Exhibit 31.1

              CERTIFICATION OF CHIEF OPERATING OFFICER PURSUANT TO
                  SECTION 302 OF THE SARBANES-OXLEY ACT OF 2002

I, J. Richard George, certify that:

1. I have  reviewed  this  annual  report on Form  10-KSB/A  (No.  2) of Calypte
Biomedical Corporation;

2. Based on my knowledge, this report does not contain any untrue statement of a
material fact or omit to state a material fact  necessary to make the statements
made, in light of the  circumstances  under which such statements were made, not
misleading with respect to the period covered by this report;

3.  Based  on my  knowledge,  the  financial  statements,  and  other  financial
information included in this report, fairly present in all material respects the
financial condition,  results of operations and cash flows of the small business
issuer as of, and for, the periods presented in this annual report;

4. The small business issuer's other certifying officer(s) and I are responsible
for establishing and maintaining  disclosure controls and procedures (as defined
in Exchange  Act Rules  13a-15(e)  and 15d -15(e)),  and  internal  control over
financial  reporting (as defined in Exchange Act Rules  13a-15(f) and 15d-15(f))
for the small business issuer and have:

      (a)  Designed  such  disclosure  controls and  procedures,  or caused such
      disclosure  controls and procedures to be designed under our  supervision,
      to ensure that material information relating to the small business issuer,
      including  its  consolidated  subsidiaries,  is made known to us by others
      within those entities, particularly during the period in which this annual
      report is being prepared;

      (b) Designed such internal  control over  financial  reporting,  or caused
      such internal  control over  financial  reporting to be designed under our
      supervision,  to provide reasonable assurance regarding the reliability of
      financial  reporting  and the  preparation  of  financial  statements  for
      external  purposes  in  accordance  with  generally  accepted   accounting
      principles;

      (c) Evaluated the effectiveness of the small business issuer's  disclosure
      controls and  procedures,  and  presented  in this report our  conclusions
      about the effectiveness of the disclosure  controls and procedures,  as of
      the end of the period covered by this report based on such evaluation; and

      (d)  Disclosed  in this report any change in the small  business  issuer's
      internal  control over financial  reporting that occurred during the small
      business  issuer's most recent fiscal quarter (the small business issuer's
      fourth fiscal quarter in the case of an annual report) that has materially
      affected, or is reasonably likely to materially affect, the small business
      issuer's internal control over financial reporting; and;

5. The small business issuer's other certifying officer(s) and I have disclosed,
based on our most recent evaluation, to the small business issuer's auditors and
the audit  committee  of the small  business  issuer's  board of  directors  (or
persons performing the equivalent function):

      (a) all significant  deficiencies and material weaknesses in the design or
      operation  of  internal  controls  over  financial   reporting  which  are
      reasonably  likely to adversely affect the small business issuer's ability
      to record, process, summarize and report financial information; and

      (b) any fraud, whether or not material,  that involves management or other
      employees  who have a  significant  role in the  small  business  issuer's
      internal controls over financial reporting.


Date: June 27, 2005

/s/ J. Richard George
---------------------
J. Richard George
President and Chief Executive Officer