fifth, or thirty-sixth embodiment.
Specific examples of compounds of the invention are provided in the EXEMPLIFICATION. Pharmaceutically acceptable salts as well as the neutral forms of these compounds are included in the invention.
In certain embodiments, the present invention provides any one of the compounds in the foregoing examples, or a pharmaceutically acceptable salt thereof.
In certain embodiments, the present invention provides a method of treating a patient (e.g., a human) with a disorder mediated by RORγ comprising the step of administering to the patient an effective amount of the compound with any compound described herein, or a pharmaceutically acceptable salt or composition thereof.
4. Uses, Formulation and Administration
Pharmaceutically Acceptable Compositions
According to another embodiment, the present invention provides a method of treating a subject (e.g., a human) with a disorder mediated by RORγ using a composition comprising a compound of the invention and a pharmaceutically acceptable carrier, adjuvant, or vehicle. In certain embodiments, the amount of compound of the invention in a provided composition is such that it is effective as an inverse agonist or antagonist to RORγ in a biological sample or in a subject. In certain embodiments, a provided composition is formulated for administration to a subject in need of such composition. In some embodiments, a provided composition is formulated for oral administration to a subject.
The term “pharmaceutically acceptable carrier, adjuvant, or vehicle” refers to a nontoxic carrier, adjuvant, or vehicle that does not destroy the pharmacological activity of the compound with which it is formulated. Pharmaceutically acceptable carriers, adjuvants or vehicles that may be used in the compositions of this disclosure include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins, such as human serum albumin, buffer substances such as phosphates, glycine, sorbic acid, potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, cellulose-based substances, polyethylene glycol, sodium carboxymethylcellulose, polyacrylates, waxes, polyethylene-polyoxypropylene-block polymers, polyethylene glycol and wool fat.
Compositions described herein may be administered orally, parenterally, by inhalation spray, topically, rectally, nasally, buccally, vaginally or via an implanted reservoir. The term “parenteral” as used herein includes subcutaneous, intravenous, intramuscular, intra-articular, intra-synovial, intrasternal, intrathecal, intrahepatic, intralesional and intracranial injection or infusion techniques.
Liquid dosage forms for oral administration include, but are not limited to, pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs. In addition to the active compounds, the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof. Besides inert diluents, the oral compositions can also include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents.
Injectable preparations, for example, sterile injectable aqueous or oleaginous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution, suspension or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution, U.S.P. and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil can be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid are used in the preparation of injectables.
Injectable formulations can be sterilized, for example, by filtration through a bacterial-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use.
In order to prolong the effect of a provided compound, it is often desirable to slow the absorption of the compound from subcutaneous or intramuscular injection. This may be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of the compound then depends upon its rate of dissolution that, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered compound form is accomplished by dissolving or suspending the compound in an oil vehicle. Injectable depot forms are made by forming microencapsule matrices of the compound in biodegradable polymers such as polylactide-polyglycolide. Depending upon the ratio of compound to polymer and the nature of the particular polymer employed, the rate of compound release can be controlled. Examples of other biodegradable polymers include poly(orthoesters) and poly(anhydrides). Depot injectable formulations are also prepared by entrapping the compound in liposomes or microemulsions that are compatible with body tissues.
Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active compound is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffin, f) absorption accelerators such as quaternary ammonium compounds, g) wetting agents such as, for example, cetyl alcohol and glycerol monostearate, h) absorbents such as kaolin and bentonite clay, and i) lubricants such as talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, and mixtures thereof. In the case of capsules, tablets and pills, the dosage form may also comprise buffering agents.
Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical formulating art. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes. Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like.
Provided compounds can also be in micro-encapsulated form with one or more excipients as noted above. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings, release controlling coatings and other coatings well known in the pharmaceutical formulating art. In such solid dosage forms the active compound may be admixed with at least one inert diluent such as sucrose, lactose or starch. Such dosage forms may also comprise, as is normal practice, additional substances other than inert diluents, e.g., tableting lubricants and other tableting aids such a magnesium stearate and microcrystalline cellulose. In the case of capsules, tablets and pills, the dosage forms may also comprise buffering agents. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes.
Dosage forms for topical or transdermal administration of a compound of this invention include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants or patches. The active component is admixed under sterile conditions with a pharmaceutically acceptable carrier and any needed preservatives or buffers as may be required. Ophthalmic formulation, ear drops, and eye drops are also contemplated as being within the scope of this invention. Additionally, the present invention contemplates the use of transdermal patches, which have the added advantage of providing controlled delivery of a compound to the body. Such dosage forms can be made by dissolving or dispensing the compound in the proper medium. Absorption enhancers can also be used to increase the flux of the compound across the skin. The rate can be controlled by either providing a rate controlling membrane or by dispersing the compound in a polymer matrix or gel.
Pharmaceutically acceptable compositions provided herein may be formulated for oral administration. Such formulations may be administered with or without food. In some embodiments, pharmaceutically acceptable compositions of this disclosure are administered without food. In other embodiments, pharmaceutically acceptable compositions of this disclosure are administered with food.
The amount of provided compounds that may be combined with carrier materials to produce a composition in a single dosage form will vary depending upon the patient to be treated and the particular mode of administration.
It should also be understood that a specific dosage and treatment regimen for any particular patient will depend upon a variety of factors, including age, body weight, general health, sex, diet, time of administration, rate of excretion, drug combination, the judgment of the treating physician, and the severity of the particular disease being treated. The amount of a provided compound in the composition will also depend upon the particular compound in the composition.
Uses of Compounds and Pharmaceutically Acceptable Compositions
Compounds and compositions described herein are generally useful for the inhibition of RORγ. Thus, in some embodiments, the present invention provides a method of treating inflammatory, metabolic and autoimmune diseases or disorders mediated by RORγ, comprising administering a provided compound or composition. More particularly, the compounds and compositions described herein act as inverse agonists or antagonists of RORγ.
As used herein, the terms “treatment,” “treat,” and “treating” refer to reversing, alleviating, delaying the onset of, or inhibiting the progress of a disease or disorder, or one or more symptoms thereof, as described herein. In some embodiments, treatment may be administered after one or more symptoms have developed, i.e., therapeutic treatment. In other embodiments, treatment may be administered in the absence of symptoms. For example, treatment may be administered to a susceptible individual prior to the onset of symptoms (e.g., in light of a history of symptoms and/or in light of genetic or other susceptibility factors), i.e., prophylactic treatment. Treatment may also be continued after symptoms have resolved, for example to prevent or delay their recurrence.
Diseases and conditions treatable according to the methods of the invention include, but are not limited to, inflammatory, metabolic and autoimmune diseases or disorders mediated by RORγ. These diseases and conditions include, for example, asthma, chronic obstructive pulmonary disease (COPD), bronchitis, allergic rhinitis, atopic dermatitis, contact dermatitis, acne, urticaria, hives, angioedema, cystic fibrosis, allograft rejection, multiple sclerosis, Balo's concentric (circular) sclerosis, Balo disease, leukoencephalitis periaxialis concentrica, encephalitis periaxialis concentrica, scleroderma, limited scleroderma, CREST syndrome, arthritis, rheumatoid arthritis, juvenile rheumatoid arthritis, reactive arthritis, Reiter's syndrome, osteoarthritis, ankylosing spondylitis, systemic lupus erythematosus (SLE), psoriasis, plaque psoriasis, guttate psoriasis, inverse psoriasis, pustular psoriasis, erythrodermic psoriasis, psoriatic epidermal hyperplasia, epidermal hyperplasia, Hashimoto's disease, pancreatitis, autoimmune diabetes, type I diabetes, autoimmune ocular disease, ulcerative colitis, Crohn's disease, regional enteritis, inflammatory bowel disease (IBD), inflammatory bowel syndrome (IBS), Sjögren's syndrome, optic neuritis, obesity, hepatosteatosis, adipose tissue-associated inflammation, insulin resistance, type II diabetes, neuromyelitis optica, myasthenia gravis, age related macular degeneration, dry eye, uveitis, Guillain-Barré syndrome, psoriasis, psoriatic arthritis (PsA), steroid resistant asthma, Graves' disease, scleritis, endometriosis, obstructive sleep apnea syndrome (OSAS), Behçet's disease, dermatomyositis, polymyositis, graft versus host disease, chronic graft versus host disease, acute graft versus host disease, primary biliary cirrhosis, liver fibrosis, non-alcoholic fatty liver disease (NAFLD), sarcoidosis, primary sclerosing cholangitis, autoimmune thyroid disease, autoimmune polyendocrine syndrome type I, autoimmune polyendocrine syndrome type II, celiac disease, celiac sprue, neuromyelitis, juvenile idiopathic arthritis, systemic sclerosis, myocardial infarction, pulmonary hypertension, osteoarthritis, cutaneous leishmaniasis, sinonasal polyposis, cancer, including but not limited to lung cancer, gastric cancer, breast cancer and colon cancer, thrombocytopenic purpura, idiopathic thrombocytopenic purpura (ITP), immune thrombocytopenic purpura, cartilage inflammation, bone degradation, vasculitis, acute disseminated encephalomyelitis (ADEM), acute necrotizing hemorrhagic leukoencephalitis, Addison's disease, agammaglobulinemia, alopecia areata, amyloidosis, anti-glomerular basement membrane (GBM) nephritis, anti-tubular basement membrane (TBM) nephritis, antiphospholipid syndrome (APS), autoimmune angioedema, autoimmune aplastic anemia, autoimmune dysautonomia, autoimmune hepatitis, autoimmune hyperlipidemia, autoimmune immunodeficiency, autoimmune inner ear disease (AIED), autoimmune myocarditis, autoimmune oophoritis, autoimmune pancreatitis, autoimmune retinopathy, autoimmune thrombocytopenic purpura (ATP), autoimmune thyroid disease, autoimmune urticaria, axonal and neuronal neuropathies, bullous pemphigoid, cardiomyopathy, Castleman disease, Chagas disease, chronic inflammatory demyelinating polyneuropathy (CIDP), chronic recurrent multifocal ostomyelitis (CRMO), Churg-Strauss syndrome, cicatricial pemphigoid, benign mucosal pemphigoid, Cogan's syndrome, cold agglutinin disease, congenital heart block, coxsackie myocarditis, essential mixed cryoglobulinemia, demyelinating neuropathies, dermatitis herpetiformis, Devic's disease, neuromyelitis optica, discoid lupus, Dressler's syndrome, eosinophilic esophagitis, eosinophilic fasciitis, erythema nodosum, experimental allergic encephalomyelitis, Evans syndrome, fibrosing alveolitis, giant cell arteritis, temporal arteritis, giant cell myocarditis, glomerulonephritis, Goodpasture's syndrome, granulomatosis with polyangiitis (GPA), Wegener's granulomatosis, Hashimoto's encephalitis, Hashimoto's thyroiditis, hemolytic anemia, Henoch-Schonlein purpura, herpes gestationis, hypogammaglobulinemia, IgA nephropathy, IgG4-related sclerosing disease, immunoregulatory lipoproteins, inclusion body myositis, interstitial cystitis, juvenile myositis, Kawasaki syndrome, Lambert-Eaton syndrome, leukocytoclastic vasculitis, lichen planus, lichen sclerosus, ligneous conjunctivitis, linear IgA disease (LAD), chronic Lyme disease, Meniere's disease, microscopic polyangiitis, mixed connective tissue disease (MCTD), Mooren's ulcer, Mucha-Habermann disease, myositis, narcolepsy, neuromyelitis optica, Devic's syndrome, neutropenia, ocular cicatricial pemphigoid, optic neuritis, palindromic rheumatism, pediatric autoimmune neuropsychiatric disorders associated with streptococcus (PANDAS), paraneoplastic cerebellar degeneration, paroxysmal nocturnal hemoglobinuria (PNH), Parry-Romberg syndrome, Parsonnage-Turner syndrome, pars planitis, peripheral uveitis, pemphigus, peripheral neuropathy, perivenous encephalomyelitis, pernicious anemia, POEMS syndrome, polyarteritis nodosa, type I autoimmune polyglandular syndrome, type II autoimmune polyglandular syndrome, type III autoimmune polyglandular syndrome, polymyalgia rheumatic, postmyocardial infarction syndrome, postpericardiotomy syndrome, progesterone dermatitis, primary biliary cirrhosis, primary sclerosing cholangitis, idiopathic pulmonary fibrosis, pyoderma gangrenosum, pure red cell aplasia, Raynaud's phenomenon, reflex sympathetic dystrophy, relapsing polychondritis, restless legs syndrome, retroperitoneal fibrosis, rheumatic fever, rheumatoid arthritis, sarcoidosis, Schmidt syndrome, sperm autoimmunity, stiff person syndrome, subacute bacterial endocarditis (SBE), Susac's syndrome, sympathetic ophthalmia, Takayasu's arteritis, temporal arteritis, giant cell arteritistesticular autoimmunity, Tolosa-Hunt syndrome, transverse myelitis, undifferentiated connective tissue disease (UCTD), vesiculobullous dermatosis, and vitiligo.
Also included are diseases or disorders which are implicated by the regulation of the circadian rhythm of individuals and include, e.g., major depression, seasonal affective disorder, post-traumatic stress disorder (PTSD), bipolar disorder, autism, epilepsy, Alzheimer's and other central nervous system (CNS) disorders associated with altered sleep and/or circadian rhythms.
In one embodiment, a human patient is treated with a compound of the invention and a pharmaceutically acceptable carrier, adjuvant, or vehicle, wherein said compound is present in an amount to treat or ameliorate one or more of the diseases and conditions recited above. In another embodiment, the diseases and conditions treated or ameliorated by a compound of the invention include, e.g., asthma, COPD, bronchitis, allergic rhinitis, atopic dermatitis, contact dermatitis, acne, urticaria, cystic fibrosis, allograft rejection, multiple sclerosis, scleroderma, arthritis, rheumatoid arthritis, juvenile rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, SLE, psoriasis, Hashimoto's disease, pancreatitis, autoimmune diabetes, type I diabetes, autoimmune ocular disease, ulcerative colitis, Crohn's disease, regional enteritis, IBD, IBS, Sjögren's syndrome, optic neuritis, obesity, hepatosteatosis, adipose tissue-associated inflammation, insulin resistance, type II diabetes, neuromyelitis optica, myasthenia gravis, age related macular degeneration, dry eye, uveitis, Guillain-Barré syndrome, psoriasis, PsA, steroid resistant asthma, Graves' disease, scleritis, major depression, seasonal affective disorder, PTSD, bipolar disorder, autism, epilepsy, Alzheimer's, CNS disorders associated with altered sleep and/or circadian rhythms, endometriosis, OSAS, Behçet's disease, dermatomyositis, polymyocitis, graft versus host disease, primary biliary cirrhosis, liver fibrosis, NAFLD, sarcoidosis, primary sclerosing cholangitis, autoimmune thyroid disease, autoimmune polyendocrine syndrome type I, autoimmune polyendocrine syndrome type II, celiac disease, neuromyelitis, juvenile idiopathic arthritis, systemic sclerosis, myocardial infarction, pulmonary hypertension, osteoarthritis, cutaneous leishmaniasis, sinonasal polyposis, and cancer. In an alternative embodiment, the diseases and conditions treated or ameliorated by a compound of the invention include, e.g., asthma, atopic dermatitis, acne, Crohn's disease, regional enteritis, ulcerative colitis, Sjögren's syndrome, uveitis, Behçet's disease, dermatomyositis, multiple sclerosis, ankylosing spondylitis, SLE, scleroderma, psoriasis, PsA, steroid resistant asthma and rheumatoid arthritis in the patient.
The invention further relates to a combination therapy for treating or ameliorating a disease or a disorder described herein. In some embodiments, the combination therapy comprises administering at least one compound of the invention in combination with one or more agents for treating or ameliorating inflammatory, metabolic and autoimmune diseases or disorders mediated by RORγ. In some embodiments, the combination therapy comprises administering at least one compound of the invention in combination with one or more agents for treating or ameliorating a disease or a disorder described herein. In some embodiments, the combination therapy comprises administering at least one compound of the invention in combination with one or more agents for the treatment of diseases including asthma, atopic dermatitis, acne, Crohn's disease, regional enteritis, ulcerative colitis, Sjögren's syndrome, uveitis, Behçet's disease, dermatomyositis, multiple sclerosis, ankylosing spondylitis, SLE, scleroderma, psoriasis, PsA, steroid resistant asthma and rheumatoid arthritis.
The compounds according to the invention may also be used in combination with immunotherapies for the treatment of a disease or disorder disclosed herein.
Combination therapy includes, e.g., co-administration of a compound of the invention and one or more other agents, sequential administration of a compound of the invention and one or more other agents, administration of a composition containing a compound of the invention and one or more other agents, or simultaneous administration of separate compositions containing a compound of the invention and one or more other agents.
The invention further provides a method of treating a subject, such as a human, suffering from one of the abovementioned disorders or diseases.
The invention further relates to the use of provided compounds for the production of pharmaceutical compositions which are employed for the treatment and/or prophylaxis and/or amelioration of the diseases and disorders mentioned herein.
Compounds or compositions described herein may be administered using any amount and any route of administration effective for treating or lessening the severity of one or more of the diseases and conditions described herein. The exact amount required will vary from subject to subject, depending on the species, age, and general condition of the subject, the severity of the infection, the particular agent, its mode of administration, and the like. Provided compounds are preferably formulated in unit dosage form for ease of administration and uniformity of dosage. The expression “unit dosage form” as used herein refers to a physically discrete unit of agent appropriate for the patient to be treated. It will be understood, however, that the total daily usage of the compounds and compositions of the present disclosure will be decided by the attending physician within the scope of sound medical judgment. The specific effective dose level for any particular patient or organism will depend upon a variety of factors including the disorder being treated and the severity of the disorder; the activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex and diet of the patient; the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidental with the specific compound employed, and like factors well known in the medical arts.
Pharmaceutically acceptable compositions of this disclosure can be administered to humans and other animals orally, rectally, parenterally, intracisternally, intravaginally, intraperitoneally, topically (as by powders, ointments, or drops), bucally, as an oral or nasal spray, or the like, depending on the severity of the infection being treated. In certain embodiments, provided compounds may be administered orally or parenterally at dosage levels of about 0.01 mg/kg to about 50 mg/kg and preferably from about 1 mg/kg to about 25 mg/kg, of subject body weight per day, one or more times a day, to obtain the desired therapeutic effect.
The term “biological sample”, as used herein, includes, without limitation, cell cultures or extracts thereof, biopsied material obtained from a mammal or extracts thereof, and blood, saliva, urine, feces, semen, tears, or other body fluids or extracts thereof.
The amount of both, a provided compound and additional therapeutic agent (in those compositions which comprise an additional therapeutic agent as described above) that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated and the particular mode of administration.
In those compositions which comprise an additional therapeutic agent, that additional therapeutic agent and the provided compound may act synergistically. Therefore, the amount of additional therapeutic agent in such compositions will be less than that required in a monotherapy utilizing only that therapeutic agent.
The amount of additional therapeutic agent present in the compositions of this disclosure will be no more than the amount that would normally be administered in a composition comprising that therapeutic agent as the only active agent.
EXEMPLIFICATION
As depicted in the Examples below, in certain exemplary embodiments, compounds are prepared according to the following general procedures. It will be appreciated that, although the general methods depict the synthesis of certain compounds of the present invention, the following general methods, and other methods known to one of ordinary skill in the art, can be applied to all compounds and subclasses and species of each of these compounds, as described herein.
General Description of Synthesis
The compounds of the present invention can be readily prepared according to the following reaction schemes and examples, or modifications thereof, using readily available starting materials, reagents and conventional synthesis procedures. Many of the reactions can also be carried out under microwave (MW) conditions or using conventional heating or utilizing other technologies such as solid phase reagents/scavengers or flow chemistry. In these reactions, it is also possible to make use of variants which are themselves known to those of ordinary skill in the art, but are not mentioned in greater detail. Furthermore, other methods for preparing compounds of the invention will be readily apparent to a person of ordinary skill in the art in light of the following reaction schemes and examples. In cases where synthetic intermediates and final products contain potentially reactive functional groups, for example amino, hydroxy, thiol and carboxylic acid groups, that may interfere with the desired reaction, it may be advantageous to employ protected forms of the intermediate. Methods for the selection, introduction and subsequent removal of protecting groups are well known to those skilled in the art. In the discussion below variables have the meanings indicated above unless otherwise indicated. The abbreviations used in these experimental details are listed below and additional ones should be known to a person skilled in the art of synthesis. In addition, one can refer to the following references for suitable methods of synthesis as described in March, Advanced Organic Chemistry, 3rd edition, John Wiley & Sons, 1985, Greene and Wuts, Protective Groups in Organic Synthesis, 2nd edition, John Wiley & Sons, 1991, and Richard Larock, Comprehensive Organic Transformations, 4th edition, VCH publishers Inc., 1989.
Generally, reagents in the reaction schemes are used in equimolar amounts; however, in certain cases it may be desirable to use an excess of one reagent to drive a reaction to completion. This is especially the case when the excess reagent can be readily removed by evaporation or extraction. Bases employed to neutralize HCl in reaction mixtures are generally used in slight to substantial excess (1.05-5 equivalents).
Compounds were purified by various HPLC methods including those described below. Purification techniques, include HPLC methods, are know to those skilled in the art.
TFA Preparative HPLC Method A:
Mobile phase A: water with 0.1% TFA; Mobile phase B: CH3CN; Flow rate: 90 mL/min; Detection: UV 220 nm/254 nm; Column: Phenomenex Synergi Max-RP C18 250*50 mm*10 μm; Column temperature: 30° C.












Time in min
% A
% B

















0.00
80
20


30.00
50
50


30.20
0
100


40.00
0
100









Basic Preparative HPLC Method B:

Mobile phase A: water with 0.05% NH3; Mobile phase B: CH3CN; Flow rate: 30 mL/min; Detection: UV 220 nm/254 nm; Column: Synergi 200 mm×25 mm×5 μm; Column temperature: 30° C.












Time in min
% A
% B

















0.0
63
37


8.0
33
67


8.1
0
100


10.1
0
100


10.1
70
30


12
70
30









Basic Preparative HPLC Method C:

Mobile phase A: water with 0.05% NH3; Mobile phase B: CH3CN; Flow rate: 30 mL/min; Detection: UV 220 nm/254 nm; Column: Xyridge 200*25 mm*5 μm; Column temperature: 30° C.












Time in min
% A
% B

















0.00
99
1


8.00
70
30


8.20
0
100


10.00
0
100









HCl Preparative HPLC Method D:

Mobile phase A: water with 0.05% HCl; Mobile phase B: CH3CN; Flow rate: 25 mL/min; Detection: UV 220 nm/254 nm; Column: Synergi Max-RP 150*30 mm*4 μm; Column temperature: 30° C.












Time in min
% A
% B

















0.00
75
25


12.00
45
55


12.20
0
100


14.00
0
100









HCl Preparative HPLC Method E:

Mobile phase A: water with 0.1% HCl; Mobile phase B: CH3CN; Flow rate: 25 mL/min; Detection: UV 220 nm/254 nm; Column: Agella Venusil ASB C18 150*21.2 mm*5 μm; Column temperature: 40° C.












Time in min
% A
% B

















0.00
80
20


10.00
50
50


10.20
0
100


14.00
0
100









HCl Preparative HPLC Method F:

Mobile phase A: water with 0.05% HCl; Mobile phase B: CH3CN; Flow rate: 25 mL/min; Detection: UV 220 nm/254 nm; Column: Agella Venusil ASB C18 150*21.2 mm*5 μm; Column temperature: 40° C.












Time in min
% A
% B

















0.00
80
20


10.00
50
50


10.20
0
100


14.00
0
100









HCl Preparative HPLC Method G:

Mobile phase A: water with 0.05% HCl; Mobile phase B: CH3CN; Flow rate: 25 mL/min; Detection: UV 220 nm/254 nm; Column: Agella Venusil ASB C18 150*21.2 mm*5 μm; Column temperature: 30° C.












Time in min
% A
% B

















0.00
80
20


8.00
50
50


8.20
0
100


10.00
0
100









HCl Preparative HPLC Method H:

Mobile phase A: water with 0.05% HCl; Mobile phase B: CH3CN; Flow rate: 15 mL/min; Detection: UV 220 nm/254 nm; Column: Gemini 150*25 mm*5 μm; Column temperature: 30° C.












Time in min
% A
% B

















0.00
85
15


8.00
55
45


8.20
0
100


10.00
0
100









Where NMR data are presented, spectra were obtained on a Varian 400 (400 MHz) or 300 (300 MHz) and are reported as ppm downfield from tetramethylsilane with number of proton, multiplicities and coupling constants indicated parenthetically along with reference to deuterated solvent.
The invention is illustrated by way of the following examples, in which the following abbreviations may be employed.











Abbreviation
Meaning







ACN, MeCN, CH3CN
acetonitrile


AIBN
azobisisobutyronitrile


aq
aqueous


Boc
tert-butoxycarbonyl or t-butoxycarbonyl


brine
saturated aqueous NaCl


Cbz
benzyloxy carbonyl


Cpd
compound


DCM or CH2Cl2
methylene chloride


DIEA
diisopropyl ethyl amine


DMF
dimethyl formamide


DMS/Me2S
dimethyl sulfide


DMSO
dimethyl sulfoxide


EDCI
1-(3-dimethylaminopropyl)-3-ethylcarbodiiimide



hydrochloride


EtI
ethyl iodide


Et
ethyl


Et2O
ethyl ether


Et3SiH
triethylsilane


Et3N
triethylamine


EtOAc, EA, AcOEt
ethyl acetate


EtOH
ethanol


h, hr
hour(s)


HATU
O-(7-azabenzotriazol-1-yl)-N,N,N′,N′-



tetramethyluronium-hexafluorophosphate


HBTU
O-benzotriazole-1-yl-N,N,N′,N′-



tetramethyluronium-hexafluorophosphate


HCl
hydrochloric acid


H2O2
hydrogen peroxide


HPLC
high performance liquid chromatography


i-BuOCOCl
iso-butoxycarbonyl chloride


ICl
iodochloride


K3PO4
tripotassium phosphate


LC-MS
liquid chromatography-mass spectrometry


LDA
lithium diiisopropylamide


MCPBA, m-CPBA
meta-chloroperoxybenzoic acid


MeOH
methanol


MeI
methyl iodide


Me
methyl


mg
milligram


min
minute(s)


mL
milliliters


mmol
millimoles


mp, m.p.
melting point


MS
mass spectrometry


MW, uwave
microwave


NBS
N-bromosuccinimide


n-BuLi
n-butyllithium


NMM
N-methyl-morpholine


NMP
N-methyl-pyrrolidin-2-one


OTf
trifluoromethanesulfonate


OTs
tosylate


PdCl2dppf
[1,1-bis(diphenylphosphino)ferrocene]



dichloropalladium(ii)


Pd2(dba)3
tris(dibenzylideneacetone)dipalladium(0)


PE
petroleum ether


rt
room temperature


sat.
saturated


SFC
supercritical fluid chromatography


t-BuOK
potassium tert butoxide


t-BuLi
tert butyl lithium


t-BuOOH
tert butyl peroxide


TBAF
tetrabutylammonium fluoride


TFA
trifluoroacetic acid


THF
tetrahydrofuran


TLC
thin layer chromatography


Ti(OEt)4
titanium tetra ethoxide









Compounds according to Formula (I), can be prepared by reacting an intermediate compound of Formula (500) with an alkyl, arylalkyl, heteroaryl or aryl halide, according to reaction Scheme 1, a reaction that is performed in a polar aprotic solvent, such as, for example, acetonitrile, in the presence of a suitable base, such as, for example, N,N-diisopropylethylamine or potassium carbonate. Alternatively, the final compounds according to Formula (I) in which m=1 or 2, can be prepared by reacting an intermediate compound of Formula (500) with an aldehyde, according to reaction Scheme 1, following art-known reductive amination procedure, in the typical solvent, such as, for example, dichloroethane, dichloromethane, or methanol; in the presence of suitable reducing reagent, such as sodium cyanoborohydride or sodium triacetoxyborohydride. In reaction Scheme 1, all variables are defined as in Formula (I) and G1 is a leaving group, such as for example, bromide, chloride, mesylate (methanesulfonate), tosylate (p-toluenesulfonate), trifluorormethanesulfonate (triflate), or iodide.




Intermediate compound of Formula (500) can be can be prepared by deprotecting an intermediate compound of Formula (501), wherein Pg is a suitable nitrogen protecting group (Greene and Wuts, Protective Groups in Organic Synthesis, 2nd edition, John Wiley & Sons, 1991), e.g., Pg=tert-butoxycarbonyl, removed with trifluoroacetic acid according to Scheme 2. In reaction Scheme 2, all variables are defined as in Formula (I).




Intermediate compound of Formula (502), wherein X is C(═O)NH, can be prepared from a carboxylic acid (504) and an amine (503), according to Scheme 3. The reaction is conveniently carried out in the presence of an activating reagent, for example, N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDCI) or O-(7-azabenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HATU), in an organic solvent, for example, N,N-dimethylformamide or dichloromethane, optionally in the presence of a base, e.g., N,N-diisopropylethylamine or triethylamine, at a temperature, for example in the range from 0 to 60° C.




Intermediate compound of Formula (505), wherein X is NHC═O, can be prepared from an intermediate compound of Formula (506) and an amide (507), according to Scheme 4. The reaction is carried out in the presence of a catalyst, for example, tris(dibenzylideneacetone)dipalladium (0) (Pd2(dba)3), in an organic solvent, for example, dioxane or tert-butanol, in the presence of an additive, e.g., potassium phosphate, at a temperature, for example, in the range from 80 to 150° C.




Preparation of Intermediates
As a representative example, intermediate compound of Formula (504) wherein R4 is H, R2 is isopropyl, R3 is H and Pg is tert-butoxycarbonyl, can be prepared by following the reaction steps shown in Scheme 5. An intermediate compound of Formula (504) with variables R4, R2 and R3 can be prepared readily according to Scheme 5, or modifications thereof, using readily available starting materials and reagents.




To a solution of (2-bromo-5-chlorophenyl)methanol (60 g, 0.27 mol) in dry THF (600 mL) was added NaH (14.1 g, 0.35 mol, 60% in mineral oil) in portions at 0° C. under N2. The mixture was stirred at 0° C. for 1 h, and then MeI (84.9 g, 0.6 mol) was added dropwise to the reaction mixture at 0° C. The mixture was allowed to warm to rt and stirred for 1 h. The mixture was quenched with a sat. NH4Cl solution (600 mL) at 0° C. and extracted with DCM (3×500 mL). The combined organic layers were dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The crude residue was purified by column chromatography on silica gel (eluting with 5% ethyl acetate in petroleum ether) to afford 1-bromo-4-chloro-2-(methoxymethyl)benzene (58 g, 91%) as a colorless oil. 1H NMR (CDCl3 400 MHz): δ 7.54-7.43 (m, 2H), 7.13 (dd, J=2.8 Hz, 8.8 Hz, 1H), 4.48 (s, 2H), 3.49 (s, 3H). To a solution of isobutyraldehyde (77.4 g, 1.1 mol) and racemic 2-methylpropane-2-sulfinamide (86.7 g, 0.72 mol) in DCM (1 L) was added CuSO4 (172 g, 1.1 mol) at rt. The mixture was stirred at rt for 3 days. The mixture was filtered and the filtrate was concentrated under reduced pressure. The residue was purified by column chromatography on silica gel (eluting with 0.50% ethyl acetate in petroleum ether) to afford (E)-2-methyl-N-(2-methylpropylidene)propane-2-sulfinamide (82.0 g, 43%) as a yellow oil.
To a solution of 1-bromo-4-chloro-2-(methoxymethyl)benzene (18 g, 0.08 mol) in anhydrous THF (400 mL) was added n-BuLi (64 mL, 0.16 mol, 2.5 M in THF) dropwise at −78° C. under N2. After addition, the reaction mixture was stirred at −78° C. for 0.5 h, then a solution of (E)-2-methyl-N-(2-methylpropylidene)propane-2-sulfinamide (16 g, 0.09 mol) in THF (100 mL) was added dropwise to the reaction mixture at −78° C. under N2. The reaction solution was stirred at −78° C. for 0.5 h, and the mixture was allowed to warm to rt and stirred for 1 h. The reaction mixture was quenched with a sat. NH4Cl solution (500 mL) at 0° C. and extracted with DCM (3×300 mL). The combined organic layers were washed with brine (500 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica gel (eluting with a gradient of 10% to 50% ethyl acetate in petroleum ether) to afford racemic diastereomers of N-(1-(4-chloro-2-(methoxymethyl)phenyl)-2-methylpropyl)-2-methylpropane-2-sulfinamide as white solids. The more polar diastereomer was isolated as the major product (13.0 g, 51.4%) and the less polar diastereomer was isolated as the minor product (6.4 g, 25.3%). The more polar major diastereomer was used for the subsequent reactions for the isolations of the final compounds.
To a solution of N-(1-(4-chloro-2-(methoxymethyl)phenyl)-2-methylpropyl)-2-methylpropane-2-sulfinamide/more polar diastereomer from above (3 g, 9.06 mmol) in anhydrous DCM (20 mL) was added BBr3 (13 mL, 12.9 mmol, 1.0 M solution in DCM) dropwise at 0° C. under N2. After addition, the reaction mixture was allowed to warm to rt and stirred for 0.5 h.
The reaction mixture was cooled to 0° C., and MeOH (10 mL) was added carefully at 0° C. After addition, the reaction solution was allowed to warm to rt and stirred for 2 h. An additional 30 mL of MeOH was added and the reaction mixture was concentrated under reduced pressure. The residue was placed under high vacuum for 1 h resulting in the formation of a foamy tan solid. To this solid was added MeOH (30 mL), followed by Et3N (4.58 g, 45.3 mmol) at rt. After 5 min of stirring, Boc2O (4.0 g, 18.3 mmol) was added at rt. The reaction mixture was stirred for 1 h at rt. The mixture was concentrated under reduced pressure to remove the MeOH. DCM (30 mL) and water (30 mL) were added to the reaction mixture. The organic layer was extracted with DCM (3×30 mL). The combined organic layers were washed with brine (50 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude residue was purified by column chromatography on silica gel (eluting with a gradient of 1% to 2% ethyl acetate in petroleum ether) to afford tert-butyl-5-chloro-1-isopropylisoindoline-2-carboxylate (1.64 g, 61%) as a pale yellow solid. 1H NMR (CDCl3 400 MHz): δ 7.08-7.28 (m, 3H), 4.99 (s, 0.51H), 4.89 (s, 0.56H), 4.77 (d, J=15.2 Hz, 0.62H), 4.66 (d, J=15.2 Hz, 0.61H), 4.49 (s, 0.64H), 4.45 (s, 0.48H), 2.53-2.39 (m, 0.66H), 2.35-2.19 (m, 0.65H), 1.49 (s, 9H), 0.99 (d, J=7.2 Hz, 1.47H), 0.93 (d, J=7.2 Hz, 1.60H), 0.69 (d, J=6.8 Hz, 1.64H), 0.63 (d, J=6.8 Hz, 1.5H).
K2CO3 (4.3 g, 0.03 mol) and powdered 4 A molecular sieves (4.60 g) were added to a 250 mL round bottom flask. The flask was placed under high vacuum and the contents were dried with a heat gun for about 5 min. Upon cooling to rt, Pd(OAc)2 (186 mg, 0.8 mmol) and 1,3-bis(dicyclohexylphosphino)propane bis(tetrafluoroborate) (979 mg, 1.6 mmol) were added and the flask was placed under an N2 atmosphere. A solution of tert-butyl-5-chloro-1-isopropylisoindoline-2-carboxylate (6.1 g, 0.02 mol) in dry DMF (70 mL) was added to the mixture, followed by addition of n-butanol (24 ml, 0.2 mol). The contents were stirred while the flask was evacuated using a vacuum line and backfilled with about 1 atm of CO (2 balloons nested inside one another, filled with CO and attached to a syringe with a needle). This evacuation and backfilling process was repeated 4 times. The flask was then kept under bubbling with CO gas (from a gas bag with a volume of 30 L) and heated to 130° C. overnight, at which point LCMS analysis showed complete consumption of starting material with  ̃51% of butyl ester formation along with  ̃41% of the corresponding carboxylic acid product. The reaction was then cooled to rt and 200 mL of 1 N aqueous NaOH solution was added. After stirring for 1 h, the mixture was washed with ethyl acetate (3×100 mL). The aqueous layer was acidified with 2 N aqueous HCl solution to pH=3. The carboxylic acid product was then extracted out using EtOAc (4×100 mL) and the combined organic layers were washed with brine (3×100 mL) before drying and evaporating. The residue was purified by column chromatography on silica gel (eluting with a gradient of 20% to 50% ethyl acetate in petroleum ether) to afford 2-(tert-butoxycarbonyl)-1-isopropylisoindoline-5-carboxylic acid (3.80 g, 62%) as a white solid. LC-MS tR=5.093 min in 10-80AB_7 min chromatography (Welch Xtimate MK RP-18e 25-2 mm), MS (ESI) m/z 306.0 [M+H]+. 1H NMR (CDCl3 400 MHz): δ 8.05-7.95 (m, 2H), 7.36 (d, J=8.0 Hz, 0.48H), 7.32 (d, J=8.0 Hz, 0.56H), 5.11 (s, 0.49H), 5.01 (s, 0.53H), 4.88 (d, J=15.2 Hz, 0.54H), 4.77 (d, J=15.6 Hz, 0.51H), 4.59 (s, 0.61H), 4.55 (s, 0.47H), 2.53-2.45 (m, 0.62H), 2.40-2.30 (m, 0.69H), 1.52 (s, 9H), 1.04 (d, J=7.2 Hz, 1.5H), 0.98 (d, J=6.8 Hz, 1.7H), 0.73 (d, J=6.8 Hz, 1.6H), 0.68 (d, J=6.8 Hz, 1.5H).
As another representative example, intermediate compound of Formula (504) wherein R4 is H, R2 is ethyl, R3 is H and Pg is tert-butoxycarbonyl, can be prepared by following the reaction steps set forth in Scheme 5, except that (R,E)-2-methyl-N-propylidenepropane-2-sulfinamide was used (Scheme 6).




To a solution of (2-bromo-5-chlorophenyl)methanol (30.0 g, 135.4 mmol) in anhydrous THF (400 mL) was added NaH (8.1 g, 203.2 mmol, 60% in mineral oil) at 0° C. The reaction was stirred at 0° C. for 1 h. MeI (28.8 g, 203.2 mmol) was added dropwise via syringe over a 10 min period. After addition, the reaction mixture was stirred at rt for 2 h, at which point TLC analysis (petroleum ether:ethyl acetate=5:1) showed the complete consumption of starting material. The reaction was cooled down with an ice-water bath, quenched with water (100 mL) and diluted with ethyl acetate (1 L). The organic layer was washed with brine (3×500 mL), dried over anhydrous Na2SO4, filtered and concentrated in vacuo to afford the crude residue, which was purified by column chromatography on silica gel (eluting with petroleum ether) to give 1-bromo-4-chloro-2-(methoxymethyl)benzene (30 g, 94%) as a light brown oil. 1H NMR (CDCl3 400 MHz): δ 7.43-7.47 (m, 2H), 7.11-7.13 (m, 1H), 4.47 (s, 2H), 3.48 (s, 3H).
To a solution of propionaldehyde (10 g, 172.2 mmol) and (R)-2-methylpropane-2-sulfinamide (23 g, 189.4 mmol) in dry CH2Cl2 (500 mL) was added CuSO4 (41 g, 258.3 mmol) under a nitrogen atmosphere. The reaction was stirred for 20 h under a nitrogen atmosphere. The reaction was then filtered, washed with CH2Cl2 (3×300 mL) and concentrated in vacuo. The crude residue was purified by column chromatography on silica gel (eluting with petroleum ether:ethyl acetate=gradient of 50:1 to 15:1) to give (R,E)-2-methyl-N-propylidenepropane-2-sulfinamide (15 g, 54%) as a colorless oil. 1H NMR (CDCl3 400 MHz): δ 8.08 (t, J=4.4 Hz, 1H), 2.49-2.56 (m, 2H), 1.14-1.18 (m, 12H).
To a solution of 1-bromo-4-chloro-2-(methoxymethyl)benzene (6.0 g, 25.5 mmol) in anhydrous THF (50 mL) was added n-BuLi (20 mL, 51.0 mmol, 2.5 M in hexane) dropwise at −78° C. After addition, the reaction mixture was stirred at −78° C. for 30 min before adding a solution of (R,E)-2-methyl-N-propylidenepropane-2-sulfinamide (5.3 g, 33.1 mmol) in 15 mL of anhydrous THF dropwise. The reaction was stirred for 30 min at −78° C. and was slowly allowed to warm to rt. Stirring was continued at rt for 1 h, at which point TLC analysis (petroleum ether:ethyl acetate=20:1) showed complete consumption of the starting material. The reaction was cooled down to −78° C., quenched with a sat. NH4Cl solution (30 mL) then diluted with ethyl acetate (100 mL) and water (100 mL). The aqueous phase was extracted with ethyl acetate (3×80 mL). The organic layers were combined, washed with brine (3×200 mL), dried over anhydrous Na2SO4, filtered and evaporated in vacuo. The crude residue was purified by column chromatography on silica gel (eluting with petroleum ether:ethyl acetate=gradient of 20:1 to 1:1) to give the (R)—N—((R)-1-(4-chloro-2-(methoxymethyl)phenyl)propyl)-2-methylpropane-2-sulfinamide (2.0 g, 25%) and (R)—N—((S)-1-(4-chloro-2 (methoxymethyl)phenyl)propyl)-2-methylpropane-2-sulfinamide (1.2 g, 15%) as a solid. (R)—N—((R)-1-(4-chloro-2-(methoxymethyl)phenyl)propyl)-2-methylpropane-2-sulfinamide LC-MS: tR=1.054 min in 10-80AB_2.0 min chromatography (Welch Xtimate C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 318.1 [M+H]+. (R)—N—((S)-1-(4-chloro-2-(methoxymethyl)phenyl)propyl)-2-methylpropane-2-sulfinamide LC-MS: tR=0.793 min in 5-95AB_1.5 min chromatography (Welch Xtimate C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 318.0 [M+H]+.
To a solution of (R)—N—((S)-1-(4-chloro-2-(methoxymethyl)phenyl)propyl)-2-methylpropane-2-sulfinamide (1.2 g, 3.77 mmol) in anhydrous CH2Cl2 (10 mL) was added 5.6 mL of a 1M solution of BBr3 in CH2Cl2 at 0° C. under a nitrogen atmosphere. The reaction was allowed to warm to rt and stirred for 30 min. LCMS showed no starting material left and 4 peaks were observed corresponding to (S)-1-(2-(bromomethyl)-4-chlorophenyl)propan-1-amine, N—((S)-1-(4-chloro-2-(hydroxymethyl)phenyl)propyl)-2-methylpropane-2-sulfinamide, N—((S)-1-(2-(bromomethyl)-4-chlorophenyl)propyl)-2-methylpropane-2-sulfinamide and (S)-(2-(1-aminopropyl)-5-chlorophenyl)methanol. At this point, the reaction was cooled with an ice-water bath and 10 mL of MeOH was carefully added. The reaction was warmed to rt and stirred for 2 h before removing all of the solvents. 10 mL of MeOH was added to the residue and evaporated off again under reduced pressure. The resulting crude (S)-1-(2-(bromomethyl)-4-chlorophenyl)propan-1-amine was placed under high vacuum for 30 min resulting in the formation of a foamy tan solid. To this solid was added 8 mL of MeOH followed by Et3N (2.6 mL, 18.85 mmol, d=0.726 g/mL) at rt. After 5 min of stirring at rt, Boc2O (1.6 g, 7.54 mmol) was added. After 1 h, the MeOH was removed and the workup was done using ethyl acetate with water. The residue was purified by column chromatography on silica gel (eluting with petroleum ether:ethyl acetate=10:1) to give the (S)-tert-butyl 5-chloro-1-ethylisoindoline-2-carboxylate (850 mg, 80%) as a colorless oil. LC-MS tR=1.215 min in 10-80AB_2.0 min chromatography (Welch Xtimate C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 226.1, 228.1 [M−55]+.
K2CO3 (624 mg, 4.51 mmol) and powdered 4 Å molecular sieves (208 mg) were added to a 100 mL round bottom flask. The flask was placed under high vacuum and the contents were flamed dried for 5 min. Upon cooling to rt, Pd(OAc)2 (54 mg, 0.24 mmol) and 1,3-bis(dicyclohexylphosphino)propane bis(tetrafluoroborate) (294 mg, 0.48 mmol) were added to the flask. A solution of (S)-tert-butyl 5-chloro-1-ethylisoindoline-2-carboxylate (850 mg, 3.01 mmol) in anhydrous DMF (10 mL) was added followed by the addition of n-butanol (2.7 mL, 30.10 mmol, d=0.81 g/mL). The contents were stirred while the flask was evacuated using a vacuum line and backfilled with 1 atm of CO (2 balloons nested inside one another). This evacuation and backfilling process was repeated 4 times. The flask was kept under 1 atm of CO and heated to 100° C. overnight, at which point TLC analysis (petroleum ether:ethyl acetate=10:1) showed the starting material was consumed.
To the cooled reaction mixture was added 15 mL of a 1N NaOH solution at rt. After stirring for 30 min, the aqueous phase was washed with ethyl acetate (3×15 mL), acidified to pH=2 ̃3 with 1N HCl solution and extracted with ethyl acetate (3×30 mL). The combined organic layers were washed with brine (3×60 mL), dried over anhydrous Na2SO4, filtered and evaporated. The crude residue was purified by TFA preparative HPLC method A to give (S)-2-(tert-butoxycarbonyl)-1-ethylisoindoline-5-carboxylic acid (330 mg, 38%) as a brown solid and (S)-2-tert-butyl 5-butyl 1-ethylisoindoline-2,5-dicarboxylate (200 mg, 19%) as a yellow oil. LC-MS tR=1.288 min in 10-80AB_2.0 min chromatography (Welch Xtimate C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 292.1 [M−55]+.
To a solution of (S)-2-tert-butyl 5-butyl 1-ethylisoindoline-2,5-dicarboxylate (200 mg, 0.57 mmol) in MeOH (2 mL) and THF (2 mL) was added NaOH solution (1 mL, 10% w/w in water). After addition, the reaction mixture was stirred at 20 ̃24° C. for 16 h. The reaction mixture was then acidified to pH=2 ̃4 with 1 N HCl solution, diluted with water (10 mL) and extracted with ethyl acetate (3×15 mL). The combined organics were washed with brine (3×30 mL), dried over anhydrous Na2SO4, filtered and evaporated to afford (S)-2-(tert-butoxycarbonyl)-1-ethylisoindoline-5-carboxylic acid (160 mg, 96%) as a brown solid. LC-MS tR=1.021 min in 10-80AB_2.0 min chromatography (Welch Xtimate C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 236.1 [M−55]+.
(R)-2-(tert-butoxycarbonyl)-1-ethylisoindoline-5-carboxylic acid




Procedure same as that for (S)-2-(tert-butoxycarbonyl)-1-ethylisoindoline-5-carboxylic acid, outlined in Scheme 6, using (R)—N—((R)-1-(4-chloro-2-(methoxymethyl)phenyl)propyl)-2-methylpropane-2-sulfinamide as the starting material. LC-MS tR=1.017 min in 10-80AB_2.0 min chromatography (Welch Xtimate C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 236.1 [M−55]+.
(5-(ethylsulfonyl)pyridin-2-yl)methanamine was prepared following the synthetic route shown in Scheme 7.




To a flame dried flask equipped with a stir bar was added tert-butyl ((5-bromopyridin-2-yl)methyl)carbamate (2.92 g, 10.2 mmol), ethane sulfinic acid sodium salt (2.36 g, 20.3 mmol), L-proline (234 mg, 2.03 mmol), copper (I) iodide (194 mg, 1.02 mmol) and sodium hydroxide (81.3 mg, 2.03 mmol). The flask was purged with N2, then DMSO (35 mL) was added. The reaction mixture was heated to 110° C. and stirred for 15 h. The flask was then cooled to rt and the mixture was partitioned between EtOAc (150 mL) and saturated aqueous ammonium chloride (150 mL). The organic phase was separated, washed with brine (50 mL), dried over anhydrous MgSO4, filtered and concentrated under reduced pressure. The residue was purified by silica gel chromatography (eluting with 35% EtOAc in hexanes, gradient to 60%) to afford tert-butyl((5-bromopyridin-2-yl)methyl)carbamate (1.81 g, 59%). LC-MS tR=0.74 min in 1 min chromatography, MS (ESI) m/z 301.4 [M+H]+. 1H NMR (CDCl3, 400 MHz): δ 9.02 (dd, J=0.8 Hz, 2.0 Hz, 1H), 8.15 (dd, J=2.4 Hz, 8.4 Hz, 1H), 7.49 (dd, J=0.8 Hz, 8.4 Hz, 1H), 5.49 (broad s, 1H), 4.55 (d, J=7.0 Hz, 2H), 3.15 (q, J=7.2 Hz, 2H), 1.47 (s, 9H), 1.31 (t, J=7.2 Hz, 3H).
To a solution of tert-butyl ((5-bromopyridin-2-yl)methyl)carbamate (1.81 g, 6.03 mmol) in MeOH (40 mL) at 0° C. was added acetyl chloride (4.30 mL, 60.3 mmol) dropwise over 5 min. The solution was allowed to warm to rt and was stirred for 3 h. The mixture was concentrated under reduced pressure to yield 1.64 g (5-(ethylsulfonyl)pyridin-2-yl)methanamine bis-hydrochloride salt ( ̃100%). This material was used directly for the next step without purification. LC-MS tR=0.25 min in 1 min chromatography, MS (ESI) m/z 201.2 [M+H]+. 1H NMR (CD3OD, 400 MHz): δ 9.09 (d, J=1.2 Hz, 1H), 8.35 (dd, J=2.4 Hz, 8.4 Hz, 1H), 7.49 (d, J=8.4 Hz, 1H), 4.45 (s, 2H), 3.31 (q, J=7.2 Hz, 2H), 1.26 (t, J=7.2 Hz, 3H).
N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropylisoindoline-5-carboxamide was prepared following the synthetic route shown in Scheme 8.




To a solution of (5-(ethylsulfonyl)pyridin-2-yl)methanamine (HCl salt, 290 mg, 1.23 mmol) in anhydrous DCM (2 mL) was added DIPEA (318 mg, 2.46 mmol), HATU (622 mg, 1.64 mmol) and 2-(tert-butoxycarbonyl)-1-isopropylisoindoline-5-carboxylic acid (250 mg, 0.82 mmol). The mixture was stirred at 25° C. for 2 h. The mixture was filtered and the filtrate was washed with H2O (5 mL) and extracted with DCM (3×5 mL). The combined organic layers were washed with H2O (5 mL) then brine (5 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica gel (eluting with 5% MeOH in DCM) to afford tert-butyl 5-(((5-(ethylsulfonyl)pyridin-2-yl)methyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate (125 mg, 50%) as a white solid. A portion of this material was purified by SFC separation to afford pure enantiomers of both tert-butyl (S)-5-(((5-(ethylsulfonyl)pyridin-2-yl)methyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate and tert-butyl (R)-5-(((5-(ethylsulfonyl)pyridin-2-yl)methyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate.
To a solution of tert-butyl 5-((5-(ethylsulfonyl)pyridin-2-yl)methyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate (50 mg, 0.102 mmol) in CH2Cl2 (2 mL) was added TFA (1 mL). The mixture was stirred at rt for 1 h. The reaction was neutralized with aqueous NaHCO3 to pH=9-10. The mixture was extracted with CH2Cl2 (3×5 mL). The combined organic layers were washed with water (5 mL) then brine (5 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to afford crude N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropylisoindoline-5-carboxamide (50 mg, 100%) as a white solid, which was used for the next step directly without further purification. For some Examples, enantiomercially pure tert-butyl (S)-5-(((5-(ethylsulfonyl)pyridin-2-yl)methyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate and tert-butyl (R)-5-(((5-(ethylsulfonyl)pyridin-2-yl)methyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate were subjected to the same conditions to afford enantiomerically pure (S)—N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropylisoindoline-5-carboxamide and (R)—N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropylisoindoline-5-carboxamide, respectively.
(S)-1-ethyl-N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)isoindoline-5-carboxamide was prepared following the synthetic route shown in Scheme 9.




To a solution of (S)-2-(tert-butoxycarbonyl)-1-ethylisoindoline-5-carboxylic acid (40 mg, 0.14 mmol) and (5-(ethylsulfonyl)pyridin-2-yl)methanamine HCl salt (42 mg, 0.21 mmol) in DMF (1 mL) was added HATU (106 mg, 0.28 mmol) and DIEA (122 uL, 0.70 mmol, d=0.782 g/mL) under N2 at rt. After addition, the reaction was stirred at rt for 16 h. The reaction mixture was then diluted with ethyl acetate (30 mL), washed with brine (20 mL), dried over anhydrous Na2SO4, filtered and evaporated. The crude residue was purified by preparative TLC (eluting with petroleum ether:ethyl acetate=1:4) to give (S)-tert-butyl 1-ethyl-5-(((5-(ethylsulfonyl)pyridin-2-yl)methyl)carbamoyl)isoindoline-2-carboxylate (30 mg, 45%) as a brown solid. LCMS tR=1.110 min in 10-80AB_2.0 min chromatography (Xbridge Shield RP18 2.1*50 mm), MS (ESI) m/z 474.1 [M+H]+.
To a solution of (S)-tert-butyl 1-ethyl-5-(((5-(ethylsulfonyl)pyridin-2-yl)methyl)carbamoyl)isoindoline-2-carboxylate (50 mg, 0.10 mmol) in dichloromethane (2 mL) was added HCl/dioxane (0.5 mL, 2.0 mmol, 4N) under a nitrogen atmosphere. After addition, the reaction mixture was stirred at rt for 16 h. The reaction mixture was then basified to pH=11 ̃13 with 1 N NaOH solution. The mixture was diluted with water (10 mL) and extracted with ethyl acetate (3×15 mL). The combined organics were washed with brine (3×30 mL), dried over anhydrous Na2SO4, filtered and evaporated to afford crude (S)-1-ethyl-N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)isoindoline-5-carboxamide (20 mg, 85%) as a brown solid. LC-MS tR=1.249 min in 10-80CD_POS_3.0 min chromatography (Welch Xtimate C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 374.1 [M+H]+.
(R)-1-ethyl-N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)isoindoline-5-carboxamide




Procedure same as that for (S)-1-ethyl-N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)isoindoline-5-carboxamide using (R)-2-(tert-butoxycarbonyl)-1-ethylisoindoline-5-carboxylic acid as the starting material. LC-MS tR=1.253 min in 10-80CD_POS_3.0 min chromatography (Welch Xtimate C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 374.1 [M+H]+.
(4-(ethylsulfonyl)phenyl)methanamine was prepared following the synthetic route shown in Scheme 10.




To a mixture of 4-methylbenzenethiol (100 g, 0.8 mol) in acetone (1 L) was added iodoethane (190 g, 1.2 mol) and potassium carbonate (220 g, 1.6 mol). The mixture was stirred at 60° C. overnight. The mixture was filtered and the filtrate was concentrated under reduced pressure to afford crude ethyl(p-tolyl)sulfane (120 g, 99%) as a yellow solid, which was used for the next step without further purification.
To a solution of crude ethyl(p-tolyl)sulfane (35 g, 0.23 mol) in CH2Cl2 (1.5 L) was added m-chloroperoxybenzoic acid (101 g, 0.59 mol) at 0° C. The mixture was stirred at rt overnight. The mixture was filtered. The filtrate was added to saturated aqueous Na2SO3 (500 mL) slowly and then stirred for 0.5 h. After partitioning, the organic layer was washed with saturated aqueous NaHCO3 (500 mL), dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to afford crude 1-(ethylsulfonyl)-4-methylbenzene (42.3 g, 100%) as a pale yellow solid. This material was used for the next step without further purification.
To a solution of 1-(ethylsulfonyl)-4-methylbenzene (5 g, 25.7 mmol) in CCl4 (30 mL) was added N-bromosuccinimide (5.54 g, 30.8 mmol) and azobisisobutyronitrile (0.46 g, 2.57 mmol). The mixture was stirred at 80° C. overnight. The mixture was filtered and the filtrate was concentrated under reduced pressure. The residue was added to water (50 mL) and extracted with EtOAc (3×30 mL). The combined organic layers were washed with water (2×40 mL) then brine (50 mL), dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to afford crude 1-(bromomethyl)-4-(ethylsulfonyl)benzene (6.62 g, 98%) as a yellow solid. This material was used for the next step without further purification.
To a solution of 1-(bromomethyl)-4-(ethylsulfonyl)benzene (6.62 g, 25.2 mmol) in MeOH (30 mL) was added a 28% aqueous ammonium hydroxide solution (30 mL). The mixture was stirred at rt overnight. The mixture was then concentrated under reduced pressure. The residue was purified by basic preparative HPLC method B separation to afford (4-(ethylsulfonyl)phenyl)methanamine (1.5 g, 30%) as a yellow solid. LC-MS tR=1.747 min in 0-30CD_3 min chromatography (Durashell C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 200.0 [M+H]+ and 399.0 [2M+H]+. 1H NMR (CDCl3, 400 MHz): δ 7.85 (d, J=8.0 Hz, 2H), 7.53 (d, J=8.0 Hz, 2H), 3.98 (s, 2H), 3.10 (q, J=7.6 Hz, 2H), 1.26 (t, J=7.6 Hz, 3H).
Tert-butyl 5-((4-(ethylsulfonyl)benzyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate




Procedure same as that for tert-butyl-5-(((5-(ethylsulfonyl)pyridin-2-yl)methyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate using (4-(ethylsulfonyl)phenyl)methanamine as the starting material.
N-(4-(ethylsulfonyl)benzyl)-1-isopropylisoindoline-5-carboxamide




Procedure same as that for N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropylisoindoline-5-carboxamide using tert-butyl 5-((4-(ethylsulfonyl)benzyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate as the starting material. The crude product was used directly for the next step without further purification. LC-MS tR=0.522 min in 5-95 AB_1.5 min chromatography (MK RP-18e 25-2 mm), MS (ESI) m/z 387.0 [M+H]+.
(R)-2-amino-2-(4-(ethylsulfonyl)phenyl)ethan-1-ol (3:2 mixture of enantiomers, R:S) was prepared following the synthetic route shown in Scheme 11.




To a suspension of NaH (12.9 g, 0.322 mol, 60% in mineral oil) in dry THF (500 mL) was added a solution of ethane-1,2-diol (20.0 g, 0.322 mol) in dry THF (100 mL) dropwise. The mixture was stirred for 1 h at rt, then TBSCl (48.59 g, 0.322 mol) was added and the mixture was stirred for another 1 h at rt. The mixture was quenched with an aqueous K2CO3 solution (100 mL, 10%) and extracted with MTBE (3×300 mL). The combined organic layers were dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The crude residue was purified by column chromatography on silica gel (eluting with a gradient of 1% to 33% ethyl acetate in petroleum ether) to afford 2-((tert-butyldimethylsilyl)oxy)ethanol (55.0 g, 96%) as a colorless oil.
To a solution of (COCl)2 (13.7 mL, 162.2 mmol) in anhydrous CH2Cl2 (400 mL) was added DMSO (25.1 mL, 353.9 mmol) at −78° C. under N2. After being stirred for 30 min, a solution of 2-((tert-butyldimethylsilyl)oxy)ethanol (26.0 g, 147.5 mmol) in anhydrous CH2Cl2 (100 mL) was added dropwise. The mixture was stirred at −78° C. for 30 min and then Et3N (102.74 mL, 737.0 mmol) was added dropwise. After being stirred at −78° C. for 30 min, the reaction was allowed to warm to rt and stirred for 1 h. The reaction mixture was acidified with 2 N aqueous HCl solution to pH=4 and then extracted with CH2Cl2 (3×400 mL). The combined organic layers were dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to afford 2-((tert-butyldimethylsilyl)oxy)acetaldehyde (34.0 g, 100%) as a colorless oil. This material was used for the next step directly without further purification.
To a solution of 2-((tert-butyldimethylsilyl)oxy)acetaldehyde (34.0 g, 147.5 mmol) and (R)-2-methylpropane-2-sulfinamide (19.6 g, 162.2 mmol) in DCM (400 mL) was added CuSO4 (47.2 g, 295.0 mmol) at rt. The mixture was stirred at 25° C. for 48 h. The mixture was filtered and the filtrate was concentrated under reduced pressure. The residue was purified by column chromatography on silica gel (eluting with a gradient of 1% to 10% ethyl acetate in petroleum ether) to afford (R,E)-N-(2-((tert-butyldimethylsilyl)oxy)ethylidene)-2-methylpropane-2-sulfinamide (30.0 g, 73%) as a yellow oil. 1H NMR (CDCl3 400 MHz): δ 7.96 (t, J=3.2 Hz, 1H), 4.45 (d, J=3.2 Hz, 2H), 1.11 (s, 9H), 0.82 (s, 9H), 0.00 (s, 6H).
To a solution of (4-bromophenyl)(ethyl)sulfane (1.88 g, 8.65 mmol) in anhydrous THF (15 mL) was added n-BuLi (14.4 mL, 36.0 mmol, 2.5 M in THF) at −78° C. under N2. The reaction mixture was stirred at −78° C. for 0.5 h, then a solution of (R,E)-N-(2-((tert-butyldimethylsilyl)oxy)ethylidene)-2-methylpropane-2-sulfinamide (2.0 g, 7.2 mmol) in THF (1 mL) was added dropwise to the mixture at −78° C. The reaction mixture was stirred at −78° C. for 0.5 h and was allowed to warm to rt. After 0.5 h at rt, the reaction mixture was quenched with water (10 mL) and extracted with ethyl acetate (3×20 mL). The combined organic layers were washed with H2O (10 mL) then brine (10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure.
The crude residue was purified by column chromatography on silica gel (eluting with a gradient of 5% to 10% ethyl acetate in petroleum ether) to afford a 3:2 mixture of (R)—N—((R)-2-((tert-butyldimethylsilyl)oxy)-1-(4-(ethylthio)phenyl)ethyl)-2-methylpropane-2-sulfinamide and (R)—N—((S)-2-((tert-butyldimethylsilyl)oxy)-1-(4-(ethylthio)phenyl)ethyl)-2-methylpropane-2-sulfinamide (2.86 g, 47.9%) as a yellow oil. This 3:2 mixture of diastereomers was used for the subsequent reactions. 1H NMR (CDCl3 400 MHz): δ 7.24-7.16 (m, 4H), 4.47-4.40 (m, 1H), 4.21 (s, 1H), 3.75-3.67 (m, 1H), 3.60-3.47 (m, 1H), 2.90 (q, J=7.2 Hz, 2H), 1.27 (t, J=7.2 Hz, 3H), 1.18 (s, 9H), 0.85 (s, 9H), 0.02 (s, 3H), 0.00 (s, 3H).
To a solution of (R)—N—((R)-2-((tert-butyldimethylsilyl)oxy)-1-(4-(ethylthio)phenyl)ethyl)-2-methylpropane-2-sulfinamide (2.86 g, 6.86 mmol), as a 3:2 mixture of diastereomers from previous step, in dioxane (10 mL) was added HCl in dioxane (20 mL, 4 M) at rt. The mixture was stirred for 3 h at rt and was then concentrated under reduced pressure to afford crude (R)-2-amino-2-(4-(ethylthio)phenyl)ethan-1-ol HCl salt (3.0 g, 100%) as a white solid. This crude material was a 3:2 mixture of enantiomers (R:S) and used for the next step without further purification.
To a solution of (R)-2-amino-2-(4-(ethylthio)phenyl)ethan-1-ol HCl salt (3.0 g, 6.86 mmol), as a 3:2 mixture of enantiomers/R:S, in H2O (30 mL) was added Oxone® monopersulfate (8.4 g, 13.72 mmol) at rt. The mixture was stirred for 1.5 h at rt and was lyophilized directly. After lyophilization, the crude product was purified by flash column chromatography on silica gel (eluting with MeOH) to give crude (R)-2-amino-2-(4-(ethylsulfonyl)phenyl)ethan-1-ol HCl salt (3:2 mixture of enantiomers, R:S) (1.25 g, 80%) as a yellow solid. 1H NMR (CDCl3 400 MHz): δ 7.99 (d, J=8.4 Hz, 2H), 7.73 (d, J=8.0 Hz, 2H), 4.55-4.50 (m, 1H), 3.99-3.91 (m, 1H), 3.85-3.80 (m, 1H), 3.23 (q, J=7.2 Hz, 2H), 1.21 (t, J=7.2 Hz, 3H).
(S)-1-ethyl-N—((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)isoindoline-5-carboxamide (3:2 mixture of diastereomers) was prepared following the synthetic route shown in Scheme 12.




To a solution of (S)-2-(tert-butoxycarbonyl)-1-ethylisoindoline-5-carboxylic acid (120 mg, 0.41 mmol) and (R)-2-amino-2-(4-(ethylsulfonyl)phenyl)ethanol (113 mg, 0.49 mmol), as a 3:2 mixture of enantiomers (R:S), in DMF (5 mL) was added HATU (312 mg, 0.82 mmol) and DIEA (0.36 mL, 2.05 mmol, d=0.782 g/mL) under a nitrogen atmosphere. After addition, the reaction mixture was stirred at 24 ̃27° C. for 18 h. TLC analysis (eluting with CH2Cl2:MeOH=10:1) showed complete consumption of the starting material. The reaction mixture was diluted with ethyl acetate (30 mL), washed with brine (3×20 mL), dried over anhydrous Na2SO4, filtered and evaporated. The crude residue was purified by preparative TLC (eluting with CH2Cl2:MeOH=10:1) to give a 3:2 mixture of (S)-tert-butyl 1-ethyl-5-(((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)carbamoyl)isoindoline-2-carboxylate and (S)-tert-butyl 1-ethyl-5-(((S)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)carbamoyl)isoindoline-2-carboxylate (180 mg, 41%, 47% chemical purity) as a brown oil. LC-MS tR=1.223 min in 10-80AB_2.0 min chromatography (Welch Xtimate C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 503.2 [M+H]+.
To a solution of (S)-tert-butyl 1-ethyl-5-(((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)carbamoyl)isoindoline-2-carboxylate and (S)-tert-butyl 1-ethyl-5-(((S)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)carbamoyl)isoindoline-2-carboxylate (180 mg, 0.17 mmol, 47% chemical purity) in dichloromethane (4.0 mL) was added HCl/dioxane (1.0 mL, 4.0 mmol, 4N) under a nitrogen atmosphere. After addition, the reaction mixture was stirred at 23 ̃30° C. for 16 h. TLC analysis (eluting with CH2Cl2:MeOH=10:1) showed complete consumption of the starting material. The solvents were removed under reduced pressure to afford the residue which was purified by basic preparative HPLC method C to give a 3:2 mixture of (S)-1-ethyl-N—((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)isoindoline-5-carboxamide and (S)-1-ethyl-N—((S)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)isoindoline-5-carboxamide (70 mg, 100%) as a white solid. LC-MS tR=1.276 min in 10-80CD_POS_3.0 min chromatography (Welch Xtimate C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 403.1 [M+H]+.
Tert-butyl (R)-1-ethyl-5-(((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)carbamoyl)isoindoline-2-carboxylate:




Procedure same as that for (S)-tert-butyl 1-ethyl-5-(((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)carbamoyl)isoindoline-2-carboxylate using (R)-2-(tert-butoxycarbonyl)-1-ethylisoindoline-5-carboxylic acid and (R)-2-amino-2-(4-(ethylsulfonyl)phenyl)ethanol (3:2 mixture of enantiomers/R:S) as the starting materials. LC-MS tR=1.095 min in 10-80AB_2.0 min chromatography (Welch Xtimate C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 503.1 [M+H]+.
(R)-1-ethyl-N— ((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)isoindoline-5-carboxamide




Procedure same as that for (S)-1-ethyl-N— ((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)isoindoline-5-carboxamide using tert-butyl (R)-1-ethyl-5-(((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)carbamoyl)isoindoline-2-carboxylate (3:2 mixture of diastereomers) as the starting material. LC-MS tR=1.252 min in 10-80CD_POS_3.0 min chromatography (Welch Xtimate C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 403.1 [M+H]+.
Tert-butyl 5-((1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate




Procedure same as that for (S)-tert-butyl 1-ethyl-5-(((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)carbamoyl)isoindoline-2-carboxylate using 2-(tert-butoxycarbonyl)-1-isopropylisoindoline-5-carboxylic acid and (R)-2-amino-2-(4-(ethylsulfonyl)phenyl)ethanol (3:2 mixture of enantiomers/R:S) as the starting materials. Most of this intermediate was used for the subsequent reactions as a mixture of 4 diastereomers which was ultimately subjected to SFC separation after the final step of the sequence. However, a portion of this intermediate was purified by SFC separation at this stage to afford pure diastereomers of tert-butyl (S)-5-(((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate and tert-butyl (R)-5-(((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate. In some Examples, these enantiomercially pure intermediates were used in subsequent reactions to isolate final compounds. LC-MS tR=0.777 min in 5-95 AB_1.5 min chromatography (Welch MERCK RP-18e 25-2 mm), MS (ESI) m/z 539.0 [M+Na]+.
N-(1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)-1-isopropylisoindoline-5-carboxamide




Procedure same as that for (S)-1-ethyl-N—((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)isoindoline-5-carboxamide using tert-butyl 5-((1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate as the starting material. The crude product was used directly for the next step without further purification. For some Examples, tert-butyl (S)-5-(((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate and tert-butyl (R)-5-(((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate were subjected to the same conditions to afford enantiomercially pure (S)—N—((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)-1-isopropylisoindoline-5-carboxamide and (R)—N—((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)-1-isopropylisoindoline-5-carboxamide, respectively. In some Examples, these enantiomercially pure intermediates were used in subsequent reactions to isolate final compounds.
(R)-2-amino-2-(5-(ethylsulfonyl)pyridin-2-yl)ethan-1-ol (3:2 mixture of enantiomers, R:S) was prepared following the synthetic route shown in Scheme 13.




To a mixture of 2-bromo-5-fluoropyridine (6.28 g, 35.66 mmol) in anhydrous DMF (60 mL) was added NaSEt (3 g, 35.66 mmol). The mixture was stirred at 100° C. for 3 h under N2. TLC analysis (eluting with 10% ethyl acetate in petroleum ether) showed that the starting material was not consumed completely, therefore additional NaSEt (0.9 g, 9.56 mmol) was added to the mixture. The mixture was stirred at 100° C. for another 12 h under N2. The mixture was added to H2O (150 mL) and extracted with ethyl acetate (3×150 mL). The combined organic layers were washed with brine (400 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica gel (eluting with 2% ethyl acetate in petroleum ether) to afford 2-bromo-5-(ethylthio)pyridine (7.0 g, 90%) as a colorless oil. LC-MS tR=0.717 min in 5-95AB_1.5 min chromatography (Welch Merck RP-18e 25-2 mm), MS (ESI) m/z 217.6 [M+H]+.
To a solution of toluene (60 mL) was added n-BuLi (10.6 mL, 26.48 mmol, 2.5 M in hexane) dropwise at −78° C. under N2 such that the internal temperature did not exceed −50° C. Then a solution of 2-bromo-5-(ethylthio)pyridine (3.85 g, 17.65 mmol) in toluene (10 mL) was added to the reaction mixture at −78° C. such that the internal temperature did not exceed −65° C. The mixture was stirred at −78° C. for 1 h under N2. A solution of (R,E)-N-(2-((tert-butyldimethylsilyl)oxy)ethylidene)-2-methylpropane-2-sulfinamide (4.90 g, 17.65 mmol) in toluene (10 mL) was added to the reaction mixture at −78° C. such that the internal temperature did not exceed −60° C. The mixture was stirred at −78° C. for another 2 h under N2. The mixture was quenched with brine (150 mL) at −78° C. and extracted with ethyl acetate (3×150 mL). The combined organic layers were washed with brine (400 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica gel (eluting with a gradient of 10% to 33% ethyl acetate in petroleum ether) to afford a 3:2 mixture of (R)—N—((R)-2-((tert-butyldimethylsilyl)oxy)-1-(5-(ethylthio)pyridin-2-yl)ethyl)-2-methylpropane-2-sulfinamide and (R)—N—((S)-2-((tert-butyldimethylsilyl)oxy)-1-(5-(ethylthio)pyridin-2-yl)ethyl)-2-methylpropane-2-sulfinamide (3.0 g, 41%) as a pale yellow oil. This 3:2 mixture of diastereomers was used for the subsequent reactions. LC-MS tR=1.014 min in 5-95AB_1.5 min chromatography (Welch Merck RP-18e 25-2 mm), MS (ESI) m/z 417.2 [M+H]+.
(R)—N—((R)-2-((tert-butyldimethylsilyl)oxy)-1-(5-(ethylthio)pyridin-2-yl)ethyl)-2-methylpropane-2-sulfinamide (4.9 g, 11.76 mmol), as a 3:2 mixture of diastereomers from the previous step, in 1,4-dioxane (30 mL) was added HCl/dioxane (12 mL, 4 N) at 0° C. The mixture was stirred at rt for 2 h. The mixture was concentrated under reduced pressure to afford crude (R)-2-amino-2-(5-(ethylthio)pyridin-2-yl)ethan-1-ol HCl salt (2.5 g, 91%) as a yellow solid. This material was a 3:2 mixture of enantiomers (R:S) and was used for the next step directly without further purification.
To a mixture of (R)-2-amino-2-(5-(ethylthio)pyridin-2-yl)ethan-1-ol HCl salt (2.5 g, 10.66 mmol) in H2O (40 mL) was added Oxone® monopersulfate (13.1 g, 21.32 mmol). The mixture was stirred at rt for 2 h. CH3CN (15 mL) was added and the reaction mixture was lyophilized. After lyophilization, the crude product was purified by flash column chromatography on silica gel (eluting with MeOH). After concentration, the residue was purified again by column chromatography on silica gel (eluting with 10% MeOH in DCM) to afford (R)-2-amino-2-(5-(ethylsulfonyl)pyridin-2-yl)ethan-1-ol (3:2 mixture of enantiomers/R:S) (1.9 g, 67%) as a black solid. 1H NMR (CD3OD 400 MHz): δ 9.08 (s, 1H), 8.35 (dd, J=2.0, 8.4 Hz, 1H), 7.79 (d, J=8.4 Hz, 1H), 4.70 (t, J=5.6 Hz, 1H), 4.03 (dd, J=4.8, 12.0 Hz, 1H), 3.91 (dd, J=4.8, 11.6 Hz, 1H), 3.29 (q, J=7.2 Hz, 2H), 1.25 (t, J=7.2 Hz, 3H).
Tert-butyl 5-(((R)-1-(5-(ethylsulfonyl)pyridin-2-yl)-2-hydroxyethyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate




Procedure same as that for (S)-tert-butyl 1-ethyl-5-(((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)carbamoyl)isoindoline-2-carboxylate using 2-(tert-butoxycarbonyl)-1-isopropylisoindoline-5-carboxylic acid and (R)-2-amino-2-(5-(ethylsulfonyl)pyridin-2-yl)ethan-1-ol (3:2 mixture of enantiomers, R:S) as the starting materials. LC-MS tR=0.778 min in 5-95AB_1.5 min chromatography (Welch MERCK RP-18e 25-2 mm), MS (ESI) m/z 518.0 [M+H]+.
N—((R)-1-(5-(ethylsulfonyl)pyridin-2-yl)-2-hydroxyethyl)-1-isopropylisoindoline-5-carboxamide




Procedure same as that for (S)-1-ethyl-N— ((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)isoindoline-5-carboxamide using tert-butyl 5-(((R)-1-(5-(ethylsulfonyl)pyridin-2-yl)-2-hydroxyethyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate as the starting material.
Tert-butyl (S)-1-ethyl-5-(((R)-1-(5-(ethylsulfonyl)pyridin-2-yl)-2-hydroxyethyl)carbamoyl)isoindoline-2-carboxylate




Procedure same as that for (S)-tert-butyl 1-ethyl-5-(((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)carbamoyl)isoindoline-2-carboxylate using (R)-2-amino-2-(5-(ethylsulfonyl)pyridin-2-yl)ethan-1-ol (3:2 mixture of enantiomers, R:S) as the starting material. LCMS tR=0.711 min in 5-95AB_1.5 min chromatography (MK RP18e 25-2 mm), MS (ESI) m/z 504.1 [M+H]+.
(S)-1-ethyl-N—((R)-1-(5-(ethylsulfonyl)pyridin-2-yl)-2-hydroxyethyl)isoindoline-5-carboxamide




Procedure same as that for (S)-1-ethyl-N— ((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)isoindoline-5-carboxamide using tert-butyl (S)-1-ethyl-5-(((R)-1-(5-(ethylsulfonyl)pyridin-2-yl)-2-hydroxyethyl)carbamoyl)isoindoline-2-carboxylate as the starting material.
Tert-butyl (R)-1-ethyl-5-(((R)-1-(5-(ethylsulfonyl)pyridin-2-yl)-2-hydroxyethyl)carbamoyl)isoindoline-2-carboxylate




Procedure same as that for (S)-tert-butyl 1-ethyl-5-(((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)carbamoyl)isoindoline-2-carboxylate using (R)-2-(tert-butoxycarbonyl)-1-ethylisoindoline-5-carboxylic acid and (R)-2-amino-2-(5-(ethylsulfonyl)pyridin-2-yl)ethan-1-ol (3:2 mixture of enantiomers, R:S) as the starting materials. LCMS tR=0.715 min in 5-95AB_1.5 min chromatography (MK RP18e 25-2 mm), MS (ESI) m/z 504.2 [M+H]+.
(R)-1-ethyl-N—((R)-1-(5-(ethylsulfonyl)pyridin-2-yl)-2-hydroxyethyl)isoindoline-5-carboxamide




Procedure same as that for (S)-1-ethyl-N—((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)isoindoline-5-carboxamide using tert-butyl (R)-1-ethyl-5-(((R)-1-(5-(ethylsulfonyl)pyridin-2-yl)-2-hydroxyethyl)carbamoyl)isoindoline-2-carboxylate as the starting material.
(1-(methylsulfonyl)piperidin-4-yl)methanamine was prepared following the synthetic route shown in Scheme 14.




To a mixture of tert-butyl (piperidin-4-ylmethyl)carbamate (500 mg, 2.34 mmol) and Et3N (1.18 g, 1.63 mL, 11.7 mmol) in CH2Cl2 (8 mL) was added MsCl (536 mg, 4.68 mmol) at 0° C. The mixture was stirred at rt overnight. TLC (CH2Cl2:MeOH=2:1) showed that the tert-butyl(piperidin-4-ylmethyl)carbamate was consumed. The mixture was added with CH2Cl2 (30 mL), washed with 1 N HCl (3×20 mL) and then brine (20 mL). The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give tert-butyl ((1-(methylsulfonyl)piperidin-4-yl)methyl)carbamate (650 mg, 95%) as a yellow solid, which was used for the next step directly without further purification. 1H NMR (CDCl3 400 MHz): δ 4.65 (brs, 1H), 3.81 (d, J=12.4 Hz, 2H), 3.05 (t, J=6.4 Hz, 2H), 2.77 (s, 3H), 2.65 (t, J=11.6 Hz, 2H), 1.81 (d, J=12.4 Hz, 2H), 1.62-1.57 (m, 1H), 1.44 (s, 9H), 1.37-1.30 (m, 2H).
To a mixture of tert-butyl-((1-(methylsulfonyl)piperidin-4-yl)methyl)carbamate (650 mg, 2.22 mmol) in dioxane (5 mL) was added HCl/dioxane (5 mL, 4N). The mixture was stirred at rt for 3 h. The mixture was concentrated under reduced pressure to give (1-(methylsulfonyl)piperidin-4-yl)methanamine HCl salt (500 mg, 98%) as a white solid, which was used for the next step directly without further purification. 1H NMR (DMSO-d6 400 MHz): δ 7.93 (brs, 3H), 3.55-3.53 (m, 2H), 2.85 (s, 3H), 2.74-2.65 (m, 4H), 1.80 (d, J=13.2 Hz, 2H), 1.70-1.60 (m, 1H), 1.25-1.17 (m, 2H).
Tert-butyl 1-isopropyl-5-(((1-(methylsulfonyl)piperidin-4-yl)methyl)carbamoyl)isoindoline-2-carboxylate




Procedure same as that for tert-butyl-5-(((5-(ethylsulfonyl)pyridin-2-yl)methyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate using (1-(methylsulfonyl)piperidin-4-yl)methanamine HCl salt as the starting material. Most of this intermediate was used for the subsequent reactions as a mixture of enantiomers which was ultimately subjected to SFC separation after the final step of the sequence. However, a portion of this intermediate was purified by SFC separation at this stage to afford pure enantiomers of tert-butyl (S)-1-isopropyl-5-(((1-(methylsulfonyl)piperidin-4-yl)methyl)carbamoyl)isoindoline-2-carboxylate and tert-butyl (R)-1-isopropyl-5-(((1-(methylsulfonyl)piperidin-4-yl)methyl)carbamoyl)isoindoline-2-carboxylate. LCMS tR=0.786 min in 5-95AB_1.5 min chromatography (MK RP18e 25-2 mm), MS (ESI) m/z 480.0 [M+H]+.
1-isopropyl-N-((1-(methylsulfonyl)piperidin-4-yl)methyl)isoindoline-5-carboxamide




Procedure same as that for N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropylisoindoline-5-carboxamide using tert-butyl 1-isopropyl-5-(((1-(methylsulfonyl)piperidin-4-yl)methyl)carbamoyl)isoindoline-2-carboxylate as the starting material. For some Examples, enantiomercially pure tert-butyl (S)-1-isopropyl-5-(((1-(methylsulfonyl)piperidin-4-yl)methyl)carbamoyl)isoindoline-2-carboxylate and tert-butyl (R)-1-isopropyl-5-(((1-(methylsulfonyl)piperidin-4-yl)methyl)carbamoyl)isoindoline-2-carboxylate were subjected to the same conditions to afford (S)-1-isopropyl-N-((1-(methylsulfonyl)piperidin-4-yl)methyl)isoindoline-5-carboxamide, respectively.
(1-(ethylsulfonyl)piperidin-4-yl)methanamine was prepared following the synthetic route shown in Scheme 15.




To a mixture of tert-butyl-(piperidin-4-ylmethyl)carbamate (500 mg, 2.34 mmol) and Et3N (1.18 g, 1.63 mL, 11.7 mmol) in CH2Cl2(8 mL) was added EtSO2Cl (602 mg, 4.68 mmol) at 0° C. The mixture was stirred at rt overnight. The mixture was added with CH2Cl2 (30 mL), washed with 1 N HCl (3×20 mL) and brine (20 mL). The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give tert-butyl ((1-(ethylsulfonyl)piperidin-4-yl)methyl)carbamate (700 mg, 98%) as a brown oil, which was used for the next step directly without further purification. 1H NMR (CDCl3 400 MHz): δ 4.64 (brs, 1H), 3.82 (d, J=12.4 Hz, 2H), 3.04 (t, J=6.4 Hz, 2H), 2.94 (q, J=7.6 Hz, 2H), 2.78 (t, J=10.0 Hz, 2H), 1.78 (d, J=12.8 Hz, 2H), 1.65-1.55 (m, 1H), 1.44 (s, 9H), 1.35 (t, J=7.6 Hz, 3H), 1.30-1.26 (m, 2H).
To a mixture of tert-butyl ((1-(ethylsulfonyl)piperidin-4-yl)methyl)carbamate (700 mg, 2.28 mmol) in dioxane (5 mL) was added HCl/dioxane (5 mL, 4N). The mixture was stirred at rt for 3 h. The mixture was concentrated under reduced pressure to give (1-(ethylsulfonyl)piperidin-4-yl)methanamine HCl salt (530 mg, 96%) as a slight yellow solid, which was used for the next step directly without further purification. 1H NMR (DMSO-d6 400 MHz): δ 8.05 (brs, 3H), 3.60-3.55 (m, 2H), 3.02 (q, J=7.6 Hz, 2H), 2.75-2.71 (m, 4H), 1.80-1.71 (m, 3H), 1.21-1.16 (m, 5H).
Tert-butyl 5-(((1-(ethylsulfonyl)piperidin-4-yl)methyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate




Procedure same as that for tert-butyl-5-(((5-(ethylsulfonyl)pyridin-2-yl)methyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate using (1-(ethylsulfonyl)piperidin-4-yl)methanamine HCl salt as the starting material.
N-((1-(ethylsulfonyl)piperidin-4-yl)methyl)-1-isopropylisoindoline-5-carboxamide




Procedure same as that for N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropylisoindoline-5-carboxamide using tert-butyl 5-(((1-(ethylsulfonyl)piperidin-4-yl)methyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate as the starting material.
(S)-3-amino-3-(4-(ethylsulfonyl)phenyl)propan-1-ol was prepared following the synthetic route shown in Scheme 16.




To a solution of (S)-methyl 3-((tert-butoxycarbonyl)amino)-3-(4-(ethylsulfonyl)phenyl)propanoate (650 mg, 1.75 mmol) in anhydrous MeOH/THF (1:3, 12 mL) was added LiBH4 (367 mg, 17.5 mmol). The mixture was stirred at rt overnight. The mixture was concentrated under reduced pressure and the residue was added to water (10 mL) and extracted with ethyl acetate (3×10 mL). The combined organic layers were washed with water (20 mL) then brine (20 mL), dried over anhydrous Na2SO4, filtered, concentrated and purified by preparative TLC (eluting with 75% ethyl acetate in petroleum ether) to afford (S)-tert-butyl (1-(4-(ethylsulfonyl)phenyl)-3-hydroxypropyl)carbamate (500 mg, 83%) as a white solid. LC-MS tR=0.610 min in 5-95 AB_1.5 min chromatography (MK RP-18e 25-2 mm), MS (ESI) m/z 365.9 [M+Na]+.
To a solution of (S)-tert-butyl (1-(4-(ethylsulfonyl)phenyl)-3-hydroxypropyl)carbamate (200 mg, 0.58 mmol) in CH3CN (1 mL), was added aq. 10% HCl (1 mL). The mixture was stirred at rt overnight. The mixture was concentrated under reduced pressure. To the crude residue was added a sat. K2CO3 solution to adjust to pH=9 and the product was extracted with ethyl acetate (3×5 mL). The combined organic layers were dried over anhydrous Na2SO4, filtered and concentrated to afford (S)-3-amino-3-(4-(ethylsulfonyl)phenyl)propan-1-ol (25 mg, 15%) as a yellow oil. The aqueous layer was lyophilized to afford a white solid, which was added to ethyl acetate (20 mL) and the mixture was stirred for 15 min. The mixture was then filtered and the filtrate was concentrated under reduced pressure to afford (S)-3-amino-3-(4-(ethylsulfonyl)phenyl)propan-1-ol (130 mg, 69%) as a yellow oil, which was used for the next step directly without further purification.
Tert-butyl 5-(((S)-1-(4-(ethylsulfonyl)phenyl)-3-hydroxypropyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate




Procedure same as that for tert-butyl-5-(((5-(ethylsulfonyl)pyridin-2-yl)methyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate using (S)-3-amino-3-(4-(ethylsulfonyl)phenyl)propan-1-ol as the starting material. Purification by preparative TLC afforded tert-butyl 5-(((S)-1-(4-(ethylsulfonyl)phenyl)-3-hydroxypropyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate as a yellow oil. A portion of this material was purified by SFC separation to afford pure enantiomers of tert-butyl (S)-5-(((S)-1-(4-(ethylsulfonyl)phenyl)-3-hydroxypropyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate and tert-butyl (R)-5-(((S)-1-(4-(ethylsulfonyl)phenyl)-3-hydroxypropyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate.
N—((S)-1-(4-(ethylsulfonyl)phenyl)-3-hydroxypropyl)-1-isopropylisoindoline-5-carboxamide




Procedure same as that for N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropylisoindoline-5-carboxamide, using tert-butyl 5-(((S)-1-(4-(ethylsulfonyl)phenyl)-3-hydroxypropyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate as the starting material. The crude product was used directly for the next step without further purification. For some Examples, pure enantiomers of both (S)-5-(((S)-1-(4-(ethylsulfonyl)phenyl)-3-hydroxypropyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate and tert-butyl (R)-5-(((S)-1-(4-(ethylsulfonyl)phenyl)-3-hydroxypropyl)carbamoyl)-1-isopropylisoindoline-2-carboxylate were subjected to the same conditions to give (S)—N—((S)-1-(4-(ethylsulfonyl)phenyl)-3-hydroxypropyl)-1-isopropylisoindoline-5-carboxamide and (R)—N—((S)-1-(4-(ethylsulfonyl)phenyl)-3-hydroxypropyl)-1-isopropylisoindoline-5-carboxamide, respectively.
(S)-3-amino-3-(5-(ethylsulfonyl)pyridin-2-yl)propan-1-ol (3:1 mixture of enantiomers S:R) was prepared following the synthetic route shown in Scheme 17.




A mixture of 3-((tert-butyldimethylsilyl)oxy)propanal (10 g, 53.1 mmol), (R)-2-methylpropane-2-sulfinamide (7.72 g, 63.7 mmol) and CuSO4 (21.2 g, 13.3 mmol) in CH2Cl2 (100 mL) was stirred at rt for 17 h. The mixture was filtered and the filtrate was concentrated under reduced pressure. The residue was purified by column chromatography on silica gel (eluting with petroleum ether:ethyl acetate=20:1) to give (R,E)-N-(3-((tert-butyldimethylsilyl)oxy)propylidene)-2-methylpropane-2-sulfinamide (7.2 g, 48%) as a yellow oil. LC-MS tR=0.900 min in 5-95AB_1.5 min chromatography (RP-18e, 25-2 mm), MS (ESI) m/z 292.1 [M+H]+.
To toluene (120 mL) was added n-BuLi (10 mL, 24.0 mmol) at −70° C. under a N2 atmosphere, followed by the dropwise addition of a solution of 2-bromo-5-(ethylthio)pyridine (5.8 g, 26.4 mmol) in toluene (15 mL). After being stirred for 30 min, a solution of (R,E)-N-(3-((tert-butyldimethylsilyl)oxy)propylidene)-2-methylpropane-2-sulfinamide (7.0 g, 24.0 mmol) in toluene (15 mL) was added dropwise at −70° C. The resulting mixture was stirred at −70° C. for 2 h and slowly allowed to warm to rt, at which point the stirring was continued for an additional 18 h. To the mixture was added a sat. aq. NH4Cl solution (20 mL) slowly below −40° C. and the product was extracted with ethyl acetate (3×50 mL). The combined organic layers were washed with brine (50 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica gel (eluting with petroleum ether:ethyl acetate=gradient from 10:1 to 3:1) to give a 3:1 mixture of (R)—N—((S)-3-((tert-butyldimethylsilyl)oxy)-1-(5-(ethylthio)pyridine-2-yl)propyl)-2-methylpropane-2-sulfinamide and (R)—N—((R)-3-((tert-butyldimethylsilyl)oxy)-1-(5-(ethylthio)pyridine-2-yl)propyl)-2-methylpropane-2-sulfinamide, respectively, (5.1 g, 49%) as a yellow oil. This 3:1 mixture of diastereomers was used for the subsequent reactions. LC-MS tR=1.313 min in 10-80AB_2.0 min chromatography (Xtimate 3 um, C18, 2.1*30 mm), MS (ESI) m/z 431.5 [M+H]+.
To a solution of (R)—N—((S)-3-((tert-butyldimethylsilyl)oxy)-1-(5-(ethylthio)pyridine-2-yl)propyl)-2-methylpropane-2-sulfinamide (5.1 g, 11.84 mmol), as a 3:1 mixture of diastereomers from the previous step, in CH2Cl2 (40 mL) was added HCl/dioxane (10 mL, 4N) at 0° C. The mixture was stirred at 21-24° C. for 17 h. The residue was concentrated under reduced pressure to afford (S)-3-amino-3-(5-(ethylthio)pyridin-2-yl)propan-1-ol (2.5 g, crude) as a 3:1 mixture of enantiomers (S:R), which was used for the next step directly without further purification.
To a solution of (S)-3-amino-3-(5-(ethylthio)pyridin-2-yl)propan-1-ol (2.5 g, 11.8 mmol), as a 3:1 mixture of enantiomers (S:R), in MeOH (10 mL) was added dropwise Oxone® monopersulfate (1.5 g, 2.47 mmol) in H2O (10 mL) at 0° C. The mixture was stirred at 22-25° C. for 2 h at which point LCMS analysis showed complete consumption of the starting material. The mixture was filtered and concentrated under reduced pressure to remove MeOH. The residue was purified by basic preparative HPLC method to give (S)-3-amino-3-(5-(ethylsulfonyl)pyridin-2-yl)propan-1-ol (300 mg, 10%) as a 3:1 mixture of enantiomers (S:R). This 3:1 mixture of enantiomers was used for the subsequent reactions. LC-MS tR=1.289 min in 0-30CD_3.0 min chromatography (Xtimate ODS 2.1*30 mm, 3 um), MS (ESI) m/z 245.0 [M+1]+. 1H NMR (CDCl3 400 MHz): δ 9.05 (d, J=2.0 Hz, 1H), 8.16 (dd, J=8.0, 2.4 Hz, 1H), 7.49 (d, J=8.0 Hz, 1H), 4.33-4.36 (m, 2H), 3.83-3.92 (m, 2H), 3.17 (q, J=7.2 Hz, 2H), 1.91-1.94 (m, 2H), 1.33 (t, J=7.6 Hz, 3H).
Tert-butyl (S)-1-ethyl-5-(((S)-1-(5-(ethylsulfonyl)pyridin-2-yl)-3-hydroxypropyl)carbamoyl)isoindoline-2-carboxylate




Procedure same as that for (S)-tert-butyl 1-ethyl-5-(((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)carbamoyl)isoindoline-2-carboxylate using (S)-3-amino-3-(5-(ethylsulfonyl)pyridin-2-yl)propan-1-ol (3:1 mixture of enantiomers) as the starting material. LCMS tR=1.061 min in 10-80AB_2.0 min chromatography (Xtimate 3 um, C18, 2.1*30 mm), MS (ESI) m/z 518.1 [M+H]+.
(S)-1-ethyl-N—((S)-1-(5-(ethylsulfonyl)pyridin-2-yl)-3-hydroxypropyl)isoindoline-5-carboxamide




Procedure same as that for (S)-1-ethyl-N—((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)isoindoline-5-carboxamide using tert-butyl (S)-1-ethyl-5-(((S)-1-(5-(ethylsulfonyl)pyridin-2-yl)-3-hydroxypropyl)carbamoyl)isoindoline-2-carboxylate as the starting material.
4-(aminomethyl)-N-methylbenzenesulfonamide was prepared following the synthetic route shown in Scheme 18.




To a solution of MeNH2 (2M, 25.3 mL, 50 mmol) and DMAP (0.5 g, 4.0 mmol) in dichloromethane (50 mL) was added 4-cyanobenzene-1-sulfonyl chloride (4.0 g, 20 mmol) at 0° C. The mixture was stirred at rt for 2 h. TLC analysis (eluting with petroleum ether:ethyl acetate=1:1) showed the reaction was complete. The mixture was acidified to pH=1 with HCl (2N) and extracted with dichloromethane (2×50 mL). The combined organic layers were dried over anhydrous Na2SO4, filtered and concentrated in vacuo to give 4-cyano-N-methylbenzenesulfonamide (3.8 g, 97%) as a white solid. 1H NMR (CDCl3 400 MHz): δ 7.98 (d, J=8.4 Hz, 2H), 7.84 (d, J=8.4 Hz, 2H), 4.51-4.60 (m, 1H), 2.71 (d, J=5.2 Hz, 3H).
To a solution of 4-cyano-N-methylbenzenesulfonamide (3.8 g, 19.4 mmol) in CH3OH (60 mL) was added PtO2 (1.2 g, w/w 30%). The mixture was stirred under H2 (30 psi) at rt for 16 h. The mixture was filtered and the filtrate was concentrated under reduced pressure to give 4-(aminomethyl)-N-methylbenzenesulfonamide (3.7 g, 95%) as oil. 1H NMR (CD3OD 400 MHz): δ 7.78 (d, J=8.0 Hz, 2H), 7.54 (t, J=8.4 Hz, 2H), 3.85 (d, J=7.2 Hz, 2H), 2.48-2.51 (m, 3H).
Tert-butyl (S)-1-ethyl-5-((4-(N-methylsulfamoyl)benzyl)carbamoyl)isoindoline-2-carboxylate




Procedure same as that for (S)-tert-butyl 1-ethyl-5-(((5-(ethylsulfonyl)pyridin-2-yl)methyl)carbamoyl)isoindoline-2-carboxylate using (S)-2-(tert-butoxycarbonyl)-1-ethylisoindoline-5-carboxylic acid and 4-(aminomethyl)-N-methylbenzenesulfonamide as the starting materials.
(S)-1-ethyl-N-(4-(N-methylsulfamoyl)benzyl)isoindoline-5-carboxamide




Procedure same as that for (S)-1-ethyl-N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)isoindoline-5-carboxamide using tert-butyl (S)-1-ethyl-5-((4-(N-methylsulfamoyl)benzyl)carbamoyl)isoindoline-2-carboxylate as the starting material.
6-(aminomethyl)-N-methylpyridine-3-sulfonamide was prepared following the synthetic route shown in Scheme 19.




To 9 mL of H2O was added SOCl2 (2.7 mL, 23.1 mmol) at 0° C. Then the mixture was stirred at rt overnight. To the above mixture was added CuCl (8 mg, 0.08 mmol) and the resulting mixture was cooled to −5° C. To a mixture of 5-aminopicolinonitrile (500 mg, 4.2 mmol) in HCl (conc., 5 mL) was added a solution of NaNO2 (410 mg, 6.3 mmol) in H2O (3 mL) dropwise at −5° C. and the mixture was stirred at −5° C. for 1 h. Then, the mixture was added to the above SOCl2/H2O/CuCl mixture and stirred for 1 h. A red solid precipitated during addition. The precipitate was collected by filtration, washed with cold water and dried in vacuo to give 6-cyanopyridine-3-sulfonyl chloride (800 mg, 94%) as a red solid, which was used directly for the next step without further purification. LCMS tR=1.354 min in 0-30AB_2.0 min chromatography (Xtimate C18, 2.1*30 mm, 3 um), MS (ESI) m/z 202.9 [M+H]+.
To a solution of MeNH2 (2M, 2.5 mL, 5 mmol) and DMAP (50 mg, 0.4 mmol) in dichloromethane (5 mL) was added 6-cyanopyridine-3-sulfonyl chloride (0.4 g, 2.0 mmol) at 0° C. The mixture was stirred at rt for 2 h. TLC analysis (eluting petroleum ether:ethyl acetate=1:1) showed the reaction was complete. The mixture was acidified to pH=1 with HCl (2N) and extracted with dichloromethane (2×5 mL). The combined organic layers were dried over anhydrous Na2SO4, filtered and concentrated in vacuo to give 6-cyano-N-methylpyridine-3-sulfonamide (290 mg, 74%) as a red solid. LCMS tR=0.426 min in 5-95AB_1.5 min chromatography (MK RP18e 25-2 mm), MS (ESI) m/z 197.9 [M+H]+.
To a solution of 6-cyano-N-methylpyridine-3-sulfonamide (200 mg, 1.0 mmol) in CH3OH (5 mL) was added PtO2 (50 mg, w/w 25%). The mixture was stirred under H2 (30 psi) at rt overnight. The mixture was filtered and the filtrate was concentrated under reduced pressure to give 6-(aminomethyl)-N-methylpyridine-3-sulfonamide (200 mg, 100%) as an oil. 1H NMR (CDCl3 400 MHz): δ 9.00 (d, J=2.4 Hz, 1H), 8.10 (dd, J=8.0, 2.4 Hz, 1H), 7.50 (d, J=8.4 Hz, 1H), 4.10 (s, 2H), 2.71 (s, 3H).
Tert-butyl (S)-1-ethyl-5-(((5-(N-methylsulfamoyl)pyridin-2-yl)methyl)carbamoyl)isoindoline-2-carboxylate




Procedure same as that for (S)-tert-butyl 1-ethyl-5-(((5-(ethylsulfonyl)pyridin-2-yl)methyl)carbamoyl)isoindoline-2-carboxylate using (S)-2-(tert-butoxycarbonyl)-1-ethylisoindoline-5-carboxylic acid and 6-(aminomethyl)-N-methylpyridine-3-sulfonamide as the starting materials. LCMS tR=0.779 min in 5-95AB_1.5 min chromatography (RP-18e,25-2 mm), MS (ESI) m/z 475.0 [M+H]+.
(S)-1-ethyl-N-((5-(N-methylsulfamoyl)pyridin-2-yl)methyl)isoindoline-5-carboxamide




Procedure same as that for (S)-1-ethyl-N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)isoindoline-5-carboxamide using tert-butyl (S)-1-ethyl-5-(((5-(N-methylsulfamoyl)pyridin-2-yl)methyl)carbamoyl)isoindoline-2-carboxylate as the starting material.
Preparation of Compounds of Formula I
Trans-4-(trifluoromethyl)cyclohexane-1-carbaldehyde (was prepared following the synthetic route shown in Scheme 20.




To a solution of trans-4-(trifluoromethyl)cyclohexane carboxylic acid (789 mg, 4.02 mmol) in THF (12 mL) at rt was added lithium aluminum hydride (1.0 M in THF, 4.02 mL). The mixture was heated to reflux and stirred for 3 h. It was then cooled to 0° C. and quenched successively with water (152 μL), 15% aqueous sodium hydroxide (152 μL), and water (456 μL). The mixture was then filtered through Celite and concentrated under reduced pressure. The crude liquid (trans-4-(trifluoromethyl)cyclohexyl)methanol was carried forward without any purification and without placing under high vacuum due to its volatility.
To a solution of oxalyl chloride (6.2 mL, 87.4 mmol) in anhydrous CH2Cl2 (300 mL) was added dropwise DMSO (12.5 mL, 0.17 mol) at −78° C. under N2. After the mixture was stirred at −78° C. for 30 min., a solution of (trans-4-(trifluoromethyl)cyclohexyl)methanol (5.3 g, 29.1 mmol) in CH2Cl2 (40 mL) was added dropwise while keeping the internal temperature below −65° C. After being stirred for 30 min., a solution of Et3N (40.5 mL, 0.29 mol) in CH2Cl2 (60 mL) was added dropwise slowly, keeping the internal temperature below −65° C. The reaction mixture was stirred at −78° C. for 1 h, and warmed to rt overnight. The mixture was washed with water (3×300 mL) and brine (300 mL), dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica (eluting with 15% EtOAc in petroleum ether) to give trans-4-(trifluoromethyl)cyclohexane-1-carbaldehyde (4.6 g, 87%) as a yellow oil.
Examples 1a and 1b
N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropyl-2-((trans-4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxamide (1a) and (1b)




Examples 1a and 1b were prepared according to General Procedure A outlined below.

General Procedure A:





A mixture of trans-4-(trifluoromethyl)cyclohexanecarbaldehyde (1.3 g, 7.235 mmol) and N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropylisoindoline-5-carboxamide (560 mg, 1.447 mmol) in anhydrous MeOH (10 mL) was adjusted to pH=6 with AcOH. NaBH3CN (273 mg, 4.341 mmol) was added. The mixture was stirred at 70° C. for 4.5 h before being concentrated under reduced pressure. Water (15 mL) was added and the aqueous layer was extracted with ethyl acetate (3×15 mL). The combined organic layers were washed with brine (45 mL), dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica gel (eluting with 25% ethyl acetate in petroleum ether followed by SFC separation (AD-3) and HCl preparative HPLC method D separation to afford HCl salts of isomer 1 (1a) (175.9 mg, 21%) and isomer 2 (1b) (188.8 mg, 22%) as white solids.
Isomer 1 (1a) HCl salt (175.90 mg, 21%) LC-MS tR=0.693 min in 5-95AB_1.5 min chromatography (Welch MK RP-18e 25-2 mm), MS (ESI) m/z 552.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 9.08 (d, J=2.0 Hz, 1H), 8.33 (dd, J=2.4, 8.4 Hz, 1H), 8.00 (d, J=8.0 Hz, 1H), 7.98 (s, 1H), 7.72 (d, J=8.4 Hz, 1H), 7.58 (d, J=8.0 Hz, 1H), 5.11 (d, J=15.2 Hz, 1H), 4.99 (d, J=4.8 Hz, 1H), 4.83 (s, 2H), 4.65 (d, J=15.2 Hz, 1H), 3.20 (q, J=7.2 Hz, 2H), 3.26-3.15 (m, 2H), 2.48-2.34 (m, 1H), 2.25-1.90 (m, 6H), 1.50-1.35 (m, 2H), 1.33-1.28 (m, 2H), 1.26 (t, J=7.2 Hz, 3H), 1.21 (d, J=6.8 Hz, 3H), 1.04 (d, J=6.8 Hz, 3H). Isomer SFC tR=6.938 min in 12 min chromatography (ee=100%).
Isomer 2 (1b) HCl salt (188.80 mg, 22%) LC-MS tR=0.631 min in 5-95AB_1.5 min chromatography (Welch MK RP-18e 25-2 mm), MS (ESI) m/z 552.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 9.08 (d, J=2.0 Hz, 1H), 8.33 (dd, J=2.0, 8.0 Hz, 1H), 8.02 (d, J=8.0 Hz, 1H), 7.99 (s, 1H), 7.70 (d, J=8.4 Hz, 1H), 7.60 (d, J=8.0 Hz, 1H), 5.11 (dd, J=4.0, 15.6 Hz, 1H), 4.99 (d, J=4.4 Hz, 1H), 4.83 (s, 2H), 4.67 (dd, J=5.2, 15.2 Hz, 1H), 3.31 (q, J=7.2 Hz, 2H), 3.26-3.15 (m, 2H), 2.48-2.38 (m, 1H), 2.25-1.90 (m, 6H), 1.52-1.35 (m, 2H), 1.33-1.29 (m, 2H), 1.27 (t, J=7.2 Hz, 3H), 1.23 (d, J=6.8 Hz, 3H), 1.06 (d, J=6.8 Hz, 3H). Isomer SFC tR=9.651 min in 12 min chromatography (ee=97.96%).
The following compounds in Table 1 were prepared using General Procedure A described above with trans-4-(trifluoromethyl)cyclohexane-1-carbaldehyde and the appropriate intermediates described herein.










TABLE 1





Ex.





No.
Structure
LCMS
1H-NMR


















2a




582.0 [M + H]+
(CD3OD 400 MHz): δ 9.07 (d, J = 2.0 Hz, 1H), 8.51 (dd, J = 2.0, 8.4 Hz, 1H), 7.99-7.92 (m, 3H), 7.51 (d, J = 8.4 Hz, 1H), 5.33 (t, J = 6.4 Hz, 1H), 5.10-5.05 (m, 1H), 4.97-4.92 (m, 1H), 4.58-4.53 (m, 1H), 4.07-4.02 (m, 2H), 3.31 (q, J = 7.2 Hz, 2H), 3.20-3.10 (m, 2H), 2.40-2.30 (m, 1H), 2.20-1.90 (m, 6H), 1.44-1.28 (m, 2H), 1.23 (t, J = 7.2 Hz, 3H), 1.25-1.16 (m, 2H), 1.15 (d, J = 6.8 Hz, 3H), 0.98 (d, J = 6.8 Hz, 3H)


 


2b




582.0 [M + H]+
(CD3OD 400 MHz): δ 9.07 (s, 1H), 8.54 (d, J = 8.0 Hz, 1H), 8.03-7.87 (m, 3H), 7.49 (d, J = 7.6 Hz, 1H), 5.32 (t, J = 5.6 Hz, 1H), 5.10- 5.05 (m, 1H), 4.97-4.92 (m, 1H), 4.58-4.53 (m, 1H), 4.07-4.00 (m, 2H), 3.30 (q, J = 7.2 Hz, 2H), 3.20-3-10 (m, 2H), 2.40-2.30 (m, 1H), 2.20-1.85 (m, 6H), 1.42-1.30 (m, 2H), 1.21 (t, J = 7.2 Hz, 3H), 1.20-1.14 (m, 2H), 1.13 (d, J = 6.8 Hz, 3H), 0.96 (d, J = 6.8 Hz, 3H)


 


2c




582.0 [M + H]+
(CD3OD 400 MHz): δ 9.04 (s, 1H), 8.55-8.45 (m, 1H), 8.01-7.90 (m, 3H), 7.47 (d, J = 8.4 Hz, 1H), 5.30 (t, J = 5.6 Hz, 1H), 5.08-5.02 (m, 1H), 4.97-1.92 (m, 1H), 4.58-1.53 (m, 1H), 4.07-4.00 (m, 2H), 3.27 (q, J = 7.2 Hz, 2H), 3.17-3.10 (m, 2H), 2.41-2.30 (m, 1H), 2.13-1.81 (m, 6H), 1.40-1.30 (m, 2H), 1.18 (t, J = 7.2 Hz, 3H), 1.17-1.13 (m, 2H), 1.11 (d, J = 6.8 Hz, 3H), 0.94 (d, J = 6.8 Hz, 3H)


 


2d




582.0 [M + H]+
(CD3OD 400 MHz): δ 9.01 (s, 1H), 8.60-8.45 (m, 1H), 8.04-7.85 (m, 3H), 7.41 (d, J = 8.0 Hz, 1H), 5.25 (t, J = 5.6 Hz, 1H), 5.08-4.98 (m, 1H), 4.97-4.92 (m, 1H), 4.55-4.48 (m, 1H), 4.03-3.95 (m, 2H), 3.30-3.20 (m, 2H), 3.15-3.08 (m, 2H), 2.35-2.20 (m, 1H), 2.13- 1.81 (m, 6H), 1.40-1.20 (m, 2H), 1.13 (t, J = 7.2 Hz, 3H), 1.12-1.08 (m, 2H), 1.05 (d, J = 6.8 Hz, 3H), 0.89 (d, J = 6.8 Hz, 3H).


 


3a




581.1 (M + H)+
(CD3OD 400 MHz): δ 8.99 (d, J = 8.0 Hz, 1H), 8.00 (d, J = 7.6 Hz, 1H), 7.97 (s, 1H), 7.92 (d, J = 8.4 Hz, 2H), 7.71 (d, J = 8.8 Hz, 2H), 7.57 (d, J = 8.0 Hz, 1H), 5.30 (q, J = 6.8 Hz, 1H), 5.15-5.07 (m, 1H), 5.05-4.98 (m, 1H), 4.68-4.59 (m, 1H), 3.94 (d, J = 6.4 Hz, 2H), 3.25-3.16 (m, 4H), 2.50-2.30 (m, 1H), 2.25-1.95 (m, 6H), 1.55-1.35 (m, 2H), 1.35- 1.23 (m, 6H), 1.23 (t, J = 7.2 Hz, 3H), 1.05 (d, J = 6.8 Hz, 3H).


 


3b




581.1 (M + H)+
(CD3OD 400 MHz): δ 8.98 (d, J = 7.6 Hz, 1H), 8.02 (d, J = 8.0 Hz, 1H), 7.96 (s, 1H), 7.92 (d, J = 8.4 Hz, 2H), 7.71 (d, J = 8.4 Hz, 2H), 7.58 (d, J = 7.2 Hz, 1H), 5.30 (q, J = 6.8 Hz, 1H), 5.15-5.07 (m, 1H), 5.05-4.98 (m, 1H), 4.68-4.59 (m, 1H), 3.93 (d, J = 6.8 Hz, 2H), 3.25-3.15 (m, 4H), 2.50-2.35 (m, 1H), 2.25-1.90 (m, 6H), 1.55-1.36 (m, 2H), 1.35- 1.23 (m, 6H), 1.23 (1, J = 7.2 Hz, 3H), 1.05 (d, J = 6.4 Hz, 3H).


 


3c




581.1 (M + H)+
(CD3OD 400 MHz): δ 7.99 (d, J = 8.4 Hz, 1H), 7.92 (s, 1H), 7.89 (d, J = 8.0 Hz, 2H), 7.68 (d, J = 8.4 Hz, 2H), 7.56 (d, J = 6.8 Hz, 1H), 5.27 (t, J = 6.8 Hz, 1H), 5.12-5.04 (m, 1H), 5.02-4.98 (m, 1H), 4.65-4.58 (m, 1H), 3.93-3.86 (m, 2H), 3.25-3.15 (m, 2H), 3.19 (q, J = 7.2 Hz, 2H), 2.38-2.35 (m, 1H), 2.20- 1.90 (m, 6H), 1.55-1.36 (m, 2H), 1.35-1.23 (m, 6H), 1.23 (t, J = 7.2 Hz, 3H), 1.02 (d, J = 6.4 Hz, 3H).


 


3d




581.1 (M + H)+
(OD3OD 400 MHz): δ 9.00-8.97 (m, 1H), 8.00 (d, J = 8.0 Hz, 1H), 7.96 (s, 1H), 7.92 (d, J = 8.4 Hz, 2H), 7.71 (d, J = 8.4 Hz, 2H), 7.58 (d, J = 8.0 Hz, 1H), 5.30 (t, J = 6.5 Hz, 1H), 5.12-5.07 (m, 1H), 5.03-4.99 (m, 1H), 4.68-4.62 (m, 1H), 3.95-3.90 (m, 2H), 3.26- 3.19 (m, 2H), 3.22 (q, J = 7.2 Hz, 4H), 2.45- 2.38 (m, 1H), 2.20-1.90 (m, 6H), 1.51-1.36 (m, 2H), 1.35-1.23 (m, 6H), 1.23 (t, J = 7.2 Hz, 3H), 1.05 (d, J = 6.8 Hz, 3H).


 


4a




544.1 (M + H)+
(CD3OD 400 MHz): δ 7.91 (d, J = 7.6 Hz, 1H), 7.90 (s, 1H), 7.55 (d, J = 8.4 Hz, 1H), 5.09 (d, J = 15.2 Hz, 1H), 5.05-4.95 (m, 3H), 4.63 (d, J = 15.2 Hz, 1H), 3.74 (d, J = 11.6 Hz, 2H), 3.40-3.36 (m, 2H), 3.30-3.15 (m, 2H), 2.82 (s, 3H), 2.75-2.65 (m, 2H), 2.45- 2.33 (m, 1H), 2.25-1.70 (m, 8H), 1.50-1.25 (m, 5H), 1.21 (d, J = 6.8 Hz, 3H), l.03 (d, J = 6.4 Hz, 3H).


 


4b




544.1 (M + H)+
(CD3OD 400 MHz): δ 8.68 (brs, 1H), 7.88 (s, 2H), 7.53 (d, J = 7.6 Hz, 1H), 5.07 (d, J = 15.2 Hz, 1H), 5.02-4.95 (m, 3H), 4.61 (d, J = 15.2 Hz, 1H), 3.73 (d, J = 12.0 Hz, 2H), 3.40-3.36 (m, 2H), 3.27-3.13 (m, 2H), 2.81 (s, 3H), 2.77-2.65 (m, 2H), 2.45-2.33 (m, 1H), 2.25-1.70 (m, 8H), 1.50-1.20 (m, 5H), 1.19 (d, J = 6.4 Hz, 3H), 1.02 (d, J = 6.41 Hz, 3H).


 


5a




558.1 (M + H)+
(CD3OD 400 MHz): δ 8.69 (brs, 1H), 7.88 (s, 2H), 7.53 (d, J = 7.6 Hz, 1H), 5.07 (d, J = 15.2 Hz, 1H), 5.03-4.95 (m, 3H), 4.61 (d, J = 14.8 Hz, 1H), 3.75 (d, J = 12.0 Hz, 2H), 3.45-3.35 (m, 2H), 3.30-3.15 (m, 2H), 3.01 (q, J = 7.2 Hz, 2H), 2.83 (t, J = 10.8 Hz, 2H), 2.45-2.33 (m, 1H), 2.25-1.75 (m, 8H), 1.50- 1.40 (m, 3H), 1.30 (t, J = 7.2 Hz, 3H), 1.28- 1.22 (m, 2H), 1.19 (d, J = 6.8 Hz, 3H), 1.02 (d, J = 6.4 Hz, 3H).


 


5b




558.1 (M + H)+
(CD3OD 400 MHz): δ 8.69 (brs, 1H), 7.88 (s, 2H), 7.53 (d, J = 8.0 Hz, 1H), 5.07 (d, J = 15.6 Hz, 1H), 5.03-4.93 (m, 3H), 4.61 (d, J = 15.6 Hz, 1H), 3.76 (d, J = 12.4 Hz, 2H), 3.45-3.34 (m, 2H), 3.30-3.15 (m, 2H), 3.01 (q, J = 7.2 Hz, 2H), 2.83 (t, J = 11.6 Hz, 2H), 2.45-2.32 (m, 1H), 2.25-1.75 (m, 8H), 1.45- 1.35 (m, 3H), 1.30 (t, J = 7.2 Hz, 3H), 1.28- 1.22 (m, 2H), 1.19 (d, J = 6.4 Hz, 3H). 1.02 (d, J = 6.4 Hz, 3H).


 


6a




595.2 (M + H)+
(CD3OD 400 MHz): δ 9.06 (d, J = 7.6 Hz, 1H), 7.93 (d, J = 8.0 Hz, 1H), 7.89 (s, 1H), 7.88 (d, J = 8.0 Hz, 2H), 7.67 (d, J = 8.4 Hz, 2H), 7.54 (d, J = 8.0 Hz, 1H ), 5.39-5.34 (m, 1H), 5.06 (d, J = 15.2 Hz, 1H), 4.95 (d, J = 4.8 Hz, 1H), 4.60 (d, J = 15.2 Hz, 1H), 3.67- 3.61 (m, 2H), 3.22-3.18 (m, 2H), 3.19 (q, J = 7.2 Hz, 2H), 2.38-2.36 (m, 1H), 2.17-1.98 (m, 8H), 1.42-1.39 (m, 2H), 1.23-1.19 (m, 2H), 1.20 (t, J = 7.2 Hz, 3H), 1.19 (d, J = 7.2 Hz, 3H), 1.01 (d, J = 6.4 Hz, 3H)


 


6b




595.2 (M + H)+
(CD3OD 400 MHz): δ 7.92 (d, J = 8.0 Hz, 1H), 7.89 (s, 1H), 7.87 (d, J = 8.4 Hz, 2H), 7.67 (d, J = 8.4 Hz, 2H), 7.54 (d, J = 8.0 Hz, 1H), 5.38-5.35 (m, 1H), 5.06 (d, J = 15.2 Hz, 1H), 4.95 (d, J = 4.8 Hz, 1H), 4.60 (d, J = 15.2 Hz, 1H), 3.67-3.60 (m, 2H), 3.23-3.19 (m, 2H), 3.18 (q, J = 7.2 Hz, 2H), 2.38-2.36 (m, 1H), 2.16-1.94 (m, 8H), 1.42-1.39 (m, 2H), 1.23-1.19 (m, 2H), 1.20 (t, J = 7.2 Hz, 3H), 1.18 (d, J = 7.2 Hz, 3H), 1.01 (d, J = 6.8 Hz, 3H).









Examples 7a and 7b
2-((trans-4-ethoxycyclohexyl)methyl)-N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropylisoindoline-5-carboxamide (7a) and (7b)




To a solution of trans-methyl 4-hydroxycyclohexanecarboxylate (1.0 g, 6.32 mmol) in anhydrous DMF (10 mL) was added NaH (759 mg, 18.96 mmol, 60% in mineral oil) in portions at 0° C. under N2. The mixture was stirred at 0° C. for 30 min and EtI (4.93 g, 31.6 mmol) was then added at 0° C. The mixture was allowed to warm to rt and stirred for another 1.5 h. TLC analysis (petroleum ether:ethyl acetate=5:1) showed the starting material was consumed. The mixture was quenched with a sat. NH4Cl solution (50 mL) at 0° C. and extracted with MTBE (3×20 mL). The combined organic layers were washed with H2O (15 mL) then brine (15 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to afford trans-methyl 4-ethoxycyclohexanecarboxylate (680 mg, 58%) as a colorless liquid, which was used for next step directly without purification.
To a solution of trans-methyl 4-ethoxycyclohexanecarboxylate (680 mg, 3.65 mmol) in anhydrous THF (10 mL) was added LiAlH4 (5.5 mL, 10.95 mmol, 2 M in THF) dropwise at 0° C. The mixture was stirred at rt for 2 h. TLC analysis (petroleum ether:ethyl acetate=2:1) showed the starting material was consumed. The mixture was quenched with water (0.4 mL) slowly followed by a 10% aqueous NaOH solution (0.4 mL) at 0° C. The mixture was filtered and the filtrate was concentrated under reduced pressure. The residue was purified by chromatography column on silica gel to afford (trans-4-ethoxycyclohexyl)methanol (210 mg, 36%) as a colorless oil. 1H NMR (CDCl3 400 MHz): δ 3.51 (q, J=7.2 Hz, 2H), 3.45 (d, J=6.8 Hz, 2H), 3.20-3.15 (m, 1H), 2.10-2.06 (m, 2H), 1.86-1.83 (m, 2H), 1.45-1.25 (m, 3H), 1.20 (t, J=6.8 Hz, 3H), 1.00-0.96 (m, 2H).
To a solution of (trans-4-ethoxycyclohexyl)methanol (60 mg, 0.38 mmol) in CH2Cl2 (2 mL) was added PCC (163 mg, 0.76 mmol). The mixture was stirred at rt for 1.5 h. TLC analysis (petroleum ether:ethyl acetate=3:1) showed the starting material was consumed. Water (15 mL) was added to the mixture and the product was extracted with CH2Cl2 (3×15 mL). The combined organic layers were washed with water (3×10 mL) then brine (10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to afford crude trans-4-ethoxycyclohexanecarbaldehyde (crude 60 mg, 100%) as a pink oil, which was used for the next step directly without further purification.
Isomer 1 (7a) was made according to General Procedure A using trans-4-ethoxycyclohexane-1-carbaldehyde and (S)—N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropylisoindoline-5-carboxamide as the starting materials. The crude material was purified by HCl preparative HPLC method E separation to afford isomer 1 HCl salt (7a) (3.10 mg, 9%) as a white solid. LC-MS tR=1.207 min in 0-60AB_2 min chromatography (Xtimate C18 2.1*30 mm), MS (ESI) m/z 528.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 9.05 (s, 1H), 8.43 (d, J=8.4 Hz, 1H), 8.00 (d, J=9.2 Hz, 1H), 7.99 (s, 1H), 7.81 (d, J=8.4 Hz, 1H), 7.58 (d, J=8.0 Hz, 1H), 5.10 (d, J=15.2 Hz, 1H), 4.98 (d, J=5.2 Hz, 1H), 4.86 (s, 2H), 4.64 (d, J=15.6 Hz, 1H), 3.56 (q, J=7.2 Hz, 2H), 3.36-3.34 (m, 2H), 3.23-3.21 (m, 3H), 2.43-2.39 (m, 1H), 2.15-2.13 (m, 2H), 2.14-2.01 (m, 1H), 1.93-1.90 (m, 2H), 1.29-1.16 (m, 13H), 1.04 (d, J=6.8 Hz, 3H). Isomer SFC tR=4.350 min in 12 min chromatography (ee=98.2%).
Isomer 2 (7b) was made according to General Procedure A using trans-4-ethoxycyclohexane-1-carbaldehyde and (R)—N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropylisoindoline-5-carboxamide as the starting materials. The crude material was purified by HCl preparative HPLC Method E separation to afford the HCl salt of isomer 2 (7b) (8.10 mg, 23%) as a white solid. LC-MS tR=0.686 min in 5-95AB_1.5 min chromatography (MK RP Xtimate C18 2.1*30 mm), MS (ESI) m/z 528.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 9.06 (s, 1H), 8.44 (d, J=8.0 Hz, 1H), 8.00 (d, J=9.2 Hz, 1H), 7.99 (s, 1H), 7.83 (d, J=8.4 Hz, 1H), 7.57 (d, J=7.6 Hz, 1H), 5.11 (d, J=14.8 Hz, 1H), 4.99 (d, J=4.4 Hz, 1H), 4.86 (s, 3H), 4.64 (d, J=15.6 Hz, 1H), 3.56 (q, J=6.8 Hz, 2H), 3.36-3.33 (m, 2H), 3.23-3.20 (m, 2H), 2.43-2.35 (m, 1H), 2.12-2.02 (m, 3H), 1.95-1.93 (m, 2H), 1.30-1.20 (m, 12H), 1.04 (d, J=7.2 Hz, 3H).
Examples 8a and 8b
(S)-2-((-4-ethoxycyclohexyl)methyl)-N—((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)-1-isopropylisoindoline-5-carboxamide (8a) and (8b)




Isomers 1 and 2 (8a and 8b) was made according to General Procedure A using trans-4-ethoxycyclohexane-1-carbaldehyde and (S)—N—((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)-1-isopropylisoindoline-5-carboxamide as the starting materials. The crude residue was purified by HCl preparative HPLC Method E separation to afford the HCl salts of isomer 1 (8a) (6.90 mg, 10%) and isomer 2 (8b) (6.80 mg, 10%) as white solids.
Isomer 1 (8a) (6.90 mg, 10%). LC-MS tR=0.652 min in 5-95AB_1.5 min chromatography (Welch MERCK RP-18e 25-2 mm), MS (ESI) m/z 557.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 8.04 (d, J=8.0 Hz, 1H), 8.00 (s, 1H), 7.95 (d, J=8.0 Hz, 2H), 7.75 (d, J=8.4 Hz, 2H), 7.60 (d, J=8.0 Hz, 1H), 5.34 (t, J=6.4 Hz, 1H), 5.17-5.10 (m, 1H), 5.05-5.01 (m, 1H), 4.71-4.64 (m, 1H), 3.98 (d, J=6.4 Hz, 2H), 3.61 (q, J=7.2 Hz, 2H), 3.37-3.31 (m, 1H), 3.30-3.22 (m, 2H), 3.25 (q, J=7.2 Hz, 2H), 2.51-2.40 (m, 1H), 2.25-1.94 (m, 5H), 1.45-1.20 (m, 13H), 1.09 (d, J=6.8 Hz, 3H). Isomer SFC tR=2.113 min in 3 min chromatography (ee=97.92%). Isomer 2 (8b) (6.80 mg, 10%). LC-MS tR=0.661 min in 5-95AB_1.5 min chromatography (Welch MERCK RP-18e 25-2 mm), MS (ESI) m/z 557.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 8.03 (d, J=7.6 Hz, 1H), 7.98 (s, 1H), 7.93 (d, J=8.4 Hz, 2H), 7.73 (d, J=8.4 Hz, 2H), 7.58 (d, J=7.6 Hz, 1H), 5.31 (t, J=6.0 Hz, 1H), 5.14-5.09 (m, 1H), 5.04-5.00 (m, 1H), 4.69-4.63 (m, 1H), 3.95 (d, J=6.4 Hz, 2H), 3.66-3.60 (m, 1H), 3.52 (q, J=7.2 Hz, 2H), 3.28-3.21 (m, 2H), 3.25 (q, J=7.2 Hz, 2H), 2.46-2.38 (m, 1H), 2.10-1.93 (m, 3H), 1.80-1.45 (m, 6H), 1.30-1.20 (m, 9H), 1.06 (d, J=6.8 Hz, 3H). Isomer SFC tR=1.741 min in 3 min chromatography (ee=91.90%).
Example 9
(R)-2-((trans-4-ethoxycyclohexyl)methyl)-N—((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)-1-isopropylisoindoline-5-carboxamide (9)




(R)-2-((trans-4-ethoxycyclohexyl)methyl)-N—((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)-1-isopropylisoindoline-5-carboxamide (9) was prepared according to General Procedure A using trans-4-ethoxycyclohexane-1-carbaldehyde and (R)—N—((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)-1-isopropylisoindoline-5-carboxamide as the starting materials. The crude residue was purified by HCl preparative HPLC Method F separation to afford the HCl salt of (R)-2-((trans-4-ethoxycyclohexyl)methyl)-N—((R)-1-(4-(ethylsulfonyl)phenyl)-2-hydroxyethyl)-1-isopropylisoindoline-5-carboxamide (9) (3.40 mg, 17%) as a white solid. LC-MS tR=0.590 min in 5-95AB_1.5 min chromatography (Welch MK RP-18e 25-2 mm), MS (ESI) m/z 557.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 7.98 (d, J=8.0 Hz, 1H), 7.95 (s, 1H), 7.89 (d, J=8.4 Hz, 2H), 7.69 (d, J=8.4 Hz, 2H), 7.55 (d, J=8.0 Hz, 1H), 5.28 (t, J=6.4 Hz, 1H), 5.13-5.07 (m, 1H), 5.03-4.97 (m, 1H), 4.64-4.60 (m, 1H), 3.92 (d, J=6.4 Hz, 2H), 3.61-3.54 (m, 3H), 3.35 (d, J=6.4 Hz, 2H), 3.23-3.19 (m, 4H), 2.45-2.35 (m, 1H), 2.23-2.00 (m, 5H), 1.97-1.80 (m, 4H), 1.26-1.17 (m, 9H), 1.03 (d, J=6.8 Hz, 3H). Isomer SFC tR=2.653 min in 3 min chromatography (ee=97.5%).
Example 10a and 10b
2-((trans-4-ethoxycyclohexyl)methyl)-N—((S)-1-(4-(ethylsulfonyl)phenyl)-3-hydroxypropyl)-1-isopropylisoindoline-5-carboxamide (10a) and (10b)




Isomer 1 (10a) was prepared according to General Procedure A using trans-4-ethoxycyclohexane-1-carbaldehyde and (S)—N—((S)-1-(4-(ethylsulfonyl)phenyl)-3-hydroxypropyl)-1-isopropylisoindoline-5-carboxamide as the starting materials. The crude residue was purified by preparative TLC (petroleum ether:ethyl acetate/1:6) and HCl preparative HPLC Method G separation to afford the HCl salt of isomer 1 (10a) (4.30 mg, 13%) as a white solid. LC-MS tR=0.601 min in 5-95 AB_1.5 min chromatography (MK RP-18e 25-2 mm), MS (ESI) m/z 571.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 9.08 (d, J=6.8 Hz, 1H), 7.96 (d, J=8.0 Hz, 1H), 7.92 (d, J=8.4 Hz, 2H), 7.90 (s, 1H), 7.70 (d, J=8.4 Hz, 2H), 7.57 (d, J=8.0 Hz, 1H), 5.41-5.39 (m, 1H), 5.08 (d, J=15.2 Hz, 1H), 4.98 (d, J=5.2 Hz, 1H), 4.88-4.86 (m, 1H), 4.63 (d, J=12.8 Hz, 1H), 3.66-3.62 (m, 2H), 3.58 (q, J=7.2 Hz, 2H), 3.27-3.26 (m, 1H), 3.25-3.21 (m, 2H), 3.22 (q, J=7.2 Hz, 2H), 2.42-2.38 (m, 1H), 2.20-2.18 (m, 4H), 2.16-2.10 (m, 1H), 1.96-1.92 (m, 2H), 1.32-1.28 (m, 2H), 1.25-1.20 (m, 11H), 1.04 (d, J=6.8 Hz, 3H). Isomer SFC tR=3.298 min in 8 min chromatography (ee=100%).
Isomer 2 (10b) was prepared in accordance with General Procedure A using trans-4-ethoxycyclohexane-1-carbaldehyde and (R)—N—((S)-1-(4-(ethylsulfonyl)phenyl)-3-hydroxypropyl)-1-isopropylisoindoline-5-carboxamide as the starting materials. The crude residue was purified by preparative TLC (petroleum ether:ethyl acetate/1:6) and HCl preparative HPLC Method G separation to afford the HCl salt of isomer 2 (10b) (4.70 mg, 12%) as a white solid. LC-MS tR=0.605 min in 5-95 AB_1.5 min chromatography (YMC-Pack ODS-AQ), MS (ESI) m/z 571.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 9.08 (d, J=8.0 Hz, 1H), 7.95 (d, J=8.0 Hz, 1H), 7.93-7.90 (m, 3H), 7.70 (d, J=8.0 Hz, 2H), 7.57 (d, J=8.0 Hz, 1H), 5.41-5.39 (m, 1H), 5.08 (d, J=15.2 Hz, 1H), 4.98 (d, J=4.8 Hz, 1H), 4.90-4.88 (m, 1H), 4.63 (d, J=15.6 Hz, 1H), 3.66-3.62 (m, 2H), 3.58 (q, J=7.2 Hz, 2H), 3.37-3.34 (m, 1H), 3.34-3.32 (m, 2H), 3.22 (q, J=7.2 Hz, 2H), 2.42-2.38 (m, 1H), 2.20-2.15 (m, 4H), 2.13-1.87 (m, 3H), 1.32-1.28 (m, 2H), 1.25-1.20 (m, 11H), 1.04 (d, J=6.8 Hz, 3H). Isomer SFC tR=3.479 min in 8 min chromatography (ee=98%).
Examples 11a, 11b, 11c and 11d
N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-2-(4-hydroxy-4-(trifluoromethyl)cyclohexyl)methyl)-1-isopropylisoindoline-5-carboxamide Isomers




To a mixture of LiAlH4 (200 mg, 5.26 mmol) in anhydrous THF (15 mL) was added a solution of 4-hydroxy-4-(trifluoromethyl)cyclohexanecarboxylic acid (500 mg, 2.36 mmol) in anhydrous THF (5 mL) at 0° C. under N2. The mixture was stirred at rt for 2 h. To the mixture was added water (0.2 mL) slowly followed by 10% NaOH solution (0.2 mL) at 0° C. The mixture was filtered and the filtrate was dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to afford crude 4-(hydroxymethyl)-1-(trifluoromethyl)cyclohexanol (480 mg, 100%) as a white solid, which was used for the next step directly without further purification. 1H NMR (CDCl3 400 MHz): δ 3.60 (d, J=6.4 Hz, 1H), 3.50 (d, J=6.4 Hz, 1H), 1.89-1.77 (m, 5H), 1.75-1.65 (m, 1H), 1.64-1.56 (m, 2H), 1.38-1.34 (m, 2H).
To a solution of (COCl)2 (0.35 mL, 3.84 mmol) in anhydrous CH2Cl2 (18 mL) was added DMSO (0.55 mL, 7.68 mmol) at −78° C. under N2. After being stirred for 30 min, a solution of 4-(hydroxymethyl)-1-(trifluoromethyl)cyclohexanol (380 mg, 1.92 mmol) in anhydrous CH2Cl2 (5 mL) was added dropwise. The mixture was stirred at −78° C. for 30 min and solution of Et3N (1.6 mL, 11.52 mmol) in anhydrous CH2Cl2 (5 mL) was added dropwise. After being stirred at −78° C. for 1 h, the mixture was warmed to rt and stirred overnight. CH2Cl2 (10 mL) was added and the organic layer was washed with water (3×20 mL) then brine (20 mL), dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The crude residue was purified by column chromatography on silica gel (eluting with 33% ethyl acetate in petroleum ether) to afford 4-hydroxy-4-(trifluoromethyl)cyclohexanecarbaldehyde (280 mg, 74%) as a yellow oil.
N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-2-(4-hydroxy-4-(trifluoromethyl)cyclohexyl)methyl)-1-isopropylisoindoline-5-carboxamide was prepared according to General Procedure A using 4-hydroxy-4-(trifluoromethyl)cyclohexanecarbaldehyde described above as the starting material to afford 4 isomers (11a to 11d). The crude product was purified by preparative TLC (eluting with 75% ethyl acetate in petroleum ether) to afford two isomers. The less polar isomeric material was separated by SFC (OJ-3) and HCl preparative HPLC Method H separation to afford HCl salts of isomer 1 (11a) (11.90 mg, 17%) and isomer 2 (11b) (10.90 mg, 16%) as white solids. The more polar isomeric material was separated by SFC (OJ-3) and HCl preparative HPLC Method H separation to afford HCl salts of isomer 3 (11c) (5.10 mg, 10%) and isomer 4 (11d) (5.00 mg, 10%) as white solids.
Isomer 1 (11a) HCl salt (11.90 mg, 17%). LC-MS tR=0.631 min in 5-95 AB_1.5 min chromatography (MK RP-18e 25-2 mm), MS (ESI) m/z 568.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 9.13 (d, J=1.6 Hz, 1H), 8.60 (dd, J=2.0, 8.4 Hz, 1H), 8.01 (d, J=8.0 Hz, 1H), 8.00 (s, 1H), 7.97 (d, J=8.0 Hz, 1H), 7.57 (d, J=8.0 Hz, 1H), 5.12 (d, J=15.2 Hz, 1H), 5.00 (d, J=4.4 Hz, 1H), 4.89 (s, 2H), 4.66 (d, J=15.2 Hz, 1H), 3.37 (q, J=7.2 Hz, 2H), 3.25-3.20 (m, 2H), 2.43-2.40 (m, 1H), 2.03-1.91 (m, 1H), 1.90-1.87 (m, 3H), 1.86-1.85 (m, 1H), 1.69-1.65 (m, 2H), 1.57-1.51 (m, 2H), 1.28 (t, J=7.2 Hz, 3H), 1.20 (d, J=6.8 Hz, 3H), 1.03 (d, J=6.8 Hz, 3H). Isomer SFC tR=4.355 min in 12 min chromatography (ee=99%).
Isomer 2 (11b) HCl salt (10.90 mg, 16%). LC-MS tR=0.636 min in 5-95 AB_1.5 min chromatography (MK RP-18e 25-2 mm), MS (ESI) m/z 568.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 8.99 (s, 1H), 8.31 (dd, J=1.6, 8.4 Hz, 1H), 7.99 (d, J=8.4 Hz, 1H), 7.96 (s, 1H), 7.70 (d, J=8.4 Hz, 1H), 7.56 (d, J=8.4 Hz, 1H), 5.10 (d, J=15.2 Hz, 1H), 4.98 (d, J=4.4 Hz, 1H), 4.81 (s, 2H), 4.64 (d, J=15.2 Hz, 1H), 3.32-3.30 (m, 1H), 3.24 (q, J=7.2 Hz, 2H), 3.22-3.20 (m, 1H), 2.42-2.39 (m, 1H), 1.98-1.97 (m, 1H), 1.88-1.85 (m, 3H), 1.70-1.69 (m, 1H), 1.68-1.66 (m, 2H), 1.55-1.51 (m, 2H), 1.24 (t, J=7.2 Hz, 3H), 1.20 (d, J=6.8 Hz, 3H), 1.03 (d, J=6.8 Hz, 3H). Isomer SFC tR=4.811 min in 12 min chromatography (ee=99%).
Isomer 3 (11c) HCl salt (5.10 mg, 10%). LC-MS tR=0.576 min in 5-95 AB_1.5 min chromatography (YMC-Pack ODS-AQ), MS (ESI) m/z 568.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 9.03 (s, 1H), 8.38 (dd, J=2.0, 8.0 Hz, 1H), 8.00 (d, J=8.4 Hz, 1H), 7.98 (s, 1H), 7.76 (d, J=8.4 Hz, 1H), 7.57 (d, J=8.0 Hz, 1H), 5.10 (d, J=15.2 Hz, 1H), 5.00 (d, J=4.0 Hz, 1H), 4.84 (s, 2H), 4.68 (d, J=15.6 Hz, 1H), 3.46-3.41 (m, 1H), 3.36-3.32 (m, 1H), 3.34 (q, J=7.2 Hz, 2H), 2.40-2.39 (m, 1H), 2.31-2.29 (m, 1H), 1.93-1.90 (m, 2H), 1.80-1.76 (m, 2H), 1.72-1.69 (m, 3H), 1.68-1.66 (m, 1H), 1.25 (t, J=7.2 Hz, 3H), 1.20 (d, J=6.8 Hz, 3H), 1.03 (d, J=6.8 Hz, 3H). Isomer SFC tR=4.528 min in 12 min chromatography (ee=99.7%).
Isomer 4 (11d) HCl salt (5.00 mg, 10%). LC-MS tR=0.573 min in 5-95 AB_1.5 min chromatography (YMC-Pack ODS-AQ), MS (ESI) m/z 568.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 9.05 (d, J=1.6 Hz, 1H), 8.43 (dd, J=2.0, 8.0 Hz, 1H), 8.00 (d, J=8.4 Hz, 1H), 7.98 (s, 1H), 7.80 (d, J=8.0 Hz, 1H), 7.57 (d, J=8.0 Hz, 1H), 5.11 (d, J=15.6 Hz, 1H), 5.01 (d, J=4.8 Hz, 1H), 4.85 (s, 2H), 4.68 (d, J=15.6 Hz, 1H), 3.46-3.41 (m, 1H), 3.36-3.32 (m, 1H), 3.32 (q, J=7.2 Hz, 2H), 2.40-2.39 (m, 1H), 2.30-2.28 (m, 1H), 2.05-2.01 (m, 2H), 1.99-1.77 (m, 2H), 1.72-1.69 (m, 3H), 1.68-1.66 (m, 1H), 1.26 (t, J=7.2 Hz, 3H), 1.20 (d, J=6.8 Hz, 3H), 1.03 (d, J=6.8 Hz, 3H). Isomer SFC tR=4.837 min in 12 min chromatography (ee=95%).
Examples 12a, 12b, 12c and 12d
N-(4-(ethylsulfonyl)benzyl)-2-((4-hydroxy-4-(trifluoromethyl)cyclohexyl)methyl)-1-isopropylisoindoline-5-carboxamide Isomers




N-(4-(ethylsulfonyl)benzyl)-2-((4-hydroxy-4-(trifluoromethyl)cyclohexyl)methyl)-1-isopropylisoindoline-5-carboxamide was prepared according to General Procedure A, using N-(4-(ethylsulfonyl)benzyl)-1-isopropylisoindoline-5-carboxamide and 4-hydroxy-4-(trifluoromethyl)cyclohexanecarbaldehyde as the starting materials to afford 4 isomers (12a to 12d). The crude product was purified by preparative TLC (eluting with 70% ethyl acetate in petroleum ether) followed by SFC then HCl preparative HPLC method H separation to afford HCl salts of isomer 1 (12a) (8.20 mg, 7%), isomer 2 (12b) (6.90 mg, 6%), isomer 3 (12c) (13.40 mg, 11%), and isomer 4 (12d) (15.80 mg, 13%) as white solids.
Isomer 1 (12a) HCl salt (8.20 mg, 7%). LC-MS tR=0.612 min in 5-95 AB_1.5 min chromatography (MK RP-18e 25-2 mm), MS (ESI) m/z 567.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 7.98 (d, J=8.8 Hz, 1H), 7.97 (s, 1H), 7.90 (d, J=8.4 Hz, 2H), 7.65 (d, J=8.4 Hz, 2H), 7.58 (d, J=8.0 Hz, 1H), 5.12 (d, J=14.8 Hz, 1H), 5.01 (d, J=5.2 Hz, 1H), 4.72 (s, 2H), 4.70 (d, J=12.4 Hz, 1H), 3.45-3.42 (m, 1H), 3.38-3.35 (m, 1H), 3.22 (q, J=7.2 Hz, 2H), 2.42-2.41 (m, 1H), 2.33-2.31 (m, 1H), 2.05-2.03 (m, 2H), 1.91-1.83 (m, 2H), 1.72-1.70 (m, 3H), 1.65-1.60 (m, 1H), 1.22 (t, J=7.2 Hz, 3H), 1.22 (d, J=6.8 Hz, 3H), 1.05 (d, J=6.8 Hz, 3H). Isomer SFC tR=8.782 min in 15 min chromatography (ee=100%).
Isomer 2 (12b) HCl salt (6.90 mg, 6%). LC-MS tR=0.607 min in 5-95 AB_1.5 min chromatography (MK RP-18e 25-2 mm), MS (ESI) m/z 567.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 7.98 (d, J=9.2 Hz, 1H), 7.97 (s, 1H), 7.90 (d, J=8.0 Hz, 2H), 7.65 (d, J=8.0 Hz, 2H), 7.58 (d, J=7.6 Hz, 1H), 5.12 (d, J=15.2 Hz, 1H), 5.01 (d, J=5.2 Hz, 1H), 4.72 (s, 2H), 4.70 (d, J=18.4 Hz, 1H), 3.47-3.42 (m, 1H), 3.38-3.37 (m, 1H), 3.21 (q, J=7.2 Hz, 2H), 2.42-2.41 (m, 1H), 2.33-2.31 (m, 1H), 2.05-2.03 (m, 2H), 1.91-1.82 (m, 2H), 1.73-1.70 (m, 3H), 1.62-1.58 (m, 1H), 1.22 (t, J=7.2 Hz, 3H), 1.22 (d, J=6.8 Hz, 3H), 1.05 (d, J=6.8 Hz, 3H). Isomer SFC tR=9.378 min in 15 min chromatography (ee=98%).
Isomer 3 (12c) HCl salt (13.40 mg, 11%). LC-MS tR=0.622 min in 5-95 AB_1.5 min chromatography (MK RP-18e 25-2 mm), MS (ESI) m/z 567.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 7.98 (d, J=9.2 Hz, 1H), 7.97 (s, 1H), 7.90 (d, J=8.0 Hz, 2H), 7.65 (d, J=8.0 Hz, 2H), 7.58 (d, J=7.6 Hz, 1H), 5.12 (d, J=14.8 Hz, 1H), 5.00 (d, J=4.4 Hz, 1H), 4.72 (s, 2H), 4.66 (d, J=15.6 Hz, 1H), 3.28-3.25 (m, 2H), 3.22 (q, J=7.2 Hz, 2H), 2.45-2.42 (m, 1H), 2.01-1.99 (m, 1H), 1.94-1.88 (m, 3H), 1.73-1.67 (m, 3H), 1.56-1.53 (m, 2H), 1.23 (d, J=7.2 Hz, 3H), 1.22 (t, J=7.2 Hz, 3H), 1.05 (d, J=6.8 Hz, 3H). Isomer SFC tR=9.717 min in 15 min chromatography (ee=96%).
Isomer 4 (12d) HCl salt (15.80 mg, 13%). LC-MS tR=0.626 min in 5-95 AB_1.5 min chromatography (MK RP-18e 25-2 mm), MS (ESI) m/z 567.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 7.98 (d, J=9.2 Hz, 1H), 7.97 (s, 1H), 7.90 (d, J=8.4 Hz, 2H), 7.65 (d, J=8.4 Hz, 2H), 7.58 (d, J=8.0 Hz, 1H), 5.12 (d, J=15.2 Hz, 1H), 5.00 (d, J=3.2 Hz, 1H), 4.72 (s, 2H), 4.66 (d, J=15.2 Hz, 1H), 3.30-3.26 (m, 2H), 3.22 (q, J=7.2 Hz, 2H), 2.43-2.42 (m, 1H), 2.06-2.05 (m, 1H), 1.94-1.88 (m, 3H), 1.71-1.68 (m, 3H), 1.57-1.53 (m, 2H), 1.23 (d, J=7.2 Hz, 3H), 1.22 (t, J=7.2 Hz, 3H), 1.05 (d, J=6.8 Hz, 3H). Isomer SFC tR=10.327 min in 15 min chromatography (ee=98%).
Examples 13a, 13b, 13c, and 13d
N-((1-(ethylsulfonyl)piperidin-4-yl)methyl)-1-isopropyl-2-((4-methoxy-4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxamide Isomers








To a solution of 4-hydroxy-4-(trifluoromethyl)cyclohexanecarboxylic acid (500 mg, 2.36 mmol) in a solution of dry THF/DMF (6 mL, v/v=1:1) was added NaH (278 mg, 6.94 mmol, 60% in mineral oil) in portions at 0° C. under N2. The mixture was stirred at 0° C. for 30 min, then MeI (2.38 g, 16.76 mmol) was added dropwise to the reaction mixture at 0° C. under N2. The mixture was stirred at 30° C. for 3.5 h. TLC analysis (eluting with petroleum ether:ethyl acetate=3:1) showed that the reaction was complete. The mixture was quenched with a sat. NH4Cl solution (40 mL) at 0° C. and extracted with ethyl acetate (3×10 mL). The combined organic layers were washed with brine (30 mL), dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to afford crude methyl 4-methoxy-4-(trifluoromethyl)cyclohexanecarboxylate (crude 560 mg, 100%) as a yellow oil, which was used for the next step directly without further purification.
To a solution of methyl 4-methoxy-4-(trifluoromethyl)cyclohexanecarboxylate (560 mg, 2.36 mmol) in anhydrous THF (10 mL) was added LiAlH4 (4.67 mL, 4.67 mmol, 1 M in THF) dropwise at 0° C. under N2. The mixture was stirred at 0° C. for 1.5 h. TLC analysis (eluting with petroleum ether:ethyl acetate=3:1) showed that the starting material was consumed completely. The mixture was quenched with water (0.18 mL) slowly followed by 10% aqueous NaOH solution (0.18 mL) at 0° C. The mixture was filtered and the filtrate was concentrated under reduced pressure to afford crude (4-methoxy-4-(trifluoromethyl)cyclohexyl)methanol (500 mg, 100%) as a colorless oil, which was used for the next step directly without further purification.
To a solution of (4-methoxy-4-(trifluoromethyl)cyclohexyl)methanol (100 mg, 0.47 mmol) in anhydrous CH2Cl2 (3 mL) was added PCC (200 mg, 0.94 mmol). The mixture was stirred at rt for 2 h. TLC analysis (eluting with petroleum ether:ethyl acetate=3:1) showed that the starting material was consumed completely. The reaction mixture was quenched with water (20 mL) and extracted with CH2Cl2 (2×15 mL). The combined organic layers were dried over anhydrous MgSO4, filtered and concentrated under reduced pressure to afford crude 4-methoxy-4-(trifluoromethyl)cyclohexanecarbaldehyde (100 mg, 100%) as a brown oil. This material was used for next step directly without further purification.
N-((1-(ethylsulfonyl)piperidin-4-yl)methyl)-1-isopropyl-2-((4-methoxy-4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxamide was prepared according to General Procedure A, using N-((1-(ethylsulfonyl)piperidin-4-yl)methyl)-1-isopropylisoindoline-5-carboxamide and 4-methoxy-4-(trifluoromethyl)cyclohexane carbaldehyde as the starting materials to afford 4 isomers (13a to 13d). The crude residue was purified by column chromatography on silica gel (eluting with a gradient of 33% to 75% EtOAc in petroleum ether) to give the crude free base. This crude free base was taken up in ethyl acetate (20 mL) and HCl/dioxane (1 mL, 4M) was added. The solution was then concentrated under reduced pressure to afford the crude product as the HCl salt (100 mg, 66%). This mixture was purified by SFC separation followed by HCl preparative HPLC method separation to afford HCl salts of isomer 1 (13a) (4.10 mg, 4%), isomer 2 (13b) (13.70 mg, 14%), and an inseparable mixture of isomers 3 and 4 (13c and 13d) (24.70 mg, 25%) as white solids.
Isomer 1 (13a) (4.10 mg, 4%) LC-MS tR=0.643 min in 5-95AB_1.5 min chromatography (Welch Sepax-Pack ODS-AQ), MS (ESI) m/z 588.2 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 8.70 (brs, 1H), 7.95-7.83 (m, 2H), 7.53 (d, J=7.2 Hz, 1H), 5.15-5.00 (m, 3H), 4.70-4.60 (m, 1H), 3.76 (d, J=12.4 Hz, 2H), 3.41 (s, 3H), 3.42-3.35 (m, 2H), 3.02 (q, J=7.6 Hz, 2H), 2.83 (t, J=12.0 Hz, 2H), 2.48-2.18 (m, 2H), 2.02-1.78 (m, 8H), 1.78-1.52 (m, 3H), 1.40-1.34 (m, 3H), 1.31 (t, J=7.6 Hz, 3H), 1.19 (d, J=6.0 Hz, 3H), 1.02 (d, J=6.0 Hz, 3H). Isomer SFC tR=7.713 min in 15 min chromatography (ee=92%).
HCl salt of isomer 2 (13b) (13.70 mg, 14%). LC-MS tR=0.639 min in 5-95AB_1.5 min chromatography (Welch Sepax-Pack ODS-AQ), MS (ESI) m/z 588.2 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 8.70 (brs, 1H), 7.95-7.83 (m, 2H), 7.54 (d, J=7.6 Hz, 1H), 5.12-5.04 (m, 1H), 5.00-4.94 (m, 2H), 4.66-4.58 (m, 1H), 3.77 (d, J=12.4 Hz, 2H), 3.40 (s, 3H), 3.36-3.32 (m, 2H), 3.27-3.15 (m, 1H), 3.02 (q, J=7.6 Hz, 2H), 2.83 (t, J=12.0 Hz, 2H), 2.45-2.32 (m, 1H), 2.14-1.92 (m, 3H), 1.90-1.72 (m, 5H), 1.70-1.57 (m, 2H), 1.50-1.30 (m, 4H), 1.31 (t, J=7.6 Hz, 3H), 1.21 (d, J=6.8 Hz, 3H), 1.02 (d, J=6.8 Hz, 3H). Isomer SFC tR=7.976 min in 15 min chromatography (ee=89%).
HCl salt mixture of isomers 3 and 4 (13c and 13d) (24.70 mg, 25%). LC-MS tR=0.637 min in 5-95AB_1.5 min chromatography (Welch MK RP-18e 25-2 mm), MS (ESI) m/z 588.2 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 8.68 (brs, 1H), 7.95-7.81 (m, 2H), 7.54 (d, J=7.2 Hz, 1H), 5.15-5.04 (m, 1H), 5.02-4.93 (m, 2H), 4.70-4.60 (m, 1H), 3.77 (d, J=12.0 Hz, 2H), 3.41 (s, 3H), 3.40-3.34 (m, 2H), 3.27-3.15 (m, 1H), 3.02 (q, J=7.2 Hz, 2H), 2.83 (t, J=12.0 Hz, 2H), 2.45-2.25 (m, 2H), 2.14-2.02 (m, 2H), 2.00-1.72 (m, 6H), 1.70-1.53 (m, 2H), 1.50-1.30 (m, 3H), 1.31 (t, J=7.2 Hz, 3H), 1.21 (d, J=5.6 Hz, 3H), 1.02 (d, J=5.6 Hz, 3H). Isomer SFC tR=8.310 and 8.680 min in 15 min chromatography (ee=36%, racemic).
Examples 14a and 14b
(S)-1-isopropyl-2-((4-methoxy-4-(trifluoromethyl)cyclohexyl)methyl)-N-((1-(methylsulfonyl)piperidin-4-yl)methyl)isoindoline-5-carboxamide Isomers




(S)-1-isopropyl-2-((4-methoxy-4-(trifluoromethyl)cyclohexyl)methyl)-N-((1-(methylsulfonyl)piperidin-4-yl)methyl)isoindoline-5-carboxamide was prepared according to General Procedure A, using (S)-1-isopropyl-N-((1-(methylsulfonyl)piperidin-4-yl)methyl)isoindoline-5-carboxamide and 4-methoxy-4-(trifluoromethyl)cyclohexane carbaldehyde as the starting materials to afford 2 isomers (14a and (14b). The crude free base was taken up in ethyl acetate (20 mL) and HCl/dioxane (1 mL, 4M) was added. The solution was then concentrated under reduced pressure to afford the crude product as the HCl salt (66 mg, 100%). This mixture was purified by SFC separation followed by HCl preparative HPLC method separation to afford isomer 1 (14a) HCl salt (4.00 mg, 12%) and isomer 2 (14b) HCl salt (4.00 mg, 12%) as white solids.
HCl salt of isomer 1 (14a) (4.00 mg, 12%). LC-MS: tR=0.776 min in 5-95AB_1.5 min chromatography (MK RP-18e 25-2 mm), MS (ESI) m/z 574.2 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 8.65 (s, 1H), 7.86 (s, 2H), 7.51 (d, J=6.8 Hz, 1H), 5.15-5.00 (m, 3H), 4.65-4.55 (m, 1H), 3.72 (d, J=12.0 Hz, 2H), 3.39 (s, 3H), 3.38-3.31 (m, 2H), 2.80 (s, 3H), 2.72 (t, J=10.8 Hz, 2H), 2.40-2.30 (m, 2H), 2.10-1.70 (m, 9H), 1.64 (q, J=14.0 Hz, 2H), 1.45-1.32 (m, 3H), 1.18 (d, J=6.4 Hz, 3H), 1.00 (d, J=6.4 Hz, 3H). Isomer SFC tR=5.415 min in 12 min chromatography (ee=100.00%).
HCl salt of isomer 2 (14b) (4.00 mg, 12%) LC-MS: tR=0.772 min in 5-95AB_1.5 min chromatography (MK RP-18e 25-2 mm), MS (ESI) m/z 574.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 8.63 (s, 1H), 7.85 (s, 2H), 7.49 (s, 1H), 5.15-5.00 (m, 3H), 4.70-4.55 (m, 1H), 3.72 (d, J=11.6 Hz, 2H), 3.39 (s, 3H), 3.35-3.30 (m, 2H), 2.80 (s, 3H), 2.72 (t, J=10.8 Hz, 2H), 2.40-2.15 (m, 2H), 2.10-1.70 (m, 8H), 1.70-1.55 (m, 3H), 1.40-1.25 (m, 3H), 1.16 (s, 3H), 0.99 (s, 3H). Isomer SFC tR=5.685 min in 12 min chromatography (ee=100.00%).
Examples 15a, 15b, 15c and 15d
N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropyl-2-((2-methyl-4,5,6,7-tetrahydrobenzo[d]oxazol-6-yl)methyl)isoindoline-5-carboxamide Isomers








To a solution of ethyl 4-oxocyclohexanecarboxylate (5.0 g, 29 mmol) in anhydrous toluene (75 mL) was added NBS (5.5 g, 31 mmol), followed by p-TsOH (450 mg, 3.0 mmol). The mixture was stirred at 110° C. for 5 h under N2. The toluene was evaporated under reduced pressure and the residue was partitioned between water (250 mL) and EtOAc (250 mL). After separation, the organic layers were washed with a saturated sodium bicarbonate solution, followed by brine (100 mL), dried over anhydrous Na2SO4 and filtered. The crude residue was purified by column chromatography on silica gel (eluting with 20% ethyl acetate in petroleum ether) to give ethyl 3-bromo-4-oxocyclohexanecarboxylate (4.0 g, 55%) as a yellow oil.
To a mixture of ethyl 3-bromo-4-oxocyclohexanecarboxylate (500 mg, 2.02 mmol) in DCE (5 mL) was added acetamide (596 mg, 10.1 mmol). The mixture was stirred at 120° C. in a sealed tube overnight. Water (10 mL) was added and the product was extracted with ethyl acetate (3×15 mL). The combined organic layers were dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by preparative TLC (eluting with 50% ethyl acetate in petroleum ether) to afford ethyl 2-methyl-4,5,6,7-tetrahydrobenzo[d]oxazole-6-carboxylate (130 mg, 31%) as a yellow solid. LC-MS tR=0.603 min in 5-95AB_1.5 min chromatography (MK RP-18e 25-2 mm, UM8505/148), MS (ESI) m/z 209.9 [M+H]+.
To a mixture of ethyl 2-methyl-4,5,6,7-tetrahydrobenzo[d]oxazole-6-carboxylate (130 mg, 0.62 mmol) in anhydrous THF (3 mL) was added LiAlH4 (71 mg, 1.86 mmol) at 0° C. The mixture was stirred at 0° C. under N2 for 3 h. 0.1 mL of H2O and 0.1 mL of a 10% aqueous NaOH solution were carefully added to the reaction mixture at 0° C. The mixture was filtered and the filtrate was concentrated under reduced pressure to afford crude (2-methyl-4,5,6,7-tetrahydrobenzo[d]oxazol-6-yl)methanol (100 mg, 96%) as a yellow oil which was used for the next step directly without further purification.
To a solution of (2-methyl-4,5,6,7-tetrahydrobenzo[d]oxazol-6-yl)methanol (100 mg, 0.60 mmol) in anhydrous DCM (5 mL) was added PCC (389 mg, 1.80 mmol) at rt. The mixture was stirred for 4 h. The reaction was quenched with water (10 mL) and extracted with ethyl acetate (3×10 mL). The combined organic layers were dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to afford crude 2-methyl-4,5,6,7-tetrahydrobenzo[d]oxazole-6-carbaldehyde (75 mg, 76%) as a yellow oil, which was used for next step directly without further purification.
N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropyl-2-((2-methyl-4,5,6,7-tetrahydrobenzo[d]oxazol-6-yl)methyl)isoindoline-5-carboxamide was prepared according to General Procedure A, using 2-methyl-4,5,6,7-tetrahydrobenzo[d]oxazole-6-carbaldehyde as the starting material to afford 4 isomers (15a-15d). The crude residue was purified by preparative TLC (eluting with 50% ethyl acetate in petroleum ether) followed by SFC (AD-H) then HCl preparative HPLC to afford isomer 1 (15a) HCl salt (5.90 mg, 7%), isomer 2 (15b) HCl salt (8.40 mg, 9%), isomer 3 (15c) HCl salt (9.60 mg, 11%) and isomer 4 (15d) HCl salt (4.00 mg, 4%) as white solids.
HCl salt of isomer 1 (15a) (5.90 mg, HCl salt, 7%). LC-MS tR=0.596 min in 5-95AB_1.5 min chromatography (MK RP-18e 25-2 mm, μm 8505/148), MS (ESI) m/z 537.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 9.06 (d, J=2.0 Hz, 1H), 8.45 (dd, J=2.4, 8.4 Hz, 1H), 8.03-8.01 (m, 2H), 7.83 (d, J=8.4 Hz, 1H), 7.59 (d, J=8.4 Hz, 1H), 5.19 (d, J=14.8 Hz, 1H), 5.07 (d, J=4.0 Hz, 1H), 4.87 (s, 2H), 4.77-4.73 (m, 1H), 3.55-3.45 (m, 2H), 3.31 (q, J=6.8 Hz, 2H), 3.20-3.10 (m, 1H), 2.80-2.65 (m, 7H), 2.55-2.45 (m, 1H), 2.35-2.30 (m, 1H), 1.83-1.80 (m, 1H), 1.29-1.22 (m, 6H), 1.06 (d, J=6.8 Hz, 3H). Isomer SFC tR=4.130 min in 10 min chromatography (ee=94%).
HCl salt of isomer 2 (15b) (8.40 mg, HCl salt, 9%). LC-MS tR=0.599 min in 5-95AB_1.5 min chromatography (MK RP-18e 25-2 mm, UM8505/148), MS (ESI) m/z 537.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 9.06 (d, J=2.0 Hz, 1H), 8.46 (dd, J=2.4, 8.4 Hz, 1H), 8.03-8.01 (m, 2H), 7.85 (d, J=8.4 Hz, 1H), 7.59 (d, J=8.4 Hz, 1H), 5.20 (d, J=15.6 Hz, 1H), 5.09 (d, J=4.0 Hz, 1H), 4.87 (s, 2H), 4.72 (d, J=15.2 Hz, 1H), 3.51 (d, J=4.8 Hz, 1H), 3.32 (q, J=7.2 Hz, 2H), 3.20-3.10 (m, 1H), 2.79 (s, 3H), 2.75-2.60 (m, 4H), 2.55-2.45 (m, 1H), 2.30-2.20 (m, 1H), 1.80-1.70 (m, 1H), 1.29-1.24 (m, 6H), 1.07 (d, J=6.4 Hz, 3H). Isomer SFC tR=4.872 min in 10 min chromatography (ee=98%).
HCl salt of isomer 3 (15c) (9.60 mg, HCl salt, 11%). LC-MS tR=0.593 min in 5-95AB_1.5 min chromatography (MK RP-18e 25-2 mm, UM8505/148), MS (ESI) m/z 537.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 9.07 (d, J=2.0 Hz, 1H), 8.49 (dd, J=2.0, 8.4 Hz, 1H), 8.03-8.01 (m, 2H), 7.74 (d, J=8.4 Hz, 1H), 7.59 (d, J=8.8 Hz, 1H), 5.20 (d, J=14.8 Hz, 1H), 5.10 (d, J=4.0 Hz, 1H), 4.88 (s, 2H), 4.72 (d, J=15.2 Hz, 1H), 3.51 (d, J=4.8 Hz, 1H), 3.31 (q, J=7.6 Hz, 2H), 3.20-3.10 (m, 1H), 2.80 (s, 3H), 2.75-2.65 (m, 4H), 2.55-2.45 (m, 1H), 2.30-2.25 (m, 1H), 1.80-1.70 (m, 1H), 1.29-1.24 (m, 6H), 1.07 (d, J=6.4 Hz, 3H). Isomer SFC tR=7.005 min in 10 min chromatography (ee=100%).
HCl salt of isomer 4 (15d) (4.00 mg, 4%). LC-MS tR=0.598 min in 5-95AB_1.5 min chromatography (MK RP-18e 25-2 mm, UM8505/148), MS (ESI) m/z 537.0 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 9.02 (d, J=2.0 Hz, 1H), 8.35 (dd, J=2.0 and 8.0 Hz, 1H), 8.02-8.00 (m, 2H), 7.74 (d, J=8.4 Hz, 1H), 7.59 (d, J=8.0 Hz, 1H), 5.20 (d, J=14.8 Hz, 1H), 5.06-5.05 (m, 1H), 4.84 (s, 2H), 4.76-4.70 (m, 1H), 3.55-3.45 (m, 2H), 3.32 (q, J=7.2 Hz, 2H), 3.20-3.10 (m, 1H), 2.71 (s, 3H), 2.73-2.60 (m, 4H), 2.55-2.45 (m, 1H), 2.30-2.25 (m, 1H), 1.85-1.75 (m, 1H), 1.29-1.23 (m, 6H), 1.06 (d, J=6.4 Hz, 3H). Isomer SFC tR=5.149 min in 10 min chromatography (ee=100%).
Examples 16a and 16b
(S)—N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropyl-2-((-6-(trifluoromethyl)tetrahydro-2H-pyran-3-yl)methyl)isoindoline-5-carboxamide Isomers








To a solution of 1,1,1-trifluoropent-4-en-2-ol (6.7 g, 48 mmol) in anhydrous DMF (85 mL) (dried with CaH2) was added NaH (2.3 g, 57 mmol, 60% in mineral oil) in portions at 0° C. The mixture was stirred at 0° C. for 30 min and ethyl 2-(bromomethyl)acrylate (9.2 g, 48 mmol) was added dropwise to the resulting mixture via syringe at 0° C. After addition, the mixture was stirred at rt for 2 h. TLC analysis (eluting with petroleum ether:ethyl acetate=10:1) showed that the starting material was consumed. The reaction was quenched with water (50 mL) at 0° C. and the aqueous layer was extracted with ethyl acetate (3×50 mL). The combined organic layers were washed successively with water (3×50 mL), brine (50 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude residue was purified by column chromatography on silica gel (eluting with petroleum ether/ethyl acetate:gradient from 100/1 to 50/1) to afford ethyl 2-(((1,1,1-trifluoropent-4-en-2-yl)oxy)methyl)acrylate (6.6 g, 55%) as a pale yellow oil. 1H NMR (CDCl3 400 MHz): δ 6.31 (s, 1H), 5.89 (s, 1H), 5.85-5.74 (m, 1H), 5.23-5.07 (m, 2H), 4.52-4.43 (m, 1H), 4.38-4.15 (m, 3H), 3.82-3.68 (m, 1H), 2.50-2.35 (m, 2H), 1.38-1.20 (m, 3H).
To a solution of ethyl 2-(((1,1,1-trifluoropent-4-en-2-yl)oxy)methyl)acrylate (6.6 g, 26.2 mmol) in anhydrous CH2Cl2 (2.6 L) was added Grubbs II catalyst (2.2 g, 2.62 mmol) under N2. The mixture was stirred at rt for 3 h. TLC analysis (eluting with petroleum ether:ethyl acetate=10:1) showed that the reaction was complete. Water (2 L) was added to the mixture to quench the reaction. After partition, the organic layer was washed successively with water (3×2 L) then brine (2 L), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica gel (eluting with petroleum ether/ethyl acetate: gradient from 100/1 to 80/1) to afford ethyl 6-(trifluoromethyl)-5,6-dihydro-2H-pyran-3-carboxylate (4.83 g, 82%) as a pale yellow oil. 1H NMR (CDCl3 400 MHz): δ 7.01 (d, J=2.8 Hz, 1H), 4.63-4.58 (m, 1H), 4.40-4.33 (m, 1H), 4.20 (q, J=7.2 Hz, 2H), 3.95-3.84 (m, 1H), 2.57-2.46 (m, 1H), 2.41-2.32 (m, 1H), 1.28 (t, J=7.2 Hz, 3H).
To a solution of ethyl 6-(trifluoromethyl)-5,6-dihydro-2H-pyran-3-carboxylate (4.83 g, 22 mmol) in anhydrous THF (130 mL) was added dry Pd(OH)2 on carbon (2.7 g, 10% w/w). The mixture was stirred at rt for 16 h under H2 (30 psi). TLC analysis (eluting with petroleum ether/ethyl acetate=10/1) showed that most of the starting material was not consumed. The mixture was filtered, then the filtrate was concentrated under reduced pressure and dissolved into anhydrous THF (60 mL). Dry Pd(OH)2 on carbon (2.7 g, 10% w/w) was added to the mixture. The mixture was stirred at rt for 28 h under H2 (30 psi). TLC analysis (eluting with petroleum ether/ethyl acetate=10/1) showed that the starting material was consumed. The mixture was filtered and the filtrate was concentrated under reduced pressure to afford crude ethyl 6-(trifluoromethyl)tetrahydro-2H-pyran-3-carboxylate (3.4 g, 70%) as a colorless oil, which was used for the next step directly without further purification. 1H NMR (CDCl3 400 MHz): δ 4.50 (d, J=11.6 Hz, 1H), 4.18 (q, J=7.2 Hz, 2H), 3.80-3.68 (m, 1H), 3.66 (d, J=3.2, 11.6 Hz, 1H), 2.55-2.49 (m, 1H), 2.43-2.35 (m, 1H), 1.95-1.81 (m, 1H), 1.75-1.65 (m, 2H), 1.25 (t, J=7.2 Hz, 3H).
To a solution of crude ethyl 6-(trifluoromethyl)tetrahydro-2H-pyran-3-carboxylate (2.0 g, 8.8 mmol) in THF (24 mL), EtOH (12 mL) was added 1 N aqueous NaOH solution (12 mL). The mixture was stirred at rt for 3 h. TLC analysis (eluting with petroleum ether:ethyl acetate=10:1) showed that the reaction was complete. The mixture was added to water (20 mL) and concentrated under reduced pressure to remove the organic solvent. The residue was washed with MTBE (20 mL) and adjusted to pH=4-5 with 1 N HCl solution. Then, the aqueous layer was extracted with EtOAc (3×20 mL). The combined organic layers were washed successively with brine (20 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to afford crude 6-(trifluoromethyl)tetrahydro-2H-pyran-3-carboxylic acid (1.72 g, 98%) as a pale yellow oil, which was used for the next step directly without further purification. The ratio of cis:trans isomers was  ̃2:1 based on 1H NMR and 19F NMR analysis.
To a solution of crude 6-(trifluoromethyl)tetrahydro-2H-pyran-3-carboxylic acid (1.72 g, 8.69 mmol) was added a 2 N aqueous NaOH solution (76 mL). The mixture was stirred in sealed tube at 100° C. for 84 h. The mixture was added to water (20 mL) and washed with MTBE (50 mL). The aqueous layer was adjusted to pH=4-5 with 1 N HCl solution and extracted with EtOAc (3×50 mL). The combined organic layers were washed successively with brine (50 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to afford crude 6-(trifluoromethyl)tetrahydro-2H-pyran-3-carboxylic acid (1.60 g, 93%) as a pale yellow oil, which was used for the next step directly without further purification. The ratio of cis:trans was 1:3 based on 1H NMR and 19F NMR analysis.
To a solution of crude 6-(trifluoromethyl)tetrahydro-2H-pyran-3-carboxylic acid (1.0 g, 5.01 mmol) ( ̃1:3 cis:trans ratio of isomers) in anhydrous CH2Cl2 (60 mL) was added N,O-dimethylhydroxylamine hydrochloride (980 mg, 10.10 mmol), EDCI (1.93 g, 10.10 mmol), HOBt (1.36 g, 10.10 mmol), and DIPEA (1.95 g, 15.15 mmol). The mixture was stirred at rt for 16 h. The mixture was added to water (60 mL) and extracted with CH2Cl2 (3×60 mL). The combined organic layers were washed successively with brine (60 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica gel (eluting with petroleum ether/ethyl acetate: gradient from 30/1 to 15/1) to afford N-methoxy-N-methyl-6-(trifluoromethyl)tetrahydro-2H-pyran-3-carboxamide (1.05 g, 87%) as a pale yellow oil. The ratio of cis:trans was 1:3 based on 1H NMR and 19F NMR analysis. 1H NMR (CDCl3 400 MHz): δ 4.30-4.24 (m, 0.25H), 4.22-4.15 (m, 0.75H), 3.90-3.68 (m, 4H), 3.62-3.52 (m, 1H), 3.24-3.14 (m, 2H), 3.10-2.98 (m, 1H), 2.14-2.04 (m, 1H), 1.95-1.80 (m, 2H), 1.80-1.65 (m, 2H).
To a solution of N-methoxy-N-methyl-6-(trifluoromethyl)tetrahydro-2H-pyran-3-carboxamide (90 mg, 0.373 mmol) ( ̃1:3 cis:trans ratio of isomers) in anhydrous THF (5 mL) was added LiAlH4 (0.75 mL, 0.746 mmol, 1 M in THF) dropwise at 0° C. under N2. The mixture was stirred at 0° C. for 1 h. TLC analysis (eluting with petroleum ether/ethyl acetate: 5/1) showed that the reaction was complete. The mixture was quenched with a sat. Na2SO4 solution (1 mL) and filtered. The filtrate was diluted with DCM (60 mL) and washed with water (60 mL), a 10% HCl solution (0.5 M, 60 mL), a sat. NaHCO3 solution (60 mL) and then water (60 mL), respectively. The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to afford crude 6-(trifluoromethyl)tetrahydro-2H-pyran-3-carbaldehyde (60 mg, 88%) as a pale yellow oil, which was used for the next step directly without further purification. The ratio of cis:trans was 1:3 based on 1H NMR and 19F NMR analysis.
(S)—N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropyl-2-((-6-(trifluoromethyl)tetrahydro-2H-pyran-3-yl)methyl)isoindoline-5-carboxamide was prepared according to General Procedure A using (S)—N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropylisoindoline-5-carboxamide and 6-(trifluoromethyl)tetrahydro-2H-pyran-3-carbaldehyde ( ̃1:3 ratio of cis:trans isomers) as the starting materials. The crude material was separated by SFC (IC-3) to give two crude isomer products (16a and 16b), which were purified by HCl preparative HPLC separation, then lyophilized directly to afford isomer 1 (16a) HCl salt (30.10 mg, 23%) and isomer 2 (16b) HCl salt (17.80 mg, 14%) as white solids.
HCl salt of isomer 1 (16a) (30.10 mg, 23%). LC-MS tR=0.642 min in 5-95AB_1.5 min chromatography (Welch MK RP-18e 25-2 mm), MS (ESI) m/z 554.1 [M+H]+. Isomer SFC tR=6.705 min in 15 min chromatography (ee=100.00%). 1H NMR (CD3OD 400 MHz): δ 9.01 (s, 1H), 8.34 (d, J=8.0 Hz, 1H), 7.99 (d, J=8.4 Hz, 1H), 7.97 (s, 1H), 7.73 (d, J=8.0 Hz, 1H), 7.57 (d, J=8.4 Hz, 1H), 5.10 (d, J=15.2 Hz, 1H), 5.00 (d, J=4.4 Hz, 1H), 4.96-4.90 (m, 1H), 4.82 (s, 2H), 4.62 (d, J=15.2 Hz, 1H), 4.28 (d, J=10.8 Hz, 1H), 3.93-3.83 (m, 1H), 3.34 (q, J=7.2 Hz, 2H), 3.21 (d, J=6.0 Hz, 2H), 2.50-2.38 (m, 1H), 2.33-2.10 (m, 2H), 1.95-1.87 (m, 1H), 1.74-1.63 (m, 1H), 1.50-1.37 (m, 1H), 1.25 (t, J=7.2 Hz, 3H), 1.20 (d, J=6.8 Hz, 3H), 1.03 (d, J=6.8 Hz, 3H).
Isomer 2 (16b) (17.80 mg, 14%) LC-MS tR=0.639 min in 5-95AB_1.5 min chromatography (Welch MK RP-18e 25-2 mm), MS (ESI) m/z 554.0 [M+H]+. Isomer SFC tR=8.160 min in 15 min chromatography (ee=100.00%). 1H NMR (CD3OD 400 MHz): δ 9.00 (s, 1H), 8.33 (d, J=8.0 Hz, 1H), 7.99 (d, J=8.4 Hz, 1H), 7.97 (s, 1H), 7.72 (d, J=8.4 Hz, 1H), 7.56 (d, J=8.0 Hz, 1H), 5.10 (d, J=15.2 Hz, 1H), 5.03-4.93 (m, 2H), 4.82 (s, 2H), 4.65 (d, J=15.2 Hz, 1H), 4.19 (d, J=10.8 Hz, 1H), 3.93-3.83 (m, 1H), 3.40-3.32 (m, 2H), 3.25-3.13 (m, 2H), 2.50-2.38 (m, 1H), 2.33-2.16 (m, 2H), 1.96-1.87 (m, 1H), 1.74-1.60 (m, 1H), 1.56-1.47 (m, 1H), 1.25 (t, J=7.2 Hz, 3H), 1.20 (d, J=6.8 Hz, 3H), 1.01 (d, J=6.8 Hz, 3H).
Example 17
(S)-1-ethyl-N—((R)-1-(5-(ethylsulfonyl)pyridin-2-yl)-2-hydroxyethyl)-2-((trans-4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxamide (17)
General Procedure B: (S)-1-ethyl-N—((R)-1-(5-(ethylsulfonyl)pyridin-2-yl)-2-hydroxyethyl)-2-((trans-4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxamide




A mixture of (S)-1-ethyl-N—((R)-1-(5-(ethylsulfonyl)pyridin-2-yl)-2-hydroxyethyl)isoindoline-5-carboxamide (40 mg, 0.1 mmol), trans-4-(trifluoromethyl)cyclohexanecarbaldehyde (27 mg, 0.15 mmol), NaBH3CN (24 mg, 0.4 mmol) and HOAc (50 μL) (pH=6-7) in MeOH (4 mL) was stirred at 70° C. for 1 h. LCMS showed no starting material remained. The mixture was concentrated under reduced pressure. The residue was basified to pH=8 with saturated NaHCO3 solution and extracted with ethyl acetate (3×30 mL). The combined organic layers were dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The crude residue was purified by preparative TLC (ethyl acetate) to give the product (20 mg, 36%) as an oil, which was purified by SFC separation (AD-H) followed by acidic (HCl) preparative HPLC to afford (S)-1-ethyl-N—((R)-1-(5-(ethylsulfonyl)pyridin-2-yl)-2-hydroxyethyl)-2-((trans-4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxamide (17) HCl salt (2.3 mg, 12%) as a white solid. LC-MS tR=0.623 min in 5-95AB_1.5 min chromatography (MK RP-18e 25-2 mm), MS (ESI) m/z 568.2 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 9.04 (d, J=1.6 Hz, 1H), 8.31 (dd, J=8.0, 2.0 Hz, 1H), 8.03 (d, J=8.0 Hz, 1H), 7.97 (s, 1H), 7.76 (d, J=8.0 Hz, 1H), 7.59 (d, J=8.0 Hz, 1H), 5.39 (t, J=6.8 Hz, 1H), 4.92-4.93 (m, 1H), 4.68-4.85 (m, 2H), 4.06-4.08 (m, 2H), 3.33-3.34 (m, 2H), 3.23 (q, J=7.2 Hz, 2H), 2.00-2.22 (m, 8H), 1.46-1.47 (m, 2H), 1.07-1.30 (m, 8H). 19F NMR (CD3OD 400 MHz): δ −75.41. Isomer SFC tR=2.307 min in 3 min chromatography (Column: AD-H_3 UM_4_5_40_4ML), ee=100%.
The following compounds in Table 2 were prepared using General Procedure B with trans-4-(trifluoromethyl)cyclohexane-1-carbaldehyde and intermediates derived from 2-(tert-butoxycarbonyl)-1-ethylisoindoline-5-carboxylic acid, and the appropriate intermediates described herein.










TABLE 2





Ex.





No.
Structure
LCMS
1H-NMR







18




568.2 [M + H]+
(CD3OD 400 MHz): δ 9.04 (d, J = 2.0 Hz, 1H), 8.30 (dd, J = 8.0, 2.0 Hz, 1H), 8.03 (d, J = 7.6 Hz, 1H), 7.96 (s, 1H), 7.75 (d, J = 8.4 Hz, 1H), 7.59 (d, J = 8.0 Hz, 1H), 5.40 (t, J = 6.8 Hz, 1H), 4.92- 4.93 (m, 1H), 4.79-4.87 (m, 2H), 4.05- 4.07 (m, 2H), 3.33-3.34 (m, 2H), 3.23 (q, J = 7.2 Hz, 2H), 2.04-2.22 (m, 8H), 1.46-1.47 (m, 2H), 1.24-1.31 (m, 8H)


 


19a




567.1 [M + H]+
(CD3OD 400 MHz): δ 7.97-8.01 (m, 2H), 7.91 (d, J = 8.4 Hz, 2H), 7.72 (d, J = 8.4 Hz, 2H), 7.56 (d, J = 8.4 Hz, 1H), 5.29 (t, J = 6.0 Hz, 1H), 5.08 (d, J = 14.8 Hz, 1H), 4.91-4.97 (m, 1H), 4.65 (d, J = 14.8 Hz, 1H), 3.94 (d, J = 6.4 Hz, 2H), 3.34-3.43 (m, 1H), 3.26-3.32 (m, 1H), 3.22 (dd, J = 14.8, 7.6 Hz, 2H), 1.85-2.32 (m, 8H), 1.37-1.57 (m, 2H), 1.01-1.30 (m, 8H)


 


19b




567.1 [M + H]+
(CD3OD 400 MHz): δ 7.90-7.97 (m, 4H), 7.71 (d, J = 8.0 Hz, 2H), 7.52 (d, J = 6.8 Hz, 1H), 5.29 (t, J = 6.4 Hz, 1H), 4.31-4.85 (m, 3H), 3.93 (d, J = 6.4 Hz, 2H), 3.22 (dd, J = 14.8, 7.6 Hz, 3H), 1.77-2.26 (m, 9H), 1.35-1.52 (m, 2H), 1.05-1.25 (m, 8H)


 


20a




582.2 [M + H]+
(CD3OD 400 MHz): δ 8.99 (d, J = 2.0 Hz, 1H), 8.27 (d, J = 8.8 Hz, 1H), 7.95 (d, J = 8.0 Hz, 1H), 7.90 (s, 1H), 7.72 (d, J = 8.0 Hz, 1H), 7.53-7.55 (d, J = 8.0 Hz, 1H), 5.42-5.46 (m, 1H), 5.01- 5.05 (m, 2H), 4.59-4.63 (m, 1H), 3.65- 3.70 (m, 2H), 3.32-3.34 (m, 1H), 3.28- 3.29 (m, 1H), 2.12-2.26 (m, 11H), 2.00- 2.03 (m, 2H), 1.19-1.25 (m, 9H)


 


20b




582.2 [M + H]+
(CD3OD 400 MHz): δ 9.03 (d, J = 2.0 Hz, 1H), 8.36 (d, J = 8.0 Hz, 1H), 7.92- 7.97 (m, 2H), 7.81 (d, J = 8.4 Hz, 1H), 7.55 (d, J = 8.4 Hz, 1H), 5.44-5.47 (m, 1H), 5.02-5.08 (m, 2H), 4.61-4.67 (m, 1H), 3.66-3.74 (m, 2H), 3.38-3.47 (m, 1H), 3.28-3.29 (m, 1H), 2.16-2.25 (m, 12H), 2.01-2.04 (m, 2H), 1.17-1.27 (m, 8H)


 


21a




538.1 (M + H)+
(CD3OD 400 MHz): δ 9.00 (d, J = 2.0 Hz, 1H), 8.30 (dd, J = 8.4, 2.0 Hz, 1H), 7.93-8.04 (m, 2H), 7.69 (d, J = 8.4 Hz, 1H), 7.60 (d, J = 8.0 Hz, 1H), 5.08 (d, J = 14.0 Hz, 1H), 4.81-4.83 (m, 2H), 4.66 (d, J = 14.0 Hz, 1H), 3.27-3.31 (m, 2H), 2.15-2.28 (m, 6H), 1.99-2.13 (m, 4H), 1.39-1.54 (m, 2H), 1.32 (s, 2H), 1.18- 1.30 (m, 7H).


 


21b




538.1 (M + H)+
(CD3OD 400 MHz): δ 8.98 (d, J = 2.0 Hz, 1H), 8.36 (dd, J = 8.4, 2.0 Hz, 1H), 7.94-8.04 (m, 2H), 7.77 (d, J = 8.4 Hz, 1H), 7.59 (d, J = 8.0 Hz, 1H), 5.10 (d, J = 15.2 Hz, 1H), 4.84 (s, 2H), 4.67 (d, J = 14.8 Hz, 1H), 3.28-3.32 (m, 2H), 2.11-2.31 (m, 5H), 1.92-2.11 (m, 5H), 1.39-1.53 (m, 2H), 1.10-1.34 (m, 9H).


 


22




538.2 (M + H)+
(CD3OD 400 MHz): δ 7.90-8.00 (m, 2H), 7.79-7.85 (m, 2H), 7.52-7.61 (m, 3H), 4.96-5.14 (m, 2H), 4.69 (s, 3H), 3.34-3.41 (m, 1H), 3.23-3.31 (m, 1H), 2.53 (s, 3H), 1.82-2.27 (m, 8H), 1.37- 1.53 (m, 2H), 1.06-1.32 (m, 5H).


 


23a




539.0 [M + H]+
(CD3OD 400 MHz): δ 8.98 (d, J = 2.0 Hz, 1H), 8.35 (dd, J = 8.0, 2.0 Hz, 1H), 7.99-8.03 (m, 2H), 7.77 (d, J = 8.4 Hz, 1H), 7.59 (d, J = 8.4 Hz, 1H), 5.09 (d, J = 15.2 Hz, 1H), 4.92-4.94 (m, 1H), 4.85-4.89 (m, 3H), 4.67 (d, J = 14.8 Hz, 1H), 3.36-3.39 (m, 1H), 2.61 (s, 3H), 2.01-2.23 (m, 8H), 1.45-1.48 (m, 2H), 1.07-1.27 (m, 5H)


 


23b




539.0 [M + H]+
(CD3OD 400 MHz): δ 8.98 (d, J = 1.6 Hz, 1H), 8.32 (dd, J = 8.4, 2.4 Hz, 1H), 8.03-7.98 (m, 2H), 7.75 (d, J = 8.4 Hz, 1H), 7.59 (d, J = 8.0 Hz, 1H), 5.09 (d, J = 14.8 Hz, 1H), 4.92-4.94 (m, 1H), 4.86-4.89 (m, 3H), 4.67 (d, J = 15.2 Hz, 1H), 3.39-3.42 (m, 1H), 2.60 (s, 3H), 2.01-2.24 (m, 8H), 1.45-1.48 (m, 2H), 1.05-1.27 (m, 5H).









Example 24a
(R)-2-(-1-ethyl-2-((trans-4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxamido)-2-(4-(ethylsulfonyl)phenyl)ethyl carbamate (24a and 24b)




To a solution of (19a) (20 mg, 0.033 mmol) in anhydrous CH2Cl2 (2 mL) was added 2,2,2-trichloroacetyl isocyanate (31 mg, 0.165 mmol) at 0° C. under a nitrogen atmosphere. Then the reaction was stirred at 24-31° C. for 1 h. The reaction mixture was checked by LCMS, which showed that the starting material was consumed. The reaction was quenched with MeOH (5 mL) and the solvents were removed under reduced pressure to afford the crude residue which was redissolved in MeOH (2 mL). Solid K2CO3 (14 mg, 0.099 mmol) was then added and the reaction mixture stirred at 24-31° C. for 1 h. The solvents were removed under reduced pressure to afford the crude residue which was purified by preparative HPLC (HCl) to give isomer 1 (24a) (15.00 mg, 73%) as a white solid. LC-MS tR=0.819 min in 10-80AB_2.0 min chromatography (Welch Xtimate C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 610.2 [M+H]+. 1H NMR (CD3OD 400 MH): δ 7.92-7.99 (m, 4H), 7.74 (d, J=8.4 Hz, 2H), 7.57 (d, J=8.0 Hz, 1H), 5.47-5.55 (m, 1H), 4.94-5.13 (m, 2H), 4.60-4.71 (m, 1H), 4.43 (d, J=6.8 Hz, 2H), 3.33-3.42 (m, 1.5H), 3.27-3.31 (m, 0.5H), 3.22 (dd, J=14.8, 7.6 Hz, 2H), 1.90-2.29 (m, 8H), 1.38-1.54 (m, 2H), 0.96-1.32 (m, 8H). 19F NMR (CD3OD 400 MHz): δ −75.35. Isomer SFC tR=11.935 min in 18.0 min chromatography (AD-H_5_5_40_2, 35 mL, ee=96.97%).
Isomer 2 (24b) was prepared in accordance with Scheme 24 using (19b) as the starting material. LC-MS tR=0.827 min in 10-80AB_2.0 min chromatography (Welch Xtimate C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 610.2 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 7.92-7.99 (m, 4H), 7.74 (d, J=8.4 Hz, 2H), 7.57 (d, J=8.4 Hz, 1H), 5.51 (t, J=6.4 Hz, 1H), 4.92-5.14 (m, 2H), 4.57-4.75 (m, 1H), 4.43 (d, J=6.8 Hz, 2H), 3.34-3.54 (m, 1.5H), 3.27-3.32 (m, 0.5H), 3.22 (dd, J=14.8, 7.6 Hz, 2H), 1.64-2.37 (m, 9H), 1.36-1.55 (m, 2H), 1.15-1.30 (m, 7H). 19F NMR (CD3OD 400 MHz): δ −75.35. Isomer SFC tR=11.104 min in 18.0 min chromatography (AD-H_5_5_40_2, 35 mL, ee=64.23%).
Examples 25a and 25b
1-ethyl-N—((S)-1-(4-(ethylsulfonyl)phenyl)-3-hydroxypropyl)-2-((trans-4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxamide (25a and 25b)




To a solution of (S)-2-(tert-butoxycarbonyl)-1-ethylisoindoline-5-carboxylic acid (150 mg, 0.50 mmol) and (S)-methyl 3-amino-3-(4-(ethylsulfonyl)phenyl)propanoate (136 mg, 0.50 mmol) in DMF (5 mL) was added HATU (286 mg, 0.75 mmol) and DIEA (0.4 mL, 2.50 mmol, d=0.782 g/mL) under a nitrogen atmosphere. After addition, the reaction mixture was stirred at 27 ̃30° C. for 2 h. The reaction mixture was checked by LCMS, which showed that the starting material was consumed. The reaction mixture was diluted with ethyl acetate (40 mL), washed with brine (3×30 mL), dried over anhydrous Na2SO4, filtered and concentrated by rotary evaporation. The crude residue was purified by column chromatography on silica gel (eluting with petroleum ether:ethyl acetate=gradient from 2:1 to 1:2) to afford (S)-tert-butyl 1-ethyl-5-(((S)-1-(4-(ethylsulfonyl)phenyl)-3-methoxy-3-oxopropyl)carbamoyl)isoindoline-2-carboxylate (100 mg, 71%) as a brown solid. LC-MS tR=1.155 min in 10-80AB_2.0 min chromatography (Welch Xtimate C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 545.3 [M+H]+.
To a solution of (S)-tert-butyl 1-ethyl-5-(((S)-1-(4-(ethylsulfonyl)phenyl)-3-methoxy-3-oxopropyl)carbamoyl)isoindoline-2-carboxylate (200 mg, 0.36 mmol) in anhydrous dichloromethane (4.0 mL) was added an HCl in dioxane solution (1.0 mL, 4.0 mmol, 4N). The reaction mixture was stirred at 27 ̃34° C. for 16 h. TLC analysis (eluting with petroleum ether:ethyl acetate=1:2) showed that the starting material was consumed. The reaction was cooled down with an ice-water bath for 5 min, basified to pH=12 ̃14 with 1 N NaOH solution, diluted with water (10 mL) and extracted with ethyl acetate (3×20 mL). The combined organics were washed with brine (3×50 mL), dried over anhydrous Na2SO4, filtered and concentrated by rotary evaporation to afford (S)-methyl 3-((S)-1-ethylisoindoline-5-carboxamido)-3-(4-(ethylsulfonyl)phenyl)propanoate (100 mg, 61%) as a brown oil. This material was used directly for the next step without further purification.
To a solution of (S)-methyl 3-((S)-1-ethylisoindoline-5-carboxamido)-3-(4-(ethylsulfonyl)phenyl)propanoate (100 mg, 0.22 mmol) in anhydrous MeOH (5 mL) and HOAc (0.05 mL) was added trans-4-(trifluoromethyl)cyclohexanecarbaldehyde (60 mg, 0.33 mmol) and NaBH3CN (28 mg, 0.44 mmol) under a nitrogen atmosphere. After addition, the reaction mixture was heated to 40° C. for 1 h. LCMS analysis showed that the starting material was consumed. After cooling to rt, the reaction basified to pH=10-11 with 1N NaOH solution, diluted with water (20 mL) and extracted with ethyl acetate (3×20 mL). The combined organics were washed with brine (3×40 mL), dried over anhydrous Na2SO4, filtered and concentrated by rotary evaporation. The crude residue was purified by preparative TLC (eluting with petroleum ether:ethyl acetate=1:1) to give (S)-methyl 3-((S)-1-ethyl-2-((trans-4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxamido)-3-(4-(ethylsulfonyl)phenyl)propanoate (100 mg, 68%) as a brown solid. LC-MS tR=0.946 min in 10-80AB_2.0 min chromatography (Welch Xtimate C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 609.3 [M+H]+.
To a solution of (S)-methyl 3-((S)-1-ethyl-2-((trans-4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxamido)-3-(4-(ethylsulfonyl)phenyl)propanoate (100 mg, 0.15 mmol) in anhydrous MeOH (5 mL) was added NaBH4 (114 mg, 3.00 mmol) under a nitrogen atmosphere. After addition, the reaction mixture was stirred at 27 ̃34° C. for 16 h. The reaction mixture was checked by LCMS, which showed that the starting material was nearly consumed. The reaction was quenched with water (20 mL) and extracted with ethyl acetate (3×20 mL). The combined organics were washed with brine (3×50 mL), dried over anhydrous Na2SO4, filtered and concentrated by rotary evaporation to afford the crude residue which was purified by preparative TLC (petroleum ether:ethyl acetate=1:2) to give (S)-1-ethyl-N—((S)-1-(4-(ethylsulfonyl)phenyl)-3-hydroxypropyl)-2-((trans-4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxamide (12 mg, 13%) as a brown solid.
This product was then purified by SFC (AD-H) followed preparative HPLC (HCl) to give the HCl salt of isomer 1 (25a) (4.40 mg, 37%) as a white solid. LC-MS tR=0.918 min in 10-80AB_2.0 min chromatography (Welch Xtimate C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 581.3 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 9.08 (d, J=7.6 Hz, 1H), 7.96 (d, J=8.4 Hz, 1H), 7.89-7.93 (m, 3H), 7.68-7.72 (m, 2H), 7.57 (d, J=7.6 Hz, 1H), 5.37-5.43 (m, 1H), 5.06 (d, J=16.0 Hz, 1H), 4.90-4.93 (m, 1H), 4.65 (d, J=11.6 Hz, 1H), 3.60-3.74 (m, 2H), 3.34-3.42 (m, 1H), 3.22 (dd, J=14.8, 7.6 Hz, 2H), 3.19-3.26 (m, 2H), 1.88-2.27 (m, 10H), 1.39-1.54 (m, 2H), 1.15-1.28 (m, 8H). 19F NMR (CD3OD 400 MHz): δ −75.40. Isomer SFC tR=1.860 min in 3.0 min chromatography (AD-H_3 UM_3_5_40_4 mL, ee=95.93%).
Isomer 2 (25b) was prepared according to Scheme 25 using (R)-2-(tert-butoxycarbonyl)-1-ethylisoindoline-5-carboxylic acid as the starting material. LC-MS tR=0.814 min in 10-80AB_2.0 min chromatography (Welch Xtimate C18, 2.1*30 mm, 3 μm), MS (ESI) m/z 581.2 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 7.94-7.98 (m, 1H), 7.88-7.93 (m, 3H), 7.68-7.73 (m, 2H), 7.54-7.59 (m, 1H), 5.35-5.44 (m, 1H), 5.01-5.10 (m, 1H), 4.91-4.95 (m, 1H), 4.60-4.68 (m, 1H), 3.60-3.74 (m, 2H), 3.34-3.41 (m, 1H), 3.26-3.32 (m, 1H), 3.22 (dd, J=14.8, 7.6 Hz, 2H), 1.95-2.27 (m, 10H), 1.38-1.55 (m, 2H), 1.03-1.32 (m, 8H). 19F NMR (CD3OD 400 MHz): δ −75.39. Isomer SFC tR=1.860 min in 3.0 min chromatography (AD-H_3 UM_3_5_4 mL, ee=98.71%).
Examples 26a and 26b
N-(4-(ethylsulfonyl)benzyl)-1-isopropyl-3-oxo-2-(4-(trifluoromethyl)benzyl)isoindoline-5-carboxamide Isomers








To a suspension of 2-methylpropane-2-sulfinamide (758 mg, 6.25 mmol) in DCM (15 mL) was added CuSO4 (2.2 g, 13.75 mmol) followed by isobutyraldehyde (497 mg, 6.9 mmol) in one portion at rt. The mixture was allowed to stir for 10 h at rt. Vacuum filtration was used to remove the solid and the filtrate was dried over Na2SO4, filtered and concentrated under reduced pressure to afford crude (E)-2-methyl-N-(2-methylpropylidene)propane-2-sulfinamide as a white solid, which was used directly for the next step without further purification.
To a solution of 2-bromo-5-chlorobenzoic acid (505 mg, 2.15 mmol) in dry THF (20 mL) was added a 2.5 M solution of n-BuLi in THF (2.2 mL, 5.4 mmol) slowly under N2 at −78° C. The mixture was allowed to stir for 10 min at −78° C. then a solution of the above crude (E)-2-methyl-N-(2-methylpropylidene)propane-2-sulfinamide (395 mg, 2.26 mmol) in THF (5 mL) was introduced to the reaction mixture. The reaction was allowed to stir for 10 min at −78° C. then slowly allowed to warm to rt. H2O (20 mL) was added at rt and the mixture was washed with EtOAc (30 mL). The aqueous phase was acidified with 1 N HCl solution to pH=3 and extracted with EtOAc (2×30 mL). The organic layers were combined, dried over Na2SO4 and evaporated to afford crude product. Purification using flash chromatography over silica gel (eluting with 5% MeOH in DCM) gave 200 mg of 2-(1-((tert-butylsulfinyl)amino)-2-methylpropyl)-5-chlorobenzoic acid as a colorless oil (30% yield). LC-MS tR=1.45 min in 2 min chromatography, MS (ESI) m/z 332.1 [M+H]+.
To a solution of compound 2-(1-((tert-butylsulfinyl)amino)-2-methylpropyl)-5-chlorobenzoic acid (200 mg, 0.6 mmol) in CH2Cl2 (2 mL) was added HCl in methanol (0.5 M, 3 mL, 1.5 mmol) at 0° C. The mixture was stirred at 0° C. for 2 h. Et3N was added and the mixture was concentrated under reduced pressure to afford crude 2-(1-amino-2-methylpropyl)-5-chlorobenzoic acid, which was used for the next step directly without further purification.
To a solution of crude 2-(1-amino-2-methylpropyl)-5-chlorobenzoic acid (0.6 mmol) and iPr2NEt (420 μL, 2.4 mmol) in THF (3 mL) was added HATU (340 mg, 0.9 mmol) at rt. The reaction was stirred for 1 h before adding a sat. NH4Cl solution (10 mL). The mixture was extracted with EtOAc (2×10 mL). The organic layers were combined, washed with brine (10 mL), dried over Na2SO4, filtered and concentrated under reduced pressure. The crude material was purified by flash chromatography over silica gel (eluting with 30% EtOAc in hexanes) to afford 108 mg of 6-chloro-3-isopropylisoindolin-1-one as a colorless oil (86% yield). LC-MS tR=1.23 min in 2 min chromatography, MS (ESI) m/z 210.1 [M+H]+. 1H NMR (CD3OD) δ 7.72 (s, 1H), 7.62 (d, J=8 Hz, 1H), 7.55 (d, J=8 Hz, 1H), 4.61 (d, J=2.8 Hz, 1H), 2.32-2.28 (m, 1H), 1.05 (d, J=6.8 Hz, 1H), 0.69 (d, J=6.8 Hz, 1H).
To a solution of 6-chloro-3-isopropylisoindolin-1-one (91 mg, 0.44 mmol) in DMF (3 mL) was added NaH (35 mg, 0.87 mmol) at 0° C. and the mixture was stirred at 0° C. for 15 mins. 1-(Bromomethyl)-4-(trifluoromethyl)benzene was added in one portion and the resulting mixture was allowed to stir at 0° C. for 30 min. A saturated NH4Cl solution (10 mL) was then added and the mixture was extracted with EtOAc (2×10 mL). The organic layers were combined, washed with brine (10 mL), dried over Na2SO4, filtered and concentrated under reduced pressure. The crude mixture was purified by flash chromatography over silica gel (eluting with 30% EtOAc in hexanes) to afford 90 mg of 6-chloro-3-isopropyl-2-(4-(trifluoromethyl)benzyl)isoindolin-1-one as a colorless oil (56% yield). LC-MS tR=1.87 min in 2 min chromatography, MS (ESI) m/z 368.1 [M+H]+.
To a solution of 6-chloro-3-isopropyl-2-(4-(trifluoromethyl)benzyl)isoindolin-1-one (95 mg, 260 μmol) in dioxane/H2O (2/0.2 mL) was added molybdenum hexacarbonyl (42 mg, 156 μmol) and 1,8-diazabicyclo[5.4.0]undec-7-ene (102 μL, 780 μmol) in a microwave tube. The mixture was degassed with N2 for 5 min, at which point trans-bis(acetato)bis[o-(di-o-tolylphosphino)benzyl]dipalladium(II) (13 mg, 13 μmol) and tri-tert-butylphosphonium tetrafluoroborate (8 mg, 26 zμmol) were added. The mixture was degassed again with N2 for 10 min then heated in a CEM microwave reactor at 160° C. for 20 min. After cooling to rt, the mixture was diluted with H2O (20 mL) and washed with EtOAc (20 mL). The aqueous phase was acidified with 1 N HCl solution to pH=3 and extracted with EtOAc (3×20 mL). The organic layer was washed with brine, dried over Na2SO4, and concentrated to afford crude 1-isopropyl-3-oxo-2-(4-(trifluoromethyl)benzyl)isoindoline-5-carboxylic acid (670 mg, 70%) as a light yellow solid. This material was used for the next step without further purification.
To a solution of 1-isopropyl-3-oxo-2-(4-(trifluoromethyl)benzyl)isoindoline-5-carboxylic acid (30 mg, 100 μmol) in DMF (2 mL) was added (4-(ethylsulfonyl)phenyl)methanamine HCl salt (30 mg, 100 μmol), HATU (57 mg, 100 μmol) and DIEA (70 μL, 400 μmol). The mixture was stirred at rt for 4 h. Ethyl acetate (20 mL) and water (20 mL) were added to the mixture. After partition, the aqueous layer was extracted with ethyl acetate (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The solvent was removed in vacuo to give the crude product which was purified by Gilson-HPLC to afford N-(4-(ethylsulfonyl)benzyl)-1-isopropyl-3-oxo-2-(4-(trifluoromethyl)benzyl)isoindoline-5-carboxamide. The racemic mixture was purified by SFC separation followed by basic preparative HPLC separation to afford isomer two isomers (26a and 26b) as white solids.
Isomer 1 (26a) SFC tR=1.916 min in 3 min chromatography (ee=92%); LC-MS tR=0.774 min in 5-95AB_1.5 min chromatography (Welch MK RP-18e 25-2 mm), MS (ESI) m/z 559.0 [M+H]+; 1H NMR (CDCl3, 400 MHz): δ 8.24 (s, 1H), 8.17 (d, J=8.0 Hz, 1H), 7.90 (d, J=8.4 Hz, 2H), 7.61-7.55 (m, 5H), 7.38 (d, J=8.0 Hz, 2H), 6.84 (t, J=6.0 Hz, 1H), 5.43 (d, J=15.6 Hz, 1H), 4.78 (d, J=6.4 Hz, 2H), 4.35 (d, J=3.6 Hz, 1H), 4.25 (d, J=15.6 Hz, 1H), 3.13 (q, J=7.6 Hz, 2H), 2.48-2.39 (m, 1H), 1.29 (t, J=7.6 Hz, 3H), 1.20 (d, J=6.8 Hz, 3H), 0.51 (d, J=6.8 Hz, 3H).
Isomer 2 (26b) SFC tR=0.542 min in 3 min chromatography (ee=88%); LC-MS tR=0.772 min in 5-95AB_1.5 min chromatography (Welch MK RP-18e 25-2 mm), MS (ESI) m/z 559.0 [M+H]+; 1H NMR (CDCl3, 400 MHz): δ 8.24 (s, 1H), 8.17 (d, J=8.4 Hz, 1H), 7.90 (d, J=8.4 Hz, 2H), 7.61-7.55 (m, 5H), 7.38 (d, J=8.0 Hz, 2H), 6.84 (t, J=6.0 Hz, 1H), 5.43 (d, J=15.6 Hz, 1H), 4.78 (d, J=6.0 Hz, 2H), 4.35 (d, J=3.2 Hz, 1H), 4.25 (d, J=15.6 Hz, 1H), 3.13 (q, J=7.6 Hz, 2H), 2.48-2.39 (m, 1H), 1.29 (t, J=7.6 Hz, 3H), 1.20 (d, J=6.8 Hz, 3H), 0.51 (d, J=6.8 Hz, 3H).
The following compounds in Table 3 were prepared using the methods described in Scheme 26 with 1-isopropyl-3-oxo-2-(4-(trifluoromethyl)benzyl)isoindoline-5-carboxylic acid and the appropriate ethylsulfonyl amine.










TABLE 3





Ex.





No.
Structure
LCMS
1H-NMR


















27




560.3 [M + H]+
(CD3OD, 400 MHz): δ 8.99 (s, 1H), 8.36 (s, 1H), 8.26 (d, J = 8.4 Hz, 1H), 8.16 (d, J = 8.4 Hz, 1H), 7.72-7.65 (m, 5H), 7.51 (d, J = 8.4 Hz, 2H), 5.28 (d, J = 15.6 Hz, 1H), 4.80 (s, 2H), 4.56-4.53 (m, 2H), 3.29 (q, J = 7.6 Hz, 2H), 2.54-2.51 (m, 1H), 1.25 (t, J = 6.8 Hz, 3H), 1.22 (t, J = 6.8 Hz, 3H), 0.48 (d, J = 6.8 Hz, 3H).


 


28




589.3 [M + H]+
(CD3OD, 400 MHz): δ 8.36 (s, 1H), 8.13 (d, J = 8.4 Hz, 1H), 7.90 (d, J = 8.0 Hz, 2H), 7.72-7.65 (m, 5H), 7.51 (d, J = 8.4 Hz, 2H), 5.28 (d, J = 15.6 Hz, 1H), 4.54 (t, J = 8.0 Hz, 2H), 3.93 (d, J = 6.4 Hz, 2H), 3.19 (q, J = 7.6 Hz, 2H), 2.55-2.50 (m, 1H), 1.27-1.19 (m, 6H), 0.47 (d, J = 6.8 Hz, 3H).









Example 29
(S)—N-(4-(ethylsulfonyl)benzyl)-1-isopropyl-3-oxo-2-((trans-4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxamide (29)




To a solution of 2-bromo-5-chlorobenzoic acid (3.33 g, 14 mmol) in dry THF (100 mL) was added a 2.5 M solution of n-BuLi in THF (14 mL, 35.5 mmol) slowly under N2 at −78° C. The mixture was allowed to stir for 10 min at −78° C. then a solution of (S)-2-methyl-N-(2-methylpropylidene)propane-2-sulfinamide (2.48 g, 14 mmol) in THF (10 mL) was added. The reaction was allowed to stir for 10 min at −78° C. then slowly warmed to rt. The reaction was quenched by adding H2O (50 mL) and the mixture was washed with EtOAc (50 mL). The aqueous phase was acidified with 1 N HCl solution to pH=3 and extracted with EtOAc (2×50 mL). The organic layers were combined, dried over Na2SO4 and evaporated to afford crude product, which was purified by flash chromatography over silica gel (eluting with 5% MeOH in DCM) to afford 925 mg of 2-((S)-1-(((S)-tert-butylsulfinyl)amino)-2-methylpropyl)-5-chlorobenzoic acid as an off-white solid (20% yield). LC-MS tR=2.87 min in 5 min chromatography, MS (ESI) m/z 332.4 [M+H]+.
To a solution of 2-((S)-1-(((S)-tert-butylsulfinyl)amino)-2-methylpropyl)-5-chlorobenzoic acid (25 mg, 76 μmol) in CH2Cl2 (0.5 mL) was added HCl in methanol (0.5 M, 0.3 mL, 150 μmol) at 0° C. The mixture was stirred at 0° C. for 1 h. The mixture was concentrated under reduced pressure to afford crude (S)-2-(1-amino-2-methylpropyl)-5-chlorobenzoic acid HCl salt, which was used for the next step directly without further purification. LC-MS tR=0.70 min in 2 min chromatography, MS (ESI) m/z 228.2 [M+H]+.
To a solution of (S)-2-(1-amino-2-methylpropyl)-5-chlorobenzoic acid HCl salt (76 μmol) in dichloromethane (0.5 mL) was added solid NaHCO3 (10 mg) and the mixture was stirred for 5 min at rt. Trans-4-(trifluoromethyl)cyclohexane-1-carbaldehyde (22 mg, 110 μmol) was added followed by NaCNBH3 (12 mg, 190 μmol). The mixture was stirred at rt for 1 h. Ethyl acetate (5 mL) and water (5 mL) were added to the mixture. After partition, the aqueous layer was extracted with ethyl acetate (2×5 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The solvent was removed in vacuo to give crude 5-chloro-2-((S)-2-methyl-1-(((trans-4-(trifluoromethyl)cyclohexyl)methyl)amino)propyl)benzoic acid which was used for the next step directly without further purification. LCMS tR=1.29 min in 2 min chromatography, MS (ESI) m/z 392.2 [M+H]+.
To a solution of 5-chloro-2-(((S)-2-methyl-1-(((trans-4-(trifluoromethyl)cyclohexyl)methyl)amino)propyl)benzoic acid (76 μmol) in THF (0.5 mL) was added HATU (42 mg, 110 μmol) and DIEA (40 μL, 200 μmol). The mixture was stirred at rt for 2 h. Ethyl acetate (5 mL) and water (5 mL) were added to the mixture. After partition, the aqueous layer was extracted with ethyl acetate (2×5 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The solvent was removed in vacuo to give the crude product which was purified by flash chromatography over silica gel (eluting with 20% EtOAc in hexanes) to afford 5 mg of (S)-6-chloro-3-isopropyl-2-((trans-4-(trifluoromethyl)cyclohexyl)methyl)isoindolin-1-one as a colorless oil. LC-MS tR=1.90 min in 2 min chromatography, MS (ESI) m/z 374.3 [M+H]+.
To a solution of (S)-6-chloro-3-isopropyl-2-((trans-4-(trifluoromethyl)cyclohexyl)methyl)isoindolin-1-one (5 mg, 13 μmol) in dioxane/H2O (200/20 μL) was added molybdenum hexacarbonyl (3 mg, 10 μmol) and 1,8-diazabicyclo[5.4.0]undec-7-ene (6 μL, 39 μmol) in a microwave tube. The mixture was degassed with N2 for 5 min, at which point trans-Bis(acetato)bis[o-(di-o-tolylphosphino)benzyl]dipalladium(II) (3 mg, 2.6 μmol) and tri-tert-butylphosphonium tetrafluoroborate (2 mg, 5.2 μmol) were added. The mixture was degassed again with N2 for 10 min then heated in a CEM microwave reactor at 160° C. for 20 min. After cooling to rt, the mixture was diluted with H2O (5 mL) and washed with EtOAc (5 mL). The aqueous phase was acidified with 1 N HCl solution to pH=3 and extracted with EtOAc (3×5 mL). The organic layer was washed with brine, dried over Na2SO4, and concentrated to afford (S)-1-isopropyl-3-oxo-2-((trans-4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxylic acid as a colorless oil. This material was used for the next step directly without further purification. LC-MS tR=1.61 min in 2 min chromatography, MS (ESI) m/z 384.2 [M+H]+.
To a solution of (S)-1-isopropyl-3-oxo-2-((trans-4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxylic acid (5 mg, 13 μmol) in DMF (0.2 mL) was added (4-(ethylsulfonyl)phenyl)methanamine (4 mg, 20 μmol), HATU (8 mg, 20 μmol) and DIEA (7 μL, 40 μmol). The mixture was stirred at rt for 4 h. Ethyl acetate (3 mL) and water (3 mL) were added to the mixture. After partition, the aqueous layer was extracted with ethyl acetate (2×3 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The solvent was removed in vacuo to give the crude product which was purified on Gilson-HPLC to afford (S)—N-(4-(ethylsulfonyl)benzyl)-1-isopropyl-3-oxo-2-((trans-4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxamide (29). LC-MS tR=1.62 min in 2 min chromatography, MS (ESI) m/z 565.4 [M+H]+. 1H NMR (CD3OD, 400 MHz): δ 9.32 (m, 1H), 8.25 (s, 1H), 8.25 (s, 1H), 8.12 (d, J=7.6 Hz, 1H), 7.88 (d, J=8.8 Hz, 2H), 7.74 (d, J=8.0 Hz, 1H), 7.64 (d, J=8.8 Hz, 2H), 4.74-4.71 (m, 4H), 3.88-3.82 (m, 1H), 3.21 (q, J=7.6 Hz, 2H), 3.14-3.09 (m, 1H), 2.55-2.53 (m, 1H), 2.52-1.67 (m, 9H), 1.28 (d, J=6.8 Hz, 3H), 1.23 (t, J=6.8 Hz, 3H), 0.47 (d, J=6.8 Hz, 3H).
Example 30
N-(4-(ethylsulfonyl)benzyl)-1,1-dimethyl-3-oxo-2-((4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxamide (30)




To a solution of (4-(trifluoromethyl)cyclohexyl)methanamine (340 mg, 1.88 mmol) in dichloromethane (5 mL) was added acetone (165 μL, 2.25 mmol) followed by NaCNBH3 (240 mg, 3.76 mmol). The resulting mixture was stirred at rt for 8 h. The reaction was quenched with a saturated NaHCO3 solution (5 mL) and extracted with DCM (6×10 mL). The combined organic layers were dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure to afford N-((4-(trifluoromethyl)cyclohexyl)methyl)propan-2-amine (250 mg) as a clear oil which was used for the next step directly without further purification. LC-MS tR=0.73 min in 2 min chromatography, MS (ESI) m/z 224.2 [M+H]+.
To a solution of N-((4-(trifluoromethyl)cyclohexyl)methyl)propan-2-amine (250 mg, 1.07 mmol) in DMF (4 mL) was added 5-chloro-2-iodobenzoic acid (395 mg, 1.4 mmol), HATU (610 mg, 1.6 mmol) and DIEA (480 μL, 2.7 mmol). The mixture was stirred at rt for 3 h. Ethyl acetate (10 mL) and water (10 mL) were added to the mixture. After partition, the aqueous layer was extracted with ethyl acetate (2×10 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The solvent was removed in vacuo to give the crude product which was purified by flash chromatography over silica gel (eluting with 15% EtOAc in hexanes) to afford 420 mg of 5-chloro-2-iodo-N-isopropyl-N-((4-(trifluoromethyl)cyclohexyl)methyl)benzamide as a clear oil (86% yield). LC-MS tR=1.99 min in 2 min chromatography, MS (ESI) m/z 488.2 [M+H]+.
To a solution of 5-chloro-2-iodo-N-isopropyl-N-((4-(trifluoromethyl)cyclohexyl)methyl)benzamide (130 mg, 0.27 mmol) in benzene (3 mL) was added AIBN (25 mg, 27 μmol) followed by potassium tert-butoxide (91 mg, 0.81 mmol). The resulting solution was heated to 80° C. for 8 h. After cooling down to rt, water (10 mL) was added to the reaction mixture. The resulting solution was extracted with ethyl acetate (2×10 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The resulting crude reaction mixture was purified by flash chromatography over silica gel (eluting with 15% EtOAc in hexanes) to afford 70 mg of 6-chloro-3,3-dimethyl-2-((4-(trifluoromethyl)cyclohexyl)methyl)isoindolin-1-one as a clear oil (70% yield). LC-MS tR=1.83, 1.89 min in 2 min chromatography, MS (ESI) m/z 360.2 [M+H]+.
To a solution of 6-chloro-3,3-dimethyl-2-((4-(trifluoromethyl)cyclohexyl)methyl)isoindolin-1-one (70 mg, 190 μmol) in dioxane/H2O (2/0.2 mL) was added molybdenum hexacarbonyl (40 mg, 152 μmol) and 1,8-diazabicyclo[5.4.0]undec-7-ene (75 μL, 570 μmol) in a microwave tube. The mixture was degassed with N2 for 5 min, at which point trans-Bis(acetato)bis[o-(di-o-tolylphosphino)benzyl]dipalladium(II) (18 mg, 19 μmol) and tri-tert-butylphosphonium tetrafluoroborate (22 mg, 38 μmol) were added. The tube was degassed with N2 again for 10 min then heated in a CEM microwave reactor at 160° C. for 20 min. After cooling to rt, the mixture was diluted with H2O (5 mL) and washed with EtOAc (5 mL). The aqueous phase was acidified with 1 N HCl solution to pH=3 and extracted with EtOAc (3×5 mL). The organic layer was washed with brine, dried over Na2SO4, and concentrated to afford crude 1,1-dimethyl-3-oxo-2-((4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxylic acid as a colorless oil. This material was used for the next step without further purification.
To a solution of 1,1-dimethyl-3-oxo-2-((4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxylic acid (10 mg, 27 μmol) in DMF (0.25 mL) was added (4-(ethylsulfonyl)phenyl)methanamine (7 mg, 35 μmol), HATU (16 mg, 41 μmol) and DIEA (12 μL, 68 μmol). The mixture was stirred at rt for 4 h. Ethyl acetate (3 mL) and water (3 mL) were added to the mixture. After partition, the aqueous layer was extracted with ethyl acetate (2×3 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, filtered and concentrated under reduced pressure. The solvent was removed in vacuo to give the crude product which was purified on Gilson-HPLC to afford N-(4-(ethylsulfonyl)benzyl)-1,1-dimethyl-3-oxo-2-((4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxamide (30). LC-MS tR=1.52 min in 2 min chromatography, MS (ESI) m/z 551.4 [M+H]+. 1H NMR (CD3OD, 400 MHz): δ 9.18 (m, 1H), 7.91 (d, J=8.8 Hz, 2H), 7.85 (d, J=7.6 Hz, 1H), 7.70-7.62 (m, 3H), 7.58 (t, J=7.6 Hz, 1H), 4.91-4.89 (m, 1H), 4.80-4.77 (m, 1H), 4.70-4.66 (m, 2H), 3.52 (m, 1H), 3.40-3.35 (m, 1H), 3.21 (q, J=7.6 Hz, 2H), 2.20-2.18 (m, 1H), 1.97-1.68 (m, 7H), 1.59 (s, 6H), 1.30-1.27 (m, 1H), 1.21 (t, J=6.8 Hz, 3H).
Example 31
1-ethyl-N-(4-(ethylsulfonyl)benzyl)-1-methyl-3-oxo-2-((4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxamide (31)




1-ethyl-N-(4-(ethylsulfonyl)benzyl)-1-methyl-3-oxo-2-((4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxamide (31) was prepared in accordance with Scheme 28, using 1,1-dimethyl-3-oxo-2-((4-(trifluoromethyl)cyclohexyl)methyl)isoindoline-5-carboxylic acid. LCMS 565.4 [M+H]+; 1H-NMR (CD3OD, 400 MHz): δ 9.18 (m, 1H), 7.91 (d, J=8.4 Hz, 2H), 7.86 (d, J=7.6 Hz, 1H), 7.70-7.61 (m, 3H), 7.59 (t, J=7.6 Hz, 1H), 4.91-4.89 (m, 1H), 4.83-4.81 (m, 1H), 4.70-4.60 (m, 2H), 3.65-3.60 (m, 1H), 3.31-3.27 (m, 1H), 3.21 (q, J=7.6 Hz, 2H), 2.39-2.32 (m, 1H), 2.20-2.12 (m, 2H), 2.01-1.90 (m, 4H), 1.71-1.68 (m, 2H), 1.59 (s, 3H), 1.55 (m, 2H), 1.16 (t, J=6.8 Hz, 3H), 0.28 (m, 3H).
Examples 32a and 32b
(S)—N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropyl-2-((2-(trifluoromethyl)-1,3-dioxan-5-yl)methyl)isoindoline-5-carboxamide (32a and 32b)








To a solution of propane-1,2,3-triol (1.0 g, 9.43 mmol) and imidazole (565 mg, 8.31 mmol) in dry DMF (7 mL) was added TBDPSCl (1 mL, 3.86 mmol) at rt. The reaction was stirred for 24 h at rt. Water (10 mL) and EtOAc (30 mL) were added after 24 h. The water layer was separated and extracted twice with EtOAc (2×15 mL). The EtOAc layers were combined, dried using Na2SO4, and evaporated. The crude residue was purified by ISCO flash column chromatography (eluting with a gradient of 0% to 50% EtOAc in hexanes) to afford 3-((tert-butyldiphenylsilyl)oxy)propane-1,2-diol (950 mg, 72%) as a clear colorless oil. LC-MS tR=1.728 min in 2 min chromatography, MS (ESI) m/z 345.36 [M+H]+.
To a suspension of 3-((tert-butyldiphenylsilyl)oxy)propane-1,2-diol (500 mg, 1.45 mmol), KI (60 mg, 0.36 mmol) and Ag2O (673 mg, 2.90 mmol) in DCM (6 mL) was added TsCl (277 mg, 1.45 mmol) at rt. The reaction was stirred at rt for 24 h. Filtration through Celite® and evaporation gave the crude product. Purification using ISCO flash column chromatography (eluting with a gradient of 0% to 50% EtOAc in hexanes) gave 3-((tert-butyldiphenylsilyl)oxy)-2-hydroxypropyl 4-methylbenzenesulfonate (560 mg, 78%). LC-MS tR=2.089 min in 2 min chromatography, MS (ESI) m/z 499.39 [M+H]+.
Gaseous 2,2,2-trifluoroacetaldehyde was bubbled into a solution of 3-((tert-butyldiphenylsilyl)oxy)-2-hydroxypropyl 4-methylbenzenesulfonate (192 mg, 0.39 mmol) in dry THF (6 mL) at rt. The gaseous 2,2,2-trifluoroacetaldehyde was generated in a separated flask by the dropwise addition of 2,2,2-trifluoro-1-methoxyethan-1-ol (1 mL) into polyphosphoric acid at 90° C. One end of a Teflon® tubing was placed inside of the sealed flask, while the other end was placed into the solution of 3-((tert-butyldiphenylsilyl)oxy)-2-hydroxypropyl 4-methylbenzenesulfonate in THF at rt. When the bubbling subsided in the reaction, the mixture was stirred at rt for 15 h, at which point LCMS analysis showed complete consumption of the 3-((tert-butyldiphenylsilyl)oxy)-2-hydroxypropyl 4-methylbenzenesulfonate and formation of 3-((tert-butyldiphenylsilyl)oxy)-2-((2,2,2-trifluoro-1-hydroxyethoxy)methyl)propyl 4-methylbenzenesulfonate (structure not shown; LC-MS tR=2.170 min in 2 min chromatography, MS (ESI) m/z 597.45 [M+H]+). Solid K2CO3 was then added and the reaction was stirred at 70° C. for 6 h, at which point LCMS analysis showed consumption of the 3-((tert-butyldiphenylsilyl)oxy)-2-((2,2,2-trifluoro-1-hydroxyethoxy)methyl)propyl 4-methylbenzenesulfonate and formation of a new, less polar product, which was observed on the LCMS from the UV-vis spectrum (wavelength=220 nM), but showed no ionization peak.
Water (10 mL) and EtOAc (20 mL) were added after cooling the reaction to rt. The water layer was extracted with EtOAc (10 mL). The EtOAc layers were combined and washed with sat. NH4Cl (5 mL), then sat. NaHCO3 (5 mL). The EtOAc solution was then dried using Na2SO4 and evaporated to give crude tert-butyldiphenyl((2-(trifluoromethyl)-1,3-dioxan-5-yl)methoxy)silane (190 mg), which was used directly for the next step without purification. LC-MS tR=2.214 min in 2 min chromatography, no ionization peak (only UV-vis peak was observed at 220 nM).
To a solution of tert-butyldiphenyl((2-(trifluoromethyl)-1,3-dioxan-5-yl)methoxy)silane (crude 190 mg from previous step) in EtOH (2.5 mL) was added 5N NaOH (0.50 mL). The reaction was heated to 75° C. for 3 h. The EtOH was removed by rotovap and DCM (10 mL) was added, followed by 1N HCl (3 mL). The aqueous layer was separated and extracted using DCM (2×5 mL). The DCM layers were combined, dried using Na2SO4, and evaporated to give crude (2-(trifluoromethyl)-1,3-dioxan-5-yl)methanol containing TBDPS-OH, from LCMS analysis. This material was used directly for the next step without further purification. LC-MS tR=1.168 min in 2 min chromatography, MS (ESI) m/z 187.13 [M+H]+.
To a solution of crude (2-(trifluoromethyl)-1,3-dioxan-5-yl)methanol (23 mg, from previous step) in DCM (2 mL) was added a solid mixture of PCC (53 mg, 0.25 mmol) and Celite® (50 mg). The suspension was stirred at rt for 3 h. Et2O (5 mL) was added to the mixture at rt and stirring continued for 30 min. The suspension was then filtered through a pad of Celite® and evaporated to give crude 2-(trifluoromethyl)-1,3-dioxane-5-carbaldehyde, which was used for the next step without purification. 19F NMR showed a 4:1 mixture of acetal isomers from the 2 doublet signals of the CF3 group. LC-MS (no ionizable or UV-vis peak detected on the LCMS). 19F NMR (CDCl3, 400 MHz): δ −82.90 ppm (major isomer), −82.37 ppm (minor isomer).
Isomers 1 and 2 (32a and 32b) were prepared according to General Procedure A, using (S)—N-((5-(ethylsulfonyl)pyridin-2-yl)methyl)-1-isopropylisoindoline-5-carboxamide and 2-(trifluoromethyl)-1,3-dioxane-5-carbaldehyde (4:1 mixture of acetal isomers) as the starting materials. The crude material was separated by SFC (IC-3) to give two crude products, which were purified by HCl preparative HPLC separation, then lyophilized directly to Isomer 2 (32b) HCl salt (major isomer from 4:1 CF3-acetal mixture) and isomer 1 (32a) HCl salt (minor isomer from 4:1 CF3-acetal mixture) as white solids. Based on the biological activity of the two isomers it was determined that isomer 2 was the major product from the 4:1 mixture of 2-(trifluoromethyl)-1,3-dioxane-5-carbaldehyde.
Isomer 2 (32b) HCl salt (major isomer from 4:1 acetal mixture). LC-MS tR=0.632 min in 5-95AB_1.5 min chromatography (Welch MK RP-18e 25-2 mm), MS (ESI) m/z 556.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 9.08 (d, J=2.0 Hz, 1H), 8.47 (dd, J=2.4, 8.4 Hz, 1H), 8.04 (d, J=8.4 Hz, 1H), 8.02 (s, 1H), 7.85 (d, J=8.0 Hz, 1H), 7.60 (d, J=8.4 Hz, 1H), 5.18 (d, J=2.8 Hz, 1H), 5.15 (s, 1H), 5.09 (d, J=4.8 Hz, 1H), 4.89 (s, 2H), 4.80-4.75 (m, 1H), 4.39-4.23 (m, 4H), 3.80 (d, J=6.0 Hz, 2H), 3.36 (q, J=7.6 Hz, 2H), 2.50 (bs, 1H), 2.32 (bs, 1H), 1.29 (t, J=7.6 Hz, 3H), 1.23 (d, J=6.8 Hz, 3H), 1.08 (d, J=6.8 Hz, 3H). Isomer 1 (32a) HCl salt (minor isomer from 4:1 acetal mixture). LC-MS tR=0.638 min in 5-95AB_1.5 min chromatography (Welch MK RP-18e 25-2 mm), MS (ESI) m/z 556.1 [M+H]+. 1H NMR (CD3OD 400 MHz): δ 9.02 (d, J=2.0 Hz, 1H), 8.33 (dd, J=2.4, 8.4 Hz, 1H), 8.03 (d, J=8.4 Hz, 1H), 8.00 (s, 1H), 7.72 (d, J=8.0 Hz, 1H), 7.60 (d, J=8.4 Hz, 1H), 5.15-5.01 (m, 3H), 4.84 (s, 2H), 4.68-4.64 (m, 1H), 4.48-4.40 (m, 2H), 3.79-3.68 (m, 2H), 3.36 (q, J=7.6 Hz, 2H), 3.29-3.22 (m, 2H), 2.68 (bs, 1H), 2.47 (bs, 1H), 1.27 (t, J=7.6 Hz, 3H), 1.23 (d, J=6.8 Hz, 3H), 1.03 (d, J=6.8 Hz, 3H).
For the above example, it will be understood that the “*” indicates that although a single diastereomer was isolated, the absolute configuration about these positions was not fully characterized, however the relative stereochemistry at one of the designated positions to the other designated position is as shown. Accordingly, in each of compounds 32a and 32b, a single diastereomer was isolated and tested, but the absolute stereochemistry about the “*” is arbitrarily defined.
BIOLOGICAL ASSAYS
Radio-Ligand RORγ Binding Assay (Assay 1)
Compounds of the present invention were tested for ability to bind to RORγ in a cell-free competition assay with commercially available radio-ligand (RL), 25-hydroxy[26,27-3H]-cholesterol (PerkinElmer, Cat. #NET674250UC), for a ligand binding site on a recombinant RORγ Ligand Binding Domain (LBD) protein expressed as a 6×His-Glutathione-S-Transferase (GST) fusion. The assay was performed in 96-well SPA plates (PerkinElmer, Cat. #1450-401) in 50 mM HEPES buffer, pH 7.4, containing 150 mM NaCl, 5 mM MgCl2, 10% (v/v) glycerol, 2 mM CHAPS, 0.5 mM β-octylglucopyranoside and 5 mM DTT. Tested compounds were dissolved in DMSO, and semi-log (3.162×) serial dilutions of the compounds were prepared in the same solvent. Two μL of the DMSO solutions were mixed with 28 μL of 8.6 nM 25-hydroxy[26,27-3H]-cholesterol and 50 μL of 24 nM RORγ LBD. The plate was shaken at 700 rpm for 20 min and incubated for 10 min at rt, after which 40 μL of poly-Lys YSi SPA beads (PerkinElmer, Cat. #RPNQ0010) were added to achieve 50 μg of the beads per well. The plate was incubated on an orbital shaker for 20 min and then for 10 min without agitation at rt. SPA signal for tritium beta radiation was registered on PerkinElmer Microbeta plate reader. Percent inhibition values were calculated based on the high signal obtained with DMSO control and the low signal observed with 10 μM standard RORγ inverse agonist T0901317 (SigmaAldrich, Cat. #T2320). The percent inhibition vs. concentration data were fit into a four-parameter model, and IC50 values were calculated from the fit as the concentrations corresponding to the inflection points on the dose-response curves. Inhibitory constants (Ki) were calculated using the following equation, where [RL] is the concentration in the assay and KD is a dissociation constant of 25-hydroxy[26,27-3H]-cholesterol:




  
    K
    i
  
  =
  
    
      
        I
        ⁢
        
          
        
        ⁢
        
          C
          50
        
      
      
        (
        
          1
          +
          
            
              [
              
                R
                ⁢
                
                  
                
                ⁢
                L
              
              ]
            
            
              K
              D
            
          
        
        )
      
    
    .
  




RORγt 5×RORE Assay in Jurkat Cells (Assay 2)

Compounds of the present invention were tested for RORγ inverse agonist activity in a cell-based, transcriptional activity assay. Secreted Nanoluc® luciferase was used as a reporter for transcriptional activity of the full-length RORγt in Jurkat cells (ATCC, Cat. #TIB-152). A reporter plasmid was constructed by inserting 5 repeats of the ROR Response Element (RORE) AAAGTAGGTCA (SEQ ID NO:1) into a commercially available promoterless plasmid pNL1.3[secNluc] (Promega, Cat. #N1021) using KpnI and HindIII restriction sites. The expression plasmid for RORγt was purchased (GeneCopoeia, Cat. #EX-T6988-M02). Jurkat cells (30 million cells) were transfected with 11 μg of EX-T6988-MO2 and 26 μg of the reporter plasmid in OptiMEM® media using Lipofectamine® LTX and PlusTM reagents (Life Technologies, Cat. #15338-100). After 5-6 hr incubation at 37° C./5% CO2, the cells were collected, resuspended in phenol-red free RPMI media containing 10% (v/v) delipidated FBS (Hyclone, Cat. #SH30855.03) and dispensed into 96-well clear bottom tissue culture plates (CoStar, Cat. #3603), at 80,000 cells per well. Tested compounds were added to the cells in the same media (final concentration of DMSO was 0.1% (v/v)), and the plates were incubated at 37° C./5% CO2 for 16-18 hrs. Luciferase activity in the conditioned supernatants was determined with NanoGlo® assay reagents (Promega, Cat.#N1130). Percent inhibition values were calculated based on the fully inhibited and non-inhibited (DMSO) controls, and the values were regressed against concentrations of the tested compounds to derive IC50 values using a four-parameter non-linear fitting model.
The results of assays 1 and 2 are shown in Table 4.











TABLE 4








ROR  





Binding
ROR   t5X




Ki
IC50




Range*
Range*



Compound
(nM)
(nM)



Number
(Assay 1)
(Assay 2)









 1a
+++
+++



 1b
++
++



 2a
+++
+++



 2b
+++
+



 2c
+++
+++



 2d
++



 3a
+++
+++



 3b
+++
++



 3c
+++
+++



 3d
+++
+



 4a
+++
+++



 4b
++



 5a
+++
+++



 5b
++



 6a
+++
+++



 6b
+++
++



 7a
+++
+++



 7b
+



 8a
+++
+++



 8b
+++
+



 9
++



10a
+++
+++



10b
++



11a
+++
+++



11b
++



11c
+++



11d
+



12a
+++
+++



12b
+++
+



12c
+++
++



12d
+++
+++



13a
+++
++



13b
+++
+++



13c and 13d
++



14a
++



14b
+++
+



15a
++



15b
++



15c
+++
+++



15d
+++
+++



16a
+++
+++



16b
+++
+++



17
+++
+++



18
++



19a
+++
+++



19b
+++
++



20a
+++
+++



20b
++



21a
+++
+++



21b
+++
++



22
+++
+++



23a
+++
+++



23b
+++
++



24a
+++
+++



24b
+++
+



25a
+++
+++



25b
++
+



26a
+++
+++



26b
+++
++



27
+++
++



28
+++
+++



29
+++
+++



30
++



31
+



32a
+++
+++



32b
+++
+







*+ means >1000 nM; ++ means 100 nM-1000 nM; +++ means <100 nM.






While we have described a number of embodiments of this invention, it is apparent that our basic examples may be altered to provide other embodiments that utilize the compounds and methods of this invention. Therefore, it will be appreciated that the scope of this invention is to be defined by the appended claims rather than by the specific embodiments that have been represented by way of example.
The contents of all references (including literature references, issued patents, published patent applications, and co-pending patent applications) cited throughout this application are hereby expressly incorporated herein in their entireties by reference. Unless otherwise defined, all technical and scientific terms used herein are accorded the meaning commonly known to one with ordinary skill in the art.

## Claims

1. A compound of the Formula:




or a pharmaceutically acceptable salt thereof, wherein
R2 is (C1-C3)alkyl;
R3 is hydrogen, monocyclic cycloalkyl, monocyclic heterocyclyl, or (C1-C6)alkyl, wherein the (C1-C6)alkyl is optionally substituted with 1 to 2 groups independently selected from hydroxy, halo, and cyano;
R4 is hydrogen, (C1-C3)alkyl, or ═O;
X is —C(O)NH— or —NHC(O)—;
m is 0, 1, or 2;
n is 0, 1, 2, or 3;
Cy1 is phenyl or pyridinyl, each of which is optionally substituted with 1 to 2 groups independently selected from R5;
Cy2 is cyclohexyl or phenyl, each of which is optionally substituted with 1 to 2 groups independently selected from R6;
R5 is (C1-C3)alkylsulfonyl;
R6 is selected from (C1-C3)alkyl, halo(C1-C3)alkyl, hydroxy, and (C1-C3)alkoxy; and
R7 and R8 are each independently hydrogen, hydroxy, (C1-C3)alkyl, hydroxy(C1-C3)alkyl, (C1-C3)alkoxy(C1-C3)alkyl, aminocarbonyl-O(C1-C3)alkyl, hydroxycarbonyl(C1-C3)alkoxy(C1-C3)alkyl, (C1-C3)alkylcarbonylamino(C1-C3)alkyl, (C1-C3)alkoxycarbonylamino(C1-C3)alkyl, di(C1-C3)alkylamino(C1-C3)alkyl, di(C1-C3)alkylaminocarbonyl(C1-C3)alkyl, (C1-C3)alkylaminocarbonyl(C1-C3)alkyl, aminocarbonyl(C1-C3)alkyl, aminocarbonyl, mono(C1-C3)alkylamino, di(C1-C3)alkylamino, CO2H, (CH2)1-3COOH, moncyclic heterocyclyl, (C1-C3)alkoxycarbonyl, halophenyl, halophenyl(C1-C3)alkyl, or quinolin-2(1H)one-4yl-methyl; or
R7 and R8, together with the carbon atom to which they are attached, form a 3- to 6-membered cycloalkyl or heterocyclyl.

2. The compound of claim 1, wherein the compound is of the Formula:



or a pharmaceutically acceptable salt thereof.

3. The compound of claim 2, wherein the compound is of the Formula:



or a pharmaceutically acceptable salt thereof.

4. The compound of claim 3, wherein R7 is hydrogen; and R8 is hydrogen, hydroxy(C1-C3)alkyl, (C1-C3)alkoxy(C1-C3)alkyl, aminocarbonyl-O(C1-C3)alkyl, hydroxycarbonyl(C1-C3)alkoxy(C1-C3)alkyl, (C1-C3)alkylcarbonylamino(C1-C3)alkyl, (C1-C3)alkoxycarbonylamino(C1-C3)alkyl, di(C1-C3)alkylamino(C1-C3)alkyl, di(C1-C3)alkylaminocarbonyl(C1-C3)alkyl, (C1-C3)alkylaminocarbonyl(C1-C3)alkyl, aminocarbonyl(C1-C3)alkyl, or aminocarbonyl.

5. The compound of claim 4, wherein R7 is hydrogen; and R8 is hydrogen, —CH2OH, —(CH2)2OH, —CH2OCH3, —CH2OC(O)NH2, —CH2OCH2COOH, —CH2NHC(O)CH3, —CH2NHC(O)OCH3, —(CH2)2N(CH3)2, —CH2C(O)N(CH3)2, —CH2C(O)NHCH3, —CH2C(O)NH2, or CONH2.

6. The compound of claim 5, wherein R7 is hydrogen; and R8 is hydrogen, —CH2OH, —(CH2)2OH, or —CH2OCH3.

7. The compound of claim 6, wherein R5 is —SO2CH2CH3 or —SO2CH3.

8. The compound of claim 7, wherein R6 is CF3.

9. The compound of claim 8, wherein R2 is ethyl or isopropyl.

10. A compound selected from:




























or a pharmaceutically acceptable salt thereof.

11. A pharmaceutical composition comprising a compound of claim 1, or a pharmaceutically acceptable salt thereof; and a pharmaceutically acceptable carrier.

12. A method of treating one or more diseases or disorders selected from asthma, chronic obstructive pulmonary disease (COPD), allergic rhinitis, atopic dermatitis, contact dermatitis, acne, urticaria, cystic fibrosis, allograft rejection, multiple sclerosis, scleroderma, arthritis, rheumatoid arthritis, juvenile rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, systemic lupus erythematosus (SLE), psoriasis, Hashimoto's disease, pancreatitis, autoimmune diabetes, type I diabetes, autoimmune ocular disease, ulcerative colitis, Crohn's disease, regional enteritis, inflammatory bowel disease (IBD), inflammatory bowel syndrome (IBS), Sjögren's syndrome, obesity, hepatosteatosis, adipose tissue-associated inflammation, insulin resistance, type II diabetes, neuromyelitis optica, myasthenia gravis, dry eye, uveitis, Guillain-Barré syndrome, psoriasis, psoriatic arthritis (PsA), steroid resistant asthma, Graves' disease, scleritis, major depression, seasonal affective disorder, bipolar disorder, autism, epilepsy, Alzheimer's, endometriosis, obstructive sleep apnea syndrome (OSAS), Behçet's disease, dermatomyositis, polymyocitis, graft versus host disease, primary biliary cirrhosis, liver fibrosis, non-alcoholic fatty liver disease (NAFLD), sarcoidosis, primary sclerosing cholangitis, autoimmune thyroid disease, autoimmune polyendocrine syndrome type I, autoimmune polyendocrine syndrome type II, celiac disease, neuromyelitis, juvenile idiopathic arthritis, systemic sclerosis, myocardial infarction, pulmonary hypertension, osteoarthritis, cutaneous leishmaniasis, sinonasal polyposis, and cancer in a subject, comprising administering to the subject a therapeutically effective amount of a compound according to claim 1, or a pharmaceutically acceptable salt thereof.