### Related cas e s : H ealth related cases : Ecotox related cases : C on c e1 ·n l evel s: 

### T y pe of C oncern: Le v el of C oncern: Health 2 

### Pers i s tence 2



### E xpo s ure Bas ed Review: 

### SAT Report PMN Nu m ber : P-15-0326 SAT Date : 3 / 12 / 2015 Pr int D ate: 717/2015

E co C omments

2 

### es

Bioaccum 1 

### Toxicity 2 

### C omment s

Health: No Expo s ure bas ed te s ting (health) : The equivale n t of the base set h as aheady been s u b mi tted Ecotox: Yes Route s of expo s ure: Keyword s : Health: Inhalatio n Ec otox: All r eleases to water Fate: ; Ke y word s : NEURO UNCERT-C ARD IAC S E NS AQUATOX

- A , C S ummary of A ss e ss ment: Fate:

Fate S ummary : P- 15

- 0326 FATE: S = 

### 778.9 mg/L 

### at 25 o c 

### (M) VP 

### = 14 59 ton at 25 o c 

### (M) 

### BP = 

### 6.5 o c 

### (NOMOS) H = 

### 2.11E-1 (E ) l og Koc 

### = 2 .79 (E)

### l og Fi sh BCF = 1.60 (E) 

### l og Fi sh BAF = 1.92 (E) POTW removal (%) 

### = 90 v ia sorp tion and st rippin g Time for comp l ete ultim ate aer obic biodeg = mo S01pt i on to soils/ sediment s = moderate Volati li zation half-life from a standa r d ri ver = 1 hr s Volati li zation half-life from a standa r d lake = 5 da Atmospheri c Oxidation H alf-lif e = 3 . 9 hr via OH r adic al Atmospheri c Oxidation H alf-lif e = 160 hr via ozo ne PBT Potential: P2B 1 *CE B F ATE: Migration to gro und water = moderate 

### Health: Health**Sum~** 

### through the s kin and GI u·act under ordinat y condi tio n s because the- expect good abs01pti on via the lungs (pc h e m). B ased on t he s ubmi tted studies, there is co n cem f o r neurotoxici ty and un cetta in co n cem for ca rdi ac sen sit iz atio n. There i s also co n cem for analogy to

acti vati on Negative for c hro m oso me abenations in human peripheral blood lymphocytes wit h and wi th out ac ti vati on , in viu·o Negative in a micronucleus assay , 28 -da y inh alatio n st u dy in rats Acute inh alatio n st u dy in rats, 1-hr (33,000 ppm)- no deaths, decreased ac ti vity l eve l s after 5 minute s of exposure ; u·emors , mu scle spas m s and co n v ul sio n s after 10 minutes of expos ure ; study tenninated after 10 minute s of exposure Acute inh alatio n st u dy in rats, 4-hr ( 1 7,000, 25,000 ppm)- hi gh dose gro up termin ate d after h av ing mu s cl e s p asms at 5 minute s and viole n t co nvul s i on s after 8 minutes ; d ecreased activity in the low dose gr o up Acute inh alatio n st u dy in rats, 4-hr n ose - onl y - deaths at 24 , 000 ppm and greater; dose gr oup s at 50 , 000 ppm and gre ater were ten nin ated wi thin t he fir st few minutes due t o humane reasons ; n o deaths o r clinical signs of t oxi city at 15 , 000 ppm Acute inh alatio n st u dy in rats, 1-hr n o deaths at 24 , 000 ppm Ca rdi ac sen s i tizati on s tud y in dogs (70,000, 80,000, 90,000 ppm) -NOEL= 70,000 ppm ; co nvul sio n s in all dose gr o up s ; early tennination of s tud y at 80,000 and 90 , 000 ppm (termin ated befor e c h alle n ge dose) 28

- day inh alati on s tud y in r ats (1000 , 10 , 000 , 20 , 000/ 15 ,000 ppm)- 4 of 10 m al es died in t he hi gh dose g r oup , NOAEC = 10 , 000 ppm

### Ecotox: Te s t Te s t Te s t End Predicted Mea s ured C omments Organi s m Type Point fi s h 96-h L C 50 20 76 . 1 daphnid 48-h LC50 12 22 . 5 green algal 96-h E C 50 13 > 23 .7 fi s h chronic 2 . 2



## - value daphnid



## - chronic 1.5 2 . 25 v alue algal



## - chronic 4 . 1 6 . 9 v alue**ewag~** 

### 3-h E C 50



## -**ludg~ ewag~**  

## - C hronic



## -**lud ~**  

### Value Ec otox V alue s C omments : Pr edic t io n s are based o n SARs f o r SAR c h emical cl ass = MW IIl og K ow = wit h m p = - 90 . 5 C at 5 C (P ) ; p H 7 ; effective co n ce n t r ati o n s based o n 100% ac ti ve ingredients and n ominal concen u·atio n s; h a r dness < 1 50 . 0 m g/L as CaC03 ; and T OC < 2 . 0 m g/L ;

was statem ent p r ovided . The s t u dy was co n du cted in Jl m e 20 11 acco r ding to t h e OECD Gu ide lin es f o r Test ing o f Chemicals n o . 203 "Fis h Ac ute Toxicity T es t (1992) .Medaka (0 1 yzias latip es) were exposed to t h e P MN m aterial for 96 h o ur s unde r semi- s tatic co nditi o n s wit h t he test m aterial repleni s hed eve1y 24 h ours . Ten fi s h p e r co n cent rati o n wer e expo se d to the PMN m ateri al wi th 99 . 976% purity unde r a 16-h o ur li g h t: 8 -ho m· dark cy cl e. An initial range fmding t est was perf01med wi th 0, 10 , 100 and 500 mg/L test co n centratio n s. Mea s ured co n cen t r atio n s wer e much lower than n ominal due to volati li ty of the t est material (V P = 453 mm H g). A seco nd ran ge-fmding t est was perf01med u s ing 0, 50, and 250 mg/L test co n cent rati o n s but t he measured co n cent rati o n s were e qu a lly low t o t h ose from the fir s t range-fmding s tudy. For the defmi t ive t est, the t emperature ran ged from 23 .7

- 2 4 . 1 ° C , DO= 6.6 - 8.2 mg/L , pH= 7.2

- 7.6. The mean , mea s ured te s t co n centratio n s were 0 , 13 . 1 , 20.5 , 34.4 , 55.2 , and 105 m g/L all of w hi c h wer e 2 1 - 78% of n o minal (0 , 25 , 44 , 79 , 140 , and 250 m g/L) . T e s t co n cen t r atio n s were sa mpl ed at two renewal time s re s ulting in a set of four sam ple s take n durin g the 96 -hour expo s ure p e ri od . Due to the volatility of the te s t m ateri al , te s t co n centratio n s were dete1mined at hi g h e r than t he upper limit co n cent rati o n t h at t he guideline s u ggests 

### ( > 100 m g/L) . Analy s i s of the t est m ateri al was p e rf o nn ed at the beginning and end of each r enewal period . Each te s t g r o up was co ll ected and analyzed by GC/MS w i t h a de t ec t ion limi t ( LOD ) = 0 . 00005 m g/ L. No mortality was obser ved in the cont r ol g r o up . In the 13 . 1 , 20 . 5, 34.4, 55 . 2, and 105 mg/L t est co n cent rati o n s, m ortali ty was 0 , 20 , 0 , 0 , and 100 %, re spectively. Abn01mal swi mmin g behavior and difficulty swi mmin g was obser ved in the 79 and 140 mg/L te s t concentratio n s , r es p ective ly. 96 -hourLC50 = 76 . 1 m g/L (95% C . I. = 13 . 1-105 m g/L) Acute Daphnid Immobili s ation Test A 48-hour ac u t e · immobili s ation t est was conducted was s tatement provided . The s tud y was co ndu cted in Jlme 20 11 acco r ding to t he OECD Guidelines f o r T esting ofCheinicals n o . 202 Daphni a sp. , Acute Immobilisation T e s t ( 1992) . Daphnia m ag na were expo s ed to the PMN m ateri al f o r 48 h o ur s unde r semi- s tatic condi t ions wit h t he te s t m aterial repleni s hed eve1y 24 h o ur s . T we n ty dap hnid s per co n cen t r atio n ( 1 0 daphnids I vessel ; 2 replicate s I te s t g r o up ) wer e ex pos ed to t he P MN mate ri al w i t h 99 . 976% purity lmder a 16-hour li g ht: 8 -ho ur dark cycle . An initi al ran ge findin g te s t was perf01med wit h 0 , 0.10 , 1.0 , 10 and 100 m g/L t est co n cent rati o n s . For the defmi t ive t est, the t emperature ran ged from 19 .7

- 20 . 0 ° C , DO= 8.4

- 8.8 m g/L , pH= 8.0

- 8.3. The n o minal te s t co n centratio n s were 0, 5, 11 , 22, 4 7, and 100 m g/L. The mea s ured co n cent rati o n s were 0 , 1.57 , 4.27 , 7.88 , 16.8 , and 51.3 m g/L all of w hi c h were 27

- 75% of nominal. The reduction of t he te s t co n centratio n s is th ought to be due to the volati li ty of the t est materi al. Due to the volati li ty of the t est materi al , te s t co n cen t r atio n s wer e poured into each of the five t est vesse l s per gr o up and immediately plugged w i t h a ground -in s to pper to preventvo latilizati o n of t he tes t s ub stan ce . An al ys i s of the te st m ateri al was p e rf o nn ed at the be g inning and end of eac h r e ne wal period . Tes t concenu·atio n s were sam pled at two rene wal set s o f f o ur durin g the 48-hour exposure period . Each te st g r o up was collect ed and analyzed b y GC/MS wit h a detectio n limit (LO D ) = 0.00005 m g/L. No m ortali ty was o b ser ved in the conu · ol g r o up . In the 1.5 7, 4 . 27, 7 . 88, 16 . 8, and 51.3 m g/L t est co n cenu ·a ti o n s, m ortali ty was 0 , 0 , 0 , 30 , and 95 %, respectively . 48-hour EC50 = 22 . 5 m g/L (95% C . I. = 17 .9

- 29.6 m g/L) Algal Toxicity Test A 72 -h o ur s tud y was sta t e ment provided . The st u dy was co n du cted in Jlme 20 11 accord ing to the OECD Guidelines f or Tes t ing o f Chemi cals n o . 20 1 Freshwater Alga and Cyanobact e ri a, Growt h Inhibiti o n Test (2006). Green algae (P seud o .kir c hneriell a s ub ca pitata) were exposed to the P MN m aterial (99.976% purity) f o r 72 h o ur s sta ti c condi t ions. The ini t ial cell de n s i ty was 5 X 103 ce ll s / mL in each of 6 vesse l s for the conu · ol and 3 vessels f or each te st co n cen t ration. The te st vessels wer e in a closed syste m and were s haken co ntinu o u s l y at 100 1pm . Test vesse l s were exposed t o co n t inuous li g h t at 61-64 iE / m 2/ s. An ini t ial ran ge finding tes t was p e rf o nn ed w i t h 0 , 50 , and 500 m g/L te st co n cenu ·a ti o n s . For the definiti ve tes t , t he nominal t est co n cenu ·a ti o n s were 0, 50, 89, 160 , 280, and 500 m g/L . The me as ured co n cen t rations wer e 0 , 1.88 , 3.00 , 6.92 , 13.4 , and 23.7 m g/L all o f w hi c h wer e 1- 35% o f n o minal. The low agree ment betwe en n o min al and mea s ured valu es for the te st co n cen t r atio n s is m ost likely due to the volati li ty o f the t est materi al. T o co mpen sate for the expected loss a nd t he vo latility of t he tes t m aterial , tes t co n cenu·atio n s were de t ennined above the m aximum upper limit co n ce nu ·a

ti o n t han w h at is suggested in the g uideline 

### ( > 100 m g/L). For the defmi t ive t est, the t emperature ranged from 2 1.0- 22 . 5 ° C , pH= 7.5

- 10 . 1. Analysis of the te st m ateri al was p e rf o nned at the beginning and end of eac h r e ne wal period . Test co n ce nu ·a

ti o n s were sa mpled at two renewal sets of four during t he 72 -ho ur expos ure period . Each tes t g r o up was co ll ected and anal yzed by GC/MS w i t h a de t ec t ion limi t (LOD ) = 0 . 00005 m g/ L. 72 -ho ur ErC50 = > 23.7 m g/L NOErC = 6.92 m g/L n ature of the P MN te st m ateri al , the A ll t hree t ests are co n sidered valid . D es pite t he studi es wer e co ndu cted in a m ann e r t o acco unt and mean , m eas ure d co n ce nu ·a

ti o n s were r ep01 ted. The mea s ured valu es '"'"'"u-.. .... .., .... we ll with ECOSAR (v. 1.1) predicted valu es and are w i t hin the same o rde r o f m ag nitude. FactorsiValues 

# I Comments

### A ss e ss ment F



### Ecotox Factors Comments:

SATChair:- Fate assessor: Ecotox assessor: Health assessor: