ATP Case No. N09-004 Study Report 

1

ATP Study Report for the Proposed US EPA Approval of 

Colilert® and Colilert®-18 for the Detection and Enumeration of 

Fecal Coliforms in US Wastewater Samples 

February 1, 2010 

IDEXX Laboratories, Inc. 

Westbrook, Maine 04092
ATP Case No. N09-004 Study Report 

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Background 

Introduction: 

This report is intended to review the data collected to compare the performance 

of the Colilert and Colilert-18 test methods against the Standard Method (i.e. 

SM9222D) from Standard Methods for the Examination of Water and Wastewater

for detecting fecal coliform bacteria in wastewater samples (Appendix D, 3). 

Colilert and Colilert-18 are US EPA approved for the detection and quantification 

of total coliforms & Escherichia coli from source and drinking water samples. 

Both methods are also approved for the specific detection and quantification of E. 

coli from wastewater samples. This report describes a study that was initiated 

following the US EPA 2004 Alternative Test Procedure (ATP) guidance protocol 

(Appendix D, 1) to evaluate the performance of both methods at detecting and 

quantifying fecal coliform bacteria in support of a US EPA approval initiative. 

Both Colilert and Colilert-18 are manufactured by IDEXX Laboratories, inc. 

located in Westbrook, Maine (Appendix A(1) and Appendix A(2)) 

Data generated for this study were collected in accordance with the ATP Study 

Plan approved by the US EPA on July 21st, 2009 (Appendix B).

Justification: 

Colilert and Colilert-18 can detect and quantify the concentration of total coliform 

& E. coli bacteria from potable water samples and the concentration of E. coli

from wastewater samples after 24 and 18 hours, respectively. Both methods 

offer considerable advantages to laboratory analysts in terms of their ease of 

use, accuracy, and time to results over SM9222D and have been widely adopted 

in most public and private laboratories for US EPA mandated testing. Although 

40 CFR 136.3, Table IA—List of Approved Biological Methods for Wastewater 

and Sewage Sludge (Appendix D, 2), allows for the use of E coli as one of a list 

of allowable target organisms under the rule, some states have not yet approved 

the use of E. coli monitoring of wastewater discharge and, as a result, can not 

use Colilert and Colilert-18 for wastewater monitoring. Therefore, IDEXX initiated 

this study to obtain US EPA approval for fecal coliform testing for both Colilert 

and Colilert-18 to allow these states access to these methods. 

Method Summary: 

The Colilert and Colilert-18 tests are manufactured in snap packs; one snap pack 

contains enough powder to perform one test for the presence of fecal coliform 

bacteria in 100mL of wastewater. The user simply adds the contents of one of 

the snap pack to a vessel containing 100mL of a wastewater sample followed by 

shaking to quickly dissolve the powder into the liquid reagent. After shaking, the 

reagent is transferred to a sterile Quanti-Tray device (Quanti-Tray or QuantiTray/2000 from IDEXX Laboratories, Inc.) which dispenses and seals the reagent 

into a large number of incubating wells. The Quanti-Tray is then incubated at 

44.5 +/-0.2°C for 24 hours (when using Colilert) or 18 hours (when using Colilert18). After incubation, the number of wells which have changed color to yellow 

are counted and compared against a most probable number (MPN) chart 
ATP Case No. N09-004 Study Report 

3

(provided with the Quanti-Trays) to arrive at the final number of fecal coliform 

bacteria detected in the original wastewater sample. 

Equivocal samples are compared for color change against a color comparator 

(IDEXX Laboratories Inc.) In the Study Method, fecal coliform detection is based 

on the presence of the enzyme ß-D-galactosidase which is known to be present 

in fecal coliform bacteria (Appendices A(1) and A(2)) For the detection of ß-Dgalactosidase, Colilert and Colilert-18 utilize the chromogenic nutrient indicator 

ortho-nitrophenyl-ß-D-galactopyranoside (ONPG) which produces a distinct 

yellow color when hydrolyzed by ß-D-galactosidase. The presence of this yellow 

color under these incubation conditions is highly specific for the detection of fecal 

coliform bacteria. 

Study Objectives and Design 

Main Objectives: 

The main objective of this ATP study was to demonstrate the comparability of the 

Colilert and Colilert-18 methods to a US EPA approved method (SM9222D) for 

compliance monitoring of fecal coliform discharge from wastewater facilities. 

IDEXX Laboratories, Inc. carried out this ATP study in support of their request for 

nationwide approval of Colilert and Colilert-18 by the US EPA. 

Data Quality Objectives: 

IDEXX coordinated and served as the Study Director for this ATP after approval 

of the proposed study plan by the US EPA. The data was collected and recorded 

at an independent certified laboratory approved by the US EPA which has 

provided a bound copy of all the data collected during this study (Appendix C). 

Study Design: 

The design of this study was based on the US EPA’s Microbiological Alternative 

Test Procedure Protocol for Drinking Water, Ambient Water and Wastewater, 

version 2004 and is described in more detail in the US EPA approved protocol 

(Appendix B). In summary, primary wastewater effluent samples were collected 

from 10 different locations within the New England area and used as sources of 

fecal coliform bacteria for this study. The effluent samples were collected at the 

individual wastewater treatment plants, processed, and tested at the certified 

laboratory within six hours. If necessary, the effluent samples were diluted to 

achieve a fecal coliform innoculum of approximately 20 to 50 organisms per 

100mL of sample. Each sample was analyzed by Colilert, Colilert-18, and 

SM9222D in replicates of twenty after incubation at 44.5+/-0.2°C for the 

appropriate period of time. Sensitivity for fecal coliform detection was 

determined by comparing the number of presumptive positive responses for the 

Colilert and Colilert-18 methods against the number of presumptive positive 

responses from SM9222D. Specificity was determined by confirming the identity 

at least 200 presumptive positive and 200 presumptive negative determinations 

from each of the three test methods. These data were used to determine the 

statistical comparability of the fecal coliform counts determined by each method 

following the ATP guidelines as well as the confirmed false positive and false 

negative rates. 
ATP Case No. N09-004 Study Report 

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Study Implementation 

Study management: 

All laboratory work for this study, except for VITEK identifications, was carried out 

at Analytical Services, Inc. (ASI) located in Williston, Vermont. IDEXX served as 

the Study Director and obtained approval of the Study Plan and the writing of the 

Study Report. ASI was solely responsible for collecting the effluent samples, 

running the experiments, recording the data, and maintaining all QA/QC 

procedures specified in the US EPA approved study protocol. As specified in the 

US EPA approved study protocol, some bacterial isolates were sent to IDEXX for 

final confirmation using the VITEK bacterial identification system. These 

samples were coded at ASI and shipped to IDEXX with a well documented chain 

of custody. Frozen archived samples for each bacterial isolate shipped to IDEXX 

are being maintained at ASI. 

Type of study: 

The study was a side-by-side method comparison comparing each Colilert and 

Colilert-18 against SM9222D, an EPA-approved reference method, to determine 

whether the performance of both Colilert and Colilert-18 are acceptable 

compared to the reference method. 

Study Schedule: 

The data for this study was collected during the months of October, November, 

and December 2009. ASI was responsible for all aspects of the study and 

reported the results to the Study Director on a weekly basis. All effluent samples 

were tested at ASI within 6 hours of collection. 

Matrix and spiking source: 

The spiking source was primary sewage effluent from wastewater treatment 

plants located in the New England area. These samples were collected and 

tested according the US EPA’s Microbiological Alternative Test Procedure 

Protocol for Drinking Water, Ambient Water, and Wastewater, Version 2004 

(Appendix D, 1). 

Preliminary testing: 

Training was conducted at ASI during October 2009. Training consisted of 

assessing ASI’s ability to accurately follow the US EPA approved study protocol 

and to ensure that all processes and procedures were well documented and 

followed good QA/QC practices. Data collected by ASI during this training 

demonstrated strong technical competence, excellent documentation, and strong 

quality standards. 

Numbers and types of analyses: 

Ten effluent samples were collected from ten different sewage treatment facilities 

located in the New England area and used as fecal coliform sources for Colilert 

and Colilert-18 vs. SM9222D comparability studies. 

Sample collection, distribution, and storage: 

Samples were collected in bottles containing preservatives as outlined in 

Standard Methods for the Examination of Water and Wastewater, 20th Edition, 
ATP Case No. N09-004 Study Report 

5

section 9060 A.2 (Appendix D, 2). Samples were couriered to the laboratory in 

coolers packed with ice or “blue ice”. As required in 40 CFR 136.3(e) Table II, 

samples were analyzed within 6 hours of collection (Appendix D, 2). Chemical 

characterization of the wastewater samples was not required. 

Data Reporting and Validation 

Quality Assurance/Quality Control:

QA/QC procedures were followed as specified in the US EPA approved Study 

Plan (Appendix B) and consistent with the guidelines of a certified laboratory. 

Control cultures: 

Each lot of medium was checked to verify the performance with known positive 

and negative bacterial samples. Details of this testing can be found in the bound 

data package provided by ASI (Appendix C). 

Results 

Fecal coliform recovery by each test method can be found in Tables 1 to 10. 

1 64.4 67.0 19.0

2 63.1 53.0 32.0

3 54.6 68.4 22.0

4 59.4 35.9 28.0

5 41.3 37.9 24.0

6 51.2 43.5 25.0

7 52.0 55.6 23.0

8 57.3 38.9 39.0

9 67.7 52.1 32.0

10 54.6 36.4 19.0

11 59.8 32.7 42.0

12 40.4 56.8 40.0

13 55.4 52.9 25.0

14 52.0 69.7 18.0

15 48.7 55.6 22.0

16 31.3 30.9 26.0

17 41.3 45.5 29.0

18 65.7 42.8 41.0

19 63.1 63.1 27.0

20 48.0 53.0 25.0

Table 1

Fecal Coliform Recovery from Bethel, ME Effluent

Replicate Colilert-18

(MPN/mL)

Colilert

(MPN/mL)

SM9222D

(cfu/mL)

Average 53.57 49.59 27.90
ATP Case No. N09-004 Study Report 

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1 83.6 38.4 17

2 62.7 47.1 61

3 69.1 24.9 49

4 71.7 39.9 44

5 61.3 51.2 55

6 69.7 28.5 41

7 59.4 59.1 41

8 62.4 29.2 29

9 60.5 34.1 38

10 52.1 61.7 29

11 55.6 52 30

12 93.3 36.8 46

13 57.3 55.6 57

14 63.1 47.1 36

15 55.6 39.3 31

16 58.6 34.1 31

17 61.3 48 50

18 83.6 59.4 33

19 68.3 42.6 43

20 65.7 36.4 30

Average 65.75 43.27 39.55

Table 2

Fecal Coliform Recovery from Burlington, VT Effluent

Replicate Colilert-18

(MPN/mL)

Colilert

(MPN/mL)

SM9222D

(cfu/mL)
ATP Case No. N09-004 Study Report 

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1 53.8 62.4 35

2 60.5 78.9 45

3 68.3 60.5 48

4 47.1 79.4 61

5 70.6 83.6 45

6 50.4 75.4 40

7 60.1 53.8 50

8 54.6 71.7 40

9 53.8 54.7 49

10 40.4 63.1 42

11 60.5 73.3 41

12 56.1 65 49

13 64.4 71.7 39

14 63.1 57.3 45

15 68.3 72.2 42

16 72.7 63.7 44

17 64.4 62.4 57

18 52 52.1 46

19 45.5 60.5 53

20 53 54.7 48

Average 57.98 65.82 45.95

Table 3

Fecal Coliform Recovery from W. Lebabnon, NH Effluent

Replicate Colilert-18

(MPN/mL)

Colilert

(MPN/mL)

SM9222D

(cfu/mL)

1 44.8 78.4 31

2 63.7 80.9 45

3 70.6 63.8 53

4 48.7 56.1 42

5 49.6 71.2 43

6 41.3 62.4 42

7 59.1 66.3 49

8 46.4 74.3 45

9 32.7 69.7 33

10 74.9 52.8 44

11 54.6 58.3 34

12 52 51.2 38

13 53.8 69.1 34

14 45.7 64.4 40

15 52.1 66.3 57

16 38.9 40.4 45

17 50.4 43.5 51

18 41.9 44.1 49

19 55.7 52.1 47

20 40.4 66.3 48

Average 50.87 61.58 43.50

Table 4

Fecal Coliform Recovery from Keene, NH Effluent

Replicate Colilert-18

(MPN/mL)

Colilert

(MPN/mL)

SM9222D

(cfu/mL)
ATP Case No. N09-004 Study Report 

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1 45 54.6 53

2 47.9 45 43

3 36.8 52 54

4 47.9 34.1 51

5 49.5 38.4 41

6 32.3 45.5 48

7 45 41.9 51

8 33.1 36.9 47

9 36.4 42.6 47

10 44.1 33.6 59

11 44.8 38.4 37

12 35 45.7 41

13 48.7 40.4 44

14 49.5 45.7 16

15 45.7 31.7 33

16 48.7 41.9 43

17 26.9 59.8 44

18 31.3 52 42

19 45 60.1 47

20 46.4 49.5 26

Average 42.00 44.49 43.35

Table 5

Fecal Coliform Recovery from S. Burlington, VT Effluent

Replicate Colilert-18

(MPN/mL)

Colilert

(MPN/mL)

SM9222D

(cfu/mL)

1 44.8 65.1 29

2 71.7 65 37

3 68.3 65.7 42

4 65 66.3 32

5 48.7 63.7 30

6 32.8 63.1 42

7 52.9 73.3 39

8 54.7 77.6 33

9 55.6 65 41

10 47.9 69.7 36

11 56.5 55.7 29

12 46.4 69.7 9

13 53.8 57.3 29

14 47.1 83.6 37

15 52.1 63.1 29

16 62.4 78.9 36

17 40.4 69.1 28

18 43.5 55.6 26

19 55.6 70.3 19

20 41.9 76.6 34

Average 52.11 67.72 31.85

Table 6

Fecal Coliform Recovery from Essex Junction, VT Effluent

Replicate Colilert-18

(MPN/mL)

Colilert

(MPN/mL)

SM9222D

(cfu/mL)
ATP Case No. N09-004 Study Report 

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1 53.7 82 35

2 75.4 43.5 48

3 63.1 56.5 31

4 69.7 63.8 36

5 39.3 36.4 44

6 63.1 58.3 35

7 60.1 37.3 45

8 64.4 60.5 41

9 66.3 44.1 39

10 59.4 46.4 39

11 41.9 60.1 39

12 65 52.9 31

13 50.4 61.7 55

14 50.4 71.7 44

15 49.6 61.3 39

16 66.3 56.3 54

17 73.3 55.6 46

18 53.7 59.4 32

19 43.2 50.4 45

20 60.1 44.8 38

Average 58.4 55.2 40.8

Table 7

Fecal Coliform Recovery from Woodsville, NH Effluent

Replicate Colilert-18

(MPN/mL)

Colilert

(MPN/mL)

SM9222D

(cfu/mL)

1 57.3 74.4 24

2 54.6 76.6 26

3 44.8 80.5 31

4 41.3 99 26

5 57.3 60.5 22

6 30.9 63.7 25

7 36.4 69.1 33

8 34.1 69.1 34

9 53.8 51.2 18

10 49.6 52.1 16

11 33.5 52.9 16

12 37.3 61.3 25

13 52 45.7 16

14 23.3 53 22

15 34.5 55.6 20

16 33.5 30.5 25

17 29.9 49.5 24

18 42.2 39.3 22

19 35 45.5 21

20 27.8 39.3 26

Average 40.5 58.4 23.6

Table 8

Fecal Coliform Recovery from Northhampton, MA Effluent

Replicate Colilert-18

(MPN/mL)

Colilert

(MPN/mL)

SM9222D

(cfu/mL)
ATP Case No. N09-004 Study Report 

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1 53.9 59.1 35

2 69.1 65.7 34

3 40.8 54.7 32

4 44.8 96 38

5 62.9 73.8 37

6 71.2 61.3 28

7 60.5 73.8 36

8 45.5 63.1 41

9 79.4 66.9 42

10 59.4 77.1 39

11 60.1 78 28

12 62.4 90.7 42

13 44.8 78 44

14 63.7 71.7 34

15 64.4 63.8 40

16 50.4 70.6 41

17 55.6 64.4 37

18 44.1 62.9 38

19 56.5 105 44

20 71.2 71.7 32

Average 58.0 72.4 37.1

Table 9

Fecal Coliform Recovery from Winthrop, MA Effluent

Replicate Colilert-18

(MPN/mL)

Colilert

(MPN/mL)

SM9222D

(cfu/mL)

1 33.1 96 40

2 47.1 98.7 42

3 41.6 98.8 43

4 33.5 93.3 52

5 37.9 77.6 39

6 28.8 49.6 41

7 36.8 81.6 44

8 41 85.5 51

9 37.9 81.6 55

10 44.8 90.7 54

11 34.1 60.1 26

12 31.3 77.1 42

13 44.8 69.7 54

14 41.3 86.2 58

15 28.8 105.4 34

16 33.5 64.4 50

17 42.8 74.9 35

18 25.6 63.8 41

19 42.6 88 51

20 36.9 76.6 40

Average 37.2 81.0 44.6

Table 10

Fecal Coliform Recovery from Littleton, NH Effluent

Replicate Colilert-18

(MPN/mL)

Colilert

(MPN/mL)

SM9222D

(cfu/mL)
ATP Case No. N09-004 Study Report 

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Data Analysis and Discussion 

Fecal coliform testing:

 

The ability to recover natural fecal coliform populations was assessed for Colilert, 

Colilert-18 and SM9222D using primary effluent samples collected from ten 

different wastewater treatment facilities located in the New England area (Table 

11). Each test method was spiked with a primary effluent solution containing 

approximately 20 to 50 fecal coliforms per 100mL in replicates of twenty per 

effluent site. The test samples were incubated at 44.5 ± 0.2oC for the designated 

period of time and analyzed to determine the presumptive fecal coliform 

population recovered by each method. The raw data for fecal coliform recovery 

from this study can be found in Appendix C. The recovery data was analyzed to 

assess the normality, precision, and recovery characteristics of each test 

method. 
ATP Case No. N09-004 Study Report 

Assessment of normality:

 Fecal coliform recovery data for Colilert, Colilert-18, and SM9222D were 

tested for normality with each effluent sample using the Kolmogorov-Smirnov test 

(Table 12). Analysis of these data indicated fecal coliform recovery by all three 

methods was consistent with a normal distribution with p-values in excess of 

0.366 for all samples with the exception of the Colilert-18 method, which had a pvalue of 0.119 with the South Burlington, Vermont effluent. Even this p-value is 

greater than the usual threshold of 0.10. These results confirm that the 

assumption of normality has been met for all three test methods. According to 

the ATP guidance document (Appendix D, 1) quantitative methods that follow a 

normal distribution need to be compared for variances between the effluent 

samples to determine if the recovery variance is homogeneous across samples. 

Assessment of precision: 

Variance across ten effluent sites 

 The variance homogeneity in fecal coliform recovery across all matrices 

for each test method was tested using the Bartlett’s test (Table 13). For the 

Colilert-18 method, the variances across the ten sites were similar with a p-value 

of 0.308. For Colilert and SM9222D the variances across the ten sites were not 

homogeneous with p-values of 0.029 for Colilert and 0.006 for SM9222D. 

Additional analysis using Levene’s test shows good internal consistency (p > 

0.05) with the Colilert and Colilert-18 tests but not with SM9222D. Overall, the 

internal consistency was lowest with SM9222D, which seems to underscore the 

difficulty in interpreting the results of this method with true environmental 

samples. Microbiological methods that utilize morphological characteristics of 

colonies as a means to detect a particular bacterial type are prone to this kind of 

inconsistency given the subjectivity of this approach. The lack of internal 

consistency required that the variances between methods be compared 

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ATP Case No. N09-004 Study Report 

separately for each effluent sample instead of analyzing the pooled variances 

from the ten effluent samples tested as per ATP guidelines. 

Colilert-18 and Colilert vs. SM9222D 

 The variance equality in fecal coliform recovery between Colilert-18 and 

SM9222D was tested using the F-test assuming a normal distribution in recovery 

with each effluent sample. The data presented in Table 14 show that fecal 

coliform recovery by the Colilert-18 method was statistically equivalent (p > 0.05) 

to SM9222D with 80% of the effluent samples tested. SM9222D showed higher 

precision with two of the effluent samples (Northampton and Winthrop, 

Massachusetts). 

 The differences between variances between Colilert and SM9222D were 

slightly more significant. The data presented in Table 15 show that six out of the 

ten sites displayed similar variances (p>0.05) between the two methods while the 

remaining four sites (Northampton, Winthrop, Littleton, and Woodsville) did not. 

As noted above, SM9222D had the lowest overall precision across the ten 

effluent samples tested by the three methods. This internal inconsistency 

complicates the comparison of precision between the methods because such an 

analysis assumes that SM9222D would pass this analysis. Given the relatively 

poor precision of SM9222D in this study it’s difficult to interpret what a 

comparison of its precision against the Colilert and Colilert-18 methods really 

means. 

 As previously mentioned, the results from SM9222D are difficult for an 

analyst to read and interpret in a consistent manner. Fecal coliform bacteria form 

colonies on the m-FC medium presenting colonies containing “various shades of 

blue”, which can differ in intensity based on the metabolic state of the bacterium. 

This detection strategy introduces considerable subjectivity into reading the 

results of this test based on the analysts’ interpretation of what constitutes a 

shade of blue. Furthermore, non-fecal coliform bacteria grow on the m-FC 

medium and produce colored colonies ranging from gray to cream or even yellow 

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ATP Case No. N09-004 Study Report 

that can interfere with the read. The presence of these competing bacterial 

populations deprives fecal coliforms of important nutrients and depresses their 

metabolic functions, thereby, decreasing the intensity of the blue color signal. It 

is for this reason that SM9222D requires all shades of blue be counted as 

presumptive positive for fecal coliform detection. In contrast, the Colilert and 

Colilert-18 methods produce results that are unambiguous. With Colilert and 

Colilert-18 the presence of fecal coliform bacteria is indicated by a distinct color 

change in the wells of the Quanti-Tray device to yellow. This color change is 

easy to read and interpret which is supported by the relatively high precision data 

collected in this study, especially with the Colilert-18 method. Finally, non-fecal 

coliform bacteria are rarely able to grow and be detected by these test methods 

so the intensity of the signal is more consistent than seen with SM9222D due to 

the lack of competing flora. 

 Based on the above considerations, Colilert and Colilert-18 methods 

maintained superior internal consistency over SM9222D across all the effluents 

samples tested. Inherent inconsistencies seen with SM9222D preclude an 

accurate assessment of the comparable variances between methods. 

Assessment of recovery: 

Colilert-18 vs. SM9222D 

 The ATP guidance document specifies that, when the assumption of 

normality is not violated, the analyte recovery be first analyzed to determine if 

significant interaction between method & matrix occurred in the study. A Two 

Way ANOVA analysis was used for this determination across all ten matrices, 

which revealed that a significant interaction (p = 0.00) was present between the 

test methods and effluent samples (Appendix C). As a result, the fecal coliform 

recovery comparison was made 

14
ATP Case No. N09-004 Study Report 

between methods with each independent effluent sample using a two sample ttest (a special case of ANOVA). The results of this analysis can be found in 

Table 16 and clearly show a bias in favor of Colilert-18 over SM9222D. Of the 

ten effluents tested by this method, Colilert-18 was found to recover a statistically 

larger population of fecal coliform bacteria (p < 0.05) than the SM9222D in 80% 

of the effluents tested. Colilert-18 was found to recover an equivalent amount of 

fecal coliform bacteria (p = 0.623) from the South Burlington, Vermont effluent 

and a statistically smaller population (p = 0.003) from the Littleton, New 

Hampshire effluent. 

Colilert vs. SM9222D

 The results of this analysis (Table 17) showed a clear bias in favor of the 

Colilert method. Colilert was able to recover a statistically larger population of 

fecal coliform bacteria than SM9222D (p < 0.05) in 80% of the effluents tested. 

The remaining effluents (South Burlington & Burlington) yielded equivalent fecal 

coliform recovery (p > 0.05). 

 Both Colilert and Colilert-18 out performed SM9222D at recovering fecal 

coliform bacteria from at least 80% of the effluent samples tested. One factor 

that may account for some of the differences seen in fecal coliform recovery 

during this study is the discovery that SM9222D tended to underestimate the 

concentration of fecal coliforms recovered on the m-FC medium. As will be 

discussed in more detail below, SM9222D is prone to a much higher false 

negative rate than the other two methods most likely due to inherent difficulties in 

reading the results off the m-FC medium as previously described. 

Assessment of the false positive and false negative rates:

False positive 

 Presumptive positive responses from each of the test methods were 

harvested for further characterization following the methodology described in 

15
ATP Case No. N09-004 Study Report 

Figure 1. A minimum of 200 presumptive positive responses from each test 

method were subjected to this confirmation process to verify the accuracy of 

each test. For the purposes of this study a fecal coliform was defined as: any 

member of the Escherichia, Klebsiella, Enterobacter, or Citrobacter genus 

capable of growing at 44.5 ± 0.2oC. This definition was applied equally to 

Colilert, Colilert-18, and SM9222D. The results of this study can be found in 

Table 18. All three test methods had low presumptive false positive rates 

ranging from 0 to 1.5%. The majority of the presumptive positive samples were 

confirmed as being true fecal coliform bacteria by the EC broth method. 

However, less than 25% of the samples required further characterization by the 

VITEK bacterial identification system because they failed to generate gas in the 

EC medium when grown at 44.5 ± 0.2oC. The identity of these anaerogenic fecal 

coliform bacteria can be found in Tables 19, 20 and 21 and the majority of the 

time they were determined to be either Escherichia coli or Klebsiella 

pneumoniae. 

 Table 22 lists the identity of the confirmed false positive isolates detected 

by each test method. Two of the Colilert false positive samples were identified 

as Raoultella planticola that formerly belonged to the Klebsiella genus. The 

VITEK confidence level was lower than the 90% threshold specified by the study 

protocol (was found to be 85%) so they were designated as false positive 

responses. The same occurred with the one false positive sample detected by 

SM9222D which technically fell below the threshold of a fecal coliform as defined 

by the study protocol. 

False negative

 Presumptive negative responses from each of the test methods were 

harvested for further characterization following the methodology described in 

Figure 2. A minimum of 200 presumptive negative responses from each test 

method were subjected to this confirmation process to verify the accuracy of 

each test to correctly identify true negative samples. The results of this study 

16
ATP Case No. N09-004 Study Report 

can be found in Table 23 and show clear differences between the three test 

methods. The confirmed false negative rates for the Colilert and Colilert-18 

methods were found to be 9.0% and 7.0%, respectively. These values are 

consistent with published reports and are comparable to each other in this study. 

The VITEK identifications of these true false negative samples can be found in 

Tables 24, 25 and 26 and are summarized in Table 27 by genus type. All the 

false negative samples belonged to either the Escherichia or Klebsiella genus 

with no specific pattern evident with regard to effluent type. Three quarters of the 

Colilert false negative responses belonged to the Escherichia genus and the 

remaining quarter belonged to the Klebsiella genus. The opposite distribution 

was found with the Colilert-18 false negative samples. The confirmed false 

negative rate of SM9222D was 21.5%, which far exceeded even the combined 

rates of both the Colilert and Coliert-18 methods. As summarized in Table 27, 

the majority of the confirmed false negative responses for SM9222D belonged to 

the genus Escherichia. 

 The relatively high false negative rate of SM9222D may explain the lower 

recovery rate of fecal coliform bacteria observed with this method as compared 

to Colilert and Colilert-18. More fecal coliforms may have been present on the 

m-FC medium that did not display the typical blue colony morphology specified in 

the method so they were not included in the presumptive fecal coliform count. 

The lack of a distinctive blue colony formation is possibly due to the presence of 

non-target bacteria that compete for nutrients, and therefore, interfere with the 

growth and development of the fecal coliform colonies. It is also possible that the 

strains that caused the false negative reaction were weak to moderate lactose 

fermenting strains were are not able to produce sufficient acid to react with the 

m-FC indicator to produce the typical blue color. Weak lactose fermenting 

strains were commonly encountered when confirming the presumptive positive 

samples (see above). These strains failed to be confirmed with EC broth, which 

depends upon lactose fermentation, and required subsequent confirmation by the 

Vitek method to verify the fecal coliform identity. The data presented in Table 18

showed that 33 out of the 200 (16.5%) presumptive positive samples obtained 

17
ATP Case No. N09-004 Study Report 

from SM9222D were not able to ferment the lactose in EC broth. This value is 

similar to the confirmed false negative rate for SM9222D and is consistent with 

this explanation. 

 Study results indicate that Colilert, Colilert-18, and SM9222D maintained a 

very low and highly comparable false positive rate for the recovery of fecal 

coliform bacteria from the effluent samples tested. However, the inherent 

variability of SM9222D coupled with its inability to consistently detect low lactose 

fermenting fecal coliforms resulted in a confirmed false negative rate which is 

significantly higher than the more accurate Colilert and Colilert-18 methods. 

Conclusions:

 Study results indicate that both the Colilert and Colilert-18 methods have 

met the US EPA acceptance criteria and have been shown to be equivalent or 

better than SM9222D for detecting and quantifying fecal coliforms in wastewater 

samples. Both methods displayed a normal distribution for fecal coliform 

recovery and maintained a statistically better internal consistency (i.e. precision) 

across the ten effluent samples tested than SM9222D. The lack of precision with 

method SM9222D complicated the side by side comparisons of the relative 

precision between methods therefore; no meaningful conclusion could be made. 

 Fecal coliform recovery by Colilert and Colilert-18 was statistically better 

than SM9222D with at least 80% of the individual effluent samples when the site 

specific recovery was compared. The higher overall recovery of fecal coliform 

bacteria by Colilert and Colilert-18 was not due to the presence of false positive 

responses as both methods exhibited false positive rates of less than 2%. 

However, the relatively high false negative rate of SM9222D (21.5%) seemed to 

play a major role in the recovery differences. Detection of presumptive positive 

fecal coliform bacteria by SM9222D relies on active lactose fermentation that 

was found to be absent in 16.5% of the strains encountered in this study. These 

anaerogenic fecal coliforms primarily belonged to the Escherichia and Klebsiella 

genus so they represented true fecal coliform bacteria and should have been 

18
ATP Case No. N09-004 Study Report 

19

detected. Reading results from SM9222D is also highly subjective and can be 

complicated by the presence of competing bacterial populations that diminish the 

intensity of the blue signal on m-FC medium. Evidence for this subjectivity was 

found in the low precision data collected from SM9222D and from personal 

communications from the analysts running the study. 
ATP Case No. N09-004 Study Report 

STATE CITY LOCATION SHIPPING 

VERMONT ESSEX JUNCTION ESSEX JUNCTION WWTF 2 Lincoln Street, Ess

VERMONT SOUTH BURLINGTON CITY OF SOUTH BURLINGTON WWTP 1015 Airport ParkwayVERMONT BURLINGTON BURLINGTON MAIN WWTP 53 Lavalley Lane, B

NEW HAMPSHIRE WOODSVILLE AOS WOODSVILLE WWTF 9 Armory Lane, W

NEW HAMPSHIRE WEST LEBANON WEST LEBANON WWTP 130 South Main Street, 

NEW HAMPSHIRE LITTLETON AOS LITTLETON WWTP 323 Meadow StreeNEW HAMPSHIRE KEENE KEENE WWTP 420 Airport Road, Rou

MAINE BETHEL BETHEL WWTP 199 Main Street, 

MASSACHUSETTS NORTHAMPTON NORTHAMPTON WWTP 33 Hockanum Road., 

MASSACHUSETTS WINTHROP MWRA (Deer Island Treatment Plant) Central Lab, 190 Tafts Av

Table 11

Primary Sewage Effluent Sample Sources

20
ATP Case No. N09-004 Study Report 

Bethel

Maine 37053 0.983 0.873 0.756

Northampton

Massachusetts 37266 0.619 0.885 0.602

Winthrop

Massachusetts 37265 0.88 0.538 0.995

Keene

New Hampshire 37117 0.925 0.896 0.955

Littleton

New Hampshire 37435 0.858 0.998 0.806

W. Lebanon

New Hampshire 37188 0.989 0.832 0.961

Woodsville

New Hampshire 37365 0.841 0.894 0.732

Burlington

Vermont 37054 0.407 0.93 0.905

Essex Junction

Vermont 37189 0.909 0.916 0.651

S. Burlington

Vermont 37202 0.119 0.819 0.366

Table 12

Kolmogorov-Smirnov normality test p-values for data corresponding

 to different sites and methods

Effluent site

tested

P-value

(Colilert-18)

P-value

(Colilert)

P-value

(SM9222D) ASI effluent ID

 

0.125

0.042

Table 13

Test of variance equality for each method across the 10 sites

 based on Bartlett’s test & Levene’s test p-values

Colilert

Method 9222D

0.308

0.029

0.006

Method P-value

(Barlett's test)

P-value

(Levene's test)

Colilert-18 0.619

21
ATP Case No. N09-004 Study Report 

Table 14

F-test p-values for comparison of variances between Colilert-18 and SM9222D

Effluent site

 tested ASI effluent ID Std. Dev (Colilert-18)

Std. Dev 

(SM9222D) P-value

0.279

Northhampton

Massachusetts 37266 10.441 5.165 0.004

Bethel

Maine 37053 9.662 7.504

0.001

Keene

New Hampshire 37117 10.495 6.970 0.082

Winthrop

Massachusetts 37265 10.602 4.756

0.178

W. Lebanon

New Hampshire 37118 8.784 6.253 0.148

Littleton

New Hampshire 37435 6.027 8.262

0.084

Burlington

Vermont 37054 10.550 11.232 0.788

Woodsville

New Hampshire 37365 10.290 6.849

0.391

S. Burlington

Vermont 37202 7.136 9.842 0.170

Essex Junction

Vermont 37189 9.735 7.969

Effluent site

 tested ASI effluent ID Std. Dev (Colilert)

Std. Dev 

(SM9222D)

Bethel

Maine 37053 12.043 7.504

Northhampton

Massachusetts 37266 16.331 5.165

Winthrop

Massachusetts 37265 12.672 4.756

Keene

New Hampshire 37117 11.494 6.970

Littleton

New Hampshire 37435 14.506 8.262

W. Lebanon

New Hampshire 37118 9.388 6.253

Woodsville

New Hampshire 37365 11.178 6.849

Burlington

Vermont 37054 10.895 11.232

0.00

0.00

S. Burlington

Vermont 37202 8.201 9.842

Essex Junction

Vermont 37189 7.566 7.969

0.90

0.82

0.43

Table 15

F-test p-values for comparison of variances between Colilert and SM9222D

0.06

0.02

0.09

0.04

P-value

0.05

22
ATP Case No. N09-004 Study Report 

C-18 SM9222D

*

cfu or MPN per 100mL sample

Table 16

Fecal coliform recovery comparison (Colilert-18 vs. SM9222D)

0.000

0.623

7.200

-0.500

0.000

0.000

0.000

0.013

0.003

0.000

0.000

0.000

37189

58.0 46.0

58.4 40.8

65.8 39.6

37202

9.380

6.470

8.060

2.610

-3.230

4.990

6.370

-7.600

53.6

Essex Junction

Vermont

S. Burlington

Vermont

37053

37266

37265

37117

37435

37118

37365

37054

Littleton

New Hampshire

W. Lebanon

New Hampshire

Woodsville

New Hampshire

Burlington

Vermont

Bethel

Maine

Northhampton

Massachusetts

Winthrop

Massachusetts

Keene

New Hampshire

Effluent site

 tested ASI effluent ID T-value P-value Mean recovery*

27.9

40.5 23.6

58.0 37.1

50.9 43.5

37.2 44.6

52.1 31.9

42.0 43.4

CLT SM9222D

*

cfu or MPN per 100mL sample

S. Burlington

Vermont 37202 44.5 43.4 0.400 0.693

Essex Junction

Vermont 37189 67.7 31.9 14.600 0.000

Burlington

Vermont 37054 43.3 39.6 -1.060 0.295

Woodsville

New Hampshire 37365 55.2 40.8 4.900 0.000

W. Lebanon

New Hampshire 37118 65.8 46.0 7.880 0.000

Littleton

New Hampshire 37435 81.0 44.6 9.750 0.000

Keene

New Hampshire 37117 61.6 43.5 6.020 0.000

Winthrop

Massachusetts 37265 72.4 37.1 11.670 0.000

Northhampton

Massachusetts 37266 58.4 23.6 9.100 0.000

Bethel

Maine 37053 49.6 27.9 6.830 0.000

Table 17

Fecal coliform recovery comparison (Colilert vs. SM9222D)

Effluent site

 tested ASI effluent ID T-value P-value Mean recovery*

23
ATP Case No. N09-004 Study Report 

a

Two of the wells confirmed by EC broth were initially negative for growth. Bacterial samples obtained from frozen archive

b

False positive rate = confirmed false positive wells or colonies/total number of wells or colonies collected for confirmation

False positive rateb 0.5%

Table 18

Confirmation of Presumptive Positive Responses by Each Test Method

200 166 33 1

12 8 0

0

5 1

4 0

0

Total (all sites) 200 133

2

2

3

Colonies confirmed

(via. Vitek)

15

19 1

S. Burlington, VT 37202 20

19

15 5 0

Burlington, VT 37054 20

Essex Junction, VT 37189 20

1 0

Woodsville, NH 37365 20 14

West Lebanon, NH 37118 20 18

Keene, NH 37117 20 16

Littleton, NH 37435 20 18

0

Northhampton, MA 37266

Winthrop, MA 37265 20 17

20 17

False positive 

colonies

0 0

3 0

Bethel, ME 37053 20 20

Effluent site

 tested ASI effluent ID Colonies subjected to confirmation

Colonies confirmed 

(via. EC broth)

0

Fecal Coliform Positive Samples (SM9222D)

200 175 25 0

False positive rateb

Total (all sites)

S. Burlington, VT 20 5

0

Essex Junction, VT 37189 20 16 4 0

Burlington, VT 37054 20

3 0

West Lebanon, NH 37118

Woodsville, NH 37365 20 17

18

2 0

0 0

2 0

Littleton, NH 37435 20 20

Keene, NH 37117 20 18

2 0

Northhampton, MA 37266

Winthrop, MA 37265 20 18

18 2

Wells confirmed

(via. Vitek)

False positive 

wells

4 0

0

Bethel, ME 37053 20 16

Effluent site

 tested ASI effluent ID Wells subjected to confirmation

Wells confirmed 

(via. EC broth)

Fecal Coliform Positive Samples (Colilert Method)

Fecal Coliform Positive Samples (Colilert-18 Method)

False positive rateb 1.5%

5

7

1

0

45 3

1

0

0

0

0

0

14

13

1

0

4

9

6

3

3

4

1

3

17

16

19a

17

15

11

13

17

20

20

20

20

20

20

20

20

37435

Northhampton, MA

37189

S. Burlington, VT 37202

Essex Junction, VT

Woodsville, NH

Winthrop, MA

Littleton, NH

37053

37266

37265

37117

Total (all sites)

0.0%

37118

37365

37054

20

20

20

20

37202

False positive 

wells

Effluent site

 tested

Burlington, VT

West Lebanon, NH

Keene, NH

Wells subjected 

to confirmation ASI effluent ID Wells confirmed (via. EC broth)

Wells confirmed

(via. Vitek)

Bethel, ME

24
ATP Case No. N09-004 Study Report 

ASI 

effluent ID

Effluent site

 tested

ASI 

well ID VITEK ID - Isolate name VITEK ID

 confidence

307 Escherichia coli 99%

311 Escherichia coli 99%

312 Klebsiella pneumoniae ssp pneumoniae 97%

313 Escherichia coli 99%

622 Escherchia coli 99%

624 Enterobacter asburiae 96%

625 Klebsiella pneumoniae ssp pneumoniae 99%

627 Klebsiella pneumoniae ssp pneumoniae 96%

628 K. pneumoniae ssp pneumoniae & E. chloacae 99% & 98%

630 Klebsiella pneumoniae ssp pneumoniae 99%

636 Enterobacter cloacae 99%

637 K. pneumoniae ssp pneumoniae & E. chloacae 89% & 99%

639 Escherchia coli 99%

603 Raoultella planticola or Klebsiella pneumoniae N/A

611 Escherichia coli 99%

612 Klebsiella pneumoniae ssp pneumoniae 99%

614 K. pneumoniae ssp pneumoniae & E. chloacae 99%

615 Klebsiella pneumoniae ssp pneumoniae 99%

616 Klebsiella pneumoniae ssp pneumoniae 99%

617 Klebsiella pneumoniae ssp pneumoniae 95%

373 Klebsiella pneumoniae ssp pneumoniae 99%

374 Klebsiella pneumoniae ssp pneumoniae 97%

377 Escherichia coli 99%

766 Klebsiella pneumoniae or Klebsiella oxytoca N/A

767 Klebsiella pneumoniae ssp pneumoniae 91%

768 Klebsiella pneumoniae ssp pneumoniae 96%

382 Klebsiella pneumoniae ssp pneumoniae 94%

390 Klebsiella pneumoniae ssp pneumoniae 99%

394 Klebsiella pneumoniae ssp pneumoniae 99%

399 Klebsiella pneumoniae ssp pneumoniae 99%

37365 Woodsville

New Hampshire 711 Klebsiella pneumoniae ssp pneumoniae 96%

281 Klebsiella pneumoniae ssp pneumoniae 99%

291 Escherichia coli 99%

295 Escherichia coli 95%

484 Escherchia coli 99%

485 Enterbacter cloacae 99%

493 Klebsiella pneumoniae ssp pneumoniae 99%

497 K. pneumoniae ssp pneumoniae & E. coli 91% & 99%

499 Klebsiella pneumoniae ssp pneumoniae 99%

500 R. planticola or K. pneumoniae n/a

503 Escherichia coli 99%

507 Escherichia coli 99%

510 Escherichia coli 99%

515 Escherichia coli 99%

517 Klebsiella pneumoniae ssp pneumoniae 99%

518 Klebsiella pneumoniae ssp pneumoniae 99%

519 Escherichia coli 99%

 = false positive because Vitek ID was less than 90%

Bethel

Maine

37189

37202

37054

37118

Burlington

Vermont

W. Lebanon

New Hampshire

Essex Junction

Vermont

S. Burlington

Vermont

Table 19

Confirmation of presumptive positive wells by Vitek (Colilert method)

Winthrop

Massachusetts

Northampton

Massachusetts

Littleton

New Hampshire

Keene

New Hampshire

37266

37053

37265

37435

37117

25
ATP Case No. N09-004 Study Report 

ASI 

effluent ID

Effluent site

 tested

ASI 

well ID VITEK ID - Isolate name VITEK ID

 confidence

242 Escherichia coli 99%

248 Escherichia coli 99%

256 Escherichia coli 99%

257 Escherichia coli 99%

587 Klebsiella pneumoniae ssp pneumoniae 99%

600 Klebsiella pneumoniae ssp pneumoniae 99%

575 Klebsiella pneumoniae ssp pneumoniae 99%

580 Escherichia coli 99%

331 Klebsiella pneumoniae ssp pneumoniae 92%

337 Escherichia coli 99%

345 Klebsiella pneumoniae ssp pneumoniae 99%

358 Klebsiella pneumoniae ssp pneumoniae 99%

683 Klebsiella pneumoniae ssp pneumoniae 99%

689 Escherichia coli 99%

693 Escherichia coli 98%

37054 Burlington

Vermont 270 Escherichia coli 99%

442 Klebsiella pneumoniae ssp pneumoniae 99%

446 Klebsiella pneumoniae ssp pneumoniae 99%

447 Klebsiella pneumoniae ssp pneumoniae 99%

450 Escherichia coli 99%

461 Escherichia coli 99%

464 Enterbacter sakazakii 95%

469 Escherichia coli 99%

472 Escherichia coli & Klebsiella pneumoniae 99%

478 Escherichia coli 99%

Table 20

Confirmation of presumptive positive wells by Vitek (Colilert-18 method)

37266 Northampton

Massachusetts

37365 Woodsville

New Hampshire

37053 Bethel

Maine

37202 S. Burlington

Vermont

37265 Winthrop

Massachusetts

37118 W. Lebanon

New Hampshire

37189 Essex Junction

Vermont

37117 Keene

New Hampshire

26
ATP Case No. N09-004 Study Report 

ASI 

effluent ID

Effluent site

 tested

ASI 

colony ID VITEK ID - Isolate name VITEK ID

 confidence

670 Klebsiella pneumoniae ssp pneumoniae 99%

675 Klebsiella pneumoniae ssp pneumoniae 99%

679 Klebsiella pneumoniae ssp pneumoniae 99%

648 Klebsiella pneumoniae ssp pneumoniae 99%

655 Klebsiella pneumoniae ssp pneumoniae 99%

657 Klebsiella pneumoniae ssp pneumoniae 91%

402 Escherichia coli 99%

408 Klebsiella pneumoniae ssp pneumoniae 99%

410 Klebsiella pneumoniae ssp pneumoniae 99%

411 Escherichia coli 99%

786 Escherichia coli 93%

787 Escherichia coli 99%

432 Klebsiella pneumoniae ssp pneumoniae 94%

436 Klebsiella pneumoniae ssp pneumoniae 99%

725 Klebsiella pneumoniae ssp ozaenae 85%

727 Escherichia coli 99%

729 Escherichia coli 94%

733 Enterobacter sakazakii 91%

735 Escherichia coli 95%

738 Escherichia coli 95%

37054 Burlington

Vermont 147 Klebsiella pneumoniae ssp pneumoniae 99%

521 Escherichia coli 99%

523 Escherichia coli 99%

525 Escherichia coli 99%

527 Escherichia coli 98%

531 Escherichia coli 99%

542 Klebsiella pneumoniae ssp pneumoniae 99%

543 Klebsiella pneumoniae ssp pneumoniae 99%

546 Klebsiella pneumoniae ssp pneumoniae 99%

547 Klebsiella pneumoniae ssp pneumoniae 99%

553 Klebsiella pneumoniae ssp pneumoniae 99%

555 Klebsiella pneumoniae ssp pneumoniae 99%

559 Klebsiella pneumoniae ssp pneumoniae 99%

560 Escherichia coli 99%

 = false positive because Vitek ID was less than 90%

Table 21

Confirmation of presumptive positive colonies by Vitek (SM9222D)

Keene

New Hampshire

Winthrop

Massachusetts

S. Burlington

Vermont

Essex Junction

Vermont

W. Lebanon

New Hampshire

37435 Littleton

New Hampshire

Woodsville

New Hampshire

Northampton

Massachusetts

37202

37265

37266

37365

37117

37118

37189

27
ATP Case No. N09-004 Study Report 

SM9222D 1 Woodsville, NH Klebsiella pneumoniae ssp ozaenae 85%

Colilert-18 0 n/a n/a n/a

85%

n/a

Colilert 3 Wintrop, MA Raoultella planticola or Klebsiella pneumoniae 85%

Bethel, ME Non-viable bacterium

Essex Junction, VT Raoultella planticola or Klebsiella pneumoniae

Table 22

Fecal coliforms recovered from false positive wells or colonies

Test

 method

False positive 

responses

Effluent site Vitek ID Vitek confidence

28
ATP Case No. N09-004 Study Report 

a

False negative rate = confirmed false negative wells or colonies/total number of wells or colonies collected for confirmation

20 17

Table 23

Confirmation of Presumptive Negative Responses by Each Test Method

Fecal Coliform Negative Samples (Colilert Method)

Effluent site

 tested ASI effluent ID Wells subjected to confirmation

Wells confirmed 

(via. EC broth)

Wells confirmed

(via. Vitek)

False negative 

wells

0 3

Northhampton, MA 37266 20 19 0 1

Bethel, ME 37053

Winthrop, MA 37265 20 17

0 2

Keene, NH 37117 20 20

0 3

0 0

0 1

Littleton, NH 37435

West Lebanon, NH 37118 20 19

20 18

Woodsville, NH 37365 20 18

0 0

Burlington, VT 37054 20 15

0 2

0 5

0 1

Essex Junction, VT 37189

S. Burlington, VT 37202 20 19

20 20

Wells subjected 

to confirmation

Wells confirmed 

(via. EC broth)

18

False negative ratea 9.0%

Fecal Coliform Negative Samples (Colilert-18 Method)

Total (all sites) 200 182 0

Wells confirmed

(via. Vitek)

False negative 

wells

Bethel, ME 37053 20 20 0 0

Effluent site

 tested ASI effluent ID

Northhampton, MA 37266 20 19

0 1

Winthrop, MA 37265 20 19

0 1

0 1

0 2

Keene, NH 37117

Littleton, NH 37435 20 18

20 19

West Lebanon, NH 37118 20 15

0 1

Woodsville, NH 37365 20 18

0 5

0 2

0 0

Burlington, VT 37054

Essex Junction, VT 37189 20 20

20 19

S. Burlington, VT 37202 20 19

False negative 

colonies

0 1

Total (all sites) 200 186 0 14

20 9

False negative ratea 7.0%

Fecal Coliform Negative Samples (SM9222D)

Effluent site

 tested ASI effluent ID Colonies subjected to confirmation

Colonies confirmed 

(via. EC broth)

Colonies confirmed

(via. Vitek)

0 11

Northhampton, MA 37266 20 16 0 4

Bethel, ME 37053

Winthrop, MA 37265 20 16

0 3

Keene, NH 37117 20 18

0 4

0 2

0 5

Littleton, NH 37435

West Lebanon, NH 37118 20 15

36 33

Woodsville, NH 37365 17 11

0 3

Burlington, VT 37054 15 10

0 6

0 5

0 1

Essex Junction, VT 37189

S. Burlington, VT 37202 20 19

17 14

44

False negative ratea 21.5%

Total (all sites) 205 161 0

29
ATP Case No. N09-004 Study Report 

ASI 

effluent ID

Effluent site

 tested

ASI 

well ID VITEK ID - Isolate name VITEK ID

 confidence

306 Escherichia coli 99%

311 Escherichia coli 99%

319 Escherichia coli 98%

37266 Northampton

Massachusetts 622 Escherchia coli 94%

606 Escherichia coli 96%

607 Escherichia coli 88%

612 Escherichia coli 99%

767 Escherichia coli 99%

780 Escherichia coli 99%

37118 W. Lebanon

New Hampshire 389 Escherichia coli 96%

722 Klebsiella pneumoniae ssp pneumoniae 99%

740 Escherichia coli 99%

283 Escherichia coli 99%

287 Klebsiella pneumoniae ssp pneumoniae 99%

288 Escherichia coli & Enterobacter aerogenes 94% & 98%

289 Klebsiella pneumoniae ssp pneumoniae 95%

297 Klebsiella pneumoniae ssp pneumoniae 99%

37202 S. Burlington

Vermont 516 Escherichia coli 96%

Table 24

Confirmation of presumptive negative wells by Vitek (Colilert method)

37365 Woodsville

New Hampshire

37435 Littleton

New Hampshire

37265 Winthrop

Massachusetts

37053 Bethel

Maine

37054 Burlington

Vermont

30
ATP Case No. N09-004 Study Report 

ASI 

effluent ID

Effluent site

 tested

ASI 

well ID VITEK ID - Isolate name VITEK ID

 confidence

37266 Northampton

Massachusetts 597 Escherichia coli 99%

37265 Winthrop

Massachusetts 568 Klebsiella pneumoniae ssp pneumoniae 99%

37117 Keene

New Hampshire 335 Klebsiella pneumoniae ssp pneumoniae 99%

743 Escherichia coli 98%

750 Klebsiella pneumoniae ssp pneumoniae 99%

344 Klebsiella pneumoniae ssp pneumoniae 99%

347 Escherichia coli & Klebsiella pneumoniae 99% & 95%

348 Klebsiella pneumoniae ssp pneumoniae 99%

351 Klebsiella pneumoniae ssp pneumoniae 99%

359 Klebsiella pneumoniae ssp pneumoniae 99%

681 Klebsiella pneumoniae ssp pneumoniae 99%

698 Klebsiella pneumoniae ssp pneumoniae 99%

37054 Burlington

Vermont 268 Escherichia coli 99%

37202 S. Burlington

Vermont 461 Klebsiella pneumoniae ssp pneumoniae 94%

37435 Littleton

New Hampshire

Table 25

Confirmation of presumptive negative wells by Vitek (Colilert-18 method)

37365 Woodsville

New Hampshire

37118 W. Lebanon

New Hampshire

31
ATP Case No. N09-004 Study Report 

ASI 

effluent ID

Effluent site

 tested

ASI 

colony ID VITEK ID - Isolate name VITEK ID

 confidence

123 Escherichia coli 99%

124 Escherichia coli 99%

125 Escherichia coli 99%

126 Escherichia coli 99%

130 Escherichia coli 99%

132 Escherichia coli 99%

134 Escherichia coli 99%

135 Escherichia coli & Klebsiella pneumoniae 99%

136 Escherichia coli 99%

137 Escherichia coli 99%

138 Escherichia coli 99%

661 Klebsiella pneumoniae ssp pneumoniae 94%

669 Escherichia coli 99%

673 Escherichia coli 99%

674 Klebsiella pneumoniae ssp pneumoniae 99%

642 Klebsiella pneumoniae ssp pneumoniae 95%

643 Klebsiella pneumoniae ssp pneumoniae 99%

651 Klebsiella pneumoniae ssp pneumoniae 94%

655 Klebsiella pneumoniae ssp pneumoniae 95%

416 Escherichia coli & Klebsiella pneumoniae 99%

418 Escherichia coli 99%

790 Escherichia coli 99%

793 Escherichia coli 99%

795 Escherichia coli 99%

426 Escherichia coli 99%

430 Escherichia coli 99%

432 Escherichia coli 99%

435 Escherichia coli 99%

438 Escherichia coli 99%

721 Escherichia coli 99%

723 Klebsiella pneumoniae ssp pneumoniae 99%

726 Enterbacter sakazakii 99%

730 Escherichia coli 99%

733 Escherichia coli 99%

734 Escherichia coli 99%

142 Klebsiella pneumoniae ssp pneumoniae 94%

144 Klebsiella pneumoniae ssp pneumoniae 98%

153 Escherichia coli 98%

156 Escherichia coli 99%

158 Escherichia coli 99%

521 Escherichia coli 99%

529 Klebsiella pneumoniae ssp pneumoniae 99%

531 Escherichia coli 99%

37202 S. Burlington

Vermont 548 Escherichia coli 99%

Table 26

Confirmation of presumptive negative colonies by Vitek (SM9222D)

37189 Essex Junction

Vermont

37265 Winthrop

Massachusetts

37118 W. Lebanon

New Hampshire

37117

37435 Littleton

New Hampshire

Keene

New Hampshire

37054 Burlington

Vermont

37053 Bethel

Maine

37266 Northampton

Massachusetts

37365 Woodsville

New Hampshire

32
ATP Case No. N09-004 Study Report 

75%

2%

0%

SM9222D Klebsiella 23% 44

Escherichia

Enterobacter

Citrobacter

27%

0%

0%

Colilert-18 Klebsiella 71% 14

Escherichia

Enterobacter

Citrobacter

Enterobacter

Citrobacter

78%

0%

0%

Klebsiella 4 22%

0 Colilert 18

Test

 method

False negative 

responses

Genera Occurrence Frequency

Escherichia 14

0

Table 27

Fecal coliforms recovered from false negative wells or colonies

0

33

10

1

0

4

10

0

33
ATP Case No. N09-004 Study Report 

Figure 1 

Confirmation Procedure for Positive Response Samples 

 Colilert-18/Q-tray 2000

Colilert/Q-tray 2000

Method 9222D

Select 1 “positive response” 

from each replicate for 

verification

Frozen (-70oC) 

archived source

LTB incubated at 

35±0.5oC for 48±3 hrs

EC Broth incubated at 

44.5±0.2oC for 24±2 hrs

Gas & growth No Gas & growth

True Positive

Nutrient Agar slant

incubated at 35±

0.5oC for 18 to 24 hrs

VITEK

Fecal Coliform non-fecal coliform

True Positive Possible

False Positive McConkey agar

plate incubated at 35±

0.5oC for 18 to 24 hrs

10μL aliquot 

or colony

800μL from Colilert & 

Colilert-18 samples

800μL from 

9222D samples

10μL aliquot

10μL aliquot

10μL aliquot

Frozen (-70oC) 

archived source

False Positive

Has archived 

source been 

screened?

(2 cycles)

YES (after 2 rounds of testing)

NO

All typical and atypical coliform colony types

=90% confidence <90% confidence

EMB Agar

incubated at 35±

0.5oC for 18 to 24 hrs

Typical fecal coliform colony types

34
ATP Case No. N09-004 Study Report 

Figure 2 

Confirmation Procedure for Negative Response Samples 

Colilert-18/Q-tray 2000

Colilert/Q-tray 2000

Method 9222D

Select 1 “negative response” 

from each replicate for 

verification

Frozen (-70oC) 

archived source

LTB incubated at 

35±0.5oC for 48±3 hrs

EC Broth incubated at 

44.5±0.2oC for 24±2 hrs

Gas & growth No Gas & growth

False Negative

Nutrient Agar slant

incubated at 35±

0.5oC for 18 to 24 hrs

VITEK ID

10μL aliquot 

or colony

800μL from Colilert & 

Colilert-18 samples

800μL from 

9222D samples

10μL aliquot

True Negative

Turbid

Growth?

YES

NO

McConkey Agar

incubated at 35±

0.5oC for 18 to 24 hrs

10μL aliquot

Typical fecal coliform colony types

35
ATP Case No. N09-004 Study Report 

APPENDIX A(1) Method 

IDEXX Colilert® -18 TEST METHOD FOR THE DETECTION OF 

FECAL COLIFORMS IN WASTEWATER

36
ATP Case No. N09-004 Study Report 

APPENDIX A(1) Method 

1.0 Scope and Application 

1.1 This method is intended for use in the detection and enumeration of fecal 

coliform bacteria in wastewater samples without additional confirmation 

steps; any positive response (yellow wells) is an indication of the presence 

of fecal coliform bacteria 

1.2 The minimum, non-zero number of bacterial counts detectable with this 

method is a function of any dilution scheme used when processing the 

sample 

1.3 The Colilert-18® method, when used in conjunction with Quanti-Tray® or 

Quanti-Tray®/2000, is a quantification method capable of detecting up to 

2419MPN/100mL fecal coliforms without a dilution (see package insert) 

(1)

1.4 Since there can be wide range of fecal coliform populations in wastewater 

samples, dilutions can be used with this method for detecting and 

enumerating the actual concentration 

2.0 Summary of Method 

2.1 The method is based on Defined Substrate Technology®, when reagent is 

added to the wastewater sample and incubated, it can detect fecal 

coliform bacteria at 1 CFU/100mL within 18 hours with as many as 2 

million heterotrophic bacteria present per 100 mL 

2.2 The Colilert-18 tests are manufactured in snap packs; one snap pack 

contains enough powder to perform a single test for the presence of fecal 

coliform bacteria in a 100mL wastewater sample. Contents of one snap 

pack is added to a vessel containing 100mL of a wastewater sample 

followed by mixing (shaking) to dissolve the powder. The sample plus 

medium is then transferred to a sterile Quanti-Tray device then placed into 

the Quanti-Tray sealer, which disperses and seals the sample into a large 

number of incubating wells. The Quanti-Tray is then incubated at 44.5 

±0.2°C for 18 hours. After incubation, the number of yellow wells are 

counted and compared against a most probable number (MPN) chart 

(provided with the Quanti-Trays) to arrive at the final number of fecal 

coliform bacteria detected in the original wastewater sample. If a dilution 

is used, the MPN/100mL is corrected based on the dilution. If needed, the 

yellow wells can be compared against a color comparator-. to verify the 

result. Any yellow color present in the wells which meets or exceeds the 

color of the comparator is considered to be positive for the presence of 

fecal coliform bacteria. The presence of this yellow color under these 

incubation conditions is specific for the detection of fecal coliform bacteria. 

No additional confirmation steps are required. 

3.0 Definitions 

3.1 In this method, fecal coliform bacteria are those bacteria which produce a 

yellow color after incubation at 44.5°C±0.2oC for 18 hours, specifically 

37
ATP Case No. N09-004 Study Report 

APPENDIX A(1) Method 

those bacteria in Escherichia, Klebsiella, Enterobacter, or Citrobacter 

genus capable of growing at 44.5 ± 0.2oC 

3.2 Fecal coliform detection is based on the presence of the enzyme ß-Dgalactosidase which is known to be present in fecal coliform bacteria 

3.3 For the detection of ß-D-galactosidase, Colilert-18 utilizes the 

chromogenic nutrient indicator ortho-nitrophenyl-ß-D-galactopyranoside 

(ONPG), which produces a distinct yellow color when hydrolyzed by ß-Dgalactosidase 

 

4.0 Interferences 

4.1 Heterotrophic bacteria greater than 2,000,000/100ml can yield a positive 

response for the presence of fecal coliform bacteria (1) 

4.2 Samples containing humic material may have an innate color and a 

control blank of the same sample may be required for comparison to the 

inoculated sample (1)

5.0 Safety

5.1 The analyst/technician must know and observe the normal safety 

procedures required in a microbiology laboratory preparing, using, and 

disposing of samples, reagents and materials, and while operating 

sterilizing equipment. 

5.2 Any waste containing potentially viable microorganisms should be 

decontaminated by following specific State and/or Federal guidelines 

 

6.0 Equipment and Supplies 

6.1 Pipettes, sterile, T.D. bacteriological or Mohr, glass or plastic of 

appropriate volume 

6.2 Quanti-Tray or Quanti-Tray 2000 trays (IDEXX part numbers WQT100 and 

WQT-2K, respectively) 

6.3 Quanti-Tray Sealer (IDEXX part number WQTS2X-115)

6.4 Sterile vessels, glass or plastic, 120-250ml volume 

6.5 Incubator/Water Bath maintained at 44.5°C±0.2°C 

6.6 EZ QC (quality control bacterial strains) (IDEXX part number WEZQ)

7.0 Reagents

7.1 Sterile deionized water or distilled water; water conforming to specification 

D1193, “Reagent water conforming to Type II, Annual Book of ASTM 

Standards” (2); autoclave at 121°C (15 -lb pressure) for 15 minutes or 

sterile filter using a 0.22 micron filter into a sterile container 

7.2 Colilert-18 snap packs, store at 2°C-25°C away from light. The expiration 

date is indicated on the package (15 months from the date of 

manufacture). (1); IDEXX part numbers WP020I-18 or WP200I-18) 

7.3 Antifoam drops or vessels (IDEXX part number WAFDB) 

7.4 Sodium thiosulfate 

38
ATP Case No. N09-004 Study Report 

APPENDIX A(1) Method 

8.0 Sample Collection, Preservation and Storage 

8.1 Sampling procedures are described in detail in the USEPA microbiology 

methods manual, Section II, A (18.3)(3) Samples were collected in bottles 

containing preservatives as outlined in Standard Methods for the 

Examination of Water and Wastewater, 20th Edition, section 9060 A.2. (4)

8.2 Storage Temperature and Handling Conditions: Ice or refrigerate 

bacteriological samples at a temperature less than 10°C ( 2°C-10°C) 

during transit to the laboratory (3); use insulated containers to assure 

proper maintenance of storage temperature. Ensure that sample vessels 

are not totally immersed in water during transit 

8.3 Holding Time Limitations: Examine samples as soon as possible after 

collection. For wastewater compliance, do not exceed 6 hours holding 

time and process samples within 2 hours 

8.4 Chlorinated wastewater samples should be neutralized at time of 

collection with sodium thiosulfate 

9.0 Quality Control

9.1 Quality control should be conducted on each lot of Colilert-18 or more 

often as regulations requires. Inoculate 100 ml of sterile water with either 

EZ QC or ATCC strains as listed below. Follow the procedure in section 

11.0 and then refer to this chart for results interpretation: 

 

 EZ QC Bacteria Expected Result 

EZ QC Escherichia coli Yellow color production 

EZ QC Pseudomonas 

aeruginosa 

No color change 

10.0 Calibration and Standardization 

10.1 Check the temperature in the incubator daily to ensure it is within the 

stated limits 

10.2 Check thermometers at least annually against NIST certified thermometer 

or one that meets the requirements of NIST Monograph SP 250-23 

11.0 Procedure 

11.1 Carefully separate one blister pack from the strip taking care not to 

accidentally open the adjacent pack 

11.2 Ensure the powder is in the bottom of the blister pack 

11.3 Hold the blister pack face down (paper side up) at the top and towards the 

bottom and snap back at the scoreline forming a “V”, with the opening 

facing into the open vessel. 

11.4 Allow the powder to fall into the water sample contained in the sterile 

vessel 

39
ATP Case No. N09-004 Study Report 

APPENDIX A(1) Method 

11.5 If a dilution is required, use sterile deionized or distilled water, not buffered 

water for making the dilutions. Always add Colilert-18 to the final 100ml 

diluted sample only 

11.6 If foaming occurs add Antifoam solution to the sample containing the 

reagent (1); alternatively, allow the vessel to sit for several minutes prior to 

adding it to a Quanti-Tray 

11.7 Aseptically cap and seal the vessel 

11.8 Mix/shake gently to dissolve 

11.9 Remove either a sterile Quanti-Tray or Quanti-Tray/2000 tray from the 

plastic bag (tear open the plastic bag at the bottom which has a green line 

around the bag) and squeeze the tray at the top to open it; pour the 

sample reagent mixture from the vessel into the tray avoiding contact with 

the foil tab 

11.10 Seal the tray with the Quanti-Tray sealer 

11.11 Incubate at 44.5° ± 0.2°C 

11.12 Read the results at 18 hours. If needed, compare the yellow color of the 

wells with either a Quanti-Tray or Quanti-Tray2000 comparator

11.13 If no yellow color is observed in the wells, the sample is absence of fecal 

coliforms and recorded as <1/100mL 

11.14 If the sample has a yellow color equal to or greater than the comparator, 

the presence of fecal coliforms is confirmed

11.15 If the sample is yellow, but not distinguishable from the color comparator, 

it may be incubated up to an additional 4 hours (but no more than 22 

hours total). If the color is then equal to or greater than the comparator, 

the sample is positive for fecal coliforms; if it does not change, it is 

negative 

12.0 Data Analysis and Calculations 

12.1 Count the number of positive wells. 

12.2 Refer to the Quanti-Tray MPN table provided by IDEXX to determine the 

 most Probable Number (MPN) for total coliforms (yellow wells) (1)

12.3 Correct the MPN value for any dilution made. 

12.4 Report results as MPN/100mL for fecal coliforms. 

13.0 Method Performance 

13.1 The Colilert-18 method is a superior and more reliable test for the detection 

and enumeration of fecal coliform bacteria from wastewater than the 

SM9222D method as described in: ATP Study Report for the Proposed US 

EPA Approval of Detection and Enumeration of Fecal Coliforms in US 

Wastewater Samples (5)

14.0 Pollution Prevention 

14.1 The solutions and reagents used in this method pose no threat to the 

environment when recycled and managed properly. 

40
ATP Case No. N09-004 Study Report 

APPENDIX A(1) Method 

14.2 Solutions and reagents should be prepared in volumes consistent with 

laboratory use to minimize the volume of expired materials to be disposed. 

15.0 Waste Management 

15.1 It is the laboratory’s responsibility to comply with all federal, state and local 

regulations governing waste management, particularly the biohazard and 

hazardous identification rules and land disposal regulations. Compliance 

with all sewage discharge permits and regulations is also required. 

15.2 Samples, reference materials and equipment known or suspected to have 

viable bacteria attached or contained must be sterilized prior to disposal. 

16.0 References 

 1. Draft Colilert-18 Package Insert and Quanti-Tray and Quanti-Tray/2000 

Package Inserts from IDEXX 

 2. Annual Book of ASTM Standards, Vol. 11.01, American Society for 

Testing Materials, Philadelphia, PA 19103, (2005) 

 3. Bordner, R., J.A. Winter and P.V. Scarpino (eds.) Microbiological 

Methods for Monitoring the Environment, Water and Wastes, EPA-600/8-

78-017. Office of Research and Development, USEPA. (December 1978) 

 4. Clesceri, L.S., A.E. Greenberg, A.D. Eaton (eds.). 1998 Standard 

Methods for the examination of Water and Waster Water, 20th Edition, 

American Public Health Association, Washington, DC. 

 5. ATP Study Report for the Proposed US EPA Approval of Detection and 

Enumeration of Fecal Coliforms in US Wastewater Samples, January 

2010 

41
ATP Case No. N09-004 Study Report 

APPENDIX A(2) Method 

IDEXX Colilert® TEST METHOD FOR THE DETECTION 

OF FECAL COLIFORMS IN WASTEWATER 

42
ATP Case No. N09-004 Study Report 

APPENDIX A(2) Method 

1.0 Scope and Application 

1.1 This method is intended for use in the detection and enumeration of fecal 

coliform bacteria in wastewater samples without additional confirmation 

steps; any positive response (yellow wells) is an indication of the presence 

of fecal coliform bacteria 

1.5 The minimum, non-zero number of bacterial counts detectable with this 

method is a function of any dilution scheme used when processing the 

sample 

1.6 The Colilert® method, when used in conjunction with Quanti-Tray® or 

Quanti-Tray® /2000, is a quantification method capable of detecting up to 

2419MPN/100mL fecal coliforms without a dilution (see package insert) 

(1)

1.7 Since there can be wide range of fecal coliform populations in wastewater 

samples, dilutions can be used with this method for detecting and 

enumerating the actual concentration 

2.0 Summary of Method 

2.1 The method is based on Defined Substrate Technology®; when the 

reagent is added to the wastewater sample and incubated, it can detect 

fecal coliform bacteria at 1 CFU/100mL within 24 hours with as many as 2 

million heterotrophic bacteria present per 100 mL 

2.2 The Colilert tests are manufactured in snap packs; one snap pack 

contains enough powder to perform one test for the presence of fecal 

coliform bacteria in 100mL of wastewater. Add the contents of one snap 

pack to a vessel containing 100mL of a wastewater sample followed by 

mixing (shaking) to dissolve the powder. This is transferred to a sterile 

Quanti-Tray device and is then place into the Quanti-Tray sealer which 

dispenses and seals the reagent into a large number of incubating wells. 

The Quanti-Tray is then incubated at 44.5 ±0.2°C for 24 hours. After 

incubation, the number of yellow wells are counted and compared against 

a most probable number (MPN) chart (provided with the Quanti-Trays) to 

arrive at the final number of fecal coliform bacteria detected in the original 

wastewater sample. If a dilution is used, the MPN/100mL is corrected 

based on the dilution. If needed, the yellow wells can be compared against 

a color comparator-. to verify the result. Any yellow color present in the 

wells which meets or exceeds the color of the comparator is considered to 

be positive for the presence of fecal coliform bacteria. The presence of 

this yellow color under these incubation conditions is specific for the 

detection of fecal coliform bacteria. No additional confirmation steps are 

required. 

43
ATP Case No. N09-004 Study Report 

APPENDIX A(2) Method 

3.0 Definitions 

3.1 In this method, fecal coliform bacteria are those bacteria which produce a 

yellow color after incubation at 44.5°C±0.2°C for 24 hours, specifically 

those bacteria in Escherichia, Klebsiella, Enterobacter, or Citrobacter 

genus capable of growing at 44.5 ± 0.2oC 

3.2 Fecal coliform detection is based on the presence of the enzyme ß-Dgalactosidase which is known to be present in fecal coliform bacteria 

3.3 For the detection of ß-D-galactosidase, Colilert utilizes the chromogenic 

nutrient indicator ortho-nitrophenyl-ß-D-galactopyranoside (ONPG), which 

produces a distinct yellow color when hydrolyzed by ß-D-galactosidase 

 

4.0 Interferences 

4.1 Heterotrophic bacteria greater than 2,000,000/100mL can yield a positive 

response for the presence of fecal coliform bacteria (1) 

4.2 Samples containing humic material may have an innate color and a 

control blank of the same sample may be required for comparison to the 

inoculated sample (1)

5.0 Safety

5.1 The analyst/technician must know and observe the normal safety 

procedures required in a microbiology laboratory preparing, using, and 

disposing of samples, reagents and materials, and while operating 

sterilizing equipment 

5.2 Any waste containing potentially viable microorganisms should be 

decontaminated by following specific State and/or Federal guidelines 

6.0 Equipment and Supplies 

6.1 Pipettes, sterile, T.D. bacteriological or Mohr, glass or plastic of 

appropriate volume 

6.2 Quanti-Tray or Quanti-Tray 2000 trays (IDEXX part numbers WQT100 and 

WQT-2K, respectively) 

6.3 Quanti-Tray Sealer (IDEXX part number WQTS2X-115)

6.4 Sterile vessels, glass or plastic, 120-250mL volume 

6.5 Incubator/Water Bath maintained at 44.5°C±0.2°C 

6.6 EZ QC (quality control bacterial strains) (IDEXX part number WEZQ)

7.0 Reagents

7.1 Sterile deionized water or distilled water; water conforming to specification 

D1193, “Reagent water conforming to Type II, Annual Book of ASTM 

Standards” (2); autoclave at 121°C (15 -lb pressure) for 15 minutes or 

sterile filter using a 0.22 micron filter into a sterile container 

7.2 Colilert snap packs, store at 2°C-25°C away from light. The expiration 

date is indicated on the package (15 months from the date of 

manufacture). (1) (IDEXX part numbers WP020I or WP200I) 

44
ATP Case No. N09-004 Study Report 

APPENDIX A(2) Method 

7.3 Sodium thiosulfate 

8.0 Sample Collection, Preservation and Storage 

8.1 Sampling procedures are described in detail in the USEPA microbiology 

methods manual, Section II, A (18.3)(3) Samples were collected in bottles 

containing preservatives as outlined in Standard Methods for the 

Examination of Water and Wastewater, 20th Edition, section 9060 A.2. (4). 

8.2 Storage Temperature and Handling Conditions: Ice or refrigerate 

bacteriological samples at a temperature less than 10°C ( 2°C-10°C) 

during transit to the laboratory (3). Use insulated containers to assure 

proper maintenance of storage temperature. Ensure that sample vessels 

are not totally immersed in water during transit. 

8.3 Holding Time Limitations: Examine samples as soon as possible after 

collection. For wastewater compliance, do not exceed 6 hours holding 

time and process samples within 2 hours. 

8.4 Chlorinated wastewater samples should be neutralized at time of 

collection with sodium thiosulfate 

9.0 Quality Control

9.1 Quality control should be conducted on each lot of Colilert or more often 

as regulations requires. Inoculate 100 mL of sterile water with either EZ 

QC or ATCC strains as listed below. Follow the procedure in section 11.0 

and then refer to this chart for results interpretation: 

 

 EZ QC Bacteria Expected Result 

EZ QC Escherichia coli Yellow color production 

EZ QC Pseudomonas 

aeruginosa 

No color change 

11.0 Calibration and Standardization 

10.1 Check the temperature in the incubator daily to ensure it is within the 

stated limits. 

10.2 Check thermometers at least annually against NIST certified thermometer 

or one that meets the requirements of NIST Monograph SP 250-23. 

11.0 Procedure 

11.1 Carefully separate one blister pack from the strip taking care not to 

accidentally open the adjacent pack 

11.2 Ensure the powder is in the bottom of the blister pack 

11.3 Hold the blister pack face down (paper side up) at the top and towards the 

bottom and snap back at the scoreline forming a “V”, with the opening 

facing into the open vessel 

45
ATP Case No. N09-004 Study Report 

APPENDIX A(2) Method 

11.4 Allow the powder to fall into the water sample contained in the sterile 

vessel 

11.5 If a dilution is required, use sterile deionized or distilled water, not buffered 

water for making the dilutions. Always add Colilert to the final 100mL 

diluted sample only. 

11.6 Aseptically cap and seal the vessel 

11.7 Mix/shake gently to dissolve 

11.8 Remove either a sterile Quanti-Tray or Quanti-Tray/2000 tray from the 

plastic bag (tear open the plastic bag at the bottom which has a green line 

around the bag) and squeeze the tray at the top to open it; pour the 

sample reagent mixture from the vessel into the tray avoiding contact with 

the foil tab 

11.9 Seal the tray with the Quanti-Tray sealer 

11.10 Incubate at 44.5° ± 0.2°C 

11.11 Read the results at 24 hours. If needed, compare the yellow color of the 

wells with either a Quanti-Tray or Quanti-Tray/2000 comparator

11.12 If no yellow color is observed in the wells, the sample is absence of fecal 

coliforms and recorded as <1/100mL 

11.13 If the sample has a yellow color equal to or greater than the comparator, 

the presence of fecal coliforms is confirmed

11.14 If the sample is yellow, but not distinguishable form the color comparator, 

it may be incubated up to an additional 4 hours (but no more than 28 

hours total); if the color is then equal to or greater than the comparator, 

the sample is positive for fecal coliforms; if it does not change, it is 

negative 

12.0 Data Analysis and Calculations 

12.1 Count the number of positive wells 

12.2 Refer to the Quanti-Tray MPN table provided by IDEXX to determine the 

 most Probable Number (MPN) for total coliforms (yellow wells) (1)

12.3 Correct the MPN value for any dilution made 

12.4 Report results as MPN/100mL for fecal coliforms 

13.0 Method Performance 

13.1 The Colilert method is a superior and more reliable test for the detection 

and enumeration of fecal coliform bacteria from wastewater than the 

SM9222D method as described in: ATP Study Report for the Proposed 

US EPA Approval of Detection and Enumeration of Fecal Coliforms in US 

Wastewater Samples (5)

14.0 Pollution Prevention 

14.1 The solutions and reagents used in this method pose no threat to the 

environment when recycled and managed properly 

46
ATP Case No. N09-004 Study Report 

APPENDIX A(2) Method 

14.2 Solutions and reagents should be prepared in volumes consistent with 

laboratory use to minimize the volume of expired materials to be disposed 

15.0 Waste Management 

15.1 It is the laboratory’s responsibility to comply with all federal, state and local 

regulations governing waste management, particularly the biohazard and 

hazardous identification rules and land disposal regulations. Compliance 

with all sewage discharge permits and regulations is also required 

15.2 Samples, reference materials and equipment known or suspected to have 

viable bacteria attached or contained must be sterilized prior to disposal 

16.0 References 

 1. Draft Colilert Package Insert and Quanti-Tray and Quanti-Tray/2000 

package inserts from IDEXX 

 2. Annual Book of ASTM Standards, Vol. 11.01, American Society for 

Testing Materials, Philadelphia, PA 19103, (2005) 

 3. Bordner, R., J.A. Winter and P.V. Scarpino (eds.) Microbiological 

Methods for Monitoring the Environment, Water and Wastes, EPA-600/8-

78-017. Office of Research and Development, USEPA. (December 1978) 

 4. Clesceri, L.S., A.E. Greenberg, A.D. Eaton (eds.). 1998 Standard 

Methods for the examination of Water and Waster Water, 20th Edition, 

American Public Health Association, Washington, DC. 

 5. ATP Study Report for the Proposed US EPA Approval of Detection and 

Enumeration of Fecal Coliforms in US Wastewater Samples. January 

2010 

47
ATP Case No. N09-004 Study Report 

48

Appendix D 

Supporting References 

1. EPA Microbiological Alternate Test Procedure (ATP) Protocol for Drinking 

Water, Ambient Water, and Wastewater Monitoring Methods, Guidance; EPA 

821-B-03-004; Environmental Protection Agency, Office of Water, 

Washington, DC 20460 2004 

2. Code of Federal Regulations 40 CFR 136.3 

3. APHA. 1998. Standard Methods for the Examination of Water and 

Wastewater. 20th Edition. American Public Health Association. 1015 15th 

Street, NW, Washington, DC 20005. 
UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

WASHINGTON, D.C. 20460 

Patsy Root 

Regulatory Affairs Specialist 

IDEXX Laboratories, Inc. 

I IDEXX Drive 

Westbrook ME 04092 

Dear Ms. Root: 

AUG 0 3 2009 

The Engineering and Analytical Support Branch (EASB) has completed a 

technical review of the method validation study plan submitted in support of the 

following alternate test procedure (ATP) application. 

OFFICE OF 

WATER 

ATP Case No's. Analyte Study Plan 

(wastewater) 

N09-0004 Fecal coliform 

Revision 1.0, 7/21/2009 

Addendum 1.0, 7/31/2009 

We are pleased to inform you that in the judgement of our technical staff the proposed 

study plan is adequate for the A TP program. We look forward to reviewing the complete 

data package when it is available. Please feel free to contact us if you have any 

additional questions regarding these applications. 

Robin K. Oshiro, Ph.D 

Microbiologist 

Engineering and Analysis Division 

EASB 

Sincerely, 

;;i_{l Lemuel Walker ~ A TP Coordinator 

Engineering and Analysis Division 

EASB 

lntemet Address (URL) • http://www.epa.gov 

Recycled/Recyclable • Printed with Vegetable Oil Based Inks on 100% Postconsumer, Process Chlorine Free Recycled Paper 
EPA Case # N09-004 

Study Plan 

Study Protocol for the Proposed US EPA Approval of 

IDEXX’s Colilert®

-18 and Colilert® for the Detection and 

Enumeration of Fecal Coliforms in Wastewater Samples 

July 21, 2009 

IDEXX Laboratories, Inc. 

One Idexx Drive 

Westbrook, Maine 04092 

1
EPA Case # N09-004 

Method Description 

1.0 Scope and Application

1.1 Colilert®-18 and Colilert® are Defined Substrate® methods for the 

detection of fecal coliforms in wastewater samples 

 

1.2 The sample type for the study is primary effluent, from wastewater 

treatment plants 

1.3 Colilert-18 and Colilert used in combination with IDEXX’s Quanti-Tray®

and/or Quanti-Tray®/2000 MPN methods allow for detecting and 

enumerating fecal coliforms at 18 hours (hrs) for Colilert-18 and/or 24 hrs 

for Colilert after incubating at 44.5 ± 0.2°C without additional confirmation 

or verification steps 

1.4 The limit of detection of Colilert-18 and Colilert is 1 colony-forming unit 

(cfu) of fecal coliforms per 100 mL of sample 

2.0 Summary of Method

2.1 Colilert®-18 and Colilert® used in combination with IDEXX’s Quanti-Tray®

and/or Quanti-Tray®/2000 methods detect and enumerate fecal coliforms

in wastewater samples 

2.2 Colilert-18 and Colilert are based on the detection of β-D-galactosidase, 

which are indicative of the fecal coliform group when the samples are 

incubated at 44.5 ±0.2°C; as the target microbes grow, they will hydrolyze 

chromogenic nutrient indicator specific for β-D-galactosidase; 

antimicrobial agents are present in the test reagent to suppress the growth 

of most non-target microorganisms 

2.3 Colilert-18 and Colilert both contain the chromogenic substrates, orthonitrophenyl-β-D-galactopyranoside (ONPG); upon hydrolysis by the fecal 

coliform bacteria at 44.5 ±0.2°C, the ONPG in Colilert-18 and Colilert/or 

release a chromogenic compound, ortho-nitrophenol (ONP), which 

changes the color of the test from colorless to yellow 

2.4 A blister pack of the reagent (Colilert-18 or Colilert) powder is added to a 

sterile test vessel containing 100 mL of a wastewater sample; after the 

powder is added, the vessel is shaken to dissolve the powder; the 

dissolved sample/medium mixture is added to a Quanti-Tray or QuantiTray/2000 and sealed with a Quanti-Tray sealer; the tray is then placed 

2
EPA Case # N09-004 

into an incubator at the specific temperature and time required for the 

specific test as indicated in 1.3 above 

3.0 Definitions 

3.1 ONPG (ortho-nitrophenyl-β-D-galactopyranoside) – A nutrient indicator 

containing a food source attached to a chromogen. The fecal coliform 

bacteria contain an enzyme, β-D-galactosidase that cleaves the food 

source, galactopyranoside and releases the chromogen, ONP, to yield a 

yellow color 

3.2 Hydrolysis – Biochemical reaction catalyzed by a bacterial enzyme 

resulting in the decomposition of a substrate by the addition of a water 

molecule 

3.3 Fecal Coliforms – A bacterial subset belonging to the Enterobacteriaciae 

family and defined biochemically by the presence of the enzyme β-Dgalactosidase and will grow at 44.5 ±0.2°C in both Colilert and Colilert-18 

reagent 

3.4 Quanti-Tray® – An automated MPN method that is used with IDEXX’s 

Colilert and Colilert-18 tests for the enumeration of specific target bacteria 

with a counting range up to 200 cfu/100 mL 

3.5 Quanti-Tray®/2000 – An automated MPN method that is used with 

IDEXX’s Colilert and Colilert-18 tests for the enumeration of specific target 

bacteria with a counting range up to 2419 cfu/100 mL 

4.0 Interferences 

4.1 There are no known interferences contained in wastewater which interfere 

with the performance of these test methods 

5.0 Safety 

5.1 The analyst must practice normal safety procedures for working in a 

microbiological laboratory and handling potentially bio-hazardous cultures 

5.2 Solid and liquid waste containing viable microorganisms should be 

decontaminated by following specific State and/or Federal guidelines 

5.3 Colilert®-18 and Colilert® are not listed as a suspected carcinogen. Refer 

to the Material Safety Data Sheet (MSDS) provided with the product for 

specific information relating to the potential hazards of this product by 

accidental ingestion or exposure 

3
EPA Case # N09-004 

6.0 Quality Assurance/Quality Control 

6.1 Quality Assurance: The laboratories participating in the study have 

comprehensive quality assurance programs in place. The laboratories are 

certified for microbiological analysis of water and wastewater by both their 

respective state laboratory certification programs and by the United States 

Environmental Protection Agency 

 

6.2 Quality Control: All QC procedures should be performed as specified in 

the methods. The study report will include sufficient raw data so that an 

independent reviewer can verify each determination and calculation 

performed by the laboratory or the study coordinator. This verification 

consists of tracing all steps of the method to the final result reported. The 

raw data are, generally, method-specific and may include but are not 

limited to the following: 

6.2.1 Sample numbers or other identifiers used by the laboratory 

6.2.2 Sample collection dates and times 

6.2.3 Verification that method-specified QC procedures were met for test 

samples and all associated QC samples 

6.2.4 Analysis dates and times for all steps in the method 

6.2.5 Sample volume 

6.2.6 Any measurements of ancillary parameters (i.e., temperature, pH, 

turbidity, percent solids, etc.) 

6.2.7 Results for all intermediate steps in the method 

6.2.8 Preliminary data steps to determine the final result 

6.2.9 Final result 

6.2.10 If appropriate, quantitation reports, data system outputs, and other 

data to link the raw data to the results reported 

6.2.11 Laboratory bench sheets and copies of all pertinent logbook pages 

for all sample preparation and cleanup steps, and for all other parts 

of the determination 

6.2.12 Temperature logs for water bath, incubators, refrigerators, etc. 

6.2.13 Media preparation information 

6.2.14 If appropriate, direct instrument readouts and other data to support 

the final results 

7.0 Equipment and Supplies: (not exhaustive) 

7.1 Equipment: 

7.1.1 Air incubators capable of maintaining temperatures at 35±0.5°C 

4
EPA Case # N09-004 

7.1.2 Air incubators capable of maintaining temperatures at 44.5±0.2°C 

(see Appendix A, incubator specification) 

7.1.3 Water bath maintained at 50°C for tempering agar 

7.1.4 Water bath capable of maintaining temperatures at 44.5±0.2oC 

7.1.5 IDEXX Quanti-Tray® Sealer 

7.1.6 VITEK® automated bacteriological identification system, to be used 

to resolve discrepant results obtained from confirmation procedures 

for both positive and negative responses 

7.1.7 All equipment required for Standard Method 9222D 

7.2 Glassware and supplies – all glassware and plastic vessels used for 

determining the presence of the bacteria species should be handled 

according to recommended guidelines and in keeping with good laboratory 

practices; all glassware and containers used in this study should be 

washed with a suitable laboratory detergent, rinsed thoroughly with tap 

water followed by rinsing with reagent water to remove residual detergent, 

and sterilized by autoclaving at 121°C for 15 minutes prior to use 

7.2.1 IDEXX sterile plastic 120 mL collection vessels w/o sodium 

thiosulfate 

7.2.2 Colilert®-18 and Colilert® tests pre-packaged in disposable blister 

packs 

7.2.3 Disposable sterile pipettes (1, 5, 10, 25, and 50mL) 

7.2.4 Calibrated Pipetteman and sterile disposable tips 

7.2.5 IDEXX Quanti-Tray® and/or Quanti-Tray®/2000 devices 

7.2.6 Antifoam for use with Colilert-18 

7.2.7 All materials required for the Standard Method 9222D 

8.0 Comparability & Specificity Study for the Detection and Enumeration of 

Fecal Coliforms in Wastewater Samples 

8.1 The study will be performed following the general procedure described in 

the Appendix of the EPA Microbiological Alternate Test Procedure (ATP) 

Protocol for Drinking Water, Ambient Water, and Wastewater Monitoring 

Methods. Guidance – April 2004

8.2 The study will be conducted at Analytical Services, Inc. (130 Allen Brook 

Lane, Williston, Vermont), a certified reference laboratory 

8.3 Comparability Experimental Set-Up Outline (Figure 1) 

8.3.1 At least ten primary sewage effluent wastewater samples known to 

contain fecal coliforms will be collected from multiple States within 

and surrounding the greater New England region. Samples will be 

collected in bottles containing preservatives as outlined in the 20th 

5
EPA Case # N09-004 

Ed. of Standard Method, 9060 A.2. Samples will be couriered to 

the laboratory in coolers packed with ice or “blue ice”. Samples will 

be analyzed within 6 hours of collection. Chemical characterization 

of the wastewater samples is not required and will not be performed 

8.3.2 The fecal coliform concentration in each wastewater sample will be 

estimated using the Colilert®-18/Quanti-Tray®/2000, 

Colilert®/Quanti-Tray®/2000 methods, and Standard Method 

9222D (m-FC medium) 

 

8.3.3 The wastewater sample will be serially diluted in sterile de-ionized 

water to obtain multiple (3 to 5) dilution sets of samples for the 

comparability testing in order to generate at least one set of 20 

replicates with approximately 20-50 cfu of fecal coliforms per 100 

mL of test sample 

8.3.4 One hundred milliliter aliquots of the diluted wastewater samples 

will be tested with the Colilert-18/Quanti-Tray/2000 method (in 

replicates of 20 for each dilution) following manufacturer’s 

instructions 

8.3.5 One hundred milliliter aliquots of the diluted wastewater samples 

will be tested with Colilert/Quanti-Tray/2000 method (in replicates of 

20 for each dilution) following manufacturer’s instructions 

8.3.6 One hundred milliliter aliquots of the diluted wastewater samples 

will be tested with the m-FC medium (in replicates of 20 for each 

dilution) following Standard Method 9222D instruction 

8.3.7 Colilert and Colilert-18 will be incubated in a dry 44.5±0.2oC 

incubator that has been documented to hold the 44.5oC 

temperature within 0.2oC throughout the chamber, over a 24 hour 

period as described in Standard Methods for the Examination of 

Water and Wastewater, 21st Ed, 2005, page 9-66 (Appendix A); the 

m-FC standard method will be incubated in a 44.5±0.2oC water 

bath in accordance with the standard method procedure 

8.3.8 The Colilert/Quanti-Tray/2000 method will be incubated for 24 

hours 

8.3.9 The Colilert-18/Quanti-Tray/2000 method will be incubated for 18 

hours 

8.3.10 The Standard Method 9222D samples will be incubated for 24±2 

hours 

6
EPA Case # N09-004 

8.3.11 After incubation, each test method will be read to determine the 

number of presumptive positive responses (yellow wells in the case 

of Colilert & Colilert-18 and blue colonies in the case of Standard 

Method 9222D 

8.3.12 One of the dilution sets in which Standard Method 9222D yields 

approximately 20 to 50 presumptive positive colonies will be 

retained for further analysis of the false positive and false negative 

error for fecal coliform detection 

8.3.13 An assessment of normality (verification of normal distribution of 

results) will be done for all test methods with statistical tests as 

specified by the EPA Microbiological Alternate Test Procedure 

(ATP) Protocol for Drinking Water, Ambient Water, and Wastewater 

Monitoring Methods, Guidance (page 8.5) 

8.3.14 An assessment of precision will be done for all test methods as 

specified by the EPA Microbiological Alternate Test Procedure 

(ATP) Protocol for Drinking Water, Ambient Water, and Wastewater 

Monitoring Methods, Guidance (page 8.5 – 8.6) 

8.3.15 Fecal coliform recovery by each method will be evaluated as 

specified by the EPA Microbiological Alternate Test Procedure 

(ATP) Protocol for Drinking Water, Ambient Water, and Wastewater 

Monitoring Methods, Guidance (page 8.8 – 8.9) 

8.3.16 Presumptive positive and presumptive negative responses from 

each of the test methods at the chosen dilution will be subjected to 

a further verification study to arrive at the relative false positive and 

false negative rates 

 

8.4 Determination of the False Positive Error for Fecal Coliform

Detection (Figure 2) 

8.4.1 Identify one presumptive positive (yellow) well from each Colilert 

and Colilert-18/Quanti-Tray/2000 replicate for further verification 

(20 samples from each method per dilution) using the following 

method 

 

8.4.1.1 Use an alcohol wipe to disinfect the paper/foil backing of 

the “presumptive positive” well identified on the QuantiTray/2000 device 

8.4.1.2 Use a sterile pipette tip to poke through the back of the 

well, extract 800 μL of sample and dispense into a cryo7
EPA Case # N09-004 

vial containing 200 μL of sterile glycerol. Label and store 

the vial in a -70oC freezer as a sample archive 

8.4.1.3 Using a new, sterile pipette tip, extract another 10 μL 

aliquot and transfer into Lauryl Tryptose Broth for fecal 

coliform confirmation following Standard Method

9221B.1b 

8.4.1.4 The Lauryl Tryptose Broth is incubated at 35±0.5 °C for 

48±3 hours (as per Standard Method 9221B.1) 

8.4.1.5 Transfer a 10 μL aliquot into EC Broth (as per Standard 

Method 9221E) and incubate in a 44.5±0.2oC water bath 

for 24±2 hours 

8.4.1.6 The presence of gas and turbid growth in the EC Broth is 

a confirmed positive response for a fecal coliform 

8.4.1.7 The absence of gas is considered a negative response for 

the presence of a fecal coliform 

8.4.1.8 Streak 10 μL aliquots from all non-gassing samples onto 

EMB agar plates and incubate at 35±0.5oC for 18 to 24 

hours 

8.4.1.9 Streak typical fecal coliform colony types from EMB plate 

onto Nutrient Agar slants/plates and incubate at 35±0.5oC 

for 18 to 24 hours 

8.4.1.10 Confirm bacterial identification from each Nutrient agar 

slants/plates using a VITEK Bacterial ID system 

8.4.1.11 A VITEK identification of greater than 90% confidence is 

considered a confirmed positive response for the 

detection of a fecal coliform 

8.4.2 Identify one presumptive positive (blue) colony from each Standard 

Method 9222D replicate for further verification (20 samples from 

each method per dilution) using the following method 

8.4.2.1 Inoculate these colonies into Lauryl Tryptose Broth for 

fecal coliform confirmation following Standard Method

9221B.1b 

8.4.2.2 The Lauryl Tryptose Broth is incubated at 35±0.5 °C for 

48±3 hours (as per Standard Method 9221B.1) 

8
EPA Case # N09-004 

8.4.2.3 Transfer 800 μL of sample into a cryo-vial containing 200 

μL of sterile glycerol. Label and store the vial in a -70oC 

freezer as a sample archive 

8.4.2.4 Transfer another 10 μL aliquot into EC Broth (as per 

Standard Method 9221E) and incubate in a 44.5±0.2oC 

water bath for 24±2 hours 

8.4.2.5 The presence of gas and turbid growth in the EC Broth is 

a confirmed positive response for a fecal coliform 

8.4.2.6 The absence of gas is considered a negative response for 

the presence of a fecal coliform 

8.4.2.7 Streak 10 μL aliquots from all non-gassing samples onto 

EMB agar plates and incubate at 35±0.5oC for 18 to 24 

hours 

8.4.2.8 Streak typical fecal coliform colony types from EMB plate 

onto Nutrient Agar slants/plates and incubate at 35±0.5oC 

for 18 to 24 hours 

8.4.2.9 Confirm bacterial identification from each Nutrient agar 

slants/plates using a VITEK Bacterial ID system 

8.4.2.10 A VITEK identification of greater than 90% confidence is 

considered a confirmed positive response for the 

detection of a fecal coliform 

8.4.3 If the presence of a fecal coliform is confirmed in EC Broth or by the 

VITEK ID system, the result is interpreted as a True Positive 

Response for the presence of a fecal coliform by the test method 

(i.e. Colilert, Colilert-18, or Standard Method 9222D). 

8.4.4 If a negative response for the presence of a fecal coliform is 

obtained, test the frozen archived sample using the following 

method 

8.4.4.1 Streak 10μL of frozen sample onto a MacConkey agar 

plate and incubate for 18 to 24 hours in a 35±0.5 oC 

incubator 

8.4.4.2 Inoculate all “typical” and “atypical” coliform colony types 

into EC Broth and incubate in a 44.5±0.2oC water bath for 

24±2 hours 

9
EPA Case # N09-004 

8.4.4.3 The presence of gas and turbid growth in the EC Broth is 

considered a confirmed positive response for the 

presence of a fecal coliform 

8.4.4.4 If none of the turbid tubes contain gas streak 10 μL 

aliquots onto EMB agar plates and incubate at 35±0.5oC 

for 18 to 24 hours 

8.4.4.5 Streak all colony types from EMB plates onto nutrient agar 

plates/slants and incubate at 35±0.5oC for 18 to 24 hours 

8.4.4.6 Confirm bacterial identification from each Nutrient agar 

slants/plates using a VITEK Bacterial ID system 

8.4.4.7 A VITEK identification of greater than 90% confidence is 

considered a confirmed positive response for the 

detection of a fecal coliform bacterium 

8.4.5 If the presence of a fecal coliform is confirmed in EC Broth or by the 

VITEK method, the result is interpreted as a True Positive 

Response for the presence of a fecal coliform by the test method 

(i.e. Colilert, Colilert-18, or Standard Method 9222D) 

8.4.6 Failure to confirm the presence of a fecal coliform by 2 cycles of the 

method described in section 8.4.3 is interpreted as a False 

Positive Response for fecal coliform by the test method 

8.4.7 This assay will be repeated until the validity of at least 200 positive 

responses have been tested from all three fecal coliform methods 

 

8.5 Determination of the False Negative Error for Fecal Coliform

Detection (Figure 3) 

8.5.1 Identify one presumptive negative (colorless) well from each Colilert 

and Colilert-18/Quanti-Tray/2000 replicate for further verification 

(20 samples from each method per dilution) using the following 

method 

 

8.5.1.1 Use an alcohol wipe to disinfect the paper/foil backing of 

the “presumptive negative” well identified on the QuantiTray/2000 device 

8.5.1.2 Use a sterile pipette tip to poke through the back of the 

well, extract 800 μL of sample and dispense into a cryo10
EPA Case # N09-004 

vial containing 200 μL of sterile glycerol. Label and store 

the vial in a -70oC freezer as a sample archive 

8.5.1.3 Using a new, sterile pipette tip, extract another 10 μL 

aliquot and transfer into Lauryl Tryptose Broth for fecal 

coliform confirmation following Standard Method

9221B.1b 

8.5.1.4 The Lauryl Tryptose Broth is incubated at 35±0.5 °C for 

48±3 hours (as per Standard Method 9221B.1) 

8.5.1.5 The absence of turbid growth is considered a confirmed 

negative response for the presence of a fecal coliform 

8.5.1.6 If turbid growth is present, transfer a 10 μL aliquot into EC 

Broth (as per Standard Method 9221E) and incubate in a 

44.5±0.2oC water bath for 24±2 hours 

8.5.1.7 The presence of gas and turbid growth in the EC Broth is 

considered a confirmed positive response for a fecal 

coliform 

8.5.1.8 The absence of gas is considered a confirmed negative 

response for the presence of a fecal coliform 

8.5.2 Identify one presumptive negative (grey or cream colored) colony 

from each Standard Method 9222D replicate for further verification 

(20 samples from each method per dilution) using the following 

method 

8.5.2.1 Inoculate these colonies into Lauryl Tryptose Broth for 

fecal coliform confirmation following Standard Methods

9221B.1b 

8.5.2.2 The Lauryl Tryptose Broth is incubated at 35±0.5 °C for 

48±3 hours (as per Standard Method 9221B.1) 

8.5.2.3 The absence of turbid growth is considered a confirmed 

negative response for the presence of a fecal coliform 

8.5.2.4 If turbid growth is present, transfer 800 μL of sample into 

a cryo-vial containing 200 μL of sterile glycerol. Label and 

store the vial in a -70oC freezer as a sample archive 

11
EPA Case # N09-004 

8.5.2.5 Transfer another 10 μL aliquot into EC Broth (as per 

Standard Method 9221E) and incubate in a 44.5±0.2oC 

water bath for 24±2 hours 

8.5.2.6 The presence of gas and turbid growth in the EC Broth is 

a confirmed positive response for a fecal coliform 

8.5.2.7 The absence of gas is considered a confirmed negative 

response for the presence of a fecal coliform 

8.5.3 If a confirmed negative response for the presence of a fecal 

coliform is obtained, the result is interpreted as a True Negative 

Response for the presence of a fecal coliform by the test method 

8.5.4 If a confirmed positive response for the presence of a fecal coliform 

is obtained, the result is interpreted as a False Negative 

Response for the presence of a fecal coliform by the test method 

8.5.5 Streak all confirmed positive samples from the EC Broth onto 

MacConkey agar plates and incubate at 35±0.5oC for 18 to 24 

hours 

8.5.6 Transfer all typical fecal coliform colony types onto Nutrient Agar 

slants/plates and incubate at 35±0.5oC for 18 to 24 hours 

 

8.5.7 Confirm the bacterial identification from all the Nutrient agar slants 

using a VITEK Bacterial ID system 

8.5.8 This assay will be repeated until the validity of at least 200 negative 

responses have been tested for all three test methods 

8.6 False Positive and False Negative Rate Calculation

8.6.1 False Positive Rate = FP/(TP) X 100% 

FP = False Positives, which are the number of initial positive 

responses by the method which are not verified by the confirmation 

procedure 

TP = Total Positives, which are the number of total positive 

responses (both confirmed and non-confirmed positive samples) by 

the test method 

8.6.2 False Negative Rate = FN/(TN) X 100% 

12
EPA Case # N09-004 

FN = False Negatives, which are the number of initial negative 

responses by the method which are verified by the confirmation 

procedures to contain true positive samples 

TN = Total Negatives, which are the number of total negative 

responses (both confirmed and non-confirmed negative samples) 

by the test method 

 

13
EPA Case # N09-004 

Figure 1. Overall Experimental Flow Chart 

 

 

Sewage Effluent Samples

Analyzed within 30 hrs of collection and 

4 hrs of arrival at the laboratories

Prepare multiple dilution samples in sterile 

de-ionized water

Test Methods

Colilert-18/Quanti-Tray 2000 and 

Colilert/Quanti-Tray 2000

3-5 Dilutions and each dilution with 20 

replicates for each method

Method 9222D (m-FC)

3-5 Dilutions and each 

dilution with 20 replicates

Add Colilert-18 and/or Colilert reagent 

to 100mL diluted wastewater sample, 

allow the reagent to dissolve and pour 

the reagent/sample mixture in a 

Quanti-Tray 2000 subsequently sealed 

by the Quanti-Tray Sealer

Filter 100mL aliquot of 

diluted wastewater sample 

through a membrane and 

place the membrane onto 

the mFC medium.

Incubate at 44.5 ± 0.5oC for 18 hrs for 

the Colilert-18/Q-Tray 2000 method & 

24 hrs Colilert/Q-Tray method

Incubate the mFC medium at 

44.5 ± 0.2°C for 24 +/- 2 hrs

Count the number of wells with positive 

response (yellow color) for Colilert-18 

and Colilert) for fecal coliforms. 

Convert to MPN/100mL using IDEXX 

MPN Table

Count the number of blue 

colonies as presumptive 

fecal coliforms

14
EPA Case # N09-004 

 Figure 2. Confirmation Procedure for Positive Response Samples 

Colilert-18/Q -tray 2000

Colilert/Q -tray 2000

M ethod 9222D

Select 1 “positive response” 

from each replicate for 

verification

Frozen (-70oC) 

archived source

LTB incubated at 

35±0.5oC for 48±3 hrs

E C B roth incubated at 

44.5±0.2oC for 24±2 hrs

G as & growth N o G as & growth

True Positive

N utrient A gar slant

incubated at 35±

0.5oC for 18 to 24 hrs

VITEK

Fecal C oliform non-fecal coliform

True Positive P ossible

False Positive McConkey agar

plate incubated at 35±

0.5oC for 18 to 24 hrs

10μL aliquot 

or colony

800μL from C olilert & 

C olilert-18 sam ples

800μL from 

9222D sam ples

10μL aliquot

10μL aliquot

10μL aliquot

Frozen (-70oC) 

archived source

False Positive

H as archived 

source been 

screened?

(2 cycles)

YE S (after 2 rounds of testing)

N O

All typical and atypical coliform colony types

=90% confidence <90% confidence

EMB Agar

incubated at 35±

0.5oC for 18 to 24 hrs

T ypical fecal coliform colony types

 

15
EPA Case # N09-004 

16

 Figure 3. Confirmation Procedure for Negative Response Samples 

Colilert-18/Q-tray 2000

Colilert/Q-tray 2000

Method 9222D

Select 1 “negative response” 

from each replicate for 

verification

Frozen (-70oC) 

archived source

LTB incubated at 

35±0.5oC for 48±3 hrs

EC Broth incubated at 

44.5±0.2oC for 24±2 hrs

Gas & growth No Gas & growth

False Negative

Nutrient Agar slant

incubated at 35±

0.5oC for 18 to 24 hrs

VITEK ID

10μL aliquot 

or colony

800μL from Colilert & 

Colilert-18 samples

800μL from 

9222D samples

10μL aliquot

True Negative

Turbid

Growth?

YES

NO

McConkey Agar

incubated at 35±

0.5oC for 18 to 24 hrs

10μL aliquot

Typical fecal coliform colony types
Addendum to ATP Case # N09-004 

IDEXX Laboratories, Inc. 

July 31, 2009 

EPA Question #1 -"definition of fecal coliform as defined by your method, to wit, 

Colilert/18 Quanti-Tray/2000 positive result when run not at the current 

instruction specification of 35C but at 44.5C (with the plus minus degrees)" 

IDEXX response - A fecal coliform is defined as a subset of the total coliform 

population capable of being detected by the Colilert/Colilert-18 technology after 

incubation at 44.5 ±0.2C as indicated by the presence of a yellow well in the 

Quanti-Tray/Quanti-Tray 2000 test device. 

EPA Question #2- "verification via VITEK, where an identification of greater 

than 90% confidence of the following genera xxx will be considered a confirmed 

response for the detection of fecal coliform" 

IDEXX response: "verification via VITEK, where an identification of greater than 

90% confidence, with either SM 92220 or IDEXX method, of the following 

genera: Escherichia, Citrobacter, Klebsiella, and Enterobacter, will be considered 

a confirmed response for the detection of fecal coliform" 

P~t5Y)R~, 

0;;~~------ Regulatory Affairs Specialist 

IDEXX Laboratories, Inc. 

tel 1-207-556-894 7 

fax 1-207-556-4865 

mobile 207-523-0835 

patsy-root@idexx.com 

idexx.com 
06-XXXXX-00

Colilert®-18 

DRAFT

Colilert®

-18 Test Kit

Introduction

Colilert-18 either simultaneously detects total coliforms and E. coli or fecal 

coliforms in water. It is based on IDEXX’s patented Defined Substrate Technology®

(DST®). When total/fecal coliforms metabolize Colilert-18’s nutrient-indicator, 

ONPG, the sample turns yellow. When E. coli metabolize Colilert-18’s nutrientindicator, MUG, the sample fluoresces. Colilert-18 can detect these bacteria at 1 

cfu/100 ml within 18 hours even with as many as 2 million heterotrophic bacteria 

per 100 ml present.

Contents

• WP020I-18 contains 20 Snap Packs for 100 mL samples

• WP200I-18 contains 200 Snap Packs for 100 mL samples

Storage

Store at 2°C–25°C away from light.

Presence/Absence (P/A) Procedure

1. Add contents of one pack to a 100 mL sample in a sterile, transparent, 

nonfluorescing vessel.

2. Cap vessel and shake.

3. If sample is not already at 33°C–38°C, then place vessel in a 35°C waterbath for 

20 minutes or, alternatively, a 44.5°C waterbath for 7–10 minutes.

4. Incubate at 35°C ±0.5°C for the remainder of the 18 hours.

5. Read results according to Result Interpretation table below.

Quanti-Tray® Enumeration Procedure

1. Add contents of one 

pack to a 100 mL room 

temperature water sample 

in a sterile vessel. 

2. Cap vessel and shake 

until dissolved.

3. Pour sample/reagent mixture into a 

Quanti-Tray or Quanti-Tray/2000 and 

seal in an IDEXX Quanti-Tray Sealer.
4. Place the sealed tray in a 35°C ±0.5°C (or 44.5°C ±0.2°C for fecal coliforms) 

incubator for 18 hours (prewarming is not required). 

5. Read results according to the Result Interpretation table below. Count the

number of positive wells and refer to the MPN table provided with the trays to 

obtain a Most Probable Number.

Result Interpretation

 Appearance Result

Less yellow than 

the comparator*

Negative for total coliforms, 

E. coli and fecal coliforms

Yellow equal to or greater than 

the comparator* when incubated 

at 35°C ±0.5°C

Positive for total coliforms

Yellow and fluorescence equal to 

or greater than the comparator*

when incubated at 35°C ±0.5°C

Positive for E. coli

Yellow equal to or greater than 

the comparator* when incubated 

at 44.5°C ±0.2°C

Positive for fecal coliforms

• Look for fluorescence with a 6-watt, 365-nm UV light within 5 inches of the 

sample in a dark environment. Face light away from your eyes and 

towards the sample. 

• Colilert-18 results are definitive at 18–22 hours. In addition, positives for both 

total coliforms and E. coli observed before 18 hours and negatives observed 

after 22 hours are also valid.

Procedural Notes

• A slight tinge may be observed when Colilert-18 is added to the sample.

• If excess foam causes problems while using Quanti-Tray, you may choose to 

use IDEXX Antifoam Solution (Catalog# WAFDB) or IDEXX 120 ml vessels with 

Antifoam (Catalog# WV120SBAF-200).

• When following AFNOR regulations for drinking water testing, place the sealed 

Quanti-Tray in a 36°C ±2°C incubator for 18 hours (prewarming to 36°C is not 

required).

• This insert may not reflect your local regulations. For compliance testing, be 

sure to follow appropriate regulatory procedures.
• Colilert-18 can be run in any multiple tube format. Standard Methods for the 

Examination of Water and Wastewater MPN tables should be used to find Most 

Probable Numbers (MPNs).

• If a water sample has some background color, compare inoculated Colilert-18 

sample to a control blank of the same water sample.

• Colilert-18 can be used for E. coli (but not coliforms) in marine water. Samples 

must be diluted at least tenfold. Multiply the MPN value by the dilution factor to 

obtain the proper quantitative result.

• Use only sterile, nonbuffered, oxidant-free water for dilutions.

• Colilert-18 is a primary water test. Colilert-18 performance characteristics do 

not apply to samples altered by any pre-enrichment or concentration.

• In samples with excessive chlorine, a blue flash may be seen when adding 

Colilert-18. If this is seen, consider sample invalid and discontinue testing.

• Aseptic technique should always be followed when using Colilert-18. Dispose 

of in accordance with Good Laboratory Practices.

Quality Control Procedures

The following quality control procedure is recommended for each lot of Colilert-18:

1. Fill three sterile vessels with 100 mL sterile water and inoculate one with an 

E. coli, one with a total coliform and one with a non-coliform using one of the 

following products:

A. Quanti-CultTM3 E. coli, Klebsiella pneumoniae and Pseudomonas aeruginosa

B. EZ-QC®4 E. coli, Klebsiella oxytoca and Pseudomonas aeruginosa

C. A sterile loop of ATCC5 strains, E. coli ATCC 25922 or 11775, Klebsiella 

pneumoniae ATCC 31488 and Pseudomonas aeruginosa ATCC 10145 or 

27853

2. Follow the P/A Procedure or Quanti-Tray Enumeration Procedure above.

3. Results should match the Result Interpretation table above.

Note: For fecal coliforms, EZ-QC E. Coli and Pseudomonas aeruginosa should be 

used for quality control.

For IDEXX Technical Support, please call:

North/South America: 207-556-4630/1-800-321-0207

Europe: 00800 4339 9111

UK: +44 (0) 1638 676800

China: +86-21-61279528

Japan: +81-422-71-5921

Australia: 1 800 655 978
1 IDEXX P/A Comparator, catalog # WP104; Quanti-Tray Comparator #WQTC, or Quanti-Tray/2000 Comparator 

#WQT2KC

2 Eaton AD, Clesceri LS, Greenberg AE. Standard Methods for the Examination of Water and Wastewater. American 

Public Health Association, 1998, Washington, DC.

3 Quanti-CultTM cultures - IDEXX catalog # WKIT-1001

4 EZ-QC Cultures – IDEXX Catalog #WEZQ

5 American Type Culture Collection 1-800-638-6597

Colilert, Defined Substrate Technology, DST and Quanti-Tray are either trademarks or registered trademaks of IDEXX 

Laboratories, Inc. in the United States and/or other countries. Quanti-Cult is a trademark of Remel Inc.

© 2010 IDEXX Laboratories, Inc. All rights reserved.

Manufactured under one or more of the following U.S. patents: 4,925,789; 5,429,933; 5,518,892; 5,610,029; 5,620, 

865; 5,620,895; 5,753,456 and 5,780,259. Other U.S. and/or foreign patents issued or pending.

IDEXX Laboratories, Inc. 

One IDEXX Drive 

Westbrook, Maine 04092 USA

www.idexx.com/water

IDX 33/01 – 11/09

MÉTHODES ALTERNATIVES D’ANALYSE 

Certifiée par AFNOR Certification

www.afnor.fr

La méthode IDEXX Colilert®-18/Quanti-Tray® a reçu le 

droit d’usage de la Marque AFNOR Validation pour le 

dénombrement des E. coli et des coliformes dans l’eau 

de consommation humaine.
06-XXXXX-00

Colilert®

DRAFT

Introduction

Colilert either simultaneously detects total coliforms and E. coli or fecal coliforms in 

water. It is based on IDEXX’s patented Defined Substrate Technology® (DST®). When 

total coliforms metabolize Colilert’s nutrient-indicator, ONPG, the sample turns yellow. 

When E. coli metabolize Colilert’s nutrient-indicator, MUG, the sample fluoresces. 

Colilert can detect these bacteria at 1 cfu/100 mL within 24 hours even with as many 

as 2 million heterotrophic bacteria per 100 mL present.

Contents

•	 WP020I contains 20 Snap Packs for 100 mL samples 

•	 WP200I contains 200 Snap Packs for 100 mL samples

Storage

Store at 2°C–30°C away from light.

Presence/Absence (P/A) Procedure

1. Add contents of one pack to a 100 mL sample in a sterile, transparent, 

nonfluorescing vessel.

2. Cap vessel and shake.

3. Incubate at 35°C ± 0.5°C for 24 hours.

4. Read results according to Result Interpretation table below.

Quanti-Tray® Enumeration Procedure

1. Add contents of one pack to 

a 100 mL water sample in a 

sterile vessel.

2. Cap vessel and shake until 

dissolved.

3. Pour sample/reagent mixture into a 

Quanti-Tray or Quanti-Tray/2000 and 

seal in an IDEXX Quanti-Tray Sealer.

Colilert® Test Kit
4. Place the sealed tray in a 35°C ± 0.5°C (or 44.5°C ± 0.2°C for fecal coliforms) 

incubator for 24 hours.

5. Read results according to the Result Interpretation table below. Count the number 

of positive wells and refer to the MPN table provided with the trays to obtain a Most 

Probable Number.

Result Interpretation

 Appearance Result

Less yellow than 

the comparator*

Negative for total coliforms, 

E. coli and fecal coliforms

Yellow equal to or greater than 

the comparator* when incubated 

at 35°C ±0.5°C

Positive for total coliforms

Yellow and fluorescence equal to 

or greater than the comparator*

when incubated at 35°C ±0.5°C

Positive for E. coli

Yellow equal to or greater than 

the comparator* when incubated 

at 44.5°C ±0.2°C

Positive for fecal coliforms

• Look for fluorescence with a 6-watt, 365 nm UV light within 5 inches of the sample 

in a dark environment. Face light away from your eyes and towards the sample.

• Colilert results are definitive at 24–28 hours. In addition, positives for both total 

coliforms and E. coli observed before 24 hours and negatives observed after 28 

hours are also valid.

Procedural Notes

•	 This insert may not reflect your local regulations. For compliance testing, be sure

to follow appropriate regulatory procedures.

• 	 Colilert can be run in any multiple tube format. Standard Methods for the 

Examination of Water and Wastewater2 MPN tables should be used to find Most 

Probable Numbers (MPNs).

• 	 If a water sample has some background color, compare inoculated Colilert

sample to a control blank of the same water sample.

• 	 If sample dilutions are made, multiply the MPN value by the dilution factor to

obtain the proper quantitative result.
• 	 Use only sterile, nonbuffered, oxidant-free water for dilutions.

• 	 Colilert is a primary water test. Colilert performance characteristics do not apply to

samples altered by any pre-enrichment or concentration.

• 	 In samples with excessive chlorine, a blue flash may be seen when adding

Colilert. If this is seen, consider sample invalid and discontinue testing.

• 	 Aseptic technique should always be followed when using Colilert. Dispose of in

accordance with Good Laboratory Practices.

Quality Control Procedures

The following quality control procedure is recommended for each lot of Colilert:

1. Fill three sterile vessels with 100 mL sterile water and inoculate one with an 

E. coli, one with a total coliform and one with a non-coliform using one of the 

following products:

A. Quanti-CultTM3 E. coli, Klebsiella pneumoniae and Pseudomonas aeruginosa

B. EZ-QC®4 E. coli, Klebsiella oxytoca and Pseudomonas aeruginosa

C. A sterile loop of ATCC5 strains, E. coli ATCC 25922 or 11775, Klebsiella 

pneumoniae ATCC 31488 and Pseudomonas aeruginosa ATCC 10145 or 

27853

2. Follow the P/A Procedure or Quanti-Tray Enumeration Procedure above.

3. Results should match the Result Interpretation table above.

Note: For fecal coliforms, EZ-QC E. Coli and Pseudomonas aeruginosa should be used 

for quality control.

For IDEXX Technical Support, please call:

North/South America: 207-556-4630/1-800-321-0207

Europe: 00800 4339 9111

UK: +44 (0) 1638 676800

China: +86-21-61279528

Japan: +81-422-71-5921

Australia: 1 800 655 978
1

 IDEXX P/A Comparator, catalog #WP104; Quanti-Tray Comparator #WQTC, or Quanti-Tray/2000 Comparator #WQT2KC

2

 Eaton AD, Clesceri LS, Greenberg AE. Standard Methods for the Examination of Water and Wastewater. American Public 

Health Association, 1998, Washington, DC.

3

 Quanti-Cult cultures – IDEXX catalog # WKIT-1001

4 EZ-QC Cultures – IDEXX Catalog #WEZQ

5

 American Type Culture Collection 1-800-638-6597

Colilert, Defined Substrate Technology, DST and Quanti-tray are either trademarks or registered trademarks of IDEXX 

Laboratories, Inc. in the United States and/or other countries. Quanti-Cult is a trademark of Remel Inc.

© 2010 IDEXX Laboratories, Inc. All rights reserved.

Manufactured under one or more of the following U.S. patents: 4,925,789; 5,429,933; 5,518,892; 5,610,029; 5,620, 865; 

5,620,895; 5,753,456 and 5,780,259. Other U.S. and/or foreign patents issued or pending.

IDEXX Laboratories, Inc. 

One IDEXX Drive 

Westbrook, Maine 04092 USA

www.idexx.com/water
IDEXX Quanti-Tray

Insert and Most Probable Number (MPN) Table

06-02030-12

IDEXX Laboratories, Inc.

One IDEXX Drive

Westbrook, Maine 04092 USA

www.idexx.com/water

This certifies that the enclosed Quanti-Trays have 

been sterilized with ethylene oxide.

For further information or documentation, contact 

IDEXX Laboratories, Inc.

North/South America: 1-207-556-4496/1-800-321-0207

Europe: 00800 4339 9111

UK: +44 (0) 1638 676800

China: +86-21-61279528

Japan: +81 422 71 5921

Australia: 1800 655 978

Quanti-Tray Certificate of Sterility

IDEXX Quanti-Tray

English Version

Quanti-Tray and Defined Substrate Technology are trademarks or registered trademarks of IDEXX Laboratories, Inc. in the 

United States and/or other countries. Covered by U.S. Patent Nos. 5,518,892; 5,620,895; and/or 5,753,456. Other patents pending.

Introduction

IDEXX Quanti-Trays are designed to give quantitated bacterial counts of 100 mL samples using IDEXX Defined Substrate Technology 

reagent products. Add the reagent/sample mixture to a Quanti-Tray, seal it in a Quanti-Tray Sealer and incubate per the reagent 

instructions. Then count the number of positive wells and use the Most Probable Number (MPN) Table attached to determine the MPN.

Contents

This package contains sterile 51-well Quanti-Trays.

For Technical Assistance

U.S./Canada 1-800-321-0207 or 1-207-556-4496

www.idexx.com/water

User Instructions

1. Use one hand to hold a Quanti-Tray 

upright with the well side facing 

the palm.

2. Squeeze the upper part of the QuantiTray so that the Quanti-Tray bends 

toward the palm.

3. Open the Quanti-Tray by pulling the 

foil tab away from the well side. Avoid 

touching the inside of the foil or tray.

4. Pour the reagent/sample mixture 

directly into the Quanti-Tray, avoiding 

contact with the foil tab. Allow foam 

to settle.

5. Place the sample-filled Quanti-Tray 

onto the rubber tray carrier of the 

Quanti-Tray Sealer with the well side 

(plastic) of the Quanti-Tray facing 

down to fit into the carrier.

6. Seal according to the Quanti-Tray 

Sealer instructions.

7. Incubate according to reagent 

instructions.

8. Count positive wells and refer to 

the MPN table on the back of this 

instruction sheet to find the MPN.

9. Dispose of media in accordance with 

good laboratory practices.

IDEXX Laboratories, Inc.

One IDEXX Drive

Westbrook, Maine 04092 USA
51-Well Quanti-Tray MPN Table

No. of wells giving 

positive reaction per 

100 mL sample

Most Probable 

Number (MPN)

95% Confidence Limits

Lower Upper

0 <1.0 0.0 3.7

1 1.0 0.3 5.6

2 2.0 0.6 7.3

3 3.1 1.1 9.0

4 4.2 1.7 10.7

5 5.3 2.3 12.3

6 6.4 3.0 13.9

7 7.5 3.7 15.5

8 8.7 4.5 17.1

9 9.9 5.3 18.8

10 11.1 6.1 20.5

11 12.4 7.0 22.1

12 13.7 7.9 23.9

13 15.0 8.8 25.7

14 16.4 9.8 27.5

15 17.8 10.8 29.4

16 19.2 11.9 31.3

17 20.7 13.0 33.3

18 22.2 14.1 35.2

19 23.8 15.3 37.3

20 25.4 16.5 39.4

21 27.1 17.7 41.6

22 28.8 19.0 43.9

23 30.6 20.4 46.3

24 32.4 21.8 48.7

25 34.4 23.3 51.2

26 36.4 24.7 53.9

27 38.4 26.4 56.6

28 40.6 28.0 59.5

29 42.9 29.7 62.5

30 45.3 31.5 65.6

31 47.8 33.4 69.0

32 50.4 35.4 72.5

33 53.1 37.5 76.2

34 56.0 39.7 80.1

35 59.1 42.0 84.4

36 62.4 44.6 88.8

37 65.9 47.2 93.7

38 69.7 50.0 99.0

39 73.8 53.1 104.8

40 78.2 56.4 111.2

41 83.1 59.9 118.3

42 88.5 63.9 126.2

43 94.5 68.2 135.4

44 101.3 73.1 146.0

45 109.1 78.6 158.7

46 118.4 85.0 174.5

47 129.8 92.7 195.0

48 144.5 102.3 224.1

49 165.2 115.2 272.2

50 200.5 135.8 387.6

51 > 200.5 146.1 infinite
IDEXX Quanti-Tray

Version Française

Quanti-Tray et Defined Substrate Technology sont des marques de fabrique ou des marques déposées 

d'IDEXX Laboratories, Inc. aux États-Unis et/ou dans d'autres pays. Protégé par les brevets américains 

n° 5,518,892; 5,620,895; and/or 5,753,456. Autres brevets en cours d'enregistrement.

Introduction

Les plateaux Quanti-Tray d’IDEXX, spécialement conçus pour des numérations bactériennes sur des échantillons de 100 millilitres, font 

appel à la technologie Defined Substrate Technology d’IDEXX basée sur l’utilisation de réactifs. Versez le mélange réactif/échantillon 

dans un Quanti-Tray, placez ensuite le tout dans le Quanti-Tray Sealer et procédez à l’incubation du mélange selon les instructions 

accompagnant les réactifs. Comptez ensuite le nombre de cupules positives et référez-vous à la table ci-dessous pour déterminer le 

nombre le plus probable (NPP).

Contentu

Cette boîte contient 100 Quanti-Tray stériles composés chacun de 51 cupules.

Services techniques IDEXX

00800-4339-9111

www.idexx.fr/eau

Mode d'emploi

1. Prenez un Quanti-Tray et tenez-le à 

la verticale en veillant à orienter les 

cupules vers la paume de la main.

2. Appuyez sur la partie supérieure du 

Quanti-Tray afin qu’il vienne se loger 

dans la paume de la main.

3. Ouvrez le Quanti-Tray en écartant la 

languette en aluminium des cupules. 

Evitez tout contact avec la partie 

intérieure de l’aluminium ou du plateau.

4. Versez directement le mélange 

réactif/échantillon dans le QuantiTray en évitant tout contact avec la 

languette d’aluminium. Laisser la 

mousse se dissiper.

5. Placez le Quanti-Tray rempli 

d’échantillons sur le porte-plateau en 

caoutchouc du Quanti-Tray Sealer en 

veillant à orienter les cupules (parties 

en plastique) vers le bas afin qu’elles 

viennent s’encastrer parfaitement 

dans le porte-plateau.

6. Scellez le Quanti-Tray conformément 

aux instructions accompagnant le 

Sealer.

7. Incubez conformément aux 

instructions accompagnant le réactif.

8. Comptez le nombre de cupules 

positives, puis référez-vous à la table 

NPP au verso pour déterminer le 

nombre le plus probable (NPP).

9. Débarrassez-vous du milieu en suivant 

les bonnes pratiques de laboratoire.

IDEXX Laboratories, Inc.

One IDEXX Drive

Westbrook, Maine 04092 USA
Table NPP du Quanti-Tray a “51 Cupules”

Nombre de cupules 

donnant une 

réaction positive par 

échantillon de 100 ml 

Nombre le plus 

probable

Limites de confiance à 95%

Minimum Maximum

0 <1.0 0.0 3.7

1 1.0 0.3 5.6

2 2.0 0.6 7.3

3 3.1 1.1 9.0

4 4.2 1.7 10.7

5 5.3 2.3 12.3

6 6.4 3.0 13.9

7 7.5 3.7 15.5

8 8.7 4.5 17.1

9 9.9 5.3 18.8

10 11.1 6.1 20.5

11 12.4 7.0 22.1

12 13.7 7.9 23.9

13 15.0 8.8 25.7

14 16.4 9.8 27.5

15 17.8 10.8 29.4

16 19.2 11.9 31.3

17 20.7 13.0 33.3

18 22.2 14.1 35.2

19 23.8 15.3 37.3

20 25.4 16.5 39.4

21 27.1 17.7 41.6

22 28.8 19.0 43.9

23 30.6 20.4 46.3

24 32.4 21.8 48.7

25 34.4 23.3 51.2

26 36.4 24.7 53.9

27 38.4 26.4 56.6

28 40.6 28.0 59.5

29 42.9 29.7 62.5

30 45.3 31.5 65.6

31 47.8 33.4 69.0

32 50.4 35.4 72.5

33 53.1 37.5 76.2

34 56.0 39.7 80.1

35 59.1 42.0 84.4

36 62.4 44.6 88.8

37 65.9 47.2 93.7

38 69.7 50.0 99.0

39 73.8 53.1 104.8

40 78.2 56.4 111.2

41 83.1 59.9 118.3

42 88.5 63.9 126.2

43 94.5 68.2 135.4

44 101.3 73.1 146.0

45 109.1 78.6 158.7

46 118.4 85.0 174.5

47 129.8 92.7 195.0

48 144.5 102.3 224.1

49 165.2 115.2 272.2

50 200.5 135.8 387.6

51 > 200.5 146.1 infini
IDEXX Quanti-Tray

Version Italiano

Quanti-Tray e Defined Substrate Technology sono marchi di proprietà di, e/o registrati da, IDEXX Laboratories, Inc. 

e protetti negli Stati Uniti e/o in altri paesi. Protetto dai brevetti degli U.S.A. nn. 5,518,892; 5,620,895; and/or 5,753,456. 

Altre domande di brevetto pendenti.

Introduzione

Quanti-Tray IDEXX sono concepiti per fornire la densità batterica (quantitativa) di campioni da 100 mL utilizzando reagenti Defined 

Substrate Technology IDEXX. Aggiungere la miscela di reagente/campione ad un Quanti-Tray, inserire quest’ultimo nel Quanti-Tray Sealer 

ed incubare in conformità alle istruzioni fornite con il reagente. Contare quindi il numero di pozzetti positivi e, con l’ausilio della tabella 

MPN allegata, determinare il numero più probabile (MPN).

Contenuto

Questa confezione contiene Quanti-Tray sterili, a 51 pozzetti.

Supporto Tecnico IDEXX

00800-4339-9111

www.idexx.it/acqua

Istruzioni per l’uso

1. Con una mano, tenere il Quanti-Tray 

in posizione verticale, con il lato dei 

pozzetti rivolto verso il palmo.

2. Comprimere la parte superiore del 

Quanti-Tray in modo da fletterlo verso 

il palmo.

3. Aprire il Quanti-Tray tirando la linguetta 

in alluminio in direzione opposta al lato 

dei pozzetti. Evitare di toccare la parte 

interna del foglio protettivo in alluminio 

o del dispositivo a pozzetti.

4. Versare direttamente la miscela di 

reagente/campione nel Quanti-Tray, 

evitando di toccare la linguetta in 

alluminio. Consentire alla schiuma 

di sedimentare.

5. Una volta versata la miscela, collocare 

il Quanti-Tray nel portapozzetti in 

gomma del Quanti-Tray Sealer, con 

il lto dei pozzetti (in plastica) rivolto 

verso il basso, in modo da assicurarne 

il corretto posizionamento.

6. Sigillare secondo le istruzioni del 

Quanti-Tray Sealer.

7. Incubare secondo le istruzioni fornite 

con il reagente.

8. Contare i pozzetti positivi e consultare 

la tabella MPN sul retro del foglio delle 

istruzioni, per individuare il numero più 

probabile. (MPN) corretto.

9. Eliminare el terreno in accordo alle 

Buone pratiche di laboratorio.

IDEXX Laboratories, Inc.

One IDEXX Drive

Westbrook, Maine 04092 USA
Tabella MPN Quanti-Tray a 51 pozzetti

N° di pozzetti positivi 

in un campione da 

100 ml

Numero piú 

probabile

Limiti fiduciali del 

95% Inferiore

Limiti fiduciali del 

95% Superiore

0 <1.0 0.0 3.7

1 1.0 0.3 5.6

2 2.0 0.6 7.3

3 3.1 1.1 9.0

4 4.2 1.7 10.7

5 5.3 2.3 12.3

6 6.4 3.0 13.9

7 7.5 3.7 15.5

8 8.7 4.5 17.1

9 9.9 5.3 18.8

10 11.1 6.1 20.5

11 12.4 7.0 22.1

12 13.7 7.9 23.9

13 15.0 8.8 25.7

14 16.4 9.8 27.5

15 17.8 10.8 29.4

16 19.2 11.9 31.3

17 20.7 13.0 33.3

18 22.2 14.1 35.2

19 23.8 15.3 37.3

20 25.4 16.5 39.4

21 27.1 17.7 41.6

22 28.8 19.0 43.9

23 30.6 20.4 46.3

24 32.4 21.8 48.7

25 34.4 23.3 51.2

26 36.4 24.7 53.9

27 38.4 26.4 56.6

28 40.6 28.0 59.5

29 42.9 29.7 62.5

30 45.3 31.5 65.6

31 47.8 33.4 69.0

32 50.4 35.4 72.5

33 53.1 37.5 76.2

34 56.0 39.7 80.1

35 59.1 42.0 84.4

36 62.4 44.6 88.8

37 65.9 47.2 93.7

38 69.7 50.0 99.0

39 73.8 53.1 104.8

40 78.2 56.4 111.2

41 83.1 59.9 118.3

42 88.5 63.9 126.2

43 94.5 68.2 135.4

44 101.3 73.1 146.0

45 109.1 78.6 158.7

46 118.4 85.0 174.5

47 129.8 92.7 195.0

48 144.5 102.3 224.1

49 165.2 115.2 272.2

50 200.5 135.8 387.6

51 > 200.5 146.1 Infinito
IDEXX Quanti-Tray

Versíon Español

Quanti-Tray y Defined Substrate Technology son marcas o marcas registradas de IDEXX Laboratories, Inc. en los 

Estados Unidos y/o otros paises. Patente norteamericanas numero 5,518,892; 5,620,895; and/or 5,753,456. Otras patentes 

pendientes de concesión.

Introducción

Los dispositivos Quanti-Tray de IDEXX están diseñados para producir recuentos bacterianos cuantificados de muestras de 100 mL, al 

ser utilizadas con productos de reactivos de la Tecnología del Sustrato Definido (Defined Substrate Technology) de IDEXX. Agregue la 

mezcla de reactivo y muestra a un dispositivo Quanti-Tray, séllelo en el Quanti-Tray Sealer (Sellador) e incúbela según las instrucciones del 

reactivo. Luego cuente el número de celdas positivas y utilice la tabla de NMP adjunta para determinar el Número Más Probable (NMP).

Contenido

Este paquete contiene 100 dispositivos Quanti-Tray estériles, de 51 celdas c/u.

Soporte técnico de IDEXX

00800-4339-9111

www.idexx.es/agua

Instrucciones para el usuario

1. Sostenga en una mano el dispositivo 

Quanti-Tray en posición vertical, con 

el lado de las celdas orientado hacia 

la palma.

2. Apriete la parte superior de el 

dispositivo Quanti-Tray de modo 

de doblarla hacia la palma.

3. Abra el dispositivo Quanti-Tray 

desprendiendo la lengüeta metálica del 

lado que contiene las celdas. Evite tocar 

el interior del metal o del dispositivo.

4. Vierta la mezcla de reactivo y la 

muestra directamente dentro de el 

dispositivo Quanti-Tray, evitando tocar 

la lengüeta metálica. Deja reposar 

la espuma.

5. Coloque el dispositivo Quanti-Tray

0lleno de muestra sobre el 

portadispositivo de goma del sellador 

Quanti-Tray, orientando el lado de las 

celdas (plásticas) del dispositivo hacia 

abajo de manera que quepa en el 

portadispositivo.

6. Selle el dispositivo según las 

instrucciones del sellador.

7. Incube de acuerdo con las 

instrucciones del reactivo.

8. Cuente las celdas positivas. Para 

determinar el número más probable, 

recurra a la tabla NMP al dorso de 

esta hoja de instrucciones.

9. Verter el medio de cultivo conforme a 

Buenas Prácticas del Laboratorio.

IDEXX Laboratories, Inc.

One IDEXX Drive

Westbrook, Maine 04092 USA
Tabla de NMP para dispositivo Quanti-Tray de 51 celdas

No de celdas que 

producen una 

reacción positiva, por 

muestra de 100 ml

Número más 

probable

Límites de confianza del 95%

Inferior Superior

0 <1.0 0.0 3.7

1 1.0 0.3 5.6

2 2.0 0.6 7.3

3 3.1 1.1 9.0

4 4.2 1.7 10.7

5 5.3 2.3 12.3

6 6.4 3.0 13.9

7 7.5 3.7 15.5

8 8.7 4.5 17.1

9 9.9 5.3 18.8

10 11.1 6.1 20.5

11 12.4 7.0 22.1

12 13.7 7.9 23.9

13 15.0 8.8 25.7

14 16.4 9.8 27.5

15 17.8 10.8 29.4

16 19.2 11.9 31.3

17 20.7 13.0 33.3

18 22.2 14.1 35.2

19 23.8 15.3 37.3

20 25.4 16.5 39.4

21 27.1 17.7 41.6

22 28.8 19.0 43.9

23 30.6 20.4 46.3

24 32.4 21.8 48.7

25 34.4 23.3 51.2

26 36.4 24.7 53.9

27 38.4 26.4 56.6

28 40.6 28.0 59.5

29 42.9 29.7 62.5

30 45.3 31.5 65.6

31 47.8 33.4 69.0

32 50.4 35.4 72.5

33 53.1 37.5 76.2

34 56.0 39.7 80.1

35 59.1 42.0 84.4

36 62.4 44.6 88.8

37 65.9 47.2 93.7

38 69.7 50.0 99.0

39 73.8 53.1 104.8

40 78.2 56.4 111.2

41 83.1 59.9 118.3

42 88.5 63.9 126.2

43 94.5 68.2 135.4

44 101.3 73.1 146.0

45 109.1 78.6 158.7

46 118.4 85.0 174.5

47 129.8 92.7 195.0

48 144.5 102.3 224.1

49 165.2 115.2 272.2

50 200.5 135.8 387.6

51 > 200.5 146.1 infinito
IDEXX Quanti-Tray

Deutsch Version

Quanti-Tray und Defined Substrate Technology sind Schutzmarken oder eingetragene Schutzmarken von 

IDEXX Laboratories, Inc. in den Vereinigten Staaten und/oder in anderen Ländern. Geschützt durch U.S.-Patent 

Nummern 5,518,892; 5,620,895; and/or 5,753,456. Andere Patenten angemeldet.

Einführung

Quanti-Tray von IDEXX dienen zur quantitativen Bakterienzählung in 100-mL-Proben unter Verwendung von IDEXX Defined 

Substrate Technology-Reagenzien. Die Mischung aus Probe und Reagenz in einen Quanti-Tray geben, diesen in einem Quanti-TrayVersiegelungsgerät verschließen und laut Reagenz-Packungsbeilage inkubieren. Anschließend die Anzahl der positiven Vertiefungen 

zählen und mit der beigefügten MPN-Tabelle die “Wahrscheinlichste Anzahl” (MPN) bestimmen.

Inhalt

Diese Packung enthält 100 sterile Quanti-Tray mit jeweils 51 Vertiefungen.

IDEXX Technischer Dienst

00800-4339-9111

www.idexx.de/wasser

Gebrauchsanweisung

1. Quanti-Tray mit einer Hand fassen und 

senkrecht halten, wobei die Seite mit 

den Vertiefungen zur Handfläche zeigt.

2. Den oberen Teil des Quanti-Trays 

zusammendrücken, so daß sich der 

Träger zur Handfläche hin biegt.

3. Quanti-Tray öffnen, indem die 

Folienlasche von der Seite mit den 

Vertiefungen abgezogen wird. Die 

Innenseite der Folie oder des Trays 

nicht berühren.

4. Die Mischung aus Probe und 

Reagenz direkt in einen Quanti-Tray 

gießen. Dabei eine Berührung der 

Folienlasche vermeiden. Schaum 

einwirken lassen.

5. Den mit der Probe gefüllten QuantiTray auf die Gummi-Trägerunterlage 

des Quanti-Tray-Versiegelungsgeräts 

stellen. Die Seite mit den Vertiefungen 

(Kunststoff) des Quanti-Trays muß 

dabei nach unten weisen, damit sie in 

die Unterlage paßt.

6. Das Verschließen erfolgt 

laut Anweisungen für das 

Versiegelungsgerät.

7. Entsprechend den 

Reagenzanweisungen inkubieren.

8. Die positiven Vertiefungen zählen 

und mit Hilfe der MPN-Tabelle auf der 

Rückseite dieser Packungsbeilage 

die Wahrscheinlichste Anzahl (MPN) 

ermitteln.

9. Entsorgen Sie die Quanti-Trays gemäß 

guter Laborpraxis.

IDEXX Laboratories, Inc.

One IDEXX Drive

Westbrook, Maine 04092 USA
MPN-Tabelle für Quanti-Tray mit 51 Vertiefungen

Anzahl der 

Vertiefungen, die bei 

einer 100-ml-Probe

positiv reagieren

Wahrscheinlichste 

Anzahl

95% Vertrauensgrenze

Untere Obere

0 <1.0 0.0 3.7

1 1.0 0.3 5.6

2 2.0 0.6 7.3

3 3.1 1.1 9.0

4 4.2 1.7 10.7

5 5.3 2.3 12.3

6 6.4 3.0 13.9

7 7.5 3.7 15.5

8 8.7 4.5 17.1

9 9.9 5.3 18.8

10 11.1 6.1 20.5

11 12.4 7.0 22.1

12 13.7 7.9 23.9

13 15.0 8.8 25.7

14 16.4 9.8 27.5

15 17.8 10.8 29.4

16 19.2 11.9 31.3

17 20.7 13.0 33.3

18 22.2 14.1 35.2

19 23.8 15.3 37.3

20 25.4 16.5 39.4

21 27.1 17.7 41.6

22 28.8 19.0 43.9

23 30.6 20.4 46.3

24 32.4 21.8 48.7

25 34.4 23.3 51.2

26 36.4 24.7 53.9

27 38.4 26.4 56.6

28 40.6 28.0 59.5

29 42.9 29.7 62.5

30 45.3 31.5 65.6

31 47.8 33.4 69.0

32 50.4 35.4 72.5

33 53.1 37.5 76.2

34 56.0 39.7 80.1

35 59.1 42.0 84.4

36 62.4 44.6 88.8

37 65.9 47.2 93.7

38 69.7 50.0 99.0

39 73.8 53.1 104.8

40 78.2 56.4 111.2

41 83.1 59.9 118.3

42 88.5 63.9 126.2

43 94.5 68.2 135.4

44 101.3 73.1 146.0

45 109.1 78.6 158.7

46 118.4 85.0 174.5

47 129.8 92.7 195.0

48 144.5 102.3 224.1

49 165.2 115.2 272.2

50 200.5 135.8 387.6

51 > 200.5 146.1 endlos
IDEXX Quanti-Tray

日本語版

Quanti-Tray および Defined Substrate Technology は米国および / または他国の IDEXX Laboratories, Inc. の商標または登録商標です。 以下の特許番

号の米国特許により保護されています。 5,518,892; 5,620,895; and/or 5,753,456. 他の特許は出願中です。

はじめに

IDEXX Quanti-Tray(QT-トレイ)は、IDEXXが特許を取得しているDefined Substrate Technology( 特定酵素基質法)に基づいたColilertを用い、検体

100mL中の最確数を測定する新しいシステムです。Colilertと検体の混合液をQuanti-Trayに加え、シーラーで封入し、取扱説明書に従い培養して

ください。その後、陽性ウェル数を数え、添付の最確数(MPN)表を用いて、MPNを測定します。

内容

このパッケージには、滅菌済みの 51 ウェル Quanti-Tray が 100個入っています。

ご質問やサポートに関しては以下にご連絡ください。

アイデックス ラボラトリーズ株式会社

TEL: 0422-71-5921 www.idexx.co.jp/water

操作手順 

1. ウェル側を手のひらに向けてトレイを垂直

に持ってください。

2. トレイが手のひらに向かって曲がるよう

に、トレイの上部を強く握ってください。

3. トレイのつまみをウェル側から引き離し

て、注入口を広げてください。トレイのつ

まみ、またはトレイの内部に触れないよう

にしてください。

4. トレイのつまみにかからないように直接、

Colilertと検体の混合液を注いでくださ

い。気泡は軽く指ではじくように取り除い

てください。

5. 検体の入ったトレイをシーラーのゴム製シ

ートの上にウェル側を下に向けてセットし

てください。

6. シーラーの取扱説明書に従って密封して

ください。

7. Colilertの取扱説明書に従って培養してく

ださい。

8. 陽性ウェル数を数え、添付の最確数

(MPN) 表を使用してMPNを求めてくだ

さい。

9. 検体及びトレイの廃棄は、高圧滅菌してく

ださい。

IDEXX Laboratories, Inc.

One IDEXX Drive

Westbrook, Maine 04092 USA
51 ウェル Quanti-Tray MPN 表

100ml の検体で陽性

反応を呈するウェル数 最確数 (MPN)

95% 信頼限界

下限 上限

0 <1.0 0.0 3.7

1 1.0 0.3 5.6

2 2.0 0.6 7.3

3 3.1 1.1 9.0

4 4.2 1.7 10.7

5 5.3 2.3 12.3

6 6.4 3.0 13.9

7 7.5 3.7 15.5

8 8.7 4.5 17.1

9 9.9 5.3 18.8

10 11.1 6.1 20.5

11 12.4 7.0 22.1

12 13.7 7.9 23.9

13 15.0 8.8 25.7

14 16.4 9.8 27.5

15 17.8 10.8 29.4

16 19.2 11.9 31.3

17 20.7 13.0 33.3

18 22.2 14.1 35.2

19 23.8 15.3 37.3

20 25.4 16.5 39.4

21 27.1 17.7 41.6

22 28.8 19.0 43.9

23 30.6 20.4 46.3

24 32.4 21.8 48.7

25 34.4 23.3 51.2

26 36.4 24.7 53.9

27 38.4 26.4 56.6

28 40.6 28.0 59.5

29 42.9 29.7 62.5

30 45.3 31.5 65.6

31 47.8 33.4 69.0

32 50.4 35.4 72.5

33 53.1 37.5 76.2

34 56.0 39.7 80.1

35 59.1 42.0 84.4

36 62.4 44.6 88.8

37 65.9 47.2 93.7

38 69.7 50.0 99.0

39 73.8 53.1 104.8

40 78.2 56.4 111.2

41 83.1 59.9 118.3

42 88.5 63.9 126.2

43 94.5 68.2 135.4

44 101.3 73.1 146.0

45 109.1 78.6 158.7

46 118.4 85.0 174.5

47 129.8 92.7 195.0

48 144.5 102.3 224.1

49 165.2 115.2 272.2

50 200.5 135.8 387.6

51 > 200.5 146.1 以上
IDEXX Quanti-Tray/2000

Insert and Most Probable Number (MPN) Table

06-02320-10

IDEXX Laboratories, Inc.

One IDEXX Drive

Westbrook, Maine 04092 USA

This certifies that the enclosed Quanti-Trays have 

been sterilized with ethylene oxide.

For further information or documentation, contact 

IDEXX Laboratories, Inc.

North/South America: 1-207-556-4496/1-800-321-0207

Europe: 00800 4339 9111

UK: +44 (0) 1638 676800

China: +86-21-61279528

Japan: +81 422 71 5921

Australia: 1800 655 978

Quanti-Tray Certificate of Sterility
IDEXX Quanti-Tray/2000

English Version

Quanti-Tray and Defined Substrate Technology are trademarks or registered trademarks of IDEXX Laboratories, Inc. in the 

United States and/or other countries. Covered by U.S. Patent Numbers 5,518,892; 5,620,895; and 5,753,456. Other patents pending.

Introduction

IDEXX Quanti-Tray/2000 is designed to give quantitated bacterial counts of 100 mL samples using IDEXX Defined Substrate Technology 

reagent products. Add the reagent/sample mixture to a Quanti-Tray/2000, seal it in a Quanti-Tray Sealer and incubate per the reagent 

instructions. Then count the number of positive large and small wells and use the Most Probable Number (MPN) Table attached to 

determine the MPN.

Contents

This package contains sterile Quanti-Tray/2000s.

For Technical Support

U.S./Canada 1-800-321-0207 or 1-207-556-4496

www.idexx.com/water

User Instructions

1. Use one hand to hold a Quanti-Tray 

upright with the well side facing 

the palm.

2. Squeeze the upper part of the QuantiTray so that the Quanti-Tray bends 

toward the palm.

3. Gently pull foil tab to separate the 

foil from the tray. Avoid touching the 

inside of the foil or tray.

4. Pour the reagent/sample mixture 

directly into the Quanti-Tray, avoiding 

contact with the foil tab. Tap the small 

wells 2–3 times to release any air 

bubbles. Allow foam to settle.

5. Place the sample-filled Quanti-Tray 

onto the Quanti-Tray/2000 rubber 

insert of the Quanti-Tray Sealer with 

the well side (plastic) of the QuantiTray facing down.

6. Seal according to the Quanti-Tray 

Sealer instructions.

7. Incubate according to reagent 

instructions.

8. Count large and small positive wells 

and refer to the Quanti-Tray/2000 MPN 

table to find the MPN.

9. Dispose of media in accordance with 

good laboratory practices.

IDEXX Laboratories, Inc.

One IDEXX Drive

Westbrook, Maine 04092 USA
IDEXX Quanti-Tray/2000 MPN Table

# Large 

Wells 

Positive

# Small Wells Positive

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

0 <1 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.1 15.1 16.1 17.1 18.1 19.1 20.2 21.2 22.2 23.3 24.31 1.0 2.0 3.0 4.0 5.0 6.0 7.1 8.1 9.1 10.1 11.1 12.1 13.2 14.2 15.2 16.2 17.3 18.3 19.3 20.4 21.4 22.4 23.5 24.5 25.62 2.0 3.0 4.1 5.1 6.1 7.1 8.1 9.2 10.2 11.2 12.2 13.3 14.3 15.4 16.4 17.4 18.5 19.5 20.6 21.6 22.7 23.7 24.8 25.8 26.93 3.1 4.1 5.1 6.1 7.2 8.2 9.2 10.3 11.3 12.4 13.4 14.5 15.5 16.5 17.6 18.6 19.7 20.8 21.8 22.9 23.9 25.0 26.1 27.1 28.24 4.1 5.2 6.2 7.2 8.3 9.3 10.4 11.4 12.5 13.5 14.6 15.6 16.7 17.8 18.8 19.9 21.0 22.0 23.1 24.2 25.3 26.3 27.4 28.5 29.65 5.2 6.3 7.3 8.4 9.4 10.5 11.5 12.6 13.7 14.7 15.8 16.9 17.9 19.0 20.1 21.2 22.2 23.3 24.4 25.5 26.6 27.7 28.8 29.9 31.06 6.3 7.4 8.4 9.5 10.6 11.6 12.7 13.8 14.9 16.0 17.0 18.1 19.2 20.3 21.4 22.5 23.6 24.7 25.8 26.9 28.0 29.1 30.2 31.3 32.47 7.5 8.5 9.6 10.7 11.8 12.8 13.9 15.0 16.1 17.2 18.3 19.4 20.5 21.6 22.7 23.8 24.9 26.0 27.1 28.3 29.4 30.5 31.6 32.8 33.98 8.6 9.7 10.8 11.9 13.0 14.1 15.2 16.3 17.4 18.5 19.6 20.7 21.8 22.9 24.1 25.2 26.3 27.4 28.6 29.7 30.8 32.0 33.1 34.3 35.49 9.8 10.9 12.0 13.1 14.2 15.3 16.4 17.6 18.7 19.8 20.9 22.0 23.2 24.3 25.4 26.6 27.7 28.9 30.0 31.2 32.3 33.5 34.6 35.8 37.0

10 11.0 12.1 13.2 14.4 15.5 16.6 17.7 18.9 20.0 21.1 22.3 23.4 24.6 25.7 26.9 28.0 29.2 30.3 31.5 32.7 33.8 35.0 36.2 37.4 38.6

11 12.2 13.4 14.5 15.6 16.8 17.9 19.1 20.2 21.4 22.5 23.7 24.8 26.0 27.2 28.3 29.5 30.7 31.9 33.0 34.2 35.4 36.6 37.8 39.0 40.2

12 13.5 14.6 15.8 16.9 18.1 19.3 20.4 21.6 22.8 23.9 25.1 26.3 27.5 28.6 29.8 31.0 32.2 33.4 34.6 35.8 37.0 38.2 39.5 40.7 41.913 14.8 16.0 17.1 18.3 19.5 20.6 21.8 23.0 24.2 25.4 26.6 27.8 29.0 30.2 31.4 32.6 33.8 35.0 36.2 37.5 38.7 39.9 41.2 42.4 43.614 16.1 17.3 18.5 19.7 20.9 22.1 23.3 24.5 25.7 26.9 28.1 29.3 30.5 31.7 33.0 34.2 35.4 36.7 37.9 39.1 40.4 41.6 42.9 44.2 45.415 17.5 18.7 19.9 21.1 22.3 23.5 24.7 25.9 27.2 28.4 29.6 30.9 32.1 33.3 34.6 35.8 37.1 38.4 39.6 40.9 42.2 43.4 44.7 46.0 47.316 18.9 20.1 21.3 22.6 23.8 25.0 26.2 27.5 28.7 30.0 31.2 32.5 33.7 35.0 36.3 37.5 38.8 40.1 41.4 42.7 44.0 45.3 46.6 47.9 49.217 20.3 21.6 22.8 24.1 25.3 26.6 27.8 29.1 30.3 31.6 32.9 34.1 35.4 36.7 38.0 39.3 40.6 41.9 43.2 44.5 45.9 47.2 48.5 49.8 51.218 21.8 23.1 24.3 25.6 26.9 28.1 29.4 30.7 32.0 33.3 34.6 35.9 37.2 38.5 39.8 41.1 42.4 43.8 45.1 46.5 47.8 49.2 50.5 51.9 53.219 23.3 24.6 25.9 27.2 28.5 29.8 31.1 32.4 33.7 35.0 36.3 37.6 39.0 40.3 41.6 43.0 44.3 45.7 47.1 48.4 49.8 51.2 52.6 54.0 55.420 24.9 26.2 27.5 28.8 30.1 31.5 32.8 34.1 35.4 36.8 38.1 39.5 40.8 42.2 43.6 44.9 46.3 47.7 49.1 50.5 51.9 53.3 54.7 56.1 57.621 26.5 27.9 29.2 30.5 31.8 33.2 34.5 35.9 37.3 38.6 40.0 41.4 42.8 44.1 45.5 46.9 48.4 49.8 51.2 52.6 54.1 55.5 56.9 58.4 59.922 28.2 29.5 30.9 32.3 33.6 35.0 36.4 37.7 39.1 40.5 41.9 43.3 44.8 46.2 47.6 49.0 50.5 51.9 53.4 54.8 56.3 57.8 59.3 60.8 62.323 29.9 31.3 32.7 34.1 35.5 36.8 38.3 39.7 41.1 42.5 43.9 45.4 46.8 48.3 49.7 51.2 52.7 54.2 55.6 57.1 58.6 60.2 61.7 63.2 64.724 31.7 33.1 34.5 35.9 37.3 38.8 40.2 41.7 43.1 44.6 46.0 47.5 49.0 50.5 52.0 53.5 55.0 56.5 58.0 59.5 61.1 62.6 64.2 65.8 67.325 33.6 35.0 36.4 37.9 39.3 40.8 42.2 43.7 45.2 46.7 48.2 49.7 51.2 52.7 54.3 55.8 57.3 58.9 60.5 62.0 63.6 65.2 66.8 68.4 70.026 35.5 36.9 38.4 39.9 41.4 42.8 44.3 45.9 47.4 48.9 50.4 52.0 53.5 55.1 56.7 58.2 59.8 61.4 63.0 64.7 66.3 67.9 69.6 71.2 72.927 37.4 38.9 40.4 42.0 43.5 45.0 46.5 48.1 49.6 51.2 52.8 54.4 56.0 57.6 59.2 60.8 62.4 64.1 65.7 67.4 69.1 70.8 72.5 74.2 75.928 39.5 41.0 42.6 44.1 45.7 47.3 48.8 50.4 52.0 53.6 55.2 56.9 58.5 60.2 61.8 63.5 65.2 66.9 68.6 70.3 72.0 73.7 75.5 77.3 79.029 41.7 43.2 44.8 46.4 48.0 49.6 51.2 52.8 54.5 56.1 57.8 59.5 61.2 62.9 64.6 66.3 68.0 69.8 71.5 73.3 75.1 76.9 78.7 80.5 82.430 43.9 45.5 47.1 48.7 50.4 52.0 53.7 55.4 57.1 58.8 60.5 62.2 64.0 65.7 67.5 69.3 71.0 72.9 74.7 76.5 78.3 80.2 82.1 84.0 85.931 46.2 47.9 49.5 51.2 52.9 54.6 56.3 58.1 59.8 61.6 63.3 65.1 66.9 68.7 70.5 72.4 74.2 76.1 78.0 79.9 81.8 83.7 85.7 87.6 89.632 48.7 50.4 52.1 53.8 55.6 57.3 59.1 60.9 62.7 64.5 66.3 68.2 70.0 71.9 73.8 75.7 77.6 79.5 81.5 83.5 85.4 87.5 89.5 91.5 93.633 51.2 53.0 54.8 56.5 58.3 60.2 62.0 63.8 65.7 67.6 69.5 71.4 73.3 75.2 77.2 79.2 81.2 83.2 85.2 87.3 89.3 91.4 93.6 95.7 97.834 53.9 55.7 57.6 59.4 61.3 63.1 65.0 67.0 68.9 70.8 72.8 74.8 76.8 78.8 80.8 82.9 85.0 87.1 89.2 91.4 93.5 95.7 97.9 100.2 102.435 56.8 58.6 60.5 62.4 64.4 66.3 68.3 70.3 72.3 74.3 76.3 78.4 80.5 82.6 84.7 86.9 89.1 91.3 93.5 95.7 98.0 100.3 102.6 105.0 107.336 59.8 61.7 63.7 65.7 67.7 69.7 71.7 73.8 75.9 78.0 80.1 82.3 84.5 86.7 88.9 91.2 93.5 95.8 98.1 100.5 102.9 105.3 107.7 110.2 112.737 62.9 65.0 67.0 69.1 71.2 73.3 75.4 77.6 79.8 82.0 84.2 86.5 88.8 91.1 93.4 95.8 98.2 100.6 103.1 105.6 108.1 110.7 113.3 115.9 118.638 66.3 68.4 70.6 72.7 74.9 77.1 79.4 81.6 83.9 86.2 88.6 91.0 93.4 95.8 98.3 100.8 103.4 105.9 108.6 111.2 113.9 116.6 119.4 122.2 125.039 70.0 72.2 74.4 76.7 78.9 81.3 83.6 86.0 88.4 90.9 93.4 95.9 98.4 101.0 103.6 106.3 109.0 111.8 114.6 117.4 120.3 123.2 126.1 129.2 132.240 73.8 76.2 78.5 80.9 83.3 85.7 88.2 90.8 93.3 95.9 98.5 101.2 103.9 106.7 109.5 112.4 115.3 118.2 121.2 124.3 127.4 130.5 133.7 137.0 140.341 78.0 80.5 83.0 85.5 88.0 90.6 93.3 95.9 98.7 101.4 104.3 107.1 110.0 113.0 116.0 119.1 122.2 125.4 128.7 132.0 135.4 138.8 142.3 145.9 149.542 82.6 85.2 87.8 90.5 93.2 96.0 98.8 101.7 104.6 107.6 110.6 113.7 116.9 120.1 123.4 126.7 130.1 133.6 137.2 140.8 144.5 148.3 152.2 156.1 160.243 87.6 90.4 93.2 96.0 99.0 101.9 105.0 108.1 111.2 114.5 117.8 121.1 124.6 128.1 131.7 135.4 139.1 143.0 147.0 151.0 155.2 159.4 163.8 168.2 172.844 93.1 96.1 99.1 102.2 105.4 108.6 111.9 115.3 118.7 122.3 125.9 129.6 133.4 137.4 141.4 145.5 149.7 154.1 158.5 163.1 167.9 172.7 177.7 182.9 188.245 99.3 102.5 105.8 109.2 112.6 116.2 119.8 123.6 127.4 131.4 135.4 139.6 143.9 148.3 152.9 157.6 162.4 167.4 172.6 178.0 183.5 189.2 195.1 201.2 207.546 106.3 109.8 113.4 117.2 121.0 125.0 129.1 133.3 137.6 142.1 146.7 151.5 156.5 161.6 167.0 172.5 178.2 184.2 190.4 196.8 203.5 210.5 217.8 225.4 233.347 114.3 118.3 122.4 126.6 130.9 135.4 140.1 145.0 150.0 155.3 160.7 166.4 172.3 178.5 185.0 191.8 198.9 206.4 214.2 222.4 231.0 240.0 249.5 259.5 270.048 123.9 128.4 133.1 137.9 143.0 148.3 153.9 159.7 165.8 172.2 178.9 186.0 193.5 201.4 209.8 218.7 228.2 238.2 248.9 260.3 272.3 285.1 298.7 313.0 328.249 135.5 140.8 146.4 152.3 158.5 165.0 172.0 179.3 187.2 195.6 204.6 214.3 224.7 235.9 248.1 261.3 275.5 290.9 307.6 325.5 344.8 365.4 387.3 410.6 435.2

Quanti-Tray and Defined Substrate Technology are trademarks or registered trademarks of IDEXX Laboratories, Inc. in the United States and/or other countries. Covered by U.S. Patent Numbers 4,925,789; 5,429,933; 5,518,892. Other patents pending.
IDEXX Quanti-Tray/2000 MPN Table

# Large 

Wells 

Positive

# Small Wells Positive

25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48

0 25.3 26.4 27.4 28.4 29.5 30.5 31.5 32.6 33.6 34.7 35.7 36.8 37.8 38.9 40.0 41.0 42.1 43.1 44.2 45.3 46.3 47.4 48.5 49.51 26.6 27.7 28.7 29.8 30.8 31.9 32.9 34.0 35.0 36.1 37.2 38.2 39.3 40.4 41.4 42.5 43.6 44.7 45.7 46.8 47.9 49.0 50.1 51.22 27.9 29.0 30.0 31.1 32.2 33.2 34.3 35.4 36.5 37.5 38.6 39.7 40.8 41.9 43.0 44.0 45.1 46.2 47.3 48.4 49.5 50.6 51.7 52.83 29.3 30.4 31.4 32.5 33.6 34.7 35.8 36.8 37.9 39.0 40.1 41.2 42.3 43.4 44.5 45.6 46.7 47.8 48.9 50.0 51.2 52.3 53.4 54.54 30.7 31.8 32.8 33.9 35.0 36.1 37.2 38.3 39.4 40.5 41.6 42.8 43.9 45.0 46.1 47.2 48.3 49.5 50.6 51.7 52.9 54.0 55.1 56.35 32.1 33.2 34.3 35.4 36.5 37.6 38.7 39.9 41.0 42.1 43.2 44.4 45.5 46.6 47.7 48.9 50.0 51.2 52.3 53.5 54.6 55.8 56.9 58.16 33.5 34.7 35.8 36.9 38.0 39.2 40.3 41.4 42.6 43.7 44.8 46.0 47.1 48.3 49.4 50.6 51.7 52.9 54.1 55.2 56.4 57.6 58.7 59.97 35.0 36.2 37.3 38.4 39.6 40.7 41.9 43.0 44.2 45.3 46.5 47.7 48.8 50.0 51.2 52.3 53.5 54.7 55.9 57.1 58.3 59.4 60.6 61.88 36.6 37.7 38.9 40.0 41.2 42.3 43.5 44.7 45.9 47.0 48.2 49.4 50.6 51.8 53.0 54.1 55.3 56.5 57.7 59.0 60.2 61.4 62.6 63.89 38.1 39.3 40.5 41.6 42.8 44.0 45.2 46.4 47.6 48.8 50.0 51.2 52.4 53.6 54.8 56.0 57.2 58.4 59.7 60.9 62.1 63.4 64.6 65.8

10 39.7 40.9 42.1 43.3 44.5 45.7 46.9 48.1 49.3 50.6 51.8 53.0 54.2 55.5 56.7 57.9 59.2 60.4 61.7 62.9 64.2 65.4 66.7 67.9

11 41.4 42.6 43.8 45.0 46.3 47.5 48.7 49.9 51.2 52.4 53.7 54.9 56.1 57.4 58.6 59.9 61.2 62.4 63.7 65.0 66.3 67.5 68.8 70.1

12 43.1 44.3 45.6 46.8 48.1 49.3 50.6 51.8 53.1 54.3 55.6 56.8 58.1 59.4 60.7 62.0 63.2 64.5 65.8 67.1 68.4 69.7 71.0 72.413 44.9 46.1 47.4 48.6 49.9 51.2 52.5 53.7 55.0 56.3 57.6 58.9 60.2 61.5 62.8 64.1 65.4 66.7 68.0 69.3 70.7 72.0 73.3 74.714 46.7 48.0 49.3 50.5 51.8 53.1 54.4 55.7 57.0 58.3 59.6 60.9 62.3 63.6 64.9 66.3 67.6 68.9 70.3 71.6 73.0 74.4 75.7 77.115 48.6 49.9 51.2 52.5 53.8 55.1 56.4 57.8 59.1 60.4 61.8 63.1 64.5 65.8 67.2 68.5 69.9 71.3 72.6 74.0 75.4 76.8 78.2 79.616 50.5 51.8 53.2 54.5 55.8 57.2 58.5 59.9 61.2 62.6 64.0 65.3 66.7 68.1 69.5 70.9 72.3 73.7 75.1 76.5 77.9 79.3 80.8 82.217 52.5 53.9 55.2 56.6 58.0 59.3 60.7 62.1 63.5 64.9 66.3 67.7 69.1 70.5 71.9 73.3 74.8 76.2 77.6 79.1 80.5 82.0 83.5 84.918 54.6 56.0 57.4 58.8 60.2 61.6 63.0 64.4 65.8 67.2 68.6 70.1 71.5 73.0 74.4 75.9 77.3 78.8 80.3 81.8 83.3 84.8 86.3 87.819 56.8 58.2 59.6 61.0 62.4 63.9 65.3 66.8 68.2 69.7 71.1 72.6 74.1 75.5 77.0 78.5 80.0 81.5 83.1 84.6 86.1 87.6 89.2 90.720 59.0 60.4 61.9 63.3 64.8 66.3 67.7 69.2 70.7 72.2 73.7 75.2 76.7 78.2 79.8 81.3 82.8 84.4 85.9 87.5 89.1 90.7 92.2 93.821 61.3 62.8 64.3 65.8 67.3 68.8 70.3 71.8 73.3 74.9 76.4 77.9 79.5 81.1 82.6 84.2 85.8 87.4 89.0 90.6 92.2 93.8 95.4 97.122 63.8 65.3 66.8 68.3 69.8 71.4 72.9 74.5 76.1 77.6 79.2 80.8 82.4 84.0 85.6 87.2 88.9 90.5 92.1 93.8 95.5 97.1 98.8 100.523 66.3 67.8 69.4 71.0 72.5 74.1 75.7 77.3 78.9 80.5 82.2 83.8 85.4 87.1 88.7 90.4 92.1 93.8 95.5 97.2 98.9 100.6 102.4 104.124 68.9 70.5 72.1 73.7 75.3 77.0 78.6 80.3 81.9 83.6 85.2 86.9 88.6 90.3 92.0 93.8 95.5 97.2 99.0 100.7 102.5 104.3 106.1 107.925 71.7 73.3 75.0 76.6 78.3 80.0 81.7 83.3 85.1 86.8 88.5 90.2 92.0 93.7 95.5 97.3 99.1 100.9 102.7 104.5 106.3 108.2 110.0 111.926 74.6 76.3 78.0 79.7 81.4 83.1 84.8 86.6 88.4 90.1 91.9 93.7 95.5 97.3 99.2 101.0 102.9 104.7 106.6 108.5 110.4 112.3 114.2 116.227 77.6 79.4 81.1 82.9 84.6 86.4 88.2 90.0 91.9 93.7 95.5 97.4 99.3 101.2 103.1 105.0 106.9 108.8 110.8 112.7 114.7 116.7 118.7 120.728 80.8 82.6 84.4 86.3 88.1 89.9 91.8 93.7 95.6 97.5 99.4 101.3 103.3 105.2 107.2 109.2 111.2 113.2 115.2 117.3 119.3 121.4 123.5 125.629 84.2 86.1 87.9 89.8 91.7 93.7 95.6 97.5 99.5 101.5 103.5 105.5 107.5 109.5 111.6 113.7 115.7 117.8 120.0 122.1 124.2 126.4 128.6 130.830 87.8 89.7 91.7 93.6 95.6 97.6 99.6 101.6 103.7 105.7 107.8 109.9 112.0 114.2 116.3 118.5 120.6 122.8 125.1 127.3 129.5 131.8 134.1 136.431 91.6 93.6 95.6 97.7 99.7 101.8 103.9 106.0 108.2 110.3 112.5 114.7 116.9 119.1 121.4 123.6 125.9 128.2 130.5 132.9 135.3 137.7 140.1 142.532 95.7 97.8 99.9 102.0 104.2 106.3 108.5 110.7 113.0 115.2 117.5 119.8 122.1 124.5 126.8 129.2 131.6 134.0 136.5 139.0 141.5 144.0 146.6 149.133 100.0 102.2 104.4 106.6 108.9 111.2 113.5 115.8 118.2 120.5 122.9 125.4 127.8 130.3 132.8 135.3 137.8 140.4 143.0 145.6 148.3 150.9 153.7 156.434 104.7 107.0 109.3 111.7 114.0 116.4 118.9 121.3 123.8 126.3 128.8 131.4 134.0 136.6 139.2 141.9 144.6 147.4 150.1 152.9 155.7 158.6 161.5 164.435 109.7 112.2 114.6 117.1 119.6 122.2 124.7 127.3 129.9 132.6 135.3 138.0 140.8 143.6 146.4 149.2 152.1 155.0 158.0 161.0 164.0 167.1 170.2 173.336 115.2 117.8 120.4 123.0 125.7 128.4 131.1 133.9 136.7 139.5 142.4 145.3 148.3 151.3 154.3 157.3 160.5 163.6 166.8 170.0 173.3 176.6 179.9 183.337 121.3 124.0 126.8 129.6 132.4 135.3 138.2 141.2 144.2 147.3 150.3 153.5 156.7 159.9 163.1 166.5 169.8 173.2 176.7 180.2 183.7 187.3 191.0 194.738 127.9 130.8 133.8 136.8 139.9 143.0 146.2 149.4 152.6 155.9 159.2 162.6 166.1 169.6 173.2 176.8 180.4 184.2 188.0 191.8 195.7 199.7 203.7 207.739 135.3 138.5 141.7 145.0 148.3 151.7 155.1 158.6 162.1 165.7 169.4 173.1 176.9 180.7 184.7 188.7 192.7 196.8 201.0 205.3 209.6 214.0 218.5 223.040 143.7 147.1 150.6 154.2 157.8 161.5 165.3 169.1 173.0 177.0 181.1 185.2 189.4 193.7 198.1 202.5 207.1 211.7 216.4 221.1 226.0 231.0 236.0 241.141 153.2 157.0 160.9 164.8 168.9 173.0 177.2 181.5 185.8 190.3 194.8 199.5 204.2 209.1 214.0 219.1 224.2 229.4 234.8 240.2 245.8 251.5 257.2 263.142 164.3 168.6 172.9 177.3 181.9 186.5 191.3 196.1 201.1 206.2 211.4 216.7 222.2 227.7 233.4 239.2 245.2 251.3 257.5 263.8 270.3 276.9 283.6 290.543 177.5 182.3 187.3 192.4 197.6 202.9 208.4 214.0 219.8 225.8 231.8 238.1 244.5 251.0 257.7 264.6 271.7 278.9 286.3 293.8 301.5 309.4 317.4 325.744 193.6 199.3 205.1 211.0 217.2 223.5 230.0 236.7 243.6 250.8 258.1 265.6 273.3 281.2 289.4 297.8 306.3 315.1 324.1 333.3 342.8 352.4 362.3 372.445 214.1 220.9 227.9 235.2 242.7 250.4 258.4 266.7 275.3 284.1 293.3 302.6 312.3 322.3 332.5 343.0 353.8 364.9 376.2 387.9 399.8 412.0 424.5 437.446 241.5 250.0 258.9 268.2 277.8 287.8 298.1 308.8 319.9 331.4 343.3 355.5 368.1 381.1 394.5 408.3 422.5 437.1 452.0 467.4 483.3 499.6 516.3 533.547 280.9 292.4 304.4 316.9 330.0 343.6 357.8 372.5 387.7 403.4 419.8 436.6 454.1 472.1 490.7 509.9 529.8 550.4 571.7 593.8 616.7 640.5 665.3 691.048 344.1 360.9 378.4 396.8 416.0 436.0 456.9 478.6 501.2 524.7 549.3 574.8 601.5 629.4 658.6 689.3 721.5 755.6 791.5 829.7 870.4 913.9 960.6 1011.249 461.1 488.4 517.2 547.5 579.4 613.1 648.8 686.7 727.0 770.1 816.4 866.4 920.8 980.4 1046.2 1119.9 1203.3 1299.7 1413.6 1553.1 1732.9 1986.3 2419.6 >2419.6
IDEXX Quanti-Tray/2000

Version Française

Quanti-Tray et Defined Substrate Technology sont des marques de fabrique ou des marques déposées 

d'IDEXX Laboratories, Inc. aux États-Unis et/ou dans d'autres pays. Protégé par les brevets américains 

n° 5,518,892; 5,620,895; and 5,753,456.. Autres brevets en cours d'enregistrement.

Introduction

Les plateaux Quanti-Tray/2000 d’IDEXX, spécialement conçus pour effectuer des numérations bactériennes sur des échantillons de 100 

millilitres, font appel à la technologie Defined Substrate Technology d’IDEXX basée sur l’utilisation de réactifs. Versez le mélange réactif/

échantillon dans un Quanti-Tray/2000, placez ensuite le tout dans le Quanti-Tray Sealer et procédez à l’incubation du mélange selon 

les instructions accompagnant les réactifs. Comptez ensuite le nombre de cupules positives et référez-vous à la table ci-dessous pour 

déterminer le nombre le plus probable (NPP).

Contentu

Cette boîte contient Quanti-Tray/2000 stériles.

Services techniques IDEXX

00800-4339-9111

www.idexx.fr/eau

Mode d'emploi

1. Prenez un Quanti-Tray et tenez-le à 

la verticale en veillant à orienter les 

cupules vers la paume de la main.

2. Appuyez sur la partie supérieure du 

Quanti-Tray afin qu’il vienne se loger 

dans la paume de la main.

3. Ouvrez le Quanti-Tray en retirant 

la feuille en aluminium. Evitez tout 

contact avec la partie intérieure de 

l’aluminium ou du plateau.

4. Versez directement le mélange réactif/

échantillon dans le Quanti-Tray en 

évitant tout contact avec la languette 

d’aluminium. Tapoter les petits puits 2 

à 3 fois afin de libérer les bulles d'air. 

Laisser la mousse se dissiper.

5. Placez le Quanti-Tray rempli 

d’échantillons sur le porte-plateau en 

caoutchouc du Quanti-Tray Sealer en 

veillant à orienter les cupules (parties 

en plastique) vers le bas afin qu’elles 

viennent s’encastrer parfaitement 

dans le porte-plateau.

6. Scellez le Quanti-Tray conformément 

aux instructions accompagnant 

le Sealer.

7. Incubez conformément aux 

instructions accompagnant le réactif.

8. Comptez le nombre de cupules 

positives, puis référez-vous à la table 

NPP au verso pour déterminer le 

nombre le plus probable (NPP).

9. Débarrassez-vous du milieu en suivant 

les bonnes pratiques de laboratoire.

IDEXX Laboratories, Inc.

One IDEXX Drive

Westbrook, Maine 04092 USA
Table NPP IDEXX Quanti-Tray/2000

# Grands 

Puits 

Positifs

# Petits Puits Positifs

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

0 <1 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.1 15.1 16.1 17.1 18.1 19.1 20.2 21.2 22.2 23.3 24.31 1.0 2.0 3.0 4.0 5.0 6.0 7.1 8.1 9.1 10.1 11.1 12.1 13.2 14.2 15.2 16.2 17.3 18.3 19.3 20.4 21.4 22.4 23.5 24.5 25.62 2.0 3.0 4.1 5.1 6.1 7.1 8.1 9.2 10.2 11.2 12.2 13.3 14.3 15.4 16.4 17.4 18.5 19.5 20.6 21.6 22.7 23.7 24.8 25.8 26.93 3.1 4.1 5.1 6.1 7.2 8.2 9.2 10.3 11.3 12.4 13.4 14.5 15.5 16.5 17.6 18.6 19.7 20.8 21.8 22.9 23.9 25.0 26.1 27.1 28.24 4.1 5.2 6.2 7.2 8.3 9.3 10.4 11.4 12.5 13.5 14.6 15.6 16.7 17.8 18.8 19.9 21.0 22.0 23.1 24.2 25.3 26.3 27.4 28.5 29.65 5.2 6.3 7.3 8.4 9.4 10.5 11.5 12.6 13.7 14.7 15.8 16.9 17.9 19.0 20.1 21.2 22.2 23.3 24.4 25.5 26.6 27.7 28.8 29.9 31.06 6.3 7.4 8.4 9.5 10.6 11.6 12.7 13.8 14.9 16.0 17.0 18.1 19.2 20.3 21.4 22.5 23.6 24.7 25.8 26.9 28.0 29.1 30.2 31.3 32.47 7.5 8.5 9.6 10.7 11.8 12.8 13.9 15.0 16.1 17.2 18.3 19.4 20.5 21.6 22.7 23.8 24.9 26.0 27.1 28.3 29.4 30.5 31.6 32.8 33.98 8.6 9.7 10.8 11.9 13.0 14.1 15.2 16.3 17.4 18.5 19.6 20.7 21.8 22.9 24.1 25.2 26.3 27.4 28.6 29.7 30.8 32.0 33.1 34.3 35.49 9.8 10.9 12.0 13.1 14.2 15.3 16.4 17.6 18.7 19.8 20.9 22.0 23.2 24.3 25.4 26.6 27.7 28.9 30.0 31.2 32.3 33.5 34.6 35.8 37.0

10 11.0 12.1 13.2 14.4 15.5 16.6 17.7 18.9 20.0 21.1 22.3 23.4 24.6 25.7 26.9 28.0 29.2 30.3 31.5 32.7 33.8 35.0 36.2 37.4 38.6

11 12.2 13.4 14.5 15.6 16.8 17.9 19.1 20.2 21.4 22.5 23.7 24.8 26.0 27.2 28.3 29.5 30.7 31.9 33.0 34.2 35.4 36.6 37.8 39.0 40.2

12 13.5 14.6 15.8 16.9 18.1 19.3 20.4 21.6 22.8 23.9 25.1 26.3 27.5 28.6 29.8 31.0 32.2 33.4 34.6 35.8 37.0 38.2 39.5 40.7 41.913 14.8 16.0 17.1 18.3 19.5 20.6 21.8 23.0 24.2 25.4 26.6 27.8 29.0 30.2 31.4 32.6 33.8 35.0 36.2 37.5 38.7 39.9 41.2 42.4 43.614 16.1 17.3 18.5 19.7 20.9 22.1 23.3 24.5 25.7 26.9 28.1 29.3 30.5 31.7 33.0 34.2 35.4 36.7 37.9 39.1 40.4 41.6 42.9 44.2 45.415 17.5 18.7 19.9 21.1 22.3 23.5 24.7 25.9 27.2 28.4 29.6 30.9 32.1 33.3 34.6 35.8 37.1 38.4 39.6 40.9 42.2 43.4 44.7 46.0 47.316 18.9 20.1 21.3 22.6 23.8 25.0 26.2 27.5 28.7 30.0 31.2 32.5 33.7 35.0 36.3 37.5 38.8 40.1 41.4 42.7 44.0 45.3 46.6 47.9 49.217 20.3 21.6 22.8 24.1 25.3 26.6 27.8 29.1 30.3 31.6 32.9 34.1 35.4 36.7 38.0 39.3 40.6 41.9 43.2 44.5 45.9 47.2 48.5 49.8 51.218 21.8 23.1 24.3 25.6 26.9 28.1 29.4 30.7 32.0 33.3 34.6 35.9 37.2 38.5 39.8 41.1 42.4 43.8 45.1 46.5 47.8 49.2 50.5 51.9 53.219 23.3 24.6 25.9 27.2 28.5 29.8 31.1 32.4 33.7 35.0 36.3 37.6 39.0 40.3 41.6 43.0 44.3 45.7 47.1 48.4 49.8 51.2 52.6 54.0 55.420 24.9 26.2 27.5 28.8 30.1 31.5 32.8 34.1 35.4 36.8 38.1 39.5 40.8 42.2 43.6 44.9 46.3 47.7 49.1 50.5 51.9 53.3 54.7 56.1 57.621 26.5 27.9 29.2 30.5 31.8 33.2 34.5 35.9 37.3 38.6 40.0 41.4 42.8 44.1 45.5 46.9 48.4 49.8 51.2 52.6 54.1 55.5 56.9 58.4 59.922 28.2 29.5 30.9 32.3 33.6 35.0 36.4 37.7 39.1 40.5 41.9 43.3 44.8 46.2 47.6 49.0 50.5 51.9 53.4 54.8 56.3 57.8 59.3 60.8 62.323 29.9 31.3 32.7 34.1 35.5 36.8 38.3 39.7 41.1 42.5 43.9 45.4 46.8 48.3 49.7 51.2 52.7 54.2 55.6 57.1 58.6 60.2 61.7 63.2 64.724 31.7 33.1 34.5 35.9 37.3 38.8 40.2 41.7 43.1 44.6 46.0 47.5 49.0 50.5 52.0 53.5 55.0 56.5 58.0 59.5 61.1 62.6 64.2 65.8 67.325 33.6 35.0 36.4 37.9 39.3 40.8 42.2 43.7 45.2 46.7 48.2 49.7 51.2 52.7 54.3 55.8 57.3 58.9 60.5 62.0 63.6 65.2 66.8 68.4 70.026 35.5 36.9 38.4 39.9 41.4 42.8 44.3 45.9 47.4 48.9 50.4 52.0 53.5 55.1 56.7 58.2 59.8 61.4 63.0 64.7 66.3 67.9 69.6 71.2 72.927 37.4 38.9 40.4 42.0 43.5 45.0 46.5 48.1 49.6 51.2 52.8 54.4 56.0 57.6 59.2 60.8 62.4 64.1 65.7 67.4 69.1 70.8 72.5 74.2 75.928 39.5 41.0 42.6 44.1 45.7 47.3 48.8 50.4 52.0 53.6 55.2 56.9 58.5 60.2 61.8 63.5 65.2 66.9 68.6 70.3 72.0 73.7 75.5 77.3 79.029 41.7 43.2 44.8 46.4 48.0 49.6 51.2 52.8 54.5 56.1 57.8 59.5 61.2 62.9 64.6 66.3 68.0 69.8 71.5 73.3 75.1 76.9 78.7 80.5 82.430 43.9 45.5 47.1 48.7 50.4 52.0 53.7 55.4 57.1 58.8 60.5 62.2 64.0 65.7 67.5 69.3 71.0 72.9 74.7 76.5 78.3 80.2 82.1 84.0 85.931 46.2 47.9 49.5 51.2 52.9 54.6 56.3 58.1 59.8 61.6 63.3 65.1 66.9 68.7 70.5 72.4 74.2 76.1 78.0 79.9 81.8 83.7 85.7 87.6 89.632 48.7 50.4 52.1 53.8 55.6 57.3 59.1 60.9 62.7 64.5 66.3 68.2 70.0 71.9 73.8 75.7 77.6 79.5 81.5 83.5 85.4 87.5 89.5 91.5 93.633 51.2 53.0 54.8 56.5 58.3 60.2 62.0 63.8 65.7 67.6 69.5 71.4 73.3 75.2 77.2 79.2 81.2 83.2 85.2 87.3 89.3 91.4 93.6 95.7 97.834 53.9 55.7 57.6 59.4 61.3 63.1 65.0 67.0 68.9 70.8 72.8 74.8 76.8 78.8 80.8 82.9 85.0 87.1 89.2 91.4 93.5 95.7 97.9 100.2 102.435 56.8 58.6 60.5 62.4 64.4 66.3 68.3 70.3 72.3 74.3 76.3 78.4 80.5 82.6 84.7 86.9 89.1 91.3 93.5 95.7 98.0 100.3 102.6 105.0 107.336 59.8 61.7 63.7 65.7 67.7 69.7 71.7 73.8 75.9 78.0 80.1 82.3 84.5 86.7 88.9 91.2 93.5 95.8 98.1 100.5 102.9 105.3 107.7 110.2 112.737 62.9 65.0 67.0 69.1 71.2 73.3 75.4 77.6 79.8 82.0 84.2 86.5 88.8 91.1 93.4 95.8 98.2 100.6 103.1 105.6 108.1 110.7 113.3 115.9 118.638 66.3 68.4 70.6 72.7 74.9 77.1 79.4 81.6 83.9 86.2 88.6 91.0 93.4 95.8 98.3 100.8 103.4 105.9 108.6 111.2 113.9 116.6 119.4 122.2 125.039 70.0 72.2 74.4 76.7 78.9 81.3 83.6 86.0 88.4 90.9 93.4 95.9 98.4 101.0 103.6 106.3 109.0 111.8 114.6 117.4 120.3 123.2 126.1 129.2 132.240 73.8 76.2 78.5 80.9 83.3 85.7 88.2 90.8 93.3 95.9 98.5 101.2 103.9 106.7 109.5 112.4 115.3 118.2 121.2 124.3 127.4 130.5 133.7 137.0 140.341 78.0 80.5 83.0 85.5 88.0 90.6 93.3 95.9 98.7 101.4 104.3 107.1 110.0 113.0 116.0 119.1 122.2 125.4 128.7 132.0 135.4 138.8 142.3 145.9 149.542 82.6 85.2 87.8 90.5 93.2 96.0 98.8 101.7 104.6 107.6 110.6 113.7 116.9 120.1 123.4 126.7 130.1 133.6 137.2 140.8 144.5 148.3 152.2 156.1 160.243 87.6 90.4 93.2 96.0 99.0 101.9 105.0 108.1 111.2 114.5 117.8 121.1 124.6 128.1 131.7 135.4 139.1 143.0 147.0 151.0 155.2 159.4 163.8 168.2 172.844 93.1 96.1 99.1 102.2 105.4 108.6 111.9 115.3 118.7 122.3 125.9 129.6 133.4 137.4 141.4 145.5 149.7 154.1 158.5 163.1 167.9 172.7 177.7 182.9 188.245 99.3 102.5 105.8 109.2 112.6 116.2 119.8 123.6 127.4 131.4 135.4 139.6 143.9 148.3 152.9 157.6 162.4 167.4 172.6 178.0 183.5 189.2 195.1 201.2 207.546 106.3 109.8 113.4 117.2 121.0 125.0 129.1 133.3 137.6 142.1 146.7 151.5 156.5 161.6 167.0 172.5 178.2 184.2 190.4 196.8 203.5 210.5 217.8 225.4 233.347 114.3 118.3 122.4 126.6 130.9 135.4 140.1 145.0 150.0 155.3 160.7 166.4 172.3 178.5 185.0 191.8 198.9 206.4 214.2 222.4 231.0 240.0 249.5 259.5 270.048 123.9 128.4 133.1 137.9 143.0 148.3 153.9 159.7 165.8 172.2 178.9 186.0 193.5 201.4 209.8 218.7 228.2 238.2 248.9 260.3 272.3 285.1 298.7 313.0 328.249 135.5 140.8 146.4 152.3 158.5 165.0 172.0 179.3 187.2 195.6 204.6 214.3 224.7 235.9 248.1 261.3 275.5 290.9 307.6 325.5 344.8 365.4 387.3 410.6 435.2

Quanti-Tray et Defined Substrate Technology sont des marques de fabrique ou des marques déposées d'IDEXX Laboratories, Inc. aux États-Unis et/ou dans d'autres pays. Protégé par les brevets américains n° 4,925,789; 5,429,933; 5,518,892. Autres brevets en cours d'enregistrement.
Table NPP IDEXX Quanti-Tray/2000

# Grands 

Puits 

Positifs

# Petits Puits Positifs

25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48

0 25.3 26.4 27.4 28.4 29.5 30.5 31.5 32.6 33.6 34.7 35.7 36.8 37.8 38.9 40.0 41.0 42.1 43.1 44.2 45.3 46.3 47.4 48.5 49.51 26.6 27.7 28.7 29.8 30.8 31.9 32.9 34.0 35.0 36.1 37.2 38.2 39.3 40.4 41.4 42.5 43.6 44.7 45.7 46.8 47.9 49.0 50.1 51.22 27.9 29.0 30.0 31.1 32.2 33.2 34.3 35.4 36.5 37.5 38.6 39.7 40.8 41.9 43.0 44.0 45.1 46.2 47.3 48.4 49.5 50.6 51.7 52.83 29.3 30.4 31.4 32.5 33.6 34.7 35.8 36.8 37.9 39.0 40.1 41.2 42.3 43.4 44.5 45.6 46.7 47.8 48.9 50.0 51.2 52.3 53.4 54.54 30.7 31.8 32.8 33.9 35.0 36.1 37.2 38.3 39.4 40.5 41.6 42.8 43.9 45.0 46.1 47.2 48.3 49.5 50.6 51.7 52.9 54.0 55.1 56.35 32.1 33.2 34.3 35.4 36.5 37.6 38.7 39.9 41.0 42.1 43.2 44.4 45.5 46.6 47.7 48.9 50.0 51.2 52.3 53.5 54.6 55.8 56.9 58.16 33.5 34.7 35.8 36.9 38.0 39.2 40.3 41.4 42.6 43.7 44.8 46.0 47.1 48.3 49.4 50.6 51.7 52.9 54.1 55.2 56.4 57.6 58.7 59.97 35.0 36.2 37.3 38.4 39.6 40.7 41.9 43.0 44.2 45.3 46.5 47.7 48.8 50.0 51.2 52.3 53.5 54.7 55.9 57.1 58.3 59.4 60.6 61.88 36.6 37.7 38.9 40.0 41.2 42.3 43.5 44.7 45.9 47.0 48.2 49.4 50.6 51.8 53.0 54.1 55.3 56.5 57.7 59.0 60.2 61.4 62.6 63.89 38.1 39.3 40.5 41.6 42.8 44.0 45.2 46.4 47.6 48.8 50.0 51.2 52.4 53.6 54.8 56.0 57.2 58.4 59.7 60.9 62.1 63.4 64.6 65.8

10 39.7 40.9 42.1 43.3 44.5 45.7 46.9 48.1 49.3 50.6 51.8 53.0 54.2 55.5 56.7 57.9 59.2 60.4 61.7 62.9 64.2 65.4 66.7 67.9

11 41.4 42.6 43.8 45.0 46.3 47.5 48.7 49.9 51.2 52.4 53.7 54.9 56.1 57.4 58.6 59.9 61.2 62.4 63.7 65.0 66.3 67.5 68.8 70.1

12 43.1 44.3 45.6 46.8 48.1 49.3 50.6 51.8 53.1 54.3 55.6 56.8 58.1 59.4 60.7 62.0 63.2 64.5 65.8 67.1 68.4 69.7 71.0 72.413 44.9 46.1 47.4 48.6 49.9 51.2 52.5 53.7 55.0 56.3 57.6 58.9 60.2 61.5 62.8 64.1 65.4 66.7 68.0 69.3 70.7 72.0 73.3 74.714 46.7 48.0 49.3 50.5 51.8 53.1 54.4 55.7 57.0 58.3 59.6 60.9 62.3 63.6 64.9 66.3 67.6 68.9 70.3 71.6 73.0 74.4 75.7 77.115 48.6 49.9 51.2 52.5 53.8 55.1 56.4 57.8 59.1 60.4 61.8 63.1 64.5 65.8 67.2 68.5 69.9 71.3 72.6 74.0 75.4 76.8 78.2 79.616 50.5 51.8 53.2 54.5 55.8 57.2 58.5 59.9 61.2 62.6 64.0 65.3 66.7 68.1 69.5 70.9 72.3 73.7 75.1 76.5 77.9 79.3 80.8 82.217 52.5 53.9 55.2 56.6 58.0 59.3 60.7 62.1 63.5 64.9 66.3 67.7 69.1 70.5 71.9 73.3 74.8 76.2 77.6 79.1 80.5 82.0 83.5 84.918 54.6 56.0 57.4 58.8 60.2 61.6 63.0 64.4 65.8 67.2 68.6 70.1 71.5 73.0 74.4 75.9 77.3 78.8 80.3 81.8 83.3 84.8 86.3 87.819 56.8 58.2 59.6 61.0 62.4 63.9 65.3 66.8 68.2 69.7 71.1 72.6 74.1 75.5 77.0 78.5 80.0 81.5 83.1 84.6 86.1 87.6 89.2 90.720 59.0 60.4 61.9 63.3 64.8 66.3 67.7 69.2 70.7 72.2 73.7 75.2 76.7 78.2 79.8 81.3 82.8 84.4 85.9 87.5 89.1 90.7 92.2 93.821 61.3 62.8 64.3 65.8 67.3 68.8 70.3 71.8 73.3 74.9 76.4 77.9 79.5 81.1 82.6 84.2 85.8 87.4 89.0 90.6 92.2 93.8 95.4 97.122 63.8 65.3 66.8 68.3 69.8 71.4 72.9 74.5 76.1 77.6 79.2 80.8 82.4 84.0 85.6 87.2 88.9 90.5 92.1 93.8 95.5 97.1 98.8 100.523 66.3 67.8 69.4 71.0 72.5 74.1 75.7 77.3 78.9 80.5 82.2 83.8 85.4 87.1 88.7 90.4 92.1 93.8 95.5 97.2 98.9 100.6 102.4 104.124 68.9 70.5 72.1 73.7 75.3 77.0 78.6 80.3 81.9 83.6 85.2 86.9 88.6 90.3 92.0 93.8 95.5 97.2 99.0 100.7 102.5 104.3 106.1 107.925 71.7 73.3 75.0 76.6 78.3 80.0 81.7 83.3 85.1 86.8 88.5 90.2 92.0 93.7 95.5 97.3 99.1 100.9 102.7 104.5 106.3 108.2 110.0 111.926 74.6 76.3 78.0 79.7 81.4 83.1 84.8 86.6 88.4 90.1 91.9 93.7 95.5 97.3 99.2 101.0 102.9 104.7 106.6 108.5 110.4 112.3 114.2 116.227 77.6 79.4 81.1 82.9 84.6 86.4 88.2 90.0 91.9 93.7 95.5 97.4 99.3 101.2 103.1 105.0 106.9 108.8 110.8 112.7 114.7 116.7 118.7 120.728 80.8 82.6 84.4 86.3 88.1 89.9 91.8 93.7 95.6 97.5 99.4 101.3 103.3 105.2 107.2 109.2 111.2 113.2 115.2 117.3 119.3 121.4 123.5 125.629 84.2 86.1 87.9 89.8 91.7 93.7 95.6 97.5 99.5 101.5 103.5 105.5 107.5 109.5 111.6 113.7 115.7 117.8 120.0 122.1 124.2 126.4 128.6 130.830 87.8 89.7 91.7 93.6 95.6 97.6 99.6 101.6 103.7 105.7 107.8 109.9 112.0 114.2 116.3 118.5 120.6 122.8 125.1 127.3 129.5 131.8 134.1 136.431 91.6 93.6 95.6 97.7 99.7 101.8 103.9 106.0 108.2 110.3 112.5 114.7 116.9 119.1 121.4 123.6 125.9 128.2 130.5 132.9 135.3 137.7 140.1 142.532 95.7 97.8 99.9 102.0 104.2 106.3 108.5 110.7 113.0 115.2 117.5 119.8 122.1 124.5 126.8 129.2 131.6 134.0 136.5 139.0 141.5 144.0 146.6 149.133 100.0 102.2 104.4 106.6 108.9 111.2 113.5 115.8 118.2 120.5 122.9 125.4 127.8 130.3 132.8 135.3 137.8 140.4 143.0 145.6 148.3 150.9 153.7 156.434 104.7 107.0 109.3 111.7 114.0 116.4 118.9 121.3 123.8 126.3 128.8 131.4 134.0 136.6 139.2 141.9 144.6 147.4 150.1 152.9 155.7 158.6 161.5 164.435 109.7 112.2 114.6 117.1 119.6 122.2 124.7 127.3 129.9 132.6 135.3 138.0 140.8 143.6 146.4 149.2 152.1 155.0 158.0 161.0 164.0 167.1 170.2 173.336 115.2 117.8 120.4 123.0 125.7 128.4 131.1 133.9 136.7 139.5 142.4 145.3 148.3 151.3 154.3 157.3 160.5 163.6 166.8 170.0 173.3 176.6 179.9 183.337 121.3 124.0 126.8 129.6 132.4 135.3 138.2 141.2 144.2 147.3 150.3 153.5 156.7 159.9 163.1 166.5 169.8 173.2 176.7 180.2 183.7 187.3 191.0 194.738 127.9 130.8 133.8 136.8 139.9 143.0 146.2 149.4 152.6 155.9 159.2 162.6 166.1 169.6 173.2 176.8 180.4 184.2 188.0 191.8 195.7 199.7 203.7 207.739 135.3 138.5 141.7 145.0 148.3 151.7 155.1 158.6 162.1 165.7 169.4 173.1 176.9 180.7 184.7 188.7 192.7 196.8 201.0 205.3 209.6 214.0 218.5 223.040 143.7 147.1 150.6 154.2 157.8 161.5 165.3 169.1 173.0 177.0 181.1 185.2 189.4 193.7 198.1 202.5 207.1 211.7 216.4 221.1 226.0 231.0 236.0 241.141 153.2 157.0 160.9 164.8 168.9 173.0 177.2 181.5 185.8 190.3 194.8 199.5 204.2 209.1 214.0 219.1 224.2 229.4 234.8 240.2 245.8 251.5 257.2 263.142 164.3 168.6 172.9 177.3 181.9 186.5 191.3 196.1 201.1 206.2 211.4 216.7 222.2 227.7 233.4 239.2 245.2 251.3 257.5 263.8 270.3 276.9 283.6 290.543 177.5 182.3 187.3 192.4 197.6 202.9 208.4 214.0 219.8 225.8 231.8 238.1 244.5 251.0 257.7 264.6 271.7 278.9 286.3 293.8 301.5 309.4 317.4 325.744 193.6 199.3 205.1 211.0 217.2 223.5 230.0 236.7 243.6 250.8 258.1 265.6 273.3 281.2 289.4 297.8 306.3 315.1 324.1 333.3 342.8 352.4 362.3 372.445 214.1 220.9 227.9 235.2 242.7 250.4 258.4 266.7 275.3 284.1 293.3 302.6 312.3 322.3 332.5 343.0 353.8 364.9 376.2 387.9 399.8 412.0 424.5 437.446 241.5 250.0 258.9 268.2 277.8 287.8 298.1 308.8 319.9 331.4 343.3 355.5 368.1 381.1 394.5 408.3 422.5 437.1 452.0 467.4 483.3 499.6 516.3 533.547 280.9 292.4 304.4 316.9 330.0 343.6 357.8 372.5 387.7 403.4 419.8 436.6 454.1 472.1 490.7 509.9 529.8 550.4 571.7 593.8 616.7 640.5 665.3 691.048 344.1 360.9 378.4 396.8 416.0 436.0 456.9 478.6 501.2 524.7 549.3 574.8 601.5 629.4 658.6 689.3 721.5 755.6 791.5 829.7 870.4 913.9 960.6 1011.249 461.1 488.4 517.2 547.5 579.4 613.1 648.8 686.7 727.0 770.1 816.4 866.4 920.8 980.4 1046.2 1119.9 1203.3 1299.7 1413.6 1553.1 1732.9 1986.3 2419.6 >2419.6
IDEXX Quanti-Tray/2000

Version Italiano

Quanti-Tray e Defined Substrate Technology sono marchi di proprietà di, e/o registrati da, IDEXX Laboratories, Inc. 

e protetti negli Stati Uniti e/o in altri paesi. Protetto dai brevetti degli U.S.A. nn. 5,518,892; 5,620,895; and 5,753,456. 

Altre domande di brevetto pendenti.

Introduzione

Quanti-Tray/2000 IDEXX sono concepiti per fornire la densità batterica (quantitativa) di campioni da 100 mL utilizzando reagenti Defined 

Substrate Technology IDEXX. Aggiungere la miscela di reagente/campione ad un Quanti-Tray/2000, inserire quest’ultimo nel Quanti-Tray 

Sealer ed incubare in conformità alle istruzioni fornite con il reagente. Contare quindi il numero di pozzetti positivi e, con l’ausilio della 

tabella MPN allegata, determinare il numero più probabile (MPN).

Contenuto

Questa confezione contiene Quanti-Tray/2000 sterili.

Supporto Tecnico IDEXX

00800-4339-9111

www.idexx.it/acqua

Istruzioni per l’uso

1. Con una mano, tenere il Quanti-Tray 

in posizione verticale, con il lato dei 

pozzetti rivolto verso il palmo.

2. Comprimere la parte superiore del 

Quanti-Tray in modo da fletterlo verso 

il palmo.

3. Aprire il Quanti-Tray tirando la linguetta 

in alluminio in direzione opposta al lato 

dei pozzetti. Evitare di toccare la parte 

interna del foglio protettivo in alluminio 

o del dispositivo a pozzetti.

4. Versare direttamente la miscela di 

reagente/campione nel Quanti-Tray, 

evitando di toccare la linguetta in 

alluminio. Dare 2–3 leggeri colpi ai 

pozzetti piccoli per eliminare eventuali 

bolle d'aria. Consentire alla schiuma di 

sedimentare.

5. Una volta versata la miscela, collocare 

il Quanti-Tray nel portapozzetti in 

gomma del Quanti-Tray Sealer, con 

il lto dei pozzetti (in plastica) rivolto 

verso il basso, in modo da assicurarne 

il corretto posizionamento.

6. Sigillare secondo le istruzioni del 

Quanti-Tray Sealer.

7. Incubare secondo le istruzioni fornite 

con il reagente.

8. Contare i pozzetti positivi e consultare 

la tabella MPN sul retro del foglio delle 

istruzioni, per individuare il numero più 

probabile (MPN) corretto.

9. Eliminare el terreno in accordo alle 

Buone pratiche di laboratorio.

IDEXX Laboratories, Inc.

One IDEXX Drive

Westbrook, Maine 04092 USA
IDEXX Quanti-Tray/2000 Tabella MPN

# Pozzetti 

Grande 

Positivi

# Pozzetti Piccoli Positivi

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

0 <1 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.1 15.1 16.1 17.1 18.1 19.1 20.2 21.2 22.2 23.3 24.31 1.0 2.0 3.0 4.0 5.0 6.0 7.1 8.1 9.1 10.1 11.1 12.1 13.2 14.2 15.2 16.2 17.3 18.3 19.3 20.4 21.4 22.4 23.5 24.5 25.62 2.0 3.0 4.1 5.1 6.1 7.1 8.1 9.2 10.2 11.2 12.2 13.3 14.3 15.4 16.4 17.4 18.5 19.5 20.6 21.6 22.7 23.7 24.8 25.8 26.93 3.1 4.1 5.1 6.1 7.2 8.2 9.2 10.3 11.3 12.4 13.4 14.5 15.5 16.5 17.6 18.6 19.7 20.8 21.8 22.9 23.9 25.0 26.1 27.1 28.24 4.1 5.2 6.2 7.2 8.3 9.3 10.4 11.4 12.5 13.5 14.6 15.6 16.7 17.8 18.8 19.9 21.0 22.0 23.1 24.2 25.3 26.3 27.4 28.5 29.65 5.2 6.3 7.3 8.4 9.4 10.5 11.5 12.6 13.7 14.7 15.8 16.9 17.9 19.0 20.1 21.2 22.2 23.3 24.4 25.5 26.6 27.7 28.8 29.9 31.06 6.3 7.4 8.4 9.5 10.6 11.6 12.7 13.8 14.9 16.0 17.0 18.1 19.2 20.3 21.4 22.5 23.6 24.7 25.8 26.9 28.0 29.1 30.2 31.3 32.47 7.5 8.5 9.6 10.7 11.8 12.8 13.9 15.0 16.1 17.2 18.3 19.4 20.5 21.6 22.7 23.8 24.9 26.0 27.1 28.3 29.4 30.5 31.6 32.8 33.98 8.6 9.7 10.8 11.9 13.0 14.1 15.2 16.3 17.4 18.5 19.6 20.7 21.8 22.9 24.1 25.2 26.3 27.4 28.6 29.7 30.8 32.0 33.1 34.3 35.49 9.8 10.9 12.0 13.1 14.2 15.3 16.4 17.6 18.7 19.8 20.9 22.0 23.2 24.3 25.4 26.6 27.7 28.9 30.0 31.2 32.3 33.5 34.6 35.8 37.0

10 11.0 12.1 13.2 14.4 15.5 16.6 17.7 18.9 20.0 21.1 22.3 23.4 24.6 25.7 26.9 28.0 29.2 30.3 31.5 32.7 33.8 35.0 36.2 37.4 38.6

11 12.2 13.4 14.5 15.6 16.8 17.9 19.1 20.2 21.4 22.5 23.7 24.8 26.0 27.2 28.3 29.5 30.7 31.9 33.0 34.2 35.4 36.6 37.8 39.0 40.2

12 13.5 14.6 15.8 16.9 18.1 19.3 20.4 21.6 22.8 23.9 25.1 26.3 27.5 28.6 29.8 31.0 32.2 33.4 34.6 35.8 37.0 38.2 39.5 40.7 41.913 14.8 16.0 17.1 18.3 19.5 20.6 21.8 23.0 24.2 25.4 26.6 27.8 29.0 30.2 31.4 32.6 33.8 35.0 36.2 37.5 38.7 39.9 41.2 42.4 43.614 16.1 17.3 18.5 19.7 20.9 22.1 23.3 24.5 25.7 26.9 28.1 29.3 30.5 31.7 33.0 34.2 35.4 36.7 37.9 39.1 40.4 41.6 42.9 44.2 45.415 17.5 18.7 19.9 21.1 22.3 23.5 24.7 25.9 27.2 28.4 29.6 30.9 32.1 33.3 34.6 35.8 37.1 38.4 39.6 40.9 42.2 43.4 44.7 46.0 47.316 18.9 20.1 21.3 22.6 23.8 25.0 26.2 27.5 28.7 30.0 31.2 32.5 33.7 35.0 36.3 37.5 38.8 40.1 41.4 42.7 44.0 45.3 46.6 47.9 49.217 20.3 21.6 22.8 24.1 25.3 26.6 27.8 29.1 30.3 31.6 32.9 34.1 35.4 36.7 38.0 39.3 40.6 41.9 43.2 44.5 45.9 47.2 48.5 49.8 51.218 21.8 23.1 24.3 25.6 26.9 28.1 29.4 30.7 32.0 33.3 34.6 35.9 37.2 38.5 39.8 41.1 42.4 43.8 45.1 46.5 47.8 49.2 50.5 51.9 53.219 23.3 24.6 25.9 27.2 28.5 29.8 31.1 32.4 33.7 35.0 36.3 37.6 39.0 40.3 41.6 43.0 44.3 45.7 47.1 48.4 49.8 51.2 52.6 54.0 55.420 24.9 26.2 27.5 28.8 30.1 31.5 32.8 34.1 35.4 36.8 38.1 39.5 40.8 42.2 43.6 44.9 46.3 47.7 49.1 50.5 51.9 53.3 54.7 56.1 57.621 26.5 27.9 29.2 30.5 31.8 33.2 34.5 35.9 37.3 38.6 40.0 41.4 42.8 44.1 45.5 46.9 48.4 49.8 51.2 52.6 54.1 55.5 56.9 58.4 59.922 28.2 29.5 30.9 32.3 33.6 35.0 36.4 37.7 39.1 40.5 41.9 43.3 44.8 46.2 47.6 49.0 50.5 51.9 53.4 54.8 56.3 57.8 59.3 60.8 62.323 29.9 31.3 32.7 34.1 35.5 36.8 38.3 39.7 41.1 42.5 43.9 45.4 46.8 48.3 49.7 51.2 52.7 54.2 55.6 57.1 58.6 60.2 61.7 63.2 64.724 31.7 33.1 34.5 35.9 37.3 38.8 40.2 41.7 43.1 44.6 46.0 47.5 49.0 50.5 52.0 53.5 55.0 56.5 58.0 59.5 61.1 62.6 64.2 65.8 67.325 33.6 35.0 36.4 37.9 39.3 40.8 42.2 43.7 45.2 46.7 48.2 49.7 51.2 52.7 54.3 55.8 57.3 58.9 60.5 62.0 63.6 65.2 66.8 68.4 70.026 35.5 36.9 38.4 39.9 41.4 42.8 44.3 45.9 47.4 48.9 50.4 52.0 53.5 55.1 56.7 58.2 59.8 61.4 63.0 64.7 66.3 67.9 69.6 71.2 72.927 37.4 38.9 40.4 42.0 43.5 45.0 46.5 48.1 49.6 51.2 52.8 54.4 56.0 57.6 59.2 60.8 62.4 64.1 65.7 67.4 69.1 70.8 72.5 74.2 75.928 39.5 41.0 42.6 44.1 45.7 47.3 48.8 50.4 52.0 53.6 55.2 56.9 58.5 60.2 61.8 63.5 65.2 66.9 68.6 70.3 72.0 73.7 75.5 77.3 79.029 41.7 43.2 44.8 46.4 48.0 49.6 51.2 52.8 54.5 56.1 57.8 59.5 61.2 62.9 64.6 66.3 68.0 69.8 71.5 73.3 75.1 76.9 78.7 80.5 82.430 43.9 45.5 47.1 48.7 50.4 52.0 53.7 55.4 57.1 58.8 60.5 62.2 64.0 65.7 67.5 69.3 71.0 72.9 74.7 76.5 78.3 80.2 82.1 84.0 85.931 46.2 47.9 49.5 51.2 52.9 54.6 56.3 58.1 59.8 61.6 63.3 65.1 66.9 68.7 70.5 72.4 74.2 76.1 78.0 79.9 81.8 83.7 85.7 87.6 89.632 48.7 50.4 52.1 53.8 55.6 57.3 59.1 60.9 62.7 64.5 66.3 68.2 70.0 71.9 73.8 75.7 77.6 79.5 81.5 83.5 85.4 87.5 89.5 91.5 93.633 51.2 53.0 54.8 56.5 58.3 60.2 62.0 63.8 65.7 67.6 69.5 71.4 73.3 75.2 77.2 79.2 81.2 83.2 85.2 87.3 89.3 91.4 93.6 95.7 97.834 53.9 55.7 57.6 59.4 61.3 63.1 65.0 67.0 68.9 70.8 72.8 74.8 76.8 78.8 80.8 82.9 85.0 87.1 89.2 91.4 93.5 95.7 97.9 100.2 102.435 56.8 58.6 60.5 62.4 64.4 66.3 68.3 70.3 72.3 74.3 76.3 78.4 80.5 82.6 84.7 86.9 89.1 91.3 93.5 95.7 98.0 100.3 102.6 105.0 107.336 59.8 61.7 63.7 65.7 67.7 69.7 71.7 73.8 75.9 78.0 80.1 82.3 84.5 86.7 88.9 91.2 93.5 95.8 98.1 100.5 102.9 105.3 107.7 110.2 112.737 62.9 65.0 67.0 69.1 71.2 73.3 75.4 77.6 79.8 82.0 84.2 86.5 88.8 91.1 93.4 95.8 98.2 100.6 103.1 105.6 108.1 110.7 113.3 115.9 118.638 66.3 68.4 70.6 72.7 74.9 77.1 79.4 81.6 83.9 86.2 88.6 91.0 93.4 95.8 98.3 100.8 103.4 105.9 108.6 111.2 113.9 116.6 119.4 122.2 125.039 70.0 72.2 74.4 76.7 78.9 81.3 83.6 86.0 88.4 90.9 93.4 95.9 98.4 101.0 103.6 106.3 109.0 111.8 114.6 117.4 120.3 123.2 126.1 129.2 132.240 73.8 76.2 78.5 80.9 83.3 85.7 88.2 90.8 93.3 95.9 98.5 101.2 103.9 106.7 109.5 112.4 115.3 118.2 121.2 124.3 127.4 130.5 133.7 137.0 140.341 78.0 80.5 83.0 85.5 88.0 90.6 93.3 95.9 98.7 101.4 104.3 107.1 110.0 113.0 116.0 119.1 122.2 125.4 128.7 132.0 135.4 138.8 142.3 145.9 149.542 82.6 85.2 87.8 90.5 93.2 96.0 98.8 101.7 104.6 107.6 110.6 113.7 116.9 120.1 123.4 126.7 130.1 133.6 137.2 140.8 144.5 148.3 152.2 156.1 160.243 87.6 90.4 93.2 96.0 99.0 101.9 105.0 108.1 111.2 114.5 117.8 121.1 124.6 128.1 131.7 135.4 139.1 143.0 147.0 151.0 155.2 159.4 163.8 168.2 172.844 93.1 96.1 99.1 102.2 105.4 108.6 111.9 115.3 118.7 122.3 125.9 129.6 133.4 137.4 141.4 145.5 149.7 154.1 158.5 163.1 167.9 172.7 177.7 182.9 188.245 99.3 102.5 105.8 109.2 112.6 116.2 119.8 123.6 127.4 131.4 135.4 139.6 143.9 148.3 152.9 157.6 162.4 167.4 172.6 178.0 183.5 189.2 195.1 201.2 207.546 106.3 109.8 113.4 117.2 121.0 125.0 129.1 133.3 137.6 142.1 146.7 151.5 156.5 161.6 167.0 172.5 178.2 184.2 190.4 196.8 203.5 210.5 217.8 225.4 233.347 114.3 118.3 122.4 126.6 130.9 135.4 140.1 145.0 150.0 155.3 160.7 166.4 172.3 178.5 185.0 191.8 198.9 206.4 214.2 222.4 231.0 240.0 249.5 259.5 270.048 123.9 128.4 133.1 137.9 143.0 148.3 153.9 159.7 165.8 172.2 178.9 186.0 193.5 201.4 209.8 218.7 228.2 238.2 248.9 260.3 272.3 285.1 298.7 313.0 328.249 135.5 140.8 146.4 152.3 158.5 165.0 172.0 179.3 187.2 195.6 204.6 214.3 224.7 235.9 248.1 261.3 275.5 290.9 307.6 325.5 344.8 365.4 387.3 410.6 435.2

Quanti-Tray e Defined Substrate Technology sono marchi di proprietà di, e/o registrati da, IDEXX Laboratories, Inc. e protetti negli Stati Uniti e/o in altri paesi. Protetto dai brevetti degli U.S.A. nn. 4,925,789; 5,429,933; 5,518,892. Altre domande di brevetto pendenti.
IDEXX Quanti-Tray/2000 Tabella MPN

# Pozzetti 

Grande 

Positivi

# Pozzetti Piccoli Positivi

25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48

0 25.3 26.4 27.4 28.4 29.5 30.5 31.5 32.6 33.6 34.7 35.7 36.8 37.8 38.9 40.0 41.0 42.1 43.1 44.2 45.3 46.3 47.4 48.5 49.51 26.6 27.7 28.7 29.8 30.8 31.9 32.9 34.0 35.0 36.1 37.2 38.2 39.3 40.4 41.4 42.5 43.6 44.7 45.7 46.8 47.9 49.0 50.1 51.22 27.9 29.0 30.0 31.1 32.2 33.2 34.3 35.4 36.5 37.5 38.6 39.7 40.8 41.9 43.0 44.0 45.1 46.2 47.3 48.4 49.5 50.6 51.7 52.83 29.3 30.4 31.4 32.5 33.6 34.7 35.8 36.8 37.9 39.0 40.1 41.2 42.3 43.4 44.5 45.6 46.7 47.8 48.9 50.0 51.2 52.3 53.4 54.54 30.7 31.8 32.8 33.9 35.0 36.1 37.2 38.3 39.4 40.5 41.6 42.8 43.9 45.0 46.1 47.2 48.3 49.5 50.6 51.7 52.9 54.0 55.1 56.35 32.1 33.2 34.3 35.4 36.5 37.6 38.7 39.9 41.0 42.1 43.2 44.4 45.5 46.6 47.7 48.9 50.0 51.2 52.3 53.5 54.6 55.8 56.9 58.16 33.5 34.7 35.8 36.9 38.0 39.2 40.3 41.4 42.6 43.7 44.8 46.0 47.1 48.3 49.4 50.6 51.7 52.9 54.1 55.2 56.4 57.6 58.7 59.97 35.0 36.2 37.3 38.4 39.6 40.7 41.9 43.0 44.2 45.3 46.5 47.7 48.8 50.0 51.2 52.3 53.5 54.7 55.9 57.1 58.3 59.4 60.6 61.88 36.6 37.7 38.9 40.0 41.2 42.3 43.5 44.7 45.9 47.0 48.2 49.4 50.6 51.8 53.0 54.1 55.3 56.5 57.7 59.0 60.2 61.4 62.6 63.89 38.1 39.3 40.5 41.6 42.8 44.0 45.2 46.4 47.6 48.8 50.0 51.2 52.4 53.6 54.8 56.0 57.2 58.4 59.7 60.9 62.1 63.4 64.6 65.8

10 39.7 40.9 42.1 43.3 44.5 45.7 46.9 48.1 49.3 50.6 51.8 53.0 54.2 55.5 56.7 57.9 59.2 60.4 61.7 62.9 64.2 65.4 66.7 67.9

11 41.4 42.6 43.8 45.0 46.3 47.5 48.7 49.9 51.2 52.4 53.7 54.9 56.1 57.4 58.6 59.9 61.2 62.4 63.7 65.0 66.3 67.5 68.8 70.1

12 43.1 44.3 45.6 46.8 48.1 49.3 50.6 51.8 53.1 54.3 55.6 56.8 58.1 59.4 60.7 62.0 63.2 64.5 65.8 67.1 68.4 69.7 71.0 72.413 44.9 46.1 47.4 48.6 49.9 51.2 52.5 53.7 55.0 56.3 57.6 58.9 60.2 61.5 62.8 64.1 65.4 66.7 68.0 69.3 70.7 72.0 73.3 74.714 46.7 48.0 49.3 50.5 51.8 53.1 54.4 55.7 57.0 58.3 59.6 60.9 62.3 63.6 64.9 66.3 67.6 68.9 70.3 71.6 73.0 74.4 75.7 77.115 48.6 49.9 51.2 52.5 53.8 55.1 56.4 57.8 59.1 60.4 61.8 63.1 64.5 65.8 67.2 68.5 69.9 71.3 72.6 74.0 75.4 76.8 78.2 79.616 50.5 51.8 53.2 54.5 55.8 57.2 58.5 59.9 61.2 62.6 64.0 65.3 66.7 68.1 69.5 70.9 72.3 73.7 75.1 76.5 77.9 79.3 80.8 82.217 52.5 53.9 55.2 56.6 58.0 59.3 60.7 62.1 63.5 64.9 66.3 67.7 69.1 70.5 71.9 73.3 74.8 76.2 77.6 79.1 80.5 82.0 83.5 84.918 54.6 56.0 57.4 58.8 60.2 61.6 63.0 64.4 65.8 67.2 68.6 70.1 71.5 73.0 74.4 75.9 77.3 78.8 80.3 81.8 83.3 84.8 86.3 87.819 56.8 58.2 59.6 61.0 62.4 63.9 65.3 66.8 68.2 69.7 71.1 72.6 74.1 75.5 77.0 78.5 80.0 81.5 83.1 84.6 86.1 87.6 89.2 90.720 59.0 60.4 61.9 63.3 64.8 66.3 67.7 69.2 70.7 72.2 73.7 75.2 76.7 78.2 79.8 81.3 82.8 84.4 85.9 87.5 89.1 90.7 92.2 93.821 61.3 62.8 64.3 65.8 67.3 68.8 70.3 71.8 73.3 74.9 76.4 77.9 79.5 81.1 82.6 84.2 85.8 87.4 89.0 90.6 92.2 93.8 95.4 97.122 63.8 65.3 66.8 68.3 69.8 71.4 72.9 74.5 76.1 77.6 79.2 80.8 82.4 84.0 85.6 87.2 88.9 90.5 92.1 93.8 95.5 97.1 98.8 100.523 66.3 67.8 69.4 71.0 72.5 74.1 75.7 77.3 78.9 80.5 82.2 83.8 85.4 87.1 88.7 90.4 92.1 93.8 95.5 97.2 98.9 100.6 102.4 104.124 68.9 70.5 72.1 73.7 75.3 77.0 78.6 80.3 81.9 83.6 85.2 86.9 88.6 90.3 92.0 93.8 95.5 97.2 99.0 100.7 102.5 104.3 106.1 107.925 71.7 73.3 75.0 76.6 78.3 80.0 81.7 83.3 85.1 86.8 88.5 90.2 92.0 93.7 95.5 97.3 99.1 100.9 102.7 104.5 106.3 108.2 110.0 111.926 74.6 76.3 78.0 79.7 81.4 83.1 84.8 86.6 88.4 90.1 91.9 93.7 95.5 97.3 99.2 101.0 102.9 104.7 106.6 108.5 110.4 112.3 114.2 116.227 77.6 79.4 81.1 82.9 84.6 86.4 88.2 90.0 91.9 93.7 95.5 97.4 99.3 101.2 103.1 105.0 106.9 108.8 110.8 112.7 114.7 116.7 118.7 120.728 80.8 82.6 84.4 86.3 88.1 89.9 91.8 93.7 95.6 97.5 99.4 101.3 103.3 105.2 107.2 109.2 111.2 113.2 115.2 117.3 119.3 121.4 123.5 125.629 84.2 86.1 87.9 89.8 91.7 93.7 95.6 97.5 99.5 101.5 103.5 105.5 107.5 109.5 111.6 113.7 115.7 117.8 120.0 122.1 124.2 126.4 128.6 130.830 87.8 89.7 91.7 93.6 95.6 97.6 99.6 101.6 103.7 105.7 107.8 109.9 112.0 114.2 116.3 118.5 120.6 122.8 125.1 127.3 129.5 131.8 134.1 136.431 91.6 93.6 95.6 97.7 99.7 101.8 103.9 106.0 108.2 110.3 112.5 114.7 116.9 119.1 121.4 123.6 125.9 128.2 130.5 132.9 135.3 137.7 140.1 142.532 95.7 97.8 99.9 102.0 104.2 106.3 108.5 110.7 113.0 115.2 117.5 119.8 122.1 124.5 126.8 129.2 131.6 134.0 136.5 139.0 141.5 144.0 146.6 149.133 100.0 102.2 104.4 106.6 108.9 111.2 113.5 115.8 118.2 120.5 122.9 125.4 127.8 130.3 132.8 135.3 137.8 140.4 143.0 145.6 148.3 150.9 153.7 156.434 104.7 107.0 109.3 111.7 114.0 116.4 118.9 121.3 123.8 126.3 128.8 131.4 134.0 136.6 139.2 141.9 144.6 147.4 150.1 152.9 155.7 158.6 161.5 164.435 109.7 112.2 114.6 117.1 119.6 122.2 124.7 127.3 129.9 132.6 135.3 138.0 140.8 143.6 146.4 149.2 152.1 155.0 158.0 161.0 164.0 167.1 170.2 173.336 115.2 117.8 120.4 123.0 125.7 128.4 131.1 133.9 136.7 139.5 142.4 145.3 148.3 151.3 154.3 157.3 160.5 163.6 166.8 170.0 173.3 176.6 179.9 183.337 121.3 124.0 126.8 129.6 132.4 135.3 138.2 141.2 144.2 147.3 150.3 153.5 156.7 159.9 163.1 166.5 169.8 173.2 176.7 180.2 183.7 187.3 191.0 194.738 127.9 130.8 133.8 136.8 139.9 143.0 146.2 149.4 152.6 155.9 159.2 162.6 166.1 169.6 173.2 176.8 180.4 184.2 188.0 191.8 195.7 199.7 203.7 207.739 135.3 138.5 141.7 145.0 148.3 151.7 155.1 158.6 162.1 165.7 169.4 173.1 176.9 180.7 184.7 188.7 192.7 196.8 201.0 205.3 209.6 214.0 218.5 223.040 143.7 147.1 150.6 154.2 157.8 161.5 165.3 169.1 173.0 177.0 181.1 185.2 189.4 193.7 198.1 202.5 207.1 211.7 216.4 221.1 226.0 231.0 236.0 241.141 153.2 157.0 160.9 164.8 168.9 173.0 177.2 181.5 185.8 190.3 194.8 199.5 204.2 209.1 214.0 219.1 224.2 229.4 234.8 240.2 245.8 251.5 257.2 263.142 164.3 168.6 172.9 177.3 181.9 186.5 191.3 196.1 201.1 206.2 211.4 216.7 222.2 227.7 233.4 239.2 245.2 251.3 257.5 263.8 270.3 276.9 283.6 290.543 177.5 182.3 187.3 192.4 197.6 202.9 208.4 214.0 219.8 225.8 231.8 238.1 244.5 251.0 257.7 264.6 271.7 278.9 286.3 293.8 301.5 309.4 317.4 325.744 193.6 199.3 205.1 211.0 217.2 223.5 230.0 236.7 243.6 250.8 258.1 265.6 273.3 281.2 289.4 297.8 306.3 315.1 324.1 333.3 342.8 352.4 362.3 372.445 214.1 220.9 227.9 235.2 242.7 250.4 258.4 266.7 275.3 284.1 293.3 302.6 312.3 322.3 332.5 343.0 353.8 364.9 376.2 387.9 399.8 412.0 424.5 437.446 241.5 250.0 258.9 268.2 277.8 287.8 298.1 308.8 319.9 331.4 343.3 355.5 368.1 381.1 394.5 408.3 422.5 437.1 452.0 467.4 483.3 499.6 516.3 533.547 280.9 292.4 304.4 316.9 330.0 343.6 357.8 372.5 387.7 403.4 419.8 436.6 454.1 472.1 490.7 509.9 529.8 550.4 571.7 593.8 616.7 640.5 665.3 691.048 344.1 360.9 378.4 396.8 416.0 436.0 456.9 478.6 501.2 524.7 549.3 574.8 601.5 629.4 658.6 689.3 721.5 755.6 791.5 829.7 870.4 913.9 960.6 1011.249 461.1 488.4 517.2 547.5 579.4 613.1 648.8 686.7 727.0 770.1 816.4 866.4 920.8 980.4 1046.2 1119.9 1203.3 1299.7 1413.6 1553.1 1732.9 1986.3 2419.6 >2419.6
IDEXX Quanti-Tray/2000

Versíon Español

Quanti-Tray y Defined Substrate Technology son marcas o marcas registradas de IDEXX Laboratories, Inc. en los 

Estados Unidos y/o otros paises. Patente norteamericanas numero 5,518,892; 5,620,895; and 5,753,456. Otras patentes 

pendientes de concesión.

Introducción

Los dispositivos Quanti-Tray/2000 de IDEXX están diseñados para producir recuentos bacterianos cuantificados de muestras de 100 mL, 

al ser utilizadas con productos de reactivos de la Tecnología del Sustrato Definido (Defined Substrate Technology) de IDEXX. Agregue 

la mezcla de reactivo y muestra a un dispositivo Quanti-Tray/2000, séllelo en el Quanti-Tray Sealer (Sellador) e incúbela según las 

instrucciones del reactivo. Luego cuente el número de celdas positivas y utilice la tabla de NMP adjunta para determinar el Número Más 

Probable (NMP).

Contenido

Este paquete contiene dispositivos Quanti-Tray/2000 estériles.

Soporte técnico de IDEXX

00800-4339-9111

www.idexx.es/agua

Instrucciones para el usuario

1. Sostenga en una mano el dispositivo 

Quanti-Tray en posición vertical, con 

el lado de las celdas orientado hacia 

la palma.

2. Apriete la parte superior de el 

dispositivo Quanti-Tray de modo de 

doblarla hacia la palma.

3. Abra el dispositivo Quanti-Tray 

desprendiendo la lengüeta metálica del 

lado que contiene las celdas. Evite tocar 

el interior del metal o del dispositivo.

4. Vierta la mezcla de reactivo y la 

muestra directamente dentro de el 

dispositivo Quanti-Tray, evitando tocar 

la lengüeta metálica. Golpear los 

pequeños pocillos 2 o 3 veces para 

eliminar posibles burbujas de aire. 

Deja reposar la espuma.

5. Coloque el dispositivo Quanti-Tray lleno 

de muestra sobre el portadispositivo 

de goma del sellador Quanti-Tray, 

orientando el lado de las celdas 

(plásticas) del dispositivo hacia 

abajo de manera que quepa en el 

portadispositivo.

6. Selle el dispositivo según las 

instrucciones del sellador.

7. Incube de acuerdo con las 

instrucciones del reactivo.

8. Cuente las celdas positivas. Para 

determinar el número más probable, 

recurra a la tabla NMP al dorso de 

esta hoja de instrucciones.

9. Verter el medio de cultivo conforme a 

Buenas Prácticas del Laboratorio.

IDEXX Laboratories, Inc.

One IDEXX Drive

Westbrook, Maine 04092 USA
IDEXX Quanti-Tray/2000 Tabla, número más probable

# Pocillos 

Grandes 

Positivos

# Pocillos pequeños positivos

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

0 <1 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.1 15.1 16.1 17.1 18.1 19.1 20.2 21.2 22.2 23.3 24.31 1.0 2.0 3.0 4.0 5.0 6.0 7.1 8.1 9.1 10.1 11.1 12.1 13.2 14.2 15.2 16.2 17.3 18.3 19.3 20.4 21.4 22.4 23.5 24.5 25.62 2.0 3.0 4.1 5.1 6.1 7.1 8.1 9.2 10.2 11.2 12.2 13.3 14.3 15.4 16.4 17.4 18.5 19.5 20.6 21.6 22.7 23.7 24.8 25.8 26.93 3.1 4.1 5.1 6.1 7.2 8.2 9.2 10.3 11.3 12.4 13.4 14.5 15.5 16.5 17.6 18.6 19.7 20.8 21.8 22.9 23.9 25.0 26.1 27.1 28.24 4.1 5.2 6.2 7.2 8.3 9.3 10.4 11.4 12.5 13.5 14.6 15.6 16.7 17.8 18.8 19.9 21.0 22.0 23.1 24.2 25.3 26.3 27.4 28.5 29.65 5.2 6.3 7.3 8.4 9.4 10.5 11.5 12.6 13.7 14.7 15.8 16.9 17.9 19.0 20.1 21.2 22.2 23.3 24.4 25.5 26.6 27.7 28.8 29.9 31.06 6.3 7.4 8.4 9.5 10.6 11.6 12.7 13.8 14.9 16.0 17.0 18.1 19.2 20.3 21.4 22.5 23.6 24.7 25.8 26.9 28.0 29.1 30.2 31.3 32.47 7.5 8.5 9.6 10.7 11.8 12.8 13.9 15.0 16.1 17.2 18.3 19.4 20.5 21.6 22.7 23.8 24.9 26.0 27.1 28.3 29.4 30.5 31.6 32.8 33.98 8.6 9.7 10.8 11.9 13.0 14.1 15.2 16.3 17.4 18.5 19.6 20.7 21.8 22.9 24.1 25.2 26.3 27.4 28.6 29.7 30.8 32.0 33.1 34.3 35.49 9.8 10.9 12.0 13.1 14.2 15.3 16.4 17.6 18.7 19.8 20.9 22.0 23.2 24.3 25.4 26.6 27.7 28.9 30.0 31.2 32.3 33.5 34.6 35.8 37.0

10 11.0 12.1 13.2 14.4 15.5 16.6 17.7 18.9 20.0 21.1 22.3 23.4 24.6 25.7 26.9 28.0 29.2 30.3 31.5 32.7 33.8 35.0 36.2 37.4 38.6

11 12.2 13.4 14.5 15.6 16.8 17.9 19.1 20.2 21.4 22.5 23.7 24.8 26.0 27.2 28.3 29.5 30.7 31.9 33.0 34.2 35.4 36.6 37.8 39.0 40.2

12 13.5 14.6 15.8 16.9 18.1 19.3 20.4 21.6 22.8 23.9 25.1 26.3 27.5 28.6 29.8 31.0 32.2 33.4 34.6 35.8 37.0 38.2 39.5 40.7 41.913 14.8 16.0 17.1 18.3 19.5 20.6 21.8 23.0 24.2 25.4 26.6 27.8 29.0 30.2 31.4 32.6 33.8 35.0 36.2 37.5 38.7 39.9 41.2 42.4 43.614 16.1 17.3 18.5 19.7 20.9 22.1 23.3 24.5 25.7 26.9 28.1 29.3 30.5 31.7 33.0 34.2 35.4 36.7 37.9 39.1 40.4 41.6 42.9 44.2 45.415 17.5 18.7 19.9 21.1 22.3 23.5 24.7 25.9 27.2 28.4 29.6 30.9 32.1 33.3 34.6 35.8 37.1 38.4 39.6 40.9 42.2 43.4 44.7 46.0 47.316 18.9 20.1 21.3 22.6 23.8 25.0 26.2 27.5 28.7 30.0 31.2 32.5 33.7 35.0 36.3 37.5 38.8 40.1 41.4 42.7 44.0 45.3 46.6 47.9 49.217 20.3 21.6 22.8 24.1 25.3 26.6 27.8 29.1 30.3 31.6 32.9 34.1 35.4 36.7 38.0 39.3 40.6 41.9 43.2 44.5 45.9 47.2 48.5 49.8 51.218 21.8 23.1 24.3 25.6 26.9 28.1 29.4 30.7 32.0 33.3 34.6 35.9 37.2 38.5 39.8 41.1 42.4 43.8 45.1 46.5 47.8 49.2 50.5 51.9 53.219 23.3 24.6 25.9 27.2 28.5 29.8 31.1 32.4 33.7 35.0 36.3 37.6 39.0 40.3 41.6 43.0 44.3 45.7 47.1 48.4 49.8 51.2 52.6 54.0 55.420 24.9 26.2 27.5 28.8 30.1 31.5 32.8 34.1 35.4 36.8 38.1 39.5 40.8 42.2 43.6 44.9 46.3 47.7 49.1 50.5 51.9 53.3 54.7 56.1 57.621 26.5 27.9 29.2 30.5 31.8 33.2 34.5 35.9 37.3 38.6 40.0 41.4 42.8 44.1 45.5 46.9 48.4 49.8 51.2 52.6 54.1 55.5 56.9 58.4 59.922 28.2 29.5 30.9 32.3 33.6 35.0 36.4 37.7 39.1 40.5 41.9 43.3 44.8 46.2 47.6 49.0 50.5 51.9 53.4 54.8 56.3 57.8 59.3 60.8 62.323 29.9 31.3 32.7 34.1 35.5 36.8 38.3 39.7 41.1 42.5 43.9 45.4 46.8 48.3 49.7 51.2 52.7 54.2 55.6 57.1 58.6 60.2 61.7 63.2 64.724 31.7 33.1 34.5 35.9 37.3 38.8 40.2 41.7 43.1 44.6 46.0 47.5 49.0 50.5 52.0 53.5 55.0 56.5 58.0 59.5 61.1 62.6 64.2 65.8 67.325 33.6 35.0 36.4 37.9 39.3 40.8 42.2 43.7 45.2 46.7 48.2 49.7 51.2 52.7 54.3 55.8 57.3 58.9 60.5 62.0 63.6 65.2 66.8 68.4 70.026 35.5 36.9 38.4 39.9 41.4 42.8 44.3 45.9 47.4 48.9 50.4 52.0 53.5 55.1 56.7 58.2 59.8 61.4 63.0 64.7 66.3 67.9 69.6 71.2 72.927 37.4 38.9 40.4 42.0 43.5 45.0 46.5 48.1 49.6 51.2 52.8 54.4 56.0 57.6 59.2 60.8 62.4 64.1 65.7 67.4 69.1 70.8 72.5 74.2 75.928 39.5 41.0 42.6 44.1 45.7 47.3 48.8 50.4 52.0 53.6 55.2 56.9 58.5 60.2 61.8 63.5 65.2 66.9 68.6 70.3 72.0 73.7 75.5 77.3 79.029 41.7 43.2 44.8 46.4 48.0 49.6 51.2 52.8 54.5 56.1 57.8 59.5 61.2 62.9 64.6 66.3 68.0 69.8 71.5 73.3 75.1 76.9 78.7 80.5 82.430 43.9 45.5 47.1 48.7 50.4 52.0 53.7 55.4 57.1 58.8 60.5 62.2 64.0 65.7 67.5 69.3 71.0 72.9 74.7 76.5 78.3 80.2 82.1 84.0 85.931 46.2 47.9 49.5 51.2 52.9 54.6 56.3 58.1 59.8 61.6 63.3 65.1 66.9 68.7 70.5 72.4 74.2 76.1 78.0 79.9 81.8 83.7 85.7 87.6 89.632 48.7 50.4 52.1 53.8 55.6 57.3 59.1 60.9 62.7 64.5 66.3 68.2 70.0 71.9 73.8 75.7 77.6 79.5 81.5 83.5 85.4 87.5 89.5 91.5 93.633 51.2 53.0 54.8 56.5 58.3 60.2 62.0 63.8 65.7 67.6 69.5 71.4 73.3 75.2 77.2 79.2 81.2 83.2 85.2 87.3 89.3 91.4 93.6 95.7 97.834 53.9 55.7 57.6 59.4 61.3 63.1 65.0 67.0 68.9 70.8 72.8 74.8 76.8 78.8 80.8 82.9 85.0 87.1 89.2 91.4 93.5 95.7 97.9 100.2 102.435 56.8 58.6 60.5 62.4 64.4 66.3 68.3 70.3 72.3 74.3 76.3 78.4 80.5 82.6 84.7 86.9 89.1 91.3 93.5 95.7 98.0 100.3 102.6 105.0 107.336 59.8 61.7 63.7 65.7 67.7 69.7 71.7 73.8 75.9 78.0 80.1 82.3 84.5 86.7 88.9 91.2 93.5 95.8 98.1 100.5 102.9 105.3 107.7 110.2 112.737 62.9 65.0 67.0 69.1 71.2 73.3 75.4 77.6 79.8 82.0 84.2 86.5 88.8 91.1 93.4 95.8 98.2 100.6 103.1 105.6 108.1 110.7 113.3 115.9 118.638 66.3 68.4 70.6 72.7 74.9 77.1 79.4 81.6 83.9 86.2 88.6 91.0 93.4 95.8 98.3 100.8 103.4 105.9 108.6 111.2 113.9 116.6 119.4 122.2 125.039 70.0 72.2 74.4 76.7 78.9 81.3 83.6 86.0 88.4 90.9 93.4 95.9 98.4 101.0 103.6 106.3 109.0 111.8 114.6 117.4 120.3 123.2 126.1 129.2 132.240 73.8 76.2 78.5 80.9 83.3 85.7 88.2 90.8 93.3 95.9 98.5 101.2 103.9 106.7 109.5 112.4 115.3 118.2 121.2 124.3 127.4 130.5 133.7 137.0 140.341 78.0 80.5 83.0 85.5 88.0 90.6 93.3 95.9 98.7 101.4 104.3 107.1 110.0 113.0 116.0 119.1 122.2 125.4 128.7 132.0 135.4 138.8 142.3 145.9 149.542 82.6 85.2 87.8 90.5 93.2 96.0 98.8 101.7 104.6 107.6 110.6 113.7 116.9 120.1 123.4 126.7 130.1 133.6 137.2 140.8 144.5 148.3 152.2 156.1 160.243 87.6 90.4 93.2 96.0 99.0 101.9 105.0 108.1 111.2 114.5 117.8 121.1 124.6 128.1 131.7 135.4 139.1 143.0 147.0 151.0 155.2 159.4 163.8 168.2 172.844 93.1 96.1 99.1 102.2 105.4 108.6 111.9 115.3 118.7 122.3 125.9 129.6 133.4 137.4 141.4 145.5 149.7 154.1 158.5 163.1 167.9 172.7 177.7 182.9 188.245 99.3 102.5 105.8 109.2 112.6 116.2 119.8 123.6 127.4 131.4 135.4 139.6 143.9 148.3 152.9 157.6 162.4 167.4 172.6 178.0 183.5 189.2 195.1 201.2 207.546 106.3 109.8 113.4 117.2 121.0 125.0 129.1 133.3 137.6 142.1 146.7 151.5 156.5 161.6 167.0 172.5 178.2 184.2 190.4 196.8 203.5 210.5 217.8 225.4 233.347 114.3 118.3 122.4 126.6 130.9 135.4 140.1 145.0 150.0 155.3 160.7 166.4 172.3 178.5 185.0 191.8 198.9 206.4 214.2 222.4 231.0 240.0 249.5 259.5 270.048 123.9 128.4 133.1 137.9 143.0 148.3 153.9 159.7 165.8 172.2 178.9 186.0 193.5 201.4 209.8 218.7 228.2 238.2 248.9 260.3 272.3 285.1 298.7 313.0 328.249 135.5 140.8 146.4 152.3 158.5 165.0 172.0 179.3 187.2 195.6 204.6 214.3 224.7 235.9 248.1 261.3 275.5 290.9 307.6 325.5 344.8 365.4 387.3 410.6 435.2

Quanti-Tray y Defined Substrate Technology son marcas o marcas registradas de IDEXX Laboratories, Inc. en los Estados Unidos y/o otros paises. Patente norteamericanas numero 4,925,789; 5,429,933; 5,518,892. Otras patentes pendientes de concesión.
IDEXX Quanti-Tray/2000 Tabla, número más probable

# Pocillos 

Grandes 

Positivos

# Pocillos pequeños positivos

25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48

0 25.3 26.4 27.4 28.4 29.5 30.5 31.5 32.6 33.6 34.7 35.7 36.8 37.8 38.9 40.0 41.0 42.1 43.1 44.2 45.3 46.3 47.4 48.5 49.51 26.6 27.7 28.7 29.8 30.8 31.9 32.9 34.0 35.0 36.1 37.2 38.2 39.3 40.4 41.4 42.5 43.6 44.7 45.7 46.8 47.9 49.0 50.1 51.22 27.9 29.0 30.0 31.1 32.2 33.2 34.3 35.4 36.5 37.5 38.6 39.7 40.8 41.9 43.0 44.0 45.1 46.2 47.3 48.4 49.5 50.6 51.7 52.83 29.3 30.4 31.4 32.5 33.6 34.7 35.8 36.8 37.9 39.0 40.1 41.2 42.3 43.4 44.5 45.6 46.7 47.8 48.9 50.0 51.2 52.3 53.4 54.54 30.7 31.8 32.8 33.9 35.0 36.1 37.2 38.3 39.4 40.5 41.6 42.8 43.9 45.0 46.1 47.2 48.3 49.5 50.6 51.7 52.9 54.0 55.1 56.35 32.1 33.2 34.3 35.4 36.5 37.6 38.7 39.9 41.0 42.1 43.2 44.4 45.5 46.6 47.7 48.9 50.0 51.2 52.3 53.5 54.6 55.8 56.9 58.16 33.5 34.7 35.8 36.9 38.0 39.2 40.3 41.4 42.6 43.7 44.8 46.0 47.1 48.3 49.4 50.6 51.7 52.9 54.1 55.2 56.4 57.6 58.7 59.97 35.0 36.2 37.3 38.4 39.6 40.7 41.9 43.0 44.2 45.3 46.5 47.7 48.8 50.0 51.2 52.3 53.5 54.7 55.9 57.1 58.3 59.4 60.6 61.88 36.6 37.7 38.9 40.0 41.2 42.3 43.5 44.7 45.9 47.0 48.2 49.4 50.6 51.8 53.0 54.1 55.3 56.5 57.7 59.0 60.2 61.4 62.6 63.89 38.1 39.3 40.5 41.6 42.8 44.0 45.2 46.4 47.6 48.8 50.0 51.2 52.4 53.6 54.8 56.0 57.2 58.4 59.7 60.9 62.1 63.4 64.6 65.8

10 39.7 40.9 42.1 43.3 44.5 45.7 46.9 48.1 49.3 50.6 51.8 53.0 54.2 55.5 56.7 57.9 59.2 60.4 61.7 62.9 64.2 65.4 66.7 67.9

11 41.4 42.6 43.8 45.0 46.3 47.5 48.7 49.9 51.2 52.4 53.7 54.9 56.1 57.4 58.6 59.9 61.2 62.4 63.7 65.0 66.3 67.5 68.8 70.1

12 43.1 44.3 45.6 46.8 48.1 49.3 50.6 51.8 53.1 54.3 55.6 56.8 58.1 59.4 60.7 62.0 63.2 64.5 65.8 67.1 68.4 69.7 71.0 72.413 44.9 46.1 47.4 48.6 49.9 51.2 52.5 53.7 55.0 56.3 57.6 58.9 60.2 61.5 62.8 64.1 65.4 66.7 68.0 69.3 70.7 72.0 73.3 74.714 46.7 48.0 49.3 50.5 51.8 53.1 54.4 55.7 57.0 58.3 59.6 60.9 62.3 63.6 64.9 66.3 67.6 68.9 70.3 71.6 73.0 74.4 75.7 77.115 48.6 49.9 51.2 52.5 53.8 55.1 56.4 57.8 59.1 60.4 61.8 63.1 64.5 65.8 67.2 68.5 69.9 71.3 72.6 74.0 75.4 76.8 78.2 79.616 50.5 51.8 53.2 54.5 55.8 57.2 58.5 59.9 61.2 62.6 64.0 65.3 66.7 68.1 69.5 70.9 72.3 73.7 75.1 76.5 77.9 79.3 80.8 82.217 52.5 53.9 55.2 56.6 58.0 59.3 60.7 62.1 63.5 64.9 66.3 67.7 69.1 70.5 71.9 73.3 74.8 76.2 77.6 79.1 80.5 82.0 83.5 84.918 54.6 56.0 57.4 58.8 60.2 61.6 63.0 64.4 65.8 67.2 68.6 70.1 71.5 73.0 74.4 75.9 77.3 78.8 80.3 81.8 83.3 84.8 86.3 87.819 56.8 58.2 59.6 61.0 62.4 63.9 65.3 66.8 68.2 69.7 71.1 72.6 74.1 75.5 77.0 78.5 80.0 81.5 83.1 84.6 86.1 87.6 89.2 90.720 59.0 60.4 61.9 63.3 64.8 66.3 67.7 69.2 70.7 72.2 73.7 75.2 76.7 78.2 79.8 81.3 82.8 84.4 85.9 87.5 89.1 90.7 92.2 93.821 61.3 62.8 64.3 65.8 67.3 68.8 70.3 71.8 73.3 74.9 76.4 77.9 79.5 81.1 82.6 84.2 85.8 87.4 89.0 90.6 92.2 93.8 95.4 97.122 63.8 65.3 66.8 68.3 69.8 71.4 72.9 74.5 76.1 77.6 79.2 80.8 82.4 84.0 85.6 87.2 88.9 90.5 92.1 93.8 95.5 97.1 98.8 100.523 66.3 67.8 69.4 71.0 72.5 74.1 75.7 77.3 78.9 80.5 82.2 83.8 85.4 87.1 88.7 90.4 92.1 93.8 95.5 97.2 98.9 100.6 102.4 104.124 68.9 70.5 72.1 73.7 75.3 77.0 78.6 80.3 81.9 83.6 85.2 86.9 88.6 90.3 92.0 93.8 95.5 97.2 99.0 100.7 102.5 104.3 106.1 107.925 71.7 73.3 75.0 76.6 78.3 80.0 81.7 83.3 85.1 86.8 88.5 90.2 92.0 93.7 95.5 97.3 99.1 100.9 102.7 104.5 106.3 108.2 110.0 111.926 74.6 76.3 78.0 79.7 81.4 83.1 84.8 86.6 88.4 90.1 91.9 93.7 95.5 97.3 99.2 101.0 102.9 104.7 106.6 108.5 110.4 112.3 114.2 116.227 77.6 79.4 81.1 82.9 84.6 86.4 88.2 90.0 91.9 93.7 95.5 97.4 99.3 101.2 103.1 105.0 106.9 108.8 110.8 112.7 114.7 116.7 118.7 120.728 80.8 82.6 84.4 86.3 88.1 89.9 91.8 93.7 95.6 97.5 99.4 101.3 103.3 105.2 107.2 109.2 111.2 113.2 115.2 117.3 119.3 121.4 123.5 125.629 84.2 86.1 87.9 89.8 91.7 93.7 95.6 97.5 99.5 101.5 103.5 105.5 107.5 109.5 111.6 113.7 115.7 117.8 120.0 122.1 124.2 126.4 128.6 130.830 87.8 89.7 91.7 93.6 95.6 97.6 99.6 101.6 103.7 105.7 107.8 109.9 112.0 114.2 116.3 118.5 120.6 122.8 125.1 127.3 129.5 131.8 134.1 136.431 91.6 93.6 95.6 97.7 99.7 101.8 103.9 106.0 108.2 110.3 112.5 114.7 116.9 119.1 121.4 123.6 125.9 128.2 130.5 132.9 135.3 137.7 140.1 142.532 95.7 97.8 99.9 102.0 104.2 106.3 108.5 110.7 113.0 115.2 117.5 119.8 122.1 124.5 126.8 129.2 131.6 134.0 136.5 139.0 141.5 144.0 146.6 149.133 100.0 102.2 104.4 106.6 108.9 111.2 113.5 115.8 118.2 120.5 122.9 125.4 127.8 130.3 132.8 135.3 137.8 140.4 143.0 145.6 148.3 150.9 153.7 156.434 104.7 107.0 109.3 111.7 114.0 116.4 118.9 121.3 123.8 126.3 128.8 131.4 134.0 136.6 139.2 141.9 144.6 147.4 150.1 152.9 155.7 158.6 161.5 164.435 109.7 112.2 114.6 117.1 119.6 122.2 124.7 127.3 129.9 132.6 135.3 138.0 140.8 143.6 146.4 149.2 152.1 155.0 158.0 161.0 164.0 167.1 170.2 173.336 115.2 117.8 120.4 123.0 125.7 128.4 131.1 133.9 136.7 139.5 142.4 145.3 148.3 151.3 154.3 157.3 160.5 163.6 166.8 170.0 173.3 176.6 179.9 183.337 121.3 124.0 126.8 129.6 132.4 135.3 138.2 141.2 144.2 147.3 150.3 153.5 156.7 159.9 163.1 166.5 169.8 173.2 176.7 180.2 183.7 187.3 191.0 194.738 127.9 130.8 133.8 136.8 139.9 143.0 146.2 149.4 152.6 155.9 159.2 162.6 166.1 169.6 173.2 176.8 180.4 184.2 188.0 191.8 195.7 199.7 203.7 207.739 135.3 138.5 141.7 145.0 148.3 151.7 155.1 158.6 162.1 165.7 169.4 173.1 176.9 180.7 184.7 188.7 192.7 196.8 201.0 205.3 209.6 214.0 218.5 223.040 143.7 147.1 150.6 154.2 157.8 161.5 165.3 169.1 173.0 177.0 181.1 185.2 189.4 193.7 198.1 202.5 207.1 211.7 216.4 221.1 226.0 231.0 236.0 241.141 153.2 157.0 160.9 164.8 168.9 173.0 177.2 181.5 185.8 190.3 194.8 199.5 204.2 209.1 214.0 219.1 224.2 229.4 234.8 240.2 245.8 251.5 257.2 263.142 164.3 168.6 172.9 177.3 181.9 186.5 191.3 196.1 201.1 206.2 211.4 216.7 222.2 227.7 233.4 239.2 245.2 251.3 257.5 263.8 270.3 276.9 283.6 290.543 177.5 182.3 187.3 192.4 197.6 202.9 208.4 214.0 219.8 225.8 231.8 238.1 244.5 251.0 257.7 264.6 271.7 278.9 286.3 293.8 301.5 309.4 317.4 325.744 193.6 199.3 205.1 211.0 217.2 223.5 230.0 236.7 243.6 250.8 258.1 265.6 273.3 281.2 289.4 297.8 306.3 315.1 324.1 333.3 342.8 352.4 362.3 372.445 214.1 220.9 227.9 235.2 242.7 250.4 258.4 266.7 275.3 284.1 293.3 302.6 312.3 322.3 332.5 343.0 353.8 364.9 376.2 387.9 399.8 412.0 424.5 437.446 241.5 250.0 258.9 268.2 277.8 287.8 298.1 308.8 319.9 331.4 343.3 355.5 368.1 381.1 394.5 408.3 422.5 437.1 452.0 467.4 483.3 499.6 516.3 533.547 280.9 292.4 304.4 316.9 330.0 343.6 357.8 372.5 387.7 403.4 419.8 436.6 454.1 472.1 490.7 509.9 529.8 550.4 571.7 593.8 616.7 640.5 665.3 691.048 344.1 360.9 378.4 396.8 416.0 436.0 456.9 478.6 501.2 524.7 549.3 574.8 601.5 629.4 658.6 689.3 721.5 755.6 791.5 829.7 870.4 913.9 960.6 1011.249 461.1 488.4 517.2 547.5 579.4 613.1 648.8 686.7 727.0 770.1 816.4 866.4 920.8 980.4 1046.2 1119.9 1203.3 1299.7 1413.6 1553.1 1732.9 1986.3 2419.6 >2419.6
IDEXX Quanti-Tray/2000

Deutsche Version

Quanti-Tray und Defined Substrate Technology sind Schutzmarken oder eingetragene Schutzmarken von 

IDEXX Laboratories, Inc. in den Vereinigten Staaten und/oder in anderen Ländern. Geschützt durch U.S.-Patent 

Nummern 5,518,892; 5,620,895; and 5,753,456. Andere Patenten angemeldet.

Einführung

Quanti-Tray/2000 von IDEXX dienen zur quantitativen Bakterienzählung in 100-mL-Proben unter Verwendung von IDEXX Defined 

Substrate Technology-Reagenzien. Die Mischung aus Probe und Reagenz in einen Quanti-Tray/2000 geben, diesen in einem Quanti-TrayVersiegelungsgerät verschließen und laut Reagenz-Packungsbeilage inkubieren. Anschließend die Anzahl der positiven Vertiefungen 

zählen und mit der beigefügten MPN-Tabelle die “Wahrscheinlichste Anzahl” (MPN) bestimmen.

Inhalt

Diese Packung enthält sterile Quanti-Tray/2000.

IDEXX Technischer Dienst

00800-4339-9111

www.idexx.de/wasser

Gebrauchsanweisung

1. Quanti-Tray mit einer Hand fassen und 

senkrecht halten, wobei die Seite mit 

den Vertiefungen zur Handfläche zeigt.

2. Den oberen Teil des Quanti-Trays 

zusammendrücken, so daß sich der 

Träger zur Handfläche hin biegt.

3. Quanti-Tray öffnen, indem die 

Folienlasche von der Seite mit den 

Vertiefungen abgezogen wird. Die 

Innenseite der Folie oder des Trays 

nicht berühren.

4. Die Mischung aus Probe und 

Reagenz direkt in einen Quanti-Tray 

gießen. Dabei eine Berührung der 

Folienlasche vermeiden. Seitlich 

2–3 mal an die kleinen Vertiefungen 

klopfen, um eventuelle Luftblasen zu 

entfernen. Schaum einwirken lassen.

5. Den mit der Probe gefüllten QuantiTray auf die Gummi-Trägerunterlage 

des Quanti-Tray-Versiegelungsgeräts 

legen. Die Seite mit den Vertiefungen 

(Kunststoff) des Quanti-Trays muß 

dabei nach unten zeigen.

6. Das Verschließen erfolgt 

laut Anweisungen für das 

Versiegelungsgerät.

7. Entsprechend den 

Reagenzanweisungen inkubieren.

8. Die positiven Vertiefungen zählen 

und mit Hilfe der MPN-Tabelle auf der 

Rückseite dieser Packungsbeilage 

die Wahrscheinlichste Anzahl (MPN) 

ermitteln.

9. Entsorgen Sie die Quanti-Trays gemäß 

guter Laborpraxis.

IDEXX Laboratories, Inc.

One IDEXX Drive

Westbrook, Maine 04092 USA
IDEXX Quanti-Tray/2000 MPN-Tabelle

# Positiven 

Großen 

Vertiefungen

# Positiven Kleinen Vertiefungen

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

0 <1 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.1 15.1 16.1 17.1 18.1 19.1 20.2 21.2 22.2 23.3 24.31 1.0 2.0 3.0 4.0 5.0 6.0 7.1 8.1 9.1 10.1 11.1 12.1 13.2 14.2 15.2 16.2 17.3 18.3 19.3 20.4 21.4 22.4 23.5 24.5 25.62 2.0 3.0 4.1 5.1 6.1 7.1 8.1 9.2 10.2 11.2 12.2 13.3 14.3 15.4 16.4 17.4 18.5 19.5 20.6 21.6 22.7 23.7 24.8 25.8 26.93 3.1 4.1 5.1 6.1 7.2 8.2 9.2 10.3 11.3 12.4 13.4 14.5 15.5 16.5 17.6 18.6 19.7 20.8 21.8 22.9 23.9 25.0 26.1 27.1 28.24 4.1 5.2 6.2 7.2 8.3 9.3 10.4 11.4 12.5 13.5 14.6 15.6 16.7 17.8 18.8 19.9 21.0 22.0 23.1 24.2 25.3 26.3 27.4 28.5 29.65 5.2 6.3 7.3 8.4 9.4 10.5 11.5 12.6 13.7 14.7 15.8 16.9 17.9 19.0 20.1 21.2 22.2 23.3 24.4 25.5 26.6 27.7 28.8 29.9 31.06 6.3 7.4 8.4 9.5 10.6 11.6 12.7 13.8 14.9 16.0 17.0 18.1 19.2 20.3 21.4 22.5 23.6 24.7 25.8 26.9 28.0 29.1 30.2 31.3 32.47 7.5 8.5 9.6 10.7 11.8 12.8 13.9 15.0 16.1 17.2 18.3 19.4 20.5 21.6 22.7 23.8 24.9 26.0 27.1 28.3 29.4 30.5 31.6 32.8 33.98 8.6 9.7 10.8 11.9 13.0 14.1 15.2 16.3 17.4 18.5 19.6 20.7 21.8 22.9 24.1 25.2 26.3 27.4 28.6 29.7 30.8 32.0 33.1 34.3 35.49 9.8 10.9 12.0 13.1 14.2 15.3 16.4 17.6 18.7 19.8 20.9 22.0 23.2 24.3 25.4 26.6 27.7 28.9 30.0 31.2 32.3 33.5 34.6 35.8 37.0

10 11.0 12.1 13.2 14.4 15.5 16.6 17.7 18.9 20.0 21.1 22.3 23.4 24.6 25.7 26.9 28.0 29.2 30.3 31.5 32.7 33.8 35.0 36.2 37.4 38.6

11 12.2 13.4 14.5 15.6 16.8 17.9 19.1 20.2 21.4 22.5 23.7 24.8 26.0 27.2 28.3 29.5 30.7 31.9 33.0 34.2 35.4 36.6 37.8 39.0 40.2

12 13.5 14.6 15.8 16.9 18.1 19.3 20.4 21.6 22.8 23.9 25.1 26.3 27.5 28.6 29.8 31.0 32.2 33.4 34.6 35.8 37.0 38.2 39.5 40.7 41.913 14.8 16.0 17.1 18.3 19.5 20.6 21.8 23.0 24.2 25.4 26.6 27.8 29.0 30.2 31.4 32.6 33.8 35.0 36.2 37.5 38.7 39.9 41.2 42.4 43.614 16.1 17.3 18.5 19.7 20.9 22.1 23.3 24.5 25.7 26.9 28.1 29.3 30.5 31.7 33.0 34.2 35.4 36.7 37.9 39.1 40.4 41.6 42.9 44.2 45.415 17.5 18.7 19.9 21.1 22.3 23.5 24.7 25.9 27.2 28.4 29.6 30.9 32.1 33.3 34.6 35.8 37.1 38.4 39.6 40.9 42.2 43.4 44.7 46.0 47.316 18.9 20.1 21.3 22.6 23.8 25.0 26.2 27.5 28.7 30.0 31.2 32.5 33.7 35.0 36.3 37.5 38.8 40.1 41.4 42.7 44.0 45.3 46.6 47.9 49.217 20.3 21.6 22.8 24.1 25.3 26.6 27.8 29.1 30.3 31.6 32.9 34.1 35.4 36.7 38.0 39.3 40.6 41.9 43.2 44.5 45.9 47.2 48.5 49.8 51.218 21.8 23.1 24.3 25.6 26.9 28.1 29.4 30.7 32.0 33.3 34.6 35.9 37.2 38.5 39.8 41.1 42.4 43.8 45.1 46.5 47.8 49.2 50.5 51.9 53.219 23.3 24.6 25.9 27.2 28.5 29.8 31.1 32.4 33.7 35.0 36.3 37.6 39.0 40.3 41.6 43.0 44.3 45.7 47.1 48.4 49.8 51.2 52.6 54.0 55.420 24.9 26.2 27.5 28.8 30.1 31.5 32.8 34.1 35.4 36.8 38.1 39.5 40.8 42.2 43.6 44.9 46.3 47.7 49.1 50.5 51.9 53.3 54.7 56.1 57.621 26.5 27.9 29.2 30.5 31.8 33.2 34.5 35.9 37.3 38.6 40.0 41.4 42.8 44.1 45.5 46.9 48.4 49.8 51.2 52.6 54.1 55.5 56.9 58.4 59.922 28.2 29.5 30.9 32.3 33.6 35.0 36.4 37.7 39.1 40.5 41.9 43.3 44.8 46.2 47.6 49.0 50.5 51.9 53.4 54.8 56.3 57.8 59.3 60.8 62.323 29.9 31.3 32.7 34.1 35.5 36.8 38.3 39.7 41.1 42.5 43.9 45.4 46.8 48.3 49.7 51.2 52.7 54.2 55.6 57.1 58.6 60.2 61.7 63.2 64.724 31.7 33.1 34.5 35.9 37.3 38.8 40.2 41.7 43.1 44.6 46.0 47.5 49.0 50.5 52.0 53.5 55.0 56.5 58.0 59.5 61.1 62.6 64.2 65.8 67.325 33.6 35.0 36.4 37.9 39.3 40.8 42.2 43.7 45.2 46.7 48.2 49.7 51.2 52.7 54.3 55.8 57.3 58.9 60.5 62.0 63.6 65.2 66.8 68.4 70.026 35.5 36.9 38.4 39.9 41.4 42.8 44.3 45.9 47.4 48.9 50.4 52.0 53.5 55.1 56.7 58.2 59.8 61.4 63.0 64.7 66.3 67.9 69.6 71.2 72.927 37.4 38.9 40.4 42.0 43.5 45.0 46.5 48.1 49.6 51.2 52.8 54.4 56.0 57.6 59.2 60.8 62.4 64.1 65.7 67.4 69.1 70.8 72.5 74.2 75.928 39.5 41.0 42.6 44.1 45.7 47.3 48.8 50.4 52.0 53.6 55.2 56.9 58.5 60.2 61.8 63.5 65.2 66.9 68.6 70.3 72.0 73.7 75.5 77.3 79.029 41.7 43.2 44.8 46.4 48.0 49.6 51.2 52.8 54.5 56.1 57.8 59.5 61.2 62.9 64.6 66.3 68.0 69.8 71.5 73.3 75.1 76.9 78.7 80.5 82.430 43.9 45.5 47.1 48.7 50.4 52.0 53.7 55.4 57.1 58.8 60.5 62.2 64.0 65.7 67.5 69.3 71.0 72.9 74.7 76.5 78.3 80.2 82.1 84.0 85.931 46.2 47.9 49.5 51.2 52.9 54.6 56.3 58.1 59.8 61.6 63.3 65.1 66.9 68.7 70.5 72.4 74.2 76.1 78.0 79.9 81.8 83.7 85.7 87.6 89.632 48.7 50.4 52.1 53.8 55.6 57.3 59.1 60.9 62.7 64.5 66.3 68.2 70.0 71.9 73.8 75.7 77.6 79.5 81.5 83.5 85.4 87.5 89.5 91.5 93.633 51.2 53.0 54.8 56.5 58.3 60.2 62.0 63.8 65.7 67.6 69.5 71.4 73.3 75.2 77.2 79.2 81.2 83.2 85.2 87.3 89.3 91.4 93.6 95.7 97.834 53.9 55.7 57.6 59.4 61.3 63.1 65.0 67.0 68.9 70.8 72.8 74.8 76.8 78.8 80.8 82.9 85.0 87.1 89.2 91.4 93.5 95.7 97.9 100.2 102.435 56.8 58.6 60.5 62.4 64.4 66.3 68.3 70.3 72.3 74.3 76.3 78.4 80.5 82.6 84.7 86.9 89.1 91.3 93.5 95.7 98.0 100.3 102.6 105.0 107.336 59.8 61.7 63.7 65.7 67.7 69.7 71.7 73.8 75.9 78.0 80.1 82.3 84.5 86.7 88.9 91.2 93.5 95.8 98.1 100.5 102.9 105.3 107.7 110.2 112.737 62.9 65.0 67.0 69.1 71.2 73.3 75.4 77.6 79.8 82.0 84.2 86.5 88.8 91.1 93.4 95.8 98.2 100.6 103.1 105.6 108.1 110.7 113.3 115.9 118.638 66.3 68.4 70.6 72.7 74.9 77.1 79.4 81.6 83.9 86.2 88.6 91.0 93.4 95.8 98.3 100.8 103.4 105.9 108.6 111.2 113.9 116.6 119.4 122.2 125.039 70.0 72.2 74.4 76.7 78.9 81.3 83.6 86.0 88.4 90.9 93.4 95.9 98.4 101.0 103.6 106.3 109.0 111.8 114.6 117.4 120.3 123.2 126.1 129.2 132.240 73.8 76.2 78.5 80.9 83.3 85.7 88.2 90.8 93.3 95.9 98.5 101.2 103.9 106.7 109.5 112.4 115.3 118.2 121.2 124.3 127.4 130.5 133.7 137.0 140.341 78.0 80.5 83.0 85.5 88.0 90.6 93.3 95.9 98.7 101.4 104.3 107.1 110.0 113.0 116.0 119.1 122.2 125.4 128.7 132.0 135.4 138.8 142.3 145.9 149.542 82.6 85.2 87.8 90.5 93.2 96.0 98.8 101.7 104.6 107.6 110.6 113.7 116.9 120.1 123.4 126.7 130.1 133.6 137.2 140.8 144.5 148.3 152.2 156.1 160.243 87.6 90.4 93.2 96.0 99.0 101.9 105.0 108.1 111.2 114.5 117.8 121.1 124.6 128.1 131.7 135.4 139.1 143.0 147.0 151.0 155.2 159.4 163.8 168.2 172.844 93.1 96.1 99.1 102.2 105.4 108.6 111.9 115.3 118.7 122.3 125.9 129.6 133.4 137.4 141.4 145.5 149.7 154.1 158.5 163.1 167.9 172.7 177.7 182.9 188.245 99.3 102.5 105.8 109.2 112.6 116.2 119.8 123.6 127.4 131.4 135.4 139.6 143.9 148.3 152.9 157.6 162.4 167.4 172.6 178.0 183.5 189.2 195.1 201.2 207.546 106.3 109.8 113.4 117.2 121.0 125.0 129.1 133.3 137.6 142.1 146.7 151.5 156.5 161.6 167.0 172.5 178.2 184.2 190.4 196.8 203.5 210.5 217.8 225.4 233.347 114.3 118.3 122.4 126.6 130.9 135.4 140.1 145.0 150.0 155.3 160.7 166.4 172.3 178.5 185.0 191.8 198.9 206.4 214.2 222.4 231.0 240.0 249.5 259.5 270.048 123.9 128.4 133.1 137.9 143.0 148.3 153.9 159.7 165.8 172.2 178.9 186.0 193.5 201.4 209.8 218.7 228.2 238.2 248.9 260.3 272.3 285.1 298.7 313.0 328.249 135.5 140.8 146.4 152.3 158.5 165.0 172.0 179.3 187.2 195.6 204.6 214.3 224.7 235.9 248.1 261.3 275.5 290.9 307.6 325.5 344.8 365.4 387.3 410.6 435.2

Quanti-Tray und Defined Substrate Technology sind Schutzmarken oder eingetragene Schutzmarken von IDEXX Laboratories, Inc. in den Vereinigten Staaten und/oder in anderen Ländern. Geschützt durch U.S.-Patent Nummern 4,925,789; 5,429,933; 5,518,892. Andere Patenten angemeldet.
IDEXX Quanti-Tray/2000 MPN-Tabelle

# Positiven 

Großen 

Vertiefungen

# Positiven Kleinen Vertiefungen

25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48

0 25.3 26.4 27.4 28.4 29.5 30.5 31.5 32.6 33.6 34.7 35.7 36.8 37.8 38.9 40.0 41.0 42.1 43.1 44.2 45.3 46.3 47.4 48.5 49.51 26.6 27.7 28.7 29.8 30.8 31.9 32.9 34.0 35.0 36.1 37.2 38.2 39.3 40.4 41.4 42.5 43.6 44.7 45.7 46.8 47.9 49.0 50.1 51.22 27.9 29.0 30.0 31.1 32.2 33.2 34.3 35.4 36.5 37.5 38.6 39.7 40.8 41.9 43.0 44.0 45.1 46.2 47.3 48.4 49.5 50.6 51.7 52.83 29.3 30.4 31.4 32.5 33.6 34.7 35.8 36.8 37.9 39.0 40.1 41.2 42.3 43.4 44.5 45.6 46.7 47.8 48.9 50.0 51.2 52.3 53.4 54.54 30.7 31.8 32.8 33.9 35.0 36.1 37.2 38.3 39.4 40.5 41.6 42.8 43.9 45.0 46.1 47.2 48.3 49.5 50.6 51.7 52.9 54.0 55.1 56.35 32.1 33.2 34.3 35.4 36.5 37.6 38.7 39.9 41.0 42.1 43.2 44.4 45.5 46.6 47.7 48.9 50.0 51.2 52.3 53.5 54.6 55.8 56.9 58.16 33.5 34.7 35.8 36.9 38.0 39.2 40.3 41.4 42.6 43.7 44.8 46.0 47.1 48.3 49.4 50.6 51.7 52.9 54.1 55.2 56.4 57.6 58.7 59.97 35.0 36.2 37.3 38.4 39.6 40.7 41.9 43.0 44.2 45.3 46.5 47.7 48.8 50.0 51.2 52.3 53.5 54.7 55.9 57.1 58.3 59.4 60.6 61.88 36.6 37.7 38.9 40.0 41.2 42.3 43.5 44.7 45.9 47.0 48.2 49.4 50.6 51.8 53.0 54.1 55.3 56.5 57.7 59.0 60.2 61.4 62.6 63.89 38.1 39.3 40.5 41.6 42.8 44.0 45.2 46.4 47.6 48.8 50.0 51.2 52.4 53.6 54.8 56.0 57.2 58.4 59.7 60.9 62.1 63.4 64.6 65.8

10 39.7 40.9 42.1 43.3 44.5 45.7 46.9 48.1 49.3 50.6 51.8 53.0 54.2 55.5 56.7 57.9 59.2 60.4 61.7 62.9 64.2 65.4 66.7 67.9

11 41.4 42.6 43.8 45.0 46.3 47.5 48.7 49.9 51.2 52.4 53.7 54.9 56.1 57.4 58.6 59.9 61.2 62.4 63.7 65.0 66.3 67.5 68.8 70.1

12 43.1 44.3 45.6 46.8 48.1 49.3 50.6 51.8 53.1 54.3 55.6 56.8 58.1 59.4 60.7 62.0 63.2 64.5 65.8 67.1 68.4 69.7 71.0 72.413 44.9 46.1 47.4 48.6 49.9 51.2 52.5 53.7 55.0 56.3 57.6 58.9 60.2 61.5 62.8 64.1 65.4 66.7 68.0 69.3 70.7 72.0 73.3 74.714 46.7 48.0 49.3 50.5 51.8 53.1 54.4 55.7 57.0 58.3 59.6 60.9 62.3 63.6 64.9 66.3 67.6 68.9 70.3 71.6 73.0 74.4 75.7 77.115 48.6 49.9 51.2 52.5 53.8 55.1 56.4 57.8 59.1 60.4 61.8 63.1 64.5 65.8 67.2 68.5 69.9 71.3 72.6 74.0 75.4 76.8 78.2 79.616 50.5 51.8 53.2 54.5 55.8 57.2 58.5 59.9 61.2 62.6 64.0 65.3 66.7 68.1 69.5 70.9 72.3 73.7 75.1 76.5 77.9 79.3 80.8 82.217 52.5 53.9 55.2 56.6 58.0 59.3 60.7 62.1 63.5 64.9 66.3 67.7 69.1 70.5 71.9 73.3 74.8 76.2 77.6 79.1 80.5 82.0 83.5 84.918 54.6 56.0 57.4 58.8 60.2 61.6 63.0 64.4 65.8 67.2 68.6 70.1 71.5 73.0 74.4 75.9 77.3 78.8 80.3 81.8 83.3 84.8 86.3 87.819 56.8 58.2 59.6 61.0 62.4 63.9 65.3 66.8 68.2 69.7 71.1 72.6 74.1 75.5 77.0 78.5 80.0 81.5 83.1 84.6 86.1 87.6 89.2 90.720 59.0 60.4 61.9 63.3 64.8 66.3 67.7 69.2 70.7 72.2 73.7 75.2 76.7 78.2 79.8 81.3 82.8 84.4 85.9 87.5 89.1 90.7 92.2 93.821 61.3 62.8 64.3 65.8 67.3 68.8 70.3 71.8 73.3 74.9 76.4 77.9 79.5 81.1 82.6 84.2 85.8 87.4 89.0 90.6 92.2 93.8 95.4 97.122 63.8 65.3 66.8 68.3 69.8 71.4 72.9 74.5 76.1 77.6 79.2 80.8 82.4 84.0 85.6 87.2 88.9 90.5 92.1 93.8 95.5 97.1 98.8 100.523 66.3 67.8 69.4 71.0 72.5 74.1 75.7 77.3 78.9 80.5 82.2 83.8 85.4 87.1 88.7 90.4 92.1 93.8 95.5 97.2 98.9 100.6 102.4 104.124 68.9 70.5 72.1 73.7 75.3 77.0 78.6 80.3 81.9 83.6 85.2 86.9 88.6 90.3 92.0 93.8 95.5 97.2 99.0 100.7 102.5 104.3 106.1 107.925 71.7 73.3 75.0 76.6 78.3 80.0 81.7 83.3 85.1 86.8 88.5 90.2 92.0 93.7 95.5 97.3 99.1 100.9 102.7 104.5 106.3 108.2 110.0 111.926 74.6 76.3 78.0 79.7 81.4 83.1 84.8 86.6 88.4 90.1 91.9 93.7 95.5 97.3 99.2 101.0 102.9 104.7 106.6 108.5 110.4 112.3 114.2 116.227 77.6 79.4 81.1 82.9 84.6 86.4 88.2 90.0 91.9 93.7 95.5 97.4 99.3 101.2 103.1 105.0 106.9 108.8 110.8 112.7 114.7 116.7 118.7 120.728 80.8 82.6 84.4 86.3 88.1 89.9 91.8 93.7 95.6 97.5 99.4 101.3 103.3 105.2 107.2 109.2 111.2 113.2 115.2 117.3 119.3 121.4 123.5 125.629 84.2 86.1 87.9 89.8 91.7 93.7 95.6 97.5 99.5 101.5 103.5 105.5 107.5 109.5 111.6 113.7 115.7 117.8 120.0 122.1 124.2 126.4 128.6 130.830 87.8 89.7 91.7 93.6 95.6 97.6 99.6 101.6 103.7 105.7 107.8 109.9 112.0 114.2 116.3 118.5 120.6 122.8 125.1 127.3 129.5 131.8 134.1 136.431 91.6 93.6 95.6 97.7 99.7 101.8 103.9 106.0 108.2 110.3 112.5 114.7 116.9 119.1 121.4 123.6 125.9 128.2 130.5 132.9 135.3 137.7 140.1 142.532 95.7 97.8 99.9 102.0 104.2 106.3 108.5 110.7 113.0 115.2 117.5 119.8 122.1 124.5 126.8 129.2 131.6 134.0 136.5 139.0 141.5 144.0 146.6 149.133 100.0 102.2 104.4 106.6 108.9 111.2 113.5 115.8 118.2 120.5 122.9 125.4 127.8 130.3 132.8 135.3 137.8 140.4 143.0 145.6 148.3 150.9 153.7 156.434 104.7 107.0 109.3 111.7 114.0 116.4 118.9 121.3 123.8 126.3 128.8 131.4 134.0 136.6 139.2 141.9 144.6 147.4 150.1 152.9 155.7 158.6 161.5 164.435 109.7 112.2 114.6 117.1 119.6 122.2 124.7 127.3 129.9 132.6 135.3 138.0 140.8 143.6 146.4 149.2 152.1 155.0 158.0 161.0 164.0 167.1 170.2 173.336 115.2 117.8 120.4 123.0 125.7 128.4 131.1 133.9 136.7 139.5 142.4 145.3 148.3 151.3 154.3 157.3 160.5 163.6 166.8 170.0 173.3 176.6 179.9 183.337 121.3 124.0 126.8 129.6 132.4 135.3 138.2 141.2 144.2 147.3 150.3 153.5 156.7 159.9 163.1 166.5 169.8 173.2 176.7 180.2 183.7 187.3 191.0 194.738 127.9 130.8 133.8 136.8 139.9 143.0 146.2 149.4 152.6 155.9 159.2 162.6 166.1 169.6 173.2 176.8 180.4 184.2 188.0 191.8 195.7 199.7 203.7 207.739 135.3 138.5 141.7 145.0 148.3 151.7 155.1 158.6 162.1 165.7 169.4 173.1 176.9 180.7 184.7 188.7 192.7 196.8 201.0 205.3 209.6 214.0 218.5 223.040 143.7 147.1 150.6 154.2 157.8 161.5 165.3 169.1 173.0 177.0 181.1 185.2 189.4 193.7 198.1 202.5 207.1 211.7 216.4 221.1 226.0 231.0 236.0 241.141 153.2 157.0 160.9 164.8 168.9 173.0 177.2 181.5 185.8 190.3 194.8 199.5 204.2 209.1 214.0 219.1 224.2 229.4 234.8 240.2 245.8 251.5 257.2 263.142 164.3 168.6 172.9 177.3 181.9 186.5 191.3 196.1 201.1 206.2 211.4 216.7 222.2 227.7 233.4 239.2 245.2 251.3 257.5 263.8 270.3 276.9 283.6 290.543 177.5 182.3 187.3 192.4 197.6 202.9 208.4 214.0 219.8 225.8 231.8 238.1 244.5 251.0 257.7 264.6 271.7 278.9 286.3 293.8 301.5 309.4 317.4 325.744 193.6 199.3 205.1 211.0 217.2 223.5 230.0 236.7 243.6 250.8 258.1 265.6 273.3 281.2 289.4 297.8 306.3 315.1 324.1 333.3 342.8 352.4 362.3 372.445 214.1 220.9 227.9 235.2 242.7 250.4 258.4 266.7 275.3 284.1 293.3 302.6 312.3 322.3 332.5 343.0 353.8 364.9 376.2 387.9 399.8 412.0 424.5 437.446 241.5 250.0 258.9 268.2 277.8 287.8 298.1 308.8 319.9 331.4 343.3 355.5 368.1 381.1 394.5 408.3 422.5 437.1 452.0 467.4 483.3 499.6 516.3 533.547 280.9 292.4 304.4 316.9 330.0 343.6 357.8 372.5 387.7 403.4 419.8 436.6 454.1 472.1 490.7 509.9 529.8 550.4 571.7 593.8 616.7 640.5 665.3 691.048 344.1 360.9 378.4 396.8 416.0 436.0 456.9 478.6 501.2 524.7 549.3 574.8 601.5 629.4 658.6 689.3 721.5 755.6 791.5 829.7 870.4 913.9 960.6 1011.249 461.1 488.4 517.2 547.5 579.4 613.1 648.8 686.7 727.0 770.1 816.4 866.4 920.8 980.4 1046.2 1119.9 1203.3 1299.7 1413.6 1553.1 1732.9 1986.3 2419.6 >2419.6
IDEXX Quanti-Tray/2000

日本語版

Quanti-Tray および Defined Substrate Technology は、 米国および / または他国の IDEXX Laboratories, 

Inc, の商標または登録商標です。 米国特許番号 5,518,892; 5,620,895; and 5,753,456 によって保護されています。他の特許は出願中です。

はじめに

IDEXX Quanti-Tray/2000 は、 IDEXX が特許を取得している Defined Substrate Technology (特定酵素基質法)に基づいたColilertを用い、 検

体 100 mL 中の最確数を測定するシステムです。 Colilert と検体の混合液をQuanti-Tray/2000 に加え、 シーラーで封入し、取扱説明書に従って培

養してください。 その後、陽性ウェル数を数え、添付の最確数 (MPN) 表を用いて、 MPN を判定します。

内容

このパッケージには、滅菌 Quanti-Tray/2000 が 100 個入っています。

テクニカル・サポート

アイデックス ラボラトリーズ株式会社

 TEL:0422-71-5921

www.idexx.co.jp/water

操作手順

1. ウェル側を手のひらに向けて、 トレイ を

垂直に持ってください。

2. トレイ が手のひらに向かって曲がるよう

に、 トレイ の上部を強く握ってくださ

い。

3. トレイのつまみをウェル側から引き離し

て、注入口を広げて下さい。 トレイのつ

まみまたはトレイの内部に触れないように

してください。 

4. トレイのつまみにかからないように直接、

Colilertと検体の混合液を注いでくださ

い。 小さいウェルを 2 ~ 3 回軽くたた

き、気泡を抜いてください。

5. 検体の入った トレイ を シーラーの ゴム

製シートの上に、 ウェル側 を下に向けて

セットしてください。

6. シーラーの 取扱説明書に従って、密封して

ください。

7. Colilertの取扱説明書に従って、培養して

ください。

8. 大小の陽性ウェル数を数え、 添付の最

確数(MPN) 表を使用して MPN を求めて

ください。

9. 検体及びトレイの廃棄は高圧滅菌してく

ださい。

IDEXX Laboratories, Inc.

One IDEXX Drive

Westbrook, Maine 04092 USA
IDEXX Quanti-Tray/2000 MPN 表

大きいウ

ェル陽

性数

小さいウェル陽性数

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

0 <1 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.1 15.1 16.1 17.1 18.1 19.1 20.2 21.2 22.2 23.3 24.31 1.0 2.0 3.0 4.0 5.0 6.0 7.1 8.1 9.1 10.1 11.1 12.1 13.2 14.2 15.2 16.2 17.3 18.3 19.3 20.4 21.4 22.4 23.5 24.5 25.62 2.0 3.0 4.1 5.1 6.1 7.1 8.1 9.2 10.2 11.2 12.2 13.3 14.3 15.4 16.4 17.4 18.5 19.5 20.6 21.6 22.7 23.7 24.8 25.8 26.93 3.1 4.1 5.1 6.1 7.2 8.2 9.2 10.3 11.3 12.4 13.4 14.5 15.5 16.5 17.6 18.6 19.7 20.8 21.8 22.9 23.9 25.0 26.1 27.1 28.24 4.1 5.2 6.2 7.2 8.3 9.3 10.4 11.4 12.5 13.5 14.6 15.6 16.7 17.8 18.8 19.9 21.0 22.0 23.1 24.2 25.3 26.3 27.4 28.5 29.65 5.2 6.3 7.3 8.4 9.4 10.5 11.5 12.6 13.7 14.7 15.8 16.9 17.9 19.0 20.1 21.2 22.2 23.3 24.4 25.5 26.6 27.7 28.8 29.9 31.06 6.3 7.4 8.4 9.5 10.6 11.6 12.7 13.8 14.9 16.0 17.0 18.1 19.2 20.3 21.4 22.5 23.6 24.7 25.8 26.9 28.0 29.1 30.2 31.3 32.47 7.5 8.5 9.6 10.7 11.8 12.8 13.9 15.0 16.1 17.2 18.3 19.4 20.5 21.6 22.7 23.8 24.9 26.0 27.1 28.3 29.4 30.5 31.6 32.8 33.98 8.6 9.7 10.8 11.9 13.0 14.1 15.2 16.3 17.4 18.5 19.6 20.7 21.8 22.9 24.1 25.2 26.3 27.4 28.6 29.7 30.8 32.0 33.1 34.3 35.49 9.8 10.9 12.0 13.1 14.2 15.3 16.4 17.6 18.7 19.8 20.9 22.0 23.2 24.3 25.4 26.6 27.7 28.9 30.0 31.2 32.3 33.5 34.6 35.8 37.0

10 11.0 12.1 13.2 14.4 15.5 16.6 17.7 18.9 20.0 21.1 22.3 23.4 24.6 25.7 26.9 28.0 29.2 30.3 31.5 32.7 33.8 35.0 36.2 37.4 38.6

11 12.2 13.4 14.5 15.6 16.8 17.9 19.1 20.2 21.4 22.5 23.7 24.8 26.0 27.2 28.3 29.5 30.7 31.9 33.0 34.2 35.4 36.6 37.8 39.0 40.2

12 13.5 14.6 15.8 16.9 18.1 19.3 20.4 21.6 22.8 23.9 25.1 26.3 27.5 28.6 29.8 31.0 32.2 33.4 34.6 35.8 37.0 38.2 39.5 40.7 41.913 14.8 16.0 17.1 18.3 19.5 20.6 21.8 23.0 24.2 25.4 26.6 27.8 29.0 30.2 31.4 32.6 33.8 35.0 36.2 37.5 38.7 39.9 41.2 42.4 43.614 16.1 17.3 18.5 19.7 20.9 22.1 23.3 24.5 25.7 26.9 28.1 29.3 30.5 31.7 33.0 34.2 35.4 36.7 37.9 39.1 40.4 41.6 42.9 44.2 45.415 17.5 18.7 19.9 21.1 22.3 23.5 24.7 25.9 27.2 28.4 29.6 30.9 32.1 33.3 34.6 35.8 37.1 38.4 39.6 40.9 42.2 43.4 44.7 46.0 47.316 18.9 20.1 21.3 22.6 23.8 25.0 26.2 27.5 28.7 30.0 31.2 32.5 33.7 35.0 36.3 37.5 38.8 40.1 41.4 42.7 44.0 45.3 46.6 47.9 49.217 20.3 21.6 22.8 24.1 25.3 26.6 27.8 29.1 30.3 31.6 32.9 34.1 35.4 36.7 38.0 39.3 40.6 41.9 43.2 44.5 45.9 47.2 48.5 49.8 51.218 21.8 23.1 24.3 25.6 26.9 28.1 29.4 30.7 32.0 33.3 34.6 35.9 37.2 38.5 39.8 41.1 42.4 43.8 45.1 46.5 47.8 49.2 50.5 51.9 53.219 23.3 24.6 25.9 27.2 28.5 29.8 31.1 32.4 33.7 35.0 36.3 37.6 39.0 40.3 41.6 43.0 44.3 45.7 47.1 48.4 49.8 51.2 52.6 54.0 55.420 24.9 26.2 27.5 28.8 30.1 31.5 32.8 34.1 35.4 36.8 38.1 39.5 40.8 42.2 43.6 44.9 46.3 47.7 49.1 50.5 51.9 53.3 54.7 56.1 57.621 26.5 27.9 29.2 30.5 31.8 33.2 34.5 35.9 37.3 38.6 40.0 41.4 42.8 44.1 45.5 46.9 48.4 49.8 51.2 52.6 54.1 55.5 56.9 58.4 59.922 28.2 29.5 30.9 32.3 33.6 35.0 36.4 37.7 39.1 40.5 41.9 43.3 44.8 46.2 47.6 49.0 50.5 51.9 53.4 54.8 56.3 57.8 59.3 60.8 62.323 29.9 31.3 32.7 34.1 35.5 36.8 38.3 39.7 41.1 42.5 43.9 45.4 46.8 48.3 49.7 51.2 52.7 54.2 55.6 57.1 58.6 60.2 61.7 63.2 64.724 31.7 33.1 34.5 35.9 37.3 38.8 40.2 41.7 43.1 44.6 46.0 47.5 49.0 50.5 52.0 53.5 55.0 56.5 58.0 59.5 61.1 62.6 64.2 65.8 67.325 33.6 35.0 36.4 37.9 39.3 40.8 42.2 43.7 45.2 46.7 48.2 49.7 51.2 52.7 54.3 55.8 57.3 58.9 60.5 62.0 63.6 65.2 66.8 68.4 70.026 35.5 36.9 38.4 39.9 41.4 42.8 44.3 45.9 47.4 48.9 50.4 52.0 53.5 55.1 56.7 58.2 59.8 61.4 63.0 64.7 66.3 67.9 69.6 71.2 72.927 37.4 38.9 40.4 42.0 43.5 45.0 46.5 48.1 49.6 51.2 52.8 54.4 56.0 57.6 59.2 60.8 62.4 64.1 65.7 67.4 69.1 70.8 72.5 74.2 75.928 39.5 41.0 42.6 44.1 45.7 47.3 48.8 50.4 52.0 53.6 55.2 56.9 58.5 60.2 61.8 63.5 65.2 66.9 68.6 70.3 72.0 73.7 75.5 77.3 79.029 41.7 43.2 44.8 46.4 48.0 49.6 51.2 52.8 54.5 56.1 57.8 59.5 61.2 62.9 64.6 66.3 68.0 69.8 71.5 73.3 75.1 76.9 78.7 80.5 82.430 43.9 45.5 47.1 48.7 50.4 52.0 53.7 55.4 57.1 58.8 60.5 62.2 64.0 65.7 67.5 69.3 71.0 72.9 74.7 76.5 78.3 80.2 82.1 84.0 85.931 46.2 47.9 49.5 51.2 52.9 54.6 56.3 58.1 59.8 61.6 63.3 65.1 66.9 68.7 70.5 72.4 74.2 76.1 78.0 79.9 81.8 83.7 85.7 87.6 89.632 48.7 50.4 52.1 53.8 55.6 57.3 59.1 60.9 62.7 64.5 66.3 68.2 70.0 71.9 73.8 75.7 77.6 79.5 81.5 83.5 85.4 87.5 89.5 91.5 93.633 51.2 53.0 54.8 56.5 58.3 60.2 62.0 63.8 65.7 67.6 69.5 71.4 73.3 75.2 77.2 79.2 81.2 83.2 85.2 87.3 89.3 91.4 93.6 95.7 97.834 53.9 55.7 57.6 59.4 61.3 63.1 65.0 67.0 68.9 70.8 72.8 74.8 76.8 78.8 80.8 82.9 85.0 87.1 89.2 91.4 93.5 95.7 97.9 100.2 102.435 56.8 58.6 60.5 62.4 64.4 66.3 68.3 70.3 72.3 74.3 76.3 78.4 80.5 82.6 84.7 86.9 89.1 91.3 93.5 95.7 98.0 100.3 102.6 105.0 107.336 59.8 61.7 63.7 65.7 67.7 69.7 71.7 73.8 75.9 78.0 80.1 82.3 84.5 86.7 88.9 91.2 93.5 95.8 98.1 100.5 102.9 105.3 107.7 110.2 112.737 62.9 65.0 67.0 69.1 71.2 73.3 75.4 77.6 79.8 82.0 84.2 86.5 88.8 91.1 93.4 95.8 98.2 100.6 103.1 105.6 108.1 110.7 113.3 115.9 118.638 66.3 68.4 70.6 72.7 74.9 77.1 79.4 81.6 83.9 86.2 88.6 91.0 93.4 95.8 98.3 100.8 103.4 105.9 108.6 111.2 113.9 116.6 119.4 122.2 125.039 70.0 72.2 74.4 76.7 78.9 81.3 83.6 86.0 88.4 90.9 93.4 95.9 98.4 101.0 103.6 106.3 109.0 111.8 114.6 117.4 120.3 123.2 126.1 129.2 132.240 73.8 76.2 78.5 80.9 83.3 85.7 88.2 90.8 93.3 95.9 98.5 101.2 103.9 106.7 109.5 112.4 115.3 118.2 121.2 124.3 127.4 130.5 133.7 137.0 140.341 78.0 80.5 83.0 85.5 88.0 90.6 93.3 95.9 98.7 101.4 104.3 107.1 110.0 113.0 116.0 119.1 122.2 125.4 128.7 132.0 135.4 138.8 142.3 145.9 149.542 82.6 85.2 87.8 90.5 93.2 96.0 98.8 101.7 104.6 107.6 110.6 113.7 116.9 120.1 123.4 126.7 130.1 133.6 137.2 140.8 144.5 148.3 152.2 156.1 160.243 87.6 90.4 93.2 96.0 99.0 101.9 105.0 108.1 111.2 114.5 117.8 121.1 124.6 128.1 131.7 135.4 139.1 143.0 147.0 151.0 155.2 159.4 163.8 168.2 172.844 93.1 96.1 99.1 102.2 105.4 108.6 111.9 115.3 118.7 122.3 125.9 129.6 133.4 137.4 141.4 145.5 149.7 154.1 158.5 163.1 167.9 172.7 177.7 182.9 188.245 99.3 102.5 105.8 109.2 112.6 116.2 119.8 123.6 127.4 131.4 135.4 139.6 143.9 148.3 152.9 157.6 162.4 167.4 172.6 178.0 183.5 189.2 195.1 201.2 207.546 106.3 109.8 113.4 117.2 121.0 125.0 129.1 133.3 137.6 142.1 146.7 151.5 156.5 161.6 167.0 172.5 178.2 184.2 190.4 196.8 203.5 210.5 217.8 225.4 233.347 114.3 118.3 122.4 126.6 130.9 135.4 140.1 145.0 150.0 155.3 160.7 166.4 172.3 178.5 185.0 191.8 198.9 206.4 214.2 222.4 231.0 240.0 249.5 259.5 270.048 123.9 128.4 133.1 137.9 143.0 148.3 153.9 159.7 165.8 172.2 178.9 186.0 193.5 201.4 209.8 218.7 228.2 238.2 248.9 260.3 272.3 285.1 298.7 313.0 328.249 135.5 140.8 146.4 152.3 158.5 165.0 172.0 179.3 187.2 195.6 204.6 214.3 224.7 235.9 248.1 261.3 275.5 290.9 307.6 325.5 344.8 365.4 387.3 410.6 435.2

Quanti-Tray および Defined Substrate Technology は、米国および / または他国の IDEXX Laboratories, Inc. の商標または登録商標です。米国特許番号 4.925.789; 5.429.933; 5.518.892 によって保護されています。他の特許は出願中です。
IDEXX Quanti-Tray/2000 MPN 表

大きいウ

ェル陽

性数

小さいウェル陽性数

25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48

0 25.3 26.4 27.4 28.4 29.5 30.5 31.5 32.6 33.6 34.7 35.7 36.8 37.8 38.9 40.0 41.0 42.1 43.1 44.2 45.3 46.3 47.4 48.5 49.51 26.6 27.7 28.7 29.8 30.8 31.9 32.9 34.0 35.0 36.1 37.2 38.2 39.3 40.4 41.4 42.5 43.6 44.7 45.7 46.8 47.9 49.0 50.1 51.22 27.9 29.0 30.0 31.1 32.2 33.2 34.3 35.4 36.5 37.5 38.6 39.7 40.8 41.9 43.0 44.0 45.1 46.2 47.3 48.4 49.5 50.6 51.7 52.83 29.3 30.4 31.4 32.5 33.6 34.7 35.8 36.8 37.9 39.0 40.1 41.2 42.3 43.4 44.5 45.6 46.7 47.8 48.9 50.0 51.2 52.3 53.4 54.54 30.7 31.8 32.8 33.9 35.0 36.1 37.2 38.3 39.4 40.5 41.6 42.8 43.9 45.0 46.1 47.2 48.3 49.5 50.6 51.7 52.9 54.0 55.1 56.35 32.1 33.2 34.3 35.4 36.5 37.6 38.7 39.9 41.0 42.1 43.2 44.4 45.5 46.6 47.7 48.9 50.0 51.2 52.3 53.5 54.6 55.8 56.9 58.16 33.5 34.7 35.8 36.9 38.0 39.2 40.3 41.4 42.6 43.7 44.8 46.0 47.1 48.3 49.4 50.6 51.7 52.9 54.1 55.2 56.4 57.6 58.7 59.97 35.0 36.2 37.3 38.4 39.6 40.7 41.9 43.0 44.2 45.3 46.5 47.7 48.8 50.0 51.2 52.3 53.5 54.7 55.9 57.1 58.3 59.4 60.6 61.88 36.6 37.7 38.9 40.0 41.2 42.3 43.5 44.7 45.9 47.0 48.2 49.4 50.6 51.8 53.0 54.1 55.3 56.5 57.7 59.0 60.2 61.4 62.6 63.89 38.1 39.3 40.5 41.6 42.8 44.0 45.2 46.4 47.6 48.8 50.0 51.2 52.4 53.6 54.8 56.0 57.2 58.4 59.7 60.9 62.1 63.4 64.6 65.8

10 39.7 40.9 42.1 43.3 44.5 45.7 46.9 48.1 49.3 50.6 51.8 53.0 54.2 55.5 56.7 57.9 59.2 60.4 61.7 62.9 64.2 65.4 66.7 67.9

11 41.4 42.6 43.8 45.0 46.3 47.5 48.7 49.9 51.2 52.4 53.7 54.9 56.1 57.4 58.6 59.9 61.2 62.4 63.7 65.0 66.3 67.5 68.8 70.1

12 43.1 44.3 45.6 46.8 48.1 49.3 50.6 51.8 53.1 54.3 55.6 56.8 58.1 59.4 60.7 62.0 63.2 64.5 65.8 67.1 68.4 69.7 71.0 72.413 44.9 46.1 47.4 48.6 49.9 51.2 52.5 53.7 55.0 56.3 57.6 58.9 60.2 61.5 62.8 64.1 65.4 66.7 68.0 69.3 70.7 72.0 73.3 74.714 46.7 48.0 49.3 50.5 51.8 53.1 54.4 55.7 57.0 58.3 59.6 60.9 62.3 63.6 64.9 66.3 67.6 68.9 70.3 71.6 73.0 74.4 75.7 77.115 48.6 49.9 51.2 52.5 53.8 55.1 56.4 57.8 59.1 60.4 61.8 63.1 64.5 65.8 67.2 68.5 69.9 71.3 72.6 74.0 75.4 76.8 78.2 79.616 50.5 51.8 53.2 54.5 55.8 57.2 58.5 59.9 61.2 62.6 64.0 65.3 66.7 68.1 69.5 70.9 72.3 73.7 75.1 76.5 77.9 79.3 80.8 82.217 52.5 53.9 55.2 56.6 58.0 59.3 60.7 62.1 63.5 64.9 66.3 67.7 69.1 70.5 71.9 73.3 74.8 76.2 77.6 79.1 80.5 82.0 83.5 84.918 54.6 56.0 57.4 58.8 60.2 61.6 63.0 64.4 65.8 67.2 68.6 70.1 71.5 73.0 74.4 75.9 77.3 78.8 80.3 81.8 83.3 84.8 86.3 87.819 56.8 58.2 59.6 61.0 62.4 63.9 65.3 66.8 68.2 69.7 71.1 72.6 74.1 75.5 77.0 78.5 80.0 81.5 83.1 84.6 86.1 87.6 89.2 90.720 59.0 60.4 61.9 63.3 64.8 66.3 67.7 69.2 70.7 72.2 73.7 75.2 76.7 78.2 79.8 81.3 82.8 84.4 85.9 87.5 89.1 90.7 92.2 93.821 61.3 62.8 64.3 65.8 67.3 68.8 70.3 71.8 73.3 74.9 76.4 77.9 79.5 81.1 82.6 84.2 85.8 87.4 89.0 90.6 92.2 93.8 95.4 97.122 63.8 65.3 66.8 68.3 69.8 71.4 72.9 74.5 76.1 77.6 79.2 80.8 82.4 84.0 85.6 87.2 88.9 90.5 92.1 93.8 95.5 97.1 98.8 100.523 66.3 67.8 69.4 71.0 72.5 74.1 75.7 77.3 78.9 80.5 82.2 83.8 85.4 87.1 88.7 90.4 92.1 93.8 95.5 97.2 98.9 100.6 102.4 104.124 68.9 70.5 72.1 73.7 75.3 77.0 78.6 80.3 81.9 83.6 85.2 86.9 88.6 90.3 92.0 93.8 95.5 97.2 99.0 100.7 102.5 104.3 106.1 107.925 71.7 73.3 75.0 76.6 78.3 80.0 81.7 83.3 85.1 86.8 88.5 90.2 92.0 93.7 95.5 97.3 99.1 100.9 102.7 104.5 106.3 108.2 110.0 111.926 74.6 76.3 78.0 79.7 81.4 83.1 84.8 86.6 88.4 90.1 91.9 93.7 95.5 97.3 99.2 101.0 102.9 104.7 106.6 108.5 110.4 112.3 114.2 116.227 77.6 79.4 81.1 82.9 84.6 86.4 88.2 90.0 91.9 93.7 95.5 97.4 99.3 101.2 103.1 105.0 106.9 108.8 110.8 112.7 114.7 116.7 118.7 120.728 80.8 82.6 84.4 86.3 88.1 89.9 91.8 93.7 95.6 97.5 99.4 101.3 103.3 105.2 107.2 109.2 111.2 113.2 115.2 117.3 119.3 121.4 123.5 125.629 84.2 86.1 87.9 89.8 91.7 93.7 95.6 97.5 99.5 101.5 103.5 105.5 107.5 109.5 111.6 113.7 115.7 117.8 120.0 122.1 124.2 126.4 128.6 130.830 87.8 89.7 91.7 93.6 95.6 97.6 99.6 101.6 103.7 105.7 107.8 109.9 112.0 114.2 116.3 118.5 120.6 122.8 125.1 127.3 129.5 131.8 134.1 136.431 91.6 93.6 95.6 97.7 99.7 101.8 103.9 106.0 108.2 110.3 112.5 114.7 116.9 119.1 121.4 123.6 125.9 128.2 130.5 132.9 135.3 137.7 140.1 142.532 95.7 97.8 99.9 102.0 104.2 106.3 108.5 110.7 113.0 115.2 117.5 119.8 122.1 124.5 126.8 129.2 131.6 134.0 136.5 139.0 141.5 144.0 146.6 149.133 100.0 102.2 104.4 106.6 108.9 111.2 113.5 115.8 118.2 120.5 122.9 125.4 127.8 130.3 132.8 135.3 137.8 140.4 143.0 145.6 148.3 150.9 153.7 156.434 104.7 107.0 109.3 111.7 114.0 116.4 118.9 121.3 123.8 126.3 128.8 131.4 134.0 136.6 139.2 141.9 144.6 147.4 150.1 152.9 155.7 158.6 161.5 164.435 109.7 112.2 114.6 117.1 119.6 122.2 124.7 127.3 129.9 132.6 135.3 138.0 140.8 143.6 146.4 149.2 152.1 155.0 158.0 161.0 164.0 167.1 170.2 173.336 115.2 117.8 120.4 123.0 125.7 128.4 131.1 133.9 136.7 139.5 142.4 145.3 148.3 151.3 154.3 157.3 160.5 163.6 166.8 170.0 173.3 176.6 179.9 183.337 121.3 124.0 126.8 129.6 132.4 135.3 138.2 141.2 144.2 147.3 150.3 153.5 156.7 159.9 163.1 166.5 169.8 173.2 176.7 180.2 183.7 187.3 191.0 194.738 127.9 130.8 133.8 136.8 139.9 143.0 146.2 149.4 152.6 155.9 159.2 162.6 166.1 169.6 173.2 176.8 180.4 184.2 188.0 191.8 195.7 199.7 203.7 207.739 135.3 138.5 141.7 145.0 148.3 151.7 155.1 158.6 162.1 165.7 169.4 173.1 176.9 180.7 184.7 188.7 192.7 196.8 201.0 205.3 209.6 214.0 218.5 223.040 143.7 147.1 150.6 154.2 157.8 161.5 165.3 169.1 173.0 177.0 181.1 185.2 189.4 193.7 198.1 202.5 207.1 211.7 216.4 221.1 226.0 231.0 236.0 241.141 153.2 157.0 160.9 164.8 168.9 173.0 177.2 181.5 185.8 190.3 194.8 199.5 204.2 209.1 214.0 219.1 224.2 229.4 234.8 240.2 245.8 251.5 257.2 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