RTI Project No.: 0209408 .004 Protocol No. RTI

- 935 Final Report



### i SUMMARY

Eight m ale Fischer 344 rats were exposed to 14 

### C tertiary butyl alcohol in a nose

- only inhalation

system at a target concentration of 1750 ppm for 6 hours (actual concentration 1724 ± 85 ppm)

.

Following exposure, 4 rats were euthanized immediately and the radioactivity in the carcass was

determined by digestion and scintillation counting (Group 1 , Study A )

. The remaining f our rats were

transferred to all

- glass metabolism cages, and urine, fe ces, exhaled CO 2 

### and exhaled volatiles were

collected for seven days (Group 2 , Study B )

. After seven days, t he rats were euthanized and the

radioactivity remaining in the carcass was determined by digestion and scintillation counting.

Radioactivity was excreted primarily in urine (71 % of the recovered radioactivity). A lesser amount was

recovered as exhaled volatiles (25% of the recovered radioactivity). Approximately 0.6% was recovered

as 14 

### CO 2 

### , 1.7% in feces, and 0.6% in carcass at 7 days. Based on the recovered radioactivity, the

estimated dose in the rats in Group 1 was 532 ± 38 mg/kg, and in Group 2 was 510 ± 40 mg/kg.

Comparison of the exposure of rats to 1750 ppm [2

- 14 

### C]TBA/TBA euthanized immediately following

exposure (532 ± 38 mg/kg) with that of rats euthanized following 7 days of collection of excreta and

exhaled breath ( 510 ± 40 mg/kg ) yielded values that were very similar. This suggested that there were no

significant losses of radioactivity from the various sample types during proces sing of radioactivity.

Elimination of radioactivity was rapid, with 86% and 96% at of the radioactivity recovered within 24 h and

48 h , respectively, following the end of exposure. In urine collected at 12 h, three metabolites and 14 

### C

TBA were detected by HPLC with radioactivity detection. By 24 hours, 14 

### C TBA was detected in 2/4 urine

samples, and by 48 h was not detected. The three metabolites were tentatively identified as 2

- methyl

1,2

- propanediol, TBA glucuronide, and a glucuronide of α

- hydroxyisobut yric acid. Unchanged TBA

accounted for a major portion of the exhaled radioactivity.RTI Project No.: 0209408 .004 Protocol No. RTI

- 935 Final Report



### ii Quality Assurance StatementRTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### iv Table of Contents

Page

1.0 INTRODUCTION

................................ ................................ ................................ .............................. 1

2.0 OBJECTIVES AND PROTO COL

................................ ................................ ................................ .... 1

3.0 M ATERIALS AND METHODS

................................ ................................ ................................ ........ 1

3.1 Test Substance

................................ ................................ ................................ ................................ 1

3.1.1 Nonradiolabeled tertiary Butyl Alcohol

................................ ................................ ............. 2

3.1.2 [2

- 14 

### C]

- labeled tertiary Butyl Alcohol

................................ ................................ ................ 2

3.2 Reference Substances

................................ ................................ ................................ ..................... 2

3.3 Test System

................................ ................................ ................................ ................................ ..... 3

3.4 Test Chemical Preparation and Analysis

................................ ................................ ......................... 4

3.5 Inhalation Exposure

................................ ................................ ................................ ......................... 5

3.5.1 Exposure Analysis

................................ ................................ ................................ ................................ 5

3.6 Collection of Samples

................................ ................................ ................................ ...................... 5

3.6.1 Excreta

................................ ................................ ................................ ............................. 5

3.6.2 Carcass

................................ ................................ ................................ ............................ 6

3.7 Analysis of Samples for Total Radioactivity

................................ ................................ ..................... 6

3.7.1 Excreta

................................ ................................ ................................ ............................. 6

3.7.2 Carcass

................................ ................................ ................................ ............................ 7

3.7.3 Exhaled Breath Traps

................................ ................................ ................................ ............ 7

3.7.4 Exhaled Volatiles Traps

................................ ................................ ................................ ......... 7

3.8 HPLC Analysis of Urine Samples and Timed Fractions Collected from HPLC for

Radioactivity

................................ ................................ ................................ ................................ ..... 8

3.9 Determination of TBA in Exhaled Br eath Trap Extracts.

................................ ................................ .. 8

3.10 Data Collection and Reporting

................................ ................................ ................................ ......... 9

4.0 RESULTS

................................ ................................ ................................ ................................ ...... 10

4.1 Analysis of Radiolabeled Test Chemical Identity and Purity

................................ .......................... 10

4.2 Test Chemical Formulation

................................ ................................ ................................ ............ 10

4.3 Inhal ation exposure

................................ ................................ ................................ ........................ 10

4.4 Radioactivity Retained Following Exposure

................................ ................................ ................... 11

4.5 Excretion of Radioactivity

................................ ................................ ................................ ............... 11

4.6 TBA in Exhaled VOC Traps

................................ ................................ ................................ ........... 13

4.7 Excretion of TBA and Metabolites in Urine

................................ ................................ .................... 13RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### Table of Contents

(continued)

Page

v 5. 0 CONCLUSION

................................ ................................ ................................ ............................... 13

6.0 REFERENCES

................................ ................................ ................................ ............................... 14

7.0 RECORDS AND REPORTS

................................ ................................ ................................ .......... 15

8.0 STORAGE OF RECORDS A ND BIOLOGICAL SAMPLE S

................................ ......................... 15

9.0 REGULATORY COMPLIANC E

................................ ................................ ................................ ..... 16

10.0 STUDY PARTICIPANTS

................................ ................................ ................................ ............... 16RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### vi List of Tables

Page

Table 1. Male Fischer 344 Rats Used on the Study

................................ ................................ ................. 17

Table 2. Exposure Cond itions and Concentration of [2

- 14 

### C]TBA/TBA.

................................ .................... 18

Table 3. Total Recovery of Radioactivity following Exposure to 1750 ppm [2

- 14 

### C]TBA/TBA

................... 19

Table 4. Radioactivity in Group 1 Study A Rats Following Exposure to 1750 ppm [2

-

14 

### C]TBA/TBA, Expressed as a Percentage of the Total Radioactivity Recovered

.................... 20

Table 5. Radioact ivity in Excreta and Carcass following Exposure to 1750 ppm [2

- 14 

### C]TBA/TBA

(Group 2, Study B) Expressed as a Percentage of the Total Radioactivity Recovered

............ 21

Table 6. Radioactivity in Urin e following Exposure to 1750 ppm [2

- 14 

### C]TBA/TBA (Group 2, Study

B) Expressed as a Percentage of the Total Radioactivity Recovered

................................ ....... 22

Table 7. Radioactivity in Feces following Exposure to 175 0 ppm [2

- 14 

### C]TBA/TBA (Group 2,

Study B) Expressed as a Percentage of the Total Radioactivity Recovered

............................. 23

Table 8. Radioactivity in CO2 Trap 1 following exposure to 1750 ppm 14 

### C TBA (Group 2, Study

B) Expressed as a Percentage of the Total Radioactivity Recovered

................................ ....... 24

Table 9. Radioactivity in CO2 Trap 2 following exposure to 1750 ppm [2

- 14 

### C]TBA/TBA (Group 2,

Study B) Expressed as a Percentage of the Total Radioactivity Recovered

............................. 25

Table 10. Radioactivity in Exhaled VOC Trap 1 following exposure to 1750 ppm 14 

### C TBA

(Group 2, Study B) Expressed as a Percentage of t he Total Radioactivity Recovered

............ 26

Table 11

. Radioactivity in VOC Trap 1 Extract 2 following Exposure to 1750 ppm [2

-

14 

### C]TBA/TBA (Group 2, Study B) Expressed as a Percentage of the Total Ra dioactivity

Recovered

................................ ................................ ................................ ................................ .. 27

Table 12. Radioactivity in Exhaled VOC Trap 2 following Exposure to 1750 ppm [2

-

14 

### C]TBA/TBA (Group 2, Study B) Expressed as a Percentage of the Total Radioactivity

R ecovered

................................ ................................ ................................ ................................ .. 28

Table 13. Cumulative Recovery of Radioactivity in Excreta and Carcass following Exposure to

1750 ppm [2

- 14 

### C]TBA/TBA (Group 2, Study B) Expressed as a Percentage of the Total

Radi oactivity Recovered

................................ ................................ ................................ ............ 29

Table 14. TBA Measured by GC in DMF Extracts of VOC Trap 1 1 

### following Exposure to 1750

ppm [2

- 14 

### C]TBA/TBA

................................ ................................ ................................ .................. 30

Table 15. Percentage of Radioactivity as TBA in the DMF Extracts of VOC Trap 1 1 

### following

Exposure to 1750 ppm [2

- 14 

### C]TBA/TBA

................................ ................................ .................... 31

Table 16. Analysis of Radioactivity in Urine followin g Exposure to 1750 ppm [2

- 14 

### C]TBA/TBA

............. 32

Sample Naming and Abbreviations

................................ ................................ ................................ ............... 2

In the tables of raw data values in this appendix, th e table headings are derived from the

conventions of sample naming used in the Debra  system. These are explained

below. Example calculations and formulae are provided in Appendix G.

................................ ... 2

Table 1. Preparation of 14 

### C TBA Formulation (Feedstock) for Inhalation Exposure

................................ .... 4

Table 2. Recovery of Radioactivity in Samples from Group 1.

................................ ................................ .... 5

Table 3. Total Recovery of Radioactivity in Samples from Group 1.

................................ ........................... 6

Table 4. Recovery of Radioactivity in Urine from Group 2.

................................ ................................ .......... 7

Table 4 (contd). Recovery of Radioactivity in Urine from Group 2.

................................ ............................. 8RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### vii Table 5. Recovery of Radioactivity in Feces from Group 2.

................................ ................................ ........ 9

Table 5 (contd). Recovery of Radioactivity in Feces from Group 2.

................................ .......................... 10

Table 6. Recovery of Radioactivity in CO 2 

### Trap 1 from Group 2.

................................ .............................. 11

Table 6 (contd). Recovery of Radioactivity in CO 2 

### Trap 1 from Group 2.

................................ .................. 12

Table 7. Recovery of Radioactivity in CO 2 

### Trap 2 from Group 2.

................................ .............................. 13

Table 7 (contd). Recovery of Radioactivity in CO 2 

### Trap 2 from Group 2.

................................ .................. 14

Table 8. Recovery of Radioactivity in Exhaled VOC Trap 1 from Group 2.

................................ ............... 15

Table 8 (contd). Recovery of Radioactivity in Exhaled VOC Trap 1 from Group 2.

................................ ... 16

Table 9. Recovery of Radioactivity in VOC Trap 1 Extract 2 from Group 2.

................................ .............. 17

Table 10. Recovery of Radioactivity in Exhaled VOC Trap 2 from Group 2.

................................ ............. 18

Table 10 (contd). Rec overy of Radioactivity in Exhaled VOC Trap 2 from Group 2.

................................ 19

Table 11. Recovery of Radioactivity in Nose Only Tube Rinse from Group 2.

................................ .......... 20

Table 12. Recovery of Radioactivity in Transfer Bag Rinse from Group 2.

................................ ............... 20

Table 13. Recovery of Radioactivity in Cage Rinse from Group 2.

................................ ........................... 20

Table 14. Recovery of Radioactivity in Carcass Digest from Group 2.

................................ ...................... 21

Table 15. Total Recovery of Radioactivity in Samples from Group 2.

................................ ....................... 22

Table 15 (contd). Total Recovery of Radioactivity in Samples from Group 2.

................................ ........... 23

Table 15 (contd). Total Recovery of Radioactivity in Samples from Gro up 2.

................................ ........... 24

Table 15 (contd). Total Recovery of Radioactivity in Samples from Group 2.

................................ ........... 25

Table 15 (contd). Total Recovery of Rad ioactivity in Samples from Group 2.

................................ ........... 26

Table 16. Concentration (mg equiv./g) of Radioactivity in Exhaled VOC Trap Extracts from Group

2.

................................ ................................ ................................ ................................ ................ 27

Table 16 (contd). Concentration (mg equiv./g) of Radioactivity in Exhaled VOC Trap Extracts

from Group 2.

................................ ................................ ................................ ............................. 28

Table 16 (contd). Concentration (mg equiv./g) of Radioactivity in Ex haled VOC Trap Extracts

from Group 2.

................................ ................................ ................................ ............................. 29

Table 17. Concentration of TBA in Exhaled VOC Trap Extracts from Group 2.

................................ ........ 30

Table 17 (contd). Concentration of TBA in Exhaled VOC Trap Extracts from Group 2.

............................ 31

Table 17 (contd). Concentration of TBA in Exhaled VOC Trap Extracts from Group 2.

............................ 32

Table 17 (contd). Concentration of TBA in Exhaled VOC Trap Extracts from Group 2.

............................ 33

List of Figures

Page

Figu re 1. Cumulative Excretion of Radioactivity in Urine and Feces.

................................ ........................ 33

Figure 2. HPLC Analysis of Urinary Metabolites from Rat 2

- 06 Following Exposure to 1750 ppm

[2

- 14 

### C]TBA/TBA.

................................ ................................ ................................ ......................... 34

Figure 3. HPLC Separation of 2

- Methyl

- 1,2

- propanediol, α

- Hydroxyisobutyric Acid, and TBA.

............... 35RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### viii Figure 4. HPLC Analysis of Urinary Metabolite s from Rat 2

- 06 after Incubation with β

Glucuronidase or Acid Treatment

................................ ................................ .............................. 36

List of Appendices

Appendix A: Approved Study Protocol, Amendments, and Deviations

Appendix B: Test Chemical Analysis Report Tertiary Butyl Alcohol RTI Reference 12323

- 05

Appendix C: Test Chemical Analysis Report [2

- 14 

### C] Tertiary Butyl Alcohol RTI Reference 12323

- 11

Appendix D: Reference Standards Analysis Report 2

- Methylpropane

- 1,2

- diol and 2

- Hydroxyisobutyric

Acid

Appendix E: Prestudy Inhalatio n Report

Appendix F: Inhalation Report

Appendix G Equations Used in Calculations

Appendix H Raw Data TablesRTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### ix List of Tables in Appendix H

Table 1. Preparation of 14 

### C TBA Form ulation (Feedstock) for Inhalation Exposure

................................ H

- 4

Table 2. Recovery of Radioactivity in Samples from Group 1.

................................ ................................ H

- 5

Table 3. Total Recovery of Radioactivity in Samples from Group 1.

................................ ....................... H

- 6

Table 4. Recovery of Radioactivity in Urine from Group 2.

................................ ................................ ...... H

- 7

Table 4 ( contd). Recovery of Radioactivity in Urine from Group 2.

................................ ......................... H

- 8

Table 5. Recovery of Radioactivity in Feces from Group 2.

................................ ................................ .... H

- 9

Tab le 5 (contd). Recovery of Radioactivity in Feces from Group 2.

................................ ...................... H

- 10

Table 6. Recovery of Radioactivity in CO 2 

### Trap 1 from Group 2.

................................ .......................... H

- 11

Table 6 (contd). Recovery of Radioactivity in CO 2 

### Trap 1 from Group 2.

................................ .............. H

- 12

Table 7. Recovery of Radioactivity in CO 2 

### Trap 2 from Group 2.

................................ .......................... H

- 13

Table 7 (contd). Recovery of Radioactivity in CO 2 

### Trap 2 from Group 2.

................................ .............. H

- 14

Table 8. Recovery of Radioactivity in Exhaled VOC Trap 1 from Group 2.

................................ ........... H

- 15

Table 8 (contd). Recovery of Radioactivity in Exhaled VOC Trap 1 from Group 2.

............................... H

- 16

Table 9. Recovery of Radioactivity in VOC Trap 1 Ext ract 2 from Group 2.

................................ .......... H

- 17

Table 10. Recovery of Radioactivity in Exhaled VOC Trap 2 from Group 2.

................................ ......... H

- 18

Table 10 (contd). Recov ery of Radioactivity in Exhaled VOC Trap 2 from Group 2.

............................ H

- 19

Table 11. Recovery of Radioactivity in Nose Only Tube Rinse from Group 2.

................................ ...... H

- 20

Table 12. Recovery of Radioactivity in Transfer Bag Rinse from Group 2.

................................ ........... H

- 20

Table 13. Recovery of Radioactivity in Cage Rinse from Group 2.

................................ ....................... H

- 20

Table 14. Recovery of Radioactivity in Carcass Digest from Group 2.

................................ .................. H

- 21

Table 15. Total Recovery of Radioactivity in Samples from Group 2.

................................ ................... H

- 22

Table 15 (contd). Total Recovery of Radioactivity in Samples from Group 2.

................................ ....... H

- 23

Table 15 (contd). Total Recovery of Radioactivity in Samp les from Group 2.

................................ ....... H

- 24

Table 15 (contd). Total Recovery of Radioactivity in Samples from Group 2.

................................ ....... H

- 25

Table 15 (contd). Total Recovery of Radioactivity in Samples from Group 2.

................................ ....... H

- 26

Table 16. Concentration (mg equiv./g) of Radioactivity in Exhaled VOC Trap Extracts from Group

2.

................................ ................................ ................................ ................................ ............ H

- 27

Table 16 (contd). Concentration (mg equiv./g) of Radioactivity in Exhaled VOC Trap Extracts

from Group 2.

................................ ................................ ................................ ......................... H

- 28

Table 16 (contd). Concentration (mg equiv./g) of Radioactivity in Exhaled VOC Trap Extracts

from Group 2.

................................ ................................ ................................ ......................... H

- 29

Table 17. Concentration of TBA in Exhaled VOC Trap Extracts from Group 2.

................................ .... H

- 30

Table 17 (contd). Concentration of TBA in Exhaled VOC Trap Extracts from Group 2.

........................ H

- 31

Table 17 (contd). Concentration of TBA in Exhaled VOC Trap Extracts from Group 2.

........................ H

- 32

Table 17 (contd). Concentration of TBA in Exhaled VOC Trap Extracts from Group 2.

........................ H

- 33RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 1 1.0 INTRODUCTION

The purpose of this pilot study was to develop and validate procedures to be used to evaluate the

adsorption, distribution, metabolism, excretion and pharmacoki n etics of tertiary butyl alcohol (TBA) in rats.

TBA is used as an oxygenate additive to gasoline.

Toxicity studies have been conducted in rats and mice (Mahler 1997)

. A lpha 2u

- g lobulin

nephropathy has been reported in rats exposed to TBA by inhalation (Borghoff et al., 2001)

. TBA has

been demonstrated to bind to alpha 2u

- g lobulin in vivo (Williams and Borghoff 2001)

.

Limited s tudies have been conducted on the metabolism and disposition of TBA. Baker et al.

(1982) reported the metabolism of TBA to produce CO 2 

### and acetone in vivo in rats. The

pharmacokinetics of TBA were evaluated in mice (Faulkner and Hussain 1989) and in rats (Poet et al.,

1997)

.

2.0 OBJECTIVES AND PROTO COL

The objectives of this pilot study we re to:

Study A

1 ) conduct a nose

- only exposure of rats to 14 

### C TBA / TBA (n=4; 1 backup unexposed)

.

2 ) analy ze the amount of 14 

### C in whole body digest s from rats exposed to 14 

### C TBA/TBA.

Stud y B

3 ) conduct a nose

- only exposure of rat s to 14 

### C TBA / TBA (n=4; 2 backup unexposed)

.

4 ) collec t excreta from rats exposed to 14 

### C TBA / TBA.

5 ) analyze 14 

### C in whole body digest s , urine, feces, exhaled C O 2 

### , and exhale d volatiles from rats

exposed to 14 

### C TBA / TBA.

6 ) develop an HPLC method for analysis of urinary met abolites

.

7) analyze TBA in extracts from exhaled volatiles.

Copies of the approved protocol and protocol amendments for these studies are included in

Appendix A.

3.0 MATERIALS AND METHOD S

3.1 Test Substance

NAME : **tertiary** Butyl Alcohol (TBA; 2

- methyl

- 2

- propanol, CAS No. 75

- 65

- 0 

### )

MOLECULAR FORMULA : C 4 

### H 10 

### O

MOLECULAR WEIGHT : 74.12RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 2 STRUCTURE:

H 3 

### C C

C H 3 

### O H

H 3 

### C



### 3.1.1 Nonradiolabeled tertiary Butyl Alcohol

Source: Sigma

- Aldri ch, Milwaukee, WI

Product No: 36053

- 8

Lot No. : 01060AD

Purity: The chemical purity of **tertiary** Butyl Alcohol was characterized by Sigma

Aldrich at 99.6 %.

The unlabeled test material was characterized at RTI by gas chromatographic (GC) analysis for

purity and stability, and for identify by 1 

### H and 13 

### C Nuclear Magnetic Resonance (NMR)

spectroscopy and by GC

- mass spectroscopy. Details of the analysis are included in Appendix B.

3.1.2 [2

- 14 

### C]

- labeled tertiary Butyl Alcohol

Position of radiolabel: [2

- 14 

### C]

-

Source: Sigma Radiochemicals, prepared as a custom synthesis

Specific Activity: 7.3 mCi/mmol

Product No. : A8538

- 14C

Lot No. : 095K9406

Identity: The identity of [2

- 14 

### C]

- tertiary Butyl Alcohol was confirmed by

chromatographic comparison with unlabeled TBA using HPLC coupled

with radioactivity detection and refractive index detection

.

Purity: The radiochemical purity of [2

- 14 

### C]

- tertiary Butyl Alcohol was determined

chromatographically to be 98.2 %, using the HPLC system described in

Section 3. 8

.

The labele d test material was characterized at RTI by HPLC coupled with radioactivity detection

for purity and stability, and for identify by 1 

### H NMR spectroscopy and by HPLC coupled with

radioactivity detection and refractive index detection . Details of the analysi s are included in

Appendix C.

3.2 Reference Substances

2

- Hydroxyisobutyric acid (HBA) was obtained from Alfa Aesar. The purity specified by the

Vendor’s certificate of analysis was 98.9%. This material was assigned a chemical receipt number of

BOC

- B

- 0439. Identity of the material was verified by 1 

### H and 13 

### C NMR spectroscopy.

2

- Methylpropane

- 1,2

- diol was prepared at RTI using the method of Bernauer et al. (1998).RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 3 3. 3 Test System

**Source:** M ale Fischer 344 rats were purchased from Charles River ( Kingston , NY). Animal

weights at the time they were used in studies are shown in Table 1.

**Diet:** Animals were fed Certified Purina Rodent Chow #5002 and were furnished tap water ad libitum

.

The analysis of each feed batch for nutrient levels and possibl e contaminants was performed by the

supplier, examined by the Study Director, and maintained in the study records. The feed was stored at

approximately 60

− 70 ° F, and the period of use did not exceed six months from the milling date. The

source of the wat er was the City of Durham, NC. Approximately once a year, the City of Durham provides

analyses of the drinking water for potential contaminants. Documentation of these analyses were

inspected by the Study Director and maintained in the study records. In addition, samples of water were

collected during the study for analysis of tertiary butyl alcohol, tertiary amyl methyl ether, ethyl tertiary

butyl ether, diisopropyl ether, and methyl tertiary butyl ether. The samples were sent to Kiff Analytical

(Davi s, CA 95616) for analysis. Errors in sampling, shipping or receiving the first sample resulted in no

data being obtained for the prestudy sample. In two subsequent samples tertiary amyl methyl ether, ethyl

tertiary butyl ether, diisopropyl ether, and me thyl tertiary butyl ether were below the limits of quantitation.

Tertiary butyl alcohol was measured at 2.2 μ g/L (with 0.5 μ g/L as the reporting limit) in a sample collected

during the in

- life portion of the study. In a subsequent sample collected after the study, tertiary butyl

alcohol was below the reporting limit of 1.0 μ g/L. The California Office of Environmental Health Hazard

As sessment (OEHHA) (CALEPA 1999) developed an interim drinking water level of 12 μg/L based on the

incidence of kidney tumors reported in male rats

. Although a single measurement of tertiary butyl alcohol

was elevated, it is unlikely to have impacted the st udy, since the amount of TBA that could be consumed

,

assuming drinking water consumption of 0.028 L /day, would be equivalent to a total potential dose

arising from drinking water of 0.4 μ g TBA/rat over the course of the 7

- day study. ( Total consumption wa s

c alculated assuming a drinking water consumption rate of 0. 028 L/ day (USEPA, 1988)). This would be

insignificant compared to the total recovered dose, which ranged from 89

– 111 mg equiv. of TBA/rat.

**Identification:** Individual ear tags were used to uniquely identify animals used.

**Housing:** Rats were housed (maximum of three per cage) in polycarbonate cages with stainless

steel bar lids accommodating a water bottle until they were used in an experiment. Cage sizes are 19" x

10.5" x 8" high (143 sq. in. floor space). Contact bedding was Sani

- Chips hardwood chips (P. J. Murphy

Forest Products Co.; Montville, NJ).

Following dosing, the animals were housed in individual glass metabolism chambers ( for

collection of blood, urine, feces , exhaled CO 2 

### , and e xhaled volatiles ).

**Quarantine:** Rats were quarantined for a minimum of one week before use on a study. A

veterinarian or qualified designee examined the animals prior to their release from quarantine.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 4 **Randomization:** The ear tag numbers of animals were assigned in numerical order to sequential

values using a series of computer gen erated numbers as described in SOP DPK

- HUS

- 001 Assignment of

Animals into Groups, using the procedure for Assignment of Animals within a Single Group or Multiple

Groups without Regard to Weight Mean and Range. Four groups were prepared: Study A (n=4), Study B

(n=4), Study C (n=2), and Study D (n=1).

**Environmental:** Temperature and relative humidity in RTI animal rooms were continuously

monitored, controlled, and recorded usin g an automated system (Siebe/Barber

- Colman Network 8000

System with Revision 4.4.1 for Signal  software [Siebe Environmental Controls (SEC)/Barber

- Colman

Company; Loves Park, IL]). The target environmental ranges were 6 4

− 7 9 ° F ( 18 ° C

- 2 6 ° C) for

temperat ure and 3 0

− 70% relative humidity, with a 12

- h light cycle per day. The actual ranges observed

during quarantine were 71.07

− 72.8 ° F and 52.2

− 56.78 % relative humidity, and during the study were

71.1

− 75.09 ° F, and 35.88

− 42.83 % relative humidity

.

**Euthanasi a:**  At the end of the in

- life phase, the rats were euthanized by overexposure to carbon

dioxide.

3. 4 Test Chemical Preparation and Analysis

For inhalation exposure to [2

- 14 

### C]TBA/TBA (Studies A and B), 14 

### C TBA was added to an

appropriate amount of unlabel ed TBA for generation of the exposure atmosphere. The amount of

labeled and unlabeled TBA required was calculated based on the exposure concentration, the duration of

exposure, the number of animals, and the flow rate of air through the exposure tower.

For inhalation exposure to [2

- 14 

### C]TBA/TBA, [2

- 14 

### C]TBA was weighed into a tared flask with a

Teflon faced screw cap. The weight of the labeled chemical added was recorded. Unlabeled TBA was

added, and the weight added was recorded. The nominal specific activity of the TBA mixture will then be

calculated. This was verified by weighing an aliquot into a sealed flask, recording the weight added, and

adding solvent, and recording the weight added. Aliquots of the solution of TBA w ere placed in

scintillati on vials, and the weight added was recorded. The amount of solution added was calculated

based on the density of the solvent. Ultima GoldTM scintillation cocktail (Perkin Elmer) was added to the

scintillation vials, and the amount of radioactivity added was determined by liquid scintillation

spectroscopy (LSS). The specific activi ty of the labeled TBA w as then be calculated from the data

obtained.

The exposure atmosphere concentration was monitored using a c alibrated Miran IR detector

.

The c oncentration of TBA w as monitored at the inlet to the tower, and at the outlet . The stabi lity of TBA

under the conditions of administration was monitored by sampling the inlet of the exposure tower at the

end of the exposure. S amples of the exposure atmosphere were collected using a 10 ml syringe, and

bubbled through water for analysis by HPL C, and through methanol for analysis by GC

- MS.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 5 3. 5 Inhalation Exposure

A single nose

- only inhalation exposure to [2

- 14 

### C]TBA/TBA was conducted with a total of 8 rats for

Study A (4 rats) and Study B (4 rats) on June 1 st 

### , 2006. The radiolabeled TBA was drawn into a clean

syringe. The generation system consisted of a syringe containing the chemical with a syringe pump to

deliver the chemical to the air supply of the exposure chamber. The TBA exposure atmosphere was

generated by pumping liquid TBA into t he air stream flowing into a Cannon nose

- only exposure system.

The syringe pump and clean air flow rate were set such that a target concentration of 1750 ppm was

achieved. The exposure air flow rate delivered to each animal was at leas t 1.5 times the ani mal minute

ventilation rate. The air supply was controlled with an electronic mass flow meter to maintain a total air

flow of at least 12 air changes per hour. The generation and delivery system were placed in a chemical

hood to contain any TBA that m igh t leak from the system.

The exposure system (see Appendix E and Appendix F) consisted of a Cannon flow

- past nose

only exposure system containing 52 exposure ports

. This chamber is a dynamic non

- rebreathing system

that allows for the simultaneous exposure of up to 51 animals. The chamber is cylindrical in shape, and

is constructed in stainless steel. Unused ports were plugged with solid metal rods to reduce consumption

of the exposure material. The Cannon nose

- only chamber operated on a push

- pull basis. The sampling

lines and main exposure atmosphere delivery lines were Teflon. The incoming air for the exposure

system was filtered to eliminate the possibility of contamination in the air supply. The air supply

temperature and relative humidity were mai ntained between 64 to 79 degrees Fahrenheit and 30 to 70 %,

respectively. The chamber exhaust flow was adjusted to maintain a slight negative pressure during the

exposure to prevent TBA from entering the laboratory area. The chamber exhaust was filtered through a

disposable charcoal filter and disposed at the end of the exposure. Closed nose

- only tubes were used to

hold the test animals during inhalation exposures. The inhalation system was strategically placed in a

chemical hood to prevent any TBA fro m entering the laboratory.

3. 5 .1 Exposure Analysis

Prior to the inhalation exposure of rats, the performance of the inhalation system was verified to

ensure that the test atmosphere could be generated on the nose only tower to achieve the test

concentratio n over the duration of the exposure, and that the port to port variability was within accepted

limits. A report describing the preexposure characterization of the inhalation system is attached to this

report as Appendix E , and a report characterizing the e xposure of rats is attached as Appendix F.

3. 6 Collection of Samples

3. 6 .1 Excreta

In Study B, 4 rats (2

- 05, 2

- 06, 2

- 07, and 2

- 08) were placed in all

- glass metabolism cages for the

separate collection of urine, feces, exhaled volatiles, and exhaled CO 2



### . Urine was collected over dry ice

at 0

− 12, 12

− 24, 24

− 48, 48

− 72, 72

− 96, 96

− 120, 120

− 144, and 144

− 168 hours after termination ofRTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 6 exposure. Feces were collected over dry ice at 0

− 24, 24

− 48, 48

− 72, 72

− 96, 96

− 120, 120

− 144, and

144

− 168 hours after termination of exposure. Exhaled volatile organics were collected on a series of two

charcoal traps ( Product Number 226

- 16

, SKC Sorbent Tube, Anasorb CSC, Coconut Charcoal, 10 X 110

mm size, 2

- section, 200/800 mg sorbent, SKC Inc

, Eighty Four, PA ). Expired 14 

### CO 2 

### was collected in

1.0 N KOH at 0

− 1, 1

− 3, 3

− 5, 5

− 7, 7

− 12, 12

− 24, 24

− 48, 48

− 72, 72

− 96, 96

− 120, 120

− 144, and 144

− 168

hours after termination of exposure. At the end of excreta collection, the cage s were rinsed with water

and with ethanol , and combined

. The ri nses were analyzed for total radioactivity. The weight of urine

and/or feces collected for each sample interval was measured. Urine and feces were analyzed for total

radioactivity. Excreta not assayed within a day of collection were stored at approximat ely

- 20 ° C in the

dark.

3. 6 .2 Carcass

For Study A, t he amount of 14 

### C retained was determined by placing each rat in the nose

- only

restraint tube in a Tedlar gas bag. The bag was sealed, and CO 2 

### was pumped into the bag to euthanize

the rat. The gas from the gas bag was then forced through a charcoal filter trap, to determine the amount

of exhaled 14 

### C (the sample is designated as Exhaled VOC for Group 1 animals ) , and the carcass was

digested with 2N ethanolic NaOH . After sample digestion , the amount of r adioactivity in the carcass was

determined by LSS . Any feces in the nose

- only restraint tube was collected and the amount of

radioactivity in the feces sample was determined by LSS . The nose

- only tube was rinsed with water, and

the amount of 14 

### C containe d in the rinse was determined by LSS. The Tedlar gas bag was rinsed with

water, and the amount of 14 

### C contained in the rinse was determined by LSS.

For Study B, the carcasses were digested with 2N ethanolic NaOH . After sample digestion , the

amount of r adioactivity in the carcass was determined by LSS

.

3. 7 Analysis of Samples for Total Radioactivity

Ultima Gold TM scintillation cocktail was used in all determinations of radiochemical content.

Radioactivity was determined using a Packard 1900CA Liquid Scintillation Counter. Weights of samples

collected were recorded, and aliquots were prepared by weight for scintillation counting.

3. 7 .1 Excreta

Duplicate aliquots of urine (approximately 0.5 g) were analyzed directly (without solubilization or

bleachin g) for radiochemical content. Duplicate aliquots of urine were weighed into scintillation vials

containing scintillation cocktail. Feces were homogenized with an approximately equal mass of water.

The weight of the feces homogenate was determined, and d uplicate homogenate aliquots were weighed

into scintillation vials. After solubilization of the homogenate aliquo ts with Soluene

- 350TM (about 2 ml per

sample), scintillation cocktail was added to the vials, and the samples were analyzed for total radioacti vity

by LSS. Samples were bleached (by adding approximately 125 μ l of 70 % perchloric acid, and then

approximately 0.3 ml of 30% H 2 

### O 2 

### ) prior to addition of scintillation cocktail. Control samples of urine andRTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 7 feces w ere collected from unexposed animals an d analyzed as described above to determine background

counts.

3. 7 .2 Carcass

Carcasses were analyzed for total radioactivity following solubilization in 2N ethanolic NaOH

.

Duplicate samples of the solubilized carcass were analyzed. Solubilized samples wer e bleached (by

adding approximately 125 μ l of 70 % perchloric acid, and then approximately 0.3 ml of 30% H 2 

### O 2 

### ) prior to

addition of scintillation cocktail and analysis by LSS. Control samples of carcass were collected from

unexposed animals and analyzed as described above to determine background counts.

3. 7

. 3 Exhaled Breath Traps

Aliquots of 1.0 N KOH from the exhaled breath trap for CO 2 

### were analyzed by LSS after addition

of scintillation cocktail.

3. 7

. 4 Exhaled Volatiles Traps

Each charcoal trap was ex tracted by elution with dimethylformamide (DMF) to determine

absorbed radioactivity

. To ensure that the samples with large amounts of radioactivity were thoroughly

extracted, two extractions were performed on the VOC Trap 1 samples collected between 1 and 24 h.

For a single extraction, charcoal was transferred to a preweighed centrifuge tube and weighed. DMF (4.0

ml) was added, and the sample weighed. After vortexing for approximately 30 seconds, the samples

were centrifuged, and the supernatant was rem oved. R adioactivity in the DMF wash was measured by

LSS of duplicate aliquots. For samples with two extractions, charcoal was transferred to a preweighed

centrifuge tube and weighed. DMF (4.0 ml) was added, and the sample weighed. After vortexing for

a pproximately 30 seconds, the samples were centrifuged, and the supernatant was removed , and

transferred to a preweighed centrifuge tube labeled Exhaled VOC Trap 1 Sample

. The sample was

weighed, and duplicate aliquots were removed for analysis by LSS. Ad ditional DMF (4.0 ml) was added

to the tube containing the charcoal and residual DMF, and the extraction was repeated. The DMF was

removed following centri fug ation and radioactivity was determined in duplicate aliquots by LSS. For

samples of VOC Trap 1 collected at 1 h, a third extraction was performed, and the recovery of

radioactivity was evaluated prior to analysis of the remaining samples.

The sample designations used in the data tables correspond to the sample designations used in

the Debra Syste m, and are as follows:

Exhaled VOC Trap 1 First extract of the first VOC trap in series

VOC Trap 1 Extract 2 Second extract of the first VOC trap in series

Exhaled VOC Trap 2 Extract of the second VOC trap in seriesRTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 8 3. 8 HPLC Analysis of Urine Samples and Timed Fractions Collected from HPLC for

Radioactivity

Urine samples containing greater than 5% of the recovered radioactivity were selected for

analysis of metabolites by HPLC

. Aliquots of the selected urine samples were transferred to glass

centrifu ge tube s , and the samples were c entrifuge d at 3000 rpm for 5 minutes to remove particulate

matter. The supernatant was t ransfer red to a 1.5 ml HPLC vial. Aliquots ( 30 μl ) of urine were analyzed in

duplicate directly by LSS to determine amount of radioact ivity present in the 30 μ l aliquot.

A 30

- μl aliquot of urine was analyzed by HPLC/LSS as described below

. The HPLC

instrumentation consists of two Waters Model 515 Pumps, a Waters Model 717 plus Autosampler, an

IN/US Systems β

- RAM Model 3 radioactivity detector, and a Waters Model 410 refractive index (RI)

detector.

HPLC was conducted using a Waters Atlantis dC18 column, 4.6 mm i.d. x 25 cm, 5 μ particle

size, with a precolumn and an in

- line solvent filter. The isocratic mobile phase consist ed of 99 % water

and 1% acetonitrile. The mobile phase flow rate through the column was 1.0 ml/min, and 100% of the flow

passing through the 500

- μl lithium glass solid

- phase cell of the radioactivity d etector before passing

t hrough the refractive index detector. HPLC column effluent was collected in scintillation vials containing

scintillation cocktail, and quantitation of radioactivity was conducted using a Packard 1900CA liquid

scintillation counter.

A standard solution inc luding unlabeled TBA with metabolites of α

- hydroxyisobutyric acid and 2

-

methyl

- 1

- 2

- propanediol was used to conduct the performance verification for RI detector at the beginning

and end of analysis of all urine samples.

Incubation of urine samples with β

- glucuronidase was conducted by adding 15 mg of β

glucuronidase (Sigma

- Aldrich) to each incubation tube, and adding 50 μl of 0.2 M sodium acetate buffer

(pH 4.43) and 200 μl of urine . For one incubation, water was added instead of sodium acetate buffer, a nd

for one tube the β

- glucuronidase was boiled prior to addition of the urine. After addition of all

components, the samples were incubated for 12 hours at 37 o 

### C. This was followed by analysis by HPLC

with detection of radioactivity.

3. 9 Determination of TBA in Exhaled Breath Trap Extracts.

Aliquots of the DMF extracts of Exhaled Breath Traps were analyzed by GC with FID detection,

Standard solutions were prepared at concentrations of 0.01, 0.1, 1 and 10 μg/μl. Analysis was

conducted on an Agilent 6890 GC. Conditions for analysis were as follows:

Injection port split/splitless

Temperature 200 oCRTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 9 Split ratio 1.0

Carrier gas Helium

Flow rate 2.0 ml/min

Injection volume 1 μl

Detector FID, 250 oC

GC oven program

Initial t emperature 60 oC

Initial time 0.5 min

Temperature rate 5 oC/min

Final temperature 70 oC

Final time 2 min

Ramp A 100 oC/min

Final temperature A 200 oC

Final time A 1.2 min

Column DB 624 30m x 0.32 mm i.d. 1.8 um film thickness (Agilent technologies,

Wilmington, DE)

The peak area of TBA was integrated. A linear regression equation (y= bx+a) was obtained using

Prism software (version 4.03). The concentration TBA was back calculated by this equation.

Where: y= peak area of TBA

x = concentration of TBA

b = slope of the standard curve

a = y

- intercept of the standard curve

3.1 0 Data Collection and Reporting

Study data was coll ected and reported in the Debra TM s ystem version 5.5.10.72. This includes

data for pot weights, sample weights, homogenate weights and aliquot weights, and scintillation counting

data. Calculation s of sample data are described in Appendix G , and were reported with the Debra TM

s ystem

. I ndividual values for sample weights and radioactivity determinations are presented in Appendix

H. Data for radioactivity where the sample aliquot dpm values were below the background for the sample

were considered to be less than the limit of detection, a nd are reported as 0 dpm per aliquot. Data for all

samples that were above the limit of detection are reported, and included in calculations. However,

where the sample aliquot dpm values were less than three times the background for the sample, the

sampl e has been flagged with an asterisk in the S ummary T ables and Appendix tables.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 10 4.0 RESULTS

4.1 Analysis of Radiolabeled Test Chemical Identity and Purity

Information on the identity, purity, and stability of the test chemical is included in Appendix B

.

Prior to study initiation, the test chemical purity was determined by GC to be 99.55 ± 0.18 %. Following

completion of the study, test chemical purity was determined to be 99.71 ± 0.01 %

. The radiochemical

purity of 2

- 14 

### C TBA specified by the vendor was 98.994 % by HPLC, and was verified by HPLC at RTI

prior to the inhalation exposure of rats as 98.16 ± 0.27% ( Appendix C )

. A sample of the exposure

atmosphere was taken from the tower inlet to measure purity of the radiolabeled test chemical during th e

exposure. The purity of the radiolabeled material was determined to be 94.6 ± 2.4 %. Analysis of the

reference substances 2

- methyl

- 1,2

- propanediol and α

- hydroxyisobutyric acid is reported in Appendix D.

4.2 Test Chemical Formulation

The test chem ical was prepared by mixing unlabeled TBA with 2

- 14 

### C labeled TBA, and used

without further dilution. The specific activity of the formula tion is provided in Table 2, and was measured

as 18.57 μCi/mmol.

4. 3 Inhalation exposure

The inhalation exposure s ystem performance was evaluated and reported in Appendix E.

Concentrations of TBA were measured at the inlet and the exhaust, and were measured in open ports,

which represent the breathing zone of the rat. For the operation of the inhalation exposure sys tem, which

has a relatively short path length, the inlet port was considered to be a surrogate for the breathing zone of

the rat. The concentration at the inlet and exhaust were found to be in good agreement with and within

the acceptable range of variati on specified in the study protocol of ≤ 10% of the nominal concentration of

1750 ppm (Appendix E, Table 5, 1681 ± 73 ppm at the inlet, and 1722 ± 36 ppm at the exhaust).

Following equilibration, there was also good agreement at each timepoint between inle t and exhaust,

implying no loss in the system. Port to port variability was verified to be within the acceptable range of ≤

10% difference in concentration measurements taken at multiple locations (Appendix E, Table 3). To

make the required measurements of port to port variability, to decrease the time required for the Miran to

reach equilibrium, the air sampled at each port was diluted with house air, resulting in a lower

concentration measured at the port. A total flow of 200 mL/min consisting of 150 mL/min from the port

diluted with house air at 50 mL/min was delivered to the Miran detector. For measurement of within port

variation, a single port, designated the “ H ome P ort” (position 9) , was sampled repeatedly. Other ports,

numbered 1 through 8 wer e sampled once, with a sampling sequence of Home, 1, 2, 3, Home, 4, 5, 6,

Home, 7,8, and Home, Repeated measurements at the Home Port produced a within port coefficient of

variation of 1.16%, a between port coefficient of variation of 3.13%, and total por t coefficient of variation

of 3.34% . The measured port concentrations were less than the target concentration of 1750 ppmRTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 11 because house air was used to dilute the sample air to reduce the time necessary to reach equilibrium.

However, after correction fo r the dilution with house air, the port concentrations were 1766 ± 20 2 ppm,

and were 99.4 ± 7.2 % of the inlet port concentration.

The data for a six

- hour exposure of rats to a target concentration of 1750 ppm TBA is shown in

Table 2. The details of the inhalation exposure system are described in Appendix E, Inhalation Summary

Report for Setup and Evaluation of Inhalation System at RTI International and the details of the actual

exposure are reported in Appendix F

, Inhalation Summary Report: 14 

### C

- TBA / TBA Nose

- Only Inhalation

Exposure at RTI International

. The actual exposure concentration was 1724 ± 85 ppm (Table 2). The

design of the sampling at the inhalation tower inlet using the inlet Miran infrared spectrophotomer may

have led to artificially l ow concentration readings for the first two timepoints (Appendix F, Table 4, 1447

ppm at 32 minutes, and 1689 ppm at 62 minutes). This was caused by the large volume of the Miran

detector flow cell relative to the sampling flow rate to the inlet Miran of 200 ml/min. Excluding the first

two timepoints from the calcul ation results in an estimated concentration of 1752 ± 7 ppm

.

4.4 Radioactivity Retained Following Exposure

Four rats were euthanized immediately following inhalation exposure to [2

- 14 

### C]TBA/TBA ( Study A,

Group 1, rats 1

- 01, 1

- 02, 1

- 03, and 1

- 04) . The amount of radioactivity retained in the carcass was

determined by solubilization followed by scintillation counting. The total radioactivity retained and the

estimated dose in mg/kg are presented in Table 3. The amount of radioactivity retained in Group 1

ranged from approximately 22 to 2 8 μCi. The estimated dose was 532 ± 38 mg/kg. This estimate of

dose included radioactivity recovered in carcass, from washing the bag used to euthan ize the rat with

CO 2 

### (Transfer Bag Rinse) , a VOC trap that was used to trap any exhaled volatiles while the rat was in the

transfer bag, the nose only tube wash, and feces recovered from the nose only tube. The vast majority

(99%) of the radioactivity w as recovered in the carcass (Table 4).

4. 5 Excretion of Radioactivity

Four rats were placed in metabolism cages for collection of urine and feces and exhaled breath

immediately following the inhalation exposure to [2

- 14 

### C]TBA/TBA ( Study B, Group 2, Rats 2

- 0 5 , 2

- 0 6, 2

-

0 7 , and 2

- 0 8 )

. Transfer of the rats from the inhalation tower to the metabolism cage was conducted with

the rat retained in the nose

- only tube contained within a polyethylene bag. Following transfer of the rat to

the metabolism cage, radio activity retained in the bag was recovered by washing with water. The nose

only tube was washed and any feces were collected for digestion and scintillation counting. The rats

were maintained in the metabolism cages for 7 days following the end of the ex posure. On the morning

of the last da y in metabolism cages, one rat was found dead, as a result of asphyxiation because of

clogging of one of the sinters in the exhaled CO 2 

### traps, which had restricted air flow (Rat 2

- 08). Samples

from this rat were coll ected, and analyzed for the recovery of radioactivity. The total recovery ofRTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 12 radioactivity from all of the samples collected from rats in group 2 is presented in Table 3, and ranged

from approximately 22 to 28 μCi. The estimated dose was 510 ± 40 mg/kg ( Table 3)

.

The amount of radioactivity in urine

, feces , exhaled VOCs, and CO 2 

### expressed as a percentage

of the total radioactivity recovered is presented in Table 5

. Urinary excretion was the primary route of

elimination , with 7 1% of the recovered radio activity excreted in the urine, and 2 % excreted in feces.

Exhaled volatiles (VOC traps) accounted for the second largest fraction of the excreted radioactivity, with

the majority of the VOC radioactivity retained on the first charcoal trap in a series of t wo. A second

extract of the first trap recovered additional radioactivity, with approximately 25 % (the first and second

extract combined) of the recovered radioactivity in the first trap. The recovery of the radioactivity from

the exhaled VOC traps wa s investigated by extracting the first VOC traps from the 0

- 1 hr timepoint for rats

2

- 01, 2

- 02, 2

- 03, and 2

- 04 three times with successive aliquots of DMF. The weight of DMF added was

recorded, and the weight of DMF extract removed was also recorded. The amount of radioactivity

recovered in each extract was calculated based on the amount of radioactivity removed, and on the

amount of radioactivity in the total volume of DMF. Compared with three successive extracts, it was

determined that a single extract recovered approximately 82.5 ± 5.9 % of the radioactivity, and two

extracts recovered 99.8 ± 1.1 % of the radioactivity. For the samples obtained at times up to 24 hours,

two extracts were applied to each of the VOC trap 1 samples. For those samples of VOC trap 1 obtained

after 24 hours, a single extraction with DMF was conducted. Exhaled 14 

### CO 2 

### accounted for approximately

0.5 % of the recovered radioactivity.

Radioactivity in urine was excreted primarily in the first 48 hours (Table 6) , with 37%, 22%

, 9 .3 %

and 1.9% excreted at 12, 24, 48 , and 72 hours, respectively. Less than 1% of the radioact ivity was

recovered in urine at 96, 120, 144, and 168 hours. In feces (Table 7) , approximately 0.9 % of the

recovered activity was found in the 0

- 24 h samples, w hich contained the largest amount of fecal

radioactivity.

Radioactivity in the first CO 2 

### trap was excreted primarily in the first 48 hours (Table 8). Many of

the samples collected after 48 hours contained levels of radioactivity below 3 times backgroun d

. Little

r adioactivity was recovered in the second CO 2 

### trap (Table 9). Many of the samples collected contained

levels of radioactivity that were below 3 times background

.

The majority of the radioactivity retained on the charcoal traps for exhaled vola tiles was retained

on the first charcoal trap of two in series in the first 24 hours (Table 10). For each of the VOC Trap 1

samples collected between 0 and 24 hours, a second extract was performed. Data from these analyses

are presented in Table 11. Fo r the cumulative total, a pproximately 14% was recovered in the first extract

(0

- 168h) , and 1 1 % in the second extract (0

- 24h)

. Very little of the dose was trapped in the VOC Trap 2

samples, with one exception. For rat 2

- 05, the 48

- 72 h VOC trap 2 sample contained 0.113 % of the

radioactivity, whereas the 48

- 72 h VOC trap 1 sample contained only 0.000%. Reanalysis of these

samples suggested that the traps were switched in order during collection.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 13 The cumulative excretion of radioactivity (Table 1 3 ) shows that approximately 95% of the activity

was recovered by 48 hours following exposure.

4. 6 TBA in Exhaled VOC Traps

To evaluate the pulmonary excretion of TBA, DMF extracts of the charcoal exhaled VOC traps

were analyzed by GC

- FID for quantitation of T BA. This analysis was confin ed to the first VOC trap, since

the amount of radioactivity recovered from VOC Trap 2 was extremely low. The concentration of TBA

was highest in the samples collected at 1, 3, 5, 7, and 12 hours (Table 1 4 )

. The concentration of TBA in

the extracts fell rapidly by 24 hours, and was not detectable in all but one sample by 96 hours (Rat 2

- 08,

Exhaled VOC Trap 1, 168 hr, 0.013 mg/g)

.

Comparison of the concentration of radioactivity (mg equiv. TBA/g DMF) vs. the concentration of

TBA measured in each DMF extract provided an estimate of the percentage of TBA in the exhaled VOC

trap. The percentage as TBA was high initially at 84 % in the first hour following exposure, dropping to a

low of approximately 28 % at 48 hours.

4. 7 Excretion of TBA and Metabolites in Urine

HPLC analysis of urine was limited to samples containing greater than 5% of the total recovered

radioactivity. HPLC analysis of 14 

### C TBA metabolites in urine revealed that little of the radioactivity was

due to TBA itself. Typical chromatograms of urine are shown in Figure 1. The retention times of TBA and

standards for 2

- methyl

- 1,2

- propanediol and α

- hydroxyisobutyric acid were established by

chromatography with refractive index detection (Figure 2). The percenta ge of the total radioactivity that

was present as unchanged 14 

### C TBA in urine was extremely low at all time points measured (Table 1 6 ),

with the highest levels observed at 12 h following exposure, with 14 

### C TBA accounting for 4.4% of the

urinary radioactivit y. At 24 hours, 14 

### C TBA was detected in urine from two of four rats, and by 48 hours

none was detected in any of the urine samples of exposed animals . Three additional radiolabeled peaks

( metabolite s) were observed. One of these peaks (Peak 3) co

- eluted with 2

- methyl

- 1,2

- propanediol.

Peak 2 was removed after incubation with β

- glucuronidase, producing TBA and a broad peak at a

retention time of approximately 15 minutes (Figure 3) . Incubation with acid caused movement of peaks 1

and 2 with the appearance of a new peak that was consistent with the r etention time of α

- methyl

isobutyric acid.

5.0 CONCLUSION

The objectives of the study were successfully met. A nose

- only system for the inhalation

exposure of rats to 14C TBA was implemented. Male rats we re exposed to 14 

### C TBA/TBA, and the

recovery of radioactivity was determined immediately following exposure and over a 7

- day period with

collection of urine, feces, exhaled volatiles and 14 

### C CO 2



### . Comparison of the exposure of rats to 1750 ppm

[2

- 14 

### C]TBA/TBA euthanized immediately following exposure (532 ± 38 mg/kg) with that of rats euthanizedRTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 14 following 7 days of collection of excreta and exhaled breath ( 510 ± 40 mg/kg ) yielded values that were

very similar. This suggested that there were no significant los ses of radioactivity from the various sample

types during processing of radioactivity.

Urinary excretion was the major route of excretion, followed by exhalation as volatile components.

Radioactivity was rapidly excreted in urine (71% of the recovered rad ioactivity, Table 6) and in exhaled

breath as volatile components (25% of the recovered radioactivity, Tables 10 and 11). The cumulative

excretion of radioactivity was 86% and 96% within 24 and 48 hours, respectively, of the end of the

exposure. At seven days, only 0.6% was recovered in the carcass.

The determination of unchanged TBA and metabolites in urine and exhaled breath traps indicated

that TBA is extensively metabolized. Unchanged TBA was detected as a substantial component in

exhaled breath (r anging from mean values of 95

– 49% in the first 24 h following exposure)

, and as a

minor component in urine at 12 (mean value of 4.4 %) and 24 hours (0.5 %, mean of two animals)

. In

urine collected at 12 h, three metabolites and 14 

### C TBA were detected by HPLC with radioactivity

detection. By 24 hours, 14 

### C TBA was detected in 2/4 urine samples, and by 48 h was not detected. The

three metabolites were tentatively identified as 2

- methyl

- 1,2

- propanediol, TBA glucuronide, and a

glucuronide of α

- hydroxyisobuty ric acid.

Analysis of the drinking water for the presence of oxygenates during the study revealed the

presence of a single measurement of tertiary butyl alcohol (2.2 μ g/L) that was above the reporting limit

(1.0 μ g/L). The detected concentration is consi derably below the interim drinking water standard le vel of

12 μg/L developed by the California Office of Environmental Health Hazard Assessment (OEHHA) (Office

of Environmental Health Hazard Assessment 1999)

. Estimati on of the consumption of water from rats

administered TBA (NTP 1995) in the drinking water (approximately 22 ml/day) suggests that the

consumption of unlabeled TBA was at most 0.3388 μ g (2.2 μ g/L x 0.022 L/day x 7 days).

6.0 REFERENCES

Baker, R. C., Sorens en, S. M. and Deitrich, R. A. (1982). The in vivo metabolism of tertiary butanol by

adult rats. Alcohol Clin Exp Res 6 :247

- 51.

Borghoff, S. J., Prescott, J. S., Janszen, D. B., Wong, B. A. and Everitt, J. I. (2001). alpha 2u

- Globulin

nephropathy, renal cel l proliferation, and dosimetry of inhaled tert

- butyl alcohol in male and female F

- 344

rats. Toxicol Sci 61 :176

- 86.

Faulkner, T. P. and Hussain, A. S. (1989). The pharmacokinetics of tertiary butanol in C57BL/6J mice.

Res Commun Chem Pathol Pharmacol 64 :31

- 9.

Mahler, J. (1997). NTP technical report on toxicity studies of t

- butyl alcohol (CAS No. 75

- 65

- 0).

Administered by inhalation to F344/N rats and B6C3F1 mice. Toxic Rep Ser:1

- 56, A1

- D9.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 15 NTP (1995). NTP Toxicology and Carcinogenesis Studies of t

- Butyl Alc ohol (CAS No. 75

- 65

- 0) in F344/N

Rats and B6C3F1 Mice (Drinking Water Studies). Natl Toxicol Program Tech Rep Ser 436 :1

- 305.

Office of Environmental Health Hazard Assessment (1999). Expedited Evaluation of Risk Assessment

for Tertiary Butyl Alcohol in Drin king Water.

Poet, T. S., Valentine, J. L. and Borghoff, S. J. (1997). Pharmacokinetics of tertiary butyl alcohol in

male and female Fischer 344 rats. Toxicol Lett 92 :179

- 86.

US EPA (1988). Recommendations for and documentation of biological values for use in risk

assessment . Environmental Criteria and Assessment Office, Cinc innati, Ohio. EPA/600/6

- 87/008

.

Williams, T. M. and Borghoff, S. J. (2001). Characterization of tert

- butyl alcohol binding to alpha2u

globulin in F

- 344 rats. Toxicol Sci 62 :228

- 35.

7. 0 RECORDS AND REPORTS

The following will be maintained in the record:

a ) Protocol and any amendments

b ) Animal receipt records

c ) Quarantine records

d ) Temperature and humidity records for the treatment rooms

e ) Animal research facility room logs

f ) Feed and water analysi s for contaminants

g ) Randomization records

h ) Test chemical receipt, storage, and use records

i ) Balance calibration log references

j ) Correspondences

k ) All other raw data and documentation

Upon acceptance of the audited draft report by the Sponsor, a final report will be issued.

8.0 STORAGE OF RECORDS A ND BIOLOGICAL SAMPLE S

A copy of the final report and the records for this study, including all raw data, will be retained in

the RTI Archives, under the responsibility of the RTI Quality Assurance Unit, for the lengt h of time

specified in the appropriate regulations.

Biological specimens that remain stable for reanalysis and samples of nonradiolabeled and

radiolabeled TBA will be stored at RTI in a secured area by the RTI project number for up to two years

after the d ate of submission of the final report.

The Sponsor will be notified in writing when the RTI retention time has expired.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 16 9.0 REGULATORY COMPLIANC E

These studies were performed in compliance with the EPA Good Laboratory Practices (GLP)

Standards for Inhala tion Exposure Health Effects Testing, 40 CFR part 79, subpart F § 79.60. Deviations

from the approved protocol are documented in Appendix A

.

10.0 STUDY PARTICIPANTS

RTI International

Study Director: Timothy R. Fennell, Ph.D.

Veterinarian: J.S. Scott

- Emuakpor , D.V.M.

Animal Facility Supervisor:

Laboratory Staff: N. F. Gaudette, BS

Y. Hong, BS

R. W. Snyder, MS

M. P. Gower

Secretary: K. G. Ancheta

Quality Assurance Reviewer: K. Collier

C. Ingalls

CIIT Centers for Health Research

Principal In vestigator:: R Arden James.

Laboratory Staff: K. Roberts

Quality Assurance Reviewer: P. O’Brien Pommerleau

A. MatroneRTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 17 Table 1

. M ale Fischer 344 Rats Used on the Study

Group Subject Species Strain Subj Wt a



### Group 1 Study A 1

- 01 Rat Fischer 344 192.73 g

Group 1 Study A 1

- 02 Rat Fischer 344 201.00 g

Group 1 Study A 1

- 03 Rat Fischer 344 196.32 g

Group 1 Study A 1

- 04 Rat Fischer 344 187.85 g

Group 2 Study B 2

- 05 Rat Fischer 344 193.42 g

Group 2 Study B 2

- 06 Rat Fischer 344 203.17 g

Group 2 Study B 2

- 07 Rat Fischer 344 203.48 g

Group 2 Study B 2

- 08 Rat Fischer 344 202.00 g

Group 3 Study B BKG b 

### 3

- 09 Rat Fischer 344 187.70 g

Group 3 Study B BKG 3

- 10 Rat Fischer 344 206.45 g

Group 4 Study A BKG b 

### 4

- 11 Rat Fischer 344 195.31 g

a 

### Body weight on the day of exposure

b 

### Animals used in Group 3 and Group 4 were not exposed to [2

- 14 

### C]TBA/TBA , and provided samples for

determination of background only.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 18 Table 2

. Exposure Conditions and Concentration of [2

- 14 

### C ]TBA/ TBA.

Analysis results

Treatment name: TBA 1750 Nose

- Only Inhalation

Dpm/g of dose prep: 556338044.1 dpm/g

Molecular weight: 74.12

Specific Activity : 0.2506 μCi/mg

Exposure Summary

Target (ppm)

Number of Open Ports 1750

12

ACC 1



### - Inlet Mean 1724

(ppm) Std Dev 85

No. of Data Points 2 

### 13

ACC

- Exhaust Mean 1581

(ppm) Std Dev 40

No. of Data Points 2 

### 13

Exposure Mean 72.8

Temperature Std Dev 0.5

(°F) No. of Data Points 2 

### 13

Exposure Mean 47.6

Relative Humidity Std Dev 0.5

(%) No. of Data Points 2 

### 13

Exposure Mean

- 0.05

Static Pressure Std Dev 0.00

(in H 2 

### 0) No. of Data Points 2 

### 13

1 

### ACC Analytical Chamber Concentration

2 

### Data points were collected during the 6

- h inhalation exposure.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 19 Table 3

. Total Recovery of Radioactivity following Exposure to 1750 ppm [2

- 14 

### C]TBA/TBA

Rat Total DPM

Recovered Total

μ Ci Dose

(mg) Bodyweight

(g) Dose

(mg/kg) Group

Mean

Dose

(mg/kg) SD

1

- 01 59,110,333.86 26.6263 106.25 192.73 551.3 531.68 38.15

1

- 02 60,334,744.16 27.1778 108.45 201 539.6

1

- 03 61,168,290.20 27.5533 109.95 196.32 560.0

1

- 04 49,730,780.50 22.4013 89.39 187.85 475.9

2

- 05 49,473,192.23 22.2852 88.93 193.42 459.8 509.56 40.30

2

- 06 56,087,309.04 25.2646 100.82 203.17 496.2

2

- 07 60,071,898.65 27.0594 107.98 203.48 530.7

2

- 08 61,989,781.11 27.9233 111.42 202 551.6RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 20 Table 4

. Radioactivity in Group 1 Study A Rats Following Exposure to 1750 ppm [2

- 14 

### C]TBA/TBA , Expressed as a Percentage of the

Total R adioactivity Recovered

Group 1 Study

A Group 1 Study

A Group 1 Study

A Group 1 Study

A Group 1 Study

A Group 1 Study

A

Sample 1

- 01 1

- 02 1

- 03 1

- 04 Mean SD

Feces 0.216 0.041 0.244 1.670 0.543 0.757

Transfer Bag Rinse 0.032 * 0.005 0.561 0.033 0.158 0. 269

Carcass Digest 99.4 69 99.6 23 98.7 65 96.9 18 98.69 4 1.241

Exhaled VOC 1 

### 0.002 0.001 0.004 0.016 0.006 0.007

Nose Only Tube Rinse 0.281 0.329 0.426 1.362 0.600 0.512

Total 100.00 100.00 100.00 100.00 100.000 0.000

* Value is below 3 x background for the sample.

1 

### Exhaled VOC represents the charcoal trap attached to the Tedlar bag used for euthanizing the rats.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 21 Table 5

. Radioactivity in Excreta and Carcass following E xposure to 1750 ppm [2

- 14 

### C]TBA/TBA (Group 2 , Study B ) Expr essed as a

Percentage of the Total Radioactivity Recovered

Group 2 Study B Group 2 Study B Group 2 Study B Group 2 Study B Group 2 Study B Group 2 Study B

Male Male Male Male

Sample 2

- 05 2

- 06 2

- 07 2

- 08 Mean SD

Urine 74.113 70.499 69.858 69.479 70 .987 2.126

Feces 1.293 1.850 1.264 2.550 1.739 0.604

CO2 Trap 1 0.571 0.540 0.570 0.537 0.555 0.019

CO2 Trap 2 0.020 0.032 0.043 0.051 0.037 0.013

Exhaled VOC Trap 1 1 

### 12.402 14.420 15.042 14.894 14.190 1.221

VOC Trap 1 Extract 2 2 

### 10.051 11.181 12.087 11.429 11.187 0.848

Exhaled VOC Trap 2 0.114 0.005 0.006 0.002 0.032 0.055

Nose Only Tube Rinse 0.698 0.247 0.237 0.184 0.342 0.239

Transfer Bag Rinse 0.018 0.032 * 0.009 0.014 0.018 0.010

Carcass Digest 0.579 0.784 0.622 0.510 0.624 0.116

Cage Rinse 0 .139 0.409 0.263 0.350 0.290 0.118

Total 100.00 100.00 100.00 100.00 100.000 0.000

* Value is below 3 x background for the sample.

1 

### The first extract of the first VOC trap in series

2 

### The second extract of the first VOC trap in seriesRTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 22 Table 6

. Radioactivity in Urine following E xposure to 1750 ppm [2

- 14 

### C]TBA/TBA (Group 2 , Study B ) Expressed as a Percentage of the

Total Radioactivity Recovered

Group 2 Study B Group 2 Study B Group 2 Study B Group 2 Study B Group 2 Study B Grou p 2 Study B

Male Male Male Male

2

- 05 2

- 06 2

- 07 2

- 08 Mean SD

Urine 0

- 12 h 46.743 34.991 29.371 36.637 36.936 7.240

Urine 12 h

- 24 h 18.627 19.231 26.677 22.963 21.875 3.732

Urine 24 h

- 48 h 6.700 12.137 10.677 7.662 9.294 2.542

Urine 48 h

- 72 h 1.492 2.590 2.077 1.407 1.891 0.553

Urine 72 h

- 96 h 0.284 0.548 0.580 0.479 0.473 0.133

Urine 96 h

- 120 h 0.128 0.294 0.247 0.205 0.218 0.070

Urine 120 h

- 144 h 0.085 0.387 0.159 0.066 0.174 0.147

Urine 144 h

- 168 h 0.054 0.321 0.069 0.061 0.126 0.130

Total 74.113 70.499 69.858 69.479 70.987 2.126

* Value is below 3 x background for the sample.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 23 Table 7

. Radioactivity in Feces following E xposure to 1750 ppm [2

- 14 

### C]TBA/TBA (Group 2 , Study B ) Expressed as a Perce ntage of the

Total Radioactivity Recovered

Group 2 Study B Group 2 Study B Group 2 Study B Group 2 Study B Group 2 Study B Group 2 Study B

Male Male Male Male

2

- 05 2

- 06 2

- 07 2

- 08 Mean SD

Feces 0 h 0.126 0.266 0.091 1.034 0.379 0.443

Feces 0 h

- 24 h 0.929 0.952 0.845 0.800 0.881 0.071

Feces 24 h

- 48 h 0.134 0.206 0.221 0.233 0.199 0.044

Feces 48 h

- 72 h 0.065 0.056 0.045 0.089 0.064 0.019

Feces 72 h

- 96 h *0.017 0.063 0.035 0.082 0.049 0.029

Feces 96 h

- 120 h *0.005 *0.007 0.013 *0. 008 0.008 0.003

Feces 120 h

- 144 h *0.009 0.013 *0.008 0.262 0.073 0.126

Feces 144 h

- 168 h *0.008 0.286 *0.007 0.042 0.086 0.135

Total 1.293 1.850 1.264 2.550 1.739 0.604

* Value is below 3 x background for the sample.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 24 Table 8

. Radioactivity in CO2 Trap 1 following exposure to 1750 ppm 14 

### C TBA (Group 2 , Study B ) Expressed as a Percentage of the

Total Radioactivity Recovered

Group 2 Study B Group 2 Study B Group 2 Study B Group 2 Study B Group 2 Study B Group 2 Stud y B

Male Male Male Male

2

- 05 2

- 06 2

- 07 2

- 08 Mean SD

CO2 Trap 1 0

- 1 h 0.051 0.044 0.035 0.034 0.041 0.008

CO2 Trap 1 1 h

- 3 h 0.061 0.057 0.053 0.050 0.055 0.005

CO2 Trap 1 3 h

- 5 h 0.058 0.051 0.043 0.043 0.049 0.007

CO2 Trap 1 5 h

- 7 h 0.042 0.045 0.047 0.042 0.044 0.003

CO2 Trap 1 7 h

- 12 h 0.083 0.086 0.098 0.090 0.089 0.007

CO2 Trap 1 12 h

- 24 h 0.163 0.141 0.205 0.192 0.175 0.029

CO2 Trap 1 24 h

- 48 h 0.062 0.069 0.052 0.057 0.060 0.007

CO2 Trap 1 48 h

- 72 h *0.022 *0.023 *0. 017 *0.006 0.017 0.008

CO2 Trap 1 72 h

- 96 h *0.013 *0.010 *0.008 *0.011 0.011 0.002

CO2 Trap 1 96 h

- 120 h *0.008 *0.009 *0.005 *0.006 0.007 0.002

CO2 Trap 1 120 h

- 144 h *0.003 *0.004 * 0.002 *0.004 0.003 0.001

CO2 Trap 1 144 h

- 168 h *0.005 *0.00 2 *0.003 *0.002 0.003 0.002

Total 0.571 0.540 0.570 0.537 0.555 0.019

* Value is below 3 x background for the sample.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 25 Table 9

. Radioactivity in CO2 Trap 2 following exposure to 1750 ppm [2

- 14 

### C]TBA/TBA (Group 2 , Study B ) Expr essed as a Percentage of

the Total Radioactivity Recovered

Group 2 Study B Group 2 Study B Group 2 Study B Group 2 Study B Group 2 Study B Group 2 Study B

Male Male Male Male

2

- 05 2

- 06 2

- 07 2

- 08 Mean SD

CO2 Trap 2 0

- 1 h *0.001 * 0.000 *0. 003 * 0.000 0.001 0.001

CO2 Trap 2 1 h

- 3 h * 0.001 *0.002 * 0.001 * 0.000 0.001 0.001

CO2 Trap 2 3 h

- 5 h * 0.000 * 0.001 *0.003 *0.003 0.002 0.001

CO2 Trap 2 5 h

- 7 h * 0.000 * 0.003 * 0.000 * 0.000 0.001 0.001

CO2 Trap 2 7 h

- 12 h * 0.000 * 0.002 * 0.000 * 0. 000 0.001 0.001

CO2 Trap 2 12 h

- 24 h * 0.002 *0.002 *0.002 *0.003 0.002 0.000

CO2 Trap 2 24 h

- 48 h *0.009 *0.011 *0.019 *0.016 0.013 0.005

CO2 Trap 2 48 h

- 72 h *0.005 *0.006 *0.006 *0.020 0.009 0.007

CO2 Trap 2 72 h

- 96 h * 0.000 *0.003 *0.003 *0. 004 0.002 0.002

CO2 Trap 2 96 h

- 120 h *0.001 * 0.000 *0.002 *0.003 0.001 0.001

CO2 Trap 2 120 h

- 144 h * 0.002 *0.001 *0.003 * 0.002 0.002 0.001

CO2 Trap 2 144 h

- 168 h * 0.000 * 0.001 * 0.001 * 0.001 0.001 0.000

Total 0.020 0.032 0.043 0.051 0.037 0.013

* Value is below 3 x background for the sample.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 26 Table 10

. Radioactivity in Exhaled VOC Trap 1 following exposure to 1750 ppm 14 

### C TBA (Group 2 , Study B ) Expressed as a Percentage

of the Total Radioactivity Recovered

Group 2 Study

B Group 2 Study

B Group 2 Study

B Group 2 Study

B Group 2 Study

B Group 2 Study

B

Male Male Male Male

2

- 05 2

- 06 2

- 07 2

- 08 Mean SD

Exhaled VOC Trap 1 0

- 1 h 2.159 2.688 2.415 3.147 2.602 0.423

Exhaled VOC Trap 1 1 h

- 3 h 2.443 2.446 2. 229 2.561 2.420 0.138

Exhaled VOC Trap 1 3 h

- 5 h 2.719 2.479 2.287 2.601 2.521 0.185

Exhaled VOC Trap 1 5 h

- 7 h 2.209 2.061 2.415 2.078 2.191 0.164

Exhaled VOC Trap 1 7 h

- 12 h 2.129 3.011 3.730 3.072 2.986 0.657

Exhaled VOC Trap 1 12 h

- 24 h 0.4 49 0.690 1.266 0.877 0.820 0.345

Exhaled VOC Trap 1 24 h

- 48 h 0.184 0.757 0.560 0.222 0.431 0.275

Exhaled VOC Trap 1 48 h

- 72 h 0.000 0.154 0.039 0.146 0.085 0.077

Exhaled VOC Trap 1 72 h

- 96 h 0.036 0.046 0.030 0.055 0.042 0.011

Exhaled VOC Trap 1 96 h

- 120 h 0.038 0.035 0.027 0.037 0.034 0.005

Exhaled VOC Trap 1 120 h

- 144 h 0.019 0.027 0.025 0.029 0.025 0.005

Exhaled VOC Trap 1 144 h

- 168 h 0.018 0.027 0.018 0.067 0.033 0.024

Total 12.402 14.420 15.042 14.894 14.190 1.221RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 27 Table 11

. Radioactivity in VOC Trap 1 Extract 2 following E xposure to 1750 ppm [2

- 14 

### C]TBA/TBA (Group 2 , Study B ) Expressed as a

Percentage of the Total Radioactivity Recovered

Group 2

Study B Group 2

Study B Group 2

Study B Group 2

Study B Group 2

Study B Group 2

Study B

Male Male Male Male

2

- 05 2

- 06 2

- 07 2

- 08 Mean SD

VOC Trap 1 Extract 2 0

- 1 h 1.922 2.348 2.414 2.384 2.267 0.232

VOC Trap 1 Extract 2 1 h

- 3 h 2.059 2.135 2.058 2.478 2.183 0.200

VOC Trap 1 Extract 2 3 h

- 5 h 2.320 2.179 2.165 2.326 2.247 0.088

VOC Trap 1 Extract 2 5 h

- 7 h 1.459 1.469 1.737 1.508 1.543 0.131

VOC Trap 1 Extract 2 7 h

- 12 h 1.919 2.536 2.925 2.124 2.376 0.447

VOC Trap 1 Extract 2 12 h

- 24 h 0.373 0.514 0.788 0.608 0.571 0.174

Total 10.0 51 11.181 12.087 11.429 11.187 0.848

* Value is below 3 x background for the sample.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 28 Table 12

. Radioactivity in Exhaled VOC Trap 2 following E xposure to 1750 ppm [2

- 14 

### C]TBA/TBA (Group 2 , Study B ) Expressed as a

Percentage of the Total Radioactivity Recovered

Group 2 Study

B Group 2 Study

B Group 2 Study

B Group 2 Study

B Group 2 Study

B Group 2

Study B

Male Male Male Male

2

- 05 2

- 06 2

- 07 2

- 08 Mean SD

Exhaled VOC Trap 2 0

- 1 h * 0.000 * 0.000 * 0.000 * 0.000 0.000 0.000

Exhaled VOC Trap 2 1 h

- 3 h * 0.000 * 0.000 0.000 *0.000 0.000 0.000

Exhaled VOC Trap 2 3 h

- 5 h * 0.000 * 0.000 * 0.000 *0.000 0.000 0.000

Exhaled VOC Trap 2 5 h

- 7 h * 0.000 * 0.000 * 0.000 * 0.000 0.000 0.000

Exhaled VOC Trap 2 7 h

- 12 h * 0.000 * 0. 000 * 0.000 * 0.000 0.000 0.000

Exhaled VOC Trap 2 12 h

- 24 h * 0.000 * 0.000 0.005 0.000 0.001 0.002

Exhaled VOC Trap 2 24 h

- 48 h 0.001 0.001 * 0.000 *0.000 0.000 0.000

Exhaled VOC Trap 2 48 h

- 72 h 0.113 0.001 * 0.000 *0.000 0.028 0.056

Exhaled VOC Tra p 2 72 h

- 96 h * 0.000 0.001 * 0.000 0.001 0.000 0.001

Exhaled VOC Trap 2 96 h

- 120 h * 0.000 0.001 * 0.000 0.001 0.000 0.000

Exhaled VOC Trap 2 120 h

- 144 h 0.000 0.001 0.000 0.000 0.000 0.000

Exhaled VOC Trap 2 144 h

- 168 h * 0.000 * 0.000 * 0.000 *0.000 0.000 0.000

Total 0.114 0.005 0.006 0.002 0.032 0.055

* Value is below 3 x background for the sample.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 29 Table 13

. Cumulative Recovery of Radioactivity in Excreta and Carcass following Exposure to 1750 ppm [2

- 14 

### C]TBA/TBA (G roup 2,

Study B) Expressed as a Percentage of the Total Radioactivity Recovered

Group 2 Study

B Group 2 Study

B Group 2 Study

B Group 2 Study

B Group 2 Study

B Group 2 Study

B

Male Male Male Male

Timepoint 2

- 05 2

- 06 2

- 07 2

- 08 Mean SD

0 h 0.842 0.545 0.328 1.231 0.737 0.391

1 h 4.974 5.624 5.192 6.796 5.647 0.813

3 h 9.536 10.262 9.533 11.885 10.304 1.108

5 h 14.633 14.970 14.028 16.856 15.122 1.220

7 h 18.343 18.545 18.228 20.484 18.900 1.064

12 h 69.217 59.168 54.353 62.407 61.286 6.237

2 4 h 89.758 80.695 84.138 87.848 85.610 4.022

48 h 96.838 93.865 95.649 96.022 95.593 1.255

72 h 98.508 96.667 97.809 97.664 97.662 0.759

96 h 98.829 97.325 98.454 98.281 98.222 0.640

120 h 98.995 97.655 98.741 98.524 98.479 0.582

144 h 99.099 98.083 9 8.926 98.881 98.747 0.453

168 h 99.889 99.911 99.898 99.912 99.902 0.011

Total 99.889 99.911 99.898 99.912 99.902 0.011RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 30 Table 14

. TBA Measured by GC in DMF Extracts of VOC Trap 1 1 

### following Exposure to 1750 ppm [2

- 14 

### C]TBA /TBA

TBA Concentration (mg/g)

Sample Timepoint 2

- 05 2

- 06 2

- 07 2

- 08 Mean SD

Exhaled VOC Trap 1 1 h 0.796 0.810 0.843 1.149 0.899 0.167

Exhaled VOC Trap 1 3 h 0.638 0.680 0.751 0.902 0.743 0.116

Exhaled VOC Trap 1 5 h 0.697 0.652 0.649 0.806 0.701 0.0 73

Exhaled VOC Trap 1 7 h 0.562 0.563 0.768 0.635 0.632 0.097

Exhaled VOC Trap 1 12 h 0.524 0.786 1.191 0.999 0.875 0.287

Exhaled VOC Trap 1 24 h 0.075 0.099 0.247 0.218 0.159 0.086

Exhaled VOC Trap 1 48 h 0.011 0.083 0.011 0.024 0.032 0.034

Exhaled V OC Trap 1 72 h 0.014 0.021 <LOQ 0.028 0.021 0.007

Exhaled VOC Trap 1 96 h <LOQ <LOQ <LOQ <LOQ

- 2



### -

Exhaled VOC Trap 1 120 h <LOQ <LOQ <LOQ <LOQ

- -

Exhaled VOC Trap 1 144 h NA <LOQ <LOQ <LOQ

- -

Exhaled VOC Trap 1 168 h NA <LOQ NA 0.013

- -

VOC Trap 1 Extract 2 1 h 0.299 0.431 0.465 0.465 0.415 0.079

VOC Trap 1 Extract 2 3 h 0.329 0.377 0.408 0.523 0.409 0.082

VOC Trap 1 Extract 2 5 h 0.344 0.371 0.414 0.482 0.403 0.060

VOC Trap 1 Extract 2 7 h 0.243 0.269 0.322 0.308 0.286 0.036

VOC Trap 1 Extract 2 12 h 0.310 0.474 0.569 0.430 0.446 0.108

VOC Trap 1 Extract 2 24 h 0.039 0.082 0.130 0.100 0.088 0.038

1 

### VOC Trap 1 was extracted to give Exhaled VOC Trap 1 for all samples, and a second extract VOC Trap 1 Extract 2 for the samples collected

between 1 and 24 h.

2



### - Mean and SD not calculated for values less than limit of quantitation (<LOQ )RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### 31 Table 15

. Percentage of Radi oactivity as TBA in the DMF Extract s of VOC Trap 1 1 

### following Exposure to 1750 ppm [2

- 14 

### C]TBA/TBA

% of Radioactivity as TBA

Sample Timepoint 2

- 05 2

- 06 2

- 07 2

- 08 Mean SD

Exhaled VOC Trap 1 1 h 99. 6 70.8 82. 5 83.1 84.0 11.8

Exhaled VOC Trap 1 3 h 75. 0 72. 2 77. 5 79. 3 7 6.0 3. 1

Exhaled VOC Trap 1 5 h 79.6 66. 6 68. 5 75. 2 72. 5 6. 0

Exhaled VOC Trap 1 7 h 73 .8 70. 1 75. 2 71. 1 72.5 2. 4

Exhaled VOC Trap 1 12 h 69.7 68. 4 76.4 75.3 72. 5 4.0

Exhaled VOC Trap 1 24 h 42.6 40.5 56.4 57.4 49. 3 8. 9

Exhaled VOC Trap 1 48 h 26.8 42.9 6. 9 35. 9 28. 1 15.6

Exhaled VOC Trap 1 72 h 54.3 54.0 <LOQ 6 6.0 58.1 6.8

Exhaled VOC Trap 1 96 h <LOQ <LOQ <LOQ <LOQ

- 2



### -

Exhaled VOC Trap 1 120 h <LOQ <LOQ <LOQ <LOQ

- -

Exhaled VOC Trap 1 144 h NA 3 

### <LOQ <LOQ <LOQ

- -

Exhaled VOC Trap 1 168 h NA <LOQ NA 69. 9

- -

VOC Trap 1 Extract 2 1 h 92.1 96.2 91.3 89.2 92.2 2.9

VOC Trap 1 Extract 2 3 h 92.9 92.9 97.6 96.3 94.9 2.4

VOC Trap 1 Extract 2 5 h 84.0 85.3 93.5 94.1 89.2 5.3

VOC Trap 1 Extract 2 7 h 94.7 91.1 88.1 91.8 91.4 2.7

VOC Trap 1 Extract 2 12 h 91.9 92.8 92.8 92.1 92.4 0.5

VOC Trap 1 Extract 2 24 h 61.2 75.6 76.7 75.8 72.3 7. 4

1 

### VOC Trap 1 was extracted to give Exhaled VOC Trap 1 for all samples, and a second extract VOC Trap 1 Extract 2 for the samples

collected between 1 and 24 h

2



### - Mean and SD not calculated for values less than limit of quantitation (<LOQ)

. 3 

### NA = not an alyzed.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### 32 Table 16

. Analysis of Radioactivity in Urine following Exposure to 1750 ppm [2

- 14 

### C]TBA/TBA

Time point Animal Perce ntage of radioactivity present (% a 

### ) Recovery b 

### (%)

Peak 1 Peak 2 Peak 3 Peak 4 ( 14 

### C TBA) (HPLC)

2

- 05 35.7 36.1 19.6 4.13 108

12 hr 2

- 06 33.3 38.0 20.3 4.69 96.1

2

- 07 26.2 43.8 21.3 4.21 98.3

2

- 08 30.3 41.8 19.2 4.58 98.2

Mean 31.4 39.9 20.1 4.40

SD 4.10 3.51 0.920 0.274

CV% c 

### 13.1% 8.78% 4.58% 6.23%

2

- 05 71.0 11.5 14.3 0.469 95.7

24 hr 2

- 06 65.3 13.7 17.7 0.524 106

2

- 07 57.9 17.8 20.9 * 98.1

2

- 08 63.5 15.8 17.2 * 94.9

Mean 64.4 14.7 17.5 0.497

SD 5.40 2.71 2.70

-

CV% 8.38% 18.4% 15.4%

-

2

- 05 64.9 11.6 7.98 * 96.6

48 hr 2

- 06 71.9 10.4 12.5 * 96.4

2

- 07 81 .9 7.79 * * 97.4

2

- 08 76.2 8.82 * * 96.9

Mean 73.7 9.65 10.2

-

SD 7.17 1.68

- -

CV% 9.72% 17.5%

- -

a 

### Values represent (radioactivity detected in each peak/ total radioactivity recovered from the column)*100

b 

### Total recovery of radioacti vity in the collected eluent, as a percentage of the radioactivity injected.

c 

### CV% = coefficient of variation, calculated as (SD/Mean)*100.

* Peak not detected with radioactivity detector

.

- Values not calculated for less than three determinations

.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### 33 Figure 1 . Cumulative Excretion of Radioactivity in Urine and Feces.

Graph of cumulative recovery against time for Mean

Feces

Urine

0 1 2 3 4 5 6 7

Time (Days) 0 10 20 30 40 50 60 70 80Cumulative recovery (%)



### 0 1 2 3 4 5 6 7

Time (Days) 0 10 20 30 40 50 60 70 80Cumulative recovery (%)RTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### 34 Figure 2

. HPLC Analysis of Urinary Metabolites from Rat 2

- 06 Following Exposure to 1750 ppm [2

-

14 

### C]TBA/TBA

.

Sample from Rat 2

- 06 obtai ned at 12 h (top) 24 h (middle) and 48 h (bottom). Chromatograms

represent detection of radioactivity.

gmV

0.00 20.00 40.00 60.00 80.00 100.00 120.00 140.00 160.00

Minutes 0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00Peak1 - 3.820Peak2 - 5.077Peak3 - 7.750Peak4 (14C TBA) - 20.391

gmV

0.00 100.00 200.00 300.00 400.00

Minutes 0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00Peak1 - 3.870Peak2 - 5.092Peak3 - 7.667

gmV

0.00 20.00 40.00 60.00 80.00 100.00 120.00 140.00

Minutes 0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00Peak1 - 3.786Peak2 - 4.880Peak3 - 7.542RTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### 35 Figure 3 . HPLC Separation of 2

- M ethyl

- 1,2

- propanediol, α

- H ydroxyisobutyric A cid, and TBA.

Unlabeled standard 2

- me thyl

- 1,2

- propanediol and α

- hydroxyisobutyric acid were

chromatographed with labeled TBA to establish retention time in the HPLC system used for analysis of

urinary metabolites. The eluted peaks were detected with refractive index detection.mV

-200.00 -150.00 -100.00 -50.00

0.00 50.00 100.00 150.00 200.00

Minutes 0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.002-Methyl-1,2-propanediol - 8.0662-hydroxyisobutyric acid - 9.607[14C] TBA - 19.847RTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### 36 Figure 4 . HPLC Analysis of Urinary Metabolites from Rat 2

- 06 after Incubation with β

Glucuronidase or Acid Treatment

Sample from Rat 2

- 06 12 h urine following exposure to 1750 ppm [2

- 14 

### C]TBA/TBA, incubated with

buffer (top) , with β

- gl ucuronidase (middle) and with acid (bottom). Chromatograms represent detection of

radioactivity.

gmV

0.00 20.00 40.00 60.00 80.00 100.00 120.00

Minutes 0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00Peak1 - 3.775Peak2 - 5.141Peak3 - 7.832Peak4 (14C TBA) - 20.594

gmV

0.00 10.00 20.00 30.00 40.00 50.00 60.00 70.00

Minutes 0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00Peak1 - 3.851Peak3 - 7.718Peak4 (14C TBA) - 20.117

gmV

0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00

Minutes 0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.007.7838.73320.466RTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### A

- 1 Appendix A

Approved Study Protocol, Amendments, and DeviationsRTI Project No.: 0209 4 08 .004 Protocol No. RTI

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- 25 RTI Protocol No. RTI

- 935 (RTI Project No. 020908.004 )

Deviations from the Approved Protocol

The deviations listed below did not affect the results of the study.

Deviation

Protocol section 8.5.1 indicated that control samples of urine and feces will be collected prior to dosing on

study D ay 1 and analyzed for radiochemical content to determine background counts. The protocol

indicates that excreta are to be obtained from unexposed rats for a period of 48 hours (Section 3.0, Page

7).

Reason for the Deviation

Excreta were not collected prio r to exposure for determination of background because two unexposed

rats were used to generate background samples at the time of collection from the exposed animals.

Deviation

The stability of TBA was not monitored by sampling at the beginning of the exp osure, but it was sampled

at the end of the exposure by sampling at the top of the tower (inlet). The samples obtained (2) were

taken for GC

- MS and HPLC analysis.

Reason for the Deviation

This deviation was an oversight. The test chemical was sampled and purity and identity were checked in

the preparation used for exposure. A sample was collected at the end of the exposure to verify purity.

Deviation

The concentration of potassium hydroxide (KOH) was 1.0 N instead of 0.1 N which was stated in Section

8 .5.3 and 8.4.1 of the protocol.

Reason for the Deviation

The value of 0.1 N was a typographical error in the protocol. The concentration was intended to match

the concentration used in previous studies investigating TAME previously conducted by the sponso r

elsewhere. 1.0 N is the concentration used typically for trapping exhaled 14 

### C O 2 

### . The study protocol was

amended to include this change.

Deviation

The protocol indicates that aliquots of 0.1 N KOH will be analyzed for radioactivity by LSS after

neutrali zation, and addition of scintillation cocktail. Aliquots of the CO2 traps were analyzed without

neutralization

Reason for the Deviation

Neutralization of KOH trap aliquots was an error in the protocol. The protocol was amended.

Deviation

The protocol do es not specify that the animals were to be randomized and assigned into groups. The

animals were randomized according to SOP DPK

- HUS

- 001, and they were assigned to study groups

using the randomization.

Reason for the DeviationRTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### A

- 26 Section 7.2 indicated that animals would be specifically purchased for each study and would not be

randomly assigned to groups. However, both Study A and Study B were conducted on the same day, so

animals were randomly assigned to study A and B, and to unexposed animals.

Deviatio n

Charcoal in traps used to collect exhaled volatile organic compounds was removed from the traps and

subjected to 4 mL extractions with dimethylformamide.

Reason for the Deviation

The original procedure stated in the protocol was not effective for extra ction of volatile organic

compounds collected in the traps.

Deviation

The protocol indicated that " The chemical and radiochemical purity of the test substance will be verified

by RTI using GC methods based upon those available in the literature, or develo ped by RTI. "

Radiochemical purity was determined by high pressure liquid chromatography and not gas

chromatography.

Reason for the Deviation

The radiochemical purity of the radiolabeled material was determined by high pressure liquid

chromatography

. RTI does not have the capability to determine the radiochemical purity by gas

ch ro matography. This is an error in the development of the protocol.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### B

- 1 Appendix B

Test Chemical Analysis ReportRTI Project No.: 0209 4 08 .004 Protocol No. RTI

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- 1 Appendix C

Test Chemical Analysis Report: 2

- 14 

### C Tert iary Butyl AlcoholRTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### D

- 1 Appendix D

Reference Standards Analysis ReportRTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### E

- 1 Appendix E

Pre

- Study Inhalation ReportRTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### E

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- 17RTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### F

- 1 Appendix F

Inhalation ReportRTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### F

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### G

- 1 Appendix G

Example CalculationsRTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### G

- 2 Formulae for Calculations

F ormulae used in the Debra system for calculations are shown below:

Aliquot dpm/g is calculated by:

dpm/g = aliquot corrected count (dpm) / aliquot weight (g)

The mean of these aliquot figures for the sample is calculated by:

Mean dpm/g = Sum of all aliquot dpm/g / number of aliquots

The weight of the sample used for calculations is calculated from the pot weight (before sample

collection) and sample weight (weight of pot + sample) :

Weight of the sample = sample weight

– pot weight

Depending on the nature of the sample processing, Effective weight may be one of a number of values :

If processing type does not use homogenate (e.g. for urine, exhaled VOC trap, CO 2 

### trap, Cage Wash)

then:

Effective weight = weight of the sample

If processing type uses homogenate ( e.g. for feces and carcass) then:

Effective weight = Homogenate weight

To calculate the total dpm in the sample, Debra uses the previously calculated figures;

Total Dpm = Mean dpm/g * Effective weight

For inhalation exposure, where the dpm administered is not precisely known, the Dose D pm is calculated

as the sum of all dpm recovered for each animal.

Recovery is calculated by:

Recovery = 100* (Total Dpm / Dose D pm )

Concentration is calculated by:

Concentration = ( Total dpm/ effective weight) / (Specific acti vity)

Results are expressed in terms of the arithmetic mean ± standard deviation. Mean values were calculated

using:

Standard deviation was calculated using:RTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### G

- 3RTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### G

- 4 Example Calculations

Examples of the calculations for urine, feces, exhaled VOC trap, CO 2 

### tra p, and cage rinse made in the

DebraTM system are shown below:

Subject = 2

- 05

Tissue = Urine

Timepoint = 24 h

Aliquot 1 dpm/g = 1472068.215/0.5091

= 2891510.9310548 dpm/g

Aliquot 2 dpm/g = 1496832.465/0.5175

= 2892429.88405797 dpm/g

Sum of dpm/g = 578 3940.81511277 dpm/g

Number of aliquots = 2

Mean dpm/g = Sum of dpm/g / Number of aliquots

= 5783940.81511277 / 2

= 2891970.40755639 dpm/g

Recovery Effective Wt

= Sample weight

= 3.1866g

Concentration Effective Weight

= Sample weight

= 3.1866 g

Total dpm = Dpm/g x Recovery effective weight

= 2891970.40755639 x 3.1866

= 9215552.90071918 dpm

Dose dpm (using Exposure Dose)

= sum of all dpm recovered

= 49473192.2325294 dpm

Recovery = 100 x (Total dpm / Dose dpm)

= 100 x (9215552.9007 1918 / 49473192.2325294)

= 100 x (0.186273666300025)

= 18.6273666300025%

Concentration = (Total dpm/Concentration effective weight) / (Specific activity)

= (9215552.90071918 / 3.1866) / (0.2506030761 μCi/mg)

= (2891970.40755639 dpm/g) / (556338.82 8942 dpm/mg)

= 5.19821780740363 mg/g

Subject = 2

- 05

Tissue = Feces

Timepoint = 24 h

Aliquot 1 dpm/g = 3912.08625/0.1122

= 34867.0788770053 dpm/g

Aliquot 2 dpm/g = 3094.52625/0.0848

= 36492.0548349057 dpm/g

Sum of dpm/g = 71359.133711911 dpm/g

Num ber of aliquots = 2

Mean dpm/g = Sum of dpm/g / Number of aliquots

= 71359.133711911 / 2

= 35679.5668559555 dpm/g

Recovery Effective Weight

= Homogenate weight

= 12.8805g

Concentration Effective Weight

= Sample weightRTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### G

- 5 = 6.3064g

Total dpm = Dpm/g x Recovery effective weight

= 35679.5668559555 x 12.8805

= 459570.660888135 dpm

Dose dpm (using Exposure Dose)

= sum of all dpm recovered

= 49473192.2325294 dpm

Recovery = 100 x (Total dpm / Dose dpm)

= 100 x (459570.660888135 / 4947319 2.2325294)

= 100 x (0.00928928658430009)

= 0.928928658430009%

Concentration = (Total dpm/Concentration effective weight) / (Specific activity)

= (459570.660888135 / 6.3064) / (0.2506030761 μCi/mg)

= (72873.6935316718 dpm/g) / (556338.828942 dpm/mg )

= 0.130987969454257 mg/g

Subject = 2

- 05

Tissue = Carcass Digest

Timepoint = 168 h

Aliquot 1 dpm/g = 412.47/0.8479

= 486.460667531549 dpm/g

Aliquot 2 dpm/g = 451.32/0.9093

= 496.337842296272 dpm/g

Sum of dpm/g = 982.79850982782 dpm/g

Number of a liquots = 2

Mean dpm/g = Sum of dpm/g / Number of aliquots

= 982.79850982782 / 2

= 491.39925491391 dpm/g

Recovery Effective Weight

= Homogenate weight

= 583.38g

Concentration Effective Weight

= Sample weight

= 214.6g

Total dpm = Dpm/g x R ecovery effective weight

= 491.39925491391 x 583.38

= 286672.497331677 dpm

Dose dpm (using Exposure Dose)

= sum of all dpm recovered

= 49473192.2325294 dpm

Recovery = 100 x (Total dpm / Dose dpm)

= 100 x (286672.497331677 / 49473192.2325294)

= 100 x (0.00579450171689518)

= 0.579450171689518%

Concentration = (Total dpm/Concentration effective weight) / (Specific activity)

= (286672.497331677 / 214.6) / (0.2506030761 μCi/mg)

= (1335.84574711872 dpm/g) / (556338.828942 dpm/mg)

= 0.00240 11370007359 mg/g

Subject = 2

- 05

Tissue = CO2 Trap 1

Timepoint = 48 h

Aliquot 1 dpm/g = 52.13875/1.0448

= 49.9030915007657 dpm/g

Aliquot 2 dpm/g = 68.56875/1.0425

= 65.773381294964 dpm/gRTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### G

- 6 Sum of dpm/g = 115.67647279573 dpm/g

Number of aliquots = 2

M ean dpm/g = Sum of dpm/g / Number of aliquots

= 115.67647279573 / 2

= 57.8382363978649 dpm/g

Recovery Effective Wt

= Sample weight

= 529.19g

Concentration Effective Weight

= Sample weight

= 529.19g

Total dpm = Dpm/g x Recovery effective wei ght

= 57.8382363978649 x 529.19

= 30607.4163193861 dpm

Dose dpm (using Exposure Dose)

= sum of all dpm recovered

= 49473192.2325294 dpm

Recovery = 100 x (Total dpm / Dose dpm)

= 100 x (30607.4163193861 / 49473192.2325294)

= 100 x (0.00061866 6694793575)

= 0.0618666694793575%

Concentration = (Total dpm/Concentration effective weight) / (Specific activity)

= (30607.4163193861 / 529.19) / (0.2506030761 μCi/mg)

= (57.8382363978649 dpm/g) / (556338.828942 dpm/mg)

= 0.000103962249961695 mg/ g

Subject = 2

- 05

Tissue = Exhaled VOC Trap 1

Timepoint = 48 h

Aliquot 1 dpm/g = 2184.66857142857/0.0935

= 23365.4392666157 dpm/g

Aliquot 2 dpm/g = 2312.18857142857/0.0971

= 23812.4466676475 dpm/g

Sum of dpm/g = 47177.8859342632 dpm/g

Number of ali quots = 2

Mean dpm/g = Sum of dpm/g / Number of aliquots

= 47177.8859342632 / 2

= 23588.9429671316 dpm/g

Recovery Effective Wt

= Sample weight

= 3.8622g

Concentration Effective Weight

= Sample weight

= 3.8622g

Total dpm = Dpm/g x Recovery effective weight

= 23588.9429671316 x 3.8622

= 91105.2155276557 dpm

Dose dpm (using Exposure Dose)

= sum of all dpm recovered

= 49473192.2325294 dpm

Recovery = 100 x (Total dpm / Dose dpm)

= 100 x (91105.2155276557 / 49473192.2325294)

= 100 x (0.00184150671134079)

= 0.184150671134079%RTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### G

- 7 Concentration = (Total dpm/Concentration effective weight) / (Specific activity)

= (91105.2155276557 / 3.8622) / (0.2506030761 μCi/mg)

= (23588.9429671316 dpm/g) / (556338.828942 dpm/mg)

= 0.04240031746 83873 mg/g

Subject = 2

- 05

Tissue = Cage Rinse

Timepoint = 168 h

Aliquot 1 dpm/g = 295.995/0.892

= 331.832959641256 dpm/g

Aliquot 2 dpm/g = 316.605/0.8937

= 354.263175562269 dpm/g

Sum of dpm/g = 686.096135203525 dpm/g

Number of aliquots = 2

Mean dp m/g = Sum of dpm/g / Number of aliquots

= 686.096135203525 / 2

= 343.048067601762 dpm/g

Recovery Effective Wt

= Sample weight

= 200.08g

Concentration Effective Weight

= Sample weight

= 200.08g

Total dpm = Dpm/g x Recovery effective weight

= 343.048067601762 x 200.08

= 68637.0573657606 dpm

Dose dpm (using Exposure Dose)

= sum of all dpm recovered

= 49473192.2325294 dpm

Recovery = 100 x (Total dpm / Dose dpm)

= 100 x (68637.0573657606 / 49473192.2325294)

= 100 x (0.0013873585727 6318)

= 0.138735857276318%

Concentration = (Total dpm/Concentration effective weight) / (Specific activity)

= (68637.0573657606 / 200.08) / (0.2506030761 μCi/mg)

= (343.048067601762 dpm/g) / (556338.828942 dpm/mg)

= 0.00061661715802606 mg/g



### The c oncentrations of TBA determined in DMF extracts of Exhaled VOC Traps by gas chromatography

were calculated based on a calibration curve, with a linear regression (y=bx+a) of slope 1280, intercept

2.856, and linear correlation coefficient of 0.992. Concent rations were measured as μ g TBA/ μ l DMF.

Using the regression equation, concentrations for samples were calculated from peak areas:

Concentration = (Peak Area

– intercept)/Slope

C oncentrations measured as μ g TBA/ μ l DMF were converted to units of μ g TBA/mg DMF (or mg TBA/g

DMF) for comparison with values measured for radioactivity using the density of DMF for conversion, as

follows

Concentration ( μ g TBA/mg DMF) = Concentration ( μ g TBA / μ l DMF )/0.949RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 1 Appendix H

Raw Data TablesRTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 2 Sample Naming and Abbrevi ations

In the tables of raw data values in thi s appendix, the table headings are derived from the conventions of

sample naming used in the Debra  system. These are explained below. Example calculations and

formula e are provided in Appendix G.

Column Hea ding Source Explanation

Subject Animal number

Sample The type of sample, e.g. urine,

feces

Time The time of sample collection

Pot wt Balance The weight of the empty sample

container

Samp wt Balance Sample weight, the weight of

sample + empty sampl e container

Corr Samp Calculated The corrected sample weight,

calculated from Samp wt

– Pot wt

Homog wt Balance For samples requiring

homogenization, the weight of pot

+ sample + medium for

homogenization.

Corr Homog Calculated Corrected Ho mo genate We ight,

calculated from Homog Wt

– Samp

Wt.

Alq wt Balance Aliquot weight, weight of aliquot

removed for scintillation counting

Orig DPM Scintillation Counter Original DPM, the DPM measured

in each aliquot.

Bkg DPM Scintillation Counter The background DP M determined

for the batch of samples

Calc DPM Calculated The calculated DPM for the aliquot,

= Orig DPM

– Calc DPM

LOD Calculated Limit of Detection. If Orig D PM

value is less than Bkg D PM , then

the sample is flagged with an *RTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### H

- 3 Column Hea ding Source Explanation

LOQ Calculated Limit of Quantitation. If Orig D PM

value is less than 3 x Bkg DPM

,

then the sample is flagged with an

*

DPM/g Calculated Calculated value of DPM/g sample.

Sample DPM Calculated Total DPM in the sample.

Reco (%) Calculated Percentage of the total recovered

radio activity present in the sample.

Sample DPM Sum Calculated The sum of radioactivity in all

samples for a subject.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 4 Table 1. Preparation of 14 

### C TBA Formulation (Feedstock) for Inhalation Exposure

[ 14 

### C] TBA Test Material

Specific activity 7.3 m C i/mmol ( A )

Nonradiolabeled TBA Test Material

Molecular weight 74.12 g/mol or

mg/mmol ( B )

Density 0.781 mg/ μ L ( C )

Formulation (Feedstock)

Nonradiolabeled TBA 8.9693 g ( D

[14C] TBA 0.0309 g ( E )

Net Feedstock Weight 9.0002 g (F) =( D + E)

To tal TBA present in the formulation (by weight) 9.0002 g (F) =( D + E )

Total TBA present in the formulation (by weight) 9000.2 mg (G) =( D + E )*100

Nominal Specific Activity

Activity present 3.043308149 mCi (H) =(( E )*1000/( B ))*( A )

[14C]TBA and nonra diolabeled TBA 9000.2 mg (G)

Nominal Specific Activity 0.000338138 mCi/mg (I)=(H)/(G)

Nominal Specific Activity 0.338137836 μ Ci/mg (J)=(I)*1000

Nominal Specific Activity 25.06277638 μ Ci/mmol (K)=(((H)*1000)/(G))*(B)

Formulation Dilution

Weight of Feedstock 0.0121 g (L)

Weight of Water 10.1036 g (M)

Total weight of solution 10.1157 g (N)=(L)+(M)

Dose For mulation Dilution Aliquots

Aliq # Aliquot Wt

(g) DPM DPM/g

1 0.1186 78813.9 664535.4

2 0.1237 82635.8 668034

3 0.1288 85502.5 663839.3

Mean dilution DPM/g 665469.6 (O)

Total Radioactivity in the Dilution 6731690.334 DPM (P)

= (M)*(O )

Radiochemical Concentration of the Feedstock 556338044.1 DPM/g (Q)=(P)/(L)

Radiochemical Concentration of the Feedstock 250.6027226 μ C i/g (R)=(Q)/2220000

Specific Activity ( μ Ci/mg TBA) 0.250602723 μ C i/mg ( S)=(R)*(F)/(G)

Specific Activity ( μ Ci/mmol TBA) 18.5746738 μ C i/mmol (T) =(R)*(B)/1000RTI Project No.: 0209 4 08 .004 Protocol No. RTI

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### H

- 5 Table 2. Recovery of Radioactivity in Samples from Group 1.

Subject Sample Time Pot wt S amp wt Corr

Samp Homog

wt Corr

Homog Alq wt Orig DPM Bkg

DPM Calc DPM LOD LOQ DPM/g Sample DPM Reco very

(%)

1

- 01 Feces 0 h 17.1491 g 19.1633 g 2.0142 g 21.3519

g 4.2028 g 0.0775 g

0.0589 g 2355.72

18 13.94 19.27

19.27 2336.455

1794.675 30147.806

30469.864 127381.9731

dpm 0.215

1

- 01 Carcass Digest 0 h 107.92 g 293.96 g 186.04 g 783.61 g 675.69 g 0.4277 g

0.4578 g 37178.83

39886.08 4.03

4.03 37174.80 0

39882.050 86917.933

87116.754 58796748.8875

dpm 99.469

1

- 01 Exhaled VOC 0 h 15.1571 g 18.8776 g 3.7205 g N.A. N.A. 0.0905 g

0.0898 g 28.69

26.47 0.67

0.67 28.025

25.805 309.669

287.361 1110.623 8 dpm 0.002

1

- 01 Nose Only Tube

Rinse 0 h 81.9423 g 125.5502 g 43.6079

g N.A. N.A. 0.1507 g

0.1582 g 578.23

602.02 1.70

1.70 576.535

600.325 3825.713

3794.722 166155.5883

dpm 0.281

1

- 01 T ransfer Bag

Rinse 0 h 287.67 g 398.52 g 110.85 g N.A. N.A. 0.1642 g

0.1678 g 26.19

32.67 1.04

1.04 25.150

31.630 153.167

188.498 18936.7872 dpm 0.032

1

- 02 Feces 0 h 16.8063 g 17.2823 g 0.4760 g 18.4583

g 1.6520 g 0.0763 g

0.0668 g 1175.29

1021.04 19.27

19.27 1156.025

1001.775 15151.048

14996.632 24901.9839 dpm 0.041

1

- 02 Carcass Digest 0 h 107.28 g 300.18 g 192.90 g 769. 27 g 661.99 g 0.4304 g

0.4431 g 39197.21

40119.34 4.03

4.03 39193.180

40115.310 91062.221

90533.311 60107213.0993

dpm 99.623

1

- 02 Exhaled VOC 0 h 15.0588 g 18.9152 g 3.8564 g N.A. N.A. 0.0923 g

0.0 934 g 19.81

19.35 0.67

0.67 19.145

18.685 207.421

200.054 785.6933 dpm 0.001

1

- 02 Nose Only Tube

Rinse 0 h 81.6788 g 129.1637 g 47.4849

g N.A. N.A. 0.1634 g

0.1525 g 684.85

640.58 1.70

1.70 683.155

638.885 4180.875

4189.410 198731.0728

dpm 0.329

1

- 02 Transfer Bag

Rinse 0 h 288.36 g 528.87 g 240.51 g N.A. N.A. 0.1668 g

0.1601 g 1.45

4.79 1.04

1.04 0.410

3.750 *

* 2.458

23.423 3112.3069 dpm 0.005

1

- 03 Feces 0 h 17.2105 g 19.0064 g 1.7959 g 22.0264

g 4.8159 g 0.0420 g

0.0532 g 1321.20

1669.08 19.27

19.27 1301.935

1649.815 30998.452

31011.560 149317.0097

dpm 0.244

1

- 03 Carcass Digest 0 h 107.93 g 296.94 g 189.01 g 836.10 g 728.17 g 0.4344 g

0.4404 g 36033.02

36553.10 4.03

4.03 36028.990

36549.070 82939.664

82990.622 60412728.2023

dpm 98.765

1

- 03 Exhaled VOC 0 h 15.1591 g 19.0830 g 3.9239 g N.A. N.A. 0.0910 g

0.0907 g 62.28

53.18 0.67

0.67 61.615

52.515 677.088

578.997 2464.3752 dpm 0.004

1

- 03 Nose Only Tube

Rinse 0 h 80.3060 g 126.4599 g 46.1539

g N.A. N .A. 0.1481 g

0.1304 g 845.49

730.93 1.70

1.70 843.795

729.235 5697.468

5592.293 260533.2472

dpm 0.426

1

- 03 Transfer Bag

Rinse 0 h 286.08 g 443.75 g 157.67 g N.A. N.A. 0.1773 g

0.1554 g 382.59

343.23 1.04

1. 04 381.550

342.190 2152.002

2201.995 343247.3620

dpm 0.561

1

- 04 Feces 0 h 17.2253 g 20.6504 g 3.4251 g 23.7856

g 6.5603 g 0.1027 g

0.0603 g 13092.03

7614.69 19.27

19.27 13072.765

7595.425 127 290.798

125960.614 830702.6192

dpm 1.670

1

- 04 Carcass Digest 0 h 107.69 g 286.22 g 178.53 g 763.68 g 655.99 g 0.4276 g

0.4506 g 31375.46

33159.92 4.03

4.03 31371.430

33155.890 73366.300

73581.647 481981 91.8677

dpm 96.918

1

- 04 Exhaled VOC 0 h 15.2184 g 18.9558 g 3.7374 g N.A. N.A. 0.0899 g

0.0937 g 196.10

200.53 0.67

0.67 195.435

199.865 2173.915

2133.031 8048.3908 dpm 0.016

1

- 04 Nose Only Tube

Rinse 0 h 77.6745 g 123.5568 g 45.8823

g N.A. N.A. 0.1296 g

0.1284 g 1935.39

1876.51 1.70

1.70 1933.695

1874.815 14920.486

14601.363 677265.1671

dpm 1.362

1

- 04 Transfer Bag

Rinse 0 h 287.65 g 536.46 g 248.81 g N.A. N.A. 0.1486 g

0.1565 g 13.04

9.25 1.04

1.04 12.000

8.210 80.754

52.460 16572.4584 dpm 0.033



### * in LOD column indicates that sample DPM were less than background DPM, and in LOQ column indicates that sample DPM was less than 3 x background.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 6 Table 3 . Total Recovery of Radioactivity in Samples from Group 1.

Subject Sample Time Sample DPM Sample DPM Sum Reco very (%)

1

- 01 Feces 0 h 127381.9731 dpm 59110333.860 dpm 0.215

1

- 01 Carcass Digest 0 h 5 8796748.8875 dpm 99.469

1

- 01 Exhaled VOC 0 h 1110.6238 dpm 0.002

1

- 01 Nose Only Tube Rinse 0 h 166155.5883 dpm 0.281

1

- 01 Transfer Bag Rinse 0 h 18936.7872 dpm 0.032

1

- 02 Feces 0 h 24901.9839 dpm 60334744.156 dpm 0.041

1

- 02 Carcass Digest 0 h 6010 7213.0993 dpm 99.623

1

- 02 Exhaled VOC 0 h 785.6933 dpm 0.001

1

- 02 Nose Only Tube Rinse 0 h 198731.0728 dpm 0.329

1

- 02 Transfer Bag Rinse 0 h 3112.3069 dpm 0.005

1

- 03 Feces 0 h 149317.0097 dpm 61168290.196 dpm 0.244

1

- 03 Carcass Digest 0 h 60412728 .2023 dpm 98.765

1

- 03 Exhaled VOC 0 h 2464.3752 dpm 0.004

1

- 03 Nose Only Tube Rinse 0 h 260533.2472 dpm 0.426

1

- 03 Transfer Bag Rinse 0 h 343247.3620 dpm 0.561

1

- 04 Feces 0 h 830702.6192 dpm 49730780.503 dpm 1.670

1

- 04 Carcass Digest 0 h 48198191. 8677 dpm 96.918

1

- 04 Exhaled VOC 0 h 8048.3908 dpm 0.016

1

- 04 Nose Only Tube Rinse 0 h 677265.1671 dpm 1.362

1

- 04 Transfer Bag Rinse 0 h 16572.4584 dpm 0.033RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 7 Table 4. Recovery of Radioactivity in Urine from Group 2.

Subject Sample Time Po t wt Samp wt Corr Samp Alq wt Orig DPM Bkg DPM Calc DPM LOD LOQ DPM/g Sample DPM Reco (%)

2

- 05 Urine 12 h 48.4777 g 55.5796 g 7.1019 g 0.5018 g

0.5113 g 1637519.88

1661278.13 12.42

12.42 1637507.465

166 1265.715 3263267.168

3249101.731 23125096.3422 dpm 46.743

2

- 05 Urine 24 h 49.0173 g 52.2039 g 3.1866 g 0.5091 g

0.5175 g 1472080.63

1496844.88 12.42

12.42 1472068.215

1496832.465 2891510.931

2892429.884 9215552.9007 dpm 18.627

2

- 05 Urine 48 h 48.9178 g 57.6047 g 8.6869 g 0.5031 g

0.5104 g 191790.38

194940.59 12.42

12.42 191777.965

194928.175 381192.536

381912.569 3314508.8677 dpm 6. 700

2

- 05 Urine 72 h 48.3855 g 64.8394 g 16.4539 g 0.4994 g

0.4997 g 23677.51

21161.84 12.42

12.42 23665.095

21149.425 47387.054

42324.245 738050.3714 dpm 1.492

2

- 05 Urine 96 h 48.2028 g 57.1882 g 8.9854 g 0.5040 g

0.5043 g 7878.68

7930.01 12.42

12.42 7866.265

7917.595 15607.669

15700.169 140656.7202 dpm 0.284

2

- 05 Urine 120 h 48.3716 g 56.8512 g 8.4796 g 0.5059 g

0.5137 g 3846.39

3810.20 14.99

14.99 3831.400

3795.210 7573.433

7387.989 63433.4395 dpm 0.128

2

- 05 Urine 144 h 49.8184 g 58.5489 g 8.7305 g 0.5027 g

0.5109 g 2421.95

2505.26 14.99

14.99 2406.960

2490.270 4788.064

4874.281 42178.5521 dpm 0.085

2

- 05 Urine 168 h 48.2023 g 56.5844 g 8.3821 g 0.5082 g

0.5107 g 1601.91

1664.44 8.59

8.59 1593.320

1655.850 3135.2 22

3242.314 26728.5757 dpm 0.054

2

- 06 Urine 12 h 49.8359 g 55.9201 g 6.0842 g 0.5025 g

0.5088 g 1622134.25

1639983.00 12.42

12.42 1622121.835

1639970.585 3228103.154

3223212.628 19625547.7404 dpm 34.991

2

- 06 Urine 24 h 48.2608 g 50.4130 g 2.1522 g 0.4959 g

0.5164 g 2472429.75

2601387.25 12.42

12.42 2472417.335

2601374.835 4985717.554

5037519.045 10786004.9045 dpm 19.231

2

- 06 Urine 48 h 49.2520 g 57.9015 g 8.6495 g 0.5058 g

0.5120 g 398530.56

402483.47 12.42

12.42 398518.145

402471.055 787896.688

786076.279 6807039.5921 dpm 12.137

2

- 06 Urine 72 h 48.3592 g 60.0249 g 11.6657 g 0.5019 g

0.5042 g 64208.31

61098.18 12.42

12.42 64195.895

61085.765 127905.748

121153.838 1452727.2057 dpm 2.590

2

- 06 Urine 96 h 48.5784 g 57.9 105 g 9.3321 g 0.5000 g

0.5052 g 16424.76

16722.69 12.42

12.42 16412.345

16710.275 32824.690

33076.554 307498.4988 dpm 0.548

2

- 06 Urine 120 h 48.8772 g 58.1885 g 9.3113 g 0.5009 g

0.5066 g 8874. 07

8999.24 14.99

14.99 8859.080

8984.250 17686.325

17734.406 164906.5238 dpm 0.294

2

- 06 Urine 144 h 48.2892 g 57.1652 g 8.8760 g 0.4986 g

0.5010 g 12200.45

12256.26 14.99

14.99 12185.460

12241.270 24439.350

24433.673 216898.4753 dpm 0.387

2

- 06 Urine 168 h 49.6696 g 61.0100 g 11.3404 g 0.5019 g

0.5053 g 7996.96

8034.14 8.59

8.59 7988.370

8025.550 15916.258

15882.743 180306.6963 dpm 0.3 21

2

- 07 Urine 12 h 48.5781 g 53.3809 g 4.8028 g 0.5125 g

0.5121 g 1864389.63

1899674.88 12.42

12.42 1864377.215

1899662.465 3637809.200

3709553.730 17643957.3395 dpm 29.371

2

- 07 Urine 24 h 48.0274 g 51.5810 g 3.5536 g 0.5038 g

0.5159 g 2287319.75

2310785.50 12.42

12.42 2287307.335

2310773.085 4540109.835

4479110.457 16025350.6161 dpm 26.677

2

- 07 Urine 48 h 48.2920 g 58.3811 g 10.0891 g 0.5178 g

0.5093 g 328525.50

324468.25 12.42

12.42 328513.085

324455.835 634440.102

637062.311 6414157.4994 dpm 10.677

2

- 07 Urine 72 h 48.4478 g 59.1004 g 10.6526 g 0.5099 g

0.5128 g 61115.38

58673.90 12.42

12.42 61102.965

58661.485 119833.232

114394.472 1247567.0173 dpm 2.077

2

- 07 Urine 96 h 48.3720 g 58.1982 g 9.8262 g 0.5051 g

0.50 94 g 17897.08

18100.37 12.42

12.42 17884.665

18087.955 35408.167

35508.353 348419.9528 dpm 0.580

2

- 07 Urine 120 h 48.3381 g 58.3222 g 9.9841 g 0.5070 g

0.5124 g 7531.42

7617.89 14.99

14.99 7516.43 0

7602.900 14825.306

14837.822 148079.8168 dpm 0.247

2

- 07 Urine 144 h 48.6293 g 56.4096 g 7.7803 g 0.5056 g

0.5131 g 6264.36

6306.39 14.99

14.99 6249.370

6291.400 12360.305

12261.547 95782.697 7 dpm 0.159

2

- 07 Urine 168 h 48.5092 g 54.1272 g 5.6180 g 0.5096 g

0.5153 g 3792.09

3814.00 8.59

8.59 3783.500

3805.410 7424.451

7384.844 41599.3078 dpm 0.069RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 8 Table 4 (contd). Recovery of Ra dioactivity in Urine from Group 2.

Subject Sample Time Pot wt Samp wt Corr Samp Alq wt Orig DPM Bkg DPM Calc DPM LOD LOQ DPM/g Sample DPM Reco (%)

2

- 08 Urine 12 h 48.3010 g 54.7531 g 6.4521 g 0.5011 g

0.5119 g 1765224.25

1800520.50 12.42

12.42 1765211.835

1800508.085 3522673.788

3517304.327 22711321.3969 dpm 36.637

2

- 08 Urine 24 h 50.5195 g 54.1668 g 3.6473 g 0.5020 g

0.5119 g 1958559.38

19986 03.88 12.42

12.42 1958546.965

1998591.465 3901487.978

3904261.506 14234955.0469 dpm 22.963

2

- 08 Urine 48 h 50.3854 g 61.6526 g 11.2672 g 0.4996 g

0.5061 g 210063.56

213895.67 12.42

12.42 210051.1 45

213883.255 420438.641

422610.660 4749402.5413 dpm 7.662

2

- 08 Urine 72 h 50.5831 g 63.2441 g 12.6610 g 0.4988 g

0.5047 g 34314.61

34828.00 12.42

12.42 34302.195

34815.585 68769.437

68982.73 2 872040.1056 dpm 1.407

2

- 08 Urine 96 h 50.7012 g 61.7412 g 11.0400 g 0.4969 g

0.5048 g 13373.21

13565.48 12.42

12.42 13360.795

13553.065 26888.297

26848.385 296626.4898 dpm 0.479

2

- 08 Urine 120 h 50.3520 g 61.9769 g 11.6249 g 0.4969 g

0.5047 g 5473.85

5483.55 14.99

14.99 5458.860

5468.560 10985.832

10835.268 126834.0564 dpm 0.205

2

- 08 Urine 144 h 47.8162 g 54.3558 g 6.5396 g 0.4967 g

0.5065 g 3119.05

3174.78 14.99

14.99 3104.060

3159.790 6249.366

6238.480 40832.7575 dpm 0.066

2

- 08 Urine 168 h 48.0251 g 54.4967 g 6.4716 g 0.4 964 g

0.4989 g 2912.59

2923.72 8.59

8.59 2904.000

2915.130 5850.121

5843.115 37836.9722 dpm 0.061RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 9 Table 5 . Recovery of Radioactivity in Feces from Group 2.

Subject Sample Time Pot wt Samp wt Corr Samp Homog

wt Corr

Homog Alq wt Orig

DPM Bkg

D PM Calc DPM LOD LOQ DPM/g Sample DPM Reco (%)

2

- 05 Feces 0 h 17.3561 g 18.3092 g 0.9531 g 19.5465 g 2.1904 g 0.0793 g

0.1011 g 2266.02

2918.78 19.27

19.27 2246.755

2899.515 28332.346

28679.674 62 439.5633 dpm 0.126

2

- 05 Feces 24 h 50.4950 g 56.8014 g 6.3064 g 63.3755 g 12.8805 g 0.1122 g

0.0848 g 3930.69

3113.13 18.60

18.60 3912.086

3094.526 34867.079

36492.055 459570.6609

dpm 0.929

2

- 05 Feces 48 h 50.3150 g 56.4899 g 6.1749 g 63.5808 g 13.2658 g 0.0781 g

0.0719 g 408.38

379.13 18.60

18.60 389.776

360.526 4990.733

5014.273 66362.2065 dpm 0.134

2

- 05 Feces 72 h 48.1692 g 56.7115 g 8.5423 g 66.3532 g 18.1840 g 0.0726 g

0.0642 g 156.50

124.90 18.60

18.60 137.896

106.296 1899.397

1655.705 32322.9893 dpm 0.065

2

- 05 Feces 96 h 48.0192 g 54.7298 g 6.7106 g 60.2640 g 12.2448 g 0.0583 g

0.0743 g 52.03

76.80 18.60

18.60 33.426

58.196 *

* 573.349

783.260 8305.7059 dpm 0.017

2

- 05 Feces 120 h 50.2988 g 55.6046 g 5.30 58 g 60.6441 g 10.3453 g 0.0778 g

0.1007 g 34.16

45.39 18.60

18.60 15.556

26.786 *

* 199.952

266.000 2410.2081 dpm 0.005

2

- 05 Feces 144 h 48.3341 g 55.1847 g 6.8506 g 60.8989 g 12.5648 g 0.0788 g

0.0799 g 51.75

42.43 18.60

18.60 33.146

23.826 *

* 420.638

298.201 4516.0314 dpm 0.009

2

- 05 Feces 168 h 48.9074 g 57.3788 g 8.4714 g 67.9759 g 19.0685 g 0.0800 g

0.0582 g 34.75

29.87 18.60

18.6 0 16.146

11.266 *

* 201.828

193.578 3769.9026 dpm 0.008

2

- 06 Feces 0 h 17.3441 g 19.0451 g 1.7010 g 21.2880 g 3.9439 g 0.0858 g

0.0749 g 3228.24

2885.64 19.27

19.27 3208.975

2866.375 37400.6 41

38269.359 149217.4568

dpm 0.266

2

- 06 Feces 24 h 48.2585 g 53.0876 g 4.8291 g 60.3116 g 12.0531 g 0.0742 g

0.1289 g 3367.54

5616.20 18.60

18.60 3348.936

5597.596 45133.912

43425.882 533710.027 7

dpm 0.952

2

- 06 Feces 48 h 48.3841 g 54.1529 g 5.7688 g 60.4651 g 12.0810 g 0.1018 g

0.0844 g 1050.80

779.79 18.60

18.60 1032.196

761.186 10139.452

9018.794 115725.3897

dpm 0.206

2

- 06 Feces 72 h 50.3396 g 56.5580 g 6.2184 g 67.0466 g 16.7070 g 0.0913 g

0.0632 g 183.38

143.76 18.60

18.60 164.776

125.156 1804.778

1980.320 31618.8213 dpm 0.056

2

- 06 Feces 96 h 49.6338 g 56.1027 g 6.4689 g 62.1870 g 12.5532 g 0.0745 g

0.0714 g 230.57

216.53 18.60

18.60 211.966

197.926 2845.185

2772.076 35257.2997 dpm 0.063

2

- 06 Feces 120 h 50. 3651 g 56.6967 g 6.3316 g 64.9455 g 14.5804 g 0.0971 g

0.0901 g 44.40

45.00 18.60

18.60 25.796

26.396 *

* 265.667

292.966 4072.5462 dpm 0.007

2

- 06 Feces 144 h 49.9713 g 55.7063 g 5.7350 g 61.27 73 g 11.3060 g 0.0815 g

0.0628 g 71.02

60.68 18.60

18.60 52.416

42.076 643.144

670.004 7423.2265 dpm 0.013

2

- 06 Feces 168 h 50.2069 g 59.8452 g 9.6383 g 70.8147 g 20.6078 g 0.0523 g

0.0538 g 4 62.19

399.94 18.60

18.60 443.586

381.336 8481.573

7088.034 160427.6740

dpm 0.286

2

- 07 Feces 0 h 16.9432 g 17.9306 g 0.9874 g 19.5061 g 2.5629 g 0.0875 g

0.0804 g 1912.96

1709.77 19.27

19.27 189 3.695

1690.505 21642.229

21026.182 54677.4342 dpm 0.091

2

- 07 Feces 24 h 49.7696 g 57.4228 g 7.6532 g 64.6886 g 14.9190 g 0.0893 g

0.0924 g 3121.24

3092.33 18.60

18.60 3102.636

3073.726 3474 3.967

33265.436 507316.1385

dpm 0.845

2

- 07 Feces 48 h 50.0818 g 57.4051 g 7.3233 g 64.6862 g 14.6044 g 0.0844 g

0.0664 g 782.14

623.68 18.60

18.60 763.536

605.076 9046.638

9112.594 132602.3451

d pm 0.221

2

- 07 Feces 72 h 49.7447 g 55.7840 g 6.0393 g 64.0307 g 14.2860 g 0.0552 g

0.0752 g 124.64

156.25 18.60

18.60 106.036

137.646 1920.947

1830.402 26795.8846 dpm 0.045

2

- 07 Feces 96 h 50.3259 g 59.1257 g 8.7998 g 66.9389 g 16.6130 g 0.0886 g

0.0866 g 132.74

126.04 18.60

18.60 114.136

107.436 1288.220

1240.603 21005.6672 dpm 0.035

2

- 07 Feces 12 0 h 50.1903 g 58.6582 g 8.4679 g 68.3520 g 18.1617 g 0.0881 g

0.1038 g 61.66

56.44 18.60

18.60 43.056

37.836 488.720

364.511 7748.0653 dpm 0.013

2

- 07 Feces 144 h 49.7925 g 56.24 51 g 6.4526 g 61.8678 g 12.0753 g 0.0659 g

0.0882 g 44.45

51.73 18.60

18.60 25.846

33.126 *

* 392.204

375.581 4635.6181 dpm 0.008

2

- 07 Feces 168 h 49.8721 g 57.0401 g 7.1680 g 68.1028 g 18.2307 g 0.0790 g

0.0661 g 38.40

32.72 18.60

18.60 19.796

14.116 *

* 250.585

213.559 4230.8391 dpm 0.007



### * in LOD column indicates that sample DPM were less than background DPM, and in LOQ column indicates that sample DPM was less than 3 x background.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 10 T able 5 (contd). Recovery of Radioactivity in Feces from Group 2.

Subject Sample Time Pot wt Samp wt Corr Samp Homog

wt Corr

Homog Alq wt Orig

DPM Bkg

DPM Calc DPM LOD LOQ DPM/g Sample DPM Reco

(%)

2

- 08 Feces 0 h 17.0588 g 20.5817 g 3.5229 g 22.8737 g 5.8149 g 0.0717 g

0.0673 g 7982.10

7380.21 19.27

19.27 7962.835

7360.945 111057.671

109375.111 640897.2929

dpm 1.034

2

- 08 Feces 24 h 50.1918 g 56.3884 g 6.1966 g 63.7060 g 13.5142 g 0.1163 g

0.0825 g 4358.94

2992.43 18.60

18.60 4340.336

2973.826 37320.174

36046.379 495745.1346

dpm 0.800

2

- 08 Feces 48 h 49.7597 g 55.3926 g 5.6329 g 6 2.3769 g 12.6172 g 0.0817 g

0.0752 g 947.34

887.74 18.60

18.60 928.736

869.136 11367.641

11557.663 144626.5707

dpm 0.233

2

- 08 Feces 72 h 49.5965 g 56.8954 g 7.2989 g 65.3529 g 15.7564 g 0.0789 g

0.0590 g 303.33

219.56 18.60

18.60 284.726

200.956 3608.698

3406.038 55263.4920 dpm 0.089

2

- 08 Feces 96 h 50.7319 g 57.9331 g 7.2012 g 65.3341 g 14.6022 g 0.0976 g

0.0802 g 366.41

292.37 18. 60

18.60 347.806

273.766 3563.589

3413.544 50940.7450 dpm 0.082

2

- 08 Feces 120 h 49.9121 g 55.6105 g 5.6984 g 64.0965 g 14.1844 g 0.1097 g

0.0973 g 55.32

54.72 18.60

18.60 36.716

36.116 *

* 33 4.697

371.184 5006.2519 dpm 0.008

2

- 08 Feces 144 h 50.2497 g 64.5511 g 14.3014 g 72.7015 g 22.4518 g 0.0970 g

0.0640 g 698.30

495.70 18.60

18.60 679.696

477.096 7007.178

7454.629 162346.7963

dpm 0.262

2

- 08 Feces 168 h 50.2561 g 54.2132 g 3.9571 g 58.9658 g 8.7097 g 0.0770 g

0.0869 g 241.07

284.19 18.60

18.60 222.466

265.586 2889.172

3056.228 25891.3274 dpm 0.042



### * in LOD column ind icates that sample DPM were less than background DPM, and in LOQ column indicates that sample DPM was less than 3 x background.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 11 Table 6 . Recovery of Radioactivity in CO 2 

### Trap 1 from Group 2.

Subject Sample Time Pot wt Samp wt Corr Samp Alq wt Orig DP M Bkg DPM Calc DPM LOD LOQ DPM/g Sample DPM Reco (%)

2

- 05 CO2 Trap 1 1 h 0.00 g 504.89 g 504.89 g 1.0380 g

1.0373 g 71.16

64.19 15.65

15.65 55.509

48.539 53.477

46.793 25312.6590 dpm 0.051

2

- 05 CO2 Trap 1 3 h 0.00 g 567.25 g 567.25 g 1.0353 g

1.0381 g 72.03

68.70 15.65

15.65 56.379

53.049 54.456

51.102 29938.9474 dpm 0.061

2

- 05 CO2 Trap 1 5 h 0.00 g 486.09 g 486.09 g 1.0369 g

1.0352 g 72.99

80.69 15.65

15.65 57.339

65.039 55.298

62.827 28709.8064 dpm 0.058

2

- 05 CO2 Trap 1 7 h 0.00 g 383.70 g 383.70 g 1.0380 g

1.0362 g 70.06

73.01 15.65

15.65 54.409

57.359 52.417

55.355 20676.0218 dpm 0.042

2

- 05 CO2 Trap 1 12 h 0.00 g 487.96 g 487.96 g 1.0383 g

1.0372 g 98.92

106.80 15.65

15.65 83.269

91.149 80.197

87.880 41007.3838 dpm 0.083

2

- 05 CO2 Trap 1 24 h 0.00 g 520.19 g 520.19 g 1.0343 g

1.0346 g 170.80

181.44 15.65

15.65 155.149

165.789 150.004

160.244 80693.9335 dpm 0.163

2

- 05 CO2 Trap 1 48 h 0.00 g 529.19 g 529.19 g 1.0448 g

1.0425 g 67.79

84.22 15.65

15.65 52.139

68.569 49.903

65.773 30607.4163 dpm 0.062

2

- 05 CO2 Trap 1 72 h 0.00 g 574.29 g 574.29 g 1.0440 g

1.0452 g 30.11

41.34 15.65

15.65 14.459

25.689 *

* 13.849

24.578 11034.1810 dpm 0.022

2

- 05 CO2 Trap 1 96 h 0.00 g 504.54 g 504.54 g 1.0474 g

1.0451 g 32.48

26.80 16.48

16.48 16.002

10.322 *

* 15.278

9.877 6345.6999 dpm 0 .013

2

- 05 CO2 Trap 1 120 h 0.00 g 620.97 g 620.97 g 1.0540 g

1.0486 g 20.76

25.32 16.48

16.48 4.282

8.842 *

* 4.063

8.432 3879.4523 dpm 0.008

2

- 05 CO2 Trap 1 144 h 0.00 g 501.17 g 501.17 g 1.0445 g

1.0420 g 20.41

19.52 16.36

16.36 4.047

3.157 *

* 3.874

3.029 1729.9618 dpm 0.003

2

- 05 CO2 Trap 1 168 h 0.00 g 551.93 g 551.93 g 1.0472 g

1.0464 g 22.45

26.31 19.30

19.30 3.147

7.007 *

* 3.005

6.696 2677.0845 dpm 0.005

2

- 06 CO2 Trap 1 1 h 0.00 g 456.18 g 456.18 g 1.0363 g

1.0370 g 70.49

71.85 15.65

15.65 54.839

56.199 52.918

54.194 24431.0438 dpm 0.044

2

- 06 CO2 Trap 1 3 h 0.00 g 424.39 g 424.39 g 1.0375 g

1.0363 g 88.73

98.03 15.65

15.65 73.079

82.379 70.437

79.493 31814.5020 dpm 0.057

2

- 06 CO2 Trap 1 5 h 0.00 g 477.89 g 477.89 g 1.0375 g

1.0363 g 75.97

78.42 15.65

15.65 60.319

62.769 58.139

60.570 28364.8291 dpm 0.051

2

- 06 CO2 Trap 1 7 h 0.00 g 523.01 g 523.01 g 1.0369 g

1.0330 g 61.50

69.81 15.65

15.65 45.849

54.159 44.217

52.429 25273.3452 dpm 0.045

2

- 06 CO2 Trap 1 12 h 0.00 g 556.94 g 556.94 g 1.0352 g

1.0352 g 112.68

97.12 15.65

15.65 97.029

81.469 93.729

78.699 48016.0344 dpm 0 .086

2

- 06 CO2 Trap 1 24 h 0.00 g 444.82 g 444.82 g 1.0367 g

1.0339 g 194.94

203.67 15.65

15.65 179.289

188.019 172.942

181.854 78910.1092 dpm 0.141

2

- 06 CO2 Trap 1 48 h 0.00 g 603.68 g 603.68 g 1.0487 g

1.0459 g 84.60

80.93 15.65

15.65 68.949

65.279 65.747

62.414 38684.0640 dpm 0.069

2

- 06 CO2 Trap 1 72 h 0.00 g 483.60 g 483.60 g 1.0467 g

1.0439 g 45.46

42.09 15.65

15.65 29.809

26.439 *

* 28.479

25.327 13010.2157 dpm 0.023

2

- 06 CO2 Trap 1 96 h 0.00 g 529.65 g 529.65 g 1.0446 g

1.0467 g 28.46

27.72 16.48

16.48 11.982

11.242 *

* 11.470

10.740 588 1.9861 dpm 0.010

2

- 06 CO2 Trap 1 120 h 0.00 g 489.55 g 489.55 g 1.0438 g

1.0455 g 27.97

26.38 16.48

16.48 11.492

9.902 *

* 11.010

9.471 5013.1972 dpm 0.009

2

- 06 CO2 Trap 1 144 h 0.32 g 492.44 g 492.12 g 1.0417 g

1.0407 g 23.14

18.63 16.36

16.36 6.777

2.267 *

* 6.505

2.178 2136.6406 dpm 0.004

2

- 06 CO2 Trap 1 168 h 0.00 g 457.71 g 457.71 g 1.0441 g

1.0450 g 22.24

22.47 19.30

19.30 2.937

3.167 *

* 2.813

3.030 1337.1844 dpm 0.002



### * in LOD column indicates that sample DPM were less than background DPM, and in LOQ column indicates that sample DPM was less than 3 x background.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 12 Table 6 (contd). Recovery of Radioactivity in CO 2 

### Trap 1 from Group 2.

Subject Sample Time Pot wt Samp wt Corr Samp Alq wt Orig DPM Bkg DPM Calc DPM LOD LOQ DPM/g Sample DPM Reco (%)

2

- 07 CO2 Trap 1 1 h 0.00 g 476.12 g 476.12 g 1.0 407 g

1.0382 g 62.23

60.56 15.65

15.65 46.579

44.909 44.757

43.256 20952.4919 dpm 0.035

2

- 07 CO2 Trap 1 3 h 0.00 g 416.65 g 416.65 g 1.0365 g

1.0360 g 100.15

90.60 15.65

15.65 84.499

74.949 81.52 3

72.344 32054.4486 dpm 0.053

2

- 07 CO2 Trap 1 5 h 0.00 g 467.01 g 467.01 g 1.0370 g

1.0351 g 69.60

77.08 15.65

15.65 53.949

61.429 52.024

59.346 26005.3543 dpm 0.043

2

- 07 CO2 Tra p 1 7 h 0.00 g 421.22 g 421.22 g 1.0356 g

1.0337 g 84.77

85.65 15.65

15.65 69.119

69.999 66.743

67.717 28318.4957 dpm 0.047

2

- 07 CO2 Trap 1 12 h 0.01 g 420.44 g 420.43 g 1.0430 g

1.0364 g 150.88

171.80 15.65

15.65 135.229

156.149 129.654

150.665 58927.0911 dpm 0.098

2

- 07 CO2 Trap 1 24 h 0.00 g 486.98 g 486.98 g 1.0452 g

1.0419 g 291.66

268.28 15.65

15.65 276.009

252 .629 264.073

242.469 123337.9000 dpm 0.205

2

- 07 CO2 Trap 1 48 h 0.00 g 498.31 g 498.31 g 1.0480 g

1.0454 g 79.07

84.12 15.65

15.65 63.419

68.469 60.514

65.495 31395.8570 dpm 0.052

2

- 07 CO2 Trap 1 72 h 0.00 g 468.55 g 468.55 g 1.0466 g

1.0429 g 39.58

36.00 15.65

15.65 23.929

20.349 *

* 22.863

19.512 9927.4072 dpm 0.017

2

- 07 CO2 Trap 1 96 h 0.00 g 569.14 g 569.14 g 1.0477 g

1.0406 g 28.50

18.28 11.62

16.48 16.880

1.802 *

* 16.111

1.732 5077.6325 dpm 0.008

2

- 07 CO2 Trap 1 120 h 0.00 g 548.87 g 548.87 g 1.0413 g

1.0434 g 21.44

23.91 16.48

16.48 4. 962

7.432 *

* 4.765

7.123 3262.5011 dpm 0.005

2

- 07 CO2 Trap 1 144 h 0.01 g 458.50 g 458.49 g 1.0351 g

1.0384 g 16.14

22.24 16.36

16.36 0.000

5.877 *

* 0.000

5.659 1297.3772 dpm 0.002

2

- 07 CO2 Trap 1 168 h 0.00 g 550.13 g 550.13 g 1.0447 g

1.0452 g 24.04

22.02 19.30

19.30 4.737

2.717 *

* 4.534

2.599 1962.0883 dpm 0.003

2

- 08 CO2 Trap 1 1 h 0.00 g 425.31 g 425.31 g 1.0379 g

1.0315 g 67.81

65.34 15.65

15.65 52.159

49.689 50.254

48.171 20930.6686 dpm 0.034

2

- 08 CO2 Trap 1 3 h 0.00 g 435.55 g 435.55 g 1.0382 g

1.0351 g 85.62

91.79 15.65

15.65 69.969

76.139 67.394

73.557 30695.6456 dpm 0.050

2

- 08 CO2 Trap 1 5 h 0.00 g 419.30 g 419.30 g 1.0374 g

1.0358 g 74.10

90.35 15.65

15.65 58.449

74.699 56.342

72.117 26931.3325 dpm 0.043

2

- 08 CO2 Trap 1 7 h 0.00 g 506.98 g 506.98 g 1.0386 g

1.0339 g 67.77

69.81 15.65

15.65 52.119

54.159 50.182

52.383 25999.1264 dpm 0.042

2

- 08 CO2 Trap 1 12 h 0.00 g 49 8.68 g 498.68 g 1.0375 g

1.0421 g 132.60

130.13 15.65

15.65 116.949

114.479 112.722

109.854 55496.9970 dpm 0.090

2

- 08 CO2 Trap 1 24 h 0.00 g 426.07 g 426.07 g 1.0495 g

1.0441 g 321.69

294.40 15.65

1 5.65 306.039

278.749 291.604

266.975 118996.9798 dpm 0.192

2

- 08 CO2 Trap 1 48 h 0.00 g 443.14 g 443.14 g 1.0501 g

1.0464 g 105.05

93.49 15.65

15.65 89.399

77.839 85.134

74.387 35345.0111 dpm 0.05 7

2

- 08 CO2 Trap 1 72 h 0.00 g 473.10 g 473.10 g 1.0459 g

1.0352 g 18.62

29.24 15.65

15.65 2.969

13.589 *

* 2.838

13.127 3776.5574 dpm 0.006

2

- 08 CO2 Trap 1 96 h 0.00 g 457.10 g 457.10 g 1.0393 g

1.0373 g 34.75

29.58 16.48

16.48 18.272

13.102 *

* 17.581

12.631 6904.9372 dpm 0.011

2

- 08 CO2 Trap 1 120 h 0.00 g 480.46 g 480.46 g 1.0427 g

1.0412 g 23.24

25. 96 16.48

16.48 6.762

9.482 *

* 6.485

9.107 3745.6389 dpm 0.006

2

- 08 CO2 Trap 1 144 h 0.00 g 373.05 g 373.05 g 1.0407 g

1.0417 g 22.88

25.16 16.36

16.36 6.517

8.797 *

* 6.262

8.445 2743.1002 dpm 0.004

2

- 08 CO2 Trap 1 168 h 0.00 g 521.68 g 521.68 g 1.0356 g

1.0316 g 22.93

20.44 19.30

19.30 3.627

1.137 *

* 3.502

1.102 1200.8666 dpm 0.002



### * in LOD column indicates that sample DPM were less than backgr ound DPM, and in LOQ column indicates that sample DPM was less than 3 x background.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 13 Table 7 . Recovery of Radioactivity in CO 2 

### Trap 2 from Group 2.

Subject Sample Time Pot wt Samp wt Corr Samp Alq wt Orig DPM Bkg DPM Calc DPM LOD LOQ DPM/g Sample DP M Reco (%)

2

- 05 CO2 Trap 2 1 h 0.00 g 586.33 g 586.33 g 1.0367 g

1.0364 g 16.80

16.35 15.65

15.65 1.149

0.699 *

* 1.108

0.674 522.5057 dpm 0.001

2

- 05 CO2 Trap 2 3 h 0.00 g 447.27 g 447.27 g 1.0349 g

1.0348 g 15.00

17.71 15.65

15.65 0.000

2.059 *

* 0.000

1.990 444.9252 dpm 0.001

2

- 05 CO2 Trap 2 5 h 0.00 g 505.17 g 505.17 g 1.0359 g

1.0343 g 16.12

14.5 4 15.65

15.65 0.469

0.000 *

* 0.453

0.000 114.2960 dpm 0.000

2

- 05 CO2 Trap 2 7 h 0.00 g 362.03 g 362.03 g 1.0343 g

1.0319 g 12.07

17.05 15.65

15.65 0.000

1.399 *

* 0.000

1.356 245.3675 dpm 0.000

2

- 05 CO2 Trap 2 12 h 0.00 g 490.26 g 490.26 g 1.0317 g

1.0315 g 13.20

16.35 15.65

15.65 0.000

0.699 *

* 0.000

0.677 166.0539 dpm 0.000

2

- 05 CO2 Trap 2 24 h 0 .00 g 430.42 g 430.42 g 1.0251 g

1.0225 g 19.52

8.91 15.65

15.65 3.869

0.000 *

* 3.774

0.000 812.2073 dpm 0.002

2

- 05 CO2 Trap 2 48 h 0.00 g 439.74 g 439.74 g 1.0392 g

1.0386 g 25.84

25.39 15.65

15.65 10.189

9.739 *

* 9.804

9.377 4217.3753 dpm 0.009

2

- 05 CO2 Trap 2 72 h 0.00 g 429.90 g 429.90 g 1.0380 g

1.0388 g 20.21

17.93 15.65

11.62 4.559

6.310 *

* 4.392

6.074 2249.7045 dpm 0.005

2

- 05 CO2 Trap 2 96 h 0.00 g 515.61 g 515.61 g 0.6031 g

0.7338 g 7.42

12.60 16.48

16.48 0.000

0.000 *

* 0.000

0.000 0.0000 dpm 0.000

2

- 05 CO2 Trap 2 120 h 0.01 g 474.72 g 474.71 g 1.0404 g

1.0414 g 16.95

17.85 16.48

16.48 0.472

1.372 *

* 0.454

1.317 420.3863 dpm 0.001

2

- 05 CO2 Trap 2 144 h 0.00 g 488.66 g 488.66 g 1.0345 g

1.0358 g 14.11

20.20 16.36

16.36 0.000

3.837 *

* 0.000

3.704 905.0133 dpm 0.002

2

- 05 CO2 Trap 2 168 h 0.00 g 465.37 g 465.37 g 1.0371 g

1.0372 g 18.62

13.88 19.30

19.30 0.000

0.000 *

* 0.000

0.000 0.0000 dpm 0.000

2

- 06 CO2 T rap 2 1 h 0.00 g 539.72 g 539.72 g 1.0373 g

1.0358 g 15.90

15.00 15.65

15.65 0.249

0.000 *

* 0.240

0.000 64.7138 dpm 0.000

2

- 06 CO2 Trap 2 3 h 0.00 g 481.65 g 481.65 g 1 .0340 g

1.0330 g 17.93

18.61 15.65

15.65 2.279

2.959 *

* 2.204

2.864 1220.5133 dpm 0.002

2

- 06 CO2 Trap 2 5 h 0.00 g 445.61 g 445.61 g 1.0358 g

1.0341 g 13.20

18.18 15.65

15.65 0.000

2.529 *

* 0.000

2 .445 544.8391 dpm 0.001

2

- 06 CO2 Trap 2 7 h 0.00 g 495.89 g 495.89 g 1.0362 g

1.0318 g 22.22

12.08 15.65

15.65 6.569

0.000 *

* 6.339

0.000 1571.7899 dpm 0.003

2

- 06 CO2 Trap 2 12 h 0.00 g 457.67 g 457.67 g 1.0412 g

1.0379 g 11.40

20.19 15.65

15.65 0.000

4.539 *

* 0.000

4.373 1000.6984 dpm 0.002

2

- 06 CO2 Trap 2 24 h 0.00 g 469.49 g 469.49 g 1.0344 g

1 .0300 g 18.84

18.62 15.65

15.65 3.189

2.969 *

* 3.083

2.882 1400.2508 dpm 0.002

2

- 06 CO2 Trap 2 48 h 0.00 g 415.80 g 415.80 g 1.0400 g

1.0377 g 31.48

30.34 15.65

15.65 15.829

14.689 *

* 15.220

14.155 6107.0738 dpm 0.011

2

- 06 CO2 Trap 2 72 h 0.00 g 473.05 g 473.05 g 1.0390 g

1.0382 g 22.47

24.27 15.65

15.65 6.819

8.619 *

* 6.563

8.302 3515.8090 dpm 0.006

2

- 06 CO2 Trap 2 96 h 0.00 g 537.81 g 537.81 g 1.0375 g

1.0341 g 21.91

17.39 16.48

16.48 5.432

0.912 *

* 5.236

0.882 1645.0503 dpm 0.003

2

- 06 CO2 Trap 2 120 h 0.00 g 570.30 g 570.30 g 1.0361 g

1.035 8 g 16.26

16.71 16.48

16.48 0.000

0.232 *

* 0.000

0.224 63.8683 dpm 0.000

2

- 06 CO2 Trap 2 144 h 0.00 g 435.00 g 435.00 g 1.0372 g

1.0348 g 19.07

16.59 16.36

16.36 2.707

0.227 *

* 2.610

0.219 615.2279 d pm 0.001

2

- 06 CO2 Trap 2 168 h 0.00 g 503.78 g 503.78 g 1.0371 g

1.0414 g 18.19

21.11 19.30

19.30 0.000

1.807 *

* 0.000

1.735 436.9899 dpm 0.001



### * in LOD column indicates that sample DPM were less th an background DPM, and in LOQ column indicates that sample DPM was less than 3 x background.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 14 Table 7 (contd). Recovery of Radioactivity in CO 2 

### Trap 2 from Group 2.

Subject Sample Time Pot wt Samp wt Corr Samp Alq wt Orig DPM Bkg DPM Calc DPM LOD LOQ D PM/g Sample DPM Reco (%)

2

- 07 CO2 Trap 2 1 h 0.00 g 490.69 g 490.69 g 1.0364 g

1.0359 g 19.74

19.52 15.65

15.65 4.089

3.869 *

* 3.945

3.735 1884.2059 dpm 0.003

2

- 07 CO2 Trap 2 3 h 0.00 g 448.09 g 448.09 g 1.0371 g

1.0339 g 17.27

13.87 15.65

15.65 1.619

0.000 *

* 1.561

0.000 349.6990 dpm 0.001

2

- 07 CO2 Trap 2 5 h 0.00 g 446.02 g 446.02 g 1.0355 g

1.03 49 g 22.44

17.49 15.65

15.65 6.789

1.839 *

* 6.556

1.777 1858.2873 dpm 0.003

2

- 07 CO2 Trap 2 7 h 0.01 g 392.82 g 392.81 g 1.0367 g

1.0333 g 15.68

14.09 15.65

15.65 0.029

0.000 *

* 0.028

0.000 5.4467 dpm 0.000

2

- 07 CO2 Trap 2 12 h 0.00 g 482.78 g 482.78 g 1.0339 g

1.0326 g 12.30

15.68 15.65

15.65 0.000

0.029 *

* 0.000

0.028 6.7209 dpm 0.000

2

- 07 CO2 Trap 2 24 h 0.00 g 445.59 g 445.59 g 1.0368 g

1.0326 g 19.53

18.39 15.65

15.65 3.879

2.739 *

* 3.741

2.652 1424.4095 dpm 0.002

2

- 07 CO2 Trap 2 48 h 0.00 g 429.69 g 429.69 g 1.0422 g

1.0427 g 42.31

43. 01 15.65

15.65 26.659

27.359 *

* 25.579

26.238 11132.7684 dpm 0.019

2

- 07 CO2 Trap 2 72 h 0.00 g 459.73 g 459.73 g 1.0402 g

1.0393 g 25.17

23.61 15.65

15.65 9.519

7.959 *

* 9.151

7.658 3863.7279 dpm 0 .006

2

- 07 CO2 Trap 2 96 h 0.00 g 446.79 g 446.79 g 1.0356 g

1.0363 g 19.41

20.77 16.48

16.48 2.932

4.292 *

* 2.831

4.142 1557.7036 dpm 0.003

2

- 07 CO2 Trap 2 120 h 0.00 g 487.53 g 487.53 g 1.0364 g

1.0381 g 17.16

21.23 16.48

16.48 0.682

4.752 *

* 0.658

4.578 1276.2660 dpm 0.002

2

- 07 CO2 Trap 2 144 h 0.00 g 445.83 g 445.83 g 1.0269 g

1.0363 g 17.94

23.35 16.36

16.36 1.577

6.987 *

* 1.535

6.742 1845.1343 dpm 0.003

2

- 07 CO2 Trap 2 168 h 0.00 g 417.56 g 417.56 g 1.0445 g

1.0434 g 18.18

21.11 19.30

19.30 0.000

1.807 *

* 0.000

1.732 361.5065 dpm 0.001

2

- 08 CO2 Trap 2 1 h 0.00 g 532.29 g 532.29 g 1.0377 g

1.0347 g 13.65

12.06 15.65

15.65 0.000

0.000 *

* 0.000

0.000 0.0000 dpm 0.000

2

- 08 CO2 Trap 2 3 h 0.00 g 414.39 g 414.39 g 1.0341 g

1.0333 g 15.45

16.36 15.65

15.65 0.000

0.709 *

* 0.000

0.686 142.1170 dpm 0.000

2

- 08 CO2 Trap 2 5 h 0.00 g 460.63 g 460.63 g 1.0365 g

1.0336 g 19.74

18.84 15.65

15.65 4 .089

3.189 *

* 3.945

3.085 1619.0815 dpm 0.003

2

- 08 CO2 Trap 2 7 h 0.00 g 440.05 g 440.05 g 1.0366 g

1.0303 g 11.62

16.37 15.65

15.65 0.000

0.719 *

* 0.000

0.698 153.4922 dpm 0.000

2

- 08 CO2 Trap 2 12 h 0.00 g 465.49 g 465.49 g 1.0314 g

1.0288 g 13.65

14.78 15.65

15.65 0.000

0.000 *

* 0.000

0.000 0.0000 dpm 0.000

2

- 08 CO2 Trap 2 24 h 0.00 g 415.97 g 415.97 g 1.0388 g

1.0413 g 23.35

16.59 15.65

15.65 7.699

0.939 *

* 7.411

0.902 1728.9196 dpm 0.003

2

- 08 CO2 Trap 2 48 h 0.00 g 470.23 g 470.23 g 1.0409 g

1.0402 g 43.19

31.91 15.65

15.65 27.539

16.259 *

* 26.457

15.630 9895.3037 dpm 0.016

2

- 08 CO2 Trap 2 72 h 0.01 g 483.77 g 483.76 g 1.0368 g

1.0370 g 44.76

39.36 15.65

15.65 29.109

23.709 *

* 28.076

22.863 12320.9789 dpm 0.020

2

- 08 CO2 Trap 2 96 h 0.00 g 479.91 g 479.91 g 1.0323 g

1.0330 g 26.86

16.94 16.48

16.48 10.382

0.462 *

* 10.057

0.447 2520.5821 dpm 0.004

2

- 08 CO2 Trap 2 120 h 0.00 g 493.02 g 493.02 g 1.0325 g

1.0310 g 21.89

17.62 16.48

16.48 5.412

1.142 *

* 5.242

1.108 1565.1681 dpm 0.003

2

- 08 CO2 Trap 2 144 h 0.00 g 357.30 g 357.30 g 1.0382 g

1.0371 g 15.69

25.16 16.36

16.36 0.000

8.79 7 *

* 0.000

8.482 1515.3066 dpm 0.002

2

- 08 CO2 Trap 2 168 h 0.00 g 495.43 g 495.43 g 1.0350 g

1.0317 g 17.72

21.13 19.30

19.30 0.000

1.827 *

* 0.000

1.771 438.5894 dpm 0.001



### * in LOD column indicat es that sample DPM were less than background DPM, and in LOQ column indicates that sample DPM was less than 3 x background.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 15 Table 8 . Recovery of Radioactivity in Exhaled VOC Trap 1 from Group 2.

Subject Sample Time Pot wt Samp wt Corr Samp Alq wt Ori g DPM Bkg DPM Calc DPM LOD LOQ DPM/g Sample DPM Reco (%)

2

- 05 Exhaled VOC Trap 1 1 h 16.6587 g 19.0611 g 2.4024 g 0.0952 g

0.0915 g 42609.00

40409.26 0.56

0.56 42608.445

40408.705 447567.700

441625.191 1068098.5 005

dpm 2.159

2

- 05 Exhaled VOC Trap 1 3 h 5.5949 g 8.1508 g 2.5559 g 0.0962 g

0.0964 g 45589.39

45475.99 1.37

1.37 45588.017

45474.617 473887.907

471728.389 1208450.3452

dpm 2.443

2

- 05 Exha led VOC Trap 1 5 h 5.6200 g 8.3826 g 2.7626 g 0.0953 g

0.0949 g 46341.34

46268.63 1.37

1.37 46339.967

46267.257 486253.585

487536.951 1345096.8679

dpm 2.719

2

- 05 Exhaled VOC Trap 1 7 h 6.28 92 g 8.8687 g 2.5795 g 0.0944 g

0.0967 g 40170.29

40778.50 3.11

3.11 40167.180

40775.390 425499.788

421668.976 1092635.9138

dpm 2.209

2

- 05 Exhaled VOC Trap 1 12 h 6.3941 g 8.9155 g 2.5214 g 0.0949 g

0.0957 g 39733.92

39890.12 3.11

3.11 39730.810

39887.010 418659.747

416792.163 1053254.2231

dpm 2.129

2

- 05 Exhaled VOC Trap 1 24 h 6.3346 g 8.6143 g 2.2797 g 0.0942 g

0.0965 g 9206.38

9380.16 3.11

3.11 9203.270

9377.05 0 97699.257

97171.503 222123.4352 dpm 0.449

2

- 05 Exhaled VOC Trap 1 48 h 15.4945 g 19.3567 g 3.8622 g 0.0935 g

0.0971 g 2185.72

2313.24 1.05

1.05 2184.669

2312.189 23365.439

23812.447 91105.2155 dpm 0.184

2

- 05 Exhaled VOC Trap 1 72 h 15.3148 g 19.1512 g 3.8364 g 0.0937 g

0.0948 g 5.56

4.23 1.05

1.05 4.509

3.179 48.117

33.529 156.6140 dpm 0.000

2

- 05 Exhaled VOC Trap 1 96 h 15.4770 g 19.4526 g 3.9756 g 0.0942 g

0.0946 g 426.16

427.73 1.05

1.05 425.109

426.679 4512.830

4510.344 17936.2656 dpm 0.036

2

- 05 Exhaled VOC Trap 1 120 h 15.5003 g 19.2707 g 3.7704 g 0.0946 g

0.0946 g 465 .75

483.35 1.05

1.05 464.699

482.299 4912.247

5098.294 18871.8712 dpm 0.038

2

- 05 Exhaled VOC Trap 1 144 h 15.4025 g 19.2635 g 3.8610 g 0.0935 g

0.0920 g 233.14

215.81 1.05

1.05 232.089

214.759 2482.231

2334 .332 9298.3749 dpm 0.019

2

- 05 Exhaled VOC Trap 1 168 h 15.5671 g 19.5384 g 3.9713 g 0.0952 g

0.0981 g 214.17

217.05 1.05

1.05 213.119

215.999 2238.640

2201.820 8817.2009 dpm 0.018

2

- 06 Exha led VOC Trap 1 1 h 16.5047 g 18.8733 g 2.3686 g 0.0928 g

0.0950 g 59116.26

60404.46 0.56

0.56 59115.705

60403.905 637022.683

635830.579 1507440.1183

dpm 2.688

2

- 06 Exhaled VOC Trap 1 3 h 5. 4573 g 8.0748 g 2.6175 g 0.0962 g

0.0956 g 50487.18

50036.97 1.37

1.37 50485.807

50035.597 524800.485

523384.902 1371812.6259

dpm 2.446

2

- 06 Exhaled VOC Trap 1 5 h 5.4917 g 8.0427 g 2.5510 g 0.0968 g

0.0945 g 52829.12

51430.03 1.37

1.37 52827.747

51428.657 545741.185

544218.589 1390243.6913

dpm 2.479

2

- 06 Exhaled VOC Trap 1 7 h 6.2921 g 8.8802 g 2.5881 g 0.0943 g

0.0959 g 42269.10

42669.73 3.11

3.11 42265.990

42 666.620 448207.741

444907.404 1155735.6531

dpm 2.061

2

- 06 Exhaled VOC Trap 1 12 h 6.4080 g 9.0483 g 2.6403 g 0.0965 g

0.0975 g 61918.35

62157.82 3.11

3.11 61915.240

62154.710 641608.705

637484.205 1688594.5 049

dpm 3.011

2

- 06 Exhaled VOC Trap 1 24 h 6.3212 g 9.1878 g 2.8666 g 0.0981 g

0.0959 g 13209.38

12974.59 3.11

3.11 13206.270

12971.480 134620.489

135260.480 386820.3928 dpm 0.690

2

- 06 Exha led VOC Trap 1 48 h 15.5030 g 19.4476 g 3.9446 g 0.0939 g

0.0961 g 10079.20

10378.35 1.05

1.05 10078.149

10377.299 107328.526

107984.376 424661.6370 dpm 0.757

2

- 06 Exhaled VOC Trap 1 72 h 15. 3767 g 19.3583 g 3.9816 g 0.0948 g

0.0928 g 2070.28

2010.22 1.05

1.05 2069.229

2009.169 21827.306

21650.523 86555.6620 dpm 0.154

2

- 06 Exhaled VOC Trap 1 96 h 15.4565 g 19.1471 g 3.6906 g 0.0936 g

0.0947 g 6 55.83

667.72 1.05

1.05 654.779

666.669 6995.498

7039.795 25899.3251 dpm 0.046

2

- 06 Exhaled VOC Trap 1 120 h 15.4861 g 19.2296 g 3.7435 g 0.0949 g

0.0945 g 494.01

507.58 1.05

1.05 492.959

506.529 5194.505

53 60.091 19755.5654 dpm 0.035

2

- 06 Exhaled VOC Trap 1 144 h 15.4536 g 19.4112 g 3.9576 g 0.0937 g

0.0966 g 359.16

384.58 1.05

1.05 358.109

383.529 3821.863

3970.275 15419.0830 dpm 0.027

2

- 06 Exhaled VOC Trap 1 168 h 15.5544 g 19.2828 g 3.7284 g 0.0935 g

0.0938 g 381.27

376.35 1.05

1.05 380.219

375.299 4066.509

4001.051 15039.5447 dpm 0.027



### * in LOD column indicates that sample DPM were less than background DPM, and in LOQ column indicates that sample DPM was less than 3 x background.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 16 Table 8 (contd). Recovery of Radioactivity in Exhaled VOC Trap 1 from Group 2.

Subject Sample Time Pot wt Samp wt Corr Samp Alq wt Orig DPM Bkg DPM Calc DPM LOD LOQ DPM/g Sample DP M Reco (%)

2

- 07 Exhaled VOC Trap 1 1 h 16.4649 g 19.0175 g 2.5526 g 0.0963 g

0.0961 g 54791.73

54575.29 0.56

0.56 54791.175

54574.735 568963.396

567895.265 1450972.7090

dpm 2.415

2

- 07 Exhaled VOC Trap 1 3 h 5.5508 g 8.0311 g 2.4803 g 0.0965 g

0.0952 g 52033.66

51447.91 1.37

1.37 52032.287

51446.537 539194.680

540404.797 1338865.2919

dpm 2.229

2

- 07 Exhaled VOC Trap 1 5 h 5.6245 g 8.2297 g 2.6052 g 0.0962 g

0.0940 g 50508.80

49786.18 1.37

1.37 50507.427

49784.807 525025.225

529625.603 1373788.1686

dpm 2.287

2

- 07 Exhaled VOC Trap 1 7 h 6.2672 g 8.8190 g 2.5518 g 0.0958 g

0.0944 g 54 592.50

53545.45 3.11

3.11 54589.390

53542.340 569826.618

567185.805 1450714.1506

dpm 2.415

2

- 07 Exhaled VOC Trap 1 12 h 6.3533 g 8.9389 g 2.5856 g 0.0956 g

0.0944 g 82855.93

81814.11 3.11

3.11 82852.820

81811.0 00 866661.297

866641.949 2240814.4367

dpm 3.730

2

- 07 Exhaled VOC Trap 1 24 h 6.3045 g 9.4339 g 3.1294 g 0.0971 g

0.0956 g 23596.90

23228.77 3.11

3.11 23593.790

23225.660 242984.449

242946.234 760335.7402 dp m 1.266

2

- 07 Exhaled VOC Trap 1 48 h 15.3679 g 19.0664 g 3.6985 g 0.0948 g

0.0941 g 8604.37

8587.18 1.05

1.05 8603.319

8586.129 90752.306

91244.724 336558.0078 dpm 0.560

2

- 07 Exhaled VOC T rap 1 72 h 15.5164 g 19.2441 g 3.7277 g 0.0934 g

0.0960 g 601.52

589.66 1.05

1.05 600.469

588.609 6429.000

6131.339 23410.5878 dpm 0.039

2

- 07 Exhaled VOC Trap 1 96 h 15.3215 g 19.1944 g 3. 8729 g 0.0944 g

0.0960 g 435.31

455.73 1.05

1.05 434.259

454.679 4600.197

4736.235 18079.5335 dpm 0.030

2

- 07 Exhaled VOC Trap 1 120 h 15.3647 g 19.3883 g 4.0236 g 0.0971 g

0.0941 g 395.20

386.89 1.05

1.05 394. 149

385.839 4059.203

4100.304 16415.2946 dpm 0.027

2

- 07 Exhaled VOC Trap 1 144 h 15.4907 g 19.2182 g 3.7275 g 0.0936 g

0.0932 g 384.42

377.23 1.05

1.05 383.369

376.179 4095.818

4036.251 15156.1433 dpm 0.02 5

2

- 07 Exhaled VOC Trap 1 168 h 15.3582 g 19.2050 g 3.8468 g 0.0947 g

0.0956 g 263.70

283.70 1.05

1.05 262.649

282.649 2773.480

2956.575 11021.1881 dpm 0.018

2

- 08 Exhaled VOC Trap 1 1 h 16.1723 g 18.7103 g 2.5380 g 0.0960 g

0.0912 g 73812.57

70099.53 0.56

0.56 73812.015

70098.975 768875.156

768629.112 1951092.9162

dpm 3.147

2

- 08 Exhaled VOC Trap 1 3 h 5.5698 g 8.0776 g 2.507 8 g 0.0954 g

0.0967 g 60185.21

61414.21 1.37

1.37 60183.837

61412.837 630857.827

635086.212 1587367.2297

dpm 2.561

2

- 08 Exhaled VOC Trap 1 5 h 5.4883 g 8.1929 g 2.7046 g 0.0951 g

0.0956 g 56775.29

56915.98 1. 37

1.37 56773.917

56914.607 596991.763

595341.074 1612391.6954

dpm 2.601

2

- 08 Exhaled VOC Trap 1 7 h 6.2917 g 8.8823 g 2.5906 g 0.0969 g

0.0949 g 47799.45

47567.88 3.11

3.11 47796.340

47564.770 493254.283

501209.378 1288128.7802

dpm 2.078

2

- 08 Exhaled VOC Trap 1 12 h 6.3609 g 8.9430 g 2.5821 g 0.0967 g

0.0962 g 71343.36

70947.23 3.11

3.11 71340.250

70944.120 737748.190

737464.865 1904573.8148

dpm 3.072

2

- 08 Exhaled VOC Trap 1 24 h 6.3546 g 8.9252 g 2.5706 g 0.0952 g

0.0952 g 20182.22

20086.95 3.11

3.11 20179.110

20083.840 211965.441

210964.706 543592.1180 dpm 0.877

2

- 08 Exhaled VOC Trap 1 48 h 15.5288 g 19.2240 g 3.6952 g 0.0938 g

0.0944 g 3510.65

3513.97 1.05

1.05 3509.599

3512.919 37415.763

37213.120 137884.3251 dpm 0.222

2

- 08 Exhaled VOC Trap 1 72 h 15.4359 g 19.2739 g 3.8380 g 0.0949 g

0.0945 g 2259.23

2220.83 1.05

1.05 2258.179

2219.779 23795.348

23489.720 90740.0471 dpm 0.146

2

- 08 Exhaled VOC Trap 1 96 h 15.3461 g 19.3510 g 4.0049 g 0.0941 g

0.0936 g 793.48

814.51 1.05

1.05 792.4 29

813.459 8421.133

8690.797 34265.7827 dpm 0.055

2

- 08 Exhaled VOC Trap 1 120 h 15.3140 g 19.0620 g 3.7480 g 0.0935 g

0.0945 g 589.99

568.73 1.05

1.05 588.939

567.679 6298.808

6007.181 23061.4232 dpm 0.037

2

- 08 Exhaled VOC Trap 1 144 h 15.3891 g 19.2218 g 3.8327 g 0.0938 g

0.0955 g 441.41

451.52 1.05

1.05 440.359

450.469 4694.654

4716.948 18035.9248 dpm 0.029

2

- 08 Exhaled VOC Trap 1 168 h 15.4982 g 19.4540 g 3.9558 g 0.0935 g

0.0935 g 1000.56

975.18 1.05

1.05 999.509

974.129 10689.931

10418.487 41750.3412 dpm 0.067



### * in LOD column indicates that sample DPM were less than background DPM, and in LOQ column indicates that sample DPM was less than 3 x background.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 17 Table 9 . Recovery of Radioactivity in VOC Trap 1 Extract 2 from Group 2.

Subject Sample Time Pot wt Samp wt Corr Samp Alq wt Orig DPM Bkg DPM Calc DPM LOD LOQ DPM/g Sample DPM Reco (%)

2

- 05 VOC Trap 1 Extract 2 1 h 15.2429 g 20.5005 g 5.2576 g 0.0930 g

0.0935 g 16563.82

17160.06 0.00

0.00 16563.820

17160.060 178105.591

183530.053 950667.7832 dpm 1.922

2

- 05 VOC Trap 1 Extract 2 3 h 15.5891 g 20.7659 g 5.1768 g 0.0941 g

0.0947 g 18552.55

18597.69 0.15

0.15 18552.403

18597.543 197156.252

196383.773 1018639.0021 dpm 2.059

2

- 05 VOC Trap 1 Extract 2 5 h 15.3572 g 20.4054 g 5.0482 g 0.0 945 g

0.0960 g 21425.14

21882.97 0.15

0.15 21424.993

21882.823 226719.506

227946.076 1147621.3969 dpm 2.320

2

- 05 VOC Trap 1 Extract 2 7 h 15.3069 g 20.3657 g 5.0588 g 0.0937 g

0.0941 g 13415.99

13385.55 0.89

0.8 9 13415.100

13384.660 143170.758

142238.682 721914.6375 dpm 1.459

2

- 05 VOC Trap 1 Extract 2 12 h 15.5266 g 20.5913 g 5.0647 g 0.0935 g

0.0941 g 17599.39

17574.75 0.89

0.89 17598.500

17573.860 188219.251

1867 57.279 949571.8178 dpm 1.919

2

- 05 VOC Trap 1 Extract 2 24 h 15.4645 g 20.6807 g 5.2162 g 0.0939 g

0.0942 g 3319.49

3330.14 0.89

0.89 3318.600

3329.250 35341.853

35342.357 184351.4874 dpm 0.373

2

- 06 VOC Trap 1 Extract 2 1 h 15.0710 g 20.3594 g 5.2884 g 0.0933 g

0.0913 g 23194.21

22777.92 0.00

0.00 23194.210

22777.920 248598.178

249484.337 1317029. 7869 dpm 2.348

2

- 06 VOC Trap 1 Extract 2 3 h 15.4822 g 20.7871 g 5.3049 g 0.0940 g

0.0934 g 21261.65

21039.99 0.15

0.15 21261.503

21039.843 226186.206

225265.989 1197454.3726 dpm 2.135

2

- 06 VOC Trap 1 Extract 2 5 h 15.5796 g 20.6246 g 5.0450 g 0.0943 g

0.0929 g 22811.83

22526.41 0.15

0.15 22811.683

22526.263 241905.444

242478.615 1221858.7879 dpm 2.178

2

- 06 VOC Trap 1 Extract 2 7 h 15.6275 g 20.6480 g 5.0205 g 0.0947 g

0.0946 g 15575.44

15493.96 0.89

0.89 15574.550

15493.070 164461.985

163774.524 823955.6980 dpm 1.469

2

- 06 VOC Trap 1 Extract 2 12 h 15.5558 g 20.5583 g 5.0025 g 0.0946 g

0.0944 g 26917.67

26820.97 0.89

0.89 26916.780

26820.080 284532.558

284111.017 1422319.7422 dpm 2.536

2

- 06 VOC Trap 1 Extract 2 24 h 15.7434 g 20.4903 g 4.7469 g 0.0937 g

0.0941 g 5683.56

5728.98 0.89

0.89 5682.670

5728.090 60647.492

60872.370 288421.3160 dpm 0.514

2

- 07 VOC Trap 1 Extract 2 1 h 15.2044 g 20.3277 g 5.1233 g 0.0932 g

0.0957 g 26396.38

27060.66 0.00

0.00 26396.380

27060.660 283222.961

282765.517 1449864.3862 dpm 2.414

2

- 07 VOC Trap 1 Extract 2 3 h 15.4117 g 20.7273 g 5.3156 g 0.0947 g

0.0938 g 21999.11

21845.64 0.15

0.15 21998.963

21845.493 232301.619

232894.385 1236397.9404 dpm 2.058

2

- 07 VOC Trap 1 Extract 2 5 h 15.3420 g 20.6164 g 5.274 4 g 0.0942 g

0.0939 g 23272.73

23102.99 0.15

0.15 23272.583

23102.843 247055.025

246036.670 1300381.4180 dpm 2.165

2

- 07 VOC Trap 1 Extract 2 7 h 15.3742 g 20.5050 g 5.1308 g 0.0939 g

0.0958 g 19077.29

19512.60 0.89

0.89 19076.400

19511.710 203156.550

203671.294 1043676.1507 dpm 1.737

2

- 07 VOC Trap 1 Extract 2 12 h 15.4368 g 20.5857 g 5.1489 g 0.0943 g

0.0945 g 32103.48

32335.74 0.89

0.89 32102.590

32334.850 340430. 435

342167.725 1757314.8321 dpm 2.925

2

- 07 VOC Trap 1 Extract 2 24 h 15.3879 g 20.4294 g 5.0415 g 0.0944 g

0.0925 g 8933.41

8608.51 0.89

0.89 8932.520

8607.620 94624.153

93055.351 473093.1094 dpm 0.788

2

- 08 VOC Trap 1 Extract 2 1 h 14.8992 g 19.9990 g 5.0998 g 0.0925 g

0.0935 g 26759.04

27139.56 0.00

0.00 26759.040

27139.560 289286.919

290262.674 1477793.5065 dpm 2.384

2

- 08 VOC Trap 1 Extract 2 3 h 15.4964 g 20.5783 g 5.0819 g 0.0948 g

0.0929 g 28609.10

28134.04 0.15

0.15 28608.953

28133.893 301782.208

302840.617 1536316.3680 dpm 2.478

2

- 08 VOC Trap 1 Extract 2 5 h 15.5654 g 20.6280 g 5 .0626 g 0.0937 g

0.0945 g 26714.79

26889.67 0.15

0.15 26714.643

26889.523 285108.253

284545.220 1441963.8381 dpm 2.326

2

- 08 VOC Trap 1 Extract 2 7 h 15.3876 g 20.3979 g 5.0103 g 0.0948 g

0.0938 g 17776.98

17412 .43 0.89

0.89 17776.090

17411.540 187511.498

185624.094 934760.6276 dpm 1.508

2

- 08 VOC Trap 1 Extract 2 12 h 15.3812 g 20.4511 g 5.0699 g 0.0936 g

0.0936 g 24270.62

24348.38 0.89

0.89 24269.730

24347.490 2592 91.987

260122.756 1316690.4043 dpm 2.124

2

- 08 VOC Trap 1 Extract 2 24 h 15.4964 g 20.6431 g 5.1467 g 0.0948 g

0.0946 g 6953.84

6925.89 0.89

0.89 6952.950

6925.000 73343.354

73202.960 377114.9578 dpm 0.608



### * in LOD column indicates that sample DPM were less than background DPM, and in LOQ column indicates that sample DPM was less than 3 x background.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 18 Table 10 . Recovery of Radioactivity in Exhaled VOC Trap 2 from Group 2.

Subject Sample Time Pot wt Samp w t Corr Samp Alq wt Orig DPM Bkg DPM Calc DPM LOD LOQ DPM/g Sample DPM Reco (%)

2

- 05 Exhaled VOC Trap 2 1 h 15.3426 g 19.3568 g 4.0142 g 0.0933 g

0.0948 g 4.67

2.22 4.33

4.33 0.340

0.000 *

* 3.644

0.000 7.3142 dpm 0.000

2

- 05 Exhaled VOC Trap 2 3 h 15.4097 g 19.2040 g 3.7943 g 0.0936 g

0.0928 g 0.56

1.45 0.79

0.79 0.000

0.663 *

* 0.000

7.139 13.5438 dpm 0.000

2

- 05 Exhaled VOC Trap 2 5 h 15.4169 g 19.3380 g 3.9211 g 0.0937 g

0.0940 g 3.23

0.11 0.79

0.79 2.443

0.000 *

* 26.067

0.000 51.1061 dpm 0.000

2

- 05 Exhaled VOC Trap 2 7 h 15.2511 g 19.1544 g 3.9033 g 0.0933 g

0.0930 g 4.56

0.0 0 0.79

0.79 3.773

0.000 *

* 40.434

0.000 78.9132 dpm 0.000

2

- 05 Exhaled VOC Trap 2 12 h 15.4542 g 19.3548 g 3.9006 g 0.0958 g

0.0958 g 0.00

0.00 0.79

0.79 0.000

0.000 *

* 0.000

0.000 0.0000 dpm 0.000

2

- 05 Exhaled VOC Trap 2 24 h 15.6718 g 19.5417 g 3.8699 g 0.0943 g

0.0921 g 0.00

1.22 0.79

0.79 0.000

0.433 *

* 0.000

4.696 9.0865 dpm 0.000

2

- 05 Exhaled VOC Trap 2 48 h 15.4354 g 19.3379 g 3.9025 g 0.0941 g

0.0917 g 6.34

8.34 0.79

0.79 5.553

7.553 59.006

82.361 275.8430 dpm 0.001

2

- 05 Exhaled VOC Trap 2 72 h 15.3993 g 19.2778 g 3.8785 g 0.0941 g

0.0951 g 1367.72

1358.16 0.79

0.79 1366.933

1357.373 14526.382

14273.107 55849.4086 dpm 0.113

2

- 05 Exhaled VOC Trap 2 96 h 15.5076 g 19.3758 g 3.8682 g 0.0937 g

0.0943 g 1.67

0.33 0.79

0.79 0.883

0.000 *

* 9.418

0.000 18.2160 dpm 0.000

2

- 05 Exhaled VOC Trap 2 120 h 15.5718 g 19.5836 g 4.0118 g 0.0943 g

0.0946 g 0.00

0.00 0.79

0.79 0.000

0.000 *

* 0.000

0.000 0.0000 dpm 0.000

2

- 05 Exhaled VOC Trap 2 144 h 15.5 200 g 19.3984 g 3.8784 g 0.0942 g

0.0935 g 4.79

3.89 0.79

0.79 4.003

3.103 42.489

33.182 146.7416 dpm 0.000

2

- 05 Exhaled VOC Trap 2 168 h 15.5800 g 19.4838 g 3.9038 g 0.0942 g

0.0931 g 0.00

0.56 0.79

0.79 0 .000

0.000 *

* 0.000

0.000 0.0000 dpm 0.000

2

- 06 Exhaled VOC Trap 2 1 h 15.3245 g 19.3504 g 4.0259 g 0.0929 g

0.0955 g 0.00

1.56 4.33

4.33 0.000

0.000 *

* 0.000

0.000 0.0000 dpm 0.000

2

- 06 Exhaled VOC Trap 2 3 h 15.3156 g 19.0605 g 3.7449 g 0.0928 g

0.0931 g 6.34

0.00 0.79

0.79 5.553

0.000 *

* 59.833

0.000 112.0343 dpm 0.000

2

- 06 Exhaled VOC Trap 2 5 h 15.1946 g 19.1061 g 3.9115 g 0.0936 g

0.0922 g 4.34

0.56 0.79

0.79 3.553

0.000 *

* 37.954

0.000 74.2287 dpm 0.000

2

- 06 Exhaled VOC Trap 2 7 h 15.2829 g 19.1896 g 3.9067 g 0.0931 g

0.0952 g 1.89

0.11 0.79

0.79 1.103

0.000 *

* 11.842

0.000 23.1318 dpm 0.000

2

- 06 Exhaled VOC Trap 2 12 h 15.5104 g 19.4298 g 3.9194 g 0.0941 g

0.0933 g 3.67

0.00 0.79

0.79 2.883

0.000 *

* 30.632

0.000 60.0301 dpm 0.000

2

- 06 Exhaled VOC Trap 2 24 h 15.5566 g 19.4764 g 3.9198 g 0.0935 g

0.0907 g 0.78

4.78 0.79

0.79 0.000

3.993 *

* 0.000

44.019 86.2723 dpm 0.000

2

- 06 Exhaled VOC Trap 2 48 h 15.5687 g 19.5009 g 3.9322 g 0.0936 g

0.0943 g 11.90

9.90 0.79

0.79 11.113

9.113 118.723

96.633 423.4122 dpm 0.001

2

- 06 Exhaled VOC Trap 2 72 h 15.5236 g 19.4145 g 3.8909 g 0.0930 g

0.0948 g 14.35

6.56 0.79

0.79 13.563

5.773 145.833

60.891 402.1725 dpm 0.001

2

- 06 Exhaled VOC Trap 2 96 h 15.4612 g 19.4204 g 3.9592 g 0.0940 g

0.0939 g 17.02

16.35 0.79

0.79 16.233

15.563 172.686

165.735 669.9382 dpm 0.001

2

- 06 Exhaled VOC Trap 2 120 h 15.5035 g 19.4640 g 3.9605 g 0.0940 g

0.0933 g 11.68

14.34 0.79

0.79 10.893

13.553 115.878

145.257 517.1124 dpm 0.001

2

- 06 Exhaled VOC Trap 2 144 h 15.4627 g 19.3511 g 3.8 884 g 0.0942 g

0.0932 g 11.24

7.23 0.79

0.79 10.453

6.443 110.961

69.126 350.1237 dpm 0.001



### * in LOD column indicates that sample DPM were less than background DPM, and in LOQ column indicates that sample DPM was less than 3 x background.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 19 Table 10 (contd). Recovery of Radioactivity in Exhaled VOC Trap 2 from Group 2.

Subject Sample Time Pot wt Samp wt Corr Samp Alq wt Orig DPM Bkg DPM Calc DPM LOD LOQ DPM/g Sample DPM Reco (%)

2

- 06 Exhaled VOC Trap 2 168 h 15.4423 g 19.3899 g 3.9476 g 0.0936 g

0.0913 g 5.23

0.78 0.79

0.79 4.443

0.000 *

* 47.463

0.000 93.6817 dpm 0.000

2

- 07 Exhaled VOC Trap 2 1 h 15.0724 g 19.1245 g 4.0521 g 0.0935 g

0.0956 g 2.00

1.11 4.33

4. 33 0.000

0.000 *

* 0.000

0.000 0.0000 dpm 0.000

2

- 07 Exhaled VOC Trap 2 3 h 15.4542 g 19.4454 g 3.9912 g 0.0934 g

0.0935 g 4.34

8.78 0.79

0.79 3.553

7.993 38.035

85.481 246.4898 dpm 0.000

2

- 07 Exhaled VOC Trap 2 5 h 15.2801 g 19.1518 g 3.8717 g 0.0937 g

0.0959 g 0.11

5.23 0.79

0.79 0.000

4.443 *

* 0.000

46.324 89.6769 dpm 0.000

2

- 07 Exhaled VOC Trap 2 7 h 15.1926 g 19. 1153 g 3.9227 g 0.0928 g

0.0940 g 0.00

2.34 0.79

0.79 0.000

1.553 *

* 0.000

16.516 32.3936 dpm 0.000

2

- 07 Exhaled VOC Trap 2 12 h 15.5111 g 19.4129 g 3.9018 g 0.0938 g

0.0935 g 0.00

5.23 0.79

0.79 0.000

4.443 *

* 0.000

47.513 92.6938 dpm 0.000

2

- 07 Exhaled VOC Trap 2 24 h 15.4917 g 19.4203 g 3.9286 g 0.0943 g

0.0931 g 71.33

61.29 0.79

0.79 70.543

60.503 748.065

649.866 2745.9547 dpm 0.005

2

- 07 Exhaled VOC Trap 2 48 h 15.4092 g 19.3010 g 3.8918 g 0.0940 g

0.0940 g 0.00

5.00 0.79

0.79 0.000

4.213 *

* 0.000

44.814 87.2032 dpm 0.000

2

- 07 Exhaled VOC Trap 2 72 h 15.4296 g 19.3698 g 3.9402 g 0.0939 g

0.0946 g 0.00

4.79 0.79

0.79 0.000

4.003 *

* 0.000

42.310 83.3544 dpm 0.000

2

- 07 Exhaled VOC Trap 2 96 h 15.3534 g 19.2494 g 3.8960 g 0.0940 g

0.0947 g 0.00

1.00 0.79

0.79 0.000

0.213 *

* 0.000

2.244 4.3712 dpm 0.000

2

- 07 Exhaled VOC Trap 2 120 h 15.2014 g 18.9873 g 3.7859 g 0.0932 g

0.0962 g 0.00

0.00 0.79

0.79 0.000

0.000 *

* 0.000

0.000 0.0000 dpm 0.000

2

- 07 Exhaled VOC Trap 2 144 h 15.4584 g 19.3407 g 3.8823 g 0.0936 g

0.0941 g 2.56

5.23 0.79

0.79 1.773

4.443 18.937

47.210 128.4020 dpm 0.000

2

- 07 Exhaled VOC Trap 2 168 h 15.4710 g 19.3587 g 3.8877 g 0.0 941 g

0.0970 g 2.78

0.00 0.79

0.79 1.993

0.000 *

* 21.174

0.000 41.1596 dpm 0.000

2

- 08 Exhaled VOC Trap 2 1 h 15.3109 g 19.3126 g 4.0017 g 0.0934 g

0.0906 g 0.89

1.56 4.33

4.33 0.000

0.000 *

* 0.000

0.000 0. 0000 dpm 0.000

2

- 08 Exhaled VOC Trap 2 3 h 15.3969 g 19.3144 g 3.9175 g 0.0933 g

0.0942 g 1.00

3.00 0.79

0.79 0.213

2.213 *

* 2.278

23.487 50.4669 dpm 0.000

2

- 08 Exhaled VOC Trap 2 5 h 15.3218 g 19.1980 g 3.8762 g 0.0930 g

0.0947 g 1.67

1.45 0.79

0.79 0.883

0.663 *

* 9.489

6.996 31.9496 dpm 0.000

2

- 08 Exhaled VOC Trap 2 7 h 15.2221 g 19.1040 g 3.8819 g 0.0927 g

0.0913 g 0.00

1.67 0.79

0.79 0.000

0.883 *

* 0.000

9.666 18.7611 dpm 0.000

2

- 08 Exhaled VOC Trap 2 12 h 15.4036 g 19.3119 g 3.9083 g 0.0936 g

0.0938 g 0.00

0.00 0.79

0.79 0.000

0.000 *

* 0.000

0.000 0.0000 dpm 0.000

2

- 08 Exhaled VOC Trap 2 24 h 15.6988 g 19.6286 g 3.9298 g 0.0947 g

0.0945 g 2.78

4.34 0.79

0.79 1.993

3.553 21.040

37.593 115.2074 dpm 0.000

2

- 08 Exhaled VOC Trap 2 48 h 15.3274 g 19.2230 g 3.8956 g 0.0941 g

0.0970 g 4.34

1.22 0.79

0.79 3.553

0.433 *

* 37.752

4.459 82.2189 dpm 0.000

2

- 08 Exhaled VOC Trap 2 72 h 15.4340 g 19.4207 g 3.9867 g 0.0937 g

0.0935 g 4.78

1.44 0.79

0.79 3.993

0.653 *

* 42.609

6.979 98.8462 dpm 0.000

2

- 08 Exhaled VOC Trap 2 96 h 15.4458 g 19.3784 g 3.9326 g 0.0934 g

0.0936 g 7.67

9.90 0.79

0.79 6.883

9.113 73.688

97.356 336.3242 dpm 0.001

2

- 08 Exhaled VOC Trap 2 120 h 15.4225 g 19.3224 g 3.8999 g 0.0935 g

0.0961 g 11.23

14.13 0.79

0.79 10.443

13.343 111.684

138.840 488.5097 dpm 0.001

2

- 08 Exhaled VOC Trap 2 144 h 15.4 391 g 19.3232 g 3.8841 g 0.0938 g

0.0928 g 5.00

8.12 0.79

0.79 4.213

7.333 44.909

79.014 240.6654 dpm 0.000

2

- 08 Exhaled VOC Trap 2 168 h 15.2489 g 19.1267 g 3.8778 g 0.0939 g

0.0937 g 1.00

1.67 0.79

0.79 0 .213

0.883 *

* 2.263

9.418 22.6491 dpm 0.000



### * in LOD column indicates that sample DPM were less than background DPM, and in LOQ column indicates that sample DPM was less than 3 x background.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 20 Table 11. Recovery of Radioactivity in Nose Only Tube R inse from Group 2.

Subject Sample Time Pot wt Samp wt Corr Samp Alq wt Orig DPM Bkg DPM Calc DPM LOD LOQ DPM/g Sample DPM Reco (%)

2

- 05 Nose Only Tube

Rinse 0 h 83.8656 g 134.9639 g 51.0983 g 0.1686 g

0.1655 g 11 22.26

1138.91 1.70

1.70 1120.565

1137.215 6646.293

6871.390 345365.3037 dpm 0.698

2

- 06 Nose Only Tube

Rinse 0 h 79.5942 g 129.8091 g 50.2149 g 0.1474 g

0.1488 g 405.76

415.32 1.70

1.70 404.065

413.625 2741. 282

2779.738 138618.7368 dpm 0.247

2

- 07 Nose Only Tube

Rinse 0 h 84.2557 g 127.9618 g 43.7061 g 0.1409 g

0.1264 g 455.13

419.69 1.70

1.70 453.435

417.995 3218.133

3306.922 142592.3728 dpm 0.237

2

- 08 Nose Only Tube

Rinse 0 h 78.3760 g 125.9693 g 47.5933 g 0.1332 g

0.1302 g 331.08

302.35 1.70

1.70 329.385

300.655 2472.860

2309.178 113796.4976 dpm 0.184



### Table 12 . Recovery of Radioactivity in Transfer Bag Rinse fro m Group 2.

Subject Sample Time Pot wt Samp wt Corr Samp Alq wt Orig DPM Bkg DPM Calc DPM LOD LOQ DPM/g Sample DPM Reco (%)

2

- 05 Transfer Bag Rinse 0 h 290.66 g 496.78 g 206.12 g 0.1516 g

0.1404 g 8.80

5.90 1.0 4

1.04 7.760

4.860 51.187

34.615 8842.8283 dpm 0.018

2

- 06 Transfer Bag Rinse 0 h 287.30 g 583.63 g 296.33 g 0.1317 g

0.1336 g 8.35

9.91 1.04

1.04 7.310

8.870 55.505

66.392 18060.8914 dpm 0.032

2

- 07 Transfer Bag Rinse 0 h 288.07 g 603.49 g 315.42 g 0.1212 g

0.1244 g 1.22

5.23 1.04

1.04 0.180

4.190 *

* 1.485

33.682 5546.1593 dpm 0.009

2

- 08 Transfer Bag Rinse 0 h 287.34 g 580.22 g 292.88 g 0.1193 g

0.1251 g 3.67

5.46 1.04

1.04 2.630

4.420 22.045

35.332 8402.2877 dpm 0.014



### * in LOD column indicates that sample DPM were less than background DPM, and in LOQ column indicates that sample DPM was less than 3 x backgr ound.



### Table 13 . Recovery of Radioactivity in Cage Rinse from Group 2.

Subject Sample Time Pot wt Samp wt Corr Samp Alq wt Orig DPM Bkg DPM Calc DPM LOD LOQ DPM/g Sample DPM Reco (%)

2

- 05 Cage Rinse 168 h 169.96 g 370.04 g 200.08 g 0.8920 g

0.8937 g 298.38

318.99 2.39

2.39 295.995

316.605 331.833

354.263 68637.0574 dpm 0.139

2

- 06 Cage Rinse 168 h 168.90 g 358.74 g 189.84 g 0.8893 g

0.9284 g 1075.24

1128.62 2.39

2.39 1072.855

1126.235 1206.404

1213.092 229658.5917 dpm 0.409

2

- 07 Cage Rinse 168 h 169.17 g 353.03 g 183.86 g 0.9166 g

0.9270 g 793.43

795.41 2.39

2.39 791.045

793.025 863.021

855.47 5 157981.3002 dpm 0.263

2

- 08 Cage Rinse 168 h 169.62 g 386.55 g 216.93 g 0.9011 g

0.9239 g 890.18

939.75 2.39

2.39 887.795

937.365 985.235

1014.574 216909.2616 dpm 0.350RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 21 Table 14 . Recovery of R adioactivity in Carcass Digest from Group 2.

Subject Sample Time Pot wt Samp

wt Corr

Samp Homog

wt Corr

Homog Alq wt Orig

DPM Bkg

DPM Calc

DPM LOD LOQ DPM/g Sample DPM Reco

(%)

2

- 05 Carcass Digest 168 h 107.75 g 322.35 g 214.60 g 691.13 g 583.38 g 0.8479 g

0.9093 g 413.43

452.28 0.96

0.96 412.470

451.320 486.461

496.338 286672.4973

dpm 0.579

2

- 06 Carcass Digest 168 h 107.74 g 324.03 g 216.29 g 724.91 g 617. 17 g 0.8404 g

0.8884 g 617.09

615.51 0.96

0.96 616.130

614.550 733.139

691.749 439699.1232

dpm 0.784

2

- 07 Carcass Digest 168 h 107.66 g 327.39 g 219.73 g 677.63 g 569.97 g 0.8519 g

0.9302 g 567.26

601.2 6 0.96

0.96 566.300

600.300 664.749

645.345 373357.2728

dpm 0.622

2

- 08 Carcass Digest 168 h 107.91 g 314.63 g 206.72 g 859.98 g 752.07 g 0.8656 g

0.9196 g 375.01

377.41 0.96

0.96 374.050

376.450 432. 128

409.363 316429.9817

dpm 0.510RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 22 Table 15 . Total Recovery of Radioactivity in Samples from Group 2

.

Subject Sample Time Sample DPM Sample DPM Sum Reco very

(%)

2

- 05 Urine 12 h 23125096.3422 dpm 49473192.233 dpm 46.743

2

- 05 Urine 24 h 9215552.90 07 dpm 18.627

2

- 05 Urine 48 h 3314508.8677 dpm 6.700

2

- 05 Urine 72 h 738050.3714 dpm 1.492

2

- 05 Urine 96 h 140656.7202 dpm 0.284

2

- 05 Urine 120 h 63433.4395 dpm 0.128

2

- 05 Urine 144 h 42178.5521 dpm 0.085

2

- 05 Urine 168 h 26728.5757 dpm 0.054

2

- 05 Feces 0 h 62439.5633 dpm 0.126

2

- 05 Feces 24 h 459570.6609 dpm 0.929

2

- 05 Feces 48 h 66362.2065 dpm 0.134

2

- 05 Feces 72 h 32322.9893 dpm 0.065

2

- 05 Feces 96 h 8305.7059 dpm 0.017

2

- 05 Feces 120 h 2410.2081 dpm 0.005

2

- 05 Feces 144 h 4516. 0314 dpm 0.009

2

- 05 Feces 168 h 3769.9026 dpm 0.008

2

- 05 Carcass Digest 168 h 286672.4973 dpm 0.579

2

- 05 CO2 Trap 1 1 h 25312.6590 dpm 0.051

2

- 05 CO2 Trap 1 3 h 29938.9474 dpm 0.061

2

- 05 CO2 Trap 1 5 h 28709.8064 dpm 0.058

2

- 05 CO2 Trap 1 7 h 2 0676.0218 dpm 0.042

2

- 05 CO2 Trap 1 12 h 41007.3838 dpm 0.083

2

- 05 CO2 Trap 1 24 h 80693.9335 dpm 0.163

2

- 05 CO2 Trap 1 48 h 30607.4163 dpm 0.062

2

- 05 CO2 Trap 1 72 h 11034.1810 dpm 0.022

2

- 05 CO2 Trap 1 96 h 6345.6999 dpm 0.013

2

- 05 CO2 Trap 1 120 h 3879.4523 dpm 0.008

2

- 05 CO2 Trap 1 144 h 1729.9618 dpm 0.003

2

- 05 CO2 Trap 1 168 h 2677.0845 dpm 0.005

2

- 05 CO2 Trap 2 1 h 522.5057 dpm 0.001

2

- 05 CO2 Trap 2 3 h 444.9252 dpm 0.001

2

- 05 CO2 Trap 2 5 h 114.2960 dpm 0.000

2

- 05 CO2 Trap 2 7 h 245.3675 dpm 0.000

2

- 05 CO2 Trap 2 12 h 166.0539 dpm 0.000

2

- 05 CO2 Trap 2 24 h 812.2073 dpm 0.002

2

- 05 CO2 Trap 2 48 h 4217.3753 dpm 0.009

2

- 05 CO2 Trap 2 72 h 2249.7045 dpm 0.005

2

- 05 CO2 Trap 2 96 h 0.0000 dpm 0.000

2

- 05 CO2 Trap 2 120 h 420.3863 dpm 0.001

2

- 05 CO2 Trap 2 144 h 905.0133 dpm 0.002

2

- 05 CO2 Trap 2 168 h 0.0000 dpm 0.000

2

- 05 Exhaled VOC Trap 1 1 h 1068098.5005 dpm 2.159

2

- 05 Exhaled VOC Trap 1 3 h 1208450.3452 dpm 2.443

2

- 05 Exhaled VOC Trap 1 5 h 1345096.8679 dpm 2.719

2

- 05 Exhaled VOC Trap 1 7 h 1092635.9138 dpm 2.209

2

- 05 Exhaled VOC Trap 1 12 h 1053254.2231 dpm 2.129

2

- 05 Exhaled VOC Trap 1 24 h 222123.4352 dpm 0.449

2

- 05 Exhaled VOC Trap 1 48 h 91105.2155 dpm 0.184

2

- 05 Exhaled VOC Trap 1 72 h 156.61 40 dpm 0.000

2

- 05 Exhaled VOC Trap 1 96 h 17936.2656 dpm 0.036

2

- 05 Exhaled VOC Trap 1 120 h 18871.8712 dpm 0.038

2

- 05 Exhaled VOC Trap 1 144 h 9298.3749 dpm 0.019

2

- 05 Exhaled VOC Trap 1 168 h 8817.2009 dpm 0.018

2

- 05 Exhaled VOC Trap 2 1 h 7.31 42 dpm 0.000

2

- 05 Exhaled VOC Trap 2 3 h 13.5438 dpm 0.000

2

- 05 Exhaled VOC Trap 2 5 h 51.1061 dpm 0.000

2

- 05 Exhaled VOC Trap 2 7 h 78.9132 dpm 0.000

2

- 05 Exhaled VOC Trap 2 12 h 0.0000 dpm 0.000

2

- 05 Exhaled VOC Trap 2 24 h 9.0865 dpm 0.000

2

- 05 Exhaled VOC Trap 2 48 h 275.8430 dpm 0.001

2

- 05 Exhaled VOC Trap 2 72 h 55849.4086 dpm 0.113

2

- 05 Exhaled VOC Trap 2 96 h 18.2160 dpm 0.000

2

- 05 Exhaled VOC Trap 2 120 h 0.0000 dpm 0.000

2

- 05 Exhaled VOC Trap 2 144 h 146.7416 dpm 0.000

2

- 05 E xhaled VOC Trap 2 168 h 0.0000 dpm 0.000RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 23 Table 15 (contd). Total Recovery of Radioactivity in Samples from Group 2.

Subject Sample Time Sample DPM Sample DPM Sum Reco very

(%)

2

- 05 Cage Rinse 168 h 68637.0574 dpm 0.139

2

- 05 Nose Only Tube Rinse 0 h 345365.3037 dpm 0.698

2

- 05 Transfer Bag Rinse 0 h 8842.8283 dpm 0.018

2

- 05 VOC Trap 1 Extract 2 1 h 950667.7832 dpm 1.922

2

- 05 VOC Trap 1 Extract 2 3 h 1018639.0021 dpm 2.059

2

- 05 VOC Trap 1 Extract 2 5 h 1147621.3969 dpm 2.320

2

- 05 VOC Trap 1 Ex tract 2 7 h 721914.6375 dpm 1.459

2

- 05 VOC Trap 1 Extract 2 12 h 949571.8178 dpm 1.919

2

- 05 VOC Trap 1 Extract 2 24 h 184351.4874 dpm 0.373

2

- 06 Urine 12 h 19625547.7404 dpm 56087309.043 dpm 34.991

2

- 06 Urine 24 h 10786004.9045 dpm 19.231

2

- 06 Uri ne 48 h 6807039.5921 dpm 12.137

2

- 06 Urine 72 h 1452727.2057 dpm 2.590

2

- 06 Urine 96 h 307498.4988 dpm 0.548

2

- 06 Urine 120 h 164906.5238 dpm 0.294

2

- 06 Urine 144 h 216898.4753 dpm 0.387

2

- 06 Urine 168 h 180306.6963 dpm 0.321

2

- 06 Feces 0 h 149 217.4568 dpm 0.266

2

- 06 Feces 24 h 533710.0277 dpm 0.952

2

- 06 Feces 48 h 115725.3897 dpm 0.206

2

- 06 Feces 72 h 31618.8213 dpm 0.056

2

- 06 Feces 96 h 35257.2997 dpm 0.063

2

- 06 Feces 120 h 4072.5462 dpm 0.007

2

- 06 Feces 144 h 7423.2265 dpm 0.013

2

- 06 Feces 168 h 160427.6740 dpm 0.286

2

- 06 Carcass Digest 168 h 439699.1232 dpm 0.784

2

- 06 CO2 Trap 1 1 h 24431.0438 dpm 0.044

2

- 06 CO2 Trap 1 3 h 31814.5020 dpm 0.057

2

- 06 CO2 Trap 1 5 h 28364.8291 dpm 0.051

2

- 06 CO2 Trap 1 7 h 25273.3452 dpm 0.045

2

- 06 CO2 Trap 1 12 h 48016.0344 dpm 0.086

2

- 06 CO2 Trap 1 24 h 78910.1092 dpm 0.141

2

- 06 CO2 Trap 1 48 h 38684.0640 dpm 0.069

2

- 06 CO2 Trap 1 72 h 13010.2157 dpm 0.023

2

- 06 CO2 Trap 1 96 h 5881.9861 dpm 0.010

2

- 06 CO2 Trap 1 120 h 5013.19 72 dpm 0.009

2

- 06 CO2 Trap 1 144 h 2136.6406 dpm 0.004

2

- 06 CO2 Trap 1 168 h 1337.1844 dpm 0.002

2

- 06 CO2 Trap 2 1 h 64.7138 dpm 0.000

2

- 06 CO2 Trap 2 3 h 1220.5133 dpm 0.002

2

- 06 CO2 Trap 2 5 h 544.8391 dpm 0.001

2

- 06 CO2 Trap 2 7 h 1571.7899 dpm 0.003

2

- 06 CO2 Trap 2 12 h 1000.6984 dpm 0.002

2

- 06 CO2 Trap 2 24 h 1400.2508 dpm 0.002

2

- 06 CO2 Trap 2 48 h 6107.0738 dpm 0.011

2

- 06 CO2 Trap 2 72 h 3515.8090 dpm 0.006

2

- 06 CO2 Trap 2 96 h 1645.0503 dpm 0.003

2

- 06 CO2 Trap 2 120 h 63.8683 dpm 0.000

2

- 06 CO2 Trap 2 144 h 615.2279 dpm 0.001

2

- 06 CO2 Trap 2 168 h 436.9899 dpm 0.001

2

- 06 Exhaled VOC Trap 1 1 h 1507440.1183 dpm 2.688

2

- 06 Exhaled VOC Trap 1 3 h 1371812.6259 dpm 2.446

2

- 06 Exhaled VOC Trap 1 5 h 1390243.6913 dpm 2.479

2

- 06 Exhaled VOC Trap 1 7 h 1155735.6531 dpm 2.061

2

- 06 Exhaled VOC Trap 1 12 h 1688594.5049 dpm 3.011

2

- 06 Exhaled VOC Trap 1 24 h 386820.3928 dpm 0.690

2

- 06 Exhaled VOC Trap 1 48 h 424661.6370 dpm 0.757

2

- 06 Exhaled VOC Trap 1 72 h 86555.6620 d pm 0.154

2

- 06 Exhaled VOC Trap 1 96 h 25899.3251 dpm 0.046

2

- 06 Exhaled VOC Trap 1 120 h 19755.5654 dpm 0.035

2

- 06 Exhaled VOC Trap 1 144 h 15419.0830 dpm 0.027

2

- 06 Exhaled VOC Trap 1 168 h 15039.5447 dpm 0.027

2

- 06 Exhaled VOC Trap 2 1 h 0.0000 dpm 0.000

2

- 06 Exhaled VOC Trap 2 3 h 112.0343 dpm 0.000RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 24 Table 15 (contd). Total Recovery of Radioactivity in Samples from Group 2.

Subject Sample Time Sample DPM Sample DPM Sum Reco very

(%)

2

- 06 Exhaled VOC Trap 2 5 h 74.2287 dpm 0.000

2

- 06 Exhaled VOC Trap 2 7 h 23.1318 dpm 0.000

2

- 06 Exhaled VOC Trap 2 12 h 60.0301 dpm 0.000

2

- 06 Exhaled VOC Trap 2 24 h 86.2723 dpm 0.000

2

- 06 Exhaled VOC Trap 2 48 h 423.4122 dpm 0.001

2

- 06 Exhaled VOC Trap 2 72 h 402.1725 dpm 0.001

2

- 06 Exhaled VO C Trap 2 96 h 669.9382 dpm 0.001

2

- 06 Exhaled VOC Trap 2 120 h 517.1124 dpm 0.001

2

- 06 Exhaled VOC Trap 2 144 h 350.1237 dpm 0.001

2

- 06 Exhaled VOC Trap 2 168 h 93.6817 dpm 0.000

2

- 06 Cage Rinse 168 h 229658.5917 dpm 0.409

2

- 06 Nose Only Tube Rin se 0 h 138618.7368 dpm 0.247

2

- 06 Transfer Bag Rinse 0 h 18060.8914 dpm 0.032

2

- 06 VOC Trap 1 Extract 2 1 h 1317029.7869 dpm 2.348

2

- 06 VOC Trap 1 Extract 2 3 h 1197454.3726 dpm 2.135

2

- 06 VOC Trap 1 Extract 2 5 h 1221858.7879 dpm 2.178

2

- 06 VOC Trap 1 Extract 2 7 h 823955.6980 dpm 1.469

2

- 06 VOC Trap 1 Extract 2 12 h 1422319.7422 dpm 2.536

2

- 06 VOC Trap 1 Extract 2 24 h 288421.3160 dpm 0.514

2

- 07 Urine 12 h 17643957.3395 dpm 60071898.654 dpm 29.371

2

- 07 Urine 24 h 16025350.6161 dpm 26.677

2

- 07 Urine 48 h 6414157.4994 dpm 10.677

2

- 07 Urine 72 h 1247567.0173 dpm 2.077

2

- 07 Urine 96 h 348419.9528 dpm 0.580

2

- 07 Urine 120 h 148079.8168 dpm 0.247

2

- 07 Urine 144 h 95782.6977 dpm 0.159

2

- 07 Urine 168 h 41599.3078 dpm 0.069

2

- 07 Feces 0 h 54677.4342 dpm 0.091

2

- 07 Feces 24 h 507316.1385 dpm 0.845

2

- 07 Feces 48 h 132602.3451 dpm 0.221

2

- 07 Feces 72 h 26795.8846 dpm 0.045

2

- 07 Feces 96 h 21005.6672 dpm 0.035

2

- 07 Feces 120 h 7748.0653 dpm 0.013

2

- 07 Feces 144 h 4635.6181 dpm 0.008

2

- 07 Feces 168 h 4230.8391 dpm 0.007

2

- 07 Carcass Digest 168 h 373357.2728 dpm 0.622

2

- 07 CO2 Trap 1 1 h 20952.4919 dpm 0.035

2

- 07 CO2 Trap 1 3 h 32054.4486 dpm 0.053

2

- 07 CO2 Trap 1 5 h 26005.3543 dpm 0.043

2

- 07 CO2 Trap 1 7 h 28318.4957 dpm 0.047

2

- 07 CO2 Trap 1 12 h 58927.0911 dpm 0.098

2

- 07 CO2 Trap 1 24 h 123337.9000 dpm 0.205

2

- 07 CO2 Trap 1 48 h 31395.8570 dpm 0.052

2

- 07 CO2 Trap 1 72 h 9927.4072 dpm 0.017

2

- 07 CO2 Trap 1 96 h 5077.6325 dpm 0.008

2

- 07 CO2 Trap 1 120 h 32 62.5011 dpm 0.005

2

- 07 CO2 Trap 1 144 h 1297.3772 dpm 0.002

2

- 07 CO2 Trap 1 168 h 1962.0883 dpm 0.003

2

- 07 CO2 Trap 2 1 h 1884.2059 dpm 0.003

2

- 07 CO2 Trap 2 3 h 349.6990 dpm 0.001

2

- 07 CO2 Trap 2 5 h 1858.2873 dpm 0.003

2

- 07 CO2 Trap 2 7 h 5.4 467 dpm 0.000

2

- 07 CO2 Trap 2 12 h 6.7209 dpm 0.000

2

- 07 CO2 Trap 2 24 h 1424.4095 dpm 0.002

2

- 07 CO2 Trap 2 48 h 11132.7684 dpm 0.019

2

- 07 CO2 Trap 2 72 h 3863.7279 dpm 0.006

2

- 07 CO2 Trap 2 96 h 1557.7036 dpm 0.003

2

- 07 CO2 Trap 2 120 h 1276. 2660 dpm 0.002

2

- 07 CO2 Trap 2 144 h 1845.1343 dpm 0.003

2

- 07 CO2 Trap 2 168 h 361.5065 dpm 0.001RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 25 Table 15 (contd). Total Recovery of Radioactivity in Samples from Group 2.

Subject Sample Time Sample DPM Sample DPM Sum Reco very

(%)

2

- 07 Exhale d VOC Trap 1 1 h 1450972.7090 dpm 2.415

2

- 07 Exhaled VOC Trap 1 3 h 1338865.2919 dpm 2.229

2

- 07 Exhaled VOC Trap 1 5 h 1373788.1686 dpm 2.287

2

- 07 Exhaled VOC Trap 1 7 h 1450714.1506 dpm 2.415

2

- 07 Exhaled VOC Trap 1 12 h 2240814.4367 dpm 3.730

2

- 07 Exhaled VOC Trap 1 24 h 760335.7402 dpm 1.266

2

- 07 Exhaled VOC Trap 1 48 h 336558.0078 dpm 0.560

2

- 07 Exhaled VOC Trap 1 72 h 23410.5878 dpm 0.039

2

- 07 Exhaled VOC Trap 1 96 h 18079.5335 dpm 0.030

2

- 07 Exhaled VOC Trap 1 120 h 16415.2946 dpm 0 .027

2

- 07 Exhaled VOC Trap 1 144 h 15156.1433 dpm 0.025

2

- 07 Exhaled VOC Trap 1 168 h 11021.1881 dpm 0.018

2

- 07 Exhaled VOC Trap 2 1 h 0.0000 dpm 0.000

2

- 07 Exhaled VOC Trap 2 3 h 246.4898 dpm 0.000

2

- 07 Exhaled VOC Trap 2 5 h 89.6769 dpm 0.000

2

- 07 Exhaled VOC Trap 2 7 h 32.3936 dpm 0.000

2

- 07 Exhaled VOC Trap 2 12 h 92.6938 dpm 0.000

2

- 07 Exhaled VOC Trap 2 24 h 2745.9547 dpm 0.005

2

- 07 Exhaled VOC Trap 2 48 h 87.2032 dpm 0.000

2

- 07 Exhaled VOC Trap 2 72 h 83.3544 dpm 0.000

2

- 07 Exhal ed VOC Trap 2 96 h 4.3712 dpm 0.000

2

- 07 Exhaled VOC Trap 2 120 h 0.0000 dpm 0.000

2

- 07 Exhaled VOC Trap 2 144 h 128.4020 dpm 0.000

2

- 07 Exhaled VOC Trap 2 168 h 41.1596 dpm 0.000

2

- 07 Cage Rinse 168 h 157981.3002 dpm 0.263

2

- 07 Nose Only Tube Ri nse 0 h 142592.3728 dpm 0.237

2

- 07 Transfer Bag Rinse 0 h 5546.1593 dpm 0.009

2

- 07 VOC Trap 1 Extract 2 1 h 1449864.3862 dpm 2.414

2

- 07 VOC Trap 1 Extract 2 3 h 1236397.9404 dpm 2.058

2

- 07 VOC Trap 1 Extract 2 5 h 1300381.4180 dpm 2.165

2

- 07 VOC Trap 1 Extract 2 7 h 1043676.1507 dpm 1.737

2

- 07 VOC Trap 1 Extract 2 12 h 1757314.8321 dpm 2.925

2

- 07 VOC Trap 1 Extract 2 24 h 473093.1094 dpm 0.788

2

- 08 Urine 12 h 22711321.3969 dpm 61989781.106 dpm 36.637

2

- 08 Urine 24 h 14234955.0469 dpm 22.96 3

2

- 08 Urine 48 h 4749402.5413 dpm 7.662

2

- 08 Urine 72 h 872040.1056 dpm 1.407

2

- 08 Urine 96 h 296626.4898 dpm 0.479

2

- 08 Urine 120 h 126834.0564 dpm 0.205

2

- 08 Urine 144 h 40832.7575 dpm 0.066

2

- 08 Urine 168 h 37836.9722 dpm 0.061

2

- 08 Feces 0 h 640897.2929 dpm 1.034

2

- 08 Feces 24 h 495745.1346 dpm 0.800

2

- 08 Feces 48 h 144626.5707 dpm 0.233

2

- 08 Feces 72 h 55263.4920 dpm 0.089

2

- 08 Feces 96 h 50940.7450 dpm 0.082

2

- 08 Feces 120 h 5006.2519 dpm 0.008

2

- 08 Feces 144 h 162346.7963 dp m 0.262

2

- 08 Feces 168 h 25891.3274 dpm 0.042

2

- 08 Carcass Digest 168 h 316429.9817 dpm 0.510

2

- 08 CO2 Trap 1 1 h 20930.6686 dpm 0.034

2

- 08 CO2 Trap 1 3 h 30695.6456 dpm 0.050

2

- 08 CO2 Trap 1 5 h 26931.3325 dpm 0.043

2

- 08 CO2 Trap 1 7 h 25999.1 264 dpm 0.042

2

- 08 CO2 Trap 1 12 h 55496.9970 dpm 0.090

2

- 08 CO2 Trap 1 24 h 118996.9798 dpm 0.192

2

- 08 CO2 Trap 1 48 h 35345.0111 dpm 0.057

2

- 08 CO2 Trap 1 72 h 3776.5574 dpm 0.006

2

- 08 CO2 Trap 1 96 h 6904.9372 dpm 0.011

2

- 08 CO2 Trap 1 120 h 3745.6389 dpm 0.006

2

- 08 CO2 Trap 1 144 h 2743.1002 dpm 0.004

2

- 08 CO2 Trap 1 168 h 1200.8666 dpm 0.002RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 26 Table 15 (contd). Total Recovery of Radioactivity in Samples from Group 2.

Subject Sample Time Sample DPM Sample DPM Sum Reco very

(%)

2

- 08 CO2 Trap 2 1 h 0.0000 dpm 0.000

2

- 08 CO2 Trap 2 3 h 142.1170 dpm 0.000

2

- 08 CO2 Trap 2 5 h 1619.0815 dpm 0.003

2

- 08 CO2 Trap 2 7 h 153.4922 dpm 0.000

2

- 08 CO2 Trap 2 12 h 0.0000 dpm 0.000

2

- 08 CO2 Trap 2 24 h 1728.9196 dpm 0.003

2

- 08 CO2 Trap 2 48 h 9895.3037 dpm 0.016

2

- 08 CO2 Trap 2 72 h 12320.9789 dpm 0.020

2

- 08 CO2 Trap 2 96 h 2520.5821 dpm 0.004

2

- 08 CO2 Trap 2 120 h 1565.1681 dpm 0.003

2

- 08 CO2 Trap 2 144 h 1515.3066 dpm 0.002

2

- 08 CO2 Trap 2 168 h 438.5894 dpm 0.001

2

- 08 Exhal ed VOC Trap 1 1 h 1951092.9162 dpm 3.147

2

- 08 Exhaled VOC Trap 1 3 h 1587367.2297 dpm 2.561

2

- 08 Exhaled VOC Trap 1 5 h 1612391.6954 dpm 2.601

2

- 08 Exhaled VOC Trap 1 7 h 1288128.7802 dpm 2.078

2

- 08 Exhaled VOC Trap 1 12 h 1904573.8148 dpm 3.072

2

- 08 Exhaled VOC Trap 1 24 h 543592.1180 dpm 0.877

2

- 08 Exhaled VOC Trap 1 48 h 137884.3251 dpm 0.222

2

- 08 Exhaled VOC Trap 1 72 h 90740.0471 dpm 0.146

2

- 08 Exhaled VOC Trap 1 96 h 34265.7827 dpm 0.055

2

- 08 Exhaled VOC Trap 1 120 h 23061.4232 dpm 0.037

2

- 08 Exhaled VOC Trap 1 144 h 18035.9248 dpm 0.029

2

- 08 Exhaled VOC Trap 1 168 h 41750.3412 dpm 0.067

2

- 08 Exhaled VOC Trap 2 1 h 0.0000 dpm 0.000

2

- 08 Exhaled VOC Trap 2 3 h 50.4669 dpm 0.000

2

- 08 Exhaled VOC Trap 2 5 h 31.9496 dpm 0.000

2

- 08 Exhaled VOC Trap 2 7 h 18.7611 dpm 0.000

2

- 08 Exhaled VOC Trap 2 12 h 0.0000 dpm 0.000

2

- 08 Exhaled VOC Trap 2 24 h 115.2074 dpm 0.000

2

- 08 Exhaled VOC Trap 2 48 h 82.2189 dpm 0.000

2

- 08 Exhaled VOC Trap 2 72 h 98.8462 dpm 0.000

2

- 08 Exhaled VOC Trap 2 96 h 336.3242 dpm 0.001

2

- 08 Exhaled VOC Trap 2 120 h 488.5097 dpm 0.001

2

- 08 Exhaled VOC Trap 2 144 h 240.6654 dpm 0.000

2

- 08 Exhaled VOC Trap 2 168 h 22.6491 dpm 0.000

2

- 08 Cage Rinse 168 h 216909.2616 dpm 0.350

2

- 08 Nose Only Tube Rinse 0 h 113796.4976 dpm 0.184

2

- 08 Transfer Bag Rinse 0 h 8402.2877 dpm 0.014

2

- 08 VOC Trap 1 Extract 2 1 h 1477793.5065 dpm 2.384

2

- 08 VOC Trap 1 Extract 2 3 h 1536316.3680 dpm 2.478

2

- 08 VOC Trap 1 Extract 2 5 h 1441963.8381 dpm 2.326

2

- 08 VO C Trap 1 Extract 2 7 h 934760.6276 dpm 1.508

2

- 08 VOC Trap 1 Extract 2 12 h 1316690.4043 dpm 2.124

2

- 08 VOC Trap 1 Extract 2 24 h 377114.9578 dpm 0.608RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 27 Table 16. Concentration (mg equiv./g) of Radioactivity in Exhaled VOC Trap Extracts from Group

2.

Subject Sample Time Conc (mg/g) LOD LOQ

2

- 05 Exhaled VOC Trap 1 1 h 0.7991

2

- 05 Exhaled VOC Trap 1 3 h 0.8499

2

- 05 Exhaled VOC Trap 1 5 h 0.8752

2

- 05 Exhaled VOC Trap 1 7 h 0.7614

2

- 05 Exhaled VOC Trap 1 12 h 0.7508

2

- 05 Exhaled VOC Tr ap 1 24 h 0.1751

2

- 05 Exhaled VOC Trap 1 48 h 0.0424

2

- 05 Exhaled VOC Trap 1 72 h 0.0001

2

- 05 Exhaled VOC Trap 1 96 h 0.0081

2

- 05 Exhaled VOC Trap 1 120 h 0.0090

2

- 05 Exhaled VOC Trap 1 144 h 0.0043

2

- 05 Exhaled VOC Trap 1 168 h 0.0040

2

- 05 Exhaled VOC Trap 2 1 h 0.0000 *

2

- 05 Exhaled VOC Trap 2 3 h 0.0000 *

2

- 05 Exhaled VOC Trap 2 5 h 0.0000 *

2

- 05 Exhaled VOC Trap 2 7 h 0.0000 *

2

- 05 Exhaled VOC Trap 2 12 h 0.0000 *

2

- 05 Exhaled VOC Trap 2 24 h 0.0000 *

2

- 05 Exhaled VOC Tr ap 2 48 h 0.0001

2

- 05 Exhaled VOC Trap 2 72 h 0.0259

2

- 05 Exhaled VOC Trap 2 96 h 0.0000 *

2

- 05 Exhaled VOC Trap 2 120 h 0.0000 *

2

- 05 Exhaled VOC Trap 2 144 h 0.0001

2

- 05 Exhaled VOC Trap 2 168 h 0.0000 *

2

- 05 VOC Trap 1 Extract 2 1 h 0.325 0

2

- 05 VOC Trap 1 Extract 2 3 h 0.3537

2

- 05 VOC Trap 1 Extract 2 5 h 0.4086

2

- 05 VOC Trap 1 Extract 2 7 h 0.2565

2

- 05 VOC Trap 1 Extract 2 12 h 0.3370

2

- 05 VOC Trap 1 Extract 2 24 h 0.0635

2

- 06 Exhaled VOC Trap 1 1 h 1.1440

2

- 06 Exhale d VOC Trap 1 3 h 0.9420

2

- 06 Exhaled VOC Trap 1 5 h 0.9796

2

- 06 Exhaled VOC Trap 1 7 h 0.8027

2

- 06 Exhaled VOC Trap 1 12 h 1.1496

2

- 06 Exhaled VOC Trap 1 24 h 0.2426

2

- 06 Exhaled VOC Trap 1 48 h 0.1935

2

- 06 Exhaled VOC Trap 1 72 h 0.0391

2

- 06 Exhaled VOC Trap 1 96 h 0.0126

2

- 06 Exhaled VOC Trap 1 120 h 0.0095

2

- 06 Exhaled VOC Trap 1 144 h 0.0070

2

- 06 Exhaled VOC Trap 1 168 h 0.0073

2

- 06 Exhaled VOC Trap 2 1 h 0.0000 *

2

- 06 Exhaled VOC Trap 2 3 h 0.0001 *

2

- 06 Exhaled VOC Trap 2 5 h 0.0000 *

2

- 06 Exhaled VOC Trap 2 7 h 0.0000 *

2

- 06 Exhaled VOC Trap 2 12 h 0.0000 *

2

- 06 Exhaled VOC Trap 2 24 h 0.0000 *

2

- 06 Exhaled VOC Trap 2 48 h 0.0002

2

- 06 Exhaled VOC Trap 2 72 h 0.0002

2

- 06 Exhaled VOC Trap 2 96 h 0.0003

* in LOD column indicates that sample DPM were less than background DPM, and in LOQ column indicates that

sample DPM was less than 3 x background.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 28 Table 16 (contd). Concentration (mg equiv./g) of Radioactivity in Exhaled VOC Trap Extracts from

Group 2

.

Subject Sample Time Conc (mg/g) LOD LOQ

2

- 06 Exhaled VOC Trap 2 120 h 0.0002

2

- 06 Exhaled VOC Trap 2 144 h 0.0002

2

- 06 Exhaled VOC Trap 2 168 h 0.0000 *

2

- 06 VOC Trap 1 Extract 2 1 h 0.4476

2

- 06 VOC Trap 1 Extract 2 3 h 0.4057

2

- 06 VOC T rap 1 Extract 2 5 h 0.4353

2

- 06 VOC Trap 1 Extract 2 7 h 0.2950

2

- 06 VOC Trap 1 Extract 2 12 h 0.5111

2

- 06 VOC Trap 1 Extract 2 24 h 0.1092

2

- 07 Exhaled VOC Trap 1 1 h 1.0217

2

- 07 Exhaled VOC Trap 1 3 h 0.9703

2

- 07 Exhaled VOC Trap 1 5 h 0.9478

2

- 07 Exhaled VOC Trap 1 7 h 1.0219

2

- 07 Exhaled VOC Trap 1 12 h 1.5578

2

- 07 Exhaled VOC Trap 1 24 h 0.4367

2

- 07 Exhaled VOC Trap 1 48 h 0.1636

2

- 07 Exhaled VOC Trap 1 72 h 0.0113

2

- 07 Exhaled VOC Trap 1 96 h 0.0084

2

- 07 Exhaled VOC Trap 1 120 h 0.0073

2

- 07 Exhaled VOC Trap 1 144 h 0.0073

2

- 07 Exhaled VOC Trap 1 168 h 0.0051

2

- 07 Exhaled VOC Trap 2 1 h 0.0000 *

2

- 07 Exhaled VOC Trap 2 3 h 0.0001

2

- 07 Exhaled VOC Trap 2 5 h 0.0000 *

2

- 07 Exhaled VOC Trap 2 7 h 0.000 0 *

2

- 07 Exhaled VOC Trap 2 12 h 0.0000 *

2

- 07 Exhaled VOC Trap 2 24 h 0.0013

2

- 07 Exhaled VOC Trap 2 48 h 0.0000 *

2

- 07 Exhaled VOC Trap 2 72 h 0.0000 *

2

- 07 Exhaled VOC Trap 2 96 h 0.0000 *

2

- 07 Exhaled VOC Trap 2 120 h 0.0000 *

2

- 07 Exhal ed VOC Trap 2 144 h 0.0001

2

- 07 Exhaled VOC Trap 2 168 h 0.0000 *

2

- 07 VOC Trap 1 Extract 2 1 h 0.5087

2

- 07 VOC Trap 1 Extract 2 3 h 0.4181

2

- 07 VOC Trap 1 Extract 2 5 h 0.4432

2

- 07 VOC Trap 1 Extract 2 7 h 0.3656

2

- 07 VOC Trap 1 Extract 2 12 h 0.6135

2

- 07 VOC Trap 1 Extract 2 24 h 0.1687

2

- 08 Exhaled VOC Trap 1 1 h 1.3818

2

- 08 Exhaled VOC Trap 1 3 h 1.1377

2

- 08 Exhaled VOC Trap 1 5 h 1.0716

2

- 08 Exhaled VOC Trap 1 7 h 0.8938

2

- 08 Exhaled VOC Trap 1 12 h 1.3258

2

- 08 Exh aled VOC Trap 1 24 h 0.3801

2

- 08 Exhaled VOC Trap 1 48 h 0.0671

2

- 08 Exhaled VOC Trap 1 72 h 0.0425

2

- 08 Exhaled VOC Trap 1 96 h 0.0154

2

- 08 Exhaled VOC Trap 1 120 h 0.0111

2

- 08 Exhaled VOC Trap 1 144 h 0.0085

2

- 08 Exhaled VOC Trap 1 168 h 0.0190

* in LOD column indicates that sample DPM were less than background DPM, and in LOQ column indicates that

sample DPM was less than 3 x background.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 29 Table 16 (contd). Concentration (mg equiv./g) of Radioactivity in Exhaled VOC Trap Extracts fr om

Group 2.

Subject Sample Time Conc (mg/g) LOD LOQ

2

- 08 Exhaled VOC Trap 2 1 h 0.0000 *

2

- 08 Exhaled VOC Trap 2 3 h 0.0000 *

2

- 08 Exhaled VOC Trap 2 5 h 0.0000 *

2

- 08 Exhaled VOC Trap 2 12 h 0.0000 *

2

- 08 Exhaled VOC Trap 2 24 h 0.0001

2

- 08 E xhaled VOC Trap 2 48 h 0.0000 *

2

- 08 Exhaled VOC Trap 2 72 h 0.0000 *

2

- 08 Exhaled VOC Trap 2 96 h 0.0002

2

- 08 Exhaled VOC Trap 2 120 h 0.0002

2

- 08 Exhaled VOC Trap 2 144 h 0.0001

2

- 08 Exhaled VOC Trap 2 168 h 0.0000 *

2

- 08 VOC Trap 1 Extrac t 2 1 h 0.5209

2

- 08 VOC Trap 1 Extract 2 3 h 0.5434

2

- 08 VOC Trap 1 Extract 2 5 h 0.5120

2

- 08 VOC Trap 1 Extract 2 7 h 0.3353

2

- 08 VOC Trap 1 Extract 2 12 h 0.4668

2

- 08 VOC Trap 1 Extract 2 24 h 0.1317

* in LOD column indicates that samp le DPM were less than background DPM, and in LOQ column indicates that

sample DPM was less than 3 x background.RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 30 Table 17. Concentration of TBA in Exhaled VOC Trap Extracts from Group 2.

Sample I.D injection Peak Area Conc. (μg/μl) Mean (μg/μl) Mean (μg/mg)

2

- 05 VOC Trap 1 1 h 1 751 0.584 0.755 0.796

2

- 05 VOC Trap 1 1 h 2 1188 0.926

2

- 06 VOC Trap 1 1 h 1 808 0.629 0.769 0.811

2

- 06 VOC Trap 1 1 h 2 1167 0.909

2

- 07 VOC Trap 1 1 h 1 997 0.777 0.800 0.843

2

- 07 VOC Trap 1 1 h 2 1057 0.824

2

- 08 VOC Trap 1 1 h 1 1430 1.11 1.09 1.149

2

- 08 VOC Trap 1 1 h 2 1366 1.06

2

- 05 VOC Trap 1 3 h 1 797 0.620 0.605 0.638

2

- 05 VOC Trap 1 3 h 2 758 0.590

2

- 06 VOC Trap 1 3 h 1 780 0.607 0.645 0.679

2

- 06 VOC Trap 1 3 h 2 876 0.682

2

- 07 VOC Trap 1 3 h 1 945 0.736 0.713 0. 751

2

- 07 VOC Trap 1 3 h 2 885 0.689

2

- 08 VOC Trap 1 3 h 1 1086 0.846 0.856 0. 902

2

- 08 VOC Trap 1 3 h 2 1112 0.867

2

- 05 VOC Trap 1 5 h 1 788 0.613 0.661 0.696

2

- 05 VOC Trap 1 5 h 2 909 0.708

2

- 06 VOC Trap 1 5 h 1 777 0.605 0.619 0.652

2

- 06 VOC Trap 1 5 h 2 813 0.633

2

- 07 VOC Trap 1 5 h 1 701 0.545 0.616 0.649

2

- 07 VOC Trap 1 5 h 2 881 0.686

2

- 08 VOC Trap 1 5 h 1 1031 0.803 0.765 0.806

2

- 08 VOC Trap 1 5 h 2 934 0.727

2

- 05 VOC Trap 1 7 h 1 730 0.568 0.533 0.561

2

- 05 VOC Trap 1 7 h 2 639 0.497

2

- 06 VOC Trap 1 7 h 1 764 0.595 0.534 0.563

2

- 06 VOC Trap 1 7 h 2 609 0.474

2

- 07 VOC Trap 1 7 h 1 946 0.737 0.729 0.768

2

- 07 VOC Trap 1 7 h 2 925 0.720

2

- 08 VOC Trap 1 7 h 1 838 0.652 0.603 0.635

2

- 08 V OC Trap 1 7 h 2 711 0.553

2

- 05 VOC Trap 1 12 h 1 627 0.488 0.497 0.524

2

- 05 VOC Trap 1 12 h 2 652 0.507

2

- 06 VOC Trap 1 12 h 1 1059 0.825 0.746 0.786

2

- 06 VOC Trap 1 12 h 2 857 0.667

2

- 07 VOC Trap 1 12 h 1 1445 1.13 1.13 1.196

2

- 07 VOC Trap 1 12 h 2 1466 1.14

2

- 08 VOC Trap 1 12 h 1 1197 0.933 0.948 0.999

2

- 08 VOC Trap 1 12 h 2 1235 0.963RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 31 Table 17 (contd). Concentration of TBA in Exhaled VOC Trap Extracts from Group 2.

Sample I.D injection Peak Area Conc. (μg/μl) Mean (μg/μl) Mea n (μg/mg)

2

- 05 VOC Trap 1 24 h 1 88 0.0665 0.0708 0.075

2

- 05 VOC Trap 1 24 h 2 99 0.0751

2

- 06 VOC Trap 1 24 h 1 116 0.0884 0.0935 0.098

2

- 06 VOC Trap 1 24 h 2 129 0.0986

2

- 07 VOC Trap 1 24 h 1 291 0.225 0.234 0.247

2

- 07 VOC Trap 1 24 h 2 314 0. 243

2

- 08 VOC Trap 1 24 h 1 258 0.199 0.207 0.218

2

- 08 VOC Trap 1 24 h 2 277 0.214

2

- 05 VOC Trap 1 48 h 1 15 0.00949 0.0107 0.011

2

- 05 VOC Trap 1 48 h 2 18 0.0118

2

- 06 VOC Trap 1 48 h 1 110 0.0837 0.0790 0.083

2

- 06 VOC Trap 1 48 h 2 98 0.0743

2

- 07 VOC Trap 1 48 h 1 17 0.0111 0.0107 0.011

2

- 07 VOC Trap 1 48 h 2 16 0.0103

2

- 08 VOC Trap 1 48 h 1 29 0.0204 0.0228 0.024

2

- 08 VOC Trap 1 48 h 2 35 0.0251

2

- 05 VOC Trap 1 72 h 1 n/a n/a n/a n/a

2

- 05 VOC Trap 1 72 h 2 n/a n/a

2

- 06 VOC Trap 1 72 h 1 26 0.0181 0.0200 0.021

2

- 06 VOC Trap 1 72 h 2 31 0.0220

2

- 07 VOC Trap 1 72 h 1 6 below LOQ below LOQ below LOQ

2

- 07 VOC Trap 1 72 h 2 6 below LOQ

2

- 08 VOC Trap 1 72 h 1 38 0.0275 0.0263 0.028

2

- 08 VOC Trap 1 72 h 2 35 0.0251

2

- 05 VOC Trap 1 96 h 1 6 below LOQ below LOQ below LOQ

2

- 05 VOC Trap 1 96 h 2 6 below LOQ

2

- 06 VOC Trap 1 96 h 1 8 below LOQ below LOQ below LOQ

2

- 06 VOC Trap 1 96 h 2 8 below LOQ

2

- 07 VOC Trap 1 96 h 1 6 below LOQ below LOQ below LOQ

2

- 07 VOC Tr ap 1 96 h 2 6 below LOQ

2

- 08 VOC Trap 1 96 h 1 12 below LOQ below LOQ below LOQ

2

- 08 VOC Trap 1 96 h 2 11 below LOQ

2

- 05 VOC Trap 1 120 h 1 7 below LOQ below LOQ below LOQ

2

- 05 VOC Trap 1 120 h 2 8 below LOQ

2

- 06 VOC Trap 1 120 h 1 7 below LO Q below LOQ below LOQ

2

- 06 VOC Trap 1 120 h 2 6 below LOQ

2

- 07 VOC Trap 1 120 h 1 6 below LOQ below LOQ below LOQ

2

- 07 VOC Trap 1 120 h 2 5 below LOQ

2

- 08 VOC Trap 1 120 h 1 9 below LOQ below LOQ below LOQ

2

- 08 VOC Trap 1 120 h 2 9 below LOQRTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 32 Table 17 (contd). Concentration of TBA in Exhaled VOC Trap Extracts from Group 2.

Sample I.D injection Peak Area Conc. (μg/μl) Mean (μg/μl) Mean (μg/mg)

2

- 05 VOC Trap 1 144 h 1 n/a n/a n/a n/a

2

- 05 VOC Trap 1 144 h 2 n/a n/a

2

- 06 VOC Trap 1 14 4 h 1 5 below LOQ below LOQ below LOQ

2

- 06 VOC Trap 1 144 h 2 n/a n/a

2

- 07 VOC Trap 1 144 h 1 6 below LOQ below LOQ below LOQ

2

- 07 VOC Trap 1 144 h 2 6 below LOQ

2

- 08 VOC Trap 1 144 h 1 7 below LOQ below LOQ below LOQ

2

- 08 VOC Trap 1 144 h 2 7 b elow LOQ

2

- 05 VOC Trap 1 168 h 1 n/a n/a n/a n/a

2

- 05 VOC Trap 1 168 h 2 n/a n/a

2

- 06 VOC Trap 1 168 h 1 7 below LOQ below LOQ below LOQ

2

- 06 VOC Trap 1 168 h 2 6 below LOQ

2

- 07 VOC Trap 1 168 h 1 n/a n/a n/a n/a

2

- 07 VOC Trap 1 168 h 2 n/a n/a

2

- 08 VOC Trap 1 168 h 1 18 0.0118 0.0126 0.013

2

- 08 VOC Trap 1 168 h 2 20 0.0134

2

- 05 VOC 1 EX2 1 h 1 368 0.285 0.284 0.300

2

- 05 VOC 1 EX2 1 h 2 366 0.284

2

- 06 VOC 1 EX2 1 h 1 536 0.417 0.409 0.431

2

- 06 VOC 1 EX2 1 h 2 516 0.401

2

- 07 VOC 1 EX2 1 h 1 549 0.427 0.441 0.464

2

- 07 VOC 1 EX2 1 h 2 585 0.455

2

- 08 VOC 1 EX2 1 h 1 603 0.469 0.441 0.464

2

- 08 VOC 1 EX2 1 h 2 531 0.413

2

- 05 VOC Trap 2 72 h 1 21 0.0142 0.0134 0.014

2

- 05 VOC Trap 2 72 h 2 19 0.0126RTI Project No.: 0209 4 08 .004 Protocol No. RTI

- 935 Final Report



### H

- 33 Table 17 (contd) . Concentration of TBA in Exhaled VOC Trap Extracts from Group 2.

Sample I.D injection Peak Area Conc. (μg/μl) Mean (μg/μl) Mean (μg/mg)

2

- 05 VOC 1 EX2 3 h 1 397 0.308 0.312 0.329

2

- 05 VOC 1 EX2 3 h 2 407 0.316

2

- 06 VOC 1 EX2 3 h 1 462 0.359 0.35 8 0.377

2

- 06 VOC 1 EX2 3 h 2 459 0.356

2

- 07 VOC 1 EX2 3 h 1 517 0.402 0.387 0.408

2

- 07 VOC 1 EX2 3 h 2 479 0.372

2

- 08 VOC 1 EX2 3 h 1 623 0.484 0.496 0.523

2

- 08 VOC 1 EX2 3 h 2 653 0.508

2

- 05 VOC 1 EX2 5 h 1 471 0.366 0.326 0.343

2

- 05 VOC 1 EX2 5 h 2 369 0.286

2

- 06 VOC 1 EX2 5 h 1 496 0.385 0.392 0.413

2

- 06 VOC 1 EX2 5 h 2 512 0.398

2

- 07 VOC 1 EX2 5 h 1 502 0.390 0.393 0.414

2

- 07 VOC 1 EX2 5 h 2 509 0.395

2

- 08 VOC 1 EX2 5 h 1 581 0.452 0.457 0.482

2

- 08 VOC 1 EX2 5 h 2 595 0.46 3

2

- 05 VOC 1 EX2 7 h 1 301 0.233 0.231 0.243

2

- 05 VOC 1 EX2 7 h 2 296 0.229

2

- 06 VOC 1 EX2 7 h 1 338 0.262 0.255 0.269

2

- 06 VOC 1 EX2 7 h 2 321 0.249

2

- 07 VOC 1 EX2 7 h 1 431 0.334 0.306 0.322

2

- 07 VOC 1 EX2 7 h 2 358 0.277

2

- 08 VOC 1 EX 2 7 h 1 361 0.280 0.292 0.308

2

- 08 VOC 1 EX2 7 h 2 392 0.304

2

- 05 VOC 1 EX2 12 h 1 392 0.304 0.294 0.310

2

- 05 VOC 1 EX2 12 h 2 366 0.284

2

- 06 VOC 1 EX2 12 h 1 572 0.445 0.450 0.474

2

- 06 VOC 1 EX2 12 h 2 586 0.456

2

- 07 VOC 1 EX2 12 h 1 669 0. 520 0.540 0.569

2

- 07 VOC 1 EX2 12 h 2 720 0.560

2

- 08 VOC 1 EX2 12 h 1 553 0.430 0.408 0.430

2

- 08 VOC 1 EX2 12 h 2 497 0.386

2

- 05 VOC 1 EX2 24 h 1 51 0.0376 0.0372 0.039

2

- 05 VOC 1 EX2 24 h 2 50 0.0368

2

- 06 VOC 1 EX2 24 h 1 101 0.0767 0.0782 0.082

2

- 06 VOC 1 EX2 24 h 2 105 0.0798

2

- 07 VOC 1 EX2 24 h 1 162 0.124 0.123 0.129

2

- 07 VOC 1 EX2 24 h 2 158 0.121

2

- 08 VOC 1 EX2 24 h 1 124 0.0946 0.0950 0.100

2

- 08 VOC 1 EX2 24 h 2 125 0.0954