### Requested TIC Data Rick Wilburn to: tzick, Lambesis.Christopher, Ramaly.Todd 09/22/2010 09:51 AM Cc: "Gary Wood"

"Rick Wilburn" <wilburnr@trimatrixlabs.com > <tzick@cticompanies.com >, <Lambesis.Christopher@epamail.epa.gov >, <Ramaly.Todd@epamail.epa.gov > 'Gary Wood" <woodgl@trimatrixlabs.com > 

### Please respond to <wilbumr@trimatrixlabs .com>

Attached please find the requested TIC data. Hopefully this is what you are looking for.

Rick

Corporate / Laboratory 5560 Corporate Exchange Court SE Grand Rapids, Michigan 49512 wilburnr@trimatrixlabs.com Main:616-975-4500 Direct:616-940-4246 Fax:616-942-7463

## TIC Calculations

Example

Sample Peak Area Internal Standard Concentration x Final Extract Volume = ug/g = (mg / kg)

Internal S tan Bard 

### Peak Area Response Factor Initial Sample Volume

Sample #1003151-03

2212126 x 5 ug/mL 7.17 ug/mLx10mL =2.41ug /g =mg/kg 1542096 1 29.8g



### Sample #1003151-04

2341013 x 5ug/mL 6.28 ug/mLx10 

### mL _2.08u /g=mg/kg 1863146 1 30.2g

Sample #1003151-05
8455431 5ug/mL 16.0 ug/mLx10mL =5.35 ug/g =mg/kg 2641963 x 1 = 29.9gFile :C:\Documents and Settings\analystsv\Desktop\031710\1003151-0 3.D Operator : JLB
Instrument : 308 Acquired : 17 Mar 2010 16:00 using AcqMethod 030810BNA.M Sample Name: 1003151-03 Misc Info :File :C:\Documents and Settings\analystsv\Desktop\031710\1003151-0
3.D Operator : JLB
Instrument : 308 Acquired : 17 Mar 2010 16:00 using AcqMethod 030810BNA.M Sample Name: 1003151-03 Misc Info

### Tic: 1003151-03.D\data.ms 1003151-03

Peak # Ret Time Type width Area Start Time End Time 1 7.919 rm 0.111 1542096 7.901 8.012 2 8.117 rm 0.093 2212126 8.070 8.163File :C:\Documents and Settings\analystsv\Desktop\031710\1003151-0
4.D Operator : JLB
Instrument : 308 Acquired : 17 Mar 2010 16:35 using AcqMethod 030810BNA.M Sample Name: 1003151-04 Misc Info :File :C:\Documents and Settings\analystsv\Desktop\031710\1003151-0
4.0 Operator : JLB
Instrument : 308 Acquired : 17 Mar 2010 16:35 using AcqMethod 030810BNA.M Sample Name: 1003151-04 Misc Info :TIC: 1003151-04.D\data.ms 1003151-04

Peak # 

### Ret Time 

### Type Width Area Start Time End Time 1 7.936 rm 0.128 1863146 7.831 7.960 2 8.134 rm 0.070 2341013 8.099 8.169File :C:\Documents and Settings\analystsv\Desktop\031710\1003151-0
5.D operator : JLB
Instrument : 308 Acquired : 17 Mar 2010 17:10 using AcqMethod 030810BNA.M Sample Name: 1003151-05 Misc Info :File :C:\Documents and Settings\analystsv\Desktop\031710\1003151-0
5.D Operator : OMB
Instrument : 308 Acquired : 17 Mar 2010 17:10 using AcqMethod 030810BNA.M Sample Name: 1003151-05 Misc Info :TIC: 1003151-05.D\data.ms 1003151-05

Peak # Ret Time Type Width Area Start Time End Time 1 7.959 rm 0.198 2641963 7.854 8.053 2 8.128 rm 0.192 8455431 8.088 8.280

### LSC Area Percent Report

Data Path : C:\msdchem\l\DATA\031710\ 

### Data File : 1003151-04.D Acq On : 17 Mar 2010 16:35 Operator : JLB Sample : 1003151-04 Misc : ALS Vial : 35 Sample Multiplier: 1

Integration Parameters: lscint.p Integrator: RTE Smoothing : ON Filtering: 5 

### Sampling : 1 Min Area: 0 % of largest Peak

. Start Thrs: 0.001 Max Peaks: 100 Stop Thrs : 0 Peak Location: TOP

If leading or trailing edge < 

### 100 prefer < Baseline drop else tangent > Peak separation: 0

Method : C:\msdchem\1\METHODS\030910bna.M Title : 8270/625 BNA

Signal : TIC: 1003151-04.D\data.ms

peak R.T. first max last PK peak corr. corr. % of # min scan scan scan TY height area % max. 

### total

1 8.134' 899 906 913 rBV 703716 2495336 1.04% 1.029% 2 8.204 915 918 926 rVB7 117537 234033 0.10% 0.096% 3- 

### 8.286. 926 932 934 rBV3 91586 130076 0.05% 0.054 1 6. - 4 8.461 934 962 964 rBV 45991560 239685484 100.00% 98.821%

Sum of corrected areas: 242544929File :C:\Documents and Settings\analystsv\Desktop\031710\1003151-0
4.D Operator : JLB
Instrument : 308 Acquired : 17 Mar 2010 16:35 using AcqMethod 030810BNA.M Sample Name: 1003151-04 Misc Info :File :C:\Documents and Settings\analystsv\Desktop\031710\1003151-0
4.D Operator : JLB Instrument : 308 Acquired : 17 Mar 2010 16:35 using AcqMethod 030810BNA.M Sample Name: 1003151-04 Misc Info :File :C:\Documents and Settings\analystsv\Desktop\031710\1003151-0
4.D Operator : JLB
Instrument : 308 Acquired : 17 Mar 2010 16:35 using AcqMethod 030810BNA.M Sample Name: 1003151-04 Misc Info :File :C:\Documents and Settings\analystsv\Desktop\031710\1003151-0 4.D Operator : JLB
Instrument : 308 Acquired : 17 Mar 2010 16:35 using AcqMethod 030810BNA.M Sample Name:

. 1003151-04 Misc Info :TIC: 1003151-04.D\data.ms 1003151-04

Peak # Ret Time Type width Area Start Time End Time 1 7.936 rm 0.117 2103761 7.849 7.965 2 8.134 rm 0.076 2667682 8.099 8.175 3 8.204 rm 0.029 51180 8.187 8.216 4 8.286 rm 0.029 69154 8.268 8.298 5 8.461 rm 0.350 246735988 8.298 8.647LSC Area Percent Report

Data Path : C:\msdchem\i\DATA\031710\ Data File : 1003151-06.D Acq On : 17 Mar 2010 17:45 Operator : JLB Sample : 1003151-06 Misc ALS Vial : 38 Sample Multiplier: 1

Integration Parameters: lscint.p Integrator: RTE Smoothing : ON 

### Filtering: 5 Sampling : 1 Min Area: 0

. % of largest Peak Start Thrs: 0.001 Max Peaks: 100 Stop Thrs : 0 Peak Location: TOP

If leading or trailing edge < 100 prefer < Baseline drop else tangent > Peak separation: 0



### Method : C:\msdchem\1\METHOD3\030910bna.M Title : 8270/625 

### BNA

Signal : TIC: 1003151-06.D\data.ms

peak R.T. first max last PK peak corr. corr. % of 1 min scan scan scan TY height area % max. total

1 8.158 900 910 925 rBV3 1425347 6853824 100.00% 88.614% 2 8.257 925 927 939 rVB 253801 880649 12.85% 11.386%

Sum of corrected areas: 7734473File :C:\Documents and Settings\analystsv\Desktop\031710\1003151-0 6.D Operator : JLB
Instrument : 308 Acquired : 17 Mar 2010 17:45 using AcqMethod 030810BNA.M Sample Name: 1003151-06 Misc Info :File :C:\Documents and Settings\analystsv\Desktop\031710\1003151-0
6.D Operator : JLB
Instrument : 308 Acquired : 17 Mar 2010 17:45 using AcqMethod 030810BNA.M Sample Name: 1003151-06 Misc Info :TIC: 1003151-06.D\data.ms 1003151-06

'eak # Ret Time Type Width Area Start Time End Time 1 7.971 rm 0.210 3240592 7.890 8.099 2 8.158 rm 0.099 5986821 8.128 8.228 3 8.257 rm 0.029 57487 8.245 8.274