Aquatic Ecological 

Risk Assessment 

Appendices

November 1998

United States Environmental

Protection Agency

Chicago, Illinois

EVSENVIRONMENT

CONSULTANTS

Seattle, Washington 

Prepared for: Prepared by:

Sheboygan River and Harbor

Volume 3 of 3

Seattle, Washington 

M

A

O

N

NAL OCE I A C AND AT OSPHERIC ADMINISTR T OI N

I TANU.S.DEPA ENT TM R

OF COMMERCE National Oceanic and

Atmospheric Administration
Sheboygan River and Harbor

AQUATIC ECOLOGICAL RISK ASSESSMENT

Volume 3 of 3

Appendices

Prepared for

United States Environmental Protection Agency,

Region 5

77 West Jackson Blvd.

Chicago, IL 60604

Prepared by

National Oceanic and Atmospheric Administration

7600 Sand Point Way NE

Seattle, WA 98115

and

EVS Environment Consultants, Inc.

200 West Mercer Street, Suite 403

Seattle, WA 98119

EVS Project No.

2/575-37, 2/789-10

NOVEMBER 1998
TABLE OF CONTENTS

VOLUME 3 OF 3

APPENDIX A

Analytical Data

A-1 1997 Ecological Risk Assessment Results

A-2 Quality Assurance Review Summaries, Chemical Analyses of Sediment

and Fish Tissue Samples

A-3 WDNR Food Chain Study Results

APPENDIX B

Toxic Equivalent Calculations

B-1 Toxic Equivalent Concentrations for Fish

B-2 Toxic Equivalent Concentrations for Birds

B-3 Toxic Equivalent Concentrations for Mammals

APPENDIX C

Laboratory Report, Freshwater Sediment Toxicity Testing Program

APPENDIX D

Benthic Community Data

APPENDIX E

Life History of Limnodrilus hoffmeisteri and L. cervix

APPENDIX F

Parameterization of the Time-Dependent Food Web Bioaccumulation Model

APPENDIX G

Impacts on Lake Michigan Salmonids

APPENDIX H

Recommendations for Long-Term Monitoring

APPENDIX I

Analytical Methods

APPENDIX J

Taxonomic Methods
APPENDIX A

Analytical Data
APPENDIX A-1

1997 Ecological Risk Assessment Results
LIST OF TABLES

Table A1-1. Results of the metal analyses of sediment samples collected in

August 1997

Table A1-2. Results of the PCB and pesticide analyses of sediment samples

collected in August 1997

Table A1-3. Results of the PAH analyses of sediment samples collected in

August 1997

Table A1-4. Results of the conventional parameter analyses of sediment samples

collected in August 1997

Table A1-5. Results of the SEM/AVS analyses of sediment samples collected in

August 1997

Table A1-6. Results of the PCB congener analyses of sediment samples

collected in August 1997

Table A1-7. Results of the PCB congener analyses of juvenile smallmouth bass

tissue samples collected in August 1997
APPENDIX A-1

DEFINITION OF TERMS

Definition of qualifiers

> - sample over linear range of method

D - diluted sample

J - estimated value above detection limit below the quantitation limit

L - labeled compound

R - peak detected but did not meet quantification criteria

U - undetected 

Y - raised detection limit due to interference

1997 ERA sample identification 

T01 through T20 - sediment quality triad stations; FD indicates a field duplicate

S1-1

sediment sample river segment sample number

F1-1

fish tissue sample river segment sample number
Table A1-1. Results of the metal analyses of sediment samples collected in August 1997

Arsenic Cadmium Chromium Copper Lead Mercury Nickel Silver Zinc

Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

(mg/kg dw) (mg/kg dw) (mg/kg dw) (mg/kg dw) (mg/kg dw) (mg/kg dw) (mg/kg dw) (mg/kg dw) (mg/kg dw)

Station

T01 0.9 0.05 7 4.4 4 0.02 5 0.03 U 14.8

T02 1.4 0.23 18 15.1 13 0.08 11 0.05 U 46.2

T03 0.8 0.06 9 6.5 5 0.01 6 0.03 U 17.4

T04 1.9 0.25 18 16.6 16 0.08 11 0.04 U 60

T07 2.4 0.47 78.6 29.1 47 0.2 15 0.25 96.1

T08 1 0.19 12.5 11.7 12 0.04 7 0.03 U 38.8

T09 0.9 0.12 16.8 25.1 32 0.03 8 0.03 44.1

T10 2.4 0.41 33 36 36 0.08 19 0.05 U 98.2

T10-FD 2.5 0.39 32 35.3 35 0.09 18 0.05 U 96.3

T11 1.7 0.33 21.5 32 47 0.04 11 0.04 81.4

T11-FD 2.1 0.28 21.8 32 44 0.06 11 0.04 U 86.3

T12 0.8 0.13 13.4 16.3 21 0.03 8 0.03 U 39.5

T13 1.9 0.7 28.4 29 128 0.07 14 0.05 94.4

T14 1.9 0.46 26 29.6 49 0.06 12 0.05 93.2

T15 2.1 0.42 28 34.2 45 0.08 16 0.05 99

T16 2.2 0.48 30 35.4 49 0.08 18 0.06 111

T17 1.3 0.21 16.5 18.6 23 0.04 9 0.03 52.5

T18 2 0.36 27 30.9 34 0.07 15 0.05 89.9

T19 2 0.34 25 28.3 48 0.07 15 0.04 U 87.2

T20 2.8 0.47 36 37 37 0.1 19 0.06 U 112
Table A1-2. Results of the PCB and pesticide analyses of sediment samples collected in August 1997

Chlordane - alpha Chlordane - gamma gamma-HCH (Lindane) Dibenzofuran Dieldrin Endrin

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

T01 1 U 1 U 1 U 5.8 U 1.9 U 1.9 U 

T02 1 U 1 U 1 U 5.8 U 1.9 U 1.9 U 

T03 1 U 1 U 1 U 5.9 U 2 U 2 U 

T04 1 U 1 U 1 U 6 U 2 U 2 U 

T07 310 Y 1000 Y 81 U 5.8 U 160 U 160 U 

T08 4.9 Y 31 Y 0.9 U 5.7 U 12 Y 7 Y 

T09 0.8 Y 7.1 Y 0.7 U 12 4.8 Y 2.3 Y 

T10 1 U 5.8 Y 1 U 5.8 U 3 Y 1.9 U 

T10-FD 1 U 6 Y 1 U 6 U 3.6 Y 2 U 

T11 1 U 5.9 Y 1 U 26 2.6 Y 1.9 U 

T11-FD 0.9 U 5.5 Y 0.9 U 27 3.3 Y 1.9 U 

T12 1 U 5.3 Y 1 U 18 2.4 Y 1.9 U 

T13 1 U 9 Y 1 U 18 5.2 Y 3 Y 

T14 1 U 9.6 Y 1 U 24 5.8 2.7 Y 

T15 1 U 8.6 Y 1 U 12 5 Y 2.6 Y 

T16 1 U 9.9 Y 1 U 15 5.4 Y 2.8 Y 

T17 1 U 10 Y 1 U 10 5.8 Y 3 Y 

T18 1 U 9.5 Y 1 U 8.9 5.4 Y 3.2 Y 

T19 1 U 9.3 Y 1 U 31 5.2 Y 2.6 Y 

T20 1 U 8.7 Y 1 U 8.3 4.2 Y 2.1 Y 

All concentrations are in ug/kg, dry weight.

HCH - Hexachlorocyclohexane-gamma (Lindane)
Table A1-2. Results of the PCB and pesticide analyses of sediment samples collected in August 1997

Heptachlor epoxide Aroclor 1016 Aroclor 1221 Aroclor 1232 Aroclor 1242 Aroclor 1248

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

T01 1 U 19 U 39 U 19 U 19 U 19 U 

T02 1 U 19 U 39 U 19 U 19 U 19 U 

T03 1 U 19 U 39 U 19 U 19 U 19 U 

T04 1 U 20 U 40 U 20 U 20 U 20 U 

T07 81 U 1600 U 3300 U 750000 D 1600 U 1600 U 

T08 21 Y 19 U 38 U 19 U 920 19 U 

T09 5.5 Y 15 U 30 U 15 U 78 15 U 

T10 4.9 Y 19 U 38 U 19 U 71 19 U 

T10-FD 5.2 Y 20 U 40 U 20 U 80 20 U 

T11 4 Y 19 U 39 U 19 U 69 19 U 

T11-FD 4.4 Y 19 U 38 U 19 U 84 19 U 

T12 4.5 Y 19 U 38 U 19 U 71 19 U 

T13 7.1 Y 19 U 39 U 19 U 140 19 U 

T14 7.8 Y 19 U 38 U 19 U 150 19 U 

T15 6.6 Y 20 U 39 U 20 U 120 20 U 

T16 7.8 Y 19 U 39 U 19 U 150 19 U 

T17 7.5 Y 19 U 38 U 19 U 150 19 U 

T18 7.4 Y 20 U 39 U 20 U 120 20 U 

T19 7.3 Y 19 U 39 U 19 U 170 19 U 

T20 7.5 Y 20 U 40 U 20 U 150 20 U 

All concentrations are in ug/kg, dry weight.
Table A1-2. Results of the PCB and pesticide analyses of sediment samples collected in August 1997

Aroclor 1254 Aroclor 1260 p,p'-DDD p,p'-DDE p,p'-DDT

Station Conc Q Conc Q Conc Q Conc Q Conc Q

T01 19 U 19 U 1.9 U 1.9 U 1.9 U 

T02 19 U 19 U 1.9 U 1.9 U 1.9 U 

T03 19 U 19 U 2 U 2 U 2 U 

T04 20 U 20 U 2 U 2 U 2 U 

T07 8100 Y 1600 U 160 U 710 Y 240 Y 

T08 1000 19 U 2.6 Y 16 Y 6.6 Y 

T09 220 15 U 1.5 U 4.6 Y 3.7 Y 

T10 150 19 U 1.9 U 3.7 Y 2.9 Y 

T10-FD 160 20 U 2 U 3.9 Y 3 Y 

T11 120 19 U 2.1 Y 3.5 Y 2.3 Y 

T11-FD 160 19 U 1.9 U 3.6 Y 2.8 Y 

T12 110 19 U 1.9 U 4 Y 2.8 Y 

T13 270 19 U 2.7 Y 6.5 Y 4.8 Y 

T14 290 19 U 2.5 Y 7.3 4.8 Y 

T15 240 20 U 2 U 6.2 Y 3.9 Y 

T16 270 19 U 2.2 Y 7.1 Y 3.9 Y 

T17 260 19 U 1.9 U 6.4 Y 3.8 Y 

T18 250 20 U 2 U 6.4 Y 3.9 Y 

T19 270 19 U 1.9 U 5.8 Y 3.3 Y 

T20 200 20 U 2 U 6 Y 3 Y 

All concentrations are in ug/kg, dry weight.
Table A1-3. Results of the PAH analyses of sediment samples collected in August 1997

Acenaphthene Acenaphthylene Anthracene Benz(a)anthracene Dibenz(a,h)anthracene Benzo(a)pyrene

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

T01 5.8 U 5.8 U 5.8 U 16 5.8 U 26

T02 5.8 U 5.8 U 5.8 U 12 J 5.8 U 16

T03 5.9 U 5.9 U 5.9 U 5.9 U 5.9 U 5.9 J 

T04 6 U 6 U 10 60 10 68

T07 5.8 U 5.8 U 5.8 U 29 5.8 U 34

T08 7.4 J 5.7 U 22 78 15 91

T09 15 4.4 U 43 110 5.8 110

T10 5.8 U 5.8 U 16 80 6.9 100

T10-FD 6 U 6 U 12 71 7.2 92

T11 44 7.5 120 490 34 490

T11-FD 40 5.7 U 93 430 82 460

T12 29 5.7 U 80 300 36 290

T13 24 6.4 110 440 21 390

T14 31 8 60 310 17 320

T15 18 16 57 260 26 300

T16 24 12 40 240 16 250

T17 25 5.7 U 55 170 24 180

T18 13 5.9 U 30 150 17 190

T19 49 32 110 580 75 680

T20 11 5.9 U 26 150 31 180

All concentrations are in ug/kg, dry weight.

Note: Sum of PAHs does not include Dibenzofuran and Carbazole
Table A1-3. Results of the PAH analyses of sediment samples collected in August 1997

Benzo(b)fluoranthene Benzo(g,h,i)perylene Benzo(k)fluoranthene Carbazole Chrysene

Station Conc Q Conc Q Conc Q Conc Q Conc Q

T01 22 14 J 21 7.5 J 27

T02 17 5.8 U 11 J 5.8 U 14 J 

T03 5.9 J 7 J 5.9 J 5.9 U 6.4 J 

T04 80 30 64 13 72

T07 47 18 31 5.8 U 44

T08 83 42 91 12 94

T09 160 35 78 25 95

T10 160 40 87 17 99

T10-FD 120 57 89 17 99

T11 550 250 380 92 470

T11-FD 480 240 410 92 460

T12 350 90 180 51 240

T13 420 140 410 60 410

T14 440 120 270 64 350

T15 350 190 290 39 330

T16 320 100 230 36 270

T17 180 90 160 25 200

T18 200 130 180 27 190

T19 670 260 490 68 550

T20 200 98 170 30 190

All concentrations are in ug/kg, dry weight.

Note: Sum of PAHs does not include Dibenzofuran and Carbazole
Table A1-3. Results of the PAH analyses of sediment samples collected in August 1997

Fluoranthene Fluorene Indeno(1,2,3-c,d)pyrene 2-Methylnaphthalene Naphthalene

Station Conc Q Conc Q Conc Q Conc Q Conc Q

T01 55 5.8 U 16 5.8 U 5.8 U 

T02 27 5.8 U 10 J 5.8 U 5.8 U 

T03 11 J 5.9 U 9.4 J 5.9 U 5.9 U 

T04 160 8.4 43 6 U 6 U 

T07 63 6.9 J 18 9.2 J 9.2 J 

T08 160 13 J 53 5.7 U 5.7 U 

T09 240 21 53 7.1 6.2

T10 180 9.8 69 14 9.8

T10-FD 230 11 75 14 11

T11 1600 68 340 9.8 12

T11-FD 1100 64 320 19 17

T12 500 39 140 12 16

T13 1300 49 200 18 18

T14 860 47 170 24 27

T15 800 33 230 17 17

T16 560 31 150 28 24

T17 420 36 110 17 21

T18 380 19 150 9.4 10

T19 1200 69 370 41 36

T20 440 18 130 8.9 9.5

All concentrations are in ug/kg, dry weight.

Note: Sum of PAHs does not include Dibenzofuran and Carbazole
Table A1-3. Results of the PAH analyses of sediment samples collected in August 1997

Phenanthrene Pyrene PAHs, total

Station Conc Q Conc Q Conc Q

T01 57 74 348.3

T02 12 J 21 163.2

T03 8.2 J 12 J 95.3

T04 84 120 821.4

T07 48 68 436.9

T08 120 220 1097.95

T09 190 170 1341.3

T10 100 160 1137.3

T10-FD 110 140 1144.2

T11 770 860 6495.3

T11-FD 700 1200 6117.85

T12 360 410 3074.85

T13 500 680 5136.4

T14 440 600 4094

T15 340 500 3774

T16 260 430 2985

T17 300 320 2310.85

T18 200 400 2271.35

T19 580 1400 7192

T20 210 340 2215.35

All concentrations are in ug/kg, dry weight.

Note: Sum of PAHs does not include Dibenzofuran and Carbazole
Table A1-4. Results of the conventional parameter analyses of sediment samples collected in August 1997

Fines Gravel Sand Total solids Total organic carbon

Station (%) (%) (%) (%) (%)

T01 12 5 83 63.8 5.2

T02 48 19 33 34.2 8.3

T03 20 9 71 69.8 4.9

T04 61 3 36 36.6 5.9

T07 60 2 38 50.3 6.3

T08 35 7 58 47.7 5.8

T09 26 1 73 64.3 4.6

T10 90 0 10 35.4 4.4

T10-FD 90 0 10 34.6 5.2

T11 45 5 50 45.9 5.2

T11-FD 44 4 52 44.6 4.6

T12 16 1 83 74 4.8

T13 62 4 34 47 5.6

T14 65 0 35 48.2 5

T15 79 0 21 40.6 5.5

T16 80 1 19 39.5 5.6

T17 30 3 67 58.8 5.1

T18 68 1 31 43.9 5.4

T19 58 4 38 45.2 5.4

T20 87 0 13 33.5 6.1

S1-1 8 6 86 77.9 5.1

S1-2 61 19 20 28.8 6.2

S1-3 24 6 70 57.4 5.2

S2-1 19 5 76 60.2 5.7

S2-2 54 6 40 56.3 6.4

S2-3 44 3 53 48 5.9

S3-1 8 1 91 76.1 4.7

S3-2 66 8 26 43.9 5.4

S3-3 40 6 54 42.8 5.8

S5-1 25 5 70 69.4 4.4

S5-2 9 1 90 77.6 3.4

S5-3 24 3 73 59.3 4.5

S5-4 31 1 68 63.6 5.1

S5-5 14 38 48 65.9 5.7
Table A1-5. Results of the SEM/AVS analyses of sediment samples collected in August 1997

SEM/AVS Acid Volatile Sulfides Cadmium Copper Lead Mercury

(umol/g) (mg/kg) (mg/kg) (mg/kg) (mg/kg) (mg/kg)

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

T01 0.18517 39 0.4 U 2.3 4 U 0.004 U 

T02 0.12893 200 0.2 U 11.2 9 0.01 U 

T03 0.0797 130 0.3 U 5.3 4 0.006 U 

T04 0.28953 110 0.4 U 13.3 12 0.009 U 

T07 0.10075 530 0.4 U 19.7 40 0.009 U 

T08 0.04908 450 0.3 U 8.9 9 0.008 U 

T09 0.76609 42 0.3 U 16.2 32 0.007 U 

T10 0.29186 180 0.5 U 24.5 31 0.01 U 

T10-FD 0.22447 230 0.5 U 23.3 30 0.01 U 

T11 0.35648 130 0.3 U 17.3 36 0.008 U 

T11-FD 0.29502 130 0.3 U 14.3 29 0.007 U 

T12 0.66978 40 0.3 U 10.5 19 0.003 U 

T13 0.49856 110 0.4 20.3 61 0.006 U 

T14 0.33418 160 0.4 U 20.2 43 0.01 U 

T15 0.74732 78 0.4 U 24.2 36 0.01 U 

T16 0.37578 170 0.4 25.6 39 0.01 U 

T17 0.35134 100 0.2 U 15.2 26 0.005 U 

T18 0.6645 88 0.3 23.5 32 0.01 U 

T19 1.20699 41 0.4 U 20.9 32 0.01 U 

T20 1.66342 40 0.4 28.8 33 0.01 U 

Note: SEM/AVS was calculated as the sum of SEMs/AVS and does not include SEM mercury
Table A1-5. Results of the SEM/AVS analyses of sediment samples collected in August 1997

Nickel Zinc SEM Cadmium SEM Copper SEM Lead

(mg/kg) (mg/kg) (umol/g) (umol/g) (umol/g)

Station Conc Q Conc Q Conc Q Conc Q Conc Q

T01 2 U 10.5 0.00356 U 0.03619 0.01931 U 

T02 5 32.6 0.00178 U 0.17624 0.04344

T03 2 12.1 0.00267 U 0.0834 0.01931

T04 5 41.8 0.00356 U 0.20928 0.05792

T07 7 68.1 0.00356 U 0.30999 0.19305

T08 4 28.5 0.00267 U 0.14005 0.04344

T09 5 33.2 0.00267 U 0.25492 0.15444

T10 8 63.1 0.00445 U 0.38552 0.14961

T10-FD 8 62.8 0.00445 U 0.36664 0.14479

T11 5 59.7 0.00267 U 0.27223 0.17375

T11-FD 5 48.7 0.00267 U 0.22502 0.13996

T12 4 33.3 0.00267 U 0.16522 0.0917

T13 6 64.8 0.00356 0.31943 0.2944

T14 6 67.9 0.00356 U 0.31786 0.20753

T15 8 73.6 0.00356 U 0.3808 0.17375

T16 8 82.5 0.00356 0.40283 0.18822

T17 4 43.3 0.00178 U 0.23918 0.12548

T18 8 75.9 0.00267 0.36979 0.15444

T19 8 60.3 0.00356 U 0.32887 0.15444

T20 9 85.4 0.00356 0.45319 0.15927

Note: SEM/AVS was calculated as the sum of SEMs/AVS and does not include SEM mercury
Table A1-5. Results of the SEM/AVS analyses of sediment samples collected in August 1997

SEM Nickel SEM Zinc SEM Sum

(umol/g) (umol/g) (umol/g)

Station Conc Q Conc Q Conc Q

T01 0.03408 U 0.16058 0.22525

T02 0.08519 0.49855 0.80431

T03 0.03408 0.18504 0.32317

T04 0.08519 0.63924 0.99341

T07 0.11927 1.04144 1.66553

T08 0.06815 0.43585 0.68883

T09 0.08519 0.50772 1.00361

T10 0.13631 0.96498 1.63865

T10-FD 0.13631 0.96039 1.61036

T11 0.08519 0.91298 1.44549

T11-FD 0.08519 0.74476 1.19627

T12 0.06815 0.50925 0.83566

T13 0.10223 0.99098 1.7106

T14 0.10223 1.03839 1.66779

T15 0.13631 1.12555 1.81819

T16 0.13631 1.26166 1.99258

T17 0.06815 0.66218 1.09588

T18 0.13631 1.16073 1.82394

T19 0.13631 0.92216 1.54356

T20 0.15335 1.30601 2.07538

Note: SEM/AVS was calculated as the sum of SEMs/AVS and does not include SEM mercury
Table A1-6. Results of the PCB congener analyses of sediment samples collected in August 1997

PCB008/005 PCB015 PCB016/032 PCB017 PCB018 PCB019

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

S1-1 0.12 R 0.75 R 0.14 0.16 0.08 U 0.08 U 

S1-2 0.11 2.7 R 0.16 R 0.1 0.07 U 0.07 U 

S1-3 0.06 U 3.7 R 0.12 R 0.04 U 0.04 U 0.04 U 

S2-1 64 180 120 170 98 40

S2-2 260 550 670 770 130 250

S2-3 130 14 R 150 210 81 65

S3-1 130 13 R 180 210 33 90

S3-2 8000 1000 4900 5500 150 3900

S3-3 66 10 75 130 35 23

S5-1 19 26 35 32 20 12

S5-2 3 1.1 U 6.3 7 5 1.5

S5-3 19 3.1 29 38 19 5.7

S5-4 10 1.8 R 12 20 7.9 3

S5-5 13 24 R 14 21 9.9 5.9

All concentrations are in ug/kg, dry weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-6. Results of the PCB congener analyses of sediment samples collected in August 1997

PCB022 PCB024/027 PCB025 PCB026 PCB031/028 PCB033

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

S1-1 0.05 U 0.08 U 0.05 U 0.05 U 0.28 0.05 U 

S1-2 0.05 U 0.07 U 0.05 U 0.05 U 0.31 0.05

S1-3 0.03 R 0.06 R 0.03 U 0.03 U 0.03 U 0.03 U 

S2-1 22 53 83 100 260 36

S2-2 120 200 180 200 1000 76

S2-3 21 75 79 100 290 30

S3-1 9.5 120 44 61 140 12

S3-2 36 2800 86 480 490 58

S3-3 7 47 46 59 170 13

S5-1 9.1 16 17 24 120 8.4

S5-2 1.9 3.3 5.7 8.9 34 2.6

S5-3 4.5 15 22 30 160 6.2

S5-4 2.1 6.9 10 14 67 3.2

S5-5 2.6 8.4 9.4 R 12 47 3.3

All concentrations are in ug/kg, dry weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-6. Results of the PCB congener analyses of sediment samples collected in August 1997

PCB040 PCB041/071/006 PCB042 PCB044 PCB045 PCB046

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

S1-1 0.03 U 0.2 0.05 0.13 0.05 U 0.01 U 

S1-2 0.07 U 0.46 0.13 0.47 0.05 U 0.05 U 

S1-3 0.03 U 0.03 U 0.03 U 0.03 U 0.02 U 0.02 U 

S2-1 5.2 200 83 160 25 7.1

S2-2 31 1200 360 740 100 29

S2-3 7.6 210 84 140 25 7.2

S3-1 4.2 140 73 73 13 6.9

S3-2 18 630 280 190 68 45

S3-3 5.7 110 48 77 10 2.1

S5-1 6.6 R 130 43 84 11 4.3

S5-2 1.1 U 24 9.2 18 2.3 0.93 U 

S5-3 3.4 86 30 66 5 0.28 R 

S5-4 1.4 37 13 27 2.1 0.5

S5-5 0.82 27 11 18 2.3 0.71

All concentrations are in ug/kg, dry weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-6. Results of the PCB congener analyses of sediment samples collected in August 1997

PCB047/048 PCB049 PCB052 PCB056/060 PCB066 PCB070/076

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

S1-1 0.13 0.14 0.18 0.08 0.15 0.16

S1-2 0.23 0.37 0.69 0.27 0.36 0.92

S1-3 0.03 U 0.03 U 0.08 0.02 U 0.06 R 0.11

S2-1 160 200 220 50 110 170

S2-2 860 810 1100 420 720 780

S2-3 170 190 240 49 100 160

S3-1 180 160 170 22 54 62

S3-2 1900 560 760 74 140 170

S3-3 140 140 160 27 75 100

S5-1 82 95 120 55 90 140

S5-2 16 24 32 8.1 17 28

S5-3 88 100 120 30 87 140

S5-4 40 44 52 13 39 58

S5-5 25 28 31 9 17 26

All concentrations are in ug/kg, dry weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-6. Results of the PCB congener analyses of sediment samples collected in August 1997

PCB074 PCB077* PCB083 PCB084/089 PCB085 PCB087

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

S1-1 0.12 0.00398 0.03 U 0.08 0.08 0.13

S1-2 0.3 0.0363 0.08 U 0.48 0.31 0.64

S1-3 0.03 U 0.00871 0.02 U 0.02 U 0.02 U 0.12

S2-1 120 6.84 9.4 52 20 42

S2-2 750 68.2 D 65 300 230 490

S2-3 110 9.12 13 R 72 27 62

S3-1 57 7.53 15 R 81 16 45

S3-2 130 11.3 33 220 53 240

S3-3 77 8.33 11 44 22 50

S5-1 89 10.9 11 41 31 64

S5-2 18 2.1 2.9 7.3 6.6 12

S5-3 92 8.62 7.2 26 24 49

S5-4 39 6.32 3.6 13 12 23

S5-5 19 1.82 D 1.6 8 4.4 8.6

All concentrations are in ug/kg, dry weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-6. Results of the PCB congener analyses of sediment samples collected in August 1997

PCB090/101 PCB091 PCB095 PCB097 PCB099 PCB105

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

S1-1 0.24 0.03 0.12 0.1 0.12 0.13

S1-2 1.4 0.16 0.86 0.46 0.6 0.55

S1-3 0.23 0.03 R 0.12 0.02 U 0.09 0.1

S2-1 92 28 94 40 54 32

S2-2 700 180 580 330 420 370

S2-3 130 40 120 49 74 38

S3-1 91 53 94 26 45 22

S3-2 270 490 320 64 120 67

S3-3 91 31 84 39 56 33

S5-1 90 22 71 46 50 46

S5-2 17 4.4 16 9.3 10 8.8

S5-3 78 19 65 35 49 40

S5-4 38 8.9 31 17 22 17

S5-5 17 4.5 14 6.8 9.9 7.2

All concentrations are in ug/kg, dry weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-6. Results of the PCB congener analyses of sediment samples collected in August 1997

PCB107 PCB110 PCB114* PCB118 PCB123* PCB126*

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

S1-1 0.02 0.4 0.00244 L 0.24 0.00109 L 0.00051 U 

S1-2 0.11 2.1 0.0208 L 1.3 0.011 0.00714 L 

S1-3 0.01 U 0.38 0.00462 L 0.24 0.00496 L 0.0005 U 

S2-1 6.4 180 2.4 76 0.966 0.115

S2-2 47 1300 23 710 10.7 1.76

S2-3 10 240 3.17 99 1.42 0.203

S3-1 6.6 180 2.93 56 1.2 0.177

S3-2 19 R 660 5.87 120 1.71 0.34

S3-3 7.5 170 3.73 73 1.54 0.229

S5-1 7.5 R 180 4.99 81 2.18 0.299

S5-2 1.8 34 0.954 15 0.344 0.0619

S5-3 5.9 130 4.16 73 1.81 0.276

S5-4 3.1 62 2.65 34 1.12 0.194

S5-5 1.2 33 0.607 15 0.239 0.0479

All concentrations are in ug/kg, dry weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-6. Results of the PCB congener analyses of sediment samples collected in August 1997

PCB128 PCB129 PCB130 PCB131 PCB134 PCB136

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

S1-1 0.1 U 0.06 U 0.06 U 0.05 U 0.05 U 0.05 U 

S1-2 0.54 0.07 0.05 U 0.06 U 0.06 U 0.19

S1-3 0.06 R 0.03 U 0.03 U 0.03 U 0.03 U 0.03 U 

S2-1 9.3 2 0.05 U 0.63 4.4 11

S2-2 130 33 8.4 U 8.2 31 84

S2-3 17 3.5 0.42 U 1.2 6.3 18

S3-1 9.8 2.2 0.26 U 0.48 9.2 25

S3-2 25 R 12 U 12 U 13 U 58 200

S3-3 12 3.4 1.1 U 1.5 6.6 17

S5-1 19 7.9 U 7.9 U 9.5 U 9.5 U 13

S5-2 2.1 U 1.2 U 1.2 U 2 U 2 U 2.8

S5-3 7.6 2.5 0.21 U 0.78 R 2.8 9

S5-4 4.2 1.2 0.1 U 0.43 1.6 5

S5-5 2.5 0.5 0.03 U 0.16 R 0.72 1.8

All concentrations are in ug/kg, dry weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-6. Results of the PCB congener analyses of sediment samples collected in August 1997

PCB137 PCB138/163/164 PCB141 PCB144/135 PCB146 PCB149

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

S1-1 0.06 U 0.25 0.06 U 0.05 U 0.04 U 0.09

S1-2 0.11 1.8 0.2 0.22 0.18 0.98

S1-3 0.03 U 0.31 0.04 R 0.03 U 0.03 U 0.18

S2-1 2.4 44 4.7 8.8 5.5 40

S2-2 38 510 78 73 45 310

S2-3 5 83 8.5 17 11 69

S3-1 2.8 77 4.6 23 13 84

S3-2 12 U 480 19 R 210 69 750

S3-3 4.4 71 8.1 15 9.6 66

S5-1 7.9 U 60 11 11 6.9 U 51

S5-2 1.2 U 6.5 1.3 U 2.1 1.4 U 8.1

S5-3 3 52 6.5 8.6 5.2 40

S5-4 1.6 27 3.5 4.6 2.9 21

S5-5 0.57 11 1.1 1.7 1.2 8.1

All concentrations are in ug/kg, dry weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-6. Results of the PCB congener analyses of sediment samples collected in August 1997

PCB151 PCB153 PCB156* PCB156/157* PCB157* PCB158

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

S1-1 0.05 U 0.16 0.0115 L 0.0174 L 0.00237 L 0.06 U 

S1-2 0.21 1.2 0.134 0.188 0.0289 0.33

S1-3 0.03 U 0.22 0.026 0.0376 L 0.00593 L 0.03 U 

S2-1 8.5 27 3.36 4.79 0.663 5.7

S2-2 60 300 39.2 67.2 7.89 69

S2-3 15 52 6.42 9.43 1.31 10

S3-1 23 37 5.04 8.33 1.11 5.9

S3-2 180 140 13.8 17.2 2.48 17

S3-3 14 45 8.18 10.9 1.81 8.3

S5-1 9.5 U 33 7.53 9.9 1.54 8.3 R 

S5-2 2 U 3.8 1.43 1.83 0.299 1.2 U 

S5-3 8.4 29 6.95 9.59 1.55 6.6

S5-4 4.2 16 4.71 5.97 0.954 3.2

S5-5 1.6 6.3 1.12 1.6 0.236 1.6

All concentrations are in ug/kg, dry weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-6. Results of the PCB congener analyses of sediment samples collected in August 1997

PCB167* PCB169* PCB170* PCB170/190 PCB171 PCB172

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

S1-1 0.00599 L 0.001 U 0.018 L 0.04 U 0.04 U 0.04 U 

S1-2 0.059 0.001 U 0.288 0.24 0.06 0.02 U 

S1-3 0.0109 L 0.001 U 0.0483 0.06 R 0.02 U 0.02 U 

S2-1 1.27 0.00147 U 4.1 5.3 1 0.59

S2-2 13 0.00735 U 43 67 14 7.9 U 

S2-3 2.38 0.0022 U 8.41 9.1 1.8 1.1

S3-1 1.82 0.00163 U 7.71 9.2 1.7 1.2

S3-2 4.68 0.0028 U 31.3 44 13 U 13 U 

S3-3 2.7 0.00125 U 8.67 9.2 2.5 1.6 U 

S5-1 2.34 0.001 U 6.66 11 4.6 U 4.4 U 

S5-2 0.46 0.001 U 1.02 0.74 0.44 U 0.41 U 

S5-3 2.29 0.001 U 6.52 6.4 1.4 0.84

S5-4 1.4 0.001 U 4.1 3.5 0.77 0.46

S5-5 0.42 0.001 U 1.28 1.3 0.23 0.13

All concentrations are in ug/kg, dry weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-6. Results of the PCB congener analyses of sediment samples collected in August 1997

PCB174 PCB175 PCB176 PCB177 PCB178 PCB179

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

S1-1 0.04 U 0.03 U 0.03 U 0.04 U 0.03 U 0.03 U 

S1-2 0.17 0.02 U 0.04 R 0.09 0.03 0.1 R 

S1-3 0.04 R 0.02 U 0.02 U 0.02 U 0.02 U 0.02 U 

S2-1 2.7 0.14 0.65 2.3 0.82 1.8

S2-2 35 7.4 U 7.8 27 9.1 R 18

S2-3 4.9 0.2 U 1.4 4.5 1.9 3.8

S3-1 3.9 0.23 R 1.6 5.9 2.4 5.1

S3-2 19 12 U 18 71 25 61

S3-3 5.8 1.5 U 1.7 4.5 1.7 4

S5-1 4.8 U 4.1 U 4.1 U 4.8 U 4.1 U 4.1 U 

S5-2 0.44 U 0.38 U 0.38 U 0.44 U 0.38 U 0.38 U 

S5-3 3.7 0.22 U 0.68 2.7 0.85 2.1

S5-4 2.1 0.12 R 0.47 1.4 0.47 1.2

S5-5 0.63 0.05 U 0.14 0.48 0.19 0.4

All concentrations are in ug/kg, dry weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-6. Results of the PCB congener analyses of sediment samples collected in August 1997

PCB180* PCB183 PCB185 PCB187/182 PCB189* PCB191

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

S1-1 0.0316 0.04 U 0.04 U 0.03 U 0.001 U 0.04 U 

S1-2 0.367 0.11 0.02 U 0.23 0.001 U 0.02 U 

S1-3 0.0707 0.02 U 0.02 U 0.04 R 0.001 U 0.02 U 

S2-1 6.28 1.7 0.26 3.7 0.182 0.16

S2-2 62.2 27 8.7 U 34 1.9 7.9 U 

S2-3 11.9 3.4 0.64 7.4 0.446 0.4 R 

S3-1 12.2 2.9 0.43 9.1 0.364 0.44

S3-2 51.2 15 R 14 U 100 2.49 13 U 

S3-3 13.2 3.2 1.7 U 7.7 0.436 1.6 U 

S5-1 10.6 4.8 U 4.8 U 5.1 0.316 4.4 U 

S5-2 1.63 0.44 U 0.44 U 0.38 U 0.0458 0.41 U 

S5-3 9.67 2.2 0.54 4.2 0.324 0.25 R 

S5-4 6.38 1.4 0.26 2.5 0.232 0.12

S5-5 1.88 0.36 0.06 0.81 0.0648 0.05 U 

All concentrations are in ug/kg, dry weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-6. Results of the PCB congener analyses of sediment samples collected in August 1997

PCB193 PCB194 PCB195 PCB196/203 PCB197 PCB198

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

S1-1 0.04 U 0.1 U 0.08 U 0.08 U 0.09 R 0.08 U 

S1-2 0.02 U 0.12 R 0.06 R 0.17 0.05 U 0.05 U 

S1-3 0.02 U 0.1 U 0.08 U 0.08 U 0.09 U 0.09 U 

S2-1 0.45 1.7 0.47 1.3 0.09 U 0.09 U 

S2-2 7.9 U 20 7.7 16 8 U 8 U 

S2-3 0.77 2.4 0.63 2.3 0.29 U 0.29 U 

S3-1 0.67 2.6 0.75 2.2 0.35 R 0.17 U 

S3-2 13 U 36 U 30 U 30 U 32 U 32 U 

S3-3 1.6 U 4.8 U 3.4 U 3.4 U 3.4 U 3.4 U 

S5-1 4.4 U 11 U 9.2 U 9.2 U 9.9 U 9.9 U 

S5-2 0.41 U 0.68 U 0.59 U 0.59 U 0.61 U 0.61 U 

S5-3 0.47 R 1.8 0.6 1.5 0.39 U 0.39 U 

S5-4 0.29 1.1 0.25 0.95 0.2 U 0.2 U 

S5-5 0.08 0.43 0.15 0.37 0.04 U 0.04 U 

All concentrations are in ug/kg, dry weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-6. Results of the PCB congener analyses of sediment samples collected in August 1997

PCB199 PCB201 PCB205 PCB206 PCB207 PCB208

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

S1-1 0.08 U 0.04 U 0.1 U 0.14 U 0.14 U 0.14 U 

S1-2 0.17 0.07 0.07 U 0.15 U 0.15 U 0.15 U 

S1-3 0.09 U 0.04 U 0.1 U 0.36 U 0.36 U 0.36 U 

S2-1 1.3 0.11 0.1 0.28 0.14 U 0.14 U 

S2-2 13 3.3 U 9.1 U 14 U 14 U 14 U 

S2-3 2.4 0.13 U 0.43 U 0.65 0.49 U 0.49 U 

S3-1 2 0.24 R 0.29 R 0.63 0.24 U 0.48

S3-2 32 U 13 U 36 U 32 U 32 U 32 U 

S3-3 3.4 U 1.6 U 4.8 U 4.4 U 4.4 U 4.4 U 

S5-1 9.9 U 4 U 11 U 9.2 U 9.2 U 9.2 U 

S5-2 0.61 U 0.28 U 0.68 U 0.62 U 0.62 U 0.62 U 

S5-3 1.5 0.18 U 0.55 U 0.41 0.36 U 0.36 U 

S5-4 0.89 0.09 U 0.28 U 0.35 0.18 0.1

S5-5 0.37 0.04 R 0.05 U 1.7 0.12 U 0.87 R 

All concentrations are in ug/kg, dry weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-6. Results of the PCB congener analyses of sediment samples collected in August 1997

PCB209 PCB Sum Percent Moisture

Station Conc Q Conc Q Conc Q

S1-1 0.14 U 6.61 26

S1-2 0.16 U 26.26 66

S1-3 1.4 U 8.8 35

S2-1 0.09 U 3694.13 40

S2-2 9.5 U 20494.9 49

S2-3 0.71 U 4133.73 47

S3-1 0.3 U 3208.39 24

S3-2 13 U 37922.87 49

S3-3 2.6 U 2772.78 56

S5-1 4.5 U 2417.91 23

S5-2 0.37 U 470.59 17

S5-3 0.32 U 2067.65 36

S5-4 0.15 U 958.35 34

S5-5 0.12 U 573.69 26

All concentrations are in ug/kg, dry weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-7. Results of the PCB congener analyses of juvenile smallmouth bass

tissue samples collected in August 1997

Percent Lipids Percent Moisture PCB008/005 PCB015 PCB016/032

Station Conc Q Conc Q Conc Q Conc Q Conc Q

F1-1 3.7 75 0.54 0.22 U 0.18

F1-2 3.5 75 0.37 0.22 U 0.1

F1-3 3.1 75 0.46 0.29 U 0.13

F2-1 3.7 D 75 D 120 >D 44 D 200 >D 

F2-2 2.9 D 75 D 78 D 31 D 140 D 

F2-3 3.1 D 75 D 130 >D 39 D 170 >D 

F3-1 2.5 76 200 62 260

F3-2 2 76 140 42 210

F3-3 2.4 76 160 54 230

F5-1 2.7 76 94 42 96

F5-2 2.5 76 90 35 89

F5-3 2.8 76 140 51 140

All concentrations are in ug/kg, wet weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.

U - Undetected

R - Peak detected but did not meet quantification criteria

D - Diluted sample

L - Labelled compound

> - Sample over linear range of method

Note: No qualifier for the PCB_SUM values indicates that at least one value was detected in either sediment or tissue.
Table A1-7. Results of the PCB congener analyses of juvenile smallmouth bass

tissue samples collected in August 1997

PCB017 PCB018 PCB019 PCB022 PCB024/027 PCB025

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

F1-1 0.33 0.09 0.11 0.05 0.12 0.08

F1-2 0.15 0.06 0.06 0.04 0.05 0.05

F1-3 0.17 0.05 0.07 R 0.03 0.06 0.05

F2-1 300 >D 81 D 71 D 31 D 97 D 91 D 

F2-2 230 D 84 D 38 D 31 D 66 D 97 D 

F2-3 240 >D 64 D 72 D 27 D 82 D 82 D 

F3-1 430 85 100 32 150 130

F3-2 310 62 79 30 110 100

F3-3 330 70 90 30 130 110

F5-1 210 48 61 17 87 61

F5-2 200 43 59 16 82 55

F5-3 330 71 94 21 140 84

All concentrations are in ug/kg, wet weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-7. Results of the PCB congener analyses of juvenile smallmouth bass

tissue samples collected in August 1997

PCB026 PCB031/028 PCB033 PCB040 PCB041/071/006 PCB042

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

F1-1 0.11 0.66 0.06 0.05 R 0.87 0.27

F1-2 0.05 0.45 0.05 0.05 R 0.7 0.22

F1-3 0.06 0.41 0.04 R 0.04 U 0.64 0.22

F2-1 110 D 480 >D 42 D 21 D 700 >D 250 >D 

F2-2 130 D 660 >D 47 D 16 D 750 >D 270 D 

F2-3 100 D 450 >D 29 D 11 D 420 >D 150 D 

F3-1 150 660 38 17 760 280

F3-2 120 540 29 19 660 250

F3-3 140 600 33 16 640 230

F5-1 76 350 16 12 320 130

F5-2 69 300 15 11 320 120

F5-3 110 430 23 14 R 390 150

All concentrations are in ug/kg, wet weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-7. Results of the PCB congener analyses of juvenile smallmouth bass

tissue samples collected in August 1997

PCB044 PCB045 PCB046 PCB047/048 PCB049 PCB052

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

F1-1 0.69 0.03 U 0.03 U 0.9 1.1 1.7

F1-2 0.54 0.02 U 0.02 U 1 1 1.6

F1-3 0.49 0.03 U 0.03 U 0.67 0.91 1.5

F2-1 440 >D 40 D 9 D 600 >D 540 >D 560 >D 

F2-2 490 D 39 D 7.7 D 690 >D 790 >D 910 >D 

F2-3 250 >D 24 D 5.8 D 480 >D 470 >D 500 >D 

F3-1 430 38 11 1000 950 970

F3-2 370 36 8.7 940 820 840

F3-3 340 35 8.9 960 820 840

F5-1 200 25 5.3 480 430 460

F5-2 190 24 4.9 430 390 440

F5-3 240 31 8.1 U 620 560 600

All concentrations are in ug/kg, wet weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-7. Results of the PCB congener analyses of juvenile smallmouth bass

tissue samples collected in August 1997

PCB056/060 PCB066 PCB070/076 PCB074 PCB077* PCB083

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

F1-1 0.55 1.5 2 1.4 0.107 0.36

F1-2 0.44 1.4 1.6 1.1 0.115 0.33

F1-3 0.44 1.4 1.7 1.2 0.1 0.29

F2-1 260 >D 490 >D 660 >D 620 >D 39.4 D 160 D 

F2-2 260 D 780 >D 1000 >D 900 >D 53 D 240 D 

F2-3 170 >D 420 >D 500 >D 510 >D 25.4 130 D 

F3-1 270 870 760 940 35.2 D 85

F3-2 250 750 610 840 24.4 74

F3-3 230 740 640 820 26.6 D 71

F5-1 120 320 280 370 10.3 38

F5-2 110 320 270 360 10.3 41

F5-3 120 360 270 410 10.5 45

All concentrations are in ug/kg, wet weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-7. Results of the PCB congener analyses of juvenile smallmouth bass

tissue samples collected in August 1997

PCB084/089 PCB085 PCB087 PCB090/101 PCB091 PCB095

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

F1-1 1.7 1.8 3.6 8.4 0.54 2.6

F1-2 1.5 1.6 3.1 7.7 0.53 2.5

F1-3 1.6 1.7 3.3 8.7 0.48 2.3

F2-1 280 D 740 D 1500 >D 590 >D 160 D 430 >D 

F2-2 280 D 830 D 1600 D 920 >D 170 D 500 >D 

F2-3 170 D 550 D 1100 >D 530 >D 100 D 290 >D 

F3-1 330 310 590 1100 200 580

F3-2 300 300 520 990 180 480

F3-3 300 260 480 950 180 490

F5-1 150 140 270 480 85 250

F5-2 150 150 280 500 91 250

F5-3 190 180 340 590 120 310

All concentrations are in ug/kg, wet weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-7. Results of the PCB congener analyses of juvenile smallmouth bass

tissue samples collected in August 1997

PCB097 PCB099 PCB105 PCB107 PCB110 PCB114*

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

F1-1 2.2 4.3 2.4 0.76 10 0.186 L 

F1-2 2.1 4.1 2.5 0.7 8.7 0.163 L 

F1-3 2.1 4.2 2.3 0.72 9.5 0.277

F2-1 1200 >D 520 >D 300 >D 70 D 2800 >D 28.7

F2-2 1400 D 630 >D 330 D 75 D 4200 >D 32.7

F2-3 810 D 410 >D 210 >D 53 D 2300 >D 24

F3-1 450 750 390 84 1700 34.5

F3-2 410 700 350 78 1500 33.3

F3-3 380 670 330 75 1400 28.9

F5-1 180 320 140 36 730 13.9

F5-2 200 330 160 41 820 17.4

F5-3 240 390 180 47 940 19.8

All concentrations are in ug/kg, wet weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-7. Results of the PCB congener analyses of juvenile smallmouth bass

tissue samples collected in August 1997

PCB118 PCB123* PCB126* PCB128 PCB129 PCB130

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

F1-1 7.8 0.0888 0.0144 2.6 0.46 0.14 U 

F1-2 7.9 0.0722 0.0169 2.6 0.59 0.07 U 

F1-3 7.5 0.152 0.0212 2.5 0.44 0.08 U 

F2-1 570 >D 11.7 1.07 130 D 25 D 0.49 RD 

F2-2 890 >D 11 1.51 98 D 24 D 0.27 RD 

F2-3 480 >D 10.9 0.786 88 D 18 D 0.18 RD 

F3-1 1100 13.2 1.59 150 31 2.9 U 

F3-2 970 13.9 1.29 170 28 3.9 U 

F3-3 970 11.5 1.45 170 32 4.6 U 

F5-1 420 6.17 0.394 64 12 2.2 U 

F5-2 460 8.08 0.522 81 14 4.2 U 

F5-3 520 6.51 0.554 90 17 4.2 U 

All concentrations are in ug/kg, wet weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-7. Results of the PCB congener analyses of juvenile smallmouth bass

tissue samples collected in August 1997

PCB131 PCB134 PCB136 PCB137 PCB138/163/164 PCB141

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

F1-1 0.16 U 0.27 0.58 0.57 13 1.4

F1-2 0.09 0.32 0.55 0.72 15 1.5

F1-3 0.16 0.25 0.56 0.62 12 1.3

F2-1 5.2 D 21 D 54 D 35 D 650 >D 68 D 

F2-2 5.6 D 20 D 56 D 35 D 600 D 65 D 

F2-3 3.5 D 14 D 33 D 26 D 460 >D 50 D 

F3-1 8.4 30 79 45 800 95

F3-2 6.6 30 78 44 830 95

F3-3 6.9 27 72 49 830 97

F5-1 2.7 15 40 20 340 36

F5-2 4.9 16 36 23 400 45

F5-3 5.4 U 26 49 25 450 54

All concentrations are in ug/kg, wet weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-7. Results of the PCB congener analyses of juvenile smallmouth bass

tissue samples collected in August 1997

PCB144/135 PCB146 PCB149 PCB151 PCB153 PCB156*

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

F1-1 1.1 1.5 5.8 1 9.9 0.958

F1-2 0.91 1.4 5.2 1.1 12 1.01

F1-3 0.94 1.5 5.6 0.97 9.5 0.874

F2-1 59 D 60 D 340 D 66 D 420 >D 51.4

F2-2 60 D 59 D 340 D 66 D 380 D 63

F2-3 44 D 53 D 270 D 47 D 290 D 45.1

F3-1 88 85 500 98 560 77.8

F3-2 86 85 490 100 590 67.8

F3-3 80 84 480 94 580 75.5

F5-1 42 43 250 48 250 33.7

F5-2 47 54 290 52 300 35.6

F5-3 63 69 360 66 310 37.2

All concentrations are in ug/kg, wet weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-7. Results of the PCB congener analyses of juvenile smallmouth bass

tissue samples collected in August 1997

PCB156/157* PCB157* PCB158 PCB167* PCB169* PCB170*

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

F1-1 1.38 0.167 1.5 0.495 0.00172 U 0.907

F1-2 1.64 0.186 1.6 0.573 0.00197 U 1.25

F1-3 1.50 0.217 1.5 0.553 0.00192 U 0.919

F2-1 76.6 10.9 76 D 18.7 0.00954 U 48.2

F2-2 79.1 10.5 72 D 19.9 0.00627 U 45.4

F2-3 60.4 8.16 52 D 15.7 0.00605 U 37.2

F3-1 99.7 14 92 30.8 0.00813 U 55.5

F3-2 83.5 14.5 96 22.1 0.01017 U 56.7

F3-3 85.5 13.7 97 22.7 0.00669 U 51.7

F5-1 36.3 5.17 36 10.5 0.00468 U 20.4

F5-2 46.3 6.29 46 14 0.00585 U 25.1

F5-3 45.8 6.63 45 13.3 0.00439 U 28.3

All concentrations are in ug/kg, wet weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-7. Results of the PCB congener analyses of juvenile smallmouth bass

tissue samples collected in August 1997

PCB170/190 PCB171 PCB172 PCB174 PCB175 PCB176

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

F1-1 1.2 0.22 0.16 0.67 0.04 R 0.14 R 

F1-2 1.3 0.29 0.21 0.72 0.05 0.14 R 

F1-3 1.2 0.23 0.19 0.72 0.03 R 0.14

F2-1 57 D 11 D 6.1 D 25 D 1.4 D 5.5 D 

F2-2 55 D 11 D 6 D 25 D 1.6 D 5.5 D 

F2-3 42 D 7.8 D 4.8 D 19 D 0.97 D 3.7 D 

F3-1 79 15 11 35 3 U 7.3

F3-2 79 17 9.3 40 2.7 R 7 R 

F3-3 74 18 9.6 38 3.9 9.8

F5-1 30 7.1 5.2 13 3.3 U 3.3 U 

F5-2 41 8.6 5.3 15 4.3 U 4.3 U 

F5-3 42 9 7.9 19 1.8 U 2.1 R 

All concentrations are in ug/kg, wet weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-7. Results of the PCB congener analyses of juvenile smallmouth bass

tissue samples collected in August 1997

PCB177 PCB178 PCB179 PCB180* PCB183 PCB185

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

F1-1 0.46 0.43 0.38 2.52 0.57 0.07

F1-2 0.54 0.43 0.38 2.82 0.71 0.09

F1-3 0.45 0.26 0.42 2.38 0.6 0.07

F2-1 20 D 7.9 D 15 D 82.3 20 D 2.8 D 

F2-2 19 D 7.8 D 15 D 75.4 19 D 2.8 D 

F2-3 15 D 6.2 D 8.6 D 72 14 D 1.9 D 

F3-1 31 13 21 98 25 6.1 R 

F3-2 30 12 22 97.7 28 5.4

F3-3 35 15 23 95 28 7.4

F5-1 14 6 7.5 39.8 12 3.8 U 

F5-2 16 6.4 7.6 48 15 4.9 U 

F5-3 20 8.9 11 59.5 18 2.9

All concentrations are in ug/kg, wet weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-7. Results of the PCB congener analyses of juvenile smallmouth bass

tissue samples collected in August 1997

PCB187/182 PCB189* PCB191 PCB193 PCB194 PCB195

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

F1-1 1.3 0.0524 0.03 0.12 0.25 R 0.13 U 

F1-2 1.5 0.0658 0.04 U 0.17 0.33 0.19

F1-3 1.4 0.0687 0.05 0.14 0.31 0.12

F2-1 44 D 2.16 1.8 D 4.3 D 14 D 3.8 D 

F2-2 41 D 2.35 1.8 D 4.5 D 16 D 3.6 D 

F2-3 37 D 1.8 1.4 RD 3.6 D 9.9 D 2.3 RD 

F3-1 70 2.99 3.3 U 5.8 22 3.8 U 

F3-2 74 2.84 3.6 5.5 19 5.9 U 

F3-3 87 3.18 4.7 7.8 20 6.9 U 

F5-1 37 1.17 3.7 U 4.8 7.2 2.8

F5-2 40 1.67 4.7 U 4.7 U 7.5 4.9 U 

F5-3 51 1.53 2 U 5.1 12 3.7 U 

All concentrations are in ug/kg, wet weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-7. Results of the PCB congener analyses of juvenile smallmouth bass

tissue samples collected in August 1997

PCB196/203 PCB197 PCB198 PCB199 PCB201 PCB205

Station Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

F1-1 0.4 0.14 U 0.14 U 0.41 0.07 U 0.15 U 

F1-2 0.54 0.14 U 0.14 U 0.56 0.07 U 0.15 U 

F1-3 0.45 0.11 U 0.11 U 0.54 0.07 0.12 U 

F2-1 12 D 0.93 RD 0.45 RD 11 D 0.95 D 0.79 RD 

F2-2 13 D 0.83 D 0.49 RD 12 D 0.95 D 1.5 RD 

F2-3 8.9 D 0.53 UD 0.53 UD 8.4 D 0.64 RD 0.98 UD 

F3-1 15 4.1 U 4.1 U 15 2 U 4.6 U 

F3-2 17 6.3 U 6.3 U 17 3 U 7.1 U 

F3-3 17 7.3 U 7.3 U 16 3.6 U 8.3 U 

F5-1 6.7 1.8 U 1.8 U 7.2 0.85 U 2 U 

F5-2 8.7 5.2 U 5.2 U 9.1 2.5 U 5.9 U 

F5-3 11 3.9 U 3.9 U 12 1.9 U 4.5 U 

All concentrations are in ug/kg, wet weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
Table A1-7. Results of the PCB congener analyses of juvenile smallmouth bass

tissue samples collected in August 1997

PCB206 PCB207 PCB208 PCB209 PCB Sum

Station Conc Q Conc Q Conc Q Conc Q Conc Q

F1-1 0.3 U 0.3 U 0.3 U 0.3 U 114.4

F1-2 0.4 U 0.4 U 0.4 U 0.4 UR 115.2

F1-3 0.4 U 0.4 U 0.4 U 0.4 U 108.9

F2-1 3.1 RD 1.2 UD 1.5 D 1.2 UD 18829.3

F2-2 3.1 RD 0.82 RD 1.2 RD 0.75 UD 23143.3

F2-3 1.8 RD 1.1 UD 1.1 UD 1.1 UD 14479.5

F3-1 5.1 R 4.9 U 4.9 U 3.2 U 20783.4

F3-2 3.6 R 1.8 U 1.8 U 3.9 U 18576.4

F3-3 6.4 U 6.4 U 6.4 U 5.1 U 18412.9

F5-1 3.1 U 3.1 U 3.1 U 3.3 U 9104.0

F5-2 4 U 4 U 4 U 2.6 U 9351.0

F5-3 2.6 U 2.6 U 2.6 U 3.6 U 11601.9

All concentrations are in ug/kg, wet weight, except where noted.

* PCB congeners analyzed under high resolution analysis method.
APPENDIX A-2

Quality Assurance Review Summaries

Chemical Analyses of Sediment

and Fish Tissue Samples

















APPENDIX A-3

WDNR Food Chain Study Results
LIST OF TABLES

Table A3-1. Results of the PCB analyses of sediment samples collected for the

WDNR food chain study

Table A3-2. Results of the PAH analyses of sediment samples collected for the

WDNR food chain study

Table A3-3. Results of the metals analyses of sediment samples collected for the

WDNR food chain study

Table A3-4. Results of the PCB analyses of tissue samples collected for the

WDNR food chain study

Table A3-5. Results of the PAH analyses of tissue samples collected for the

WDNR food chain study

Table A3-6. Results of the metals analyses of crayfish and emergent and larval

insect tissue samples collected for the WDNR food chain study

Table A3-7. Results of the PCB congener analyses of small mammal tissue

samples collected for the WDNR food chain study
Table A3-1. Continued

Station Sample Location

603363 6A Camp Marina

603364 6B Camp Marina

603365 6C Camp Marina

603366 6D Camp Marina

603367 6E Camp Marina

603368 5A Kiwanis Park

603369 5B Kiwanis Park

603370 5C Kiwanis Park

603371 5D Kiwanis Park

603372 3A Between Kohler Dams

603373 3B Between Kohler Dams

603374 3C Between Kohler Dams

603375 3D Between Kohler Dams

603376 3E Between Kohler Dams

603377 2A Rochester Park

603378 2B Rochester Park

603379 2C Rochester Park

603380 2D Rochester Park

603381 2E Rochester Park

603392 1A Control

603393 1B Control

603394 1C Control

603395 1D Control

603396 1E Control

PCB019 PCB022 PCB024/027 PCB026 PCB031/028 PCB033

(ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

7.5 12 14 33 150 21 U*I

11 13 18 44 160 34 U*I

6.8 15 17 38 180 25 U*I

8.7 14 15 34 160 23 U*I

5.9 7.9 8.3 27 98 16 U*I

7 19 20 49 200 30 U*I

8.4 17 16 47 210 32 U*I

3.9 8.2 6.5 18 110 12 U*I

6.2 14 11 29 150 20 U*I

21 20 33 50 240 42 U*I

24 27 30 73 220 43 U*I

21 16 27 42 180 31 U*I

71 26 81 99 320 71 U*I

51 35 57 98 370 64 U*I

30 23 32 47 210 37 U*I

43 65 44 100 470 74 U*I

46 26 50 63 330 51 U*I

270 89 280 300 1500 190 U*I

46 160 45 190 820 160 U*I

0.3 U 0.6 U 0.3 U 0.35 U 1.4 U 0.45 U 

0.3 U 0.6 U 0.3 U 0.35 U 1.4 U 0.45 U 

0.3 U 0.6 U 0.3 U 0.35 U 1.4 U 0.45 U 

0.3 U 0.6 U 0.3 U 0.35 U 1.4 U 0.45 U 

0.3 U 0.6 U 0.3 U 0.35 U 1.4 U 0.45 U 
Table A3-1. Continued

Station Sample Location

603363 6A Camp Marina

603364 6B Camp Marina

603365 6C Camp Marina

603366 6D Camp Marina

603367 6E Camp Marina

603368 5A Kiwanis Park

603369 5B Kiwanis Park

603370 5C Kiwanis Park

603371 5D Kiwanis Park

603372 3A Between Kohler Dams

603373 3B Between Kohler Dams

603374 3C Between Kohler Dams

603375 3D Between Kohler Dams

603376 3E Between Kohler Dams

603377 2A Rochester Park

603378 2B Rochester Park

603379 2C Rochester Park

603380 2D Rochester Park

603381 2E Rochester Park

603392 1A Control

603393 1B Control

603394 1C Control

603395 1D Control

603396 1E Control

PCB037/042 PCB040 PCB041/071/006 PCB044 PCB045 PCB046

(ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

32 7.3 60 45 6.2 2.4

36 8.8 64 52 6.9 5.9

36 9.9 76 58 8.1 3.4

34 9.5 72 56 7.8 3.4

22 5.6 40 32 4.3 2.4

43 11 87 62 8.5 3.8

44 12 83 64 8.8 4.9

19 5.2 45 35 4.4 1.6

33 8.8 74 59 7.4 2.8

59 16 130 100 16 5.8

67 19 130 110 18 7.2

37 9.9 78 54 11 4.5

74 19 160 110 27 8.4

84 25 180 140 29 9.6

49 16 110 87 17 6.1

110 39 250 200 38 15

56 18 120 98 20 6.6

220 57 510 340 92 30

250 100 530 500 84 41

0.4 U 0.3 U 0.5 U 0.3 U 0.3 U 0.35 U 

0.4 U 0.3 U 0.5 U 0.3 U 0.3 U 0.35 U 

0.4 U 0.3 U 0.5 U 0.3 U 0.3 U 0.35 U 

0.4 U 0.3 U 0.5 U 0.3 U 0.3 U 0.44 U*I

0.4 U 0.3 U 0.5 U 0.3 U 0.3 U 0.35 U 
Table A3-1. Continued

Station Sample Location

603363 6A Camp Marina

603364 6B Camp Marina

603365 6C Camp Marina

603366 6D Camp Marina

603367 6E Camp Marina

603368 5A Kiwanis Park

603369 5B Kiwanis Park

603370 5C Kiwanis Park

603371 5D Kiwanis Park

603372 3A Between Kohler Dams

603373 3B Between Kohler Dams

603374 3C Between Kohler Dams

603375 3D Between Kohler Dams

603376 3E Between Kohler Dams

603377 2A Rochester Park

603378 2B Rochester Park

603379 2C Rochester Park

603380 2D Rochester Park

603381 2E Rochester Park

603392 1A Control

603393 1B Control

603394 1C Control

603395 1D Control

603396 1E Control

PCB047/048 PCB049 PCB052 PCB056/060 PCB066/095 PCB070/076

(ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

120 57 62 48 100 63

160 73 74 42 92 60

130 65 73 56 120 78

130 62 69 54 120 75

74 41 45 30 64 42

160 77 84 62 130 83

150 85 87 56 130 82

67 36 42 38 78 53

110 57 69 60 120 80

250 110 120 92 200 110

220 110 140 71 160 91

170 67 73 43 98 57

410 150 150 76 230 100

370 150 170 94 250 130

200 86 99 70 150 98

340 180 210 160 320 200

250 100 110 69 150 100

1300 430 480 200 780 280

500 360 480 350 700 550

0.5 U 0.3 U 0.3 U 0.8 U 0.6 U 0.45 U 

0.5 U 0.3 U 0.3 U 0.8 U 0.7 0.53

0.5 U 0.3 U 0.3 U 0.8 U 0.71 U*I 0.53 U*I

0.5 U 0.3 U 0.38 U*I 0.8 U 0.88 0.89 U*I

0.5 U 0.3 U 0.3 U 0.8 U 0.81 0.9 U*I
Table A3-1. Continued

Station Sample Location

603363 6A Camp Marina

603364 6B Camp Marina

603365 6C Camp Marina

603366 6D Camp Marina

603367 6E Camp Marina

603368 5A Kiwanis Park

603369 5B Kiwanis Park

603370 5C Kiwanis Park

603371 5D Kiwanis Park

603372 3A Between Kohler Dams

603373 3B Between Kohler Dams

603374 3C Between Kohler Dams

603375 3D Between Kohler Dams

603376 3E Between Kohler Dams

603377 2A Rochester Park

603378 2B Rochester Park

603379 2C Rochester Park

603380 2D Rochester Park

603381 2E Rochester Park

603392 1A Control

603393 1B Control

603394 1C Control

603395 1D Control

603396 1E Control

PCB074 PCB077 PCB077/110 PCB082 PCB084/092 PCB085

(ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

31 5.8 92 8.2 42 10

29 5.6 93 7.3 44 8.3

39 8 110 10 52 12

36 7.9 100 10 50 12

20 4 59 5 27 6.9

44 7.3 120 11 57 12

40 7.5 120 10 60 15

25 4.6 69 6.8 30 10

39 6.2 98 9.9 47 15

61 10 190 18 99 30

51 8.6 170 14 94 22

35 5.2 100 7.6 52 11

57 9 210 13 110 18

69 9.8 230 16 130 26

47 7 130 12 75 22

110 15 280 29 160 52

51 7.3 150 11 79 20

180 27 720 46 390 79

220 30 590 69 350 120

0.85 U*I 0.2 U 0.53 0.3 U 0.7 U 0.76 U*I

0.65 U*I 0.2 U 0.82 0.3 U 0.7 U 0.79 U*I

0.95 U*I 0.2 U 0.68 0.3 U 0.7 U 0.65 U*I

0.86 U*I 0.2 U 1.2 0.3 U 0.7 U 1.3 U*I

0.54 U*I 0.2 U 1 0.3 U 0.7 U 1.1 U*I
Table A3-1. Continued

Station Sample Location

603363 6A Camp Marina

603364 6B Camp Marina

603365 6C Camp Marina

603366 6D Camp Marina

603367 6E Camp Marina

603368 5A Kiwanis Park

603369 5B Kiwanis Park

603370 5C Kiwanis Park

603371 5D Kiwanis Park

603372 3A Between Kohler Dams

603373 3B Between Kohler Dams

603374 3C Between Kohler Dams

603375 3D Between Kohler Dams

603376 3E Between Kohler Dams

603377 2A Rochester Park

603378 2B Rochester Park

603379 2C Rochester Park

603380 2D Rochester Park

603381 2E Rochester Park

603392 1A Control

603393 1B Control

603394 1C Control

603395 1D Control

603396 1E Control

PCB087 PCB091 PCB097 PCB099 PCB101 PCB105

(ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

23 24 18 26 38 24

18 34 16 23 39 19

28 28 22 31 45 30

27 27 21 29 44 29

14 16 11 15 23 15

30 32 23 33 47 34

28 33 23 32 48 30

20 15 14 19 29 20

28 24 21 28 41 29

48 52 39 53 79 47

38 48 33 45 65 34

21 32 19 27 40 19

37 75 37 55 85 33

45 72 44 62 94 37

34 39 29 39 57 29

81 74 65 79 120 66

31 42 29 40 59 25

140 230 140 210 300 100

210 140 150 170 280 160

0.62 0.4 U 0.3 U 0.32 U*I 0.3 U 0.2 U 

0.63 0.4 U 0.3 U 0.3 U 0.3 U 0.23

0.63 0.4 U 0.3 U 0.36 U*I 0.3 U 0.2 U 

0.69 0.4 U 0.3 U 0.7 U*I 0.3 U 0.34

0.7 0.4 U 0.3 U 0.61 U*I 0.3 U 0.28
Table A3-1. Continued

Station Sample Location

603363 6A Camp Marina

603364 6B Camp Marina

603365 6C Camp Marina

603366 6D Camp Marina

603367 6E Camp Marina

603368 5A Kiwanis Park

603369 5B Kiwanis Park

603370 5C Kiwanis Park

603371 5D Kiwanis Park

603372 3A Between Kohler Dams

603373 3B Between Kohler Dams

603374 3C Between Kohler Dams

603375 3D Between Kohler Dams

603376 3E Between Kohler Dams

603377 2A Rochester Park

603378 2B Rochester Park

603379 2C Rochester Park

603380 2D Rochester Park

603381 2E Rochester Park

603392 1A Control

603393 1B Control

603394 1C Control

603395 1D Control

603396 1E Control

PCB118 PCB123 PCB126 PCB128 PCB132/153 PCB136

(ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

45 2.3 U*I 0.26 6.3 35 1.9

39 2 U*I 0.2 U 5.6 34 2.1

55 2.6 U*I 0.41 U*I 8 42 2

53 2.9 U*I 0.3 U*I 8 42 2.1

26 2.6 U*I 0.2 U 3.7 21 1.3

59 3 U*I 0.2 U 8.7 44 2.1

55 3 U*I 0.23 U*I 8.6 44 3.2

35 1.1 U*I 0.2 U 5.1 26 1.5

48 1.4 U*I 0.2 U 6.8 35 2.2

89 3.3 U*I 0.27 13 76 6

68 2.5 U*I 0.36 U*I 11 62 5.6

40 3.5 U*I 0.23 U*I 5.9 42 3

79 4.7 U*I 0.2 U 12 86 7.7

88 4.9 U*I 0.35 U*I 14 92 8.6

59 2.3 U*I 0.26 U*I 8.7 51 4.7

120 3.3 U*I 0.6 U*I 17 89 8.6

60 2.7 U*I 0.22 U*I 8.4 52 4.6

290 15 U*I 1.2 U*I 47 330 31

290 9 U*I 0.65 U*I 47 220 20

0.45 U 0.2 U 0.22 U 0.5 U 0.56 0.38 U*I

0.54 0.2 U 0.2 U 0.5 U 0.82 0.4 U*I

0.46 0.2 U 0.2 U 0.5 U 0.71 0.32 U*I

0.82 0.2 U 0.2 U 0.5 U 1.3 0.64 U*I

0.76 0.2 U 0.2 U 0.5 U 1.2 0.55 U*I
Table A3-1. Continued

Station Sample Location

603363 6A Camp Marina

603364 6B Camp Marina

603365 6C Camp Marina

603366 6D Camp Marina

603367 6E Camp Marina

603368 5A Kiwanis Park

603369 5B Kiwanis Park

603370 5C Kiwanis Park

603371 5D Kiwanis Park

603372 3A Between Kohler Dams

603373 3B Between Kohler Dams

603374 3C Between Kohler Dams

603375 3D Between Kohler Dams

603376 3E Between Kohler Dams

603377 2A Rochester Park

603378 2B Rochester Park

603379 2C Rochester Park

603380 2D Rochester Park

603381 2E Rochester Park

603392 1A Control

603393 1B Control

603394 1C Control

603395 1D Control

603396 1E Control

PCB137/176 PCB138/163 PCB141 PCB144/135 PCB146 PCB149

(ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

2.2 U*I 39 4.2 U 5.6 6.8 23

2 U*I 39 4 U*I 6.7 8.5 24

2.7 U*I 48 5.3 U*I 6.8 8.4 27

2.7 U*I 47 5.3 U*I 6.5 8.3 27

1.4 U*I 23 2.6 U*I 3.1 3.7 13

3 U*I 52 5.6 U*I 7.3 9 29

50 5.4 U*I 7.5 8.8 30

1.9 U*I 31 3.5 U*I 3.7 4.7 16

3.4 U*I 41 4.8 U*I 5.4 6.7 23

4.7 U*I 83 9.1 U*I 13 15 51

66 7.6 U*I 11 13 42

2.2 U*I 40 4.1 U*I 7.4 9.2 28

82 8.2 U*I 18 20 63

90 9 U*I 18 21 64

53 6.1 U*I 9.1 11 33

100 13 U*I 14 17 57

50 5.5 U*I 9 11 34

290 33 U*I 57 70 200

260 36 U*I 31 39 130

0.3 U 0.57 0.3 U 0.3 U 0.35 U 0.3 U 

0.45 U*I 0.86 0.3 U 0.3 U 0.35 U 0.33

0.39 U*I 0.74 0.3 U 0.3 U 0.35 U 0.3 U 

0.3 U*I 1.4 0.3 U 0.3 U 0.35 U 0.5

0.3 U 1.3 0.3 U 0.3 U 0.35 U 0.48
Table A3-1. Continued

Station Sample Location

603363 6A Camp Marina

603364 6B Camp Marina

603365 6C Camp Marina

603366 6D Camp Marina

603367 6E Camp Marina

603368 5A Kiwanis Park

603369 5B Kiwanis Park

603370 5C Kiwanis Park

603371 5D Kiwanis Park

603372 3A Between Kohler Dams

603373 3B Between Kohler Dams

603374 3C Between Kohler Dams

603375 3D Between Kohler Dams

603376 3E Between Kohler Dams

603377 2A Rochester Park

603378 2B Rochester Park

603379 2C Rochester Park

603380 2D Rochester Park

603381 2E Rochester Park

603392 1A Control

603393 1B Control

603394 1C Control

603395 1D Control

603396 1E Control

PCB151 PCB156 PCB157 PCB167 PCB169 PCB170/190

(ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

6 4.1 1.1 1.4 0.2 U 8.4

8 3.2 0.93 1.2 0.2 U 12

7.2 4.6 1.4 1.7 0.2 U 10

7.1 4.5 1.3 1.7 0.2 U 9.9

3.5 2.3 0.69 0.85 0.2 U 5.2

7.8 5.3 1.4 1.9 0.2 U 11

8 4.9 1.5 1.8 0.2 U 11

3.9 3 0.92 1 0.2 U 5.7

5.9 3.9 1.2 1.4 0.2 U 8.2

13 7.9 2.3 2.8 0.2 U 18

12 6.7 2 2.4 0.2 U 15

7.5 3.5 1.1 1.3 0.2 U 10

18 7.1 2.2 2.7 0.2 U 19

18 7.9 2.3 2.8 0.25 U*D 19

9 5 1.6 1.9 0.2 U 11

15 10 3 3.5 0.27 U*D 21

9.2 4.5 1.3 1.8 0.2 U 11

56 26 8 9.8 0.64 U*D 63

33 27 8.1 9.3 0.5 U*D 48

0.3 U 0.2 U 0.2 U 0.5 U 0.2 U 0.7 U 

0.3 U 0.2 U 0.2 U 0.5 U 0.2 U 0.7 U 

0.3 U 0.2 U 0.2 U 0.5 U 0.2 U 0.7 U 

0.3 U 0.2 U 0.2 U 0.5 U 0.2 U 0.7 U 

0.3 U 0.2 U 0.2 U 0.5 U 0.2 U 0.7 U 
Table A3-1. Continued

Station Sample Location

603363 6A Camp Marina

603364 6B Camp Marina

603365 6C Camp Marina

603366 6D Camp Marina

603367 6E Camp Marina

603368 5A Kiwanis Park

603369 5B Kiwanis Park

603370 5C Kiwanis Park

603371 5D Kiwanis Park

603372 3A Between Kohler Dams

603373 3B Between Kohler Dams

603374 3C Between Kohler Dams

603375 3D Between Kohler Dams

603376 3E Between Kohler Dams

603377 2A Rochester Park

603378 2B Rochester Park

603379 2C Rochester Park

603380 2D Rochester Park

603381 2E Rochester Park

603392 1A Control

603393 1B Control

603394 1C Control

603395 1D Control

603396 1E Control

PCB171/202 PCB172/197 PCB174 PCB177 PCB178 PCB180

(ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

1.1 1.1 3 2.9 1.1 7.5

2 2 3.9 4.7 2.8 12

1.4 U*I 1.3 3.4 3.6 1.3 8.2

1.4 U*I 1.3 2.8 3.2 1.4 8.1

0.74 1.9 1.6 0.64 4.6

1.4 1.4 3.5 3.6 1.5 9

1.4 U*I 1.3 2.5 U*I 3.4 1.5 8.7

1.9 1.7 0.63 4.6

0.96 1 2.7 2.5 0.92 6.6

2.4 2.2 5.9 6.5 2.4 14

1.9 1.9 4.7 5.2 2.1 12

1.4 1.3 3 3.7 1.5 8

2.9 2.5 4.9 8.2 3.7 15

2.8 U*I 2.5 4.7 5.9 3.6 16

1.7 U*I 1.6 3.8 4.3 1.8 10

2.9 U*I 2.6 7.3 7.1 2.4 17

1.6 U*I 1.4 3.6 U*I 3.9 2 9.1

7.2 7.6 19 22 8.2 45

6.1 5.9 16 13 4.1 38

0.3 U 0.5 U 0.3 U 0.35 U 0.4 U 0.35 U 

0.3 U 0.5 U 0.3 U 0.35 U 0.4 U 0.35 U 

0.3 U 0.5 U 0.3 U 0.35 U 0.4 U 0.35 U 

0.3 U 0.5 U 0.3 U 0.35 U 0.4 U 0.35 U 

0.3 U 0.5 U 0.3 U 0.35 U 0.4 U 0.35 U 
Table A3-1. Continued

Station Sample Location

603363 6A Camp Marina

603364 6B Camp Marina

603365 6C Camp Marina

603366 6D Camp Marina

603367 6E Camp Marina

603368 5A Kiwanis Park

603369 5B Kiwanis Park

603370 5C Kiwanis Park

603371 5D Kiwanis Park

603372 3A Between Kohler Dams

603373 3B Between Kohler Dams

603374 3C Between Kohler Dams

603375 3D Between Kohler Dams

603376 3E Between Kohler Dams

603377 2A Rochester Park

603378 2B Rochester Park

603379 2C Rochester Park

603380 2D Rochester Park

603381 2E Rochester Park

603392 1A Control

603393 1B Control

603394 1C Control

603395 1D Control

603396 1E Control

PCB183 PCB185 PCB187/182 PCB194 PCB195/208 PCB196/203

(ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg) (ug/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

2.3 3 1.5 1.7 2.5

3.5 6.8 5.2 5.1 7.7

2.7 2.8 1.5 1.9 2.6

2.8 2.6 1.4 1.7 2.5

1.4 2 0.95 1.6

2.8 3.8 1.6 2 2.8

2.9 3.8 1.4 1.7 2.4

1.5 2.1 0.69

2.1 3.3 1 1.4 1.8

4.7 5 2.8 3.2 4.6

4 0.58 5.5 2.2 2.6 3.8

2.6 4 1.6 1.9 2.8

5.2 8.6 3.3 3.6 5.8

5.4 5.1 2.9 3.5 5

3.3 2.9 1.9 2.2 3.3

5.8 7.4 3 3.6 5.1

3.1 2.4 U*I 1.6 2 2.6

16 28 6.4 7.8 12

13 16 4.4 5.3 U*I 8

0.4 U 0.3 U 0.4 U 0.5 U 0.7 U 0.7 U 

0.4 U 0.3 U 0.4 U 0.5 U 0.7 U 0.7 U 

0.4 U 0.3 U 0.53 U*I 0.5 U 0.7 U 0.7 U 

0.4 U 0.3 U 0.4 U 0.5 U 0.7 U 0.7 U 

0.4 U 0.3 U 0.4 U 0.5 U 0.7 U 0.7 U 
Table A3-1. Continued

Station Sample Location

603363 6A Camp Marina

603364 6B Camp Marina

603365 6C Camp Marina

603366 6D Camp Marina

603367 6E Camp Marina

603368 5A Kiwanis Park

603369 5B Kiwanis Park

603370 5C Kiwanis Park

603371 5D Kiwanis Park

603372 3A Between Kohler Dams

603373 3B Between Kohler Dams

603374 3C Between Kohler Dams

603375 3D Between Kohler Dams

603376 3E Between Kohler Dams

603377 2A Rochester Park

603378 2B Rochester Park

603379 2C Rochester Park

603380 2D Rochester Park

603381 2E Rochester Park

603392 1A Control

603393 1B Control

603394 1C Control

603395 1D Control

603396 1E Control

PCB199 PCB201 PCB206

(ug/kg) (ug/kg) (ug/kg)

Conc Q Conc Q Conc Q

2.2 0.84

6.3 2.5

2.2

2.1

1.4

2.5 1

2

1

1.6

3.8 1.9 U*I

3.1 0.86

2.3

4.8

4.2

2.8 1.1

4.3

2.5

9.8

6.2

0.3 U 0.5 U 0.4 U 

0.3 U 0.5 U 0.4 U 

0.3 U 0.5 U 0.4 U 

0.3 U 0.5 U 0.4 U 

0.3 U 0.5 U 0.4 U 
Table A3-2. Results of the PAH analyses of sediment samples collected for the WDNR food chain study

Organic carbon, total Acenaphthene Acenaphthylene Anthracene

(mg/kg) (ug/kg) (ug/kg) (ug/kg)

Station Sample Location Conc Q Conc Q Conc Q Conc Q

603363 6A Camp Marina 21300 100 U 100 U 110

603364 6B Camp Marina 24000

603365 6C Camp Marina 33200 220 100 U 240

603365 6DUP Camp Marina 400 110 460

603365 6DUPPAH Camp Marina 66500 400000 16000 330000

603366 6D Camp Marina 35900 220 220 300

603367 6E Camp Marina 9300

603368 5A Kiwanis Park 35100 100 U 100 U 130

603368 5DUP Kiwanis Park 100 U 100 U 100 U 

603369 5B Kiwanis Park 10200 100 U 100 U 100 U 

603370 5C Kiwanis Park 8740 100 U 100 U 100 U 

603371 5D Kiwanis Park 12500

603372 3A Between Kohler Dams 20100 100 U 100 U 100 U 

603373 3B Between Kohler Dams 20800

603374 3C Between Kohler Dams 10200

603375 3D Between Kohler Dams 30800 100 U 100 U 100 U 

603375 3DUP Between Kohler Dams 100 U 100 U 100 U 

603376 3E Between Kohler Dams 33100 100 U 100 U 100 U 

603377 2A Rochester Park 23100 100 U 100 U 100 U 

603378 2B Rochester Park 33600

603379 2C Rochester Park 19500 100 U 100 U 100 U 

603380 2D Rochester Park 16200 500 U*D 500 U*D 670

603381 2E Rochester Park 14100

603392 1A Control 22100

603393 1B Control 22600 100 U 100 U 100 U 

603394 1C Control 23200

603395 1D Control 40900 100 U 100 U 100 U 

603396 1E Control 34300 100 U 100 U 100 U 
Table A3-2. Results of the PAH analyses of sediment samples collected for the WDNR food chain study

Station Sample Location

603363 6A Camp Marina

603364 6B Camp Marina

603365 6C Camp Marina

603365 6DUP Camp Marina

603365 6DUPPAH Camp Marina

603366 6D Camp Marina

603367 6E Camp Marina

603368 5A Kiwanis Park

603368 5DUP Kiwanis Park

603369 5B Kiwanis Park

603370 5C Kiwanis Park

603371 5D Kiwanis Park

603372 3A Between Kohler Dams

603373 3B Between Kohler Dams

603374 3C Between Kohler Dams

603375 3D Between Kohler Dams

603375 3DUP Between Kohler Dams

603376 3E Between Kohler Dams

603377 2A Rochester Park

603378 2B Rochester Park

603379 2C Rochester Park

603380 2D Rochester Park

603381 2E Rochester Park

603392 1A Control

603393 1B Control

603394 1C Control

603395 1D Control

603396 1E Control

Benz(a)anthracene Dibenz(a,h)anthracene Benzo(a)pyrene Benzo(b)fluoranthene

(ug/kg) (ug/kg) (ug/kg) (ug/kg)

Conc Q Conc Q Conc Q Conc Q

420 100 U 400 440

800 100 U 600 760

1400 100 U 820 860

180000 13000 210000 170000

1800 100 U 1000 1200

730 100 U 640 770

420 100 U 440 580

100 U 100 U 100 U 100 U 

100 U 100 U 100 U 100 U 

100 U 100 U 100 U 100 U 

120 100 U 110 180

120 100 U 120 190

100 U 100 U 170 100 U 

100 U 100 U 100 U 100 U 

100 U 100 U 100 U 130

1800 500 U*D 1500 2100

100 U 100 U 100 U 100 U 

100 U 100 U 100 U 100 U 

100 U 100 U 100 U 100 U 
Table A3-2. Results of the PAH analyses of sediment samples collected for the WDNR food chain study

Station Sample Location

603363 6A Camp Marina

603364 6B Camp Marina

603365 6C Camp Marina

603365 6DUP Camp Marina

603365 6DUPPAH Camp Marina

603366 6D Camp Marina

603367 6E Camp Marina

603368 5A Kiwanis Park

603368 5DUP Kiwanis Park

603369 5B Kiwanis Park

603370 5C Kiwanis Park

603371 5D Kiwanis Park

603372 3A Between Kohler Dams

603373 3B Between Kohler Dams

603374 3C Between Kohler Dams

603375 3D Between Kohler Dams

603375 3DUP Between Kohler Dams

603376 3E Between Kohler Dams

603377 2A Rochester Park

603378 2B Rochester Park

603379 2C Rochester Park

603380 2D Rochester Park

603381 2E Rochester Park

603392 1A Control

603393 1B Control

603394 1C Control

603395 1D Control

603396 1E Control

Benzo(e)pyrene Benzo(g,h,i)perylene Benzo(k)fluoranthene Chrysene

(ug/kg) (ug/kg) (ug/kg) (ug/kg)

Conc Q Conc Q Conc Q Conc Q

320 160 220 380

480 100 U 380 600

560 100 U 420 920

90000 43000 67000 130000

790 380 480 1400

480 190 320 700

400 160 240 400

100 U 100 U 100 U 100 U 

100 U 100 U 100 U 100 U 

100 U 100 U 100 U 100 U 

100 U 100 U 100 U 100

100 U 100 U 100 U 110

180 100 U 100 U 130

100 U 100 U 100 U 100 U 

100 U 100 U 100 U 100 U 

1000 540 920 1600

100 U 100 U 100 U 100 U 

100 U 100 U 100 U 100 U 

100 U 100 U 100 U 100 U 
Table A3-2. Results of the PAH analyses of sediment samples collected for the WDNR food chain study

Station Sample Location

603363 6A Camp Marina

603364 6B Camp Marina

603365 6C Camp Marina

603365 6DUP Camp Marina

603365 6DUPPAH Camp Marina

603366 6D Camp Marina

603367 6E Camp Marina

603368 5A Kiwanis Park

603368 5DUP Kiwanis Park

603369 5B Kiwanis Park

603370 5C Kiwanis Park

603371 5D Kiwanis Park

603372 3A Between Kohler Dams

603373 3B Between Kohler Dams

603374 3C Between Kohler Dams

603375 3D Between Kohler Dams

603375 3DUP Between Kohler Dams

603376 3E Between Kohler Dams

603377 2A Rochester Park

603378 2B Rochester Park

603379 2C Rochester Park

603380 2D Rochester Park

603381 2E Rochester Park

603392 1A Control

603393 1B Control

603394 1C Control

603395 1D Control

603396 1E Control

Fluoranthene Fluorene Indeno(1,2,3-c,d)pyrene Perylene

(ug/kg) (ug/kg) (ug/kg) (ug/kg)

Conc Q Conc Q Conc Q Conc Q

1200 100 U 140 110

1900 170 250 170

2400 320 260 240

290000 250000 56000 27000

2200 190 460 260

2400 100 U 260 150

1600 100 U 200 120

190 100 U 100 U 100 U 

130 100 U 100 U 100 U 

120 100 U 100 U 100 U 

300 100 U 100 U 100 U 

310 100 U 100 U 100 U 

240 100 U 100 U 120

160 100 U 100 U 100 U 

160 100 U 100 U 100 U 

5200 500 U*D 680 500 U*D 

100 U 100 U 100 U 100 U 

100 U 100 U 100 U 100 U 

100 U 100 U 100 U 100 U 
Table A3-2. Results of the PAH analyses of sediment samples collected for the WDNR food chain study

Station Sample Location

603363 6A Camp Marina

603364 6B Camp Marina

603365 6C Camp Marina

603365 6DUP Camp Marina

603365 6DUPPAH Camp Marina

603366 6D Camp Marina

603367 6E Camp Marina

603368 5A Kiwanis Park

603368 5DUP Kiwanis Park

603369 5B Kiwanis Park

603370 5C Kiwanis Park

603371 5D Kiwanis Park

603372 3A Between Kohler Dams

603373 3B Between Kohler Dams

603374 3C Between Kohler Dams

603375 3D Between Kohler Dams

603375 3DUP Between Kohler Dams

603376 3E Between Kohler Dams

603377 2A Rochester Park

603378 2B Rochester Park

603379 2C Rochester Park

603380 2D Rochester Park

603381 2E Rochester Park

603392 1A Control

603393 1B Control

603394 1C Control

603395 1D Control

603396 1E Control

Phenanthrene Pyrene

(ug/kg) (ug/kg)

Conc Q Conc Q

560 1000

1200 1900

2000 2800

840000 340000

1300 2900

940 2100

600 1300

160 140

100 U 100 U 

100 U 100 U 

160 220

180 230

130 200

100 U 120

100 U 120

3500 4000

100 U 100 U 

100 U 100 U 

100 U 100 U 
Table A3-4. Continued

Species Age Class Station Sample Location

Crayfish 603384 9409 Control

Crayfish 603384 9410 Control

Crayfish 603384 9411 Control

Crayfish 603385 9409 Rochester Park

Crayfish 603385 9410 Rochester Park

Crayfish 603385 9411 Rochester Park

Crayfish 603386 9409 Between Kohler Dams

Crayfish 603386 9410 Between Kohler Dams

Crayfish 603386 9411 Between Kohler Dams

Crayfish 603387 9409 Esselingen Park

Crayfish 603387 9410 Esselingen Park

Crayfish 603387 9411 Esselingen Park

Emergent Insects 603384 9401 Control

Emergent Insects 603384 9402 Control

Emergent Insects 603384 9403 Control

Emergent Insects 603384 9404 Control

Emergent Insects 603385 9401 Rochester Park

Emergent Insects 603385 9402 Rochester Park

Emergent Insects 603385 9403 Rochester Park

Emergent Insects 603385 9404 Rochester Park

Emergent Insects 603386 9401 Between Kohler Dams

Emergent Insects 603386 9402 Between Kohler Dams

Emergent Insects 603386 9403 Between Kohler Dams

Emergent Insects 603386 9404 Between Kohler Dams

Emergent Insects 603387 9401 Esselingen Park

Emergent Insects 603387 9402 Esselingen Park

Emergent Insects 603387 9403 Esselingen Park

Emergent Insects 603387 9404 Esselingen Park

Larval Insects 603384 9405 Control

Larval Insects 603384 9406 Control

Larval Insects 603384 9407 Control

Larval Insects 603384 9408 Control

Larval Insects 603385 9405 Rochester Park

Larval Insects 603385 9406 Rochester Park

Larval Insects 603385 9407 Rochester Park

Larval Insects 603385 9408 Rochester Park

Larval Insects 603386 9405 Between Kohler Dams

Larval Insects 603386 9406 Between Kohler Dams

Larval Insects 603386 9407 Between Kohler Dams

Larval Insects 603386 9408 Between Kohler Dams

Larval Insects 603387 9406 Esselingen Park

Larval Insects 603387 9407 Esselingen Park

Larval Insects 603387 9408 Esselingen Park

PCB018 PCB019 PCB022 PCB024/027 PCB026

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

1 U 1 U 1.2 U 1 U 0.8 U 

1 U 1 U 1.2 U 1 U 0.8 U 

1 U 1 U 1.2 U 1 U 0.8 U 

3.4 9.5 23

11 22

3.5 9.5 25

3.3 1 14 2.7 34

3.6 15 3.3 36

3.4 13 2.6 38

1.7 1 U 6.9 1.1 22

1.9 1 U 7.8 1 U 25

2 6.2 25

1 U 1 U 1.2 U 1 U 1.1 U*I

1 U 1 U 1.2 U 1 U 0.8 U 

1 U 1 U 1.2 U 1 U 0.8 U 

1 U 1 U 1.2 U 1 U 0.8 U 

64 49 58 64 130

110 54 120 84 230

68 31 59 61 120

68 43 57 69 130

87 100 67 150 240

74 73 68 130 260

36 31 40 67 120

53 54 45 97 140

33 37 54 54 110

27 22 29 43 87

33 25 39 52 110

39 11 40 70 140

1 U 1 U 1.2 U 1 U 0.8 U 

1 U 1 U 1.2 U 1 U 0.8 U 

1 U 1 U 1.2 U 1 U 0.8 U 

1 U 1 U 1.2 U 1 U 0.8 U 

79 15 93 49 110

47 6.2 77 31 86

70 17 85 46 100

46 28 70 38 93

66 68 68 110 160

54 33 63 100 160

51 55 62 96 140

42 43 62 77 130

30 32 45 51 95

32 28 53 55 100

31 25 54 54 110
Table A3-4. Continued

Species Age Class Station Sample Location

Longnose Dace Young of year 603388 9413 Control

Longnose Dace Young of year 603388 9414 Control

Longnose Dace Young of year 603388 9415 Control

Longnose Dace Young of year 603389 9413 Rochester Park

Longnose Dace Young of year 603389 9414 Rochester Park

Longnose Dace Young of year 603389 9415 Rochester Park

Longnose Dace Young of year 603390 9413 Esselingen Park

Longnose Dace Young of year 603390 9414 Esselingen Park

Longnose Dace Young of year 603390 9415 Esselingen Park

Longnose Dace Young of year 603391 9413 Between Kohler Dams

Longnose Dace Young of year 603391 9414 Between Kohler Dams

Longnose Dace Young of year 603391 9415 Between Kohler Dams

Smallmouth Bass Adult 603388 9401 Control

Smallmouth Bass Adult 603388 9402 Control

Smallmouth Bass Adult 603388 9403 Control

Smallmouth Bass Young of year 603388 9404 Control

Smallmouth Bass Young of year 603388 9405 Control

Smallmouth Bass Young of year 603388 9406 Control

Smallmouth Bass Adult 603389 9401 Rochester Park

Smallmouth Bass Adult 603389 9402 Rochester Park

Smallmouth Bass Adult 603389 9403 Rochester Park

Smallmouth Bass Young of year 603389 9404 Rochester Park

Smallmouth Bass Young of year 603389 9405 Rochester Park

Smallmouth Bass Young of year 603389 9406 Rochester Park

Smallmouth Bass Adult 603390 9401 Esselingen Park

Smallmouth Bass Adult 603390 9402 Esselingen Park

Smallmouth Bass Adult 603390 9403 Esselingen Park

Smallmouth Bass Young of year 603390 9404 Esselingen Park

Smallmouth Bass Young of year 603390 9405 Esselingen Park

Smallmouth Bass Young of year 603390 9406 Esselingen Park

Smallmouth Bass Adult 603391 9401 Between Kohler Dams

Smallmouth Bass Adult 603391 9402 Between Kohler Dams

Smallmouth Bass Adult 603391 9403 Between Kohler Dams

Smallmouth Bass Young of year 603391 9404 Between Kohler Dams

Smallmouth Bass Young of year 603391 9405 Between Kohler Dams

Smallmouth Bass Young of year 603391 9406 Between Kohler Dams

White Sucker Adult 603388 9407 Control

White Sucker Adult 603388 9408 Control

White Sucker Adult 603388 9409 Control

White Sucker Young of year 603388 9410 Control

White Sucker Young of year 603388 9411 Control

White Sucker Young of year 603388 9412 Control

White Sucker Adult 603389 9407 Rochester Park

PCB018 PCB019 PCB022 PCB024/027 PCB026

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

1.2 1 U 1.6 1 U 2.5

1 U 1 U 1.2 U 1 U 0.8 U 

1 U 1 U 1.2 U 1 U 0.8 U 

44 63 38 69 58

45 67 42 77 65

66 51 63 120 95

67 110 99 140 180

70 100 67 160 160

60 100 62 130 150

53 39 53 130 120

64 26 68 160 160

82 130 90 240 170

1.4 1.5

1.3 1.4 1 U 0.97

1 U 0.8 U 

1 U 1 U 1.2 U 1 U 0.8 U 

1 U 1 U 1.2 U 1 U 0.8 U 

1.2 U 1 U 0.8 U 

83 60 140 83 280

85 110 140 110 290

78 74 110 87 200

99 170 84 180 200

93 110 82 180 190

61 32 67 94 140

68 26 71 92 180

77 34 84 110 250

59 18 68 84 170

64 86 59 92 120

93 120 82 130 170

76 34 69 120 170

71 120 95 110 210

58 91 73 92 180

95 20 140 150 270

110 150 110 220 330

84 86 91 160 240

110 150 140 230 370

1.2 U 1 U 0.8 U 

1 U 1 U 1.2 U 1 U 0.8 U 

1 U 1 U 1.2 U 1 U 0.8 U 

1 U 1 U 1.2 U 1 U 0.8 U 

1 U 1 U 1.2 U 1 U 0.8 U 

1.2 U 1 U 1.3

11 15 11 48 37
Table A3-4. Continued

Species Age Class Station Sample Location

White Sucker Adult 603389 9408 Rochester Park

White Sucker Adult 603389 9409 Rochester Park

White Sucker Young of year 603389 9410 Rochester Park

White Sucker Young of year 603389 9411 Rochester Park

White Sucker Young of year 603389 9412 Rochester Park

White Sucker Adult 603390 9407 Esselingen Park

White Sucker Adult 603390 9408 Esselingen Park

White Sucker Adult 603390 9409 Esselingen Park

White Sucker Young of year 603390 9410 Esselingen Park

White Sucker Young of year 603390 9411 Esselingen Park

White Sucker Young of year 603390 9412 Esselingen Park

White Sucker Adult 603391 9407 Between Kohler Dams

White Sucker Adult 603391 9408 Between Kohler Dams

White Sucker Adult 603391 9409 Between Kohler Dams

White Sucker Young of year 603391 9410 Between Kohler Dams

White Sucker Young of year 603391 9411 Between Kohler Dams

White Sucker Young of year 603391 9412 Between Kohler Dams

PCB018 PCB019 PCB022 PCB024/027 PCB026

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

16 10 27 14 33

50 79 38 99

56 35 67 45 83

49 56 53 70 86

64 21 65 77 100

14 8.8 13 24 33

48 58 59 70 100

15 21 20 25 36

25 24 25 32 59

17 17 19 29 48

27 39 35 50 70

16 23 47 47

18 18 21 37 40

49 57 59 100 130

40 44 39 83 93

72 90 79 170 200

40 36 57 84 110
Table A3-4. Continued

Species Age Class Station Sample Location

Crayfish 603384 9409 Control

Crayfish 603384 9410 Control

Crayfish 603384 9411 Control

Crayfish 603385 9409 Rochester Park

Crayfish 603385 9410 Rochester Park

Crayfish 603385 9411 Rochester Park

Crayfish 603386 9409 Between Kohler Dams

Crayfish 603386 9410 Between Kohler Dams

Crayfish 603386 9411 Between Kohler Dams

Crayfish 603387 9409 Esselingen Park

Crayfish 603387 9410 Esselingen Park

Crayfish 603387 9411 Esselingen Park

Emergent Insects 603384 9401 Control

Emergent Insects 603384 9402 Control

Emergent Insects 603384 9403 Control

Emergent Insects 603384 9404 Control

Emergent Insects 603385 9401 Rochester Park

Emergent Insects 603385 9402 Rochester Park

Emergent Insects 603385 9403 Rochester Park

Emergent Insects 603385 9404 Rochester Park

Emergent Insects 603386 9401 Between Kohler Dams

Emergent Insects 603386 9402 Between Kohler Dams

Emergent Insects 603386 9403 Between Kohler Dams

Emergent Insects 603386 9404 Between Kohler Dams

Emergent Insects 603387 9401 Esselingen Park

Emergent Insects 603387 9402 Esselingen Park

Emergent Insects 603387 9403 Esselingen Park

Emergent Insects 603387 9404 Esselingen Park

Larval Insects 603384 9405 Control

Larval Insects 603384 9406 Control

Larval Insects 603384 9407 Control

Larval Insects 603384 9408 Control

Larval Insects 603385 9405 Rochester Park

Larval Insects 603385 9406 Rochester Park

Larval Insects 603385 9407 Rochester Park

Larval Insects 603385 9408 Rochester Park

Larval Insects 603386 9405 Between Kohler Dams

Larval Insects 603386 9406 Between Kohler Dams

Larval Insects 603386 9407 Between Kohler Dams

Larval Insects 603386 9408 Between Kohler Dams

Larval Insects 603387 9406 Esselingen Park

Larval Insects 603387 9407 Esselingen Park

Larval Insects 603387 9408 Esselingen Park

PCB031/028 PCB033 PCB037/042 PCB040 PCB041/071/006

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

2.5 U 1 U 1.1 U 1 U 2 U 

2.5 U 1 U 1.1 U 1 U 2 U 

3.8 1 U 1.1 U 1 U 2 U 

250 4.5 15 44

250 4.6 16 45

250 5.7 U*I 16 1.6 47

400 5 U*I 18 1.6 52

320 6 U*I 20 1.9 58

310 5.8 U*I 19 1.5 57

190 2 U*I 12 1 U 36

210 2 U*I 13 1 U 39

230 1.3 U*I 13 46

8 1 U 3 1 U 5.3

4.5 U*I 1 U 1.1 U 1 U 2 U 

2.5 U 1 U 1.1 U 1 U 2 U 

3.5 U*I 1 U 1.1 U 1 U 2 U 

880 85 U*I 290 61 560

1500 61 U*I 490 100 1000

800 77 U*I 270 52 540

840 36 U*I 310 58 580

1200 81 U*I 360 66 730

1200 73 U*I 310 52 640

540 12 U*I 140 25 300

630 91 U*I 180 37 370

780 80 U*I 140 29 330

520 67 U*I 130 22 270

700 62 U*I 160 25 340

660 93 U*I 190 24 380

2.5 U 1 U 1.1 U 1 U 2 U 

4.7 1 U 1.1 U 1 U 2 U 

2.5 U 1 U 1.1 U 1 U 2 U 

3.3 U*I 1 U 1.1 U 1 U 2 U 

790 58 U*I 190 43 420

640 64 U*I 130 26 330

760 58 U*I 190 45 410

770 50 U*I 140 40 340

820 130 U*I 170 37 370

820 54 U*I 150 30 350

750 64 U*I 140 32 320

760 62 U*I 120 30 310

470 37 U*I 100 24 230

620 33 U*I 110 24 240

610 34 U*I 110 23 250
Table A3-4. Continued

Species Age Class Station Sample Location

Longnose Dace Young of year 603388 9413 Control

Longnose Dace Young of year 603388 9414 Control

Longnose Dace Young of year 603388 9415 Control

Longnose Dace Young of year 603389 9413 Rochester Park

Longnose Dace Young of year 603389 9414 Rochester Park

Longnose Dace Young of year 603389 9415 Rochester Park

Longnose Dace Young of year 603390 9413 Esselingen Park

Longnose Dace Young of year 603390 9414 Esselingen Park

Longnose Dace Young of year 603390 9415 Esselingen Park

Longnose Dace Young of year 603391 9413 Between Kohler Dams

Longnose Dace Young of year 603391 9414 Between Kohler Dams

Longnose Dace Young of year 603391 9415 Between Kohler Dams

Smallmouth Bass Adult 603388 9401 Control

Smallmouth Bass Adult 603388 9402 Control

Smallmouth Bass Adult 603388 9403 Control

Smallmouth Bass Young of year 603388 9404 Control

Smallmouth Bass Young of year 603388 9405 Control

Smallmouth Bass Young of year 603388 9406 Control

Smallmouth Bass Adult 603389 9401 Rochester Park

Smallmouth Bass Adult 603389 9402 Rochester Park

Smallmouth Bass Adult 603389 9403 Rochester Park

Smallmouth Bass Young of year 603389 9404 Rochester Park

Smallmouth Bass Young of year 603389 9405 Rochester Park

Smallmouth Bass Young of year 603389 9406 Rochester Park

Smallmouth Bass Adult 603390 9401 Esselingen Park

Smallmouth Bass Adult 603390 9402 Esselingen Park

Smallmouth Bass Adult 603390 9403 Esselingen Park

Smallmouth Bass Young of year 603390 9404 Esselingen Park

Smallmouth Bass Young of year 603390 9405 Esselingen Park

Smallmouth Bass Young of year 603390 9406 Esselingen Park

Smallmouth Bass Adult 603391 9401 Between Kohler Dams

Smallmouth Bass Adult 603391 9402 Between Kohler Dams

Smallmouth Bass Adult 603391 9403 Between Kohler Dams

Smallmouth Bass Young of year 603391 9404 Between Kohler Dams

Smallmouth Bass Young of year 603391 9405 Between Kohler Dams

Smallmouth Bass Young of year 603391 9406 Between Kohler Dams

White Sucker Adult 603388 9407 Control

White Sucker Adult 603388 9408 Control

White Sucker Adult 603388 9409 Control

White Sucker Young of year 603388 9410 Control

White Sucker Young of year 603388 9411 Control

White Sucker Young of year 603388 9412 Control

White Sucker Adult 603389 9407 Rochester Park

PCB031/028 PCB033 PCB037/042 PCB040 PCB041/071/006

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

19 3 U*I 4.8 1.2 12

2.5 U 1 U 1.1 U 1 U 2 U 

2.5 U 1 U 1.1 U 1 U 2 U 

720 35 U*I 100 41 330

790 40 U*I 120 47 390

1400 100 U*I 350 74 800

1500 160 U*I 380 96 940

1400 170 U*I 350 84 890

1100 140 U*I 260 66 650

1200 120 U*I 250 42 600

1600 160 U*I 320 46 790

1700 190 U*I 380 85 890

15 1 U 7.2 1.7 19

9.1 1.6 1.8 1 U 3.9

3.2 2 U 

2.5 U 1 U 1.1 U 1 U 2 U 

2.5 U 1 U 1.1 U 1 U 2 U 

5 1.1 U 2.8

1900 130 U*I 430 130 1400

2000 120 U*I 360 120 1300

1500 90 U*I 220 75 820

1500 180 U*I 320 76 720

1500 99 U*I 210 74 720

1200 80 U*I 300 52 630

1300 120 U*I 270 42 700

1500 120 U*I 300 44 770

1300 89 U*I 230 32 610

860 65 U*I 130 50 430

1200 55 U*I 180 71 670

1300 130 U*I 280 46 640

1400 74 U*I 250 52 670

1100 64 U*I 200 48 540

1800 130 U*I 540 72 1100

1800 260 U*I 390 82 960

1400 190 U*I 310 66 780

1800 160 U*I 530 110 1200

5.1 1.1 U 2.6

2.5 U 1 U 1.1 U 1 U 2 U 

2.5 U 1 U 1.1 U 1 U 2 U 

2.5 U 1 U 1.1 U 1 U 2 U 

2.5 U 1 U 1.1 U 1 U 2 U 

13 U*I 1 U 2.4 5.4

410 39 U*I 85 9.4 210
Table A3-4. Continued

Species Age Class Station Sample Location

White Sucker Adult 603389 9408 Rochester Park

White Sucker Adult 603389 9409 Rochester Park

White Sucker Young of year 603389 9410 Rochester Park

White Sucker Young of year 603389 9411 Rochester Park

White Sucker Young of year 603389 9412 Rochester Park

White Sucker Adult 603390 9407 Esselingen Park

White Sucker Adult 603390 9408 Esselingen Park

White Sucker Adult 603390 9409 Esselingen Park

White Sucker Young of year 603390 9410 Esselingen Park

White Sucker Young of year 603390 9411 Esselingen Park

White Sucker Young of year 603390 9412 Esselingen Park

White Sucker Adult 603391 9407 Between Kohler Dams

White Sucker Adult 603391 9408 Between Kohler Dams

White Sucker Adult 603391 9409 Between Kohler Dams

White Sucker Young of year 603391 9410 Between Kohler Dams

White Sucker Young of year 603391 9411 Between Kohler Dams

White Sucker Young of year 603391 9412 Between Kohler Dams

PCB031/028 PCB033 PCB037/042 PCB040 PCB041/071/006

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

370 23 U*I 120 29 310

1200 70 U*I 270 74 960

690 60 U*I 160 50 520

860 50 U*I 140 43 450

880 85 U*I 220 41 540

230 54 8 140

1000 35 U*I 170 45 700

390 14 U*I 69 19 260

380 46 U*I 79 17 200

330 40 U*I 76 13 170

470 57 U*I 110 24 260

430 58 U*I 140 13 330

540 63 U*I 150 23 390

920 120 U*I 210 43 530

710 92 U*I 160 28 360

1400 180 U*I 300 56 720

860 60 U*I 180 32 370
Table A3-4. Continued

Species Age Class Station Sample Location

Crayfish 603384 9409 Control

Crayfish 603384 9410 Control

Crayfish 603384 9411 Control

Crayfish 603385 9409 Rochester Park

Crayfish 603385 9410 Rochester Park

Crayfish 603385 9411 Rochester Park

Crayfish 603386 9409 Between Kohler Dams

Crayfish 603386 9410 Between Kohler Dams

Crayfish 603386 9411 Between Kohler Dams

Crayfish 603387 9409 Esselingen Park

Crayfish 603387 9410 Esselingen Park

Crayfish 603387 9411 Esselingen Park

Emergent Insects 603384 9401 Control

Emergent Insects 603384 9402 Control

Emergent Insects 603384 9403 Control

Emergent Insects 603384 9404 Control

Emergent Insects 603385 9401 Rochester Park

Emergent Insects 603385 9402 Rochester Park

Emergent Insects 603385 9403 Rochester Park

Emergent Insects 603385 9404 Rochester Park

Emergent Insects 603386 9401 Between Kohler Dams

Emergent Insects 603386 9402 Between Kohler Dams

Emergent Insects 603386 9403 Between Kohler Dams

Emergent Insects 603386 9404 Between Kohler Dams

Emergent Insects 603387 9401 Esselingen Park

Emergent Insects 603387 9402 Esselingen Park

Emergent Insects 603387 9403 Esselingen Park

Emergent Insects 603387 9404 Esselingen Park

Larval Insects 603384 9405 Control

Larval Insects 603384 9406 Control

Larval Insects 603384 9407 Control

Larval Insects 603384 9408 Control

Larval Insects 603385 9405 Rochester Park

Larval Insects 603385 9406 Rochester Park

Larval Insects 603385 9407 Rochester Park

Larval Insects 603385 9408 Rochester Park

Larval Insects 603386 9405 Between Kohler Dams

Larval Insects 603386 9406 Between Kohler Dams

Larval Insects 603386 9407 Between Kohler Dams

Larval Insects 603386 9408 Between Kohler Dams

Larval Insects 603387 9406 Esselingen Park

Larval Insects 603387 9407 Esselingen Park

Larval Insects 603387 9408 Esselingen Park

PCB044 PCB045 PCB046 PCB047/048 PCB049

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

1.1 U 0.8 U 1 U 1.2 U 0.6 U 

1.1 U 0.8 U 1 U 1.2 U 0.6 U 

1.2 0.8 U 1 U 4.5 2

12 240 86

11 230 83

12 250 85

11 410 110

13 390 110

12 1 U 330 110

8 0.8 U 1 U 210 72

8 0.8 U 1 U 240 81

8.8 270 93

5.2 0.8 U 1 U 6.4 4.6

1.2 0.8 U 1 U 3.1 1.5

1.1 U 0.8 U 1 U 1.2 U 0.6 U 

1.5 0.8 U 1 U 3.1 1.7

460 45 19 940 540

800 77 30 1400 810

420 39 17 920 440

440 42 20 1000 470

520 61 25 1900 700

450 48 18 1600 600

210 23 8 640 280

260 34 13 760 330

200 26 6.6 670 280

180 18 6.3 600 280

200 23 7.5 760 340

240 21 7 750 340

1.1 U 0.8 U 1 U 1.2 U 0.6 U 

1.3 0.8 U 1 U 4 2

1.1 U 0.8 U 1 U 1.2 U 0.6 U 

1.1 U 0.8 U 1 U 1.2 U 0.6 U 

320 32 14 660 340

200 17 6.7 430 250

300 31 14 640 320

190 23 9.4 610 280

250 37 14 730 310

210 27 9.1 740 320

180 28 10 640 280

120 21 6.8 630 280

130 18 6.7 410 190

120 16 6 480 240

120 16 5.4 470 230
Table A3-4. Continued

Species Age Class Station Sample Location

Longnose Dace Young of year 603388 9413 Control

Longnose Dace Young of year 603388 9414 Control

Longnose Dace Young of year 603388 9415 Control

Longnose Dace Young of year 603389 9413 Rochester Park

Longnose Dace Young of year 603389 9414 Rochester Park

Longnose Dace Young of year 603389 9415 Rochester Park

Longnose Dace Young of year 603390 9413 Esselingen Park

Longnose Dace Young of year 603390 9414 Esselingen Park

Longnose Dace Young of year 603390 9415 Esselingen Park

Longnose Dace Young of year 603391 9413 Between Kohler Dams

Longnose Dace Young of year 603391 9414 Between Kohler Dams

Longnose Dace Young of year 603391 9415 Between Kohler Dams

Smallmouth Bass Adult 603388 9401 Control

Smallmouth Bass Adult 603388 9402 Control

Smallmouth Bass Adult 603388 9403 Control

Smallmouth Bass Young of year 603388 9404 Control

Smallmouth Bass Young of year 603388 9405 Control

Smallmouth Bass Young of year 603388 9406 Control

Smallmouth Bass Adult 603389 9401 Rochester Park

Smallmouth Bass Adult 603389 9402 Rochester Park

Smallmouth Bass Adult 603389 9403 Rochester Park

Smallmouth Bass Young of year 603389 9404 Rochester Park

Smallmouth Bass Young of year 603389 9405 Rochester Park

Smallmouth Bass Young of year 603389 9406 Rochester Park

Smallmouth Bass Adult 603390 9401 Esselingen Park

Smallmouth Bass Adult 603390 9402 Esselingen Park

Smallmouth Bass Adult 603390 9403 Esselingen Park

Smallmouth Bass Young of year 603390 9404 Esselingen Park

Smallmouth Bass Young of year 603390 9405 Esselingen Park

Smallmouth Bass Young of year 603390 9406 Esselingen Park

Smallmouth Bass Adult 603391 9401 Between Kohler Dams

Smallmouth Bass Adult 603391 9402 Between Kohler Dams

Smallmouth Bass Adult 603391 9403 Between Kohler Dams

Smallmouth Bass Young of year 603391 9404 Between Kohler Dams

Smallmouth Bass Young of year 603391 9405 Between Kohler Dams

Smallmouth Bass Young of year 603391 9406 Between Kohler Dams

White Sucker Adult 603388 9407 Control

White Sucker Adult 603388 9408 Control

White Sucker Adult 603388 9409 Control

White Sucker Young of year 603388 9410 Control

White Sucker Young of year 603388 9411 Control

White Sucker Young of year 603388 9412 Control

White Sucker Adult 603389 9407 Rochester Park

PCB044 PCB045 PCB046 PCB047/048 PCB049

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

8.4 0.92 22 12

1.1 U 0.8 U 1 U 1.2 U 0.7

1.1 U 0.8 U 1 U 1.2 U 0.6

260 40 8.2 870 340

290 45 8.3 1000 390

620 63 9 1400 760

700 80 12 1700 910

640 82 11 1700 850

470 66 10 1300 630

420 46 7.6 1500 610

520 50 8 1900 790

660 86 17 2200 920

13 0.93 22 15

3.3 0.8 U 1 U 6 3.7

1.3 3.7 2.1

1.1 U 0.8 U 1 U 1.2 U 0.6 U 

1.1 U 0.8 U 1 U 1.2 U 0.6 U 

1.9 0.8 U 1 U 5.3 2.9

940 82 27 2400 1300

790 75 23 2500 1200

520 55 16 1800 850

480 84 23 1900 750

490 72 20 1300 590

480 43 12 1400 680

460 44 11 1600 740

490 46 13 1700 780

380 33 8.1 1500 700

310 50 15 1200 460

490 71 22 1600 630

460 47 12 1500 680

390 53 17 2100 810

350 46 14 1500 600

710 62 18 2600 1100

610 97 27 2200 890

460 71 19 1700 680

840 97 34 2700 1200

1.7 4.9 2.7

1.1 U 0.8 U 1 U 1.2 U 0.6 U 

1.1 U 0.8 U 1 U 1.2 U 0.6 U 

1.1 U 0.8 U 1 U 1.2 U 0.6 U 

1.1 U 0.8 U 1 U 1.2 U 0.6 U 

3.8 0.8 U 13 7.4

110 11 600 190
Table A3-4. Continued

Species Age Class Station Sample Location

White Sucker Adult 603389 9408 Rochester Park

White Sucker Adult 603389 9409 Rochester Park

White Sucker Young of year 603389 9410 Rochester Park

White Sucker Young of year 603389 9411 Rochester Park

White Sucker Young of year 603389 9412 Rochester Park

White Sucker Adult 603390 9407 Esselingen Park

White Sucker Adult 603390 9408 Esselingen Park

White Sucker Adult 603390 9409 Esselingen Park

White Sucker Young of year 603390 9410 Esselingen Park

White Sucker Young of year 603390 9411 Esselingen Park

White Sucker Young of year 603390 9412 Esselingen Park

White Sucker Adult 603391 9407 Between Kohler Dams

White Sucker Adult 603391 9408 Between Kohler Dams

White Sucker Adult 603391 9409 Between Kohler Dams

White Sucker Young of year 603391 9410 Between Kohler Dams

White Sucker Young of year 603391 9411 Between Kohler Dams

White Sucker Young of year 603391 9412 Between Kohler Dams

PCB044 PCB045 PCB046 PCB047/048 PCB049

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

210 17 6.8 660 260

600 51 19 1600 730

350 45 14 900 380

300 42 11 1200 410

330 40 12 1200 400

84 7.9 290 130

450 45 12 1500 620

150 15 450 220

130 16 4.5 410 190

120 13 3 420 180

160 22 6.5 580 250

170 14 860 330

230 21 6.1 880 360

350 42 14 1100 480

250 35 8.2 920 360

470 72 14 1900 680

240 35 8.1 1100 410
Table A3-4. Continued

Species Age Class Station Sample Location

Crayfish 603384 9409 Control

Crayfish 603384 9410 Control

Crayfish 603384 9411 Control

Crayfish 603385 9409 Rochester Park

Crayfish 603385 9410 Rochester Park

Crayfish 603385 9411 Rochester Park

Crayfish 603386 9409 Between Kohler Dams

Crayfish 603386 9410 Between Kohler Dams

Crayfish 603386 9411 Between Kohler Dams

Crayfish 603387 9409 Esselingen Park

Crayfish 603387 9410 Esselingen Park

Crayfish 603387 9411 Esselingen Park

Emergent Insects 603384 9401 Control

Emergent Insects 603384 9402 Control

Emergent Insects 603384 9403 Control

Emergent Insects 603384 9404 Control

Emergent Insects 603385 9401 Rochester Park

Emergent Insects 603385 9402 Rochester Park

Emergent Insects 603385 9403 Rochester Park

Emergent Insects 603385 9404 Rochester Park

Emergent Insects 603386 9401 Between Kohler Dams

Emergent Insects 603386 9402 Between Kohler Dams

Emergent Insects 603386 9403 Between Kohler Dams

Emergent Insects 603386 9404 Between Kohler Dams

Emergent Insects 603387 9401 Esselingen Park

Emergent Insects 603387 9402 Esselingen Park

Emergent Insects 603387 9403 Esselingen Park

Emergent Insects 603387 9404 Esselingen Park

Larval Insects 603384 9405 Control

Larval Insects 603384 9406 Control

Larval Insects 603384 9407 Control

Larval Insects 603384 9408 Control

Larval Insects 603385 9405 Rochester Park

Larval Insects 603385 9406 Rochester Park

Larval Insects 603385 9407 Rochester Park

Larval Insects 603385 9408 Rochester Park

Larval Insects 603386 9405 Between Kohler Dams

Larval Insects 603386 9406 Between Kohler Dams

Larval Insects 603386 9407 Between Kohler Dams

Larval Insects 603386 9408 Between Kohler Dams

Larval Insects 603387 9406 Esselingen Park

Larval Insects 603387 9407 Esselingen Park

Larval Insects 603387 9408 Esselingen Park

PCB052 PCB056/060 PCB066/095 PCB070/076 PCB074

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

1 U 1.8 U 2.8 U 2.4 U 1 U 

1 U 1.8 U 2.8 U 2.4 U 1 U 

2.2 1.8 U 2.8 U 2.4 U 1.1

85 64 200 59 78

83 55 180 58 69

82 56 170 54 68

98 67 200 53 84

100 67 200 58 79

99 62 190 55 75

68 54 150 41 60

76 64 180 46 69

87 59 170 47 68

6.6 5.3 14 20 U*I 6.6

2.4 1.8 U 7.5 U*I 8 U*I 4 U*I

1 U 1.8 U 2.8 U 2.4 U 1.5 U*I

2.2 1.8 U 4 3.3 2 U*I

650 390 1100 890 400

1000 680 1700 1400 610

530 400 870 740 340

570 410 890 790 360

790 360 980 810 400

660 310 940 680 340

300 170 440 330 170

370 200 490 360 190

330 230 550 360 220

320 180 540 370 170

370 210 630 410 190

370 250 590 410 220

1 U 1.8 U 2.8 U 2.4 U 4 U*I

2.3 1.8 U 3.3 2.4 U 3 U*I

1 U 1.8 U 2.8 U 2.4 U 3.5 U*I

1 U 1.8 U 2.8 U 2.4 U 4 U*I

400 310 710 560 240

290 230 580 470 210

380 310 730 580 250

320 250 660 510 220

340 200 500 360 200

350 190 480 340 190

310 170 420 310 170

310 160 400 300 170

210 150 350 240 140

260 160 400 240 140

250 160 370 250 140
Table A3-4. Continued

Species Age Class Station Sample Location

Longnose Dace Young of year 603388 9413 Control

Longnose Dace Young of year 603388 9414 Control

Longnose Dace Young of year 603388 9415 Control

Longnose Dace Young of year 603389 9413 Rochester Park

Longnose Dace Young of year 603389 9414 Rochester Park

Longnose Dace Young of year 603389 9415 Rochester Park

Longnose Dace Young of year 603390 9413 Esselingen Park

Longnose Dace Young of year 603390 9414 Esselingen Park

Longnose Dace Young of year 603390 9415 Esselingen Park

Longnose Dace Young of year 603391 9413 Between Kohler Dams

Longnose Dace Young of year 603391 9414 Between Kohler Dams

Longnose Dace Young of year 603391 9415 Between Kohler Dams

Smallmouth Bass Adult 603388 9401 Control

Smallmouth Bass Adult 603388 9402 Control

Smallmouth Bass Adult 603388 9403 Control

Smallmouth Bass Young of year 603388 9404 Control

Smallmouth Bass Young of year 603388 9405 Control

Smallmouth Bass Young of year 603388 9406 Control

Smallmouth Bass Adult 603389 9401 Rochester Park

Smallmouth Bass Adult 603389 9402 Rochester Park

Smallmouth Bass Adult 603389 9403 Rochester Park

Smallmouth Bass Young of year 603389 9404 Rochester Park

Smallmouth Bass Young of year 603389 9405 Rochester Park

Smallmouth Bass Young of year 603389 9406 Rochester Park

Smallmouth Bass Adult 603390 9401 Esselingen Park

Smallmouth Bass Adult 603390 9402 Esselingen Park

Smallmouth Bass Adult 603390 9403 Esselingen Park

Smallmouth Bass Young of year 603390 9404 Esselingen Park

Smallmouth Bass Young of year 603390 9405 Esselingen Park

Smallmouth Bass Young of year 603390 9406 Esselingen Park

Smallmouth Bass Adult 603391 9401 Between Kohler Dams

Smallmouth Bass Adult 603391 9402 Between Kohler Dams

Smallmouth Bass Adult 603391 9403 Between Kohler Dams

Smallmouth Bass Young of year 603391 9404 Between Kohler Dams

Smallmouth Bass Young of year 603391 9405 Between Kohler Dams

Smallmouth Bass Young of year 603391 9406 Between Kohler Dams

White Sucker Adult 603388 9407 Control

White Sucker Adult 603388 9408 Control

White Sucker Adult 603388 9409 Control

White Sucker Young of year 603388 9410 Control

White Sucker Young of year 603388 9411 Control

White Sucker Young of year 603388 9412 Control

White Sucker Adult 603389 9407 Rochester Park

PCB052 PCB056/060 PCB066/095 PCB070/076 PCB074

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

14 9.5 33 18 13

1.4 1.8 U 6.4 4.6 2.9

1 U 1.8 U 3.1 2.6 1.6

390 160 470 260 180

440 180 560 300 210

930 400 1500 740 530

1000 430 1100 530 480

980 370 1200 490 470

720 260 830 410 320

670 200 780 360 290

880 250 1000 480 380

1000 310 1300 510 390

16 18 55 31 17

4.8 3.9 11 6 3.2

2.7 1.8 U 6.3 2.8 1.8

1 U 1.8 U 2.8 U 2.4 U 1 U 

1 U 1.8 U 2.8 U 2.4 U 1 U 

3.5 2.1 7.4 4.2 2.7

1300 920 3300 1700 1100

1300 980 2900 1500 1000

910 640 1900 900 660

640 430 1500 800 520

770 420 1200 670 410

750 420 1400 810 520

840 380 1200 530 460

850 430 1300 580 510

780 370 1200 430 480

500 200 540 290 210

700 280 870 400 280

800 270 910 490 350

820 450 1400 470 560

620 330 890 410 340

1200 690 2000 760 740

950 450 1400 710 500

690 410 1200 590 450

1200 600 1800 900 670

3 2.1 7.1 3.9 2.5

1 U 1.8 U 2.8 U 2.4 U 1 U 

1 U 1.8 U 2.8 U 2.4 U 1 U 

1 U 1.8 U 2.8 U 2.4 U 1 U 

1 U 1.8 U 2.8 U 2.4 U 1 U 

5.9 4.5 15 7.9 5.6

100 110 420 190 160
Table A3-4. Continued

Species Age Class Station Sample Location

White Sucker Adult 603389 9408 Rochester Park

White Sucker Adult 603389 9409 Rochester Park

White Sucker Young of year 603389 9410 Rochester Park

White Sucker Young of year 603389 9411 Rochester Park

White Sucker Young of year 603389 9412 Rochester Park

White Sucker Adult 603390 9407 Esselingen Park

White Sucker Adult 603390 9408 Esselingen Park

White Sucker Adult 603390 9409 Esselingen Park

White Sucker Young of year 603390 9410 Esselingen Park

White Sucker Young of year 603390 9411 Esselingen Park

White Sucker Young of year 603390 9412 Esselingen Park

White Sucker Adult 603391 9407 Between Kohler Dams

White Sucker Adult 603391 9408 Between Kohler Dams

White Sucker Adult 603391 9409 Between Kohler Dams

White Sucker Young of year 603391 9410 Between Kohler Dams

White Sucker Young of year 603391 9411 Between Kohler Dams

White Sucker Young of year 603391 9412 Between Kohler Dams

PCB052 PCB056/060 PCB066/095 PCB070/076 PCB074

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

170 220 690 330 240

560 430 1400 800 520

300 280 790 460 270

300 240 750 380 250

320 210 670 370 260

130 66 220 110 87

650 310 1100 530 400

190 130 400 180 160

150 99 280 160 100

140 83 260 130 91

180 140 380 200 140

280 160 520 210 200

270 200 690 290 270

330 290 840 470 330

320 170 510 250 180

560 330 950 480 350

320 190 540 290 200
Table A3-4. Continued

Species Age Class Station Sample Location

Crayfish 603384 9409 Control

Crayfish 603384 9410 Control

Crayfish 603384 9411 Control

Crayfish 603385 9409 Rochester Park

Crayfish 603385 9410 Rochester Park

Crayfish 603385 9411 Rochester Park

Crayfish 603386 9409 Between Kohler Dams

Crayfish 603386 9410 Between Kohler Dams

Crayfish 603386 9411 Between Kohler Dams

Crayfish 603387 9409 Esselingen Park

Crayfish 603387 9410 Esselingen Park

Crayfish 603387 9411 Esselingen Park

Emergent Insects 603384 9401 Control

Emergent Insects 603384 9402 Control

Emergent Insects 603384 9403 Control

Emergent Insects 603384 9404 Control

Emergent Insects 603385 9401 Rochester Park

Emergent Insects 603385 9402 Rochester Park

Emergent Insects 603385 9403 Rochester Park

Emergent Insects 603385 9404 Rochester Park

Emergent Insects 603386 9401 Between Kohler Dams

Emergent Insects 603386 9402 Between Kohler Dams

Emergent Insects 603386 9403 Between Kohler Dams

Emergent Insects 603386 9404 Between Kohler Dams

Emergent Insects 603387 9401 Esselingen Park

Emergent Insects 603387 9402 Esselingen Park

Emergent Insects 603387 9403 Esselingen Park

Emergent Insects 603387 9404 Esselingen Park

Larval Insects 603384 9405 Control

Larval Insects 603384 9406 Control

Larval Insects 603384 9407 Control

Larval Insects 603384 9408 Control

Larval Insects 603385 9405 Rochester Park

Larval Insects 603385 9406 Rochester Park

Larval Insects 603385 9407 Rochester Park

Larval Insects 603385 9408 Rochester Park

Larval Insects 603386 9405 Between Kohler Dams

Larval Insects 603386 9406 Between Kohler Dams

Larval Insects 603386 9407 Between Kohler Dams

Larval Insects 603386 9408 Between Kohler Dams

Larval Insects 603387 9406 Esselingen Park

Larval Insects 603387 9407 Esselingen Park

Larval Insects 603387 9408 Esselingen Park

PCB077 PCB077/110 PCB082 PCB084/092 PCB085

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

1 U 1.3 U 1 U 1.3 U 1 U 

1 U 1.3 U 1 U 1.3 U 1 U 

2.7 1 U 1.3 U 1 U 

5.2 54 4.6 7.9 30

4.4 48 3.3 24

55 4.7 7.9 29

2.5 *Q 64 4.9 7.9 30

1.9 *Q 69 5.7 10 29

67 4.8 7.7 26

2.3 51 4 6.1 24

2.8 57 4.5 6.3 28

64 4.8 6.4 27

1 U 12 1.2 4.7 1.3 U*I

1 U 6.1 1 U 1.6 1.5 U*I

1 U 3.7 1 U 1.3 U 1 U 

1 U 5.6 1 U 1.5 1.5 U*I

30 910 85 390 150

49 1300 120 670 240

32 770 77 290 120

32 820 87 330 140

31 1100 86 450 140

25 900 66 320 100

12 *Q 480 40 160 57

12 *Q 510 43 210 68

13 *Q 430 38 150 69

10 *Q 540 41 150 68

12 *Q 630 47 170 77

12 610 55 200 91

1 U 1.6 1 U 1.3 U 1 U 

3.8 1 U 1.3 U 1 U 

1 U 1.3 U 1 U 1.3 U 1 U 

1 U 1.4 1 U 1.3 U 1 U 

23 470 51 200 93

380 38 130 80

22 470 55 200 98

20 400 46 150 80

10 *Q 410 40 180 57

400 35 160 54

7.5 *Q 360 31 150 48

8.6 *Q 370 32 140 50

5.9 300 28 120 44

7.8 320 30 120 46

5.8 320 29 120 47
Table A3-4. Continued

Species Age Class Station Sample Location

Longnose Dace Young of year 603388 9413 Control

Longnose Dace Young of year 603388 9414 Control

Longnose Dace Young of year 603388 9415 Control

Longnose Dace Young of year 603389 9413 Rochester Park

Longnose Dace Young of year 603389 9414 Rochester Park

Longnose Dace Young of year 603389 9415 Rochester Park

Longnose Dace Young of year 603390 9413 Esselingen Park

Longnose Dace Young of year 603390 9414 Esselingen Park

Longnose Dace Young of year 603390 9415 Esselingen Park

Longnose Dace Young of year 603391 9413 Between Kohler Dams

Longnose Dace Young of year 603391 9414 Between Kohler Dams

Longnose Dace Young of year 603391 9415 Between Kohler Dams

Smallmouth Bass Adult 603388 9401 Control

Smallmouth Bass Adult 603388 9402 Control

Smallmouth Bass Adult 603388 9403 Control

Smallmouth Bass Young of year 603388 9404 Control

Smallmouth Bass Young of year 603388 9405 Control

Smallmouth Bass Young of year 603388 9406 Control

Smallmouth Bass Adult 603389 9401 Rochester Park

Smallmouth Bass Adult 603389 9402 Rochester Park

Smallmouth Bass Adult 603389 9403 Rochester Park

Smallmouth Bass Young of year 603389 9404 Rochester Park

Smallmouth Bass Young of year 603389 9405 Rochester Park

Smallmouth Bass Young of year 603389 9406 Rochester Park

Smallmouth Bass Adult 603390 9401 Esselingen Park

Smallmouth Bass Adult 603390 9402 Esselingen Park

Smallmouth Bass Adult 603390 9403 Esselingen Park

Smallmouth Bass Young of year 603390 9404 Esselingen Park

Smallmouth Bass Young of year 603390 9405 Esselingen Park

Smallmouth Bass Young of year 603390 9406 Esselingen Park

Smallmouth Bass Adult 603391 9401 Between Kohler Dams

Smallmouth Bass Adult 603391 9402 Between Kohler Dams

Smallmouth Bass Adult 603391 9403 Between Kohler Dams

Smallmouth Bass Young of year 603391 9404 Between Kohler Dams

Smallmouth Bass Young of year 603391 9405 Between Kohler Dams

Smallmouth Bass Young of year 603391 9406 Between Kohler Dams

White Sucker Adult 603388 9407 Control

White Sucker Adult 603388 9408 Control

White Sucker Adult 603388 9409 Control

White Sucker Young of year 603388 9410 Control

White Sucker Young of year 603388 9411 Control

White Sucker Young of year 603388 9412 Control

White Sucker Adult 603389 9407 Rochester Park

PCB077 PCB077/110 PCB082 PCB084/092 PCB085

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

1 U 29 2.5 7.2 7.5 U*I

8.1 1 U 1.5 1.8 U*I

3.9 1 U 1.3 U 1 U

470 38 190 79

600 43 210 94

16 1200 98 350 180

20 1300 120 500 170

1300 110 480 160 U*I

920 72 360 120 U*I

830 60 300 100

1000 70 300 130

17 1200 89 400 120

1.2 45 4.8 12 6.3

1 U 13 1 U 2.4 3 U*I

1 U 8 1.4 2.6 U*I

3.8 1 U 1.3 U 1 U

4.5 1 U 1.3 U 1 U

1 U 8.6 1 U 1.7 2 U*I

98 2700 240 580 560

86 2400 210 590 530

48 1400 120 380 320

1200 99 390 220

1100 130 390 220

29 1200 100 310 220

17 1100 73 280 160

16 1200 82 300 170

13 1000 65 220 170

670 44 240 80

900 59 330 110

14 940 60 280 110

36 1100 90 250 220

26 830 68 230 150

42 1700 160 420 330

1400 100 540 200

1100 92 420 170

41 2000 170 760 280

1 U 8.3 1 U 1.8 2 U*I

2 1 U 1.3 U 1 U 

1.3 U 1 U 1.3 U 1 U 

1.6 1 U 1.3 U 1 U 

2.1 1 U 1.3 U 1 U 

1 U 12 1 3 2 U*I

4 440 34 100 99
Table A3-4. Continued

Species Age Class Station Sample Location

White Sucker Adult 603389 9408 Rochester Park

White Sucker Adult 603389 9409 Rochester Park

White Sucker Young of year 603389 9410 Rochester Park

White Sucker Young of year 603389 9411 Rochester Park

White Sucker Young of year 603389 9412 Rochester Park

White Sucker Adult 603390 9407 Esselingen Park

White Sucker Adult 603390 9408 Esselingen Park

White Sucker Adult 603390 9409 Esselingen Park

White Sucker Young of year 603390 9410 Esselingen Park

White Sucker Young of year 603390 9411 Esselingen Park

White Sucker Young of year 603390 9412 Esselingen Park

White Sucker Adult 603391 9407 Between Kohler Dams

White Sucker Adult 603391 9408 Between Kohler Dams

White Sucker Adult 603391 9409 Between Kohler Dams

White Sucker Young of year 603391 9410 Between Kohler Dams

White Sucker Young of year 603391 9411 Between Kohler Dams

White Sucker Young of year 603391 9412 Between Kohler Dams

PCB077 PCB077/110 PCB082 PCB084/092 PCB085

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

530 57 160 140

1100 89 360 210

640 55 250 130

660 52 240 130

11 590 43 200 100

2.3 250 16 61 32

1100 69 290 140

400 28 120 58

260 20 95 34 U*I

240 20 90 35 U*I

6.4 330 29 130 51

5.6 480 40 140 83

650 51 190 68 U*I

800 64 270 78 U*I

470 35 200 72

860 66 390 130

7.8 450 38 180 78
Table A3-4. Continued

Species Age Class Station Sample Location

Crayfish 603384 9409 Control

Crayfish 603384 9410 Control

Crayfish 603384 9411 Control

Crayfish 603385 9409 Rochester Park

Crayfish 603385 9410 Rochester Park

Crayfish 603385 9411 Rochester Park

Crayfish 603386 9409 Between Kohler Dams

Crayfish 603386 9410 Between Kohler Dams

Crayfish 603386 9411 Between Kohler Dams

Crayfish 603387 9409 Esselingen Park

Crayfish 603387 9410 Esselingen Park

Crayfish 603387 9411 Esselingen Park

Emergent Insects 603384 9401 Control

Emergent Insects 603384 9402 Control

Emergent Insects 603384 9403 Control

Emergent Insects 603384 9404 Control

Emergent Insects 603385 9401 Rochester Park

Emergent Insects 603385 9402 Rochester Park

Emergent Insects 603385 9403 Rochester Park

Emergent Insects 603385 9404 Rochester Park

Emergent Insects 603386 9401 Between Kohler Dams

Emergent Insects 603386 9402 Between Kohler Dams

Emergent Insects 603386 9403 Between Kohler Dams

Emergent Insects 603386 9404 Between Kohler Dams

Emergent Insects 603387 9401 Esselingen Park

Emergent Insects 603387 9402 Esselingen Park

Emergent Insects 603387 9403 Esselingen Park

Emergent Insects 603387 9404 Esselingen Park

Larval Insects 603384 9405 Control

Larval Insects 603384 9406 Control

Larval Insects 603384 9407 Control

Larval Insects 603384 9408 Control

Larval Insects 603385 9405 Rochester Park

Larval Insects 603385 9406 Rochester Park

Larval Insects 603385 9407 Rochester Park

Larval Insects 603385 9408 Rochester Park

Larval Insects 603386 9405 Between Kohler Dams

Larval Insects 603386 9406 Between Kohler Dams

Larval Insects 603386 9407 Between Kohler Dams

Larval Insects 603386 9408 Between Kohler Dams

Larval Insects 603387 9406 Esselingen Park

Larval Insects 603387 9407 Esselingen Park

Larval Insects 603387 9408 Esselingen Park

PCB087 PCB091 PCB097 PCB099 PCB101

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

1 U 1 U 0.8 U 0.6 U 0.6

1 U 1 U 0.8 U 0.6 U 0.63

1 U 1 U 0.8 U 1.1 1.7

37 18 25 66 87

30 16 22 58 75

33 17 25 60 78

38 26 30 76 120

36 26 30 71 94

36 22 29 69 94

32 19 23 56 75

37 20 27 64 85

38 19 28 66 92

3.7 2.4 3 4.2 7.2

1.9 1.2 1.4 2.8 4.8

1.1 1 U 0.8 U 1.6 2.8

1.6 1 U 1.3 2.3 3.7

260 210 230 330 510

400 330 360 500 740

250 160 170 290 430

260 190 220 310 460

270 280 260 390 580

190 220 170 320 470

110 130 100 160 240

120 150 110 180 260

110 130 100 180 250

120 130 110 170 280

130 160 120 190 330

160 170 140 220 300

1 U 1 U 0.8 U 0.65 1.1

1 U 1 U 0.8 U 1.5 2.3

1 U 1 U 0.8 U 0.6 U 0.61

1 U 1 U 0.8 U 0.62 0.86

160 120 120 170 260

120 82 95 140 200

160 120 130 180 260

130 94 97 150 220

100 130 99 160 210

100 110 94 150 220

89 110 78 130 180

85 110 71 130 190

79 83 65 100 140

80 88 65 110 150

83 91 67 110 150
Table A3-4. Continued

Species Age Class Station Sample Location

Longnose Dace Young of year 603388 9413 Control

Longnose Dace Young of year 603388 9414 Control

Longnose Dace Young of year 603388 9415 Control

Longnose Dace Young of year 603389 9413 Rochester Park

Longnose Dace Young of year 603389 9414 Rochester Park

Longnose Dace Young of year 603389 9415 Rochester Park

Longnose Dace Young of year 603390 9413 Esselingen Park

Longnose Dace Young of year 603390 9414 Esselingen Park

Longnose Dace Young of year 603390 9415 Esselingen Park

Longnose Dace Young of year 603391 9413 Between Kohler Dams

Longnose Dace Young of year 603391 9414 Between Kohler Dams

Longnose Dace Young of year 603391 9415 Between Kohler Dams

Smallmouth Bass Adult 603388 9401 Control

Smallmouth Bass Adult 603388 9402 Control

Smallmouth Bass Adult 603388 9403 Control

Smallmouth Bass Young of year 603388 9404 Control

Smallmouth Bass Young of year 603388 9405 Control

Smallmouth Bass Young of year 603388 9406 Control

Smallmouth Bass Adult 603389 9401 Rochester Park

Smallmouth Bass Adult 603389 9402 Rochester Park

Smallmouth Bass Adult 603389 9403 Rochester Park

Smallmouth Bass Young of year 603389 9404 Rochester Park

Smallmouth Bass Young of year 603389 9405 Rochester Park

Smallmouth Bass Young of year 603389 9406 Rochester Park

Smallmouth Bass Adult 603390 9401 Esselingen Park

Smallmouth Bass Adult 603390 9402 Esselingen Park

Smallmouth Bass Adult 603390 9403 Esselingen Park

Smallmouth Bass Young of year 603390 9404 Esselingen Park

Smallmouth Bass Young of year 603390 9405 Esselingen Park

Smallmouth Bass Young of year 603390 9406 Esselingen Park

Smallmouth Bass Adult 603391 9401 Between Kohler Dams

Smallmouth Bass Adult 603391 9402 Between Kohler Dams

Smallmouth Bass Adult 603391 9403 Between Kohler Dams

Smallmouth Bass Young of year 603391 9404 Between Kohler Dams

Smallmouth Bass Young of year 603391 9405 Between Kohler Dams

Smallmouth Bass Young of year 603391 9406 Between Kohler Dams

White Sucker Adult 603388 9407 Control

White Sucker Adult 603388 9408 Control

White Sucker Adult 603388 9409 Control

White Sucker Young of year 603388 9410 Control

White Sucker Young of year 603388 9411 Control

White Sucker Young of year 603388 9412 Control

White Sucker Adult 603389 9407 Rochester Park

PCB087 PCB091 PCB097 PCB099 PCB101

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

10 6.1 8.1 14 22

2.7 1 1.8 3.3 6.7

1.3 1 U 0.85 1.6 3.1

140 130 120 180 310

160 150 140 200 350

370 220 300 440 700

360 300 300 450 760

350 380 300 450 740

240 290 210 320 520

200 200 160 320 490

250 250 210 400 610

290 290 260 450 700

17 8.8 13 20 29

4.4 1.8 3 6 10

3 1.2 2 4.2 7

1.1 1 U 0.81 1.7 2.8

1.5 1 U 0.96 2.1 3.5

3 1.4 2.2 4.2 6.8

780 470 650 1200 1700

720 520 600 1100 1500

440 330 360 620 920

290 270 250 510 690

340 240 270 410 640

320 220 260 510 710

330 220 260 470 680

350 280 280 530 730

330 210 260 520 740

150 180 130 200 360

210 240 180 310 470

230 210 170 280 520

390 250 330 730 990

260 200 200 440 620

590 460 490 890 1200

330 410 320 540 750

270 330 270 460 590

560 560 500 840 1200

2.7 1.4 2 3.9 5.9

1 U 1 U 0.8 U 0.91 1.2

1 U 1 U 0.8 U 0.6 U 0.72

1 U 1 U 0.8 U 0.67 0.93

1 U 1 U 0.8 U 0.88 1.4

4 2.6 3.2 5.7 8.2

130 100 120 200 230
Table A3-4. Continued

Species Age Class Station Sample Location

White Sucker Adult 603389 9408 Rochester Park

White Sucker Adult 603389 9409 Rochester Park

White Sucker Young of year 603389 9410 Rochester Park

White Sucker Young of year 603389 9411 Rochester Park

White Sucker Young of year 603389 9412 Rochester Park

White Sucker Adult 603390 9407 Esselingen Park

White Sucker Adult 603390 9408 Esselingen Park

White Sucker Adult 603390 9409 Esselingen Park

White Sucker Young of year 603390 9410 Esselingen Park

White Sucker Young of year 603390 9411 Esselingen Park

White Sucker Young of year 603390 9412 Esselingen Park

White Sucker Adult 603391 9407 Between Kohler Dams

White Sucker Adult 603391 9408 Between Kohler Dams

White Sucker Adult 603391 9409 Between Kohler Dams

White Sucker Young of year 603391 9410 Between Kohler Dams

White Sucker Young of year 603391 9411 Between Kohler Dams

White Sucker Young of year 603391 9412 Between Kohler Dams

PCB087 PCB091 PCB097 PCB099 PCB101

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

190 130 170 270 350

300 260 270 450 620

190 170 160 230 330

190 180 160 240 370

150 150 140 220 290

63 52 54 96 140

270 240 220 420 600

110 100 93 150 210

68 72 56 100 140

66 77 59 98 140

88 93 78 130 190

120 140 120 230 310

180 180 170 290 390

210 220 200 350 470

110 160 110 190 280

210 330 200 340 480

120 140 110 180 290
Table A3-4. Continued

Species Age Class Station Sample Location

Crayfish 603384 9409 Control

Crayfish 603384 9410 Control

Crayfish 603384 9411 Control

Crayfish 603385 9409 Rochester Park

Crayfish 603385 9410 Rochester Park

Crayfish 603385 9411 Rochester Park

Crayfish 603386 9409 Between Kohler Dams

Crayfish 603386 9410 Between Kohler Dams

Crayfish 603386 9411 Between Kohler Dams

Crayfish 603387 9409 Esselingen Park

Crayfish 603387 9410 Esselingen Park

Crayfish 603387 9411 Esselingen Park

Emergent Insects 603384 9401 Control

Emergent Insects 603384 9402 Control

Emergent Insects 603384 9403 Control

Emergent Insects 603384 9404 Control

Emergent Insects 603385 9401 Rochester Park

Emergent Insects 603385 9402 Rochester Park

Emergent Insects 603385 9403 Rochester Park

Emergent Insects 603385 9404 Rochester Park

Emergent Insects 603386 9401 Between Kohler Dams

Emergent Insects 603386 9402 Between Kohler Dams

Emergent Insects 603386 9403 Between Kohler Dams

Emergent Insects 603386 9404 Between Kohler Dams

Emergent Insects 603387 9401 Esselingen Park

Emergent Insects 603387 9402 Esselingen Park

Emergent Insects 603387 9403 Esselingen Park

Emergent Insects 603387 9404 Esselingen Park

Larval Insects 603384 9405 Control

Larval Insects 603384 9406 Control

Larval Insects 603384 9407 Control

Larval Insects 603384 9408 Control

Larval Insects 603385 9405 Rochester Park

Larval Insects 603385 9406 Rochester Park

Larval Insects 603385 9407 Rochester Park

Larval Insects 603385 9408 Rochester Park

Larval Insects 603386 9405 Between Kohler Dams

Larval Insects 603386 9406 Between Kohler Dams

Larval Insects 603386 9407 Between Kohler Dams

Larval Insects 603386 9408 Between Kohler Dams

Larval Insects 603387 9406 Esselingen Park

Larval Insects 603387 9407 Esselingen Park

Larval Insects 603387 9408 Esselingen Park

PCB105 PCB118 PCB123 PCB126 PCB128

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

1 U 0.8 U 1 U 1 U 1.4 U 

1 U 0.8 U 1 U 1 U 1.4 U 

1.5 1.4 U 

29 98 8 U*I 1 U 10

22 83 2.1 U*I 1 U 7.9

79 10

26 130 7.1 U*I 1 U 12

21 95 8.7 U*I 1 U 11

88 12

19 80 4 U*I 1 U 9.4

23 87 4 U*I 1 U 11

83 12

2.8 9.3 1 U 1 U 1.5

1.6 6 1 U 1 U 1.4 U 

1 U 3.6 1 U 1 U 1.4 U 

1.4 4.9 1 U 1 U 1.4 U 

290 710 19 U*I 1 U 73

260 820 15 U*I 1 U 79

170 450 12 U*I 1 U 54

170 480 19 U*I 1 U 62

160 600 28 U*I 1 U 72

100 480 21 U*I 1 U 53

87 260 17 U*I 1 U 31

71 260 20 U*I 1 U 32

81 270 15 U*I 1 U 31

80 310 16 U*I 1 U 35

97 340 19 U*I 1 U 40

130 390 13 U*I 1 U 46

1 U 1.6 1 U 1 U 1.4 U 

2.6 1.4 U 

1 U 1.2 1 U 1 U 1.4 U 

1 U 1.7 1 U 1 U 1.4 U 

100 290 9.3 U*I 1 U 34

210 26

110 290 9 U*I 1 U 36

88 250 7.6 U*I 1 U 28

81 230 17 U*I 1 U 27

230 27

62 180 11 U*I 1 U 21

63 180 13 U*I 1 U 20

51 170 5.8 U*I 1 U 19

50 170 7 U*I 1 U 21

43 180 7.4 U*I 1 U 21
Table A3-4. Continued

Species Age Class Station Sample Location

Longnose Dace Young of year 603388 9413 Control

Longnose Dace Young of year 603388 9414 Control

Longnose Dace Young of year 603388 9415 Control

Longnose Dace Young of year 603389 9413 Rochester Park

Longnose Dace Young of year 603389 9414 Rochester Park

Longnose Dace Young of year 603389 9415 Rochester Park

Longnose Dace Young of year 603390 9413 Esselingen Park

Longnose Dace Young of year 603390 9414 Esselingen Park

Longnose Dace Young of year 603390 9415 Esselingen Park

Longnose Dace Young of year 603391 9413 Between Kohler Dams

Longnose Dace Young of year 603391 9414 Between Kohler Dams

Longnose Dace Young of year 603391 9415 Between Kohler Dams

Smallmouth Bass Adult 603388 9401 Control

Smallmouth Bass Adult 603388 9402 Control

Smallmouth Bass Adult 603388 9403 Control

Smallmouth Bass Young of year 603388 9404 Control

Smallmouth Bass Young of year 603388 9405 Control

Smallmouth Bass Young of year 603388 9406 Control

Smallmouth Bass Adult 603389 9401 Rochester Park

Smallmouth Bass Adult 603389 9402 Rochester Park

Smallmouth Bass Adult 603389 9403 Rochester Park

Smallmouth Bass Young of year 603389 9404 Rochester Park

Smallmouth Bass Young of year 603389 9405 Rochester Park

Smallmouth Bass Young of year 603389 9406 Rochester Park

Smallmouth Bass Adult 603390 9401 Esselingen Park

Smallmouth Bass Adult 603390 9402 Esselingen Park

Smallmouth Bass Adult 603390 9403 Esselingen Park

Smallmouth Bass Young of year 603390 9404 Esselingen Park

Smallmouth Bass Young of year 603390 9405 Esselingen Park

Smallmouth Bass Young of year 603390 9406 Esselingen Park

Smallmouth Bass Adult 603391 9401 Between Kohler Dams

Smallmouth Bass Adult 603391 9402 Between Kohler Dams

Smallmouth Bass Adult 603391 9403 Between Kohler Dams

Smallmouth Bass Young of year 603391 9404 Between Kohler Dams

Smallmouth Bass Young of year 603391 9405 Between Kohler Dams

Smallmouth Bass Young of year 603391 9406 Between Kohler Dams

White Sucker Adult 603388 9407 Control

White Sucker Adult 603388 9408 Control

White Sucker Adult 603388 9409 Control

White Sucker Young of year 603388 9410 Control

White Sucker Young of year 603388 9411 Control

White Sucker Young of year 603388 9412 Control

White Sucker Adult 603389 9407 Rochester Park

PCB105 PCB118 PCB123 PCB126 PCB128

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

9.9 25 1 U 1 U 3.5

6.8 1.6

3.3 1.4 U 

300 34

320 36

240 740 17 U*I 2 U*I 71

260 760 20 U*I 1 U 87

700 78

460 53

460 54

560 63

170 560 16 U*I 1 U 65

8.6 39 1.2 U*I 1 U 5.5

4.5 13 1 U 1 U 2.5

3.1 8.1 1 U 1 U 1.7

3.9 1.4 U 

4.4 1.4 U 

1.4 7.6 1 U 1 U 1.4 U 

770 2000 110 U*I 5.4 260

920 1900 120 U*I 4 240

460 1100 75 U*I 1.8 130

920 90

720 110

340 920 23 U*I 2.4 U*I 93

210 720 16 U*I 1.2 U*I 73

250 820 18 U*I 1.4 U*I 93

240 820 17 U*I 1.5 U*I 92

250 32

360 45

170 480 12 U*I 1 U 49

340 1200 25 U*I 2.2 140

210 680 18 U*I 1.2 88

460 1400 44 U*I 2.4 170

830 100

720 88

430 1200 37 U*I 2 160

2.8 7.8 1 U 1 U 1.4 U 

2.1 1.4 U 

1.2 1.4 U 

1.3 1.4 U 

1.6 1.4 U 

3.2 11 1 U 1 U 1.6

130 320 8.8 U*I 1.2 U*I 48
Table A3-4. Continued

Species Age Class Station Sample Location

White Sucker Adult 603389 9408 Rochester Park

White Sucker Adult 603389 9409 Rochester Park

White Sucker Young of year 603389 9410 Rochester Park

White Sucker Young of year 603389 9411 Rochester Park

White Sucker Young of year 603389 9412 Rochester Park

White Sucker Adult 603390 9407 Esselingen Park

White Sucker Adult 603390 9408 Esselingen Park

White Sucker Adult 603390 9409 Esselingen Park

White Sucker Young of year 603390 9410 Esselingen Park

White Sucker Young of year 603390 9411 Esselingen Park

White Sucker Young of year 603390 9412 Esselingen Park

White Sucker Adult 603391 9407 Between Kohler Dams

White Sucker Adult 603391 9408 Between Kohler Dams

White Sucker Adult 603391 9409 Between Kohler Dams

White Sucker Young of year 603391 9410 Between Kohler Dams

White Sucker Young of year 603391 9411 Between Kohler Dams

White Sucker Young of year 603391 9412 Between Kohler Dams

PCB105 PCB118 PCB123 PCB126 PCB128

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

470 57

730 82

370 50

400 50

130 310 9 U*I 1.9 U*I 41

40 140 4.2 U*I 1 U 17

550 68

200 28

140 22

140 20

61 200 5.6 U*I 1 U 25

120 330 8.1 U*I 1 U 42

470 59

540 61

250 36

450 59

83 260 7.1 U*I 1 U 31
Table A3-4. Continued

Species Age Class Station Sample Location

Crayfish 603384 9409 Control

Crayfish 603384 9410 Control

Crayfish 603384 9411 Control

Crayfish 603385 9409 Rochester Park

Crayfish 603385 9410 Rochester Park

Crayfish 603385 9411 Rochester Park

Crayfish 603386 9409 Between Kohler Dams

Crayfish 603386 9410 Between Kohler Dams

Crayfish 603386 9411 Between Kohler Dams

Crayfish 603387 9409 Esselingen Park

Crayfish 603387 9410 Esselingen Park

Crayfish 603387 9411 Esselingen Park

Emergent Insects 603384 9401 Control

Emergent Insects 603384 9402 Control

Emergent Insects 603384 9403 Control

Emergent Insects 603384 9404 Control

Emergent Insects 603385 9401 Rochester Park

Emergent Insects 603385 9402 Rochester Park

Emergent Insects 603385 9403 Rochester Park

Emergent Insects 603385 9404 Rochester Park

Emergent Insects 603386 9401 Between Kohler Dams

Emergent Insects 603386 9402 Between Kohler Dams

Emergent Insects 603386 9403 Between Kohler Dams

Emergent Insects 603386 9404 Between Kohler Dams

Emergent Insects 603387 9401 Esselingen Park

Emergent Insects 603387 9402 Esselingen Park

Emergent Insects 603387 9403 Esselingen Park

Emergent Insects 603387 9404 Esselingen Park

Larval Insects 603384 9405 Control

Larval Insects 603384 9406 Control

Larval Insects 603384 9407 Control

Larval Insects 603384 9408 Control

Larval Insects 603385 9405 Rochester Park

Larval Insects 603385 9406 Rochester Park

Larval Insects 603385 9407 Rochester Park

Larval Insects 603385 9408 Rochester Park

Larval Insects 603386 9405 Between Kohler Dams

Larval Insects 603386 9406 Between Kohler Dams

Larval Insects 603386 9407 Between Kohler Dams

Larval Insects 603386 9408 Between Kohler Dams

Larval Insects 603387 9406 Esselingen Park

Larval Insects 603387 9407 Esselingen Park

Larval Insects 603387 9408 Esselingen Park

PCB132/153 PCB136 PCB137/176 PCB138/163 PCB141

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

1.8 U 2 U 1 U 1.7 U 0.8 U 

1.8 U 2 U 1 U 1.7 U 0.8 U 

1.9 2 U 1 U 1.7 U 0.8 U 

63 65 12 U*I

55 55 10 U*I

58 1 U 64 9 U*I

78 10 U*I 1 U 84 14 U*I

75 2 U*I 1 U 81 13 U*I

69 2 U 1 U 76 9.9 U*I

58 2 U 5.2 66 9 U*I

65 2 U 1 U 73 10 U*I

70 2 U 1 U 82 11 U*I

11 2 U 1 U 9.9 1.3 U*I

8.2 2 U 1 U 8.4 1 U*I

7 2 U 1 U 6.4 0.8 U

7.8 2 U 1 U 7.4 0.8 U

540 17 U*I 2.1 U*I 550 68 U*I

590 21 2.3 U*I 610 83 U*I

350 13 1.6 U*I 390 56 U*I

390 15 2 U*I 430 63 U*I

530 19 2.8 U*I 530 69 U*I

420 14 2 U*I 420 51 U*I

240 14 U*I 240 35 U*I

250 17 U*I 240 36 U*I

230 10 U*I 230 31 U*I

240 12 U*I 260 35 U*I

260 15 U*I 290 42 U*I

290 18 U*I 320 40 U*I

2.7 2 U 1 U 2.5 0.8 U

3.6 2 U 1 U 3.2 0.8 U

2 2 U 1 U 1.9 0.8 U

2.7 2 U 1 U 2.6 0.8 U

190 9.3 1 U 210 34 U*I

140 5.6 1 U 160 22 U*I

190 9.4 1 U 220 36 U*I

140 5.8 1 U 180 28 U*I

190 13 U*I 190 27 U*I

190 12 U*I 190 22 U*I

150 11 U*I 150 21 U*I

150 9 U*I 150 30 U*I

120 9 U*I 130 17 U*I

120 9 U*I 1 U 130 20 U*I

120 9 U*I 1 U 140 19 U*I
Table A3-4. Continued

Species Age Class Station Sample Location

Longnose Dace Young of year 603388 9413 Control

Longnose Dace Young of year 603388 9414 Control

Longnose Dace Young of year 603388 9415 Control

Longnose Dace Young of year 603389 9413 Rochester Park

Longnose Dace Young of year 603389 9414 Rochester Park

Longnose Dace Young of year 603389 9415 Rochester Park

Longnose Dace Young of year 603390 9413 Esselingen Park

Longnose Dace Young of year 603390 9414 Esselingen Park

Longnose Dace Young of year 603390 9415 Esselingen Park

Longnose Dace Young of year 603391 9413 Between Kohler Dams

Longnose Dace Young of year 603391 9414 Between Kohler Dams

Longnose Dace Young of year 603391 9415 Between Kohler Dams

Smallmouth Bass Adult 603388 9401 Control

Smallmouth Bass Adult 603388 9402 Control

Smallmouth Bass Adult 603388 9403 Control

Smallmouth Bass Young of year 603388 9404 Control

Smallmouth Bass Young of year 603388 9405 Control

Smallmouth Bass Young of year 603388 9406 Control

Smallmouth Bass Adult 603389 9401 Rochester Park

Smallmouth Bass Adult 603389 9402 Rochester Park

Smallmouth Bass Adult 603389 9403 Rochester Park

Smallmouth Bass Young of year 603389 9404 Rochester Park

Smallmouth Bass Young of year 603389 9405 Rochester Park

Smallmouth Bass Young of year 603389 9406 Rochester Park

Smallmouth Bass Adult 603390 9401 Esselingen Park

Smallmouth Bass Adult 603390 9402 Esselingen Park

Smallmouth Bass Adult 603390 9403 Esselingen Park

Smallmouth Bass Young of year 603390 9404 Esselingen Park

Smallmouth Bass Young of year 603390 9405 Esselingen Park

Smallmouth Bass Young of year 603390 9406 Esselingen Park

Smallmouth Bass Adult 603391 9401 Between Kohler Dams

Smallmouth Bass Adult 603391 9402 Between Kohler Dams

Smallmouth Bass Adult 603391 9403 Between Kohler Dams

Smallmouth Bass Young of year 603391 9404 Between Kohler Dams

Smallmouth Bass Young of year 603391 9405 Between Kohler Dams

Smallmouth Bass Young of year 603391 9406 Between Kohler Dams

White Sucker Adult 603388 9407 Control

White Sucker Adult 603388 9408 Control

White Sucker Adult 603388 9409 Control

White Sucker Young of year 603388 9410 Control

White Sucker Young of year 603388 9411 Control

White Sucker Young of year 603388 9412 Control

White Sucker Adult 603389 9407 Rochester Park

PCB132/153 PCB136 PCB137/176 PCB138/163 PCB141

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

26 2 U 1 U 25 3 U*I

14 2 U 1 U 12 1.7 U*I

7.4 2 U 1 U 6.3 0.8 U

230 5.7 20 U*I 240 35 U*I

260 6.6 20 U*I 260 40 U*I

440 14 490 69 U*I

760 15 U*I 2.5 U*I 690 81 U*I

650 12 U*I 620 70 U*I

470 9.5 U*I 1.3 420 50 U*I

460 19 U*I 410 50 U*I

560 24 U*I 1.6 U*I 500 60 U*I

670 14 U*I 2.5 U*I 530 64 U*I

31 2 U 1 U 34 4 U*I

21 2 U 1 U 18 2 U*I

13 2 U 1 U 11 1.3 U*I

6.7 2 U 1 U 5.6 0.8 U

8.3 2 U 1 U 6.8 0.8 U

10 2 U 1 U 9.5 1.2 U*I

1400 44 5 U*I 1600 200 U*I

1400 33 4 U*I 1600 190 U*I

770 17 2 U*I 830 110 U*I

570 26 U*I 1.4 630 75 U*I

580 18 1.3 720 110 U*I

580 19 630 81 U*I

510 22 U*I 560 65

620 26 U*I 680 78 U*I

660 21 U*I 690 82 U*I

240 20 U*I 20 U*I 240 32 U*I

380 11 25 U*I 360 45 U*I

400 13 390 47 U*I

1100 28 U*I 3.5 U*I 1000 130 U*I

730 12 U*I 2.3 U*I 660 83 U*I

1300 24 U*I 3.7 U*I 1200 160 U*I

690 26 2.1 690 75 U*I

550 30 U*I 1.3 580 64 U*I

1300 35 U*I 4.5 U*I 1200 150 U*I

10 2 U 1 U 9.5 1 U*I

4.8 2 U 1 U 4 0.8 U

3.5 2 U 1 U 2.8 0.8 U

3.3 2 U 1 U 2.7 0.8 U

3.7 2 U 1 U 3.3 0.8 U

9.2 2 U 1 U 9.6 1.2 U*I

280 7.3 300 31 U*I
Table A3-4. Continued

Species Age Class Station Sample Location

White Sucker Adult 603389 9408 Rochester Park

White Sucker Adult 603389 9409 Rochester Park

White Sucker Young of year 603389 9410 Rochester Park

White Sucker Young of year 603389 9411 Rochester Park

White Sucker Young of year 603389 9412 Rochester Park

White Sucker Adult 603390 9407 Esselingen Park

White Sucker Adult 603390 9408 Esselingen Park

White Sucker Adult 603390 9409 Esselingen Park

White Sucker Young of year 603390 9410 Esselingen Park

White Sucker Young of year 603390 9411 Esselingen Park

White Sucker Young of year 603390 9412 Esselingen Park

White Sucker Adult 603391 9407 Between Kohler Dams

White Sucker Adult 603391 9408 Between Kohler Dams

White Sucker Adult 603391 9409 Between Kohler Dams

White Sucker Young of year 603391 9410 Between Kohler Dams

White Sucker Young of year 603391 9411 Between Kohler Dams

White Sucker Young of year 603391 9412 Between Kohler Dams

PCB132/153 PCB136 PCB137/176 PCB138/163 PCB141

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

410 8.4 25 410 44 U*I

530 14 600 70 U*I

260 11 290 40 U*I

340 12 22 310 41 U*I

250 10 270 33 U*I

130 130 15 U*I

520 35 U*I 540 65 U*I

200 15 U*I 200 24 U*I

140 7.7 U*I 130 11 U*I

140 17 U*I 130 12

170 18 U*I 12 U*I 170 19 U*I

350 6.5 U*I 1.9 U*I 270 31 U*I

400 87 U*I 400 36 U*I

460 90 U*I 1.2 460 45 U*I

250 16 U*I 230 22 U*I

420 31 U*I 1.7 390 40 U*I

230 7.5 U*I 15 U*I 220 26 U*I
Table A3-4. Continued

Species Age Class Station Sample Location

Crayfish 603384 9409 Control

Crayfish 603384 9410 Control

Crayfish 603384 9411 Control

Crayfish 603385 9409 Rochester Park

Crayfish 603385 9410 Rochester Park

Crayfish 603385 9411 Rochester Park

Crayfish 603386 9409 Between Kohler Dams

Crayfish 603386 9410 Between Kohler Dams

Crayfish 603386 9411 Between Kohler Dams

Crayfish 603387 9409 Esselingen Park

Crayfish 603387 9410 Esselingen Park

Crayfish 603387 9411 Esselingen Park

Emergent Insects 603384 9401 Control

Emergent Insects 603384 9402 Control

Emergent Insects 603384 9403 Control

Emergent Insects 603384 9404 Control

Emergent Insects 603385 9401 Rochester Park

Emergent Insects 603385 9402 Rochester Park

Emergent Insects 603385 9403 Rochester Park

Emergent Insects 603385 9404 Rochester Park

Emergent Insects 603386 9401 Between Kohler Dams

Emergent Insects 603386 9402 Between Kohler Dams

Emergent Insects 603386 9403 Between Kohler Dams

Emergent Insects 603386 9404 Between Kohler Dams

Emergent Insects 603387 9401 Esselingen Park

Emergent Insects 603387 9402 Esselingen Park

Emergent Insects 603387 9403 Esselingen Park

Emergent Insects 603387 9404 Esselingen Park

Larval Insects 603384 9405 Control

Larval Insects 603384 9406 Control

Larval Insects 603384 9407 Control

Larval Insects 603384 9408 Control

Larval Insects 603385 9405 Rochester Park

Larval Insects 603385 9406 Rochester Park

Larval Insects 603385 9407 Rochester Park

Larval Insects 603385 9408 Rochester Park

Larval Insects 603386 9405 Between Kohler Dams

Larval Insects 603386 9406 Between Kohler Dams

Larval Insects 603386 9407 Between Kohler Dams

Larval Insects 603386 9408 Between Kohler Dams

Larval Insects 603387 9406 Esselingen Park

Larval Insects 603387 9407 Esselingen Park

Larval Insects 603387 9408 Esselingen Park

PCB144/135 PCB146 PCB149 PCB151 PCB156

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

0.8 U 1 U 1.1 U 1 U 1 U 

0.8 U 1 U 1.1 U 1 U 1 U 

0.8 U 1 U 1.1 U 1 U 

2.9 17 24 5.1 6.3

2.4 15 20 4.5 5.1

3 17 23 5.6

4.1 27 32 7.7 5.8

4.3 25 33 8 6.3

3.9 23 30 7.2

3.9 19 26 6.9 3.9

3.4 20 27 6.1 4.7

3.9 23 32 7.1

0.8 U 1.7 4.1 1 U 1 U 

0.8 U 1.3 2.6 1 U 1 U 

0.8 U 1.1 2 1 U 1 U 

0.8 U 1.2 2.5 1 U 1 U 

48 86 280 18 55

59 100 300 27 57

38 70 160 18 38

45 80 210 15 44

71 120 300 21 46

52 90 240 21 34

33 65 130 18 20

34 60 130 15 20

23 55 110 27 16

29 61 130 17 18

37 71 160 19 23

44 75 180 12 18

0.8 U 1 U 1.1 U 1 U 1 U 

0.8 U 1 U 1.3 1 U 

0.8 U 1 U 1.1 U 1 U 1 U 

0.8 U 1 U 1.1 U 1 U 1 U 

21 38 90 20 22

16 28 69 13

22 39 92 18 21

16 30 72 15 15

26 48 100 24 13

25 45 99 19

23 39 85 20 10

24 38 84 23 10

18 30 68 13 7.4

19 31 67 16 7.8

20 32 70 17 6.2
Table A3-4. Continued

Species Age Class Station Sample Location

Longnose Dace Young of year 603388 9413 Control

Longnose Dace Young of year 603388 9414 Control

Longnose Dace Young of year 603388 9415 Control

Longnose Dace Young of year 603389 9413 Rochester Park

Longnose Dace Young of year 603389 9414 Rochester Park

Longnose Dace Young of year 603389 9415 Rochester Park

Longnose Dace Young of year 603390 9413 Esselingen Park

Longnose Dace Young of year 603390 9414 Esselingen Park

Longnose Dace Young of year 603390 9415 Esselingen Park

Longnose Dace Young of year 603391 9413 Between Kohler Dams

Longnose Dace Young of year 603391 9414 Between Kohler Dams

Longnose Dace Young of year 603391 9415 Between Kohler Dams

Smallmouth Bass Adult 603388 9401 Control

Smallmouth Bass Adult 603388 9402 Control

Smallmouth Bass Adult 603388 9403 Control

Smallmouth Bass Young of year 603388 9404 Control

Smallmouth Bass Young of year 603388 9405 Control

Smallmouth Bass Young of year 603388 9406 Control

Smallmouth Bass Adult 603389 9401 Rochester Park

Smallmouth Bass Adult 603389 9402 Rochester Park

Smallmouth Bass Adult 603389 9403 Rochester Park

Smallmouth Bass Young of year 603389 9404 Rochester Park

Smallmouth Bass Young of year 603389 9405 Rochester Park

Smallmouth Bass Young of year 603389 9406 Rochester Park

Smallmouth Bass Adult 603390 9401 Esselingen Park

Smallmouth Bass Adult 603390 9402 Esselingen Park

Smallmouth Bass Adult 603390 9403 Esselingen Park

Smallmouth Bass Young of year 603390 9404 Esselingen Park

Smallmouth Bass Young of year 603390 9405 Esselingen Park

Smallmouth Bass Young of year 603390 9406 Esselingen Park

Smallmouth Bass Adult 603391 9401 Between Kohler Dams

Smallmouth Bass Adult 603391 9402 Between Kohler Dams

Smallmouth Bass Adult 603391 9403 Between Kohler Dams

Smallmouth Bass Young of year 603391 9404 Between Kohler Dams

Smallmouth Bass Young of year 603391 9405 Between Kohler Dams

Smallmouth Bass Young of year 603391 9406 Between Kohler Dams

White Sucker Adult 603388 9407 Control

White Sucker Adult 603388 9408 Control

White Sucker Adult 603388 9409 Control

White Sucker Young of year 603388 9410 Control

White Sucker Young of year 603388 9411 Control

White Sucker Young of year 603388 9412 Control

White Sucker Adult 603389 9407 Rochester Park

PCB144/135 PCB146 PCB149 PCB151 PCB156

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

1.6 4.8 9.4 2.3 2.4

0.8 U 2.1 3.9 1 U 

0.8 U 1 1.8 1 U 

24 49 120 24

26 54 140 28

47 83 210 44 44

71 140 400 69 54

71 140 330 67

53 100 250 52

48 99 200 45

61 120 300 56

65 130 360 59 38

1.9 5.4 12 2.6 1.6

0.89 2.9 5.8 1.3 1.4

0.8 U 2 3.7 1 U 1.1

0.8 U 1 U 1.6 1 U 

0.8 U 1.1 2 1 U 

0.8 U 1.6 3.2 1 U 1 U 

97 280 600 150 170

100 280 590 160 190

60 150 350 81 90

57 100 240 67

62 140 310 83

41 100 200 58 54

44 110 260 56 41

53 130 310 71 53

46 140 290 62 51

29 53 130 33

41 74 190 46

39 76 170 47 23

64 240 490 95 88

50 150 340 71 64

85 240 600 120 120

85 150 380 91

63 130 270 69

100 230 660 130 99

0.8 U 1.6 2.8 1 U 1 U 

0.8 U 1 U 1.1 U 1 U 

0.8 U 1 U 1.1 U 1 U 

0.8 U 1 U 1.1 U 1 U 

0.8 U 1 U 1.1 U 1 U 

0.8 U 1.7 3.2 1 U 1 U 

17 60 99 23 27
Table A3-4. Continued

Species Age Class Station Sample Location

White Sucker Adult 603389 9408 Rochester Park

White Sucker Adult 603389 9409 Rochester Park

White Sucker Young of year 603389 9410 Rochester Park

White Sucker Young of year 603389 9411 Rochester Park

White Sucker Young of year 603389 9412 Rochester Park

White Sucker Adult 603390 9407 Esselingen Park

White Sucker Adult 603390 9408 Esselingen Park

White Sucker Adult 603390 9409 Esselingen Park

White Sucker Young of year 603390 9410 Esselingen Park

White Sucker Young of year 603390 9411 Esselingen Park

White Sucker Young of year 603390 9412 Esselingen Park

White Sucker Adult 603391 9407 Between Kohler Dams

White Sucker Adult 603391 9408 Between Kohler Dams

White Sucker Adult 603391 9409 Between Kohler Dams

White Sucker Young of year 603391 9410 Between Kohler Dams

White Sucker Young of year 603391 9411 Between Kohler Dams

White Sucker Young of year 603391 9412 Between Kohler Dams

PCB144/135 PCB146 PCB149 PCB151 PCB156

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

21 67 140 26

37 100 230 45

25 55 120 31

30 64 160 34

25 58 110 29 22

11 29 56 9.5 11

44 100 230 44

19 44 88 18

14 26 68 17

13 28 66 18

19 38 84 21 13

24 67 120 27 22

29 90 160 35

40 110 220 46

28 53 130 34

55 96 230 65

27 56 110 29 19
Table A3-4. Continued

Species Age Class Station Sample Location

Crayfish 603384 9409 Control

Crayfish 603384 9410 Control

Crayfish 603384 9411 Control

Crayfish 603385 9409 Rochester Park

Crayfish 603385 9410 Rochester Park

Crayfish 603385 9411 Rochester Park

Crayfish 603386 9409 Between Kohler Dams

Crayfish 603386 9410 Between Kohler Dams

Crayfish 603386 9411 Between Kohler Dams

Crayfish 603387 9409 Esselingen Park

Crayfish 603387 9410 Esselingen Park

Crayfish 603387 9411 Esselingen Park

Emergent Insects 603384 9401 Control

Emergent Insects 603384 9402 Control

Emergent Insects 603384 9403 Control

Emergent Insects 603384 9404 Control

Emergent Insects 603385 9401 Rochester Park

Emergent Insects 603385 9402 Rochester Park

Emergent Insects 603385 9403 Rochester Park

Emergent Insects 603385 9404 Rochester Park

Emergent Insects 603386 9401 Between Kohler Dams

Emergent Insects 603386 9402 Between Kohler Dams

Emergent Insects 603386 9403 Between Kohler Dams

Emergent Insects 603386 9404 Between Kohler Dams

Emergent Insects 603387 9401 Esselingen Park

Emergent Insects 603387 9402 Esselingen Park

Emergent Insects 603387 9403 Esselingen Park

Emergent Insects 603387 9404 Esselingen Park

Larval Insects 603384 9405 Control

Larval Insects 603384 9406 Control

Larval Insects 603384 9407 Control

Larval Insects 603384 9408 Control

Larval Insects 603385 9405 Rochester Park

Larval Insects 603385 9406 Rochester Park

Larval Insects 603385 9407 Rochester Park

Larval Insects 603385 9408 Rochester Park

Larval Insects 603386 9405 Between Kohler Dams

Larval Insects 603386 9406 Between Kohler Dams

Larval Insects 603386 9407 Between Kohler Dams

Larval Insects 603386 9408 Between Kohler Dams

Larval Insects 603387 9406 Esselingen Park

Larval Insects 603387 9407 Esselingen Park

Larval Insects 603387 9408 Esselingen Park

PCB157 PCB167 PCB169 PCB170/190 PCB171/202

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

1 U 1.8 U 1 U 2.5 U 0.8 U 

1 U 1.8 U 1 U 2.5 U 0.8 U 

1.8 U 2.5 U 0.8 U 

2.1 3.5 1 U 10

1.7 2.7 1 U 8.1

3.3 10 1.6 U*I

1.4 3.9 1 U 14 3 U*I

1.4 3.6 1 U 12 3 U*I

3.4 11 1.9 U*I

2.1 3.3 1 U 11 2.5 U*I

2.3 3.8 1 U 12 2.5 U*I

3.6 12 2.2 U*I

1 U 1.8 U 1 U 2.8 0.8 U

1 U 1.8 U 1 U 2.5 U 0.8 U

1 U 1.8 U 1 U 2.5 U 0.8 U

1 U 1.8 U 1 U 2.5 U 0.8 U

23 21 1 U 100 17 U*I

19 25 1 U 110 19 U*I

12 17 1 U 77 13 U*I

15 19 1 U 85 15 U*I

11 22 1 U 110 9.4

8.6 16 1 U 80 7.7 U*I

5.2 10 1 U 52 9 U*I

5.2 10 1 U 49 9 U*I

4.4 8.8 1 U 40 7 U*I

5.1 10 1 U 55 9 U*I

6.2 12 1 U 62 7 U*I

9.4 16 1 U 68 10 U*I

1 U 1.8 U 1 U 2.5 U 0.8 U

1.8 U 2.5 U 0.8 U

1 U 1.8 U 1 U 2.5 U 0.8 U

1 U 1.8 U 1 U 2.5 U 0.8 U

8.7 9.4 1 U 42 4.5 U*I

6.6 31 4.9 U*I

8.7 9.7 1 U 43 7.5 U*I

6.2 7.7 1 U 33 6 U*I

3.9 7.2 1 U 36 7 U*I

7.4 37 5.9 U*I

3 5.4 1 U 28 5 U*I

3.1 5 1 U 26 5 U*I

4 5.7 1 U 25 4.5 U*I

4.4 6.4 1 U 27 4.5 U*I

3.4 6.6 1 U 28 4.5 U*I
Table A3-4. Continued

Species Age Class Station Sample Location

Longnose Dace Young of year 603388 9413 Control

Longnose Dace Young of year 603388 9414 Control

Longnose Dace Young of year 603388 9415 Control

Longnose Dace Young of year 603389 9413 Rochester Park

Longnose Dace Young of year 603389 9414 Rochester Park

Longnose Dace Young of year 603389 9415 Rochester Park

Longnose Dace Young of year 603390 9413 Esselingen Park

Longnose Dace Young of year 603390 9414 Esselingen Park

Longnose Dace Young of year 603390 9415 Esselingen Park

Longnose Dace Young of year 603391 9413 Between Kohler Dams

Longnose Dace Young of year 603391 9414 Between Kohler Dams

Longnose Dace Young of year 603391 9415 Between Kohler Dams

Smallmouth Bass Adult 603388 9401 Control

Smallmouth Bass Adult 603388 9402 Control

Smallmouth Bass Adult 603388 9403 Control

Smallmouth Bass Young of year 603388 9404 Control

Smallmouth Bass Young of year 603388 9405 Control

Smallmouth Bass Young of year 603388 9406 Control

Smallmouth Bass Adult 603389 9401 Rochester Park

Smallmouth Bass Adult 603389 9402 Rochester Park

Smallmouth Bass Adult 603389 9403 Rochester Park

Smallmouth Bass Young of year 603389 9404 Rochester Park

Smallmouth Bass Young of year 603389 9405 Rochester Park

Smallmouth Bass Young of year 603389 9406 Rochester Park

Smallmouth Bass Adult 603390 9401 Esselingen Park

Smallmouth Bass Adult 603390 9402 Esselingen Park

Smallmouth Bass Adult 603390 9403 Esselingen Park

Smallmouth Bass Young of year 603390 9404 Esselingen Park

Smallmouth Bass Young of year 603390 9405 Esselingen Park

Smallmouth Bass Young of year 603390 9406 Esselingen Park

Smallmouth Bass Adult 603391 9401 Between Kohler Dams

Smallmouth Bass Adult 603391 9402 Between Kohler Dams

Smallmouth Bass Adult 603391 9403 Between Kohler Dams

Smallmouth Bass Young of year 603391 9404 Between Kohler Dams

Smallmouth Bass Young of year 603391 9405 Between Kohler Dams

Smallmouth Bass Young of year 603391 9406 Between Kohler Dams

White Sucker Adult 603388 9407 Control

White Sucker Adult 603388 9408 Control

White Sucker Adult 603388 9409 Control

White Sucker Young of year 603388 9410 Control

White Sucker Young of year 603388 9411 Control

White Sucker Young of year 603388 9412 Control

White Sucker Adult 603389 9407 Rochester Park

PCB157 PCB167 PCB169 PCB170/190 PCB171/202

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

1 U 1.8 U 1 U 4.5 0.8 U

1.8 U 2.5 U 0.8 U

1.8 U 2.5 U 0.8 U

11 53 10 U*I

12 56 10 U*I

11 19 1 U 82 13 U*I

26 28 1 U 110 13 U*I

22 110 9.8 U*I

14 71 6.6 U*I

16 80 12 U*I

19 93 14 U*I

19 22 1 U 94 10 U*I

1 U 1.8 U 1 U 6.5 0.81

1 U 1.8 U 1 U 3.3 0.8 U 

1 U 1.8 U 1 U 2.5 U 0.8 U 

1.8 U 2.5 U 0.8 U 

1.8 U 2.5 U 0.8 U 

1 U 1.8 U 1 U 2.5 U 0.8 U 

52 70 3 U*D 310 60 U*I

54 71 1.5 U*D 290 55 U*I

27 39 1.7 U*D 140 30 U*I

32 110 10 U*I

38 130 20 U*I

16 24 1 U 110 17 U*I

10 20 1 U 84 14 U*I

11 25 1 U 110 14 U*I

11 27 1 U 110 14 U*I

9.3 45 10 U*I

13 64 11 U*I

6.8 13 1 U 58 9.2 U*I

49 56 1 U 170 19 U*I

30 34 1 U 130 15 U*I

36 66 1 U 220 24 U*I

27 140 13 U*I

24 110 11 U*I

36 58 1 U 220 25 U*I

1 U 1.8 U 1 U 2.5 U 0.8 U

1.8 U 2.5 U 0.8 U

1.8 U 2.5 U 0.8 U

1.8 U 5.5 U*I 0.8 U

1.8 U 2.5 U 0.8 U

1 U 1.8 U 1 U 2.5 U 0.8 U

6.4 13 1 U 63 11 U*I
Table A3-4. Continued

Species Age Class Station Sample Location

White Sucker Adult 603389 9408 Rochester Park

White Sucker Adult 603389 9409 Rochester Park

White Sucker Young of year 603389 9410 Rochester Park

White Sucker Young of year 603389 9411 Rochester Park

White Sucker Young of year 603389 9412 Rochester Park

White Sucker Adult 603390 9407 Esselingen Park

White Sucker Adult 603390 9408 Esselingen Park

White Sucker Adult 603390 9409 Esselingen Park

White Sucker Young of year 603390 9410 Esselingen Park

White Sucker Young of year 603390 9411 Esselingen Park

White Sucker Young of year 603390 9412 Esselingen Park

White Sucker Adult 603391 9407 Between Kohler Dams

White Sucker Adult 603391 9408 Between Kohler Dams

White Sucker Adult 603391 9409 Between Kohler Dams

White Sucker Young of year 603391 9410 Between Kohler Dams

White Sucker Young of year 603391 9411 Between Kohler Dams

White Sucker Young of year 603391 9412 Between Kohler Dams

PCB157 PCB167 PCB169 PCB170/190 PCB171/202

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

16 81 15 U*I

24 120 20 U*I

13 69 12 U*I

13 70 11 U*I

6 10 1 U 52 9.3 U*I

2.6 4.7 1 U 24

20 110 20 U*I

8.1 45 10 U*I

5.4 42 2.8 U*I

4.3 41 3.3 U*I

5.5 6.2 1 U 29 4 U*I

10 12 1 U 55 7.5 U*I

15 73 7.8 U*I

17 86 8.5 U*I

7.5 50 5.4 U*I

12 72 8.4 U*I

7.2 8.5 1 U 42 6 U*I
Table A3-4. Continued

Species Age Class Station Sample Location

Crayfish 603384 9409 Control

Crayfish 603384 9410 Control

Crayfish 603384 9411 Control

Crayfish 603385 9409 Rochester Park

Crayfish 603385 9410 Rochester Park

Crayfish 603385 9411 Rochester Park

Crayfish 603386 9409 Between Kohler Dams

Crayfish 603386 9410 Between Kohler Dams

Crayfish 603386 9411 Between Kohler Dams

Crayfish 603387 9409 Esselingen Park

Crayfish 603387 9410 Esselingen Park

Crayfish 603387 9411 Esselingen Park

Emergent Insects 603384 9401 Control

Emergent Insects 603384 9402 Control

Emergent Insects 603384 9403 Control

Emergent Insects 603384 9404 Control

Emergent Insects 603385 9401 Rochester Park

Emergent Insects 603385 9402 Rochester Park

Emergent Insects 603385 9403 Rochester Park

Emergent Insects 603385 9404 Rochester Park

Emergent Insects 603386 9401 Between Kohler Dams

Emergent Insects 603386 9402 Between Kohler Dams

Emergent Insects 603386 9403 Between Kohler Dams

Emergent Insects 603386 9404 Between Kohler Dams

Emergent Insects 603387 9401 Esselingen Park

Emergent Insects 603387 9402 Esselingen Park

Emergent Insects 603387 9403 Esselingen Park

Emergent Insects 603387 9404 Esselingen Park

Larval Insects 603384 9405 Control

Larval Insects 603384 9406 Control

Larval Insects 603384 9407 Control

Larval Insects 603384 9408 Control

Larval Insects 603385 9405 Rochester Park

Larval Insects 603385 9406 Rochester Park

Larval Insects 603385 9407 Rochester Park

Larval Insects 603385 9408 Rochester Park

Larval Insects 603386 9405 Between Kohler Dams

Larval Insects 603386 9406 Between Kohler Dams

Larval Insects 603386 9407 Between Kohler Dams

Larval Insects 603386 9408 Between Kohler Dams

Larval Insects 603387 9406 Esselingen Park

Larval Insects 603387 9407 Esselingen Park

Larval Insects 603387 9408 Esselingen Park

PCB172/197 PCB174 PCB177 PCB178 PCB180

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

1.8 U 1.1 U 1.1 U 1.3 U 2.2 U 

1.8 U 1.1 U 1.1 U 1.3 U 2.2 U 

1.8 U 1.1 U 1.1 U 1.3 U 2.2 U 

4.2 4.8 2 17

2.9 3.7 13

2.1 3.4 4.5 2 14

2.9 5.1 7.1 3.6 20

2.6 4.7 6.5 3.4 18

2.4 4.1 5.7 2.9 16

2.3 4.2 5.7 3.5 16

2.6 4.3 5.8 3.3 18

2.7 4.4 6.1 3.1 18

1.8 U 1.1 U 1.1 U 1.3 U 4

1.8 U 1.1 U 1.5 U*I 1.3 U 2.4

1.8 U 1.1 U 1.1 U 1.3 U 2.3

1.8 U 1.1 U 1.1 U 1.3 U 2.3

14 27 28 12 95

15 30 30 12 110

11 21 23 8.9 76

12 24 26 10 80

16 30 38 18 100

12 21 28 13 76

7.9 15 19 8.7 52

7.4 13 17 7.8 49

5.6 9.5 13 5.3 40

8.2 14 19 8.7 54

9.6 18 23 11 63

11 19 24 11 67

1.8 U 1.1 U 1.1 U 1.3 U 2.2 U 

1.8 U 1.1 U 1.1 U 1.3 U 2.2 U 

1.8 U 1.1 U 1.1 U 1.3 U 2.2 U 

1.8 U 1.1 U 1.1 U 1.3 U 2.2 U 

5.4 12 11 4.1 41

4 8.9 8.2 3.1 28

5.6 13 12 4.2 41

4.4 10 8.6 3.3 31

5.1 10 13 5.4 36

5.3 9.7 13 5.8 35

4.1 8.3 10 4.6 27

3.8 8.3 9.6 4.7 25

3.7 7.4 8.8 4 25

3.9 7.9 8.7 4.3 25

4.1 8.2 9.5 4.6 26
Table A3-4. Continued

Species Age Class Station Sample Location

Longnose Dace Young of year 603388 9413 Control

Longnose Dace Young of year 603388 9414 Control

Longnose Dace Young of year 603388 9415 Control

Longnose Dace Young of year 603389 9413 Rochester Park

Longnose Dace Young of year 603389 9414 Rochester Park

Longnose Dace Young of year 603389 9415 Rochester Park

Longnose Dace Young of year 603390 9413 Esselingen Park

Longnose Dace Young of year 603390 9414 Esselingen Park

Longnose Dace Young of year 603390 9415 Esselingen Park

Longnose Dace Young of year 603391 9413 Between Kohler Dams

Longnose Dace Young of year 603391 9414 Between Kohler Dams

Longnose Dace Young of year 603391 9415 Between Kohler Dams

Smallmouth Bass Adult 603388 9401 Control

Smallmouth Bass Adult 603388 9402 Control

Smallmouth Bass Adult 603388 9403 Control

Smallmouth Bass Young of year 603388 9404 Control

Smallmouth Bass Young of year 603388 9405 Control

Smallmouth Bass Young of year 603388 9406 Control

Smallmouth Bass Adult 603389 9401 Rochester Park

Smallmouth Bass Adult 603389 9402 Rochester Park

Smallmouth Bass Adult 603389 9403 Rochester Park

Smallmouth Bass Young of year 603389 9404 Rochester Park

Smallmouth Bass Young of year 603389 9405 Rochester Park

Smallmouth Bass Young of year 603389 9406 Rochester Park

Smallmouth Bass Adult 603390 9401 Esselingen Park

Smallmouth Bass Adult 603390 9402 Esselingen Park

Smallmouth Bass Adult 603390 9403 Esselingen Park

Smallmouth Bass Young of year 603390 9404 Esselingen Park

Smallmouth Bass Young of year 603390 9405 Esselingen Park

Smallmouth Bass Young of year 603390 9406 Esselingen Park

Smallmouth Bass Adult 603391 9401 Between Kohler Dams

Smallmouth Bass Adult 603391 9402 Between Kohler Dams

Smallmouth Bass Adult 603391 9403 Between Kohler Dams

Smallmouth Bass Young of year 603391 9404 Between Kohler Dams

Smallmouth Bass Young of year 603391 9405 Between Kohler Dams

Smallmouth Bass Young of year 603391 9406 Between Kohler Dams

White Sucker Adult 603388 9407 Control

White Sucker Adult 603388 9408 Control

White Sucker Adult 603388 9409 Control

White Sucker Young of year 603388 9410 Control

White Sucker Young of year 603388 9411 Control

White Sucker Young of year 603388 9412 Control

White Sucker Adult 603389 9407 Rochester Park

PCB172/197 PCB174 PCB177 PCB178 PCB180

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

1.8 U 1.5 1.4 1.3 U 5.8

1.8 U 1.1 U 1.1 U 1.3 U 2.9

1.8 U 1.1 U 1.1 U 1.3 U 2.2 U 

6.9 13 12 17 53

7.5 14 13 4.5 57

11 22 20 7.1 77

18 31 36 16 120

16 28 33 14 100

12 20 23 10 76

12 20 25 11 82

15 24 31 14 95

15 25 33 15 99

1.8 U 2 1.7 1.3 U 7.8

1.8 U 1.2 1.1 U 1.3 U 4.8

1.8 U 1.1 U 1.1 U 1.3 U 3.2

1.8 U 1.1 U 1.1 U 1.3 U 2.2 U 

1.8 U 1.1 U 1.1 U 1.3 U 2.2 U 

1.8 U 1.1 U 1.1 U 2.4

47 75 86 35 300

48 77 85 36 290

24 43 46 19 160

14 27 29 11 100

18 32 34 13 120

15 27 27 11 100

13 22 27 12 86

18 29 36 18 110

20 30 39 19 120

6.2 12 14 5.9 48

9.3 17 21 8.3 68

8.8 15 19 8.4 60

33 51 66 33 210

22 33 43 21 140

36 56 66 31 220

18 33 39 17 110

15 27 33 15 100

32 56 61 28 200

1.8 U 1.1 U 1.1 U 1.3 U 2.5

1.8 U 1.1 U 1.1 U 1.3 U 2.2 U 

1.8 U 1.1 U 1.1 U 1.3 U 2.2 U 

1.8 U 1.1 U 1.1 U 1.3 U 2.2 U 

1.8 U 1.1 U 1.1 U 1.3 U 2.2 U 

1.8 U 1.1 U 1.1 U 1.3 U 2.2 U 

8.6 8.5 15 5.8 57
Table A3-4. Continued

Species Age Class Station Sample Location

White Sucker Adult 603389 9408 Rochester Park

White Sucker Adult 603389 9409 Rochester Park

White Sucker Young of year 603389 9410 Rochester Park

White Sucker Young of year 603389 9411 Rochester Park

White Sucker Young of year 603389 9412 Rochester Park

White Sucker Adult 603390 9407 Esselingen Park

White Sucker Adult 603390 9408 Esselingen Park

White Sucker Adult 603390 9409 Esselingen Park

White Sucker Young of year 603390 9410 Esselingen Park

White Sucker Young of year 603390 9411 Esselingen Park

White Sucker Young of year 603390 9412 Esselingen Park

White Sucker Adult 603391 9407 Between Kohler Dams

White Sucker Adult 603391 9408 Between Kohler Dams

White Sucker Adult 603391 9409 Between Kohler Dams

White Sucker Young of year 603391 9410 Between Kohler Dams

White Sucker Young of year 603391 9411 Between Kohler Dams

White Sucker Young of year 603391 9412 Between Kohler Dams

PCB172/197 PCB174 PCB177 PCB178 PCB180

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

11 13 20 6.8 75

15 18 29 10 110

8.7 14 17 6.5 65

9 14 18 6.9 63

7.2 11 14 6.3 48

3.7 4.3 7.2 3.3 23

16 21 31 13 100

6.4 9 13 5.7 43

4.1 5.6 8.3 4.4 21

3.9 5.3 8 3.8 22

4.3 7.1 10 4.8 29

8.1 8.8 17 7.7 54

9.6 10 21 8.8 65

13 17 27 11 80

5.7 9.7 14 6.4 39

9.2 16 24 11 62

6.2 9.1 15 7.1 41
Table A3-4. Continued

Species Age Class Station Sample Location

Crayfish 603384 9409 Control

Crayfish 603384 9410 Control

Crayfish 603384 9411 Control

Crayfish 603385 9409 Rochester Park

Crayfish 603385 9410 Rochester Park

Crayfish 603385 9411 Rochester Park

Crayfish 603386 9409 Between Kohler Dams

Crayfish 603386 9410 Between Kohler Dams

Crayfish 603386 9411 Between Kohler Dams

Crayfish 603387 9409 Esselingen Park

Crayfish 603387 9410 Esselingen Park

Crayfish 603387 9411 Esselingen Park

Emergent Insects 603384 9401 Control

Emergent Insects 603384 9402 Control

Emergent Insects 603384 9403 Control

Emergent Insects 603384 9404 Control

Emergent Insects 603385 9401 Rochester Park

Emergent Insects 603385 9402 Rochester Park

Emergent Insects 603385 9403 Rochester Park

Emergent Insects 603385 9404 Rochester Park

Emergent Insects 603386 9401 Between Kohler Dams

Emergent Insects 603386 9402 Between Kohler Dams

Emergent Insects 603386 9403 Between Kohler Dams

Emergent Insects 603386 9404 Between Kohler Dams

Emergent Insects 603387 9401 Esselingen Park

Emergent Insects 603387 9402 Esselingen Park

Emergent Insects 603387 9403 Esselingen Park

Emergent Insects 603387 9404 Esselingen Park

Larval Insects 603384 9405 Control

Larval Insects 603384 9406 Control

Larval Insects 603384 9407 Control

Larval Insects 603384 9408 Control

Larval Insects 603385 9405 Rochester Park

Larval Insects 603385 9406 Rochester Park

Larval Insects 603385 9407 Rochester Park

Larval Insects 603385 9408 Rochester Park

Larval Insects 603386 9405 Between Kohler Dams

Larval Insects 603386 9406 Between Kohler Dams

Larval Insects 603386 9407 Between Kohler Dams

Larval Insects 603386 9408 Between Kohler Dams

Larval Insects 603387 9406 Esselingen Park

Larval Insects 603387 9407 Esselingen Park

Larval Insects 603387 9408 Esselingen Park

PCB183 PCB185 PCB187/182 PCB194 PCB195/208

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

1.8 U 1 U 1.5 U 1 U 2 U 

1.8 U 1 U 1.5 U 1 U 2 U 

1.8 U 1 U 1.5 U 1 U 2 U 

3.2 8.8 2.4

2.3 6.9 1.4

2.8 7.9 1.6

4 13 2.4 2.3

3.7 12 2 2 U 

3.1 11 1.8

3.2 1 U 11 2.3 2.4

3.4 1 U 11 2.5 2.3

3.4 1 U 11 1.9 2 U 

1.8 U 1 U 1.8 1 U 2 U 

1.8 U 1 U 1.5 U 1 U 2 U 

1.8 U 1 U 1.5 U 1 U 2 U 

1.8 U 1 U 1.5 U 1 U 2 U 

28 2.5 45 16 14

33 3 50 15 14

21 2.1 35 12 11

24 2.2 39 12 11

31 2.6 61 18 16

23 2 44 13 11

15 31 8.8 7.7

15 28 7.9 6.8

13 23 4.7 4.5

16 31 8 7.1

19 1.7 37 8.8 8.2

20 39 11 9.5

1.8 U 1 U 1.5 U 1 U 2 U 

1.8 U 1 U 1.5 U 1 U 2 U 

1.8 U 1 U 1.5 U 1 U 2 U 

1.8 U 1 U 1.5 U 1 U 2 U 

12 18 5.9 5

8.4 1 U 12 3.8 3.5

12 18 5.2 4.6

9.1 13 4.1 3.9

11 21 4.8 4.2

10 20 5.4 4.3

8.7 17 3.7 3.4

7.8 16 3.3 3.2

7.3 14 3.7 3.1

7.4 1 U 14 3.9 3.4

7.7 1 U 15 3.9 3.4
Table A3-4. Continued

Species Age Class Station Sample Location

Longnose Dace Young of year 603388 9413 Control

Longnose Dace Young of year 603388 9414 Control

Longnose Dace Young of year 603388 9415 Control

Longnose Dace Young of year 603389 9413 Rochester Park

Longnose Dace Young of year 603389 9414 Rochester Park

Longnose Dace Young of year 603389 9415 Rochester Park

Longnose Dace Young of year 603390 9413 Esselingen Park

Longnose Dace Young of year 603390 9414 Esselingen Park

Longnose Dace Young of year 603390 9415 Esselingen Park

Longnose Dace Young of year 603391 9413 Between Kohler Dams

Longnose Dace Young of year 603391 9414 Between Kohler Dams

Longnose Dace Young of year 603391 9415 Between Kohler Dams

Smallmouth Bass Adult 603388 9401 Control

Smallmouth Bass Adult 603388 9402 Control

Smallmouth Bass Adult 603388 9403 Control

Smallmouth Bass Young of year 603388 9404 Control

Smallmouth Bass Young of year 603388 9405 Control

Smallmouth Bass Young of year 603388 9406 Control

Smallmouth Bass Adult 603389 9401 Rochester Park

Smallmouth Bass Adult 603389 9402 Rochester Park

Smallmouth Bass Adult 603389 9403 Rochester Park

Smallmouth Bass Young of year 603389 9404 Rochester Park

Smallmouth Bass Young of year 603389 9405 Rochester Park

Smallmouth Bass Young of year 603389 9406 Rochester Park

Smallmouth Bass Adult 603390 9401 Esselingen Park

Smallmouth Bass Adult 603390 9402 Esselingen Park

Smallmouth Bass Adult 603390 9403 Esselingen Park

Smallmouth Bass Young of year 603390 9404 Esselingen Park

Smallmouth Bass Young of year 603390 9405 Esselingen Park

Smallmouth Bass Young of year 603390 9406 Esselingen Park

Smallmouth Bass Adult 603391 9401 Between Kohler Dams

Smallmouth Bass Adult 603391 9402 Between Kohler Dams

Smallmouth Bass Adult 603391 9403 Between Kohler Dams

Smallmouth Bass Young of year 603391 9404 Between Kohler Dams

Smallmouth Bass Young of year 603391 9405 Between Kohler Dams

Smallmouth Bass Young of year 603391 9406 Between Kohler Dams

White Sucker Adult 603388 9407 Control

White Sucker Adult 603388 9408 Control

White Sucker Adult 603388 9409 Control

White Sucker Young of year 603388 9410 Control

White Sucker Young of year 603388 9411 Control

White Sucker Young of year 603388 9412 Control

White Sucker Adult 603389 9407 Rochester Park

PCB183 PCB185 PCB187/182 PCB194 PCB195/208

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

1.8 U 1 U 2.7 1 U 2 U 

1.8 U 1 U 1.5 U 1 U 2 U 

1.8 U 1 U 1.5 U 1 U 2 U 

14 21 6.9 5.6

15 23 7

23 3 32 8.9 7.9

37 4.3 65 14 12

31 3.9 60 13 11

22 3.1 44 8.5 8.3

24 2.7 44 11 9.4

29 3.3 53 12 11

29 3.3 56 13 11

2.2 1 U 3.5 1.2 2 U 

1.8 U 1 U 2.3 1 U 2 U 

1.8 U 1.6 1 U 2 U 

1.8 U 1 U 1.5 U 1 U 2 U 

1.8 U 1 U 1.5 U 1 U 2 U 

1.8 U 1 U 1.5 U 1 U 2 U 

90 12 140 45 43

90 12 140 48 43

44 5.7 77 22 20

28 3.9 45 12 11

37 4.9 69 16 15

30 4 45 14 13

25 3.2 50 9.7 9.6

33 4.3 71 14 15

35 4.2 74 16 16

13 25 5.8 4.9

19 37 8.3 7.4

18 2.5 35 6.5 6.2

53 6.5 120 26 24

39 4.7 81 19 18

65 8.3 120 30 26

34 4.5 65 14 13

31 2.8 54 15 9.9

67 8 110 23 23

1.8 U 1 U 1.5 U 1 U 2 U 

1.8 U 1 U 1.5 U 1 U 2 U 

1.8 U 1 U 1.5 U 1 U 2 U 

1.8 U 1 U 1.5 U 1 U 2 U 

1.8 U 1 U 1.5 U 1 U 2 U 

1.8 U 1 U 1.5 U 1 U 

17 26 8.4 8.8
Table A3-4. Continued

Species Age Class Station Sample Location

White Sucker Adult 603389 9408 Rochester Park

White Sucker Adult 603389 9409 Rochester Park

White Sucker Young of year 603389 9410 Rochester Park

White Sucker Young of year 603389 9411 Rochester Park

White Sucker Young of year 603389 9412 Rochester Park

White Sucker Adult 603390 9407 Esselingen Park

White Sucker Adult 603390 9408 Esselingen Park

White Sucker Adult 603390 9409 Esselingen Park

White Sucker Young of year 603390 9410 Esselingen Park

White Sucker Young of year 603390 9411 Esselingen Park

White Sucker Young of year 603390 9412 Esselingen Park

White Sucker Adult 603391 9407 Between Kohler Dams

White Sucker Adult 603391 9408 Between Kohler Dams

White Sucker Adult 603391 9409 Between Kohler Dams

White Sucker Young of year 603391 9410 Between Kohler Dams

White Sucker Young of year 603391 9411 Between Kohler Dams

White Sucker Young of year 603391 9412 Between Kohler Dams

PCB183 PCB185 PCB187/182 PCB194 PCB195/208

(μg/kg) (μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q Conc Q

22 30 11 11

30 3.6 48 16 15

19 2.5 27 10 11

19 30 8.8 9.2

15 25 6.8 7.4

7 13 3.3

29 3.5 55 15 15

12 23 6.5 6.4

7.3 1.2 14 3.2 3.8

7.2 1.1 15 3 3.8

9 18 3.7

17 31 7.7 7.8

21 2.2 40 9.2 9.3

24 2.8 53 11 11

13 1.6 25 4.7 5.4

21 2.5 42 8.3 9.1

13 25 5.4 5.8
Table A3-4. Continued

Species Age Class Station Sample Location

Crayfish 603384 9409 Control

Crayfish 603384 9410 Control

Crayfish 603384 9411 Control

Crayfish 603385 9409 Rochester Park

Crayfish 603385 9410 Rochester Park

Crayfish 603385 9411 Rochester Park

Crayfish 603386 9409 Between Kohler Dams

Crayfish 603386 9410 Between Kohler Dams

Crayfish 603386 9411 Between Kohler Dams

Crayfish 603387 9409 Esselingen Park

Crayfish 603387 9410 Esselingen Park

Crayfish 603387 9411 Esselingen Park

Emergent Insects 603384 9401 Control

Emergent Insects 603384 9402 Control

Emergent Insects 603384 9403 Control

Emergent Insects 603384 9404 Control

Emergent Insects 603385 9401 Rochester Park

Emergent Insects 603385 9402 Rochester Park

Emergent Insects 603385 9403 Rochester Park

Emergent Insects 603385 9404 Rochester Park

Emergent Insects 603386 9401 Between Kohler Dams

Emergent Insects 603386 9402 Between Kohler Dams

Emergent Insects 603386 9403 Between Kohler Dams

Emergent Insects 603386 9404 Between Kohler Dams

Emergent Insects 603387 9401 Esselingen Park

Emergent Insects 603387 9402 Esselingen Park

Emergent Insects 603387 9403 Esselingen Park

Emergent Insects 603387 9404 Esselingen Park

Larval Insects 603384 9405 Control

Larval Insects 603384 9406 Control

Larval Insects 603384 9407 Control

Larval Insects 603384 9408 Control

Larval Insects 603385 9405 Rochester Park

Larval Insects 603385 9406 Rochester Park

Larval Insects 603385 9407 Rochester Park

Larval Insects 603385 9408 Rochester Park

Larval Insects 603386 9405 Between Kohler Dams

Larval Insects 603386 9406 Between Kohler Dams

Larval Insects 603386 9407 Between Kohler Dams

Larval Insects 603386 9408 Between Kohler Dams

Larval Insects 603387 9406 Esselingen Park

Larval Insects 603387 9407 Esselingen Park

Larval Insects 603387 9408 Esselingen Park

PCB196/203 PCB199 PCB201 PCB206

(μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q

3 U 0.6 U 1.8 U 1.5 U 

3 U 0.6 U 1.8 U 1.5 U 

3 U 0.6 U 1.8 U 1.5 U 

3.8

2.4

2.6

3 U 4.6 1.5 U 

3 U 3.7 1.5 U 

0.6 U 3.3 1.5 U 

3 U 0.6 U 4 1.5 U 

3 U 0.6 U 4 1.5 U 

3 U 3.6 1.5 U 

3 U 0.6 U 1.8 U 1.5 U 

3 U 0.6 U 1.8 U 1.5 U 

3 U 0.6 U 1.8 U 1.5 U 

3 U 0.6 U 1.8 U 1.5 U 

23 19 6.1

26 19 5.6

18 15 4.4

19 15 4.3

28 22 6.5

21 16 5.1

15 12 3.5

13 10 3

9.2 6.3

15 11 3.1

18 13 3.4

17 14 3.6

3 U 0.6 U 1.8 U 1.5 U 

3 U 0.6 U 1.8 U 1.5 U 

3 U 0.6 U 1.8 U 1.5 U 

3 U 0.6 U 1.8 U 1.5 U 

9.5 7.1

6.2 0.6 U 5.1

8.6 6.5 1.6

6.4 5.3

8.9 6.2 1.5

8.2 6.7

6.9 5.2

6.1 5.1

5.9 4.7

5.9 0.6 U 5 1.5 U 

6.1 0.6 U 5.2 1.5 U 
Table A3-4. Continued

Species Age Class Station Sample Location

Longnose Dace Young of year 603388 9413 Control

Longnose Dace Young of year 603388 9414 Control

Longnose Dace Young of year 603388 9415 Control

Longnose Dace Young of year 603389 9413 Rochester Park

Longnose Dace Young of year 603389 9414 Rochester Park

Longnose Dace Young of year 603389 9415 Rochester Park

Longnose Dace Young of year 603390 9413 Esselingen Park

Longnose Dace Young of year 603390 9414 Esselingen Park

Longnose Dace Young of year 603390 9415 Esselingen Park

Longnose Dace Young of year 603391 9413 Between Kohler Dams

Longnose Dace Young of year 603391 9414 Between Kohler Dams

Longnose Dace Young of year 603391 9415 Between Kohler Dams

Smallmouth Bass Adult 603388 9401 Control

Smallmouth Bass Adult 603388 9402 Control

Smallmouth Bass Adult 603388 9403 Control

Smallmouth Bass Young of year 603388 9404 Control

Smallmouth Bass Young of year 603388 9405 Control

Smallmouth Bass Young of year 603388 9406 Control

Smallmouth Bass Adult 603389 9401 Rochester Park

Smallmouth Bass Adult 603389 9402 Rochester Park

Smallmouth Bass Adult 603389 9403 Rochester Park

Smallmouth Bass Young of year 603389 9404 Rochester Park

Smallmouth Bass Young of year 603389 9405 Rochester Park

Smallmouth Bass Young of year 603389 9406 Rochester Park

Smallmouth Bass Adult 603390 9401 Esselingen Park

Smallmouth Bass Adult 603390 9402 Esselingen Park

Smallmouth Bass Adult 603390 9403 Esselingen Park

Smallmouth Bass Young of year 603390 9404 Esselingen Park

Smallmouth Bass Young of year 603390 9405 Esselingen Park

Smallmouth Bass Young of year 603390 9406 Esselingen Park

Smallmouth Bass Adult 603391 9401 Between Kohler Dams

Smallmouth Bass Adult 603391 9402 Between Kohler Dams

Smallmouth Bass Adult 603391 9403 Between Kohler Dams

Smallmouth Bass Young of year 603391 9404 Between Kohler Dams

Smallmouth Bass Young of year 603391 9405 Between Kohler Dams

Smallmouth Bass Young of year 603391 9406 Between Kohler Dams

White Sucker Adult 603388 9407 Control

White Sucker Adult 603388 9408 Control

White Sucker Adult 603388 9409 Control

White Sucker Young of year 603388 9410 Control

White Sucker Young of year 603388 9411 Control

White Sucker Young of year 603388 9412 Control

White Sucker Adult 603389 9407 Rochester Park

PCB196/203 PCB199 PCB201 PCB206

(μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q

3 U 0.6 U 1.8 U 1.5 U 

3 U 0.6 U 1.8 U 1.5 U 

3 U 0.6 U 1.8 U 1.5 U 

11 7.2

11 7.6

15 10 3.3

25 19 4

22 17 3.7

17 12 2.6

18 13

22 0.6 U 16 4.3

23 17 3.9

3 U 0.6 U 1.9 1.6 U*I

3 U 1.8 U 1.5 U 

1.8 U 1.5 U 

3 U 0.6 U 1.8 U 1.5 U 

3 U 0.6 U 1.8 U 1.5 U 

3 U 1.8 U 1.5 U 

70 1.9 54 16

72 1.9 57 16

33 0.95 28 6.8

20 16 3.9

26 0.6 U 20 5

22 17

17 13 3.2

25 22 5.8

26 24 5.4

10 7.1

15 9.9

13 8.7 2

37 39 7.2

30 27 6

47 40 9.3

24 0.63 20 4.5

23 19 3.7

40 33 8

3 U 1.8 U 1.5 U 

3 U 0.6 U 1.8 U 1.5 U 

3 U 0.6 U 1.8 U 1.5 U 

3 U 0.6 U 1.8 U 1.5 U 

3 U 0.6 U 1.8 U 1.5 U 

3 U 1.8 U 1.5 U 

14 9.7 3.9
Table A3-4. Continued

Species Age Class Station Sample Location

White Sucker Adult 603389 9408 Rochester Park

White Sucker Adult 603389 9409 Rochester Park

White Sucker Young of year 603389 9410 Rochester Park

White Sucker Young of year 603389 9411 Rochester Park

White Sucker Young of year 603389 9412 Rochester Park

White Sucker Adult 603390 9407 Esselingen Park

White Sucker Adult 603390 9408 Esselingen Park

White Sucker Adult 603390 9409 Esselingen Park

White Sucker Young of year 603390 9410 Esselingen Park

White Sucker Young of year 603390 9411 Esselingen Park

White Sucker Young of year 603390 9412 Esselingen Park

White Sucker Adult 603391 9407 Between Kohler Dams

White Sucker Adult 603391 9408 Between Kohler Dams

White Sucker Adult 603391 9409 Between Kohler Dams

White Sucker Young of year 603391 9410 Between Kohler Dams

White Sucker Young of year 603391 9411 Between Kohler Dams

White Sucker Young of year 603391 9412 Between Kohler Dams

PCB196/203 PCB199 PCB201 PCB206

(μg/kg) (μg/kg) (μg/kg) (μg/kg)

Conc Q Conc Q Conc Q Conc Q

17 13 4.2

25 19 5.7

17 13 4.5

14 12 3.5

12 8.8 2.8

5.7 4.2

25 20 5.8

11 8.6

6.4 6

6.2 5.1

7.1 5.8

14 10

16 12 3.4

20 15 3.9

9.2 7.2 1.8

15 12 3

7.8
Table A3-3. Results of the metals analyses of sediment samples collected for the WDNR food chain study

Station ID 603363 603364 603365 603365 603366 603367

Sample ID 6A 6B 6C 6DUP 6D 6E 

Chemical Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

Arsenic 1.35 1.15 1.86 1.86 1.75 0.65

Cadmium 0.4 U 0.4 U 0.4 U 0.4 U 1.3 0.4 U 

Chromium 22 18 28 26 25 19

Copper 37 25 39 45 36 24

Lead 38 63 58 55 59 48

Mercury 0.068 0.054 0.11 0.13 0.14 0.033

Selenium 0.32 0.19 0.44 0.31 0.62 0.2

Silver 2.5 U 2.5 U 2.5 U 2.5 U 2.5 U 2.5 U 

NOTE: All concentrations are in mg/kg
Table A3-3. Results of the metals analyses of sediment samples collected for the WDNR food chain study

Station ID

Sample ID

Chemical

Arsenic

Cadmium

Chromium

Copper

Lead

Mercury

Selenium

Silver

603368 603368 603369 603370 603371 603372

5A 5DUP 5B 5C 5D 3A 

Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

1.77 1.51 0.84 0.61 0.77 1.03

0.4 U 1.1 2.2 0.7 0.4 U 0.4 U 

26 24 16 14 16 14

39 39 23 20 28 15

59 56 37 30 34 12

0.08 0.083 0.036 0.024 0.031 0.064

0.64 0.4 0.25 0.12 0.23 0.17

2.5 U 2.5 U 2.5 U 2.5 U 2.5 U 2.5 U 

NOTE: All concentrations are in mg/kg
Table A3-3. Results of the metals analyses of sediment samples collected for the WDNR food chain study

Station ID

Sample ID

Chemical

Arsenic

Cadmium

Chromium

Copper

Lead

Mercury

Selenium

Silver

603373 603374 603375 603375 603376 603377

3B 3C 3D 3DUP 3E 2A 

Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

0.81 0.53 0.96 1.15 0.94 0.77

0.9 0.7 0.4 U 1.2 0.4 U 0.4 U 

12 8.4 17 14 14 11

11 7.8 23 18 14 13

10 11 16 17 12 11

0.051 0.022 0.076 0.075 0.06 0.051

0.37 0.08 0.33 0.32 0.3 0.16

2.5 U 2.5 U 2.5 U 2.5 U 2.5 U 2.5 U 

NOTE: All concentrations are in mg/kg
Table A3-3. Results of the metals analyses of sediment samples collected for the WDNR food chain study

Station ID

Sample ID

Chemical

Arsenic

Cadmium

Chromium

Copper

Lead

Mercury

Selenium

Silver

603378 603379 603380 603381 603392 603393

2B 2C 2D 2E 1A 1B 

Conc Q Conc Q Conc Q Conc Q Conc Q Conc Q

0.98 0.91 1.02 0.72 1.16 1.17

2.2 1 0.4 U 0.4 U 0.4 U 0.4 U 

14 12 22 7.3 10 16

44 16 87 7.2 10 14

13 12 53 8 5 11

0.05 0.056 0.27 0.044 0.036 0.06

0.36 0.22 0.41 0.15 0.36 0.41

2.5 U 2.5 U 6 2.5 U 2.5 U 2.5 U 

NOTE: All concentrations are in mg/kg
Table A3-3. Results of the metals analyses of sediment samples collected for the WDNR food chain study

Station ID

Sample ID

Chemical

Arsenic

Cadmium

Chromium

Copper

Lead

Mercury

Selenium

Silver

603394 603395 603396

1C 1D 1E 

Conc Q Conc Q Conc Q

0.84 1.27 1.35

0.4 U 0.4 U 0.4 U 

12 19 16

9.6 19 16

7 13 12

0.05 0.12 0.081

0.44 0.73 0.46

2.5 U 2.5 U 2.5 U 

NOTE: All concentrations are in mg/kg
Table A3-5. Results of the PAH analyses of tissue samples collected for the WDNR food chain study

Crayfish

Control Rochester Park Between Kohler Dams Esselingen Park

PAH mg/kg ww mg/kg ww mg/kg ww mg/kg ww

Acenaphthylene 0.0035 0.0035 0.0035 0.0035

Acenapthene 0.0035 0.0035 0.0035 0.0035

Fluorene 0.0035 0.0035 0.0035 0.0035

Phenanthrene 0.0035 0.0035 0.0035 0.0035

Anthracene 0.0035 0.0035 0.0035 0.0035

Fluoranthene 0.0035 0.0035 0.0035 0.0035

Pyrene 0.0035 0.0035 0.0035 0.0035

Benzo(a)anthracene 0.0035 0.0035 0.0035 0.0035

Chrysene 0.0035 0.0035 0.0035 0.0035

Benzo(b)fluoranthene 0.0035 0.0035 0.0035 0.0035

Benzo(k)fluoranthene 0.0035 0.0035 0.0035 0.0035

Benzo(e)pyrene 0.0035 0.0035 0.0035 0.0035

Benzo(a)pyrene 0.0035 0.0035 0.0035 0.0035

Perylene 0.0035 0.0035 0.0035 0.0035

Ideno(1,2,3-cd)pyrene 0.0035 0.0035 0.0035 0.0035

Dibenzo(a,h)anthracene 0.0035 0.0035 0.0035 0.0035

Benzo(g,h,i)perylene 0.0035 0.0035 0.0035 0.0035

Note: 0.0035 is 1/2 the detection limit
Table A3-5. Results of the PAH analyses of tissue samples collected for the WDNR food chain study

PAH

Acenaphthylene

Acenapthene

Fluorene

Phenanthrene

Anthracene

Fluoranthene

Pyrene

Benzo(a)anthracene

Chrysene

Benzo(b)fluoranthene

Benzo(k)fluoranthene

Benzo(e)pyrene

Benzo(a)pyrene

Perylene

Ideno(1,2,3-cd)pyrene

Dibenzo(a,h)anthracene

Benzo(g,h,i)perylene

Note: 0.0035 is 1/2 the detection limit

Larval Insects

Control Rochester Park Between Kohler Dams Esselingen Park

mg/kg ww mg/kg ww mg/kg ww mg/kg ww

0.0035 0.0035 0.0035 0.0035

0.0035 0.0035 0.0035 0.0035

0.0097 0.0112 0.0106 0.0157

0.0035 0.0145 0.0172 0.0382

0.0035 0.0035 0.0035 0.0035

0.0035 0.016 0.007 0.0335

0.0035 0.0126 0.0127 0.0281

0.0035 0.0035 0.0035 0.0035

0.0035 0.0035 0.0035 0.0035

0.0035 0.0072 0.0035 0.0096

0.0035 0.0035 0.0035 0.0035

0.0035 0.0035 0.0035 0.0035

0.0035 0.0035 0.0035 0.0035

0.0035 0.0035 0.0035 0.0035

0.0035 0.0035 0.0035 0.0035

0.0035 0.0035 0.0035 0.0035

0.0035 0.0035 0.0035 0.0099
Table A3-5. Results of the PAH analyses of tissue samples collected for the WDNR food chain study

PAH

Acenaphthylene

Acenapthene

Fluorene

Phenanthrene

Anthracene

Fluoranthene

Pyrene

Benzo(a)anthracene

Chrysene

Benzo(b)fluoranthene

Benzo(k)fluoranthene

Benzo(e)pyrene

Benzo(a)pyrene

Perylene

Ideno(1,2,3-cd)pyrene

Dibenzo(a,h)anthracene

Benzo(g,h,i)perylene

Note: 0.0035 is 1/2 the detection limit

Emergent Insects

Control Rochester Park Between Kohler Dams Esselingen Park

mg/kg ww mg/kg ww mg/kg ww mg/kg ww

0.0035 0.0035 0.0035 0.0035

0.0035 0.0035 0.0035 0.0035

0.014 0.0435 0.010 0.0091

0.0159 0.0171 0.025 0.0351

0.0035 0.0035 0.000 0.0000

0.0035 0.010 0.015 0.0241

0.0035 0.007 0.009 0.0230

0.0035 0.012 0.004 0.0035

0.0035 0.009 0.009 0.0217

0.0035 0.0035 0.0035 0.0035

0.0035 0.0035 0.0035 0.0035

0.0035 0.0035 0.0035 0.0035

0.0035 0.0035 0.0035 0.0035

0.0035 0.0035 0.0035 0.0035

0.0035 0.0035 0.0035 0.0035

0.0035 0.0035 0.0035 0.0080

0.0035 0.0035 0.0035 0.0083
Table A3-6. Results of metals analyses of crayfish and emergent and

larval insect tissue samples collected for the WDNR food chain study

Segment 1, Control

Emergent Insects

Station ID 603384 603384 603384 603384

Sample ID 9401 9402 9403 9404

Chemical Conc Q Conc Q Conc Q Conc Q

Arsenic 0.1 U 0.1 0.1 U 0.1 U

Cadmium 0.01 0.015 0.009 0.012

Chromium 0.2 0.1 0.1 0.1

Copper 7.7 11 9 9.5

Lead 2.8 0.04 0.11 0.08

Mercury 0.048 0.076 0.064 0.056

Selenium 0.64 0.48 0.968 1.33

Silver 0.019 0.024 0.018 0.007

Segment 1, Control

Larval Insects

Station ID 603384 603384 603384 603384

Sample ID 9405 9406 9407 9408

Chemical Conc Q Conc Q Conc Q Conc Q

Arsenic 0.2 0.2 0.2 0.1

Cadmium 0.019 0.024 0.016 0.014

Chromium 0.7 0.8 0.6 0.6

Copper 3.2 3.4 3.2 3.7

Lead 0.57 0.55 0.36 0.35

Mercury 0.03 0.028 0.018 0.022

Selenium 0.48 0.567 0.39 0.511

Silver 0.004 0.01 U 0.011

Segment 1, Control

Crayfish

Station ID 603384 603384 603384

Sample ID 9409 9410 9411

Chemical Conc Q Conc Q Conc Q

Arsenic 0.7 0.6 0.5

Cadmium 0.01 0.013 0.011

Chromium 0.3 0.5 0.3

Copper 18 15 17

Lead 0.14 0.17 0.12

Mercury 0.042 0.038 0.049

Selenium 0.35 0.25 0.27

Silver 0.066 0.08 0.073

NOTE: All concentrations are in mg/kg dry weight
Table A3-6. Continued

Segment 2, Rochester Park

Emergent Insects

Station ID 603385 603385 603385 603385

Sample ID 9401 9402 9403 9404

Chemical Conc Q Conc Q Conc Q Conc Q

Arsenic 0.1 U 0.1 0.1 U 0.2

Cadmium 0.015 0.016 0.008 0.011

Chromium 0.1 0.1 0.1 0.1

Copper 8.2 7.6 7.9 7.6

Lead 0.14 0.05 0.17 0.16

Mercury 0.048 0.053 0.044 0.049

Selenium 1.058 0.899 1.11 1.139

Silver 0.019 0.021 0.017 0.02

Segment 2, Rochester Park

Larval Insects

Station ID 603385 603385 603385 603385

Sample ID 9405 9406 9407 9408

Chemical Conc Q Conc Q Conc Q Conc Q

Arsenic 0.1 0.2 0.2 0.2

Cadmium 0.016 0.017 0.018 0.013

Chromium 0.8 0.7 0.9 0.6

Copper 2.8 3.9 3.7 5.2

Lead 0.84 0.73 0.79 0.59

Mercury 0.026 0.026 0.01

Selenium 0.426 0.581 0.448 0.358

Silver 0.009 0.011 0.011 0.002

Segment 2, Rochester Park

Crayfish

Station ID 603385 603385 603385

Sample ID 9409 9410 9411

Chemical Conc Q Conc Q Conc Q

Arsenic 0.5 0.6 0.4

Cadmium 0.01 0.007 0.007

Chromium 0.3 0.3 0.3

Copper 24 24 23

Lead 0.18 0.23 0.25

Mercury 0.017 0.024 0.02

Selenium 0.253 0.61 0.311

Silver 0.065 0.07 0.065

NOTE: All concentrations are in mg/kg dry weight
Table A3-6. Continued

Segment 3, Between Kohler Dams

Emergent Insects

Station ID 603386 603386 603386 603386

Sample ID 9401 9402 9403 9404

Chemical Conc Q Conc Q Conc Q Conc Q

Arsenic 0.1 U 0.1 U 0.1 U 0.1 U

Cadmium 0.006 0.014 0.012 0.011

Chromium 0.2 0.1 0.1 0.1

Copper 8.3 7.8 6.7 6.4

Lead 0.2 0.43 0.45 0.19

Mercury 0.031 0.035 0.031 0.034

Selenium 1.039 0.965 0.964 1.314

Silver 0.01 U 0.01 U 0.01 U 0.01 U

Segment 3, Between Kohler Dams

Larval Insects

Station ID 603386 603386 603386 603386

Sample ID 9405 9406 9407 9408

Chemical Conc Q Conc Q Conc Q Conc Q

Arsenic 0.2 0.1 0.1 0.1

Cadmium 0.015 0.014 0.015 0.01

Chromium 0.7 0.7 0.9 0.7

Copper 4 4.1 3.9 3.9

Lead 0.65 0.63 0.49 0.42

Mercury 0.02 0.025 0.018 0.017

Selenium 0.332 0.399 0.332 0.191

Silver 0.01 U 0.01 U 0.01 U 0.01 U

Segment 3, Between Kohler Dams

Crayfish

Station ID 603386 603386 603386

Sample ID 9409 9410 9411

Chemical Conc Q Conc Q Conc Q

Arsenic 0.5 0.5 0.6

Cadmium 0.007 0.008 0.009

Chromium 0.7 0.4 0.4

Copper 23 22 23

Lead 0.26 0.26 0.3

Mercury 0.022 0.028 0.023

Selenium 0.355 0.333 0.41

Silver 0.05 0.06 0.05

NOTE: All concentrations are in mg/kg dry weight
Table A3-6. Continued

Segment 5, Esslingen Park

Emergent Insects

Station ID 603387 603387 603387 603387

Sample ID 9401 9402 9403 9404

Chemical Conc Q Conc Q Conc Q Conc Q

Arsenic 0.1 U 0.1 U 0.1 0.1 U

Cadmium 0.027 0.014 0.013 0.012

Chromium 0.1 0.1 0.2 0.2

Copper 7.7 8.9 9 9.5

Lead 0.3 0.31 0.31 0.33

Mercury 0.048 0.039 0.035 0.027

Selenium 0.909 1.067 1.283 1.177

Silver 0.01 0.01 U 0.01 U 0.01 U

Segment 5, Esslingen Park

Larval Insects

Station ID 603387 603387 603387 603387

Sample ID 9405 9406 9407 9408

Chemical Conc Q Conc Q Conc Q Conc Q

Arsenic 0.1 U 0.1 U 0.1 0.1

Cadmium 0.016 0.017 0.013 0.14

Chromium 0.8 1.3 0.9 0.8

Copper 4 4.8 5.2 5.4

Lead 1 1.5 0.88 0.9

Mercury 0.019 0.018 0.03 0.015

Selenium 0.32 0.482 0.347 0.49

Silver 0.01 U 0.01 U 0.01 U 0.01 U

Segment 5, Esslingen Park

Crayfish

Station ID 603387 603387 603387

Sample ID 9409 9410 9411

Chemical Conc Q Conc Q Conc Q

Arsenic 0.6 0.5 0.6

Cadmium 0.011 0.01 0.01

Chromium 0.4 0.3 0.3

Copper 29 28 29

Lead 0.48 0.41 0.46

Mercury 0.028 0.028 0.03

Selenium 0.43 0.215 0.388

Silver 0.03 0.03 0.03

NOTE: All concentrations are in mg/kg dry weight
Table A3-7. Results of the PCB congener analyses of small mammal tissue samples collected for the WDNR food chain study

Genus WDNR ID Weight (g) Sex Lipids PCB026 PCB028 PCB052 PCB049 PCB047 PCB044 PCB041 PCB074

(%)

Clethrinomys 94088 22 F 1.3 2.5 U 5.6 1 U 0.6 U 2.6 1.1 U 2 U 17

Clethrinomys 94086 46 F 2.5 2.5 U 2.5 U 1 U 0.6 U 1.2 U 1.1 U 2 U 1 U

Clethrinomys 94087 46 F 2.5 2.5 U 2.5 U 1 U 0.6 U 1.2 U 1.1 U 2 U 1 U

Microtus 94090 28 M 2.3 2.5 U 14 1.4 0.87 10 1.1 U 2 U 12

Microtus 94091 13 1.8 2.5 U 2.5 U 1 U 0.6 U 1.2 U 1.1 U 2 U 1 U

Microtus 94100 22 M 2.8 2.5 U 2.5 U 1 U 0.6 U 1.2 U 1.1 U 2 U 1 U

Microtus 94101 43 F 4.7 0.96 110 8.1 5 50 1.4 11 53

Microtus 94089 51 F 2.1 2.5 U 2.5 U 1 U 0.6 U 1.2 U 1.1 U 2 U 1 U

Peromyscus 94092 27 F 4.2 2.5 U 12 4.9 2.1 34 1.1 U 3.4 12

Peromyscus 94093 26 M 4.1 2.5 U 24 2.8 0.78 33 1.1 U 2.7 29

Peromyscus 94094 18 M 2.4 2.5 U 13 1.5 0.6 U 17 1.1 U 2 U 16

Peromyscus 94095 22 M 3.1 2.5 U 2.5 U 1 U 0.6 U 4.8 1.1 U 2 U 3.4

Peromyscus 94104 20 M 2.2 2.5 U 2.5 U 1 U 0.6 U 1.2 U 1.1 U 2 U 1 U

Peromyscus 94103 18 M 3.5 2.5 U 2.5 U 1 U 0.6 U 1.5 1.1 U 2 U 3.1

Peromyscus 94102 22 M 0.8 2.5 U 2.5 U 1 U 0.6 U 6.3 1.1 U 2 U 1.4

Tamias 94099 94 F 1.7 2.5 U 2.5 U 1 U 0.6 U 2 1.1 U 2 U 1.7

Tamias 94098 88 M 1.3 2.5 U 2.5 U 1 U 0.6 U 1.2 U 1.1 U 2 U 1 U

Tamias 94106 106 F 2.5 2.5 U 18 2 1.3 27 1.1 U 2 U 30

Tamias 94105 51 M 1 2.5 U 5.4 1 U 0.6 U 8.7 1.1 U 2 U 36

Zapus 94096 13 M 1.6 2.5 U 2.5 U 1 U 0.6 U 2 1.1 U 2 U 1 U

Zapus 94097 23 F 3.1 2.5 U 2.5 U 1 U 0.6 U 1.2 U 1.1 U 2 U 1 U

NOTE: All PCB concentrations are in μg/kg.
Table A3-7. Results of the PCB congener analyses of small mammal tissue samples collected for the WDNR food chain study

Genus WDNR ID

Clethrinomys 94088

Clethrinomys 94086

Clethrinomys 94087

Microtus 94090

Microtus 94091

Microtus 94100

Microtus 94101

Microtus 94089

Peromyscus 94092

Peromyscus 94093

Peromyscus 94094

Peromyscus 94095

Peromyscus 94104

Peromyscus 94103

Peromyscus 94102

Tamias 94099

Tamias 94098

Tamias 94106

Tamias 94105

Zapus 94096

Zapus 94097

PCB070 PCB066 PCB056 PCB084 PCB101 PCB099 PCB097 PCB087 PCB085

2.4 U 13 3.6 1.3 U 0.91 2.1 0.8 U 1 U 1 U

2.4 U 2.8 U 1.8 U 1.3 U 0.6 U 0.8 U 1 U 1 U

2.4 U 2.8 U 1.8 U 1.3 U 0.6 U 0.8 U 1 U 1 U

6.2 34 8.7 1.3 U 6.1 21 1 1.5 1 U

2.4 U 2.8 U 1.8 U 1.3 U 0.6 U 0.8 U 1 U 1 U

2.4 U 2.8 U 1.8 U 1.3 U 0.6 U 0.8 U 1 U 1 U

48 140 47 1.3 U 15 32 2.7 7 13

2.4 U 2.8 U 1.8 U 1.3 U 0.6 U 0.8 U 1 U 1 U

2.4 U 20 6.2 4.2 5.7 63 0.8 U 2.5 8.8

2.4 U 24 11 1.3 U 6.8 45 0.8 U 2.3 7.1

2.4 U 13 5.7 1.3 U 3.6 24 0.8 U 1.2 3.8

2.4 U 2.8 1.8 U 1.3 U 1.3 9.7 0.8 U 1 U 1 U

2.4 U 2.8 U 1.8 U 1.3 U 0.6 U 0.8 U 1 U 1 U

2.4 U 3.2 1.8 U 1.3 U 1.3 4.8 0.8 U 1 U 1 U

2.4 U 2.8 U 1.8 U 1.3 U 1.1 19 0.8 U 1 U 2.4

2.4 U 4.5 1.8 U 1.3 U 0.6 U 6.9 0.8 U 1 U 1 U

2.4 U 2.8 U 1.8 U 1.3 U 0.6 U 0.8 U 1 U 1 U

3.5 35 16 1.3 U 2.5 25 0.8 U 2.1 8.7

2.4 U 6.1 11 1.3 U 0.6 U 27 0.8 U 1.9 4

2.4 U 2.8 U 1.8 U 1.3 U 0.7 2.3 0.8 U 1 U 1 U

2.4 U 2.8 U 1.8 U 1.3 U 0.6 U 0.8 U 1 U 1 U

NOTE: All PCB concentrations are in μg/kg.
Table A3-7. Results of the PCB congener analyses of small mammal tissue samples collected for the WDNR food chain study

Genus WDNR ID

Clethrinomys 94088

Clethrinomys 94086

Clethrinomys 94087

Microtus 94090

Microtus 94091

Microtus 94100

Microtus 94101

Microtus 94089

Peromyscus 94092

Peromyscus 94093

Peromyscus 94094

Peromyscus 94095

Peromyscus 94104

Peromyscus 94103

Peromyscus 94102

Tamias 94099

Tamias 94098

Tamias 94106

Tamias 94105

Zapus 94096

Zapus 94097

PCB077 PCB149 PCB118 PCB146 PCB132 PCB138 PCB178 PCB182 PCB183

1.3 U 1.1 U 51 5.2 1.9 17 1.3 4.1 1.8 U

1.3 U 1.1 U 1 U 1.3 U 1.5 U 1.8 U

1.3 U 1.1 U 1 U 1.3 U 1.5 U 1.8 U

4.5 1.6 52 6.7 27 35 1.3 U 3.8 1.8 U

1.3 U 1.1 U 1 U 1.3 U 1.5 U 1.8 U

1.3 U 1.1 U 1 U 1.3 U 1.5 U 1.8 U

13 1.1 U 97 8.3 16 38 1.3 U 4.4 1.8 U

1.3 U 1.1 U 1 U 1.3 U 1.5 U 1.8 U

2.7 1.6 33 19 180 140 3.2 23 4.7

1.9 1.2 120 22 180 140 4.8 32 5.5

1.3 U 1.1 U 61 11 92 72 2.5 16 2.8

1.3 U 1.1 U 20 6.3 110 71 1.5 26 3.7

1.3 U 1.1 U 1 U 1.3 U 1.5 U 1.8 U

1.3 U 1.1 U 28 7.7 43 34 1.8 14 1.8 U

1.3 U 1.1 U 19 14 160 120 3.6 33 5.1

1.3 U 1.1 U 10 2.6 28 20 1.3 U 2.4 1.8 U

1.3 U 1.1 U 1 U 1.3 U 1.5 U 1.8 U

2.4 1.1 U 37 1 U 27 21 1.3 U 1.5 U 1.8 U

1.3 U 1.1 U 59 2.3 42 33 1.3 U 1.9 1.8 U

1.3 U 1.1 U 18 9.9 110 67 1.4 23 3.1

1.3 U 1.1 U 1 U 1.3 U 1.5 U 1.8 U

NOTE: All PCB concentrations are in μg/kg.
Table A3-7. Results of the PCB congener analyses of small mammal tissue samples collected for the WDNR food chain study

Genus WDNR ID

Clethrinomys 94088

Clethrinomys 94086

Clethrinomys 94087

Microtus 94090

Microtus 94091

Microtus 94100

Microtus 94101

Microtus 94089

Peromyscus 94092

Peromyscus 94093

Peromyscus 94094

Peromyscus 94095

Peromyscus 94104

Peromyscus 94103

Peromyscus 94102

Tamias 94099

Tamias 94098

Tamias 94106

Tamias 94105

Zapus 94096

Zapus 94097

PCB177 PCB172 PCB180 PCB170 PCB201 PCB196 PCB194 PCB206

1.8 1.8 U 7.8 8.1 2.3 3 U 1.3 1.5 U

1.1 U 1.8 U 2.2 U 2.5 U 1.8 U 3 U 1 U 1.5 U

1.1 U 1.8 U 2.2 U 2.5 U 1.8 U 3 U 1 U 1.5 U

2.4 1.8 U 6.5 9.1 1.8 U 3 U 1 U 1.5 U

1.1 U 1.8 U 2.2 U 2.5 U 1.8 U 3 U 1 U 1.5 U

1.1 U 1.8 U 2.2 U 2.5 U 1.8 U 3 U 1 U 1.5 U

2.2 1.8 U 8 9.5 2.1 3 U 1.1 1.5 U

1.1 U 1.8 U 2.2 U 2.5 U 1.8 U 3 U 1 U 1.5 U

1.2 5 46 46 9.2 17 7.8 4.5

2.6 5.1 59 45 9.7 17 9.2 4.4

1.3 2.7 31 23 4.9 8.3 4.7 2.3

1.1 U 3.4 63 42 12 23 9.3 4.7

1.1 U 1.8 U 2.2 U 2.5 U 1.8 U 3 U 1 U 1.5 U

1.1 U 2.1 12 9.2 3.3 3.1 1.9 1.5 U

1.1 5 68 51 14 22 11 5.4

1.1 U 1.8 U 10 10 1.8 U 3.3 2.4 1.7

1.1 U 1.8 U 2.2 U 2.5 U 1.8 U 3 U 1 U 1.5 U

1.1 U 1.8 U 5.7 6.5 1.8 U 3 U 1 U 1.5 U

1.1 U 1.8 U 7 7.6 1.8 U 3 U 1.1 1.5 U

1.1 U 1.8 U 59 36 3.8 15 6.9 2.5

1.1 U 1.8 U 2.2 U 2.5 U 1.8 U 3 U 1 U 1.5 U

NOTE: All PCB concentrations are in μg/kg.
Table A3-7. Results of the PCB congener analyses of small mammal tissue samples collected for the WDNR food chain study

Genus WDNR ID

Clethrinomys 94088

Clethrinomys 94086

Clethrinomys 94087

Microtus 94090

Microtus 94091

Microtus 94100

Microtus 94101

Microtus 94089

Peromyscus 94092

Peromyscus 94093

Peromyscus 94094

Peromyscus 94095

Peromyscus 94104

Peromyscus 94103

Peromyscus 94102

Tamias 94099

Tamias 94098

Tamias 94106

Tamias 94105

Zapus 94096

Zapus 94097

SUM (detected values only) Total of first 17 Total of next 17 Total of all 34

Congeners Congeners congeners**

170.81 51.66 107.05 158.71

46.5 11.8 11.45 23.25

46.5 11.8 11.45 23.25

285.47 120.72 154.7 275.42

46.5 11.8 11.45 23.25

46.5 11.8 11.45 23.25

755.56 544.81 204.85 749.66

46.5 11.8 11.45 23.25

729.5 182.2 543.9 726.1

855.98 192.53 659.4 851.93

447.4 104.15 336.7 440.85

439.4 31 397.65 428.65

46.5 11.8 11.45 23.25

198.8 22.9 163.5 186.4

584.6 40.1 533.4 573.5

134.3 24.4 95.5 119.9

46.5 11.8 11.45 23.25

295.3 174.95 108.05 283

281 106.25 161.25 267.5

387.7 15.9 358.25 374.15

11.8 11.45 23.25

note this simply ** Detected conc.+ 1/2 undetected only if congener detected in another sample.

adds the columns

NOTE: All PCB concentrations are in μg/kg.
APPENDIX B

Toxic Equivalent Calculations
APPENDIX B-1

Toxic Equivalent Concentrations for Fish
LIST OF TABLES

Table B1-1. Fish TEQs using 1997 ERA fish data

Table B1-2. Fish TEQs using fish tissue samples collected for the WDNR food

chain study
APPENDIX B-1

DEFINITION OF TERMS

Definition of qualifiers

> - sample over linear range of method

D - diluted sample

L - labeled compound

U - undetected 

U*I - significant interference present; value represents operator's best judgement of reporting limit

1997 ERA sample identification 

F1-1

fish tissue sample river segment sample number
Table B1-1. Fish TEQs using 1997 ERA fish data

Station F1-1 Percent of F1-2 Percent of F1-3 Percent of 

Chemical Fish TEFs TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

PCB077 0.00016 1.71E-05 13.88% 1.84E-05 13.33% 1.60E-05 10.23%

PCB105 0.00000172 4.13E-06 3.35% 4.30E-06 3.11% 3.96E-06 2.53%

PCB114 0.000005 9.30E-07 L 0.75% 8.15E-07 L 0.59% 1.39E-06 0.89%

PCB118 0.00000302 2.36E-05 19.10% 2.39E-05 17.28% 2.27E-05 14.48%

PCB123 0.000005 4.44E-07 0.36% 3.61E-07 0.26% 7.60E-07 0.49%

PCB126 0.005 7.20E-05 58.37% 8.45E-05 61.21% 1.06E-04 67.77%

PCB156 0.00000167 1.60E-06 1.30% 1.69E-06 1.22% 1.46E-06 0.93%

PCB157 0.000005 8.35E-07 0.68% 9.30E-07 0.67% 1.09E-06 0.69%

PCB167 0.000005 2.48E-06 2.01% 2.87E-06 2.08% 2.77E-06 1.77%

PCB189 0.000005 2.62E-07 0.21% 3.29E-07 0.24% 3.44E-07 0.22%

Total TEQ 1.23E-04 1.38E-04 1.56E-04

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was 

 detected in another sample. This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.
Table B1-1. Continued

Station

Chemical

PCB077

PCB105

PCB114

PCB118

PCB123

PCB126

PCB156

PCB157

PCB167

PCB189

Total TEQ

F2-1 Percent of F2-2 Percent of F2-3 Percent of 

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

6.30E-03 D 43.97% 8.48E-03 D 42.89% 4.06E-03 39.91%

5.16E-04 >D 3.60% 5.68E-04 D 2.87% 3.61E-04 >D 3.55%

1.44E-04 1.00% 1.64E-04 0.83% 1.20E-04 1.18%

1.72E-03 >D 12.01% 2.69E-03 >D 13.59% 1.45E-03 >D 14.24%

5.85E-05 0.41% 5.50E-05 0.28% 5.45E-05 0.54%

5.35E-03 37.31% 7.55E-03 38.18% 3.93E-03 38.59%

8.58E-05 0.60% 1.05E-04 0.53% 7.53E-05 0.74%

5.45E-05 0.38% 5.25E-05 0.27% 4.08E-05 0.40%

9.35E-05 0.65% 9.95E-05 0.50% 7.85E-05 0.77%

1.08E-05 0.08% 1.18E-05 0.06% 9.00E-06 0.09%

1.43E-02 1.98E-02 1.02E-02

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was 

 detected in another sample. This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.
Table B1-1. Continued

Station

Chemical

PCB077

PCB105

PCB114

PCB118

PCB123

PCB126

PCB156

PCB157

PCB167

PCB189

Total TEQ

F3-1 Percent of F3-2 Percent of F3-3 Percent of 

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

5.63E-03 D 30.98% 3.90E-03 27.05% 4.26E-03 D 27.41%

6.71E-04 3.69% 6.02E-04 4.17% 5.68E-04 3.66%

1.73E-04 0.95% 1.67E-04 1.15% 1.45E-04 0.93%

3.32E-03 18.27% 2.93E-03 20.30% 2.93E-03 18.86%

6.60E-05 0.36% 6.95E-05 0.48% 5.75E-05 0.37%

7.95E-03 43.72% 6.45E-03 44.69% 7.25E-03 46.69%

1.30E-04 0.71% 1.13E-04 0.78% 1.26E-04 0.81%

7.00E-05 0.38% 7.25E-05 0.50% 6.85E-05 0.44%

1.54E-04 0.85% 1.11E-04 0.77% 1.14E-04 0.73%

1.50E-05 0.08% 1.42E-05 0.10% 1.59E-05 0.10%

1.82E-02 1.44E-02 1.55E-02

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was 

 detected in another sample. This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.
Table B1-1. Continued

Station

Chemical

PCB077

PCB105

PCB114

PCB118

PCB123

PCB126

PCB156

PCB157

PCB167

PCB189

Total TEQ

F5-1 Percent of F5-2 Percent of F5-3 Percent of 

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

1.65E-03 30.70% 1.65E-03 26.50% 1.68E-03 25.34%

2.41E-04 4.49% 2.75E-04 4.43% 3.10E-04 4.67%

6.95E-05 1.29% 8.70E-05 1.40% 9.90E-05 1.49%

1.27E-03 23.63% 1.39E-03 22.34% 1.57E-03 23.68%

3.09E-05 0.57% 4.04E-05 0.65% 3.26E-05 0.49%

1.97E-03 36.70% 2.61E-03 41.97% 2.77E-03 41.77%

5.63E-05 1.05% 5.95E-05 0.96% 6.21E-05 0.94%

2.59E-05 0.48% 3.15E-05 0.51% 3.32E-05 0.50%

5.25E-05 0.98% 7.00E-05 1.13% 6.65E-05 1.00%

5.85E-06 0.11% 8.35E-06 0.13% 7.65E-06 0.12%

5.37E-03 6.22E-03 6.63E-03

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was 

 detected in another sample. This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.
Table B1-2. Fish TEQs using fish tissue samples collected for the WDNR food chain study 

Station 603388 603388 603388

Sample 9413 9414 9415

Species longnose dace longnose dace longnose dace 

Age class

Location control control control 

Percent of Percent of Percent of

Chemical Fish TEFs TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

PCB077 0.00016 0.00008 U 2.98% na na

PCB105 0.00000172 1.7E-05 0.63% na na

PCB118 0.00000302 7.55E-05 2.81% 2.05E-05 na 9.97E-06 na

PCB126 0.005 0.0025 U 93.16% na na

PCB156 0.00000167 4.01E-06 0.15% na na

PCB157 0.000005 2.5E-06 U 0.09% na na

PCB167 0.000005 4.5E-06 U 0.17% 4.5E-06 U na 4.5E-06 U na

Total TEQ 0.002684 na na

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603389 603389 603389

9413 9414 9415

longnose dace longnose dace longnose dace 

rochester park rochester park rochester park 

Percent of Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

na na 0.00256 24.54%

na na 0.000413 3.96%

0.000906 na 0.000966 na 0.002235 21.42%

na na 0.005 U*I 47.93%

na na 7.35E-05 0.70%

na na 0.000055 0.53%

0.000055 na 0.00006 na 0.000095 0.91%

na na 0.010431

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603390

9413

longnose dace 

esselingen park 

Percent of

TEQ Q Total TEQ

0.0032 36.35%

0.000447 5.08%

0.002295 26.07%

0.0025 U 28.40%

9.02E-05 1.02%

0.00013 1.48%

0.00014 1.59%

0.008803

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.

603390 603390

9414 9415

longnose dace longnose dace 

esselingen park esselingen park 

Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ

na na

na na

0.002114 na 0.001389 na

na na

na na

na na

0.00011 na 0.00007 na

na na

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603391 603391 603391

9413 9414 9415

longnose dace longnose dace longnose dace 

between kohler dams between kohler dams between kohler dams

Percent of Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

na na 0.00272 36.40%

na na 0.0002924 3.91%

0.0013892 na 0.0016912 na 0.0016912 22.63%

na na 0.0025 U 33.46%

na na 0.00006346 0.85%

na na 0.000095 1.27%

0.00008 na 0.000095 na 0.00011 1.47%

na na 0.00747206

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603388 603388 603388

9401 9402 9403

smallmouth bass smallmouth bass smallmouth bass 

adult adult adult

control control control 

Percent of Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.000192 6.77% 0.00008 U 3.03% 0.00008 U 3.06%

1.48E-05 0.52% 7.74E-06 0.29% 5.33E-06 0.20%

0.000118 4.16% 3.93E-05 1.49% 2.45E-05 0.93%

0.0025 U 88.21% 0.0025 U 94.83% 0.0025 U 95.47%

2.67E-06 0.09% 2.34E-06 0.09% 1.84E-06 0.07%

2.5E-06 U 0.09% 2.5E-06 U 0.09% 2.5E-06 U 0.10%

4.5E-06 U 0.16% 4.5E-06 U 0.17% 4.5E-06 U 0.17%

0.002834 0.002636 0.002619

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603388 603388 603388

9404 9405 9406

smallmouth bass smallmouth bass smallmouth bass 

young of year young of year young of year

control control control 

Percent of Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

na na 0.00008 U 3.06%

na na 2.41E-06 0.09%

1.18E-05 na 1.33E-05 na 2.3E-05 0.88%

na na 0.0025 U 95.67%

na na 8.35E-07 U 0.03%

na na 2.5E-06 U 0.10%

4.5E-06 U na 4.5E-06 U na 4.5E-06 U 0.17%

na na 0.002613

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603389 603389 603389

9401 9402 9403

smallmouth bass smallmouth bass smallmouth bass 

adult adult adult

rochester park rochester park rochester park 

Percent of Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.01568 30.78% 0.01376 32.74% 0.00768 36.10%

0.001324 2.60% 0.001582 3.77% 0.000791 3.72%

0.00604 11.86% 0.005738 13.65% 0.003322 15.62%

0.027 53.01% 0.02 47.59% 0.009 42.31%

0.000284 0.56% 0.000317 0.76% 0.00015 0.71%

0.00026 0.51% 0.00027 0.64% 0.000135 0.63%

0.00035 0.69% 0.000355 0.84% 0.000195 0.92%

0.050938 0.042023 0.021274

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603389 603389 603389

9404 9405 9406

smallmouth bass smallmouth bass smallmouth bass 

young of year young of year young of year

rochester park rochester park rochester park 

Percent of Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

na na 0.00464 32.46%

na na 0.000585 4.09%

0.002778 na 0.002174 na 0.002778 19.44%

na na 0.006 U*I 41.98%

na na 9.02E-05 0.63%

na na 0.00008 0.56%

0.00016 na 0.00019 na 0.00012 0.84%

na na 0.014293

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603390 603390 603390

9401 9402 9403

smallmouth bass smallmouth bass smallmouth bass 

adult adult adult

esselingen park esselingen park esselingen park 

Percent of Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.00272 32.10% 0.00256 27.72% 0.00208 23.13%

0.000361 4.26% 0.00043 4.66% 0.000413 4.59%

0.002174 25.66% 0.002476 26.82% 0.002476 27.53%

0.003 U*I 35.40% 0.0035 U*I 37.90% 0.00375 U*I 41.69%

6.85E-05 0.81% 8.85E-05 0.96% 8.52E-05 0.95%

0.00005 0.59% 0.000055 0.60% 0.000055 0.61%

0.0001 1.18% 0.000125 1.35% 0.000135 1.50%

0.008474 0.009235 0.008994

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603390 603390 603390

9404 9405 9406

smallmouth bass smallmouth bass smallmouth bass 

young of year young of year young of year

esselingen park esselingen park esselingen park 

Percent of Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

na na 2.24E-03 33.84%

na na 2.92E-04 4.42%

0.000755 na 1.09E-03 na 1.45E-03 21.90%

na na 2.50E-03 U 37.77%

na na 3.84E-05 0.58%

na na 3.40E-05 0.51%

4.65E-05 na 6.50E-05 na 6.50E-05 0.98%

na na 6.62E-03

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603391 603391 603391

9401 9402 9403

smallmouth bass smallmouth bass smallmouth bass 

adult adult adult

between kohler dams between kohler dams between kohler dams

Percent of Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

5.76E-03 26.62% 0.00416 32.00% 0.00672 27.49%

5.85E-04 2.70% 0.0003612 2.78% 0.0007912 3.24%

3.62E-03 16.75% 0.0020536 15.79% 0.004228 17.29%

1.10E-02 50.83% 0.006 46.15% 0.012 49.08%

1.47E-04 0.68% 0.00010688 0.82% 0.0002004 0.82%

2.45E-04 1.13% 0.00015 1.15% 0.00018 0.74%

2.80E-04 1.29% 0.00017 1.31% 0.00033 1.35%

2.16E-02 0.01300168 0.0244496

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603391 603391 603391

9404 9405 9406

smallmouth bass smallmouth bass smallmouth bass 

young of year young of year young of year

between kohler dams between kohler dams between kohler dams

Percent of Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

na na 0.00656 30.43%

na na 0.0007396 3.43%

0.0025066 na 0.0021744 na 0.003624 16.81%

na na 0.01 46.38%

na na 0.00016533 0.77%

na na 0.00018 0.83%

0.000135 na 0.00012 na 0.00029 1.35%

na na 0.02155893

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603388 603388 603388

9407 9408 9409

white sucker white sucker white sucker 

adult adult adult

control control control 

Percent of Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.00008 U 3.06% na na

4.82E-06 0.18% na na

2.36E-05 0.90% 6.34E-06 na 3.62E-06 na

0.0025 U 95.56% na na

8.35E-07 U 0.03% na na

2.5E-06 U 0.10% na na

4.5E-06 U 0.17% 4.5E-06 U na 4.5E-06 U na

0.002616 na na

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603388 603388 603388

9410 9411 9412

white sucker white sucker white sucker 

young of year young of year young of year

control control control 

Percent of Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

na na 0.00008 U 3.05%

na na 5.5E-06 0.21%

3.93E-06 na 4.83E-06 na 3.32E-05 1.26%

na na 0.0025 U 95.18%

na na 8.35E-07 U 0.03%

na na 2.5E-06 U 0.10%

4.5E-06 U na 4.5E-06 U na 4.5E-06 U 0.17%

na na 0.002627

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603389 603389 603389

9407 9408 9409

white sucker white sucker white sucker 

adult adult adult

rochester park rochester park rochester park 

Percent of Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.00064 12.87% na na

0.000224 4.50% na na

0.000966 19.44% 0.001419 na 0.002205 na

0.003 U*I 60.34% na na

4.51E-05 0.91% na na

0.000032 0.64% na na

0.000065 1.31% 0.00008 na 0.00012 na

0.004972 na na

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603389 603389 603389

9410 9411 9412

white sucker white sucker white sucker 

young of year young of year young of year

rochester park rochester park rochester park 

Percent of Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

na na 0.00176 22.60%

na na 0.000224 2.87%

0.001117 na 0.001208 na 0.000936 12.02%

na na 0.00475 U*I 61.00%

na na 3.67E-05 0.47%

na na 0.00003 0.39%

0.000065 na 0.000065 na 0.00005 0.64%

na na 0.007787

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603390 603390 603390

9407 9408 9409

white sucker white sucker white sucker 

adult adult adult

esselingen park esselingen park esselingen park 

Percent of Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.000368 10.78% na na

6.88E-05 2.01% na na

0.000423 12.38% 0.001661 na 0.000604 na

0.0025 U 73.22% na na

1.84E-05 0.54% na na

0.000013 0.38% na na

2.35E-05 0.69% 0.0001 na 4.05E-05 na

0.003414 na na

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603390 603390 603390

9410 9411 9412

white sucker white sucker white sucker 

young of year young of year young of year

esselingen park esselingen park esselingen park 

Percent of Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

na na 0.001024 23.74%

na na 0.000105 2.43%

0.000423 na 0.000423 na 0.000604 14.00%

na na 0.0025 U 57.96%

na na 2.17E-05 0.50%

na na 2.75E-05 0.64%

0.000027 na 2.15E-05 na 0.000031 0.72%

na na 0.004313

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603391 603391 603391

9407 9408 9409

white sucker white sucker white sucker 

adult adult adult

between kohler dams between kohler dams between kohler dams

Percent of Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.000896 18.88% na na

0.0002064 4.35% na na

0.0009966 21.00% 0.0014194 na 0.0016308 na

0.0025 U 52.68% na na

0.00003674 0.77% na na

0.00005 1.05% na na

0.00006 1.26% 0.000075 na 0.000085 na

0.00474574 na na

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B1-2. Continued

Station

Sample

Species

Age class

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

PCB167

Total TEQ

603391 603391 603391

9410 9411 9412

white sucker white sucker white sucker 

young of year young of year young of year

between kohler dams between kohler dams between kohler dams

Percent of Percent of Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

na na 0.001248 26.08%

na na 0.00014276 2.98%

0.000755 na 0.001359 na 0.0007852 16.41%

na na 0.0025 U 52.23%

na na 0.00003173 0.66%

na na 0.000036 0.75%

0.0000375 na 0.00006 na 0.0000425 0.89%

na na 0.00478619

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congeners 114, 189, and 81 were not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, and 156 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
APPENDIX B-2

Toxic Equivalent Concentrations for Birds
LIST OF TABLES

Table B2-1. Bird TEQs using 1997 ERA fish data

Table B2-2. Bird TEQs using 1997 ERA sediment data

Table B2-3. Bird TEQs using juvenile fish tissue samples collected for the

WDNR food chain study

Table B2-4. Bird TEQs using crayfish samples collected for the WDNR food

chain study

Table B2-5. Bird TEQs using sediment samples collected for the WDNR food

chain study
APPENDIX B-2

DEFINITION OF TERMS

Definition of qualifiers

> - sample over linear range of method

D - diluted sample

L - labeled compound

*Q - unexplainable result

R - peak detected but did not meet quantification criteria

U - undetected 

U*I - significant interference present; value represents operator's best judgement of reporting limit

1997 ERA sample identification 

S1-1

sediment sample river segment sample number

F1-1

fish tissue sample river segment sample number
Table B2-1. Bird TEQs using 1997 ERA fish data

Station F1-1 Percent of F1-2 Percent of F1-3 Percent of F2-1 Percent of

Chemical Bird TEFs TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

PCB066 0.002 3.00E-03 8.24% 2.80E-03 7.35% 2.80E-03 7.52% 9.80E-01 >D 19.76%

PCB077 0.03 3.21E-03 8.82% 3.45E-03 9.05% 3.00E-03 8.06% 1.18E+00 D 23.83%

PCB105 0.005 1.20E-02 32.96% 1.25E-02 32.80% 1.15E-02 30.89% 1.50E+00 >D 30.24%

PCB110 0.00005 5.00E-04 1.37% 4.35E-04 1.14% 4.75E-04 1.28% 1.40E-01 >D 2.82%

PCB118 0.001 7.80E-03 21.42% 7.90E-03 20.73% 7.50E-03 20.15% 5.70E-01 >D 11.49%

PCB126 0.3 4.32E-03 11.86% 5.07E-03 13.30% 6.36E-03 17.09% 3.21E-01 6.47%

PCB128 0.001 2.60E-03 7.14% 2.60E-03 6.82% 2.50E-03 6.72% 1.30E-01 D 2.62%

PCB156 0.001 9.58E-04 2.63% 1.01E-03 2.65% 8.74E-04 2.35% 5.14E-02 1.04%

PCB157 0.002 3.34E-04 0.92% 3.72E-04 0.98% 4.34E-04 1.17% 2.18E-02 0.44%

PCB167 0.002 9.90E-04 2.72% 1.15E-03 3.01% 1.11E-03 2.97% 3.74E-02 0.75%

PCB170 0.0002 1.81E-04 0.50% 2.50E-04 0.66% 1.84E-04 0.49% 9.64E-03 0.19%

PCB180 0.0002 5.04E-04 1.38% 5.64E-04 1.48% 4.76E-04 1.28% 1.65E-02 0.33%

PCB194 0.00005 1.25E-05 R 0.03% 1.65E-05 0.04% 1.55E-05 0.04% 7.00E-04 D 0.01%

Total TEQ 3.64E-02 3.81E-02 3.72E-02 4.96E+00

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163/164, so this value was not included in total TEQ sum.

3) PCB congener 169 was not detected in any samples, so was not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.
Table B2-1. Continued

F2-2 Percent of F2-3 Percent of F3-1 Percent of F3-2 Percent of F3-3 Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

1.56E+00 >D 23.64% 8.40E-01 >D 22.79% 1.74E+00 25.75% 1.50E+00 26.06% 1.48E+00 25.73%

1.59E+00 D 24.09% 7.62E-01 20.67% 1.06E+00 D 15.63% 7.32E-01 12.72% 7.98E-01 D 13.87%

1.65E+00 D 25.00% 1.05E+00 >D 28.49% 1.95E+00 28.86% 1.75E+00 30.40% 1.65E+00 28.69%

2.10E-01 >D 3.18% 1.15E-01 >D 3.12% 8.50E-02 1.26% 7.50E-02 1.30% 7.00E-02 1.22%

8.90E-01 >D 13.49% 4.80E-01 >D 13.02% 1.10E+00 16.28% 9.70E-01 16.85% 9.70E-01 16.86%

4.53E-01 6.86% 2.36E-01 6.40% 4.77E-01 7.06% 3.87E-01 6.72% 4.35E-01 7.56%

9.80E-02 D 1.48% 8.80E-02 D 2.39% 1.50E-01 2.22% 1.70E-01 2.95% 1.70E-01 2.96%

6.30E-02 0.95% 4.51E-02 1.22% 7.78E-02 1.15% 6.78E-02 1.18% 7.55E-02 1.31%

2.10E-02 0.32% 1.63E-02 0.44% 2.80E-02 0.41% 2.90E-02 0.50% 2.74E-02 0.48%

3.98E-02 0.60% 3.14E-02 0.85% 6.16E-02 0.91% 4.42E-02 0.77% 4.54E-02 0.79%

9.08E-03 0.14% 7.44E-03 0.20% 1.11E-02 0.16% 1.13E-02 0.20% 1.03E-02 0.18%

1.51E-02 0.23% 1.44E-02 0.39% 1.96E-02 0.29% 1.95E-02 0.34% 1.90E-02 0.33%

8.00E-04 D 0.01% 4.95E-04 D 0.01% 1.10E-03 0.02% 9.50E-04 0.02% 1.00E-03 0.02%

6.60E+00 3.69E+00 6.76E+00 5.76E+00 5.75E+00

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163/164, so this value was not included in total TEQ sum.

3) PCB congener 169 was not detected in any samples, so was not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.

Station

Chemical

PCB066

PCB077

PCB105

PCB110

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB170

PCB180

PCB194

Total TEQ
Table B2-1. Continued

F5-1 Percent of F5-2 Percent of F5-3 Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

6.40E-01 27.06% 6.40E-01 24.82% 7.20E-01 25.23%

3.09E-01 13.06% 3.09E-01 11.98% 3.15E-01 11.04%

7.00E-01 29.60% 8.00E-01 31.02% 9.00E-01 31.54%

3.65E-02 1.54% 4.10E-02 1.59% 4.70E-02 1.65%

4.20E-01 17.76% 4.60E-01 17.84% 5.20E-01 18.22%

1.18E-01 5.00% 1.57E-01 6.07% 1.66E-01 5.82%

6.40E-02 2.71% 8.10E-02 3.14% 9.00E-02 3.15%

3.37E-02 1.42% 3.56E-02 1.38% 3.72E-02 1.30%

1.03E-02 0.44% 1.26E-02 0.49% 1.33E-02 0.46%

2.10E-02 0.89% 2.80E-02 1.09% 2.66E-02 0.93%

4.08E-03 0.17% 5.02E-03 0.19% 5.66E-03 0.20%

7.96E-03 0.34% 9.60E-03 0.37% 1.19E-02 0.42%

3.60E-04 0.02% 3.75E-04 0.01% 6.00E-04 0.02%

2.37E+00 2.58E+00 2.85E+00

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163/164, so this value was not included in total TEQ sum.

3) PCB congener 169 was not detected in any samples, so was not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.

Station

Chemical

PCB066

PCB077

PCB105

PCB110

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB170

PCB180

PCB194

Total TEQ
Table B2-2. Bird TEQs using 1997 ERA sediment data

Station S1-1 Percent of S1-2 Percent of S1-3 Percent of

Chemical Bird TEFs TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

PCB066 0.002 3.00E-04 20.05% 7.20E-04 7.92% 1.20E-04 R 8.82%

PCB077 0.03 1.19E-04 7.98% 1.09E-03 11.98% 2.61E-04 19.20%

PCB105 0.005 6.50E-04 43.43% 2.75E-03 30.24% 5.00E-04 36.73%

PCB110 0.00005 2.00E-05 1.34% 1.05E-04 1.15% 1.90E-05 1.40%

PCB118 0.001 2.40E-04 16.04% 1.30E-03 14.30% 2.40E-04 17.63%

PCB126 0.3 7.65E-05 U 5.11% 2.14E-03 L 23.56% 7.50E-05 U 5.51%

PCB128 0.001 5.00E-05 U 3.34% 5.40E-04 5.94% 6.00E-05 R 4.41%

PCB156 0.001 1.15E-05 L 0.77% 1.34E-04 1.47% 2.60E-05 1.91%

PCB157 0.002 4.74E-06 L 0.32% 5.78E-05 0.64% 1.19E-05 L 0.87%

PCB167 0.002 1.20E-05 L 0.80% 1.18E-04 1.30% 2.18E-05 L 1.60%

PCB170 0.0002 3.60E-06 L 0.24% 5.76E-05 0.63% 9.66E-06 0.71%

PCB180 0.0002 6.32E-06 0.42% 7.34E-05 0.81% 1.41E-05 1.04%

PCB194 0.00005 2.50E-06 U 0.17% 6.00E-06 R 0.07% 2.50E-06 U 0.18%

Total TEQ 1.50E-03 9.09E-03 1.36E-03

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was 

 detected in another sample. This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163/164, so this value was not included in total TEQ sum.

3) PCB congener 169 was not detected in any samples, so was not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.
Table B2-2. Continued

Station

Chemical

PCB066

PCB077

PCB105

PCB110

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB170

PCB180

PCB194

Total TEQ

S2-1 Percent of S2-2 Percent of S2-3 Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

2.20E-01 30.41% 1.44E+00 20.95% 2.00E-01 22.98%

2.05E-01 28.37% 2.05E+00 29.77% 2.74E-01 31.43%

1.60E-01 22.12% 1.85E+00 26.92% 1.90E-01 21.83%

9.00E-03 1.24% 6.50E-02 0.95% 1.20E-02 1.38%

7.60E-02 10.51% 7.10E-01 10.33% 9.90E-02 11.37%

3.45E-02 4.77% 5.28E-01 7.68% 6.09E-02 7.00%

9.30E-03 1.29% 1.30E-01 1.89% 1.70E-02 1.95%

3.36E-03 0.46% 3.92E-02 0.57% 6.42E-03 0.74%

1.33E-03 0.18% 1.58E-02 0.23% 2.62E-03 0.30%

2.54E-03 0.35% 2.60E-02 0.38% 4.76E-03 0.55%

8.20E-04 0.11% 8.60E-03 0.13% 1.68E-03 0.19%

1.26E-03 0.17% 1.24E-02 0.18% 2.38E-03 0.27%

8.50E-05 0.01% 1.00E-03 0.01% 1.20E-04 0.01%

7.23E-01 6.87E+00 8.70E-01

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was 

 detected in another sample. This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163/164, so this value was not included in total TEQ sum.

3) PCB congener 169 was not detected in any samples, so was not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.
Table B2-2. Continued

Station

Chemical

PCB066

PCB077

PCB105

PCB110

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB170

PCB180

PCB194

Total TEQ

S3-1 Percent of S3-2 Percent of S3-3 Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

1.08E-01 18.40% 2.80E-01 21.88% 1.50E-01 20.03%

2.26E-01 38.50% 3.39E-01 26.49% 2.50E-01 33.37%

1.10E-01 18.75% 3.35E-01 26.18% 1.65E-01 22.04%

9.00E-03 1.53% 3.30E-02 2.58% 8.50E-03 1.14%

5.60E-02 9.54% 1.20E-01 9.38% 7.30E-02 9.75%

5.31E-02 9.05% 1.02E-01 7.97% 6.87E-02 9.17%

9.80E-03 1.67% 2.50E-02 R 1.95% 1.20E-02 1.60%

5.04E-03 0.86% 1.38E-02 1.08% 8.18E-03 1.09%

2.22E-03 0.38% 4.96E-03 0.39% 3.62E-03 0.48%

3.64E-03 0.62% 9.36E-03 0.73% 5.40E-03 0.72%

1.54E-03 0.26% 6.26E-03 0.49% 1.73E-03 0.23%

2.44E-03 0.42% 1.02E-02 0.80% 2.64E-03 0.35%

1.30E-04 0.02% 9.00E-04 U 0.07% 1.20E-04 U 0.02%

5.87E-01 1.28E+00 7.49E-01

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was 

 detected in another sample. This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163/164, so this value was not included in total TEQ sum.

3) PCB congener 169 was not detected in any samples, so was not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.
Table B2-2. Continued

Station

Chemical

PCB066

PCB077

PCB105

PCB110

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB170

PCB180

PCB194

Total TEQ

S5-1 Percent of S5-2 Percent of S5-3 Percent of S5-4 Percent of S5-5 Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

1.80E-01 18.85% 3.40E-02 18.80% 1.74E-01 21.21% 7.80E-02 16.82% 3.40E-02 21.09%

3.27E-01 34.25% 6.30E-02 34.84% 2.59E-01 31.52% 1.90E-01 40.89% 5.46E-02 D 33.87%

2.30E-01 24.09% 4.40E-02 24.33% 2.00E-01 24.38% 8.50E-02 18.33% 3.60E-02 22.33%

9.00E-03 0.94% 1.70E-03 0.94% 6.50E-03 0.79% 3.10E-03 0.67% 1.65E-03 1.02%

8.10E-02 8.48% 1.50E-02 8.30% 7.30E-02 8.90% 3.40E-02 7.33% 1.50E-02 9.30%

8.97E-02 9.40% 1.86E-02 10.27% 8.28E-02 10.09% 5.82E-02 12.55% 1.44E-02 8.91%

1.90E-02 1.99% 1.05E-03 U 0.58% 7.60E-03 0.93% 4.20E-03 0.91% 2.50E-03 1.55%

7.53E-03 0.79% 1.43E-03 0.79% 6.95E-03 0.85% 4.71E-03 1.02% 1.12E-03 0.69%

3.08E-03 0.32% 5.98E-04 0.33% 3.10E-03 0.38% 1.91E-03 0.41% 4.72E-04 0.29%

4.68E-03 0.49% 9.20E-04 0.51% 4.58E-03 0.56% 2.80E-03 0.60% 8.40E-04 0.52%

1.33E-03 0.14% 2.04E-04 0.11% 1.30E-03 0.16% 8.20E-04 0.18% 2.56E-04 0.16%

2.12E-03 0.22% 3.26E-04 0.18% 1.93E-03 0.24% 1.28E-03 0.28% 3.76E-04 0.23%

2.75E-04 U 0.03% 1.70E-05 U 0.01% 9.00E-05 0.01% 5.50E-05 0.01% 2.15E-05 0.01%

9.55E-01 1.81E-01 8.20E-01 4.64E-01 1.61E-01

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was 

 detected in another sample. This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163/164, so this value was not included in total TEQ sum.

3) PCB congener 169 was not detected in any samples, so was not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.
Table B2-3. Bird TEQs using young-of-year fish tissue samples collected for the WDNR food chain study

Station 603388 603388 603388

Sample 9413 9414 9415

Species longnose dace longnose dace longnose dace 

Location control control control 

Chemical Bird TEFs TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

PCB077 0.03 0.015 U 6.01% na na

PCB105 0.005 0.0495 19.85% na na

PCB118 0.001 0.025 10.02% 0.0068 na 0.0033 na

PCB126 0.3 0.15 U 60.15% na na

PCB128 0.001 0.0035 1.40% 0.0016 na 0.0007 U na

PCB156 0.001 0.0024 0.96% na na

PCB157 0.002 0.001 U 0.40% na na

PCB167 0.002 0.0018 U 0.72% 0.0018 U na 0.0018 U na

PCB180 0.0002 0.00116 0.47% 0.00058 na 0.00022 U na

PCB194 0.00005 0.000025 U 0.01% 0.000025 U na 0.000025 U na

Total TEQ 0.249385 na na

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in Percent of Total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.

5) PCB congeners 77, 105, 126, 156, and 157 were only analyzed in some samples.

6) na - not applicable when all TEF congeners are not measured.
Table B2-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603389 603389 603389 603390

9413 9414 9415 9413

longnose dace longnose dace longnose dace longnose dace 

rochester park rochester park rochester park esselingen park 

TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

na na 0.48 16.49% 0.6 19.46%

na na 1.2 41.23% 1.3 42.16%

0.3 na 0.32 na 0.74 25.42% 0.76 24.65%

na na 0.3 U*I 10.31% 0.15 U 4.86%

0.034 na 0.036 na 0.071 2.44% 0.087 2.82%

na na 0.044 1.51% 0.054 1.75%

na na 0.022 0.76% 0.052 1.69%

0.022 na 0.024 na 0.038 1.31% 0.056 1.82%

0.0106 na 0.0114 na 0.0154 0.53% 0.024 0.78%

0.000345 na 0.00035 na 0.000445 0.02% 0.0007 0.02%

na na 2.910845 3.0837

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in Percent of Total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.

5) PCB congeners 77, 105, 126, 156, and 157 were only analyzed in some samples.

6) na - not applicable when all TEF congeners are not measured.
Table B2-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603390 603390 603391 603391

9414 9415 9413 9414

longnose dace longnose dace longnose dace longnose dace 

esselingen park esselingen park between kohler dams between kohler dams

TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

na na na na

na na na na

0.7 na 0.46 na 0.46 na 0.56 na

na na na na

0.078 na 0.053 na 0.054 na 0.063 na

na na na na

na na na na

0.044 na 0.028 na 0.032 na 0.038 na

0.02 na 0.0152 na 0.0164 na 0.019 na

0.00065 na 0.000425 na 0.00055 na 0.0006 na

na na na na

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in Percent of Total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.

5) PCB congeners 77, 105, 126, 156, and 157 were only analyzed in some samples.

6) na - not applicable when all TEF congeners are not measured.
Table B2-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603391 603388 603388 603388

9415 9404 9405 9406

longnose dace smallmouth bass smallmouth bass smallmouth bass 

between kohler dams control control control 

TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

0.51 22.41% na na 0.015 U 8.15%

0.85 37.36% na na 0.007 3.80%

0.56 24.61% 0.0039 na 0.0044 na 0.0076 4.13%

0.15 U 6.59% na na 0.15 U 81.48%

0.065 2.86% 0.0007 U na 0.0007 U na 0.0007 U 0.38%

0.038 1.67% na na 0.0005 U 0.27%

0.038 1.67% na na 0.001 U 0.54%

0.044 1.93% 0.0018 U na 0.0018 U na 0.0018 U 0.98%

0.0198 0.87% 0.00022 U na 0.00022 U na 0.00048 0.26%

0.00065 0.03% 0.000025 U na 0.000025 U na 0.000025 U 0.01%

2.27545 na na 0.184105

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in Percent of Total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.

5) PCB congeners 77, 105, 126, 156, and 157 were only analyzed in some samples.

6) na - not applicable when all TEF congeners are not measured.
Table B2-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603389 603389 603389 603390

9404 9405 9406 9404

smallmouth bass smallmouth bass smallmouth bass smallmouth bass 

rochester park rochester park rochester park esselingen park 

TEQ Q

Percent 

of Total 

TEQ TEQ Q

Percent 

of Total 

TEQ TEQ Q

Percent 

of Total 

TEQ TEQ Q

Percent 

of Total 

TEQ

na na 0.87 21.23% na

na na 1.7 41.49% na

0.92 na 0.72 na 0.92 22.45% 0.25 na

na na 0.36 U*I 8.79% na

0.09 na 0.11 na 0.093 2.27% 0.032 na

na na 0.054 1.32% na

na na 0.032 0.78% na

0.064 na 0.076 na 0.048 1.17% 0.0186 na

0.02 na 0.024 na 0.02 0.49% 0.0096 na

0.0006 na 0.0008 na 0.0007 0.02% 0.00029 na

na na 4.0977 na

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in Percent of Total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.

5) PCB congeners 77, 105, 126, 156, and 157 were only analyzed in some samples.

6) na - not applicable when all TEF congeners are not measured.
Table B2-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603390 603390 603391 603391

9405 9406 9404 9405

smallmouth bass smallmouth bass smallmouth bass smallmouth bass 

esselingen park esselingen park between kohler dams between kohler dams

TEQ Q

Percent 

of Total 

TEQ TEQ Q

Percent 

of Total 

TEQ TEQ Q

Percent 

of Total 

TEQ TEQ Q

Percent 

of Total 

TEQ

na 0.42 20.75% na na

na 0.85 42.00% na na

0.36 na 0.48 23.72% 0.83 na 0.72 na

na 0.15 U 7.41% na na

0.045 na 0.049 2.42% 0.1 na 0.088 na

na 0.023 1.14% na na

na 0.0136 0.67% na na

0.026 na 0.026 1.28% 0.054 na 0.048 na

0.0136 na 0.012 0.59% 0.022 na 0.02 na

0.000415 na 0.000325 0.02% 0.0007 na 0.00075 na

na 2.023925 na na

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in Percent of Total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.

5) PCB congeners 77, 105, 126, 156, and 157 were only analyzed in some samples.

6) na - not applicable when all TEF congeners are not measured.
Table B2-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603391 603388 603388 603388

9406 9410 9411 9412

smallmouth bass white sucker white sucker white sucker 

between kohler dams control control control 

TEQ Q

Percent 

of Total 

TEQ TEQ Q

Percent 

of Total 

TEQ TEQ Q

Percent 

of Total 

TEQ TEQ Q

Percent 

of Total 

TEQ

1.23 21.70% na na 0.015 U 7.61%

2.15 37.93% na na 0.016 8.12%

1.2 21.17% 0.0013 na 0.0016 na 0.011 5.58%

0.6 10.59% na na 0.15 U 76.09%

0.16 2.82% 0.0007 U na 0.0007 U na 0.0016 0.81%

0.099 1.75% na na 0.0005 U 0.25%

0.072 1.27% na na 0.001 U 0.51%

0.116 2.05% 0.0018 U na 0.0018 U na 0.0018 U 0.91%

0.04 0.71% 0.00022 U na 0.00022 U na 0.00022 U 0.11%

0.00115 0.02% 0.000025 U na 0.000025 U na 0.000025 U 0.01%

5.66815 na na 0.197145

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in Percent of Total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.

5) PCB congeners 77, 105, 126, 156, and 157 were only analyzed in some samples.

6) na - not applicable when all TEF congeners are not measured.
Table B2-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603389 603389 603389 603390

9410 9411 9412 9410

white sucker white sucker white sucker white sucker 

rochester park rochester park rochester park esselingen park 

TEQ Q

Percent 

of Total 

TEQ TEQ Q

Percent 

of Total 

TEQ TEQ Q

Percent 

of Total 

TEQ TEQ Q

Percent 

of Total 

TEQ

na na 0.33 19.64% na

na na 0.65 38.69% na

0.37 na 0.4 na 0.31 18.45% 0.14 na

na na 0.285 U*I 16.96% na

0.05 na 0.05 na 0.041 2.44% 0.022 na

na na 0.022 1.31% na

na na 0.012 0.71% na

0.026 na 0.026 na 0.02 1.19% 0.0108 na

0.013 na 0.0126 na 0.0096 0.57% 0.0042 na

0.0005 na 0.00044 na 0.00034 0.02% 0.00016 na

na na 1.67994 na

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in Percent of Total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.

5) PCB congeners 77, 105, 126, 156, and 157 were only analyzed in some samples.

6) na - not applicable when all TEF congeners are not measured.
Table B2-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603390 603390 603391 603391

9411 9412 9410 9411

white sucker white sucker white sucker white sucker 

esselingen park esselingen park between kohler dams between kohler dams

TEQ Q

Percent 

of Total 

TEQ TEQ Q

Percent 

of Total 

TEQ TEQ Q

Percent 

of Total 

TEQ TEQ Q

Percent 

of Total 

TEQ

na 0.192 21.00% na na

na 0.305 33.36% na na

0.14 na 0.2 21.87% 0.25 na 0.45 na

na 0.15 U 16.40% na na

0.02 na 0.025 2.73% 0.036 na 0.059 na

na 0.013 1.42% na na

na 0.011 1.20% na na

0.0086 na 0.0124 1.36% 0.015 na 0.024 na

0.0044 na 0.0058 0.63% 0.0078 na 0.0124 na

0.00015 na 0.000185 0.02% 0.000235 na 0.000415 na

na 0.914385 na na

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in Percent of Total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.

5) PCB congeners 77, 105, 126, 156, and 157 were only analyzed in some samples.

6) na - not applicable when all TEF congeners are not measured.
Table B2-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603391

9412

white sucker 

between kohler dams

TEQ Q

Percent 

of Total 

TEQ

0.234 20.37%

0.415 36.12%

0.26 22.63%

0.15 U 13.06%

0.031 2.70%

0.019 1.65%

0.0144 1.25%

0.017 1.48%

0.0082 0.71%

0.00027 0.02%

1.14887

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in Percent of Total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.

5) PCB congeners 77, 105, 126, 156, and 157 were only analyzed in some samples.

6) na - not applicable when all TEF congeners are not measured.
Table B2-4. Bird TEQs using crayfish samples collected for the WDNR food chain study

Station 603384 603384 603384

Sample 9409 9410 9411

Species crayfish crayfish crayfish 

Location control control control 

Chemical Bird TEFs TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

PCB077 0.03 0.015 U 8.71% 0.015 U 8.71% na

PCB105 0.005 0.0025 U 1.45% 0.0025 U 1.45% na

PCB118 0.001 0.0004 U 0.23% 0.0004 U 0.23% 0.0015 na

PCB126 0.3 0.15 U 87.14% 0.15 U 87.14% na

PCB128 0.001 0.0007 U 0.41% 0.0007 U 0.41% 0.0007 U na

PCB156 0.001 0.0005 U 0.29% 0.0005 U 0.29% na

PCB157 0.002 0.001 U 0.58% 0.001 U 0.58% na

PCB167 0.002 0.0018 U 1.05% 0.0018 U 1.05% 0.0018 U na

PCB180 0.0002 0.00022 U 0.13% 0.00022 U 0.13% 0.00022 U na

PCB194 0.00005 0.000025 U 0.01% 0.000025 U 0.01% 0.000025 U na

Total TEQ 0.172145 0.172145 na

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.

5) PCB congeners 77, 105, 126, 156, and 157 were only analyzed in some samples.

6) na - not applicable when all TEF congeners are not measured.
Table B2-4. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603385 603385 603385

9409 9410 9411

crayfish crayfish crayfish 

rochester park rochester park rochester park 

TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

0.156 26.90% 0.132 26.43% na

0.145 25.00% 0.11 22.02% na

0.098 16.90% 0.083 16.62% 0.079 na

0.15 U 25.86% 0.15 U 30.03% na

0.01 1.72% 0.0079 1.58% 0.01 na

0.0063 1.09% 0.0051 1.02% na

0.0042 0.72% 0.0034 0.68% na

0.007 1.21% 0.0054 1.08% 0.0066 na

0.0034 0.59% 0.0026 0.52% 0.0028 na

0.00012 0.02% 0.00007 0.01% 0.00008 na

0.58002 0.49947 na

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.

5) PCB congeners 77, 105, 126, 156, and 157 were only analyzed in some samples.

6) na - not applicable when all TEF congeners are not measured.
Table B2-4. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603386 603386 603386

9409 9410 9411

crayfish crayfish crayfish 

between kohler dams between kohler dams between kohler dams

TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

0.075 *Q 14.49% 0.057 *Q 13.01% na

0.13 25.12% 0.105 23.97% na

0.13 25.12% 0.095 21.69% 0.088 na

0.15 U 28.98% 0.15 U 34.25% na

0.012 2.32% 0.011 2.51% 0.012 na

0.0058 1.12% 0.0063 1.44% na

0.0028 0.54% 0.0028 0.64% na

0.0078 1.51% 0.0072 1.64% 0.0068 na

0.004 0.77% 0.0036 0.82% 0.0032 na

0.00012 0.02% 0.0001 0.02% 0.00009 na

0.51752 0.438 na

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.

5) PCB congeners 77, 105, 126, 156, and 157 were only analyzed in some samples.

6) na - not applicable when all TEF congeners are not measured.
Table B2-4. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603387 603387 603387

9409 9410 9411

crayfish crayfish crayfish 

esselingen park esselingen park esselingen park 

TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

0.069 16.37% 0.084 17.96% na

0.095 22.54% 0.115 24.59% na

0.08 18.98% 0.087 18.60% 0.083 na

0.15 U 35.59% 0.15 U 32.08% na

0.0094 2.23% 0.011 2.35% 0.012 na

0.0039 0.93% 0.0047 1.01% na

0.0042 1.00% 0.0046 0.98% na

0.0066 1.57% 0.0076 1.63% 0.0072 na

0.0032 0.76% 0.0036 0.77% 0.0036 na

0.000115 0.03% 0.000125 0.03% 0.000095 na

0.421415 0.467625 na

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.

5) PCB congeners 77, 105, 126, 156, and 157 were only analyzed in some samples.

6) na - not applicable when all TEF congeners are not measured.
Table B2-5. Bird TEQs using sediment samples collected for the WDNR food chain study

Station 603363 603364 603365

Sample 6A 6B 6C 

Location camp marina Percent of camp marina Percent of camp marina Percent of

Chemical Bird TEFs TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

PCB077 0.03 0.174 40.09% 0.168 48.31% 0.24 45.54%

PCB105 0.005 0.12 27.65% 0.095 27.32% 0.15 28.46%

PCB118 0.001 0.045 10.37% 0.039 11.22% 0.055 10.44%

PCB126 0.3 0.078 17.97% 0.03 U 8.63% 0.0615 U*I 11.67%

PCB128 0.001 0.0063 1.45% 0.0056 1.61% 0.008 1.52%

PCB156 0.001 0.0041 0.94% 0.0032 0.92% 0.0046 0.87%

PCB157 0.002 0.0022 0.51% 0.00186 0.53% 0.0028 0.53%

PCB167 0.002 0.0028 0.65% 0.0024 0.69% 0.0034 0.65%

PCB180 0.0002 0.0015 0.35% 0.0024 0.69% 0.00164 0.31%

PCB194 0.00005 0.000075 0.02% 0.00026 0.07% 0.000075 0.01%

Total TEQ 0.433975 0.34772 0.527015

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.
Table B2-5. Continued

Station

Sample

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603366 603367 603368

6D 6E 5A 

camp marina Percent of camp marina Percent of kiwanis park Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.237 47.38% 0.12 45.97% 0.219 43.76%

0.145 28.99% 0.075 28.73% 0.17 33.97%

0.053 10.60% 0.026 9.96% 0.059 11.79%

0.045 U*I 9.00% 0.03 U 11.49% 0.03 U 5.99%

0.008 1.60% 0.0037 1.42% 0.0087 1.74%

0.0045 0.90% 0.0023 0.88% 0.0053 1.06%

0.0026 0.52% 0.00138 0.53% 0.0028 0.56%

0.0034 0.68% 0.0017 0.65% 0.0038 0.76%

0.00162 0.32% 0.00092 0.35% 0.0018 0.36%

0.00007 0.01% 4.75E-05 0.02% 0.00008 0.02%

0.50019 0.261048 0.50048

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.
Table B2-5. Continued

Station

Sample

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603369 603370 603371

5B 5C 5D 

kiwanis park Percent of kiwanis park Percent of kiwanis park Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.225 46.26% 0.138 43.69% 0.186 43.63%

0.15 30.84% 0.1 31.66% 0.145 34.02%

0.055 11.31% 0.035 11.08% 0.048 11.26%

0.0345 U*I 7.09% 0.03 U 9.50% 0.03 U 7.04%

0.0086 1.77% 0.0051 1.61% 0.0068 1.60%

0.0049 1.01% 0.003 0.95% 0.0039 0.91%

0.003 0.62% 0.00184 0.58% 0.0024 0.56%

0.0036 0.74% 0.002 0.63% 0.0028 0.66%

0.00174 0.36% 0.00092 0.29% 0.00132 0.31%

0.00007 0.01% 3.45E-05 0.01% 0.00005 0.01%

0.48641 0.315895 0.42627

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.
Table B2-5. Continued

Station

Sample

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603372 603373 603374

3A 3B 3C 

between kohler dams Percent of between kohler dams Percent of between kohler dams Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.3 40.59% 0.258 44.56% 0.156 45.70%

0.235 31.80% 0.17 29.36% 0.095 27.83%

0.089 12.04% 0.068 11.74% 0.04 11.72%

0.081 10.96% 0.054 U*I 9.33% 0.0345 U*I 10.11%

0.013 1.76% 0.011 1.90% 0.0059 1.73%

0.0079 1.07% 0.0067 1.16% 0.0035 1.03%

0.0046 0.62% 0.004 0.69% 0.0022 0.64%

0.0056 0.76% 0.0048 0.83% 0.0026 0.76%

0.0028 0.38% 0.0024 0.41% 0.0016 0.47%

0.00014 0.02% 0.00011 0.02% 0.00008 0.02%

0.73904 0.57901 0.34138

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.
Table B2-5. Continued

Station

Sample

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603375 603376 603377

3D 3E 2A 

between kohler dams Percent of between kohler dams Percent of rochester park Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.27 46.87% 0.294 44.89% 0.21 44.14%

0.165 28.64% 0.185 28.25% 0.145 30.48%

0.079 13.71% 0.088 13.44% 0.059 12.40%

0.03 U 5.21% 0.0525 U*I 8.02% 0.039 U*I 8.20%

0.012 2.08% 0.014 2.14% 0.0087 1.83%

0.0071 1.23% 0.0079 1.21% 0.005 1.05%

0.0044 0.76% 0.0046 0.70% 0.0032 0.67%

0.0054 0.94% 0.0056 0.86% 0.0038 0.80%

0.003 0.52% 0.0032 0.49% 0.002 0.42%

0.000165 0.03% 0.000145 0.02% 0.000095 0.02%

0.576065 0.654945 0.475795

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.
Table B2-5. Continued

Station

Sample

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603378 603379 603380

2B 2C 2D 

rochester park Percent of rochester park Percent of rochester park Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.45 43.54% 0.219 47.82% 0.81 42.68%

0.33 31.93% 0.125 27.29% 0.5 26.34%

0.12 11.61% 0.06 13.10% 0.29 15.28%

0.09 U*I 8.71% 0.033 U*I 7.21% 0.18 U*I 9.48%

0.017 1.64% 0.0084 1.83% 0.047 2.48%

0.01 0.97% 0.0045 0.98% 0.026 1.37%

0.006 0.58% 0.0026 0.57% 0.016 0.84%

0.007 0.68% 0.0036 0.79% 0.0196 1.03%

0.0034 0.33% 0.00182 0.40% 0.009 0.47%

0.00015 0.01% 0.00008 0.02% 0.00032 0.02%

1.03355 0.458 1.89792

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.
Table B2-5. Continued

Station

Sample

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603381 603392 603393

2E 1A 1B 

rochester park Percent of control Percent of control Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.9 40.83% 0.003 U 7.93% 0.003 U 8.38%

0.8 36.30% 0.0005 U 1.32% 0.00115 3.21%

0.29 13.16% 0.000225 U 0.59% 0.00054 1.51%

0.0975 U*I 4.42% 0.033 U 87.25% 0.03 U 83.83%

0.047 2.13% 0.00025 U 0.66% 0.00025 U 0.70%

0.027 1.22% 0.0001 U 0.26% 0.0001 U 0.28%

0.0162 0.73% 0.0002 U 0.53% 0.0002 U 0.56%

0.0186 0.84% 0.0005 U 1.32% 0.0005 U 1.40%

0.0076 0.34% 0.000035 U 0.09% 0.000035 U 0.10%

0.00022 0.01% 1.25E-05 U 0.03% 1.25E-05 U 0.03%

2.20412 0.037823 0.035788

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.
Table B2-5. Continued

Station

Sample

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB128

PCB156

PCB157

PCB167

PCB180

PCB194

Total TEQ

603394 603395 603396

1C 1D 1E 

control Percent of control Percent of control Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.003 U 8.56% 0.003 U 8.19% 0.003 U 8.27%

0.0005 U 1.43% 0.0017 4.64% 0.0014 3.86%

0.00046 1.31% 0.00082 2.24% 0.00076 2.10%

0.03 U 85.57% 0.03 U 81.93% 0.03 U 82.74%

0.00025 U 0.71% 0.00025 U 0.68% 0.00025 U 0.69%

0.0001 U 0.29% 0.0001 U 0.27% 0.0001 U 0.28%

0.0002 U 0.57% 0.0002 U 0.55% 0.0002 U 0.55%

0.0005 U 1.43% 0.0005 U 1.37% 0.0005 U 1.38%

0.000035 U 0.10% 0.000035 U 0.10% 0.000035 U 0.10%

1.25E-05 U 0.04% 1.25E-05 U 0.03% 1.25E-05 U 0.03%

0.035058 0.036618 0.036258

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 138 was measured as 138/163 and PCB congener 170 as 170/190, so these were not included in total TEQ sum.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 2, 12, 35, 37, 66, 78, 79, 80, 81, 110, 122, 127, and 139 were not analyzed in any samples.
APPENDIX B-3

Toxic Equivalent Concentrations for Mammals
LIST OF TABLES

Table B3-1. TEQs for mammals by chemical and station using 1997 ERA tissue

data

Table B3-2. TEQs for mammals by chemical and station using 1997 ERA

sediment data

Table B3-3. Mammal TEQs using fish tissue samples collected for the WDNR

food chain study

Table B3-4. Mammal TEQs using sediment data collected for the WDNR food

chain study
APPENDIX B-3

DEFINITION OF TERMS

Definition of qualifiers

> - sample over linear range of method

D - diluted sample

L - labeled compound

U - undetected 

U*I - significant interference present; value represents operator's best judgement of reporting limit

1997 ERA sample identification 

S1-1

sediment sample river segment sample number

F1-1

fish tissue sample river segment sample number
Table B3-1. TEQs for mammals by chemical and station using 1997 ERA tissue data

Station F1-1 Percent of F1-2 Percent of F1-3 Percent of F2-1 Percent of

Chemical Mammal TEFs TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

PCB077 0.0001 1.07E-05 0.34% 1.15E-05 0.33% 1.00E-05 0.26% 3.94E-03 D 1.61%

PCB105 0.0001 2.40E-04 7.64% 2.50E-04 7.28% 2.30E-04 6.03% 3.00E-02 >D 12.25%

PCB114 0.0005 9.30E-05 L 2.96% 8.15E-05 L 2.37% 1.39E-04 3.63% 1.44E-02 5.86%

PCB118 0.0001 7.80E-04 24.84% 7.90E-04 23.00% 7.50E-04 19.65% 5.70E-02 >D 23.28%

PCB123 0.0001 8.88E-06 0.28% 7.22E-06 0.21% 1.52E-05 0.40% 1.17E-03 0.48%

PCB126 0.1 1.44E-03 45.86% 1.69E-03 49.20% 2.12E-03 55.55% 1.07E-01 43.70%

PCB156 0.0005 4.79E-04 15.25% 5.05E-04 14.70% 4.37E-04 11.45% 2.57E-02 10.50%

PCB157 0.0005 8.35E-05 2.66% 9.30E-05 2.71% 1.09E-04 2.84% 5.45E-03 2.23%

PCB189 0.0001 5.24E-06 0.17% 6.58E-06 0.19% 6.87E-06 0.18% 2.16E-04 0.09%

Total TEQ 3.14E-03 3.43E-03 3.82E-03 2.45E-01

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congener 169 was not detected in any samples, so was not included in the TEQ sum.
Table B3-1. Continued

Station

Chemical

PCB077

PCB105

PCB114

PCB118

PCB123

PCB126

PCB156

PCB157

PCB189

Total TEQ

F2-2 Percent of F2-3 Percent of F3-1 Percent of F3-2 Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

5.30E-03 D 1.59% 2.54E-03 1.34% 3.52E-03 D 0.94% 0.00244 0.76%

3.30E-02 D 9.92% 2.10E-02 >D 11.05% 3.90E-02 10.36% 0.035 10.84%

1.64E-02 4.91% 1.20E-02 6.31% 1.73E-02 4.58% 0.01665 5.16%

8.90E-02 >D 26.75% 4.80E-02 >D 25.26% 1.10E-01 29.23% 0.097 30.04%

1.10E-03 0.33% 1.09E-03 0.57% 1.32E-03 0.35% 0.00139 0.43%

1.51E-01 45.38% 7.86E-02 41.36% 1.59E-01 42.25% 0.129 39.95%

3.15E-02 9.47% 2.26E-02 11.87% 3.89E-02 10.34% 0.0339 10.50%

5.25E-03 1.58% 4.08E-03 2.15% 7.00E-03 1.86% 0.00725 2.25%

2.35E-04 0.07% 1.80E-04 0.09% 2.99E-04 0.08% 0.000284 0.09%

3.33E-01 1.90E-01 3.76E-01 3.23E-01

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congener 169 was not detected in any samples, so was not included in the TEQ sum.
Table B3-1. Continued

Station

Chemical

PCB077

PCB105

PCB114

PCB118

PCB123

PCB126

PCB156

PCB157

PCB189

Total TEQ

F3-3 Percent of F5-1 Percent of F5-2 Percent of F5-3 Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.00266 D 0.79% 0.00103 0.83% 0.00103 0.71% 0.00105 0.66%

0.033 9.76% 0.014 11.33% 0.016 10.97% 0.018 11.32%

0.01445 4.27% 0.00695 5.63% 0.0087 5.97% 0.0099 6.22%

0.097 28.68% 0.042 33.99% 0.046 31.54% 0.052 32.69%

0.00115 0.34% 0.000617 0.50% 0.000808 0.55% 0.000651 0.41%

0.145 42.88% 0.0394 31.89% 0.0522 35.79% 0.0554 34.83%

0.03775 11.16% 0.01685 13.64% 0.0178 12.20% 0.0186 11.69%

0.00685 2.03% 0.002585 2.09% 0.003145 2.16% 0.003315 2.08%

0.000318 0.09% 0.000117 0.09% 0.000167 0.11% 0.000153 0.10%

3.38E-01 1.24E-01 1.46E-01 1.59E-01

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congener 169 was not detected in any samples, so was not included in the TEQ sum.
Table B3-2. TEQs for mammals by chemical and station using 1997 ERA sediment data

Station S1-1 Percent of S1-2 Percent of S1-3 Percent of S2-1 Percent of

Chemical Mammal TEFs TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

PCB077 0.0001 3.98E-07 0.56% 3.63E-06 0.36% 8.71E-07 1.11% 6.84E-04 2.60%

PCB105 0.0001 1.30E-05 18.26% 5.50E-05 5.52% 1.00E-05 12.71% 3.20E-03 12.16%

PCB114 0.0005 1.22E-06 L 1.71% 1.04E-05 L 1.04% 2.31E-06 L 2.94% 1.20E-03 4.56%

PCB118 0.0001 2.40E-05 33.70% 1.30E-04 13.06% 2.40E-05 30.50% 7.60E-03 28.89%

PCB123 0.0001 1.09E-07 L 0.15% 1.10E-06 0.11% 4.96E-07 L 0.63% 9.66E-05 0.37%

PCB126 0.1 2.55E-05 U 35.81% 7.14E-04 L 71.71% 2.50E-05 U 31.77% 1.15E-02 43.71%

PCB156 0.0005 5.75E-06 L 8.07% 6.70E-05 6.73% 1.30E-05 16.52% 1.68E-03 6.39%

PCB157 0.0005 1.19E-06 L 1.66% 1.45E-05 1.45% 2.97E-06 L 3.77% 3.32E-04 1.26%

PCB189 0.0001 5.00E-08 U 0.07% 5.00E-08 U 0.01% 5.00E-08 U 0.06% 1.82E-05 0.07%

Total TEQ 7.12E-05 9.96E-04 7.87E-05 2.63E-02

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congener 169 was not detected in any samples, so was not included in the TEQ sum.
Table B3-2. Continued

Station

Chemical

PCB077

PCB105

PCB114

PCB118

PCB123

PCB126

PCB156

PCB157

PCB189

Total TEQ

S2-2 Percent of S2-3 Percent of S3-1 Percent of S3-2 Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

6.82E-03 2.08% 9.12E-04 2.25% 7.53E-04 2.43% 1.13E-03 1.73%

3.70E-02 11.31% 3.80E-03 9.37% 2.20E-03 7.11% 6.70E-03 10.26%

1.15E-02 3.52% 1.59E-03 3.91% 1.47E-03 4.73% 2.94E-03 4.49%

7.10E-02 21.70% 9.90E-03 24.42% 5.60E-03 18.09% 1.20E-02 18.37%

1.07E-03 0.33% 1.42E-04 0.35% 1.20E-04 0.39% 1.71E-04 0.26%

1.76E-01 53.80% 2.03E-02 50.06% 1.77E-02 57.19% 3.40E-02 52.05%

1.96E-02 5.99% 3.21E-03 7.92% 2.52E-03 8.14% 6.90E-03 10.56%

3.95E-03 1.21% 6.55E-04 1.62% 5.55E-04 1.79% 1.24E-03 1.90%

1.90E-04 0.06% 4.46E-05 0.11% 3.64E-05 0.12% 2.49E-04 0.38%

3.27E-01 4.05E-02 3.09E-02 6.53E-02

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congener 169 was not detected in any samples, so was not included in the TEQ sum.
Table B3-2. Continued

Station

Chemical

PCB077

PCB105

PCB114

PCB118

PCB123

PCB126

PCB156

PCB157

PCB189

Total TEQ

S3-3 Percent of S5-1 Percent of S5-2 Percent of S5-3 Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

8.33E-04 2.01% 1.09E-03 2.14% 2.10E-04 2.07% 8.62E-04 1.86%

3.30E-03 7.97% 4.60E-03 9.02% 8.80E-04 8.66% 4.00E-03 8.64%

1.87E-03 4.51% 2.50E-03 4.90% 4.77E-04 4.69% 2.08E-03 4.49%

7.30E-03 17.64% 8.10E-03 15.89% 1.50E-03 14.76% 7.30E-03 15.76%

1.54E-04 0.37% 2.18E-04 0.43% 3.44E-05 0.34% 1.81E-04 0.39%

2.29E-02 55.33% 2.99E-02 58.66% 6.19E-03 60.92% 2.76E-02 59.60%

4.09E-03 9.88% 3.77E-03 7.39% 7.15E-04 7.04% 3.48E-03 7.50%

9.05E-04 2.19% 7.70E-04 1.51% 1.50E-04 1.47% 7.75E-04 1.67%

4.36E-05 0.11% 3.16E-05 0.06% 4.58E-06 0.05% 3.24E-05 0.07%

4.14E-02 5.10E-02 1.02E-02 4.63E-02

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congener 169 was not detected in any samples, so was not included in the TEQ sum.
Table B3-2. Continued

Station

Chemical

PCB077

PCB105

PCB114

PCB118

PCB123

PCB126

PCB156

PCB157

PCB189

Total TEQ

S5-4 Percent of S5-5 Percent of

TEQ Q Total TEQ TEQ Q Total TEQ

6.32E-04 2.15% 1.82E-04 D 2.22%

1.70E-03 5.78% 7.20E-04 8.78%

1.33E-03 4.50% 3.04E-04 3.70%

3.40E-03 11.56% 1.50E-03 18.28%

1.12E-04 0.38% 2.39E-05 0.29%

1.94E-02 65.93% 4.79E-03 58.39%

2.36E-03 8.00% 5.60E-04 6.83%

4.77E-04 1.62% 1.18E-04 1.44%

2.32E-05 0.08% 6.48E-06 0.08%

2.94E-02 8.20E-03

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congener 169 was not detected in any samples, so was not included in the TEQ sum.
Table B3-3. Mammals TEQs using fish tissue samples collected for the WDNR food chain study

Station 603388 603388 603388

Sample 9413 9414 9415

Species longnose dace longnose dace longnose dace 

Location control control control 

Chemical Mammal TEFs TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

PCB077 0.0001 0.00005 U 0.09% na na

PCB105 0.0001 0.00099 1.80% na na

PCB118 0.0001 0.0025 4.55% 0.00068 na 0.00033 na

PCB126 0.1 0.05 U 90.93% na na

PCB156 0.0005 0.0012 2.18% na na

PCB157 0.0005 0.00025 U 0.45% na na

Total TEQ 0.05499 na na

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B3-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

Total TEQ

603389 603389 603389

9413 9414 9415

longnose dace longnose dace longnose dace 

rochester park rochester park rochester park 

TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

na na 0.0016 0.70%

na na 0.024 10.57%

0.03 na 0.032 na 0.074 32.58%

na na 0.1 U*I 44.03%

na na 0.022 9.69%

na na 0.0055 2.42%

na na 0.2271

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B3-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

Total TEQ

603390 603390 603390

9413 9414 9415

longnose dace longnose dace longnose dace 

esselingen park esselingen park esselingen park 

TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

0.002 1.03% na na

0.026 13.40% na na

0.076 39.18% 0.07 na 0.046 na

0.05 U 25.77% na na

0.027 13.92% na na

0.013 6.70% na na

0.194 na na

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B3-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

Total TEQ

603391 603391 603391

9413 9414 9415

longnose dace longnose dace longnose dace 

between kohler dams between kohler dams between kohler dams

TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

na na 0.0017 1.11%

na na 0.017 11.10%

0.046 na 0.056 na 0.056 36.55%

na na 0.05 U 32.64%

na na 0.019 12.40%

na na 0.0095 6.20%

na na 0.1532

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B3-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

Total TEQ

603388 603388 603388

9404 9405 9406

smallmouth bass smallmouth bass smallmouth bass 

control control control 

TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

na na 0.00005 U 0.10%

na na 0.00014 0.27%

0.00039 na 0.00044 na 0.00076 1.48%

na na 0.05 U 97.18%

na na 0.00025 U 0.49%

na na 0.00025 U 0.49%

na na 0.05145

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B3-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

Total TEQ

603389 603389 603389

9404 9405 9406

smallmouth bass smallmouth bass smallmouth bass 

rochester park rochester park rochester park 

TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

na na 0.0029 1.02%

na na 0.034 11.98%

0.092 na 0.072 na 0.092 32.41%

na na 0.12 U*I 42.27%

na na 0.027 9.51%

na na 0.008 2.82%

na na 0.2839

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B3-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

Total TEQ

603390 603390 603390

9404 9405 9406

smallmouth bass smallmouth bass smallmouth bass 

esselingen park esselingen park esselingen park 

TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

na na 0.0014 1.07%

na na 0.017 12.95%

0.025 na 0.036 na 0.048 36.56%

na na 0.05 U 38.08%

na na 0.0115 8.76%

na na 0.0034 2.59%

na na 0.1313

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B3-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

Total TEQ

603391 603391 603391

9404 9405 9406

smallmouth bass smallmouth bass smallmouth bass 

between kohler dams between kohler dams between kohler dams

TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

na na 0.0041 0.94%

na na 0.043 9.89%

0.083 na 0.072 na 0.12 27.61%

na na 0.2 46.02%

na na 0.0495 11.39%

na na 0.018 4.14%

na na 0.4346

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B3-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

Total TEQ

603388 603388 603388

9410 9411 9412

white sucker white sucker white sucker 

control control control 

TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

na na 0.00005 U 0.10%

na na 0.00032 0.62%

0.00013 na 0.00016 na 0.0011 2.12%

na na 0.05 U 96.21%

na na 0.00025 U 0.48%

na na 0.00025 U 0.48%

na na 0.05197

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B3-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

Total TEQ

603389 603389 603389

9410 9411 9412

white sucker white sucker white sucker 

rochester park rochester park rochester park 

TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

na na 0.0011 0.71%

na na 0.013 8.44%

0.037 na 0.04 na 0.031 20.12%

na na 0.095 U*I 61.65%

na na 0.011 7.14%

na na 0.003 1.95%

na na 0.1541

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B3-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

Total TEQ

603390 603390 603390

9410 9411 9412

white sucker white sucker white sucker 

esselingen park esselingen park esselingen park 

TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

na na 0.00064 0.74%

na na 0.0061 7.09%

0.014 na 0.014 na 0.02 23.26%

na na 0.05 U 58.15%

na na 0.0065 7.56%

na na 0.00275 3.20%

na na 0.08599

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B3-3. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

Total TEQ

603391 603391 603391

9410 9411 9412

white sucker white sucker white sucker 

between kohler dams between kohler dams between kohler dams

TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ TEQ Q

Percent of 

Total TEQ

na na 0.00078 0.79%

na na 0.0083 8.45%

0.025 na 0.045 na 0.026 26.48%

na na 0.05 U 50.93%

na na 0.0095 9.68%

na na 0.0036 3.67%

na na 0.09818

All concentrations in ug/kg wet weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the Percent of Total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.

5) na - not applicable when all TEF congeners are not measured.
Table B3-4. Mammal TEQs using sediment data collected for the WDNR food chain study

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

Total TEQ

603366 603367 603368

6D 6E 5A 

sediment sediment sediment 

camp marina Percent of camp marina Percent of kiwanis park Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.00079 2.94% 0.0004 2.50% 0.00073 3.12%

0.0029 10.78% 0.0015 9.38% 0.0034 14.54%

0.0053 19.71% 0.0026 16.26% 0.0059 25.24%

0.015 U*I 55.78% 0.01 U 62.52% 0.01 U 42.77%

0.00225 8.37% 0.00115 7.19% 0.00265 11.33%

0.00065 2.42% 0.000345 2.16% 0.0007 2.99%

0.02689 0.015995 0.02338

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.
Table B3-4. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

Total TEQ

603369 603370 603371

5B 5C 5D 

sediment sediment sediment 

kiwanis park Percent of kiwanis park Percent of kiwanis park Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.00075 3.13% 0.00046 2.57% 0.00062 2.97%

0.003 12.53% 0.002 11.16% 0.0029 13.90%

0.0055 22.96% 0.0035 19.53% 0.0048 23.00%

0.0115 U*I 48.02% 0.01 U 55.80% 0.01 U 47.92%

0.00245 10.23% 0.0015 8.37% 0.00195 9.34%

0.00075 3.13% 0.00046 2.57% 0.0006 2.87%

0.02395 0.01792 0.02087

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.
Table B3-4. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

Total TEQ

603372 603373 603374

3A 3B 3C 

sediment sediment sediment 

between kohler dams Percent of between kohler dams Percent of between kohler dams Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.001 2.14% 0.00086 2.57% 0.00052 2.57%

0.0047 10.06% 0.0034 10.18% 0.0019 9.40%

0.0089 19.06% 0.0068 20.35% 0.004 19.78%

0.027 57.82% 0.018 U*I 53.88% 0.0115 U*I 56.87%

0.00395 8.46% 0.00335 10.03% 0.00175 8.65%

0.00115 2.46% 0.001 2.99% 0.00055 2.72%

0.0467 0.03341 0.02022

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.
Table B3-4. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

Total TEQ

603375 603376 603377

3D 3E 2A 

sediment sediment sediment 

between kohler dams Percent of between kohler dams Percent of rochester park Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.0009 3.36% 0.00098 2.72% 0.0007 2.71%

0.0033 12.34% 0.0037 10.25% 0.0029 11.24%

0.0079 29.53% 0.0088 24.39% 0.0059 22.87%

0.01 U 37.38% 0.0175 U*I 48.50% 0.013 U*I 50.39%

0.00355 13.27% 0.00395 10.95% 0.0025 9.69%

0.0011 4.11% 0.00115 3.19% 0.0008 3.10%

0.02675 0.03608 0.0258

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.
Table B3-4. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

Total TEQ

603378 603379 603380

2B 2C 2D 

sediment sediment sediment 

rochester park Percent of rochester park Percent of rochester park Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.0015 2.65% 0.00073 3.16% 0.0027 2.27%

0.0066 11.66% 0.0025 10.81% 0.01 8.42%

0.012 21.20% 0.006 25.94% 0.029 24.43%

0.03 U*I 53.00% 0.011 U*I 47.56% 0.06 U*I 50.55%

0.005 8.83% 0.00225 9.73% 0.013 10.95%

0.0015 2.65% 0.00065 2.81% 0.004 3.37%

0.0566 0.02313 0.1187

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.
Table B3-4. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

Total TEQ

603381 603392 603393

2E 1A 1B 

sediment sediment sediment 

rochester park Percent of control Percent of control Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.003 3.06% 0.00001 U 0.09% 0.00001 U 0.10%

0.016 16.32% 0.00001 U 0.09% 0.000023 0.23%

0.029 29.58% 0.0000225 U 0.20% 0.000054 0.53%

0.0325 U*I 33.15% 0.011 U 98.72% 0.01 U 98.16%

0.0135 13.77% 0.00005 U 0.45% 0.00005 U 0.49%

0.00405 4.13% 0.00005 U 0.45% 0.00005 U 0.49%

0.09805 0.0111425 0.010187

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.
Table B3-4. Continued

Station

Sample

Species

Location

Chemical

PCB077

PCB105

PCB118

PCB126

PCB156

PCB157

Total TEQ

603394 603395 603396

1C 1D 1E 

sediment sediment sediment 

control Percent of control Percent of control Percent of

TEQ Q Total TEQ TEQ Q Total TEQ TEQ Q Total TEQ

0.00001 U 0.10% 0.00001 U 0.10% 0.00001 U 0.10%

0.00001 U 0.10% 0.000034 0.33% 0.000028 0.27%

0.000046 0.45% 0.000082 0.80% 0.000076 0.74%

0.01 U 98.37% 0.01 U 97.79% 0.01 U 97.90%

0.00005 U 0.49% 0.00005 U 0.49% 0.00005 U 0.49%

0.00005 U 0.49% 0.00005 U 0.49% 0.00005 U 0.49%

0.010166 0.010226 0.010214

All concentrations in ug/kg dry weight.

1) For PCB congeners that were not detected, 1/2 the detection limit was used if that congener was detected in another sample.

 This value contributes to the total TEQ sum.

2) PCB congener 81 was not analyzed in any samples.

3) PCB congeners 123 and 169 were not detected in any samples, so were not included in the TEQ sum.

4) PCB congeners 77, 105, 126, 156 and 157 were only analyzed in some samples.
APPENDIX C

Laboratory Report

Freshwater Sediment Toxicity Testing Program
October 1997

LABORATORY REPORT

PREPARED FOR: PREPARED BY:

EVS Environment Consultants

Seattle, WA

North Vancouver, BC

NOAA - Sheboygan, WI

Freshwater Sediment Toxicity

Testing Program
FRESHWATER SEDIMENT TOXICITY TESTING

PROGRAM

NOAA - SHEBOYGAN, WI

LABORATORY REPORT

Prepared for

EVS Environment Consultants

200 West Mercer Street

Suite 403

Seattle WA 98119

Prepared by

EVS Environment Consultants

195 Pemberton Avenue

North Vancouver, BC

V7P 2R4

EVS Project No.

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ACKNOWLEDGEMENTS

Freshwater sediment toxicity testing was conducted by George Yang, Lori Suffredine, May

Lee, Betty Yung, Patricia Haynes, Stefan Santos, Elizabeth Thys, Bushra Jamil, Amanda

Ward, Andy Diewald, Edmund Canaria, Alice Yang, Kevin Bahr, Camille Merchant, Micaele

Madison, Jan Widmer and Julianna Galfi Kalocai. The statistical analyses and report

preparation were conducted by Patricia Haynes. Quality assurance/quality control (QA/QC)

review of the data was conducted by Cathy McPherson. The report was reviewed by Jennifer

Stewart. Kristen Ramsden and Jackie Gelling were responsible for word processing.
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1.0

INTRODUCTION

Eighteen sediment samples were received by the EVS Laboratory on August 20 and 22,

1997. These samples were collected between August 12 and 18, 1997. Toxicity tests were

conducted on all samples using the freshwater midge, Chironomus tentans and the juvenile

amphipod, Hyalella azteca. This report describes the results of these tests. Chain-of-Custody

forms submitted with the samples are provided in Appendix A. Test data and statistical

printouts are provided in Appendices B and C, respectively.

The samples were each shipped in one 2-L glass containers which were then homogenized

thoroughly. Prior to test initiation the sediments were stored at 4EC in the dark.

Both the midge test and the amphipod test were initiated with cultured organisms obtained

from a commercial supplier. The exposure duration was 10 days for both tests, the endpoints

were survival and growth.

1.1 QUALITY ASSURANCE/QUALITY CONTROL (QA/QC)

This study was conducted under our comprehensive QA/QC Program to ensure full

documentation and minimize possible errors in computation and reporting of results. The

details of our QA/QC Program are documented in our Laboratory QA/QC Manual which

describes all aspects of our program, including information on general laboratory

procedures, sample handling, toxicity test procedures, data interpretation and management,

and documentation of results. The following general QA/QC guidelines apply to all toxicity

tests: use of negative controls, use of positive controls, replication, instrument calibration,

water quality maintenance and record-keeping, and use of standard operating procedures

(SOP). To ensure the highest quality of data and reporting, all data and statistical analyses

for each toxicity test are reviewed by a member of our QA/QC Committee prior to the report

being released.
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2.0

10-d MIDGE (Chironomus tentans) SEDIMENT TOXICITY TEST

2.1 METHODS

Ten-day water-renewal toxicity tests using the freshwater midge, Chironomus tentans, were

conducted according to methods described in ASTM (1996). Test organisms were obtained

from a commercial supplier in Wisconsin and were third instar at test initiation. The

chironomids were cultured in moderately hard water (80 - 100 mg/L as CaCO3) at 23 "

1EC under a 16:8 h light:dark photoperiod.

Tests were conducted in 300 mL glass beakers. Six replicates, plus one sacrificial replicate

for Day 0 ammonia analysis were prepared for each sample, including the negative control. 

One of the six replicates was used for monitoring daily water quality and Day 10 ammonia

analysis. Moderately hard reconstituted water was used for testing. Reagent-grade chemicals

were added to dechlorinated water to achieve a final hardness of 80 - 100 mg/L as CaCO3.

The toxicity tests were conducted at 23 " 1EC, under a 16:8 h light:dark photoperiod, in a

constant environment chamber.

Sediments were distributed to the test containers the day prior to test initiation (Day -1). A

100-mL volume of test sediment was added to each beaker. Approximately 175 mL of

moderately hard water (80 - 100 mg/L CaCO3) was added gently to each beaker using a

turbulence reducer to minimize disturbing the sediments. Water renewal by volume was

initiated the same day, the beakers were then covered with clean plexiglass and left to settle

overnight. On Day 0, each beaker was seeded with 10 randomly selected chironomids of

similar size (approximately 7 mm in length). Prior to seeding, the head capsule widths were

measured on 20 additional larvae to ensure that 50% of the midges were in third instar. The

test was not aerated until Day 3, when the dissolved oxygen dropped below 3.4 mg/L (40%

saturation). The test organisms were fed a diet of 1.5 mL of Tetra-Min fish food mixture

daily.

Two additional sets of 10 larvae were set aside for determination of initial dry weight

(biomass). Water quality parameters (temperature, pH, dissolved oxygen, and conductivity)

were measured daily for each treatment, in a replicate designated for measuring water

quality parameters. Two overlying water renewals (by volume) were completed daily.

Hardness and alkalinity were measured in composite samples of overlying water from each

treatment on Days 0 and 10, using methods described in APHA (1995). Ammonia

concentrations in interstitial water and sulfide concentrations in overlying water were

measured on Days 0 and 10.

Total ammonia concentrations were measured in the interstitial water from each sample,
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using the Salicylate Method (Hach Company, 1992). Interstitial water was collected by

centrifugation of a small portion of sediment from the sacrificial replicate. Appropriate

reagents were added and the absorbance was measured for each sample using a

spectrophotometer. The absorbance reading was compared to a standard curve to determine

the total ammonia concentration (mg/L N) in each sample. Composite subsamples of

overlying water were collected from each sample and analyzed for total sulfides using an ion

specific electrode.

The test was terminated after 10 d when the sediments were sieved and the live and dead

larvae were removed and counted. For the test to be considered valid, mean negative control

 survival had to be at least 70% and average size of C. tentans in the negative control be at

least 0.6 mg at the end of test (ASTM, 1996). Surviving larvae (excluding pupae and flies)

from each replicate were transferred to pre-weighed aluminum pans and dried at 60EC for

24 hours for determination of total dry weight. The larvae were weighed to an accuracy of

0.1 mg to obtain final dry weights. Mean individual dry weights were obtained by dividing

the final dry weight of each replicate by the number of larvae weighed.

Statistical analyses were performed using the TOXCALC computer program (Tidepool

Scientific Software, 1994). Survival and dry weight data were analysed separately. The

survival and dry weight data were tested for normality and homogeneity of variance. If the

survival data did not pass the tests for normality and homogeneity of variance, then the data

were transformed. If transformation did not allow the data to pass these tests, untransformed

data were used. Dry weight data were not transformed. Homoscedastic t-tests or the nonparametric Heteroscedastic t-tests were then performed to determine if any of the test

sediments were significantly different (p#0.05) from the negative control with respect to

survival or dry weight. Samples significantly different with respect to survival were omitted

from dry weight analyses.

A concurrent 96-h LC50 positive (toxic) control test was conducted with the reference

toxicant potassium chloride (KCl) to assess the health and sensitivity of the chironomids.

Potassium chloride solutions were prepared from reagent grade KCl and diluted with

moderately hard reconstituted water to obtain the specified concentrations. The test

consisted of five concentrations (1.25, 2.5, 5, 10 and 20 g/L KCl) with three replicates each.

Ten randomly selected larvae of third instar testing size were exposed to each treatment.

Test containers were 300-mL beakers, each containing 150 mL of test solution and a

monolayer of control sediment for burrowing. Water quality measurements were recorded

at the beginning and end of the test, and temperature and survival were recorded daily. The

chironomids were fed 1.25 mL of Tetra-Min mixture on Day 0 and 2. Beakers were not

aerated during testing.

2.2 RESULTS

Results of the sediment toxicity test is summarized in Table 2-1. Raw data and statistical
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printouts are provided in Appendix B.

Mean survival in the negative control was 96.7%. Mean survival in the samples ranged from

0 to 95.0%. Mean dry weight in the negative control was 0.81 mg/larva. Mean dry weight in

the samples ranged from 1.44 to 2.16 mg/larva.

With respect to survival, Heteroscedastic t-tests indicated there were significant differences

(p#0.05).

With respect to dry weight, Homoscedastic t-tests indicated there were no significant

differences (p#0.05) between samples and the negative control.

2.3 QUALITY ASSURANCE/QUALITY CONTROL (QA/QC)

Mean survival responses in the negative control met the criterion for test acceptability as

outlined in ASTM (1996).

Seven replicates were initially requested for testing, however at the time of seeding only six

replicates were seeded due to a shortage of animals received from the supplier. Client was

previously notified of this deviation.

Water quality parameters measured during the 10-d exposure period were in the following

ranges: temperature, 22.0 - 23.0EC; pH 7.0 - 8.3; dissolved oxygen, 3.0 - 8.6 mg/L and

conductivity 280 - 500 F mhos/cm. Hardness measurements ranged from 106 - 166 mg/L as

CaCO3 on Day 0 and from 104 - 152 mg/L as CaCO3 on Day 10. Alkalinity measurements

ranged from 67 - 136 mg/L as CaCO3 on Day 0 and 86 - 124 mg/L as CaCO3 on Day 10.

Dissolved Oxygen measurements were generally $ 40% saturation (3.4 mg/L) during the

10-d exposure, however there were some exceptions. Aeration was checked daily and

adjusted as needed. These low oxygen measurements do not appear to have affected the test

results. Sulfide levels in overlying water and interstitial ammonia concentrations measured

during the 10-d exposure are summarized in Table 2-2.

The 96-h LC50 value for the KCl reference toxicant (initiated September 5, 1997) was

determined using the TOXCALC computer program. The 96-h LC50 value for KCl was

5.4 g/L KCl (95% confidence limits: 4.2 and 6.8 g/L KCl) which is within the laboratory

range of 4.5 " 3.0 g/L KCl (mean " 2SD). Water quality parameters measured during

the 96-h exposure period were within the following ranges: temperature, 22.0 - 23.0EC;

pH, 7.4 - 8.0; dissolved oxygen, 4.8 - 8.6 mg/L; and conductivity, 200 - 47,000 F mhos/cm.

Table 2-1. Summary of Chironomus tentans sediment toxicity test results
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Mean " SD

Sample ID SURVIVAL (%)1 DRY WEIGHT (mg)2

Negative Control 96.7 " 5.2 0.81 " 0.19

SR-1-SS-B 95.0 " 5.5 1.90 " 0.18

SR-2-SS-B 68.3 " 18.4* 1.71 " 0.25

SR-3-SS-B 81.7 " 17.2* 2.16 " 0.25

SR-4-SS-B 88.3 " 7.5* 1.62 " 0.18

SR-7-SS-F 0.0 " 0.0* -

SR-8-SS-F 91.7 " 7.5 2.01 " 0.18

SR-9-SS-F 77.5 " 9.6* 1.96 " 0.18

SR-10-SS-F 88.3 " 7.5* 2.04 " 0.22

SR-11-SS-F 78.3 " 18.4* 2.14 " 0.41

SR-12-SS-F 91.7 " 9.8 1.54 " 0.19

SR-13-SS-F 85.0 " 13.8 1.58 " 0.14

SR-14-SS-F 76.7 " 22.5* 1.62 " 0.17

SR-15-SS-F 83.3 " 31.4 1.68 " 0.31

SR-16-SS-F 86.7 " 17.5 1.44 " 0.30

SR-17-SS-F 73.3 " 26.6* 1.70 " 0.34

SR-18-SS-F 93.3 " 8.2 1.49 " 0.15

SR-19-SS-F 81.7 " 17.2* 1.60 " 0.27

SR-20-SS-F 83.3 " 21.6 1.59 " 0.43

1 Asterisks (*) indicate samples significantly different (p#0.05) from the negative control.

2 Samples significantly different with respect to survival were omitted from dry weight statistical analyses.
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Table 2-2. Summary of Chironomus tentans interstitial ammonia

and overlying sulfide results

TOTAL INTERSTITIAL AMMONIA

(mg/L N)

TOTAL SULFIDES

(mg/L S)

SAMPLE ID DAY 0 DAY 10 DAY 0 DAY 10

Negative Control < 0.1 1.1 0.000 0.000

SR-1-SS-B 7.0 0.3 0.000 0.000

SR-2-SS-B 17.3 0.2 0.000 0.000

SR-3-SS-B 11.6 1.9 0.000 0.000

SR-4-SS-B 26.9 7.1 0.000 0.000

SR-7-SS-F 5.9 2.7 0.000 0.000

SR-8-SS-F 16.4 2.1 0.000 0.000

SR-9-SS-F 9.6 4.2 0.000 0.000

SR-10-SS-F 27.8 0.9 0.000 0.000

SR-11-SS-F 22.8 <0.1 0.000 0.001

SR-12-SS-F 16.4 1.8 0.000 0.000

SR-13-SS-F 25.1 7.1 0.000 0.000

SR-14-SS-F 31.0 5.5 0.000 0.000

SR-15-SS-F 20.5 6.7 0.000 0.000

SR-16-SS-F 16.8 3.8 0.000 0.000

SR-17-SS-F 12.3 8.4 0.000 0.000

SR-18-SS-F 24.6 7.1 0.000 0.000

SR-19-SS-F 25.5 4.7 0.000 0.000

SR-20-SS-F 19.1 3.8 0.000 0.000
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3.0

10-d AMPHIPOD (Hyalella azteca) SEDIMENT TOXICITY TEST

3.1 METHODS

Ten-day water-renewal toxicity tests using the freshwater amphipod Hyalella azteca were

conducted according to methods described in ASTM (1996). Test organisms were obtained

from a commercial supplier in New Hampshire and were 14 d old at test initiation. The

amphipods were cultured in moderately hard water (80 - 100 mg/L as CaCO3) at 23 " 1EC

under a 16:8 h light:dark photoperiod. The amphipods were fed a combination of algae

(Selenastrum capricornutum), d-YCT (yeast/cereal flakes/digested trout food), and TetraMin mixture every second day during the holding period.

Tests were conducted in 300 mL glass beakers. Eight replicates were prepared for each

sample, including the negative control. Of the 8 replicates, one sacrificial replicate was used

for Day 0 interstitial ammonia analysis and one for monitoring daily water quality and Day

10 interstitial ammonia. Moderately hard reconstituted water was used for testing. Reagentgrade chemicals were added to dechlorinated water to achieve a final hardness of 80 - 100

mg/L as CaCO3. The toxicity tests were conducted at 23 " 1EC, under a 16:8 h light:dark

photoperiod, in a constant environment chamber.

Sediments were distributed to the test containers the day prior to test initiation (Day -1). A

100-mL volume of test sediment was added to each beaker. Approximately 175 mL of

moderately hard water (80 - 100 mg/L CaCO3) was added gently to each beaker using a

turbulence reducer to minimize disturbing the sediments. Water renewal by volume was

initiated the same day, the beakers were then covered with clean plexiglass and left to settle

overnight. On Day 0, each beaker was seeded with 10 randomly selected amphipods of

similar size (approximately 2 - 3 mm in length). The test was not aerated until Day 8 when

the dissolved oxygen dropped below 3.4 mg/L (40% saturation). Test organisms were fed a

diet of 1.5 mL of d-YCT daily.

Two additional sets of 10 amphipods were set aside for determination of initial dry weight

(biomass). Water quality parameters (temperature, pH, dissolved oxygen, and conductivity)

were measured daily for each treatment, in a replicate designated for measuring water

quality parameters. Two overlying water renewals (by volume) were completed daily.

Hardness and alkalinity were measured in composite samples of overlying water from each

treatment on Days 0 and 10, using methods described in APHA (1995). Ammonia

concentrations in interstitial water and sulfide concentrations in overlying water were

measured on Days 0 and 10.

Total ammonia concentrations were measured in the interstitial water from each sample,
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using the Salicylate Method (Hach Company, 1992). Interstitial water was collected by

centrifugation of a small portion of sediment from the sacrificial replicate. Appropriate

reagents were added and the absorbance was measured for each sample using a

spectrophotometer. The absorbance reading was compared to a standard curve to determine

the total ammonia concentration (mg/L N) in each sample. Composite subsamples of

overlying water were collected from each sample and analyzed for total sulfides using an ion

specific electrode.

The test was terminated after 10 d when the sediments were sieved and the live and dead

amphipods were removed and counted. Amphipods were considered dead when there was

no response to physical stimulation. Missing amphipods were assumed to have died and

decomposed prior to the termination of the test. For the test to be considered valid, mean

negative control survival had to be at least 80% (ASTM, 1996). Surviving amphipods from

each replicate were transferred to pre-weighed aluminum pans and dried at 60 EC for

24 hours for determination of total dry weight. The amphipods were weighed to an accuracy

of 0.1 mg to obtain final dry weights. Mean individual dry weights were obtained by

dividing the final dry weight of each replicate by the number of amphipods weighed.

Statistical analyses were performed using the TOXCALC computer program (Tidepool

Scientific Software, 1994). Survival and dry weight data were analysed separately. The

survival and dry weight data were tested for normality and homogeneity of variance. If the

survival data did not pass the tests for normality and homogeneity of variance, then the data

were transformed. If transformation did not allow the data to pass these tests, untransformed

data were used. Dry weight data were not transformed. Homoscedastic t-tests or nonparametric Heteroscedastic t-tests were then performed to determine if any of the test

sediments were significantly different (p#0.05) from then negative control with respect to

survival or dry weight. Samples significantly different with respect to survival were omitted

from the dry weight analysis.

A concurrent 96-h LC50 positive (toxic) control test was conducted with the reference

toxicant, zinc (prepared from zinc sulphate, ZnSO4$7H2O) to assess the health and

sensitivity of the amphipods. Zinc stock solution was prepared from reagent grade zinc

sulphate and diluted with moderately hard reconstituted water to obtain the specified

concentrations. The test consisted of five concentrations (32, 56, 100, 180 and 320 F g/L as

Zn) with three replicates each. Ten randomly selected amphipods (2 - 3 mm in length) were

exposed to each treatment. The test containers were 300-mL beakers each containing 200

mL of test solution and a small 110 F g piece of nylon mesh as substrate. Water quality

measurements were recorded at the beginning and end of the test, and temperature and

survival were recorded daily. The amphipods were fed 0.5 mL of d-YCT on Days 0 and 2.

Beakers were not aerated during testing.

3.2 RESULTS

Results of the sediment toxicity test is summarized in Table 3-1. Raw data and statistical
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printouts are provided in Appendix C.

Mean survival in the negative control was 96.7%. Mean survival in the samples ranged from

0 to 95.0%. Mean dry weight in the negative control was 0.12 mg/amphipod. Mean dry

weight in the samples ranged from 0.09 to 0.19 mg/amphipod.

With respect to survival, Heteroscedastic t-tests indicated there were significant differences

(p#0.05).

With respect to dry weight, Homoscedastic t-tests indicated no significant differences

between the samples and the negative control.

3.3 QUALITY ASSURANCE/QUALITY CONTROL (QA/QC)

Mean survival responses in the negative control met the criterion for test acceptability as

outlined in ASTM (1996).

Please note that Samples SR-9-SS-F, SR-13-SS-F, and SR-15-SS-F, had one replicate where

the survival varied from the other replicates. Therefore statistical analysis was performed

including and excluding this replicate of each sample. The results from comparing both

statistical analysis, demonstrated that there was no statistical difference in each sample

analysis regardless of whether the replicate was included or not.

Water quality parameters measured during the 10-d exposure period were within the

following ranges: temperature, 22.0 - 24.0EC; pH, 7.1 - 8.3; dissolved oxygen,

2.4 - 8.7 mg/L; and conductivity, 300 - 600 F mhos/cm. Hardness measurements ranged

from 94 - 136 mg/L as CaCO3 on Day 0 and 112 - 156 mg/L as CaCO3 on Day 10.

Alkalinity measurements ranged from 64 - 112 mg/L as CaCO3 on Day 0 and 76 - 118 mg/L

as CaCO3 on Day 10. Dissolved oxygen measurements were generally $ 40% saturation

(3.4 mg/L) during the 10-d exposure, however there were some exceptions. Aeration was

checked daily and adjusted as needed. These low oxygen measurements do not appear to

affect the test results. Sulfide levels in overlying water and interstitial ammonia

concentrations measured during the 10-d exposure for each batch of testing are summarized

in Table 3-2.

The 96-h LC50 for the zinc reference toxicant (initiated August 26, 1997) was determined

using the TOXCALC computer program. The 96-h LC50 value for zinc was 145 F g/L Zn

(95% confidence limits: 130 and 162 F g/L Zn). A laboratory mean has not yet been

generated due to insufficient data points for this method. However, this value is consistent

with values obtained for our in-house cultures. Water quality parameters measured during

the 96-h exposure period were within the following ranges: temperature, 22.5 - 24.0EC; pH,

7.7 - 8.0; dissolved oxygen, 7.4 - 8.5 mg/L; and conductivity, 310 - 320 F mhos/cm.
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Table 3-1. Summary of Hyalella azteca sediment toxicity test results

Sample ID

Mean " SD

SURVIVAL (%)1 DRY WEIGHT (mg)2

Negative Control 96.7 " 5.2 0.12 " 0.03

SR-1-SS-B 95.0 " 8.4 0.16 " 0.02

SR-2-SS-B 86.7 " 10.3* 0.14 " 0.02

SR-3-SS-B 68.3 " 21.4* 0.17 " 0.04

SR-4-SS-B 46.7 " 12.1* 0.18 " 0.09

SR-7-SS-F 0.0 " 0.0* -

SR-8-SS-F 91.7 " 7.5 0.19 " 0.08

SR-9-SS-F 75.0 " 41.8 (90.0 " 22.4) 0.17 " 0.04

SR-10-SS-F 66.7 " 25.0* 0.17 " 0.06

SR-11-SS-F 85.0 " 8.4* 0.13 " 0.04

SR-12-SS-F 88.3 " 11.7 0.12 " 0.03

SR-13-SS-F 66.1 " 28.0* (60.0 " 26.5*) 0.09 " 0.02

SR-14-SS-F 78.3 " 14.7* 0.10 " 0.03

SR-15-SS-F 65.0 " 38.9 (78.0 " 24.9) 0.15 " 0.03

SR-16-SS-F 61.7 " 28.6* 0.15 " 0.03

SR-17-SS-F 86.7 " 12.1 0.16 " 0.05

SR-18-SS-F 78.3 " 13.3* 0.13 " 0.02

SR-19-SS-F 60.0 " 37.4* 0.19 " 0.06

SR-20-SS-F 63.3 " 25.8* 0.14 " 0.04

1 Asterisks (*) indicate samples significantly different (p#0.05) from the negative control (Bracketed

survival values indicate anomalous replicate removed from statistical analysis. See text for details).

2 Samples significantly different with respect to survival were omitted from dry weight statistical analyses.
9/575-37.10

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Table 3-2. Summary of Hyalella azteca interstitial ammonia

and overlying sulfide results

SAMPLE ID

TOTAL INTERSTITIAL AMMONIA

(mg/L N)

TOTAL SULFIDES

(mg/L S)

DAY 0 DAY 10 DAY 0 DAY 10

Negative Control <0.1 1.5 0.000 0.000

SR-1-SS-B 10.7 8.2 0.000 0.000

SR-2-SS-B 16.3 10.9 0.000 0.000

SR-3-SS-B 11.1 10.4 0.000 0.000

SR-4-SS-B 29.5 17.2 0.000 0.000

SR-7-SS-F 7.5 5.0 0.000 0.000

SR-8-SS-F 17.8 10.4 0.015 0.000

SR-9-SS-F 13.3 5.9 0.000 0.000

SR-10-SS-F 23.6 19.1 0.000 0.000

SR-11-SS-F 21.0 15.4 0.015 0.001

SR-12-SS-F 16.0 10.4 0.000 0.000

SR-13-SS-F 25.4 16.3 0.000 0.000

SR-14-SS-F 27.7 14.5 0.000 0.000

SR-15-SS-F 28.6 20.0 0.000 0.000

SR-16-SS-F 20.1 15.4 0.000 0.000

SR-17-SS-F 14.2 9.1 0.000 0.000

SR-18-SS-F 17.2 10.4 0.000 0.000

SR-19-SS-F 21.0 15.4 0.000 0.000

SR-20-SS-F 18.5 15.0 0.000 0.000
9/575-37.10

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4.0

REFERENCES

APHA (American Public Health Association). 1995. Standard Methods for the Examination

of Water and Wastewater. 19th edition. American Public Health Association,

American Water Works Association and Water Environment Federation,

Washington, DC.

ASTM (American Society for Testing and Materials). 1996. Standard test methods for

measuring the toxicity of sediment-associated contaminants with fresh water

invertebrates. Method E1706-95b. In: 1996 Annual Book of ASTM Standards, Water

and Environmental Technology, Volume 11.05. American Society for Testing and

Materials, Philadelphia, PA.

Hach Company. 1992. Water Analyses Handbook: Second Edition. Hach Company,

Loveland, CO. 831 pp.

Tidepool Scientific Software. 1994. TOXCALC: Comprehensive Toxicity Data Analysis

and Database Software, Version 5.0. Tidepool Scientific Software, McKinleyville,

CA. 80 pp.

U.S. EPA (U.S. Environmental Protection Agency). 1994. Methods for measuring the

toxicity and bioaccumulation of sediment-associated contaminants with freshwater

invertebrates. Office of Research and Development. U.S. Environmental Protection

Agency, Duluth, MN. EPA/600/R-94/024. 131 pp.
APPENDIX A

Chain-of-Custody Forms







APPENDIX B

10-d C. tentans Sediment Toxicity Test

Raw Data

































APPENDIX C

10-d H. azteca Sediment Toxicity Test

Raw Data

































APPENDIX D

Benthic Community Data
EVS CONSULTANTS 20% SORTING QA/QC LOG Page 1 of 3

Environmental Services

EVS PROJECT #: 2/575-37.11 

PROJECT NAME: SHEBOYGAN RIVER 

#QA % Sorting

Sample I.D. Organisms Total Abund. Efficiency P / F Comments

1 SR-1-BC-B-1 0 189 100.0 P 0 QA/QC ORGANISMS

2 SR-1-BC-B-2 0 151 100.0 P 0 QA/QC ORGANISMS

3 SR-1-BC-B-3 0 338 100.0 P 0 QA/QC ORGANISMS

4 SR-1-BC-B-4 0 241 100.0 P 0 QA/QC ORGANISMS

5 SR-1-BC-B-5 0 132 100.0 P 0 QA/QC ORGANISMS

6 SR-2-BC-B-1 0 58 100.0 P 0 QA/QC ORGANISMS

7 SR-2-BC-B-2 0 75 100.0 P 0 QA/QC ORGANISMS

8 SR-2-BC-B-3 0 171 100.0 P 0 QA/QC ORGANISMS

9 SR-2-BC-B-4 0 87 100.0 P 0 QA/QC ORGANISMS

10 SR-2-BC-B-5 0 209 100.0 P 0 QA/QC ORGANISMS

11 SR-3-BC-B-1 1 221 97.7 P Oligs. found In QA/QC

12 SR-3-BC-B-2 0 28 100.0 P 0 QA/QC ORGANISMS

13 SR-3-BC-B-3 1 289 98.3 P Oligs. found In QA/QC

14 SR-3-BC-B-4 1 327 98.5 P Oligs. found In QA/QC

15 SR-3-BC-B-5 2 219 95.4 P Oligs. found In QA/QC

16 SR-4-BC-B-1 0 102 100.0 P 0 QA/QC ORGANISMS

17 SR-4-BC-B-2 1 107 95.3 P Oligs. found In QA/QC

18 SR-4-BC-B-3 0 102 100.0 P 0 QA/QC ORGANISMS

19 SR-4-BC-B-4 0 100 100.0 P 0 QA/QC ORGANISMS

20 SR-4-BC-B-5 1 135 96.3 P Oligs. found In QA/QC

21 SR-7-BC-F-1 0 82 100.0 P 0 QA/QC ORGANISMS

22 SR-7-BC-F-2 0 49 100.0 P 0 QA/QC ORGANISMS

23 SR-7-BC-F-3 0 30 100.0 P 0 QA/QC ORGANISMS

24 SR-7-BC-F-4 0 22 100.0 P 0 QA/QC ORGANISMS

25 SR-7-BC-F-5 0 5 100.0 P 0 QA/QC ORGANISMS

26 SR-8-BC-F-1 1 589 99.2 P Oligs. found In QA/QC

27 SR-8-BC-F-2 5 617 96.0 P Oligs. found In QA/QC

28 SR-8-BC-F-3 2 249 96.0 P Oligs. found In QA/QC

29 SR-8-BC-F-4 3 419 96.4 P Oligs. found In QA/QC

30 SR-8-BC-F-5 5 555 95.5 P Oligs. found In QA/QC

31 SR-9-BC-F-1 2 374 97.3 P Oligs. found In QA/QC

32 SR-9-BC-F-2 2 354 97.2 P Oligs. found In QA/QC

33 SR-9-BC-F-3 2 367 97.3 P Oligs. found In QA/QC

P/F = Pass/Fail
EVS CONSULTANTS 20% SORTING QA/QC LOG Page 2 of 3

Environmental Services

EVS PROJECT #: 2/575-37.11 

PROJECT NAME: SHEBOYGAN RIVER 

#QA % Sorting

Sample I.D. Organisms Total Abund. Efficiency P / F Comments

34 SR-9-BC-F-4 2 541 98.2 P Oligs. found In QA/QC

35 SR-9-BC-F-5 3 520 97.1 P Oligs. found In QA/QC

36 SR-10-BC-F-1 0 432 100.0 P 0 QA/QC ORGANISMS

37 SR-10-BC-F-2 0 214 100.0 P 0 QA/QC ORGANISMS

38 SR-10-BC-F-3 0 302 100.0 P 0 QA/QC ORGANISMS

39 SR-10-BC-F-4 0 292 100.0 P 0 QA/QC ORGANISMS

40 SR-10-BC-F-5 0 254 100.0 P 0 QA/QC ORGANISMS

41 SR-11-BC-F-1 2 378 97.4 P Oligs. found In QA/QC

42 SR-11-BC-F-2 1 259 98.1 P Oligs. found In QA/QC

43 SR-11-BC-F-3 0 183 100.0 P 0 QA/QC ORGANISMS

44 SR-11-BC-F-4 1 329 98.5 P Oligs. found In QA/QC

45 SR-11-BC-F-5 2 342 97.1 P Oligs. found In QA/QC

46 SR-12-BC-F-1 0 371 100.0 P 0 QA/QC ORGANISMS

47 SR-12-BC-F-2 1 385 98.7 P Oligs. found In QA/QC

48 SR-12-BC-F-3 1 381 98.7 P Oligs. found In QA/QC

49 SR-12-BC-F-4 2 461 97.8 P Oligs. found In QA/QC

50 SR-12-BC-F-5 0 356 100.0 P 0 QA/QC ORGANISMS

51 SR-13-BC-F-1 1 1714 99.7 P Oligs. found In QA/QC

52 SR-13-BC-F-2 0 1446 100.0 P 0 QA/QC ORGANISMS

53 SR-13-BC-F-3 0 946 100.0 P 0 QA/QC ORGANISMS

54 SR-13-BC-F-4 1 648 99.2 P Oligs. found In QA/QC

55 SR-13-BC-F-5 1 688 99.3 P Oligs. found In QA/QC

56 SR-14-BC-F-1 3 421 96.4 P Oligs. found In QA/QC

57 SR-14-BC-F-2 1 342 98.5 P Oligs. found In QA/QC

58 SR-14-BC-F-3 0 259 100.0 P 0 QA/QC ORGANISMS

59 SR-14-BC-F-4 4 518 96.1 P Oligs. found In QA/QC

60 SR-14-BC-F-5 1 330 98.5 P Oligs. found In QA/QC

61 SR-15-BC-F-1 2 284 96.5 P Oligs. found In QA/QC

62 SR-15-BC-F-2 0 351 100.0 P 0 QA/QC ORGANISMS

63 SR-15-BC-F-3 3 515 97.1 P Oligs. found In QA/QC

64 SR-15-BC-F-4 4 494 96.0 P Oligs. found In QA/QC

65 SR-15-BC-F-5 4 495 96.0 P Oligs. found In QA/QC

66 SR-16-BC-F-1 1 344 98.5 P Oligs. found In QA/QC

P/F = Pass/Fail
EVS CONSULTANTS 20% SORTING QA/QC LOG Page 3 of 3

Environmental Services

EVS PROJECT #: 2/575-37.11 

PROJECT NAME: SHEBOYGAN RIVER 

#QA % Sorting

Sample I.D. Organisms Total Abund. Efficiency P / F Comments

67 SR-16-BC-F-2 1 363 98.6 P Oligs. found In QA/QC

68 SR-16-BC-F-3 2 214 95.3 P Oligs. found In QA/QC

69 SR-16-BC-F-4 0 313 100.0 P 0 QA/QC ORGANISMS

70 SR-16-BC-F-5 1 290 98.3 P Oligs. found In QA/QC

71 SR-17-BC-F-1 3 339 95.6 P Oligs. found In QA/QC

72 SR-17-BC-F-2 0 224 100.0 P 0 QA/QC ORGANISMS

73 SR-17-BC-F-3 3 482 96.9 P Oligs. found In QA/QC

74 SR-17-BC-F-4 2 368 97.3 P Oligs. found In QA/QC

75 SR-17-BC-F-5 1 302 98.3 P Oligs. found In QA/QC

76 SR-18-BC-F-1 0 409 100.0 P 0 QA/QC ORGANISMS

77 SR-18-BC-F-2 0 326 100.0 P 0 QA/QC ORGANISMS

78 SR-18-BC-F-3 1 298 98.3 P Oligs. found In QA/QC

79 SR-18-BC-F-4 2 327 96.9 P Oligs. found In QA/QC

80 SR-18-BC-F-5 2 441 97.7 P Oligs. found In QA/QC

81 SR-19-BC-F-1 2 318 96.9 P Oligs. found In QA/QC

82 SR-19-BC-F-2 0 335 100.0 P 0 QA/QC ORGANISMS

83 SR-19-BC-F-3 2 324 96.9 P Oligs. found In QA/QC

84 SR-19-BC-F-4 0 171 100.0 P 0 QA/QC ORGANISMS

85 SR-19-BC-F-5 2 739 98.6 P Oligs. found In QA/QC

86 SR-20-BC-F-1 0 588 100.0 P 0 QA/QC ORGANISMS

87 SR-20-BC-F-2 2 578 98.3 P Oligs. found In QA/QC

88 SR-20-BC-F-3 0 680 100.0 P 0 QA/QC ORGANISMS

89 SR-20-BC-F-4 0 405 100.0 P 0 QA/QC ORGANISMS

90 SR-20-BC-F-5 3 520 97.1 P Oligs. found In QA/QC

P/F = Pass/Fail
Benthic taxonomic results for the 1997 Sheboygan ERA

T01-1 T01-2 T01-3 T01-4 T01-5 T02-1 T02-2 T02-3 T02-4 T02-5 T03-1 T03-2 

Ablabesmyia sp. 0 0 0 0 0 0 0 0 0 0 0 0

Baetidae 0 0 0 0 0 1 0 0 0 0 0 0

Baetis sp. 0 1 0 0 0 0 0 0 0 0 0 0

Baetis tricaudatus 0 0 3 1 0 0 0 0 0 0 0 0

Caecidotea sp. 0 0 0 0 0 0 0 0 0 0 0 0

Caenis sp. 1 0 0 0 0 0 0 0 0 0 0 0

Ceratopogoninae sp. 0 0 0 0 1 0 0 0 0 0 0 0

Chironomini 0 0 0 0 0 0 0 0 0 0 0 0

Chironomus sp. 1 0 1 0 0 0 0 0 0 0 10 2

Chloroperlidae 0 0 0 0 0 0 0 0 0 0 0 0

Cladopelma sp. 0 0 0 0 0 0 0 0 0 0 0 0

Clinotanypus sp. 0 0 0 0 0 0 0 0 0 0 0 0

Coenagrionidae 0 0 0 0 0 0 0 0 0 0 0 0

Cricotopus sp. 0 0 0 0 0 0 0 0 0 0 0 0

Cryptochironomus sp. 0 0 1 1 0 0 0 0 0 0 0 1

Cryptotendipes sp. 0 0 0 0 0 0 0 0 0 0 0 0

Dicrotendipes sp. 0 0 0 0 0 0 0 0 0 0 0 0

Dineutus sp. 0 0 0 0 0 0 0 0 0 0 0 0

Dubiraphia sp. 16 25 64 43 23 0 1 5 0 1 26 2

Endochironomus sp. 0 0 0 0 0 0 0 0 0 0 0 0

Ephemeroptera 0 0 0 0 0 0 0 0 0 0 0 0

Erioptera sp. 1 0 0 0 0 0 0 0 0 0 0 0

Eukiefferiella sp. 0 0 0 0 0 1 0 0 0 0 0 0

Gammarus sp. 0 2 0 0 0 0 0 0 0 0 0 0

Glossosomatidae 0 1 0 0 0 0 0 0 0 0 0 0

Glutops sp. 0 0 0 0 0 0 0 0 0 0 0 0

Glyptotendipes sp. 0 0 0 0 0 0 0 0 0 0 0 0

Harnischia sp. 0 0 0 0 0 0 0 0 0 0 0 0

Helobdella stagnalis 0 0 0 0 0 0 0 0 0 0 0 0

Heptageniidae 0 0 0 0 0 0 0 0 0 0 0 0

Hexagenia sp. 1 0 0 0 0 0 0 0 0 0 0 0

Hirudinea 0 0 0 0 0 0 0 0 0 0 0 0

Hyalella azteca 6 0 0 0 0 0 0 0 0 0 0 0

Hydropsyche sp. 0 0 0 0 0 0 0 0 0 0 0 0

Microchironomus sp. 0 0 0 0 0 0 0 0 0 0 0 0

Microcylloepus sp. 1 0 1 1 0 0 0 0 0 0 0 0

Micropsectra sp. 0 0 0 0 0 0 0 0 0 0 0 0

Microtendipes sp. 0 0 0 0 0 0 0 0 0 0 0 0

Oecetis sp. 0 0 0 0 0 0 0 0 0 0 0 0

Ordobrevia sp. 0 0 0 1 0 0 0 0 0 0 0 0

Paralauterborniella sp. 0 0 0 0 0 0 0 0 0 0 0 0

Paramerina sp. 0 0 0 0 0 0 0 0 0 0 0 0

Paratanytarsus sp. 0 0 0 0 0 0 0 0 0 0 0 0

Paratendipes sp. 0 1 0 0 0 0 0 0 0 0 0 0

Pentaneurini 0 0 0 0 0 0 0 0 0 0 1 0

Polypedilum sp. 0 2 3 1 0 0 0 0 0 0 5 1

Procladiini 0 0 0 0 0 0 0 0 0 0 0 0

Procladius sp. 2 1 1 2 0 0 0 0 0 0 1 0

Rheotanytarsus sp. 0 1 1 0 0 0 0 0 0 0 0 0

Sialis sp. 0 0 0 0 0 0 0 0 0 0 0 0

Sphaeriidae 0 1 0 0 0 0 0 0 0 0 0 0

Stenochironomus sp. 0 0 0 0 0 0 0 0 0 0 0 0

Stictochironomus sp. 0 0 0 0 0 0 0 0 0 0 0 0

Tabanidae 0 0 0 0 0 0 0 0 0 0 0 0

Tanypus sp. 0 0 0 0 0 0 0 0 0 0 0 0

Tanytarsini 0 0 0 0 0 0 0 0 0 0 0 0

Tanytarsus sp. 0 0 0 0 0 0 0 0 0 0 0 0

Thienemannimyia gr. sp. 0 0 1 0 0 0 0 0 0 0 0 0

Tricorythodes sp. 0 0 0 0 0 0 0 0 0 0 0 0

Tubificidae 160 116 262 191 108 56 74 166 87 208 178 22

Turbellaria 0 0 0 0 0 0 0 0 0 0 0 0

Total 189 151 338 241 132 58 75 171 87 209 221 28

*see Note at end of table

Page 1 of 8
Benthic taxonomic results for the 1997 Sheboygan ERA

Ablabesmyia sp.

Baetidae

Baetis sp.

Baetis tricaudatus

Caecidotea sp.

Caenis sp.

Ceratopogoninae sp.

Chironomini

Chironomus sp.

Chloroperlidae

Cladopelma sp.

Clinotanypus sp.

Coenagrionidae

Cricotopus sp.

Cryptochironomus sp.

Cryptotendipes sp.

Dicrotendipes sp.

Dineutus sp.

Dubiraphia sp.

Endochironomus sp.

Ephemeroptera

Erioptera sp.

Eukiefferiella sp.

Gammarus sp.

Glossosomatidae

Glutops sp.

Glyptotendipes sp.

Harnischia sp.

Helobdella stagnalis

Heptageniidae

Hexagenia sp.

Hirudinea

Hyalella azteca

Hydropsyche sp.

Microchironomus sp.

Microcylloepus sp.

Micropsectra sp.

Microtendipes sp.

Oecetis sp.

Ordobrevia sp.

Paralauterborniella sp.

Paramerina sp.

Paratanytarsus sp.

Paratendipes sp.

Pentaneurini

Polypedilum sp.

Procladiini

Procladius sp.

Rheotanytarsus sp.

Sialis sp.

Sphaeriidae

Stenochironomus sp.

Stictochironomus sp.

Tabanidae

Tanypus sp.

Tanytarsini

Tanytarsus sp.

Thienemannimyia gr. sp.

Tricorythodes sp.

Tubificidae

Turbellaria

Total

*see Note at end of table

T03-3 T03-4 T03-5 T04-1 T04-2 T04-3 T04-4 T04-5 T07-1 T07-2 T07-3 T07-4 

0 0 0 0 0 0 0 0 1 1 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

1 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 1 0 0

0 0 1 0 0 0 0 0 0 0 0 0

0 0 1 0 0 0 0 0 0 0 0 0

1 0 0 0 0 0 0 0 0 0 0 0

16 19 9 1 0 0 0 0 5 3 4 5

1 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 5 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 1 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

2 2 0 0 0 0 2 3 0 2 2 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 9 3 0 1

0 0 0 0 0 0 0 0 1 0 0 0

17 23 12 2 1 0 0 0 3 6 2 1

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 1 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 35 4 1 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 1 0 0 0

0 0 0 0 0 0 1 0 0 1 1 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 3 1 0 0 0 0 0 1 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

1 1 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 1 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

1 0 1 0 0 0 0 0 0 0 1 0

12 11 8 0 0 0 0 0 3 2 1 2

0 0 0 0 0 0 0 0 0 0 0 0

1 0 4 1 3 1 9 1 15 5 5 4

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 3 1 1 0

0 0 0 5 3 0 5 8 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 1 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 2 0 0 0

236 267 182 86 100 101 83 123 2 20 12 9

0 0 0 0 0 0 0 0 0 0 0 0

289 327 219 102 107 102 100 135 82 49 30 22

Page 2 of 8
Benthic taxonomic results for the 1997 Sheboygan ERA

Ablabesmyia sp.

Baetidae

Baetis sp.

Baetis tricaudatus

Caecidotea sp.

Caenis sp.

Ceratopogoninae sp.

Chironomini

Chironomus sp.

Chloroperlidae

Cladopelma sp.

Clinotanypus sp.

Coenagrionidae

Cricotopus sp.

Cryptochironomus sp.

Cryptotendipes sp.

Dicrotendipes sp.

Dineutus sp.

Dubiraphia sp.

Endochironomus sp.

Ephemeroptera

Erioptera sp.

Eukiefferiella sp.

Gammarus sp.

Glossosomatidae

Glutops sp.

Glyptotendipes sp.

Harnischia sp.

Helobdella stagnalis

Heptageniidae

Hexagenia sp.

Hirudinea

Hyalella azteca

Hydropsyche sp.

Microchironomus sp.

Microcylloepus sp.

Micropsectra sp.

Microtendipes sp.

Oecetis sp.

Ordobrevia sp.

Paralauterborniella sp.

Paramerina sp.

Paratanytarsus sp.

Paratendipes sp.

Pentaneurini

Polypedilum sp.

Procladiini

Procladius sp.

Rheotanytarsus sp.

Sialis sp.

Sphaeriidae

Stenochironomus sp.

Stictochironomus sp.

Tabanidae

Tanypus sp.

Tanytarsini

Tanytarsus sp.

Thienemannimyia gr. sp.

Tricorythodes sp.

Tubificidae

Turbellaria

Total

*see Note at end of table

T07-5 T08-1 T08-2 T08-3 T08-4 T08-5 T09-1 T09-2 T09-3 T09-4 T09-5 T10-1 

0 0 11 8 5 1 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 3 1 1 1 0 0 1 0 0 0

0 1 1 0 0 0 0 0 1 0 0 0

0 0 0 0 0 0 2 0 2 2 0 1

0 0 0 0 0 0 2 0 0 1 2 0

0 2 3 2 1 1 1 0 0 0 1 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 1 1 0

0 2 11 1 1 3 1 0 2 0 2 4

0 0 0 0 0 0 0 0 0 5 0 0

0 4 5 4 3 0 1 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 12 24 2 10 17 4 10 13 11 12 2

0 0 1 0 0 0 0 0 0 0 0 0

0 2 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 2 3 3 2 0 0 3 7 8 7 1

0 3 1 0 2 4 17 15 12 15 18 0

0 0 0 0 0 1 0 0 0 0 0 0

0 0 2 0 0 0 0 0 0 0 0 0

1 3 2 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 1 0 0 1 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 3

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 1 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 1 0 0

0 0 0 0 1 0 0 0 0 0 0 0

0 0 0 1 0 0 1 1 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 3 3 0 2 1 0 0 5 1 1 0

0 4 0 0 1 3 11 9 4 10 12 1

0 0 0 0 0 0 0 0 0 0 2 0

0 1 0 0 0 0 22 33 51 31 30 67

0 0 0 1 1 1 0 0 0 0 0 0

0 1 0 0 0 0 0 0 0 0 0 0

1 3 4 1 0 2 0 0 1 1 0 0

0 0 1 0 0 0 0 0 0 0 1 0

0 0 0 0 0 0 0 0 0 0 0 0

0 3 4 2 0 1 0 0 5 0 1 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 1 0 0 0 0 0 0 0

0 0 0 0 0 0 2 0 0 0 0 1

0 0 0 0 0 0 0 0 0 0 0 0

0 2 2 1 2 0 0 0 0 0 0 0

3 540 535 222 385 519 310 283 262 454 430 352

0 0 0 0 0 0 0 0 1 0 0 0

5 589 617 249 419 555 374 354 367 541 520 432

Page 3 of 8
Benthic taxonomic results for the 1997 Sheboygan ERA

Ablabesmyia sp.

Baetidae

Baetis sp.

Baetis tricaudatus

Caecidotea sp.

Caenis sp.

Ceratopogoninae sp.

Chironomini

Chironomus sp.

Chloroperlidae

Cladopelma sp.

Clinotanypus sp.

Coenagrionidae

Cricotopus sp.

Cryptochironomus sp.

Cryptotendipes sp.

Dicrotendipes sp.

Dineutus sp.

Dubiraphia sp.

Endochironomus sp.

Ephemeroptera

Erioptera sp.

Eukiefferiella sp.

Gammarus sp.

Glossosomatidae

Glutops sp.

Glyptotendipes sp.

Harnischia sp.

Helobdella stagnalis

Heptageniidae

Hexagenia sp.

Hirudinea

Hyalella azteca

Hydropsyche sp.

Microchironomus sp.

Microcylloepus sp.

Micropsectra sp.

Microtendipes sp.

Oecetis sp.

Ordobrevia sp.

Paralauterborniella sp.

Paramerina sp.

Paratanytarsus sp.

Paratendipes sp.

Pentaneurini

Polypedilum sp.

Procladiini

Procladius sp.

Rheotanytarsus sp.

Sialis sp.

Sphaeriidae

Stenochironomus sp.

Stictochironomus sp.

Tabanidae

Tanypus sp.

Tanytarsini

Tanytarsus sp.

Thienemannimyia gr. sp.

Tricorythodes sp.

Tubificidae

Turbellaria

Total

*see Note at end of table

T10-2 T10-3 T10-4 T10-5 T11-1 T11-2 T11-3 T11-4 T11-5 T12-1 T12-2 T12-3 

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 1 0 0 0

0 0 0 0 0 0 0 0 0 2 0 2

1 0 0 0 0 2 0 0 0 0 0 2

1 1 0 0 6 7 4 12 6 14 29 31

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 1 0 0 0 1 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 1 0 0 0 0 0 0 0

7 4 3 2 1 3 0 1 3 1 2 2

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 1 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

1 0 1 1 7 4 0 2 4 4 2 3

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 1 0 1 0 0 1 0

1 1 0 0 7 0 0 3 1 4 8 11

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

1 3 3 1 3 1 0 5 7 65 4 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 1 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 1 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 1 0

0 0 0 0 0 0 0 0 0 0 1 1

0 0 1 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 1 0 0 0

1 1 0 1 1 2 1 6 3 0 4 3

0 0 0 0 0 0 0 0 0 0 0 0

45 45 31 34 29 21 3 22 41 17 17 8

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 1 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 2 1 0 2 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 1 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

156 247 252 215 322 215 174 277 270 264 315 318

0 0 0 0 0 0 0 0 0 0 0 0

214 302 292 254 378 259 183 329 342 371 385 381

Page 4 of 8
Benthic taxonomic results for the 1997 Sheboygan ERA

Ablabesmyia sp.

Baetidae

Baetis sp.

Baetis tricaudatus

Caecidotea sp.

Caenis sp.

Ceratopogoninae sp.

Chironomini

Chironomus sp.

Chloroperlidae

Cladopelma sp.

Clinotanypus sp.

Coenagrionidae

Cricotopus sp.

Cryptochironomus sp.

Cryptotendipes sp.

Dicrotendipes sp.

Dineutus sp.

Dubiraphia sp.

Endochironomus sp.

Ephemeroptera

Erioptera sp.

Eukiefferiella sp.

Gammarus sp.

Glossosomatidae

Glutops sp.

Glyptotendipes sp.

Harnischia sp.

Helobdella stagnalis

Heptageniidae

Hexagenia sp.

Hirudinea

Hyalella azteca

Hydropsyche sp.

Microchironomus sp.

Microcylloepus sp.

Micropsectra sp.

Microtendipes sp.

Oecetis sp.

Ordobrevia sp.

Paralauterborniella sp.

Paramerina sp.

Paratanytarsus sp.

Paratendipes sp.

Pentaneurini

Polypedilum sp.

Procladiini

Procladius sp.

Rheotanytarsus sp.

Sialis sp.

Sphaeriidae

Stenochironomus sp.

Stictochironomus sp.

Tabanidae

Tanypus sp.

Tanytarsini

Tanytarsus sp.

Thienemannimyia gr. sp.

Tricorythodes sp.

Tubificidae

Turbellaria

Total

*see Note at end of table

T12-4 T12-5 T13-1 T13-2 T13-3 T13-4 T13-5 T14-1 T14-2 T14-3 T14-4 T14-5 

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 1 0 2 0 0 0 0 0 0 0 0

0 0 0 0 1 0 0 0 0 0 0 1

23 15 26 33 15 10 16 7 6 3 16 8

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

3 4 0 0 0 0 0 0 1 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

2 2 0 0 0 0 0 1 0 0 1 1

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 1 0

1 0 0 0 0 0 0 0 0 0 0 0

5 4 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 2 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 1 0 0 0 1 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 1 0 0 0 0 0 0 0 1 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

1 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 1

0 4 0 0 0 0 0 0 1 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

13 5 4 17 12 5 5 4 3 2 4 2

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

1 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 1 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

412 320 1682 1394 918 633 666 408 331 253 495 317

0 0 0 0 0 0 0 0 0 0 0 0

461 356 1714 1446 946 648 688 421 342 259 518 330

Page 5 of 8
Benthic taxonomic results for the 1997 Sheboygan ERA

Ablabesmyia sp.

Baetidae

Baetis sp.

Baetis tricaudatus

Caecidotea sp.

Caenis sp.

Ceratopogoninae sp.

Chironomini

Chironomus sp.

Chloroperlidae

Cladopelma sp.

Clinotanypus sp.

Coenagrionidae

Cricotopus sp.

Cryptochironomus sp.

Cryptotendipes sp.

Dicrotendipes sp.

Dineutus sp.

Dubiraphia sp.

Endochironomus sp.

Ephemeroptera

Erioptera sp.

Eukiefferiella sp.

Gammarus sp.

Glossosomatidae

Glutops sp.

Glyptotendipes sp.

Harnischia sp.

Helobdella stagnalis

Heptageniidae

Hexagenia sp.

Hirudinea

Hyalella azteca

Hydropsyche sp.

Microchironomus sp.

Microcylloepus sp.

Micropsectra sp.

Microtendipes sp.

Oecetis sp.

Ordobrevia sp.

Paralauterborniella sp.

Paramerina sp.

Paratanytarsus sp.

Paratendipes sp.

Pentaneurini

Polypedilum sp.

Procladiini

Procladius sp.

Rheotanytarsus sp.

Sialis sp.

Sphaeriidae

Stenochironomus sp.

Stictochironomus sp.

Tabanidae

Tanypus sp.

Tanytarsini

Tanytarsus sp.

Thienemannimyia gr. sp.

Tricorythodes sp.

Tubificidae

Turbellaria

Total

*see Note at end of table

T15-1 T15-2 T15-3 T15-4 T15-5 T16-1 T16-2 T16-3 T16-4 T16-5 T17-1 T17-2 

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 1 1 0 0 0 0 0 0 0 0

0 0 1 0 0 0 0 0 1 0 0 0

1 7 5 8 4 20 12 17 29 16 8 3

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 1 0 0 0 0 0 0

0 0 0 0 0 0 0 1 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

1 1 1 0 0 1 0 0 0 0 1 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

1 0 0 1 0 0 0 0 0 0 0 0

0 0 0 0 0 1 1 0 1 0 0 0

1 0 0 0 0 0 0 0 0 0 0 0

0 0 0 1 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 1 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 1 0

0 0 0 0 0 0 0 0 0 0 0 0

5 3 6 5 5 10 22 8 8 8 1 1

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 1 0 0 1 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 1 1 0 1 0 0 0

0 0 0 0 0 0 0 0 0 0 1 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

275 340 501 478 486 309 326 188 273 265 327 220

0 0 0 0 0 0 0 0 0 0 0 0

284 351 515 494 495 344 363 214 313 290 339 224

Page 6 of 8
Benthic taxonomic results for the 1997 Sheboygan ERA

Ablabesmyia sp.

Baetidae

Baetis sp.

Baetis tricaudatus

Caecidotea sp.

Caenis sp.

Ceratopogoninae sp.

Chironomini

Chironomus sp.

Chloroperlidae

Cladopelma sp.

Clinotanypus sp.

Coenagrionidae

Cricotopus sp.

Cryptochironomus sp.

Cryptotendipes sp.

Dicrotendipes sp.

Dineutus sp.

Dubiraphia sp.

Endochironomus sp.

Ephemeroptera

Erioptera sp.

Eukiefferiella sp.

Gammarus sp.

Glossosomatidae

Glutops sp.

Glyptotendipes sp.

Harnischia sp.

Helobdella stagnalis

Heptageniidae

Hexagenia sp.

Hirudinea

Hyalella azteca

Hydropsyche sp.

Microchironomus sp.

Microcylloepus sp.

Micropsectra sp.

Microtendipes sp.

Oecetis sp.

Ordobrevia sp.

Paralauterborniella sp.

Paramerina sp.

Paratanytarsus sp.

Paratendipes sp.

Pentaneurini

Polypedilum sp.

Procladiini

Procladius sp.

Rheotanytarsus sp.

Sialis sp.

Sphaeriidae

Stenochironomus sp.

Stictochironomus sp.

Tabanidae

Tanypus sp.

Tanytarsini

Tanytarsus sp.

Thienemannimyia gr. sp.

Tricorythodes sp.

Tubificidae

Turbellaria

Total

*see Note at end of table

T17-3 T17-4 T17-5 T18-1 T18-2 T18-3 T18-4 T18-5 T19-1 T19-2 T19-3 T19-4 T19-5 

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 1 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 1 1 0 0 0

9 11 1 9 10 2 9 5 9 6 5 6 10

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 1 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 1 0 0

1 0 0 0 1 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

2 1 0 0 1 1 0 0 0 1 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 1 0 0 1 2 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 2 2 1 0 1

0 0 0 0 0 0 0 0 0 0 0 0 0

22 17 8 4 1 3 4 5 7 18 12 5 9

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 1 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 1 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

1 0 1 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

447 339 291 396 313 291 313 431 299 305 303 159 719

0 0 0 0 0 0 0 0 0 0 0 0 0

482 368 302 409 326 298 327 441 318 335 324 171 739

Page 7 of 8
Benthic taxonomic results for the 1997 Sheboygan ERA

Ablabesmyia sp.

Baetidae

Baetis sp.

Baetis tricaudatus

Caecidotea sp.

Caenis sp.

Ceratopogoninae sp.

Chironomini

Chironomus sp.

Chloroperlidae

Cladopelma sp.

Clinotanypus sp.

Coenagrionidae

Cricotopus sp.

Cryptochironomus sp.

Cryptotendipes sp.

Dicrotendipes sp.

Dineutus sp.

Dubiraphia sp.

Endochironomus sp.

Ephemeroptera

Erioptera sp.

Eukiefferiella sp.

Gammarus sp.

Glossosomatidae

Glutops sp.

Glyptotendipes sp.

Harnischia sp.

Helobdella stagnalis

Heptageniidae

Hexagenia sp.

Hirudinea

Hyalella azteca

Hydropsyche sp.

Microchironomus sp.

Microcylloepus sp.

Micropsectra sp.

Microtendipes sp.

Oecetis sp.

Ordobrevia sp.

Paralauterborniella sp.

Paramerina sp.

Paratanytarsus sp.

Paratendipes sp.

Pentaneurini

Polypedilum sp.

Procladiini

Procladius sp.

Rheotanytarsus sp.

Sialis sp.

Sphaeriidae

Stenochironomus sp.

Stictochironomus sp.

Tabanidae

Tanypus sp.

Tanytarsini

Tanytarsus sp.

Thienemannimyia gr. sp.

Tricorythodes sp.

Tubificidae

Turbellaria

Total

*see Note at end of table

T20-1 T20 T20-2 T20-3 T20-4 T20-5 Taxon Abun

0 0 0 0 0 27

0 0 0 0 0 1

0 0 0 0 0 1

0 0 0 0 1 6

1 0 0 0 0 10

0 0 0 0 0 6

1 0 1 1 0 21

0 0 0 0 0 17

3 4 8 5 2 620

0 0 0 0 0 1

0 1 0 1 0 5

0 0 0 0 0 5

0 0 0 0 0 1

0 0 0 0 0 5

0 0 0 0 0 83

0 0 0 0 0 5

0 0 0 0 0 31

0 0 0 0 0 1

0 0 0 1 1 439

0 0 0 0 0 1

0 0 0 0 0 2

0 0 0 0 0 1

0 0 0 0 0 1

0 0 0 0 0 3

0 0 0 0 0 1

0 0 0 0 0 1

0 0 0 0 0 80

1 0 0 0 0 133

0 0 0 0 0 1

0 0 0 0 0 3

0 0 0 0 0 12

0 0 0 0 0 2

0 0 0 0 0 6

0 0 0 0 0 4

0 0 0 0 0 105

0 0 0 0 1 10

0 0 0 0 0 1

0 0 0 0 0 4

0 0 0 0 0 2

0 0 0 0 0 3

0 0 0 0 0 1

0 0 0 0 0 4

0 0 0 0 0 2

0 0 0 0 0 2

0 0 0 0 0 23

0 0 0 0 0 141

0 0 0 0 0 2

3 3 5 5 4 897

0 0 0 0 0 5

0 0 0 0 0 4

0 0 0 0 0 16

0 0 0 0 0 2

0 0 0 0 0 1

0 0 0 0 0 21

0 0 0 0 0 30

0 0 0 0 0 4

0 0 0 0 0 4

0 0 0 0 0 2

0 0 0 0 0 9

579 570 666 392 511 29310

0 0 0 0 0 1

588 578 680 405 520 32142

Page 8 of 8
APPENDIX E

Life History of

Limnodrilus hoffmeisteri and L. cervix
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November 1998 1

APPENDIX E

LIFE HISTORY OF

LIMNODRILUS HOFFMEISTERI AND L. CERVIX

L. hoffmeisteri is a common species usually found in slow-flowing waters associated

with depositional areas (Mason 1994). Benthic algae and bacteria constitute its basic

food source (Sauter and Güde 1996). It is a highly pollution-tolerant organism often

identified in the literature as an indicator of pollution and organic enrichment (Lafont et

al. 1996; Lafont 1984; Verdonschot 1989). It is also known to be tolerant of arsenic,

copper, lead, and zinc (Lawrence and Harris 1979). L. cervix is slightly less tolerant

(Simpson et al. 1984). In general, good indicators of reduced water quality include

increased dominance of L. hoffmeisteri and Tubifex tubifex, reduction in species richness,

and increased dominance of oligochaete biomass (Finogenova 1996; Slepukhina 1984).

Rapid adaptation, ability to alter its life cycles, and ability to breed earlier than other

tubificids contribute to the numerical dominance of L. hoffmeisteri in benthic

invertebrate communities and its ability to exploit changing environmental conditions

(Kennedy 1966; Mason 1994).

Tubificids are known to alternate between sexual and asexual reproductive modes.

Asexual reproduction is accomplished chiefly through transverse fission and

parthenogenesis. In transverse fission the individual initiates powerful contractions of the

body wall causing it to fragment into pieces which regenerate new anterior and posterior

ends (Christensen 1984). Parthenogenesis (reproduction by development of an

unfertilized gamete) has been documented in L. hoffmeisteri (Poddubnaya 1984). If there

is a surplus of eggs under natural conditions, the oligochaetes often will reproduce

parthenogenetically after bisexual reproduction (Poddubnaya 1984). The ability of

L. hoffmeisteri to reach sexual maturity and breed at an earlier age gives it a distinct

advantage over other tubificid species and is reflected in its near ubiquitous presence in

organically enriched depositional environments.

Natural populations of L. hoffmeisteri usually produce a single generation each year

between spring and summer (Mason 1994). Timing of the reproductive cycle varies with

geographic location. The typical pattern in northern temperate streams is to reproduce

during May-June or August-September. Study of a broad English canal approximately

2 m deep found that young L. hoffmeisteri hatched in spring and the bulk of the

population was in the immature stage in early summer. Sexual maturity of L. hoffmeisteri

occurs between the first and second years of life depending on the physiochemical

conditions present (Poddubnaya 1980). Most individuals die after breeding, but some are

capable of breeding a second time in the same season (Kennedy 1966). Tubificid worms

can potentially live for several years (Pasteris et al. 1996).
C:\SHEBOYGAN PDF\E-LIFEHI\APPEND-E.DOC

November 1998 2

In a number of studies L. hoffmeisteri is the dominant taxon present, occurring in

densities ranging from 503/m3 to 2167/m3 in a river in Indiana to 46,600/m2 in a river

delta in St. Petersburg, Russia; accounting for up to 95 percent of the tubificid population

(Block et al. 1982; Finogenova 1996). L. hoffmeisteri is very sensitive to population

density and maturation time is density-dependent. Moderate population densities are

reported as < 20,000/m2 and densities > 25,000/m2 are detrimental to survival (Mason

1994). Increased density also negatively affects the percentage of a population that is able

to reach maturity (Adreani et al. 1984).

Review of the literature indicates cases where mature L. hoffmeisteri are not observed at

various times of the year (Block et al. 1982; Kennedy 1966; Steinlechner 1987). Tubificid

populations composed mainly of immature specimens have been found to occur during the

summer months in England and in the Little Calumet River in Indiana (Block et al. 1982;

Kennedy 1966). Routinely, up to 80 percent of the collected oligochaete material may

contain immature specimens which cannot be identified to species (Adreani et al. 1984;

Steinlechner 1987).

Water temperature has been shown to have a direct effect on density and health of

oligochaete populations in “unproductive” habitats containing few nutrients (Mason

1994). In situ measurements of growth vs. temperature found that L. hoffmeisteri and

T. tubifex only grew within a very narrow temperature range between 10 and 13°C. A

0.3°C change in temperature from 9.7 to 10.0 appeared to initiate growth (Reynoldson

1987). Other studies indicate that temperatures below 8–12°C interrupt reproductive

activity (Poddubnaya 1959; Block et al. 1982). Differences in egg production between

L. hoffmeisteri and T. tubifex have been documented with L. hoffmeisteri exhibiting

higher egg production levels at higher temperatures of 20–25°C and T. tubifex at lower

temperatures of 8°C (Reynoldson 1987; Aston 1973).

The conventional assumption is that oligochaetes as a group prefer or require

environments characterized by fine-grained substrates. At the family level this is

generally true for tubificids, however, at the species level there appears to be significant

variation in the range of exploitable substrate types (Sauter and Güde 1996). Numerous

studies indicate that the growth and distribution of L. hoffmeisteri are relatively

insensitive to sediment type (Reynoldson 1987). L. hoffmeisteri can inhabit substrates

ranging from 15 to 90 percent clay and silt with abundance peaks occurring at 20 to

30 percent and 50 to 60 percent clay and silt (Sauter and Güde 1996). Results from the

Main Channel, Hudson River study indicate that L. hoffmeisteri is able to inhabit a wide

range of substrate types, reaching maximum abundance in high-organic content, silty-sand

areas (Simpson et al. 1984).
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November 1998 3

REFERENCES

Adreani, L., C. Bonacina, G. Bonomi, and C. Monti. 1984. Cohort cultures of

Psammoryctides barbatus (Grube) and Spirosperma ferox Eisen: a tool for better

understanding of demographic strategies in Tubificidae. Hydrobiologia. 115:113-

119.

Aston, R.J. 1973. Field and experimental studies on the effects of a power station

effluent on Tubificidae (Oligochaeta, Annelida). Hydrocol. Bulletin. 42:242-255.

Block, E.M., G. Moreno, and C.J. Goodnight. 1982. Observations on the life history of

Limnodrilus hoffmeisteri (Annelida, Tubificidae) from the Little Calumet River in

temperate North America. Elsevier Biomedical Press. International Journal of

Invertebrate Reproduction.

Christensen, B. 1984. Asexual propagation and reproductive strategies in aquatic

Oligochaeta. Hydrobiologia. 115:91-95.

Finogenova, N.P. 1996. Oligochaete communities at the mouth of the Neva and their

relationship to anthropogenic impact. Hydrobiologia. 334:185-191.

Kennedy, C.R. 1966. The life history of Limnodrilus hoffmeisteri Clap. (Oligochaeta:

Tubificidae) and its adaptive significance. Oikos. 17(2):158-168.

Lafont, M. 1984. Oligochaete communities as biological descriptors of pollution in the

fine sediments of rivers. Hydrobiologia. 115:127-129.

Lafont, M., J.C. Camus, and A. Rosso. 1996. Superficial and hyporheic oligochaete

communities as indicators of pollution and water exchange in the River Moselle,

France. Hydrobiologia. 334:147-155.

Lawrence, T.M. and T.L. Harris. 1979. A quantitiative method for ranking the water

quality tolerances of benthic species. Hydrobiologia. 67(3):193-196.

Mason, W.T. Jr. 1994. A review of life histories and culture methods for five common

species of Oligochaeta (Annelida). World Aquaculture. 25(1):69-75.

Pasteris, A., G. Bonomi, and C. Bonacina. 1996. Age, stage and size structure as

population state variable for Tubifex tubifex (Oligochaeta, Tubificidae).

Hydrobiologia. 334:125-132.

Poddubnaya, T.L. 1959. Concerning the dynamics of the Tubificidae populations

(Oligochaeta, Tubificidae) in Tybinsk Reservoir, Trudy Inst. Biol. Vodokhran, 2.

102.
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November 1998 4

Poddubnaya, T.L. 1980. In: Aquatic Oligochaete Biology. Brinkhurst, R.O., Cook D.G.,

eds. Plenum Press, New York. P. 175-184.

Poddubnaya, T.L. 1984. Parthenogenesis in Tubificidae. Hydrobiologia. 115:97-99.

Reynoldson, T.B. 1987. The role of environmental factors in the ecology of tubificid

oligochaetes – an experimental study. Holarctic Ecol. 10:241-248.

Sauter, G. and H. Güde. 1996. Influence of grain size on the distribution of tubificid

oligochaete species. Hydrobiologia. 334:97-101.

Simpson, K.W., R.W. Bode, J.P. Fagnani, and D.M. DeNicola. 1984. The freshwater

macrobenthos of the main channel, Hudson River, Part B: Biology, taxonomy and

distribution of resident macrobenthic species. Wadsworth Center for Laboratories

and Research. New York State Department of Health.

Slepukhina, T.D. 1984. Comparison of different methods of water quality evaluation by

means of oligochaetes. Hydrobiologia. 115:183-186.

Steinlechner, R. 1987. Identification of immature tubificids (Oligochaeta) of Lake

Constance and its influence on the evaluation of species distribution.

Hydrobiologia. 155:57-63.

Verdonschot, P.F.M. 1989. The role of oligochaetes in the management of waters.

Hydrobiologia. 180:213-227.
APPENDIX F

Parameterization of the Time-Dependent

Food Web Bioaccumulation Model
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APPENDIX F

PARAMETERIZATION OF THE TIME-DEPENDENT

FOOD WEB BIOACCUMULATION MODEL

This appendix details the data sources used in the parameterization of the food web

bioaccumulation model. Model parameters are divided into three major categories:

chemical properties, physical attributes of environmental media, and trophic (food web)

interactions. Additional information concerning model structure is available in Gobas

(1993), Gobas et al. (1995), and Morrison et al. (1996, 1997).

CHEMICAL PROPERTIES AND CONCENTRATIONS

Chemical properties were obtained from Gobas (pers. comm. 1998), who has

accumulated a database of chemical data for use in environmental modeling. The values

used are presented in Table F-1.

Table F-1. Chemical properties of PCB congeners

PCB CONGENER

MOLECULAR

WEIGHT

(g/mol)

LOG(OCTANOL-WATER PARTITION

COEFFICIENT)

(logKow)

HENRY LAW CONSTANT

(Pa m3/mol)

PCB 066 292 6.2 40

PCB 077 292 6.36 1.72

PCB 105 326.5 6.65 20

PCB 118 326.5 6.74 20

PCB 126 326.5 6.89 19

PCB 156 361 7.18 20

High resolution congener-specific PCB concentrations were measured in sediment

samples collected from the Sheboygan River for the 1997 ERA. Since bioaccumulation

in fish represents time-weighted exposure to a range of sediments, input to the model was

based on mean concentrations of all the sediment composites (numbering three to five) in

each sampling segment (Table F-2).
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November 1998 2

Table F-2. PCB congener concentrations

in sediment collected for the 1997 ERA

SAMPLING

SEGMENT

PCB 066

(:g/kg)

PCB 077

(:g/kg)

PCB 105

(:g/kg)

PCB 118

(:g/kg)

PCB 126

(:g/kg)

PCB 156

(:g/kg)

Segment 1 0.19 0.0163 0.26 0.593 0.0027 0.0572

Segment 2 310 28.1 146.7 295 0.693 16.3

Segment 3 89.7 9.05 40.7 83 0.249 9.01

Segment 5 50 5.95 23.8 43 0.176 4.35

PHYSICAL ATTRIBUTES OF ENVIRONMENTAL MEDIA

Total organic carbon (TOC) content in sediment was estimated by averaging the organic

carbon content in all samples throughout the river. The TOC results from the WDNR

food chain study and the 1997 ERA sampling program were averaged, yielding a mean

value of 4%. While separate TOC levels for each segment could be used in the model, the

sampling results exhibited very little variability; therefore the mean was used.

Values for air temperature, water temperature, total suspended solids, and organic carbon

content of particulates were available on a monthly basis, and were therefore incorporated

into the model in a time-dependent fashion (Table F-3; WDNR 1996). Since the model

uses a bioenergetics equation which is dependent on water temperature, it accounts

explicitly for the reduced feeding of fish during the winter months.

TROPHIC INTERACTIONS

To develop the food-web bioaccumulation model for smallmouth bass, estimates of

feeding behavior are required for each trophic level. Ideally, prey preferences are

determined using site-specific stomach contents analyses. In the WDNR food chain study,

stomachs from each fish type were preserved at each sampling location. Unfortunately,

most of the contents were unrecognizable, and detailed identification of contents has not

been attempted. However, some small crayfish were observed in adult smallmouth bass

stomachs.
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November 1998 3

Table F-3. Physical attributes of environmental media

MONTH

AIR

TEMPERATURE

(

è C)

WATER

TEMPERATURE

(

è C)

TOTAL SUSPENDED

SOLIDS

(kg/L)

PARTICULATE

ORGANIC CARBON

(%)

January -7 2 5.31e-06 11.25

February -2 4 1.00e-05 8.08

March 2 7 2.65e-05 7.03

April 6 10 2.83e-05 7.79

May 10 15 4.40e-05 5.94

June 15 20 3.44e-05 10.27

July 21 24 1.72e-05 11.03

August 17 20 2.69e-05 10.58

September 13 15 2.41e-05 8.62

October 8 10 1.67e-05 11.08

November 3 7 1.81e-05 8.60

December -2 4 6.65e-06 9.08

Source: WDNR (1996)

Since the smallmouth bass is an upper-trophic-level consumer, the food web is fairly

complex. In order to simplify the food web for modeling purposes, biota occupying

similar ecological niches were grouped together. For example, benthic fish, represented in

the Sheboygan River by white sucker, redhorse, and carp, were considered together since

they have similar dietary composition and sizes. The estimated lipid contents of primary

producers and benthos are presented in Table F-4. The dietary composition for each type

of predator considered in the food web model is summarized in the following section and

Table F-5.

Table F-4. Estimated lipid contents of primary producers and benthos

ORGANISM

ESTIMATED

LIPID

CONTENT (%) SOURCE

Phytoplankton 1.2 Morrison et al. 1996

(Western Lake Erie phytoplankton)

Microcrustacea 1.0 Morrison et al. 1997 (Western Lake Erie zooplankton)

Bivalves 1.3 Morrison et al. 1997

(Western Lake Erie zebra mussels)

Aquatic insect

larvae

4.5 WDNR 1996 (food chain data; Sheboygan River larval invertebrates,

including Tricoptera, Diptera, Ephemeroptera, and Chironomid larvae;

mean of segments used)
Table F-5. Physiological data and dietary assignments used to parameterize the food chain bioaccumulation model

Diet compositioni as percent of volume (age class of prey in parentheses)

Organism

Age 

Class 

(year)

Mean 

Weight 

(grams)

Whole 

Organism 

Lipid (%)

Plant Matter j 

(%)

MicroCrustaceans (%)Sediment 

(%)

Bivalves

(%)

Aquatic 

Insects 

(%)

Centrarchid 

Fish (%)

Benthic 

Fish 

(%)

Crayfish 

(%)

Young-ofYear Fish (%)Cypriniform 

Minnows (%)

Smallmouth Bass 0+ 0.85a

3.9e

- 10 - - 50 - - - 40 (0+) -

1+ 28.4a

2.9f

- - - - 10 - - 45 (0+) 20 (0+) 25 (0+)

2+ 54.0a

2.9f

- - - - 10 - - 50 (0+) 20 (0+) 20 (0+)

3+ 95.1a

2.9f

- - - - 10 - - 55 (0+) 20 (0+) 25 (1+)

4+ 255.6a

3.1g

- - - - 10 5 (1+) 20 (1+) 55 (1+) - 10 (1+)

5+ 505.5a

3.1g

- - - - 10 5 (1+) 20 (1+) 55 (1+) - 10 (1+)

6+ 640.4a

3.1g

- - - - 10 5 (1+) 20 (1+) 55 (1+) - 10 (1+)

7+ 773.9a

3.1g

- - - - 10 5 (2+) 20 (2+) 55 (2+) - 10 (2+)

8+ 945.7a

3.1g

- - - - 10 5 (2+) 20 (2+) 55 (2+) - 10 (2+)

Benthic Fish 0+ 4.0b

2.8e

- 40 - - 60 - - - - -

1+ 13b

3.6g

15 10 5 20 50 - - - - -

2+ 81b

3.6g

15 10 5 20 50 - - - - -

3+ 208b

3.6g

15 10 5 20 50 - - - - -

4+ 413b

3.6g

15 10 5 20 50 - - - - -

5+ 644b

3.6g

15 10 5 20 50 - - - - -

Crayfish 0+ 0.5c

1.3g

60 - 15 10 15 - - - - -

1+ 2.0c

1.3g

60 - 15 10 15 - - - - -

2+ 20c

1.3g

60 - 15 10 15 - - - - -

Young-of-Year Fish 0+ 8.5d

0.034e

- 40 - - 60 - - - - -

Cypriniform Minnow 0+ 8.5d

4.7h

20 30 - - 50 - - - - -

1+ 28.0d

4.7h

20 30 - - 50 - - - - -

2+ 33.6d

4.7h

20 30 - - 50 - - - - -

a

 Smallmouth bass growth rates based on mean of Lake Erie and Lake Huron studies (Scott and Crossman 1973).

b

 Benthic fish growth rates based on study of silver redhorse (Moxostoma anisurum; Meyer 1962; cited in Scott and Crossman 1973).

c

 Best professional judgment.

d

 Growth rates based on Lake Simcoe, Ontario growth of young-of-the-year shiner (Notropis atherinoides; McCrimmon 1954; cited in Scott and Crossman 1973).

e

 Mean lipid content (young-of-year fish) from four sampling segments from the WDNR food chain study. Species collected included smallmouth bass and white sucker.

f

 Mean lipid content (juvenile smallmouth bass) from four sampling segments from the 1997 ERA.

g

 Mean lipid content (adults) from four sampling segments from the WDNR food chain study. Species collected included smallmouth bass, longnose dace and white sucker (benthic), and crayfish.

h

 Lipid content of emerald shiner (Notropis atherinoides ) collected in Western Lake Erie (Morrison et al. 1997).

i

 Dietry composition estimated using Scott and Crossman (1973), Morrison et al. (1997), and Lyons pers. comm. (1997).

j

 Includes phytoplankton, algae, plant detritus, etc.
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November 1998 5

Smallmouth Bass

The smallmouth bass (Micropterus dolomieui) is a piscivorous predator that feeds on

insects, crayfish, and various fish (Scott and Crossman 1973). As young bass grow, their

diet progresses from plankton, to immature aquatic insects, to crayfish and fishes (Scott

and Crossman 1973). Smallmouth bass are very opportunistic feeders; therefore no single

species may be identified as preferred fish prey. In the Sheboygan River, both

centrarchids (sunfish and rock bass) and cypriniformes (white sucker, minnow, and other

species) have been noted as smallmouth bass prey items.

Smallmouth bass actively feed during only about half of the year; during the winter they

feed very little and are relatively inactive below a water temperature of approximately

15°C. Below 10°C, the smallmouth bass will scarcely feed at all (Lyons pers. comm.

1997). For adult smallmouth bass in most habitats, crayfish make up the majority of the

diet, followed in order of importance by fish and aquatic and terrestrial insects (Scott and

Crossman 1973). However, since the smallmouth bass sampled for the 1997 ERA

represent juveniles in the size range of 11–20 cm, it is important to assess the age- and

size-specific prey composition for this species. Lyons (pers. comm. 1997), indicates that

smallmouth bass in this size range will still feed preferentially on crayfish, provided that

the appropriate size range of crayfish is available. Dense populations of crayfish would

likely support a range of size-classes, from which appropriate prey may be selected. For

smallmouth bass in the 11–20 cm size range, the crayfish selected would be about 1 cm

long or smaller (Lyons pers. comm. 1997).

Fish are also a significant dietary component for juvenile smallmouth bass. Because

smallmouth bass are non-specialized piscivores, a major determinant of dietary

composition is the abundance of fish of a suitable size. Because juvenile smallmouth

bass are relatively small, they would consume suitably sized young-of-year fish such as

suckers, sunfish, and small minnows (Lyons pers. comm. 1997). Because minnows are

small throughout their life-cycle, they would likely be consumed more over the course of

the year than other species, which would grow to sizes too large to be consumed by

juvenile smallmouth bass. Representative minnow species include common shiner

(Notropis cornutus), sand shiner (Notropis stramineus), blackside darter (Percina

maculata), and hornyhead chub (Nacomis biguttatus).

During brief periods, insects (e.g., mayfly larvae) may be a significant component of the

smallmouth bass diet, but overall they would amount to only about 5–10% of the dietary

intake (Lyons pers. comm. 1997). Insect prey is likely most important in the months of

April and May, when young-of-year fish are not yet available. Small fish would compose

a larger proportion of the juvenile smallmouth bass diet in June and July. As these fish

grow beyond the size preferred by smallmouth bass, crayfish tend to dominate the diet.
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November 1998 6

Benthic Fish

Benthic fish are bottom feeding fish that live and feed in direct contact with the sediments.

Redhorses (Moxostoma sp.) feed exclusively on invertebrates, particularly immature

insects and small molluscs and crustaceans, that they suck out of the bottom sediments.

White suckers (Catostomus commersoni) also consume primarily invertebrate prey such

as chironomids, cladocerans, and molluscs. Carp (Cyprinus carpio) are omnivorous and

consume a variety of plant and animal tissues including aquatic insects, crustaceans,

annelids and molluscs as well as seeds, aquatic plants, and algae (Scott and Crossman

1973).

Cypriniform Minnows

Cypriniform minnows are short-lived, small fish which may be consumed by centrarchids

because of their suitable size range. In general, minnows are omnivorous, with a

preference toward consumption of animal food. The common shiner (Notropis cornutus),

which is a known prey item of smallmouth bass, consumes aquatic insects (adults and

larvae), as well as algae, aquatic plants, and other microorganisms. The sand shiner

(Notropis stramineus) consumes aquatic and terrestrial insects and bottom ooze diatoms.

Blackside darters (Percina maculata) consume mayfly and midge larvae, corixid nymphs,

copepods, and, occasionally, small fish. Hornyhead chub (Nacomis biguttatus) consume

both plant and animal tissues, with herbivorous material more important in the diet of

young fish, which also consume microcrustaceans (e.g., cladocerans) and aquatic insect

larvae such as chironomids. Larger hornyhead chub consume more aquatic insect larvae,

annelids, crayfish, and small fish (Scott and Crossman 1973).

Young-of-Year Fish

In their early life stages, many fish species in the Sheboygan river have not yet developed

specialized feeding preferences, and therefore may be represented as a single broad class

of fish with similar diets. Young-of-year fish feed mainly on zooplankton and benthic

invertebrates (e.g., insect larvae and microcrustaceans).

Crayfish

Crayfish are macrocrustaceans that are omnivorous, with a tendency to consume more

plant than animal material. Crayfish ingest mainly the primary resources at the bottom of

the food web, such as detritus and sediment, algae, and aquatic macrophytes. While plant

matter and filamentous algae are consumed by all age classes of crayfish, predation of

crayfish on a diversity of animal prey including caddisfly larvae, small bivalves, small

crayfish, and occasionally small fish, has been well documented (Zaranko et al. 1997).
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November 1998 7

REFERENCES

Gobas, F.A.P.C. 1993. A model for predicting the bioaccumulation of hydrophobic

organic chemicals in aquatic food webs: application to Lake Ontario. Ecological

modeling.69:1-17.

Gobas, F.A.P.C. 1998. Personal communication (electronic mail and telephone

discussion Gary Lawrence in January 1998 concerning chemical properties of

PCB congeners). School of Resource and Environmental Management, Simon

Fraser University, Burnaby, British Columbia, Canada.

Gobas, F.A.P.C., M.N. Z’Graggen, and X. Zhang. 1995. Time response of the Lake

Ontario ecosystem to virtual elimination of PCBs. Environ. Sci. Technol.

29(8):2038-2046.

Lyons, J. 1997. Personal communication (telephone conversation with Jim Starkes in

December 1997, regarding dietary composition of smallmouth bass in the

Sheboygan River). Fisheries Biologist, Wisconsin Department of Natural

Resources, Monona, WI.

McCrimmon, H.R., and O.E. Devitt. 1954. Winter studies on the burbot, Lota lota

lacustris, of Lake Simcoe, Ontario. Can. Fish Cult. 16:34-41.

Meyer, W.H. 1962. Life history of three species of redhorse (Moxostoma) in the

Des Moines River, Iowa. Trans. Amer. Fish. Soc. 91(4):412-419.

Morrison, H.A., F.A.P.C. Gobas, R. Lazar, and G.D. Haffner. 1996. Development and

verification of a bioaccumulation model for organic contaminants in benthic

invertebrates. Environ. Sci. Technol. 30:3377-3384.

Morrison, H.A., F.A.P.C. Gobas, R. Lazar, D.M. Whittle, and G.D. Haffner. 1997.

Development and verification of a benthic/pelagic food web bioaccumulation

model for PCB congeners in Western Lake Erie. Environ. Sci. Technol. 31:3267-

3273.

Scott, W.B. and E.J. Crossman. 1973. Freshwater fishes of Canada. Department of

Fisheries and Oceans, Fisheries Research Board of Canada, Ottawa. Bulletin 184.

966 pp.

WDNR. 1996. Data collected for the Sheboygan River food chain and contaminant

assessment. Wisconsin Department of Natural Resources, Southeast District

Water Resources Management Section.

Zaranko, D.T., R.W. Griffiths, and N.K. Kaushik. 1997. Biomagnification of

polychlorinated biphenyls through a river food web. Environ. Toxicol. Chem.

16(7):1463-1471.
APPENDIX G

Impacts on Lake Michigan Salmonids
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APPENDIX G

IMPACTS ON LAKE MICHIGAN SALMONIDS

The Sheboygan River has annual runs of chinook salmon, coho salmon, and steelhead

trout; the juveniles of these species also rear in the river before outmigrating to Lake

Michigan. Although salmonids are not permanent residents of the Sheboygan River, the

river is a significant contributor of polychlorinated biphenyls (PCBs) to Lake Michigan

and hence provides a level of exposure that may impact the salmonid runs. This section

presents an evaluation of the potential impacts to Lake Michigan salmonids. It includes

an examination of the pertinent literature regarding bioaccumulation of PCBs in

salmonids, biological effects in salmonids observed in the field, and PCB loading to Lake

Michigan,

PCBS IN LAKE MICHIGAN SALMONIDS

Over the past 25 years, several species of salmonids in Lake Michigan have been

susceptible to accumulating high concentrations of PCBs and other organochlorine

substances in their tissues. The indigenous lake trout and the introduced Pacific

salmonids (coho and chinook salmon and steelhead trout) have been studied the most.

Since the implementation of PCB restrictions in the 1970s, Lake Michigan salmonids

have demonstrated marked decreases in concentrations of total PCBs. Concentrations

appear to have peaked in the early to mid-1970s, generally followed by declines to about

the mid-1980s. Concentrations since the mid-1980s appear to have leveled off, with what

may be slight increases in coho and chinook salmon (DeVault et al. 1996; Stow et

al. 1994) (Table G-1). The highest concentrations have been observed in lake trout in

which mean tissue burdens as high as 22.9 mg/kg were observed in 1974. Concentrations

have since leveled off to between 2 and 3 mg/kg (DeVault et al. 1996). Chinook salmon

appear to have the next highest burdens with concentrations leveling off around 1 to

2 mg/kg followed by coho salmon where concentrations have been below 1 mg/kg

since 1984 (Stow et al. 1994). Although mean concentrations were not presented, Stow et

al. (1995) reported that tissue burdens and trends in steelhead and brown trout were

similar to those in coho salmon.

The level of PCB accumulation observed in Lake Michigan salmonids is also consistent

with the life history, behavior, and ecology of each species. As reported, the highest tissue

burdens of PCBs have been observed in adult lake trout followed by chinook salmon and

coho salmon. Tissue burdens in steelhead trout have been similar to coho salmon. On the

basis of exposure periods, this would be expected because the lake trout is a long-lived

species living in excess of 10 years in Lake Michigan. The species is not
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November 1998 2

Table G-1. Mean tissue concentrations (mg/kg ww) of total PCBs

in Lake Michigan salmonids

YEAR LAKE TROUTa COHO SALMONb CHINOOK SALMONb

1972 12.9 na na

1973 18.9 na na

1974 22.9 5.3 11.7

1975 22.3 3.5 na

1976 18.7 14.3 7.4

1977 11.6 na na

1978 8.2 5.8 8.8

1979 8.8 3.1 6.1

1980 9.9 1.7 4.5

1981 6.5 1.3 3.1

1982 5.6 1.9 4.8

1983 na 1.5 2.1

1984 4.5 0.7 2.2

1985 na 0.9 1.3

1986 2.6 0.9 2.3

1987 na na 1.6

1988 3.2 0.7 2.1

1989 na 0.8 1.2

1990 2.7 na na

1991 na na na

1992 3.5 na na

NOTE: na - not available

a DeVault et al. 1996

b Stow et al. 1994

anadromous, rather it spawns and rears in the lake. The chinook salmon has the next

longest life-span, spending 3 to 4 years in Lake Michigan, while the coho salmon and

steelhead trout spend 2 or 3 years in the lake before their spawning migrations to natal

streams. None of the anadromous species feed substantially during their spawning runs

and therefore would not be expected to accumulate substantial PCB burdens as adults in

the river. In addition to lake residence, chinook and coho salmon and steelhead trout rear

in natal streams as juveniles for up to one year before outmigrating to the lake.

Studies have also examined tissue burdens in forage species such as alewife, which are

considered the preferred prey of adult salmon and steelhead trout in Lake Michigan. Stow

et al. (1995) evaluated the trends in PCB tissue burdens in alewife and bloater chub,

another forage species, from the 1970s to early 1990s and found similar decreases in total
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November 1998 3

PCBs in both species over the period. Rapid decreases from the early 1970s to

mid-1980s occurred, followed by stabilizing levels after 1985. The similarity between

alewife and predator PCB concentration trends is consistent with the concept that the food

web is the primary route of PCBs into the upper trophic levels. Several other studies

(Rasmussen et al. 1990; Oliver and Niimi 1988) have illustrated the importance of the

food web in determining PCB concentrations in lake trout in Lake Ontario and other large

lake systems.

Stow et al. (1995) surmised that the observed trends in PCB tissue burdens are consistent

with ecological changes in the forage base that have occurred in Lake Michigan. During

the 1980s, alewife underwent a large decrease in number and biomass in Lake Michigan

largely in response to predation by rapidly growing chinook and coho salmon populations

(chinook and coho salmon were first introduced to Lake Michigan in the mid-1960s).

During the same period, the bloater chub population increased substantially. This

decrease in the primary forage base for salmonids coincided with decreases in the growth

of chinook and coho salmon. A decrease in growth as a consequence of lower prey

availability could result in a decreased-growth dilution effect and be responsible for the

slight increases recently observed in adult salmonid tissue burdens. Similarly, a shift in

consumption from alewife to bloater chub, which may have occurred as a result of

changing prey availability, should not result in a marked change in predator PCB

concentrations because of the similar concentrations found in alewife and bloater chub.

However, the energetics of forage and consumption are probably different for the two prey

species because alewife tend to occupy warmer surface waters while bloater chub are

usually found in deeper, cooler waters. A shift in prey may increase the energy

requirements of salmonids as they forage in cooler, deeper water and hence they may

experience reduced growth.

BIOLOGICAL EFFECTS

As reported, several studies have examined the potential biological effects of high tissue

burdens of PCBs in salmonids of the Great Lakes. Leatherland (1993) reviewed the

available evidence of reproductive and developmental problems that have been identified

in populations and stocks of Great Lake salmonids. Biological effects that have been

observed include thyroid enlargement, lowered egg thyroid hormone content, high

prevalence of premature sexual maturation in males, loss of secondary sexual

characteristics, reduced plasma gonadotropin, change in gonadal steroid hormone content,

low egg fertility, and high embryo mortality and deformity. The evidence for an etiology

based on the presence of toxic compounds is not as clear as that for fish-eating birds and

mammals; however, when taken together, there is strong evidence for environmentallyrelated impairment of endocrine and reproductive function in salmonids of the Great

Lakes.
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November 1998 4

The strongest correlations between PCBs and biological effects have been found in lake

trout. Mac et al. (1993) measured total PCBs and individual PCB congeners in eggs from

lake trout captured from Lakes Michigan, Huron, Ontario, and Superior and evaluated

several biological endpoints including hatching success, swim-up mortality, fry survival,

and fry abnormalities. Results of correlation analysis between the biological endpoints

and concentrations of PCBs in the eggs demonstrated a significant relationship between

hatching success and a number of indices of PCB contamination. The strongest

correlation was between total egg mortality and total PCB concentrations in the eggs

(r=0.701, p=0.0002). Nearly all of the individual PCB congeners showed strong

correlation with total egg mortality as well, but not as strong as total PCBs. Total PCB

concentrations in eggs were negatively correlated with the percent of normal fry hatching

(r=-0.686, p=0.0002). Total PCBs in eggs also correlated significantly with age and

length of adult females, early egg mortality, and the incidence of the biological

abnormality of fry called blue sac. In addition, there was a significant negative correlation

between PCB concentrations in adult tissues and the percent normal fry hatch (r=-0.76,

p=-0.01). The study did not report any threshold tissue concentrations at which

reproductive impacts were significantly higher. No correlation was found between PCB

concentrations and fry mortality.

Similar results were obtained by Ankley et al. (1991) who compared hatching success and

fry survival of eggs of Lake Michigan chinook salmon to total PCBs and dioxin

equivalents. In 10 groups of eggs, a significant correlation between the percent hatch of

eggs and the total PCB concentrations in the eggs was found, but no significant relation

was found to dioxin equivalents (calculated using mammalian TEFs) or between fry

survival and any measure of PCB contamination.

Contrasting results were found by Williams and Giesy (1992) who examined the relation

between measures of reproductive success of Lake Michigan chinook salmon eggs and

PCB residues. In 20 groups of eggs, no significant correlation was found between PCB

contamination and any of six measures of reproductive success. This study measured

PCB contamination in eggs as a concentration or as dioxin equivalents calculated using

several different sets of potency factors (Safe 1990; Tillitt et al. 1991; Newsted 1991); the

Newsted (1991) TEFs were derived based on Ah-mediated responses in fish.

Although data were not sufficient to determine threshold concentrations where

reproductive effects would be expected, the studies imply that lake trout are more

sensitive than chinook salmon. The average concentration of total PCBs in salmon eggs

was higher (7.02 μg/g, standard deviation 2.34) than that in lake trout eggs (1.32 μg/g,

standard deviation 1.53) (Mac et al. 1993). The range of biological responses found in

chinook salmon was also much lower than for lake trout; only 3 of 20 groups of chinook
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November 1998 5

salmon eggs suffered hatching mortality greater than 10% compared with 14 of 23 groups

of lake trout eggs (Mac et al. 1993; Williams and Giesy 1992).

PCB LOADING TO LAKE MICHIGAN

Robertson (1996) used frequency-volume analyses to estimate the PCB loads of major

tributaries to Lake Michigan and found that a small number of rivers contribute the great

majority of PCBs to the lake. Eighty percent of the load of total PCBs to Lake Michigan

was calculated to come from two basins, the Fox and Kalamazoo rivers. Six additional

basins, including the Sheboygan River, were calculated to contribute an additional 18.5%

of the load. Robertson (1996) estimated that the Sheboygan River contributed an average

of 1.92% of the total PCB load to Lake Michigan for the 16-year period from 1975 to

1990. Using data from 1980 to 1983 presented in Marti and Armstrong (1990), PCB

loading from the Sheboygan River during that period would have been approximately

21 kg/year. Hall (pers. comm. 1998) estimated that the Sheboygan River contributed

4.4% of the total PCB load to Lake Michigan in 1994 (24 kg/year). The mean water

concentration for the Sheboygan River was 83 ng/L (55–105 ng/L), which was higher

than any of the other tributaries including the Fox, 65 ng/L, and Kalamazoo, 31 ng/L

(Hall pers. comm. 1998).

CONTRIBUTION OF PCBS FROM THE SHEBOYGAN RIVER TO LAKE

MICHIGAN SALMONIDS

A study by Eggold et al. (1996) found that juvenile salmon planted in the Sheboygan

River accumulate significant levels of PCBs in their tissues before outmigrating to Lake

Michigan. To determine the relative contribution of PCB contamination in the Sheboygan

River to the tissue burdens of salmonids the study evaluated juvenile salmonids during

their residence in the Sheboygan River and again when the fish returned as adults. The

study released juvenile steelhead trout and coho salmon in the river and analyzed tissue

samples for PCBs on a monthly basis until outmigration. Upon returning after one to

three years of residence in Lake Michigan, adult tissues were analyzed for PCBs. Juvenile

steelhead trout residing in the river were found to accumulate a mean of over 1 mg/kg

(whole body, wet weight) after one month and 6.1 mg/kg after 8 months just prior to

outmigration. Juvenile coho salmon showed similar trends with a mean of 0.84 mg/kg

after 1 month and up to 4.1 mg/kg prior to outmigration. Returning steelhead trout and

coho salmon adults that had spent two or three years residing in Lake Michigan had mean

tissue burdens of 0.62 and 0.73 mg/kg (skin-on fillets), respectively. No significant

differences were observed between Sheboygan River adults and fish tissues from reference

rivers (Root and Pigeon Rivers). Considering PCB loading data previously discussed,

PCBs from the Sheboygan River would have been accumulated in the fish from the

reference rivers during their residence in Lake Michigan.
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November 1998 6

The results of Eggold et al. (1996) indicated that juveniles stocked in the Sheboygan

River during the fall months of 1980, 1991, and 1992 accumulated PCBs rapidly over the

winter before outmigration in the spring. While PCB accumulation during stream

residence did not contribute substantially to PCB concentrations in adults, the total body

burdens of PCBs from juvenile steelhead trout prior to outmigration averaged 70.5 μg per

whole fish (6.1 mg/kg). An adult steelhead, which weighed 5.2 kg after three years

residence in Lake Michigan, contained 6,216 μg of PCBs and a concentration of

1.2 mg/kg (skin-on fillet sample). This suggests that the contribution of PCB uptake

during the smolt stream residence stage was 1.1% of the total PCBs body burden in the

adult skin-on fillet, while the remainder of the PCB burden was accumulated in Lake

Michigan. Analysis of coho salmon data yields similar results. The concentration of

PCBs found in adult steelhead trout and salmon in the Sheboygan River are consistent

with the tissue burdens reported in studies by DeVault et al. (1996) and Stow et al.

(1994), which evaluated PCB concentrations in coho and chinook salmon in Lake

Michigan.

POTENTIAL FOR PCBS FROM THE SHEBOYGAN RIVER TO IMPACT LAKE

MICHIGAN SALMONIDS

The results of studies that have evaluated PCB loading, tissue burdens, and biological

effects in salmonids of Lake Michigan indicate the following conclusions can be drawn:

• The Sheboygan River contributes approximately 2 to 4% of the load of PCBs

to Lake Michigan, or 21 to 24 kg/year (Marti and Armstrong 1990; Robertson

1996; Hall pers. comm. 1998).

• PCBs accumulate in the tissues of adult salmonids residing in Lake Michigan.

Concentrations have steadily declined from a peak in the 1970s to a leveling

off in the 1980s. The leveling off may be a new equilibrium; similar trends

have also been observed in forage species that are salmonid prey.

• PCBs bioaccumulate to the highest concentrations in lake trout, followed by

chinook salmon and coho salmon. The concentration of PCBs in steelhead

trout appears to be similar to coho salmon.

• A strong correlation has been found between PCB concentrations in eggs of

lake trout and chinook salmon and egg hatchability.

• No correlation was found between PCB concentrations and fry mortality.

• Lake trout appear to be more sensitive than chinook salmon.
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November 1998 7

• Coho salmon and steelhead trout smolts planted in the Sheboygan River could

accumulate substantial PCB concentrations before outmigration. The resultant

high PCB body burden could pose a significant risk to piscivores. Between

96 and 99% of coho salmon and steelhead trout adult body burden is

accumulated during their residence in Lake Michigan, although Lake Michigan

fish residing in the vicinity of the river mouth may accumulate higher body

burdens of PCBs.

Eggold et al. (1996) found that because of the short residence time that smolts spend in

the river compared with Lake Michigan, and the growth dilution that occurs in the lake as

the fish grow to adults, the tissue burdens accumulated in the river made up only 1 to 4%

of the total PCB burden in adult steelhead trout and coho salmon. Further, adult coho

salmon and steelhead trout that were originally planted in the Sheboygan River had no

higher tissue burdens as adults than in Lake Michigan populations as a whole (Eggold et

al. 1996).

In summary, the purpose of this appendix discussion is to evaluate whether salmonids

within Lake Michigan are at risk from PCBs originating from the Sheboygan River,

whether the PCBs are accumulated in the river prior to outmigration or in the lake as a

result of loading from the river. Three issues are of concern:

1) Are these body burdens sufficient to cause effects in the outmigrating smolts?

2) Are smolts suffering reproductive impacts as adults?

3) Are these body burdens sufficient to cause impacts on piscivorous fish or

wildlife that may consume them?

Based on the studies summarized in Table 4-15 of the risk assessment, smolts are unlikely

to be impacted directly at these concentrations. Since these smolts or their parents were

not reared in the Sheboygan (they were reared in hatcheries), most of their exposure to

PCBs took place after the smolts were past their most sensitive life stage. Effects in

fingerlings are not observed until body burdens are much higher. For example, Mauck et

al. (1978) reported mortality in fry containing 125 mg/kg ww Aroclor 1254.

As discussed in the previous section, returning steelhead trout and coho salmon that have

resided two to three years in Lake Michigan have lower PCB body burdens (maximum of

0.73 mg/kg ww). These concentrations are greater than some of the lowest effects

concentrations (as low as 0.12 mg/kg ww in flounder ovaries; see Table 4-15 in the risk

assessment) so reproductive effects cannot be ruled out, although they are somewhat

unlikely (see Section 4.6). While PCBs from the Sheboygan River contribute
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November 1998 8

approximately 1 to 4% of the returning salmonid tissue burden, this does not present high

enough PCB concentrations to alone drive risk to Lake Michigan salmonids. Returning

adults are not likely to experience adverse reproductive effects solely as a result of

exposure to concentrations of PCBs originating from the Sheboygan River.

Piscivorous wildlife and fish may consume these smolts or other young fish, which may

contain relatively high concentrations of PCBs, in the Sheboygan River or soon after they

outmigrate to Lake Michigan. As described in Section 5.0 of the risk assessment,

evaluating risks to these species is dependent on many factors, including the overall

percentage of diet that is made up of smolts.

REFERENCES

Ankley, G.T., D.E Tillitt, J.P. Giesy, P.D. Jones, D.A. Berbrugge. 1991. Bioassay derived

2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents in PCB-containing extracts from

flesh and eggs of Lake Michigan chinook salmon (Oncorhynchus tshawytscha)

and possible implications for reproduction. Can. J. Fish. Aquat. Sci. 48:1685-

1690.

DeVault, D.S., R. Hesselberg, P.W. Rodgers, and T.J. Feist. 1996. Contaminant trends in

lake trout and walleye from the Laurentian Great Lakes. J. Great Lakes Res.

22(4):884-895.

Eggold, B.T., J.F. Amrhein, and M.A. Cosbun. 1996. PCB accumulation by salmonine

smolts and adults in Lake Michigan and its tributaries and its effect on stocking

policies. J. Great Lakes Res. 22(2):403-413.

Hall, D. Personal communication (telephone conversation with Todd Goeks, National

Oceanic and Atmospheric Administration, on November 10, 1998 regarding PCB

load from Sheboygan River to Lake Michigan). U.S. Geological Survey,

Middleton, WI.

Leatherland, J.F. 1993. Field observations on reproductive and developmental

dysfunction in introduced and native salmonids from the Great Lakes. J. Great

Lakes Res. 19(4):737-751.

Mac, M.J., T.R. Schwartz, C.C. Edsall, and A.M. Frank. 1993. Polychlorinated

biphenyls in Great Lakes trout and their eggs: Relations to survival and congener

composition, 1979-1988. J. Great Lakes Res. 19(4):752-765.

Marti, E.A. and E.E. Armstrong. 1990. Polychlorinated biphenyls in Lake Michigan

tributaries. J. Great Lakes Res. 16:396-405.
C:\SHEBOYGAN PDF\G-SALMON\APPEND-G.DOC

November 1998 9

Mauck, W.L., P.M. Mehrle, and R.L. Mayer. 1978. Effects of the polychlorinated

biphenyl Aroclor 1254 on growth, survival, and bone development in brook trout

(Salvelinus fontinalis). J. Fish. Res. Board Can. 35:1084-1088.

Newsted, J.L. 1991. Biochemical effects of planar halogenated hydrocarbons on rainbow

trout (Oncorhynchus mykiss). Ph.D. dissertation, Michigan State University, East

Lansing, Michigan.

Oliver, B.G. and A.J. Niimi. 1988. Trophodynamic analysis of polychlorinated biphenyl

congeners and other chlorinated hydrocarbons in the Lake Ontario ecosystem.

Environ. Sci. Technol. 22(4):388-397.

Rasmussen, J.B., D.J. Rowan, D.R.S. Lean, and J.H. Carey. 1990. Food chain structure

in Ontario lakes determines PCB levels in lake trout (Salvelinus namaycush) and

other pelagic fish. Can. J. Fish. Aquat. Sci. 47:2030-2038.

Robertson, D.M. 1996. Use of frequency-volume analyses to estimate regionalized

yields and loads of sediment, phosphorus, and polychlorinated biphenyls to Lakes

Michigan and Superior. U.S. Geological Survey, Water Resources Investigations

Report 96-4092. Madison, WI.

Safe, S. 1990. Polychlorinated biphenyls (PCBs), dibenzo-p-dioxins (PCDDs), and

dibenzofurans (PCDFs) and related compounds: Environmental and mechanistic

considerations which support the development of toxic equivalency factors

(TEFs). Crit. Rev. Toxicol. 21:51-88.

Stow, C.A., S.R. Carpenter, and J.F. Amrhein. 1994. PCB concentration trends in Lake

Michigan coho (Oncorhynchus kisutch) and chinook salmon (O. tshawytscha).

Can. J. Fish. Aquat. Sci. 51:1384-1390.

Stow, C.A., S.R. Carpenter, L.A. Eby, J.F. Amrhein, and R.J. Hesselberg. 1995.

Evidence that PCBs are approaching stable concentrations in Lake Michigan

fishes. Ecological Applications. 5(1):248-260.

Tillitt, D.E., J.P. Giesy, and G.T. Ankley. 1991. Characterization of the H4IIE rat

hepatoma cell bioassay as a tool for assessing toxic potency of planar halogenated

hydrocarbons in environmental samples. Environ. Sci. Technol. 25:87-92.

Williams, L.L., and J.P. Giesy. 1992. Relationships among concentrations of individual

polychlorinated biphenyl (PCB) congeners, 2,3,7,8-tetrachlorodibenzo-p-dioxin

equivalents (TCDD-EQ), and rearing mortality of chinook salmon (Oncorhynchus

tshawytscha) eggs from Lake Michigan. J. Great Lakes Res. 18:108-124.
APPENDIX H

Recommendations for Long-Term Monitoring
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November 1998 1

APPENDIX H

RECOMMENDATIONS FOR LONG-TERM MONITORING

This appendix provides an outline of recommendations for a long-term monitoring

program in the Sheboygan River and Harbor that can be used to evaluate the

effectiveness of the selected remedial alternative in reducing risk to fish and wildlife

from exposure to polychlorinated biphenyls (PCBs). This monitoring program should be

developed in coordination with the U.S. Environmental Protection Agency (USEPA), the

Wisconsin Department of Natural Resources (WDNR), and the natural resource trustee

agencies. Recommendations are made in this appendix to serve as a starting point for

developing the program. The proposed monitoring program focuses on PCB

concentrations in fish and sediment, because the greatest risks identified in the

Sheboygan River and Harbor Aquatic Ecological Risk Assessment (ERA) were associated

with PCB exposure to fish and wildlife. There are two basic objectives of the monitoring

program:

• Provide the basis for evaluating the effectiveness of remedial actions by

supplementing existing site data to establish a comprehensive pre-remediation

baseline

• Generate data for periodic re-evaluation of potential fish, piscivorous wildlife,

and human exposure to residual PCBs and associated risks

The primary recommendations for the monitoring program include three main

components:

1) Resident fish monitoring of adult and young-of-year or juvenile fish

2) Caged fish studies using fathead minnows; these studies would be used to

gather the data necessary to assess the contribution of PCBs in the water

column to the observed PCB body burdens in resident fish

3) Sediment sampling

These recommendations were based in part on the availability of existing data from the

Interim Monitoring Program (BBL 1996), the WDNR food chain study, and the ERA.

Biological monitoring of floodplain areas, based on recommendations of the forthcoming

floodplain ecological risk assessment, should also be incorporated in the monitoring

program. The plan for conducting the baseline monitoring event should be developed

and implemented, at a minimum, one year prior to remedial action, recognizing that

detailed monitoring programs for all components of the monitoring program may not be

developed prior to the completion of the remedial design. The preliminary recommended
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November 1998 2

approach described below is compared to past monitoring and study efforts in Table H-1.

Note that these details may change during discussions with other agencies.

Resident fish would be collected in late summer from the four river segments sampled in

the ERA and other sampling efforts, Segments 1, 2, 3, and 5. Adult (> 9 in.) fish would

be collected from each of the four sampling segments and analyzed for low resolution

PCB congeners, moisture content, and lipids. Some of the fish would also be analyzed

for high resolution PCB congeners. Adult fish would be collected twice during the

baseline sampling event to look for a seasonal influence. Composite samples of youngof-year or juvenile (2–6 in.) fish would also be collected in each of the four segments and

analyzed for low resolution PCB congeners. Some of the young-of-year or juvenile fish

composite samples would also be analyzed for high resolution PCB congeners. The

collection schedule would be determined in cooperation with other agencies and would

need to be consistent from year to year.

Caged fathead minnows (0.3–1 g each) would be deployed in the same period each year

(e.g., July or August) at each of the same 5 sites used in the Interim Monitoring Program

(BBL 1996), unless remedy selection and feasibility study results indicate other areas of

concern. Three vessels would be deployed at each site with 150 fish in each; only two

vessels would be sampled. The third vessel would be for contingencies. Three

composite samples would be collected from each of two vessels after three weeks of

exposure and analyzed for low resolution PCB congeners, moisture content, and lipids.

A three week exposure period appears to be sufficient based on the results of Rice and

White (1987) and Jones and Sloan (1989) and is less likely to stress the fish.

For the sediment component, baseline sediment sampling would be accomplished during

the predesign sampling event. The sampling and analysis plan for this event would be

reviewed to ensure method compatibility. Long-term monitoring would begin with the

verification sampling event following remedy implementation. A plan for continued

sediment monitoring would be completed at that time.

Comments were received from other agencies during their review of this document in

draft format. Suggested monitoring program elements included water column

monitoring, snapping turtle monitoring, sediment traps, and analysis of fish eggs. The

final program should be developed in consultation with all involved agencies, and a

baseline sampling event should be conducted before any response actions are taken at the

site.
Table H-1. Recommended monitoring approach compared to the ERA,

the WDNR food chain study, and the Interim Monitoring Program

Adult Fish

(e.g., smallmouth bass)

Young-of-year/Juvenile Fish

(e.g., smallmouth bass or white 

sucker)

Young-of-year/Juvenile Fish

(e.g., white sucker) Caged Fish

Sampling Location 

Recommendation

Segments 1, 2, 3, 5 (only 

Segments 2, 3, and 5 were 

sampled in IMP 1994, 

1995)

Segments 1, 2, 3, 5 Segments 1, 2, 3, 5 5 stations 

(see Figure H-1)

Size Range

ERA na 5-8 inches na na

WDNR food chain study 8-12 inches 2-4 inches 2-5 inches na

IMP Work Plan > 9 inches na 3-8 inches Not specified

IMP 1994, 1995 9-15 inches na 3-6 inches 0.3-1.0 g, average weight 

per fish

Recommendation >9 inches (WDNR 

suggestion; average weight 

250 g)

2-6 inches (target 2-4 inches) 2-6 inches (target 2-4 inches) 0.3-1 g to be consistent 

with IMP 1994, 1995

Sampling Schedule

ERA na Mid August na na

WDNR food chain study October 13-24, 1994 October 13-24, 1994 October 13-24, 1994 na

IMP Work Plan Not specified na Not specified Not specified

IMP 1994, 1995 Late September na Late September Deployed mid to late 

September

Recommendation To be determined in 

consultation with agencies 

involved

To be determined in 

consultation with agencies 

involved

To be determined in 

consultation with agencies 

involved

Deploy in July or August 

(check for seasonal 

influence in baseline)
Table H-1. Recommended monitoring approach compared to the ERA,

the WDNR food chain study, and the Interim Monitoring Program

Adult Fish

(e.g., smallmouth bass)

Young-of-year/Juvenile Fish

(e.g., smallmouth bass or white 

sucker)

Young-of-year/Juvenile Fish

(e.g., white sucker) Caged Fish

Number of Fish and Composites per Location

ERA na 11-14 fish total; 3-5 fish per 

composite; 3 composites

na

WDNR food chain study 9-12 fish total; 3-4 fish per 

composite; 3 composites

18-24 fish total; 6-8 fish per 

composite; 3 composites

18-27 fish total; 6-9 fish per 

composite; 3 composites

IMP Work Plan 12 fish analyzed 

individually (per 

compromise with WDNR)

na Unclear if 25 individual fish or 

composites

2 vessels with 250 fish in 

each; duplicate 10-g 

composites of 20-25 fish 

collected from each vessel 

on 21st and 42nd days

IMP 1994, 1995 12 fish analyzed 

individually

na 50 fish total; 2 fish per 

composite; 25 composites (not 

enough fish collected for 

25 composites in 1995 at 

2 stations); not analyzed to date

200 fish in each of 

2 vessels; duplicate 

composite samples 

collected from each vessel 

at 3 and 6 wks (insufficient 

sample volume in 1995)

Recommendation To be determined in 

consultation with agencies 

involved

To be determined in 

consultation with agencies 

involved

To be determined in 

consultation with agencies 

involved

150 fish in each of 

2 vessels (deploy an 

additional vessel with 

150 fish for backup); 

3 composites of 20-25 fish 

collected from each vessel 

at 3 wks (may need more 

fish to obtain sufficient 

sample for analysis)
Table H-1. Recommended monitoring approach compared to the ERA,

the WDNR food chain study, and the Interim Monitoring Program

Adult Fish

(e.g., smallmouth bass)

Young-of-year/Juvenile Fish

(e.g., smallmouth bass or white 

sucker)

Young-of-year/Juvenile Fish

(e.g., white sucker) Caged Fish

PCB Analysis

ERA (congeners) na Axys (1997) method/USEPA 

method 1668

na na

WDNR food chain study 

(congeners)

Wisconsin State Laboratory 

of Hygiene (SLOH) Method 

1410

SLOH Method 1410 SLOH Method 1410 na

IMP Work Plan (Aroclors) WDNR procedure 1400 

(Ribick et al. 1982)

na WDNR procedure 1400 (Ribick 

et al. 1982)

WDNR procedure 1400 

(modified extraction [HES 

1990])

IMP 1994, 1995 (Aroclors) Information not available 

(method should be 

presented in BBL 1996a)

na Information not available 

(method should be presented in 

BBL 1996a)

Information not available 

(method should be 

presented in BBL 1996a)

Recommendation Axys (1997) 

method/USEPA method 

1668 (low resolution 

congener analysis of all 

samples; high resolution 

analysis on 3 samples)

Axys (1997) method/USEPA 

method 1668 (low resolution 

congener analysis of all 

samples; high resolution 

analysis on 3 samples)

Axys (1997) methods (low 

resolution congener analysis on 

all samples)

Axys (1997) methods (low 

resolution congener 

analysis on all samples)

Need to also analyze a standard reference material and perform a cross check between different laboratories and methods. 
Table H-1. Recommended monitoring approach compared to the ERA,

the WDNR food chain study, and the Interim Monitoring Program

Adult Fish

(e.g., smallmouth bass)

Young-of-year/Juvenile Fish

(e.g., smallmouth bass or white 

sucker)

Young-of-year/Juvenile Fish

(e.g., white sucker) Caged Fish

Lipid Analysis

ERA na Modified Bligh and Dyer 1959 

(methylene chloride)

na na

WDNR food chain study Not presented in QAPP 

(WDNR 1995)

Not presented in QAPP (WDNR 

1995)

Not presented in QAPP (WDNR 

1995)

na

IMP Work Plan WDNR procedure 1400 

(Ribick et al. 1982)

na WDNR procedure 1400 (Ribick 

et al. 1982)

WDNR procedure 1400 

(Ribick et al. 1982)

IMP 1994, 1995 Information not available 

(method should be 

presented in BBL 1996a)

na Information not available 

(method should be presented in 

BBL 1996a)

Information not available 

(method should be 

presented in BBL 1996a)

Recommendation Modified Bligh and Dyer 

1959

Modified Bligh and Dyer 1959 Modified Bligh and Dyer 1959 Modified Bligh and Dyer 

1959

Grams of tissue required for 

recommendation

aliquot from PCB extract aliquot from PCB extract aliquot from PCB extract aliquot from PCB extract

Exposure Period

IMP Work Plan na na na 3 and 6 week collection 

(analyze at 6 weeks)

IMP 1994, 1995 na na na 3 and 6 week collections 

and analysis; 3 vs 6 week 

concentrations varied 

(equal in 1994 and 2x in 

1995)

Recommendation na na na 3 week collection and 

analysis

SOURCE: NOTE:

ERA - Sheboygan River and Harbor Aquatic Ecological Risk Assessment IMP - Interim Monitoring Program

WDNR food chain study - WDNR (1996) na - not applicable

IMP Work Plan - BBL (1996a) QAPP - quality assurance project plan

IMP 1994 - BBL (1995) WDNR - Wisconsin Department of Natural Resources

IMP 1995 - BBL (1996b)
Sheboygan

Falls Dam

A

B

C

River

Bend Dam

Rochester

Park

USGS

Gaging

Station

0 0.5 1 kilometer0 0.5 1 mile

N

Kohler

Esslinge

Park

Sh eboygan Rive

r

Chicago a

RM12 RM11

RM9

RM8

RM7

RM6

RM5

RM4

Tecumseh

Products

Company

Kohler

Landfill

Mullet Ri ver

PP

PP

23

23

43

Ontario

IN

IA

IL

MI

MI

WI

MN

L

a

k

e

M

i

c

h

i g

a

n

L a k e S u p er i o r

Sheboygan

Figure H-1. Location of previous caged fish samples for the Interim Monitoring Program

2/575-37\DELIVER/ERA/GRAPHICS/FINAL/FIG.H-1November 1998

Segment 2

Segment 4

Segment 3

Seg

Segment 1

RM10

Waelderhaus

Dam

On

i

on River

Sheboygan

Falls

RM13

D

E

7
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November 1998 8

REFERENCES

Axys. 1997. Analytical methods manual, current routine methods: Aroclors, total PCBs,

chlorinated pesticides, PCB congeners, coplanar PCBs (non-ortho-substituted),

toxaphene, chlorobenzenes. Axys Analytical Services Ltd. Sidney, BC, Canada.

BBL. 1995. 1994 annual interim monitoring report. Blasland, Bouck & Lee, Inc.,

Syracuse NY.

BBL. 1996a. Work plan/QAPP, interim monitoring program, Sheboygan River and

Harbor. Blasland, Bouck & Lee, Inc., Syracuse NY.

BBL. 1996b. 1995 annual interim monitoring report. Blasland, Bouck & Lee, Inc.,

Syracuse NY.

Bligh, E.G. and W.J. Dyer. 1959. A rapid method of total lipid extraction and

purification. Can. J. Biochem. Physiol. 37:911-917.

HES. 1990. Extraction method MP-BPCB-MA. Hazleton Environmental Services, Inc.,

Madison, WI.

Jones, P.A. and R.J. Sloan. 1989. An in situ river exposure vessel for bioaccumulation

studies with juvenile fish. Environ. Toxicol. Chem. 8:151-155.

Ribick, M., Petty, and Stalling. 1982. Toxaphene residues in fish: Identification,

quantification and confirmation at part per billion levels. Environ. Sci. Technol.

16:310-318.

Rice, C.P. and D.S. White. 1987. PCB availability assessment of river dredging using

caged clams and fish. Environ. Toxicol. Chem. 6:259-274.

WDNR. 1995. Sheboygan River food chain and sediment contaminant assessment

quality assurance project plan. Wisconsin Department of Natural Resources,

Madison, WI.

WDNR. 1996. Data collected for the Sheboygan River food chain and contaminant

assessment. Wisconsin Department of Natural Resources, Southeast District

Water Resources Management Section.
APPENDIX I

Analytical Methods
C:\SHEBOYGAN PDF\APPEND-I.DOC

November 1998 1

APPENDIX I

ANALYTICAL METHODS

Methods used for the analysis of sediment and tissue collected in August 1997 as part of

the Sheboygan River and Harbor Aquatic Ecological Risk Assessment are presented in

this Appendix. A summary of the data quality objectives, including the specific analytical

methods used, is presented in Table I-1 and the targeted detection limits are presented in

Table I-2.

All methods are readily accessible in common procedural literature (e.g.,

U.S. Environmental Protection Agency methods) except the method used by Axys

Analytical Services Ltd. for the low resolution analysis of PCB congeners. This method

is provided in Attachment I-1.
Table I-1. Summary of data quality objectives

PARAMETER UNITS METHOD DETECTION LIMIT SAMPLE SIZE PRECISION ACCURACY COMPLETENESS METHOD REFERENCE LAB

Sediment

Total Metals mg/kg

(dry weight)

0.15–95 50 g ±35% ±35% 95% GFAA/ ICP USEPA

6010/700

ARI

Mercury mg/kg

(dry weight)

0.05 Aliquot from

total metals

±35% ±35% 95% CVAA USEPA Method 7471 ARI

Pesticides/

PCBs

μg/kg

(dry weight)

0.6–7 150 g ±50% ±50% 95% GC/ECD USEPA

8081

ARI

PCB Congeners pg/g

(dry weight)

0.1 30 g ±50% 70-120% 95% GC/MS Axys

CL-S-01 /Ver.2

Axys

Toxic PCB

Congeners

pg/g

(dry weight)

0.2–1.5 30 g ±50% 70-120% 95 % HRGC/HRMS USEPA Method 1668 Axys

Dioxins and

Furans

pg/g

(dry weight)

0.05–0.8 30 g ±50% 70-120% 95% HRGC/HRMS USEPA Method

1613B

Axys

PAHs μg/kg

(dry weight)

10 150 g ±50% ±50% 95% GC/MS-SIM Modified USEPA

8270

ARI

Total organic

carbon

%

(dry weight)

0.1 25 g ±20% ±20% 95% Combustion PSEP 1986;

Standard Method

5310B

ARI

Acid-volatile

sulfides

μmole/g 0.9 10 g ±30% ±25% 95% Distillation,

titration

Allen et al. 1991 ARI

Simultaneously

extracted metals

μmole/g 0.02–0.5 From AVS

extract

±35% ±35% 95% ICP/GFAA Modified CLP after

extraction

ARI

Moisture

Content

% 0.1 50 g ±10% ±20% 95% Combustion USEPA Method

160.3

ARI

Grain size 0.0001

(dry weight)

na 500 g ±30% na 95% Sieve ASTM D422 Soil Tech.
Table I-1, continued

PARAMETER UNITS METHOD DETECTION LIMIT SAMPLE SIZE PRECISION ACCURACY COMPLETENESS METHOD REFERENCE LAB

Tissue

Total Metals mg/kg

(as received)

0.025–20 10 g ±35% ±35% 95% GFAA/ ICP USEPA

6010/700

ARI

Mercury mg/kg

(as received)

0.005 Aliquot from

total metals

±35% ±35% 95% CVAA USEPA Method 7471 ARI

Pesticides/

PCBs

μg/kg

(as received)

17–100 30 g ±50% ±50% 95% GC/ECD Modified USEPA

8081

ARI

PCB

Congeners

pg/g

(as received)

0.1 30 g ±50% 70-120% 95% GC/MS Axys

CL-T-02 /Vers. 2

Axys

Toxic PCB

Congeners

ng/g

(as received)

0.1 30 g ±50% 70-120% 95% HRGC/HRMS USEPA Method 1668 Axys

PAHs μg/kg

(as received)

7 20 g ±50% ±50% 95% GC/MS-SIM PSEP 1997 ARI

Dioxins and

Furans

pg/g

(as received)

0.05–0.8 30 g ±50% 70-120% 95% HRGC/HRMS USEPA Method

1613B

Axys

Moisture

Content

% 0.1 20 g ±10% ±20% 95% Combustion USEPA Method

160.3

ARI

Percent lipids % na Aliquot from

PAH

±30% na 95% Gravimetric Bligh and Dyer 1959 ARI

NOTE: ARI - Analytical Resources, Inc.

Axys - Axys Analytical Services, Ltd.

CVAA - cold vapor atomic absorption

GC/ECD - gas chromatography/electron captured detector

GC/MS - gas chromatography/mass spectrometry

GFAA - graphite furnace atomic absorption

HRGC/HRMS - High resolution gas chromatography/high resolution mass spectrometry

ICP - inductively coupled plasma atomic emission spectrometry

na - not applicable

PAH - polycyclic aromatic hydrocarbon

PCB - polychlorinated biphenyl

PSEP - Puget Sound Estuary Program

SIM - selected ion monitoring

Soil Tech. - Soil Technology, Inc.

USEPA - U.S. Environmental Protection Agency
Table I-2. Targeted detection limits

C:\SHEBOYGAN PDF\APPEND-I.DOC

November 1998 4

ANALYTE SEDIMENT FISH TISSUE

PAHsa (μg/kg)

Acenaphthylene 7 na

Acenaphthene 7 na

Fluorene 7 na

Phenanthrene 7 na

Anthracene 7 na

Fluoranthene 7 na

Pyrene 7 na

Benz(a)anthracene 7 na

Chrysene 7 na

Benzo(b)fluoranthene 7 na

Benzo(k)fluoranthene 7 na

Benzo(a)pyrene 7 na

Indeno(1,2,3-cd,)pyrene 7 na

Naphthalene 7 na

Pesticides/PCBsb (μg/kg)

Gamma-BHC Lindane 1.0 na

Heptachlor epoxide 1.0 na

Dieldrin 2.0 na

P,p'-DDE 2.0 na

Endrin 2.0 na

P,p'-DDD 2.0 na

P,p' -DDT 2.0 na

Chlordane 1.0 na

Total DDT 1.0 na

PCBs (each Aroclor) 20 na

Aroclor 1221 40 na

Metalsc (mg/kg)

Arsenic 5 na

Cadmium 0.25 na

Chromium 17 na

Copper 12 na

Lead 17 na

Mercury 0.075 na

Nickel 7 na

Silver 0.5 na

Zinc 47 na

PCB Congeners (BZ no.)d (ng/g wet weight)

2,3/2,4′ (5/8) 0.1 0.1

4,4′ (15) 0.1 0.1

2,2′,3/2,4′,6 (16/32) 0.1 0.1

2,2′,4 (17) 0.1 0.1

2,2′,5 (18) 0.1 0.1

2,2′,6 (19) 0.1 0.1

2,3,4′ (22) 0.1 0.1

2,3,6/2,3′,6 (24/27) 0.1 0.1
Table I-2. continued

C:\SHEBOYGAN PDF\APPEND-I.DOC

November 1998 5

ANALYTE SEDIMENT FISH TISSUE

2,3′,4 (25) 0.1 0.1

2,3′,5 (26) 0.1 0.1

2,4,4′/2,4′,5 (28/31) 0.1 0.1

2′,3,4 (33) 0.1 0.1

2,2′,3,3′ (40) 0.1 0.1

2,2′,3,4/2,3,4′,6/2,3′,4′,6 (41/64/71) 0.1 0.1

2,2′,3,4′ (42) 0.1 0.1

2,2′,3,5′ (44) 0.1 0.1

2,2′,3,6 (45) 0.1 0.1

2,2′,3,6′ (46) 0.1 0.1

2,2′,4,4′/2,2′4,5 (47/48) 0.1 0.1

2,2′,4,5′ (49) 0.1 0.1

2,2′,5,5′ (52) 0.1 0.1

2,3,3′,4′/2,3,4,4′ (56/60) 0.1 0.1

2,3′,4,5′ (68) 0.1 0.1

2,3′,4′,5/2′,3,4,5 (70/76) 0.1 0.1

2,4,4′,5 (74) 0.1 0.1

2,2′,3,3′,5 (83) 0.1 0.1

2,2′,3,3′,6/2,2′,3,4,6′ (84/89) 0.1 0.1

2,2′,3,4,4′ (85) 0.1 0.1

2,2′,3,4,5′ (87) 0.1 0.1

2,2′,3,4′,5/2,2′,4,5,5′ (90/101) 0.1 0.1

2,2′,3,4′,6 (91) 0.1 0.1

2,2′,3,5′,6 (95) 0.1 0.1

2,2′,3′,4,5 (97) 0.1 0.1

2,2′,4,4′,5 (99) 0.1 0.1

2,3,3′,4′,5 (107) 0.1 0.1

2,3,3′,4′,6 (110) 0.1 0.1

2,2′,3,3′,4,4′ (128) 0.1 0.1

2,2′,3,3′,4,5 (129) 0.1 0.1

2,2′,3,3′,4,5′ (130) 0.1 0.1

2,2′,3,3′,4,6 (131) 0.1 0.1

2,2′,3,3′,5,6 (134) 0.1 0.1

2,2′,3,3′,5,6′/2,2′,3,4,5′,6 (135/144) 0.1 0.1

2,2′,3,3′,6,6′ (136) 0.1 0.1

2,2′,3,4,4′,5 (137) 0.1 0.1

2,2′,3,4,4′,5′/2,3,3′,4′,5,6/2,3,3′,4′,5′,6 (138/163/164) 0.1 0.1

2,2′,3,4,5,5′ (141) 0.1 0.1

2,2′,3,4,5′,6 (144) 0.1 0.1

2,2′,3,4′,5,5′ (146) 0.1 0.1

2,2′,3,4′,5′,6 (149) 0.1 0.1

2,2′,3,5,5′,6 (151) 0.1 0.1

2,2′,4,4′,5,5′ (153) 0.1 0.1

2,3,3′,4,4′,5′ (157) 0.1 0.1

2,3,3′,4,4′,6 (158) 0.1 0.1

2,2′,3,3′,4,4′,5/2,3,3′,4,4′,5,6 (170/190) 0.1 0.1

2,2′,3,3′,4,4′,6 (171) 0.1 0.1
Table I-2. continued

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November 1998 6

ANALYTE SEDIMENT FISH TISSUE

2,2′,3,3′,4,5,5′ (172) 0.1 0.1

2,2′,3,3′,4,5,6′ (174) 0.1 0.1

2,2′,3,3′,4,5′,6 (175) 0.1 0.1

2,2′,3,3′,4,6,6′ (176) 0.1 0.1

2,2′,3,3′,4′,5,6 (177) 0.1 0.1

2,2′,3,3′,5,5′,6 (178) 0.1 0.1

2,2′,3,3′,5,6,6′ (179) 0.1 0.1

2,2′,3,4,4′,5,6′/2,2′,3,4′,5,5′,6 (182/187) 0.1 0.1

2,2′,3,4,4′,5′,6 (183) 0.1 0.1

2,2′,3,4,5,5′,6 (185) 0.1 0.1

2,3,3′,4,4′,5′,6 (191) 0.1 0.1

2,3,3′,4′,5,5′,6 (193) 0.1 0.1

2,2′,3,3′,4,4′,5,5′ (194) 0.1 0.1

2,2′,3,3′,4,4′,5,6 (195) 0.1 0.1

2,2′,3,3′,4,4′,5,6′ (196) 0.1 0.1

2,2′,3,3′,4,4′,6,6′ (197) 0.1 0.1

2,2′,3,3′,4,5,5′,6 (198) 0.1 0.1

2,2′,3,3′,4,5,6,6′ (199) 0.1 0.1

2,2′,3,3′,4,5,5′,6′ (201) 0.1 0.1

2,2′,3,4,4′,5,5′,6 (203) 0.1 0.1

2,3,3′,4,4′,5,5′,6 (205) 0.1 0.1

2,2′,3,3′,4,4′,5,5′,6 (206) 0.1 0.1

2,2′,3,3′,4,4′,5,6,6′ (207) 0.1 0.1

2,2′,3,3′,4,5,5′,6,6′ (208) 0.1 0.1

2,2′,3,3′,4,4′,5,5′,6,6′ (209) 0.1 0.1

Toxic PCB Congeners (BZ no.)d,e (pg/g wet

weight)

3,3′,4,4′ (77) 0.23 0.23

2′,3,4,4′,5 (123) 0.50 0.50

2,3′,4,4′,5 (118) 0.50 0.50

2,3,4,4′,5 (114) 0.50 0.50

2,3,3′,4,4′ (105) 0.50 0.50

3,3′,4,4′,5 (126) 0.50 0.50

2,3′,4,4′,5,5′ (169) 1.00 1.00

2,3,3′,4,4′,5 (156) 1.00 1.00

2,3′,4,4′,5,5′ (167) 1.00 1.00

2,2′,3,4,4′,5,5′ (180) 1.00 1.00

2,2′,3,3′,4,4′,5 (170) 1.00 1.00

2,3,3′,4,4′,5,5′ (189) 1.00 1.00

Dioxins/furanse

 (pg/g wet weight) 0.2–1.5 0.2–1.5

2,3,7,8-TCDD 0.02 0.02

1,2,3,7,8-PeCDD 0.04 0.04

1,2,3,4,7,8-HxCDD 0.06 0.06

1,2,3,6,7,8-HxCDD 0.06 0.06

1,2,3,7,8,9-HxCDD 0.06 0.06

1,2,3,4,6,7,8-HpCDD 0.15 0.15

OCDD 0.25 0.25
Table I-2. continued

C:\SHEBOYGAN PDF\APPEND-I.DOC

November 1998 7

ANALYTE SEDIMENT FISH TISSUE

2,3,7,8-TCDF 0.02 0.02

1,2,3,7,8-PeCDF 0.04 0.04

2,3,4,7,8-PeCDF 0.06 0.06

1,2,3,4,7,8-HxCDF 0.06 0.06

1,2,3,6,7,8-HxCDF 0.06 0.06

1,2,3,7,8,9-HxCDF 0.06 0.06

2,3,4,6,7,8-HxCDF 0.06 0.06

1,2,3,4,6,7,8-HpCDF 0.15 0.15

1,2,3,4,7,8,9-HpCDF 0.15 0.15

OCDF 0.25 0.25

Total Tetra-dioxins 0.02 0.02

Total Penta-dioxins 0.04 0.04

Total Hexa-dioxins 0.06 0.06

Total Hepta-dioxins 0.15 0.15

Total Tetra-furans 0.02 0.02

Total Penta-furans 0.04 0.04

Total Hexa-furans 0.06 0.06

Total Hepta-furans 0.15 0.15

Acid Volatile Sulfides (μmole/g) 0.9 na

Simultaneously Extracted Metals (μmole/g) 0.02–0.5 na

Total Organic Carbon (%) 0.1

Moisture Content (%) 0.1 na

Percent Lipids na na

Grain Size 0.0001 na

NOTE: na - not applicable

PAH - polycyclic aromatic hydrocarbon

PCB - polychlorinated biphenyl

a PAHs in sediment and tissue are based on detection limits in the WDNR food chain study. This

detection limit for sediment is lower than threshold effects levels (TELs) for individual PAHs. The

detection limit for tissues is slightly higher than in the food chain study (7 :g/kg). The laboratory

(Analytical Resources, Inc. [ARI]) may be able to obtain a detection limit of 5 :g/kg barring any

matrix interferences.

b Pesticides/PCBs target detection limits in sediment are equal to the TEL (Smith et al. 1996; USEPA

1996). Pesticides/PCBs in mussel tissue are equal to the laboratory (ARI) detection limits.

c Metals in sediment are equal to the TEL, except for silver which is equal to the effects range low

(ERL). Metals in mussels are the lowest concentrations that were detected in crayfish or insects

during the WDNR food chain study for arsenic and copper. For other metals, the target detection

limits are equal to the laboratory (ARI) detection limit, because the lowest concentrations in the food

chain study could not be obtained (cadmium, chromium, lead, mercury, and silver), or previous data

were not available (nickel, zinc).

d Ballschmiter-Zell (BZ) number is in parentheses.

e

Laboratory (Axys Analytical Services, Inc.) detection limits. These are lower than the concentrations

associated with TCDD risk to fish in tissue (50 pg/g) and sediment (60 pg/g) (USEPA 1993).
C:\SHEBOYGAN PDF\APPEND-I.DOC

November 1998 8

REFERENCES

Allen, H.E., F. Gongmin, W. Boothman, D. Ditoro, and J.D. Mahony. 1991.

Determination of acid volatile sulfides and simultaneously extracted metals in

sediments. U.S. Environmental Protection Agency, Office of Science and

Technology, Washington, DC. 17 pp.

Bligh, E.G., and W.J. Dyer. 1959. A rapid method of total lipid extraction and

purification. Can. J. Biochem. Physiol. 37(8):911-917.

PSEP. 1986. Recommended protocols for measuring conventional sediment variables in

Puget Sound. Prepared for the U.S. Environmental Protection Agency, Region 10,

Seattle, WA. Puget Sound Water Quality Authority, Olympia, WA.

PSEP. 1997. Recommended guidelines for measuring organic compounds in Puget

Sound water, sediment and tissue samples. Prepared for the U.S. Environmental

Protection Agency, Region 10, Seattle, WA. Puget Sound Water Quality Action

Team, Olympia, WA.

Smith, S.L., D.D. MacDonald, K.A. Keenleyside, C.G. Ingersoll, and L.J. Field. 1996.

A preliminary evaluation of sediment quality assessment values for freshwater

ecosystems. J. Great Lakes Res. 22(3):624-638.

USEPA. 1996. Assessment and Remediation of Contaminated Sediments (ARCS)

Program: calculation and evaluation of sediment effect concentrations for the

amphipod Hyalella azteca and the midge Chironomus riparius. EPA 905-R96-

008. U.S. Environmental Protection Agency, Great Lakes National Program

Office, Chicago, IL.

USEPA. 1993. Interim report on data and methods for assessment of 2,3,7,8-

tetrachlorodibenzo-p-dioxin risks to aquatic life and associated wildlife.

EPA/600/R-93/055. U.S. Environmental Protection Agency, Office of Research

and Development, Washington, DC.
ATTACHMENT I-1

Axys Analytical Services Ltd.

Analysis of PCB congeners

in tissue and sediment/soil samples
AXYS ANALYTICAL SERVICES LTD.

ANALYSIS OF PCB CONGENERS IN TISSUE AND SEDIMENT/SOIL SAMPLES

All samples were spiked with an aliquot of surrogate standard solution containing

13C-labelled surrogates (see Table 1). Tissue samples were extracted by column elution and

sediment/soil samples were extracted by shaking with solvent. The raw extract was

fractionated and cleaned up on a Florisil column. The first fraction (F1) was analyzed by high

resolution gas chromatography with low resolution (quadrupole) mass spectrometric detection

(HRGC/LRMS) for PCBs as individual congeners.

1. Extraction

Tissue: A subsample of homogenized wet tissue (approximately 10 g wet for initial

analyses and 1 g wet for repeat analyses) was mixed with powdered anhydrous sodium

sulphate, spiked with an aliquot of the surrogate standard solution and loaded into a

chromatographic column. The sample was extracted by eluting the column with 100 mL

dichloromethane (DCM) at a rate of 3-5 mL per minute. One mL hexane was added to the

DCM and the extract was concentrated to about 5 mL. At this point the raw extract was

subsampled for gravimetric lipid determination.

The extract was loaded onto a calibrated Biobeads SX-3 column and eluted with 1:1

dichloromethane:hexane. The Biobeads column is calibrated for the separation of the

organochlorines of interest from lipids and other biogenic compounds. The extract was

concentrated prior to column cleanup and fractionation.

A separate subsample of tissue was used for gravimetric moisture determination.

Sediment/Soil: A subsample of wet sediment (approximately 10 to 15 g) was weighed

into a round bottom flask, spiked with an aliquot of the surrogate standard solution and

extracted by shaking with 1:1 methanol:dichloromethane (20 min., decanted) and

dichloromethane (20 min., decanted). The extracts were combined and the solvent backextracted with extracted, distilled water (twice). The extract was dried over anhydrous sodium

sulphate, reduced in volume and allowed to react with activated copper to remove sulphur.

A separate subsample of sediment was used for gravimetric moisture determination.

2. Column Chromatography For PCB Congeners

The extract was applied to a Florisil column for which cutpoints had been previously

determined. The column was eluted with hexane. The F1 fraction contained the majority of the

PCB congeners, (only a few coplanar PCBs not measured by this method elute into F2). F1

was concentrated to a small volume and sediment/soil extracts were again treated with

activated copper. All extracts were spiked with an aliquot of recovery standard solution

(

13C-labelled PCB 153) prior to instrumental analysis.
3. HRGC/LRMS Analysis Of PCB Congeners

The F1+F2 fraction was analyzed for PCBs as individual PCB congeners using a

Finnigan INCOS 50 mass spectrometer equipped with a Varian 3400 GC, a CTC autosampler

and a Prolab Envirolink system for MS control and data acquisition. Chromatographic

separation was achieved using a J&W DB-5 column (60 m x 0.25 mm i.d., o.25 μm film

thickness). The MS was operated at unit mass resolution in the EI mode using multiple ion

detection (MID) to enhanace sensitivity, acquiring at least two characteristic ions for each

target analyte and surrogate standard. The ions monitored were as described in Table 2. A

split/splitless injection sequence was used. Reported concentrations were corrected for the

recovery of the surrogate standards added prior to workup.

4. Quantification

Compounds were identified if the GC/MS data satisfied the following criteria:

i) The retention time of the peak was within three seconds of the predicted time from

that authentic compound in the calibration standard.

ii) Peak response of both ions was at least three times the background noise level.

iii) Peak maxima for both characteristic ions coincided within two seconds.

iv) The ratio of characteristic ion peak areas must have been within +/-20% of the value

found in the calibration standard.

Quantification was conducted using HP EnviroQuant and Prolab-MS Extend software

linked to Excel spreadsheets. The chromatograms supplied and the ProLab quantitation

summary sheets are provided to give areas and retention times only; the data values shown

are raw (absolute) numbers and final concentrations are to be taken from final data reports

only.

A five point calibration curve was used to determine instrument linearity prior to the

analysis of samples. The concentration of the identified compounds were calculated against

the surrogate standards thereby automatically correcting the data for the recovery of the

surrogates. Mean relative response factors, determined from the calibration standard runs

made before and after each batch of samples run were used for quantification. 

5. Calculations

Concentrations: The internal standard method was used to quantify all target analytes

in the samples. All data reported are corrected for the recovery of the surrogate. Conc i, the

concentration of a target analyte was calculated using the following equations:

i

i

si

si

i,si x

Conc = 

A

A

 x 

W

RRF

 x 

1

W
where Ai = area of the analyte peak of interest (compound i)

Asi = area of labelled surrogate used to quantify i

Wx = weight of sample taken for analysis

Wsi = weight of labelled surrogate (compound si) added to sample

RRFi,si = mean relative response factor of i to si from the bracketing

calibration runs and defined as

Detection Limits: Detection limits (DLi) were also calculated for each analyte using the

concentration equations given above, with the minimum detectable peak area used for Ai as

follows:

where AMINi = minimum detectable peak area in the channel of interest

and Asi, Wx, Wsi and RRFi,si as defined above.

AMINi is calculated from N, the maximum noise in the predetermined "window" on the

mass chromatogram channel of interest as follows:

where N was determined by the EnviroQuant/Prolab software; and As and Hs are the

area and the height of the peak for 13C-PCB 101.

The noise for each target analyte was determined from the actual chromatogram of the

sample of interest.

Surrogate Standard Recoveries: Recoveries of surrogate standards were calculated

using the following equations:

i

si

si

i

A

A

 x 

W

W

i

I

si

si

i,si x

DL = 

AMIN

A

 x 

W

RRF

 x 

1

W

i

s

s

AMIN = N x A

H

 x 3

%Recovery = 

A

A

 x 

W

RRF

 x 

1

W

 x 100 si

rs

rs

si,rs si
where Ars and Asi were the peak areas of recovery (internal) standard and labelled

surrogate added to the sample, Wrs and Wsi were the weights of recovery

standard and labelled surrogate added to the sample, and

RRFsi,rs was the mean relative response factor of the labelled surrogate to the

recovery standard as determined by daily runs of the quantification solution and

defined as

Surrogate standard recoveries (reported with each sample result) were required to be

in the range considered acceptable.

REFERENCES:

1. AXYS ANALYTICAL SERVICES LTD. Analytical Method for the Determination Aroclors,

Total PCBs, Chlorinated Pesticides, PCB Congeners, Coplanar PCBs (Non-Ortho-Substituted,

NOS), Toxaphene, Chlorbenzenes. AXYS Method Doc. CL/01 Rev 3 May 12, 1997. Includes

the tissue method CL-T-02/Ver.2 and the sediment method CL-S-01/Ver.2.

si

rs

rs

si

A

A

 x 

W

W
TABLE 1.

SURROGATE AND INTERNAL (RECOVERY) STANDARD USED FOR PCB ANALYSES

SURROGATE STANDARD

(Axys ID CL027A-SUR)

13C12-PCB 101

13C12-PCB 105

13C12-PCB 118

13C12-PCB 180

13C12-PCB 209

INTERNAL (RECOVERY) STANDARD

(Axys ID CL0010A-REC)

13C12-PCB 153
TABLE 2.

IONS MONITORED BY HRGC/LRMS FOR QUANTIFICATION OF PCBs

PCB CONGENER GROUP QUANTITATION

ION

CONFIRMING

ION

Dichlorobiphenyls 222 224

Trichlorobiphenyls 256 258

Tetrachlorobiphenyls 290 292

Pentachlorobiphenyls 326 328, 330

Hexachlorobiphenyls 360 362

Heptachlorobiphenyls 394 396

Octachlorobiphenyls 428 430

Nonachlorobiphenyls 462 464

Decachlorobiphenyl 498 500
APPENDIX J

Taxonomic Methods
1

LABORATORY TAXONOMIC METHODS

EcoAnalysts, Inc. used the methods described below to process the macroinvertebrate

samples from this project. Included are lists of the equipment and some of the taxonomic

keys employed.

We received the samples, inventoried them, and compared our findings with the chain-ofcustody forms enclosed with the samples. After assuring we had all the samples, we

entered the sample identification information into our computerized tracking system. A

technician re-screened the samples to remove the formalin solution in which the samples

were originally preserved. The technician returned the samples to their original containers

and filled them with 70% ethanol containing 1.5 grams/gallon of Rose Bengal dye. We

let the samples stand for three days, agitating each sample twice a day to ensure the stain

was well distributed.

After staining was complete, our technicians sorted the samples. The protocol provided

by EVS Environment Consultants (EVS) permitted count-based subsampling if necessary,

with a target count of 500 organisms and a 95% sorting efficacy. If a sample was

subsampled, the following procedures were used. The material from each sample was

evenly distributed throughout a gridded (30, 6x6 cm squares) Caton (1991) subsampler.

A square was chosen at random and the material removed from the tray and placed in a

glass petri dish. Using dissecting stereomicroscopes, technicians removed all the

invertebrates from the material. Each square was sorted completely, even if the technician

reached the target count “mid-square.” The technician returned the sorted material to the

original sample container. Another square was selected and the process repeated until the

sorter reached the target count or completely sorted the sample. Technicians placed the

sorted invertebrates in plastic vials, covered them with 70% ethanol, and inserted

appropriate labels. On the sample label and on the associated sorting record sheets, the

technician recorded the number of individuals removed.

After the initial sort was complete, the sample material (or sorted portion thereof) was

redistributed (by a different technician) in a Caton tray for the quality control (QC) sort.

The new technician resorted six squares (20% of the area) using the method described.

Any invertebrates removed during this sort were added to the total from the first sort and

this grand total was divided into the QC resort total to calculate a percent efficacy. If the

QC sort exceeded 5% of the grand total, the technician resorted the entire sample and the

QC process was repeated until the sample passed. We recorded all pertinent information

on a QC sheet and delivered it to EVS.

Once samples were satisfactorily sorted, they were identified by our staff taxonomists.

The macroinvertebrates were removed from the sorting vials, sorted morphologically,
2

enumerated, and identified. EVS specified that Insecta be identified to the lowest

practical level (genus and species) including Chironomidae to genus, and Oligochata

(aquatic worms) to family. Other non-insect taxa were taken to the lowest practical level.

The keys used in making the identifications are listed below. In addition, our taxonomists

referred to our specimen reference collection, most of which have been verified by

recognized experts. Questionable identifications were examined by all of our

taxonomists. Damaged or immature specimens were identified to the lowest possible

level and noted, as were any pupae encountered. Our midge specialist identified

Chrionomidae specimens under a compound scope using temporary wet mounts.

Oligochaetes were mounted on glass slides in CMCP-10, dried, and identified with a

compound microscope. We compiled a synoptic collection of the taxa identified in the

project, which was reviewed by all of our taxonomists upon completion of the project.

As they were determined, identifications and enumerations were recorded and entered into

a desktop computer using our customized entry program. The data were associated with

their respective sample identifiers, compiled, formatted, and delivered to EVS.

Equipment/supplies used:

• Zeiss Stemi 2000 dissecting microscope

• Fiber optic light source

• Caton subsamplers

• Zeiss Axiolab phase contrast compound microscope with turret condenser

• 70% ethanol for preservative

• Tally counters

• Water/alcohol-proof pens

• Label paper

• Miscellaneous forceps, petri dishes, probes, etc.

• Taxonomic keys

• Specimen reference collection, representing over 700 taxa

• Archive-quality laser printed taxa and determination labels

• Desktop computer

Taxonomic Keys:

This list includes specifically those keys used on this particular project. Our library

includes more than 700 individual articles, papers, and handbooks we use address

taxonomy at the genus and species level.

Bergman, E. A. and W. L. Hilsenhoff, 1978. Baetis (Ephemeroptera: Baetidae) of

Wisconsin. The Great Lake Entomologist. Vol. 11(3): 125-135
3

Bode, R.W., 1983. Larvae of North American Eukiefferiella and Tvetenia

(Diptera:Chironomidae). Bulletin of the N.Y. State Museum

Brown, Harley P. 1976. Aquatic Dryopoid Beetles (Coleoptera) of the United States. US

Environmental Protection Agency, Water Pollution Control Series 18050

ELD04/72

Burch, J.B., 1972. Freshwater sphaeriacian clams (Mollusca:Pelecypoda) of North

America. Biota of Freshwater Ecosystems Identification Manual No.3

Burch, J.B., 1973. Freshwater unionacean clams (Mollusca:Pelecypoda) of North

America. Biota of Freshwater Ecosystems Identification Manual No.11

Burch, J.B., 1982. Freshwater snails (Mollusca:Gastopoda) of North America. EPA600/3-82-026.

Burks, D.B., 1953. The Mayflies, or Ephemeroptera of Illinois. Bull. of the Illinois

Natural History Survey

Epler, J.H., 1987. Revision of the Nearctic Dicrotendipes Keiffer, 1913

(Diptera:Chironomidae). Evolutionary Monographs

Grodhaus, G., 1987. Endochironomus Keiffer, Tribelos Townes, Synendotendipes, n.

gen., and Endotribelos, n. gen. (Diptera:Chironomidae) of the Nearctic region..

Journal of the Kansas Entomological Society

LeSage, L., A.D. Harrison, 1980. Taxonomy of Cricotopus species

(Diptera:Chironomidae) from Salem Creek, Ontario. Proceeds of the

Entomological Society of Ontario

McAlpine, J.R., et. al. 1981. Manual of Nearctic Diptera, Volume I. Research Branch

Agriculture Canada. Mongraph No. 27, 674 pp.

Merritt, R. W. and K.W. Cummins, 1996. An introduction to the Aquatic Insects of

North America. 3rd Edition, Kendall/Hunt Publishing Co., Dubuque, Iowa.

Morihara, D.K. and W.P McCafferty, 1979. The Baetis larvae of North America

(Ephemeroptera: Baetidae), Transactions of the American Entomological Society,

Vol. 105:139-221.

Peckarsky, Barbara L. et al, 1990. Freshwater Macroinvertebrates of Northeastern North

America. Cornell University Press, Ithaca, New York.
4

Pennak, Robert W., 1989. Freshwater Invertebrates of the United States, 3rd Edition.

John Wiley & Sons, Inc.

Roback, S.S., 1971. The subfamily Tanypodinae in North America. Monographs of the

Acadamy of Natural Sciences Philadelphia 17:1-140

Saether, O.A., 1975. Nearctic and Palaearctic Heterotrissocladius (

Diptera:Chironomidae). Bulletin of the Fisheries Resource Board of Canada.

Bulletin 193.

Saether, O.A., 1976. Revision of Hydrobaenus, Trissocladius, Zalutschia,

Paratrissocladius, and some related genera.. Bulletin of the Fisheries Resource

Board of Canada. Bulletin 195.

Saether, O.A., 1977. Taxonomic studies on Chironomidae: Nanocladius,

Pseudochironomus, and the Harnischia complex.. Bulletin of the Fisheries

Resource Board of Canada. Bulletin 196.

Simpson, K.W., and K.W. Bode, 1980. Common larvae of Chironomidae (Diptera)

from New York State streams and rivers with particular reference to the fauna of

artificial substrates. New York State Museum Bulletin No. 439.

Stewart, K.W., B.P. Stark, 1993. Nymphs of the North American Stonefly Genera

(Plecoptera). University of North Texas Press, Denton, Texas.

Wang, T.Q., W.P. McCafferty 1996. New diagnostic characters for the mayfly family

Baetidae (Ephemeroptera). Entomological News

Wiederholm, T., 1983. The larvae of Chironomidae (Diptera) of the Holarctic Region.

Part 1: Larvae. Ent. Scand. Suppl. No 19.

Wiederholm, T., 1986. Chironomidae of the Holarctic Region. Part 2: Pupae. Ent. Scand.

Suppl. No. 28.

Wiggins, Glenn B., 1996. Larvae of the North American Caddisfly Genera (Trichoptera),

2nd Edition. University of Toronto Press, Toronto, Canada.

Literature Cited:

Caton, L.W. 1991. Improved Subsampling Methods for the EPA “Rapid Bioassessment”

Benthic Protocols. Bulletin the the North American Benthological Society. Vol.

8(3):317-319.
5

Merritt, R. W. and K.W. Cummins, 1996. An introduction to the Aquatic Insects of

North America. 3rd Edition, Kendall/Hunt Publishing Co., Dubuque, Iowa.

Waltz, R.D. and W.P. McCafferty, 1990. Revisionary synopsis of the Baetidae

(Ephemeroptera) of North and Middle America. Transactions fo the American

Entomological Society. 116(4):769-799.

Waltz, R.D., G.F. Edmonds, Jr. and G. Lester, 1998. Scientific Note; New distributions

of Raptoheptagenia cruentata and Ametropus neavei (Ephemeroptera:

Heptageniidae, Ametropodidae). Ent. News. 109(3):213-214.