# Patent

## Title
Small-molecule inhibitors of the androgen receptor

## Abstract

The present invention provides a method of inhibiting an androgen receptor by administering a compound of Formula I:




or a compound of Formula II:





wherein R1, R2, R3 and R8 are each independently hydrogen or C1-6 alkyl. R4 is absent or is hydrogen, C1-6 alkyl or C1-6 alkyl-OH. R5 is hydrogen, C1-6 alkyl or —NR6R7. R6 and R7 are each independently hydrogen or C1-6 alkyl, or are combined with the nitrogen to which they are attached to form a heterocycloalkyl having from 5 to 7 ring members. L is a linker of C1-6 alkylene, C2-6 alkenylene, C2-6 alkynylene or C3-6 cycloalkylene. The compounds of Formula I include the salts, hydrates and prodrugs thereof. Each R9 is H, C1-6 alkyl, —OH or —O—C1-6 alkyl. The compounds of Formulas I and II include the salts, hydrates and prodrugs thereof. By administering the compound of Formula I or II, the method inhibits the androgen receptor.

## Background
CROSS-REFERENCES TO RELATED APPLICATIONS
The present application is a continuation of U.S. Ser. No. 12/101,680, filed Apr. 11, 2008, which is related to U.S. Ser. No. 60/911,578, filed Apr. 13, 2007, all of which are herein incorporated by reference in their entirety.


STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH AND DEVELOPMENT
Not Applicable
REFERENCE TO A “SEQUENCE LISTING,” A TABLE, OR A COMPUTER PROGRAM LISTING APPENDIX SUBMITTED ON A COMPACT DISK
Not Applicable
BACKGROUND OF THE INVENTION
Prostate cancer (PCa) is a leading cause of cancer morbidity and mortality in men, and the androgen receptor (AR) is the primary therapeutic target. In early PCa, anti-androgen therapy (AAT) is almost universally effective. This consists of one or more combinations of GnRH agonists (to suppress pituitary signaling), aromatase inhibitors (to decrease androgen production), and competitive AR antagonists (to block AR directly) such as hydroxy-flutamide (OH-F) or bicalutamide (BiC). This strategy usually works for several years, but over time tumor cells evolve mechanisms for continued growth under these conditions of androgen depletion. Most recurrent, or hormone-refractory prostate cancer (HRPC) is nonetheless dependent on AR-mediated signaling. This can include upregulation of AR protein expression levels, acquisition of mutations within AR that increase its activity in response to alternative hormones (including antagonists), or upregulation of co-activator proteins that augment AR activity. Thus, it is likely that new approaches to block AR activity could significantly extend or increase the effectiveness of AAT. This could consist of better competitive antagonists, and considerable efforts from pharmaceutical companies are already being brought to bear on this approach. For example, pyrvinium has shown efficacy against tumor cell proliferation in vitro and in mouse models (see WO2006/078754 and Cancer Cell 2006, 10, 321). This implies that novel anti-androgens might have considerable utility in the treatment of both primary and recurrent PCa. Such anti-androgens might not be competitive antagonists that directly bind AR, and could conceivably function via inhibition of downstream events in AR signaling. Accordingly, there is a need for novel, potent anti-androgens. Surprisingly, this invention meets this, and other, needs.
BRIEF SUMMARY OF THE INVENTION
In one embodiment, the present invention provides a compound of Formula I:




wherein R1, R2 and R3 are each independently hydrogen or C1-6 alkyl. R4 is absent or is hydrogen, C1-6 alkyl or C1-6 alkyl-OH. R5 is hydrogen, C1-6 alkyl or —NR6R7. R6 and R7 are each independently hydrogen or C1-6 alkyl, or are combined with the nitrogen to which they are attached to form a heterocycloalkyl having from 5 to 7 ring members. L is a linker of C1-6 alkylene, C2-6 alkenylene, C2-6 alkynylene or C3-6 cycloalkylene. The compounds of Formula I include the salts, hydrates and prodrugs thereof. With the proviso that when R1 is H, R2 and R3 are methyl, R5 is —N(Me)2, and L is ethenylene, R4 is other than methyl.

In a second embodiment, the present invention provides a pharmaceutical composition including a compound of the present invention and a pharmaceutically acceptable excipient.
In a third embodiment, the present invention provides a method of inhibiting an androgen receptor by administering to a patient in need of such treatment, a therapeutically effective amount of a compound of Formula I. Compounds useful in the methods of the present invention include compounds of Formula I:




wherein R1, R2 and R3 are each independently hydrogen or C1-6 alkyl. R4 is absent or is hydrogen, C1-6 alkyl or C1-6 alkyl-OH. R5 is hydrogen, C1-6 alkyl or —NR6R7. R6 and R7 are each independently hydrogen or C1-6 alkyl, or are combined with the nitrogen to which they are attached to form a heterocycloalkyl having from 5 to 7 ring members. L is a linker of C1-6 alkylene, C2-6 alkenylene, C2-6 alkynylene or C3-6 cycloalkylene. The compounds of Formula I include the salts, hydrates and prodrugs thereof. By administering the compound of Formula I, the method inhibits the androgen receptor.

In a fourth embodiment, the present invention provides a pharmaceutical composition comprising a therapeutically effective amount of a compound of the present invention, in combination with a therapeutically effective amount of an anti-androgen compound or a LnRH agonist compound.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1. Compound screening strategy. Novel inhibitors of AR conformation change were discovered by creating a HEK-293 (ATCC CRL-1573)-derived cell line stably expressing a CFP N-terminal and YFP C-terminal tagged AR vector that has been previously used to measure conformation change by FRET. C-AR-Y was stably expressed in either LAPC4 or HEK293 cell lines; in parallel, HEK293 cells were transfected with native AR along with MMTV-luciferase. Cells were cultured in the presence or absence of 10 nM DHT and one of 1040 FDA-approved drugs. The screen was performed in duplicate for each condition, and the top 50 compounds with activities in both trials were selected. Each of the top compounds was evaluated in detail with a dose-response study, and the “validated” compounds were compared across all assays. The validated hits accounted for approximately 40% of the hits initially identified for each screen.
FIG. 2. Analysis of hits. 1040 FDA-approved drugs were tested in three different assays, a transcription-based assay using HEK293 cells expressing MMTV-luciferase, conformation-based assays using HEK293/C-AR-Y stable cells, and LAPC4/C-AR-Y stable cells. Fewer compounds affected AR conformational change vs. transcriptional output. LAPC4 cells were the least sensitive to test compounds. High selectivity (<50 hits) was observed for the transcription assay when the cutoff was set between 5 and 6 standard deviations (SD) from the mean (as determined by multiple replicates of cells treated only with DHT). Similar selectivity was observed for the HEK293/C-AR-Y cells between 3 and 4 SD, whereas in the LAPC4/C-AR-Y cells such effects were observed between 2 and 3 SD from the mean. The transcriptional assay used in the screen had a Z-factor=0.6, vs. 0.6 for the LAPC4 FRET assay and 0.5 for the HEK293 FRET assay (Zhang, J. H., T. D. Chung, and K. R. Oldenburg, A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J Biomol Screen, 1999. 4(2): p. 67-73).
FIG. 3. Characterization of hits in primary screen. (A) Hits that scored in the primary assays were ranked according to efficacy based on the average of duplicate readings, and the top 50 from each assay was compared to the other two. In each case, a minority of compounds were shared between the assays, and most hits were unique to a particular system. (B) Validated hits were determined by detailed dose-response in the original assay used, and only compounds that exhibited pharmacologic effects were counted. These hits were then cross-compared to the other screening assays, using a dose-response. In this secondary analysis, the majority of hits from any one assay were also effective in another assay system.
FIG. 4. Pyrvinium pamoate (PP) exhibits identical responses vs. pyrvinium chloride (PCl). Anion exchange was used to replace the pamoate salt with a chloride ion. The resulting compound, PCl, had an identical dose-response vs. the parent compound in blocking PSA reporter activity in LAPC4 cells.
FIG. 5. PP and HH inhibit DHT-induced gene expression differently than a competitive antagonist. LNCaP cells transiently transfected with a PSA-luciferase reporter were exposed to OH-F, PP and HH. DHT was titrated. Whereas OH-F caused a modified DHT dose-response consistent with a competitive antagonist, PP and HH exhibited a pattern consistent with a non-competitive antagonist.
FIG. 6. PP and HH inhibit androgen-induced cell proliferation. PP, HH, and BiC were compared for their ability to inhibit androgen dependent and independent growth in several cultured cell lines: LNCaP, LAPC4, LN-AR (a line that exhibits androgen-independent growth), and HEK293 cells. PP and HH each exhibited growth-inhibitory properties in LNCaP cells, whereas HH was not effective in LAPC4 cells. Neither compound exhibited non-specific growth inhibition of HEK293 cells. Importantly, PP blocked growth of “hormone refractory” LN-AR cells. Asterisk (*)=p<0.005.
FIG. 7. PP synergistically reduces prostate size in mice. Cohorts of nine male mice were treated with PO gavage of BiC (100 mg/kg), IP injection of PP (1 mg/kg), or the combination for four weeks. As a positive control, nine mice were treated with castration for four weeks. Prostate glands were harvested and wet weights determined. PP alone did not significantly reduce prostate size. BiC treatment significantly reduced prostate weight by 35%, and the combination of PP:BiC reduced the weight by 63% (p<0.0005, t-test), implying a synergistic effect of PP. cntrl: untreated mice; BiC: bicalutamide; PP: pyrvinium pamoate; cast: castrated. Error bars represent the standard error of the mean (S.E.M.).
FIG. 8. PP suppresses androgen-dependent gene expression in the prostate, and augments BiC activity. Total RNA was extracted from prostate glands of cohorts of 9 mice used to test PP in vivo. qRT-PCR was performed to assess gene expression levels of five androgen-induced genes. Gene expression levels are expressed relative to RPL19, an androgen-unresponsive gene. PP significantly suppressed gene expression in all cases, and augmented the effects of BiC, with one exception (TMPRSS2), which may have been maximally suppressed by each treatment alone. cntrl: untreated; BiC: bicalutamide; PP: pyrvinium pamoate; cast: castrated.
FIG. 9. Inhibitors of the androgen receptor.
FIG. 10. Inhibitors of the androgen receptor.
FIG. 11. Synthesis of demethyl pyrvinium.
FIG. 12. Synthesis of naphthalene analog of pyrvinium.



DETAILED DESCRIPTION OF THE INVENTION
I. Introduction
The present invention provides a method of inhibiting an androgen receptor by administering to a patient in need of such treatment, a therapeutically effective amount of a compound of Formula I or Formula II:




The compounds of the present invention are believed to inhibit folding of the androgen receptor, thus inhibiting receptor activation. The compounds of the present invention can be used to treat any disease involving folding of the androgen receptor. Patients in need of such treatment often suffer from prostate cancer, including primary and hormone refractory prostate cancer, ovarian cancer, hepatocellular carcinoma, acne vulgaris, endometriosis, acanthosis nigricans, hypertrichosis, breast cancer, precocious puberty, polycystic ovary syndrome, benign prostatic hyperplasia, alopecia (such as androgen-dependent alopecia), hirsutism and hypersexuality/paraphilia.
The compounds of the present invention can be used to inhibit other nuclear receptors and treat associated disease states. Receptor activation of PPARγ can be inhibited using the compounds of the present invention, thereby treating disease states such as insulin resistance, diabetes and lipodystrophy, including cholesterol disorders. The compounds of the present invention are useful in treating disease states associated with estrogen receptor α and β, such as breast, colon, ovarian and endometrial cancers, as well as in metabolic regulation. Other disease states that can be treated with the compounds of the present invention include those associated with the thyroid hormone receptor, such as thyroid and cardiac disorders. Such compounds can also be used to augment the inhibition of the glucocorticoid receptor, which is used for immune suppression in a multitude of diseases. The compounds of the present invention can also inhibit the progesterone receptor, resulting in termination of a pregnancy
II. Definitions
As used herein, “administering” refers to oral administration, administration as a suppository, topical contact, parenteral, intravenous, intraperitoneal, intramuscular, intralesional, intranasal or subcutaneous administration, intrathecal administration, or the implantation of a slow-release device e.g., a mini-osmotic pump, to the subject.
As used herein, the term “alkyl” refers to a straight or branched, saturated, aliphatic radical having the number of carbon atoms indicated. For example, C1-C6 alkyl includes, but is not limited to, methyl, ethyl, propyl, butyl, pentyl, hexyl, iso-propyl, iso-butyl, sec-butyl, tert-butyl, etc.
As used herein, the term “alkylene” refers to an alkyl group linking at least two other groups, i.e. a divalent hydrocarbon radical of 1 to 6 carbon atoms. As for alkyl, the alkylene group can be straight or branched. For instance, a straight chain alkylene can be the bivalent radical of —(CH2)n′′, where n is 1, 2, 3, 4, 5 or 6. Alkylene groups include, but are not limited to, methylene, ethylene, propylene, butylene, pentylene and hexylene. Similarly, alkenylene, alkynylene and cycloalkylene are divalent radicals of alkenyl, alkynyl and cycloalkyl (see within).
As used herein, the term “heterocycloalkyl” refers to a ring system having from 3 ring members to about 20 ring members and from 1 to about 5 heteroatoms such as N, O and S. Additional heteroatoms can also be useful, including, but not limited to, B, Al, Si and P. The heteroatoms can also be oxidized, such as, but not limited to, —S(O)— and —S(O)2—. For example, heterocycle includes, but is not limited to, tetrahydrofuranyl, tetrahydrothiophenyl, morpholino, pyrrolidinyl, pyrrolinyl, imidazolidinyl, imidazolinyl, pyrazolidinyl, pyrazolinyl, piperazinyl, piperidinyl, indolinyl, quinuclidinyl and 1,4-dioxa-8-aza-spiro[4.5]dec-8-yl.
As used herein, the term “androgen receptor” refers to an intracellular steroid receptor of the nuclear receptor super-family that specifically binds androgens such as testosterone and dihydrotestosterone.
As used herein, the term “anti-androgen” refers to a group of hormone receptor antagonist compounds that are capable of preventing or inhibiting the biologic effects of androgens, male sex hormones, on normally responsive tissues in the body. Antiandrogens usually work by blocking the appropriate receptors, competing for binding sites on intracellular receptors, and obstructing androgen signaling pathways. As well as the compounds of Formula I and II, anti-androgens include, but are not limited to, coumarins, bicalutamide, flutamide, hydroxyflutamide, nilutamide, spionolactone, cyproterone acetate, ketoconazole, finasteride, dutasteride, harman, norharman, harmine, harmaline, tetrahydroharmine, harmol, harmalol, ethyl harmol, n-butyl harmol and other beta-carboline derivatives.
Antiandrogens are often indicated to treat severe male sexual disorders, such as hypersexuality (excessive sexual desire) and sexual deviation, specifically paraphilias, as well as use as an antineoplastic agent and palliative, adjuvant or neoadjuvant hormonal therapy in prostate cancer. Antiandrogens can also be used for treatment of benign prostatic hyperplasia (prostate enlargement), acne vulgaris, androgenetic alopecia (male pattern baldness), and hirsutism (excessive hair growth). Anti-androgens are also occasionally used as a male contraceptive agent, to purposefully prevent or counteract masculinisation in the case of transgender women undergoing gender reassignment therapy, and to prevent the symptoms associated with reduced testosterone, such as hot flashes, following castration. Other conditions treatable with an anti-androgen are prostate cancer, including primary and hormone refractory prostate cancer, ovarian cancer, hepatocellular carcinoma, acne vulgaris, endometriosis, acanthosis nigricans, hypertrichosis, breast cancer, precocious puberty, polycystic ovary syndrome, benign prostatic hyperplasia, alopecia (such as androgen-dependent alopecia), hirsutism and hypersexuality/paraphilia.
As used herein, the term “a combination of active agents” refers to a composition of at least two or more active agents.
As used herein, the term “counterion” refers to the ion that accompanies an ionic species in order to maintain electronic neutrality. Counterions can be atomic, such as fluoride, chloride, bromide, iodide, or metallic counterions. Counterions can also be molecular, such as acetate, succinate, maleate and embonate (pamoate). Counterions can be positively or negatively charged. Counterions of the present invention are negatively charged. In addition, counterions can have a charge greater than 1, such as 2 or more. One of skill in the art will appreciate that other counterions are useful in the present invention.
As used herein, the term “hormonal therapy” refers to the use of hormones in medical treatment, as well as the inhibition of hormone production, such as the use of direct competitors to hormones, such as antiandrogens.
As used herein, the term “hydrate” refers to a compound that is complexed to at least one water molecule. The compounds of the present invention can be complexed with from 1 to 10 water molecules.
As used herein, the term “inhibiting” refers to a compound that partially or fully prohibits or a method of partially or fully prohibiting a specific action or function.
As used herein, the term “LnRH agonist” refers to a compound or biological molecule that binds to the luteinizing releasing-hormone receptor.
As used herein, the term “patient in need” refers to a patient suffering from prostate cancer, polycystic ovary syndrome, benign prostatic hyperplasia, alopecia and hirsutism. Other conditions that a patient in need suffers from include, but are not limited to, ovarian cancer, hepatocellular carcinoma, acne vulgaris, endometriosis, acanthosis nigricans, hypertrichosis, breast cancer, precocious puberty and hypersexuality/paraphilia. Patients suffering from other conditions treatable with anti-androgens are also treatable with the methods of the present invention. Patients treatable using the methods of the present invention are animals such as mammals, including, but not limited to, primates (e.g., humans), cows, sheep, goats, horses, dogs, cats, rabbits, rats, mice and the like. In certain embodiments, the patient is a human.
As used herein, the term “prodrug” refers to covalently bonded carriers which are capable of releasing the active agent of the methods of the present invention, when the prodrug is administered to a mammalian subject. Release of the active ingredient occurs in vivo. Prodrugs can be prepared by techniques known to one skilled in the art. These techniques generally modify appropriate functional groups in a given compound. These modified functional groups however regenerate original functional groups by routine manipulation or in vivo. Prodrugs of the active agents of the present invention include active agents wherein a hydroxy, amidino, guanidino, amino, carboxylic or a similar group is modified.
As used herein, the term “salt” refers to acid or base salts of the compounds used in the methods of the present invention. Illustrative examples of pharmaceutically acceptable salts are mineral acid (hydrochloric acid, hydrobromic acid, phosphoric acid, and the like) salts, organic acid (acetic acid, propionic acid, glutamic acid, citric acid and the like) salts, quaternary ammonium (methyl iodide, ethyl iodide, and the like) salts. It is understood that the pharmaceutically acceptable salts are non-toxic. Additional information on suitable pharmaceutically acceptable salts can be found in Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, Pa., 1985, which is incorporated herein by reference.
Pharmaceutically acceptable salts of the basic compounds of the present invention are salts formed with acids, such as of mineral acids, organic carboxylic and organic sulfonic acids, e.g., hydrochloric acid, methanesulfonic acid, maleic acid, are also possible provided a basic group, such as pyridyl, constitutes part of the structure.
The neutral forms of the compounds may be regenerated by contacting the salt with a base or acid and isolating the parent compound in the conventional manner. The parent form of the compound differs from the various salt fowls in certain physical properties, such as solubility in polar solvents, but otherwise the salts are equivalent to the parent form of the compound for the purposes of the present invention.
As used herein, the terms “therapeutically effective amount or dose” or “therapeutically sufficient amount or dose” or “effective or sufficient amount or dose” refer to a dose that produces therapeutic effects for which it is administered. The exact dose will depend on the purpose of the treatment, and will be ascertainable by one skilled in the art using known techniques (see, e.g., Lieberman, Pharmaceutical Dosage Forms (vols. 1-3, 1992); Lloyd, The Art, Science and Technology of Pharmaceutical Compounding (1999); Pickar, Dosage Calculations (1999); and Remington: The Science and Practice of Pharmacy, 20th Edition, 2003, Gennaro, Ed., Lippincott, Williams & Wilkins). In sensitized cells, the therapeutically effective dose can often be lower than the conventional therapeutically effective dose for non-sensitized cells.
As used herein, the terms “treat”, “treating” and “treatment” refers to any indicia of success in the treatment or amelioration of an injury, pathology, condition, or symptom (e.g., pain), including any objective or subjective parameter such as abatement; remission; diminishing of symptoms or making the symptom, injury, pathology or condition more tolerable to the patient; decreasing the frequency or duration of the symptom or condition; or, in some situations, preventing the onset of the symptom or condition. The treatment or amelioration of symptoms can be based on any objective or subjective parameter; including, e.g., the result of a physical examination.
III. Method of Inhibiting an Androgen Receptor
The present invention provides a method of inhibiting an androgen receptor by administering to a patient in need of such treatment, a therapeutically effective amount of a compound of Formula I or Formula II, or compounds shown in FIG. 9 and FIG. 10. Compounds useful in the methods of the present invention include compounds of Formula I:




wherein R1, R2 and R3 are each independently hydrogen or C1-6 alkyl. R4 is absent or is hydrogen, C1-6 alkyl or C1-6 alkyl-OH. R5 is hydrogen, C1-6 alkyl or —NR6R7. R6 and R7 are each independently hydrogen or C1-6 alkyl, or are combined with the nitrogen to which they are attached to form a heterocycloalkyl having from 5 to 7 ring members. L is a linker of C1-6 alkylene, C2-6 alkenylene, C2-6 alkynylene or C3-6 cycloalkylene. The compounds of Formula I include the salts, hydrates and prodrugs thereof. By administering the compound of Formula I, the method inhibits the androgen receptor. Also useful in the methods of the present invention are compounds of Formula II:





In Formula II, R8 is hydrogen or C1-6 alkyl, and each R9 is H, C1-6 alkyl, —OH or —O—C1-6 alkyl. Salts, hydrates and prodrugs thereof, are also encompassed by Formula II.

Some of the compounds of Formulas I and II are already known. Other compounds of Formulas I and II are novel compounds. The compounds of Formulas I and II can be combined with other anti-androgen compounds such as coumarins, bicalutamide, flutamide, hydroxyflutamide, nilutamide, spionolactone, cyproterone acetate, ketoconazole, finasteride, dutasteride, harman, harmine, harmaline, tetrahydroharmine, harmol, harmalol, harmine acid, harmine acid methyl ester, harmilinic acid, harmanamide, acetylnorharmine, ethyl harmol, n-butyl harmol and other beta-carboline derivatives.
The compounds of the present invention are believed to inhibit folding of the androgen receptor, thus inhibiting receptor activation. Patients in need of such treatment often suffer from prostate cancer, including primary and hormone refractory prostate cancer, ovarian cancer, hepatocellular carcinoma, acne vulgaris, endometriosis, acanthosis nigricans, hypertrichosis, breast cancer, precocious puberty, polycystic ovary syndrome, benign prostatic hyperplasia, alopecia (such as androgen-dependent alopecia), hirsutism and hypersexuality/paraphilia. Other disease states can be treated using the methods of the present invention.
In another embodiment, the method of the present invention treats alopecia by topical administration of a compound or composition of the present invention.
In other embodiments, the compound of Formula I is administered with a course of hormonal therapy, where the compound for hormonal therapy is an anti-androgen or a LnRH agonist. In some embodiments, the compounds are administered separately. In other embodiments, the compounds are admixed. In some other embodiments, the compounds are administered at the same time. In still other embodiments, the compounds are administered at different times.
In some other embodiments, the compound of Formula I is administered in combination with a therapeutically effective amount of bicalutamide, flutamide, hydroxyflutamide, nilutamide, spionolactone, cyproterone acetate, ketoconazole, finasteride or dutasteride. In other embodiments, the compound of Formula I is administered in combination with a therapeutically effective amound of a coumarin.
In some embodiments, the method of the present invention does not include the use of pyrvinium to treat prostate cancer.
IV. Compounds for Inhibiting an Androgen Receptor
Compounds useful in the present invention are those that inhibit an androgen receptor. Useful compounds can be identified using the assay methods described in the Examples below. These compounds are commercially available or can be synthesized using methods known to those skilled in the art.
In some embodiments, compounds useful in the methods of the present invention include compounds of Formula I:




wherein R1, R2 and R3 are each independently hydrogen or C1-6 alkyl. R4 is absent or is hydrogen, C1-6 alkyl or C1-6 alkyl-OH. R5 is hydrogen, C1-6 alkyl or —NR6R7. R6 and R7 are each independently hydrogen or C1-6 alkyl, or are combined with the nitrogen to which they are attached to form a heterocycloalkyl having from 5 to 7 ring members. L is a linker of C1-6 alkylene, C2-6 alkenylene, C2-6 alkynylene or C3-6 cycloalkylene. The compounds of Formula I include the salts, hydrates and prodrugs thereof. With the proviso that when R1 is H, R2 and R3 are methyl, R5 is —N(Me)2, and L is ethenylene, R4 is other than methyl.

In some embodiments, L is ethylene, ethenylene or cyclopropylene. In other embodiments, R6 and R7 are each independently hydrogen or C1-6 alkyl, and L is a C2-6 alkenylene.
In some other embodiments, compounds useful in the methods of the present invention include compounds of Formula Ia:




wherein R4 is hydrogen, C1-6 alkyl or C1-6 alkyl-OH. In other embodiments, R4 is C1-6 alkyl-OH. In another embodiment, the compound of Formula Ia is:





In still other embodiments, the compound is a compound of Formula Ia where R4 is C1-6 alkyl. In yet other embodiments, the compounds of Formula Ia include:





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In still yet other embodiments, the compound of Formula Ia is:





In a further embodiment, the compound of Formula Ia is:





In other embodiments, the compounds of Formula I do not include pyrvinium:




In another embodiment, compounds useful of the present invention include compounds of Formula Ib:




In some other embodiments, the compound is:





In some embodiments, compounds useful in the methods of the present invention are provided by Formula Ic:




wherein R1, R2, R3, R4 and R5 are as defined above. The dashed line in Formula Ic represents an optional double bond. When the dashed line to the nitrogen is a bond, then the nitrogen is quaternary and has a positive charge. When the dashed line is absent, then the nitrogen is tertiary and neutral. In other embodiments, the compound has the formula:





In other embodiments, salt forms of the compounds of Formula I include a counterion of pamoate, chloride, bromide, succinate, maleate or acetate.
In some other embodiments, compounds useful in the methods of the present invention include compounds of Formula II:




In Formula II, R8 is hydrogen or C1-6 alkyl, and each R9 is H, C1-6 alkyl, —OH or —O—C1-6 alkyl. In some embodiments, each R9 can also be a carboxylic acid, an ester, an amide or a ketone. Salts, hydrates and prodrugs thereof, are also encompassed by Formula II.

The dashed lines of Formula II represent optional double bonds. When the dashed line to the nitrogen is a bond, then the nitrogen has no substituent. When the dashed line to the nitrogen is absent, then the nitrogen can be substituted with H or C1-6 alkyl. For example, the following structures are all encompassed by Formula II:




Each R9 group can be on any ring of the compound of Formula II. For example, the following structures are all encompassed by Formula II:





One of skill in the art will appreciate that other arrangements of the R2 substituents are possible on the compounds of Formula II.

Salts of the compounds of Formula II can be mineral acid (hydrochloric acid, hydrobromic acid, phosphoric acid, and the like) salts, organic acid (acetic acid, propionic acid, glutamic acid, citric acid, succinic acid, maleic acid and the like) salts. It is understood that the pharmaceutically acceptable salts are non-toxic. Additional information on suitable pharmaceutically acceptable salts can be found in Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, Pa., 1985, which is incorporated herein by reference.
Compounds of Formula II, include, but are not limited to:




In some embodiments, the compound of Formula II has the following formula:




Additional compounds useful in the methods of the present invention are coumarins, bicalutamide, flutamide, hydroxyflutamide, nilutamide, spionolactone, cyproterone acetate, ketoconazole, finasteride and dutasteride. Further compounds useful in the methods of the present invention are harman, harmine, harmaline, tetrahydroharmine, harmol, harmalol, harmine acid, harmine acid methyl ester, harmilinic acid, harmanamide, acetylnorharmine, ethyl harmol, n-butyl harmol and other beta-carboline derivatives, such as those shown below in Formula IIa:




wherein R′ is H or C1-6 alkyl. R′′ is H, C1-6 alkyl, —OR′′′ and —NR′′′2, wherein each R′′′ is independently H or C1-6 alkyl. One of skill in the art will appreciate that other androgen receptor inhibitors are useful in the methods of the present invention.

V. Formulations for Inhibiting an Androgen Receptor
The compounds of the present invention can be formulated in a variety of different manners known to one of skill in the art. Pharmaceutically acceptable excipients are determined in part by the particular composition being administered, as well as by the particular method used to administer the composition. Accordingly, there are a wide variety of suitable formulations of pharmaceutical compositions of the present invention (see, e.g., Remington's Pharmaceutical Sciences, 20th ed., 2003, supra). For example, the compounds of the present invention can be prepared and administered in a wide variety of oral, injectible and topical dosage forms. The compounds of the present invention can also be prepared and administered in parenteral dosage forms. Thus, the compounds of the present invention can be administered by injection, that is, intravenously, intramuscularly, intracutaneously, subcutaneously, intraduodenally, or intraperitoneally. Also, the compounds described herein can be administered by inhalation, for example, intranasally. Additionally, the compounds of the present invention can be administered transdermally or topically, e.g., in a liquid or gel form or as a patch.
Accordingly, the present invention also provides pharmaceutical compositions comprising a pharmaceutically acceptable carrier or excipient and one or more compounds of the invention. In some embodiments, the compound can be any of the following:




Formulations suitable for administration can consist of (a) liquid solutions, such as an effective amount of a compound of the present invention suspended in diluents, such as water, saline or PEG 400; (b) capsules, sachets, depots or tablets, each containing a predetermined amount of the active ingredient, as liquids, solids, granules or gelatin; (c) suspensions in an appropriate liquid; (d) suitable emulsions; and (e) patches. The pharmaceutical forms can include one or more of lactose, sucrose, mannitol, sorbitol, calcium phosphates, corn starch, potato starch, microcrystalline cellulose, gelatin, colloidal silicon dioxide, talc, magnesium stearate, stearic acid, and other excipients, colorants, fillers, binders, diluents, buffering agents, moistening agents, preservatives, flavoring agents, dyes, disintegrating agents, and pharmaceutically compatible carriers. Lozenge forms can comprise the active ingredient in a flavor, e.g., sucrose, as well as pastilles comprising the active ingredient in an inert base, such as gelatin and glycerin or sucrose and acacia emulsions, gels, and the like containing, in addition to the active ingredient, carriers known in the art.
The pharmaceutical preparation is preferably in unit dosage form. In such form the preparation is subdivided into unit doses containing appropriate quantities of the active component. The unit dosage form can be a packaged preparation, the package containing discrete quantities of preparation, such as packeted tablets, capsules, and powders in vials or ampoules. Also, the unit dosage form can be a capsule, tablet, cachet, or lozenge itself, or it can be the appropriate number of any of these in packaged form. The composition can, if desired, also contain other compatible therapeutic agents. Preferred pharmaceutical preparations can deliver the compounds of the invention in a sustained release formulation.
The pharmaceutical preparations are typically delivered to a mammal, including humans and non-human mammals. Non-human mammals treated using the present methods include domesticated animals (i.e., canine, feline, murine, rodentia, and lagomorpha) and agricultural animals (bovine, equine, ovine, porcine).
For cancer therapy, formulations of the present invention can include a compound of Formula I or II in combination with a therapeutically effective amount of an anti-androgen or an LnRH agonist. Anti-androgens, as described above, include, but are not limited to, coumarins, bicalutamide, flutamide, hydroxyflutamide, nilutamide, spionolactone, cyproterone acetate, ketoconazole, finasteride, dutasteride, harman, norharman, harmine, harmaline, tetrahydroharmine, harmol, harmalol, ethyl harmol, n-butyl harmol and other beta-carboline derivatives.
In practicing the methods of the present invention, the pharmaceutical compositions can be used alone, or in combination with other therapeutic or diagnostic agents. The additional anticancer drugs used in the combination protocols of the present invention can be formulated separately, or one or more of the anticancer drugs used in the combination protocols can be formulated together, such as in an admixture.
Formulations of the present invention can also include combinations of a compound of Formula I and a compound of Formula II. Additional therapeutic agents or diagnostic agents, such as those provided above, can also be formulated in combination with the combination of compounds of Formulas I and II.
VI. Administration to Inhibit an Androgen Receptor
The compounds of the present invention can be administered as frequently as necessary, including hourly, daily, weekly or monthly. The compounds utilized in the pharmaceutical method of the invention are administered at the initial dosage of about 0.0001 mg/kg to about 1000 mg/kg daily. A daily dose range of about 0.01 mg/kg to about 500 mg/kg, or about 0.1 mg/kg to about 200 mg/kg, or about 1 mg/kg to about 100 mg/kg, or about 10 mg/kg to about 50 mg/kg, can be used. The dosages, however, may be varied depending upon the requirements of the patient, the severity of the condition being treated, and the compound being employed. For example, dosages can be empirically determined considering the type and stage of disease diagnosed in a particular patient. The dose administered to a patient, in the context of the present invention should be sufficient to effect a beneficial therapeutic response in the patient over time. The size of the dose also will be determined by the existence, nature, and extent of any adverse side-effects that accompany the administration of a particular compound in a particular patient. Determination of the proper dosage for a particular situation is within the skill of the practitioner. Generally, treatment is initiated with smaller dosages which are less than the optimum dose of the compound. Thereafter, the dosage is increased by small increments until the optimum effect under circumstances is reached. For convenience, the total daily dosage may be divided and administered in portions during the day, if desired. Doses can be given daily, or on alternate days, as determined by the treating physician. Doses can also be given on a regular or continuous basis over longer periods of time (weeks, months or years), such as through the use of a subdermal capsule, sachet or depot, or via a patch.
The pharmaceutical compositions can be administered to the patient in a variety of ways, including topically, parenterally, intravenously, intradermally, intramuscularly, colonically, rectally or intraperitoneally. Preferably, the pharmaceutical compositions are administered parenterally, topically, intravenously, intramuscularly or orally.
In practicing the methods of the present invention, the pharmaceutical compositions can be used alone, or in combination with other therapeutic or diagnostic agents. The additional anticancer drugs used in the combination protocols of the present invention can be administered separately or one or more of the anticancer drugs used in the combination protocols can be administered together, such as in an admixture. Where one or more anticancer drug is administered separately, the timing and schedule of administration of each drug can vary. The other therapeutic or diagnostic agents can be administered at the same time as the compounds of Formulas I or II, separately or at different times.
The compounds of Formula I can also be administered in combination with the compound of Formula II. Additional therapeutic agents or diagnostic agents can be administered in combination with the combination of Formulas I and II.
The compounds of the present invention can be administered with a course of hormonal therapy. The compound for hormonal therapy includes, but is not limited to, an anti-androgen and a LnRH agonist.
In clinical studies, number of lesions, tumor size, and tumor growth rate can be monitored by radiography, tomography, and, where possible, direct measurement of tumor mass. Anti-tumor effects can also be measured using molecular biology and biochemistry techniques, such as ELISA, PCR, western blotting, or immunocytochemistry.
The pharmaceutically effective amount of a composition required as a dose will depend on the route of administration, the type of cancer being treated, and the physical characteristics of the patient. The dose can be tailored to achieve a desired effect, but will depend on such factors as body surface area, weight, diet, concurrent medication and other factors which those skilled in the medical arts will recognize.
The foregoing are general guidelines only that can be expanded or altered based on, for example, disease type and grade, patient age, health status, and sex, the particular drugs used in combination, the route and frequency of administration, and experimental and clinical findings using a multidrug combination.
VII. Examples
Compounds useful in the methods of the present invention can be prepared following the procedures set forth below. Pyrvinium pamoate is commercially available from MP Biochemicals (Solon, Ohio). Harmol HCl is commercially available from Sigma (St. Louis, Mo.).
Example 1
Preparation of Demethyl Pyrvinium
This example provides a synthetic procedure for demethyl pyrvinium.
LC-MS was performed using LC-MS (ODS-3, 5 um, columns 2.1×50 mm) with a mobile phase of 0.1% TFA aqueous solution and acetonitrile gradiently eluted.
Preparation of 2-Methyl-6-nitroquinoline (1)




4-Nitroaniline (6 g, 38 mmol) was dissolved in HCl (6N, 200 mL), the mixture was heated at 100° C. A solution of (E)-but-2-enal (7.2 mL, 76 mmol) in toluene (60 mL) was added dropwise, and the reaction was heated at 100° C. overnight. The reaction mixture was cooled to room temperature and basified with aqueous KOH until pH=12. The mixture was extracted with EtOAc and the organic layer was washed with brine and dried over anhydrous Na2SO4. 2-Methyl-6-nitroquinoline was obtained after purification by column chromatography (hexane:EtOAc=20:1) (5.8 g, 81.2%). MS [M++1]=189, LC-MS: tR=1.64 min.
Preparation of 2-Methylquinolin-6-amine (2)




To a solution of 2-methyl-6-nitroquinoline (12 g, 63.84 mmol) in HCl (1N, 450 mL) was added a solution of SnCl2.2H2O (72 g, 0.32 mol) in HCl (1N, 150 mL) slowly. The reaction mixture was heated to reflux for 15 min, then cooled to room temperature. The precipitate was filtered. The filtrate was basified by aqueous KOH and extracted with EtOAc. The organic layer was dried over anhydrous Na2SO4 and the solvent was removed under reduced pressure to give 6.9 g of 2-methylquinolin-6-amine (68.3%). MS[M++1]=159, LC-MS: tR=0.255 min.
Preparation of N,N-2-trimethylquinolin-6-amine (3)




A mixture of 2-methylquinolin-6-amine (2.4 g, 15.2 mmol) and NaH (2.4 g, 60.8 mmol) in DMF (60 mL) was stirred at 40° C. for 3 hrs. Then CH3I (3.8 mL, 60.8 mmol) was added by syringe. The reaction mixture was stirred at 40° C. overnight and then cooled to room temperature. The reaction mixture was diluted with water and extracted with EtOAc. The organic layer was washed with water and brine and dried over anhydrous Na2SO4. After purified by column chromatography (hexane/EtOAc=25:1), compound 3 was obtained (1.1 g, 39%). MS [M++1]=187, LC-MS: tR=0.775 min.
Preparation of 2,5-Dimethyl-1-phenyl-1H-pyrrole (4)




A 25 mL round-bottom flask was charged with aniline (1 mL, 8.77 mmol), hexane-2,5-dione (4 mL, 43.86 mmol) and AcOH (5 mL). The reaction mixture was refluxed for 4 hrs. The mixture was diluted with HCl (10%) and extracted with EtOAc. The organic layer was dried over Na2SO4 and the solvent was removed under reduced pressure to give 190 mg of compound 4 (75%). MS [M++1]=172, LC-MS: tR=2.54 min.
Preparation of 2,5-Dimethyl-1-phenyl-1H-pyrrole-3-carbaldehyde (5)




To a well stirred solution of 2,5-dimethyl-1-phenyl-1H-pyrrole (7.65 g, 44.7 mmol) and DMF (3.6 g, 49.2 mmol) in 70 mL of dry toluene, phosphorus oxychloride (6.9 g, 44.7 mmol) was added in small portions. The mixture was heated to 80° C. for 3 hrs. The solution was allowed to cool to room temperature, then 100 mL of saturated aqueous Na2CO3 was added. The aqueous layer was extracted with EtOAc (100 mL×3). The organic layer was dried over anhydrous Na2SO4 and the solvent was removed under reduced pressure, the residue was purified by column chromatography (hexane:EtOAc=10:1) to give 2.6 g of 2,5-dimethyl-1-phenyl-1H-pyrrole-3-carbaldehyde (29%). MS [M++1]=200, LC-MS: tR=1.96 min.
Preparation of N,N-Dimethyl-2-((E)-2-(2,5-dimethyl-1-phenyl-1H-pyrrol-3-yl)vinyl)quinolin-6-amine (6)




To a solution of compound 3 (316 mg, 1.7 mmol) and compound 5 (507 mg, 2.55 mmol) in DMF (25 mL) was added ZnCl2 (232 mg, 1.7 mmol). The reaction mixture was stirred at 160° C. overnight. Then the reaction mixture was diluted with aqueous NaOH (10%) and extracted with EtOAc. The organic layer was dried over anhydrous Na2SO4 and the solvent was removed under reduced pressure, the residue was purified by column chromatography (hexane:EtOAc=20:1) to give 13 mg of the target product (2.1%). MS[M++1]=368, LC-MS: tR=1.909 min
Example 2
Preparation of Naphthalene Analog of Pyrvinium
This example provides a synthetic procedure for the naphthalene analog of pyrvinium.
LCMS analysis followed the conditions described in Example 1.
Preparation of dibromomethyltriphenylphosphonium (7)
A solution of triphenylphosphine (10 g, 38 mmol) and dibromomethane (14.9 g, 85.3 mmol) in 80 mL of toluene was refluxed for 15 hrs. A white solid formed, the reaction mixture was filtered, the filter cake was washed with hexane (50 mL×3) to give 8.0 g of product as a white solid (Purity: 90%, yield: 48%).
Preparation of 2,5-Dimethyl-1-phenyl-1H-pyrrole-3-carbaldehyde (5)
Compound 5 was prepared as described in Example 1.
Preparation of 2,5-Dimethyl-1-phenyl-3-vinyl-1H-pyrrole (8)




To a solution of bromomethyl(triphenyl)phosphonium bromide (2.2 g, 5 mmol) in dry THF (50 mL), n-BuLi (2.5 M, 2 mL, 5 mmol) was added dropwise at −78° C. under nitrogen. The reaction mixture was stirred 30 min at this temperature and compound 5 (1.0 g, 5 mmol) was added, then the cooling bath was removed. Stirring was continued for another hour. The solvent was completely removed under reduced pressure. The residue and Pd(OAc)2 (56 mg, 0.25 mmol), Na2CO3 (1.86 g, 17.5 mmol), n-Bu4NCl (8.34 g, 30 mmol) were dissolved in dried DMF (20 mL). The reaction mixture was stirred at 100° C. for 3 hrs. The reaction was quenched by adding 20 mL of water, and the aqueous phase was extracted with EtOAc (30 mL×3). The combined extracts were dried over Na2SO4, the mixture was filtered and the filtrate was concentrated in vacuo to give the desired product as a colorless oil (Purity: 90%, yield: 80%). MS[M++1]=198, LC-MS: tR=2.76 min.
Preparation of 6-Bromonaphthalen-2-amine (9)




6-Bromo-2-naphthol (2.0 g, 9 mmol), ammonium sulfite (10.4 g, 90 mmol) and 10 mL of ammonia solution were sealed in a tube. The reaction mixture was heated at 190° C. for 24 hrs and allowed to cool to room temperature, the reaction mixture was extracted with EtOAc. HCl (10%) was added to the organic layer to give a precipitate. After filtration, the solid was dissolved in 10% of Na2CO3 and extracted with EtOAc, the organic layer was dried over Na2SO4. The solvent was removed under reduced pressure to give the product as yellow solid (0.67 g, 33%). MS[M++43]=265, LC-MS: tR=2.14 min.
Preparation of 6-Bromo-N,N-dimethylnaphthalen-2-amine (10)




A 100 mL oven dried round bottomed flask was charged with 6-bromo naphthalene-2-amine (0.6 g, 2.7 mmol) and NaH (60%, 0.43 g, 10.8 mmol). 10 mL of dry DMF was added under nitrogen atmosphere at room temperature and stirred for 1 hr, then CH3I (1.53 g, 10.8 mmol) was added by syringe. The reaction mixture was stirred at room temperature for 3 hrs. The reaction mixture was diluted with water (30 mL) and extracted with EtOAc. The organic layer was washed with water and brine and dried over anhydrous Na2SO4. After purified by column chromatography (hexane/EtOAc=100:1), compound 6 was obtained as yellow solid (0.35 g, 52%). MS[M+]=250, [M++43]=291, LC-MS: tR=2.81 min.
Preparation of N,N-Dimethyl-6-((E)-2-(2,5-dimethyl-1-phenyl-1H-pyrrol-3-yl)vinyl)naphthalen-2-amine (11)




Dry DMF (10 mL) was added by syringe to the mixture of 6-bromo-N,N-dimethylnaphthalen-2-amine (200 mg, 0.8 mmol), 2,5-dimethyl1-1-phenyl-3-vinyl-1H-pyrrole (236 mg, 1.2 mmol), Na2CO3 (254 mg, 2.4 mmol), n-Bu4NCl (224 mg, 0.8 mmol) and Pd(OAc)2 (8 mg, 0.33 mmol). The reaction mixture was stirred at 100 C for 3 hrs, then quenched with water (20 mL) and extracted with EtOAc. The organic layer was washed with brine and dried over anhydrous Na2SO4. After purified by column chromatography (hexane/EtOAc=500:1), the target compound was obtained as brown yellow solid (50 mg, 17%). MS[M+]=366, LC-MS: tR=3.28 min.
Example 3
Screening for Novel AR Antagonists
The assay below provides a method of identifying compounds that are androgen-receptor antagonists.
A library assembled by the NINDS was screened. The library consisted of 1040 FDA-approved drugs and natural products. A basic strategy to select and compare compounds from each of the primary screens was established (FIG. 1).
The transcription assay was conducted by transfecting 10 cm plates of HEK293 cells with plasmids encoding MMTV-luciferase, SV40-renilla luciferase, and native AR. These cells are useful because the limited transfection efficiency of prostate-derived cells increases the variability of the assay. The FRET assays were conducted using two independent cell lines, each stably expressing a CFP-AR-YFP fusion protein: HEK293/C-AR-Y and LAPC4/C-AR-Y. LAPC4 cells are a prostate derived line (Klein, K. A., et al., Progression of metastatic human prostate cancer to androgen independence in immunodeficient SCID mice. Nat Med, 1997. 3(4): p. 402-8). FRET is fluorescence resonance energy transfer between cyan and yellow fluorescent protein derivatives (CFP, YFP) that are fused to the amino and carboxyl termini of the human androgen receptor. When ligand activates the receptor, a conformational change takes place that brings the CFP and YFP into close proximity, thereby allowing FRET to occur. This spectroscopic change is detected using a fluorescence plate reader.
For the transcription assay, transfected cells were pooled before being plated into 96-well dishes with the test compounds at 10 μM. On each plate was included “no DHT” and “no treatment” controls, as well as a positive control with 1 μM OH-F. For the FRET assays, cells were directly plated into the 96-well plates with the test compounds at 10 μM. Cell and compound transfers were accomplished with a liquid handling robot. Cells were cultured for 24 hours in the presence of 10 nM DHT and the test compounds. After 24 hours, cells were either lysed by freeze-thaw (transcription assay), or fixed in 4% paraformaldehyde for reading on the FPR (FRET assays). Each compound was tested in duplicate in the initial screen.
The compounds were sorted on the basis of anti-androgen effects. In order to be selected for further analysis, a test compound had to function in both of the replicates, and in each case had to reduce the signal by at least one standard deviation from the mean of samples treated with DHT alone. This eliminated situations in which one test gave a strong response, and the replicate had none. Lastly, there must have been no evidence of cell toxicity, for the transcription assays specifically tested for renilla luciferase (which is discriminated from firefly luciferase on the basis of substrate specificity), and compounds that reduced this signal below two standard deviations were eliminated. For the FRET assays, toxic compounds were easily detected because of their effect on raw CFP and YFP signal, and compounds that reduced this signal below two standard deviations were eliminated.
Analysis of hits from the primary screen. FIG. 2 illustrates the results of the screen. The data clearly indicate that each assay system was capable of sorting compounds according to efficacy. Hits were sorted using different stringencies to determine the degree of variance from the mean that would be required to select a limited number of hits for each assay. The conformational assay, when used in either HEK293 or LAPC4 cells, achieved high selectivity using a lower stringency, whereas the transcription-based assay required higher degrees of stringency to achieve the same selectivity. Accordingly, conformational assays are less sensitive to non-specific cellular perturbation, whereas many compounds were capable of perturbing the transcriptional response.
The top 50 compounds identified in each primary assay were then tested in the other two assays. Among the top hits in each assay that were not included for further analysis were all known anti-androgens from the library (hydroxy-flutamide, nilutamide, cyproterone, and cyproterone acetate), and certain steroid hormones that exhibit cross-reactivity with AR at micromolar concentrations. A Venn diagram illustrates that there was modest overlap between the primary assays: approximately 20-30% of hits that scored in one assay also scored in another assay (FIG. 3).
To determine “validated” hits, the top 50 compounds were re-tested with titration studies in the original assays in which they were detected, and selected only those that exhibited a classic dose-response pattern. When validated hits from each assay were compared for activity in the others, a much higher proportion of compounds (approximately 55-82%) scored positive in at least one other assay than in the primary screen. Few compounds (3) scored positive in all three assays. This is consistent with the idea that each screening approach has the potential to identify compounds with efficacy across assays, but that it also might add a new dimension to drug discovery. Importantly, among the small number of validated hits, it was discovered that one compound in the HEK293 FRET assay, and three compounds in the LAPC4 FRET assay that initially did not meet selection criteria in the HEK293 primary transcription assay, but which showed inhibition of AR-mediated transcription upon subsequent careful analysis. Accordingly, the FRET assays can augment drug discovery vs. transcription assays alone.
Identification of novel anti-androgens. Several lead compounds survived the stringent screening protocol, and three classes with previously unrecognized anti-androgen activity: the coumarins, including warfarin, scopoletin, and esculin; harmol hydrochloride, a natural product (HH); and an FDA-approved anti-helminthic, pyrvinium pamoate (PP).
To evaluate the efficacy of pyrvinium and harmol against endogenous AR activity, each compound was tested in a dose-response assay in two prostate cancer-derived cell lines, LAPC4 (Klein, K. A., et al., Progression of metastatic human prostate cancer to androgen independence in immunodeficient SCID mice. Nat Med, 1997. 3(4): p. 402-8), and LNCaP (Horoszewicz, J. S., et al., The LNCaP cell line—a new model for studies on human prostatic carcinoma. Prog Clin Biol Res, 1980. 37: p. 115-32) that each express endogenous AR. LAPC4 cells express wild-type AR, whereas LNCaP cells, which are derived from hormone-refractory PCa, express AR with a mutation (T877A) that renders it responsive to a variety of ligands, including the antagonist hydroxy-flutamide (OH-F). Cells were transfected with two reporter plasmids, a PSA-luciferase construct (androgen responsive), and an SV40-renilla luciferase construct as an internal control. The following day, the cells were split and drugs added in the presence of 3 nM DHT. Luciferase production was measured the following day (Dual luciferase assay kit, Promega).
No compounds had androgen activity in the absence of DHT in either the conformation or the transcription reporter assays, even at doses exceeding 10 μM. PP and HH were much more effective than the competitive antagonists OH-F and bicalutamide (BiC) at inhibiting reporter transcription (Table 1), so the anti-androgen characteristics of these compounds were studied further. Both PP and HH inhibited DHT-responsive gene expression with efficacies far superior to BiC (Table 1). To rule out the possibility that the pamoate salt of pyrvinium was mediating transcriptional inhibition, anion exchange (Dowex) was used to replace the pamoate with a chloride ion. Pyrvinium chloride was just as effective as pyrvinium pamoate (FIG. 4).







TABLE 1







IC50 of various compounds identified in screens for


novel anti-androgens.












Activity on
Activity on




PSA-luciferase
PSA-luciferase




IC50(nM)
IC50(nM)



Compound
LNCaP
LAPC4















PP
24
12



HH
127
106



OH-F
agonist
130



Casodex
585
1,571



Esculin
62,159
831



Scopoletin
603,586
1,378



Warfarin
4,500
1,954



Harmine
1,560
80



Harmaline
1,190
nd



Harman
380
nd



Norharman
2,500
nd







PP = pyrvinium pamoate;



HH = harmol hydrochloride;



OH-F = hydroxy-flutamide;



BiC = bicalutamide.



nd = no data.






Example 4
Assay for Inhibition of an Androgen Receptor
The assay below demonstrates that the compounds identified in Example 2 inhibit endogenous androgen receptor gene expression and are not competitive antagonists.
To confirm that the effects of the lead compounds hold true for endogenous AR-regulated gene expression, quantitative RT-PCR (qRT-PCR) was used to monitor effects on a select number of androgen-responsive genes in LAPC4 and LNCaP cells. LAPC4 and LNCaP cells were grown in the presence or absence of 3 nM DHT in charcoal-stripped media, with or without the inhibitors. RNA was harvested using an RNAeasy kit (Qiagen) and reverse transcribed (MMLV-RT, Invitrogen). Real-time PCR was carried out on a 7300 Real Time PCR System (Applied Biosystems) using SYBR green as the detecting dye and Rox as the reference dye. The androgen responsive genes kalikrein 3, or PSA (KLK3) [Cleutjens, K. B., et al., Two androgen response regions cooperate in steroid hormone regulated activity of the prostate-specific antigen promoter. J Biol Chem, 1996. 271(11): p. 6379-88; Nelson, P. S., et al., The program of androgen-responsive genes in neoplastic prostate epithelium. Proc Natl Acad Sci USA, 2002. 99(18): p. 11890-5), metalloproteinase 16 (MMP16) (Nelson, P. S., et al., The program of androgen-responsive genes in neoplastic prostate epithelium. Proc Natl Acad Sci USA, 2002. 99(18): p. 11890-5), transmembrane protease serine 2 (TMPRSS2) (Nelson, P. S., et al., The program of androgen-responsive genes in neoplastic prostate epithelium. Proc Natl Acad Sci USA, 2002. 99(18): p. 11890-5; Aronin, N., et al., Are there multiple pathways in the pathogenesis of Huntington's disease? Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, 1999. 354(1386): p. 995-1003; Vaarala, M. H., et al., Expression of transmembrane serine protease TMPRSS2 in mouse and human tissues. J Pathol, 2001. 193(1): p. 134-40), FK506-binding immunophilin 51 (FKBP51) (Nelson, P. S., et al., The program of androgen-responsive genes in neoplastic prostate epithelium. Proc Natl Acad Sci USA, 2002. 99(18): p. 11890-5; Amler, L. C., et al., Dysregulated expression of androgen-responsive and nonresponsive genes in the androgen-independent prostate cancer xenograft model CWR22-R1. Cancer Res, 2000. 60(21): p. 6134-41; Velasco, A. M., et al., Identification and validation of novel androgen-regulated genes in prostate cancer. Endocrinology, 2004. 145(8): p. 3913-24; Magee, J. A., et al., Direct, androgen receptor-mediated regulation of the FKBP5 gene via a distal enhancer element. Endocrinology, 2006. 147(1): p. 590-8), G-protein coupled receptor RDC1 homolog, or chemokine orphan receptor 1 (RDC-1) (Nelson, P. S., et al., The program of androgen-responsive genes in neoplastic prostate epithelium. Proc Natl Acad Sci USA, 2002. 99(18): p. 11890-5), NK homeobox family member 3 (Nk×3.1) (Nelson, P. S., et al., The program of androgen-responsive genes in neoplastic prostate epithelium. Proc Natl Acad Sci USA, 2002. 99(18): p. 11890-5; Bieberich, C. J., et al., Prostate-specific and androgen-dependent expression of a novel homeobox gene. J Biol Chem, 1996. 271(50): p. 31779-82; Aboody-Guterman, K. S., et al., Green fluorescent protein as a reporter for retrovirus and helper virus free HSV-1 amplicon vector-mediated gene transfer into neural cells in culture and in vivo. Neuroreport, 1997. 8(17): p. 3801-8) were all normalized to the transcription of the housekeeping ribosomal gene (RPL19). All samples were done in triplicate and separately normalized. KLK3, Nk×3.1, TMPRSS2, and FKBP51 were all induced by treatment with DHT, and this induction was inhibited to varying degrees by BiC, PP, and HH. Likewise, MMP-16 and RDC-1 were repressed by treatment with DHT, and the repression was lifted to varying degrees by all of the AR inhibitors. Table 2 summarizes the results from 3 or 4 separate qRT-PCR experiments in each cell type. Two genes known to be induced by DHT in LNCaPs, Nk×3.1 and TMPRSS2, were not significantly induced in LAPC4 cells. Both PP and HH were observed to readily suppress expression of several androgen-responsive genes at least as effectively as BiC (Table 2).







TABLE 2







PP and HH each inhibit gene expression mediated by endogenous AR.


Both PP and HH reduced androgen-induced gene induction in a manner


comparable or superior to BiC. Evaluation of androgen-repressed


genes (shown in the bottom section) indicated that


the inhibitors each de-repressed expression.













No
3 nM
3 nM DHT/
3 nM DHT/
3 nM DHT/



DHT
DHT
1 uM BIC
100 nM PP
100 nM HH
















LNCaP







transcription


(normalized to


RPL19)


KLK3
1
1.89
1.53
1.57
1.46


Nkx3.1
1
2.00
1.31
0.78
1.28


FKBP51
1
2.15
3.56
1.46
1.90


TMPRSS2
1
3.70
2.58
0.92
1.22


MMP16
1
0.61
0.78
1.57
1.02


RDC1
1
0.66
0.66
0.94
1.22


LAPC4


transcription


(normalized to


RPL19)


KLK3
1
1.97
1.70
1.61
1.64


Nkx3.1
1
1.08
1.11
0.77
1.55


FKBP51
1
21.4
21.32
9.55
11.96


TMPRSS2
1
1.13
1.23
1.90
1.86


MMP16
1
0.71
2.32
2.92
4.74


RDC1
1
0.63
2.07
1.22
2.88









PP and HH are non-competitive antagonists. As a first step to determine whether PP and HH function as competitive or noncompetitive antagonists of AR, DHT was titrated in LNCaP cells transiently transfected with a PSA-luciferase reporter, and treated with a moderate dose of each inhibitor. DHT overcame the inhibitory effect of OH-F, producing maximal activation. By contrast, both PP and HH inhibited maximal DHT-induced activation, despite very high final DHT concentrations (FIG. 5). This was consistent with activity as non-competitive antagonists.
Example 5
Synergistic Behavior of PP, HH and BiC
The presence of synergy in the use of PP, HH and BiC as androgen receptor antagonists is confirmed using the procedure below.
The existence of synergy between the lead compounds and BiC was tested for using LAPC4 and LNCaP cells. When added in various combinations to LNCaP and LAPC4 cells transfected with the PSA-luciferase, both PP and HH synergized with each other, and with BiC (Table 3). Synergism can be defined as a combination index of less than one, using the non-exclusive assumption described by Chou et al. (Chou, T. C. and P. Talalay, Quantitative analysis of dose-effect relationships: the combined effects of multiple drugs or enzyme inhibitors. Adv Enzyme Regul, 1984. 22: p. 27-55). The synergy between PP, HH, and BiC is indicates that they function by distinct mechanisms.







TABLE 3







Synergy between PP, HH and BiC. Dose response curves were


carried out with the inhibitors in the indicated ratios, using 3 nM


DHT to activate transcription from PSA-Luciferase in the indicated


cell types (LNCaP or LAPC4). The expected IC50 value is reported


for the first drug indicated in the combination. The Combination


Index is a calculated measure of “synergy” based on Chou et al.


(1984); numbers lower than one indicate synergy.













Expected
Actual
Combination


Combination
Cell Type
IC50 (nM)
IC50 (nM)
Index at f50














PP:HH 1:1
LNCaP
9.27
8.27
0.54


PP:HH 1:1
LAPC4
7.83
4.57
0.28


PP:BIC 1:1
LNCaP
18.47
12.23
0.33


PP:BIC 1:1
LAPC4
18.25
0.64
0.02


HH:BIC 1:1
LNCaP
78.05
31.85
0.21


HH:BIC 1:1
LAPC4
142.1
28.33
0.1


PP:HH 1:10
LNCaP
26.79
2.56
0.08


PP:BIC 1:10
LNCaP
27.21
0.4
0.01


PP:BIC 1:30
LNCaP
27.69
10.25
0.44


PP:BIC 1:100
LNCaP
27.09
2.38
0.32


PP:OHF 1:1
LAPC4
7.56
5.79
0.39


Harmol:OHF
LAPC4
93.06
66.18
0.38









Example 6
Growth Inhibition by PP and HH
The procedures set-forth below were used to determine whether PP and HH would affect the androgen-dependent proliferation of LAPC4 and LNCaP cells in cell culture, as well as the androgen-independent growth of LN-AR cells that over-express AR, and which are a model of “androgen independent” prostate cancer.
LN-AR cells were created by retroviral infection of a high-expressing AR vector into LNCaP cells, and proliferate independent of androgen in cell culture, unlike their parental line (Chen, C. D., et al., Molecular determinants of resistance to antiandrogen therapy. Nat Med, 2004. 10(1): p. 33-9). HEK293 cells were used as a control. Cells were transferred to charcoal-stripped media two days before they were split and plated in quadruplicate at a density of approximately 20,000 cells/well in 48-well plates. The following day, medium with or without 3 nM DHT was added to the cells with or without PP (100 nM), HH (100 nM), or BiC (1 μM). Media were changed every day, using a single preparation to ensure consistent compound concentrations. Proliferation was determined by measuring the DNA content of the cells in each well. Each day, the cells in four wells were fixed in 100% cold methanol, followed by staining for 5 min at RT with 0.2 ng/mL 4′,6-diamidino-2-phenylindole (DAPI) in PBS. The cells were washed once in PBS, then read on a fluorescence plate reader using 365/439 excitation/emission wavelengths. LAPC4 and LNCaP cells proliferated to a much greater degree in the presence of DHT, while LN-AR cells proliferated in the presence and absence of DHT. PP was the only compound able to inhibit the growth of all four cell lines after seven days, while HH and BiC only inhibited significantly the growth of LNCaP cells. Control HEK293 cells were not significantly affected by any of the compounds, suggesting that none of them has non-specific growth-inhibitory effects. PP and HH worked at least as well as BiC to block androgen-induced proliferation (FIG. 6).
Example 7
Pharmacokinetics and Toxicity of Compounds in Mice
Anti-androgen activity of PP and HH in mice were determined using the procedure set forth below.
Wild-type FVB mice were given a single intraperitoneal injection of either PP or HH. Serum samples were obtained at fixed time intervals, and the drug levels determined by mass spectrometry. These results indicated that HH was rapidly cleared. PP exhibited a prolonged half-life indicating that it could be a suitable therapy (Table 4). Toxicity of PP was tested using various doses ranging from 0.1 to 10 mg/kg. 10 mg/kg was toxic, while the mice tolerated 5 mg/kg.







TABLE 4







Pharmacokinetics of PP vs. HH. Mice were given a single IP


injection or PO dose of PP or HH at 5 mg/kg. Serum samples


were drawn at the indicated time points, and blood levels


measured using mass spectrometry.












0.25 hr
1 hr
6 hr
24 hr











Plasma [PP] (ng/mL)













PO
40.2
13.5
nd
nd



IP
57.2
19.7
15.9
6.9







Plasma [HH] (ng/mL)













PO
6.7
nd
nd
nd



IP
32.3
 5.0
nd
nd










PP synergizes with BiC in vivo to cause prostate atrophy. As a positive control, one cohort was castrated four weeks prior to tissue recovery, one cohort was untreated, and the other cohorts were treated either with 1 mg/kg PP, 100 mg/kg BiC, or PP/BiC in combination. Animals were treated 5 times per week for four weeks. At the end of the study period, animals were sacrificed and prostate tissue weighed. After weighing, the tissue was divided in half for pathological and genetic studies. BiC treatment decreased prostate tissue size by 35% (p<0.003). Treatment with PP:BiC caused a further reduction by 63% that was highly significant vs. the effect of BiC alone (P<0.0005). PP alone did not produce a statistically significant effect. (FIG. 7).
PP synergizes with BiC in vivo to inhibit androgen-dependent prostate gene expression. To evaluate the effect of PP and PP:BiC on androgen-dependent gene expression in the prostate, total RNA was prepared from half of the prostate glands isolated in the in vivo trial. RT-PCR was carried out, and gene expression was determined relative to RPL19, an endogenous prostate gene that is completely androgen unresponsive (E. Bolton, K. R. Yamamoto: unpublished data). Five androgen-responsive genes that are expressed throughout all lobes of the mouse prostate were evaluated. In each case, it was observed that PP and BiC reduced gene expression significantly, but their combination was superior. In most cases, the combination approached that of castrated animals (FIG. 8). Taken together with the preceding experiment, PP exerts anti-androgen effects, and synergy with BiC as an anti-androgen in vivo.
Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, one of skill in the art will appreciate that certain changes and modifications may be practiced within the scope of the appended claims. In addition, each reference provided herein is incorporated by reference in its entirety to the same extent as if each reference was individually incorporated by reference.

## Claims

1. A method of inhibiting the androgen receptor, the method comprising:
administering to a patient in need thereof, a therapeutically effective amount of a compound of Formula Ic:




wherein
the dashed line is absent;
R1, R2 and R3 are each independently selected from the group consisting of hydrogen and C1-6 alkyl;
R4 is a member selected from the group consisting of hydrogen, C1-6 alkyl and C1-6 alkyl-OH;
R5 is a member selected from the group consisting of hydrogen, C1-6 alkyl and —NR6R7;
R6 and R7 are each independently selected from the group consisting of hydrogen and C1-6 alkyl, or are combined with the nitrogen to which they are attached to form a heterocycloalkyl having from 5 to 7 ring members;
and salts thereof, thereby inhibiting the androgen receptor.

2. The method of claim 1, wherein
R1 is H; and
R2, R3, R4 and R5 are each C1-6 alkyl.

3. The method of claim 1, wherein the compound is:

4. The method of claim 1, wherein the salt forms comprise a counterion selected from the group consisting of pamoate, chloride, bromide, succinate, maleate and acetate.

5. The method of claim 1, wherein the patient suffers from a disease selected from the group consisting of prostate cancer, ovarian cancer, acne vulgaris, breast cancer, polycystic ovary syndrome, benign prostatic hyperplasia, alopecia, and hirsutism.

6. The method of claim 5, wherein the disease is prostate cancer.

7. The method of claim 6, wherein the disease is primary prostate cancer.

8. The method of claim 6, wherein the disease is hormone refractory prostate cancer.

9. The method of claim 1, wherein the administration is via topical, oral, intravenous, intradermal, intramuscular or parenteral administration.

10. The method of claim 5, wherein the disease is alopecia and the administration is topical.

11. The method of claim 1, wherein the compound of Formula Ic is administered with a course of hormonal therapy, wherein the compound for hormonal therapy is selected from the group consisting of an anti-androgen and a LnRH agonist.

12. The method of claim 11, wherein the compounds are administered separately.

13. The method of claim 11, wherein the compounds are admixed.

14. The method of claim 11, wherein the compounds are administered at the same time.

15. The method of claim 11, wherein the compounds are administered at different times.

16. The method of claim 11, wherein the compound of Formula Ic is administered in combination with a therapeutically effective amount of a compound selected from the group consisting of bicalutamide, flutamide, hydroxyflutamide, nilutamide, spionolactone, cyproterone acetate, ketoconazole, finasteride and dutasteride.

17. The method of claim 11, wherein the compound of Formula Ic is administered in combination with a therapeutically effective amount of a coumarin.

18. A pharmaceutical composition comprising a therapeutically effective amount of a compound of formula Ic:



wherein
the dashed line is absent;
R1, R2 and R3 are each independently selected from the group consisting of hydrogen and C1-6 alkyl;
R4 is a member selected from the group consisting of hydrogen, C1-6 alkyl and C1-6 alkyl-OH;
R5 is a member selected from the group consisting of hydrogen, C1-6 alkyl and —NR6R7;
R6 and R7 are each independently selected from the group consisting of hydrogen and C1-6 alkyl, or are combined with the nitrogen to which they are attached to form a heterocycloalkyl having from 5 to 7 ring members;

in combination with a therapeutically effective amount of a compound selected from the group consisting of an anti-androgen and a LnRH agonist.