TM T ra demark of Dow AgroSciences LLC 

### 308/2E June 27, 2011

Diane Sinkowski Senior Environmental Protection Specialist USDA/APHIS/BRS 4700 River Road, Unit 147 Riverdale, MD 20737 DAS

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- 01p for a Determination of Non

- regulated Status for Herbicid e Tolerant DAS

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- 4 soybean SUPPLEMENTARY DOCUMENTATION IN SUPPORT OF DRAFT ENVIRONMENTAL ASSESSMENT Dear Ms. Sinkowski : Dow AgroSciences is submitting the following do cuments to the USDA/APHIS/BRS as supporting material to be reviewed and included in the drafting of the Environmental Assessment for DAS

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- 4 soybean . The documents are included here as hard copy and on a compact disc (with the exception of the Enlist Weed Control System: Technical Bulletin)

. Volume/Classification Volume 1

Volume 2

Volume 3 Supplementary Documents Title Submitter/Author 1 Regulatory Overview of 2,4-D and Glufosinate Use on DAS-68416-4 Soybean M.S. Krieger 2 EnlistTM Weed Control System: Technical Bulletin M.S. Krieger/Dow AgroSciences LLC. 3 EnlistTM Weed Control System Stewardship Program Fact Sheet M.S. Krieger/Dow AgroSciences LLC. 4 Stewardship of Herbicide Tolerant Trait Technology for DAS-68416-4 Soybean M.S. Krieger 5 Economic and Agronomic Impacts of the Introduction of DAS-68416-4 Soybean on Glyphosate Resistant Weeds in the U.S. Cropping System M.S. Krieger
6 GF-2654 TS Draft Label (submitted as part of an application to U.S.-EPA to register the use of a new end-use formulation of 2,4-D on DAS-68416-4 soybean) M.S. Krieger/Dow AgroSciences LLC. 7 Robust crop resistance to broadleaf and grass herbicides provided by aryloxyalkanoate dioxygenase transgenes M.S. Krieger/Terry R. Wright et al 8 2,4-Dichlorophenoxyacetic acid (2,4-D)–resistant crops and the potential for evolution of 2,4-D–resistant weeds M.S. Krieger/John F. Egan et al 9 Reply to Egan et al.: Stewardship for herbicide-resistance crop technology M.S. Krieger/Terry R. Wright et al 10 Utility of Aryloxyalkanoate Dioxygenase Transgenes for Development of New Herbicide Resistant Crop Technologies http://www.isb.vt.edu/ M.S. Krieger/M.A. Peterson et al Content Transmittal Document (this letter) CD containing the following: - The supplementary documents 1, 3, 4, 5, 6, 7, 8, 9, 10 - Transmittal DocumentDAS

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### In addition, the table below provides a summary of documents submitted to date and their context with respect to DAS

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. Context re: DAS-68416-4 Deregulation

1 Regulatory Overview of 2,4-D and Glufosinate Use on DAS-68416-4 Soybean This document provides information pertaining to the regulation of the use of 2,4-D and glufosinate on DAS 68416-4 soybean. This information includes: i/ Description of the responsibilities of various agencies of the U.S. government with respect to pesticide registration and use. ii/ Comparison of the current and proposed new use patterns for 2,4-D soybean. iii/ Evaluation of the current environmental risk assessments for 2,4-D and glufosinate relative to the proposed new use patterns. 2 EnlistTM Weed Control System: Technical Bulletin This document provides an technical overview of the Enlist TM Weed Control System that includes DAS-68416-4 soybean and the associated herbicide solution centered on the proprietary choline salt of 2,4-D.
3 EnlistTM Weed Control System Stewardship Program Overview This document outlines the Enlist TM Weed Control System stewardship program including goals, components and training.

4 Robust crop resistance to broadleaf and grass herbicides provided by aryloxyalkanoate dioxygenase transgenes This manuscript in the Proceedings of the National Academy of Sciences, describes a class of bacterial enzymes [aryloxyalkanoate dioxygenases (AADs)] that can provide robust field resistance to 2,4-D when expressed in maize and soybean (e.g. DAS-68416-4) crops. These attributes indicate the utility of these types of enzymes for herbicide-resistant crop applications. They may offer growers options to overcome the significant developing issues of glyphosate resistant weeds.

5 2,4- Dichlorophenoxyacetic acid (2,4-D)–resistant crops and the potential for evolution of 2,4- D–resistant weeds A letter to the editor of the Proceedings of the National Academy of Sciences relating to the conclusions in the manuscript (Document #4) above.

6 Reply to Egan et al.:Stewardship for herbicideresistance crop technology A reply to editor of the Proceedings of the National Academy of Sciences to the letter to the editor in document #5 from the authors of Document #4

7 Economic and Agronomic Impacts of theIntroduction of DAS68416-4 Soybean on Glyphosate Resistant Weeds in the U.S. Cropping System This document provides information pertaining to the economic and agronomic impact of DAS-68416-4 Soybean. This information includes: i/ Benefits of Glyphosate Tolerant Crops ii/ Weed Management in Glyphosate Tolerant Corn and Soybeans iii/ Long-term Weed Resistance Management through Use of Herbicide Tolerant DAS-68416-4 Soybean iv/ Economic Benefits of Dow AgroSciences Herbicide Tolerant Technology v/ Impact of 2,4-D on Herbicide Tolerant DAS-40278-9 Corn and DAS-68416-4 Soybeans on the Cost and Environmental Load of Herbicides Applied on Glyphosate Tolerant Corn and Soybeans

8 Stewardship of Herbicide Tolerant Trait Technology for DAS-68416-4 Soybean This document provides detailed information pertaining to Dow AgroSciences’ comprehensive stewardship program for DAS-68416-4 Soybean and its associated new herbicide technology, 2,4-D choline salt.
9 GF-2654 TS Draft Label Draft label submitted as part of an application to U.S.-EPA to register the use of a new end-use formulation of 2,4-D on DAS-68416-4 Soybean



### 10 Utility of Aryloxyalkanoate Dioxygenase Transgenes for Development of New Herbicide Resistant Crop Technologies Manuscript in the Agricultural and Environmental Biotechnology News Report, describes the utility of aryloxyalkanoate dioxygenase transgenes as herbicide resistant traits (e.g. DAS-68416-4 Soybean) that will provide crop producers with new tools for managing weeds and herbicide resitance. DocumentDAS

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### If you should have any questions or require further information, please contact me at 317

- 337

- 3692. Sincerely,

Gregory L. Orr, Ph.D. Global Regulatory Leader 317

- 337

- 3692 317

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- 4649 (fax) glorr@dow.com

AttachmentsDAS

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