57908 Federal Register / Vol. 74, No. 216 / Tuesday, November 10, 2009 / Rules and Regulations 

IV. Statutory and Executive Order 

Reviews 

Under the Clean Air Act, the 

Administrator is required to approve a 

SIP submission that complies with the 

provisions of the Act and applicable 

Federal regulations. 42 U.S.C. 7410(k); 

40 CFR 52.02(a). Thus, in reviewing SIP 

submissions, EPA’s role is to approve 

State choices, provided that they meet 

the criteria of the Clean Air Act. 

Accordingly, this action merely 

approves State law as meeting Federal 

requirements and does not impose 

additional requirements beyond those 

imposed by State law. For that reason, 

this action: 

• Is not a ‘‘significant regulatory 

action’’ subject to review by the Office 

of Management and Budget under 

Executive Order 12866 (58 FR 51735, 

October 4, 1993); 

• Does not impose an information 

collection burden under the provisions 

of the Paperwork Reduction Act (44 

U.S.C. 3501 et seq.); 

• Is certified as not having a 

significant economic impact on a 

substantial number of small entities 

under the Regulatory Flexibility Act (5 

U.S.C. 601 et seq.); 

• Does not contain any unfunded 

mandate or significantly or uniquely 

affect small governments, as described 

in the Unfunded Mandates Reform Act 

of 1995 (Pub. L. 104–4); 

• Does not have Federalism 

implications as specified in Executive 

Order 13132 (64 FR 43255, August 10, 

1999); 

• Is not an economically significant 

regulatory action based on health or 

safety risks subject to Executive Order 

13045 (62 FR 19885, April 23, 1997); 

• Is not a significant regulatory action 

subject to Executive Order 13211 (66 FR 

28355, May 22, 2001); 

• Is not subject to requirements of 

Section 12(d) of the National 

Technology Transfer and Advancement 

Act of 1995 (15 U.S.C. 272 note) because 

application of those requirements would 

be inconsistent with the Clean Air Act; 

and 

• Does not provide EPA with the 

discretionary authority to address, as 

appropriate, disproportionate human 

health or environmental effects, using 

practicable and legally permissible 

methods, under Executive Order 12898 

(59 FR 7629, February 16, 1994). 

In addition, this rule does not have 

tribal implications as specified by 

Executive Order 13175 (65 FR 67249, 

November 9, 2000), because the SIP is 

not approved to apply in Indian country 

located in the State, and EPA notes that 

it will not impose substantial direct 

costs on tribal governments or preempt 

tribal law. 

The Congressional Review Act, 5 

U.S.C. 801 et seq., as added by the Small 

Business Regulatory Enforcement 

Fairness Act of 1996, generally provides 

that before a rule may take effect, the 

agency promulgating the rule must 

submit a rule report, which includes a 

copy of the rule, to each House of the 

Congress and to the Comptroller General 

of the United States. EPA will submit a 

report containing this action and other 

required information to the U.S. Senate, 

the U.S. House of Representatives, and 

the Comptroller General of the United 

States prior to publication of the rule in 

the Federal Register. A major rule 

cannot take effect until 60 days after it 

is published in the Federal Register. 

This action is not a ‘‘major rule’’ as 

defined by 5 U.S.C. 804(2). 

Under section 307(b)(1) of the Clean 

Air Act, petitions for judicial review of 

this action must be filed in the United 

States Court of Appeals for the 

appropriate circuit by January 11, 2010. 

Filing a petition for reconsideration by 

the Administrator of this final rule does 

not affect the finality of this action for 

the purposes of judicial review nor does 

it extend the time within which a 

petition for judicial review may be filed, 

and shall not postpone the effectiveness 

of such rule or action. This action may 

not be challenged later in proceedings to 

enforce its requirements (see section 

307(b)(2)). 

List of Subjects in 40 CFR Part 52 

Environmental protection, Air 

pollution control, Incorporation by 

reference, Intergovernmental relations, 

Particulate matter, Reporting and 

recordkeeping requirements. 

Dated: September 18, 2009. 

Jane Diamond, 

Acting Regional Administrator, Region IX. 

■ Part 52, Chapter I, Title 40 of the Code 

of Federal Regulations is amended as 

follows: 

PART 52—[AMENDED] 

■ 1. The authority citation for Part 52 

continues to read as follows: 

Authority: 42 U.S.C. 7401 et seq. 

Subpart F—California 

■ 2. Section 52.220 is amended by 

adding paragraphs (c)(364)(i)(A)(2) and 

(c)(364)(i)(B), and (c)(366) to read as 

follows: 

§ 52.220 Identification of plan. 

* * * * * 

(c) * * * 

(364) * * * 

(i) * * * 

(A) * * * 

(2) Rule 4901, ‘‘Wood Burning 

Fireplaces and Wood Burning Heaters,’’ 

amended on October 16, 2008. 

* * * * * 

(B) South Coast Air Quality 

Management District. 

(1) Rule 1158, ‘‘Storage, Handling, 

and Transport of Coke, Coal and 

Sulfur,’’ amended July 11, 2008. 

* * * * * 

(366) New and amended regulations 

for the following agencies were 

submitted on April 6, 2009, by the 

Governor’s designee. 

(i) Incorporation by reference. 

(A) San Joaquin Valley Unified Air 

Pollution Control District. 

(1) Rule 4103, ‘‘Open Burning,’’ 

amended May 17, 2007. 

* * * * * 

[FR Doc. E9–26958 Filed 11–9–09; 8:45 am] 

BILLING CODE 6560–50–P 

ENVIRONMENTAL PROTECTION 

AGENCY 

40 CFR Part 141 

[EPA–HQ–OW–2009–0707; FRL–8979–5] 

Expedited Approval of Alternative Test 

Procedures for the Analysis of 

Contaminants Under the Safe Drinking 

Water Act; Analysis and Sampling 

Procedures 

AGENCY: Environmental Protection 

Agency (EPA). 

ACTION: Final rule. 

SUMMARY: This action announces the 

Environmental Protection Agency’s 

(EPA’s) approval of alternative testing 

methods for use in measuring the levels 

of contaminants in drinking water and 

determining compliance with national 

primary drinking water regulations. The 

Safe Drinking Water Act (SDWA) 

authorizes EPA to approve the use of 

alternative testing methods through 

publication in the Federal Register. EPA 

is using this streamlined authority to 

make 25 additional methods available 

for analyzing drinking water samples 

required by regulation. This expedited 

approach provides public water 

systems, laboratories, and primacy 

agencies with more timely access to new 

measurement techniques and greater 

flexibility in the selection of analytical 

methods, thereby reducing monitoring 

costs while maintaining public health 

protection. 

DATES: This action is effective 

November 10, 2009. 

FOR FURTHER INFORMATION CONTACT: Safe 

Drinking Water Hotline: (800) 426–4791 


srobinson on DSKHWCL6B1PROD with RULES
Federal Register / Vol. 74, No. 216 / Tuesday, November 10, 2009 / Rules and Regulations 57909 

or Patricia Snyder Fair, Technical 

Support Center, Office of Ground Water 

and Drinking Water (MS 140), 

Environmental Protection Agency, 26 

West Martin Luther King Drive, 

Cincinnati, OH 45268; telephone 

number: (513) 569–7937; e-mail address: 

fair.pat@epa.gov. 

SUPPLEMENTARY INFORMATION: 

I. General Information 

A. Does This Action Apply to Me? 

Public water systems are the regulated 

entities required to measure 

contaminants in drinking water 

samples. In addition, EPA Regions as 

well as States and Tribal governments 

with authority to administer the 

regulatory program for public water 

systems under SDWA may also measure 

contaminants in water samples. When 

EPA sets a monitoring requirement in its 

national primary drinking water 

regulations for a given contaminant, the 

Agency also establishes in the 

regulations standardized test procedures 

for analysis of the contaminant. This 

action makes alternative testing 

methods available for particular 

drinking water contaminants beyond the 

testing methods currently established in 

the regulations. EPA is providing public 

water systems required to test water 

samples with a choice of using either a 

test procedure already established in the 

existing regulations or an alternative test 

procedure that has been approved in 

this action. Categories and entities that 

may ultimately be affected by this action 

include: 

Category Examples of potentially regulated entities NAICS1

State, Local, & Tribal Governments ....... States, local and Tribal governments that analyze water samples on behalf of public 

water systems required to conduct such analysis; States, local and Tribal governments that themselves operate community and non-transient non-community water 

systems required to monitor.

924110 

Industry ................................................... Private operators of community and non-transient non-community water systems required to monitor.221310 

Municipalities .......................................... Municipal operators of community and non-transient non-community water systems 

required to monitor.

924110 

1 North American Industry Classification System. 

This table is not exhaustive, but rather 

provides a guide for readers regarding 

entities likely to be affected by this 

action. This table lists the types of 

entities that EPA is now aware could 

potentially be affected by this action. 

Other types of entities not listed in the 

table could also be impacted. To 

determine whether your facility is 

affected by this action, you should 

carefully examine the applicability 

language at 40 CFR 141.2 (definition of 

public water system). If you have 

questions regarding the applicability of 

this action to a particular entity, consult 

the person listed in the preceding FOR

FURTHER INFORMATION CONTACT section. 

B. How Can I Get Copies of This 

Document and Other Related 

Information? 

Docket. EPA established a docket for 

this action under Docket ID No. EPA– 

HQ–OW–2009–0707. Publicly available 

docket materials are available either 

electronically through http:// 

www.regulations.gov or in hard copy at 

the Water Docket in the EPA Docket 

Center, (EPA/DC) EPA West, Room 

3334, 1301 Constitution Ave., NW., 

Washington, DC. Copyrighted materials 

are available only in hard copy. The 

EPA Docket Center Public Reading 

Room is open from 8:30 a.m. to 4:30 

p.m., Monday through Friday, excluding 

legal holidays. The telephone number 

for the Public Reading Room is (202) 

566–1744, and the telephone number for 

the Water Docket is (202) 566–2426. 

Abbreviations and Acronyms Used in 

This Action 

CFR: Code of Federal Regulations. 

DOC: Dissolved Organic Carbon. 

DPD: N,N-Diethyl-p-phenylenediamine. 

E. coli: Escherichia coli. 

EPA: Environmental Protection Agency. 

HAA5: Haloacetic Acids (five); Sum of 

Monochloroacetic Acid, Dichloroacetic 

Acid, Trichloroacetic Acid, 

Monobromoacetic Acid, and Dibromoacetic 

Acid. 

IC: Ion Chromatography. 

IC–ESI–MS/MS: Ion Chromatography 

Electrospray Ionization Tandem Mass 

Spectrometry. 

LED: Light Emitting Diode. 

mg/L: Milligrams/Liter. 

MRL: Minimum Reporting Level. 

NEMI: National Environmental Methods 

Index. 

nm: Nanometers. 

QC: Quality Control. 

SDWA: Safe Drinking Water Act. 

SUVA: Specific Ultraviolet Absorbance. 

TOC: Total Organic Carbon. 

UV254: Ultraviolet Absorbance at 254 

nanometers. 

Table of Contents 

I. General Information 

A. Does This Action Apply to Me? 

B. How Can I Get Copies of This Document 

and Other Related Information? 

II. Background 

A. What Is the Purpose of This Action? 

B. What Is the Basis for This Action? 

III. Summary of Approvals 

A. Methods Developed by EPA 

B. Methods Developed by Voluntary 

Consensus Standard Bodies (VCSB) 

C. Methods Developed by Vendors 

IV. Statutory and Executive Order Reviews 

V. References 

II. Background 

A. What Is the Purpose of This Action? 

In this action, EPA is approving 25 

analytical methods for determining 

contaminant concentrations in samples 

collected under SDWA. Regulated 

parties required to sample and monitor 

may use either the testing methods 

already established in existing 

regulations or the alternative testing 

methods being approved in this action. 

The new methods are listed in 

Appendix A to Subpart C in 40 CFR 141 

and on EPA’s drinking water methods 

Web site at http://www.epa.gov/ 

safewater/methods/ 

analyticalmethods_expedited.html. 

B. What Is the Basis for This Action? 

When EPA determines that an 

alternative analytical method is 

‘‘equally effective’’ (i.e., as effective as a 

method that has already been 

promulgated in the regulations), SDWA 

allows EPA to approve the use of the 

alternative method through publication 

in the Federal Register. (See Section 

1401(1) of SDWA.) EPA is using this 

streamlined approval authority to make 

25 additional methods available for 

determining contaminant 

concentrations in samples collected 

under SDWA. EPA has determined that, 

for each contaminant or group of 

contaminants listed in Section III, the 

additional testing methods being 

approved in this action are equally 

effective as one or more of the testing 

methods already established in the 

regulations for those contaminants. 


srobinson on DSKHWCL6B1PROD with RULES
57910 Federal Register / Vol. 74, No. 216 / Tuesday, November 10, 2009 / Rules and Regulations 

Section 1401(1) states that the newly 

approved methods ‘‘shall be treated as 

an alternative for public water systems 

to the quality control and testing 

procedures listed in the regulation.’’ 

Accordingly, this action makes these 

additional (and optional) 25 analytical 

methods legally available for meeting 

EPA’s monitoring requirements. 

This action does not add regulatory 

language, but does, for informational 

purposes, update an appendix to the 

regulations at 40 CFR part 141 that lists 

all methods approved under Section 

1401(1) of SDWA. Accordingly, while 

this action is not a rule, it is updating 

CFR text and therefore is being 

published in the ‘‘Final Rules’’ section 

of this Federal Register. 

EPA described this expedited 

methods approval process in an April 

10, 2007, Federal Register notice (72 FR 

17902) (USEPA 2007) and announced 

its intent to begin using the process. 

EPA published the first set of approvals 

in a June 3, 2008, Federal Register 

notice (73 FR 31616) (USEPA 2008) and 

added Appendix A to 40 CFR Part 141, 

Subpart C. Six additional methods were 

added to Appendix A to Subpart C in 

an August 3, 2009, Federal Register 

notice (74 FR 38348) (USEPA 2009a). 

Future approvals using this process are 

anticipated. 

III. Summary of Approvals 

EPA is approving 25 methods that are 

equally effective relative to methods 

previously promulgated in the 

regulations. By means of this notice, 

these 25 methods are added to 

Appendix A of 40 CFR Part 141, Subpart 

C. 

A. Methods Developed by EPA 

1. EPA Method 334.0, ‘‘Determination 

of Residual Chlorine in Drinking Water 

Using an On-line Chlorine Analyzer’’ 

(USEPA 2009b) establishes quality 

control (QC) criteria for on-line chlorine 

analyzers such that the analyzers 

provide data equivalent to the grab 

sample methodologies that are already 

approved in the regulations. The on-line 

chlorine analyzer is calibrated using 

aqueous standards or the results from 

grab samples that are collected at the 

same sample point as used by the 

analyzer. The grab samples are analyzed 

for chlorine using a method that is 

approved for drinking water compliance 

monitoring. The accuracy of the on-line 

chlorine analyzer is periodically 

verified (and adjustments made when 

necessary) based on results from grab 

sample analyses. 

Previously approved methods for 

determining free and total chlorine 

residuals in drinking water are listed in 

the tables at 40 CFR 141.74(a)(2) and 40 

CFR 141.131(c)(1). All of the methods 

are designed for grab sample analyses. 

The regulation at 40 CFR 141.74(a)(2) 

also states, ‘‘Free and total chlorine 

residuals may be measured 

continuously by adapting a specified 

chlorine residual method for use with a 

continuous monitoring instrument 

provided the chemistry, accuracy, and 

precision remain the same. Instruments 

used for continuous monitoring must be 

calibrated with a grab sample 

measurement at least every five days, or 

with a protocol approved by the State.’’ 

Continuous monitoring instruments 

that use N,N-Diethyl-pphenylenediamine (DPD) chemistry are 

the only on-line chlorine analyzers that, 

prior to this action, met the drinking 

water regulatory requirement to use the 

same chemistry as an approved method. 

The instruments perform chlorine 

residual measurements on a frequent 

basis using an automated version of 

Standard Method 4500–Cl G (APHA 

1998), which is listed in the tables at 40 

CFR 141.74(a)(2) and 40 CFR 

141.131(c)(1). Since the instruments use 

an approved method, they have the 

capability to provide the same accuracy 

and precision as the approved method 

(Standard Method 4500–Cl G), if they 

are properly installed and maintained. 

The performance characteristics of the 

instruments are periodically checked by 

comparing the instrumental results to 

grab sample measurements according to 

a protocol approved by the State. 

EPA Method 334.0 now allows the 

use of on-line chlorine analyzers based 

on chemistry different from that of 

approved methods. It is a ‘‘performance 

based’’ method, which means it 

establishes QC criteria to bench-mark 

the performance of the on-line chlorine 

analyzer against the performance of 

approved grab sample methods. As long 

as the on-line analyzer meets the QC 

criteria in EPA Method 334.0, the data 

are deemed equivalent to data obtained 

using the approved grab sample 

methods. EPA Method 334.0 can be 

used with any type of on-line chlorine 

analyzer. 

Data from 38 drinking water treatment 

facilities (EE&T, Inc. 2009) were used as 

the basis for establishing the on-line 

chlorine analyzer QC criteria in EPA 

Method 334.0. Chlorine residual 

measurements from on-line 

amperometric chlorine analyzers were 

compared to the results from grab 

sample analyses performed using either 

Standard Method 4500–Cl D 

(amperometric titration) (APHA 1998) or 

Standard Method 4500–Cl G (DPD 

colorimetric). Both Standard Methods 

are approved for drinking water 

compliance monitoring analyses and are 

listed in the tables at 40 CFR 

141.74(a)(2) and 40 CFR 141.131(c)(1). 

The data from the 38 facilities 

demonstrate that on-line amperometric 

chlorine analyzers can provide data that 

are equivalent to approved methods. 

EPA Method 334.0 requires that the 

analyst demonstrate that the grab 

sample method provides reliable data 

prior to using it to verify the 

performance of an on-line chlorine 

analyzer. This QC requirement is 

consistent with the QC requirements in 

the approved grab sample methods. 

Aqueous standards are analyzed to 

demonstrate the accuracy and precision 

of the measurements. EPA recommends 

that the grab sample QC requirements in 

EPA Method 334.0 be used with all online chlorine analyzers, including those 

that are originally approved under the 

provisions of 40 CFR 141.74. 

A preliminary draft of EPA Method 

334.0 was provided to the Association 

of State Drinking Water Administrators, 

the American Water Works Association, 

and the Water and Wastewater 

Equipment Manufacturers Association. 

A revised draft was reviewed by persons 

from two State agencies and two 

drinking water utilities. The final 

method reflects changes made in 

response to review comments. The 

public docket for this action includes 

the comments from these organizations 

and the Agency’s response to comments 

(USEPA 2009c). 

EPA has determined that EPA Method 

334.0 is equally effective for measuring 

free and total chlorine residuals as the 

methods that are promulgated in the 

regulations at 40 CFR 141.74(a)(2) and 

40 CFR 141.131(c)(1). The basis for this 

determination is discussed in Fair and 

Wendelken 2009. EPA is therefore 

approving use of EPA Method 334.0 for 

on-line analyses of free and total 

chlorine. A copy of the method can be 

accessed and downloaded directly online at http://epa.gov/safewater/ 

methods/analyticalmethods_

ogwdw.html. 

2. EPA Method 302.0, ‘‘Determination 

of Bromate in Drinking Waters using 

Two-Dimensional Ion Chromatography 

with Suppressed Conductivity 

Detection’’ (USEPA 2009d) is a large 

volume (1.0 mL), two-dimensional ion 

chromatography (IC) method that uses 

suppressed conductivity detection for 

the determination of bromate in raw and 

finished drinking waters. Because this 

method utilizes two dissimilar IC 

columns it does not require second 

column confirmation. Detection and 

quantitation are accomplished in the 

second dimension by suppressed 

conductivity measurement. Bromate 


srobinson on DSKHWCL6B1PROD with RULES
Federal Register / Vol. 74, No. 216 / Tuesday, November 10, 2009 / Rules and Regulations 57911 

concentration is calculated using the 

integrated peak area and the external 

standard technique. 

EPA Method 302.0 offers increased 

bromate specificity without the 

complexity of post column reactors. 

The approved methods for bromate 

are listed at 40 CFR 141.131(b)(1). The 

performance characteristics of EPA 

Method 302.0 were compared to the 

characteristics of approved EPA 

Methods 300.1 (USEPA 2000), 317.0, 

Revision 2.0 (USEPA 2001), and 326.0 

(USEPA 2002). EPA has determined that 

EPA Method 302.0 is equally effective 

for measuring bromate concentrations as 

these approved methods. EPA Method 

302.0 can also meet the minimum 

reporting limit (MRL) requirements 

necessary for methods that are used to 

support the reduced bromate monitoring 

specified at 40 CFR 141.132(b)(3)(ii)(B). 

The basis for these determinations is 

discussed in Munch 2009a. EPA is 

therefore approving EPA Method 302.0 

for the routine determination of bromate 

in drinking water and also allowing its 

use for reduced bromate monitoring. A 

copy of the method can be accessed and 

downloaded directly on-line at http:// 

epa.gov/safewater/methods/ 

analyticalmethods_ogwdw.html. 

3. EPA Method 557, ‘‘Determination 

of Haloacetic Acids, Bromate, and 

Dalapon in Drinking Water by Ion 

Chromatography Electrospray Ionization 

Tandem Mass Spectrometry (IC–ESI– 

MS/MS)’’ (USEPA 2009e) is a directinjection, ion chromatography, negativeion electrospray ionization, tandem 

mass spectrometry (IC–ESI–MS/MS) 

method for the determination of nine 

haloacetic acids in finished drinking 

waters. Each method analyte is 

qualitatively identified via a unique 

mass transition, and the concentration is 

calculated using the integrated peak 

area and the internal standard 

technique. 

Bromate may be measured 

concurrently with the haloacetic acids. 

Real time, chromatographic separation 

of common anions in drinking water 

(matrix elimination) is a key feature of 

this method. Acceptable method 

performance has been demonstrated for 

matrix ion concentrations of 320 

milligrams/Liter (mg/L) chloride, 250 

mg/L sulfate, 150 mg/L bicarbonate and 

20 mg/L nitrate. 

EPA Method 557 eliminates the labor 

intensive sample preparation steps 

(extraction and derivatization) that are 

required in the current methods that are 

approved for haloacetic acid 

determinations. It also reduces the use 

of solvents and potentially hazardous 

chemicals. The development work for 

this method is described in the method 

research summary (Zaffiro and 

Zimmerman 2009). 

The sum of five haloacetic acids 

(monochloroacetic acid, dichloroacetic 

acid, trichloroacetic acid, 

monobromoacetic acid, and 

dibromoacetic acid) is regulated as 

HAA5. The approved methods for 

HAA5 are listed at 40 CFR 

141.131(b)(1). The performance 

characteristics of EPA Method 557 for 

each of the five haloacetic acids were 

compared to the characteristics of 

approved EPA Methods 552.2 (USEPA 

1995) and 552.3, Revision 1.0 (USEPA 

2003) for the same compounds. EPA has 

determined that EPA Method 557 is 

equally effective for measuring HAA5 

relative to approved EPA Methods 552.2 

and 552.3. The basis for this 

determination is discussed in Munch 

2009b. Therefore, EPA is approving EPA 

Method 557 for determining HAA5 in 

drinking water. 

The performance characteristics of 

EPA Method 557 were also compared to 

the bromate-measurement 

characteristics of approved EPA 

Methods 300.1 (USEPA 2000), 317.0 

Revision 2.0 (USEPA 2001), and 326.0 

(USEPA 2002). EPA has determined that 

EPA Method 557 is equally effective for 

measuring bromate concentrations as 

these approved methods. EPA Method 

557 can also meet the MRL 

requirements necessary for methods that 

are used to support the reduced bromate 

monitoring specified at 40 CFR 

141.132(b)(3)(ii)(B). The basis for these 

determinations is discussed in Munch 

2009b. EPA is therefore approving EPA 

Method 557 for the routine 

determination of bromate in drinking 

water and also allowing its use for 

reduced bromate monitoring. 

A copy of EPA Method 557 can be 

accessed and downloaded directly online at http://epa.gov/safewater/ 

methods/ 

analyticalmethods_ogwdw.html. 

4. EPA Method 415.3, Revision 1.2, 

‘‘Determination of Total Organic Carbon 

and Specific UV Absorbance at 254 

nanometers (nm) in Source Water and 

Drinking Water’’ (USEPA 2009f) is a 

slightly modified version of the 

currently approved EPA Method 415.3, 

Revision 1.1 (USEPA 2005). Revision 

1.1 is listed as an approved method for 

determining total organic carbon (TOC), 

dissolved organic carbon (DOC), 

ultraviolet absorbance at 254 nm 

(UV254), and specific ultraviolet 

absorbance (SUVA) concentrations at 40 

CFR 141.131(d). Determination of UV254

can only be done using a double beam 

spectrophotometer if the instrument is 

zeroed according to the directions in the 

approved method. Since many water 

system laboratories use single beam 

spectrophotometers, the method was 

revised to allow for their use by 

modifying the zeroing procedure. This 

modification did not result in any 

change in the performance of the 

method. Therefore, EPA finds that 

Method 415.3, Revision 1.2 is equally 

effective as Revision 1.1. Revision 1.2 

also corrects some typographical errors 

that are present in Revision 1.1. The 

modifications are documented in 

Wimsatt 2009. EPA is approving EPA 

Method 415.3, Revision 1.2 for 

determining TOC, DOC, UV254, and 

SUVA in source water and drinking 

water. 

A copy of EPA Method 415.3, 

Revision 1.2 can be accessed and 

downloaded directly on-line at http:// 

www.epa.gov/nerlcwww/ordmeth.htm. 

B. Methods Developed by Voluntary 

Consensus Standard Bodies (VCSB) 

1. Standard Methods for the 

Examination of Water and Wastewater. 

Standard Methods 9223 B–97 and 9223 

B (20th and 21st Edition) can be used 

to detect Escherichia coli (E. coli). 

Approved methods for E. coli are listed 

at 40 CFR 141.21(f)(6). The Minimal 

Medium ONPG–MUG (MMO–MUG) 

Test is listed as an approved method for 

E. coli and the reference cited for the 

procedure is a journal article (Edberg et 

al. 1989). Standard Methods used the 

same research documented in the 

journal article to write Standard Method 

9223 B, which is published in the 20th 

and 21st Edition of Standard Methods 

for the Analysis of Water and 

Wastewater (APHA 1998, 2005). The 

same method is also available on-line as 

Standard Method 9223 B–97 (APHA 

1997). Since Standard Methods 9223 B 

(20th and 21st Edition) and 9223 B–97 

are the same procedure as that 

documented in the Edberg et al. article, 

they are equally effective as the 

approved Edberg method for 

determining E. coli (Best 2009). 

Therefore, EPA is approving the use of 

Standard Methods 9223 B (20th 

Edition), 9223 B (21st Edition) and 9223 

B–97 for determining E. coli as specified 

at 40 CFR 141.21(f)(6). The 20th and 

21st editions can be obtained from 

American Public Health Association 

(APHA), 800 I Street, NW., Washington, 

DC 20001–3710. Standard Method 9223 

B–97 is available at http:// 

www.standardmethods.org. 

2. ASTM International. EPA 

compared the most recent versions of 14 

ASTM International methods to the 

versions of those methods cited in 40 

CFR 141 and 143. Changes between the 

approved version and the most recent 

version of each method are summarized 


srobinson on DSKHWCL6B1PROD with RULES
57912 Federal Register / Vol. 74, No. 216 / Tuesday, November 10, 2009 / Rules and Regulations 

in Fair 2009. The revisions primarily 

involve editorial changes (i.e., updated 

references, reorganization, and 

corrections of errors). Data generated 

using the revised methods are 

comparable to data obtained using the 

previous versions because the 

chemistry, sample-handling protocols, 

and QC are unchanged. The new 

versions are equally effective relative to 

the version cited in the regulation (Fair 

2009). Therefore, EPA is approving the 

use of the 14 updated ASTM methods 

for the contaminants and regulations 

listed in the following table. 

The revised ASTM method for 

bromate and chlorite analyses (D 6581– 

08) is split into two techniques. Method 

A uses chemically suppressed ion 

chromatography and is the same as the 

approved Method D 6581–00, which is 

listed in the regulation at 40 CFR 

141.131(b)(1); ASTM D 6581–08 A is 

one of the 14 methods previously 

discussed. Method B uses 

electrolytically suppressed ion 

chromatography and represents a new 

method. EPA compared the bromate and 

chlorite performance data for Method B 

to the data in the approved Method D 

6581–00 and determined that Method B 

is equally effective as the currently 

approved method (Fair 2009). Therefore, 

EPA is approving ASTM D 6581–08 B 

for the determination of bromate and 

chlorite in routine drinking water 

compliance samples. 

The ASTM methods that are approved 

in this action are listed in the following 

table: 

ASTM method Contaminant Regulation 

D511–09 A (ASTM International 2009a) ................................................................................ Calcium .......................... 40 CFR 141.23(k)(1). 

Magnesium .................... 40 CFR 141.23(k)(1). 

D511–09 B (ASTM International 2009a) ................................................................................ Calcium .......................... 40 CFR 141.23(k)(1). 

Magnesium .................... 40 CFR 141.23(k)(1). 

D1688–07 A (ASTM International 2009b) .............................................................................. Copper ........................... 40 CFR 141.23(k)(1). 

D1688–07 C (ASTM International 2009b) .............................................................................. Copper ........................... 40 CFR 141.23(k)(1). 

D2972–08 B (ASTM International 2009c) .............................................................................. Arsenic ........................... 40 CFR 141.23(k)(1). 

D2972–08 C (ASTM International 2009c) .............................................................................. Arsenic ........................... 40 CFR 141.23(k)(1). 

D3559–08 D (ASTM International 2009d) .............................................................................. Lead ............................... 40 CFR 141.23(k)(1). 

D3645–08 B (ASTM International 2009e) .............................................................................. Beryllium ........................ 40 CFR 141.23(k)(1). 

D3697–07 (ASTM International 2009f) ................................................................................... Antimony ........................ 40 CFR 141.23(k)(1). 

D3859–08 A (ASTM International 2009g) .............................................................................. Selenium ........................ 40 CFR 141.23(k)(1). 

D3859–08 B (ASTM International 2009g) .............................................................................. Selenium ........................ 40 CFR 141.23(k)(1). 

D1253–08 (ASTM International 2009h) .................................................................................. Free Chlorine ................. 40 CFR 141.74(a)(2). 

40 CFR 141.131(c)(1) 

Total Chlorine ................ 40 CFR 141.74(a)(2). 

40 CFR 141.131(c)(1). 

Combined Chlorine ........ 40 CFR 141.131(c)(1). 

D516–07 (ASTM International 2009i) ..................................................................................... Sulfate ............................ 40 CFR 143.4(b). 

D6581–08 A (ASTM International 2009j) ............................................................................... Bromate ......................... 40 CFR 141.131(b)(1). 

Chlorite ........................... 40 CFR 141.131(b)(1). 

D6581–08 B (ASTM International 2009j) ............................................................................... Bromate ......................... 40 CFR 141.131(b)(1). 

Chlorite ........................... 40 CFR 141.131(b)(1). 

The 15 ASTM methods are available 

from ASTM International, 100 Barr 

Harbor Drive, West Conshohocken, PA 

19428–2959 or http://www.astm.org. 

C. Methods Developed by Vendors 

1. AMI Turbiwell Method (SWAN 

Analytische Instrumente AG 2009a) 

uses light emitting diode (LED) 

nephelometry to continuously measure 

turbidity in drinking water. The 

turbidimeter utilizes a non-contact light 

source design to avoid fouling of optical 

surfaces. The LED has an emission range 

of 415 nm to 780 nm with a peak 

spectral radiance of 562nm. The light 

beam from the emission LED impinges 

the water surface and is refracted. The 

detector measures the scattered light at 

an angle of 90°. A light barrier avoids 

measurement errors due to light 

reflections. The instrument is equipped 

with an internal data logger, or the data 

can be downloaded to a personal 

computer or central data system with 

appropriate computer software. 

The approved methods for turbidity 

are listed at 40 CFR 141.74(a)(1). The 

performance characteristics of the AMI 

Turbiwell turbidimeter were compared 

to the performance characteristics of 

approved EPA Method 180.1 (USEPA 

1993). The validation study report 

(SWAN Analytische Instrumente AG 

2009b) summarizes the results obtained 

from the turbidimeters placed in series 

at three different public water systems. 

One water system used ground water 

and the other two plants used surface 

water sources. Measurements included 

at least one filter backwash at each of 

the surface water plants. 

EPA has determined that the AMI 

Turbiwell Method is equally effective as 

approved EPA Method 180.1. The basis 

for this determination is discussed in 

the validation study report (SWAN 

Analytische Instrumente AG 2009b). 

Therefore, EPA is approving the AMI 

Turbiwell Method for determining 

turbidity in drinking water. A copy of 

the method can be downloaded from the 

National Environmental Methods Index 

(NEMI) at http://www.nemi.gov or 

obtained by contacting Markus 

Bernasconi, SWAN Analytische 

Instrumente AG, Studbachstrasse 13, 

CH–8340 Hinwil, Switzerland. 

2. ChloroSense (Palintest Ltd 2009a) 

is an electrochemical sensor method 

that measures free and total chlorine 

using disposable sensors. Free and 

combined available chlorine react with 

proprietary reagents on the sensor to 

create intermediate reaction products. 

These products are then detected 

electrochemically. The current that 

flows in each case is proportional to the 

amount of free available chlorine or 

total available chlorine in the sample. 

The sensors are pre-calibrated, and free 

and total chlorine concentrations are 

displayed upon completion of the 

analysis. 

Approved methods for determining 

free and total chlorine residuals in 

drinking water are listed in the tables at 

40 CFR 141.74(a)(2) and 40 CFR 

141.131(c)(1). The performance 

characteristics of ChloroSense were 

compared to the performance 

characteristics of approved Standard 

Methods 4500–Cl D (amperometric 

titration)(APHA 1998) and 4500–Cl G 

(DPD colorimetric)(APHA 1998). A 

variety of samples, including drinking 

water samples from both surface and 


srobinson on DSKHWCL6B1PROD with RULES
Federal Register / Vol. 74, No. 216 / Tuesday, November 10, 2009 / Rules and Regulations 57913 

ground water sources, were fortified 

with known chlorine concentrations 

and then analyzed by each method. The 

results are summarized in the validation 

study report (Palintest Ltd 2009b). 

EPA has determined that the 

ChloroSense Method is equally effective 

as approved Standard Methods 4500–Cl 

D and 4500–Cl G. The basis for this 

determination is discussed in the 

validation study report (Palintest Ltd 

2009b). Therefore, EPA is approving the 

ChloroSense Method for determining 

free and total chlorine residuals in 

drinking water. A copy of the method 

can be downloaded from NEMI at 

http://www.nemi.gov or obtained by 

contacting Palintest Ltd, 21 Kenton 

Lands Road, P.O. Box 18395, Erlanger, 

KY 41018. 

3. Modified ColitagTM (CPI 

International 2009). ColitagTM (CPI 

International 2001) is a presence/ 

absence method approved for use under 

the Total Coliform Rule. It uses 

enzymatic cleavage of a chromogenic 

substance to detect total coliforms and 

enzymatic cleavage of a fluorogenic 

substance to detect E. coli in a 100 mL 

sample of drinking water. Detection of 

total coliforms and E. coli are performed 

simultaneously by this method. 

ColitagTM may also be used in a mostprobable-number format provided that 

the sum of all individual portions of the 

sample total 100 mL. Modified 

ColitagTM has a different formulation 

from the originally approved ColitagTM. 

The purpose of the formula change is to 

achieve greater selectivity for total 

coliforms and E. coli. Additionally, the 

Modified ColitagTM provides flexibility 

in the incubation period (16 to 48 

hours), while the approved ColitagTM

requires a 24 hour incubation time. 

Approved methods for total coliforms 

are listed at 40 CFR 141.21(f)(3) and 

approved methods for E. coli are listed 

at 40 CFR 141.21(f)(6). The performance 

characteristics of Modified ColitagTM

were compared to Standard Methods 

9221 B (LTB/BGLB) for total coliforms 

and 9222 G (LTB/EC–MUG) for E. coli 

(APHA 1998). The comparison study 

involved analyses of twenty replicate 

drinking water samples that were 

inoculated with very low densities of 

chlorine stressed total coliforms or E. 

coli obtained from ten geographically 

dispersed waste waters. Method 

specificity was evaluated using 100 

positive and 100 negative cultures as 

determined from analyses by the 

reference methods. 

EPA has determined that the Modified 

ColitagTM Method is equally effective as 

approved Standard Methods 9221 B for 

total coliforms and 9222 G for E. coli, 

which are already promulgated in the 

regulations at 40 CFR 141.21(f)(3) and 

40 CFR 141.21(f)(6), respectively. The 

basis for this determination is discussed 

in the study report (USEPA 2009g). 

Therefore, EPA is approving the 

Modified ColitagTM Method for 

determining total coliforms and E. coli 

in drinking water. A copy of the method 

can be downloaded from NEMI at 

http://www.nemi.gov or obtained by 

contacting CPI International, 580 

Skylane Boulevard, Santa Rosa, CA 

95403. 

IV. Statutory and Executive Order 

Reviews 

As noted in Section II, under the 

terms of SDWA Section 1401(1), this 

streamlined method approval action is 

not a rule. Accordingly, the 

Congressional Review Act, 5 U.S.C. 801 

et seq., as added by the Small Business 

Regulatory Enforcement Fairness Act of 

1996, does not apply because this action 

is not a rule for purposes of 5 U.S.C. 

804(3). Similarly, this action is not 

subject to the Regulatory Flexibility Act 

because it is not subject to notice and 

comment requirements under the 

Administrative Procedure Act or any 

other statute. In addition, because this 

approval action is not a rule but simply 

makes alternative (optional) testing 

methods available for monitoring under 

SDWA, EPA has concluded that other 

statutes and executive orders generally 

applicable to rulemaking do not apply 

to this approval action. 

V. References 

American Public Health Association (APHA). 

1997. Standard Method 9223–97. 

Enzyme Substrate Coliform Test. 

Approved by Standard Methods 

Committee 1997. Standard Methods 

Online. (Available at http:// 

www.standardmethods.org.) 

American Public Health Association (APHA). 

1998. 20th Edition of Standard Methods 

for the Examination of Water and 

Wastewater, American Public Health 

Association, 800 I Street, NW., 

Washington, DC 20001–3710. 

American Public Health Association (APHA). 

2005. 21st Edition of Standard Methods 

for the Examination of Water and 

Wastewater, American Public Health 

Association, 800 I Street, NW., 

Washington, DC 20001–3710. 

ASTM International. 2009a. ASTM D 511–09. 

Standard Test Methods for Calcium and 

Magnesium in Water. ASTM 

International, 100 Barr Harbor Drive, 

West Conshohocken, PA 19428–2959. 

(Available at http://www.astm.org.) 

ASTM International. 2009b. ASTM D 1688– 

07. Standard Test Methods for Copper in 

Water. ASTM International, 100 Barr 

Harbor Drive, West Conshohocken, PA 

19428–2959. (Available at http:// 

www.astm.org.) 

ASTM International. 2009c. ASTM D 2972– 

08. Standard Test Methods for Arsenic in 

Water. ASTM International, 100 Barr 

Harbor Drive, West Conshohocken, PA 

19428–2959. (Available at http:// 

www.astm.org.) 

ASTM International. 2009d. ASTM D 3559– 

08. Standard Test Methods for Lead in 

Water. ASTM International, 100 Barr 

Harbor Drive, West Conshohocken, PA 

19428–2959. (Available at http:// 

www.astm.org.) 

ASTM International. 2009e. ASTM D 3645– 

08. Standard Test Methods for Beryllium 

in Water. ASTM International, 100 Barr 

Harbor Drive, West Conshohocken, PA 

19428–2959. (Available at http:// 

www.astm.org.) 

ASTM International. 2009f. ASTM D 3697– 

07. Standard Test Methods for Antimony 

in Water. ASTM International, 100 Barr 

Harbor Drive, West Conshohocken, PA 

19428–2959. (Available at http:// 

www.astm.org.) 

ASTM International. 2009g. ASTM D 3859– 

08. Standard Test Methods for Selenium 

in Water. ASTM International, 100 Barr 

Harbor Drive, West Conshohocken, PA 

19428–2959. (Available at http:// 

www.astm.org.) 

ASTM International. 2009h. ASTM D 1253– 

08. Standard Test Method for Residual 

Chlorine in Water. ASTM International, 

100 Barr Harbor Drive, West 

Conshohocken, PA 19428–2959. 

(Available at http://www.astm.org.) 

ASTM International. 2009i. ASTM D 516–07. 

Standard Test Method for Sulfate Ion in 

Water. ASTM International, 100 Barr 

Harbor Drive, West Conshohocken, PA 

19428–2959. (Available at http:// 

www.astm.org.) 

ASTM International. 2009j. ASTM D 6581– 

08. Standard Test Methods for Bromate, 

Bromide, Chlorate, and Chlorite in 

Drinking Water by Suppressed Ion 

Chromatography. ASTM International, 

100 Barr Harbor Drive, West 

Conshohocken, PA 19428–2959. 

(Available at http://www.astm.org.) 

Best, J. 2009. Memo to the record describing 

basis for expedited approval of Standard 

Methods 9223 B (20th Edition), 9223 B 

(21st Edition) and 9223 B–97 for 

determining E. coli as specified at 40 

CFR 141.21(f)(6). August 31, 2009. 

CPI International. 2001. ColitagTM Test. 

ColitagTM Product as a Test for Detection 

and Identification of Coliforms and 

Escherichia coli Bacteria in Drinking 

Water and Source Water as Required in 

National Primary Drinking Water 

Regulations. August 2001. 580 Skylane 

Boulevard, Santa Rosa, CA 95403. 

CPI International. 2009. Modified ColitagTM

Method. Modified ColitagTM Test 

Method for the Simultaneous Detection 

of E. coli and other Total Coliforms in 

Water (ATP D05–0035). August 28, 2009. 

5580 Skylane Boulevard, Santa Rosa, CA 

95403. 

Edberg, S.C. et al. 1989. ‘‘National Field 

Evaluation of a Defined Substrate 

Method for the Simultaneous Detection 

of Total Coliforms and Escherichia coli 

from Drinking Water: Comparison with 


srobinson on DSKHWCL6B1PROD with RULES
57914 Federal Register / Vol. 74, No. 216 / Tuesday, November 10, 2009 / Rules and Regulations 

Presence-Absence Techniques.’’ Applied 

Environmental Microbiology, Vol. 55, 

pp. 1003–1008, April 1989. 

Environmental Engineering & Technology, 

Inc. (EE&T, Inc.). Memorandum No. 4 to 

AWWA. Final Analysis of Online 

Amperometric Data. EE&T Project No. 

5318, February 27, 2009. EE&T, Inc., 712 

Gum Rock Court, Newport News, VA 

23606. 

Fair, P. 2009. Memo to the record describing 

basis for approval of updated ASTM 

methods. September 30, 2009. 

Fair, P. and Wendelken, S. 2009. Memo to 

the record describing basis for expedited 

approval of EPA Method 334.0. 

September 30, 2009. 

Munch, D. 2009a. Memo to the record 

describing basis for expedited approval 

of EPA Method 302.0 for bromate. 

September 30, 2009. 

Munch, D. 2009b. Memo to the record 

describing basis for expedited approval 

of EPA Method 557 for HAA5 and 

bromate. October 6, 2009. 

Palintest Ltd. 2009a. ChloroSense Method, 

Rev. 1.0. Measurement of Free and Total 

Chlorine in Drinking Water by Palintest 

ChloroSense, August 10, 2009, Palintest 

Ltd, 21 Kenton Lands Road, PO Box 

18395, Erlanger, KY 41018. (Available at 

http://www.nemi.gov.) 

Palintest Ltd. 2009b. ATP Evaluation of 

Palintest ChloroSense for the 

Measurement of Free and Total Chlorine, 

August 10, 2009. Palintest Ltd, 21 

Kenton Lands Road, PO Box 18395, 

Erlanger, KY 41018. 

SWAN Analytische Instrumente AG. 2009a. 

AMI Turbiwell Method, Rev. 1.0. 

Continuous Measurement of Turbidity 

Using a SWAN AMI Turbiwell 

Turbidimeter, August 10, 2009, Markus 

Bernasconi, SWAN Analytische 

Instrumente AG, Studbachstrasse 13, 

CH–8340 Hinwil, Switzerland. 

(Available at http://www.nemi.gov.) 

SWAN Analytische Instrumente AG. 2009b. 

ATP Evaluation of the SWAN AMI 

Turbiwell Turbidimeter for Measurement 

of Turbidity, August 10, 2009. 

Studbachstrasse 13, CH–8340 Hinwil, 

Switzerland. 

USEPA. 1993. EPA Method 180.1, Revision 

2.0, ‘‘Determination of Turbidity by 

Nephelometry’’ in Methods for the 

Determination of Inorganic Substances in 

Environmental Samples, EPA/600/R–93/ 

100. (Available at http://www.nemi.gov.) 

USEPA. 1995. EPA Method 552.2, 

‘‘Determination of Haloacetic Acids and 

Dalapon in Drinking Water by LiquidLiquid Extraction, Derivatization and 

Gas Chromatography with Electron 

Capture Detection’’ in Methods for the 

Determination of Organic Compounds in 

Drinking Water, Supplement III, EPA/ 

600/R–95–131, August 1995. (Available 

at http://www.nemi.gov.) 

USEPA. 2000. EPA Method 300.1, 

‘‘Determination of Inorganic Anions in 

Drinking Water by Ion Chromatography’’ 

in Methods for the Determination of 

Organic and Inorganic Compounds in 

Drinking Water, Volume 1, EPA 815–R– 

00–014. (Available at http:// 

www.epa.gov/safewater/methods/ 

analyticalmethods_ogwdw.html.) 

USEPA. 2001. EPA Method 317.0, Revision 

2.0, Determination of Inorganic 

Oxyhalide Disinfection By-Products in 

Drinking Water Using Ion 

Chromatography with the Addition of a 

Postcolumn Reagent for Trace Bromate 

Analysis, EPA 815–B–01–001, July 2001. 

(Available at http://epa.gov/safewater/ 

methods/ 

analyticalmethods_ogwdw.html.) 

USEPA. 2002. EPA Method 326.0, 

Determination of Inorganic Oxyhalide 

Disinfection By-Products in Drinking 

Water Using Ion Chromatography 

Incorporating the Addition of a 

Suppressor Acidified Postcolumn 

Reagent for Trace Bromate Analysis, EPA 

815–R–03–007, June 2002. (Available at 

http://epa.gov/safewater/methods/ 

analyticalmethods_ogwdw.html.) 

USEPA. 2003. EPA Method 552.3, 

Determination of Haloacetic Acids and 

Dalapon in Drinking Water by LiquidLiquid Microextraction, Derivatization, 

and Gas Chromatography with Electron 

Capture Detection, EPA 815–B–03–002, 

July 2003. (Available at http:// 

www.epa.gov/safewater/methods/ 

analyticalmethods_ogwdw.html). 

USEPA. 2005. EPA Method 415.0, Revision 

1.1. Determination of Total Organic 

Carbon and Specific UV Absorbance at 

254 nm in Source Water and Drinking 

Water. EPA/600/R–05/055, February 

2005. (Available at http://www.epa.gov/ 

nerlcwww/ordmeth.htm.) 

USEPA. 2007. Expedited Approval of Test 

Procedures for the Analysis of 

Contaminants Under the Safe Drinking 

Water Act; Analysis and Sampling 

Procedures. 72 FR 17902. April 10, 2007. 

USEPA. 2008. Expedited Approval of 

Alternative Test Procedures for the 

Analysis of Contaminants Under the Safe 

Drinking Water Act; Analysis and 

Sampling Procedures. 73 FR 31616. June 

3, 2008. 

USEPA. 2009a. Expedited Approval of 

Alternative Test Procedures for the 

Analysis of Contaminants Under the Safe 

Drinking Water Act; Analysis and 

Sampling Procedures. 74 FR 38348. 

August 3, 2009. 

USEPA. 2009b. EPA Method 334.0. 

Determination of Residual Chlorine in 

Drinking Water Using an On-line 

Chlorine Analyzer, EPA 815–B–09–013. 

September 2009. (Available at http:// 

epa.gov/safewater/methods/ 

analyticalmethods_ogwdw.html.) 

USEPA. 2009c. Response to Comments 

Document for Review of EPA Method 

334.0. September 30, 2009. 

USEPA. 2009d. EPA Method 302.0. 

Determination of Bromate in Drinking 

Waters using Two-Dimensional Ion 

Chromatography with Suppressed 

Conductivity Detection, EPA 815–B–09– 

014. September 2009. (Available at 

http://epa.gov/safewater/methods/ 

analyticalmethods_ogwdw.html.) 

USEPA. 2009e. EPA Method 557. 

Determination of Haloacetic Acids, 

Bromate, and Dalapon in Drinking Water 

by Ion Chromatography Electrospray 

Ionization Tandem Mass Spectrometry 

(IC–ESI–MS/MS), EPA 815–B–09–012, 

August 2009. (Available at http:// 

epa.gov/safewater/methods/ 

analyticalmethods_ogwdw.html.) 

USEPA. 2009f. EPA Method 415.0, Revision 

1.2. Determination of Total Organic 

Carbon and Specific UV Absorbance at 

254 nm in Source Water and Drinking 

Water. EPA/600/R–09/122, September 

2009. (Available at http://www.epa.gov/ 

nerlcwww/ordmeth.htm.) 

USEPA. 2009g. ATP Study Report of 

Modified ColitagTM, ATP Case No. D05– 

0035, September 21, 2009. 

Wimsatt, J. 2009. Memo to the record 

describing changes to EPA Method 

415.3, Revision 1.1 that are incorporated 

into Revision 1.2. September 30, 2009. 

Zaffiro, A.D. and Zimmerman, M. 2009. EPA 

Method 557 Research Summary, Shaw 

Environmental Inc., Cincinnati OH. 

March 2009. 

List of Subjects in 40 CFR Part 141 

Chemicals, Environmental protection, 

Indians—lands, Intergovernmental 

relations, Radiation protection, 

Reporting and recordkeeping 

requirements, Water supply. 

Dated: October 29, 2009. 

Peter S. Silva, 

Assistant Administrator, Office of Water. 

■ For the reasons stated in the preamble, 

40 CFR part 141 is amended as follows: 

PART 141—NATIONAL PRIMARY 

DRINKING WATER REGULATIONS 

■ 1. The authority citation for part 141 

continues to read as follows: 

Authority: 42 U.S.C. 300f, 300g–l, 300j–4, 

and 300j–9. 

■ 2. Appendix A to subpart C of part 

141 is amended as follows: 

■ a. By revising the entry in the table 

entitled ‘‘Alternative testing methods for 

contaminants listed at 40 CFR 

141.21(f)(3).’’ 

■ b. By adding the table entitled 

‘‘Alternative testing methods for 

contaminants listed at 40 CFR 

141.21(f)(6)’’ after the table entitled 

‘‘Alternative testing methods for 

contaminants listed at 40 CFR 

141.21(f)(3).’’ 

■ c. By revising the entries for 

‘‘Antimony,’’ ‘‘Arsenic,’’ ‘‘Beryllium,’’ 

‘‘Calcium,’’ ‘‘Copper,’’ ‘‘Lead,’’ 

‘‘Magnesium,’’ and ‘‘Selenium’’ in the 

table entitled ‘‘Alternative testing 

methods for contaminants listed at 40 

CFR 141.23(k)(1).’’ 

■ d. By revising the entry for 

‘‘Turbidity’’ in the table entitled 

‘‘Alternative testing methods for 

contaminants listed at 40 CFR 

141.74(a)(1).’’ 

■ e. By revising the entries for ‘‘Free 

Chlorine’’ and ‘‘Total Chlorine’’ in the 


srobinson on DSKHWCL6B1PROD with RULES
Federal Register / Vol. 74, No. 216 / Tuesday, November 10, 2009 / Rules and Regulations 57915 

table entitled ‘‘Alternative testing 

methods for disinfectant residuals listed 

at 40 CFR 141.74(a)(2).’’ 

■ f. By revising the entry for ‘‘HAA5’’ 

and adding the entries for ‘‘Bromate’’ 

and ‘‘Chlorite’’ after the entry for 

‘‘HAA5’’ in the table entitled 

‘‘Alternative testing methods for 

contaminants listed at 40 CFR 

141.131(b)(1).’’ 

■ g. By revising the entries for ‘‘Free 

Chlorine,’’ ‘‘Combined Chlorine’’ and 

‘‘Total Chlorine’’ in the table entitled 

‘‘Alternative testing methods for 

disinfectant residuals listed at 40 CFR 

141.131(c)(1).’’ 

■ h. By revising all the entries in the 

table entitled ‘‘Alternative testing 

methods for parameters listed at 40 CFR 

141.131(d).’’ 

■ i. By adding the table entitled 

‘‘Alternative testing methods with MRL 

≤ 0.0010 mg/L for monitoring listed at 

40 CFR 141.132(b)(3)(ii)(B)’’ after the 

table entitled ‘‘Alternative testing 

methods for parameters listed at 40 CFR 

141.131(d).’’ 

■ j. By revising the entry for ‘‘Sulfate’’ 

in the table entitled ‘‘Alternative testing 

methods for contaminants listed at 40 

CFR 143.4(b)’’ and, 

■ k. By adding footnotes 13 through 19 

to the table. 

Appendix A to Subpart C of Part 141— 

Alternative Testing Methods Approved 

for Analyses Under the Safe Drinking 

Water Act 

* * * * * 

ALTERNATIVE TESTING METHODS FOR CONTAMINANTS LISTED AT 40 CFR 141.21(f)(3) 

Organism Methodology SM 21st edition 1 Other 

Total Coliforms ............ Total Coliform Fermentation Technique ............................................ 9221 A, B 

Total Coliform Membrane Filter Technique ....................................... 9222 A, B, C 

Presence-Absence (P–A) Coliform Test ............................................ 9221 D 

ONPG–MUG Test .............................................................................. 9223 

Colitag TM ........................................................................................... Modified Colitag TM 13

ALTERNATIVE TESTING METHODS FOR CONTAMINANTS LISTED AT 40 CFR 141.21(F)(6) 

Organism Methodology SM 20th 

edition 6

SM 21st 

edition 1 SM online 3 Other 

E. coli .......................... ONPG–MUG Test ........................... 9223 B 9223 B 9223 B–97 

Modified Colitag TM 13

ALTERNATIVE TESTING METHODS FOR CONTAMINANTS LISTED AT 40 CFR 141.23(k)(1) 

Contaminant Methodology EPA method SM 21st 

edition 1 SM online 3 ASTM 4 Other 

* * * * * * * 

Antimony ............ Hydride-Atomic Absorption ........ D 3697–07 

Atomic Absorption; Furnace ...... 3113 B 

Axially viewed inductively coupled plasma-atomic emission 

spectrometry (AVICP–AES).

200.5, Revision 

4.2.2

Arsenic ............... Atomic Absorption; Furnace ...... 3113 B D 2972–08 C 

Hydride Atomic Absorption ........ 3114 B D 2972–08 B 

Axially viewed inductively coupled plasma-atomic emission 

spectrometry (AVICP–AES).

200.5, Revision 

4.2. 

* * * * * * * 

Beryllium ............. Inductively Coupled Plasma ...... 3120 B 

Atomic Absorption; Furnace ...... 3113 B D 3645–08 B 

Axially viewed inductively coupled plasma-atomic emission 

spectrometry (AVICP–AES).

200.5, Revision 

4.2. 

* * * * * * * 

Calcium .............. EDTA titrimetric ......................... 3500–Ca B D 511–09 A 

Atomic Absorption; Direct Aspiration.3111 B D 511–09 B 

Inductively Coupled Plasma ...... 3120 B 

Axially viewed inductively coupled plasma-atomic emission 

spectrometry (AVICP–AES).

200.5, Revision 

4.2. 

* * * * * * * 

Copper ................ Atomic Absorption; Furnace ...... 3113 B D 1688–07 C 

Atomic Absorption; Direct Aspiration.3111 B D 1688–07 A 

Inductively Coupled Plasma ...... 3120 B 


srobinson on DSKHWCL6B1PROD with RULES
57916 Federal Register / Vol. 74, No. 216 / Tuesday, November 10, 2009 / Rules and Regulations 

ALTERNATIVE TESTING METHODS FOR CONTAMINANTS LISTED AT 40 CFR 141.23(k)(1)—Continued 

Contaminant Methodology EPA method SM 21st 

edition 1 SM online 3 ASTM 4 Other 

Axially viewed inductively coupled plasma-atomic emission 

spectrometry (AVICP–AES).

200.5, Revision 

4.2. 

* * * * * * * 

Lead ................... Atomic Absorption; Furnace ...... 3113 B D 3559–08 D 

Axially viewed inductively coupled plasma-atomic emission 

spectrometry (AVICP–AES).

200.5, Revision 

4.2. 

Magnesium ......... Atomic Absorption ..................... 3111 B D 511–09 B 

Inductively Coupled Plasma ...... 3120 B 

Complexation Titrimetric Methods.3500–Mg B D 511–09 A 

Axially viewed inductively coupled plasma-atomic emission 

spectrometry (AVICP–AES).

200.5, Revision 

4.2. 

* * * * * * * 

Selenium ............ Hydride-Atomic Absorption ........ 3114 B D 3859–08 A 

Atomic Absorption; Furnace ...... 3113 B D 3859–08 B 

Axially viewed inductively coupled plasma-atomic emission 

spectrometry (AVICP–AES).

200.5, Revision 

4.2. 

******

* * * * * 

ALTERNATIVE TESTING METHODS FOR CONTAMINANTS LISTED AT 40 CFR 141.74(a)(1) 

Organism Methodology SM 21st 

edition 1 Other 

******

Turbidity ...................... Nephelometric Method .................................................................................. 2130 B 

Laser Nephelometry (on-line) ........................................................................ Mitchell M5271 10

LED Nephelometry (on-line) .......................................................................... Mitchell M5331 11

LED Nephelometry (on-line) .......................................................................... AMI Turbiwell 15

LED Nephelometry (portable) ........................................................................ Orion AQ4500 12

ALTERNATIVE TESTING METHODS FOR DISINFECTANT RESIDUALS LISTED AT 40 CFR 141.74(a)(2) 

Residual Methodology SM 21st 

edition 1 ASTM 4 Other 

Free Chlorine .............. Amperometric Titration ................................................... 4500–Cl D D 1253–08 

DPD Ferrous Titrimetric .................................................. 4500–Cl F 

DPD Colorimetric ............................................................ 4500–Cl G 

Syringaldazine (FACTS) ................................................. 4500–Cl H 

On-line Chlorine Analyzer .............................................. EPA 334.0 16

Amperometric Sensor ..................................................... ChloroSense 17

Total Chlorine .............. Amperometric Titration ................................................... 4500–Cl D D 1253–08 

Amperometric Titration (Low level measurement) ......... 4500–Cl E 

DPD Ferrous Titrimetric .................................................. 4500–Cl F 

DPD Colorimetric ............................................................ 4500–Cl G 

Iodometric Electrode ....................................................... 4500–Cl I 

On-line Chlorine Analyzer .............................................. EPA 334.0 16

Amperometric Sensor ..................................................... ChloroSense 17

******


srobinson on DSKHWCL6B1PROD with RULES
Federal Register / Vol. 74, No. 216 / Tuesday, November 10, 2009 / Rules and Regulations 57917 

ALTERNATIVE TESTING METHODS FOR CONTAMINANTS LISTED AT 40 CFR 141.131(b)(1) 

Contaminant Methodology EPA method ASTM 4 SM 21st edition 1

******

HAA5 ........................... LLE (diazomethane)/GC/ECD ........................................ 6251 B 

******

Ion Chromatography Electrospray Ionization Tandem 

Mass Spectrometry (IC–ESI–MS/MS).

557 14

Bromate ....................... Two-Dimensional Ion Chromatography (IC) ................... 302.0 18

Ion Chromatography Electrospray Ionization Tandem 

Mass Spectrometry (IC–ESI–MS/MS).

557 14

Chemically Suppressed Ion Chromatography ................ D 6581–08 A 

Electrolytically Suppressed Ion Chromatography ........... D 6581–08 B 

Chlorite ........................ Chemically Suppressed Ion Chromatography ................ D 6581–08 A 

Electrolytically Suppressed Ion Chromatography ........... D 6581–08 B 

ALTERNATIVE TESTING METHODS FOR DISINFECTANT RESIDUALS LISTED AT 40 CFR 141.131(c)(1) 

Residual Methodology SM 21st edition 1 ASTM 4 Other 

Free Chlorine .............. Amperometric Titration ................................................... 4500–Cl D D 1253–08 

DPD Ferrous Titrimetric ................................................. 4500–Cl F 

DPD Colorimetric ............................................................ 4500–Cl G 

Syringaldazine (FACTS) ................................................. 4500–Cl H 

Amperometric Sensor ..................................................... ChloroSense 17

On-line Chlorine Analyzer .............................................. EPA 334.0 16

Combined Chlorine ..... Amperometric Titration ................................................... 4500–Cl D D 1253–08 

DPD Ferrous Titrimetric ................................................. 4500–Cl F 

DPD Colorimetric ............................................................ 4500–Cl G 

Total Chlorine .............. Amperometric Titration ................................................... 4500–Cl D D 1253–08 

Low level Amperometric Titration ................................... 4500–Cl E 

DPD Ferrous Titrimetric ................................................. 4500–Cl F 

DPD Colorimetric ............................................................ 4500–Cl G 

Iodometric Electrode ...................................................... 4500–Cl I 

Amperometric Sensor ..................................................... ChloroSense 17

On-line Chlorine Analyzer .............................................. EPA 334.0 16

* * * * * 

ALTERNATIVE TESTING METHODS FOR PARAMETERS LISTED AT 40 CFR 141.131(d) 

Parameter Methodology SM 21st 

edition 1 EPA 

Total Organic Carbon (TOC) ................................ High Temperature Combustion ............................ 5310 B 415.3, Rev 1.2 19

Persulfate-Ultraviolet or Heated Persulfate Oxidation.5310 C 415.3, Rev 1.2 

Wet Oxidation ...................................................... 5310 D 415.3, Rev 1.2 

Specific Ultraviolet Absorbance (SUVA) .............. Calculation using DOC and UV254 data ............... 415.3, Rev 1.2 

Dissolved Organic Carbon (DOC) ................ High Temperature Combustion ............................ 5310 B 415.3, Rev 1.2 

Persulfate-Ultraviolet or Heated Persulfate Oxidation.5310 C 415.3, Rev 1.2 

Wet Oxidation ...................................................... 5310 D 415.3, Rev 1.2 

Ultraviolet absorption at 254 nm (UV254) ...... Spectrophotometry ............................................... 5910 B 415.3, Rev 1.2 

ALTERNATIVE TESTING METHODS WITH MRL > 0.0010 MG/L FOR MONITORING LISTED AT 40 CFR 141.132(b)(3)(ii)(B) 

Contaminant Methodology EPA method 

Bromate ............. Two-Dimensional Ion Chromatography (IC) ..................................................................................................... 302.0 18

Ion Chromatography Electrospray Ionization Tandem Mass Spectrometry (IC–ESI–MS/MS) ........................ 557 14

* * * * * 


srobinson on DSKHWCL6B1PROD with RULES
57918 Federal Register / Vol. 74, No. 216 / Tuesday, November 10, 2009 / Rules and Regulations 

ALTERNATIVE TESTING METHODS FOR CONTAMINANTS LISTED AT 40 CFR 143.4(b) 

Contaminant Methodology EPA Method ASTM 4 SM 21st edition 1 SM Online 3

******

Sulfate .............. Ion Chromatography ......................................... 4110 B 

Gravimetric with ignition of residue ................... 4500–SO4

¥2 C 4500–SO4¥2 C–97 

Gravimetric with drying of residue .................... 4500–SO4

¥2 D 4500–SO4¥ D–97 

Turbidimetric method ........................................ D 516–07 4500–SO4

¥2 E 4500–SO4¥2 E–97 

Automated methylthymol blue method ............. 4500–SO4

¥2 F 4500–SO4¥2 F–97 

******

1Standard Methods for the Examination of 

Water and Wastewater, 21st edition (2005). 

Available from American Public Health 

Association, 800 I Street, NW, Washington, 

DC 20001–3710. 2EPA Method 200.5, Revision 4.2. 

‘‘Determination of Trace Elements in 

Drinking Water by Axially Viewed 

Inductively Coupled Plasma-Atomic 

Emission Spectrometry.’’ 2003. EPA/600/R– 

06/115. (Available at http://www.epa.gov/ 

nerlcwww/ordmeth.htm.) 3Standard Methods Online are available at 

http://www.standardmethods.org. The year 

in which each method was approved by the 

Standard Methods Committee is designated 

by the last two digits in the method number. 

The methods listed are the only online 

versions that may be used. 4Available from ASTM International, 100 

Barr Harbor Drive, West Conshohocken, PA 

19428–2959 or http://astm.org. The methods 

listed are the only alternative versions that 

may be used. 

* * * * * 6Standard Methods for the Examination of 

Water and Wastewater, 20th edition (1998). 

Available from American Public Health 

Association, 800 I Street, NW., Washington, 

DC 20001–3710. 

* * * * * 10Mitchell Method M5271, Revision 1.1. 

‘‘Determination of Turbidity by Laser 

Nephelometry,’’ March 5, 2009. Available at 

http://www.nemi.gov or from Leck Mitchell, 

Ph.D., PE, 656 Independence Valley Dr., 

Grand Junction, CO 81507. 11Mitchell Method M5331, Revision 1.1. 

‘‘Determination of Turbidity by LED 

Nephelometry,’’ March 5, 2009. Available at 

http://www.nemi.gov or from Leck Mitchell, 

Ph.D., PE, 656 Independence Valley Dr., 

Grand Junction, CO 81507. 12Orion Method AQ4500, Revision 1.0. 

‘‘Determination of Turbidity by LED 

Nephelometry,’’ May 8, 2009. Available at 

http://www.nemi.gov or from Thermo 

Scientific, 166 Cummings Center, Beverly, 

MA 01915, http://www.thermo.com. 13Modified ColitagTM Method, ‘‘Modified 

ColitagTM Test Method for the Simultaneous 

Detection of E. coli and other Total Coliforms 

in Water (ATP D05–0035),’’ August 28, 2009. 

Available at http://www.nemi.gov or from 

CPI, International, 580 Skylane Boulevard, 

Santa Rosa, CA 95403. 14EPA Method 557. ‘‘Determination of 

Haloacetic Acids, Bromate, and Dalapon in 

Drinking Water by Ion Chromatography 

Electrospray Ionization Tandem Mass 

Spectrometry (IC–ESI–MS/MS),’’ August 

2009. EPA 815–B–09–012. Available at 

http://epa.gov/safewater/methods/analytical

methods_ogwdw.html. 15AMI Turbiwell, ‘‘Continuous 

Measurement of Turbidity Using a SWAN 

AMI Turbiwell Turbidimeter,’’ August 2009. 

Available at http://www.nemi.gov or from 

Markus Bernasconi, SWAN Analytische 

Instrumente AG, Studbachstrasse 13, CH– 

8340 Hinwil, Switzerland. 16EPA Method 334.0. ‘‘Determination of 

Residual Chlorine in Drinking Water Using 

an On-line Chlorine Analyzer,’’ August 2009. 

EPA 815–B–09–013. Available at http://epa.

gov/safewater/methods/analyticalmethods_

ogwdw.html. 17ChloroSense. ‘‘Measurement of Free and 

Total Chlorine in Drinking Water by Palintest 

ChloroSense,’’ September 2009. Available at 

http://www.nemi.gov or from Palintest Ltd, 

21 Kenton Lands Road, PO Box 18395, 

Erlanger, KY 41018. 18EPA Method 302.0. ‘‘Determination of 

Bromate in Drinking Waters using TwoDimensional Ion Chromatography with 

Suppressed Conductivity Detection,’’ 

September 2009. EPA 815–B–09–014. 

Available at http://epa.gov/safewater/

methods/analyticalmethods_ogwdw.html. 19EPA 415.3, Revision 1.2. ‘‘Determination 

of Total Organic Carbon and Specific UV 

Absorbance at 254 nm in Source Water and 

Drinking Water,’’ August 2009. EPA/600/R– 

09/122. Available at http://www.epa.gov/

nerlcwww/ordmeth.htm. 

[FR Doc. E9–27044 Filed 11–9–09; 8:45 am] 

BILLING CODE 6560–50–P 

DEPARTMENT OF HEALTH AND 

HUMAN SERVICES 

National Institutes of Health 

42 CFR Part 52 

[Docket No. NIH–2007–0929] 

RIN 0925–AA42 

Grants for Research Projects 

AGENCY: National Institutes of Health, 

Department of Health and Human 

Services. 

ACTION: Final rule. 

SUMMARY: The National Institutes of 

Health is amending the current 

regulations governing grants for research 

projects by revising the definition of 

Principal Investigator to mean one or 

more individuals designated by the 

grantee in the grant application and 

approved by the Secretary, who is or are 

responsible for the scientific and 

technical direction of the project, rather 

than limiting the role of Principal 

Investigator to one single individual; 

and the conditions for multiple or 

concurrent awards pursuant to one or 

more applications. 

DATES: This final rule is effective 

December 10, 2009. 

FOR FURTHER INFORMATION CONTACT: Jerry 

Moore, NIH Regulations Officer, Office 

of Management Assessment, National 

Institutes of Health, 6011 Executive 

Boulevard, Room 601, MSC 7669, 

Rockville, MD 20852–7669, or 

telephone 301–496–4607. 

SUPPLEMENTARY INFORMATION: On 

September 30, 2003, the NIH Director 

announced a series of far reaching 

strategic initiatives known collectively 

as the NIH Roadmap for Medical 

Research (NIH Roadmap). The NIH 

Roadmap is an innovative approach 

designed to transform the Nation’s 

medical research capabilities and 

accelerate fundamental research 

discovery and translation of that 

knowledge into effective prevention 

strategies and new treatments. One of 

the NIH Roadmap initiatives encourages 

interdisciplinary research and team 

science and includes a recommendation 

to modify grant and research contract 

applications to allow for the proposing 

of more than one Principal Investigator 

when appropriate. This is congruent 

with the January 4, 2005, directive 

issued by the Office of Science and 

Technology Policy (OSTP) to all Federal 

research agency heads instructing the 

heads to accommodate the recognition 

of two or more Principal Investigators 

on research projects (grants and 

contracts). This OSTP policy does not 

prohibit the use of a single Principal 


srobinson on DSKHWCL6B1PROD with RULES