# Patent

## Title
Genomics of actinoplanes utahensis

## Abstract

The present invention describes the DNA-sequence of the wild type genome as well as all genetic modifications which were introduced into the wild type—and further developed strains, based thereon. Thereby the first genotypic characterization of the developed strains, including the latest production strain, has been accomplished, accounting for the major part of the invention. Furthermore, on the basis of the determined DNA-sequences, potential genes were identified and account, combined with their functional annotation, for another part of the invention. In particular, the gene- and DNA-sequences, as well as protein-sequences derived there out, contribute to the invention which were affected by mutagenic modifications throughout the strain development process, potentially contributing to the increased production yield.

## Background
DESCRIPTION OF THE INVENTION
The gram-positive prokaryote Actinoplanes utahensis was described for the first time by John Couch in 1963 (Couch, J. N., Elisha Mitchell Sci. Soc., 1963, 79:53-70). Thereafter, in the year 1977, acarbose and its homologues were first found in the supernatant of an Actinoplanes utahensis culture (Schmidt et al., Naturwissenschaften, 1977, 64:535-536). Two years later, the medical effect of acarbose as an α-glucosidase-inhibitor within the human intestine was discovered (Caspary et al., Res. Exp. Med., 1979, 175:1-6) and within the same year, its potential application for the treatment of type-2 diabetes mellitus was propagated (Frommer et al., J. Med. Plant Res., 1979, 35:195-217).
Since 1990 the α-glucosidase-inhibitor acarbose is produced and marketed for the treatment of type-2 diabetes mellitus. Starting from the A. utahensis wild type strain the production has been continuously improved with regard to an ever increasing acarbose yield by optimization of the fermentation process as well as the production strain itself. The strain development has been driven by a multitude of mutagenesis experiments, which are primarily responsible for the raising acarbose production.
The genetic modifications in the organism, triggered by the mutagenesis experiments have so far only been recognizable by phenotypic characteristics (e.g. the increase of acarbose yield). More precisely, the genetic bases for the raising production yields have, until now, been completely unknown. However, this knowledge is of fundamental interest for the understanding of the mechanisms, leading to the rise in production. Furthermore it forms the most important prerequisite for the process of further, targeted genetic modification of the organism, optimizing A. utahensis to an even greater extend.
The present invention describes the DNA-sequence of the wild type genome as well as all genetic modifications which were introduced into the wild type- and further developed strains, based thereon. Thereby the first genotypic characterization of the developed strains, including the latest production strain, has been accomplished, accounting for the major part of the invention. Furthermore, on the basis of the determined DNA-sequences, potential genes were identified and account, combined with their functional annotation, for another part of the invention. In particular, the gene- and DNA-sequences, as well as protein-sequences derived there out which were affected by mutagenic modifications throughout the strain development process, potentially contributing to the increased production yield, contribute to the invention.
Material and Methods
As briefly described above, a series of mutagenesis experiments has been performed on the Actinoplanes utahensis wild type strain SE50-100, originally isolated from a soil sample. These experiments were aimed at the identification of mutants with an improved production of acarbose as well as other parameters, relevant for industrial production by fermentation such as high growth rate, optimized nutrient needs and consumption as well as low formation of cumbersome byproducts. Initially based on the wild type strain, further mutagenesis experiments were continuously performed on the mutant strains selected from the previous experiments. During the course of the strain development, several mutants with outstanding attributes were selected as new production strains and transferred into large scale production. Of these, seven strains were selected, including the latest production strain as well as the wild type strain, to be sequenced by Bielefeld University's Center for Biotechnology (CeBiTec) Universitätsstrasse 27, 33615 Bielefeld, Germany. Table 1 lists all seven strains that have been used during this project in the chronological order of their development.
Table 1 list all A. utahensis strains used in this study in their chronological order.












Strain Symbol
Development Order
Remark







SE50-100
(1)
wild type strain


SN223-29-47
2



C445-P47
3



SN12755-48
4



SC3687-18-43
5



SC7177-40-17
6



SN19910-37-21
7
latest production strain









Strain Cultivation
Cultivation of strains in order to check their acarbose productivity was done as described previously (Schmidt et al., Naturwissenschaften, 1977, 64:535-536). In order to isolate DNA, the Actinoplanes strains were cultivated in a two-step shake flask system. Beside inorganic salts the medium contained starch hydrolysate as carbon source and yeast extract as nitrogen source. Preculture and main culture were run for 3 days and 4 days, respectively, on a rotary shaker at 28° C. Then the biomass was collected by centrifugation.
Strain Mutagenesis
The strain development of the Acarbose producer was performed by the method of stepwise selection of higher producing strains. This method uses the process of random mutation by chemical or physical means. Chemicals used to induce mutations were either alkylating agents or intercalating dyes that serve as frameshift mutagens. Physical treatment of cells to induce mutagenesis was done with UV light of 365 nm. Fragments of the mycelium were used for mutagenesis treatment in appropriate buffer systems. After the treatment the biological material was grown for a short period in liquid medium to allow phenotypic expression of the induced alterations and then plated on agar plates. A random selection of clones that survived the mutagenesis treatment was checked for their acarbose productivity in small scale shake flask experiments. The best mutant clones obtained during a mutation cycle of this kind were chosen for the next mutation step. Several such steps of mutation and selection resulted in a gradual increase of productivity.
Preparation of Genomic DNA
The preparation of genomic DNA of A. utahensis strain SE50-110 was performed by a modification of the general described procedure (Maniatis T., Fritsch E. F., Sambrook J., Molecular Cloning—A Laboratory Manual, Cold Spring Harbor Press, 1982). The mycel of 50 mL of freshly grown culture was harvested by centrifugation (10 min., 4.000 rpm, 4° C.) in a Christ centrifuge. The pellet was washed 4 times in a buffer containing 15% sucrose (Merck KGaA, Darmstadt, Germany, cat. 7651), 25 mM TrisHCl pH 7.2 (Merck KGaA, Darmstadt, Germany, cat. 1.08382.1000), and 25 mM EDTA (Merck KGaA, Darmstadt, Germany, cat. 8418) under the same conditions. Finally the pellet was resuspended in 4.5 mL of the same buffer and lysozyme (Merck KGaA, Darmstadt, Germany, cat. 1.05281.0010) and RNAse (Qiagen, Hilden, Germany, cat. 19101) were added to final concentrations of 5 mg/mL and 50 μg/mL respectively and the mixture was incubated at 37° C. for 45 minutes. After the addition of SDS (Serva, Heidelberg, Germany, cat. 20767) and proteinase K (Qiagen, Hilden, Germany, cat. 19133) to 0.5% and 2 μg/mL final concentrations respectively, the incubation was continued at 50° C. for 5 minutes. NaCl (Merck KGaA, Darmstadt, Germany, cat. 1.06404.1000) was added to a final concentration of 300 mM and the volume adjusted with WFI to 8 mL. The lysate was subjected to three successive phenol/SEVAG extractions (SEVAG is a mixture of 24 parts chloroform [Merck KGaA, Darmstadt, Germany, cat. 1.02445.1000] and 1 part isoamylalcohol [Merck KGaA, Darmstadt, Germany, cat. 1.979.1000]) and the phenol was removed by washing the DNA solution with 10 mL SEVAG. The DNA was precipitated by the addition of 0.1 volume of 3 M sodium acetate (pH 4.8) (Merck KGaA, Darmstadt, Germany, cat. 6268) and 1 volume of cold isopropanol (Merck KGaA, Darmstadt, Germany, cat. 1.09634.1011). The DNA was pelleted by centrifugation (25 minutes, 4.000 rpm, 4° C.; Christ centrifuge) and the DNA pellet was washed thoroughly (5×) with 70% ethanol (Merck KGaA, Darmstadt, Germany, cat. 1.00983.1011) (10 minutes, 4000 rpm, 4° C.; Christ centrifuge) and air-dried. Finally the pellet was resuspended in 200 μL Tris pH 8.5 over night at 4° C. and the DNA concentration was determined by measuring the optical density at 260 nm and 280 nm. The size of the prepared DNA was analysed by subjecting an aliquot (10 μL) of the DNA solution to electrophoresis through a 1% agarose gel as quality check.
Fosmid-Library Construction
Fosmids are commonly used for preparing genomic libraries when a smaller insert size is desired. The inserts have an average size of 40 kb and are produced by random shearing, yielding a more uniform coverage of the genome than other library types. Fosmids are excellent candidates for closing gaps in a whole genome sequencing projects due to their uniform coverage. The fosmid-library construction for Actinoplanes utahensis wild type has been carried out on genomic DNA by IIT Biotech GmbH, Universitätsstr. 25, 33615 Bielefeld, Germany. For construction in E. coli EPI300 cells, the CopyControlTM Cloning System (EPICENTRE Biotechnologies, 726 Post Road, Madison, Wis. 53713, USA) has been used. The kit was obtained from Biozym Scientific GmbH, Steinbrinksweg 27, 31840 Hessisch Oldendorf, Germany.
Fosmid-Library Sequencing
Fosmid-library sequencing for Actinoplanes utahensis wild type has been carried out on a 3730xl DNA-Analyzer (Applied Biosystems, 850 Lincoln Centre Drive, Foster City, Calif. 94404, USA) by IIT Biotech GmbH, Universitätsstr. 25, 33615 Bielefeld, Germany. The device performs parallel Sanger-sequencing in 96 capillaries (Sanger et al., J. Mol. Biol., 1975, 94 (3):441-448). The resulting flowgram files were base called and stored in FASTA format. Both files were later used for gap-closure and quality assessment.
High-Throughput Genome Sequencing
Genome Sequencer FLX
The Genome Sequencer FLX (GS FLX) system (454 Life Sciences, 15 Commercial Street, Branford, Conn. 06405, USA) has been used for pyrosequencing of the A. utahensis wild type strain SE50-100 as well as the latest production strain SN19910-37-21. Two different protocols and reagent series were used on the GS FLX platform:

    
    
        1. Standard series with long paired end (PE) protocol. The genome-DNA fragment size for the PE-library construction was 2.5-3.0 kb. The protocol yields an average read length of 2×100 bases and a total number of sequenced bases of about 100 Mb.
        2. Titanium series with whole genome shotgun (WGS) protocol. The genome-DNA fragment size for the WGS-library construction was 500-800 bp. The protocol yields a read length of 400-500 bases and a total number of sequenced bases ranging from 400-600 Mb.
    
    


Details on the protocols are provided in the manufacturers manuals, namely the GS FLX Sequencing Method Manual (December 2007), GS FLX Paired End DNA Library Preparation Method Manual (December 2007), GS FLX Titanium Sequencing Method Manual (October 2008) and the GS FLX Titanium General Library Preparation Method Manual (October 2008)
Genome Analyzer IIx
The Genome Analyzer IIx (GA IIx) system (Illumina, Inc., 9885 Towne Centre Drive, San Diego, Calif. 92121, USA) including Cluster-Station and Paired-End-Module has been used for sequencing-by-synthesis of the five former productions strains SN223-29-47, C445-P47, SN12755-48, SC3687-18-43 and SC7177-40-17. For all five strains, the paired end protocol with a genome-DNA fragment size of approximately 330 bp and a read length of 2×36 bases was used. Library preparation, cluster generation and sequencing were performed according to the manufacturers manuals Paired-End sequencing Sample Preparation Guide (Part #1005063 Rev. B September 2009), Using the Paired-End Cluster Generation Kit v2 on the Cluster Station and Paired-End Module (Part #1005629 Rev. C February 2009) and Using SBS Sequencing Kit v3 on the Genome Analyzer (Part #1005637 Rev. A November 2008).
Wild Type Draft Genome Assembly
The automated assembly of all Actinoplanes utahensis wild type reads generated by the GS FLX platform was performed with the Newbler assembler software (gsAssembler version 2.0.00.22, 454 Life Science). For detailed information on the assembly algorithm see the Genome Sequencer FLX System Software Manual Part C, version 2.3 (October 2009).
Wild Type Genome Finishing
In order to close remaining gaps between contiguouse sequences (contigs) still present after the automated de novo assembly by the Newbler program, the visual assembly software package Consed (Gordon et al., Genome Research, 1998, 8:195-202) was utilized. Within the graphical user interface, primer pairs at the ends of contiguous contigs were selected. These primer pairs were then used to amplify desired sequences from clones originating from the previously constructed fosmid-library in order to bridge the gaps between contiguous contigs.
After the DNA sequence of these fosmid-reads had been determined, manual assembly of all applicable reads was performed with the aid of different program features. In detail, a fosmid-read is first aligned to the 5′ end of a contig, extending it by its 5′ remainder. Afterwards, the 3′ end of the neighboring contig is aligned to this extension, spanning the previously existing gap and joining the two contigs.
In cases were the length or quality of one fosmid-read was not sufficient to span the gap, multiple rounds of primer selection, sequencing and manual assembly were performed.
Wild Type Genome Annotation
Identification of Coding Sequences (CDS)
The potential genes and partial gene sequences on the wild type genome (see Appendix) were identified by a series of computational analysis. All utilized programs are part of the GenDB annotation-pipeline (Meyer et al., Nucleic Acids Research, 2003, 31(8):2187-95). For the identification of CDSs intrinsic, extrinsic and combined methods were applied in order to achieve optimal results.
The program responsible for the intrinsic prediction of CDSs is Glimmer (Delcher et al., Nucleic Acid Research, 1999, 27:4636-41). It first constructs a training set from CDSs with optimal characteristics taken from the genome to be analyzed. Based upon this set, an interpolated Markov Model is calculated, which is used in the actual search-run to identify all CDSs of the genomic sequence. Glimmer tends to calculate more CDSs as are actually there.
The extrinsic CDS-prediction has been carried out by CRITICA (Badger et al., Mol. Biol. Evol., 1999, 16:512-24). CRITICA first makes use of the BLASTN algorithm (Altschul et al., J. Mol. Biol., 1990, 215(3):403-10) in order to determine a list of genomic sequences which show at least slight similarity to sequences from public DNA-databases. If the translated amino acid sequence possesses a higher similarity than it would be expected based on the DNA-similarities, this is interpreted as evidence for being a conserved coding sequence. CRITICA combines these results with intrinsic analysis based on the distribution of hexa nucleotides to improve the prediction of previously unknown sequences. Despite this, CRITICA still tends to predict fewer CDS in cases were no homolog sequence is already stored in a public database.
The Reganor software (McHardy et al., Bioinformatics, 2004, 20(10):1622-31) has been used to optimize the results calculated by Glimmer and CRITICA. It combines the results of both programs and thus minimizes their respective shortcomings. Moreover, the CDS predicted by CRITICA form the basis of the combined results, complemented by the intrinsic predictions calculated by Glimmer.
Annotation and Functional Prediction
The identified open reading frames were analyzed through a variety of different software packages in order to draw conclusions from their RNA- and/or amino acid-sequences regarding their potential function. Besides their functional prediction, further characteristics and structural features have also been calculated.
Homology-based searches were applied to identify conserved sequences by means of comparison to public and/or proprietary nucleotide- and protein-databases. If a significant sequence similarity was found throughout the major section of a gene, it was concluded that the gene should have a similar function in A. utahensis. The homology-based method, which was used to annotate the gene list of Actinoplanes utahensis, is termed BLASTX (Coulson, Trends in Biotechnology, 1994, 12:76-80). BLASTX translates a given nucleotide sequence into three forward and three reverse complementary reading frames before it compares them against protein databases (e.g. the public, non-redundant protein database (nr-aa) at the National Center for Biotechnology Information (NCBI)).
Enzymatic classification has been performed on the basis of enzyme commission (EC) numbers (Webb, Edwin C., San Diego: Published for the International Union of Biochemistry and Molecular Biology by Academic Press, 1992, ISBN 0-12-227164-5. For further functional gene prediction, the cluster of orthologous groups of proteins (COG) classification system has been applied (Tatusov et al., Science, 1997, 278(5338):631-7 and Tatusov et al., Nucleic Acids Res. 2001, 29(1):22-8).
To identify potential transmembrane proteins, the software TMHMM (Krogh et al., J. Mol. Biol., 2001, 305(3):567-80 and Sonnhammer et al., Proc. Int. Conf. Intell. Syst. Mol. Biol., 1998, 6:175-82) has been utilized. It makes use of Hidden Markov Models to predict transmembrane helices and other characteristics of transmembrane proteins. With information gained thereof, membrane associated functional predictions obtain significantly stronger conclusiveness.
The software SignalP (Bendtsen et al., J. Mol. Biol., 2004, 340:783-95 and Nielsen et al., protein Engineering, 2997, 10:1-6) was used to predict the secretion capability of the identified CDSs. This is done by means of Hidden Markov Models and neural networks, searching for the appearance and position of potential signal peptide cleavage sites within the amino acid sequence. The resulting score can be interpreted as a probability measure for the secretion of the translated protein. SignalP retrieves only those proteins which are secreted by the classical signal-peptide-bound mechanisms.
In order to identify further proteins from Actinoplanes utahensis which are not secreted via the classical way, the software SecretomeP has been applied (Bendtsen et al., BMC Microbiology, 2005, 5:58). The underlying neural network has been trained with secreted proteins, known to lack signal peptides despite their occurrence in the exoproteome. The final secretion capability of the translated genes was been derived by the combined results of SignalP and SecretomeP predictions.
To reveal polycistronic transcriptional units, proprietary software has been developed which predicts jointly transcribed genes by their orientation and proximity to neighboring genes (adopted from Salgado et al., Proc. Natl. Acad. Sci. USA, 2000, 97(12):6652-7). In light of these predictions, operon structures can be determined and based upon them further sequence regions can be derived with high probability of contained promoter and operator elements.
Secondary structures of single-stranded DNA-respective RNA-molecules were calculated by the RNAshapes software (Steffen et al., Bioinformatics, 2006, 22(4):500-503). The results were used for the intrinsic prediction of transcriptional terminators which indicate operon and gene ends, respectively.
Production Strain Reference Assembly
The assembly of reads obtained for all six production strains has been achieved by mapping them onto the wild type reference genome. For this task, two different software programs were utilized, taking the two read types into account which originated from the Genome Sequencer FLX (read-length 400-500 bases WGS) and Genome Analyzer IIx (read length 2×36 bases PE) system, respectively.
The gsMapper software (version 2.3, 454 Life Science) was used to align the reads from the Genome Sequencer FLX platform against the wild type reference genome. The program implements a heuristic to find the best alignment position for each read within the reference sequence. After all reads have been aligned, multiple alignments for the reads that align contiguously to the reference are performed in order to form contigs. From the contigs' multiple alignments, consensus basecall sequences are produced using the flow-signals of the reads in the multiple alignments, resulting in quality and confidence values for each base. For detailed information on the mapping algorithm see the Genome Sequencer FLX System Software Manual Part C, version 2.3 (October 2009).
As part of the CLC Genomics Workbench (CLC bio, Finlandsgade 10-12, Katrinebjerg, 8200 Aarhus N, Denmark), the short read assembly algorithm with PE information has been used to align reads from the Genome Analyzer IIx platform against the reference genome. For detailed information on the mapping algorithm see the CLC Genomics Workbench User Manual 3.7.1.
Identification of Mutations in the Production Strains
Genetic variations between the wild type strain SE50-100 and the latest production strain SN19910-37-2 have been automatically determined during the reference assembly process by means of the gsAssembler software (version 2.3, 454 Life Science). The details of the algorithm, determining single nucleotide polymorphisms (SNPs) as well as structural variations, can be found in the Genome Sequencer FLX System Software Manual Part C, version 2.3 (October 2009).
Mutations between the wild type strain and the five former productions strains have been determined using the CLC Genomics Workbench (CLC bio, Finlandsgade 10-12 Katrinebjerg, 8200 Aarhus N, Denmark). Specialized algorithms for high-throughput data analysis of SNPs and deletion/insertion polymorphisms (DIPs) have used, described in detail in CLC Genomics Workbench User Manual 3.7.1.
Sequencing, Assembly and Annotation of the Actinoplanes utahensis Wild Type Strain
The draft genome sequence of the Actinoplanes utahensis wild type strain SE50-100 has been determined by a combination of sequencing information from three high-throughput runs. These were carried out on a Genome Sequencer FLX system, using two paired-end (PE) and one whole genome shotgun (WGS) approaches. The sequencings resulted in the successful nucleotide sequence determination of about 2 million reads, accounting for approximately 407 million sequenced bases in total (see table 2 for detailed information on the outcomes of each run).
Table 2 shows the results of the three high-throughput sequencing runs for the A. utahensis wild type strain SE50-100. Two paired-end (PE) and one whole genome shotgun (WGS) run were performed.

















Paired



Run
454 Technology
Reads
Reads
Bases



















1
Standard, PE
742,169
259,260
103,840,588


2
Standard, PE
751,570
265,457
105,329,378


3
Titanium, WGS
481,602
—
197,732,895



Total
1,975,341
524,717
406,902,861









The sequenced reads were then successfully (99.65%) assembled into 476 contiguous sequences (contigs) exceeding 500 bases in length. Considering the resulting draft genome size of 9,122,632 bases, a genome coverage of 43.88-fold has been accomplished. Due to 480,030 (91.48%) successfully mapped paired-end reads, these contigs could already be ordered and oriented into eleven scaffolds (multiple contigs whose order and orientation are known from paired-end information). Table 3 gives further inside into the success- and error-rates of the assembly process leading to the preliminary draft genome sequence of the Actinoplanes utahensis wild type strain SE50-100.
Table 3 displays the results of successfully assembled reads, bases and the inferred read error. The inferred read error is calculated from mismatches between the reads and the consensus sequence of the final assembled contigs and measures the frequency of incorrectly called bases.
















Assembled

Inferred Read


Run
454 Technology
Reads
Assembled Bases
Error







1
Standard, PE
99.58% (739,079)
98.08% (101,847,643)
0.36% (370,520) 


2
Standard, PE
99.59% (748,526)
98.18% (103,411,267)
0.35% (364,397) 


3
Titanium, WGS
99.85% (480,863)
99.33% (196,416,109)
0.52% (1,018,256)



Total
 99.65% (1,968,468)
98.72% (401,675,019)
0.44% (1,753,173)









Interestingly, the genome sequence of the previously published acarbose cluster (Wehmeier, Biocat. Biotrans., 2003, 21:279-285 and Wehmeier and Piepersberg, Appl. Microbiol. Biotechnol., 2004, 63:613-625) was not identical to the sequencing results described above. In total, 37 single nucleotide polymorphisms (SNPs) and 24 deletion/insertion polymorphisms (DIPs) were found to be artificially introduced into the wild type sequence by the former sequencing attempt (see FIG. 1). The correction of these flawed sequencings lead to a minor elongation (42 bases) of the acbC gene as well as to the correction of several temporary frameshifts within the acbE gene. This however, had no consequence on overall annotation of the gene and the whole acarbose cluster.



FIG. 1 shows former false sequencings of the acarbose cluster which were corrected by the performed high-throughput sequencing described here.
Finishing of the Draft Genome Sequence by Fosmid Library Sequencing
In order to obtain a whole genome scaffold of the wild type strain SE50-100, terminal insert sequences of 999 randomly selected fosmid clones have been determined (FIG. 2). No inconsistencies between the eleven paired-end-based scaffolds and the fosmid-library-based whole genome scaffold were found, corroborating the quality of the sequencing runs as well as the accuracy of the assembly process. In total 600 Sanger reads were derived from selected clones covering most of the remaining gaps of the draft genome. By manual assembly of these reads, 411 gaps between contigs could be bridged and closed respectively. The remaining 64 contigs form a single, circular scaffold and could not be bridged with this method due to long repetitive DNA-sequences and/or uncovered regions within the fosmid library. The resulting improved genome sequence of the A. utahensis wild type strain SE50-100 and is deposited in the appendix of this document.
FIG. 2 depicts the circular mapping of the fosmid clones (grey) used to build the genomic scaffold. The eleven scaffolds, which were based on the paired-end information, are marked in black.
Based on the improved genome sequence, a guanine-cytosine (G+C) content of 71.29% has been calculated which is typical for actinobacteria closely related to the Actinoplanes genus (Ventura et al., Microbiol. Mol. Biol. Rev., 2007, 71(3): 495-548).
Annotation of the Actinoplanes utahensis Wild Type Genome
On the foundation of the improved genome sequence, a full genome annotation has been performed, resulting in the determination of 8,027 putative coding sequences (CDS) with an average gene length of 985 nucleotides. Based thereon, Actinoplanes utahensis exhibits a coding density of 86.35% with notable G+C content difference of about 3% between coding (71.68%) and non-coding (68.70%) DNA regions. By examining the structural gene composition, 1,793 putative polycistronic transcriptional units were predicted, hosting 5,980 genes (74.50%) with an average number of 3.34 genes per operon. All nucleotide sequences as well as their amino acid translations are deposited in the appendix of this document. Table 4 summarizes the outcomes of the gene prediction process.
Table 4 shows the results of the gene prediction software for the A. utahensis wild type strain.











Gene Prediction Parameter
Value
















Coding sequences
8,027


Coding bases
7,904,275 (86.35%)


Average gene length (bp)
985


Coding G + C content (%)
71.68


Non-coding G + C content (%)
68.70


Putative monocistronic transcriptional units
2,047


Putative polycistronic transcriptional units (PTU)
1,793


Average number of genes per PTU
3.34









A variety of different programs were used to perform the functional annotation of the identified open reading frames. Due to extrinsic protein database comparisons 2,839 CDSs (35.67%) could be enzymatically characterized with an enzyme commission (EC) number. In addition 701 CDSs (8.73%), possessing typical transmembrane spanning regions, have been identified and classified as membrane-associated proteins. For a total number of 600 proteins signal peptides, and thus a high probability of being secreted into the extracellular medium, have been predicted. For additional 657 proteins, other secretion mechanisms were proposed. However, these predictions would result in an unusual high number of secreted proteins. Furthermore, the cluster of orthologous groups of proteins (COG) classification system has been applied and revealed an assignment of 3,983 (49.62%) CDSs to one or multiple COG-categories. Appendix table 9 offers a more comprehensive outline of the COG-categories and its subdivisions whereas the results of the general annotation are summarized in table 5. After full annotation, 2,684 genes (33.44%) had still no associated function. However, distant similarities to other sequences were found in public databases. For 434 (5.41%) orphan genes, not even distant related sequences were found in the databases.
Table 5 lists the results of the functional gene annotation for the A. utahensis wild type strain.











Functional Annotation Parameter
Value







CDSs with annotated function
4,909 (61.16%)


 CDSs with EC-number
2,839 (35.67%)


 CDSs with COG-category
3,983 (49.62%)


CDSs with unknown function
2,684 (33.44%)


Orphan CDSs
 434 (5.41%)


Membrane associated proteins
 701 (8.73%)


Signal peptide predicted (75% confidence)
 600 (7.47%)


Other secretion mechanism predicted (95% conf.)
 657 (8.18%)









The annotated wild type genome is shown as a circular plot in FIG. 3. In addition to the depicted genes on the forward (outmost circle) and reverse strand (second circle), the G+C content (third circle) as well as the G+C skew (forth circle) is drawn in. Furthermore, several sites of high importance are marked, including the origin of replication, the previously described trehalose (Lee et al., Appl. Microbiol. Biotechnol., 2008, 80:767-778) and acarbose clusters, an interesting protein cluster consisting of about 25 contiguous ribosomal proteins as well as the location of an integrative and conjugative element (ICE). Table 6 lists the general features of the Actinoplanes utahensis wild type genome.
FIG. 3 shows a circular genome plot of the Actinoplanes utahensis SE50-100 wild type chromosome. On the outmost circle, genes in forward orientation are depicted. The second circle hosts genes on the reverse strand. The G+C content and the G+C skew are shown on the third and fourth circle, respectively.
Table 6 lists the general features of the A. utahensis SE50-100 genome.













Feature
Genome


















Total size (bp)
9,122,632



G + C content (%)
71.29



No. of CDS
8,027



No. of orphans
434



Coding density (%)
86.35



Average gene length (bp)
985



No. of rRNAs
4 × 16S-23S-5S



No. of transposease genes
39









By means of further extrinsic database searches, the most homologous gene and the organism it originates from have been assigned to each open reading frame. Together with the detailed annotations described above, this information is listed for each CDS in appendix table 10.
For many genes, an even more detailed manual annotation has been added to the (semi-) automated information described above. These genes include, but are not limited to all elements of the acarbose cluster (Wehmeier and Pipersberg, Appl. Microbiol. Biotechnol., 2004, 63: 613-625), the trehalose cluster (Lee et al., Appl. Microbiol. Biotechnol., 2008, 80:767-778) as well as certain classes of proteins such as starch degrading- and synthesizing-enzymes, sugar epimerases, genes involved in the uptake, transport and metabolism of maltose, secreted proteins, cellulases and genes involved in nitrogen metabolism and sporulation associated genes and their protein translations.
Metabolic Potential of the A. utahensis Wild Type Strain
Through the use of annotated EC numbers, it was possible to analyze the metabolic capabilities of Actinoplanes utahensis. Mapping of the EC numbers onto canonical pathways of the Kyoto Encyclopedia of Genes and Genomes (KEGG) revealed the availability of all major pathways regarding the central metabolism such as the glycolysis, the TCA cycle and the penthose-phosphate-pathway. For the utilization of the Entner-Dudoroff-pathway however, the key enzyme phosphogluconate dehydratase is missing for the catalysis of 6-Phospho-D-gluconate to 2-Dehydro-3-deoxy-D-gluconate-6P.
Genome Sequencing of the A. utahensis Production Strains
In addition to the wild type strain SE50-100, the latest production strain SN19910-37-21 as well as five former strains were sequenced in order to reveal genetic differences responsible for the increased acarbose production in these strains. The latest strain has been sequenced on the Genome Sequencer FLX (GS FLX) system, whereas the former strains were sequenced using the Genome Analyzer IIx (GA IIx) platform solely based on paired-end data. The results are summarized in table 7. In total, 5.6 billion bases were sequenced.
Table 7 lists all sequenced A. utahensis production strains in the order of their acarbose production.















Strain Symbol
Platform
Protocol
Reads
Bases
Coverage




















SN223-29-47
GA IIx
PE
34,571,040
1,209,986,400
132.64


C445-P47
GA IIx
PE
30,360,960
1,062,633,600
116.48


SN12755-48
GA IIx
PE
29,292,960
1,025,253,600
112.39


SC3687-18-43
GA IIx
PE
28,105,200
983,682,000
107.83


SC7177-40-17
GA IIx
PE
27,332,400
956,634,000
104.86


SN19910-37-21
GS FLX
Titanium, WGS
776,085
297,036,826
32.56









Identification of Genetic Variations Between the Mutated Strains and the Wild Type
Consequent reference mapping against the previously finished wild type genome lead to the assembly of all six production strains. In addition, all genetic variations between the production strains and the wild type strain could be determined. Interestingly, no major deletion mutations had taken place, as the wild type genome is generally completely covered by the reads originating from the production strains. However, 1,826 single nucleotide polymorphisms (SNPs) and 128 deletion/insertion polymorphisms (DIPs) were discovered between the wild type genome and the latest production strain. The number of SNPs introduced into each genome, as listed in table 8, rises with the chronological development of the strain. All mutations and their exact transitions are listed in appendix table 11 together with the production strains, showing their first occurrence.
Table 8 lists the number of single nucleotide polymorphisms (SNPs) and deletion/insertion polymorphisms (DIPs) that were detected between the corresponding production strain and the A. utahensis wild type genome.














Strain Symbol
SNPs
DIPs



















SN223-29-47
428
7



C445-P47
1,040
6



SN12755-48
1,204
6



SC3687-18-43
1,331
5



SC7177-40-17
1,533
7



SN19910-37-21
1,826
128









The SNP based nucleotide transitions were not gaussian distributed but show a more than 100-fold preference for the two transition G→A and C→T. FIG. 4 depicts these findings.
FIG. 4 shows the transition frequency of SNP mutations between the wild type and the latest production strain.
By comparison of annotated gene loci against positions were SNPs and DIPs were introduced, it was found that 1,896 genes (23.62%) were affected by these mutations on the nucleotide level as can be seen in FIG. 5. Of these, 376 genes were still coding for the identical protein sequence, holding only silent mutations. On the other side, the protein sequence of 816 genes changed on individual positions, leaving the amino acid sequence unchanged for the most part. However, the 704 residuary genes were hit by mutations changing their length and/or reading frame. In detail, 429 genes were predicted to have an increased length compared to the wild type whereas 275 genes were shortened.
FIG. 5 visualizes only the 1,896 genes which were hit by a mutation event. On the outmost circle, forward oriented genes are listed. On the second circle, backward genes are depicted.
The third and forth circles represent the G+C content and the G+C skew, respectively.
Modifications of the Central Metabolism
The enzyme encoding genes which were affected by mutagenesis events are likely to have an impact on the overall metabolism as well as special pathways like the one encoding for the formation of acarbose. For this reason, these genes were mapped according to their EC numbers onto canonical pathways of the KEGG database to identify loss of functionality introduced by the mutagenesis experiments. While several enzymes of the central metabolism were affected by SNPs, only few genes were hit by mutations leading to a probable loss of function. In addition, for each of these severely changed genes at least one other gene, annotated with the same EC number, was still available, probably assisting for the knocked out version.
Modifications of the Acarbose Cluster and the Use of Former Production Strains
By sequencing of the former production strains, it was possible to trace mutations back through time to the strain they were first introduced into. This analysis was especially enlightening on the sequence of the acarbose cluster as depicted in FIG. 6. The 13 SNPs which hit the cluster were sequentially introduced as mutation experiments were executed. Two SNPs were introduced into the intragenic region between genes acbW and acbV. Furthermore, two SNPs were introduced to the acbD gene. The acbD encoding protein, an acarviosyltransferase is believed to load acarbose with maltodextrins in the extracellular space prior to reimport through the acarbose importer complex. Another mutation is located in the acbH gene which encodes the subject binding protein of the acarbose importer complex.
FIG. 6 shows the acarbose cluster in conjunction with the mutations which were introduced therein during the development of the depicted former production strains.



LIST OF ALL MUTATIONS
Table 9 lists all mutations introduced into the Actinoplanes utahensis wild type strain SE50-100 by the corresponding production strains.













Position





in





reference





wild type
Base in




strain
Reference
Occurrence in Strain
Affected















SE50-100
strain
SN223-29-47
C445-P47
SN12755-48
SC3687-18-43
SC7177-40-17
SN19910-27-21
Gene ID


















7102
G

A
A
A
A
A
cds_wt_4


11919
G
A
A
A
A
A
A
cds_wt_7


12285
G
A
A
A
A
A
A
cds_wt_7


12651
G
A
A
A
A
A
A
cds_wt_7


13511
G
A
A
A
A
A
A
cds_wt_7


46974
C
T
T
T
T
T
T
cds_wt_41


62542
C

T
T
T
T
T
cds_wt_52


64957
C
T
T
T
T
T
T
cds_wt_54


65055
C
T
T
T
T
T
T
cds_wt_54


65455
C
C/T




T/C
—


65460
C
C/T




T
—


65882
G
A
A
A
A
A
A
cds_wt_55


72685
A





A/G
—


72693
A





A/G
—


72701
A





A/G
—


73373
C

T
T
T
T
T
cds_wt_61


73467
C

T
T
T
T
T
cds_wt_61


76845
C
T
T
T
T
T
T
cds_wt_64


78965
C
T
T
T
T
T
T
cds_wt_66


80653
G

A
A
A
A
A
cds_wt_67


85503
G

A
A
A
A
A
cds_wt_70


88269
G

A
A
A
A
A
cds_wt_72


88369
G

A
A
A
A
A
cds_wt_72


89368
C
T
T
T
T
T
T
cds_wt_72


89901
C

T
T
T
T
T
cds_wt_72


90029
C

T
T
T
T
T
cds_wt_72


91949
C
T
T
T
T
T
T
cds_wt_74


93427
C
T
T
T
T
T
T
—


101351
G

A
A
A
A
A
cds_wt_82


103967
C





T
cds_wt_84


104063
C
T

T
T
T
T
cds_wt_84


110857
C

T
T
T
T
T
cds_wt_88


112637
G

A
A
A
A
A
cds_wt_90


114499
G

A
A
A
A
A
cds_wt_90


115250
G

A
A
A
A
A
cds_wt_92


115873
C

T
T
T
T
T
cds_wt_92


115983
C


T


T
cds_wt_93


117494
C

T
T
T
T
T
cds_wt_93


119344
G

A
A
A
A
A
cds_wt_94


134489
C
T
T
T
T
T
T
cds_wt_106


139515
C

T
T
T
T
T
—


146767
C
T
T
T
T
T
T
cds_wt_119


161035
G

A
A
A
A
A
cds_wt_133


172303
G

A
A
A
A
A
cds_wt_143


176176
G




A
A
cds_wt_146


178880
G





G/A
cds_wt_148


180754
C


T
T

T
cds_wt_150


180925
C


T


T
cds_wt_150


184674
G


A
A
A
A
cds_wt_154


190568
C

T
T
T
T
T
cds_wt_159


190644
C

T
T
T
T
T
cds_wt_159


191971
C

T
T
T
T
T
cds_wt_161


193584
G

A
A
A
A
A
cds_wt_162


196028
C

T
T
T
T
T
cds_wt_164


196338
C

T
T
T
T
T
—


214992
G

A
A
A
A
A
—


222841
G




A
A
cds_wt_187


222924
C




T
T
cds_wt_187


228543
C





T
cds_wt_193


247200
C



T
T
T
cds_wt_205


248952
C
T
T
T
T
T
T
cds_wt_206


253299
G





A
cds_wt_209


254551
G





A
cds_wt_210


259829
C


T
T

T
—


268705
C

T
T
T
T
T
cds_wt_224


270822
G
A
A
A
A
A
A
cds_wt_227


273723
G
A

A
A

A
cds_wt_229


276430
C

T
T
T
T
T
cds_wt_232


276561
C


T
T
T
T
cds_wt_232


283007
G
A
A
A
A
A
A
—


283062
G
A
A
A
A
A
A
—


287132
C



T
T
T
cds_wt_247


294767
G




A
A
cds_wt_253


295356
C
T
T
T
T
T
T
cds_wt_254


300028
C





T
cds_wt_256


303810
G




A
A
cds_wt_259


313837
C




T
T
cds_wt_271


316698
G




A
A
cds_wt_273


316929
G

A
A
A
A
A
cds_wt_273


325503
C

T
T
T
T
T
cds_wt_283


331088
G



A
A
A
—


340040
G


A
A

A
cds_wt_297


342987
G

A
A
A
A
A
cds_wt_299


343915
G
A
A
A
A
A
A
cds_wt_300


352688
C
T
T
T
T
T
T
cds_wt_308


353668
G

A
A
A
A
A
cds_wt_308


361019
G

A
A
A
A
A
cds_wt_314


362742
G

A
A
A
A
A
cds_wt_315


375835
C
T
T
T
T
T
T
cds_wt_329


381358
G


A
A

A
cds_wt_335


382029
G

A
A
A
A
A
cds_wt_335


391677
C
T

T
T
T
T
cds_wt_345


400166
C

T
T
T
T
T
cds_wt_350


406413
G



A
A
A
cds_wt_355


409544
G

A
A
A
A
A
cds_wt_358


416199
G





A
cds_wt_364


419938
C
T
T
T
T
T
T
cds_wt_369


425067
G




A
A
cds_wt_374


425419
G




A
A
cds_wt_374


426464
G

A
A
A
A
A
cds_wt_376


438324
C

T
T
T
T
T
cds_wt_385


446368
G

A
A
A
A
A
cds_wt_390


446984
G

A
A
A
A
A
cds_wt_392


447392
G

A
A
A
A
A
—


450492
G





A
cds_wt_395


458678
C

T
T
T
T
T
cds_wt_403


459753
C





T
—


459833
A
G

G

G
G
cds_wt_405


466505
G

A
A
A
A
A
cds_wt_416


474923
G





A
cds_wt_423


482599
G


A
A
A
A
cds_wt_430


494993
G

A
A
A
A
A
cds_wt_445


500358
C
T
T
T
T
T
T
cds_wt_449


510408
C


T
T
T
T
cds_wt_459


511515
G

A
A
A
A
A
cds_wt_460


512816
G





A
cds_wt_463


513902
C
T
T
T
T
T
T
cds_wt_464


515197
G





A
cds_wt_465


518156
G

A
A
A
A
A
cds_wt_468


533618
G
A
A
A
A
A
A
cds_wt_485


536327
C
T

T
T

T
cds_wt_488


542891
G

A
A
A
A
A
cds_wt_493


548989
C

T
T
T
T
T
cds_wt_496


553081
G

A
A
A
A
A
cds_wt_500


553332
G

A
A
A
A
A
cds_wt_500


557884
G





A
cds_wt_502


557907
G





A
cds_wt_502


567026
C
T
T
T
T
T
T
—


587166
A
G
G
G
G
G
G
cds_wt_528


590721
G





A
cds_wt_530


602225
C
T
T
T
T
T
T
cds_wt_543


604184
C





T
cds_wt_544


605918
C
T
T
T
T
T
T
cds_wt_546


621774
C




T
T
—


624361
C
T
T
T
T
T
T
—


625600
C
T
T
T
T
T
T
—


626283
G

A
A
A
A
A
—


629283
G

A
A
A
A
A
cds_wt_565


636108
C




T
T
—


648981
G

A
A
A
A
A
cds_wt_582


684062
G

A
A
A
A
A
cds_wt_613


702171
C




T
T
cds_wt_627


702650
C




T
T
cds_wt_627


703452
C


T
T
T
T
cds_wt_628


706109
C





T
cds_wt_631


711168
G


A
A
A
A
—


712462
A





A/G
cds_wt_637


714678
G


A
A
A
A
cds_wt_639


714785
G

A
A
A
A
A
cds_wt_639


717602
G


A
A
A
A
cds_wt_641


719600
G


A
A
A
A
cds_wt_643


724097
G


A
A
A
A
cds_wt_646


738525
C




T
T
cds_wt_655


744594
G





A
cds_wt_662


746362
G


A
A
A
A
cds_wt_664


750285
C

T
T
T
T
T
cds_wt_667


752663
G

A
A
A
A
A
cds_wt_670


763762
C





C/G
—


763785
G





G/C
—


763792
C





C/G
—


763951
C





C/G
—


765635
G
A
A
A
A
A
A
cds_wt_682


766073
G

A
A
A
A
A
cds_wt_682


769289
G

A
A
A
A
A
cds_wt_686


776043
C


T
T
T
T
cds_wt_692


780677
C




T
T
—


781916
C


T
T
T
T
cds_wt_698


783900
C


T
T
T
T
cds_wt_699


786653
G
A
A
A
A
A
A
cds_wt_702


795572
C
T
T
T
T
T
T
cds_wt_709


796824
G


A
A
A
A
cds_wt_710


798663
G


A
A
A
A
cds_wt_712


810530
G





A
cds_wt_724


810533
C





G
—


810556
T





T/C
cds_wt_725


810557
G





G/C
cds_wt_725


810563
C





C/T
cds_wt_725


810576
A





A/G
cds_wt_725


819250
G

A
A
A
A
A
cds_wt_730


829153
C
T
T
T
T
T
T
cds_wt_739


830171
C





T
cds_wt_740


831404
G

A
A
A
A
A
cds_wt_741


838352
G





A
cds_wt_748


842068
G

A
A
A
A
A
cds_wt_750


847887
G




A
A
cds_wt_753


861775
C
T
T
T
T
T
T
cds_wt_768


870304
G

A
A
A
A
A
cds_wt_776


880820
C
T
T
T
T
T
T
cds_wt_785


897424
C
T
T
T
T
T
T
cds_wt_794


907842
G
A
A
A
A
A
A
cds_wt_799


908987
C
T
T
T
T
T
T
—


921289
G

A
A
A
A
A
cds_wt_813


941088
G
A
A
A
A
A
A
cds_wt_829


945666
G



A
A
A
cds_wt_834


959932
C





T
cds_wt_847


964504
G

A
A
A
A
A
cds_wt_850


964558
C




T
T
cds_wt_850


970334
C
T
T
T
T
T
T
cds_wt_857


972781
C

T
T
T

T
cds_wt_860


995285
G

A
A
A
A
A
cds_wt_879


1004653
C





T
—


1006503
C
T
T
T
T
T
T
cds_wt_889


1008680
G





A
cds_wt_890


1009674
G
A
A
A
A
A
A
cds_wt_893


1009813
G





A
cds_wt_893


1023301
C

T
T
T
T
T
cds_wt_902


1028963
G

A
A
A
A
A
cds_wt_908


1030345
C
T
T
T
T
T
T
cds_wt_910


1030822
G

A
A
A
A
A
cds_wt_911


1037262
G

A
A
A
A
A
cds_wt_917


1041811
C

T
T
T
T
T
cds_wt_919


1054013
C

T
T
T
T
T
—


1055406
C
T
T
T
T
T
T
—


1082623
G


A
A

A
cds_wt_952


1085482
C




T
T
cds_wt_953


1085499
C

T
T
T
T
T
cds_wt_953


1088995
C
T
T
T
T
T
T
cds_wt_956


1097530
C


T
T
T
T
cds_wt_967


1107807
T





A
—


1117905
C




T
T
—


1119169
G




A
A
cds_wt_987


1120103
G
A




A
cds_wt_989


1120852
G
A
A
A
A
A
A
cds_wt_990


1122832
C




T
T
cds_wt_990


1123080
C

T
T
T
T
T
cds_wt_990


1128153
G

A
A
A
A
A
cds_wt_993


1130585
G

A
A
A
A
A
cds_wt_999


1145784
C




T
T
cds_wt_1014


1146897
G

A
A
A
A
A
cds_wt_1015


1153166
C





T
cds_wt_1021


1163219
C




T
T
cds_wt_1027


1163309
G

A
A
A
A
A
cds_wt_1027


1163538
G


A
A

A
cds_wt_1027


1169629
C



T
T
T
cds_wt_1032


1169882
C




T
T
cds_wt_1032


1170591
C



T
T
T
cds_wt_1032


1172808
C




T
T
cds_wt_1035


1172940
C

T
T
T
T
T
cds_wt_1035


1173551
C

T
T
T
T
T
cds_wt_1035


1179238
C

T
T
T
T
T
—


1192548
C




T
T
cds_wt_1050


1194346
C

T
T
T
T
T
cds_wt_1051


1194637
G
A
A
A
A
A
A
cds_wt_1051


1196403
C
T
T
T
T
T
T
cds_wt_1053


1208932
C





T
cds_wt_1065


1221399
C




T
T
cds_wt_1081


1222202
C

T
T
T
T
T
cds_wt_1083


1225212
C




T
T
cds_wt_1086


1242677
G

A
A
A
A
A
cds_wt_1101


1249095
C




T
T
cds_wt_1105


1250477
G





A
—


1255855
C
T
T
T
T
T
T
—


1260719
C
T

T
T

T
cds_wt_1114


1261347
C





T
cds_wt_1114


1261926
C
T
T
T
T
T
T
cds_wt_1115


1262307
G

A
A
A
A
A
cds_wt_1115


1262367
G

A
A
A
A
A
cds_wt_1115


1267545
C




T
T
cds_wt_1120


1270664
C




T
T
cds_wt_1122


1272928
C




T
T
cds_wt_1123


1273797
C




T
T
cds_wt_1124


1277491
G
A

A
A

A
cds_wt_1127


1282245
G





A
cds_wt_1131


1284346
C




T
T
cds_wt_1134


1285883
C
T
T
T
T
T
T
cds_wt_1136


1291340
C




T
T
cds_wt_1144


1300654
G

A
A
A
A
A
cds_wt_1155


1306705
C




T
T
—


1307526
C
T
T
T
T
T
T
—


1308200
C
T
T
T
T
T
T
cds_wt_1161


1315876
C




T
T
cds_wt_1170


1317961
G
A
A
A
A
A
A
cds_wt_1172


1321981
C



T
T
T
cds_wt_1176


1322304
C




T
T
cds_wt_1176


1325473
C



T
T
T
cds_wt_1179


1325983
G
A
A
A
A
A
A
cds_wt_1180


1327837
C




T
T
cds_wt_1183


1329364
C



T
T
T
cds_wt_1185


1330802
G

A
A
A
A
A
cds_wt_1186


1335338
C

T
T
T

T
cds_wt_1189


1342390
C




T
T
cds_wt_1195


1343472
A



C
C
C
cds_wt_1196


1344261
C




T
T
cds_wt_1196


1346995
C




T
T
—


1348395
G




A
A
cds_wt_1202


1351344
C




T
T
cds_wt_1204


1352745
C



T
T
T
cds_wt_1206


1356389
C



T
T
T
—


1359105
G

A
A
A
A
A
cds_wt_1210


1359182
C




T
T
cds_wt_1210


1362032
C




T
T
cds_wt_1212


1370065
G

A
A
A
A
A
cds_wt_1221


1370088
C
T
T
T
T
T
T
cds_wt_1221


1379821
C





T
cds_wt_1228


1382638
G

A
A
A
A
A
cds_wt_1232


1388800
C

T
T
T
T
T
cds_wt_1238


1404278
C
T




T
cds_wt_1248


1409508
G




A
A
cds_wt_1254


1410045
G




A
A
—


1413757
G




A
A
cds_wt_1259


1415711
G




A
A
cds_wt_1262


1415773
G




A
A
cds_wt_1262


1418132
C




T
T
cds_wt_1264


1419671
G




A
A
cds_wt_1265


1420016
C




T
T
cds_wt_1265


1428228
C

T
T
T
T
T
cds_wt_1270


1428233
C
T
T
T
T
T
T
cds_wt_1270


1435884
C




T
T
cds_wt_1279


1438355
C




T
T
cds_wt_1282


1440872
G



A
A
A
cds_wt_1282


1443149
C





T
cds_wt_1285


1450678
G

A
A
A
A
A
cds_wt_1294


1453590
G

A
A
A
A
A
cds_wt_1296


1454075
C





T
cds_wt_1297


1455191
G

A
A
A
A
A
cds_wt_1298


1463521
C


T
T
T
T
cds_wt_1303


1473239
C

T
T
T
T
T
cds_wt_1311


1474775
G




A
A
cds_wt_1312


1482297
C



T
T
T
cds_wt_1318


1494980
G




A
A
cds_wt_1326


1502729
G
A
A
A
A
A
A
cds_wt_1332


1514463
C
T




T
—


1525258
G





A
cds_wt_1357


1533361
C





T
cds_wt_1364


1545597
T





T/C
—


1563822
C

T
T
T
T
T
cds_wt_1394


1563854
C
T
T
T
T
T
T
cds_wt_1394


1565818
C





T
cds_wt_1396


1566866
C




T
T
cds_wt_1397


1570236
G




A
A
cds_wt_1398


1570636
C
T
T
T
T
T
T
cds_wt_1399


1581033
T

C
C
C
C
C
cds_wt_1411


1594972
G
A
A
A
A
A
A
cds_wt_1426


1614616
C

T
T
T
T
T
cds_wt_1441


1615586
C

T
T
T
T
T
cds_wt_1441


1616152
C

T
T
T
T
T
cds_wt_1442


1616981
C


T
T
T
T
cds_wt_1443


1617561
C


T

T
T
cds_wt_1443


1617928
C
T
T
T
T
T
T
cds_wt_1444


1622589
G
A
A
A
A
A
A
cds_wt_1447


1641656
C
T




T
cds_wt_1457


1641711
C





T
cds_wt_1457


1641953
C


T

T
T
cds_wt_1458


1648653
G

A
A
A
A
A
cds_wt_1466


1648676
G

A
A
A
A
A
cds_wt_1466


1649444
C




T
T
—


1650400
G
A
A
A
A
A
A
—


1658589
G




A
A
cds_wt_1467


1661376
G





A
cds_wt_1469


1664324
C




T
T
cds_wt_1474


1680524
C
T
T
T
T
T
T
cds_wt_1485


1682349
G


A
A
A
A
cds_wt_1485


1682842
C




T
T
cds_wt_1486


1686608
C



T
T
T
cds_wt_1490


1695571
C

T
T
T
T
T
cds_wt_1495


1699167
G

A
A
A
A
A
cds_wt_1499


1700705
G





A
cds_wt_1501


1715788
T





C
cds_wt_1517


1727072
G

A
A
A
A
A
cds_wt_1526


1747963
C
T
T
T
T
T
T
cds_wt_1546


1748448
C




T
T
cds_wt_1547


1778278
C




T
T
cds_wt_1569


1779396
C

T
T
T
T
T
cds_wt_1570


1779723
C

T
T
T
T
T
cds_wt_1570


1792608
G

A
A
A
A
A
cds_wt_1583


1793069
C




T
T
cds_wt_1584


1795610
C




T
T
cds_wt_1588


1803172
C

T
T
T
T
T
—


1805902
C
T
T
T
T
T
T
cds_wt_1595


1815104
C
T
T
T
T
T
T
cds_wt_1601


1848170
G





A
cds_wt_1640


1858054
C



T
T
T
cds_wt_1649


1874864
G

A
A
A
A
A
cds_wt_1664


1878368
C

T
T
T
T
T
cds_wt_1669


1878456
C

T
T
T
T
T
cds_wt_1669


1881797
G

A
A
A
A
A
cds_wt_1671


1881821
G

A
A
A
A
A
cds_wt_1671


1883780
G

A
A
A
A
A
cds_wt_1673


1886024
G
A
A
A
A
A
A
cds_wt_1677


1886177
G

A
A
A
A
A
cds_wt_1677


1886499
G
A
A
A
A
A
A
cds_wt_1677


1888003
G





A
cds_wt_1678


1888277
G

A
A
A
A
A
—


1891012
G

A
A
A
A
A
cds_wt_1680


1891205
C
T
T
T
T
T
T
cds_wt_1681


1891493
G

A
A
A
A
A
cds_wt_1682


1891608
G

A
A
A
A
A
cds_wt_1682


1895230
C





T
cds_wt_1683


1896121
G

A
A
A
A
A
cds_wt_1684


1898465
G

A
A
A
A
A
cds_wt_1686


1904126
G

A
A
A
A
A
cds_wt_1689


1904415
G

A
A
A
A
A
cds_wt_1689


1918600
G




A
A
cds_wt_1702


1926767
G




A
A
cds_wt_1710


1930239
G
A
A
A
A
A
A
—


1933689
G

A
A
A
A
A
cds_wt_1718


1935111
G


A
A
A
A
cds_wt_1721


1938524
G

A
A
A
A
A
cds_wt_1725


1941158
G

A
A
A
A
A
cds_wt_1729


1950155
C



T

T
cds_wt_1737


1961896
C



T
T
T
cds_wt_1749


1964247
C





T
—


1971635
A




C
C
cds_wt_1755


1973884
G

A
A
A
A
A
cds_wt_1756


1985968
G
A
A
A
A
A
A
cds_wt_1769


1986215
C





T
cds_wt_1769


1987891
C




T
T
cds_wt_1770


1987907
C




T
T
cds_wt_1770


1993612
G

A
A
A
A
A
cds_wt_1778


1997079
C

T
T
T
T
T
cds_wt_1783


2043866
G




A
A
cds_wt_1830


2043889
G




A
A
cds_wt_1830


2048938
C





T
cds_wt_1835


2058063
G

A
A
A
A
A
cds_wt_1841


2058998
G
A
A
A
A
A
A
cds_wt_1841


2060664
G

A
A
A
A
A
cds_wt_1843


2060796
G

A
A
A
A
A
cds_wt_1843


2067415
G





A
—


2067911
G
A
A
A
A
A
A
cds_wt_1851


2071535
G





A
cds_wt_1854


2077305
A





G/A
—


2077309
T





C/T
—


2077321
A





G/A
—


2077324
G





A/G
—


2077336
A





G/A
—


2077351
C





T/C
—


2077353
A





G/A
—


2077369
C





T/C
—


2077383
G
G/A
G/A
G/A
G/A
G/A
A/G
—


2077384
C
C/G
C/G
C/G
C/G
C/G
G/C
—


2077398
G
G/C
G/C
G/C
G/C
G/C
G/C
—


2077432
T





C/T
—


2077603
A





G/A
cds_wt_1862


2077615
C





A/C
cds_wt_1862


2077618
G





A/G
cds_wt_1862


2077636
T





C/T
cds_wt_1862


2077645
G





C/G
cds_wt_1862


2077651
G





C/G
cds_wt_1862


2077652
A





G/A
cds_wt_1862


2077654
A





G/A
cds_wt_1862


2077663
C





A/C
cds_wt_1862


2077669
G





C/G
cds_wt_1862


2077671
T





C/T
cds_wt_1862


2077682
C





G/C
cds_wt_1862


2077688
G





A/G
cds_wt_1862


2077691
C





G/C
cds_wt_1862


2077694
A





C/A
cds_wt_1862


2077697
G





C/G
cds_wt_1862


2077703
T





G/T
cds_wt_1862


2077715
G





A/G
cds_wt_1862


2077724
A





G/A
cds_wt_1862


2077726
C





T/C
cds_wt_1862


2077730
G





C/G
cds_wt_1862


2077733
G





A/G
cds_wt_1862


2077736
C





C/T
cds_wt_1862


2077742
G





C/G
cds_wt_1862


2077745
A





G/A
cds_wt_1862


2077751
G





A/G
cds_wt_1862


2077762
T





C/T
cds_wt_1862


2077769
T





C/T
cds_wt_1862


2077772
A





G/A
cds_wt_1862


2077775
G





A/G
cds_wt_1862


2077793
G





C/G
cds_wt_1862


2077794
A





G/A
cds_wt_1862


2077795
C





T/C
cds_wt_1862


2077805
C





T/C
cds_wt_1862


2080486
C





T
cds_wt_1864


2086608
G

A
A
A
A
A
cds_wt_1866


2096915
G

A
A
A
A
A
cds_wt_1878


2098836
C
T
T
T
T
T
T
cds_wt_1880


2099830
G





A
cds_wt_1880


2126265
C

T
T
T
T
T
—


2131730
G

A
A
A
A
A
cds_wt_1909


2135109
C





T
cds_wt_1912


2136848
C

T
T
T
T
T
—


2144528
G
A
A
A
A
A
A
cds_wt_1918


2147486
G

A
A
A
A
A
cds_wt_1921


2154132
C
T
T
T
T
T
T
cds_wt_1927


2154136
C
T
T
T
T
T
T
cds_wt_1927


2188490
C



T
T
T
cds_wt_1959


2191494
C
T
T
T
T
T
T
cds_wt_1960


2199567
G

A
A
A
A
A
cds_wt_1969


2208975
G
A
A
A
A
A
A
cds_wt_1977


2212324
G



A
A
A
cds_wt_1979


2212659
G

A
A
A
A
A
cds_wt_1980


2230643
G

A
A
A
A
A
cds_wt_1996


2235434
G

A
A
A
A
A
cds_wt_2000


2250499
C
T
T
T
T
T
T
cds_wt_2012


2259118
C


T
T

T
cds_wt_2019


2260582
G





A
cds_wt_2019


2266821
C
T
T

T
T
T
cds_wt_2023


2271223
G
A
A
A
A
A
A
cds_wt_2027


2273696
C
T
T
T
T
T
T
—


2277990
C
T




T
cds_wt_2031


2291798
C

T
T
T
T
T
cds_wt_2032


2293101
C





C/T
cds_wt_2033


2293102
G





G/T
cds_wt_2033


2293121
G
C/G
C/G
C/G
C/G
C/G
G/C
cds_wt_2033


2293139
A
C/A
C/A
C/A
C/A
C/A
A/C
cds_wt_2033


2293157
G
G/A
G/A
G/A
G/A
G/A
G/A
cds_wt_2033


2293169
G





G/C
cds_wt_2033


2293175
G





G/A
cds_wt_2033


2293176
A





A/T
cds_wt_2033


2293184
C





C/G
cds_wt_2033


2293185
G





G/A
cds_wt_2033


2293187
A





A/G
cds_wt_2033


2293196
G





A/G
cds_wt_2033


2293202
G





G/C
cds_wt_2033


2293205
G





C/G
cds_wt_2033


2293214
G





G/C
cds_wt_2033


2293219
A





A/T
cds_wt_2033


2293220
G





G/C
cds_wt_2033


2293226
G





C/G
cds_wt_2033


2293228
G





G/A
cds_wt_2033


2293234
A





A/T
cds_wt_2033


2293235
G





G/C
cds_wt_2033


2293238
G





G/C
cds_wt_2033


2293244
G





G/C
cds_wt_2033


2293247
G





A/G
cds_wt_2033


2293253
G
G/A
A
G/A
G/A
A/G
A/G
cds_wt_2033


2293268
G
G/C
C
C
C
C
C/G
cds_wt_2033


2293310
G





G/A
cds_wt_2033


2293313
G





G/C
cds_wt_2033


2293316
G





G/A
cds_wt_2033


2293319
G





G/A
cds_wt_2033


2293328
G





G/C
cds_wt_2033


2293337
G





G/C
cds_wt_2033


2293346
G





G/C
cds_wt_2033


2293352
C





C/G
cds_wt_2033


2293367
G
G/C
C/G
G/C


G/C
cds_wt_2033


2293376
C
G/C
G/C
C/G
C/G
C/G
C/G
cds_wt_2033


2293403
C





C/G
cds_wt_2033


2293463
C





C/G
cds_wt_2033


2302130
G





A
cds_wt_2039


2312744
C




T
T
cds_wt_2051


2313183
G


A
A

A
cds_wt_2051


2315988
C
T
T
T
T
T
T
cds_wt_2052


2323085
C
T
T
T
T
T
T
cds_wt_2057


2329242
C
T
T
T
T
T
T
cds_wt_2063


2332081
G

A
A
A
A
A
cds_wt_2066


2341627
C



T

T
cds_wt_2073


2347673
C
T
T
T
T
T
T
cds_wt_2076


2348681
G
A
A
A
A
A
A
cds_wt_2077


2359598
G

A
A
A
A
A
cds_wt_2088


2362060
A





G
cds_wt_2091


2368326
G




A
A
cds_wt_2097


2376154
G
A
A
A
A
A
A
cds_wt_2107


2380176
C

T
T
T
T
T
cds_wt_2113


2382542
C

T
T
T
T
T
cds_wt_2115


2382567
C

T
T
T
T
T
cds_wt_2115


2382748
C

T
T
T
T
T
—


2389975
C



T
T
T
—


2392147
C



T
T
T
cds_wt_2122


2396255
G




A
A
cds_wt_2123


2415101
G

A
A
A

A
cds_wt_2140


2422620
C



T
T
T
cds_wt_2147


2437185
G

A
A
A
A
A
cds_wt_2158


2448470
G

A
A
A
A
A
cds_wt_2169


2452760
G

A
A
A
A
A
cds_wt_2173


2456608
C



T
T
T
cds_wt_2175


2459347
C



T
T
T
cds_wt_2177


2459641
G




A
A
cds_wt_2178


2460824
C



T
T
T
cds_wt_2180


2463427
C





T
cds_wt_2182


2482048
C
T
T
T
T
T
T
cds_wt_2197


2482381
G
A
A
A
A
A
A
cds_wt_2197


2483453
C

T
T
T
T
T
cds_wt_2199


2487377
G
A
A
A
A
A
A
cds_wt_2203


2489641
G

A
A
A
A
A
cds_wt_2206


2492054
G




A
A
—


2502775
G




A
A
—


2515457
G




A
A
cds_wt_2228


2518872
G


A
A
A
A
cds_wt_2232


2530466
G

A
A
A
A
A
cds_wt_2252


2532233
G

A
A
A
A
A
cds_wt_2256


2533237
G

A
A
A
A
A
cds_wt_2259


2536306
G

A
A
A
A
A
—


2538874
G

A
A
A
A
A
cds_wt_2265


2542087
G

A
A
A
A
A
cds_wt_2270


2544720
C




T
T
cds_wt_2270


2553277
G





A
cds_wt_2277


2556662
C




T
T
cds_wt_2283


2559280
G
A
A
A
A
A
A
—


2562212
G




A
A
cds_wt_2288


2564253
G




A
A
—


2564620
G




A
A
—


2566353
G




A
A
cds_wt_2290


2572994
G

A
A
A
A
A
cds_wt_2297


2573153
C

T
T
T
T
T
cds_wt_2297


2575327
G



A

A
cds_wt_2299


2599645
G




A
A
cds_wt_2323


2599894
G




A
A
cds_wt_2324


2601319
G




A
A
cds_wt_2324


2606340
G




A
A
cds_wt_2330


2608948
G




A
A
cds_wt_2334


2610422
C




T
T
—


2614778
G





A
cds_wt_2339


2617082
G




A
A
cds_wt_2340


2618776
C

T
T
T
T
T
cds_wt_2342


2618805
C

T
T
T
T
T
cds_wt_2342


2620558
G




A
A
cds_wt_2344


2626414
A


G
G
G
G
cds_wt_2350


2636037
C




T
T
cds_wt_2357


2639682
G




A
A
cds_wt_2361


2642753
G

A
A
A
A
A
cds_wt_2364


2644984
C




T
T
cds_wt_2364


2651718
C

T
T
T
T
T
cds_wt_2367


2652733
C




T
T
cds_wt_2368


2653074
C




T
T
cds_wt_2368


2653159
C
T
T
T
T
T
T
cds_wt_2368


2653337
G

A
A
A
A
A
cds_wt_2369


2655271
C





T
cds_wt_2371


2655308
C





T
cds_wt_2371


2657059
C




T
T
cds_wt_2371


2658261
G




A
A
cds_wt_2372


2659295
C




T
T
cds_wt_2373


2663775
C




T
T
cds_wt_2376


2670944
C




T
T
cds_wt_2378


2671043
C




T
T
cds_wt_2378


2672551
C




T
T
cds_wt_2380


2676880
C
T
T
T
T
T
T
cds_wt_2382


2677056
G

A
A
A
A
A
cds_wt_2382


2677196
C
T
T
T
T
T
T
cds_wt_2382


2678066
G

A
A
A
A
A
cds_wt_2382


2679900
C




T
T
cds_wt_2383


2686364
C




T
T
cds_wt_2390


2687028
C




T
T
cds_wt_2391


2688632
C




T
T
cds_wt_2392


2694405
G





A
cds_wt_2396


2701281
G




A
A
cds_wt_2403


2712327
G




A
A
—


2720850
C
T
T
T
T
T
T
cds_wt_2418


2728435
G




A
A
cds_wt_2425


2731397
G

A
A
A
A
A
cds_wt_2428


2735550
G
G/A
G/A
G/A
G/A
G/A
G/A
—


2735576
G





G/C
—


2738768
C


T
T
T
T
cds_wt_2433


2744600
C
T
T
T
T
T
T
cds_wt_2438


2748694
C
T
T
T
T
T
T
cds_wt_2442


2756873
C

T
T
T
T
T
cds_wt_2449


2760595
G




A
A
—


2762887
G


A
A
A
A
cds_wt_2453


2763137
G

A
A
A
A
A
cds_wt_2453


2764279
C

T
T
T
T
T
cds_wt_2454


2766645
C


T
T
T
T
cds_wt_2456


2770562
C




T
T
—


2773173
C
T
T
T
T
T
T
cds_wt_2462


2774095
G

A
A
A
A
A
cds_wt_2464


2774734
G

A
A
A
A
A
cds_wt_2465


2774874
G

A
A
A
A
A
cds_wt_2465


2776314
C




T
T
cds_wt_2466


2776919
C

T
T
T
T
T
cds_wt_2467


2778598
G




A
A
cds_wt_2468


2779539
G




A
A
—


2782696
C




T
T
cds_wt_2473


2783578
C




T
T
—


2783738
C




T
T
cds_wt_2474


2785372
C




T
T
cds_wt_2475


2785531
C




T
T
cds_wt_2475


2787379
G

A
A
A
A
A
cds_wt_2476


2788015
C




T
T
cds_wt_2477


2788247
C




T
T
cds_wt_2477


2789216
C

T
T
T
T
T
cds_wt_2478


2790626
C

T
T
T
T
T
cds_wt_2479


2791577
C




T
T
cds_wt_2480


2792510
C




T
T
cds_wt_2481


2792715
G
A
A
A
A
A
A
cds_wt_2481


2797814
C
T
T
T
T
T
T
cds_wt_2487


2799953
C

T
T
T
T
T
cds_wt_2489


2800033
C




T
T
cds_wt_2489


2802765
C




T
T
—


2803789
C

T
T
T
T
T
cds_wt_2493


2804694
C

T
T
T
T
T
cds_wt_2494


2806670
C

T
T
T
T
T
cds_wt_2496


2814611
G





A
—


2815199
C





C/T
cds_wt_2502


2816239
G





A
cds_wt_2503


2821693
G



A
A
A
cds_wt_2509


2838356
C




T
T
—


2841171
T





T/A
cds_wt_2526


2841177
C





C/G
cds_wt_2526


2841183
C





C/G
cds_wt_2526


2841213
T





T/C
cds_wt_2526


2841228
C





C/G
cds_wt_2526


2841237
G





G/C
cds_wt_2526


2841251
G





G/T
cds_wt_2526


2841255
C





C/G
cds_wt_2526


2841258
G





G/T
cds_wt_2526


2841276
C
G/C

G
G
G
C/G
cds_wt_2526


2841293
C





C/G
cds_wt_2526


2841297
C





C/A
cds_wt_2526


2841303
C





C/T
cds_wt_2526


2841306
G





G/A
cds_wt_2526


2841312
T





C/T
cds_wt_2526


2841315
C





G/C
cds_wt_2526


2841322
A





A/T
cds_wt_2526


2841323
G





G/T
cds_wt_2526


2841327
C





G/C
cds_wt_2526


2841335
G





C/G
cds_wt_2526


2841357
G





C/G
cds_wt_2526


2841360
G





C/G
cds_wt_2526


2841369
G





C/G
cds_wt_2526


2841376
T





T/C
cds_wt_2526


2841377
G





T/G
cds_wt_2526


2841384
G





C/G
cds_wt_2526


2841389
T





T/C
cds_wt_2526


2841397
G





G/T
cds_wt_2526


2841398
A





C/A
cds_wt_2526


2841402
G





A/G
cds_wt_2526


2841403
G





C/G
cds_wt_2526


2841409
T





A/T
cds_wt_2526


2841413
G





T/G
cds_wt_2526


2841423
C





G/C
cds_wt_2526


2841444
G





C/G
cds_wt_2526


2841451
C





T/C
cds_wt_2526


2841477
C





G/C
cds_wt_2526


2841514
G





T/G
cds_wt_2526


2841531
A





G/A
cds_wt_2526


2841534
G





C/G
cds_wt_2526


2841536
T





A/T
cds_wt_2526


2841540
C





C/G
cds_wt_2526


2841546
G





C/G
cds_wt_2526


2841552
G





C
cds_wt_2526


2841554
T





C/T
cds_wt_2526


2841555
C





T/C
cds_wt_2526


2841562
C





T/C
cds_wt_2526


2841563
A





C/A
cds_wt_2526


2841569
A





C/A/T
cds_wt_2526


2841575
C





T/C
cds_wt_2526


2841585
C





C/G
cds_wt_2526


2841588
G





C/G
cds_wt_2526


2841594
G





A/G
cds_wt_2526


2841605
C





C/A
cds_wt_2526


2841609
C





C/G
cds_wt_2526


2841617
T





G/T
cds_wt_2526


2841636
G
C

C
C

C/G
cds_wt_2526


2841656
A





C/A
cds_wt_2526


2841660
G





C/G
cds_wt_2526


2841668
C





T/C
cds_wt_2526


2841690
G





C/G
—


2841700
G





T
—


2841701
C





T
—


2841708
G





A/G
—


2841734
T





C
—


2841735
C





T
—


2841738
G





C
—


2841744
C





G/C
—


2841747
G





A
—


2841756
C





G
—


2841759
G





C
—


2841762
C





G
—


2841776
G





T
—


2841778
T





G
—


2841783
G





C
—


2841786
A





T
—


2841789
C





T
—


2845157
G

A
A
A
A
A
cds_wt_2528


2850318
G


A
A
A
A
cds_wt_2533


2851372
G





A
cds_wt_2534


2853353
C

T
T
T
T
T
cds_wt_2535


2853817
G





A
cds_wt_2535


2868405
C
T
T
T
T
T
T
cds_wt_2548


2874011
C

T
T
T
T
T
cds_wt_2551


2875055
G

A
A
A
A
A
—


2875488
G



A
A
A
—


2876911
G
A




A
cds_wt_2553


2882407
C

T
T
T
T
T
cds_wt_2558


2888545
C

T
T
T
T
T
cds_wt_2562


2890510
C
T
T
T
T
T
T
cds_wt_2565


2892791
G





A
cds_wt_2566


2896239
G
A




A
cds_wt_2568


2896828
G
A
A
A
A
A
A
cds_wt_2568


2896833
G
A
A
A
A
A
A
cds_wt_2568


2899845
C
T
T
T
T
T
T
—


2899865
C
T
T
T
T
T
T
—


2901123
C

T
T
T
T
T
cds_wt_2572


2905469
C

T
T
T
T
T
cds_wt_2574


2912026
G
A
A
A
A
A
A
cds_wt_2580


2921100
C




T
T
cds_wt_2587


2934976
G
A
A
A
A
A
A
—


2935134
G





A
—


2935223
G



A
A
A
—


2945715
G

A
A
A
A
A
cds_wt_2611


2953167
C
T
T
T
T
T
T
cds_wt_2616


2956464
C
T
T
T
T
T
T
cds_wt_2620


2965167
G





A
cds_wt_2630


2971031
C
T
T
T
T
T
T
cds_wt_2633


2971761
C
T
T
T
T
T
T
cds_wt_2633


2973653
C
T
T
T
T
T
T
cds_wt_2634


2976296
C
T




T
cds_wt_2637


2978371
C
T
T
T
T
T
T
cds_wt_2638


2983351
G

A
A
A
A
A
cds_wt_2644


2986416
C
T
T
T
T
T
T
cds_wt_2649


2987675
C
T
T
T
T
T
T
cds_wt_2650


2988967
C
T
T
T
T
T
T
cds_wt_2650


2992035
C




T
T
cds_wt_2651


2993614
A





G
cds_wt_2652


2994097
G
A
A
A
A
A
A
cds_wt_2652


3000921
G
A
A
A
A
A
A
cds_wt_2658


3002748
G



A
A
A
cds_wt_2659


3003382
G




A
A
—


3005352
G



A
A
A
cds_wt_2662


3006201
C



T

T
cds_wt_2662


3021497
G

A
A
A
A
A
cds_wt_2674


3026718
C




T
T
—


3027132
G



A
A
A
—


3031683
C

T
T
T
T
T
—


3032969
C

T
T
T
T
T
—


3044082
G





A
cds_wt_2679


3045579
G
A
A
A
A
A
A
cds_wt_2679


3049857
G





A
cds_wt_2681


3066905
C




T
T
cds_wt_2697


3079916
T





A/T
—


3079923
C





C/T
—


3079936
G





A/G
—


3079950
A
T/A




T/A
—


3080004
C





C/T
—


3080006
A





T/A
—


3080035
C
C/T




C/T
cds_wt_2713


3080043
A
C/A
C/A
C/A
C/A
C/A
C/A
cds_wt_2713


3080063
G
C/G
C/G
C/G
C/G
C/G
C/G
cds_wt_2713


3080084
C
C/T
C/T
C/T
C/T
C/T
C/T
cds_wt_2713


3080087
G
G/C/T
G/C/T
C/G/T
G/C/T
G/T/C
C/T/G
cds_wt_2713


3080098
C




C/A
C/A
cds_wt_2713


3080128
C




T/C
T/C
cds_wt_2713


3080136
A




G/A
G/A
cds_wt_2713


3080160
C





G/C
cds_wt_2713


3080161
T





C/T
cds_wt_2713


3080163
T





C/T
cds_wt_2713


3080183
T
T/C
T/C
T/C
T/C
T/C
C/T
cds_wt_2713


3080192
G
C/G
C/G
C/G
C/G
C/G
C/G
cds_wt_2713


3080204
G
C/G
C/G
C/G
C/G
C/G
C/G
cds_wt_2713


3080237
G





C/G
cds_wt_2713


3080288
G





C/G
cds_wt_2713


3080294
G





T/G
cds_wt_2713


3080316
C





C/T
—


3080321
T

T/C
T/C
T/C
T/C
T/C
—


3080325
G
G/T
G/T
G/T
G/T
G/T
T/G
—


3080326
C
C/T
C/T
C/T
C/T
C/T
T/C
—


3080355
A
A/T
A/T
A/T
A/T
A/T
T/A
—


3080356
A





A/G
—


3080357
A
A/G
A/G
A/G
A/G
A/G
A/G
—


3080359
A





A/G
—


3080372
A
A/G
A/G
A/G
A/G
A/G
A/G
—


3080375
A





A/C
—


3080382
G





G/A
—


3080391
A





A/G
—


3080393
C





C/T
—


3080394
G





G/A
—


3080402
A





A/G
—


3080406
A





A/G
—


3080418
A





A/G
—


3080447
C





C/T
—


3080465
T





T/C
—


3080472
C





C/T
—


3080482
A





A/G
—


3080485
C





C/T
—


3080496
T





T/C
—


3082062
G





A
—


3084014
G
A
A
A
A
A
A
cds_wt_2717


3087970
C

T
T
T
T
T
cds_wt_2721


3089578
G


A
A
A
A
—


3090231
G





G/C
cds_wt_2723


3101153
G

A
A
A
A
A
cds_wt_2729


3120006
C


T
T
T
T
—


3121040
C

T
T
T
T
T
—


3125035
C

T
T
T
T
T
cds_wt_2746


3125407
C





T
cds_wt_2746


3128098
C

T
T
T
T
T
cds_wt_2749


3131738
C

T
T
T
T
T
cds_wt_2753


3133651
C

T
T
T
T
T
cds_wt_2755


3133766
G
A
A
A
A
A
A
cds_wt_2755


3137673
C




T
T
cds_wt_2759


3138173
G
A
A
A
A
A
A
cds_wt_2760


3147694
C



T
T
T
cds_wt_2765


3148142
C

T
T
T
T
T
cds_wt_2766


3148342
C

T
T
T
T
T
cds_wt_2766


3148452
C

T
T
T
T
T
cds_wt_2766


3148777
C

T
T
T
T
T
cds_wt_2766


3148960
C

T
T
T
T
T
cds_wt_2766


3149335
C

T
T
T
T
T
cds_wt_2766


3151547
C





T
—


3152279
C

T
T
T
T
T
—


3153365
G
A
A
A
A
A
A
—


3156844
C



T
T
T
cds_wt_2777


3175067
G
A
A
A
A
A
A
cds_wt_2796


3176311
C


T

T
T
cds_wt_2797


3180510
C


T
T
T
T
cds_wt_2801


3181134
C

T
T
T
T
T
cds_wt_2803


3196810
C





T
cds_wt_2815


3198674
G
A
A
A
A
A
A
cds_wt_2817


3203788
G





A
cds_wt_2823


3204764
G





A
cds_wt_2823


3208464
G


A
A
A
A
cds_wt_2826


3213080
C

T
T
T
T
T
cds_wt_2831


3214059
C




T
T
cds_wt_2832


3216019
C


T
T

T
cds_wt_2834


3216133
C





T
cds_wt_2834


3218109
C


T
T
T
T
cds_wt_2834


3226281
C




T
T
cds_wt_2841


3229241
G



A
A
A
cds_wt_2845


3230387
G



A
A
A
cds_wt_2847


3235932
C

T
T
T
T
T
cds_wt_2851


3237109
C
T
T
T
T
T
T
cds_wt_2851


3238700
C

T
T
T
T
T
cds_wt_2854


3241279
C

T
T
T
T
T
cds_wt_2857


3241643
G



A
A
A
cds_wt_2857


3245145
C



T
T
T
cds_wt_2860


3247705
G

A
A
A
A
A
cds_wt_2861


3248186
G

A
A
A
A
A
cds_wt_2862


3249319
C

T
T
T
T
T
cds_wt_2862


3251559
G

A
A
A
A
A
—


3253347
C

T
T
T
T
T
—


3253453
C

T
T
T
T
T
cds_wt_2867


3253884
G



A
A
A
—


3254464
G



A
A
A
—


3258028
G


A
A
A
A
cds_wt_2872


3258094
G


A
A

A
—


3258637
C

T
T
T
T
T
—


3259667
C


T
T

T
cds_wt_2874


3261792
G


A
A
A
A
cds_wt_2876


3262978
C
T
T
T
T
T
T
cds_wt_2876


3266668
G

A
A
A
A
A
cds_wt_2879


3266806
C
T
T
T
T
T
T
cds_wt_2879


3267341
G


A
A
A
A
cds_wt_2880


3267927
C

T
T
T
T
T
cds_wt_2880


3268140
T

C
C
C
C
C
cds_wt_2880


3269676
G

A
A
A
A
A
cds_wt_2882


3270548
G


A
A
A
A
cds_wt_2882


3275254
A

G
G
G
G
G
cds_wt_2885


3276870
C

T
T
T
T
T
cds_wt_2888


3277187
C
T
T
T
T
T
T
—


3279932
C

T
T
T
T
T
—


3285105
C

T
T
T
T
T
cds_wt_2902


3289175
C





T
cds_wt_2903


3289533
C


T
T
T
T
cds_wt_2903


3290417
G



A

A
cds_wt_2904


3290880
C


T
T
T
T
—


3291408
C
T
T
T
T
T
T
cds_wt_2906


3294618
C

T
T
T
T
T
cds_wt_2909


3295292
C

T
T
T
T
T
—


3296150
C

T
T
T
T
T
—


3296689
C

T
T
T
T
T
cds_wt_2910


3297254
C

T
T
T
T
T
cds_wt_2910


3297593
G





A
cds_wt_2910


3297802
C

T
T
T
T
T
cds_wt_2910


3297816
G





A
cds_wt_2910


3299219
C

T
T
T
T
T
—


3300457
C

T
T
T
T
T
cds_wt_2914


3300838
C
T

T
T
T
T
cds_wt_2914


3300849
C
T
T
T
T
T
T
cds_wt_2914


3307410
G

A
A
A
A
A
—


3307738
G

A
A
A
A
A
—


3309205
C

T
T
T
T
T
cds_wt_2919


3309441
G





A
cds_wt_2919


3313410
C

T
T
T
T
T
cds_wt_2923


3314734
C

T
T
T
T
T
cds_wt_2925


3315483
C

T
T
T
T
T
cds_wt_2925


3316995
C





T
cds_wt_2926


3318906
C

T
T
T
T
T
cds_wt_2927


3319964
C


T
T
T
T
cds_wt_2928


3321895
G

A
A
A
A
A
cds_wt_2929


3326784
G

A
A
A
A
A
cds_wt_2933


3327191
G

A
A
A
A
A
cds_wt_2934


3328753
C

T
T
T
T
T
cds_wt_2934


3336319
C

T
T
T
T
T
cds_wt_2941


3337206
G




A
A
cds_wt_2941


3338688
C

T
T
T
T
T
cds_wt_2943


3345311
C

T
T
T
T
T
cds_wt_2947


3346757
G




A
A
cds_wt_2947


3347355
C



T
T
T
cds_wt_2947


3347540
C



T
T
T
cds_wt_2947


3347560
C



T
T
T
cds_wt_2947


3348860
C

T
T
T
T
T
—


3349015
C



T
T
T
cds_wt_2949


3349651
C



T
T
T
cds_wt_2949


3350820
C

T
T
T
T
T
cds_wt_2951


3351613
C

T
T
T
T
T
cds_wt_2953


3353030
C


T
T
T
T
cds_wt_2954


3354271
C



T
T
T
cds_wt_2954


3354637
C



T
T
T
cds_wt_2955


3358411
G





G/A
—


3358883
G




A
A
—


3361525
G
A
A
A
A
A
A
cds_wt_2960


3363657
C

T
T
T
T
T
—


3368167
C

T
T
T
T
T
—


3368381
C





C/T
cds_wt_2966


3375301
C

T
T
T
T
T
cds_wt_2971


3381507
C





T
cds_wt_2978


3383846
C

T
T
T
T
T
cds_wt_2982


3386322
C


T
T
T
T
cds_wt_2985


3386914
G

A
A
A
A
A
cds_wt_2986


3388650
C

T
T
T
T
T
cds_wt_2988


3388663
C


T
T
T
T
cds_wt_2988


3388840
G
A
A
A
A
A
A
cds_wt_2988


3390202
G


A
A
A
A
cds_wt_2990


3391187
C

T
T
T
T
T
cds_wt_2991


3394129
C

T
T
T
T
T
cds_wt_2995


3395838
C

T
T
T
T
T
cds_wt_2996


3396692
C





T
cds_wt_2997


3397717
C

T
T
T
T
T
cds_wt_2998


3400152
C

T
T
T
T
T
cds_wt_3000


3402866
G

A
A
A
A
A
cds_wt_3004


3403044
C




T
T
cds_wt_3004


3403253
C

T
T
T
T
T
cds_wt_3004


3404959
C

T
T
T
T
T
cds_wt_3006


3405078
C
T
T
T
T
T
T
—


3409620
G



A

A
cds_wt_3009


3412544
G

A
A
A
A
A
cds_wt_3012


3415574
A
G
G
G
G
G
G
cds_wt_3016


3418558
C


T
T
T
T
—


3418906
G

A
A
A
A
A
—


3420004
C

T
T
T
T
T
cds_wt_3019


3420345
C




T
T
cds_wt_3019


3429994
C




T
T
cds_wt_3030


3430020
G
A
A
A
A
A
A
cds_wt_3030


3431269
C

T
T
T
T
T
cds_wt_3032


3432683
G



A
A
A
—


3433788
G



A
A
A
—


3434662
C

T
T
T
T
T
cds_wt_3034


3435439
G



A
A
A
cds_wt_3035


3435661
G



A
A
A
cds_wt_3035


3440357
G



A
A
A
cds_wt_3039


3441404
C




T
T
—


3453288
C





T
cds_wt_3048


3454769
G



A
A
A
cds_wt_3048


3456480
C

T
T
T
T
T
—


3457161
C



T
T
T
cds_wt_3050


3461043
C
T
T
T
T
T
T
cds_wt_3053


3461116
G

A
A
A
A
A
cds_wt_3053


3464902
C
T
T
T
T
T
T
cds_wt_3057


3469479
C

T
T
T
T
T
—


3473066
G
A

A
A

A
cds_wt_3065


3474749
C

T
T
T
T
T
—


3484337
G

A
A
A
A
A
cds_wt_3076


3484696
C

T
T
T
T
T
cds_wt_3077


3490633
G
A
A
A
A
A
A
cds_wt_3080


3511395
C


T
T
T
T
—


3517523
G
A
A
A
A
A
A
—


3518338
C

T
T
T
T
T
cds_wt_3100


3519210
C
T
T
T
T
T
T
cds_wt_3102


3519280
C
T
T
T
T
T
T
cds_wt_3102


3526212
G
A
A
A
A
A
A
cds_wt_3108


3526229
G
A
A



A
cds_wt_3108


3532172
C




T
T
cds_wt_3117


3532281
C


T
T
T
T
cds_wt_3117


3539856
C

T
T
T
T
T
cds_wt_3124


3540438
C



T
T
T
cds_wt_3124


3540952
C


T
T
T
T
cds_wt_3125


3553678
G




A
A
cds_wt_3135


3558780
G

A
A
A
A
A
cds_wt_3138


3559503
G
A
A
A
A
A
A
cds_wt_3139


3560298
T

C
C
C
C
C
cds_wt_3140


3572036
C

T
T
T
T
T
cds_wt_3152


3587529
C

T
T
T
T
T
—


3590513
C

T
T
T
T
T
cds_wt_3168


3590937
C

T
T
T
T
T
cds_wt_3168


3596971
C





T
cds_wt_3175


3601304
C

T
T
T
T
T
—


3610733
G


A
A
A
A
—


3613460
C





T
cds_wt_3186


3617806
G

A
A
A
A
A
cds_wt_3189


3618063
C
T
T
T
T
T
T
cds_wt_3189


3618082
G





A
cds_wt_3189


3621113
G

A
A
A
A
A
cds_wt_3191


3625554
C

T
T
T
T
T
cds_wt_3196


3626449
C



T
T
T
cds_wt_3198


3626548
C



T
T
T
cds_wt_3198


3627377
C
T
T
T
T
T
T
cds_wt_3199


3627686
C



T
T
T
cds_wt_3199


3628977
C

T
T
T
T
T
cds_wt_3199


3632788
C




T
T
cds_wt_3200


3647003
C


T
T
T
T
cds_wt_3215


3650159
G





A
cds_wt_3219


3658514
C




T
T
cds_wt_3227


3659174
C
T
T
T
T
T
T
cds_wt_3228


3672218
G
A
A
A
A
A
A
cds_wt_3242


3695232
G
A
A
A
A
A
A
cds_wt_3263


3702963
C

T
T
T
T
T
cds_wt_3268


3704185
C

T
T
T
T
T
cds_wt_3269


3704278
G





G/C
cds_wt_3269


3704281
T





T/G
cds_wt_3269


3704282
C





C/T
cds_wt_3269


3704293
T





T/G
cds_wt_3269


3704327
G





A/G
cds_wt_3269


3704335
C





T/C
cds_wt_3269


3704338
C





G/C
cds_wt_3269


3704340
A





C/A
cds_wt_3269


3704371
G





C/G
cds_wt_3269


3704386
T





C/T
cds_wt_3269


3704389
C





G/C
cds_wt_3269


3704395
G





C/G
cds_wt_3269


3704404
G





C/G
cds_wt_3269


3704415
C





T/C
cds_wt_3269


3704427
G
G/A
G/A
G/A
G/A
G/A
A/G
cds_wt_3269


3704428
C
C/T
T/C
C/T
C/T
C/T
T/C
cds_wt_3269


3704464
C
C/T
C/T
C/T
C/T
C/T
T/C
cds_wt_3269


3704484
A





C/A
cds_wt_3269


3704485
C





G/C
cds_wt_3269


3704491
G





C/G
cds_wt_3269


3704512
G





C/G
cds_wt_3269


3704519
A





G/A
cds_wt_3269


3704531
A





G/A
cds_wt_3269


3704534
G





C/G
cds_wt_3269


3704536
C





G/C
cds_wt_3269


3704543
C





G/C
cds_wt_3269


3704544
T





C/T
cds_wt_3269


3704554
T





T/G
cds_wt_3269


3704563
C





G/C
cds_wt_3269


3704573
A





G/A
cds_wt_3269


3704574
A





G/A
cds_wt_3269


3704577
G





C/G
cds_wt_3269


3704584
C





G/C
cds_wt_3269


3704587
G





A/G
cds_wt_3269


3704590
G





C/G
cds_wt_3269


3704605
T





C/T
cds_wt_3269


3704608
C





G/C
cds_wt_3269


3704617
T





G/T
cds_wt_3269


3704619
A





C/A
cds_wt_3269


3704620
C





A/C
cds_wt_3269


3704629
C





G/C
cds_wt_3269


3704638
C





G/C
cds_wt_3269


3704641
G





C/G
cds_wt_3269


3704647
C





G/C
cds_wt_3269


3704650
G





C/G
cds_wt_3269


3704660
C





A/C
cds_wt_3269


3704662
G





A/G
cds_wt_3269


3704668
T





C/T
cds_wt_3269


3704669
G





A/G
cds_wt_3269


3704670
T





C/T
cds_wt_3269


3704680
C





G/C
cds_wt_3269


3704684
C





G/C
cds_wt_3269


3704686
G





C/G
cds_wt_3269


3704746
G
G/C
G/C
G/C
G/C
G/C
C/G
cds_wt_3269


3704755
C
C/G
C/G
C/G
C/G
C/G
C/G
cds_wt_3269


3704767
A
A/G
A/G
A/G
A/G
A/G
A/G
cds_wt_3269


3704782
T





T/C
cds_wt_3269


3704789
A





A/G
cds_wt_3269


3704791
C





C/G
cds_wt_3269


3704806
C





C/T
cds_wt_3269


3704815
T





T/C
cds_wt_3269


3704822
C





C/G
cds_wt_3269


3704844
C





C/A
cds_wt_3269


3704851
A





A/G
cds_wt_3269


3704852
C





C/A
cds_wt_3269


3704866
T





T/C
cds_wt_3269


3704869
C





C/G
cds_wt_3269


3704875
C





C/G
cds_wt_3269


3704906
G




A
A
cds_wt_3269


3706589
C





C/G
cds_wt_3270


3706625
G





G/C
cds_wt_3270


3706641
G





G/T
cds_wt_3270


3706652
G





G/A
cds_wt_3270


3706655
C





C/G
cds_wt_3270


3706679
T





T/C
cds_wt_3270


3706715
C





C/G
cds_wt_3270


3706735
C





C/G
cds_wt_3270


3706737
A





A/G
cds_wt_3270


3706739
G





G/C
cds_wt_3270


3706784
C





C/T
cds_wt_3270


3706790
T





T/C
cds_wt_3270


3706794
A





A/G
cds_wt_3270


3706805
G





G/C
cds_wt_3270


3706822
A





A/G
cds_wt_3270


3706862
C
C/G
C/G
C/G
C/G
C/G
C/G
cds_wt_3270


3706926
G





G/T
cds_wt_3270


3706932
A





A/C
cds_wt_3270


3706940
G





G/A
cds_wt_3270


3706945
G





G/C
cds_wt_3270


3706950
T





T/A
cds_wt_3270


3706951
C





C/G
cds_wt_3270


3706958
T





T/C
cds_wt_3270


3706960
A





A/G
cds_wt_3270


3706967
C





C/G
cds_wt_3270


3706983
C





C/G
cds_wt_3270


3706985
G





G/C
cds_wt_3270


3706994
G





G/C
cds_wt_3270


3706996
A





A/C
cds_wt_3270


3707006
G





G/C
cds_wt_3270


3707012
C





C/A
cds_wt_3270


3707013
A





A/G
cds_wt_3270


3707036
C





C/G
cds_wt_3270


3711369
G




A
A
cds_wt_3273


3713575
C





T
cds_wt_3274


3724878
G

A
A
A
A
A
cds_wt_3284


3724955
G

A
A
A
A
A
cds_wt_3285


3727102
G





A
cds_wt_3287


3727130
G





A
cds_wt_3287


3727909
C

T
T
T
T
T
cds_wt_3288


3732046
C




T
T
cds_wt_3292


3733052
G
A
A
A
A
A
A
cds_wt_3293


3740023
G




A
A
cds_wt_3297


3741710
G




A
A
cds_wt_3299


3744813
G




A
A
cds_wt_3301


3750360
G




A
A
cds_wt_3306


3756113
C
T
T
T
T
T
T
cds_wt_3312


3762328
G





A
cds_wt_3318


3763755
G




A
A
cds_wt_3319


3764788
G

A
A
A
A
A
cds_wt_3320


3771690
C
T
T
T
T
T
T
cds_wt_3327


3775098
G





A
cds_wt_3331


3775718
G




A
A
cds_wt_3332


3776484
G




A
A
cds_wt_3333


3783703
T

C
C
C
C
C
cds_wt_3342


3786947
G




A
A
cds_wt_3346


3788243
C




T
T
—


3798429
G




A
A
cds_wt_3363


3799202
G

A
A
A
A
A
cds_wt_3364


3799222
G

A
A
A
A
A
cds_wt_3364


3802171
C
T
T
T
T
T
T
cds_wt_3368


3806580
C




T
T
cds_wt_3371


3825163
C





T
cds_wt_3394


3828141
G




A
A
—


3831054
G




A
A
cds_wt_3401


3831114
G

A
A
A
A
A
cds_wt_3401


3831589
C




T
T
—


3831701
G




A
A
—


3837175
G




A
A
cds_wt_3406


3841387
G




A
A
cds_wt_3410


3843222
C
T
T
T
T
T
T
cds_wt_3413


3843509
G




A
A
cds_wt_3414


3843815
G
A
A
A
A
A
A
cds_wt_3414


3844238
G




A
A
cds_wt_3414


3846121
G





A
cds_wt_3417


3846452
G




A
A
cds_wt_3417


3852914
T


C
C
C
C
cds_wt_3423


3873931
G





A
cds_wt_3442


3879758
G




A
A
cds_wt_3449


3883035
C
T
T
T
T
T
T
cds_wt_3453


3905636
C




T
T
cds_wt_3478


3919216
C

T
T
T
T
T
cds_wt_3488


3920191
G




A
A
cds_wt_3489


3928659
C

T
T
T
T
T
cds_wt_3496


3935704
C





C/T
cds_wt_3503


3941486
G



A
A
A
cds_wt_3508


3964297
G




A
A
cds_wt_3529


3965972
G


A
A
A
A
cds_wt_3530


3968608
C

T
T
T
T
T
cds_wt_3533


3983198
C

T
T
T
T
T
cds_wt_3545


3993744
G





G/A
cds_wt_3554


4002370
G

A
A
A
A
A
cds_wt_3560


4004770
C


T
T
T
T
cds_wt_3562


4006580
C

T
T
T
T
T
cds_wt_3562


4006932
G

A
A
A
A
A
cds_wt_3562


4013932
G
A
A
A
A
A
A
cds_wt_3571


4014337
T



C
C
C
cds_wt_3572


4017638
C



T
T
T
cds_wt_3574


4019106
G

A
A
A
A
A
cds_wt_3576


4020770
C

T
T
T
T
T
cds_wt_3577


4025302
C
T
T
T
T
T
T
cds_wt_3582


4027304
G



A
A
A
cds_wt_3584


4034139
G





G/T
cds_wt_3588


4043320
A




G
G
cds_wt_3599


4063459
G

A
A
A
A
A
cds_wt_3621


4072648
C

T
T
T
T
T
cds_wt_3630


4073198
C





T
cds_wt_3630


4073446
G





A
cds_wt_3630


4074524
C

T
T
T
T
T
cds_wt_3631


4081747
C



T
T
T
cds_wt_3639


4086210
A
G
G
G
G
G
G
cds_wt_3643


4089001
C


T
T

T
cds_wt_3647


4102977
C




T
T
cds_wt_3657


4104004
C




T
T
cds_wt_3658


4114649
G

A
A
A
A
A
cds_wt_3670


4117981
G

A
A
A
A
A
cds_wt_3673


4118234
C

T
T
T
T
T
—


4121337
G

A
A
A
A
A
—


4124406
G

A
A
A
A
A
cds_wt_3680


4130087
C

T
T
T
T
T
cds_wt_3683


4148633
C




T
T
cds_wt_3698


4150599
C




T
T
cds_wt_3700


4152842
C

T
T
T
T
T
cds_wt_3703


4156366
G
A
A
A
A
A
A
cds_wt_3707


4156524
G




A
A
cds_wt_3707


4157742
G
A
A
A
A
A
A
cds_wt_3708


4157795
G
A
A
A
A
A
A
cds_wt_3708


4158022
G
A
A
A
A
A
A
cds_wt_3708


4160265
G
A

A
A

A
—


4170648
C

T
T
T
T
T
cds_wt_3719


4186914
C



T

T
cds_wt_3734


4187188
G



A

A
cds_wt_3735


4192330
C

T
T
T
T
T
cds_wt_3740


4192395
C
T
T
T
T
T
T
cds_wt_3740


4194086
C
T

T
T

T
cds_wt_3741


4196729
C
T
T
T
T
T
T
cds_wt_3743


4205330
C





T
cds_wt_3752


4218066
G
A
A
A
A
A
A
cds_wt_3764


4220842
G

A
A
A
A
A
cds_wt_3768


4231722
C

T
T
T
T
T
cds_wt_3782


4251577
C

T
T
T
T
T
—


4253562
G



A
A
A
—


4255089
G

A
A
A
A
A
cds_wt_3798


4268915
C
T
T
T
T
T
T
cds_wt_3809


4284264
C
T
T
T
T
T
T
cds_wt_3820


4285070
G





A
cds_wt_3821


4286615
G





A
cds_wt_3823


4299670
C

T
T
T
T
T
—


4302785
G

A
A
A
A
A
cds_wt_3840


4302812
C





T
cds_wt_3840


4312528
G

A
A
A
A
A
cds_wt_3851


4312875
C




T
T
cds_wt_3851


4314885
G

A
A
A
A
A
cds_wt_3852


4314896
C
T
T
T
T
T
T
cds_wt_3852


4314899
G

A
A
A
A
A
cds_wt_3852


4316350
C
T
T
T
T
T
T
cds_wt_3853


4318874
C

T
T
T
T
T
—


4319625
C



T
T
T
—


4324089
G


A
A
A
A
cds_wt_3859


4325174
G

A
A
A
A
A
cds_wt_3860


4325955
G

A
A
A
A
A
cds_wt_3860


4327176
C

T
T
T
T
T
cds_wt_3861


4328372
C

T
T
T
T
T
cds_wt_3861


4329685
C

T
T
T
T
T
cds_wt_3862


4329867
C

T
T
T
T
T
cds_wt_3862


4331851
C

T
T
T
T
T
cds_wt_3862


4331916
C

T
T
T
T
T
cds_wt_3862


4334253
C





T
—


4335755
C



T
T
T
—


4340706
C




T
T
—


4343282
T





C
cds_wt_3868


4347901
C



T
T
T
cds_wt_3875


4352755
G
A
A
A
A
A
A
cds_wt_3879


4360959
G
A
A
A
A
A
A
cds_wt_3884


4361293
C



T
T
T
cds_wt_3884


4362371
C

T
T
T
T
T
cds_wt_3885


4365880
G




A
A
cds_wt_3888


4376161
C


T
T
T
T
cds_wt_3893


4376238
C

T
T
T
T
T
cds_wt_3893


4378853
G

A
A
A
A
A
—


4395837
G




A
A
cds_wt_3904


4396016
C

T
T
T
T
T
cds_wt_3904


4402736
C

T
T
T
T
T
cds_wt_3911


4406151
G




A
A
cds_wt_3914


4414580
G
A
A
A
A
A
A
—


4414632
C

T
T
T
T
T
—


4420655
C




T
T
cds_wt_3928


4433146
G





A
cds_wt_3940


4438071
G

A
A
A
A
A
—


4440571
T

C
C
C
C
C
cds_wt_3948


4440772
G




A
A
cds_wt_3949


4443633
G




A
A
cds_wt_3952


4444731
C





T
cds_wt_3953


4446487
T

C
C
C
C
C
cds_wt_3954


4463567
C
T


T

T
cds_wt_3967


4463766
G


A


A
cds_wt_3967


4467838
G





A
cds_wt_3969


4474494
G

A
A
A
A
A
cds_wt_3976


4481185
G



A
A
A
cds_wt_3981


4486135
C

T
T
T
T
T
cds_wt_3985


4487769
G





A
cds_wt_3986


4490385
G

A
A
A
A
A
cds_wt_3989


4496083
G

A
A
A
A
A
cds_wt_3998


4499876
C

T
T
T
T
T
cds_wt_4002


4503563
C
T
T
T
T
T
T
cds_wt_4006


4504062
C
T
T
T
T
T
T
cds_wt_4006


4517204
G
A
A
A
A
A
A
cds_wt_4018


4524639
G



A

A
cds_wt_4025


4524706
G





A
cds_wt_4025


4529241
G

A
A
A
A
A
cds_wt_4028


4529778
G

A
A
A
A
A
cds_wt_4029


4531772
G
A
A
A
A
A
A
cds_wt_4032


4532547
G
A
A
A
A
A
A
cds_wt_4032


4532943
G
A
A
A
A
A
A
cds_wt_4033


4533824
G
A
A
A
A
A
A
cds_wt_4034


4535251
G

A
A
A
A
A
cds_wt_4035


4537480
G

A
A
A
A
A
cds_wt_4036


4537859
G
A
A
A
A
A
A
—


4543587
G
A
A
A
A
A
A
cds_wt_4041


4547764
C




T
T
cds_wt_4046


4558815
C

T
T
T
T
T
—


4558914
C

T
T
T
T
T
cds_wt_4060


4564648
G
A
A
A
A
A
A
cds_wt_4064


4564991
G
A
A
A
A
A
A
cds_wt_4064


4573497
C

T
T
T
T
T
cds_wt_4073


4573536
C

T
T
T
T
T
cds_wt_4073


4575408
C




T
T
cds_wt_4075


4577334
C
T
T
T
T
T
T
cds_wt_4076


4580403
G





A
cds_wt_4079


4582642
T



C
C
C
—


4588387
C
A
A
A
A
A
A
cds_wt_4086


4588663
G


A
A
A
A
cds_wt_4087


4597434
C




T
T
—


4598262
C

T
T
T
T
T
cds_wt_4091


4601247
G





G/C
cds_wt_4093


4601248
T





T/C
cds_wt_4093


4603166
C




T
T
cds_wt_4094


4611131
C

T
T
T
T
T
cds_wt_4099


4614475
C

T
T
T
T
T
cds_wt_4103


4616665
G

A
A
A
A
A
cds_wt_4104


4617801
G





A
cds_wt_4106


4619989
C


T
T

T
cds_wt_4108


4621439
G
A
A
A
A
A
A
cds_wt_4109


4626601
G
A

A
A
A
A
cds_wt_4114


4630752
C


T
T
T
T
cds_wt_4116


4649811
A





A/G
cds_wt_4135


4650590
C



T
T
T
cds_wt_4136


4669551
C

T
T
T
T
T
cds_wt_4157


4670815
G





A
cds_wt_4158


4674426
G


A
A
A
A
cds_wt_4162


4677968
C

T
T
T
T
T
cds_wt_4168


4688341
C





T
cds_wt_4174


4697092
C

T
T
T
T
T
cds_wt_4179


4700003
G
A
A
A
A
A
A
cds_wt_4181


4702254
C
T
T
T
T
T
T
cds_wt_4183


4704691
C
T
T
T
T
T
T
cds_wt_4185


4704704
C
T
T
T
T
T
T
cds_wt_4185


4713174
C

T
T
T
T
T
—


4727776
G
A
A
A
A
A
A
cds_wt_4209


4736024
C





T
cds_wt_4219


4766839
C
T
T
T
T
T
T
cds_wt_4240


4769655
C
T

T
T
T
T
cds_wt_4243


4776927
G

A
A
A
A
A
cds_wt_4247


4788381
C




T
T
cds_wt_4255


4794373
C
T
T
T
T
T
T
cds_wt_4264


4799012
G
A
A
A
A
A
A
cds_wt_4268


4812357
C





T
cds_wt_4277


4813704
G





G/T
—


4813710
G





G/T
—


4813711
A





A/C
—


4814060
C



T/C

C/T
—


4814078
C





C/T
—


4814081
C





C/T
—


4814082
G





G/C
—


4814087
T





T/C
—


4818340
G




A
A
—


4820651
C




T
T
cds_wt_4283


4821863
G
A
A
A
A
A
A
—


4852073
T





T/C
—


4852080
T





C/T
cds_wt_4318


4852093
T





C/T
cds_wt_4318


4852131
G





A/G
cds_wt_4318


4852238
T



C/T
C/T
C/T
cds_wt_4318


4852271
T





T/C
cds_wt_4318


4852348
G

G/A
G/A
G/A
G/A
G/A
cds_wt_4318


4852373
T





T/C
cds_wt_4318


4858842
C
T
T
T
T
T
T
cds_wt_4320


4859921
G
A
A
A
A
A
A
cds_wt_4321


4860949
G

A
A
A
A
A
cds_wt_4321


4872907
G



A
A
A
cds_wt_4325


4879205
C




T
T
cds_wt_4332


4886810
C




T
T
cds_wt_4337


4889101
G
A
A
A
A
A
A
cds_wt_4340


4891956
C




T
T
cds_wt_4341


4896797
C

T
T
T
T
T
cds_wt_4345


4903124
G





A
cds_wt_4350


4904325
A





A/G
—


4904344
C





C/G
—


4904345
C





C/T
—


4904355
A





G
—


4904359
G





G/C
—


4904360
T





T/C
—


4908341
C

T
T
T
T
T
—


4908687
C





T
—


4911304
C





T
cds_wt_4356


4916472
C

T
T
T
T
T
cds_wt_4359


4920831
C





T
cds_wt_4362


4924034
C

T
T
T
T
T
cds_wt_4363


4924484
C

T
T
T
T
T
cds_wt_4363


4927068
C

T
T
T
T
T
cds_wt_4367


4927798
C

T
T
T
T
T
cds_wt_4367


4929941
C

T
T
T
T
T
cds_wt_4370


4930693
C


T
T
T
T
cds_wt_4370


4932671
C





T
cds_wt_4373


4933447
T





T/C
cds_wt_4374


4948234
G




A
A
cds_wt_4390


4950870
C
T
T
T
T
T
T
cds_wt_4393


4951932
C
T
T
T
T
T
T
cds_wt_4394


4953821
C
T


T

T
—


4978515
G
A




A
cds_wt_4414


4981742
C





T
cds_wt_4416


4984320
A

G
G
G
G
G
cds_wt_4417


4987021
G
A
A
A
A
A
A
cds_wt_4421


4987606
G

A
A
A
A
A
cds_wt_4421


4991441
G

A
A
A
A
A
cds_wt_4424


4992870
G
A
A
A
A
A
A
cds_wt_4425


5014441
C
T
T
T
T
T
T
cds_wt_4442


5016187
C
T
T
T
T
T
T
cds_wt_4443


5016773
C
T
T
T
T
T
T
cds_wt_4443


5025119
G





A
cds_wt_4454


5030757
C





T
cds_wt_4459


5053257
C

T
T
T
T
T
cds_wt_4482


5056010
C

T
T
T
T
T
—


5056241
C

T
T
T
T
T
cds_wt_4487


5058845
C




T
T
—


5058850
C




T
T
—


5060436
C

T
T
T
T
T
cds_wt_4489


5061245
C

T
T
T
T
T
cds_wt_4489


5062621
C

T
T
T
T
T
cds_wt_4490


5063656
C

T
T
T
T
T
cds_wt_4492


5063753
A





A/G
cds_wt_4492


5063759
T





T/G
cds_wt_4492


5063766
C





C/T
cds_wt_4492


5063772
A





A/G
cds_wt_4492


5063775
T





T/C
cds_wt_4492


5063777
A





A/G
cds_wt_4492


5063883
T





T/C
cds_wt_4492


5063900
G
G/A
G/A
G/A
G/A
G/A
G/A
cds_wt_4492


5063967
T





C/T
cds_wt_4492


5063994
C





C/G
cds_wt_4492


5063997
T





C/T
cds_wt_4492


5064000
G





C/G
cds_wt_4492


5064054
C
C/G
C/G

C/G
C/G
C/G
cds_wt_4492


5064140
T
T/A
T/A
T/A

T/A
A/T
cds_wt_4492


5064188
G
G/C
G/C
G/C
G/C
G/C
G/C
—


5064199
G
G/A
G/A
G/A
G/A
G/A
A/G
—


5064223
T
T/C
T/C
T/C
T/C
T/C
C/T
—


5064318
G
G/A

G/A
G/A
G/A
G/A
—


5064363
G





G/A
—


5064364
C





C/G
—


5064368
T
T/C
T/C
T/C
T/C
C/T
C/T
—


5066806
C

T
T
T
T
T
cds_wt_4496


5079654
C





T
cds_wt_4505


5080712
C

T
T
T
T
T
cds_wt_4505


5080760
G

A
A
A
A
A
—


5083716
C

T
T
T
T
T
cds_wt_4508


5085149
C

T
T
T
T
T
cds_wt_4509


5087053
C

T
T
T
T
T
cds_wt_4509


5089288
G

A
A
A
A
A
cds_wt_4511


5095356
C

T
T
T
T
T
cds_wt_4517


5096131
C

T
T
T
T
T
cds_wt_4517


5099106
C




T
T
cds_wt_4520


5099331
C




T
T
cds_wt_4521


5099501
C





T
cds_wt_4521


5104316
C

T
T
T
T
T
cds_wt_4525


5104896
C

T
T
T
T
T
cds_wt_4525


5105840
C

T
T
T
T
T
cds_wt_4526


5107273
C

T
T
T
T
T
cds_wt_4528


5107438
G





A
cds_wt_4528


5110964
G





A
cds_wt_4534


5111341
G


A


A
cds_wt_4534


5111525
G
A

A


A
cds_wt_4534


5113971
C

T
T
T
T
T
cds_wt_4535


5114926
C





T/C
—


5114933
C





T/C
—


5116995
G



A
A
A
cds_wt_4538


5117622
G



A
A
A
cds_wt_4538


5126463
G



A
A
A
—


5128522
G



A
A
A
cds_wt_4545


5131328
G

A
A
A
A
A
cds_wt_4547


5132598
C

T
T
T
T
T
cds_wt_4548


5133033
C

T
T
T
T
T
cds_wt_4548


5134996
G

A
A
A
A
A
cds_wt_4551


5136119
C

T
T
T
T
T
cds_wt_4552


5139533
C
T
T
T
T
T
T
cds_wt_4555


5141709
G

A
A
A
A
A
cds_wt_4556


5147647
C

T
T
T
T
T
cds_wt_4560


5158997
C

T
T
T
T
T
cds_wt_4571


5171223
G
A
A
A
A
A
A
—


5189948
C

T
T
T
T
T
cds_wt_4598


5196838
C

T
T
T
T
T
cds_wt_4603


5203530
G





A
cds_wt_4610


5204859
C

T
T
T
T
T
cds_wt_4612


5213315
G





A
cds_wt_4621


5216015
G

A
A
A
A
A
cds_wt_4624


5221083
C

T
T
T
T
T
cds_wt_4628


5229965
G

A
A
A
A
A
cds_wt_4633


5233924
G





A
cds_wt_4635


5246591
G



A
A
A
cds_wt_4645


5247914
C
T
T
T
T
T
T
cds_wt_4646


5253451
C
T




T
cds_wt_4650


5255901
C

T
T
T
T
T
cds_wt_4652


5259871
G

A
A
A
A
A
cds_wt_4654


5270798
G
A
A
A
A
A
A
cds_wt_4667


5277822
G
A

A
A

A
cds_wt_4674


5280936
C

T
T
T
T
T
cds_wt_4680


5283902
G




A
A
cds_wt_4682


5292603
C
T
T
T
T
T
T
cds_wt_4692


5309793
G

A
A
A
A
A
cds_wt_4713


5317263
G



A
A
A
cds_wt_4720


5323245
G




A
A
cds_wt_4725


5323289
C




T
T
cds_wt_4725


5323708
G




A
A
cds_wt_4726


5326705
G





A
cds_wt_4730


5339380
G
A




A
cds_wt_4742


5349635
C
T
T
T
T
T
T
cds_wt_4752


5350111
G

A
A
A
A
A
cds_wt_4752


5356868
C

T
T
T
T
T
cds_wt_4756


5363828
C




T
T
cds_wt_4763


5385564
C
T
T
T
T
T
T
cds_wt_4780


5391456
G

A
A
A
A
A
cds_wt_4787


5404805
G




A
A
cds_wt_4798


5415953
G
A
A
A
A
A
A
cds_wt_4808


5416207
C
T
T
T
T
T
T
cds_wt_4808


5419045
C

T
T
T
T
T
cds_wt_4811


5438747
G
A
A
A
A
A
A
cds_wt_4833


5457995
G

A
A
A
A
A
cds_wt_4849


5465469
C




T
T
cds_wt_4855


5481386
G




A
A
cds_wt_4866


5485106
C

T
T
T
T
T
cds_wt_4869


5500846
G

A
A
A
A
A
cds_wt_4887


5514802
C

T
T
T
T
T
cds_wt_4901


5516464
C





T
cds_wt_4902


5530664
C

T
T
T
T
T
cds_wt_4914


5537010
G
A
A
A
A
A
A
cds_wt_4920


5540526
G
A




A
—


5541233
C

T
T
T
T
T
cds_wt_4926


5543634
C

T
T
T
T
T
cds_wt_4929


5545010
C





T
cds_wt_4929


5545574
G




A
A
cds_wt_4929


5548499
C



T
T
T
cds_wt_4933


5557711
G
A

A
A

A
cds_wt_4941


5574966
C
T
T
T
T
T
T
cds_wt_4961


5576016
C

T
T
T
T
T
—


5579654
C



T
T
T
cds_wt_4970


5582215
C





C/T
cds_wt_4972


5590781
T





T/A
—


5590796
C





C/T
—


5590809
T





T/C
—


5590810
G





G/A
—


5590823
G
G/A
G/A
G/A
G/A
G/A
G/A
—


5590828
A





A/G
—


5590847
G
G/T
G/T
G/T
G/T
G/T
G/T
—


5590861
G
G/C
G/C
G/C
G/C
G/C
G/C
—


5590867
A
A/C
A/C
A/C
A/C
A/C
A/C
—


5590868
A





A/G
—


5590886
G
G/A
G/A
G/A
G/A
G/A
A/G
cds_wt_4977


5590889
T
T/C
T/C
T/C

T/C
C/T
cds_wt_4977


5590903
A





G/A
cds_wt_4977


5590909
G





T/G
cds_wt_4977


5590910
C





G/C
cds_wt_4977


5590912
G





T/G
cds_wt_4977


5590918
G





A/G
cds_wt_4977


5590923
A





G/A
cds_wt_4977


5590927
T





G/T
cds_wt_4977


5590933
G





A/G
cds_wt_4977


5590945
C





G/C
cds_wt_4977


5590960
T





C/T
cds_wt_4977


5590961
C





T/C
cds_wt_4977


5590972
T





C/T
cds_wt_4977


5590978
T





C/T
cds_wt_4977


5590981
G





C/G
cds_wt_4977


5590987
T





C/T
cds_wt_4977


5590993
C
C/G




G/C
cds_wt_4977


5591002
G





G/A
cds_wt_4977


5591008
T
C/T
T/C
T/C
C/T
T/C
C/T
cds_wt_4977


5591026
C





G/C
cds_wt_4977


5591035
C





G/C
cds_wt_4977


5591054
C
A/C
C/A
C/A
C/A
C/A
A/C
cds_wt_4977


5591063
T





C/T
cds_wt_4977


5591065
C





G/C
cds_wt_4977


5591069
A





A/G
cds_wt_4977


5591074
A





G/A
cds_wt_4977


5591077
C





A/C
cds_wt_4977


5591092
A





G/A
cds_wt_4977


5591095
G





C/G
cds_wt_4977


5591098
T





C/T
cds_wt_4977


5591115
G





A/G
cds_wt_4977


5591158
G
T/G
T/G
T/G
T/G
T/G
T/G
cds_wt_4977


5591179
G





T/G
cds_wt_4977


5591220
C


G/C
G/C
G/C
G/C
cds_wt_4977


5591226
A



G/A
G/A
G/A
cds_wt_4977


5591268
T





C/T
cds_wt_4977


5591361
C





G/C
cds_wt_4977


5591442
G





A/G
cds_wt_4977


5591445
C





T/C
cds_wt_4977


5591457
G





C/G
cds_wt_4977


5591458
A





C/A
cds_wt_4977


5591466
G





C/G
cds_wt_4977


5591469
G





A/G
cds_wt_4977


5591472
C





G/C
cds_wt_4977


5591476
A





A/G
cds_wt_4977


5591496
G





C/G
cds_wt_4977


5591499
A





C/A
cds_wt_4977


5591505
G





C/G
cds_wt_4977


5591508
T





G/T
cds_wt_4977


5591520
A





G/A
cds_wt_4977


5591532
C





T/C
cds_wt_4977


5591541
C





T/C
cds_wt_4977


5591553
G





A/G
cds_wt_4977


5591562
G





T/G
cds_wt_4977


5591565
G





C/G
cds_wt_4977


5591571
A





G/A
cds_wt_4977


5591577
A





C/A
cds_wt_4977


5591596
C





T/C
cds_wt_4977


5591598
G





C/G
cds_wt_4977


5591607
C
C/T
C/T
C/T
C/T
C/T
T/C
cds_wt_4977


5591613
T
T/C
T/C
T/C
T/C
T/C
C/T
cds_wt_4977


5591632
C





A/C
cds_wt_4977


5591635
G





G/T
cds_wt_4977


5591640
T





T/C
cds_wt_4977


5591643
C





C/T
cds_wt_4977


5591654
A





A/C
cds_wt_4977


5591656
A





A/G
—


5604643
G

A
A
A
A
A
cds_wt_4993


5612009
C



T
T
T
cds_wt_5000


5626626
C

T
T
T
T
T
—


5629121
C

T
T
T
T
T
cds_wt_5011


5631426
C




T
T
cds_wt_5015


5636810
G

A
A
A
A
A
cds_wt_5021


5657449
C
T
T
T
T
T
T
cds_wt_5035


5659036
G

A
A
A
A
A
cds_wt_5036


5692125
C


T
T
T
T
cds_wt_5065


5694507
C


T
T
T
T
cds_wt_5069


5696833
C




T
T
cds_wt_5070


5698190
C

T
T
T
T
T
—


5705110
C


T
T
T
T
cds_wt_5076


5708625
C


T
T
T
T
cds_wt_5079


5709728
C

T
T
T
T
T
cds_wt_5079


5724677
C


T
T

T
cds_wt_5088


5727195
C





T
cds_wt_5090


5728510
C




T
T
cds_wt_5091


5732980
C


T
T
T
T
cds_wt_5094


5738712
G





A
cds_wt_5097


5738846
C





T
cds_wt_5097


5745077
C





T
cds_wt_5099


5751489
C


T
T
T
T
cds_wt_5101


5751716
C





T
cds_wt_5101


5753910
C


T
T
T
T
cds_wt_5103


5755351
C





T
cds_wt_5105


5756752
C





T
cds_wt_5107


5756803
C





T
cds_wt_5107


5760058
C





G
cds_wt_5110


5760301
C





T
cds_wt_5110


5763377
C

T
T
T
T
T
cds_wt_5111


5764363
C


T
T
T
T
cds_wt_5111


5765100
C


T
T
T
T
cds_wt_5111


5765446
C


T
T
T
T
cds_wt_5111


5767623
C
T
T
T
T
T
T
cds_wt_5113


5779664
C




T
T
cds_wt_5122


5780052
C




T
T
cds_wt_5122


5781808
C


T
T
T
T
cds_wt_5123


5783477
G

A
A
A
A
A
—


5788208
C


T
T
T
T
cds_wt_5131


5790337
C


T
T
T
T
cds_wt_5133


5791404
C


T
T
T
T
cds_wt_5133


5793414
G
A
A
A
A
A
A
cds_wt_5134


5795059
C


T
T
T
T
—


5795465
G

A
A
A
A
A
cds_wt_5136


5799803
G





A
cds_wt_5140


5803395
C


T
T
T
T
cds_wt_5141


5803716
G


A
A
A
A
cds_wt_5141


5805398
G

A
A
A
A
A
cds_wt_5143


5805735
G

A
A
A
A
A
cds_wt_5143


5807160
C
T
T
T
T
T
T
cds_wt_5145


5809295
G





A
cds_wt_5147


5810587
G




A
A
—


5811908
C





T
cds_wt_5148


5813504
C


T


T
cds_wt_5149


5813556
C

T
T
T
T
T
cds_wt_5149


5834083
C





T
cds_wt_5170


5842153
G





C
cds_wt_5173


5843698
T





T/C
cds_wt_5173


5843724
C





C/A
cds_wt_5173


5843727
C





C/T
cds_wt_5173


5843751
G





G/A
cds_wt_5173


5843752
G





G/C
cds_wt_5173


5843766
A





A/G
cds_wt_5173


5843783
A





A/G
cds_wt_5173


5843786
G





G/C
cds_wt_5173


5843787
C





C/G
cds_wt_5173


5843795
G





G/C
cds_wt_5173


5843796
T





T/A
cds_wt_5173


5843797
G





G/T
cds_wt_5173


5843804
C





C/G
cds_wt_5173


5843810
C





C/T
cds_wt_5173


5843818
T





T/C
cds_wt_5173


5843819
T





T/G
cds_wt_5173


5843831
C





C/T
cds_wt_5173


5843863
G





G/T
cds_wt_5173


5843878
C





C/T
cds_wt_5173


5843881
G





G/T
cds_wt_5173


5843888
G





G/A
cds_wt_5173


5843890
G





G/C
cds_wt_5173


5843897
C





C/G
cds_wt_5173


5843899
G





G/T
cds_wt_5173


5843902
C





C/T
cds_wt_5173


5843942
C





C/T
cds_wt_5173


5843965
G





G/C
cds_wt_5173


5843980
C





C/G
cds_wt_5173


5843981
C





C/T
cds_wt_5173


5843987
C





C/G
cds_wt_5173


5843988
C





C/T
cds_wt_5173


5843992
C





C/T
cds_wt_5173


5857131
C

T
T
T
T
T
cds_wt_5188


5858663
C



T
T
T
cds_wt_5190


5879695
G

A
A
A
A
A
cds_wt_5201


5880188
T





T/C
cds_wt_5201


5880203
G





G/C
cds_wt_5201


5880223
C





C/T
cds_wt_5201


5880241
A





A/C
cds_wt_5201


5880306
C





C/T
cds_wt_5201


5880307
T





T/C
cds_wt_5201


5880349
A





A/T
cds_wt_5201


5880412
G





G/A
cds_wt_5201


5880421
C





C/T
cds_wt_5201


5880430
A





A/G
cds_wt_5201


5880517
T
T/C
T/C
T/C
T/C
T/C
T/C
cds_wt_5201


5882826
G

A
A
A
A
A
cds_wt_5202


5887657
G





C/G
cds_wt_5205


5887660
G





C/G
cds_wt_5205


5887684
T





C/T
cds_wt_5205


5887693
T





C/T
cds_wt_5205


5887696
G





C/G
cds_wt_5205


5887718
G





G/A
cds_wt_5205


5887732
G





G/C
cds_wt_5205


5887734
G





G/A
cds_wt_5205


5887765
A
G/A
G/A
G/A
G/A
G/A
A/G/C
cds_wt_5205


5887771
G





G/C
cds_wt_5205


5887774
G





G/A
cds_wt_5205


5887775
C
T/C
T/C
T/C
T/C
T/C
C/T
cds_wt_5205


5887784
T



T/C

T/C
cds_wt_5205


5887792
T
T/C
T/C

T/C

T/C
cds_wt_5205


5887810
G





G/C
cds_wt_5205


5887813
G





G/C
cds_wt_5205


5887816
G





G/C
cds_wt_5205


5887820
G
G/C
G/C
G/C
G/C
G/C
G/C
cds_wt_5205


5887824
C
C/G
C/G
C/G
C/G
C/G
C/G
cds_wt_5205


5887831
G





G/C
cds_wt_5205


5887837
C





C/T
cds_wt_5205


5887843
C





C/G
cds_wt_5205


5887892
G

A/G
A/G
A/G
A/G
A/G
cds_wt_5205


5887931
G
G/A/C
A/G
G/A
G/A
G/A
G/A/C
cds_wt_5205


5887937
G





G/C
cds_wt_5205


5887938
C





C/G
cds_wt_5205


5887942
C





C/G
cds_wt_5205


5887945
G





G/C
cds_wt_5205


5887951
G





C/G
cds_wt_5205


5887960
G





G/C
cds_wt_5205


5887969
G





G/C
cds_wt_5205


5887970
C





C/G
cds_wt_5205


5887971
A





A/G
cds_wt_5205


5887974
G





G/T
cds_wt_5205


5887975
C





C/T
cds_wt_5205


5887977
T





T/A
cds_wt_5205


5887982
C





C/A/T
cds_wt_5205


5887988
G





G/T
cds_wt_5205


5887990
C





C/G
cds_wt_5205


5888003
A





A/C
cds_wt_5205


5888004
A





A/G
cds_wt_5205


5888005
A





A/G
cds_wt_5205


5888026
C





C/T
cds_wt_5205


5888050
G





G/C
cds_wt_5205


5888053
G





G/C
cds_wt_5205


5888056
G





G/C
cds_wt_5205


5888083
G





G/C
cds_wt_5205


5888092
C





C/G
cds_wt_5205


5888095
C





C/G
cds_wt_5205


5888102
G





G/A
cds_wt_5205


5888103
T





T/C
cds_wt_5205


5888122
C





C/T
cds_wt_5205


5888143
G





G/C
cds_wt_5205


5890782
G





G/C
cds_wt_5206


5890839
T
T/C
T/C
T/C
T/C
T/C
C/T
cds_wt_5206


5890849
T





A/T
cds_wt_5206


5890851
C





G/C
cds_wt_5206


5890863
C





G/C
cds_wt_5206


5890864
G





A/G
cds_wt_5206


5890869
G





T/G
cds_wt_5206


5890872
C





T/C
cds_wt_5206


5890893
C





T/C
cds_wt_5206


5890899
C





T/C
cds_wt_5206


5890906
G





A/G
cds_wt_5206


5890908
G





G/A
cds_wt_5206


5890914
C





T/C
cds_wt_5206


5890920
C





G/C
cds_wt_5206


5890923
C





T/C
cds_wt_5206


5890926
C





G/C
cds_wt_5206


5890971
C

C/T
C/T
C/T
C/T
C/T
cds_wt_5206


5890992
C
T/C
T/C
T/C
T/C
T/C
C/T
cds_wt_5206


5891058
G





C/G
cds_wt_5206


5891061
G





C/G
cds_wt_5206


5891070
C





T/C
cds_wt_5206


5891094
T





C/T
cds_wt_5206


5891097
C





C/G
cds_wt_5206


5891100
C





C/T
cds_wt_5206


5891121
C





T/C
cds_wt_5206


5891142
G





G/C
cds_wt_5206


5891145
C





C/G
cds_wt_5206


5891148
G





C/G
cds_wt_5206


5891187
G

G/C
G/C
G/C
G/C
G/C
cds_wt_5206


5891220
G





G/C
cds_wt_5206


5891247
T





T/C
cds_wt_5206


5891250
C





C/T
cds_wt_5206


5891263
T





T/C
cds_wt_5206


5892300
C





C/T
cds_wt_5206


5892304
A





A/C
cds_wt_5206


5892312
C





C/G
cds_wt_5206


5892330
C





C/T
cds_wt_5206


5892370
A





A/G
cds_wt_5206


5892413
C





C/T
cds_wt_5206


5892414
T





T/C
cds_wt_5206


5892456
A





A/T
cds_wt_5206


5892519
G
G/A
G/A
G/A
G/A
G/A
G/A
cds_wt_5206


5894264
G





A
cds_wt_5207


5894322
G





A
cds_wt_5207


5894986
A





A/G
cds_wt_5207


5894989
T





T/C
cds_wt_5207


5895041
G





G/A
cds_wt_5207


5895043
C





C/G
cds_wt_5207


5895049
C





C/G
cds_wt_5207


5895054
T





T/C
cds_wt_5207


5895058
G





G/C
cds_wt_5207


5895073
C
C/G
C/G
C/G
C/G
C/G
C/G
cds_wt_5207


5895100
G
G/C
G/C
G/C


G/C
cds_wt_5207


5895106
C





G/C
cds_wt_5207


5895108
T





C/T
cds_wt_5207


5895115
C





G/C
cds_wt_5207


5895118
A





C/A
cds_wt_5207


5895127
C





G/C
cds_wt_5207


5895130
C





G/C
cds_wt_5207


5895137
A





T/A
cds_wt_5207


5895142
T





C/T
cds_wt_5207


5895147
G





C/G
cds_wt_5207


5895181
C
C/T
C/T
C/T
C/T
C/T
T/C
cds_wt_5207


5895254
A





G/A
cds_wt_5207


5895280
T
T/C
C/T
T/C
T/C
T/C
C/T
cds_wt_5207


5895283
G
G/C
G/C
G/C
G/C
G/C
C/G
cds_wt_5207


5895298
C
C/G
C/G
C/G
C/G
C/G
G/C
cds_wt_5207


5895299
G
G/A
G/A
G/A
G/A
G/A
A/G
cds_wt_5207


5895319
C





G/C
cds_wt_5207


5895328
C





T/C
cds_wt_5207


5895334
C





T/C
cds_wt_5207


5895340
G





C/G
cds_wt_5207


5895346
G





G/C
cds_wt_5207


5895349
C





T/C
cds_wt_5207


5895358
C





T/C
cds_wt_5207


5895361
C





G/C
cds_wt_5207


5895370
C





C/T
cds_wt_5207


5895391
C





T/C
cds_wt_5207


5895436
G





C/G
cds_wt_5207


5895437
A





G/A
cds_wt_5207


5895439
C





G/C
cds_wt_5207


5895493
G

G/C
G/C
G/C
G/C
C/G
cds_wt_5207


5895496
G

G/C
G/C
G/C
G/C
C/G
cds_wt_5207


5895505
C





T/C
cds_wt_5207


5895529
T
T/C
C/T
T/C
T/C
T/C
C/T
cds_wt_5207


5895532
C
C/G
G/C
C/G
C/G
C/G
G/C
cds_wt_5207


5895577
G





C/G
cds_wt_5207


5895580
C





G/C
cds_wt_5207


5895628
T





C/T/G
cds_wt_5208


5895629
T





C/T
cds_wt_5208


5895661
C





C/G
cds_wt_5208


5895847
C
C/G
C/G
C/G
C/G
C/G
C/G
cds_wt_5208


5895878
G
G/A

G/A
G/A
G/A
G/A
cds_wt_5208


5895898
C

C/T
C/T
C/T
C/T
C/T
cds_wt_5208


5895899
A

A/C
A/C

A/C
A/C
cds_wt_5208


5895915
A
A/G
A/G
A/G
A/G
A/G
G/A
cds_wt_5208


5895949
C





T/C
cds_wt_5208


5896081
C
C/G
C/G
C/G
C/G
C/G
G/C
cds_wt_5208


5896085
C
C/T
C/T
C/T
C/T

T/C
cds_wt_5208


5896090
C
C/T
C/T
C/T
C/T

T/C
cds_wt_5208


5896115
C





C/T
cds_wt_5208


5896120
A





A/G
cds_wt_5208


5896123
G





G/C
cds_wt_5208


5896126
T





T/C
cds_wt_5208


5896147
T





T/G
cds_wt_5208


5896148
T





T/C
cds_wt_5208


5896154
T





T/C
cds_wt_5208


5896156
G





G/C
cds_wt_5208


5896159
G





G/C
cds_wt_5208


5896246
T





T/C
cds_wt_5208


5896279
C





T/C
cds_wt_5208


5896312
A





A/G
cds_wt_5208


5896324
A





A/G
cds_wt_5208


5896333
G





G/A
cds_wt_5208


5896381
G





G/C
cds_wt_5208


5900925
C





C/G
cds_wt_5209


5900943
G





G/T
cds_wt_5209


5900949
C





C/G
cds_wt_5209


5900955
C





C/T
cds_wt_5209


5900958
C





C/G
cds_wt_5209


5900965
T





T/C
cds_wt_5209


5900971
G





G/C
cds_wt_5209


5900972
C





C/G
cds_wt_5209


5900997
C





C/T
cds_wt_5209


5901000
C





C/T
cds_wt_5209


5901015
A





A/C
cds_wt_5209


5901034
C





C/A
cds_wt_5209


5901048
C





C/T
cds_wt_5209


5901050
G





G/A
cds_wt_5209


5901057
C





C/T
cds_wt_5209


5901068
G





G/A
cds_wt_5209


5901071
A





A/G
cds_wt_5209


5901078
G
G/T
G/T


G/T
G/T
cds_wt_5209


5901099
C
C/T
C/T
C/T
C/T
C/T
C/T
cds_wt_5209


5901123
T





T/A
cds_wt_5209


5901135
G





C/G
cds_wt_5209


5901172
G





A/G
cds_wt_5209


5901186
G





G/A
cds_wt_5209


5901195
T





T/C
cds_wt_5209


5901204
G





G/A
cds_wt_5209


5901219
C





A/C
cds_wt_5209


5901342
T
T/C
T/C
T/C
T/C
T/C
C/T
cds_wt_5209


5901345
T





T/G
cds_wt_5209


5901346
T





T/C
cds_wt_5209


5901354
C





C/G
cds_wt_5209


5901615
C
T/C
T/C
T/C
T/C
T/C
T/C
cds_wt_5209


5901617
G
C/G
C/G
C/G
C/G
C/G
C/G
cds_wt_5209


5901660
C





C/G
cds_wt_5209


5901664
T
T/G
T/G
T/G
T/G
T/G
T/G
cds_wt_5209


5901669
C
C/A
C/A

C/A
C/A
A/C
cds_wt_5209


5901673
T





A/T
cds_wt_5209


5901675
C





G/C
cds_wt_5209


5901679
C





C/T
cds_wt_5209


5901688
C





T/C
cds_wt_5209


5901689
T





A/T
cds_wt_5209


5901690
G





C/G
cds_wt_5209


5901698
C





A/C
cds_wt_5209


5901712
G





A/G
cds_wt_5209


5901713
T





A/T
cds_wt_5209


5901714
T





T/C
cds_wt_5209


5901719
G





G/C
cds_wt_5209


5901726
G





G/C
cds_wt_5209


5901729
C





C/G
cds_wt_5209


5901732
C





C/T
cds_wt_5209


5901733
G





G/A
cds_wt_5209


5901738
C





C/G
cds_wt_5209


5901752
C





C/T
cds_wt_5209


5901757
G





G/A
cds_wt_5209


5901764
T





C/T
cds_wt_5209


5901767
C





G/C
cds_wt_5209


5901771
G





C/G
cds_wt_5209


5901790
C





G/C
cds_wt_5209


5901791
G





C/G
cds_wt_5209


5901793
G





A/G
cds_wt_5209


5901807
G





C/G
cds_wt_5209


5901824
G





A/G
cds_wt_5209


5901826
G





G/A
cds_wt_5209


5901830
T





T/G
cds_wt_5209


5901840
A





A/C
cds_wt_5209


5902914
C
T
T
T
T
T
T
cds_wt_5209


5905708
G

A
A
A
A
A
cds_wt_5211


5925008
C
T
T
T
T
T
T
—


5925517
G

A
A
A
A
A
cds_wt_5227


5926357
G





A
cds_wt_5228


5928875
C




T
T
cds_wt_5231


5930205
C
T
T
T
T
T
T
cds_wt_5232


5933164
G
A
A
A
A
A
A
—


5947433
C
T
T
T
T
T
T
cds_wt_5246


5957471
G


A
A
A
A
—


5960825
C

T
T
T
T
T
cds_wt_5274


5961809
T
G/T


G/T
G/T
G/T
cds_wt_5275


5961866
T





T/A
—


5970914
C

T
T
T
T
T
cds_wt_5279


5975910
A
A/G
A/G
A/G
A/G
A/G
G/A
cds_wt_5283


5975911
A
A/G
A/G
A/G
A/G
A/G
G/A
—


5975926
A
A/G
A/G
A/G
A/G
A/G
G/A
—


5978267
C
T
T
T
T
T
T
cds_wt_5286


6005749
C

T
T
T
T
T
cds_wt_5311


6009383
G
A
A
A
A
A
A
cds_wt_5315


6010024
C


T
T
T
T
cds_wt_5316


6026234
C

T
T
T
T
T
—


6033950
C
T
T
T
T
T
T
cds_wt_5324


6042035
C





T
—


6050103
C




C/T
C/T
cds_wt_5343


6050112
A





C/A
cds_wt_5343


6050115
G





G/C
cds_wt_5343


6050116
C





C/G
cds_wt_5343


6050118
G





G/A
cds_wt_5343


6050509
G
G/A
A/G
A/G
A/G
A/G
A/G
cds_wt_5343


6050536
G





G/C
cds_wt_5343


6050556
G





C/G
cds_wt_5343


6050557
C





A/C
cds_wt_5343


6050584
T





T/A
cds_wt_5343


6050694
T





T/C
cds_wt_5343


6050727
A


G/A
G/A
G/A
G/A
cds_wt_5343


6050730
G

C/G
C/G
C/G
C/G
C/G
cds_wt_5343


6050769
G





G/A
cds_wt_5343


6050820
A





A/C
cds_wt_5343


6050822
T





T/C
cds_wt_5343


6050824
C





C/T
cds_wt_5343


6050947
T





C/T
cds_wt_5343


6050949
A





G/A
cds_wt_5343


6050952
T





T/C
cds_wt_5343


6050958
G





A/G
cds_wt_5343


6050965
A





C/A
cds_wt_5343


6050971
T





C/T
cds_wt_5343


6051079
A
G/A
G/A
G/A
G/A
G/A
A/G
cds_wt_5343


6051571
G
A
A
A
A
A
A
—


6072579
C



T
T
T
—


6074412
G
A
A
A
A
A
A
—


6075058
G
A
A
A
A
A
A
—


6083188
C




T
T
—


6085177
C




T
T
cds_wt_5373


6090298
C

T
T
T
T
T
cds_wt_5376


6094419
C
T
T
T
T
T
T
cds_wt_5383


6095825
C
T
T
T
T
T
T
cds_wt_5386


6098808
C
T
T
T
T
T
T
cds_wt_5388


6099142
C
T
T
T
T
T
T
cds_wt_5388


6099801
C
T
T
T
T
T
T
—


6100131
C
T
T
T
T
T
T
cds_wt_5389


6100928
C
T
T
T
T
T
T
cds_wt_5389


6114598
C

T
T
T
T
T
cds_wt_5399


6129757
G
A
A
A
A
A
A
—


6151020
G
A
A
A
A
A
A
cds_wt_5451


6157309
C

T
T
T
T
T
cds_wt_5456


6157376
C

T
T
T
T
T
cds_wt_5456


6178150
G

A
A
A
A
A
cds_wt_5472


6188180
C

T
T
T
T
T
cds_wt_5481


6191683
G
A


A

A
cds_wt_5487


6207945
G
A
A
A
A
A
A
cds_wt_5501


6216366
C


T
T

T
cds_wt_5511


6230439
A


G
G
G
G
cds_wt_5526


6251389
G

A
A
A
A
A
cds_wt_5542


6251652
G

A
A
A
A
A
cds_wt_5542


6255100
G

A
A
A
A
A
cds_wt_5546


6264179
G


A
A
A
A
cds_wt_5554


6265855
G

A
A
A
A
A
—


6267856
G


A
A

A
cds_wt_5556


6278700
G
A
A
A
A
A
A
—


6281103
C




T
T
cds_wt_5566


6282875
C

T
T
T
T
T
cds_wt_5567


6286620
C





T
cds_wt_5571


6288364
C


T
T
T
T
cds_wt_5571


6291716
C

T
T
T
T
T
cds_wt_5574


6297821
C

T
T
T
T
T
cds_wt_5578


6303632
G




A
A
cds_wt_5583


6306219
C

T
T
T
T
T
cds_wt_5586


6309112
G




A
A
cds_wt_5588


6322894
G





A
—


6328731
C
T
T
T
T
T
T
cds_wt_5605


6331035
G
A
A
A
A
A
A
cds_wt_5607


6338677
G
A
A
A
A
A
A
cds_wt_5615


6339429
C




T
T
—


6344180
C



T
T
T
cds_wt_5619


6346415
C



T

T
cds_wt_5622


6361477
C

T
T
T
T
T
cds_wt_5639


6362282
G


A
A
A
A
cds_wt_5639


6366940
C
T


T

T
cds_wt_5641


6368217
G




A
A
cds_wt_5643


6369699
G




A
A
cds_wt_5644


6375315
G
A
A
A
A
A
A
cds_wt_5648


6381431
C

T
T
T
T
T
cds_wt_5654


6401947
G
A
A


A
A
—


6403645
C



T
T
T
cds_wt_5675


6437676
C
T
T
T
T
T
T
cds_wt_5697


6439389
G




A
A
cds_wt_5698


6450624
C

T
T
T
T
T
—


6454248
C

T
T
T
T
T
cds_wt_5708


6454843
C




T
T
—


6456678
C




T
T
cds_wt_5711


6456912
C

T
T
T
T
T
cds_wt_5712


6459047
G





A
cds_wt_5715


6460584
T

C
C
C
C
C
—


6471884
G




A
A
cds_wt_5729


6485358
C

T
T
T
T
T
cds_wt_5741


6499172
G



A
A
A
cds_wt_5757


6500551
G





A
cds_wt_5760


6507389
C
T
T
T
T
T
T
cds_wt_5765


6517707
C


T
T

T
—


6525706
G




A
A
cds_wt_5775


6538378
C




T
T
cds_wt_5786


6542765
C

T
T
T
T
T
cds_wt_5790


6543445
C

T
T
T
T
T
cds_wt_5791


6543579
C

T
T
T
T
T
cds_wt_5791


6545779
C

T
T
T
T
T
cds_wt_5792


6550507
C




T
T
cds_wt_5797


6553219
G

A
A
A
A
A
cds_wt_5800


6553546
G


A


A
cds_wt_5800


6553589
G

A
A
A
A
A
cds_wt_5800


6555594
G

A
A
A
A
A
cds_wt_5804


6556192
C





T
cds_wt_5804


6560301
C

T
T
T
T
T
—


6561416
G

A
A
A
A
A
cds_wt_5810


6563658
C


T
T

T
cds_wt_5812


6564054
C

T
T
T
T
T
cds_wt_5812


6564511
C


T
T
T
T
cds_wt_5813


6564827
C

T
T
T
T
T
cds_wt_5813


6567102
C

T
T
T
T
T
cds_wt_5815


6567313
C




T
T
cds_wt_5816


6568360
C



T

T
cds_wt_5817


6569524
C

T
T
T
T
T
cds_wt_5819


6569696
G

A
A
A
A
A
cds_wt_5819


6570943
C

T
T
T
T
T
cds_wt_5820


6572188
C


T
T

T
cds_wt_5821


6572441
C


T
T

T
cds_wt_5821


6580904
G

A
A
A
A
A
cds_wt_5830


6581219
G





G/A
cds_wt_5830


6589934
C

T
T
T
T
T
cds_wt_5837


6595494
C
T

T
T

T
—


6604343
C





T
cds_wt_5851


6615272
C





T
cds_wt_5859


6617190
G
A
A
A
A
A
A
cds_wt_5861


6619058
C





T
cds_wt_5862


6622561
G
A
A
A
A
A
A
cds_wt_5864


6629950
C



T
T
T
cds_wt_5870


6632292
C
T
T
T
T
T
T
cds_wt_5872


6656015
C
T



T
T
cds_wt_5891


6669477
G




A
A
cds_wt_5901


6690831
C

T
T
T
T
T
cds_wt_5923


6690855
G




A
A
cds_wt_5923


6696960
G




A
A
—


6705369
G

A
A
A
A
A
cds_wt_5935


6716231
G

A
A
A
A
A
—


6729998
G

A
A
A
A
A
cds_wt_5955


6734341
C



T
T
T
cds_wt_5960


6758247
G




A
A
cds_wt_5984


6759384
C





T
cds_wt_5985


6767085
G




A
A
cds_wt_5990


6769984
C



T
T
T
cds_wt_5994


6780798
A


G
G
G
G
cds_wt_6005


6801699
G
A
A
A
A
A
A
cds_wt_6025


6805336
G
A
A
A
A
A
A
cds_wt_6026


6805990
G
A
A
A
A
A
A
cds_wt_6026


6808471
C

T
T
T
T
T
—


6818998
G

A
A
A
A
A
cds_wt_6038


6830990
G




A
A
cds_wt_6049


6843179
C





T
cds_wt_6061


6847192
G





A
cds_wt_6063


6854404
C





T
cds_wt_6068


6858684
G

A
A
A
A
A
cds_wt_6073


6883984
C

T
T
T
T
T
cds_wt_6092


6884717
G




A
A
cds_wt_6092


6890659
G

A
A
A
A
A
cds_wt_6098


6892977
G

A
A
A
A
A
cds_wt_6100


6898871
C

T
T
T
T
T
cds_wt_6107


6902839
A
T
T
T
T
T
T
cds_wt_6112


6911746
G

A
A
A
A
A
cds_wt_6117


6916561
G
A
A
A
A
A
A
cds_wt_6122


6916943
C

T
T
T
T
T
cds_wt_6122


6927456
G
A

A
A

A
cds_wt_6133


6960103
G


A
A
A
A
cds_wt_6158


6961070
C




T
T
cds_wt_6159


6968534
C
T
T
T
T
T
T
—


6973392
G

A
A
A
A
A
—


6987945
G

A
A
A
A
A
cds_wt_6176


6993597
G



A
A
A
cds_wt_6181


6995391
C

T
T
T
T
T
cds_wt_6182


6996621
C





T
cds_wt_6183


6999127
C

T
T
T
T
T
cds_wt_6187


6999520
C




T
T
cds_wt_6187


6999817
C

T
T
T
T
T
cds_wt_6188


7000264
C

T
T
T
T
T
cds_wt_6188


7002458
G

A
A
A
A
A
cds_wt_6190


7003486
G




A
A
cds_wt_6192


7003496
G




A
A
cds_wt_6192


7004068
C
T
T
T
T
T
T
cds_wt_6193


7009952
C




T
T
—


7010119
C




T
T
cds_wt_6198


7010712
C




T
T
cds_wt_6199


7015917
G

A
A
A
A
A
cds_wt_6203


7019067
C

T
T
T
T
T
—


7019437
C

T
T
T
T
T
cds_wt_6206


7020227
C


T
T
T
T
cds_wt_6207


7021026
C

T
T
T
T
T
cds_wt_6209


7023202
C

T
T
T
T
T
cds_wt_6212


7024113
C




T
T
cds_wt_6213


7026584
C

T
T
T
T
T
cds_wt_6217


7028273
C

T
T
T
T
T
cds_wt_6218


7028622
C




T
T
cds_wt_6218


7029812
C





T
cds_wt_6219


7031076
C





T
cds_wt_6219


7040546
C
T
T
T
T
T
T
cds_wt_6233


7053643
C

T
T
T
T
T
cds_wt_6243


7065316
C

T
T
T
T
T
cds_wt_6252


7075215
C

T
T
T
T
T
cds_wt_6264


7098642
C

T
T
T
T
T
cds_wt_6283


7099792
C

T
T
T
T
T
cds_wt_6284


7100617
C


T
T
T
T
cds_wt_6285


7101023
C

T
T
T
T
T
cds_wt_6285


7103392
C

T
T
T
T
T
—


7104098
C

T
T
T
T
T
cds_wt_6288


7109594
C

T
T
T
T
T
cds_wt_6291


7110600
C

T
T
T
T
T
cds_wt_6293


7110641
C

T
T
T
T
T
cds_wt_6293


7112833
C

T
T
T
T
T
cds_wt_6295


7116801
C
T
T
T
T
T
T
cds_wt_6297


7135624
G
A
A
A
A
A
A
cds_wt_6314


7135635
G
A




A
cds_wt_6314


7146220
G

A
A
A
A
A
—


7160415
C

T
T
T

T
—


7161529
G

A
A
A
A
A
cds_wt_6338


7162249
G

A
A
A
A
A
cds_wt_6338


7165660
C



T
T
T
—


7176091
G
A
A
A
A

A
cds_wt_6348


7177869
G
A
A
A
A
A
A
cds_wt_6350


7185280
G





A
cds_wt_6354


7186525
C

T
T
T
T
T
cds_wt_6355


7211965
G





G/C
cds_wt_6378


7211966
A





A/T
cds_wt_6378


7211975
C





C/T
cds_wt_6378


7211983
G





G/T
cds_wt_6378


7211992
G





G/T
cds_wt_6378


7211994
G





G/A
cds_wt_6378


7212006
T





T/C
cds_wt_6378


7212047
C





C/G
cds_wt_6378


7212067
T





T/A
cds_wt_6378


7212068
G





G/C
cds_wt_6378


7212075
G





G/C
cds_wt_6378


7212088
A





A/T
cds_wt_6378


7212097
T





T/C
cds_wt_6378


7212113
T





T/C
cds_wt_6378


7212121
G





G/T
cds_wt_6378


7212122
T





T/C
cds_wt_6378


7212142
T





T/C
cds_wt_6378


7212144
C





C/G
cds_wt_6378


7212151
T





T/G
cds_wt_6378


7212156
G





G/C
cds_wt_6378


7212180
C





C/G
cds_wt_6378


7212182
G





G/C
cds_wt_6378


7212183
T





T/C
cds_wt_6378


7212206
T





T/C
cds_wt_6378


7216330
G
A
A
A
A
A
A
cds_wt_6380


7218939
G
A
A
A
A
A
A
—


7219494
G




A
A
—


7224067
G





A
cds_wt_6388


7228023
G





A
—


7240925
G
A

A
A

A
cds_wt_6398


7241497
C


T
T

T
cds_wt_6399


7264121
G

A
A
A
A
A
cds_wt_6416


7266647
G
A
A
A
A
A
A
cds_wt_6419


7283171
C
T
T
T
T
T
T
cds_wt_6434


7294161
A

G
G
G
G
G
—


7303836
C
T
T
T
T
T
T
cds_wt_6454


7310019
G

A
A
A
A
A
cds_wt_6461


7314132
C

T
T
T
T
T
cds_wt_6463


7314146
C

T
T
T
T
T
cds_wt_6463


7319151
G
A
A
A
A
A
A
cds_wt_6467


7319658
G

A
A
A
A
A
cds_wt_6467


7321495
G





A
cds_wt_6470


7329181
G

A
A
A
A
A
cds_wt_6478


7330808
G

A
A
A
A
A
cds_wt_6480


7362245
C




T
T
cds_wt_6510


7369374
C




T
T
cds_wt_6518


7371536
C




T
T
cds_wt_6520


7377855
C

T
T
T
T
T
cds_wt_6527


7384932
G
A
A
A
A
A
A
cds_wt_6533


7385124
C

T
T
T
T
T
cds_wt_6533


7386113
G

A
A
A
A
A
cds_wt_6533


7389303
C

T
T
T
T
T
cds_wt_6535


7394213
C

T
T
T
T
T
cds_wt_6540


7394997
C




T
T
cds_wt_6540


7400636
C




T
T
—


7401592
C

T
T
T
T
T
cds_wt_6544


7422484
G

A
A
A
A
A
cds_wt_6563


7446288
G





A
—


7451085
C

T
T
T
T
T
—


7452627
C

T
T
T
T
T
cds_wt_6589


7453489
C
T
T
T
T
T
T
cds_wt_6590


7453910
C

T
T
T
T
T
cds_wt_6591


7460642
C

T
T
T
T
T
cds_wt_6600


7462223
G
A
A
A
A
A
A
cds_wt_6601


7484067
C
T
T
T
T
T
T
—


7490770
G




A
A
cds_wt_6634


7492384
G





A
cds_wt_6634


7498836
G

A
A
A
A
A
cds_wt_6639


7511194
G





A
cds_wt_6648


7517188
G

A
A
A
A
A
cds_wt_6653


7518982
C
T
T
T
T
T
T
cds_wt_6654


7524203
G

A
A
A
A
A
cds_wt_6659


7525798
C

T
T
T
T
T
cds_wt_6660


7546077
C

T
T
T
T
T
—


7583090
G


A
A
A
A
—


7588792
C

T
T
T
T
T
cds_wt_6730


7598819
G

A
A
A
A
A
—


7600514
G




A
A
cds_wt_6738


7604996
G





A
cds_wt_6743


7607903
G

A
A
A
A
A
cds_wt_6746


7622689
G

A
A
A
A
A
cds_wt_6763


7627683
G





G/T
cds_wt_6770


7630530
G
A
A
A
A
A
A
cds_wt_6772


7639840
G




A
A
cds_wt_6784


7641043
G





A
cds_wt_6785


7646004
G
A
A
A
A
A
A
cds_wt_6788


7648704
G
A
A
A
A
A
A
cds_wt_6791


7652691
G
A
A
A
A
A
A
cds_wt_6794


7654254
G
A
A
A
A
A
A
—


7655513
G
A
A
A
A
A
A
—


7662521
G

A

A

A
—


7671626
C



T
T
T
cds_wt_6808


7672126
G





A
cds_wt_6808


7687061
G





A
cds_wt_6826


7689723
G

A
A
A
A
A
cds_wt_6829


7714246
C

T
T
T
T
T
cds_wt_6856


7718521
G

A
A
A
A
A
cds_wt_6860


7719226
G

A
A
A
A
A
cds_wt_6861


7719430
G

A
A
A
A
A
cds_wt_6861


7720998
G

A
A
A
A
A
cds_wt_6862


7731353
G

A
A
A
A
A
cds_wt_6874


7733376
G




A
A
cds_wt_6876


7736672
C





C/T
cds_wt_6879


7740086
C
T
T
T
T
T
T
cds_wt_6883


7740896
G



A
A
A
cds_wt_6884


7765137
G

A
A
A
A
A
cds_wt_6902


7772239
G

A
A
A
A
A
cds_wt_6912


7789263
G
A
A
A
A
A
A
cds_wt_6933


7790304
G





A
cds_wt_6933


7801751
G

A
A
A
A
A
cds_wt_6947


7802623
T





T/C
—


7802638
T





T/C
—


7802639
C





C/T
—


7802682
A

T
T
T
T
T
—


7809752
G




A
A
cds_wt_6953


7809834
G




A
A
cds_wt_6953


7823754
C
T
T
T
T
T
T
cds_wt_6968


7825882
G



A
A
A
cds_wt_6969


7832540
G
A
A
A
A
A
A
cds_wt_6976


7838339
C

T
T
T
T
T
cds_wt_6980


7841392
C

T
T
T
T
T
cds_wt_6983


7841997
C

T
T
T
T
T
cds_wt_6983


7842274
C

T
T
T
T
T
cds_wt_6984


7844241
G

A
A
A
A
A
cds_wt_6985


7855301
C

T
T
T
T
T
cds_wt_6993


7864327
G
A
A
A
A
A
A
—


7874843
C





T
cds_wt_7008


7883195
A





G
cds_wt_7018


7889441
G

A
A
A
A
A
cds_wt_7022


7909245
G
A
A
A
A
A
A
cds_wt_7036


7912017
A





G
cds_wt_7038


7917279
G

A
A
A
A
A
cds_wt_7040


7926998
G
A

A
A
A
A
cds_wt_7045


7931764
G

A
A
A
A
A
cds_wt_7049


7934819
G

A
A
A
A
A
cds_wt_7051


7948630
C
T
T
T
T
T
T
cds_wt_7068


7963950
G


A
A
A
A
cds_wt_7082


7967045
G


A
A
A
A
cds_wt_7086


7969374
G
A
A
A
A
A
A
cds_wt_7089


7984213
G

A
A
A
A
A
cds_wt_7101


7984254
G

A
A
A
A
A
cds_wt_7101


7985275
G
A

A


A
cds_wt_7103


8009877
C
T
T
T
T
T
T
cds_wt_7127


8026754
C
T




T
cds_wt_7145


8036245
C

T
T
T
T
T
cds_wt_7152


8042744
G



A
A
A
cds_wt_7156


8051051
G
A
A
A
A
A
A
cds_wt_7165


8053700
C





T
cds_wt_7169


8062905
A





T
—


8062911
C





T
—


8075410
C
T
T
T
T
T
T
cds_wt_7189


8077440
G

A
A
A
A
A
cds_wt_7191


8087405
G
A
A
A
A
A
A
cds_wt_7205


8094018
G

A
A
A
A
A
cds_wt_7211


8095401
G
A




A
cds_wt_7213


8096270
C
T




T
cds_wt_7214


8122172
G





A
cds_wt_7240


8123295
G

A
A
A
A
A
cds_wt_7241


8123721
G

A
A
A
A
A
cds_wt_7241


8136640
C

T
T
T
T
T
cds_wt_7254


8161493
C

T
T
T
T
T
cds_wt_7274


8165695
A





A/T
cds_wt_7279


8165703
A





A/G
cds_wt_7279


8165704
C





C/G
cds_wt_7279


8167086
G

A
A
A
A
A
cds_wt_7280


8171741
G
A
A
A
A
A
A
cds_wt_7283


8179169
G



A
A
A
cds_wt_7288


8181933
G
A
A
A
A
A
A
cds_wt_7290


8186618
C
T
T
T
T
T
T
cds_wt_7297


8193241
C


T
T
T
T
cds_wt_7306


8195170
C

T
T
T
T
T
cds_wt_7307


8195310
C

T
T
T
T
T
cds_wt_7307


8199221
G
A
A
A
A
A
A
cds_wt_7312


8202704
C





C/T
cds_wt_7315


8205853
G
A
A
A
A
A
A
cds_wt_7319


8212158
C
T
T
T
T
T
T
cds_wt_7324


8212583
G




A
A
cds_wt_7324


8214951
C

T
T
T
T
T
cds_wt_7328


8221459
G
A
A
A
A
A
A
cds_wt_7335


8227017
G





A
cds_wt_7340


8233448
C




T
T
cds_wt_7347


8237580
G





A
cds_wt_7349


8239310
G





A
cds_wt_7349


8240439
G




A
A
cds_wt_7350


8241289
G

A
A
A
A
A
cds_wt_7350


8243590
G
A
A
A
A
A
A
—


8247542
G

A
A
A
A
A
cds_wt_7357


8248207
C





T
cds_wt_7357


8256006
G

A
A
A
A
A
cds_wt_7367


8263792
G

A
A
A
A
A
cds_wt_7373


8267543
G

A
A
A
A
A
cds_wt_7377


8272330
G





A
cds_wt_7379


8273351
C

T
T
T
T
T
cds_wt_7380


8278809
G



A
A
A
cds_wt_7384


8282707
G





A
cds_wt_7387


8298405
T
T/A
T/A

T/A

T/A
cds_wt_7401


8298413
C
C/G

C/G
C/G
C/G
C/G
cds_wt_7401


8302281
G
A
A
A
A
A
A
cds_wt_7405


8311927
T

C
C
C
C
C
cds_wt_7413


8323622
C

T
T
T
T
T
cds_wt_7423


8325049
G





G/C
cds_wt_7424


8325050
A





G/A
cds_wt_7424


8325073
C
C/G
C/G
C/G
C/G
C/G
C/G
cds_wt_7424


8325095
G





G/C
cds_wt_7424


8325096
G





G/T
cds_wt_7424


8325102
C





C/G
cds_wt_7424


8325109
T





C/T
cds_wt_7424


8325115
C





G/C
cds_wt_7424


8325118
C





C/G
cds_wt_7424


8325121
G





G/C
cds_wt_7424


8325127
G





G/C
cds_wt_7424


8325133
C





G/C
cds_wt_7424


8325203
T



T/C

T/C
cds_wt_7424


8325210
A
A/G
A/G
A/G
A/G
A/G
G/A
cds_wt_7424


8325277
C





C/G
cds_wt_7424


8325286
C





C/G
cds_wt_7424


8349257
G



A
A
A
cds_wt_7444


8351616
G



A
A
A
cds_wt_7445


8355565
C




T
T
cds_wt_7448


8355924
C

T
T
T
T
T
cds_wt_7448


8363036
G
A
A
A
A
A
A
cds_wt_7452a


8364757
C
T
T
T
T
T
T
cds_wt_7454


8365293
G
A
A
A
A
A
A
cds_wt_7454


8368404
G


A
A
A
A
cds_wt_7457


8378911
G

A
A
A
A
A
cds_wt_7464


8379977
G


A
A
A
A
—


8380006
C





T
—


8381094
G


A
A
A
A
cds_wt_7465


8384806
G



A
A
A
cds_wt_7468


8389000
C




T
T
cds_wt_7470


8389283
A
G
G
G
G
G
G
cds_wt_7470


8393915
G

A
A
A
A
A
cds_wt_7472


8396831
G





A
cds_wt_7476


8397563
G



A
A
A
cds_wt_7476


8402662
G

A
A
A
A
A
—


8411820
C


T
T
T
T
cds_wt_7490


8413318
C

T
T
T
T
T
cds_wt_7491


8414872
C

T
T
T
T
T
cds_wt_7491


8431655
T
A/T
A/T
A/T
A/T
A/T
T/A
—


8441210
C
T


T
T
T
cds_wt_7510


8449495
G





G/A
cds_wt_7513


8453858
G



A

A
cds_wt_7514


8463714
G




A
A
cds_wt_7525


8466767
G

A
A
A
A
A
cds_wt_7529


8491536
G
A
A
A
A
A
A
—


8497636
C





T
cds_wt_7552


8506267
G


A
A
A
A
cds_wt_7561


8509227
G





A
cds_wt_7562


8515828
G

A
A
A
A
A
cds_wt_7566


8529144
G

A
A
A
A
A
cds_wt_7572


8544731
G

A
A
A
A
A
—


8546526
C
T
T
T
T
T
T
cds_wt_7591


8556565
G

A



A
—


8559522
G
A
A
A
A
A
A
cds_wt_7602


8560241
C





T
cds_wt_7603


8567608
C

T
T
T
T
T
cds_wt_7607


8572238
C




T
T
—


8576774
G
A

A
A
A
A
—


8582623
C




T
T
cds_wt_7615


8589455
C




T
T
cds_wt_7621


8589459
C




T
T
cds_wt_7621


8595339
C
T
T
T
T
T
T
cds_wt_7627


8610015
C

T
T
T
T
T
cds_wt_7640


8622039
C
T
T
T
T
T
T
cds_wt_7652


8631175
G
A
A
A
A
A
A
cds_wt_7660


8640526
C

T
T
T
T
T
cds_wt_7667


8646416
G

A
A
A
A
A
cds_wt_7674


8647802
G

A
A
A
A
A
cds_wt_7675


8648408
G



A
A
A
cds_wt_7676


8655581
C
T
T
T
T
T
T
cds_wt_7683


8658688
G

A
A
A
A
A
cds_wt_7685


8668191
C

T
T
T
T
T
cds_wt_7693


8668196
G

A
A
A
A
A
cds_wt_7693


8669919
G
A
A
A
A
A
A
—


8674137
C
T
T
T
T
T
T
cds_wt_7700


8678961
G

A
A
A
A
A
cds_wt_7704


8682722
G
A
A
A
A
A
A
—


8690364
C
T
T
T
T
T
T
cds_wt_7711


8691972
C





T
cds_wt_7712


8699652
G

A
A
A
A
A
cds_wt_7716


8699708
G

A
A
A
A
A
cds_wt_7716


8709561
C

T
T
T
T
T
—


8720459
G
A
A
A
A
A
A
cds_wt_7731


8731485
G




A
A
cds_wt_7745


8737851
G
A
A
A
A
A
A
cds_wt_7749


8737859
G
A
A
A
A
A
A
cds_wt_7749


8742265
C

T

T
T
T
cds_wt_7750


8743148
C


T
T

T
cds_wt_7751


8754468
G

A
A
A
A
A
—


8754501
G

A
A
A
A
A
—


8759529
G





A
cds_wt_7757


8761290
C

T
T
T
T
T
cds_wt_7757


8769464
T




C
C
—


8792629
G

A
A
A
A
A
cds_wt_7788


8793001
C
T
T
T
T
T
T
cds_wt_7788


8809351
C

T
T
T
T
T
cds_wt_7801


8813761
C

T
T
T
T
T
cds_wt_7803


8813866
C

T
T
T
T
T
cds_wt_7803


8816396
G
A
A
A
A
A
A
cds_wt_7805


8821923
G

A
A
A
A
A
cds_wt_7810


8825490
G

A
A
A
A
A
cds_wt_7813


8828064
C

T
T
T
T
T
cds_wt_7815


8841828
G
A
A
A
A
A
A
cds_wt_7827


8842797
G




A
A
cds_wt_7828


8843541
G





A
cds_wt_7828


8845199
T

C
C
C
C
C
cds_wt_7829


8848419
G
A
A
A
A
A
A
cds_wt_7832


8849052
G
A
A
A
A
A
A
cds_wt_7833


8854017
C


T
T
T
T
cds_wt_7839


8854256
G

A
A
A
A
A
cds_wt_7839


8858030
G

A
A
A
A
A
cds_wt_7843


8859328
G





A
cds_wt_7844


8860995
G


A


A
cds_wt_7846


8861382
G

A
A
A
A
A
—


8871894
C
T
T
T
T
T
T
—


8872344
C





C/T
cds_wt_7854


8875526
G

A
A
A
A
A
cds_wt_7858


8881555
G



A
A
A
cds_wt_7864


8889242
G





A
cds_wt_7871


8893686
G
A
A
A
A
A
A
cds_wt_7877


8894581
G
A
A
A
A
A
A
—


8896018
G
A
A
A
A
A
A
cds_wt_7879


8901982
G
A
A
A
A
A
A
cds_wt_7883


8902511
G

A
A
A
A
A
cds_wt_7883


8902844
G

A
A
A
A
A
cds_wt_7884


8902903
G

A
A
A
A
A
cds_wt_7884


8907784
G
A
A
A
A
A
A
—


8927137
G





A
—


8929703
G
A
A
A
A
A
A
cds_wt_7911


8931762
C
T
T
T
T
T
T
cds_wt_7914


8934108
G




A
A
cds_wt_7916


8937518
C





T
cds_wt_7919


8943513
C





T
cds_wt_7925


8943676
C





T
cds_wt_7925


8944024
C





T
—


8945415
C


T
T
T
T
—


8946251
C


T
T
T
T
cds_wt_7927


8948806
G

A
A
A
A
A
cds_wt_7930


8950622
C
T
T
T
T
T
T
cds_wt_7931


8951908
C

T
T
T
T
T
cds_wt_7932


8953417
G
A

A
A

A
cds_wt_7935


8955117
G




A
A
cds_wt_7936


8956021
C





T
cds_wt_7937


8956608
C





T
cds_wt_7937


8958843
C
T


T
T
T
cds_wt_7939


8959688
G


A
A
A
A
cds_wt_7940


8974326
G

A
A
A
A
A
cds_wt_7953


8979884
C


T
T
T
T
cds_wt_7957


8982938
G
G/C
G/C



G/C
cds_wt_7963


8982956
G
G/C
G/C
G/C
G/C
G/C
G/C
cds_wt_7963


8982975
G





G/C
cds_wt_7963


8983002
G
G/A
G/A
G/A
G/A
G/A
G/A
—


8983053
C
C/T
C/T
C/T
C/T
C/T
C/T
cds_wt_7964


8983087
G





A/G
cds_wt_7964


8983088
G





G/C
cds_wt_7964


8983102
A
A/G
A/G
A/G
A/G
A/G
G/A
cds_wt_7964


8983134
G
G/C
G/C
G/C
G/C
G/C
G/C
cds_wt_7964


9008254
C





T
cds_wt_7977


9028349
G
A
A
A
A
A
A
cds_wt_7991


9029056
C




T
T
cds_wt_7991


9045846
G

A
A
A
A
A
cds_wt_7996


9049564
C





T
cds_wt_7998


9052240
C
T
T
T
T
T
T
cds_wt_8000


9052265
G


A
A
A
A
cds_wt_8000


9053107
G
A
A
A
A
A
A
—


9058239
G
A
A
A
A
A
A
cds_wt_8006


9060181
C

T
T
T
T
T
cds_wt_8008


9064090
C
T
T
T
T
T
T
cds_wt_8011


9072129
C





C/T
cds_wt_8017









Summary of Identified COG Categories

Table 10 shows details on COG-classified genes of the A. utahensis wild type strain SE50-100. For each COG category and its subcategories, the number of annotated genes and the absolute percentage is listed.











COG-Categories
Population

















CDS with COG-category
3,983
(49.62%)


 Information storage and processing
1240
(15.45%)


 Translation. ribosomal structure and biogenesis
200
(2.49%)


 RNA processing and modification
2
(0.02%)


 Transcription
771
(9.61%)


 Replication. recombination and repair
266
(3.31%)


 Chromatin structure and dynamics
1
(0.01%)


 Cellular processes and signaling
1398
(17.42%)


 Cell cycle control. cell division. chromosome
49
(0.61%)


partitioning




 Nuclear structure
0
(0.00%)


 Defense mechanisms
84
(1.05%)


 Signal transduction mechanisms
592
(7.38%)


 Cell wall/membrane/envelope biogenesis
302
(3.76%)


 Cell motility
104
(1.30%)


 Cytoskeleton
8
(0.10%)


 Extracellular structures
0
(0.00%)


 Intracellular trafficking. secretion. and vesicular
89
(1.11%)


transport




 Posttranslational modification. protein turnover.
170
(2.12%)


chaperones




 Metabolism
2409
(30.01%)


 Energy production and conversion
308
(3.84%)


 Carbohydrate transport and metabolism
575
(7.16%)


 Amino acid transport and metabolism
521
(6.49%)


 Nucleotide transport and metabolism
82
(1.02%)


 Coenzyme transport and metabolism
187
(2.33%)


 Lipid transport and metabolism
234
(2.92%)


 Inorganic ion transport and metabolism
257
(3.20%)


 Secondary metabolites biosynthesis. transport and
245
(3.05%)


catabolism









All Genes with Annotation
Table 11 lists all annotated genes of the Actinoplanes utahensis wild type strain SE50-100.








Lengthy table referenced here




US09719064-20170801-T00001


Please refer to the end of the specification for access instructions.














LENGTHY TABLES




The patent contains a lengthy table section. A copy of the table is available in electronic form from the USPTO web site (http://seqdata.uspto.gov/?pageRequest=docDetail&DocID=US09719064B2). An electronic copy of the table will also be available from the USPTO upon request and payment of the fee set forth in 37 CFR 1.19(b)(3).

## Claims

1. A mutated comprising DNA the nucleotide sequence of SEQ ID NO.: 16053 and the mutations shown in the Table below:












Position in
Nucleotide in
Mutated


SEQ ID 16053
SEQ ID 16053
Nucleotide

















7102
G
A


11919
G
A


12285
G
A


12651
G
A


13511
G
A


46974
C
T


62542
C
T


64957
C
T


65055
C
T


65455
C
T/C


65460
C
T


65882
G
A


72685
A
A/G


72693
A
A/G


72701
A
A/G


73373
C
T


73467
C
T


76845
C
T


78965
C
T


80653
G
A


85503
G
A


88269
G
A


88369
G
A


89368
C
T


89901
C
T


90029
C
T


91949
C
T


93427
C
T


101351
G
A


103967
C
T


104063
C
T


110857
C
T


112637
G
A


114499
G
A


115250
G
A


115873
C
T


115983
C
T


117494
C
T


119344
G
A


134489
C
T


139515
C
T


146767
C
T


161035
G
A


172303
G
A


176176
G
A


178880
G
G/A


180754
C
T


180925
C
T


184674
G
A


190568
C
T


190644
C
T


191971
C
T


193584
G
A


196028
C
T


196338
C
T


214992
G
A


222841
G
A


222924
C
T


228543
C
T


247200
C
T


248952
C
T


253299
G
A


254551
G
A


259829
C
T


268705
C
T


270822
G
A


273723
G
A


276430
C
T


276561
C
T


283007
G
A


283062
G
A


287132
C
T


294767
G
A


295356
C
T


300028
C
T


303810
G
A


313837
C
T


316698
G
A


316929
G
A


325503
C
T


331088
G
A


340040
G
A


342987
G
A


343915
G
A


352688
C
T


353668
G
A


361019
G
A


362742
G
A


375835
C
T


381358
G
A


382029
G
A


391677
C
T


400166
C
T


406413
G
A


409544
G
A


416199
G
A


419938
C
T


425067
G
A


425419
G
A


426464
G
A


438324
C
T


446368
G
A


446984
G
A


447392
G
A


450492
G
A


458678
C
T


459753
C
T


459833
A
G


466505
G
A


474923
G
A


482599
G
A


494993
G
A


500358
C
T


510408
C
T


511515
G
A


512816
G
A


513902
C
T


515197
G
A


518156
G
A


533618
G
A


536327
C
T


542891
G
A


548989
C
T


553081
G
A


553332
G
A


557884
G
A


557907
C
A


567026
C
T


587166
A
G


590721
G
A


602225
C
T


604184
C
T


605918
C
T


621774
C
T


624361
C
T


625600
C
T


626283
G
A


629283
G
A


636108
C
T


648981
G
A


684062
Ci
A


702171
C
T


702650
C
T


703452
C
T


706109
C
T


711168
G
A


712462
A
A/G


714678
G
A


714785
G
A


717602
G
A


719600
G
A


724097
G
A


738525
C
T


744594
G
A


746362
G
A


750285
C
T


752663
G
A


763762
C
C/G


763785
G
G/C


763792
C
C/G


763951
C
C/G


765635
G
A


766073
G
A


769289
G
A


776043
C
T


780677
C
T


781916
C
T


783900
C
T


786653
G
A


795572
C
T


796824
G
A


798663
G
A


810530
G
A


810533
C
G


810556
T
T/C


810557
G
G/C


810563
C
C/T


810576
A
A/G


819250
G
A


829153
C
T


830171
C
T


831404
G
A


838352
G
A


842068
G
A


847887
G
A


861775
C
T


870304
G
A


880820
C
T


897424
C
T


907842
G
A


908987
C
T


921289
G
A


941088
G
A


945666
G
A


959932
C
T


964504
G
A


964558
C
T


970334
C
T


972781
C
T


995285
G
A


1004653
C
T


1006503
C
T


1008680
G
A


1009674
G
A


1009813
G
A


1023301
C
T


1028963
G
A


1030345
C
T


1030822
G
A


1037262
G
A


1041811
C
T


1054013
C
T


1055406
C
T


1082623
G
A


1085482
C
T


1085499
C
T


1088995
C
T


1097530
C
T


1107807
T
A


1117905
C
T


1119169
G
A


1120103
G
A


1120852
G
A


1122832
C
T


1123080
C
T


1128153
G
A


1130585
G
A


1145784
C
T


1146897
G
A


1153166
C
T


1163219
C
T


1163309
G
A


1163538
G
A


1169629
C
T


1169882
C
T


1170591
C
T


1172808
C
T


1172940
C
T


1173551
C
T


1179238
C
A


1192548
C
T


1194346
C
T


1194637
G
A


1196403
C
T


1208932
C
T


1221399
C
T


1222202
C
T


1225212
C
T


1242677
G
A


1249095
C
T


1250477
G
A


1255855
C
T


1260719
C
T


1261347
C
T


1261926
C
T


1262307
G
A


1262367
G
A


1267545
C
T


1270664
C
T


1272928
C
T


1273797
C
T


1277491
G
A


1282245
G
A


1284346
C
T


1285883
C
T


1291340
C
T


1300654
G
A


1306705
C
T


1307526
C
T


1308200
C
T


1315876
C
T


1317961
G
A


1321981
C
T


1322304
C
T


1325473
C
T


1325983
G
A


1327837
C
T


1329364
C
T


1330802
G
A


1335338
C
T


1342390
C
T


1343472
A
C


1344261
C
T


1346995
C
T


1348395
G
A


1351344
C
T


1352745
C
T


1356389
C
T


1359105
G
A


1359182
C
T


1362032
C
T


1370065
G
A


1370088
C
T


1379821
C
T


1382638
G
A


1388800
C
T


1404278
C
T


1409508
G
A


1410045
G
A


1413757
G
A


1415711
G
A


1415773
G
A


1418132
C
T


1419671
G
A


1420016
C
T


1428228
C
T


1428233
C
T


1435884
C
T


1438355
C
T


1440872
G
A


1443149
C
T


1450678
G
A


1453590
G
A


1454075
C
T


1455191
G
A


1463521
C
T


1473239
C
T


1474775
G
A


1482297
C
T


1494980
G
A


1502729
G
A


1514463
C
T


1525258
G
A


1533361
C
T


1545597
T
T/C


1563822
C
T


1563854
C
T


1565818
C
T


1566866
C
T


1570236
G
A


1570636
C
T


1581033
T
C


1594972
G
A


1614616
C
T


1615586
C
T


1616152
C
T


1616981
C
T


1617561
C
T


1617928
C
T


1622589
G
A


1641656
C
T


1641711
C
T


1641953
C
T


1648653
G
A


1648676
G
A


1649444
C
T


1650400
G
A


1658589
G
A


1661376
G
A


1664324
C
T


1680524
C
T


1682349
G
A


1682842
C
T


1686608
C
T


1695571
C
T


1699167
G
A


1700705
G
A


1715788
T
C


1727072
G
A


1747963
C
T


1748448
C
T


1778278
C
T


1779396
C
T


1779723
C
T


1792608
G
A


1793069
C
T


1795610
C
T


1803172
C
T


1805902
C
T


1815104
C
T


1848170
G
A


1858054
C
T


1874864
G
A


1878368
C
T


1878456
C
T


1881797
G
A


1881821
G
A


1883780
G
A


1886024
G
A


1886177
G
A


1886499
G
A


1888003
G
A


1888277
G
A


1891012
G
A


1891205
C
T


1891493
G
A


1891608
G
A


1895230
C
T


1896121
G
A


1898465
G
A


1904126
G
A


1904415
G
A


1918600
G
A


1926767
G
A


1930239
G
A


1933689
G
A


1935111
G
A


1938524
G
A


1941158
G
A


1950155
C
T


1961896
C
T


1964247
C
T


1971635
A
C


1973884
G
A


1985968
G
A


1986215
C
T


1987891
C
T


1987907
C
T


1993612
G
A


1997079
C
T


2043866
G
A


2043889
G
A


2048938
C
T


2058063
G
A


2058998
G
A


2060664
G
A


2060796
G
A


2067415
G
A


2067911
G
A


2071535
G
A


2077305
A
G/A


2077309
T
C/T


2077321
A
G/A


2077324
G
A/G


2077336
A
G/A


2077351
C
T/C


2077353
A
G/A


2077369
C
T/C


2077383
G
A/G


2077384
C
G/C


2077398
G
G/C


2077432
T
C/T


2077603
A
G/A


2077615
C
A/C


2077618
G
A/G


2077636
T
C/T


2077645
G
C/G


2077651
G
C/G


2077652
A
G/A


2077654
A
G/A


2077663
C
A/C


2077669
G
C/G


2077671
T
C/T


2077682
C
G/C


2077688
G
A/G


2077691
C
G/C


2077694
A
C/A


2077697
G
C/G


2077703
T
G/T


2077715
G
A/G


2077724
A
G/A


2077726
C
T/C


2077730
G
C/G


2077733
G
A/G


2077736
C
C/T


2077742
G
C/G


2077745
A
G/A


2077751
G
A/G


2077762
T
C/T


2077769
T
C/T


2077772
A
G/A


2077775
G
A/G


2077793
G
C/G


2077794
A
G/A


2077795
C
T/C


2077805
C
T/C


2080486
C
T


2086608
G
A


2096915
G
A


2098836
C
T


2099830
G
A


2126265
C
T


2131730
G
A


2135109
C
T


2136848
C
T


2144528
G
A


2147486
G
A


2154132
C
T


2154136
C
T


2188490
C
T


2191494
C
T


2199567
G
A


2208975
G
A


2212324
G
A


2212659
G
A


2230643
G
A


2235434
G
A


2250499
C
T


2259118
C
T


2260582
G
A


2266821
C
T


2271223
G
A


2273696
C
T


2277990
C
T


2291798
C
T


2293101
C
C/T


2293102
G
G/T


2293121
G
G/C


2293139
A
A/C


2293157
G
G/A


2293169
G
G/C


2293175
G
G/A


2293176
A
A/T


2293184
C
C/G


2293185
G
G/A


2293187
A
A/G


2293196
G
A/G


2293202
G
G/C


2293205
G
C/G


2293214
G
G/C


2293219
A
A/T


2293220
G
G/C


2293226
G
C/G


2293228
G
G/A


2293234
A
A/T


2293235
G
G/C


2293238
G
G/C


2293244
G
G/C


2293247
G
A/G


2293253
G
A/G


2293268
G
C/G


2293310
G
G/A


2293313
G
G/C


2293316
G
G/A


2293319
G
G/A


2293328
G
G/C


2293337
G
G/C


2293346
G
G/C


2293352
C
C/G


2293367
G
G/C


2293376
C
C/G


2293403
C
C/G


2293463
C
C/G


2302130
G
A


2312744
C
T


2313183
G
A


2315988
C
T


2323085
C
T


2329242
C
T


2332081
G
A


2341627
C
T


2347673
C
T


2348681
G
A


2359598
G
A


2362060
A
G


2368326
G
A


2376154
G
A


2380176
C
T


2382542
C
T


2382567
C
T


2382748
C
T


2389975
C
T


2392147
C
T


2396255
G
A


2415101
G
A


2422620
C
T


2437185
G
A


2448470
G
A


2452760
G
A


2456608
C
T


2459347
C
T


2459641
G
A


2460824
C
T


2463427
C
T


2482048
C
T


2482381
G
A


2483453
C
T


2487377
G
A


2489641
G
A


2492054
G
A


2502775
G
A


2515457
G
A


2518872
G
A


2530466
G
A


2532233
G
A


2533237
G
A


2536306
G
A


2538874
G
A


2542087
G
A


2544720
C
T


2553277
G
A


2556662
C
T


2559280
G
A


2562212
G
A


2564253
G
A


2564620
G
A


2566353
G
A


2572994
G
A


2573153
C
T


2575327
G
A


2599645
G
A


2599894
G
A


2601319
G
A


2606340
G
A


2608948
G
A


2610422
C
T


2614778
G
A


2617082
G
A


2618776
C
T


2618805
C
T


2620558
G
A


2626414
A
G


2636037
C
T


2639682
G
A


2642753
G
A


2644984
C
T


2651718
C
T


2652733
C
T


2653074
C
T


2653159
C
T


2653337
G
A


2655271
C
T


2655308
C
T


2657059
C
T


2658261
G
A


2659295
C
T


2663775
C
T


2670944
C
T


2671043
C
T


2672551
C
T


2676880
C
T


2677056
G
A


2677196
C
T


2678066
G
A


2679900
C
T


2686364
C
T


2687028
C
T


2688632
C
T


2694405
G
A


2701281
G
A


2712327
G
A


2720850
C
T


2728435
G
A


2731397
G
A


2735550
G
G/A


2735576
G
G/C


2738768
C
T


2744600
C
T


2748694
C
T


2756873
C
T


2760595
G
A


2762887
G
A


2763137
G
A


2764279
C
T


2766645
C
T


2770562
C
T


2773173
C
T


2774095
G
A


2774734
G
A


2774874
G
A


2776314
C
T


2776919
C
T


2778598
G
A


2779539
G
A


2782696
C
T


2783578
C
T


2783738
C
T


2785372
C
T


2785531
C
T


2787379
G
A


2788015
C
T


2788247
C
T


2789216
C
T


2790626
C
T


2791577
C
T


2792510
C
T


2792715
G
A


2797814
C
T


2799953
C
T


2800033
C
T


2802765
C
T


2803789
C
T


2804694
C
T


2806670
C
T


2814611
G
A


2815199
C
C/T


2816239
G
A


2821693
G
A


2838356
C
T


2841171
T
T/A


2841177
C
C/G


2841183
C
C/G


2841213
T
T/C


2841228
C
C/G


2841237
G
G/C


2841251
G
G/T


2841255
C
C/A


2841258
G
G/T


2841276
C
C/G


2841293
C
C/G


2841297
C
C/A


2841303
C
C/T


2841306
G
G/A


2841312
T
C/T


2841315
C
G/C


2841322
A
A/T


2841323
G
G/T


2841327
C
G/C


2841335
G
C/G


2841357
G
C/G


2841360
G
C/G


2841369
G
C/G


2841376
T
T/C


2841377
G
T/G


2841384
G
C/G


2841389
T
T/C


2841397
G
G/T


2841398
A
C/A


2841402
G
A/G


2841403
G
C/G


2841409
T
A/T


2841413
G
T/G


2841423
C
G/C


2841444
G
C/G


2841451
C
T/C


2841477
C
G/C


2841514
G
T/G


2841531
A
G/A


2841534
G
C/G


2841536
T
A/T


2841540
C
G/G


2841546
G
C/G


2841552
G
C


2841554
T
C/T


2841555
C
T/C


2841562
C
T/C


2841563
A
C/A


2841569
A
C/A/T


2841575
C
T/C


2841585
C
C/G


2841588
G
C/G


2841594
G
A/G


2841605
C
C/A


2841609
C
C/G


2841617
T
G/T


2841636
G
C/G


2841656
A
C/A


2841660
G
C/G


2841668
C
T/C


2841690
G
C/G


2841700
G
T


2841701
C
T


2841708
G
A/G


2841734
T
C


2841735
C
T


2841738
G
C


2841744
C
G/C


2841747
G
A


2841756
C
G


2841759
G
C


2841762
C
G


2841776
G
T


2841778
T
G


2841783
G
C


2841786
A
T


2841789
C
T


2845157
G
A


2850318
G
A


2851372
G
A


2853353
C
T


2853817
G
A


2868405
C
T


2874011
C
T


2875055
G
A


2875488
G
A


2876911
G
T


2882407
C
T


2888545
C
T


2890510
C
T


2892791
G
A


2896239
G
A


2896828
G
A


2896833
G
A


2899845
C
T


2899865
C
T


2901123
C
T


2905469
C
T


2912026
G
A


2921100
C
T


2934976
G
A


2935134
G
A


2935223
G
A


2945715
G
A


2953167
C
T


2956464
C
T


2965167
G
A


2971031
C
T


2971761
C
T


2973653
C
T


2976296
C
T


2978371
C
T


2983351
G
A


2986416
C
T


2987675
C
T


2988967
C
T


2992035
C
T


2993614
A
G


2994097
G
A


3000921
G
A


3002748
G
A


3003382
G
A


3005352
G
A


3006201
C
T


3021497
G
A


3026718
C
T


3027132
G
A


3031683
C
T


3032969
C
T


3044082
G
A


3045579
G
A


3049857
G
A


3066905
C
T


3079916
T
A/T


3079923
C
C/T


3079936
G
A/G


3079950
A
T/A


3080004
C
C/T


3080006
A
T/A


3080035
C
C/T


3080043
A
C/A


3080063
G
C/G


3080084
C
C/T


3080087
G
C/T/G


3080098
C
C/A


3080128
C
T/C


3080136
A
G/A


3080160
C
G/C


3080161
T
C/T


3080163
T
C/T


3080183
T
C/T


3080192
G
C/G


3080204
G
C/G


3080237
G
C/G


3080288
G
C/G


3080294
G
T/G


3080316
C
C/T


3080321
T
T/C


3080325
G
T/G


3080326
C
T/C


3080355
A
T/A


3080356
A
A/G


3080357
A
A/G


3080359
A
A/G


3080372
A
A/G


3080375
A
A/C


3080382
G
G/A


3080391
A
A/G


3080393
C
C/T


3080394
G
G/A


3080402
A
A/G


3080406
A
A/G


3080418
A
A/G


3080447
C
C/T


3080465
T
T/C


3080472
C
C/T


3080482
A
A/G


3080485
C
C/T


3080496
T
T/C


3082062
G
A


3084014
G
A


3087970
C
T


3089578
G
A


3090231
G
G/C


3101153
G
A


3120006
C
T


3121040
C
T


3125035
C
T


3125407
C
T


3128098
C
T


3131738
C
T


3133651
C
T


3133766
G
A


3137673
C
T


3138173
G
T


3147694
C
T


3148142
C
T


3148342
C
T


3148452
C
T


3148777
C
T


3148960
C
T


3149335
C
T


3151547
C
T


3152279
C
T


3153365
G
A


3156844
C
T


3175067
G
A


3176311
C
T


3180510
C
T


3181134
C
T


3196810
C
T


3198674
G
A


3203788
G
A


3204764
G
T


3208464
G
T


3213080
C
T


3214059
C
T


3216019
C
T


3216133
C
T


3218109
C
T


3226281
C
T


3229241
G
A


3230387
G
A


3235932
C
T


3237109
C
T


3238700
C
T


3241279
C
T


3241643
G
A


3245145
C
T


3247705
G
A


3248186
G
A


3249319
C
T


3251559
G
A


3253347
C
T


3253453
C
T


3253884
G
A


3254464
G
A


3258028
G
A


3258094
G
A


3258637
C
T


3259667
C
T


3261792
G
A


3262978
C
T


3266668
G
A


3266806
C
T


3267341
G
A


3267927
C
T


3268140
T
C


3269676
G
A


3270548
G
A


3275254
A
G


3276870
C
T


3277187
C
T


3279932
C
T


3285105
C
T


3289175
C
T


3289533
C
T


3290417
G
A


3290880
C
T


3291408
C
T


3294618
C
T


3295292
C
T


3296150
C
T


3296689
C
T


3297254
C
T


3297593
G
A


3297802
C
T


3297816
G
A


3299219
C
T


3300457
C
T


3300838
C
T


3300849
C
T


3307410
G
A


3307738
G
A


3309205
C
T


3309441
G
A


3313410
C
T


3314734
C
T


3315483
C
T


3316995
C
T


3318906
C
T


3319964
C
T


3321895
G
A


3326784
G
A


3327191
G
A


3328753
C
T


3336319
C
T


3337206
G
A


3338688
C
T


3345311
C
T


3346757
G
A


3347355
C
T


3347540
C
T


3347560
C
T


3348860
C
T


3349015
C
T


3349651
C
T


3350820
C
T


3351613
C
T


3353030
C
T


3354271
C
T


3354637
C
T


3358411
G
G/A


3358883
G
A


3361525
G
A


3363657
C
T


3368167
C
T


3368381
C
C/T


3375301
C
T


3381507
C
T


3383846
C
T


3386322
C
T


3386914
G
A


3388650
C
T


3388663
C
T


3388840
G
A


3390202
G
A


3391187
C
T


3394129
C
T


3395838
C
T


3396692
C
T


3397717
C
T


3400152
C
T


3402866
G
A


3403044
C
T


3403253
C
T


3404959
C
T


3405078
C
T


3409620
G
A


3412544
G
A


3415574
A
G


3418558
C
T


3418906
G
A


3420004
C
T


3420345
C
T


3429994
C
T


3430020
G
A


3431269
C
T


3432683
G
A


3433788
G
A


3434662
C
T


3435439
G
A


3435661
G
A


3440357
G
A


3441404
C
T


3453288
C
T


3454769
G
A


3456480
C
T


3457161
C
T


3461043
C
T


3461116
G
A


3464902
C
T


3469479
C
T


3473066
G
A


3474749
C
T


3484337
G
A


3484696
C
T


3490633
G
A


3511395
C
T


3517523
G
A


3518338
C
T


3519210
C
T


3519280
C
T


3526212
G
A


3526229
G
A


3532172
C
T


3532281
C
T


3539856
C
T


3540438
C
T


3540952
C
T


3553678
G
A


3558780
G
A


3559503
G
A


3560298
T
C


3572036
C
T


3587529
C
T


3590513
C
T


3590937
C
T


3596971
C
T


3601304
C
T


3610733
G
A


3613460
C
T


3617806
G
A


3618063
C
T


3618082
G
A


3621113
G
A


3625554
C
T


3626449
C
T


3626548
C
T


3627377
C
T


3627686
C
T


3628977
C
T


3632788
C
T


3647003
C
T


3650159
G
A


3658514
C
T


3659174
C
T


3672218
G
A


3695232
G
A


3702963
C
T


3704185
C
T


3704278
G
G/C


3704281
T
T/G


3704282
C
C/T


3704293
T
T/G


3704327
G
A/G


3704335
C
T/C


3704338
C
G/C


3704340
A
C/A


3704371
G
C/G


3704386
T
C/T


3704389
C
G/C


3704395
G
C/G


3704404
G
C/G


3704415
C
T/C


3704427
G
A/G


3704428
C
T/C


3704464
C
T/C


3704484
A
C/A


3704485
C
G/C


3704491
G
C/G


3704512
G
C/G


3704519
A
G/A


3704531
A
GA


3704534
G
C/G


3704536
C
G/C


3704543
C
G/C


3704544
T
C/T


3704554
T
T/G


3704563
C
G/C


3704573
A
GA


3704574
A
G/A


3704577
G
C/G


3704584
C
G/C


3704587
G
A/G


3704590
G
C/G


3704605
T
C/T


3704608
C
G/C


3704617
T
G/T


3704619
A
C/A


3704620
C
A/C


3704629
C
G/C


3704638
C
G/C


3704641
G
C/G


3704647
C
G/C


3704650
G
C/A


3704660
C
A/C


3704662
G
A/G


3704668
T
C/T


3704669
G
A/G


3704670
T
C/T


3704680
C
G/C


3704684
C
G/C


3704686
G
C/G


3704746
G
C/G


3704755
C
C/G


3704767
A
A/G


3704782
T
T/C


3704789
A
VG


3704791
C
C/G


3704806
C
C/T


3704815
T
T/C


3704822
C
C/G


3704844
C
C/A


3704851
A
A/G


3704852
C
C/A


3704866
T
T/C


3704869
C
C/G


3704875
C
C/G


3704906
G
A


3706589
C
C/G


3706625
G
G/C


3706641
G
G/T


3706652
G
G/A


3706655
C
C/G


3706679
T
T/C


3706715
C
C/G


3706735
C
C/G


3706737
A
A/G


3706739
G
G/C


3706784
C
C/T


3706790
T
T/C


3706794
A
A/G


3706805
G
G/C


3706822
A
A/G


3706862
C
C/G


3706926
G
G/T


3706932
A
A/C


3706940
G
G/A


3706945
G
G/C


3706950
T
T/A


3706951
C
C/G


3706958
T
T/C


3706960
A
A/G


3706967
C
C/G


3706983
C
C/G


3706985
G
G/C


3706994
G
G/C


3706996
A
A/C


3707006
G
G/C


3707012
C
C/A


3707013
A
A/G


3707036
C
C/G


3711369
G
A


3713575
C
T


3724878
G
A


3724955
G
A


3727102
G
A


3727130
G
A


3727909
C
T


3732046
C
T


3733052
G
A


3740023
G
A


3741710
G
A


3744813
G
A


3750360
G
A


3756113
C
T


3762328
G
A


3763755
G
A


3764788
G
A


3771690
C
T


3775098
G
A


3775718
G
A


3776484
G
A


3783703
T
C


3786947
G
A


3788243
C
T


3798429
G
A


3799202
G
A


3799222
G
A


3802171
C
T


3806580
C
T


3825163
C
T


3828141
G
A


3831054
G
A


3831114
G
A


3831589
C
T


3831701
G
A


3837175
G
A


3841387
G
A


3843222
C
T


3843509
G
A


3843815
G
A


3844238
G
A


3846121
G
A


3846452
G
A


3852914
T
C


3873931
G
A


3879758
G
A


3883035
C
T


3905636
C
T


3919216
C
T


3920191
G
A


3928659
C
T


3935704
C
C/T


3941486
G
A


3964297
G
A


3965972
G
A


3968608
C
T


3983198
C
T


3993744
G
G/A


4002370
G
A


4004770
C
T


4006580
C
T


4006932
G
A


4013932
G
A


4014337
T
C


4017638
C
T


4019106
G
A


4020770
C
T


4025302
C
T


4027304
G
A


4034139
G
G/T


4043320
A
G


4063459
G
A


4072648
C
T


4073198
C
T


4073446
G
A


4074524
C
T


4081747
C
T


4086210
A
G


4089001
C
T


4102977
C
T


4104004
C
T


4114649
G
A


4117981
G
A


4118234
C
T


4121337
G
A


4124406
G
A


4130087
C
T


4148633
C
T


4150599
C
T


4152842
C
T


4156366
G
A


4156524
G
A


4157742
Ci
A


4157795
Ci
A


4158022
G
A


4160265
G
A


4170648
C
T


4186914
C
T


4187188
G
A


4192330
C
T


4192395
C
T


4194086
C
T


4196729
C
T


4205330
C
T


4218066
G
A


4220842
G
A


4231722
C
T


4251577
C
T


4253562
G
A


4255089
G
A


4268915
C
T


4284264
C
T


4285070
G
A


4286615
G
A


4299670
C
T


4302785
G
A


4302812
C
T


4312528
G
A


4312875
C
T


4314885
G
A


4314896
C
T


4314899
G
A


4316350
C
T


4318874
C
T


4319625
C
T


4324089
G
A


4325174
G
A


4325955
G
A


4327176
C
T


4328372
C
T


4329685
C
T


4329867
C
T


4331851
C
T


4331916
C
T


4334253
C
T


4335755
C
T


4340706
C
T


4343282
T
C


4347901
C
T


4352755
G
A


4360959
G
A


4361293
C
T


4362371
C
T


4365880
G
A


4376161
C
T


4376238
C
T


4378853
G
A


4395837
G
A


4396016
C
T


4402736
C
T


4406151
G
A


4414580
G
A


4414632
C
T


4420655
C
T


4433146
G
A


4438071
G
A


4440571
T
C


4440772
G
A


4443633
G
A


4444731
C
T


4446487
T
C


4463567
C
T


4463766
G
A


4467838
G
A


4474494
G
A


4481185
G
A


4486135
C
T


4487769
G
A


4490385
G
A


4496083
G
A


4499876
C
T


4503563
C
T


4504062
C
T


4517204
G
A


4524639
G
A


4524706
G
A


4529241
G
A


4529778
G
A


4531772
G
A


4532547
G
A


4532943
G
A


4533824
G
A


4535251
G
A


4537480
G
A


4537859
G
A


4543587
G
A


4547764
C
T


4558815
C
T


4558914
C
T


4564648
G
A


4564991
G
A


4573497
C
T


4573536
C
T


4575408
C
T


4577334
C
T


4580403
G
A


4582642
T
C


4588387
C
A


4588663
G
A


4597434
C
T


4598262
C
T


4601247
G
G/C


4601248
T
T/C


4603166
C
T


4611131
C
T


4614475
C
T


4616665
G
A


4617801
G
A


4619989
C
T


4621439
G
A


4626601
G
A


4630752
C
T


4649811
A
A/G


4650590
C
T


4669551
C
T


4670815
G
A


4674426
G
A


4677968
C
T


4688341
C
T


4697092
C
T


4700003
G
A


4702254
C
T


4704691
C
T


4704704
C
T


4713174
C
T


4727776
G
A


4736024
C
T


4766839
C
T


4769655
C
T


4776927
G
A


4788381
C
T


4794373
C
T


4799012
G
A


4812357
C
T


4813704
G
G/T


4813710
G
G/T


4813711
A
A/C


4814060
C
C/T


4814078
C
C/T


4814081
C
C/T


4814082
G
G/C


4814087
T
T/C


4818340
G
A


4820651
C
T


4821863
G
A


4852073
T
T/C


4852080
T
C/T


4852093
T
C/T


4852131
G
A/G


4852238
T
C/T


4857271
T
T/C


4852348
G
G/A


4852373
T
T/C


4858842
C
T


4859921
G
A


4860949
G
A


4872907
G
A


4879205
C
T


4886810
C
T


4889101
G
A


4891956
C
T


4896797
C
T


4903124
G
A


4904325
A
A/G


4904344
C
C/G


4904345
C
C/T


4904355
A
G


4904359
G
G/C


4904360
T
T/C


4908341
C
T


4908687
C
T


4911304
C
T


4916472
C
T


4920831
C
T


4924034
C
T


4924484
C
T


4927068
C
T


4927798
C
T


4929941
C
T


4930693
C
T


4932671
C
T


4933447
T
T/C


4948234
G
A


4950870
C
T


4951932
C
T


4953821
C
T


4978515
G
A


4981742
C
T


4984320
A
G


4987021
G
A


4987606
G
A


4991441
G
A


4992870
G
A


5014441
C
T


5016187
C
T


5016773
C
T


5025119
G
A


5030757
C
T


5053257
C
T


5056010
C
T


5056241
C
T


5058845
C
T


5058850
C
T


5060436
C
T


5061245
C
T


5062621
C
T


5063656
C
T


5063753
A
A/G


5063759
T
T/G


5063766
C
C/T


5063772
A
A/G


5063775
T
T/C


5063777
A
A/G


5063883
T
T/C


5063900
G
G/A


5063967
T
C/T


5063994
C
C/G


5063997
T
C/T


5064000
G
C/G


5064054
C
C/G


5064140
T
A/T


5064188
G
G/C


5064199
G
A/G


5064223
T
C/T


5064318
G
G/A


5064363
G
G/A


5064364
C
C/G


5064368
T
C/T


5066806
C
T


5079654
C
T


5080712
C
T


5080760
G
A


5083716
C
T


5085149
C
T


5087053
C
T


5089288
G
A


5095356
C
T


5096131
C
T


5099106
C
T


5099331
C
T


5099501
C
T


5104316
C
T


5104896
C
T


5105840
C
T


5107273
C
T


5107438
G
A


5110964
G
A


5111341
G
A


5111525
G
A


5113971
C
T


5114926
C
T/C


5114933
C
T/C


5116995
G
A


5117622
G
A


5126463
G
A


5128522
G
A


5131328
G
A


5132598
C
T


5133033
C
T


5134996
G
A


5136119
C
T


5139533
C
T


5141709
G
A


5147647
C
T


5158997
C
T


5171223
G
A


5189948
C
T


5196838
C
T


5203530
G
A


5204859
C
T


5213315
G
A


5216015
G
A


5221083
C
T


5229965
G
A


5233924
G
A


5246591
G
A


5247914
C
T


5253451
C
T


5255901
C
T


5259871
G
A


5270798
G
A


5277822
G
A


5280936
C
T


5283902
G
A


5292603
C
T


5309793
G
A


5317263
G
A


5323245
G
A


5323289
C
T


5323708
G
A


5326705
G
A


5339380
G
A


5349635
C
T


5350111
G
A


5356868
C
T


5363828
C
T


5385564
C
T


5391456
G
A


5404805
G
A


5415953
G
A


5416207
C
T


5419045
C
T


5438747
G
A


5457995
G
A


5465469
C
T


5481386
G
A


5485106
C
T


5500846
G
A


5514802
C
T


5516464
C
T


5530664
C
T


5537010
G
A


5540526
G
A


5541233
C
T


5543634
C
T


5545010
C
T


5545574
G
A


5548499
C
T


5557711
G
A


5574966
C
T


5576016
C
T


5579654
C
T


5582215
C
C/T


5590781
T
T/A


5590796
C
C/T


5590809
T
T/C


5590810
G
G/A


5590823
G
G/A


5590828
A
A/G


5590847
G
G/T


5590861
G
G/C


5590867
A
A/C


5590868
A
A/G


5590886
G
A/G


5590889
T
C/T


5590903
A
G/A


5590909
G
T/G


5590910
C
G/C


5590912
G
T/G


5590918
G
A/G


5590923
A
G/A


5590927
T
G/T


5590933
G
A/G


5590945
C
G/C


5590960
T
C/T


5590961
C
T/C


5590972
T
C/T


5590978
T
C/T


5590981
G
C/G


5590987
T
C/T


5590993
C
G/C


5591002
G
G/A


5591008
T
C/T


5591026
C
G/C


5591035
C
G/C


5591054
C
A/C


5591063
T
C/T


5591065
C
G/C


5591069
A
A/G


5591074
A
G/A


5591077
C
A/C


5591092
A
G/A


5591095
G
C/G


5591098
T
C/T


5591115
G
A/G


5591158
G
T/G


5591179
G
T/G


5591220
C
G/C


5591226
A
G/A


5591268
T
C/T


5591361
C
G/C


5591442
G
A/G


5591445
C
T/C


5591457
G
C/G


5591458
A
C/A


5591466
G
C/G


5591469
G
A/G


5591472
C
G/C


5591476
A
A/G


5591496
G
C/G


5591499
A
C/A


5591505
G
C/G


5591508
T
G/T


5591520
A
G/A


5591532
C
T/C


5591541
C
T/C


5591553
G
A/G


5591562
G
T/G


5591565
G
C/G


5591571
A
G/A


5591577
A
C/A


5591596
C
T/C


5591598
G
C/G


5591607
C
T/C


5591613
T
C/T


5591632
C
A/C


5591635
G
G/T


5591640
T
T/C


5591643
C
C/T


5591654
A
A/C


5591656
A
A/G


5604643
G
A


5612009
C
T


5626626
C
T


5629121
C
T


5631426
C
T


5636810
G
A


5657449
C
T


5659036
G
A


5692125
C
T


5694507
C
T


5696833
C
T


5698190
C
T


5705110
C
T


5708625
C
T


5709728
C
T


5724677
C
T


5727195
C
T


5728510
C
T


5732980
C
T


5738712
G
A


5738846
C
T


5745077
C
T


5751489
C
T


5751716
C
T


5753910
C
T


5755351
C
T


5756752
C
T


5756803
C
T


5760058
C
G


5760301
C
T


5763377
C
T


5764363
C
T


5765100
C
T


5765446
C
T


5767623
C
T


5779664
C
T


5780052
C
T


5781808
C
T


5783477
G
A


5788208
C
T


5790337
C
T


5791404
C
T


5793414
G
A


5795059
C
T


5795465
G
A


5799803
G
A


5803395
C
T


5803716
G
A


5805398
G
A


5805735
G
A


5807160
C
T


5809295
G
A


5810587
G
T


5811908
C
T


5813504
C
T


5813556
C
T


5834083
C
T


5842153
G
C


5843698
T
T/C


5843724
C
C/A


5843727
C
C/T


5843751
G
G/A


5843752
G
G/C


5843766
A
A/G


5843783
A
A/G


5843786
G
G/C


5843787
C
C/G


5843795
G
G/C


5843796
T
T/A


5843797
G
G/T


5843804
C
C/G


5843810
C
C/T


5843818
T
T/C


5843819
T
T/G


5843831
C
C/T


5843863
G
G/T


5843878
C
C/T


5843881
G
G/T


5843888
G
G/A


5843890
G
G/C


5843897
C
C/G


5843899
G
G/T


5843902
C
C/T


5843942
C
C/T


5843965
G
C/G


5843980
C
C/G


5843981
C
C/T


5843987
C
C/G


5843988
C
C/T


5843992
C
C/T


5857131
C
T


5858663
C
T


5879695
G
A


5880188
T
T/C


5880203
G
G/C


5880223
C
C/T


5880241
A
A/C


5880306
C
C/T


5880307
T
T/C


5880349
A
A/T


5880412
G
G/A


5880421
C
C/T


5880430
A
A/G


5880517
T
T/C


5882826
G
A


5887657
G
C/G


5887660
G
C/G


5887684
T
C/T


5887693
T
C/T


5887696
G
C/G


5887718
G
G/A


5887732
G
G/C


5887734
G
G/A


5887765
A
A/G/C


5887771
G
G/C


5887774
G
G/A


5887775
C
C/T


5887784
T
T/C


5887792
T
T/C


5887810
G
G/C


5887813
G
G/C


5887816
G
G/C


5887820
G
G/C


5887824
C
C/G


5887831
G
G/C


5887837
C
C/T


5887843
C
C/G


5887892
G
A/G


5887931
G
G/A/C


5887937
G
G/C


5887938
C
C/G


5887942
C
C/G


5887945
G
G/C


5887951
G
C/G


5887960
G
G/C


5887969
G
G/C


5887970
C
C/G


5887971
A
A/G


5887974
G
G/T


5887975
C
C/T


5887977
T
T/A


5887982
C
C/A/T


5887988
G
G/T


5887990
C
C/G


5888003
A
A/C


5888004
A
A/G


5888005
A
A/G


5888026
C
C/T


5888050
G
G/C


5888053
G
G/C


5888056
G
G/C


5888083
G
G/C


5888092
C
C/G


5888095
C
C/G


5888102
G
G/A


5888103
T
T/C


5888122
C
C/T


5888143
G
G/C


5890782
G
G/C


5890839
T
C/T


5890849
T
A/T


5890851
C
G/C


5890863
C
G/C


5890864
G
A/G


5890869
G
T/G


5890872
C
T/C


5890893
C
T/C


5890899
C
T/C


5890906
G
A/G


5890908
G
G/A


5890914
C
T/C


5890920
C
G/C


5890923
C
T/C


5890926
C
G/C


5890971
C
C/T


5890992
C
C/T


5891058
G
C/G


5891061
G
C/G


5891070
C
T/C


5891094
T
C/T


5891097
C
C/G


5891100
C
C/T


5891121
C
T/C


5891142
G
G/C


5891145
C
C/G


5891148
G
C/G


5891187
G
G/C


5891220
G
G/C


5891247
T
T/C


5891250
C
C/T


5891263
T
T/C


5892300
C
C/T


5892304
A
A/C


5892312
C
C/G


5892330
C
C/T


5892370
A
A/G


5892413
C
C/T


5892414
T
T/C


5892456
A
A/T


5892519
G
G/A


5894264
G
A


5894322
G
A


5894986
A
A/G


5894989
T
T/C


5895041
G
G/A


5895043
C
C/G


5895049
C
C/G


5895054
T
T/C


5895058
G
G/C


5895073
C
C/G


5895100
G
G/C


5895106
C
G/C


5895108
T
C/T


5895115
C
G/C


5895118
A
C/A


5895127
C
G/C


5895130
C
G/C


5895137
A
T/A


5895142
T
C/T


5895147
G
C/G


5895181
C
T/C


5895254
A
G/A


5895280
T
C/T


5895283
G
C/G


5895298
C
G/C


5895299
G
A/G


5895319
C
G/C


5895328
C
T/C


5895334
C
T/C


5895340
G
C/G


5895346
G
G/C


5895349
C
T/C


5895358
C
T/C


5895361
C
G/C


5895370
C
C/T


5895391
C
T/C


5895436
G
C/G


5895437
A
G/A


5895439
C
G/C


5895493
G
C/G


5895496
G
C/G


5895505
C
T/C


5895529
T
C/T


5895532
C
G/C


5895577
G
C/G


5895580
C
G/C


5895628
T
C/T/G


5895629
T
CT


5895661
C
C/G


5895847
C
C/G


5895878
G
G/A


5895898
C
C/T


5895899
A
A/C


5895915
A
G/A


5895949
C
T/C


5896081
C
G/C


5896085
C
T/C


5896090
C
T/C


5896115
C
C/T


5896120
A
A/G


5896123
G
G/C


5896126
T
T/C


5896147
T
T/G


5896148
T
T/C


5896154
T
T/C


5896156
G
G/C


5896159
G
G/C


5896246
T
T/C


5896279
C
T/C


5896312
A
A/G


5896324
A
A/G


5896333
G
G/A


5896381
G
G/C


5900925
C
C/G


5900943
G
G/T


5900949
C
C/A


5900955
C
C/T


5900958
C
C/G


5900965
T
T/C


5900971
G
G/C


5900972
C
C/G


5900997
C
C/T


5901000
C
C/T


5901015
A
A/C


5901034
C
C/A


5901048
C
C/T


5901050
G
GA


5901057
C
C/T


5901068
G
G/A


5901071
A
A/G


5901078
G
G/T


5901099
C
C/T


5901123
T
T/A


5901135
G
C/G


5901172
G
A/G


5901186
G
G/A


5901195
T
T/C


5901204
G
G/A


5901219
C
A/C


5901342
T
C/T


5901345
T
T/G


5901346
T
T/C


5901354
C
C/G


5901615
C
T/C


5901617
G
C/G


5901660
C
C/G


5901664
T
T/G


5901669
C
A/C


5901673
T
A/T


5901675
C
G/C


5901679
C
C/T


5901688
C
T/C


5901689
T
A/T


5901690
G
C/G


5901698
C
A/C


5901712
G
A/G


5901713
T
A/T


5901714
T
T/C


5901719
G
G/C


5901726
G
G/C


5901729
C
C/G


5901732
C
C/T


5901733
G
G/A


5901738
C
C/G


5901752
C
C/T


5901757
G
G/A


5901764
T
C/T


5901767
C
G/C


5901771
G
C/G


5901790
C
G/C


5901791
G
C/G


5901793
G
A/G


5901807
G
C/G


5901824
G
A/G


5901826
G
GA


5901830
T
T/G


5901840
A
A/C


5902914
C
T


5905708
G
A


5925008
C
T


5925517
G
A


5926357
G
A


5928875
C
T


5930205
C
T


5933164
G
A


5947433
C
T


5957471
G
A


5960825
C
T


5961809
T
G/T


5961866
T
T/A


5970914
C
T


5975910
A
G/A


5975911
A
G/A


5975926
A
G/A


5978267
C
T


6005749
C
T


6009383
G
A


6010024
C
T


6026234
C
T


6033950
C
T


6042035
C
T


6050103
C
C/T


6050112
A
C/A


6050115
G
G/C


6050116
C
C/G


6050118
G
G/A


6050509
G
A/G


6050536
G
G/C


6050556
G
C/G


6050557
C
A/C


6050584
T
T/A


6050694
T
T/C


6050727
A
G/A


6050730
G
C/G


6050769
G
G/A


6050820
A
A/C


6050822
T
T/C


6050824
C
C/T


6050947
T
C/T


6050949
A
G/A


6050952
T
T/C


6050958
G
A/G


6050965
A
C/A


6050971
T
C/T


6051079
A
A/G


6051571
G
A


6072579
C
T


6074412
G
A


6075058
G
A


6083188
C
T


6085177
C
T


6090298
C
T


6094419
C
T


6095825
C
T


6098808
C
T


6099142
C
T


6099801
C
T


6100131
C
T


6100928
C
T


6114598
C
T


6129757
G
A


6151020
G
A


6157309
C
T


6157376
C
T


6178150
G
A


6188180
C
T


6191683
G
A


6207945
G
A


6216366
C
T


6230439
A
G


6251389
G
A


6251652
G
A


6255100
G
A


6264179
G
A


6265855
G
A


6267856
G
A


6278700
G
A


6281103
C
T


6282875
C
T


6286620
C
T


6288364
C
T


6291716
C
T


6297821
C
T


6303632
G
A


6306219
C
T


6309112
G
A


6322894
G
A


6328731
C
T


6331035
G
A


6338677
G
A


6339429
C
T


6344180
C
T


6346415
C
T


6361477
C
T


6362282
G
A


6366940
C
T


6368217
G
A


6369699
G
A


6375315
G
A


6381431
C
T


6401947
G
A


6403645
C
T


6437676
C
T


6439389
G
A


6450624
C
T


6454248
C
T


6454843
C
T


6456678
C
T


6456912
C
T


6459047
G
A


6460584
T
C


6471884
G
A


6485358
C
T


6499172
G
A


6500551
G
A


6507389
C
T


6517707
C
T


6525706
G
A


6538378
C
T


6542765
C
T


6543445
C
T


6543579
C
T


6545779
C
T


6550507
C
T


6553219
G
A


6553546
G
A


6553589
G
A


6555594
G
A


6556192
C
T


6560301
C
T


6561416
G
A


6563658
C
T


6564054
C
T


6564511
C
T


6564827
C
T


6567102
C
T


6567313
C
T


6568360
C
T


6569524
C
T


6569696
G
A


6570943
C
T


6572188
C
T


6572441
C
T


6580904
G
A


6581219
G
G/A


6589934
C
T


6595494
C
T


6604343
C
T


6615272
C
T


6617190
G
A


6619058
C
T


6622561
G
A


6629950
C
T


6632292
C
T


6656015
C
T


6669477
G
A


6690831
C
T


6690855
G
A


6696960
G
A


6705369
G
A


6716231
G
A


6729998
G
A


6734341
C
T


6758247
G
A


6759384
C
T


6767085
G
A


6769984
C
T


6780798
A
G


6801699
G
A


6805336
G
A


6805990
G
A


6808471
C
T


6818998
G
A


6830990
G
A


6843179
C
T


6847192
G
A


6854404
C
T


6858684
G
A


6883984
C
T


6884717
G
A


6890659
G
A


6892977
G
A


6898871
C
T


6902839
A
T


6911746
G
A


6916561
G
A


6916943
C
T


6927456
G
A


6960103
G
A


6961070
C
T


6968534
C
T


6973392
G
A


6987945
G
A


6993597
G
A


6995391
C
T


6996621
C
T


6999127
C
T


6999520
C
T


6999817
C
T


7000264
C
T


7002458
G
A


7003486
G
A


7003496
G
A


7004068
C
T


7009952
C
T


7010119
C
T


7010712
C
T


7015917
G
A


7019067
C
T


7019437
C
T


7020227
C
T


7021026
C
T


7023202
C
T


7024113
C
T


7026584
C
T


7028273
C
T


7028622
C
T


7029812
C
T


7031076
C
T


7040546
C
T


7053643
C
T


7065316
C
T


7075215
C
T


7098642
C
T


7099792
C
T


7100617
C
T


7101023
C
T


7103392
C
T


7104098
C
T


7109594
C
T


7110600
C
T


7110641
C
T


7112833
C
T


7116801
C
T


7135624
G
A


7135635
G
A


7146220
G
A


7160415
C
T


7161529
G
A


7162249
G
A


7165660
C
T


7176091
G
A


7177869
G
A


7185280
G
A


7186525
C
T


7211965
G
G/C


7211966
A
A/T


7211975
C
C/T


7211983
G
G/T


7211992
G
G/T


7211994
G
G/A


7212006
T
T/C


7212047
C
C/G


7212067
T
T/A


7212068
G
G/C


7212075
G
G/C


7212088
A
A/T


7212097
T
T/C


7212113
T
T/C


7212121
G
G/T


7212122
T
T/C


7212142
T
T/C


7212144
C
C/G


7212151
T
T/G


7212156
G
G/C


7212180
C
C/G


7212182
G
G/C


7212183
T
T/C


7212206
T
T/C


7216330
G
A


7218939
G
A


7219494
G
A


7224067
G
A


7228023
G
A


7240925
G
A


7241497
C
T


7264121
G
A


7266647
G
A


7283171
C
T


7294161
A
G


7303836
C
T


7310019
G
A


7314132
C
T


7314146
C
T


7319151
G
A


7319658
G
A


7321495
G
A


7329181
G
A


7330808
G
A


7362245
C
T


7369374
C
T


7371536
C
T


7377855
C
T


7384932
G
A


7385124
C
T


7386113
G
A


7389303
C
T


7394213
C
T


7394997
C
T


7400636
C
T


7401592
C
T


7422484
G
A


7446288
G
A


7451085
C
T


7452627
C
T


7453489
C
T


7453910
C
T


7460642
C
T


7462223
G
A


7484067
C
T


7490770
G
A


7492384
G
A


7498836
G
A


7511194
G
A


7517188
G
A


7518982
C
T


7524203
G
A


7525798
C
T


7546077
C
T


7583090
G
A


7588792
C
T


7598819
G
A


7600514
G
A


7604996
G
A


7607903
G
A


7622689
G
A


7627683
G
G/T


7630530
G
A


7639840
G
A


7641043
G
A


7646004
G
A


7648704
G
A


7652691
G
A


7654254
G
A


7655513
G
A


7662521
G
A


7671626
C
T


7672126
G
A


7687061
G
A


7689723
G
A


7714246
C
T


7718521
G
A


7719226
G
A


7719430
G
A


7720998
G
A


7731353
G
A


7733376
G
A


7736672
C
C/T


7740086
C
T


7740896
G
A


7765137
G
A


7772239
G
A


7789263
G
A


7790304
G
A


7801751
G
A


7802623
T
T/C


7802638
T
T/C


7802639
C
C/T


7802682
A
T


7809752
G
A


7809834
G
A


7823754
C
T


7825882
G
A


7832540
G
A


7838339
C
T


7841392
C
T


7841997
C
T


7842274
C
T


7844241
G
A


7855301
C
T


7864327
G
A


7874843
C
T


7883195
A
G


7889441
G
A


7909245
G
A


7912017
A
G


7917279
G
A


7926998
G
A


7931764
G
A


7934819
G
A


7948630
C
T


7963950
G
A


7967045
G
A


7969374
G
A


7984213
G
A


7984254
G
A


7985275
G
A


8009877
C
T


8026754
C
T


8036245
C
T


8042744
G
A


8051051
G
A


8053700
C
T


8062905
A
T


8062911
C
T


8075410
C
T


8077440
G
A


8087405
G
A


8094018
G
A


8095401
G
A


8096270
C
T


8122172
G
A


8123295
G
A


8123721
G
A


8136640
C
T


8161493
C
T


8165695
A
A/T


8165703
A
A/G


8165704
C
C/G


8167086
G
A


8171741
G
A


8179169
G
A


8181933
G
A


8186618
C
T


8193241
C
T


8195170
C
T


8195310
C
T


8199221
G
A


8202704
C
C/T


8205853
G
A


8212158
C
T


8212583
G
A


8214951
C
T


8221459
G
A


8227017
G
A


8233448
C
T


8237580
G
A


8239310
G
A


8240439
G
A


8241289
G
A


8243590
G
A


8247542
G
A


8248207
C
T


8256006
G
A


8263792
G
A


8267543
G
A


8272330
G
A


8273351
C
T


8278809
G
A


8282707
G
A


8298405
T
T/A


8298413
C
C/G


8302281
G
A


8311927
T
C


8323622
C
T


8325049
G
G/C


8325050
A
G/A


8325073
C
C/G


8325095
G
G/C


8325096
G
G/T


8325102
C
C/G


8325109
T
C/T


8325115
C
G/C


8325118
C
C/G


8325121
G
G/C


8325127
G
G/C


8325133
C
G/C


8325203
T
T/C


8325210
A
G/A


8325277
C
C/G


8325286
C
C/G


8349257
G
A


8351616
G
A


8355565
C
T


8355924
C
T


8363036
G
A


8364757
C
T


8365293
G
A


8368404
G
A


8378911
G
A


8379977
G
A


8380006
C
T


8381094
G
A


8384806
G
A


8389000
C
T


8389283
A
G


8393915
G
A


8396831
G
A


8397563
G
A


8402662
G
A


8411820
C
T


8413318
C
T


8414872
C
T


8431655
T
T/A


8441210
C
T


8449495
G
G/A


8453858
G
A


8463714
G
A


8466767
G
A


8491536
G
A


8497636
C
T


8506267
G
A


8509227
G
A


8515828
G
A


8529144
G
A


8544731
G
A


8546526
C
T


8556565
G
A


8559522
G
A


8560241
C
T


8567608
C
T


8572238
C
T


8576774
G
A


8582623
C
T


8589455
C
T


8589459
C
T


8595339
C
T


8610015
C
T


8622039
C
T


8631175
G
A


8640526
C
T


8646416
G
A


8647802
G
A


8648408
G
A


8655581
C
T


8658688
G
A


8668191
C
T


8668196
G
A


8669919
G
A


8674137
C
T


8678961
G
A


8682722
G
A


8690364
C
T


8691972
C
T


8699652
G
A


8699708
G
A


8709561
C
T


8720459
G
A


8731485
G
A


8737851
G
A


8737859
G
A


8742265
C
T


8743148
C
T


8754468
G
A


8754501
G
A


8759529
G
A


8761290
C
T


8769464
T
C


8792629
G
A


8793001
C
T


8809351
C
T


8813761
C
T


8813866
C
T


8816396
G
A


8821923
G
A


8825490
G
A


8828064
C
T


8841828
G
A


8842797
G
A


8843541
G
A


8845199
T
C


8848419
G
A


8849052
G
A


8854017
C
T


8854256
G
A


8858030
G
A


8859328
G
A


8860995
G
A


8861382
G
A


8871894
C
T


8872344
C
C/T


8875526
G
A


8881555
G
A


8889242
Ci
A


8893686
G
A


8894581
G
A


8896018
G
A


8901982
G
A


8902511
G
A


8902844
G
A


8902903
G
A


8907784
G
A


8927137
G
A


8929703
G
A


8931762
C
A


8934108
G
A


8937518
C
T


8943513
C
T


8943676
C
T


8944024
C
T


8945415
C
T


8946251
C
T


8948806
G
A


8950622
C
T


8951908
C
T


8953417
G
A


8955117
G
A


8956021
C
T


8956608
C
T


8958843
C
T


8959688
G
A


8974326
G
A


8979884
C
T


8982938
G
G/C


8982956
G
G/C


8982975
G
G/C


8983002
G
G/A


8983053
C
C/T


8983087
G
A/G


8983088
G
G/C


8983102
A
G/A


8983134
G
G/C


9008254
C
T


9028349
G
A


9029056
C
T


9045846
G
A


9049564
C
T


9052240
C
T


9052265
G
A


9053107
G
A


9058239
G
A


9060181
C
T


9064090
C
T


9072129
C
C/T.

2. A microorganism comprising the DNA of claim 1, wherein said microorganism is Actinoplanes utahensis.

3. A method for producing Acarbose comprising the steps:
a) cultivating the microorganism of claim 2 to produce Acarbose; and
b) harvesting said Acarbose produced by the microorganism.