Exhibit 31.1

              CERTIFICATION OF CHIEF OPERATING OFFICER PURSUANT TO
                  SECTION 302 OF THE SARBANES-OXLEY ACT OF 2002

I, Roger I. Gale, certify that:

1. I have  reviewed  this  annual  report on Form  10-KSB of Calypte  Biomedical
Corporation;

2. Based on my knowledge, this report does not contain any untrue statement of a
material fact or omit to state a material fact  necessary to make the statements
made, in light of the  circumstances  under which such statements were made, not
misleading with respect to the period covered by this report;

3.  Based  on my  knowledge,  the  financial  statements,  and  other  financial
information included in this report, fairly present in all material respects the
financial condition,  results of operations and cash flows of the small business
issuer as of, and for, the periods presented in this annual report;

4. The small business issuer's other certifying officer(s) and I are responsible
for establishing and maintaining  disclosure controls and procedures (as defined
in Exchange  Act Rules  13a-15(e)  and 15d -15(e)),  and  internal  control over
financial  reporting (as defined in Exchange Act Rules  13a-15(f) and 15d-15(f))
for the small business issuer and have:

           (a) Designed such disclosure controls and procedures,  or caused such
           disclosure   controls  and   procedures  to  be  designed  under  our
           supervision,  to ensure  that  material  information  relating to the
           small business issuer,  including its consolidated  subsidiaries,  is
           made known to us by others within those entities, particularly during
           the period in which this annual report is being prepared;

           (b) Designed  such  internal  control over  financial  reporting,  or
           caused such internal control over financial  reporting to be designed
           under our supervision,  to provide reasonable assurance regarding the
           reliability of financial  reporting and the  preparation of financial
           statements  for  external   purposes  in  accordance  with  generally
           accepted accounting principles;

           (c)  Evaluated  the  effectiveness  of the  small  business  issuer's
           disclosure controls and procedures,  and presented in this report our
           conclusions  about the  effectiveness of the disclosure  controls and
           procedures,  as of the end of the period covered by this report based
           on such evaluation; and

           (d)  Disclosed  in this  report  any  change  in the  small  business
           issuer's  internal  control over  financial  reporting  that occurred
           during the small  business  issuer's most recent fiscal  quarter (the
           small  business  issuer's  fourth  fiscal  quarter  in the case of an
           annual report) that has materially affected,  or is reasonably likely
           to materially  affect,  the small business  issuer's internal control
           over financial reporting; and;

5. The small business issuer's other certifying officer(s) and I have disclosed,
based on our most recent evaluation, to the small business issuer's auditors and
the audit  committee  of the small  business  issuer's  board of  directors  (or
persons performing the equivalent function):
           (a) all  significant  deficiencies  and  material  weaknesses  in the
design or operation of internal  controls  over  financial  reporting  which are
reasonably  likely to adversely  affect the small business  issuer's  ability to
record, process, summarize and report financial information; and

           (b) any fraud,  whether or not material,  that involves management or
other  employees  who have a  significant  role in the small  business  issuer's
internal controls over financial reporting.


Date: March 31, 2006

/s/ Roger I. Gale
-----------------
Roger I. Gale
President and Chief Executive Officer