Initial to indicate certification as to information on this page (full text of certification is on page one) 

13. Phone Number

10. Certification: I certify that the statements made on this form and all attachments are true, accurate, and complete. I acknowledge that any 

knowingly false or misleading statement may be punishable by fine, imprisonment or both under applicable law.

12. Name of Company

11. Date

OMB Approval 2070-0174 

EPA FORM 6300-3

United States Environmental Protection Agency 

Washington, D.C. 20460

REQUIREMENTS STATUS AND REGISTRANT'S RESPONSE

INSTRUCTIONS: Please type or print in ink. Please read carefully the attached instructions and supply the information requested on this form. Use additional sheet(s) if necessary.

4. Guideline

Requirement

Number

5. Study Title Progress 

Reports

1 2 3

6. Use 

Pattern

7. Test 

Substance

8. Time

Frame 

(Months)

9. Registrant

Response

P

R

O

T

O

C

O

L

1. Company Name and Address 2. Case # and Name 3. Date and Type of DCI and Number

Signature and Title of Company's Authorized Representative _________________________________________________________________

GOWAN COMPANY

P.O. Box 5569

YUMA, AZ 85366 

Environmental Fate Data Requirements (Conventional 

Chemical)

Nontarget Plant Protection Data Requirements (Conventional 

Chemical)

Terrestrial and Aquatic Nontarget Organisms Data 

Requirements (Conventional Chemical)

835.4100

835.4400

850.4100

850.1735

850.1740

850.2100

Aerobic soil metabolism

Anaerobic aquatic metabolism

Seedling Emergence and Seedling Growth

Whole sediment: acute freshwater invertebrates

Whole sediment: acute marine invertebrates

Avian acute oral toxicity test

N/A - Zoxamide

Chemical # and Name: 101702

Zoxamide

GENERIC

ID # GDCI-101702-1473

A

A

A

A

A

A

COMMENT

COMMENT

TEP

TGAI

TGAI

TGAI

24

24

12

12

12

12

Page 1 of 

(2, 14)

(2, 15)

(13)

(16)

(17)

(18, 20)

N

N

N

N

N

Y

2
Date

OMB Approval 2070-0174 

EPA FORM 6300-3

United States Environmental Protection Agency 

Washington, D.C. 20460

REQUIREMENTS STATUS AND REGISTRANT'S RESPONSE

INSTRUCTIONS: Please type or print in ink. Please read carefully the attached instructions and supply the information requested on this form. Use additional sheet(s) if necessary.

4. Guideline

Requirement

Number

5. Study Title Progress 

Reports

1 2 3

6. Use 

Pattern

7. Test 

Substance

8. Time

Frame 

(Months)

9. Registrant

Response

P

R

O

T

O

C

O

L

1. Company Name and Address 2. Case # and Name 3. Date and Type of DCI and Number

GOWAN COMPANY

P.O. Box 5569

YUMA, AZ 85366 

850.3040

SS-1155

SS-1312

SS-1313

SS-1314

SS-1342

Field testing for pollinators

Residues in Pollen and Nectar/Field Residue Analysis

Honey bee larvae acute oral toxicity

Honey bee adult chronic oral toxicity

Honey bee larvae chronic oral toxicity

Semi Field Testing for Pollinators (Tunnel or Colony 

Feeding Studies)

N/A - Zoxamide

Chemical # and Name: 101702

Zoxamide

GENERIC

ID # GDCI-101702-1473

A

A

A

A

A

A

TEP

TEP

TGAI

TGAI

TGAI

TEP

24

24

12

12

12

24

Page 2 of 

(1, 4, 5, 20)

(9, 19, 20)

(8, 10, 20)

(6, 20)

(7, 10, 20)

(3, 11, 12, 

20)

Y

Y

Y

Y

Y

Y

2
United States Environmental Protection Agency 

Washington, D.C. 20460

FOOTNOTES AND KEY DEFINITIONS FOR GUIDELINE REQUIREMENTS

N/A - Zoxamide

GDCI-101702-1473

1

2

3

4

5

6

7

8

9

10

11

12

13

Key: Residue of Concern = Residue of Concern; TEP = Typical End Use Product [TEP]; TGAI = Technical Grade Active Ingredient [TGAI]; TGAI/PAIRA = Technical Grade of the Active Ingredient or Pure Active 

Ingredient, Radio Labelled; TGAI, TEP = Technical Grade of the Active Ingredient or Technical End-Use Product; TGAI/PAI = Technical Grade Active Ingredient, Pure Active Ingredient

USEPA. 2012c. Field Testing for Pollinators. Ecological Effects Test Guidelines OCSPP 850.3040. EPA 712-C-017. Web. http://www.regulations.gov/#!documentDetail;D=EPA-HQ-OPPT-2009-

0154-0018

The test material should be technical grade or a pure active ingredient radiolabelled.

The need for a semi-field test for pollinators (i.e., either a field-feeding test or a tunnel test) will be determined based upon lower-tiered tests and/or other lines of evidence, and the need for a 

refined pollinator risk assessment.

The need for a field test for pollinators will be determined based upon lower-tiered tests and/or other lines of data and the need for a refined pollinator risk assessment.

See information and guidance identified in the EPA documents, (i) USEPA. 2012. White Paper in Support of the Proposed Risk Assessment Process for Bees. Submitted to the FIFRA Scientific 

Advisory Panel for Review and Comment September 11 ¿ 14, 2012. Office of Chemical Safety and Pollution Prevention Office of Pesticide Programs Environmental Fate and Effects Division, 

Environmental Protection Agency, Washington DC; Environmental Assessment Directorate, Pest Management Regulatory Agency, Health Canada, Ottawa, CN; California Department of Pesticide 

Regulation http://www.regulations.gov/#!documentDetail;D=EPA-HQ-OPP-2012-0543-0004; (ii) 2014 Guidance for Assessing Pesticide Risks to Bees. Office of Pesticide Programs United States 

Environmental Protection Agency, Health Canada Pest Management Regulatory Agency, California Department of Pesticide Regulation. June 19, 2014. 

http://www2.epa.gov/sites/production/files/2014-06/documents/pollinator_risk_assessment_guidance_06_19_14.pdf

OECD has not yet finalized test guidelines for chronic studies, and efforts are underway to develop standardized guidelines for assessing the effects from chronic exposure to adult and larvae 

in the laboratory. Discussion of the study design elements for the 10-day adult toxicity test can be found in Appendix O of the European Food Safety Authority (EFSA) guidance document: 

EFSA. Guidance on the risk assessment of plant protection products on bees (Apis mellifera, Bombus spp. and solitary bees. EFSA Journal 2013;11(7):3295, 266 pp. 

doi:10.2903/j.efsa.2013.3295. 

Available online at: http://www.efsa.europa.eu/en/efsajournal/doc/3295.pdf

OECD has not yet finalized test guidelines for chronic studies with honey bee larvae. OECD draft guidance has is being developed, see OECD 2013b. OECD Draft Guidance Document Honey Bee

(Apis mellifera) Larval Toxicity Test, Repeated Exposure. http://www.oecd.org/env/ehs/testing/Draft_GD_honeybees_rep_exp_for_2nd_CR_25_November_2013.pdf

OECD Test Guideline 237 may be used to develop a protocol for this study (OECD. 2013 Guidelines for Testing Chemicals. Honey bee (Apis mellifera) larval toxicity test, single exposure.) See: 

http://www.oecd-ilibrary.org/environment/test-no-237-honey-bee-apis-mellifera-larval-toxicity-test-single-exposure_9789264203723-en

Measurements of residues in the pollen/nectar are needed based upon lower-tiered tests and/or other lines of evidence, and the need for a refined pollinator risk assessment.

In some cases, information pertaining to acute toxicity to honey bee larvae may be obtained with the chronic honey bee larvae test thereby negating the need for separate acute and chronic 

larval toxicity tests.

Formal guidelines for semi-field tests do not yet exist; however, information that can help guide the development of either a semi-field tunnel test protocol can be found at OECD 75, see: OECD. 

2007. Series on Testing and Assessment Number 75. Guidance document on the honey bee (Apis mellifera L.) brood test under semi-field conditions. Environmental Directorate Joint Meeting of 

the Chemicals Committee and the Working Party on Chemicals, Pesticides and Biotechnology. ENV/JM/MONO(2007)22. 31-Aug-2007. 

http://www.oecd.org/officialdocuments/publicdisplaydocumentpdf/?cote=env/jm/mono(2007)22&doclanguage=en.

For field-feeding studies see: Oomen et al. 1992: Oomen, P. A. A. DeRuijter and J. Van der Steen. 1992. Method for honey bee brood feeding tests with insect growth-regulating insecticides. 

Bul OEPP/EPPO Bulletin 22: 613 ¿ 616.

Data are required on two species: corn and ryegrass. For corn, a study that defines a NOAEC is required. For ryegrass, a Tier II study is required.

Case # and Name:

DCI Number:

Page: 1 of

Footnotes: The following footnotes are referenced in column two (5. Study Title) of the Requirements Status and Registrant's Response form. 

These footnotes apply in addition to any test notes included in 40 CFR Part 158 with respect to the particular data requirement.

Use Categories Key:

A - Terrestrial food crop

2
United States Environmental Protection Agency 

Washington, D.C. 20460

FOOTNOTES AND KEY DEFINITIONS FOR GUIDELINE REQUIREMENTS

N/A - Zoxamide

GDCI-101702-1473

14

15

16

17

18

19

20

Key: Residue of Concern = Residue of Concern; TEP = Typical End Use Product [TEP]; TGAI = Technical Grade Active Ingredient [TGAI]; TGAI/PAIRA = Technical Grade of the Active Ingredient or Pure Active 

Ingredient, Radio Labelled; TGAI, TEP = Technical Grade of the Active Ingredient or Technical End-Use Product; TGAI/PAI = Technical Grade Active Ingredient, Pure Active Ingredient

Data are required on two soils.

Data are required on two sediments.

Data are required for the midge Chironomus dilutus and freshwater amphipod Hyalella azteca.

Data are required for the estuarine/marine amphipod Leptocheirus plumulosus.

An avian oral toxicity study is required for a passerine species.

A study protocol must be submitted to, and reviewed by EPA, prior to study initiation. The following elements could be considered when developing study protocol(s) for the monitoring of 

residues in pollen/nectar: (1) Consideration of the range of application methods and environmental conditions (e.g., soil and hydric regimes) that the target crop(s) may be under; (2) 

Consideration of the attractiveness of the selected crop to pollinators; (3) Consideration of a collection schedule sufficient to allow for an understanding of the character of residues, in the 

pollen/nectar and/or plant tissues, over time; (4) Consideration of data sufficient to determine whether residues of the active ingredient and/or degradation product(s) accumulates in soil and 

is/are bioavailable for plant to uptake in a following planting, and therefore result in potential exposure to pollinators; (5) Consideration of the market proportion of the selected target crop(s).

A protocol must be submitted for EPA review within 90 days of receipt of the Data Call-In and prior to study initiation.

Case # and Name:

DCI Number:

Page: 2 of 2