TMTrademark of Dow AgroSciences LLC

308/2E

June 27, 2011

Diane Sinkowski

Senior Environmental Protection Specialist

USDA/APHIS/BRS

4700 River Road, Unit 147

Riverdale, MD 20737

DAS-68416-4 Soybean

Dow AgroSciences Petition Number 09-349-01p for a Determination of Non-regulated Status for Herbicide Tolerant 

DAS-68416-4 soybean

SUPPLEMENTARY DOCUMENTATION IN SUPPORT OF DRAFT ENVIRONMENTAL 

ASSESSMENT

Dear Ms. Sinkowski:

Dow AgroSciences is submitting the following documents to the USDA/APHIS/BRS as supporting material to be 

reviewed and included in the drafting of the Environmental Assessment for DAS-68416-4 soybean. The documents 

are included here as hard copy and on a compact disc (with the exception of the Enlist Weed Control System: 

Technical Bulletin).

Volume/Classification

Volume 1

Volume 2

Volume 3 Supplementary Documents

Title Submitter/Author

1 Regulatory Overview of 2,4-D and Glufosinate Use on DAS-68416-4 Soybean M.S. Krieger

2 EnlistTM Weed Control System: Technical Bulletin M.S. Krieger/Dow 

AgroSciences LLC.

3 EnlistTM Weed Control System Stewardship Program Fact Sheet M.S. Krieger/Dow 

AgroSciences LLC.

4 Stewardship of Herbicide Tolerant Trait Technology for DAS-68416-4 Soybean M.S. Krieger

5 Economic and Agronomic Impacts of the Introduction of DAS-68416-4 Soybean on 

Glyphosate Resistant Weeds in the U.S. Cropping System M.S. Krieger

6 GF-2654 TS Draft Label (submitted as part of an application to U.S.-EPA to register 

the use of a new end-use formulation of 2,4-D on DAS-68416-4 soybean)

M.S. Krieger/Dow 

AgroSciences LLC.

7 Robust crop resistance to broadleaf and grass herbicides provided by 

aryloxyalkanoate dioxygenase transgenes

M.S. Krieger/Terry R. 

Wright et al

8 2,4-Dichlorophenoxyacetic acid (2,4-D)–resistant crops and the potential for evolution 

of 2,4-D–resistant weeds

M.S. Krieger/John F. 

Egan et al

9 Reply to Egan et al.: Stewardship for herbicide-resistance crop technology M.S. Krieger/Terry R. 

Wright et al

10

Utility of Aryloxyalkanoate Dioxygenase Transgenes for Development of New 

Herbicide Resistant Crop Technologies 

http://www.isb.vt.edu/

M.S. Krieger/M.A. 

Peterson et al

Content

Transmittal Document (this letter)

CD containing the following: 

- The supplementary documents 1, 3, 4, 5, 6, 7, 8, 9, 10

- Transmittal Document
DAS-68416-4 Soybean

Dow AgroSciences Petition Number 09-349-01p for a Determination of Non-regulated Status for Herbicide Tolerant DAS-68416-4 Soybean

SUPPLEMENTARY DOCUMENTATION IN SUPPORT OF DRAFT ENVIRONMENTAL ASSESSMENT

June 27, 2011

Page 2

In addition, the table below provides a summary of documents submitted to date and their context with respect to 

DAS-68416-4 soybean.

Context re: DAS-68416-4 Deregulation

1

Regulatory Overview of 

2,4-D and Glufosinate Use 

on DAS-68416-4 Soybean

This document provides information pertaining to the regulation of the use of 2,4-D and glufosinate on DAS 

68416-4 soybean. This information includes: 

i/ Description of the responsibilities of various agencies of the U.S. government with respect to pesticide 

registration and use. 

ii/ Comparison of the current and proposed new use patterns for 2,4-D soybean.

iii/ Evaluation of the current environmental risk assessments for 2,4-D and glufosinate relative to the proposed 

new use patterns.

2

EnlistTM Weed Control 

System: Technical 

Bulletin

This document provides an technical overview of the EnlistTM Weed Control System that includes DAS-68416-4 

soybean and the associated herbicide solution centered on the proprietary choline salt of 2,4-D. 

3

EnlistTM Weed Control 

System Stewardship 

Program Overview

This document outlines the EnlistTM Weed Control System stewardship program including goals, components 

and training.

4

Robust crop resistance to 

broadleaf and grass 

herbicides provided by 

aryloxyalkanoate 

dioxygenase transgenes

This manuscript in the Proceedings of the National Academy of Sciences, describes a class of bacterial enzymes 

[aryloxyalkanoate dioxygenases (AADs)] that can provide robust field resistance to 2,4-D when expressed in 

maize and soybean (e.g. DAS-68416-4) crops. These attributes indicate the utility of these types of enzymes for 

herbicide-resistant crop applications. They may offer growers options to overcome the significant developing 

issues of glyphosate resistant weeds. 

5

2,4-

Dichlorophenoxyacetic 

acid (2,4-D)–resistant 

crops and the potential for 

evolution of 2,4-

D–resistant weeds

A letter to the editor of the Proceedings of the National Academy of Sciences relating to the conclusions in the 

manuscript (Document #4) above.

6

Reply to Egan et al.: 

Stewardship for herbicideresistance crop 

technology

A reply to editor of the Proceedings of the National Academy of Sciences to the letter to the editor in document 

#5 from the authors of Document #4

7

Economic and Agronomic 

Impacts of the 

Introduction of DAS68416-4 Soybean on 

Glyphosate Resistant 

Weeds in the U.S. 

Cropping System

This document provides information pertaining to the economic and agronomic impact of DAS-68416-4 

Soybean. This information includes: 

i/ Benefits of Glyphosate Tolerant Crops

ii/ Weed Management in Glyphosate Tolerant Corn and Soybeans

iii/ Long-term Weed Resistance Management through Use of Herbicide Tolerant DAS-68416-4 Soybean

iv/ Economic Benefits of Dow AgroSciences Herbicide Tolerant Technology

v/ Impact of 2,4-D on Herbicide Tolerant DAS-40278-9 Corn and DAS-68416-4 Soybeans on the Cost and 

Environmental Load of Herbicides Applied on Glyphosate Tolerant Corn and Soybeans 

8

Stewardship of Herbicide 

Tolerant Trait Technology 

for DAS-68416-4 

Soybean

This document provides detailed information pertaining to Dow AgroSciences’ comprehensive stewardship 

program for DAS-68416-4 Soybean and its associated new herbicide technology, 2,4-D choline salt. 

9 GF-2654 TS Draft Label Draft label submitted as part of an application to U.S.-EPA to register the use of a new end-use formulation of 

2,4-D on DAS-68416-4 Soybean

10

Utility of 

Aryloxyalkanoate 

Dioxygenase Transgenes 

for Development of New 

Herbicide Resistant Crop 

Technologies

Manuscript in the Agricultural and Environmental Biotechnology News Report, describes the utility of 

aryloxyalkanoate dioxygenase transgenes as herbicide resistant traits (e.g. DAS-68416-4 Soybean) that will 

provide crop producers with new tools for managing weeds and herbicide resitance. 

Document
DAS-68416-4 Soybean

Dow AgroSciences Petition Number 09-349-01p for a Determination of Non-regulated Status for Herbicide Tolerant DAS-68416-4 Soybean

SUPPLEMENTARY DOCUMENTATION IN SUPPORT OF DRAFT ENVIRONMENTAL ASSESSMENT

June 27, 2011

Page 3

If you should have any questions or require further information, please contact me at 317-337-3692.

Sincerely,

Gregory L. Orr, Ph.D.

Global Regulatory Leader 

317-337-3692

317-337-4649 (fax)

glorr@dow.com

Attachments
DAS-68416-4 Soybean

Dow AgroSciences Petition Number 09-349-01p for a Determination of Non-regulated Status for Herbicide Tolerant DAS-68416-4 

Soybean

SUPPLEMENTARY DOCUMENTATION IN SUPPORT OF DRAFT ENVIRONMENTAL ASSESSMENT

June 27, 2011

Page 4

DISTRIBUTION PAGE

DED Code: ____D1C__________________

bcc: Mark Krieger, 308/2E

Greg Orr, 308/2E

Stephanie Burton, 308/2C

Federal Action Files, 308/2E unstapled